LOCUS       DAWFBO010000001        56503 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFBO010000001 DAWFBO010000000
VERSION     DAWFBO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 56503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56503
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.001"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            52..1005
                     /locus_tag="WGO87_000001"
     CDS             52..1005
                     /locus_tag="WGO87_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0391440.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDEMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1122..1445)
                     /locus_tag="WGO87_000002"
     CDS             complement(1122..1445)
                     /locus_tag="WGO87_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391441.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1618..2979)
                     /locus_tag="WGO87_000003"
     CDS             complement(1618..2979)
                     /locus_tag="WGO87_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0391442.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAAHRLVMIGDAAERYQEDPVRILRAIRLSGKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAADRHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            complement(3334..5034)
                     /gene="recJ"
                     /locus_tag="WGO87_000004"
     CDS             complement(3334..5034)
                     /gene="recJ"
                     /locus_tag="WGO87_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0391443.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVSLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPARDITLEQAQNLAKHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5103..5819)
                     /locus_tag="WGO87_000005"
     CDS             complement(5103..5819)
                     /locus_tag="WGO87_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391444.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAV
                     GAVLALPVAALIAATNSSDK"
     gene            complement(5866..6378)
                     /locus_tag="WGO87_000006"
     CDS             complement(5866..6378)
                     /locus_tag="WGO87_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0391445.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MESQGGTGEA"
     gene            6532..7863
                     /gene="pmbA"
                     /locus_tag="WGO87_000007"
     CDS             6532..7863
                     /gene="pmbA"
                     /locus_tag="WGO87_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0391446.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8160..8363)
                     /locus_tag="WGO87_000008"
     CDS             complement(8160..8363)
                     /locus_tag="WGO87_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0391447.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8606..8908
                     /gene="clpS"
                     /locus_tag="WGO87_000009"
     CDS             8606..8908
                     /gene="clpS"
                     /locus_tag="WGO87_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0391448.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8910..11201
                     /gene="clpA"
                     /locus_tag="WGO87_000010"
     CDS             8910..11201
                     /gene="clpA"
                     /locus_tag="WGO87_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0391449.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLDRDLKNMVYGQENAIDALVTAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGF
                     TAKREHGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTPALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTALA"
     gene            complement(11241..14339)
                     /locus_tag="WGO87_000011"
     CDS             complement(11241..14339)
                     /locus_tag="WGO87_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0391450.1"
                     /translation="MSLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSKPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSTDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTG
                     EVRRGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEALNETA
                     AKRYLISSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(14336..15475)
                     /locus_tag="WGO87_000012"
     CDS             complement(14336..15475)
                     /locus_tag="WGO87_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0391451.1"
                     /translation="MGRSLTEIAEQLKGSNKKVQLIYAFNGTGKTRLSGEFKNLIAPT
                     SSEGADGEPTRRKFLYYNAFTEDLFFWDNDLLANEAPRLKIQKNSFTDWLLRDNGLDG
                     AVIKNFQYYTDDKLTPDFNDNFSEIVFYFARGNDEQIENIKISKGEESNFIWSIFYVL
                     IRQVIAELNIPEDSEEGRSTDQFDDLEYIFIDDPVSSLDENHLIQQAVDLADLIKSSE
                     SELKFIITTHNVLFYNVLYNELKLKNKNGSYLLLKNEDGSFDILEKRGDSNKSFSYHL
                     HLKGVIEKAIQNQQVERFHFILLRNLYEKTANFLGYEEWSNLLPEDGRQNYFQRIINF
                     TSHSTLSNEEFIEPTRQEQETIKLLLKHLLDNYNFFQSNEQRDNP"
     gene            complement(15518..16723)
                     /locus_tag="WGO87_000013"
     CDS             complement(15518..16723)
                     /locus_tag="WGO87_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0391452.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGEVAEIGTGSSNRQDEEHDG
                     EYPFYVRSKNILRSSTYQFNETAIIIPGEGGIGDIFHYAEGKYALHQRAYRVHVVIPD
                     LSPKFLYYFMNVDFKRYIQAKSVGATATSIRKPMLQRFPIPIPPLETQQKIVKILDKF
                     TELEATLEAELALRKRQYQYYRDFLLDFDNQIGGGADGYQCRLKDVVWKTLGEVTLPT
                     ENIKWKYTDKIYQYIDLTSVSREYKTITDTIAISANNAPSRAQKIVKEHDVIFATTRP
                     TLQRFTIISKKFSGEIASTGYCVLRANCKEVLPKWIYYQISTERFNQYVEVNQSGSAY
                     PAISDAKVKEFKIPIPSLPEQEKIVAILDKFDTLTHSISEGLPYEITLRRKQYEYYRE
                     QLLAFPKAA"
     gene            complement(16889..17899)
                     /locus_tag="WGO87_000014"
     CDS             complement(16889..17899)
                     /locus_tag="WGO87_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0391453.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(17896..19440)
                     /locus_tag="WGO87_000015"
     CDS             complement(17896..19440)
                     /locus_tag="WGO87_000015"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ0391454.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTKPKLKDSKPFDAVVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(19513..20517)
                     /gene="rfaD"
                     /locus_tag="WGO87_000016"
     CDS             complement(19513..20517)
                     /gene="rfaD"
                     /locus_tag="WGO87_000016"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0391455.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(20646..21677)
                     /locus_tag="WGO87_000017"
     CDS             complement(20646..21677)
                     /locus_tag="WGO87_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0391456.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(21679..22914)
                     /gene="dcm"
                     /locus_tag="WGO87_000018"
     CDS             complement(21679..22914)
                     /gene="dcm"
                     /locus_tag="WGO87_000018"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0391457.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(22951..23922)
                     /gene="hldA"
                     /locus_tag="WGO87_000019"
     CDS             complement(22951..23922)
                     /gene="hldA"
                     /locus_tag="WGO87_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0391458.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(23977..24717)
                     /gene="pyrF"
                     /locus_tag="WGO87_000020"
     CDS             complement(23977..24717)
                     /gene="pyrF"
                     /locus_tag="WGO87_000020"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0391459.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(25243..25890)
                     /gene="adk"
                     /locus_tag="WGO87_000021"
     CDS             complement(25243..25890)
                     /gene="adk"
                     /locus_tag="WGO87_000021"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0391460.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            26105..26944
                     /gene="htpX"
                     /locus_tag="WGO87_000022"
     CDS             26105..26944
                     /gene="htpX"
                     /locus_tag="WGO87_000022"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0391461.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(27247..27807)
                     /locus_tag="WGO87_000023"
     CDS             complement(27247..27807)
                     /locus_tag="WGO87_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391462.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(27921..28313)
                     /locus_tag="WGO87_000024"
     CDS             complement(27921..28313)
                     /locus_tag="WGO87_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0391463.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(28521..28913)
                     /locus_tag="WGO87_000025"
     CDS             complement(28521..28913)
                     /locus_tag="WGO87_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0391464.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(29030..29440)
                     /locus_tag="WGO87_000026"
     CDS             complement(29030..29440)
                     /locus_tag="WGO87_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0391465.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(29454..29891)
                     /locus_tag="WGO87_000027"
     CDS             complement(29454..29891)
                     /locus_tag="WGO87_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0391466.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(30303..31601)
                     /locus_tag="WGO87_000028"
     CDS             complement(30303..31601)
                     /locus_tag="WGO87_000028"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0391467.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(31705..32856)
                     /locus_tag="WGO87_000029"
     CDS             complement(31705..32856)
                     /locus_tag="WGO87_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0391468.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            33191..34570
                     /gene="norM"
                     /locus_tag="WGO87_000030"
     CDS             33191..34570
                     /gene="norM"
                     /locus_tag="WGO87_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0391469.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            34749..35789
                     /gene="murB"
                     /locus_tag="WGO87_000031"
     CDS             34749..35789
                     /gene="murB"
                     /locus_tag="WGO87_000031"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0391470.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            35801..36451
                     /locus_tag="WGO87_000032"
     CDS             35801..36451
                     /locus_tag="WGO87_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0391471.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            36506..37303
                     /locus_tag="WGO87_000033"
     CDS             36506..37303
                     /locus_tag="WGO87_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0391472.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPSGRRLKN
                     IIEPRHPTRREFFTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            37354..37920
                     /locus_tag="WGO87_000034"
     CDS             37354..37920
                     /locus_tag="WGO87_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0391473.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGRFSREPESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            37940..38830
                     /locus_tag="WGO87_000035"
     CDS             37940..38830
                     /locus_tag="WGO87_000035"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0391474.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            38855..39613
                     /locus_tag="WGO87_000036"
     CDS             38855..39613
                     /locus_tag="WGO87_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0391475.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(39668..40333)
                     /locus_tag="WGO87_000037"
     CDS             complement(39668..40333)
                     /locus_tag="WGO87_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0391476.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(40533..41429)
                     /locus_tag="WGO87_000038"
     CDS             complement(40533..41429)
                     /locus_tag="WGO87_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0391477.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            41498..42655
                     /locus_tag="WGO87_000039"
     CDS             41498..42655
                     /locus_tag="WGO87_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0391478.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFY
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(42718..43719)
                     /gene="hemB"
                     /locus_tag="WGO87_000040"
     CDS             complement(42718..43719)
                     /gene="hemB"
                     /locus_tag="WGO87_000040"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0391479.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEASNLGIPMLALFPVVTTNKTECAQEAYNPE
                     GLVPTAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(43902..44186)
                     /gene="yhbY"
                     /locus_tag="WGO87_000041"
     CDS             complement(43902..44186)
                     /gene="yhbY"
                     /locus_tag="WGO87_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0391480.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            44296..44916
                     /locus_tag="WGO87_000042"
     CDS             44296..44916
                     /locus_tag="WGO87_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0391481.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            44980..46947
                     /gene="ftsH"
                     /locus_tag="WGO87_000043"
     CDS             44980..46947
                     /gene="ftsH"
                     /locus_tag="WGO87_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0391482.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            47168..47599
                     /locus_tag="WGO87_000044"
     CDS             47168..47599
                     /locus_tag="WGO87_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0391483.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSVTLVEAFVKEADRRYA"
     gene            47592..47870
                     /locus_tag="WGO87_000045"
     CDS             47592..47870
                     /locus_tag="WGO87_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0391484.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            47923..48501
                     /gene="pth"
                     /locus_tag="WGO87_000046"
     CDS             47923..48501
                     /gene="pth"
                     /locus_tag="WGO87_000046"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0391485.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            48562..48768
                     /locus_tag="WGO87_000047"
     CDS             48562..48768
                     /locus_tag="WGO87_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0391486.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            48765..49127
                     /locus_tag="WGO87_000048"
     CDS             48765..49127
                     /locus_tag="WGO87_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0391487.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            49358..50773
                     /locus_tag="WGO87_000049"
     CDS             49358..50773
                     /locus_tag="WGO87_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0391488.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVALLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            50892..51401
                     /locus_tag="WGO87_000050"
     CDS             50892..51401
                     /locus_tag="WGO87_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0391489.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            51568..52950
                     /locus_tag="WGO87_000051"
     CDS             51568..52950
                     /locus_tag="WGO87_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0391490.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPK
                     NLQDLIVELKNGEAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(52970..53725)
                     /locus_tag="WGO87_000052"
     CDS             complement(52970..53725)
                     /locus_tag="WGO87_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0391491.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(53735..54451)
                     /locus_tag="WGO87_000053"
     CDS             complement(53735..54451)
                     /locus_tag="WGO87_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0391492.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(54441..55268)
                     /locus_tag="WGO87_000054"
     CDS             complement(54441..55268)
                     /locus_tag="WGO87_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0391493.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            55553..56461
                     /locus_tag="WGO87_000055"
     CDS             55553..56461
                     /locus_tag="WGO87_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0391494.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT        13556 a        15387 c        13757 g        13803 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatga gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt cgaccgtgtc
      661 accaagctga ttgaaaaagg tctgattgag attgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tatccgtttc tttatccgtc caaccgatac atcaatcgaa ttttctgcat
     1141 cggattgcct catcttcgca aaacattgcc gaagtttcgc ggttgcacgc ctgacgcact
     1201 ttctgccgcg ccacgccttc attccgatcg cttgcctcaa acgtcttctg aaaaggcgta
     1261 acgctgcata catacatggt ggaatttgaa ccgaaaaggt cgattttgac ggtattgctg
     1321 ccgctttcca atacggcgac acggtgttcc aactgcgcca gacgtgcttc caaatcgccg
     1381 attctgtcgg caaatgcagg catggcggca cacgcggtca aaatgcccaa aatacatttt
     1441 ttcatcaatt tttccttgtt tcttgaaccc tacccacccg gacatccttt ttccgagagg
     1501 cagggtcaga ctttatttac ggggatgaat ccccttacaa atcaggataa tacatagaac
     1561 ggcactttat gcttcatccc atgtgttgaa acttcattgc tccttgtctg ttgaccctta
     1621 ttcccaatcc gttccctcaa ccttcggctt gggtttgcgc ctgcgacggc ggcgtttttt
     1681 cgcctgtcct tcgttttttt cgtgtcggtc ggcggcttcg tttttggtca tctccgaccg
     1741 ctgttccgtc gatgccgtct gaaacgccgt ccaccactcg gcaagggcgc ggtccgcatt
     1801 gccggtttcg gcgcgcaaga gcaggaaatc ataggcggca cggaaacgcg cctgtgcaaa
     1861 cagtttgtgc ggccttgcgc ctttgcggtt ttcaaactgc ggctggaaca tccaaatttc
     1921 gcgcatcgtg gcggaaaagc gttgcggtac gccccaaccg cgttcgacgg tttcgcgcat
     1981 cgtattgatt gcatcggaca gggcgggcac gggtttcaaa ccctgttgca gattgctttt
     2041 ccaatggcgt tccaactccg gccacatcag ggccgccagt acgaaaccga ccgaaaccga
     2101 tttgtcggca cgcagccgct catcggtatt tttcagggca agcaccgtca tttttccggc
     2161 gatgccgtct gaaacgcgca aagcattgag cagcggatgg atgtcgtccg gtatgtcaaa
     2221 tccgttcaaa cgtttcagac actcgcgagc gtgccctgaa aacagcaatt tcataatttc
     2281 gtcgaacagc ctcgctaccg gttcgtgctt cagacggcat atcgattcgg caatcggtgc
     2341 ggcggtttct tccgacagat caaagcccaa tttgcccgac aggcggatgg cgcgcaaaat
     2401 cctgacaggg tcttcctgat agcgttcggc ggcatcgcca atcataacca gcctgtgggc
     2461 ggcaacatcg gcaatcccgt tgtggaaatc caaaatctct tctttttcag gatcgtaata
     2521 caaggcattg caggtaaaat cgcgccgcat cgcatcttct tcgatgctgc cgtaggtgtt
     2581 gtccttcatg attctgccgt gcgcgttctg atgaaccttt gcaccgccgc gaaacgtcgt
     2641 tacttcgata atctccgcac cgttcatcac atggacaatc tgaaaacgcc tgccgatgat
     2701 gcggctgcgg cggaagagtt tgtgcacctg ttcgggcgtg gcatcggttg ccacatcgaa
     2761 atccttgggt tcgatgccga gcagcaggtc cctgaccgcg ccgccgacca cataagcctg
     2821 aaaccccgcc cctttcaggc ggcgtatgac gttttcggcg gcaaagctca acatttcggc
     2881 acggatgttg tgtctttcgg caggaatgac cgttttactt tccgcttttt tactgctccg
     2941 accggaaggc agcatcttat tcaaccattt tttcagcata aatattctga cccgaagagc
     3001 ggcatctcca cgggcaaccg tgttcagacg gcatcgggcg caggtttgca agatgccgta
     3061 accataaaca aaaggcgtag aaactacgcc gtccgaatca cgccgccctc gcggcggcaa
     3121 cgcgtcatta taacagattg ccctccaaaa aaatcagacc ctgcttttgt ggcggagtct
     3181 gaaatttgat gcaccttata tattgcgtcc ctaagaaagg atgattaaca aaaattaacg
     3241 ccctttactt tcttttccga ttgggaacat tcccgtcgta caaacaggca aatgaaggaa
     3301 cagaaatcac attcaaacgg cgttccgccg cctctacgcc gcttcccagt agtcgatata
     3361 aagctgcaat tcgaggttgt tgcgccattc gttggcaacg gggcggtaca ccgtgcggat
     3421 gtattcgggg atgtcttcgc tgcaacgcca aaacatcgct tcaaattcgc agccgtcttt
     3481 ttgcagccag acttttttgt gtttgccctc cgcgcccaaa ggttgctggc ggacgacgtg
     3541 gaactcgtcg gtaaaactcg gcggcgcgaa gccctgcccc caaacgtgtt tagcaaggtt
     3601 ttgcgcctgt tccagcgtaa tgtcgcgtgc aggcaggctg ccgtcggtga tgaaggtttg
     3661 cgacaaatcg tcttcgcaca ccatttcgcg cacggcttct tcaaaggccg tctgaaacgc
     3721 gggaatgttg tgttcgagta tgctcaagcc cgccgccatc gcgtgtccgc cgaatttcaa
     3781 aatcaaatcg ggatggcgtt tggacaccaa gtccaaagca tcgcgcaagt gaagattggg
     3841 aatggaacgc cccgaaccgc gcacttcacc gttgtcggca ggcgcaaaca cgatggtcgg
     3901 acgataaaaa cggtctttga ggcggctggc aacgatgccg accacgcctt gatggaagtc
     3961 gtcgcgatac gccaccaaag tcatctgacc tgaaggcagg gtttcaggaa aagcattcag
     4021 cgcgtcttgc agcatagact gctcgatttc gcgacgttcg atattgaggt tgtttaactg
     4081 cgccgccagt tcctgcgcct cggtatcatc tcgcgccaac aggcaggcaa taccgaccga
     4141 catatcgtcc agccgtccgg cggcgttgat gcgcgggccc aacgcaaaac ccatatcaaa
     4201 cggctgcgcc ttgcgccaat cgcgccgcgc cacttcaaac aaggcgcgga taccggggcg
     4261 cattttgccg gagcgcatcc gtttcaaacc ttgcgacacg aggatgcggt tgttgtgatc
     4321 gagggagacg acgtcggcaa cggtaccgag tgcgaccaaa tccaaaaggt cgcccagatt
     4381 cggctctttt aggccgtctg aaaaataatt gcggcggcgc agttcggcac gcaaagccat
     4441 cagtacataa aaaatcacgc ccacgcccgc caagcttttg ctttggaaac cgcagccttt
     4501 ttggttcgga ttgacgatga tgcagtctgg cacggtctcg gcaggcagat ggtggtcggt
     4561 tacaatcaca tccaaaccca attcctgcgc acgcgccacg cctgcgatgc tggcaatgcc
     4621 gttatcaacc gtaatcagca aatccacgcc ttgcgcggcg gcgatttcgg ccagctcggg
     4681 cgttaaaccg tagccgtgtt caaagcggtt gggcacgagg aaatctactt ttgcccccat
     4741 cgccgccaaa ccgtccaaac cgacggcaca cgcggtcgcg ccgtcggcat cgtagtcggc
     4801 gacaatcaag attttttcct gacgctcaac cgcatccgcc aaacggcggg cggcggcttc
     4861 gcaattcgtc agcgtttgat aaggcaggag ggaagcgagt ttgtcgtcca attcggcagg
     4921 actttgcaca ccgcgcgagg cacaaagccg ggcgattaaa ggatcggcgc cggcggtgag
     4981 caaatgattg aaaacgtcgg tattgacgga tcgggtttgg attttgacgg acatagcgtg
     5041 gaactttaca gtgcggatgt gtttgaaatg ccgtctgaag gcttttcaga cggcattgcc
     5101 gttcatttgt ctgaggaatt cgtggctgca atcaacgccg cgacaggcaa ggccagcacc
     5161 gcgcccaccg catccagtat caacgtggtg ggcgtatatg caatattttc aaacaacttg
     5221 gatttgtcgg tatgtttttt cgtaaccgtg taataaatat cggcaggcac actttgctca
     5281 aaatgataat cggcgttcag tttttgcggt gtggcgtagt atttgccttt ggcggatacg
     5341 cagcgcgtgt aaatagtccg attgtcaagt tcgaccgctt caaacccgag ctgtttcagc
     5401 ttggcgatgt cggcaggctt gtcggtatcg tagcgcaggc aaaggccttc ggtactgaaa
     5461 ttctggctgc caggcgattc gagtttgacc ggcagggctt ggtggcgagc atagctcggg
     5521 gtatcctcaa ctatttggaa gggtttgtcc agccctgcct tcaaaatgcc cgtcagcttc
     5581 gccgaatctt cgggattgac gacgaaccag tatttcccgc ccatcatcac cagactgccc
     5641 ttttccaatt gggcattgtc tttggcaacc acaccgaagg cgcggatttg atccttgtca
     5701 acgtgtttat aggcggtcgt ttggctgaac gggtcgttca tgccccacat cattgccgta
     5761 cagccgttca acatcaaggc tgccgccaaa acggcggcgg tcgttttacg gaacaacatt
     5821 ttatcttcca atcagaatga cggcggatgc cgtctgaaaa tatgcctagg cttcccctgt
     5881 tccgccttgg ctttccataa cggttttcaa ctcttgaaac agggcgcgga aatttttcgg
     5941 cggtttgttc tgttcctgtt cttttttggt attgcggatg agcgtcctca gcttgcccgc
     6001 atcggcgttt ggaaaatcgg acataaactg cgtcagcgcg ccgtcgtccg ccagcagctc
     6061 catccgcgtc cgctccacac gttgcaaaaa ggcgttgtgc gccgcatcgt cgccgcgcag
     6121 cttggcaaga aatgcctcga tgggcgcggg atcggtatcg cgcatcagtc tgccgataaa
     6181 ttgcgcctgc cgcttgagcg cgccgttgga tgtgattttt ttataggtaa cgatggcttc
     6241 gtacaaatct tcgtccaagc cgattttttt caacgtgtcg cttgagagct tggtcagctc
     6301 catacccaaa tcctgcaaac cgttcatctg ctttttcatt tgggttttgc tgacccattc
     6361 gtcttcttgt tcaaacatct tcattctcaa attttatccg acgggcgcat tttaacagaa
     6421 tgattccgcg ttcagacggc attttgcacc gtccgccctg caaacggacg cttttgcccc
     6481 tgccgcccga aacggttaaa atgccgcaac gctccaccaa gaaagatttc tatgctgttc
     6541 aaccacaccg cttccgaact tctcgacctc tgccgccgca cgctcgactt ggcaaaagca
     6601 gcgggcgcga ccgccgccga agccgatttc agcgaatcca tcggacaaag cgtcagcgtg
     6661 cggctgggcg aaatcgaaca aatcgagttc cagcaggaca agtcgctgga cattaccgtt
     6721 tacgtcggca aacgcaaagg ccgcgctggc accgccgatt tctccgaaca agccctgcgc
     6781 gacaccgtcc gcgccgccgc cgacatcgcc cgccacaccg ccgaagacgg ttgcgccgga
     6841 cttgccgatg cctgtctgat ggcaaaacac atcggcgacc ccgaccttta ccacgaatgg
     6901 gatttggata cggaagccgc cgtcggcttg gcaaaacaat gcgaacaagc cgccctgaac
     6961 gaggatgagc gcatcgaaaa ctccgaaggc gcggcggtgc aaaccggcca ttaccaatac
     7021 gtttacggca acacccacgg ttttgccgca caccggcaag gcacgcacca cagcatttcc
     7081 tgcagcgtcg ttgccgccga cgaaaacggc atgcagcgcg actactggta cgattccgcc
     7141 tgccgccatc cggatatgga cagccccgaa accatcggtc aaaccgccgc ccgccgcact
     7201 ttgcgccgac tcggcagccg cagcatcccg acaggcagct accccgtcct cttcgatacc
     7261 accgtttcgg gcggtcttat cggacacctc gtcggcgcac tctccggcgg cgcgctctac
     7321 cgccaaagca gtttcctgat cgacagcatc ggcaaaaaag tcctgcccga tttcctcaac
     7381 ctgcgcgaag aaccgcacat cccccgctct ttccgcagca gctattttga tgcggaaggc
     7441 gttgccaccg cgccgcgctt cgtgattcaa aacggcattg tcgaaggcta tttcctcagc
     7501 agttacagcg cgcgcaaact cggtatgcag accacgggca acgccggcgg cgcgcacaac
     7561 ctgtatttga accatacgca cgaaacgcaa tccgacctgc tgaaagaaat ggggacggga
     7621 ttgttggtaa ccgaactgat gggacagggc gcgaacacca ttaccggtga ctactcgcgc
     7681 ggcgcggcgg gtttttgggt ggaaaacggc gtgattgcct accccgtcca cgagattacc
     7741 gtagccggac gcttgcagga tatgtaccgc gacattgtcg gcgtggcgga tgacgctttg
     7801 cggcgttcgt ccaacaaaat cggctcgatt ctgattgcgg ggatgacggt tgccggaagc
     7861 tgaacgccct gccgatgccg tctgaagacg aaggcagcac gttttgcccc gtgcctgccc
     7921 gtttgccgtt ttatgccgca ccgccttttg ccgacaacat tgcacatttt ttcatcagca
     7981 ctgattttcc cttatccgtc aaaatactaa gccacttgtc cacaacaggg tacacacaag
     8041 ttcaggataa ggcgtgcggg gattggggtt tttccgccgc cggcaaaacg gcagacaaaa
     8101 acggcgggac attgcctgtc ccgccgtttt caaatataca tttgaccgta catcaggaat
     8161 taagcagcct gaatgttggc ggcctgtttg cctttagggc cggtggttac gtcgaaagag
     8221 acacgttggc cttctttcag ggttttgaaa ccttccatat tgattgctga gaagtgagcg
     8281 aacaaatctt cgccgccttc atcaggcgtg atgaaaccaa aacctttagc gtcgttaaac
     8341 cattttacga taccggttgc cattagaaac ttcctatact caaaaattaa caaaatcagc
     8401 aaaacaaggc atacggcaaa ccgagccgtc tggcgttcct tccagcttga tatactcggc
     8461 ggatgcccaa tcctgcttaa ccgtcttttt acatttgtta agacaaaacg tcaagctatg
     8521 ttttcaaaaa agtgggaaaa taggcaaaag taccgaaaat acaacaaaaa tgccgtctga
     8581 acgcggcttt caaatgggac acactatgac cgcgcaacac caatccgaca cgctcttgca
     8641 ccgtctgaac accctgccgc cgaaacgtta cggcgttttc ctattgaacg acgattacac
     8701 cacgatggaa tttgtcgttg aaatcctgac cgaaatcttc atgctcggac aagaacaggc
     8761 ggtagcggta atgctcttgg ttcatcacga aggcaaaggc ctgtgcggca cttacacgcg
     8821 cgatattgcc caaaccaaac agcaacaagt catgcagcgg gcaaaagccg aagggcatcc
     8881 gctgcaatgt attgtcgagg agatttaata tgcttgcacc cgaattggaa cagattttgc
     8941 agcagcttta ccgcgaggcg cgtaaggctc attatgaatt tatcagcctc gagcatctgc
     9001 ttttggtact catcgaagaa gatgcctccg tgccgaacgt cttaaaactc tgcggcgcgg
     9061 atttgaaagt ggtgtccgaa cagctcgccg ccagcgttgc cgaaaacacc cccctgattc
     9121 ccgaacacct tttagacacg gtcgaaaccc ggcccacgct cggcttccaa cgcgtgatgc
     9181 aacgggcgat ggtgcatacc cagtctgccg gaaaagccgc agtcgaaccg ttggacgttt
     9241 tggtcgcgct gatgagcgaa accgacagcc acaccgtcta tttcctcaag ctgcaatcgg
     9301 ttacgcgttt tgaagttttg cgctgtattg cccacggctc tcccgatgaa gatgaagacg
     9361 atggcaacta ttcttcagac ggcatggacg acgataatga aaaccgcacc aaaccgggca
     9421 aaaacccttt atcggcgtac accgtcaacc tcaacgccga agtcaaagcc ggccgtatcg
     9481 accctttgat tggtcgcaaa cacgaaatgg aacggctggt gcaaatccta tgccgccgcc
     9541 gcaaaaacaa tccgcttttg gtcggcgaag cgggcgtggg caaaaccgcg ctggcggaag
     9601 ggctggcaca tcaaatcgtc aacggcggca ttccagacgc gctcaaagat gccgaagtgt
     9661 acgcgctgga tatgggctcg ctgttggcgg gcacgaaata ccgcggcgac tttgaagcgc
     9721 gggtcaaatc cgtcttgaaa cagctcgaaa aaatcccgca cgccattttg tttatcgacg
     9781 aaatccacac catcatcggc gcaggcagca ccagcggcgg caccatggac gcgtccaacc
     9841 tgctcaaacc cgcgctggca aaaggttcgc tgcgctgcat cggcgcgacc acttacgacg
     9901 aataccgcac cattttcgac aaagaccacg ccttaagccg ccgcttccaa aaaatcgacg
     9961 tggtcgaacc caccgtttcc gaaaccgtac aaatcctgcg cggcttgaag ccgatgtttg
    10021 aagccttcca ccaagtccgc tatacgcaag gcgcactcga agccgccgcc gaactctccg
    10081 cacgctacat caacgagcgt ttcctgcccg acaaagccat cgacgtgatg gacgaagcag
    10141 gcgcggcgca acggattctg cccaaatcca aacagaaaaa agtcatcggc aaagcacaaa
    10201 ttgaaaccgt catcgccaaa gtcgcgcgga ttcccgaaaa aaccgtgtcg cacgacgaca
    10261 aacaagtgtt gcaattcctc gaccgcgatt tgaaaaacat ggtttacggt caggaaaacg
    10321 ccatcgacgc gttggttacc gccgtcaaaa tgtcgcgctc cggccttgcc ctgcccgaca
    10381 aaccgatagg cagtttcctc ttctccggtc cgaccggcgt gggcaaaacc gaagccgcca
    10441 aacagcttgc ctactcgatg ggcgtaccgc tgcaacgctt tgatatgtcc gaatacatgg
    10501 aacgccatgc cgtatcacga ctcatcggcg cgcctccggg ctacgtcggc tttgaacaag
    10561 gcggcctttt gaccgaagcc gtcaacaaac agccgcattg cgtgttgctc ttggacgaaa
    10621 tcgaaaaagc ccaccccgac attttcaacg tcctcctgca agtcatggac gcaggcaaac
    10681 tgaccgacaa caacggcaag agtgccgatt tccgcaacgt catcctaatc atgaccacca
    10741 acgcgggcgc ggaaagcctc agccgccctg gcctcggctt taccgccaag cgcgaacacg
    10801 gcgacgaaat gcaggcaatc aacaagctct tcacgcccga gttccgcaac cgcttggacg
    10861 cgattatccc atttgcaccc ttgtccgaac ccatcatcgt caaagtcgtg gacaaattcc
    10921 tgctccagct cgaacaccag cttctcgaca aaaaagtcga agccgaattc acgcccgcat
    10981 tgcacaaata tctggcggaa aaaggtttcg acccgcaaat gggcgcgcgc ccgatgcacc
    11041 gtctgattca ggaaaaaatc cgcaaaccgc tcgccgacga actcctgttc ggcaaactat
    11101 ccgacggcgg cttcgtgcgg atagactggg atgcggcaaa agaagaagcc gtgttgaagt
    11161 ttaagaaaag caaggtcaaa ataaaaaccg ccctcgcatg aaatcaaaaa tgccgtctga
    11221 aagattcaga cggcattttt ttaaatatct gtccctattc cggcaaactt ctccacaaac
    11281 gccgcgattt tttggaagac actttgtttc ttcgtcagat attgcggatt gagcggactc
    11341 attttcggca gggtttcggt cagttccgtg ccgttttcgc tggcatagcc gcgtttgagc
    11401 gagctgatta aatagcgttt agcggcggtt tcgttcaggg cttcggcggc aatcaatcct
    11461 gccgcttcgt gccgcatcac ctcttgcgcg taggtgtaaa aggtttccag tatggcggga
    11521 acgtcgggca ctttgtccaa atccgtatcg ttgatgaaat ccacaatcag accctctttg
    11581 gcgcggtgtc cgatacttgc gcggatgatg cggcggattt cttccaccag ctccgctttg
    11641 cctttgattt ttttgtggtg ttcgaaaacc agttgcaaaa tgtaatccaa attgatttcc
    11701 tgcgatttga gcaaatccac ctcaaaaacc acatcgtccc aatcgatttt cgattgctct
    11761 ttctgttcgc ctgctttttg gcggcgcaac caatcgcgga tgtcgttgta ggcggaacgg
    11821 tagtcctgca ccgccctttc agacggcatc ggcacttgcc gcatttcctg cacgtcttca
    11881 tcgctcaagt agtatttttc ttgaaacgcc ttcatcgcat cttcgtccgc cgcgtccaca
    11941 ctctgcaact cgcgcagcgc ggcaaattca tcgtagttct gcaatacgtt ttccgcccgc
    12001 aggtattcgc cgaagagttt ggcaaaatct tttttgtctt tttccgtttc gattttgtcg
    12061 ggatcgggga aacgctcgcg caattctttt gccacatcca aataaccgcg ccgcacttcg
    12121 cccgtccgat catcgatata gccgttcatg tattcttcgt agcttttttc caacaccacg
    12181 tttttggtgt ttttatcgcc gaacagggta atcgcatcaa tggttgcctg ctccaaatcg
    12241 cggaagcaga caatattgcc gaaggttttg gtggcatcgt aaatgcggtt ggtgcgcgaa
    12301 aacgcctgca tcaggccgtg atagcgcagg tttttatcga caaacagcgt gttcagcgtc
    12361 ggcgcgtcaa aacccgtcaa aaacataccg accacaatca gcaaatccac ttcctggttt
    12421 ttcacccgtt ttgccaaatc gcggtagtag ttctgaaagg ctttgctgtc cgtgccgaaa
    12481 ttggttttga aacaggcgtt gtaatcgttg atggcagcct gcaaaaattc tttcgcgctg
    12541 ctgtccatcg cttccggttc aaaggtctca tcaacaattt caccgacggc gttctgctct
    12601 tcgttggccg caaaggaaaa aatggtggcc actttcagcg ggtgcaggct gcctgcctgt
    12661 tgtgttttga acgcttcgta atagcacttc gccgcatcca cgctgctgac ggcaaacatc
    12721 gcgttaaagc ctttgccacc cgcattcagc cggtgcgttt tctgcctgaa ctggttcagg
    12781 atatattgcg tgatttcgcg gatgcgttcg gggtgcagca gggcttggcg gttttcggca
    12841 gcactcagtt tcttctcatc ctgttccgct tccacggctt tgaactgcgg gcgcacatcg
    12901 ttgtaatcca ctttgaattt caatactttt tcatcgcgga tggcatcggt aatcacataa
    12961 gaatgcaact cacgcccgaa cacgcccgcc gtggtttccg cgcccaaagc gttttcggga
    13021 aaaatcggcg taccggtaaa gccgaactgg cagaattttt taaatttctt tttcagattt
    13081 ttttgcgctt cgccgaattg cgagcggtgg cattcgtcga aaataaagac aacctgctgg
    13141 tgataaaccg gcagattatc ttcactcttc atcaggttgt tcagcttttg gatggtggta
    13201 acgatgattt tgttgtcgtc tttttccaaa ttgcgtttca aacccgccgt gctttccgaa
    13261 ccgttcacgc tgtcggtcga aaaacgttgg tattccttca tcgtctgata gtccaaatcc
    13321 ttcctgtcca ccacgaagaa aaccttgtcg ataaatgccg attccgtcgc cagacgcgcc
    13381 gccttaaagc tggtcagcgt tttgccgctg cccgtggtgt gccaaacata gccgccgctt
    13441 tccggcttgc tccaattctt cgcctgcgcc gaactgttga ttttccacaa aatgcgttcg
    13501 gcggcggcaa tctggtacgg ccgcataatc agcagcgtat cattcgcatc gaacacgctg
    13561 taatgcagca aaacgctcag taatacgctt ttttgcagga acgtggcggt aaagtctttc
    13621 aaatccttaa tcggatgatt gtccgaccgc gcccaattca tcgtgaaatc gaagctgttt
    13681 ttgtcgcgct tggtggtgtt ggcgaaatag cgcgtgtccg tgccgttgga aatcacgaag
    13741 atttgcagga atttgaacag cgaattttcg ctgttgaagc tctctttgct gtaacggtgc
    13801 acctgattga atgcctcgcg caccgccaca ccgcgctttt tcaattcaat ctgcaccagc
    13861 ggcaggccgt ttaccaacac ggtaacgtca tagcggtttg catgcgtgcc cgtctgctca
    13921 aactggttga taagctgcac atggttgcgg gcaaggtttt tcttgtccag cagataaatg
    13981 tttttcagac ggccgtcatc aaaagcgaaa tcgtaaatat ggtcgtcgtg gattttgcgg
    14041 gttttatcgg taatattttc agacggtctg tccagatatt ccgtcaaaaa ccgcgcccat
    14101 tcgccgtctg aaaacgccac atcgttcagc cgctgcaact gcgcgcgcag gttttccagc
    14161 agcctgcttt ggctgttcaa atccttgcgg tattcgtaac cctgattctg caaatcggcg
    14221 attaactccg cttccaaccg gttttccgat tggtagctgc ccgactgttc gattttttca
    14281 tattggtcga gcacgatgaa attcggcgtt tcggcgatgg gtttggtttc gaggctcatg
    14341 gattatctct ttgttcatta ctttgaaaaa aattatagtt atccagcaag tgtttcagaa
    14401 gtaatttgat agtttcttgt tcttgccgtg ttggttcaat aaattcctca tttgataatg
    14461 tagaatgact tgtgaagtta ataatacgtt gaaaatagtt ttgtctacca tcttcaggca
    14521 gaagattaga ccattcttca taacctaaaa aattagctgt tttctcatac aggtttctta
    14581 gtaaaataaa atgaaatctc tctacttgtt gattttgtat agctttttca ataactcctt
    14641 ttaagtgaag gtgatatgaa aaacttttat tggaatcccc acgtttttca agaatatcaa
    14701 aactaccatc ttcatttttt aacaaaagat aacttccatt tttatttttc aactttagtt
    14761 cattgtatag aacgttgtaa aataaaacat tatgtgtggt aataataaac tttaactctg
    14821 attcgctact ttttatcaaa tcagccaaat caaccgcttg ctgaatcaga tgattttcat
    14881 ccaaagagct gacagggtca tcaataaaaa tatattccaa atcgtcaaac tgatctgtgg
    14941 aacgtccttc ttcgctatct tctggaatat tcaattcagc gatgacttgt ctgattaata
    15001 catagaaaat gctccaaata aaattacttt cttcaccttt ggaaattttg atattttcaa
    15061 tctgctcatc attaccacga gcaaaataaa atacaatttc tgaaaaatta tcattaaaat
    15121 caggagtcaa cttatcatct gtataatatt gaaagttttt aataacagct ccatccagtc
    15181 cattatccct aagcaaccag tcggtaaaac tattcttttg aatttttaat cttggagctt
    15241 cgttcgctaa caaatcattg tcccaaaaga ataaatcctc agtaaatgca ttatagtaga
    15301 gaaattttct tcttgttggc tctccgtctg ccccttctga actggttgga gcaattaaat
    15361 tcttaaactc tccggataaa cgcgttttcc ctgttccgtt aaaggcatag ataagctgaa
    15421 cctttttatt gcttcctttc aattgttcag caatctcagt taaacttctt cccattatat
    15481 ttccttatca atattttcag atgacattat cccaatttca ggctgccttg gggaaggcaa
    15541 gcagctgttc acggtaatat tcgtattgtt ttcggcgcag ggtgatttcg tacggcaggc
    15601 cttcgctgat ggagtgggtc agggtgtcga atttgtcgag gatggcgacg attttttcct
    15661 gttcggggag tgaggggatg gggattttaa attctttaac ctttgcatct gatattgctg
    15721 gataagctga gccactttga tttacttcaa catattgatt aaacctttct gttgagattt
    15781 gataataaat ccactttggc aatacctctt tgcaatttgc tcttaataca caatatcccg
    15841 tgcttgcaat ttcaccagaa aatttcttac taattattgt aaaacgttgt agcgttggcc
    15901 gagtagttgc aaaaatcaca tcatgttctt ttacaatttt ttgagctcta cttggcgcat
    15961 tatttgctga tattgcaata gtatcagtaa tggttttata ttcccgacta actgaagtca
    16021 aatcaatata ttggtaaatt ttatctgtat atttccattt aatattttca gtaggtaagg
    16081 ttacctcccc caacgtcttc caaaccacat ctttcagacg gcattgatag ccatcagctc
    16141 cccccccgat ttgattgtca aaatctaaaa gaaagtcacg gtaatactgg tattggcgtt
    16201 tgcgcagggc taattctgct tccagcgtag cttccagctc agtgaatttg tcaagtattt
    16261 ttacaatttt ttgttgggtt tctaggggtg ggatggggat tggaaatctt tgcaacattg
    16321 gttttcgaat agatgtagct gttgccccca cactctttgc ttgaatatac cttttaaaat
    16381 ctacattcat gaagtaataa agaaatttag gggataaatc aggtattaca acatgaactc
    16441 tatatgctct ttgatgtaag gcgtatttac cttccgcata gtggaatata tctccaattc
    16501 caccttctcc gggaataata atagcagtct cattgaattg atatgttgat gatctaagaa
    16561 tatttttaga tcgaacataa aatggatact ctccatcatg ctcttcatcc tgcctattgg
    16621 aacttcctgt accaatctca gccacctccc ccaacggctt ccactccact ggtgccgtct
    16681 gaatcatctc aatcaatttt ttcgctttgc tttgcatatc catggttgtt ttccgtttct
    16741 ttccctctgc ggcgggatgc ggggcggttg tggggtgctt cggctcactc tgtccgtttg
    16801 ccgttatttc tgcgggctga agtccgtcct gctttcaggc tgcctgcaat ggtgtgcagg
    16861 ctgctttttc aggtagcctg aatggtagtt attttgtgcg gtcttttttc tgtttgatct
    16921 gttgcacggc tgttttttcc aacgctttaa cgctttccag ataggcttgc tccaccggcg
    16981 acaggatgcg cgcttcatag gcgcggtatt cgcgttcggc tttctgctct gcctgtttgc
    17041 ggctgatgct gcctgcaccc tgtaatgtgc cttgtccgta cagcccggaa aatttgtcca
    17101 attcggctat ccagtcgcgc atatacatgg ggctttgctc ctgcgctttg atttccgcta
    17161 ggtcgaagaa ggcggaaacc agacggttca ggcggaacag ttcgtcttcg gtcagatagt
    17221 ttttggcgat tttggcttca ttcagcgtgg ggattgcgcc ttgaaaggtg gtcagcccca
    17281 taaagtcttt gctgctgtcg gcacggctgt atatcagctc agctgcggtt tgccggctgg
    17341 cggcatagtg cagtttgttt tgaacggcgg caaaaaaggt ttggctttcg ctgcttttgg
    17401 ggttgtagtc ttggctggtg gcatataaat caagcacttg ccggtatagg gctttttcgc
    17461 tgctgcggat gtcgcgaatg cggttgagca gttctttcca atagtcgccg ccgcctgtgc
    17521 ctttcaggcg ttcgtcgtct atggcaaagc ctttggtcag atattcgtcc agccgttcgg
    17581 ttgcccattg gcggaattgg atgccccgcg cggaacggac gcggtagccg acggcaataa
    17641 tcatgggcag ggaataatgg gcgattttgc ggtttacctt gcgcccgttt tcattttgaa
    17701 ctgtcaactg gaagttgaca gttgccttct cttccaattc ttgctctgca agaatggttt
    17761 taatgtgttt gctgatattt tgtttggtgg tttggtacag ttctgccatg tccgcctgcg
    17821 tcagccaaag ctgtccgcca aattcctgca aggcaaattg ggcagtgccg tcgttggcgg
    17881 tgtataggat gatgctcatg cttcaatctc tgcaatcact tcgtcaattt cacgccgcag
    17941 ccgttcgatt ttggcaacgg tttcgctgat ttcggcgtta agctgtttga tgtcgataat
    18001 ttcgcgggtg tcttcgggtt caacatagct gctgacggcg aggttgtagc cgttgtcttt
    18061 gacggtttgc tgggcggcgt tttgggcgat atgcggcaca tcggctttgt cggcgaagag
    18121 tttgacgatt tcggcaatgt gttcttcggt taagacgttg ttgttggttt cttttttaaa
    18181 gaagccgcct gcgtcgataa attggatgtc ggtattgtct ttgtgtttgg acaaaaccag
    18241 gatattgacg gcgatgccgg tgccgtaaaa gagattgggc gcaagggcaa tcacggtttc
    18301 cacgtagttg ccctccacca gatattggcg gattttctgc tctgcgccgc cgcgataaaa
    18361 aatgccgggg aaagagacga tggcggcgcg gcctctgccg gaaaggtagt tcagtgcgtg
    18421 caggatgaag gcaaaatcgg ctttggattt cggggcgagt acgcctgcgg gggcaaagcg
    18481 gtcgtcgttg attaaggtgg ggtcgtcgct gcctatccag ttaatggaat aaggcggatt
    18541 ggaaacgacg gcatcaaagg gtttgctgtc tttgagcttg ggcttggtca gcgtgtcgcc
    18601 caattcgatg tggaatttgt tgtaattgac gttgtgcaga aacatgttca ttcgggcgag
    18661 gttgtaggtg gtgtggttga tttcctgccc gaagaagcct tcttcgatga tgtgctcgtc
    18721 aaactgtttt ttcgcctgta agagcaggct gcccgagccg caggcgggat cgtaaatttt
    18781 atttaccttt tcctgcccgt gcaccgccag ccgcgcaatc agcttggata cgctttgcgg
    18841 ggtgaaaaat tcgccgccgg atttgcctgc gttggcagcg tagttggaaa tcaggtattc
    18901 gtaggcatcg ccgaaaaggt cgatgtggtg gtcttcaaaa ctgccgaaat cgagttccgc
    18961 cacgcctttt aggacggcgg caaggcgttt gttcttgtcg gcaacggtgc tgccgagccg
    19021 gctgctggtg gtgtcgaagt cgtcaaacag gcctttaatg tcttgttcgg acggatagcc
    19081 ggaggcggag ctttcaatcg cggtaaaaat ttctttcagc ttggtgttga gctcttcgtt
    19141 ttgatgggct tcggcggcaa tattgcaaaa aagctggccg gggtagatga aatagccttt
    19201 gactttgacg gcatcgtctt tgatttcggg cgtgatgatg ctgtccggca tagcggcgta
    19261 atcaatactg ctgtcgcctg cctgcatata gtcggtaaag ttttcgctga taaagcggta
    19321 gaaaagtgtg ccgagaacgt attgtttaaa gtcccagccg tccaccgcgc cgcgtacttc
    19381 gtcggcaatt ttccaaattt ggcggtgcag ttgggcgcgt tgttgcattt cggtcatcat
    19441 cgaaatccat ataaaagtta aacaaatcga aatcgcctga tattttcaga cgatttttta
    19501 cgggcattca aattaagcca aattttccag catccatttg acgtagcgct cgacgcctga
    19561 tttgacatcg aaaaattctt ccgtataacc tgcctcacgc aatttggtga tgtcggcttg
    19621 agtgaagctt tggtatttgc ctttgagcgc atcgggaaat gggatgtagc ggataagttc
    19681 ttcttctacc agctctttca agctcatttc gggtttgcct tcggcggcgc ggcaggcgtt
    19741 gacggtggcg gcggcgagtt cgttgaactg ttggctgcgg ccggtgccga ggttgtagat
    19801 gccggaaagt tcgggatggt cgaagaagta gaggttgact ttggcaacgt cttcgacgct
    19861 gacaaagtcg cgggtttgtt cgccgttgcc gtagccgtcg ttgctgccga acaggttgac
    19921 gtaaccgtgt tcgcggtatt ggtggaaatg gtggaaggcg acggatgcca tacggccttt
    19981 gtgttgttcg tgttgtccgt acacgttgaa gtagcggaag ccgacgactt gggcggtcaa
    20041 gccttctttc atgcggcggc gcaatacttg gtcaaacagg aatttggaat agccgtacac
    20101 attgagcggt ttttcgagtt cgcgctcttc acggaagatt tcgcctttac catacacggc
    20161 ggcactggag gcataaagga agggaatgcg ttcgtcctga caccagtcca acaaatccag
    20221 cgtgtactgg tagttgtttt ccatcatata caaaccgtcg tgattcatgg tgtcggaaca
    20281 cgcgccttga tggaaaacgg cttcgatgtt ttgataaggt aaaatgtgtt ccctcacttg
    20341 gcggatgaat tcatgtttgt cgagataatg ggcgatttcg cactcggcaa ggtttttgaa
    20401 tttttcgcct ttgctcaaat tatcgacggc aacaatgtca gtaataccgc gttgattaag
    20461 tgctttgacg atgttgctgc cgataaagcc ggccgcgcct gttacgatga tggtcatatt
    20521 cggtttcctt tgttttgagt aaaattaata aacttagtta caagaaatca tcgatattgt
    20581 cctgaaacac aaatttgcga tgatattcca aatatttttc tcttccttcg ggatatattc
    20641 tcatcttaat tttttgcatt tggattgata ttaagttttt cccatgtgta acggcttagt
    20701 cgagtaccgc aaatcaaacg accatcatcc aagaaagtaa tataaccttg atcgaacaac
    20761 caatcataag tcggcgttaa agctaaccca tttaaataat ctaatgcctc tttctcattt
    20821 ttctcagtaa tacaaaccat ataaggttta atatgactag cccttaacaa aatctcatct
    20881 gtaattaatg taaatggaca ttgcggcata tgatttataa catccttgcg atatttctgc
    20941 tgcccttttc tacttttctg tttaatgatt tcttgctcta cgaattgatt aaattctgaa
    21001 gccggtattt ctaatttttc tcttgataag agttgcgggt gcacgataga gcgaaattga
    21061 taatccaaaa atattcggaa ataaaataat ggttctgaat cttctatatc tttgacaggc
    21121 aatagtttaa gaattgataa ataactaatt ttgggcaata caatcttacg ccaaattgac
    21181 catatatcat cctccgaacg gatataaccc ctacggtaac ctttagaatt ttccaaatgg
    21241 tctgatacat catggattga aaagaaaata ttttctgtag gcagattatt aatacttaaa
    21301 acgtaatcat cataaaacct tgaaatatca tgatgatatt tctgttcttg tttgtgatat
    21361 tcaacttttg catcaaccaa ataacgctgt aaatttttct tattaaaaaa acaatttttc
    21421 tgaatgaaag gatattctac cctccttcca ttttttgtct cagtaatccc ccaattttca
    21481 tactcaaaaa atgtacttag ttttcgacca ctatcatcaa tataactacc tacatatttg
    21541 cgtgcttccc cattaccacc aaaaacagat aacttgttat tcctgtgaat aaaactatct
    21601 tccgcacgta aattttgaat actatcgaca ataaaatact ctatgttttc aatagtaatt
    21661 ctttgaaaat ccggcatttt actctccata aagcgtaata tccatttgtt ttaatatagc
    21721 gattaataca tcaacaacaa tactgtttcc cgcttggcga tacatttgag tatcactaac
    21781 taaaatttta aaatcatctc taaaacccat tagccgcaaa cattccctag gtgttaattt
    21841 acgaatacgt ccacgattat gagtaacata attatcaacc ccggcacgat gcattttatg
    21901 catagtttgc aataatgggc gagctacagg caaatcagtt tccgtactgg ttttaaaatt
    21961 ttttgttcct cctgccaaaa cataattttt gattttttca gaaagataat atttttcctc
    22021 aacgttattt acatcaaaaa taaagtcatc aaattcagat tcgcgggctg cctgaaaaat
    22081 aaaatcacca tgccaattaa attgttgatt tgctttttgg cataaggcaa tttctccatt
    22141 aatctgtgta taacgttttt gtctattttt agaactggta acaaatttca cacccttttc
    22201 acgtaaaaaa tatttgctat cagtatagtc ctccaaaaaa tcttgcatag tatgttctaa
    22261 ttcaatcttt tcaggaaact gaaaaccatt tataggaggg gtatgaaagc caacaacaaa
    22321 aatacgctca cgatgttgag gaatcccata atccttacta ttcattattt ggaaatataa
    22381 gtcataacca agtgaataaa aaacactttt tacaactttc caagtttttc cattatcatg
    22441 attaagcaag ccctttacat tttcataaat aaatatcttt ggttggactt catccacaac
    22501 gcgagcaaat tcatagaata atgttcctcg tgtatcttct aatcctgcac gtttgccaac
    22561 catggaaaat gcttggcaag gactgcctcc aactaaaata tcaacttgat ttctaaactt
    22621 tctcgcatca aattgagtaa tgtcgttatg ccaaaaatct tcatttaatt tatagtttcc
    22681 aagataactt tttttaacgt atggatcaat atctcctgaa aaaacaatgg tatggtttaa
    22741 atttaatcgg tggaatgcct gttcaaccgc accaatcccg ctgaatacgg ttgctaatct
    22801 aatatgtgaa tcaggtttaa gaaaagtttt agatttccaa ccttgttgac tgggaaagag
    22861 caaagttttt tgtaatcgag tatcgtgtgt ctgtgccatt gtcgaaatag tcatacttat
    22921 atcgttctgt ttatcttatc aatatgaaaa ctacattgtt gattgccctg acaatgcctt
    22981 gatcaattcg gcaaacgagc aaaccgccgt accgagtttc gccacgacaa ccccggccgc
    23041 agtattggca aggtacatgg cttcgggcat ggtgcagcct gccgccaaac ccaagcccat
    23101 tccggcaatg acggtgtcgc ccgcaccgga tacgtcgtaa acttcttggg cgcgggtggg
    23161 ctggtaaatc ggttcgcctt cgctgaacaa ggtcatgcct tcttcgcttc gggtcagtaa
    23221 aacggcggtc aggtcgaggt ggcggcgcag gttttgcgct ttttcggtca gctcgctttc
    23281 gtttttccaa ctgccgacca cttctttcaa ttcggcgcgg ttaggcgtaa tcagagttgc
    23341 accgacatat ttttcgtaat cgtcgccttt ggggtcgatt aagacggttt tgccggcgtg
    23401 tttcgcccaa tcgatcatat cggagatatg cgacaggccg cctttgccgt agtctgaaaa
    23461 aatgattgcg tcgtattcgg gcaagatttc gcggtatttc tgcttgattt gttccaacac
    23521 ttcgcagttg ggatgttctt caaaatcaag acggataagc tgctggttgc gggcgacgac
    23581 gcgcagtttg acggtggtgg cgatttgttt gtcgcgcatc agataggagg cgacgccgtc
    23641 ctgcaccatc agcgcatcga gcgcgtcggc ggcttcgtcg ttgccggtta cggacaacag
    23701 ccctgccctg ccgcccaacg aagcgatgtt gcgcgcgaca tttgccgctc cgcccgcgcg
    23761 ttggtcgatt cgtccgattt tcgccaccgg cacgggggct tcgggcgaaa tacgggacac
    23821 atcgccgaac caatagcggt cgagcatcac gtcgccgaca accaggactt tggcttgcgc
    23881 gaaacgggat ttgagggttt cttgttggaa cttggcggac atattgcctc cgttgtattt
    23941 tttcagacgg cctcagcctg taaggccgtc tgaaaatcag tttgcttcaa ggtttgccac
    24001 gcggttcact tcgcgcaata cggctaccgg atcggcagct tgggttacag gacgacccat
    24061 taccaaataa gtcgaaccgg cagccaaggc ttcggccggt gtcatgatgc ggcgctggtc
    24121 atcattattg ccggcaacgt ccaagcggat gccgggcgtg accaagacaa aatcctgtcc
    24181 caattcgcgg cgcagcggcg cggcttcttg ggcggaacag accacgccgt ccaagcccga
    24241 actttgcgcc agttttgcca agcggatgac ttgttcttca ggggcggtgt tcaaaccgat
    24301 ttccgccaaa tcgctttgtt ccatgctggt caacacggtt acgccgatta agagcggctt
    24361 cgtgccgtat ccggcaacgg cttctgctgc ggcttccatc atacggcggc cgcccgatgc
    24421 gtgcatatcg accatccaaa cgcccatatc ggcagcgact ttgcaggctt gcgcgacggt
    24481 gtggggaata tcgtggtatt tcaaatcgag gaaaagtttg aaaccttgat tgattaagct
    24541 ttctgccaaa ttgcgtcccg tcgcggtaaa cagctctttg ccgattttga tttgacacaa
    24601 tgtcgggtca aggttgcgga cgaatccgag cgtgtctttt tcgttggaaa aatcaagggc
    24661 gacgatgacg ggggtgcgtt gttgcggagt ttggaagtcg gagattaaag gattcatggt
    24721 gttttcgttt cgggttttca gacgaccttt atcgtctttt tcgcagtata gtggattaac
    24781 tttaaaccgg tacggcgttg tctcgcctta gttcaaagag aacgcttctc taaggtgctg
    24841 aagcacaaag tgaatcggtt ccgtactatc tgtactgcct gcggcttcgt cgccttgtcc
    24901 tgatttttgt taatccacta taacaacgta aattcaaacc gtaggtcggg cattgatgtc
    24961 cgacctacgc ggactcaagc ccccgacaaa ctgtttcgga taaatttgaa ccggtacatc
    25021 agccgtcatt cccgcgcagg cgggaatcca ttgataaaac tcagttcatc agatttaaac
    25081 agcgattgcc caaattcaga aatggattcc cgcctgcgcg ggaatgacgc caaatgatta
    25141 tttttcggtt gcaacacgct gtttatcaaa aacaggtcgt ctgaaaacct gattttctgt
    25201 tttcagagtg cgaagcctgc ccgtgggtac gaccttttcg atttatttgc ccaatgcgcc
    25261 caatacttcg gctttcacgg cttctaccgg ctgagtgccg tcaactttga tgtatttagg
    25321 cgcgtgttcg ccttccagtt tgctgtaaaa atcaaccaaa acttcggttt gctcgtggta
    25381 aacggcaagg cgttttttca cggtttcttc tttgtcgtcg tcgcgctgaa tcaaatcttc
    25441 tccggttacg tcgtctttgc cttcaacttt gggcgggttg taggtaacgt ggtaagtacg
    25501 gccggaagcc aaatgcacgc ggcggccgct catgcggtcg acaatcacgc tgtcaggcac
    25561 gtcgatttca acgactgcat ccaaatccac gcctgcttca accatcgctt cggcctgtgc
    25621 cagcgtgcgc gggaaaccgt cgaacaggaa accgtttttg cagtcgtctt gcgcgatgcg
    25681 ttctttgacc atgccgataa tgatgtcgtc gcgcaccaag ccgccttcgt caatgatttt
    25741 tttcgcttcc aaacccaagg gcgtgccagc cttaatcgcg gcgcggagca tgtcgccggt
    25801 agagatttgc ggaatgccga acgcggcggt gatgaattgc gcctgagtgc ctttgcccgc
    25861 gcccggcgcg cctaaaagta atgctttcat gaagtgatcc tttgaaatgt gtttattgga
    25921 atcaaacaga tggtttacga ttctacacgg cttggcacaa tagttcaaaa accaactgca
    25981 caaactgcca tttgcaagca aatgttttgc atacctttgc cgagtctgat attcacaagc
    26041 cggcaacttt ttgggacaat ccccaaaatt tcaaaacatc acaccctacc aaaaaggaac
    26101 atccgtgaaa cgaatttttc tgtttttggc taccaatatc gctgttttgg tcgtaatcaa
    26161 cattgttttg gcggttttgg gcatcaacag ccggggcggc acgggcagcc tgttggcgta
    26221 ttccgccgtc gtcggcttca ctggttcgat tatttcgctg ctgatgtcca aattcatcgc
    26281 caaacaatcg gtcggcgcgg aagttatcga cacgccgcgc accgaagaag aagcctggct
    26341 tttgaacact gtcgaagccc aagcgcggca atggaacctg aaaacgcccg aagtcgccat
    26401 ctaccactcc cccgaaccca atgcctttgc cacgggcgca tcgagaaaca gctccctgat
    26461 cgccgtcagc accggtttgc tcgaccatat gacgcgtgac gaagtggaag ccgtattggc
    26521 gcacgaaatg gcacacgtcg gcaacggcga tatggttacg ctgacgctga ttcaaggcgt
    26581 ggtcaatacc tttgtcgtgt tcctgtcgcg cattattgcc aacctgattg cccgaaacaa
    26641 cgacggcagc cagtcccagg gaacttattt cctggtcagc atggtattcc aaatcctgtt
    26701 cggcttcctt gccagcttaa ttgtcatgtg gttcagccga caacgcgaat accgcgccga
    26761 cgcgggcgcg gcaaaactgg tcggcgcgcc gaaaatgatt tccgccctgc aaaggcttaa
    26821 aggcaacccg gtcgatttgc ccgaagaaat gaacgcaatg ggcatcgccg gagatacgcg
    26881 cgactccctg ctcagcaccc acccttcgct ggacaaccga atcgcccgcc tcaaatcgct
    26941 ctgagccgat ttgaaacaac aaaaaaaccg tacgcgcaag cagtacggtt ttttgtatat
    27001 gctgtctgaa aacatcaggt ttccataccg acaccaaccg tatccatttc cgcaccggat
    27061 acgccgcatt gatataccct gataaaccgt atccggacag aaaatatagt ggattaacaa
    27121 aaaccagtac ggcgttgcct cgccttagct caaagaaaac gattctctaa ggtgctgaag
    27181 caccaagtga atcggttccg tactatttat actgtctgcg gcttcgtcgc cttgtcctga
    27241 tttttgttaa tcccctatat cagataaaac agcggtaaaa ttgccgtctg aaaccaactc
    27301 tgcgccatac gggacacgct ctgcacgtct atttgcctcc gatatatcct ccctactgta
    27361 ggaacgtctg ccgatttgca cgaaatcggc acctctagca cccttcagga tggcggacgt
    27421 tatccgttct tgggattctg caagacggtc gacggcatta tacagacgct cggtctgata
    27481 cccttccgaa gcaacctgct tcaggtattc caaataaccg taacccaacg cccctccgac
    27541 aagcagggtc gtcaacgcta ccagcgttgc cgaacggatt ttagggggca tatcttgaag
    27601 catatctcta ccgaaccgaa ccaaagtctc tttaaagtcg gactctacac gcgtagaacc
    27661 ctcggaaatc ttgaagttca actcatactg cttccgtttg tccgctgtca accgcttgag
    27721 gttgtcatcc ccgtattgga cggttgacac aaccctgtac agactgtcct gtatttcgac
    27781 cagcgtctgt gcaaagttgg cggtcataac tggattccca acccttgccg ccgattctga
    27841 tactcaaaac ggtatccagg cgttccagca gggcttcggg agacagcagt gcgtattctt
    27901 caatattttc cataggttga ttatagcaga accttatacc tgttacagac aaggttgtcg
    27961 ggatttgaac gtccgtcgag cttggttcgt atagagccgt agggggtgtt tagggctttg
    28021 gcggcagctt gtacgctggg gaacgtgagt gtttcgcctg tggcggtgtc gaggatggag
    28081 acaggttttt ggttttgggg aggcgtattt tccttcattt taagtttccc gcactccgga
    28141 cacccatact ttgactttat agctgtgtcg taatacaaaa gttggacaac cccgtgcgta
    28201 ggacatctaa cactggaagg tttataaatg ccactgaact ccaacaattc taaatgtgga
    28261 aatttctctt gaaagcgtga tttggcggta tcgaaagtca ttcgcttacc cataatttgt
    28321 ctccataggt tatgtgcaaa tggattacac aaccaaaata tagaaatttt caaatttttt
    28381 gagacctacc tgaaatcctg cgcaagcaca atgtaaagaa aaatggtata cgtctttact
    28441 aaatctttac ataaattaac tctaaccggg atttgaacgt ccgtcgagct tggttcgtag
    28501 gatttagggt gttgtgaacc ttacctatcg tacccaagca ctttgtacct gtttgaaata
    28561 aggttgtcgg gcgacgttcg acccttgagc ctgtgattga tttgttggaa atgtacacct
    28621 aaagcagcac ctgcggcagt aacgcttggg aacgtgagtg tttcgttggt cgttgtgtcg
    28681 aggatgagga ggggcttccc tacatttgga ggtgtttggg tttttgacat cttagcacac
    28741 tcaggacacc cgtactttga ctttataagg gcattaaatg tcgaataacg aacgttcccg
    28801 tgcataggac aattaacggt tactgggtaa ttcacaccgt caaagtcggt tagagagata
    28861 ttagggaata tatcagtaag ttcagaagct gcttggttga aagatttggc catttgagac
    28921 tccaaaattg attgtcaaat ggatttaata gcaaatttat aaaaatgtca aatttttcga
    28981 gaagcacctg atagtggcga ggggtataca ctgttaaaaa gtgtatacgt catttagtgc
    29041 agatgtgaac cttatacctc cctgcaagta aagtctctgt gtgaacccga ccttttatac
    29101 ggtcagtgat agctgctggg tttataccca atgaccttgc agcagatgct cttgagggga
    29161 atgtaagtgt ttcgccagtt acagtatcct ccaatactgt atttttagat aatttatcta
    29221 ttgtatcttc tggaattttg tgggaattga caccataaga tgcacatttt ggacacccac
    29281 ttttagagtc caacatactt ttgaatgtag agcaagtaac cctcccgtgt ataggacaga
    29341 cgattaccgt cgggtaacga acgccattga actccacaag attcaaatgt ggaaatttag
    29401 aggttagctt agaaactgct tgagtaaatg taagtgccat agtggttgtg aacttacttt
    29461 accaacctat aacgatacct aatcaatcgg tcaaacggag tgcgtccgct taaacgtaca
    29521 gctaaagtgc tgttggagca accgataaat ttactggcag ccagtgtact tgcaaactcg
    29581 tagatttccc ctgtctctgt gtcctccaac ctaatggcaa cggcgtttgg agagggcttt
    29641 tcgggatttc gagtacattc aggacatcct gttgaggatt tcatggcagc ttgtagattc
    29701 ccgtaaacaa ccgacccatg caaagggcat tggattgtta ccggttcgta gtttttagtg
    29761 aacttcaaaa ccgtcttatc aggaaattta ctggaaaacc ttaactgaac atcttgtagc
    29821 gagagtgatt ttggcattat agacctttct aaaatgaaag cattatacaa ccaaaatata
    29881 gaaattttca aattttctct gaacgcactg gtatccgatg gagctaaatg taaagaaaaa
    29941 tggtatacgt ctttactaaa tctttacaca ttttaactgt atagtgtttg acaaacaaca
    30001 ggcataggcg cataatgcac ccgtagttag atagatagta aggagataac caatgactta
    30061 gcacctgccc ccctctctct cgcgcacccc gcccctcgct tgcggctaat gggctttgcg
    30121 cgacatatac actttcatct cctgcatggc ttaactgcct atgcaatagt acacacatca
    30181 acaaatcagg ctggcacgtc ttggcattgg ctgacgtacc attgtagccc gaaatgttga
    30241 taccctacta tttatgacca acggcggatt agggcttcag acggcaattt tactgcaaaa
    30301 ccttatgcga acggatgatg cagcacaatc gtttcttcgc ggtcggggcc ggtggagacg
    30361 atggcgaccg gcgcaccgca gacttcttca atccgtttca aatatgcttt ggcattggca
    30421 ggtaatgcgt cgtagctttt cacgccgaaa gtggattcac gccagccggg catggtttcg
    30481 taaatcggct tgcaggtttc caccgcatcg gaaccgcaag gcaggatgtc ggttttgccg
    30541 ccgtcgggca attcatagcc gacgcagata ttgatggttt caacgccgtc cattacatcg
    30601 agtttagtaa tacacatacc ggaaatgccg ttgatttgga tggagcgttt caatgcggcg
    30661 gcatcaaacc agccgcagcg gcgtgcgcgt ccggttaccg aaccgaattc gtgtccgcgt
    30721 tctgccaaac ctgcgccgac ttcgtcgaac aattcggtcg ggaacgggcc cgaaccgacg
    30781 cgcgtggtat aggctttgac gatgcccaaa acataatcca gcatttgagg acctacgccc
    30841 gcgcctgcgg aagcggctcc cgccagacag ttggacgagg taacgaaggg ataagtgccg
    30901 tagtcgatgt ccaacaacgc accttgcgcg ccttcaaaca gcagtttttc gccgtttttg
    30961 tttttctcgt tcaacacgcg ggacacgtcg gtaatcatcg gcgcaatgcg cggcgcgact
    31021 ttttcgataa ccgccatcac gtcttccgct ttaaccggct cggcattgtg cagatgttgc
    31081 agttggacat tgtaataggc aaggacggca tccagttttt cacgcagttt ttcaggatgc
    31141 agcaaatcgg cggcgcgaat ggcgcggcgt gccactttgt cttcgtaggc agggccgatg
    31201 ccgcggccgg tcgtgccgat tttgcctttg ccgcgcgatg cttcgcgggc ttggtcgagc
    31261 gcgatgtggt aaggcaggat cagcgggcag gtcggcgcga ttttcagacg gccttcgacg
    31321 tttttcacgc ctgccgcgtt caactcgtcg atttcgccca acagggcttc gggggagacg
    31381 acaacgcccg aaccgatgaa gcagtccaaa ctttcatgca ggatgccgct cggaatcagg
    31441 cgcaaaatgg tttttttgcc gccgacaacc aaggtatggc ccgcattgtg gccgccttgg
    31501 aagcgcacca cgccgccggc ttcttccgcc agccagtcaa cgattttacc tttaccctcg
    31561 tcgccccact gtgcgccgat tactacaaca tttttagcca tagccatata acctatcgat
    31621 attaaaaatt attgcggaaa tccccaaacc ggtgcggctt tgcgccccgc cacgatttgc
    31681 ctttcatccg ccatgaacgg gtatttagcg tttcacgacc tgccaaacgc cgtccgtctt
    31741 tttcagacgg cctgcaagct cttccgaaac attgtgtccg ataccgtagt caatcacgac
    31801 acactgccct tgttcacgca aggcttcgac tgcttcgcgc gccgcttcgg catcttccgc
    31861 atcgaccaac acggcgggct gccgttcgat ggtgggcaaa cgcccgataa agctgcgcaa
    31921 gtcgaaactg aatcccgttg ccgggcgcgc cctgccgaaa tatccgccca atccgtcata
    31981 acgcccgccg cgcgcgaccg cgtcgtggaa atcggcggca taggcggcat acagcaagcc
    32041 cgtgtggtaa ttgtcgacac gcagctcgga caagtcgata tggatttcac aatcggggaa
    32101 tgcgtcgcac accgcctgca attcgcccaa cgcgccgccg accgccgaca aatccggcaa
    32161 ccgtccgcgc gcgtcggaca acacttcacg cccgccgtac aggcgcggca acagcgagaa
    32221 tgcttttgcc cacatgccgt ccagcttcca agccttgacc tgcgcttcga ccgccccggt
    32281 atctttatcc tgcatcaagg caagcagcgt tgcggactgc cccgcatcca aatgtgccgc
    32341 atcggacaag gcgcgaaata tgccgatatg ccccagcgaa agcagcactt tgcccatatc
    32401 ggcaattttc atgcttttca gcataaggtc tatcagctcg atgtcgccac ggatgtcggc
    32461 aaaaccgtac atttccgccc ctgcctgcaa gggttcgcgc atattcagca gaccgtcggg
    32521 ctgcgcgtgc aacaccggac cggcataaca caaccgatta atcccttggt tggcggataa
    32581 aagatgggca tcgatacgcg ccacctgcgg cgtgatgtcg gcgcgtatgc ccaactgcct
    32641 gccgctgagc ctgtccgtta ccaaaatggt tttcagggaa agccccgcat cgatatgcgt
    32701 cagcagggaa tgtgcgtact ccatcagcgg aggctgtacc agttcataac cgtgtacgcg
    32761 gaacagtgcc aacaactgct ccctcgcgct ttcaagctgc cgcgcgttcg tgggcagtac
    32821 gtcggcgata tgttccggaa gctgccatgt ctgcatgaga atctgccttc tattttatca
    32881 tgtaaataca aaaggctgcc cgatccgcaa tcagacggca tctttgccgc aacgcatcac
    32941 gccgctgtca aaacggcgat gcttccttcc gattcagaat ccgaacagcc cctgtccgac
    33001 ggctcaatgc cgtcaagtcg ttaaaaccaa actttaccat aaaatacaca caatctgaat
    33061 atacttttat accctgtatc cgccgtctga cggcacggca tttttttact atataatgtg
    33121 caccattatt tctcatcact tcctgctgcc gttttctgct tcagacggca tttttgttga
    33181 ttgaacactt atgctgctcg acctcaaccg cttttccttt tccgtcttcc tgaaagaagt
    33241 ccgcctgctg accgctcttg ccctgcccat gctgttggcg caggtcgcgc aggtgggcat
    33301 cggttttatc gatactgtga tggcgggtgg tgcaggcaag gaagacttgg cggcggtggc
    33361 tttgggcagc agcgcgtttg ccacggttta tattaccttt atgggcatta tggcggcgct
    33421 gaacccgatg attgcccagc tttacggcgc gggtaaaacc gacgaagtgg gcgaaacggg
    33481 gcggcagggg atttggttcg ggctgttttt gggcgtgttc ggcatggtct tgatgtgggc
    33541 ggcgattacg ccgttccgca actggctgac cttgagcgat tatgtggaag gcacaatggc
    33601 gcagtatatg ctgttcacca gcttggcgat gccggcggca atggtacacc gcgcactgca
    33661 cgcctacgcc tccagcctga accgcccgcg cctgattatg ttggtcagct ttgcggcgtt
    33721 tgtgttgaac gtgccgctga actatatttt cgtttacggc aaattcggta tgcccgcttt
    33781 gggcggcgca ggctgcggac tggcgacgat ggcggtgttt tggttcagcg cgctggcatt
    33841 gtggatttat atcgccaagg aaaatttctt ccgcccattc ggactgacgg cgaaattcgg
    33901 caaaccggat tgggcggtgt tcaaacagat ttggaaaatc ggcgcaccca tcgggctgtc
    33961 ttattttttg gaagccagcg cgttttcgtt tatcgtgttt ttgattgcgc ctttcggcga
    34021 ggattatgtg gcggcgcagc aggtcggcat cagtttgtcg gggattctct atatgattcc
    34081 gcaaagcgtc ggctcggcgg ggacggtgcg catcggcttt tcgcttgggc ggcgcgaatt
    34141 ttcgcgggcg cgttatattt cgggcgtgtc actggtgtta ggatggatgc tcgccgtgat
    34201 taccgtgctt tccttggtat tattccgttc gccgctggta agtatgtaca acaatgatcc
    34261 ggcggtttta agcatcgccg ccaccgtctt actgttcgcc ggcttgttcc aaccggcaga
    34321 cttcacccaa tgtatcgcct cctacgcctt gcgcggctac aaagttacaa aggtgccgat
    34381 gttcatccac gccgccgcct tttggggctg cggtctgctg ccgggctacc tgctcgccta
    34441 ccgtttcgat atgggcattt acggcttctg gacggcattg attgcctcgc tcaccatcgc
    34501 cgccatcgcc ttggtgtggt gcttggaatt gtgcagtagg gagatggtca gatcgcataa
    34561 ggccgtctga aaactccgcc gattaaaaat atagtggatt aacaaaaacc agtacggcgt
    34621 tacctcgcct tgccgtacta tttgtactgt ctgcggcttc gttgccttgt cctgattttt
    34681 gttaatccac tatagaaacg atgggctgaa gcccgccact ggcaatactc attttaaagt
    34741 tcaaacccat gcaacccatc cgataccgaa ccgaccttac cccctacaac actttcggcc
    34801 tgcgcgccca agcccgagcc tttattgcgc ttgaacacgc cgacgggttg cgcgacatcg
    34861 tccggctacc cgagttcaac cgagatactg ttttatggct gggcggcggc agcaatatcc
    34921 ttttgatgga ggactacgcc ggtctggtcg tacatatgga aaacaaaggc atacgcgaaa
    34981 ttgctcgttc agacggcatg gttctgattg aagcgcaggc gggcgaaatc tggcacgatt
    35041 ttgtcctgca cgccgtcgcg ctgggtttga acggtttgga aaacctgagc ctgattccgg
    35101 gtacggtcgg cgcgtcgccc gtgcagaaca tcggcgcata cggcgtagag gcgaaagatg
    35161 tgattcacag cgtgcgctgc ttcgatttgg atacggaaac ctttgtaacc ctttccaatg
    35221 ccgactgccg cttcgcctac cgcgaaagcc tgttcaagca ggaaggtaaa gggcgttatg
    35281 tgattgtttc ggtcgtattt gcattaaaaa cgcattttgt gccgaacttg ggttacggcg
    35341 atttggcggc cgccgttgcc gaactgagcg cgggcagggt gccgacggcg aaagatgttt
    35401 ccgatgcagt gtgtgcaatc cgcaacagta aacttcctaa tcctaacgtg cttggcaatg
    35461 tcggcagttt ctttaaaaac cccgtcgtca gcgcagaaaa agccgccacc ttgttgcagc
    35521 ggcatcctga tatgccgcgc tatccgcagc ccgacggttc ggtcaaactc gccgccggct
    35581 ggctgatcga ccaatgtcgt ctgaaaggct ttcaaatcgg cggcgcggcg gtacatgaca
    35641 ggcaggcttt ggtcttagtg aacaaaaaca atgcctcggc aaacgatgtc cggcaattgg
    35701 cgcaacacat caaatttaca gtatttgctc ggtttcaggt agaattgcac gccgaaccca
    35761 attggctgcc tgcttcgttc agcctgtaaa tccaaggagt atgcccgtga cccgccccgc
    35821 caaaatcaat acttacacac gcatcatcga cgccagcctt gcgcttttca acgaggaagg
    35881 cgagcgcaac atcagcacca accatattgc cgcccacttg ggcatcagtc cgggcaacct
    35941 ctattaccac ttccgcaaca aagacgaaat catcgtccaa ctgttcaaac gttacagcga
    36001 agccctgctg gaatacctca acgaagccgt gttgccgtct gatgtggaag actccatcaa
    36061 ttatatggcg ggcatttacg acgtgatgtg ggaataccgc ttcctattca gcgacgtgaa
    36121 caccctgctt gcacgcagtg ccgaactatt gggcgaacac aataccttca cccaagccaa
    36181 agtctccccg ctcttggtca acctactgac ccagctcaac ggtctgaaca tcatccaagc
    36241 cgaccaaacc gccatgaacg acctcgccgt caatatgtgg atgatcacga aatactggtt
    36301 cgacttcgac agctccctgc gcggtcgtac caagctgacc gaagactcca aagcacgcgg
    36361 catccgccgc accttaagcc tccttcgtcc ttatcttttg cccgaacacc gcaagaaata
    36421 cgaccggaaa atcggcaacg gcaacccgta aacccacagc gtccgaatca gccttcagac
    36481 ggcacttacc cgacggtatc agcgaatgcg cgcagtcccc ccgccccata tctccatcac
    36541 aggcctaggt tacctcggcc tgccgctggc acaaaagttt taccaacacg gcagccgcgt
    36601 cgccgccata aaacgcagcc tgacttcgga cgacatcaac ctgcccatac acctcgatac
    36661 catcgacctc aatcaagaca gcgcgtttca aagcgcgaac cttgcccgag atacaagctt
    36721 ttggcggcac catgccaaca aacccgtttg gttctgcctc ttgccgccat catcgctgac
    36781 acattacgcc gataccgtca aacaatgggc agaacttgcc cgggcgtgca acgtgcaaca
    36841 cctgattttc acaagcagta ccagcgttta cggcgataca gcgcgcgaat gcgacgaaat
    36901 cgccctaccc gatccgcaaa ccgagtccgc ccgccaaatc ctcgccgccg aacaacacct
    36961 gctcgacagc ggcgttccga acatcgacat cctgcggctg ggcgggcttt attgcgccga
    37021 acgccatccc gtcagccgcc ttgttcaaaa gcaaaacatc ccgggcggca accgccccat
    37081 caacatcgtc caccgtaata tcgccgtcga aagcctgttt cagacggcat ttaaccccag
    37141 cggcaggcgg ctgaaaaaca ttatcgaacc gcgccacccg acacgccgcg aattttttac
    37201 ggaagaagcc gccaaactcg gcctgcccgc gccggatttc gcacccgacg acagcgtggg
    37261 caaaatcatc cgtaccgttt gcgataacgg cttaagcctg taaaataagc ggcaacagca
    37321 acaaacaggc attctccgcc ccaatcagaa aatatgtccg ccttcctccc catcatcaac
    37381 cgcctgattc tgcaaagccc ggacagccgc tcggaacttg ccgcctttgc aggcaaaaca
    37441 ctgaccctga acattgccgg gctgaaactg gcgggacgca tcacggaaga cggtttgctc
    37501 tcggcgggaa acggctttgc agacaccgaa attaccttcc gcaacagcgc ggtacagaaa
    37561 atcctccaag gcggcgaacc cggggcgggc gacatcgggc tcgaaggcga cctcatcctc
    37621 ggcatcgcgg tactgtccct gctcggcagc ctgcgttccc gcgcatcgga cgaattggca
    37681 cggattttcg gcacgcaggc agacatcggc agccgtgccg ccgacatcgg acacggcatc
    37741 aaacaaatcg gcaggaacat cgccgaacaa atcggcagat tttcccgcga acccgagtcc
    37801 gcaaacatcg gcaacgaagc ccttgccgac tgcctcgacg aaataagcag actgcgcgac
    37861 ggcgtggaac gcctcaacga acgcctcgac cggctcgaac gcgacatttg gatagactaa
    37921 ccttcagacg gcatccgaca tgaacagccc ttttcacaac atcggcatcg taacccgccc
    37981 caacacgccc gacatccaag acaccgcaca cacgctgatt acctttttga agcagcacgg
    38041 ctttaccgtc tatctcgacg aagtcggcat aaaggaaggc tgcatctata cccaagacac
    38101 cgtcggctgc catatcgtca acaagaccga actggggcaa tactgcgacc tggtcgccgt
    38161 tttaggcgga gacggcacct ttctctccgt cgcccgcgaa atcgccctgc gcgccgttcc
    38221 gattatcggc atcaaccaag ggcatttggg cttcctgacc caaattcccc gcgaatatat
    38281 gacggacaag ctattgcccg ttttagaagg gaaatacctt gccgaagagc gcatcctgat
    38341 tgaggccgca ctcatccgcg aaggcaaaac cgccgaacgc gccatcgccc tcaacgatgc
    38401 cgtcctctcc cgtggcggcg ctggacagat gattgagttt gaagtcttcg tcaatcggga
    38461 attcgtctat acccagcgtt cggacgggct gattgtctcc acccccaccg gatcgaccgc
    38521 ctattcgctt gccgccggcg gccccatcat gcaggcagga ttacacgcct tcacactcgt
    38581 acccatctgc ccgcaatcca tgaccaaccg ccccatcgcc attcccgaca cgtctgaaat
    38641 cgaaatcctc gttacccaag gcggcgacgc gcgcgtccat ttcgacggtc aaacccatat
    38701 cgacgtgcaa aacctcgacc gcatcaccat ccgccgctac cgaaatccct tacgcatttt
    38761 gcacccgacc gactaccaat atttcaaaac cctgcgccaa aaactgcact ggggcgaaca
    38821 attggtctga accgaccctt accggaaaac acctatgaat ccaaaaactc cccccgacca
    38881 tacagaagat gccgtgcgcc tctccaaacg catggcgcaa ctggggcttt gttcgcgccg
    38941 cgaagccgac ggctacatcg aacagggctg ggtaacggtc aacggcaaaa ccgccgtact
    39001 cggtcagaaa gtttcaccgg cagaccgtat cgaactgaac aagaaagccc acgaacagca
    39061 ggcggcacgc attaccatcc tgttgaacaa acccgtcggc tatgtcagcg cacaagcgga
    39121 aaaaggctat aaatccgccg ccgaactgat tacccctgaa aatcactggg aaggcgatac
    39181 cggccgcatc cgtttcgatc cgaaacacaa aatcggcctc gcccccgccg gcaggctgga
    39241 catcgactcg gtcggattgc tggtattgac tcaggacggc cgtatcgcca agcagcttat
    39301 cggcgaaaac agcggcagtg aaaaagaata tttggtgcgc gtgcgcggca aattggacga
    39361 aaaaggactt gccttactga atcacggatt gagtttggac ggcgagaaac tgcgtcccgc
    39421 ccaagtagaa tggcaaaacg aagaccaact gcgtttcgta ttgaaacaag gtaaaaagcg
    39481 gcaaatccgc cgtatgtgcg aactggtcgg actgcgcgtc gtcgggctga aacgcatccg
    39541 catgggtaag gtcaagctcg gcaggctgcc gcccggcaaa tggcgttatc ttgctcccgg
    39601 cgaatcgttt taaataagca ttaaaaaatg ccgtctgaac gtccttacgt tttcagacgg
    39661 cattgcctta tgcccaaata acggtttctt ccaagggcct gcgcgctttc gtggcgattt
    39721 cctgaacgcg gtagccgaat gtcgcggcaa cggacacgcc ccattctgcc gcatcgaaca
    39781 aaccgaactg cccggacaat acgcgttcca tatcggcata gttgaaacct tccaccgggc
    39841 aggaatcgat acctgccatc gccgcacccg tcatcatgtt gcccaacgcg atataggtct
    39901 ggcggcaaca ccagtcaaac aaggcgcgcg aatcgtccaa aatcttgatg tcgtcggctt
    39961 gaaacgcctg atacctcgcc aaagattttg ccatggcatc cggttcggta acgccgcgcc
    40021 gtttgaggct ttccaacata aacgggctgt cgaagcgggc atttttcttt gccaaaaaca
    40081 ccaccaaatg actggcggta tccaaagcat ccgccatacc ccaagaaaac ggcttgattg
    40141 cctgtcggat ttcggggttt tgaaccacaa caaactgcca aggctccgaa ccgaccgaac
    40201 tgggcgacaa acgcccgagt tctaaaataa actgaaaatc ctcggcactg attttacgtg
    40261 ccgcatcgta atgccggcat gatttgcggt ttttaaatgc ggatagaacc tgctctttgc
    40321 ttaatactgc catcgcacat cctttcatta cttgtattgc aaattgattt caaaccgata
    40381 cggcactgcc gcaccataca atccgtcctc gccatcaagc ttcatccgga ctttacccat
    40441 ctctctacgg ctgggaaaac aaagcggcgg cagactcggg aataacgccc caatgccttt
    40501 cagacggcat ttgggcatca ttgcaaacgc gcctacgggt aggcgatata ggcataagcc
    40561 gaatggttgt ggatagactc gaaattctcg ctctcgacaa cgaaactctt gatgcgtttg
    40621 tcggcgatca gcgaagtagc gacatcgcgc accatatctt ccacgaattt cgggttttcg
    40681 taggcctttt cggtaacgta tttttcatcg gggcgtttga gcaggccgta gagttggcag
    40741 ctcgcctgcg cctccacata atcgatgact tcctcgatac cgacttcggc atcggcagtc
    40801 aggctgacgg taacgtgcga acgctgatta tgcgcgccgt attgggaaat ttctttggaa
    40861 cacgggcaaa gcgaggttac gggaatcatg accttcatac tgtggccgta tgccccgtct
    40921 ttgatttcgc ccgtgagaca gacatcataa tccagtaagg accgaatacc ggaaaccggc
    40981 gcggttttct tgcggaaaaa cggaaaagaa acgctgattt tgccggcgcg ggaatctaaa
    41041 agcgcgacca tttcggtagt cagcttgcgc aattgtgcaa aatccaaggc ttcggcatgt
    41101 tgctccatca atgcgacaaa acgcgacata tgcgtcccct tctgctcagc aggcaggtaa
    41161 accgtcatgg tcagacgggc aatggtggac tggatgcctt ctgcagtttg cagggtaatc
    41221 ggaaagcgca ggtctttgat accgacctga ttgatcggca gattgcgcaa atctcggctg
    41281 gattgcacgt ctgcaatagt gttcatgagt tgtttttcct taatattcat gtaattgttg
    41341 agatgtgtgc atggtatggc gtttccgccg gggaatatac cgtcaatctg caaatgcgag
    41401 attatatcac tcgcttttag cagctgcatt gattgtttct atttttcaga tagtgataaa
    41461 atcttcgatt attcacatct acataggcag agaccaaatg aaattcgcca cgaaagccat
    41521 tcattccagc tacgattgcg acgaacacaa ccgcgcgctg atgccgccga tttatcaaaa
    41581 cagtatgttt gcgttgcacg agattggcga aaatgtgcct taccgttatt cgcgcctgag
    41641 caacccgacc cgtcagattt tagaagacac cgttgccgat ttggaacacg gtgcggcagg
    41701 ttttgcgttt tccagcggta tggcgggaat tgatgccgta tggcgcactt tcctgcgccc
    41761 gggcgatacc attgtcgccg tcgccgatat ttacggcggc gcttatgatt tattggtcga
    41821 tgtttatcaa aaatgggggg tgaacgttgt ttttgccgat ttaggcaatc cggataattt
    41881 ggacgaactg cttaaagcgc acaaggtcaa actggtttgg ctggaaacgc cgtccaatcc
    41941 acttttacgc ttggtagaca tcaaagtcct tgccgcaaaa gccaaagcag ccggtgcgct
    42001 ggtcggtatc gacaacactt ttgccacgcc gtatctgcaa cagccgttgg atatgggttg
    42061 cgattttgta ttccattccg ctaccaaata tttgtgcggc cattccgacg tgttgatggg
    42121 catcgtcgtt gccaaaacca aagaactggc gcagcctttg cacgatatga tggtgcatac
    42181 cggcgcggtt gccggcccgc tggactgctg gctggtgttg cgcggcatca aaacgcttgc
    42241 cctgcgtatg gaggcacact gcaaaaacgc actcgaaatc gcgcgccgtc tggaagacca
    42301 tcctgccatt gaaaaagtgt tctatcccgg cctgccgtcg cacgaacatt atgaactcgc
    42361 ccaagcgcaa atgcctaaag gcatcggcgg cgtggtgacg gtttatctca aaaacgacac
    42421 gcgcgaagcg gcaaacagcg tgattaaaaa catgaaactg gtcaaaatgg cttccagcct
    42481 cggcggcgtg gaaagtttgg tcaaccattg ctattcccag tcccacagcg gcgtgccgca
    42541 taatgtgaaa atggaaatgg gcatcaaagt cggcctgctg cgtttctcca tcggcatcga
    42601 agacgcggac gatatttgga acgatatttc cgccgcactc gatacgactt tgtaaaccgt
    42661 aaaaatgccg tctgaaacca tagtttcaga cggcatttca atattgcgaa tcaaatatca
    42721 acgcttcagc atcttggccg cctcaatggc ataataggtc aaaatcccat ccgcgcccgc
    42781 acgtttgaat gccagcaggc tttccaaaac cactttatcg ccatccagcc atccgttggc
    42841 aaccgccgcc tgtagcatcg catattcgcc cgaaacctga taggcataag tcggcacacc
    42901 gaactcgtcc tttacgcggc ggacaacgtc caaatacggc aaaccgggct tgaccattac
    42961 catatccgca ccttcctgaa tgtccaacgc cacttcgtgc aacgcctcat cggtatttgc
    43021 cggatccatc tggtaggtct ttttatctgc cttgcccaaa ttgcccgaac tgcctaccgc
    43081 atcacggaaa gggccgtaaa atgcagaagc atatttggcg gaatacgcca taatccgcgt
    43141 atggatatgc ccggcatcct ccaacgcctc gcgaatcgca ccgatacgcc cgtccatcat
    43201 atcggaaggg gcaaccacct gcgcgcccgc ttcagcgtgg cacaaagcct gcttgaccaa
    43261 aacctctacg gtttcatcgt tcatcacata accgttttcg tccgtcagcc cgtcctgacc
    43321 gtgcaccgta taaggatcga gcgcgacatc cgtcataatg cccagttcgg gaaacctctc
    43381 acgcaaggct cggacggctg tcggcaccag tccttcaggg ttatacgcct cctgcgcaca
    43441 ttctgtctta tttgtcgtaa cgacagggaa tagtgccagc atgggtattc ccaaatttga
    43501 cgcttcctcc gccgtaaaca gcagcctgtc caaactttga cgcttcacac ccggcataga
    43561 aggcacatcc tcctcgcgcg ccgacccctc caatacgaac accggataaa tcaaatcatc
    43621 ggcggtcagc gtgtgttcgc gcatcaggcg gcgtgaaaaa tcgtccctgc gcatacggcg
    43681 catacgcgaa gccggaacat tgcggtaagg aaactgcata agccctccaa tcatcctgtc
    43741 aacaattcaa accatacgga agccgccgca acttgcagac agcatcgggc ggcaatcaaa
    43801 atccgttttc accggaaagg gtttcggccc gccgaaatgc cgcgccgaag gacggcgtcc
    43861 cgcccattaa agaacaatag caaaacaaca gcttcaggct gtcagccttc gatattctta
    43921 cgccacaata ccaaaagttt cccgatatgc tgaacaggtt gcgcgtcaac cgcctcacat
    43981 aaggcagtgc agatttcgat acgttcggca cggtcgtcgc cgaatacgcg cactttaatc
    44041 agctcatgcg ccgtcaatgc cgcatcggtt tccttgatga ccgcgtccgt cagaccctgc
    44101 tgaccgacca tcacaacagg atggagatgg tgcgcgcgcg ctttcagttc caaaatttct
    44161 ttggtgttca atttggtatc cgtcattttc ctactgcttg aataaaaaga ataatgcggc
    44221 attgtacgcg atttggcacg acatcggcaa aattaacgta caatacgcgg tttccattcc
    44281 gacccgcgaa ccactatggc tgtacgttcc aaatcctcaa aagcgtggct gcacgaacac
    44341 gtcaacgacc actacgtcca tatggcgcaa aaagacggct accgcgcccg tgccgcatac
    44401 aaacttttgg aaatcaacga aaaagacaaa ttaatcaaac ccggcacggt acttgccgac
    44461 ttgggcagcg caccgggaag ctggtcgcag gttgccgcca agctgacggg tacttccgga
    44521 gcagttttcg ccttggacat cctgcctatg gaagccatag ggggcgtctc cttcattcag
    44581 ggcgacttcc gcgagaacga cgtactggca caattcgaaa ccttgttgga caaccgcccg
    44641 ctcgaccttg taatttgcga tatggcgccc aatatgtcgg gaaacgccgt aagcgatcag
    44701 gcacgcagct tttatctgtg cgaactggct ttagacttcg cctcgcaaca cctgaaaacc
    44761 ggcggcagct ttttggtcaa agtctttcag ggtgcaggct atcaggaata catggcagcc
    44821 atgcgcgaaa ttttcggcac ggtgcagacg cgcaaacccg aagcctcgcg caatcgctcc
    44881 agtgagattt atttattggg caaaaataaa cgctgacaat acagacggcg tgctttacaa
    44941 tcatttccgt tttacacctc aattatggag ccttgctaag tggggaacac ctttaaatca
    45001 atccttgtct gggtcgcctt gggtatcggc ctgatggctg cgttcaacgc tttagacggt
    45061 aaaaaagaag acaacgggca aatcgaatat tctcagttca tccaacaggt caacaacggc
    45121 gaagtatccg gcgtcaacat cgaaggatcc gtcgtcagcg gctacctgat taagggcgag
    45181 cgcaccgaca aaagcacctt cttcaccaac gcgcctttgg acgacaacct gattaaaaca
    45241 ctgctcgaca aaaacgtccg tgtaaaagta acgccggaag aaaaaccgag cgcgctggct
    45301 gccctgtttt acagcctgct gcccgtcctg ctgctgattg gcgcgtggtt ctactttatg
    45361 cgtatgcaga cgggcggcgg cggaaaaggc ggcgcattct cattcggcaa aagccgcgca
    45421 cgcctgctgg acaaagatgc caacaaagtg acctttgccg atgtcgccgg ctgcgacgaa
    45481 gccaaagaag aagtgcagga aatcgtcgat tacctcaaag caccgaaccg ctatcaaagc
    45541 ttgggcgggc gcgtgccgcg cggcatcctg ctggcgggca gcccgggtac gggtaagacg
    45601 cttttggcaa aagccattgc aggcgaagcc ggcgtgccgt tcttcagcat ttcaggttcc
    45661 gactttgtcg aaatgttcgt cggtgtcggt gcgagccgcg tccgcgatat gttcgagcag
    45721 gcgaagaaaa acgccccctg catcatcttt atcgacgaga ttgacgcagt cggccgccaa
    45781 cgcggcgcag gtttgggcgg cggcaatgat gagcgcgagc aaacattaaa ccaattgttg
    45841 gttgaaatgg acggttttga gagcaatcag actgtaattg tgattgcggc aaccaaccgc
    45901 cccgacgtac tcgatcctgc gctgcaacgc cccggccgtt tcgaccgcca agtcgtcgtc
    45961 cccctgccgg acatccgggg gcgcgaacag attttgaacg tccattctaa aaaagtgcct
    46021 ttggacgaat ctgtggattt attgtccctc gcgcgcggca cgccgggttt ttccggtgcg
    46081 gatttggcaa acctggttaa cgaagccgcc ctgtttgccg gccgccgcaa caaagtcaaa
    46141 gtcgatcaaa gcgattttga agacgcaaaa gacaaaatct atatgggtcc ggaacgccgc
    46201 agtatggtga tgcacgaaga cgaaaaacgt gcgacggcgt atcatgagtc cggacacgcg
    46261 attgttgccg aaagcctgcc ctttaccgac cccgtccaca aagtaaccat tatgccgcgc
    46321 ggacgtgcgc tgggtctgac ttggcagctt ccggagcgcg accgcatcag tatgtataaa
    46381 gatcagatgt tgagccagct ctccatcctg ttcggcggac ggattgccga agacatcttc
    46441 gtcggacgca tctccaccgg cgcatcaaac gactttgaac gcgcaaccca aatggcgcgc
    46501 gaaatggtaa cgcgctacgg catgagcgac aaaatgggcg tgatggttta tgcggaaaac
    46561 gaaggcgaag tcttcttagg acgcagcgta acccgttcgc aaaacatttc cgagaaaacc
    46621 cagcaggaca tcgatgcgga aatccgccgg attttggacg agcagtatca ggttgcctac
    46681 aaaatcctcg atgaaaaccg cgacaagatg gaaaccatgt gcaaagccct gatggaatgg
    46741 gaaaccatcg accgcgatca ggtactggaa atcatggcgg gcaaacaacc cagtccgccc
    46801 aaggattaca gccacaacct gcgcgagaat gcggacgcgg cggaagataa cgcgccgcac
    46861 gctccgactc gggaagaaac cgaagcacct gccccggcag acaccgcttc gacagagtcc
    46921 gagcagcagc ctgaaaacaa ggcttaactt cccgaacaaa cggcagcccg caagctgccg
    46981 ttttcccatc ctgttttcag acggcatttt ccgctcaatg ccgtctgaaa cgccaaagcc
    47041 gtatcggttt ttgcggatct gcgatatggc ggtttaaatt gaaatcagac gaggcaaggc
    47101 tgtaccggtt taaaattaaa ccgctataat accgcccttc gaatacacgc ccgcattggt
    47161 gatgcctgtg aaaaaagttg aaaaaaacat cttggtgctg cacggcgcgg acaaaatgtt
    47221 cgagctggtc gataaggttg aagactatcc gcactttctg ccgtggtaca gcaagaccga
    47281 ggtcatcggg cgtagcggca acgaactgaa ggcgcggctg tttatggact atatgcacgt
    47341 tcgccaatca ttcgccacgc acaaccgcaa cattccgggc agggaaatcc gtatggaact
    47401 gctcgagggt ccgttcaaaa ccttacgtgg aacgtggaaa tttatcgatt tgggcgacga
    47461 tatgtgcaaa atcgaattca atttggaata cgatttttcc aatgccgttt tgtccgcctt
    47521 aatttccccc gtcttcaacc acctttccgt cacgctggtc gaagcgttcg tcaaagaggc
    47581 agaccgccgt tatgcttgaa attgagattg tgtacgggct gcccgaccga caggttttga
    47641 aaaccatgca gcttgccgaa ggaacaaccg tccgcgccgc cgcactgcaa agcggtttgg
    47701 acggcatatt tgaagattta aacctgcatt ccgcgccttt gggcattttc ggcaaagccg
    47761 tcaaagacga cacgccgctg cgcgacggcg accgcatcga agtgtaccgc ccgctgttga
    47821 tcgaccccaa agaagcgcgc cgcaaacgcg ttcaaaatca agaagaataa ccatgccgtc
    47881 tgaagccttc agacagcatt cgacagaaac acggaaaata tcatgtcaaa cacaatcaaa
    47941 atggttgtcg gcttgggcaa cccgggcaaa gaatacgaac agacacgcca caatgcgggt
    48001 ttttggttcc tcgacgaact ggcgtggaaa tggaaggctt catttaaaga agaaaaaaaa
    48061 ttcttcggcg aagtcgcccg tgccgccctg cccgacggcg acgtttggct gctcaaacct
    48121 gccacgttca tgaaccgttc cggacaggca gttgccgccc ttgcgcagtt ttataaaatc
    48181 aaacccgaag aaatcctcgt cgtccacgac gaactcgaca ttccctgcgg acggatcaaa
    48241 ttcaaactcg gcggcggcaa cggcggacac aacggcttga aagacattca ggcaaaactc
    48301 ggcacggcag actattaccg tctgcgcctc ggcatcggcc acccgggcga ccgcaacctc
    48361 gtcgtcggct atgtcctgaa caaacccagt acggaacacc gccgacagat tgacgatgcc
    48421 gtcgccaaat ccctgcaggc catacccgac atccttgccg gcaaatggga agaggcaacc
    48481 cgcttcctgc acagcaaatg acccgatgcc gtctgaagcc ctttcagacg gcatgttccc
    48541 gatttccata tccgaacagt catgaccgaa ctcaagcagc ttatccaaac cgaatccatc
    48601 cccgtcatcg aagaaaccct cgatttcctg ctgtacgaat gcagcatcga cgacgcacca
    48661 tccgccgaag aagtggcaca atggcgcgac atacttgccg cacgcggcgg caaattcctg
    48721 cgcctgtcca aaatctgcca aacgtggctg gacgaggagg cggcatgaag ctgccgcgca
    48781 accgcttcag cctgctttcc gcattgtggt ttgccggcgg catctattcg ctgctcttca
    48841 aagctgccga caccgcgccg ccgccgtttc cgcatttcga caaagcagca caccttgccc
    48901 tgtttttcgc acaaatctgg cttttgacca aagcattcaa aaccggaaaa cttcccatcc
    48961 cctaccgcag cctgatggtc tttgccctct gtttcgccct cttcagcgaa tgcgcgcagg
    49021 catggtttac cgcaacgaga accggcagtt tgggcgatgt tcttgccgat atggcaggta
    49081 cggttctcgc actctttgcc gcccgcgccg ccgaccgccc ggactgaatc ggtttatttc
    49141 ccccaaacag gatgcacctc ttcccgatac atcctatttt tccccttaca tatcaatccc
    49201 atctcataaa caaaaccgac aaatcgttta caatatattt acactacatc agattacaaa
    49261 tatactcgaa ccagttgcag aagcggcgat tccacaaacc gtttcggatt gcacgacaca
    49321 aaagaaaaac cgattttgtt gccttaaagg agtattcatg aacctgcatg caaaggacaa
    49381 aacccagcat cccgaaaacg tcgagctgct cagtgcgcag aagccgatta ccgactttaa
    49441 gggcctgctg accaccatta tttccgccgt cgtctgtttc ggcatttacc acatcctgcc
    49501 ttacagcccc gatgccaata aaggtatcgc gttgctgatt ttcgttgccg cactttggtt
    49561 taccgaagcc gtccacatta ccgtaaccgc actgatggtg ccgattctcg ccgtcgtact
    49621 cggtttcccc gacatggaca tcaaaaaggc gatggctgat ttttccaacc cgattatcta
    49681 catttttttc ggcggcttcg cgcttgccac cgccctgcat atgcagcggc tggaccgtaa
    49741 aatcgccgtc gccctgctgc ggctgtcgcg cggcagtatg aaggccgccg tgctgatgtt
    49801 gttcctcgtt accgcctttc tgtccatgtg gatcagcaac accgccaccg ccgcgatgat
    49861 gctgcctctg gcaatgggta tgctgagcca cctcgaccgg gaaaaagaac gcaaaaccta
    49921 cgtcttcgtc ctgctcggca tcgcctattg cgccagcatc ggcggcttgg gcacgctcgt
    49981 cggctcgccg cccaacctga ttgccgccaa agccctaaat ctggacttcg tcggctggat
    50041 gaagctcggc ctgccgatga tgctgttgat tctgcccttg atgctgctct ccctgtacgt
    50101 catcctcaaa cctaatttga acgaacgcgt ggaaatcaaa gccgaatcca tcccttggac
    50161 gctgcaccgc gtgatcgcgc tgttgatttt ccttgccaca gccgccgcgt ggatattcag
    50221 ctccaaaatc aaaaccgcct tcggcatttc caatcccgac accgttatcg ccctgagtgc
    50281 cgccgtcgcc gtcgtcgtct tcggcgtggc gcaatggaag gaagtcgccc gcaataccga
    50341 ctggggcgtg ttgatgctct tcggcggcgg catcagcctg agcacgctgt tgaaaacatc
    50401 cggcgcgtcc gaagccttgg gacagcaggt tgccgccacc ttttccggcg cgcccgcatt
    50461 tttggtgata ctcatcgtcg ccgccttcat tatttttctg accgagttca ccagcaacac
    50521 cgcctccgcc gcattgcttg taccgatttt ctccggcatc gctatgcaga tggggctgcc
    50581 cgaacaagtc ttggtattcg tcatcggcat cggcgcatct tgtgccttca tgctgccggt
    50641 tgccacaccg cctaacgcga ttgtgttcgg cacgggctta atcaagcaac gcgaaatgat
    50701 gaatgtcggc atactgctga acatcctctg cgtagtattg gttgctctgt gggcttatgc
    50761 tgtactgatg taaaccatcg acctaaacaa caagaccgtc tgaaagaata tttttcagac
    50821 gaccttgaag tttgtcgttc agacacaatt tgtcgaatca ttcaaaaccg gattctaacg
    50881 aaaggaaacc catgattatc ctgcacacca acaaaggcga catcaaaatc gaactcgatt
    50941 tcgacaaagc ccctgttacc gccaaaaact tcgagcaata cgtcaaagac ggcttctacg
    51001 acggcgtaat cttccaccgc gtcatcaaag gcttcatgat tcaaggcggc ggcatggatg
    51061 aaaacatgaa cgaaaaagaa acccgcgatc cgattcaaaa cgaagcgtcc aacggcctgc
    51121 ccaacgataa atacaccatc gccatggcac gcacttccga cccccattcc gccagcgcgc
    51181 aattcttcat caacactgcc gacaacgctt tcctgaactt ccgttctaaa gagctgtacg
    51241 gcaaaaccgt cgtccaagac tggggctatg ccgtattcgg taaagtcgtt gacggttttg
    51301 acgttgtcga tgccatcgaa ggcgtttcta ccaaacgtca tggttaccac gacgacgtac
    51361 cgagcgaacc tgtcgtcatc attaaagccg aagcggtata aaccgacaat ccgaaagcag
    51421 cctgcacaaa ggctgctttt atttcagacg gcatgttatt tgatgtaacc gtgctatgcc
    51481 gtctgaaaac cggacgcaag ccttcagacg gcataccgct atgttaaaat atgcccgttt
    51541 ttcctaaacg gacacaaagg aaaccttatg gcaaatattg cccgcgacat cttcaaagcc
    51601 tacgacatcc gaggcattgt cggcaaaacc ctgaccgacg atgccgccta tctcatcggc
    51661 agggccatcg ccgccaaagc cgccgaaaaa ggcatgaccc gcatcgcgct cggacgcgac
    51721 ggacgcttga gcggtcccga actgatggag cacatccaac gcggctttac cgacagcggc
    51781 atcgacgtcc tcaacgtcgg tatggtcgcc acgcccatgc tctattttgc cgccatcaac
    51841 gaatgcggcg gcagcggcgt gatgattacc ggcagccaca atccgcccga ttacaatggt
    51901 ttcaaaatga tgctcggcgg cgacacgctc gcaggcgaag ccattcaaga acttttagcc
    51961 attattgaaa aagacggttt tgttgccgcc gacaaacaag gcaacgtaac cgaaaaagac
    52021 atctccggcg aataccacaa ccacatcgtc ggccacatca aactcaaacg ccccatgaaa
    52081 atcgccatcg acgcaggcaa cggcgtaggc ggcgcattcg caggcaaact ctacaaaggc
    52141 ttgggcaatg aagttaccga acttttctgc gaagtggacg gcaacttccc taatcaccac
    52201 cccgaccctt ccaaaccgaa aaacctgcaa gacctcatcg tcgagctcaa aaacggcgag
    52261 gccgaaatcg gcttagcatt tgacggcgat gccgaccgct tgggcgtggt taccaaagac
    52321 ggcaacatca tctatccgga ccgccaactg atgcttttcg cgcaagacgt gttgagccgc
    52381 aatcctaaag ccaaagtgat tttcgacgtc aaatccaccc gtctgcttgc gccttggatt
    52441 aaagaacacg gcggcgaacc tgtgatggaa aaaaccggcc acagctttat caaatccgcc
    52501 atgaaaaaaa ccggcgcgcc ggttgccggc gaaatgagcg gacacatctt cttcaaagaa
    52561 cgctggttcg gcttcgacga cggtctgtac gccggcgcac gcctcttgga aatcctgtct
    52621 gcctccgata atccgtccga agtgttaaac aacctgccgc aaagcatttc cacgcccgaa
    52681 ctcaacatcg ccctgcccga aggcagcaac ggccatcagg ttatcgacga actcgccgcc
    52741 aaagccgaat ttgaaggcgc aaccgaaatc atcaccatcg acggtctgcg cgttgaattt
    52801 cccgacggct tcggtctgat gcgtgcttcc aataccacgc cgattctggt gttgcgtttt
    52861 gaggcggata cgcaagaagc catcgagcgg attcaaaacc aattcaaagc cgtcatcgaa
    52921 agcaatccga atctaatctg gcctctgtaa acacaggaaa aatgcttaat caaatcttgg
    52981 tagattggat ttggcttaaa aatctccgcg tccgttcgtg tttggggtgg tcgaacaact
    53041 ctttcgggct gccctgctct acgataacgc cgccgtccat cacgacaacg gtcgtggcaa
    53101 cttccagcgc gaacttgatt tcgtgggtaa cgacgaccat cgtccaacct tcccgcgcca
    53161 attccttcat ggcgttcaac acgtcttgca ccaactcggg gtcaagcgcg gaagtgggtt
    53221 cgtcaaacaa catcagctcg ggctgaatcg ccaatgctcg ggcaatgccg acgcgctgct
    53281 gctgaccgcc ggaaagctgg tagggataca aatccacttt gtcgcccaag ccgacttttt
    53341 ccagcagttt cagagcctct tcgcgcgctt gggcggcagg cttgccctgt acggcaaccg
    53401 gtccttccat cacgttttcc aaggcggttt tgtgcggaaa gaggttgtat tgttgaaaca
    53461 ccatgcctga tttgcggcgc agtgccaaaa tatcgtgttt gcttggtttt ttagaaaaat
    53521 cgattttcag cggtcgctcg ttgtcgaact cgatttgtcc gtcttcgggc atttccaacg
    53581 cgtttaggca tcgcagaaac gtcgttttac ccgagccgga aggcccgagg atgacgacca
    53641 cctgcccttt gcacacatcc aaattgatgc cgcgcaaaat ggtatttttg ccgaaggtct
    53701 tatggatatt gcggatttta atcatgacaa ctccttattt ggcgacatag cggtcgaagc
    53761 gtttttccaa gcgcgcctga atcaggaaca gcactttgca aaaacaccag taaaccaaag
    53821 cggcttcgat atagacgggc aaaaagtcat aagtgcggtt tgccgtttcc tgcgcgacgc
    53881 ggaataattc cgttaccgtc acgactgccg cgagcgaggt gtttttaaac aaaccgataa
    53941 actcgttgct caaaggcggc acggcaacgc gaaatgcctg cggcgcgacg atgcggcgga
    54001 acgtctgcat ataggtcatg ccgatggaga aacctgcttc ccattggcct ttcggtacgg
    54061 acaaaattgc cgcgcgtatg gtttcggaag catatgcgcc gacattgagc gaaaagccga
    54121 tgatggcggc aggaatcggg tcgatataga tgccgacgga aggcagcccg taaaacacaa
    54181 tcacaagctg aaccaacagc ggcgtaccgc gaatgacgga aatataaaat tccaccaatt
    54241 tcagcaggat tttccgcacg atgccgccgg cgggcataat ccgcaccaaa gccacggcta
    54301 ccgcaatcat cataccgata acgaaagaag ctgccgccaa aggcagagag accgcgaagc
    54361 cggctttgac cataggcaaa aacgcgctga caatcatatc ggcgcgtgtt tccgtcataa
    54421 acggcagcga agcaaggaaa ttattgaaca ctgatgtctt ttccgaagaa ttgttcgccc
    54481 agttttttca gcgtaccgtc ggctttcagc tcgttgattg ccgtactgaa tttcgccacg
    54541 gcttcgtcat tgcccttgtt gacaatcagg ccggaaccga ctttttcatc ggcaggtgcg
    54601 gaccaaacga ttttcacgcc cgcattcggg tttttcttca gatagtccaa aaccgccaat
    54661 tcgtcgttca gggttgcatc ggcacgtttt tgttcaatca gggtcagcga ttgcgccaaa
    54721 ccgtcaacag ccaccaaatc tgcgcctgca gctttggctt tttcgccgta gttgctggtc
    54781 agggattgtg cggttttcac gcctttgatg tcgtcgatag atttgatgtt gctgtcgtta
    54841 cgggcaacca atacggcacc gctccagctg taaggatcgg atttgtcgaa tgtcgcttgg
    54901 cgttcggggc tggtcagacc gacttggttt gccaccacgt cgaaacgccc cgccttcaaa
    54961 cccgccatca tcgaatccca ttgcgtttct ttaaactcga ctttcacgcc cagtttttcc
    55021 gccacggcgc gggttacttc cacatcgtaa ccggtcagtt tgccgtcctt gtcgtggtag
    55081 gtaaacggtg cgtaagtgcc ttccgtgccg acggtaaccg tgcctttatt gttgatgcgc
    55141 tcgattaaag aaccggaaac tgccgattgt gcaggcgcgg aagatgctcc gctgccgctt
    55201 tccgaaccgc cgcaggccgc caaaaccaat gcggcaatgc cgccgagtac gaattttttc
    55261 aacatcattc tctcctgtaa aaagtgtgca agtgcggcat tctaagtgaa attgcggcaa
    55321 acacaaagaa tacttttttt atatggttat tccaaatcgg aatggaaaag atatgccgaa
    55381 gccgccggaa gcggaaataa agggggcggg aaaggcgacg ggcggcacga aaccgaaaag
    55441 cggcatcagg tatgcaaacg gcggattcac gcacgccgtt ccggcaaaaa tccgcctgcc
    55501 gattcggtgc tataatccat ccgaaacaga tgacaccaaa aaggaacatc tcatgaacac
    55561 gccccaatcc gccatcattc ccgaccacgc ccaagccggc atctttatcg aagccgactt
    55621 cgccgccaac cgcctcaacg atattaaagc cgcctgccgc gcttcgctcg acgcgttatc
    55681 cgccttgaaa acccgctttc cggacgatat tttgggtctg accatcgcct tcggcagcaa
    55741 agcctgggca acattcggac atacggacga aggcagtgaa atcaaaccct tctccgaaat
    55801 gggcaacggg cttgcgccgt ccacgcagca cgatatgtcc atccacatcc aatctttccg
    55861 ccaaaacgcc gcctacgcgc ttgcccaatc cgttttgggc gcgttcggcg acagcatatg
    55921 cgtcgcgtcc gaagaacacg gtttgcgcct gtatcaggat cgcgggctgg acggtttcgt
    55981 cgacggcacg gaaaacccgc agggcgatga aaatgtccgc gaagtcgcca tcatccccga
    56041 aggaaaaccc gatgcgggcg gcagctatgt cctgctgcaa aaatacctgc acgatttgaa
    56101 aaaatgggat gccgtccccg tcgccgaaca ggaggcctcg gtcggacgcg gcaaggaaac
    56161 cgacgacgaa ttcggccacg atgtccgcct gcccgattcg cacctcggcc gcgtcaacct
    56221 gaaagaaaac ggagtcggcc tgaaaatcgt ccgccgcagc ctgcccttcg gcaaaatcag
    56281 cggcgaacac ggtttgatgt ttaccgcata ctgccgcacg ctgcacaata tcgaagcgca
    56341 attattgagt atgttcggcg acacggatgg caaaaccgac ctgctgctcc ggcacctctc
    56401 cgccgccgtt tcgggcggat actactacgc gccgtctgtc gaacgcctgc aaaacctcta
    56461 agccagacga tgccgtctga aaccattttt cagacggcat ttt
//
LOCUS       DAWFBO010000002        35727 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFBO010000002 DAWFBO010000000
VERSION     DAWFBO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.002"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(127..1989)
                     /gene="hscA"
                     /locus_tag="WGO87_000056"
     CDS             complement(127..1989)
                     /gene="hscA"
                     /locus_tag="WGO87_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0391495.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2160..3032
                     /gene="hpnD"
                     /locus_tag="WGO87_000057"
     CDS             2160..3032
                     /gene="hpnD"
                     /locus_tag="WGO87_000057"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0391496.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3111..4424
                     /locus_tag="WGO87_000058"
     CDS             3111..4424
                     /locus_tag="WGO87_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0391497.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPYYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            4508..5227
                     /locus_tag="WGO87_000059"
     CDS             4508..5227
                     /locus_tag="WGO87_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0391498.1"
                     /translation="MATLTDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            5392..6063
                     /locus_tag="WGO87_000060"
     CDS             5392..6063
                     /locus_tag="WGO87_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0391499.1"
                     /translation="MKTVSTAVVLAAAAVSLPGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            6073..6441
                     /locus_tag="WGO87_000061"
     CDS             6073..6441
                     /locus_tag="WGO87_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0391500.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGDNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            6438..7085
                     /locus_tag="WGO87_000062"
     CDS             6438..7085
                     /locus_tag="WGO87_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0391501.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            7374..8517
                     /locus_tag="WGO87_000063"
                     /pseudo
     CDS             7374..8517
                     /locus_tag="WGO87_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(8627..8836)
                     /locus_tag="WGO87_000064"
     CDS             complement(8627..8836)
                     /locus_tag="WGO87_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391502.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(8833..9129)
                     /locus_tag="WGO87_000065"
     CDS             complement(8833..9129)
                     /locus_tag="WGO87_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ0391503.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(9108..9713)
                     /locus_tag="WGO87_000066"
     CDS             complement(9108..9713)
                     /locus_tag="WGO87_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391504.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNAIETAPGLDALEKEIEEWTLNIG"
     gene            9793..10080
                     /locus_tag="WGO87_000067"
     CDS             9793..10080
                     /locus_tag="WGO87_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ0391505.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            10052..10354
                     /locus_tag="WGO87_000068"
     CDS             10052..10354
                     /locus_tag="WGO87_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ0391506.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(11076..13049)
                     /locus_tag="WGO87_000069"
     CDS             complement(11076..13049)
                     /locus_tag="WGO87_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0391507.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGAAADNAHIVFGDNTLRLHGANNRISYNNHDIFHKANKPRFAEDIKGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTQPGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPAGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(13060..13620)
                     /locus_tag="WGO87_000070"
     CDS             complement(13060..13620)
                     /locus_tag="WGO87_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0391508.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPSSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(13617..14672)
                     /locus_tag="WGO87_000071"
     CDS             complement(13617..14672)
                     /locus_tag="WGO87_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0391509.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLRRRNPTTASGTLTVSGIAQSMLSDG
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADELGAAANVRDGEAQLMAAPAGVSTECR
                     LTVQGGTDRESDASLLARLLEIIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLG
                     TVDIAITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGID
                     LDDAKRRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVN
                     RIEWFKAGAINVTEMPS"
     gene            complement(14686..15033)
                     /locus_tag="WGO87_000072"
     CDS             complement(14686..15033)
                     /locus_tag="WGO87_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ0391510.1"
                     /translation="MDKELNPGTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            complement(15133..15801)
                     /locus_tag="WGO87_000073"
     CDS             complement(15133..15801)
                     /locus_tag="WGO87_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ0391511.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDFEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(15798..16943)
                     /locus_tag="WGO87_000074"
     CDS             complement(15798..16943)
                     /locus_tag="WGO87_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0391512.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(16933..18264)
                     /locus_tag="WGO87_000075"
     CDS             complement(16933..18264)
                     /locus_tag="WGO87_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ0391513.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAVAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAAINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDMARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(18264..20435)
                     /locus_tag="WGO87_000076"
     CDS             complement(18264..20435)
                     /locus_tag="WGO87_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ0391514.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPDTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALGWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            20590..21172
                     /locus_tag="WGO87_000077"
                     /pseudo
     CDS             20590..21172
                     /locus_tag="WGO87_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21216..21485)
                     /locus_tag="WGO87_000078"
     CDS             complement(21216..21485)
                     /locus_tag="WGO87_000078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391515.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(21425..21790)
                     /locus_tag="WGO87_000079"
     CDS             complement(21425..21790)
                     /locus_tag="WGO87_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391516.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(21794..22171)
                     /locus_tag="WGO87_000080"
     CDS             complement(21794..22171)
                     /locus_tag="WGO87_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0391517.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(22236..23645)
                     /locus_tag="WGO87_000081"
     CDS             complement(22236..23645)
                     /locus_tag="WGO87_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ0391518.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(23648..23845)
                     /locus_tag="WGO87_000082"
     CDS             complement(23648..23845)
                     /locus_tag="WGO87_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ0391519.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(23842..24492)
                     /locus_tag="WGO87_000083"
     CDS             complement(23842..24492)
                     /locus_tag="WGO87_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ0391520.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(24482..24904)
                     /locus_tag="WGO87_000084"
     CDS             complement(24482..24904)
                     /locus_tag="WGO87_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ0391521.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(24904..25302)
                     /locus_tag="WGO87_000085"
     CDS             complement(24904..25302)
                     /locus_tag="WGO87_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391522.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(25367..26275)
                     /locus_tag="WGO87_000086"
     CDS             complement(25367..26275)
                     /locus_tag="WGO87_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ0391523.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(26275..27336)
                     /locus_tag="WGO87_000087"
     CDS             complement(26275..27336)
                     /locus_tag="WGO87_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391524.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            complement(27567..27983)
                     /locus_tag="WGO87_000088"
     CDS             complement(27567..27983)
                     /locus_tag="WGO87_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0391525.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(28111..29436)
                     /locus_tag="WGO87_000089"
     CDS             complement(28111..29436)
                     /locus_tag="WGO87_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ0391526.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(29423..30991)
                     /locus_tag="WGO87_000090"
     CDS             complement(29423..30991)
                     /locus_tag="WGO87_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0391527.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(30991..32613)
                     /gene="terL"
                     /locus_tag="WGO87_000091"
     CDS             complement(30991..32613)
                     /gene="terL"
                     /locus_tag="WGO87_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0391528.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFGNEIKSKWSR"
     gene            complement(32632..33441)
                     /gene="dcm"
                     /locus_tag="WGO87_000092"
     CDS             complement(32632..33441)
                     /gene="dcm"
                     /locus_tag="WGO87_000092"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0391529.1"
                     /translation="MDKQKPTVGSLFAGIGGFDLGFEQAGFDTAWQVEINPVPRAVLT
                     DRFPYAKQFEDVRTVLSELWPVDVIIGGFPCQDVSIAGKRKGLAGERTGLFYDAMRIV
                     DQLKPRWIVLENVTGLLNSNNGEDFQTVIKSLAECGYVGYWRVLNAAYFGVPTARRRV
                     FLVAGLGEYPPPMSLWLTPAQLESYLARLTRAGCKSRMVLCLEGFPMEPLTAQAQIFS
                     LRPTDGVRWLSGKERLTMMGFSSEWMRPTLRRLKLRETPSVRRSHAGLPKN"
     gene            complement(33434..33940)
                     /locus_tag="WGO87_000093"
     CDS             complement(33434..33940)
                     /locus_tag="WGO87_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0391530.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRASYTLADGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(33943..34140)
                     /locus_tag="WGO87_000094"
     CDS             complement(33943..34140)
                     /locus_tag="WGO87_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391531.1"
                     /translation="MSAPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(34137..34403)
                     /locus_tag="WGO87_000095"
     CDS             complement(34137..34403)
                     /locus_tag="WGO87_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391532.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(34415..34753)
                     /locus_tag="WGO87_000096"
     CDS             complement(34415..34753)
                     /locus_tag="WGO87_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391533.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYNTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(34750..34968)
                     /locus_tag="WGO87_000097"
     CDS             complement(34750..34968)
                     /locus_tag="WGO87_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391534.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            34981..35151
                     /locus_tag="WGO87_000098"
     CDS             34981..35151
                     /locus_tag="WGO87_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0391535.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(35196..35501)
                     /locus_tag="WGO87_000099"
     CDS             complement(35196..35501)
                     /locus_tag="WGO87_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391536.1"
                     /translation="MSLLKTVKMQAAVVLTALALTACSSTSAPSATEIKVVEKAVLPT
                     PPAALMVAPVRPNPPKDGKTVTLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(35398..>35727)
                     /locus_tag="WGO87_000100"
     CDS             complement(35398..>35727)
                     /locus_tag="WGO87_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391537.1"
                     /translation="GYKSAFAKQQAVIDKMEKDKAQALLLSAQNYARELEQARAEAKK
                     YEVKAHAVGMALAKKQAEVSRLKTENKKEIENVLTQDRKNAGGGCIDGFGSHGLQLYK
                     RALGYGN"
BASE COUNT         7406 a        10142 c         9470 g         8709 t
ORIGIN      
        1 acgcccttga tgacggtttt catcgaatag acaatgcctg acttttccat atcgatgttt
       61 ttcaagtgtt ttcgagcctt cagacggcat caggttatat ctatgccgtc tgaaaccgtt
      121 taagtatcaa atattatcga cactctggcc tgtcaatgcg cgttggatat tgcggttcat
      181 gcgtttggcg gcgaagttgt cggtgctgcg gctgagtttg gcgacggcgg agcggatgtc
      241 ttcggctttt ccgtctttca gacggccttg caaatcggcg atgccttgcc ggatctgttg
      301 caattctttg gcatccagta aatcgctgtc caactcgagg gcggcgttga cggcatcggt
      361 caggctttcg gcttcgacta cggcttcggc gcgggcgcgt gccgccatat cttcggcggc
      421 gttgtccata ctgtctttaa gcattcgggt aatcgcgccg tcgtccaagc cgtaggaagg
      481 tttgacttcg atttgcgcct gtacgccggt gctttgttct tgggcggaaa cggacagcag
      541 cccgtccgca tcgatttgga aggttacgcg gatacgcgcc gcacccgccg ccatcggtgg
      601 aatgccgcgc agggtgaatt tggcaaggct gcggcagtca gaaacgagct cgcgctcgcc
      661 ttgcacgacg tgtatcgtca tcgcggtttg accgtctttg aaggtggtaa agtcctgcgc
      721 gcgcgcggtg gggatggtgg aattgcgcgg gatgattttt tccgccaagc cgccgtaggt
      781 ttccaaaccg agcgacaaag gtgtaacgtc cagcagcagc cattcgccgt cggttttgtt
      841 gcctgcgagg acgtttgcct gtatggcggc gccgagcgcg acgacttcgt cgggattgag
      901 gttgttcagc ggggtttgtc cgaagaaggt ggcgactgcc tgttggacgt gcagcatacg
      961 ggtcgaaccg ccgaccataa tcacgccttt gacttcgttt ttaccgacac cggcatcttt
     1021 caacgcctgt gtgaccggtt cgagcgtttt catcaccaaa tgctgcgtca ggttgtggaa
     1081 ctcggcgcga ctgatgcttg tgtcgattgc catgccgtct gaaagcgtcg cctgaatgcg
     1141 cgcttcggtt tgcgtggtta attgttcttt ggcggcgcgg acgagcgaga gcaggagttg
     1201 gctgtcttgt tcgttgagtt gggagagtcc gttttgttcg agcaggcggc agaacaggcg
     1261 gtggtcgaaa tcgtcgccgc ccaacgcgct gttgccgccg gtggctttga cttcaaacag
     1321 tcctttggtc agttgcaata cggatacgtc gaatgtgccg ccccctaagt cgtacacgac
     1381 aaacgtgcct tccgaggcgt tgtccagccc gtaggcgatt gcggcagcgg tgggttcgtt
     1441 gagcaggcgc aatacgttca aacccgccag acgcgcggca tctttggtgg cttggcgttg
     1501 ggcatcatcg aaataggcag ggacggtaat caccacgccg accaaatcgc cgcccaaggt
     1561 ttcttcggcg cgcgatttaa gggttttgag gatttccgcc gacacttcga caggcgtttt
     1621 caccccctgc cgcgtatgca gttcgataac gcgttgattg tcgccgaaac ggtaaggcag
     1681 gtaatgcgta ttttgatgca gatcggcaag cgtccgcccg ataaggcgtt tggcggagct
     1741 gacggtgttc agcgggtcgg ttttttgggc ggacagggcg gttttgccga cttcaatgcc
     1801 gccgttttcc agatagcgga cgacggaagg cagggtaacg cgcccttcgg catcgggcag
     1861 acaggcggca ctgccgctgc ggacggtggc gaccaagctg ttggtcgtac ccaaatcgat
     1921 gcctgccgcc aaacggtgcc ggtgcggggc ggcggacata cccggttctg aaatctgcaa
     1981 aagagccata aattctgcct gtcggtgcgg tgtgtaaagg aaggaaagcg gcattttaac
     2041 agaatttgcg ccgatagttg agcaaaccgc taaggattgg gtcgtgccgc cgaaattgcc
     2101 ctctaccgcc aaatgccgat aaaatccgcc tttcccgccc atcattagga atataaatcg
     2161 tgaaaggact cgactattgc cgccaaaaag cagaagaaag ccgctccagt tttttgtcgg
     2221 gcttccgttt cctgactcag gaaaaacggg atgcggtaac ggttttatat gctttttgcc
     2281 gcgaattgga cgatgtggtt gacgaatgtt ccaaccccga tgttgcacag gcgacattga
     2341 actggtggcg cggcgatttg gacaaggtat tcggcggcgc gatgccggaa caccccgtca
     2401 atcaggcctt gcggcaggtt aaggaaacct tcaagctgcc gaaatatgaa ctggaagcct
     2461 taatcgacgg gatgcagatg gatttggttc gggcgcgtta cggcagtttt gaagaattga
     2521 aactgtactg ccgccgtgtt gcaggtgtag tcggctgcct gattgcgcgg attttggggt
     2581 tttcagacgg caaaacgctg gaatacgccg acaagatggg acttgcgctg caactgacca
     2641 acatcatccg cgatgtcggc gaagacgcgc gcaggggacg gatttacctg ccgatggagg
     2701 aaatgcagcg gtttgatgtc cccgcaagcg tgattttgca atgcagcccg acgggcaatt
     2761 ttgccgaatt gatggcgttc caaatcaaac gcgcccgtga aacctaccgc gaagccgtat
     2821 cgctgctgcc ggctgccgat aaaaaagccc aaaaagtcgg actggttatg gcggcggttt
     2881 attacgagct attgaacgaa atcgaccggg acggcgcgca aaacgtcctc aaatacaaaa
     2941 tcgccctccc ttcgccgcgc aaaaaacgca ttgccctgaa aacctggtta ttcggattca
     3001 aaccgcgccc cggcacgccg gaacgggcat aaggcgcata ccgcccgccc ttcaggaaaa
     3061 cccaaccccc gccgccccga aaaacggggt ttcaaccatt ggaggaataa atgatgaaca
     3121 cgccgcatcc gcgcccgaaa atcgccgtca tcggcgcagg ctgggcagga ctgtccgccg
     3181 ccgtcacctt ggcgcggcac gccgacgtta ccctgtttga agccggccgg caggcgggcg
     3241 gcagggcgcg cacactggcc ggaaataccg acggtttcgg ttttttggac aacgggcagc
     3301 acattttgct cggcgcatac cggggcgtgt tgcgcctgat gaaaaccatc ggttcggatc
     3361 cccgtgccgc ctttttgcgc gtaccgctgc actggcatat gcacggcggt ttgcagttcc
     3421 gcgccctccc cctgcccgcg ccgctgcata ttttgggcgg cgtgctgctt gcccggcgtg
     3481 caccgactgc attcaaagcc aaactgcttg ccgatatgtc cgatttgcag aagtccgcac
     3541 gcctcggaca gcccgacacg acagttgcac aatggctgaa acagcggaac gtgccgcgtg
     3601 ccgccgtgat gcagttttgg cagccgctgg tgtggggtgc gctcaacacg cctttggaaa
     3661 ccgcaagcct gcgcgtgttg tgcaacgttt tgtccgacgg cgtgctgacg aaaaaatccg
     3721 gcagcgacta tctcctaccc aagcaggatt tgggcgcaat cgtcgccgaa cccgccttgg
     3781 cggagcttca acggctcggc gcggacatcc gcctcgaaac gcgcgtatgc cgtctgaacc
     3841 ccctcccgga cgggaaagtg ctcgtcaacg gcgaagcttt cgatgccgcc gtccccgcca
     3901 ccgcgcccta ccacgccgcc gcgctcctgc ccgaaggcac gcccgaacac gttcagacgg
     3961 catatcaaaa ccttcgctat cacgccatca ccaccgtcta tctgcgctac gccgaacccg
     4021 tccgcctgcc cgccccgctg accggccttg ccgacggcac ggtgcaatgg ctgctttgcc
     4081 ggggcaggct cggactgcct gaaaacgaag tgtccgccgt catcagcgtt tccgaccgcg
     4141 tcggcgcgtt tgcaaaccgg gcgtgggcgg acaaagccca cgccgacctc aaacgcatcc
     4201 ttccgcattt gggcgaaccc gaagccgtgc gcgtcatcac cgaaaaacgc gccacaaccg
     4261 cagccgatgc cccaccaccg gatttgtcgt ggctgcaccg gcaccgcatc ttccccgccg
     4321 gcgactacct ccacccatac taccccgcca cgctcgaagc cgccgtacaa tcaggtttcg
     4381 cttccgcgga agcctgcctg caaagcctga acgatgccgt ctgaaaacgc cggccggcat
     4441 caggacgctt tacaaggtgc ggcaaaacgc taaaataccc ccgaccgacc accaaaaagg
     4501 aaaaagtatg gcgacactga ccgacaaaac catcttagtt accggcgcat cgcaagggct
     4561 gggcgaacag gttgccaaag cctatgcggc agcaggcgcg actgtgattt tggttgcccg
     4621 tcatcagaaa aaactggaaa aagtgtatga cgcgattgtc gaagccggat accccgaacc
     4681 attcgccatc tgctttgacc ttattagcgc ggaagaaaaa gaatttgaac atttcgccgc
     4741 caccattgcc gaagccacgc aaggcaaact ggacggcatc gtccactgcg ccggctattt
     4801 ttacgccctc tcgccgctgg atttccaaac cgtcgccgaa tgggtcaacc aataccgcat
     4861 caacaccgtc gcacctatgg ggctgacccg cgccctgttc ccgctgctga agcagtcgcc
     4921 cgacgcgtcc gtcatcttcg tcggcgaaag ccacggcgaa acacccaaag cctactgggg
     4981 cggcttcggc gcgtccaaag ccgcgttgaa ctacctgtgc aaagtcgccg ccgacgaatg
     5041 ggaacgcttc ggcaacctgc gcgccaacgt cctcgtcccc ggccccatca attccccgca
     5101 acgcatcaaa tcccatccgg gcgaagccaa aagcgaacgc aaaagctacg gggacgtgct
     5161 gcccgcattt gtctggtggg caagtgccga aagcaaaggg cggagtggcg aaatcgttta
     5221 cctctaaatc cggaaaaggc aggcatcccg cccgcgccgc gattccgttt gaaaaatatc
     5281 cgtaccttcc gcgcgcatcc gtcccgcaca gacaaacacc ttgacttaaa tttacataag
     5341 gacaataatg ataactcgtt atttccttaa cttgacctta gggtaaattt tatgaaaacc
     5401 gtttccaccg ccgttgtcct tgccgccgct gccgtttcac tgcccggctg tgcgaccgaa
     5461 tcctcacgca gtctcgaggt agcaaaagtc gcctcctaca atacgcaata tcacggtgtt
     5521 cgtaccccga tttccgtcgg aacattcgac aaccgctcca gcttccaaaa aggcattttc
     5581 tccgacgggg aagaccgttt gggcagccag gcaaaaacca ttctggtaac gcacctgcaa
     5641 cagaccaacc gcttcaacgt actgaaccgc accaatttga acgcattaaa acaggaatcc
     5701 ggcatttccg gcaaagcgca taacctgaaa ggcgcagatt atgtcgttac cggcgatgta
     5761 accgaattcg gacgcagaga tgtcggcgat catcagctct tcggcatttt gggtcgcggc
     5821 aaatcgcaaa tcgcctatgc aaaagtggct ctgaatatcg tcaacgtcaa tacttccgaa
     5881 atcgtctatt ccgcacaggg cgcgggcgaa tacgcacttt ccaaccgtga aatcatcggt
     5941 ttcggcggca cttccggcta cgatgcgact ttgaacggca aagttttaga cttggcaatc
     6001 cgcgaagccg tcaacagcct ggttcaggct gttgacaacg gcgcatggca acccaaccgt
     6061 taaggagaaa tgatgaatcc gaaaaccttg agccgtttgt cgctgtgtgc ggcggtcttg
     6121 gctctgaccg cctgcggcga caacgggcaa aaatccctgt attattacgg cggctatccc
     6181 gataccgtct atgaaggttt gaaaaacgac gacacttcgt tgggcaagca gaccgaaaag
     6241 atggaaaaat actttgtgga agccggcaac aaaaaaatga atgccgcccc gggtgcgcac
     6301 gcccatctgg gactgctgct ttcccgttcg ggagacaaag agggcgcgtt ccgccagttt
     6361 gaagaagaga aaaggctgtt tcccgaatcg ggcgtattta tggacttcct gatgaaaacc
     6421 ggtaaaggag gcaagcgatg aaacctttga ttttagggct tgccgccgtg ttggcgctgt
     6481 ctgcctgcca agttcaaaaa gctcccgatt tcgactacac gtcattcaag gaaagcaaac
     6541 cggcttcaat tttggtggtt ccgccgctga acgaatcgcc cgatgtcaac ggaacatggg
     6601 gtgtactggc ttcgaccgcc gcgccgcttt ccgaagccgg ctattacgtc ttccccgccg
     6661 cagtcgtgga ggaaaccttc aaacaaaacg gcttgaccaa tgccgccgat attcacgccg
     6721 tccggccgga aaaactgcat cagattttcg gcaatgatgc ggttttgtac attacggtta
     6781 ccgaatacgg cacttcatat caaattttag acagcgtgac gaccgtatcc gccaaagcac
     6841 ggctggtcga ttcccgcaac ggaaaagagt tgtggtcggg ttcggccagc atccgcgaag
     6901 gcagcaacaa cagcaacagc ggcctgttgg gggctttggt cagcgcagtg gtcaatcaga
     6961 ttgccaacag cctgaccgac cgcggttatc aggtttccaa aaccgccgca tacaacctgc
     7021 tgtcgcccta ttctcacaac ggcatcttga aaggtccgag attcgtcgaa gagcagccca
     7081 aataagcata tgccgtctga acctctccga gcttcagacg gcatatgcga atcttgatac
     7141 ttgcaagccg caccgcccaa aatcgggcgg ccggtttcaa tatccgaaca aggttcgggt
     7201 tctgtatttt gcagaacccg aaccttttat tcaaatttaa acttaattcc ggcgtggttg
     7261 tatggtaaat taacacgcaa tatgccctga cctgcaaaac ttccccaaac ttcatcaaaa
     7321 aatcaactgt acccgtcttt ttcagacggc attgataccg taagaaattt cccatgcaga
     7381 aatggcaaac cgagctttat tccacaccgc tttggctttg tctgacactg aaaagcgcgg
     7441 tcaaagtctt gctgctgatt acggcgatga ttacgctggt gttaaccgaa atgcggctga
     7501 atgtgttgag tacctttatg tccaaagggc tttacgactc gatgcaggat ttgaaatgct
     7561 tcggcatttt ggatgtttgc tgcaatgaac gcgggcgtgg tgctgatacg ggcgtttaac
     7621 aacgtcgtca acgacttcct cgatcaaggc ttggcgatta aatggtcgga gcggctcaac
     7681 gaagtgctga cagcacgctg gcttgccgac aaaaactact accgcctgca aatgcgccgc
     7741 cacgcgccag acaacatcga ccagcgtatc caacaggacg cgcaggaatt tatcgcttcg
     7801 accatcgaat ttgtgcgcgg catggtcaat tcggtcgtta cttctctgga atttgccgtt
     7861 gttttgcggg gcttggctgg catcctgacc gtgttcggct tcgacattcc gcacggcatc
     7921 gtttggtttg tctatatatt tgtgattttg gcgaccttta tcgccatgtg gataggcaac
     7981 cctttgattc gttacaatta tgaaaacgaa aaactcaacg gcgactaccg ttattccctc
     8041 atccgcgtgc gcgaccacgc cgaaagcgtg gcgttttaca acggcgaaaa acatgaatac
     8101 gaccagcttt ccgaccgctt taaagccatt atccgcaacc gttggcgcat tgcgcggcaa
     8161 agcgtctgct taagcggctt taacgatatg ttcaccaacg gcatcaaact cttcccgatt
     8221 attttgcaag ctccgcgcct gtttgccgga caaatcaaaa tcggcgacat ccagcaaacc
     8281 gtccaagcct tcgcgcgact gcaaaacgcg ctttctttct ttcgaatgtt ctacaacaaa
     8341 ttcaccgcct accgcgcccg attggagcgt ctgtacggct ttttgctgag taccgaagaa
     8401 caacacagcg cgcggcaacc cgagattagc gaagtttcag acggcattgc actggaatgt
     8461 aaggcggcgg cagcttttgc acgaattggc ggcagctttt gcaccgccga agcgtaggaa
     8521 attgtattag cttttgcaca aatttatccc cgtttaatgg tctgttaaac ggggattatc
     8581 ttatgccttt aaaagtagta cgctacttgc ggtcagaact tataaatcag gtggtcgtcg
     8641 tagcttgaat tgctgttgat gtgctgctca cagtcaaggt ttggctgcct gcaaaacgct
     8701 cccatccggt caggcgcagt tctgccacga ggtcaagata agtaggcagt tcgctgcggg
     8761 tgccggagaa gaagacaaat ggcggccgca ccattgccat caggcgcaga aactctacca
     8821 tgccgaagta gttcatcgcg tccccttttc cccggcggcg cggctttggt tgaaccgcgc
     8881 gtcgtgctgc ctgcctttca gttcgctgat atacgcctgc cggcagtggt ggcggtcgaa
     8941 aaatacgccg ttgatcaggg tgtataaaac cttccagcgg gttttgggct tttgggcgag
     9001 ccgcgcgccg cgataggtgc ggcttgagag ggtttcgtcc gccgcgccgc cggttagggc
     9061 gttgaacagt tggtcgatgg ctaccaacag gtggtaaacg tattttttca gccgatgttt
     9121 agcgtccatt cttcgatttc cttttccagc gcgtccaatc ctggcgcggt ttcgatggcg
     9181 ttcaatttgt cttcgagctg ctgacgcttt ccgataatcg cgccggcggc aacagccagg
     9241 cgggcggatt tttcgataac tttttcaatc aaaacgtcca attccacgcc ccttgcggcg
     9301 gcgatttgcg ccagcatcgg ggtcggggcg ttgttgtccg cctgccgcgc gagggcttct
     9361 ttttcctgcc tgtaaaagct gtcgatttcc acttggggat agcccgccaa gaggctgttt
     9421 ttgagttcgt ccgcctttgc cgcgaggcgg aatgccgtgg cggttttttg ttcggcgaaa
     9481 cgggcggcgg cagcggcttc gccgatttcc catttcttgc cgtcccattc gtggtattcg
     9541 gacgggcgcg gcggggttaa aacggggcgg ccgtcggaat ctgcggcaat ctgcccgccc
     9601 tgcgcctgtc ctgccaaaag ggcggcgtat tcttcggcgc ggacggcaac cgcgccttcg
     9661 gggatgctgc ccaaggtgtc gtcgtaaaag ccgtttttga aatagatggt catttcgggg
     9721 tttccttttt tgtatttaca ttttggtttt ttgccgctta taatgtaacg acaaaatacg
     9781 ctgcaagctg caatgaagat tgaatttgac tccgaaaaaa atcaacgcaa tatcgaagag
     9841 cggaatttgc cctttgaatc agtcgggcaa atacgctgga caacggccgt tattgtgccc
     9901 gatgtctgct ttgattaccc cgaaccgcgt tatgttgccg ctgcttatct aggcgatacg
     9961 caacgcctgc acattgtctg ttttacgccg ataaaagacg gcatccgtgt catcagtttt
    10021 cgcaaagcga ataaaagaga ggttaaaaaa tatgcaacgt caatccttaa caaacgctga
    10081 cggcgaagtg cgggaacttg ccgccgaaga ttttgcgctt gcccgcccca ttgccgaagc
    10141 cctgcccgaa gacttggcac aggtgctttt ttcccatcaa aaacaattgg aagaaaaggg
    10201 cgtaatgcaa aaacgaaaca caggcaaaac cccgaaacaa ctcgttacca tacgcctgtc
    10261 cgccgatgtg gtcgaaaaat tccgcgccgg cggcaagggc tggcagacgc gcatcaacga
    10321 ggttttgcgc caatatgtcg cccaattgaa ataacggggg cggccgctgc ttatcgggcg
    10381 cgcccgacta tcctttcccg cgcggtttcg gccgtgcggt tttttgttgc cggtgccgtc
    10441 cgaagggggc agcggctttt tgcgcggtca ttcccgcctt tcgttattcc cgccactttc
    10501 cgtcattccc gcgaaggcgg gaatcccgaa acccctgccg ccgtcattcc cgcgcaggcg
    10561 ggaatccagt ccgttcggtt tcagccgttt ccgatagatt cctgccgcgt ttggggtctg
    10621 gattcccgcc tgcgcgggaa tgacggcggc gggggtttct gttttttccg atagattcct
    10681 gttgcgtcgg ggtctggatt cccgctttcg cgggaatgac ggcggcgggg gtttctgttt
    10741 tttccgatag attcctgccg cgtttggggt ctagattccc gctttcgcgg gaatgacggt
    10801 ggcgggggtt tctgtttttt ccgatagatt cctgccgcgt ttaggggctg gattcccgct
    10861 ttcgcgggaa tgacgaaagc ggcgggaatg acgaagagtg gcaggattgg cggggcatag
    10921 gtttcggggg attggcggcg cggccggttt cggtcggatt gcttggatcg gcgggctgaa
    10981 gcccatctct acagcccatc tctacagccc atctctacag cccatctcta cagcccatct
    11041 ctacagcccg tctctacagc catctctaca gcccgctagt cttgcagtgc ctcgagcaag
    11101 ccgtctcggc ggcggcggtt tcggggtccg gttgatgttc ttccactctc tctttgcggt
    11161 tcttcgttgg tttcaggtat ttcggggcct atcggggagc tggcggcgtt tcccgaggcg
    11221 gctttgccga tggccatcca gtcgcaactg ccttcgtacc agttggtaat aatggcggcg
    11281 gcgtggtttt ttgcgcggat aaacagccag ttgtgttgcc ttcgatcgcc ggtaacgcgt
    11341 cccgaatggc gttcggatac gaagcatttg acgttgccgt cggcaaaggc gacggggaag
    11401 acgacttcgt tttcgatggg gccgccgctt cttgtaaacc tgtaactgcc ggtctgtatc
    11461 attgtgccgt cggggaatct gacaatatcc acgccgccga tggtttggcg tgtgaattgc
    11521 gcggcaacgg cggtgctgat tttttcgtcc agcccttgga tttgggcggc ggtgtgggtg
    11581 tggtcgcggt cggctttgtc ttgcaaacct tgcgccagcg tgcccgcgtc cagcgcgcct
    11641 gcgttgatgg tttcgccgtg ggactttatc caaaacacgg cttcgcccaa ggtgtcggcg
    11701 gctttgatgc acagttttaa aaccagggct ttggggcggt tttcgtcgcc gcccgttgcc
    11761 attttgctgt ccgagcgcgg ggttaaaaag ccgttgtcgt ttaaatcgcc gtccgtccaa
    11821 gtcgatacga gcgcgcttct ctgcctttcg ttgtggtctt cgtaaccgac ggctgccgtg
    11881 tctgtgtggt ttgtccagtg tgaaaatact ttgtggacgt gccgtttgat ttcgtcttcc
    11941 tgcttcgttc cgactgccaa gctgttgcct gcgttgcgga taaagcggtc ttccgcctgc
    12001 gggacgtttt ggatgctgcc gtattttccg gtcagcagac ggtacagctc gggataagcg
    12061 gtttcggtta cgcgcgtgcg gatgtcgtca aacgccagcc agccggtcgg gatttggtcg
    12121 gacggaaacc acgcggtgat gccgatgtcg gtacggctca aatcgggcag gcggttgctg
    12181 ttgcccaagg cgcggtagag gtcggggaag gtgttttgcg caaaggtcgt gccgttggct
    12241 ttgagatagc ctgcggggtt ttgtacggct ttgggaaagg atacgatcgc gccggcgggg
    12301 atgccgtccg aattgagttt tttccatgcg gaccagttgt cgtttgcgct gctcgtttgg
    12361 tggcgttcgt acacgtcgga cgtgtaggcg ggataaccca actgcctgca ccagccgggt
    12421 tgcgttccgg cgatgacttg gatatggcag gcggtgtttt caacgggcag gttggagctg
    12481 ccgaccgccg tcggcaggga atagatgccg tctgttttga gggtgttcaa atcgccccgg
    12541 aatgtttcga ctttgaaatt gccgatgccg tatccggaca gtgtgttcgg tttgcctttg
    12601 atgtcttcgg caaaacgcgg tttgttggct ttgtggaaga tgtcgtggtt gttgtaggaa
    12661 atgcggttgt ttgcgccgtg caggcggagg gtgttgtcgc cgaagacgat gtgggcgttg
    12721 tcggcagccg cgccgatgta ggctttgtgg ccgccgttcc agttcgtctg gttggcggat
    12781 aaatggatgc cgctttggaa ttgggtttgg gcagtaaagg ttttttggcc ggtgatggtt
    12841 tggtcgccgg tggtaactac agccagccta tttagtgctg ccagtaattg attaggctgc
    12901 gacttatccg gctggatacc ggccgcagtc agcacggcca gcagctcgga ttgcacttgg
    12961 ttgagccacc acgcgggcag gatggtgccc aattcggaca cgccgtcgcc gtcgtggaag
    13021 gtcttgtcgg gggtttcgat ggggtgcatg gtttagtcct tatcggtagg taaatcggat
    13081 agcggtgtgg gcgggcttga ggcggttgaa caggctctcg atcacggcat cgctgtaatc
    13141 ggtcagcctg tcgcccgccg ccgagatacc ggcgcggaat cgggtaatgc ggttgttgcc
    13201 gccgcgcacg ttaacgtgcc acacccacat gatttcctgc ggcgcaagac ggtcgccggc
    13261 gcggtttaca ccggcgcgga acggctgcgg ttcgtcgatt tggatttgat agcccgccgc
    13321 ctcggccaaa cgcacaaaat aaggaatact caagccgcct gtttcgttta gcttggccat
    13381 gacggccaac acacggcgct ggcggttttt gcccgtaccg tccaaaccta atacgcgctc
    13441 ccaatcggcc agcatttggc cggcgctgct cgggtcgacg gcatcggcaa cgctttgagc
    13501 cgattccgcc accgcatcca gcgctgcgcc gtctatttct gcctgcgccc gcacacgcgg
    13561 ggcattgcgg gcatacgaca cggggggcaa cagaccccgc aagatgtctt gatagctcat
    13621 gacggcatct ccgttacatt aatcgcgccc gctttaaacc actcgatgcg gttaaccgta
    13681 tcggcggcac ggttggccgt cggcgcagtc agacggcggt cgatcacacc atccacattg
    13741 ctgatagccg cctcgatttg cgacacagtc agaccgtcgc cggggatcag ggtgtcgaaa
    13801 tattctttta gagccgtccg tatgcggcgc ttggcatcgt ccaagtcgat gccgtcgagc
    13861 ttgacttgca cggtaacagg caccgccgtt acggttggct tgagtaccag cgcatttttt
    13921 gccgttaccg ggcgcatctc gtcgatataa gcctgtacgc ggcgcacagt ttcttccgac
    13981 ggcacaccgt cggcggaggt aatggcaata tccaccgtac ccaagccgcg gcgcagcgga
    14041 taaacatatg cgctggttac gccgtcaaca ctcaacgccc agtttttata gtcgtaacgg
    14101 ttgccgcctg cgggcggtcg gcggatgatt tccaacagac gcgccagcag tgaggcatcg
    14161 ctttctcggt cggtgccgcc ttgtacggta aggcggcatt cggtggacac accggcgggg
    14221 gcggccatca gttgcgcctc gccgtcgcgc acattggcgg ccgcgcccag ctcgtcggcg
    14281 attgccggta tttccgccgt gccgccgctg ccgataacgg cgcgggcggt agtgcggtaa
    14341 aaacgctggc cgatacgcac ttgcaggccg tctgaaagca tggattgcgc aataccgctt
    14401 acggtcagcg tgccgctggc cgtggtagga ttgcggcggc gcaagccgcg catggaggca
    14461 tgccgctcca aatactcgcg gtcggcggta tcagggaaaa tctgccgcac aatccagctt
    14521 tgatgcgcat attgcccttc ggcgcagctg gccaaacggc tggcgtgcac ataatggtcg
    14581 ctgtcggggc tgatatcggc atccggccac aggcttttgg tatcgcgcag gatacgctcg
    14641 cggatttgct caaatgtcgg cgtttcaaac acgtttaaaa cctctttaaa tcacgggcac
    14701 ttcgtggcgg taatcaaacc cgcccgccgc cgtatccacc cggatatgca ggatcaggcg
    14761 gccgtcgtgc ggctgctctg cgcgcacggt aatcttgtcg gcacgcccgc tcttaacaat
    14821 cggttgcagt gcctcatcgg catattgctc ggccaacagg ctgacccgtt gcaggtcttt
    14881 ttcgcgctgc aacaaatgca gcagcgagcc gagcgtttta tccgcccacc agctgcccaa
    14941 cggtgtcatc aaacggatat acacggcatt ttgcagcgta tcgacggtgc ggccggtata
    15001 gtcgccggtg ccggggttta gctctttgtc catgttggca ttttgccgtt ttcagacggc
    15061 ctcacggagt acacgggctt caatcccgca cgcacaaaaa agcagcccga aggctgccaa
    15121 aggtctgctt gtctatgccg gttccgccgg taaggttttg ccgccgatgc tgtcggtatg
    15181 cgggtgctgg cgcaacgata tattgccggc caccacgtcg ccgtcggtgt taaagctgcc
    15241 gcccgtttgg ttaacatcgc cgctaaaggt ggctccgtcg ccgccctcga cggccatgcc
    15301 gccgttgccg ttgatttggc cttgcgccgt caacactgca ctggtctcca ccaacggagc
    15361 gttaaatttg gcatccgtgg ccgcattaac ctcgtattgt ttgcagttaa cccggtacac
    15421 gtcgcaatcg gcctcaatga ttttgccttg cttaatcacg atttttgcac cctcatgatt
    15481 aaaaatcgcc gtctcgccgg gcttaaggtt tttgatgcgg tagctgccgt gctggctgca
    15541 cacaatcaca ccgtgcgaag tattgccgcc cagcggtatc actaccgctt cgctgccgtc
    15601 gggcggatgg ctggcaaagc cgtattcctg caaatgttca aagtcttgca gggtttcgtc
    15661 ggccaagccg ctcaactgca cgcgctgtat cggctcggac gacaccacca gcgtgatttt
    15721 tccccgaaag gccgcgcgca gggtttcgcc gatattttta gcagtttgtg ccgttttttt
    15781 cgccaattta ctcaaactca ttcaaacacc gccgtttctg cttgtttttt gccgcctttt
    15841 ttgcccttat ggctcacgcc tttgcgtttg cctttgcgct tgcgcgccgc ctcggccttt
    15901 ttggggtaag cgtcgggtgt ccaaataccg tcctctttga gccgcagctc ggtttgcgtg
    15961 ccatccatgc gggatagcat aaaccgccgc cccatcagaa aaaacaccgc atcgataccg
    16021 tgctcgtcgt cgatcacatg cacacgctgg ccaggttgcc acaatacgcc gtcgcgggtt
    16081 ttatggccgc ccacggttat cgtgagtgta aatccctcca gccgccagtc ggccagctgc
    16141 tttttagcct gcttttgcaa tgcggccaaa ttgtcggcat cggacaccac caccgtttta
    16201 gggcggtgca gcgtcatcgt cgggtctttg tacacccact ttaaatcgtg tttggcgctg
    16261 tcgccgctgc ggccgtgcga ttgcgccaaa aaagtaacct cggaaaagcg gttgtcggta
    16321 tcccactcaa tgtccatgcg ctcgatattg cagcggctgt cggtgcggct ccaacacaat
    16381 gtcgccaccg gcgggctgct gtaatccgca ccgcccacca ccaacgtgcc gtccggctcc
    16441 agccacggat gcagcccgac cgagttggca atatgggtta atgcctgcca tacggtttcg
    16501 cccggctcga tgtcgatttt gtccaaagcg gggttgtttt cggccttaag caccaccgct
    16561 ttaatctgcg gccacggcgc ggccagcttt ttggctgcat ccaataccgt catgcccttt
    16621 acattgagct gcggcgcgga gcaatccacc aaaaaaccgg ccaaatcacg cccgctcaag
    16681 ctcaactcgc ggctgccctt gcttttgccg tggcgctggc tgccgatgat gcccgtcatc
    16741 acgatttgcc cgtctatcac tacctcgcag ctctctccgc ttaaatcggg tatggccgcc
    16801 tccggcccca acctgccgat gacaaaatcg aagctgtcgg cagggattaa aaagtcgctg
    16861 tcgatgtcgt agcgctccca gtggcggtgc tctttaccgc ccacgcgcac cgacacggca
    16921 tagccgtatg aattattttg catagctgtt gaccaaagtg ccgcgcttga taaacgcggg
    16981 gtggtggata tggggattga gccgcaccag ctctgctgcg cgggccatat cgccgtaaaa
    17041 ctcgtgggcg atttggtgta tcgtaccgtc gattggggct tggcgcacaa tcagcggcgg
    17101 cttttggttg atggccgccg caaccaacgc attcagacgg cctgccgccg cgcgcaggga
    17161 ttctgccgtt tggtaagcct cggtatacac ggcgttggcc gtcagcccac cagactcggc
    17221 agcagccgtc tgcaccgccc gcaaggcggc aatctcggcc tgcatacggc ggcgcatggc
    17281 gcggttaacc tcaatcaaat cgggcgcggt catctcttcg ccatgcgcct cgattaatgc
    17341 cgttgccacc gacaacagcg atgacgtgga taacaggcgc accgcctgcg ctaccggctg
    17401 cacctgtttg gcggttaacc ggttcaggcg gttttgcagg ccgtctgaaa agcggccggt
    17461 cagtaaatta tcagggatag cggcggcacg gtcggcaaca gccacagccc cgtcaaaccg
    17521 ctgtcgcggc gaccaaccgg catggtgcat ggcattatcg gccaaacccg cctcacggcg
    17581 tatgccagta tctaccatga ccgatatatc cgcaaacagc ttggccgagc cgtttttaaa
    17641 cgccgctgcg ctgtatccgc cccgatcggg aaaggcaatt ttgtccaaat caaacaaacg
    17701 gcgcaccgca ccaaacgtac ccgatgccgc actccaaatg cccagcgcgc tgccccataa
    17761 agccgatacg cccgcatcca ccgccaacac cgcatcaaca aagccgatag ccgcctcgcg
    17821 gtaggtgtcg atatcagcaa tcaacgcctc aagctcgacc aaaaaggcgt tttcaaaaac
    17881 aaagatttcc tgcgcttcgg ccgcctcgcg gaaagtaata tcgatgcccg cataatccac
    17941 ataatcggcc tcatgtcggt aactccatga tgccgcaatc atgttgtgca tccgccccca
    18001 aacagggtgc accagcacgc cgccgcccgg ctgctccagc gcatccagca gctttttcag
    18061 acggcctgca tagcccttgc cccaaaacac cgcattaatc tgcacctgcc gcccggtcat
    18121 gcccatgtct tcaaggtcga taccctgcac aaacggccgc gcatgctcgg ccaatgcctt
    18181 gccgttgctc tcgtccacca cctcaatatc aaagccgacg cccttgtaag atgcgtcctg
    18241 caataaggta tgccatccgc tcattattga cccgctccac ggccgaacat ggccacttga
    18301 taccgcgata cctcattcgc gataacacgt ccgtcgatat tcaggctcac attggtattg
    18361 atggtctgac ttgccgacgc catcgcactt tgcacttgtg ccaaacctgt tgtaactgca
    18421 gccgtatcct gcgccaatgc tgcctgatac gccgccgtct gctgctgaat ggctgcctga
    18481 tacgccgccg tctgagttga aaacaacgga gacaacatct tatcggtagg ctgcgctgac
    18541 ggagcagcca aatactgagg gacaccaccc cgcgcctgtt cccgcttcgg cgcaggttca
    18601 aatcggatac cgttgttttt cagacgatta atcatttcag gagttgtctt ggtaccacgc
    18661 aaaccctttg gcaatgtgcc gtcacccaaa gactcggaat acagcaacat acctaacaaa
    18721 cccgcccgtt taactaacgc tggattattc atgagcccgc taccggcaga ttttccccat
    18781 gacagcaact taccgcctgc cgctgtggca cctgctgcca cgccgcctgc ggaagcctta
    18841 ccccatccca acgctttact acctacatct ttcagaaagc ctgcaccttt accgccaccg
    18901 gcggttaata acattgcggc agacgccgct gttgccgccg tcgtcaatgc ttgcagggct
    18961 agagtggcat tagggtattc cgctgccagc tttttaaacg aggtttccat atccaccaat
    19021 gttcccgtca gactttcccg caacattgcc agcgattcct gttgctcttg ttgcgccgct
    19081 gccgacaaca ttcgcgcctc gttgttctta gcaatttttc cgttatccaa cgttacgcca
    19141 gccaacgaag ccatatattc acggatttgc gtcatatccg ttgcagccag caaaccttgt
    19201 tttgcctgca aatcaggcag cagttgcgcc aaaagcgcgc ccttaagcat atttgcctgc
    19261 tccgccgccg tcttatcgcc tgcagccgcg cgtttcttat aatcttggta ttgcttatcc
    19321 tttactagca tggcatcggc aagacgggac aacacctgca ctgcgttttc gccgttttgc
    19381 ttgccttgca caaccgagcc tatccaatcg acacctttct tcgggtcatt cggatttgcc
    19441 atcttcttca gacgacctat cgtgtcaggc gacagagttt tactcaaaag attttgcaca
    19501 ttagtcgccg cttcggcagg actgcccgat ttattcgccg cagattgtaa gagtgagagc
    19561 aggtagtcca aaccgccgac accattcatc cctgcctgtt gcgcggcagg gagcaggctc
    19621 ggcagctccc gaaccatatc ccgcacctcg aaagtgccgt ctaaacccga ttgcaagacg
    19681 tgctcaagcc cgagttgcag gtctttaccg ctcatgccgc catctttcag ggttttaatc
    19741 agttttgccg tatcctcgcc actgccttct gaggcaagtg caaaagcata tgccgcctgc
    19801 gcttcattct tggtttgggc aaaattcaga ctggtggtca tcatgccgct gattaaatcc
    19861 aaagccttat cgtgggtccc gccatttttc tcgacaagtt caagtgccaa atccttgatc
    19921 tgttgcgcac cttcagttgc aatccacgct gccgatttac tgttatcctc aataaatgcc
    19981 tgtctggaca cgcggttgat gttctcatca agctgctttc tgttgtccat agcaggctta
    20041 agcaccgtat acgctgccgc gccaccagcc accgcagctg caccgaatct tccgattttt
    20101 cccatcttgt ccgcaaatcc cgtgccctgt ttcagttccg cgttcagctc gcgaattcgg
    20161 ttacgcgtag cgaccgccgc ccgtgccaaa tcattttgtg atgccttgcc gctgcgcgcc
    20221 aaacggttaa acgcagcctg tgtcagcatg atttcgcgtt gaatctgttt ttctgagcgg
    20281 atgccggtca acgcataagt atggctttgc cgttcaagtt gcgcccgcga tttcgcggta
    20341 cgatctaatt gtcgttgagt atcagccagt agccgtctcg ctccgtcatc tcgggcggtt
    20401 aacaccaacg acagtttcat gttcccgttt gccataaaaa gcagccttta aacgtattaa
    20461 ctcaaggtca attatcgttt aaaggctgtt taagggaggc ggtatgggct tcaaccccgt
    20521 tttcgtgctt tatttgtttg ttgcgttata ataccagaat gataaccatt ttattcaaag
    20581 ggatatttta tgaatatgaa aaaattgatt tccgcaattt gtgtttcaat tgttttatca
    20641 gcctgcaacc aacaatcaaa aacggcacaa gccgaagaac ctgtccaaag tatccaggct
    20701 gctgattgta ccgccccaat ggacatcaca gttgaacaat atctcatcaa tttggagcaa
    20761 gcatttaaaa ctcagaacgt ctcaacaaaa atccataata aaaatattgt caagaccgat
    20821 tgtggttatg accttacttt ggtaatggat tttggggcga ttgcgctcaa actggacgag
    20881 cagcaaaaaa ttagagctat ctcagtaggc tacattttaa aaaccgacgg agagaaagga
    20941 caaaatctag tcaataatgc cataaatgga ttacacagta ttcaggcagt tctgtcttta
    21001 actaccacag acaaattggg cgaatcggaa gcaggaaaac aacttcgtca aagaatccaa
    21061 tcagacagga gcaacagcgc aaaaagacgt tccggcagat ggtattttat atagcgttgt
    21121 ttttgaaaaa gaaacaaaca ccattgcaat aatcggcaga aaacaaccct aaccgtcaaa
    21181 taaaaaaaag gtcgtctgaa actcagacga cctttttatt ttccctccgg ctttctcagc
    21241 cgtttcgaca caatcactcc ctcttccttt ggcgttttta tgcccaggct cgacaggatg
    21301 tcttcaaacc atgccgacag ctcggcgtgg cacatatcgc gcactgcgtc cgcccctatc
    21361 cccgcctttg ccatcaggat gaccgcctga cggtagcttt tgtgtgccgt caggtagctg
    21421 cgttttactg ctccgccgcc gacggcgcgt cggggctcgc cgtagcggcg gacggtttta
    21481 ggcacaaatc ctcctgagcc gccatcacgg tatggtagtc ttcggtagag aggttttcca
    21541 tcagccactg cgccgtcagg atgtcggatg ggataccttg ggcttcaatc tgctgcgacc
    21601 agtaggtcag cgtctctagg acgtcgcgtt gcagcagttc ggcttttgtg gcgttttcgg
    21661 gcagctcggg caagtcatca atcagggcga acgccgccag ttcgccgccg acggtcagtg
    21721 gcttgaggcg taattcagat acgactttgc cattgtaggt cagcccgtgg gtcagtttga
    21781 cggtaatcac gcattactcc ttgattttgt gcaaagcaat catctcaatg tcgatgcgtg
    21841 cctcgttatc gacttcatat tgctcgctgg tatcgacggt aaagcagtcg aggtaggaag
    21901 tgcggcgacc ttcgtcgttg atggggtaaa tcgtgatttt cgccttggtg atgttgtccc
    21961 agtcgatttc cgtaccgtcg atcggaatgg cggcggtaac gcttaatttg tgttcggtta
    22021 cgccgtcaca ataaccgttg actcggccgt tgcggttcat cgttttgacc ggcttgcggc
    22081 ctgtagtggt ttgcggcttg atgctcacaa tctcgatatc gcggccgttc acctccataa
    22141 tcaccgcgcc ggtataggta gcgtcgctca tgctttatcc ttttttaagg tttcagacgg
    22201 cctttaaacc tgttttaaac gccgtctgaa aagggttaca aaatcaaatc aatgcgcccg
    22261 gcaaagacgt gcaggccgtt gaccacatcg gcggggataa tggcattaac acggttgggg
    22321 tcgttttgcg cacgcgccac caccagcttg cctttgttgg cctcggcgtt ttcgatgatt
    22381 tcggcttggt cgagcttaat cagcacgtcc aaaatctcgc tcttaacctt gggcagcagg
    22441 cggtcgctca atttgtcgcg cggaaaacgc agggcaatgc gctctttaac gctgcggcgc
    22501 acataatcca gcgtgcgaat ggtggtaatg tcgagtagtg ccgggtcgtc ggtgttgttg
    22561 gccgacttgg tataggttga tacggcacgc ataatctgca cgcggttgtt gaccgctgtg
    22621 agcggggtca agccgttgta cagcgcattg ttgcattctg caaaccacgg ccattgcgca
    22681 tcaggtgtaa cggccagccc tttgatttcc agcgtgttca gcggcttggc agggtcttct
    22741 tcaaaggcca acaccgccgc ataacctgcg gcgatgatgc cgtttggctc taccgcacct
    22801 ttgtaccaag cacaggtaat gcggccgtcg ttgattttgc cggtagcggt ggtggccgtg
    22861 ctcaaggccg cactcatacc caatacgccg atacagccgc gctgctcgat ggcgttgaat
    22921 acgttggtaa tatggttgct caaggctttg gcgttggcgt catcgctaaa cgggctgcaa
    22981 ataatgtgat aatgcttgcc cgccactttg gacaaggccg tggcaatgtc cgcatttttg
    23041 gcaccgccgg taaagacatt ggcttgataa gtcaggccgg tattgccggt gctcacggtt
    23101 aagccgctct cgttgccgat ctcgcctttg tgtttggccg tcagcgtaac ggctgcgccg
    23161 ctgccggatg cggtaacggt tacatcggcg gcagtgatgg cggttttcag acggcctgcc
    23221 actgtggcgg cggtctcgcc ggtgttaacg gccgtgctta cctgcttgcc gccaatcgtg
    23281 atttccacca cgcccggcgc ggtggccgtg ccggaaaggg taacggttgc ggttgcctgc
    23341 acgcctgcgc tgtggtcggc aataccgata acggtcaaat ccaaataagg gttgttggca
    23401 aatgcttggc gcaccatcaa atgcgccagc gagccttgtc cgaacaaatc ggccgcctcg
    23461 gcatcgctaa atagttgcac cggctctaag gcgggctgta tgcccgcggt cagcatgggt
    23521 gcaaccatca ataccttttg cggattttgc ggcaaacctt gcacggcatt gcgggtgtta
    23581 aattcaatat actgcccggg cacgcggatg ctgcccggaa tcgtgtcaaa atcaatatgg
    23641 ggcatcatta ctctccctta gtattgcggg ttttggtatt tggggcggca tcctcaacca
    23701 ccaccaaatc gccgtcatca atcatgcggc ggtaatacag gctgttgccg tccacctcca
    23761 ccggctcttg gccgatatat tcgtgcgggt tgtgttcggt cggcacacgc aggccatctg
    23821 ccgccgttac tttgattttg ctcattgctt atccttaagg gttaggttta caggtatttc
    23881 atcggcggat tgcgggtcgt aaattttgcc gtccaacccc tcgaaatcag gccacggctc
    23941 gctcaatgta ccctgatact tggtaaagat atggttgggg tcgtcgggat tgtcggtgcg
    24001 ttcggggtag cggtcatttt ccaacccgca ggtgttaaag cggatggcat actcaaccgc
    24061 atatatgctt actgcggcgt tttgcaccag cacatgattg gcaatcgcgc gcaccgcttt
    24121 gggcaccaag ccgcggctat cggcaaaacc gagccgctgg ccgtcaagca ggcggcgaac
    24181 agcgcggatt aaatcgttgc tgccgatttc gcggctgtcg atgccgcctt gccgctgcgc
    24241 ctgctcgttg cgcagattgc gggccgccac catcaccaca aattcggcgg tatcctgata
    24301 acgtccgcat acgccgccgg tgctggcagg ctcaactttg ctgccgccat acgttaccca
    24361 aaccgcaggc agcgtatgga tttgccccgc caaatcgtcg gcctcgccgt tgtagctttt
    24421 aaccgtgcgc accatgcgac ccaagccccg tttcagacgg tctgttatcg cttgctcaat
    24481 atcagtgatc acgcccgaac accttatttt taccgtttac aaacacaata ccgtcttcgc
    24541 ccgcggccac ttcttcgccg ctatcatcca cgcccagctg cacttcgcct ttggccatag
    24601 cacgcagcag gtcgagcacg tcgattttgt agcggttgcg gatttcgtcg gtaatcaaca
    24661 cgccctgagc cgccgtcaga cggtagcggg caatgtcgca gcaaaggcgc accaagatgg
    24721 gcggcagatc ctcaaaaggt cgtctgaacc gccccagata cgcgtcgatt tcggcagtgg
    24781 cgtccaccag cgcggtttgt gcgacctcgc ggtcaatcag cccctcgttg ttgcggtcgg
    24841 tgagctgcaa gacttccagc tcaccgaaac gcgcaaccat atcctcaacc gtcgcgtatg
    24901 ccattactcg accgccttgc gttgcagcat aggctcggcg cagattgcct tccacaccgc
    24961 ttcgccgact tcggcgcgct tcacttcacg ccagccgccg tcaaacagca ggccgccgcg
    25021 ccaaaattct ttgccgtctg cgccggtact gacgagcatc acatcgcggc tgtccgccaa
    25081 agcgtcggcg gcgcgttgcg tatgctgcac tttgagttcg gcaagttcgg cggacagtgc
    25141 ctttttgtcg tcttcggctt tttccaaggc tgtggtcagc atttcgacat cgtttcgggc
    25201 ggcggcaagc tctgcctgca cggcgtccaa ttcggctttg atgtcttcaa acgacggggc
    25261 ggcggtttcg gcggtttctg gtttgttgtt ggtttttgcc atgatgactc cttgtttcag
    25321 acggccttta aataggtgtt aaaggtcgtc tgaaaacggt tcggggttac aggtggacgc
    25381 aaacgtgcag tttcagacgg cctttgaagg tattggtcgt gccgttgatt ttgtcggctt
    25441 ccaataattc acgggctttg tcttccagct cgggcggaac gaccaagagg ctgggcttca
    25501 cattcaacac atagccaccg tcggctttga ttttctgcat tttgacgata atggcggcga
    25561 ggttttcgcg ggtcagcgcg gttttttccg ccatgtgcat cagttgccac aaaccgaaac
    25621 cggcattgca gcggcggcgc gatccgtaca ggtattcgtc ttccataaac acgcggtcgg
    25681 atttggacgg gtcgaatttg gtttcaaact cggagtcggt gcggttttgg aaaatcagcg
    25741 gcttcagggt cttggtgtca tcgacgacgt agaaggtcgg cgcgtcgttg tccgtaccgg
    25801 tcgtcagatt ggagtgcggc gtattctgac ccgtgccgtc cgatttctcg taaacggggt
    25861 ggtcggtatc gaagaaatat tggccgtcat aaccgacagt ggttttacct tttttgagca
    25921 gaccccacac cagcgtatcg ggcagggcgg cggcggactc gcccattgcc gccatcatcg
    25981 ggcggtacat gccgacttgg tcgtcttcga tgtccgtacg cgctacgccg acggttgctt
    26041 caaacttttt gttgtcgagg ctcatcgcct gcttgttcat tttttcgatt tggcgcgagc
    26101 cgacccattc gcgcatttgg gggaatttgc cgagccacgc ataagtattg gttgccgtat
    26161 tggacggaat ggtcatcgca acggcggtat agtcgggctt gacggattcc aaacccgttt
    26221 gaaattcttt gcggaatgct gccgtgattg cggtcaaaat cgctgatttg tccattactt
    26281 accttcgctt tctttgattt ttacaaattc ttcgccggac atgcccagca tttttgctgc
    26341 ggctgcctct tctgcagtca gtgcggcgac gcgttcgtcg ggtgctttac cgcccgtttg
    26401 cgagcctgcc agtgcagcga ccggctgggc gttttcaata aagccggtca aaaatgccaa
    26461 gccgcccggc tgtttcaata cgccttctgc ccactccttc tgagcaggca gcaatttgcc
    26521 tgaagtcagc gcggcggtaa tcaattcgtt gcctttgtct gcttcctgct tggcagtcag
    26581 cgcggcgact ttgctttgca gctcttgaac cactgagata ggcgcgtatt tagtcaaatc
    26641 aggcttggcg gtttgcgccg tcaatgccgc gatgcggctg tctttttccg ccagctgcgc
    26701 gaacacgtcg gcagacaatg ccacgtcttt cggcttggct tccacgagcg cggacaatgc
    26761 cgccttcagt tcttcttcgc ccgcatcagg cagaccgaac agttgctgta acaactcttt
    26821 catagggttt tgctccgttt ccggttttaa aatttgcgcc gacgctgccg ccagcacctc
    26881 gtccataccg tccaacgcgg ggaaatttgt cagcgcggcg tgaaaaattt tgcttacata
    26941 tccctttgtg tcataggaaa acacagcaga gatgtagcga tactcttttg cggcaattgc
    27001 cgcagccgcc ttgtccgtcc actccacttc ggcaaacatg cctttaggcg tgaactccag
    27061 ccaacgcatc caaccggcgg caggtgcagg ttgtccgttt ttctctttgt agagcgtctg
    27121 gtgttcataa tcgacaacca actgattgcg cgagctgttg gccaacaacg cgacatcatg
    27181 accgttttct tcggtcagat accacgcagg gacatcagtc ggacgaccgt ctactgcgcg
    27241 aaattcgcca tatggcagca gttggattcg cccgtctttt ggctgcacct cgaaactgca
    27301 cacggcaagt agggtttttt gtgcattttt ggacatgtgt acctctctca atacggtcat
    27361 tttgcctatt tgaaaaaaca acaaagagta ggcgtgcttc aaaccctccc gccatttttt
    27421 taaaacctgt ccgcgcgata cacaattaat cgttttcagg ggctttttag aagcgtctag
    27481 gattgatttt tatatcttgg gcaggggttt gtattacccg cataaaaaaa cgcgctaaaa
    27541 agcgcgtttt tgagggtttt ataaattcac ggtatcagac ccgaaaaata atcctgcaca
    27601 tcgtccatca aagcctgttt gtcgtcgtcc gtcagcgtca aaaattcccg ttgcggaatc
    27661 cgaactttgc ggttgcgccc cgccataccg ccgaagttgt ggatggcggc atagacgata
    27721 ttcgtaccga caagggcggt atcgttgtct gacagtgtgg aaaaactgtc tttcagacga
    27781 cccgaatccg aaagcggctt gccgtcgcgg tattttagcc ccaaccattt cggacggcct
    27841 gcgtagcgga aattgagctt gaccgccgtg tgcatggttt cggacagtcg gcgcatcagc
    27901 aggtagcggt tttcgatccc gttgccaagc cgctcgattt ggtttaggac gacaaagata
    27961 ttgtctattt tgacatcaat catggtatta tcctgcctag gctactaaaa aagcggtgaa
    28021 tttgtctgcc ctccgcagcc cttatgcagt cctcggactg ggattggtat ccgttacata
    28081 agcgagagtg tgtcgggatt gcagtccgac ttagtagcct tttttcattt catagcgttt
    28141 cttacttttc acaatttctt tttcatccgc cctgcgaaac gaatccaaaa aaatcgaatc
    28201 gtactgtggc agatatttca aaactgccat caatctgtat ttttcgttcc cctccaacgg
    28261 ttttccgagc aaaacaaaac tttcttttcc tctgcttttg tcataaaaaa tatattcggg
    28321 ctcatgaatc agatcgggga gcatggcata taaatctgtt ccaaacgatt gcccctcccg
    28381 actgtccacc tgtttgacca atgtatcatc agacagccac accgtcgctc gtgtcatacc
    28441 tgccaattct tgcgtttcct tgctcaatac acccgcagca aatttaagct gtcttgatag
    28501 cgcatttcgg attttgattt tctgctcttt atcgggcttg ccatcaatat ccaaacgttg
    28561 cttgacttca taaaactctt tttcaagctg tttaaagctg gttttaaaat ccgcaccacc
    28621 catttccgct ttggcaaatt gatgcgccaa cgcacggtcg tacttgtcta aatcggggcg
    28681 gtagttcatt cgtccggcgt tgtaatcaaa tcctcgatcg gtcgtgtaca gactgccatc
    28741 cggtgcttta taggcaaggg tcagataagt atcgcctttt ttgttgtaga ttttatgggt
    28801 ctcgactaga ttgtccgccg tgctttgccc gacaatccgc ccctggcgtt ccacatcccg
    28861 ctccgacagc gcgatgaccg agcaacggca gttgtagccg ttgggcgggt aaaacgttgc
    28921 ccaaaacggg tcgtcgtagc ggtacaccag cccgtctatt gccgaatgcg ccggacgggt
    28981 gcggctgtcg cctaccgcgt catacatcca ataaggccgt gcatcaatat ttgccatata
    29041 tccttgatat tgaccggcgt tgtaggcagt ttgcatgttg gtacggtaaa tcgtctccaa
    29101 cctccgcggc gaaccgaata cctcgccggt ggctgggtcg atgatatcct taccgttatg
    29161 cccgttcgga tgcagccagc ccttgttgcc gagcagattc aagatacctt ttcgccaatc
    29221 gtcaaacgac tgtccacttt ttgccgattc aaccatcgaa gttttgatgt cgttgagcat
    29281 atccaagtcg gtcatgcggg caatcgtata gactttggca atttcggagg ctgtcagatt
    29341 gcggtagctc tccgccgtaa cctttttact ttccagccac tcgattgcct tttttggcgg
    29401 cagacttaag gcaaatccca aatcaggcgc gggcatggtc ttgtcccaaa atatcgctga
    29461 taaacaaggc ctgctgcata taggtacgca gtttcgcgtt gtccaaattc ggataaagcg
    29521 cattcagtgc ggcatctgcc tcctcgtagc tgttgcaagc attaagtgcg gcaaccgcct
    29581 gacgcaccat cgggttgagc tgagaattga aatcgggctc aaccagcgcg tcatccaacg
    29641 cgccgtccaa tatttcctga tgcctgcccg tagccttaga tggtacggtg tgggcggata
    29701 aagccgccaa tgcagttctg tttatcggat tgtccggtac ctgccgcacc aacacagcct
    29761 caccctcctg cacatctgga atgaccagtt tgtcgcgcac ccagctttcg gggatttgta
    29821 cgccgacatc caccagtttc gggatagcgt cggcaaagac cgcgatgtct ttcggctcgc
    29881 gcgtgtcaaa ttcaaatttc ggcacgcggt ttgggtcggc attgggatag ttgatttgca
    29941 ggaacggtcc gatgatttgg cttgtgatgg tttgcgccac ctgttttgcg tccgacacca
    30001 gcaaatcgcg gcgtacctcg ttgtggatat tgcccagcgc gttggtgctg gattttccgt
    30061 ccgcaccgct ggttagcgtt tgccccaaaa tcagccgcgc cgccgatttt tcgcaccagt
    30121 cggccatctg caaaaacgga ttgctggttg ccatcgtacc gcttgcagcg ttgtgcagct
    30181 cgatttccat accttctggc atgatgcctg ccgcgttgtg accgatttcc gccaccgctc
    30241 gaagcagggt gtttttttcc tctttggttg cgcccgcgcc gtatttgccg atacggatgg
    30301 gcatgccgta cagctccaaa aactcggcaa aatcgtggac ggcgtagtgt ttgaacatat
    30361 acagccagga aagcgtgcgg aaaagcccgt tgcgcgcctg ctggacgctg cgcgattttt
    30421 gggtatgaac gacccagccc agcggccaca acgcttcgcc ttccggattt tcgcgggtac
    30481 gcagcagcag cccgttgtct ttgtcccatt tgaaccagct ttgcgggcgg tggataaagt
    30541 ttcggggtag gtaaaggccg tctgaaaata cccactcgac ctccaacgca gaaaatccgt
    30601 gccctaccgc gtccatcaaa tccataatca ggtcttcgag ggtaggcagg ctgtccatca
    30661 tttcgtaggc ttggtcggac agcttttctt cttcgggcgt cgcatttcgc ggcggggcga
    30721 cgcgccagtt gagcgtcagc agcgcgcgtt tgcgcgtccc catatttgcc gcgatgtcgc
    30781 tgtcgcgctc ctcaatgtcc gcgaaaagct cgtgttgggc gcggatgtcg ccgctttccg
    30841 catcctcgaa gagggcccgc atcttttgcg gtgtaataaa attggacgga tgctcggcga
    30901 taacccgacc ggtcgccgta atttgagcca cgtccgtctg caatgcagct tcgggctttt
    30961 ggattttagt tttattgttc ttttttgcca ttatcggctc catttacttt ttatctcgtt
    31021 accaaaatcg ttatcggtag ggctttgcca ttctatcggc acacaattgg ccaccgcccc
    31081 cgaccagagc atatgcactg catcaggacc atcatcatga tcagcctttg gaaaatggcg
    31141 gaactgctgt atcagcgtct gttggctctc attcaacaga atcaaaccgt tcgccatgtg
    31201 aggctgtaaa gtctcaatcc gcaagagctt gtccgatacc ggcttgaccg cccgcgccgg
    31261 gacaggtatt ccacgcgccg cgctgcgctt gaccagctca tctttcaaaa attcctgaaa
    31321 ctgaaccgtc tcaacaaacc acagtttgca acgatattga cggtgcaatc tgataacgtc
    31381 ctcaataatc aaatcaggga gacgtttttt aatctgagct tccacgacat acagtttgcc
    31441 ggtttcacgt tgataaccgc caatgataat cgccgacggg tcacggctcg cccccgcttt
    31501 acccagcgac gggtcgagcg caccgaaata caccaaatcg gacggcaggt cgttccaaaa
    31561 cttcatcgac ttggcaaacg gcgcatcttc gccgctgacc gggtcgtttt gatattcgct
    31621 gtcaaatgtc gcatgaccgt cacgcgcacg gattttcatc agcgcgagta cgccgcgagc
    31681 cgcccagctt gtttgcgcac cgcgctccat ctcgtcttta ttggcgagat aaaacgcctc
    31741 ggccacagtc tcgccgtcgt ttcggaaaag ttcctcccat ctgtcccaca aatccatgcg
    31801 gtcgggccag cgtttcatcg ccttaaactt aataccgtgc caaaacgggt tattcaaagt
    31861 gcggttaagt acgctgtcgt aatgcaaaat cgtgccgata tatatcacat cgtatttctg
    31921 gccgacaccg cccaaaggca atacagtctt agtcagccac gcattgagct tgtcgcgctg
    31981 ttcggggttg cggacttgct cgtcattctc aatatcgtcc aatatggtca ggtcaggacg
    32041 gtaagggccg tgacgcaaac cgcgcagctt tttaccgcta ccggccactt ggactttaac
    32101 gtcattggcc gtcacaatcg taccggcctg ccatacgcgg ccctgcccgc atacttccgg
    32161 aaagtcggtt ttcaagcgcg gattaaattc caattccgcc ttgatggctt cgagcatcgg
    32221 gtatgcctga tctatgctgt ccatcacaat aacggcataa tgtttttggc ctgtcacaat
    32281 acaccacagc gtaaacaact gagtaacctg cgtcgactta ccctcgccac gcggcgcacc
    32341 caccgcctca ttttcccctt tgggggagcg gataatctcc ggcagacggc tgaataaaaa
    32401 cgcatgcagt tcggattttt caggggagcg gatataatgc ggaaagtagg tgttgacgaa
    32461 atactcgtaa ccgcctaccg ggtcaaacac cttggcacgg cgtgcagcaa tagccttggg
    32521 cgacgcatcg aagccgtcca cttccgcttc gatgacttgg cgcaggctgg cggccagttc
    32581 ggcaagggat ttgaggaatt cttttgtttt catgcattta aaaagtctta atcaattttt
    32641 cggcaatcca gcgtgagacc ggcggacaga cggcgtttcc cgcagcttga gcctccgcag
    32701 tgttggcctc atccattccg aggagaagcc catcatggtt agacgctctt tgccgctcaa
    32761 ccatcgcacc ccgtccgttg ggcgtaacga gaatatttgc gcctgagcgg tcaatggctc
    32821 cattggaaat ccctctaagc aaagtaccat gcggcttttg cagcccgcac gtgtcaaccg
    32881 agcgaggtaa gactccaatt gagccggcgt cagccataaa ctcatagggg gggggtactc
    32941 tcccaatcca gcgaccaaga aaactcgacg gcgtgccgtg gggactccga aatatgctgc
    33001 attaagcacg cgccagtatc ccacataccc gcattcggca agggacttga tgactgtttg
    33061 aaagtcttcg ccattgttgc tattgagcaa acccgtaacg ttttcaagga caatccagcg
    33121 gggtttaagc tggtcgacaa tacgcattgc gtcataaaat aaacctgttc tttccccggc
    33181 aagaccttta cgcttaccgg ctattgatac atcctgacat gggaatccgc caataatcac
    33241 atctaccggc cataattctg acaataccgt tctgacatcc tcaaactgtt ttgcgtatgg
    33301 gaagcggtcg gtcaaaactg cacggggtac aggattaatc tctacttgcc aagcagtgtc
    33361 aaaaccagcc tgttcaaatc ccaaatcaaa cccgccaata cctgcaaaaa ggcttccgac
    33421 ggtaggtttt tgcttatcca aacttcttct ccacctccac cccaaacggc tccaatacct
    33481 ccacaaaagc aggcaaatgt ttgggatgtt tttcttgcac aaacgccatc aagaactcaa
    33541 tcaattccaa agccgtcgcc agttttgacg tttccggcat cacacgcgca ttggcggata
    33601 cggttttcgt aaacgcatcg gccaggctgg ccaacagctt ggcgcggtcc gatggcggca
    33661 aatcttcggt actcgaatcc tgcagcatcg tcatcgtgct gttgtactgc accataaaac
    33721 cggccaacat cgcacggctc aagtcttcaa taccgccgtc ggccaaagtg taggaggcgc
    33781 gcatcttatc ccagtcgtcg cctttttcct tatccgcacg tttccacgca cgcgcagtgg
    33841 tctgcgggat ttcgcacatc aaggccgccg tttccaaagt ttgctcgccg ctcacataga
    33901 gccggcgtaa cttttcacgg atttcttgcg ggtgagccat aattacagcc ccattttcgc
    33961 tttaagcaat tcccagccga ccgtaatcac gccgccgccc agtgcgccga atgtaatggc
    34021 cgtgcgtttc gtgtcttggc ggatttgtgc aatttccgcc tgcatttcct tctgattttt
    34081 cagcgtttga tcagtcttgt tttcaatacg cgccaaggct tccaaaatcg gggcactcat
    34141 gatttgtctg ctttcctgtc cagtttttcg tttacttttt ccaacttgtt ttcgatgcgt
    34201 cccaacgaag ccgcaatatt gtcgcggtct gccttggcat cttgcttggt gtgataagag
    34261 agcttgaccg cgtgcagctc ctctttcagg tcgtcgatac gcttgtccgc ctctttcaga
    34321 cggcctgaaa tgccgttgac ccaaaaccaa aatgccgccg tcgcaatcgg ccacagggtt
    34381 ctgaaaccaa attcaaagtc catttaaaac ccctttaaac cggcacatcg ccgaatacga
    34441 tacggacggc gtaaccgtca gggcgattgc tggcaatctc cagtccatca ccatcattac
    34501 aaacacagta atacgccccc aaagtttgcc aaaccgcacg tttaaaggtg ttatagttcg
    34561 taattggata ttcaaggtga aacgtcgtct gaaaatcctt atccatgcgt accgcgtact
    34621 caatgcctgc cttatccaac agatcggaaa catgaatgac aaacggctct tgttcgcgtg
    34681 cgcggcttaa gcccaattcc aaatcggcat ggcgcacagc caactgacgt tcaactaatt
    34741 cacggtaggt cattctttga tacccattaa atattttgtc cgcttgtaca atttcttaac
    34801 ccacgaaata tttacaaatg tataaacctt tgctacaact tcgccgtcat agtgcgcatt
    34861 ttctcgtgca gcccgaaatt tcgccctggc ttcttcaggg ttgtccgccc aaatgctcaa
    34921 tgaccaggac ttgccgttaa agcggtaaga aaacgtgtac tcattcatag gagaaacctt
    34981 atgtattttg aaatctataa agacgcaaaa ggcgaatacc gttggcgttt gaaagcagcc
    35041 aaccatgaaa tcatcgctca gggcgaaggc tacaccagca agcaaaactg ccagcacgca
    35101 gtcgatttgc tgaaaagcac taccgccgct acccctgtaa aagaggtata aaatccgctt
    35161 ttaccctcag cccgcgccct acgcgggctt ttttgtcagt ttccgacttt gcgggagtga
    35221 ttgcccgccc agtcgcgcca agcctgattt tggttttcaa gttcggcaac atagccgcca
    35281 aactcagcgg cgtgttccaa cagcgttact gtcttgccgt ctttcggcgg attcgggcgt
    35341 accggcgcaa ccatcaatgc ggcaggcggt gtcggcaaga ccgccttttc gacaacctta
    35401 atttccgtag ccgagggcgc gcttgtagag ctgcaagccg tgagagccaa agccgtcaat
    35461 acaaccgccg cctgcatttt tacggtcttg agtaaggaca ttttcgattt cctttttatt
    35521 ttccgttttc agacggctga cttccgcctg ttttttcgcc aaagccatgc cgacggcgtg
    35581 cgccttgact tcatattttt tagcttccgc acgcgcctgt tccagctcgc gtgcatagtt
    35641 ttgagccgac aacagcaggg cttgcgcttt gtctttttcc atcttgtcaa tgaccgcctg
    35701 ctgcttcgca aaagccgact tgtagcc
//
LOCUS       DAWFBO010000003        35159 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFBO010000003 DAWFBO010000000
VERSION     DAWFBO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35159
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.003"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(144..234)
                     /locus_tag="WGO87_000101"
     tRNA            complement(144..234)
                     /locus_tag="WGO87_000101"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:gga)
     gene            complement(273..359)
                     /locus_tag="WGO87_000102"
     tRNA            complement(273..359)
                     /locus_tag="WGO87_000102"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:cag)
     gene            complement(395..481)
                     /locus_tag="WGO87_000103"
     tRNA            complement(395..481)
                     /locus_tag="WGO87_000103"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Leu,seq:cag)
     gene            570..2945
                     /gene="rnr"
                     /locus_tag="WGO87_000104"
     CDS             570..2945
                     /gene="rnr"
                     /locus_tag="WGO87_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0391538.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRQ
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="WGO87_000105"
     CDS             complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="WGO87_000105"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0391539.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     ALIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRNRLV
                     TVPEGTSIDEAREIMHAHKVERVLVLNDQDELKGLITVKDILKTTEFPNANKDAEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVSEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSADKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4894..7452)
                     /gene="glnD"
                     /locus_tag="WGO87_000106"
     CDS             complement(4894..7452)
                     /gene="glnD"
                     /locus_tag="WGO87_000106"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0391540.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVGELCESVRDDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGISDARQFAADHF
                     LTEEESDLLAWLVENHLLMSIVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            complement(7501..7821)
                     /locus_tag="WGO87_000107"
     CDS             complement(7501..7821)
                     /locus_tag="WGO87_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0391541.1"
                     /translation="MMGNKLTPPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8146..8619)
                     /gene="bfr"
                     /locus_tag="WGO87_000108"
     CDS             complement(8146..8619)
                     /gene="bfr"
                     /locus_tag="WGO87_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0391542.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8647..9111)
                     /gene="bfr"
                     /locus_tag="WGO87_000109"
     CDS             complement(8647..9111)
                     /gene="bfr"
                     /locus_tag="WGO87_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0391543.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9575..10558)
                     /gene="lipA"
                     /locus_tag="WGO87_000110"
     CDS             complement(9575..10558)
                     /gene="lipA"
                     /locus_tag="WGO87_000110"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0391544.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10551..11174)
                     /gene="lipB"
                     /locus_tag="WGO87_000111"
     CDS             complement(10551..11174)
                     /gene="lipB"
                     /locus_tag="WGO87_000111"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0391545.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11176..11451)
                     /locus_tag="WGO87_000112"
     CDS             complement(11176..11451)
                     /locus_tag="WGO87_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0391546.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11636..12706
                     /locus_tag="WGO87_000113"
     CDS             11636..12706
                     /locus_tag="WGO87_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0391547.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(12813..13760)
                     /locus_tag="WGO87_000114"
     CDS             complement(12813..13760)
                     /locus_tag="WGO87_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0391548.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(13775..14182)
                     /locus_tag="WGO87_000115"
     CDS             complement(13775..14182)
                     /locus_tag="WGO87_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0391549.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            14263..14922
                     /gene="ung"
                     /locus_tag="WGO87_000116"
     CDS             14263..14922
                     /gene="ung"
                     /locus_tag="WGO87_000116"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0391550.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            complement(15430..15945)
                     /locus_tag="WGO87_000117"
     CDS             complement(15430..15945)
                     /locus_tag="WGO87_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391551.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(16411..18333)
                     /locus_tag="WGO87_000118"
     CDS             complement(16411..18333)
                     /locus_tag="WGO87_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0391552.1"
                     /translation="MIEIKNLTLQRGLKILLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALLKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(18410..18805)
                     /locus_tag="WGO87_000119"
     CDS             complement(18410..18805)
                     /locus_tag="WGO87_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ0391553.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            18978..20285
                     /locus_tag="WGO87_000120"
     CDS             18978..20285
                     /locus_tag="WGO87_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0391554.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREVRISAVCD
                     LSEEKARQICPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGHDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            20278..20712
                     /locus_tag="WGO87_000121"
     CDS             20278..20712
                     /locus_tag="WGO87_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391555.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(20771..21040)
                     /locus_tag="WGO87_000122"
     CDS             complement(20771..21040)
                     /locus_tag="WGO87_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0391556.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(21222..23672)
                     /gene="lon"
                     /locus_tag="WGO87_000123"
     CDS             complement(21222..23672)
                     /gene="lon"
                     /locus_tag="WGO87_000123"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0391557.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALLGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            23923..25026
                     /locus_tag="WGO87_000124"
     CDS             23923..25026
                     /locus_tag="WGO87_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0391558.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQADLRVADVL
                     KTKPVHKKAADPDDPLAFARNRETTFLYRLKKA"
     gene            complement(25098..26843)
                     /gene="recD"
                     /locus_tag="WGO87_000125"
     CDS             complement(25098..26843)
                     /gene="recD"
                     /locus_tag="WGO87_000125"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0391559.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSGSEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQPFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(26911..27606)
                     /gene="lolD"
                     /locus_tag="WGO87_000126"
     CDS             complement(26911..27606)
                     /gene="lolD"
                     /locus_tag="WGO87_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0391560.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRLLLMGEDLRTLSQRQLGILRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(27599..28846)
                     /locus_tag="WGO87_000127"
     CDS             complement(27599..28846)
                     /locus_tag="WGO87_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0391561.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            29051..29329
                     /locus_tag="WGO87_000128"
     CDS             29051..29329
                     /locus_tag="WGO87_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391562.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(29385..30002)
                     /gene="recR"
                     /locus_tag="WGO87_000129"
     CDS             complement(29385..30002)
                     /gene="recR"
                     /locus_tag="WGO87_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0391563.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(30069..31607)
                     /locus_tag="WGO87_000130"
     CDS             complement(30069..31607)
                     /locus_tag="WGO87_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0391564.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAMQNEQADGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(31662..32036)
                     /locus_tag="WGO87_000131"
     CDS             complement(31662..32036)
                     /locus_tag="WGO87_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0391565.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAGTQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGRV
                     YVGFSEETYDGIFKRQAPCRQG"
     gene            32126..33814
                     /locus_tag="WGO87_000132"
     CDS             32126..33814
                     /locus_tag="WGO87_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0391566.1"
                     /translation="MLQSENSKNLISCSIKALPIMISTNGITMQFGAKPLFENVSVKF
                     GEGNRYGLIGANGSGKSTFMKILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVL
                     DVVMMGHTEMWAAMTERDAIYANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLS
                     GVGISEDLHNAKMAEVAPGFKLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVL
                     NQYDSTMIIISHDRHFLNEVCTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNA
                     KAKEKLQELQEFVARFSANKSKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEAD
                     EKAKLHRQAVEVEKLAKLFETQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAY
                     NPEYSDGLLPDEGTIKWAEKASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIR
                     GTLGRLLFGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESL
                     NMALEKYNGTLIFVSHDRQFVSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            33886..35139
                     /locus_tag="WGO87_000133"
     CDS             33886..35139
                     /locus_tag="WGO87_000133"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0391567.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFSA
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEVANQVDSGKIAAECRAQGKVHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         7759 a         9081 c         9587 g         8732 t
ORIGIN      
        1 taccgcaaaa cataccataa aaaaaacggt aacggatagt tgtaagcggt tgatgacgat
       61 tatagacaat aacgggtttt cccaatgaaa ttattgtttg aataataaaa aatcccaaac
      121 cgtaaaagtt tgggatttgt atttggcaga gaggaaggga ttcgaaccct cgatacgcta
      181 ttcacgtata cacgctttcc aggcgtgcga cttaaaccac tcatccacct ctctaatggc
      241 ggaaattatc ccaatcggga taatttatta tttggtgccc gggagaagac tcgaacttcc
      301 acacccatga ggataccagc acctgaagct ggcgcgtcta ccaattccgc cacccgggca
      361 atctaaatat tgaaataaag caaagcattt gatttggtgc ccgggagaag actcgaactt
      421 ccacacccgt gaggatacta gcacctgaag ctagcgcgtc taccaattcc gccacccggg
      481 ctttgcttta tttgctgttt tgcggtacag tgtcctgccg caaagaagtc gctattatat
      541 agttcataaa gagaatgtca acagtccaaa tgaataaaaa tattaaatct ttaaatttac
      601 gggaaaaaga cccgttttta agtcgtgaaa aacagcgtta tgaacatcct ttgcccagtc
      661 gggaatggat aatcgaattg ttggagcgca aaggtgtgcc ttcaaaaatc gaatcgcttg
      721 cgcgcgagct gtcgattacg gaagacgagt atgtcttttt cgaacgccgt ctgaaggcga
      781 tggcgcggga cggtcaggtt ttaatcaacc gtcggggcgc ggtttgcgcg gcggacaaat
      841 tggatttggt caaatgccgc gtcgaggcgc ataaggacgg tttcggtttt gccgtgccgc
      901 tcacgcccgc caaagacggt gattttgttt tgtatgaacg ccagatgcgc ggcattatgc
      961 acggcgatat tgtcactgtt cgtcctgccg gcatcgaccg taggggccgc cgcgaaggga
     1021 cagttctgga tattgtcgaa cgcgcgcaaa gcaaagtggt cggccgtttc tatatggata
     1081 ggggcgtggc gattttggag ccggaagaca agcgtctgaa ccaaagcatc gtattggaac
     1141 cggacggcgt ggcgcgtttc aaacctgaat ccggtcaggt catcgtcggc gaaattgagg
     1201 tttatcctga gcaaaaccgg caggcagtgg caaaaatcat cgaagttttg ggcgattatg
     1261 ccgacagcgg catggagatt gaaattgccg tgcgcaagca tcatttgccg caccaattca
     1321 gtgaagcgtg tgccaaagcc gtgaaaaaaa ttcccgacca tgtacgcaaa agcgatttga
     1381 aaggccgcgt cgatttgcgc gacctgcctt tggtaacgat tgacggcgaa acggcgcgcg
     1441 attttgacga tgcggtgttt gccgaaaaaa tcggacgcaa ttaccgtctg gtcgtggcga
     1501 ttgccgatgt cagccattat gtccgccccg atgacgctat cgacacggac gctcaggaac
     1561 gcagcaccag tgtttacttc ccgcgccgcg tgattcccat gttgccggaa aacctgtcca
     1621 acggcatctg ctcgctcaat cctaatgtcg agcgtttgtg tatggtgtgc gatatggtca
     1681 tcacttacgc gggcaatatt aaagaatacc gcttctaccc cgccgtgatg cgctctcatg
     1741 cccgcctgac ctacaaccaa gtttggaaat ggctttcagg cggcatcggg catccgttca
     1801 aaacacaaat cgacacgctt tacaaactct tcaaaatcct tcagaaaaag cgtttcgaac
     1861 gcggggcggt ggagtttgac agcatcgaaa cccaaatgct tttcgacgac aacggtaaaa
     1921 ttgaaaaaat cgtccccgtt gtccgcaacg atgcccacaa gctgattgaa gaatgtatgc
     1981 tggcggcgaa tgtttgcgcg gcggattttc tgttgaaaaa caagcatacg gctttgttcc
     2041 gcaaccattt gggccccacg cccgaaaaac tcgccaccct gcgcgagcag ctcggtctgt
     2101 tggggcttca acttggcggc ggcgacaacc cgtcgccgaa agactatgcc gcgcttgtcg
     2161 aacaattcaa aggcagacct gatgccgaat tgctgcaagt catgatgttg cgctccatgc
     2221 agcaggcggt ttacgaaccg cattgcgacg gacactttgg tcttgcctac gaagcatacg
     2281 cccacttcac ctcgcccatc cgccgctatc ccgacctgac cgtacaccgc gccatcaaag
     2341 ccgtgttgaa tcagcaaacc tacacgccaa aaaaaagctg gcaggctttg ggcgtgcata
     2401 cctcgttctg tgagcgccgt gccgacgacg ccagccgcga cgtggaaaac tggctgaaaa
     2461 cctattatat gcgcgataag gtcggcgaag tattcgaagg taaaatctcc ggcatgacca
     2521 gttttggtat ctttgtaaca ctggacggca tccacattga cggcttggtg catatcagcg
     2581 atttgggcga agactatttc aacttccgcc ccgaaatcat ggcaatcgaa ggcgaacgca
     2641 gcggcatccg tttcaacatg ggggacaggg ttgccgtccg ggtcgcccgt gccgatttgg
     2701 atgatggaaa aatcgatttt gtcctgattg ccgaggggag cggcaggggg cggaaagtta
     2761 aatcatccgc gtctgccaaa ccggcaggga cggcggggaa agggaaaccg aaaaccgccg
     2821 ccgagaaaaa aacagcccga ggcggcaaag taaggggaag gggcgcgtct gccgccgcag
     2881 aatcggggaa aaaggcaaag aaaccggttc cgattaaggt aaaaaaacgg aaaggcaaat
     2941 cataatgctg acggggtggc ttggggaggg cggggcatac tttcccgaag ccgccgcccg
     3001 attttttgat atggcggccg gcgcatcatt tcaggccaat cgaaacgccc gtattgaaag
     3061 attgcgttta tttccaccgc cgttttaaag gccggcggta ttcggcagac ggggcgcaaa
     3121 cggcgttcag acggcatttt cattctttcg gcgtgtccgt ccgaattgct ttgcccgtcc
     3181 gcgcaatcag cccgtgcggc ttgccttgaa cggacaaaaa atgccgtctg aaacccgaaa
     3241 atcaggtttc agacggcatt ttgccttgaa aaggctgttc aaatcagcgg tggtagttcg
     3301 gtgcttcttt agtgatttga acgtcgtgaa cgtgcgattc gctcatacct gcggaagtga
     3361 tttccacaaa ttcggctttt tcgtgcattt cggcaatatt ggcgcaaccc aagtaaccca
     3421 tactggaacg cagaccgccg gtcagctggt ggatgatgtt cacaatcggg cctttgtaag
     3481 gaacgcggcc ttcgatgcct tcggggacgt atttgtcggc gctgtcggtt ttgtcttgga
     3541 agtagcggtc ggcagaacct tggctcatcg cgcccaatga acccataccg cgataggatt
     3601 tgtaagagcg gccttggtag agttcgattt cgcccggtgc ttcttccgta ccggcaaaca
     3661 taccgccgag catgacgctg tatgcacctg ctgccagagc tttggcaata tcgccggaga
     3721 agcggatacc gccgtcggca atcaggggaa cgcctgtacc tttcagggct tcggaaacat
     3781 tgtgaatcgc agtcagttga ggtacgccca cgccggccac gatacgggtc gtacaaatcg
     3841 agcccgggcc gataccgact ttaacggcat ccgcacctgc agcgaccaaa tccaaagcgg
     3901 ctttggcagt cgcgatgttg ccgccgatga cttggatgtg cggataagtt tctttaaccc
     3961 agcgcacgcg atcgataacg ccttggctat gaccgtgggc agtatcgacc acaatcacgt
     4021 ctacgccggc ttctaccaat gctttgacgc gctcttcggt atcgccgcca gtacccaccg
     4081 cggcaccgac gcgcagacgg ccttcggcat ctttgttggc attagggaat tcggttgttt
     4141 tcaaaatatc tttaactgta atcagacctt tgagttcgtc ttggtcgttc aacaccaaaa
     4201 cgcgctcaac tttgtgcgca tgcatgattt cgcgggcttc gtcgatgctg gtaccttcag
     4261 gtacggtcac caaacggttg cggggggtca tgatggcaga aaccggcaga tcgacacggt
     4321 tttcaaaacg caggtcgcgg ttggtcacga taccgaccac tttgccgttt tcaacaacag
     4381 gcagaccgga cattttgcgt ttgcgttgtg cgcgcatttc caagacttcg cggatcaatg
     4441 cggtcggcgc aacggttacg gggtctttga ccacgccgct ttcgtggcgt tttactttgg
     4501 aaacggcgcg cgcctgcatt tcgggcggca tgtttttatg gatgatgccg atgccgcctt
     4561 cctgtgccat cgaaatggcg aggcgcgcct cggtaacagt gtccatcgcg gcggaaagca
     4621 gggggaggtt gagtgtgatt tcgcgggtga gcttggtttg aagtttaacg tctcgcggca
     4681 gcacggtcga atgtgcggga accaacaaaa catcgtcgaa agtataggct ttttctacga
     4741 tacgcataat gctcggtctt tcagtttgtg caagatgcac ggcattatag cacgttaccg
     4801 gcggcttgac agtttatatt gtttgcaata tcccccgcct tcagacagcg tacggcgcgg
     4861 cggcgggata ccgtctgaaa accggcaggg tgcttatgtc aactgttcca gcaaagcctg
     4921 cttcaatgag gactggattt ttgggttttt caggtcgggg ctaaaaacgg taaaactgtc
     4981 ttcggcgcgt tcatccagtg tggagatttt ggcatagcgc aggctgacgt tgtgggcgaa
     5041 aaagacttcc gccatatcgg cgagcaggaa ggggcggttg acggcggtaa tttcgacgga
     5101 gtaccagtcc ggatagtctt cttcgggggt gatggtgatg cttggcgcga tcggcatgta
     5161 gcggctgcgg cggctgatgc ggcggctatg gctggagatt tcggcaacgg tgtgtccgtg
     5221 gataaagctg ttgagttcgg cttcgagcgc gctttggatg tcggggtagt cttcgggagc
     5281 gtgctgcgag gggatttgca cgatgaaggt gtcgaggatg tagtcgtgtt cggtaataaa
     5341 ggcgcgggcg gcgaggatgt cgaagccgtg gcggctgaag atgcggcaga ggcgggcgaa
     5401 caggcgcggg ccgttgggca tgaaaaccat gacttgaaag ctgtcgcttt tgggcaggat
     5461 gcggctgcgg acgatggggg tttcaaagtc gtggactagg ttggcggcgt gccacaggat
     5521 ttcgcgggac tggtggcggg cgaagtaggc ggaaccgagc gcgttccata gttttttctg
     5581 ctgtttttcg gggacggcgg cgcgggtgag taagtcggcg gcttcctgcc ggcggcggcc
     5641 gaagagggtg tgcgggttgc cgccgttgcc tgtaaggtag cgtccggcgg catggaagag
     5701 gctttccagc aggctggcgc gccatgcgtt ccacagcttg ggattggtac cgcgtatgtc
     5761 ggaaatggtc agaaggtaga gcgcgctgag gcgttcgtgg gtttggacgc gtttgcagaa
     5821 ggcatcgagt acgtcggggt cttggatgtc ttctttttgg gcgacgatgg acataagaag
     5881 atggttttcg accagccagg caagcaggtc gctttcttct tcggtcagga agtggtcggc
     5941 ggcaaattgg cgcgcgtctg agatgccttg tatggcatgg tcgccgccgc gtcctttggc
     6001 gatgtcatgg aaaaaggcgg caaggtagag gatgtcttgt ttttcaaagg actgcatcag
     6061 tgcagaggcg tagggcagct cgtggctgtg catatccaag gcaaggcggc ggacgttgcg
     6121 gacgacggcg aggatgtggt cgtccacggg atagatgtgg aacaggtcgt gctggagcag
     6181 gccgacgatt ttttcccacg cgggcaggta gcgtcccaat acgccgtaga ggttgaggaa
     6241 gcgcagggtt tgggtcagtc cgttgccgtt gcggaaaaag ccggcgaagc ggtggcggtt
     6301 ttcagaattt tggtagaagc tgcggttgat tttgcgcgtc gcgccccacc atgcgcgcag
     6361 ggtttgcggt tcgagcgcgg taatgtcgtt gcgctgctgc atgatttcga cgattttgaa
     6421 aatgtgttcg ggccgtctga aaaaaatatc ggtgtgccgc gcggcgattt ggttgttgac
     6481 ttggatgtag tcgtcgtcaa tccgcagggt aacgcgcaac ggggtggagg aaacgcggct
     6541 ttgcagcata ggcgtgagga tgccgcccag ttgtttgacg gttttaatcg cgcggtaaaa
     6601 cacgcgcatc agttcttcgc tttggcggcg gaggttcaag ccttcataac ccatgctttc
     6661 ggcgacttgc ggctgcaaat cgaacagcag gcggtcttcg gcgcgcttgg cgtttaaatg
     6721 cagatggatg cggatgtggg cgaggcggcg gtagccgtgc gaaagcatac cggcttcggc
     6781 acgcgtcaaa atccgctgtt tgagcaggtc gggcaggtcg gtcgccaagc cttgcgcctt
     6841 cgctatccaa agcagggtgt ggatatcgcg cagaccgccc ggacagcttt tgatattcgg
     6901 ctccaatact gcccccgaac cttgcgattt ggcgtggcgg tgttccatct ccaccagttt
     6961 tgcctcgaca aacgccgcca cattgcgctg cgcgttcatt ttttccgcta gtttatccgc
     7021 cgtttggcgg ttgccaaaca aaaacctagc ctctaaaaac gccgtgtccc ccgtaatatc
     7081 gtcgcgcacg ctttcgcaca gttcgccaac gctgccgctt tttacagacg gcatcagttt
     7141 gcagtcccac agggtttgga caaaccgggc aatctgttcc tgaatgccgt ctgaaagcgg
     7201 ggcaggggag acaaccgcca aatccacatc cgaacaggga tacagttcgc cgcgtccgaa
     7261 gccgcctacc gccatcaggc ataacgcgct gttttgaaaa tactctgccc acaatgccgc
     7321 cagtaaggtt tcgactgccg ccgtgtattc tctgaaaaat accgacacgc ggttggcttt
     7381 caaataatgc gcttcggcgg catcgcgctg ctgtttgaag gtttccagtg ctgaagacag
     7441 gtttgcaggc atttttattc tttcgattgg cgggaaaaag ggaggcggat ggttcggcgg
     7501 tcaaataccg ctttcagacg gcatttgtcg ggtatcggcg gaattggtca tcaggttcaa
     7561 ccgttcctgc ggcggcagca gcaactgata cgccgccacg cccaaagccg ccgccatttt
     7621 ttcgatattc gacagggcga tgttccagcg tttgcgttcg actgccgaca cataagtcct
     7681 gtccaaaccg cattgccgcg ccaattcttc ttgcgaccaa cccttgttca cgcggaaaag
     7741 ccgcatattg tatgccaata ccgcccgcaa atcctgttcg tcaggcagtt cggcaggcgg
     7801 ggtcaatttg ttgcccatca tttgcttcca gataaatggt taaaattaag catttgctgt
     7861 tacggatttt acttcacata aaagccaaaa aaaaggggtg gcggcagcca cacccaaaca
     7921 cacacacatc aagaggaaag aggtaaaatc aagacagatt gggaaacagc aaacaagcaa
     7981 tctagatttc atatcggtat tatcaaacaa aaaccgttat cgagtattga ttcaaatcaa
     8041 tttagatttt attcgcaaac cggcaaagaa aaacggcata cccgtttata cggatatgcc
     8101 gtctgatgat gcaggggact gctgtatttg gcagtggttt gtgttttagt cctcttgcgc
     8161 cgctgtttgc aggtagttcg gcaaaccaat tttgccgatt agttcctgct gggtttcgag
     8221 ccagtcgatg tgttcttcgt tggtgtcttt ttgtttttcc aacaaatcac ggctgacgta
     8281 atcctgttgc gcttctgctg tggcgatggc ggcaagcagg gcttcgtgtt tttcctgttc
     8341 tttggtcaaa tcgcaggcga tgatttcttc ggtggactcg ccaatcagaa gcttgcccag
     8401 ttcttgcagg ttcggcaggc cttcaaggaa cagaatgcgc tcgatcaaat cgtcggcagc
     8461 tttcatttca acgatggact gtttgaagaa atgttcgccc agttcttcaa agccccagtt
     8521 tttcaaaata cgggcgtgaa ggaaatattg gttgatggtt accagcagca agcctaagtt
     8581 tttgtttagc tcgcggataa ccagacggtc gcctttcata cggactcctt ttattccgaa
     8641 tacggattac agttggcttt ggtagtagtt gccttcgcca attaactcga tcaggcgaag
     8701 ctgctgttcc agccagtggg cgtggtcttc ttcggtatct ttcagttggg caaccatcag
     8761 gtcgcgcgta acatagtctt gagcctcttc gcacagtttg atgccttttt tcaacgcatc
     8821 acgtacttca tattcggttt gcaggtcggc tttgaggcag gaaaccacgt ccgtgccgat
     8881 attcagttcg gcgcgtgcca ttttcggcgt gccgcccagc atcaggatgc ggcggatgaa
     8941 gtcttcggcg tgtgtggttt cttcttccat ctcgtggttg agacgttcaa aaagtttggt
     9001 gtagccccat tcggagtaga ggcgggagtg gataaagtat tggtcgcgtg ccgccagctc
     9061 gccagacagc aattcgttca tataatcaac aacagcttga ttgccttgca taatatctct
     9121 ctttctgtaa cttggttttc ggtatgtctg aacagcatct gtgtcgctat tcgggatgcg
     9181 tgcattgtat gcaaaagctg tcggcatgac aaattttcta tttaaaatac aaacaattat
     9241 caaaagaaat agggcgtatt ccccgatgcc ttccaaatca gtatgctgtt tcttatcggt
     9301 tttttgtgct gttaaaacta agaatccata tcatccataa agaacattta cacttgttaa
     9361 ctatagcgaa aaacaaatag agcacggttt tttatcaaaa tttataatga atcgttctca
     9421 ttaaccgaca gattcttttg agattatcgg attttgggaa taaattatgc cgtttgaggg
     9481 gctttcagac ggcatagaca attaaagctg attaaacaac gcggcagaca gcagcgtagg
     9541 aaattatttt ttacttcgga acggtatttt gggtttaatg atgcccgcaa ccgccgtggc
     9601 tttcgcgtaa ggcttcggca gcctgttcgt cggcgtggta gctggaacgt accatcgcgc
     9661 cgatggcggc attgctgaag cccagttcgt atgcttcttt ttcaaagatt ttgaactgct
     9721 cgggcgtaac gtagcgcagg acgggcaggt gtccgtctga aggctggagg tactgtccga
     9781 tggtaatcat ttcgatattg tgcgcccgca tatcgcgcat aatttcacgc acgtcttcgt
     9841 ctgtttcgcc caagccgacc atgatgccgg atttggtcgg gatgtgcggc atcatttctt
     9901 tataacgttt taataagtct aaagaatgtt gataattggc accgggacgg gcttttctgt
     9961 acaggctcgg atgggtttct aggttgtggt tcatcacgtc gggcggggtt tcggcaagga
    10021 ttttgagtgc gatgtccaag cgtcctcgga agtcggggac gaggatttcg attttggtgt
    10081 tcgggctggt ttcgcggatg gctttgatgc agtcggcgaa atgctgtgcg ccgccgtcgc
    10141 gcaggtcgtc gcggtcgacg gaggtgatga cgacgtaacg caggttcatg gctttgacgg
    10201 attcggcgag gtttttcggt tcgtcggggt cgagcatatt gggccgaccg tgtcccacgt
    10261 cgcagaacgg gcagcggcgg gtgcagatgt cacccataat catgaaggtc gccgtgcctt
    10321 tgctgaagca ttcgccgatg ttggggcagg aggcttcctc gcaaacggtg tgcatctttt
    10381 gttcgcgcaa aatgtctttg atttcaaaga atttgcgcga tgggagtttg gcgcgtatcc
    10441 attcgggctt tttcagtttc tcctgaaggg ggacgacttt gatggggatg cgggcggttt
    10501 tgtccgcgcc tctgagtttg atgccgcgtt tggggtcgtc ggttttgatt tcactcattg
    10561 ttgtctgctt tcggtgtgaa ttgtgtttca aggtgtgcgg taagtttggc ggcgacttcg
    10621 tccggcgtgg ggcagggttg gacaaaatcc gcgatttgcg tcatttccat accggcgtag
    10681 ccgcaggggt tgatgtgggt aaacgggctt aaatccatat tgacgttgag cgcaagcccg
    10741 tgatagacgg agccgttttt gatacgcagc cccagtgagg cgattttgcg ttctccgaca
    10801 taaacgccgg ggcgtttggg gtctgccgcc gcttcgatgc cgtattctgc caatgtggcg
    10861 atgatgctgt tttcaagcgc ggaaacgatg ttgcggacgc tggttttgcg ccgtttgaaa
    10921 tcaatcatcg tataaacgac caattgcccg ggcccgtgat aggtaatctg cccgccccgg
    10981 tcgatttgga cgacgggaat gtcgtcgcga atcagcaggt gttcgggttt tcccgccagt
    11041 ccttgtgtga acacgggcgg gtgttcgacg acccacagtt cgtcttcggt gtcagcattc
    11101 cgtccggcat taaaggtttt catcgcttca aaagtcggca gatattcgac caaacctttg
    11161 tgtatgattt tcatctcaaa gtaccacttt gaccagttcg tgcgaagtca gcgcgcggta
    11221 gatgttgtcc aattgttctt ggttttcaac ctttacctga acggtggcgc cggtatagtt
    11281 gcctttgctg ctcggacgcg tggtgatgtg gtgcgcctgc gtgtcggggg cgtggaggcg
    11341 gacggtgtct aaaaccgcct gctcgaactc gggatgcacc gcgcccatta ctttcaatgg
    11401 gaaggtgcag ggaaattcga tgagggatgt tttgtttttt tgttcggtca tgatgtgctg
    11461 ccttgtcgtg tacggtatgc cgtctgaagg cgggtttgcc tttcagacgg catcggatgt
    11521 gcgttatttt agcctaaacc gcgataacag gctatcggga aggcggaggc ttttttgacg
    11581 gcgcggcggt tctgctatac tggcgcacaa tattattttt agaagggtgg tttttatgta
    11641 tcggaggaaa gggcggggca tcaagccgtg gatgggtgcc ggtgcggcgt ttgccgcctt
    11701 ggtctggctg gttttcgcgc tcggcgatac tttgactccg tttgcggttg cggcggtgct
    11761 ggcgtatgta ttggaccctt tggtcgaatg gttgcagaaa aagggtttga accgtgcatc
    11821 cgcttcgatg tctgtgatgg tgttttcctt gattttgttg ttggcattat tgttgattat
    11881 cgtccctatg ctggtcgggc agttcaacaa tttggcatcg cgcctgcccc aattaatcgg
    11941 ttttatgcag aacacgctgc tgccgtggtt gaaaaataca atcggcggat atgtggaaat
    12001 cgatcaggca tctattattg cgtggcttca ggcgcatacg ggagagttga gcaacgcgct
    12061 taaggcgtgg tttcccgttt tgatgaggca gggcggcaat attgtcagca gtatcggcaa
    12121 cctgctgctg cttcccttgc tgctttacta tttcctgctg gattggcagc ggtggtcgtg
    12181 cggcattgcc aaactggttc cgaggcgttt tgccggtgct tatacgcgca ttacaggcaa
    12241 tttgaacgag gtattgggcg aatttttgcg cgggcagctt ctggtgatgc tgattatggg
    12301 tttggtttac ggcttggggt tggtgctggt cgggctggat tcggggtttg ccatcggtat
    12361 gcttgccggc attttggttt ttgttcccta tttgggcgcg tttacaggac tgctgctggc
    12421 aaccgtcgcc gccttgctcc agttcggttc gtggaacggc atcctatcgg tttgggcggt
    12481 ttttgccgta ggacagtttc tcgaaagttt tttcattacg ccgaaaatcg tgggagaccg
    12541 tatcgggctg tcgccgtttt gggttatctt ttcgctgatg gcgttcgggc agctgatggg
    12601 ctttgtcgga atgttggccg gattgccttt ggccgccgta accttggtct tgcttcgcga
    12661 gggcgtgcag aaatattttg ccggcagttt ttaccggggc aggtaggcgg ttccgaaaca
    12721 tatttgaagc ggaatacaac cttgcccggg tttaaataaa aatgccgtct gaaccccgaa
    12781 aacagagctt cagacggcat tttcatcacg gcttatttgg cggttttgct gctgtcgata
    12841 attttcatac cggcagaaat caggctgccg atgtcggcaa cattggcggg cataatcagc
    12901 gtattgcttt ctttggcaag attgttgaac gcggcgacgt attgttccgc aatcttcaga
    12961 ttgaccgcat ccgcgccgcc ttgggtttga agggcggcgg caatttgacg gatggcttcg
    13021 gcattggctt cggcaacaag gcgcaaggat tccgcttcac ctttggcgcg gttgatgcgg
    13081 gcgattttct cggcatttga cgcattgacc gcagcctgag cctcgccttc ggattgttgg
    13141 atttcggctt cgcgctgacc actggcaagg ttgatttgtt cgattttacg accttcggat
    13201 tcggcgatac gggcgcgttt ttcgcgttcg gcagtaattt gcgcctgcat tgagcgaagg
    13261 atttcttgcg gcggaaccaa gtctttaatc tcataacgca aaaccttcac accccaagcc
    13321 ccggccgcct cgtccaaagc cgcaacaaca gtactgttga tttcgtcgcg ttcttcaaac
    13381 gttttgtcca actccatacg cccgataacg gaacgcagcg tcgtttgggc aagctgggta
    13441 attgccatga tgtagttgct cgaaccgtat gaggcgagtt tggggtcggt tacttggaaa
    13501 tagatgatgc cgtcaacagt cagctgcgta ttgtcgcgcg tgatgcagac ctggctgggt
    13561 acgtctaaag ggatttcttt cagcgaatgg cggtaggcaa cgcggtcgat aaagggaatc
    13621 aaaatattca aaccggccgt cagggcgcga tggaaacgcc cgagcctttc gacaacgtgg
    13681 acttcctgtt gtgggatgac aacaaaggat ttgaaaccga aaacggcaac ggctgccaac
    13741 aagataatga aaaattccat aattcctccg agtgttaagg ttttgcgatg ataagaaggt
    13801 tgccttcctt gcggacgatt agggcgcgcg ttcctggttc aagctcttct tgccccgtat
    13861 tttgcgcctg ccagtgcgta ccgcgataaa aaacttcgta acggttgccg cctgtgtgtc
    13921 ggaggatttc gacatattgt ccggcatcca aatcctgata tgaatccgtt tcaacttttc
    13981 taacggcggt tttggcgtgt acgaaccaaa tacccagcgc ggaaagcaga gcggcggtca
    14041 agacggcggc aggcgtgctg ccggtcagcc cgtaagcaat gcccgaaccc gccaaagccg
    14101 cgctgacaac caaaagataa accgttcccg tcaataattc gatgattaag acggcaacag
    14161 cggcaacaaa ccatacagtc atacatttcc ccacaaagcg cgtcgtttga caaaataacg
    14221 caatatcagc agtatagccg aatttgaaag gatagggcag atatggacac ttggcacgat
    14281 gcactcggcg gcgaaaaaca gcagccgtat tttcaggaaa ttttaaatgc agtcaggcag
    14341 gaacgtttgt cgggacaaat catctatccg ccggcggcgg atgtgttcaa cgcattccgc
    14401 ctgacagcgt tcgaccgggt caaagccgtc attctcggac aagatccgta tcacggggca
    14461 gggcaggcgc acggtttggc attttccgtc cggcagggta tccgcatacc gccgtcttta
    14521 ctcaatatct acaaagaatt ggaaaccgac atcgaaggct tttccatacc cgcgcacggc
    14581 tgcctgacag cgtgggcgga gcagggcgta ttacttctga acacggtttt gacggtgcgt
    14641 gcaggacagg cgcattcgca cgccctttta ggctgggaac gctttaccga taccgtcatc
    14701 aggcagcttg cgacacaccg caagcatctt gtctttatgc tgtggggtgg gtatgcacaa
    14761 caaaaaagga aactgataga cagtcaaaac catttgatat tgaccgcacc gcatccgtct
    14821 cctctgtcgg catatcgcgg ttttttcggc tgccgccatt tttcacaggc aaacagctat
    14881 ttgagccggc acggtatcga tccgataaac tggaagctgt gaatgccatc tgaatgccaa
    14941 gttgcttaaa ccccactgaa ggctgctttt gaaaccggaa acaaagtgat taaggaaaac
    15001 cggcccataa tgcaaaaccg tctgaatcct agggttcaga cggcattttt tgccgccatt
    15061 tccaaaaaca acttttgttc gcctgccgac aaatccgaca aagtcgtttg gttgagggct
    15121 ttggcaataa tttaacataa tatacattat gcgaactatc ggaaacagtt gtacgtgtcc
    15181 ctgtggtctt tccaagtagg aaaattaaag tatggacaat gcggctgaat gtatagcccg
    15241 gagcatccgc gtatccgaag gtactgaacg atatgccctg catccctttc gatgcttcca
    15301 tccagccggt attgggaaat acgttgacct tcaatataat atacaggcag ccgacacaca
    15361 ggatatgccg ctgctttttc atcatttctt ctgtcaaatc cttggaacag ttgatttgaa
    15421 aacacggctt tacatacgtc ccaattctct ttgcagtgct tggatatttt gctgcctgtc
    15481 caatacattg ctttgcaatg cgttgatttc ttgatggttg atgttgccgc cctttgccag
    15541 acgtgcttgt gataacattt tttgggcttc aaccaatgct ttgcgttcgt tgctcaattc
    15601 tgtttcgaga atggagcgtc tgctgttgtt tgagggtgct tgttgcggcg gcggcgtatt
    15661 ggattttgcc ggcttggata ctgttttgac cggggcttta tatttgacaa cctgtccgcc
    15721 gtttgacggt gatgataccg gttcgggcgt ttggggcggg atatagcgtt cgctgctgta
    15781 gttgccgatt gggggcaaat cggttgagtg gcagcttttg gacggcttgg tggtgtaaac
    15841 ggtttctccg ttgattgtgc aggtgtagat tttggccgca ttcgcaccca atgggcttga
    15901 aatcagggaa aagttgatta ggattaaggg gagttttgat ttcataatgt gcttgatgct
    15961 cggataatta tttgattttt tggtattttt tataatgctt tcttggcgtt ttacgggctt
    16021 ttcttttcgg ttgtttgctt ttgattttct ttcggggctt tggcagcctg cttgttcttt
    16081 ttcccggcag gttttttgtt tttcttttta ctttctatgt gtattttggc ttcttaactg
    16141 agttttttaa ttttcaggcg gtatccgcct ccctgatggc tgctgatttt aggtaaatcc
    16201 gcatcggagc acgatcctgc tggggctgat gtatatacgc ttttgccatt tgagttgcaa
    16261 tggtatacgg aggcttgcgc cgccgcgccg gaaagggaca ggagacccaa ggcggcaaaa
    16321 agtctgatgt ttataccggt aaaaggcggt gataacgccc gaattatacc gttattggca
    16381 ggcaaagata agcaccctgc ccgcgcttct ttatcgctcg gcaaactgtt tctcgatttc
    16441 agtttcaatc cgttccaatt cttcttgaca agccagccaa acctcttcga tttgggcaag
    16501 ttgtgttttg acttttgcca gctgggataa ggtattttgc aatttttctt tgtttgcttc
    16561 aaggtaagcg tcttcttgtg ctaaaaatgc ttcacatgcc gtctgaattt cggaaagctg
    16621 cgccatttct ttttcggcac ggtctatttt ctgctgtatc ggcttgccgc gtcgggcttt
    16681 ttcctgacgg atttgcgctt cgatgcgctt ggtgtctttg cggctttggc tttgtgcgga
    16741 tgctgcgggt gcggcggcgg cgttttcctg tgccaaacgc cattggcggt aatcgttcaa
    16801 atcgccgtcg aagttcttca gacggccttt gtcgatcagg aggaagctgt cggtagtcgc
    16861 ttcaagcagg ctgcggtcgt gcgatacgat gattaaggcg ccttggaaac tttgcagagc
    16921 gagcgtcagg gcgtggcgca tatccaagtc cagatggttg gttggttcgt caagcagcag
    16981 caggttcggc ttttgccaaa taatcatggc aagggcgaga cgggcttttt ctccgccgga
    17041 aaaaggttcg gttttctgca acgccatatc gccaacaaaa ttgaagccgc cgaggaaatt
    17101 tcggatttct tgttcgcgta cttcgggaga aagctgctga atatgccaaa cagggttttg
    17161 gtcggcgcgg atggtatcga gttggtgttg ggcaaaatag ccgatattga gtttttcgga
    17221 acggacgatg ctgccggaaa gcaaatcgat tttgcccgcc aaggctttga taaacgtaga
    17281 tttaccgctg ccgttgacac ccaatagccc gtagcgtgcg ccgctttcca gcgacagggt
    17341 aatgtcatgc agaacggttt ttccttcgta acccaaatca gcgtgttcta gctttaacaa
    17401 aggattgggc agatggtcgg gatggtaaaa ctcaaaggaa aactcgctgt ccagatgcgc
    17461 gggcgcaacg cgttctagct ttgccaaagc cttcatgcgg ctttgcgctt gaacggcttt
    17521 ggtggctttg gctttgaagc ggtcgataaa ggattgcaaa tgtttgattt gcgcctgctg
    17581 tttgacatag gcagcttgtt gttgcgcgag acgctgcgca cgttcgtttt ggtaaaaatc
    17641 gtaattgccg ccgtattgcg tgagtttttg ctgcgataat tcaatggttt gggtagttgc
    17701 cgcgttgaga aaatcgcggt catgggaaat gatgatttgc gtgcagggta aagaagcaag
    17761 gtggttttcc agccacaaga cggtttccaa atccaagtgg ttggtcggtt cgtcaagcaa
    17821 gagcaaatcg gcgcggcaaa tcagggcttg cgcaagattc aggcgcatac gccagccgcc
    17881 ggaaaaggat ttgacggggc ggctgtgttc ttcttgcgaa aaacccagcc cgttcaacaa
    17941 ttttgccgcg cgggccggcg cggtataagc gtcgatttct tccaatttag catgatattc
    18001 cgcctgcttc atgccgtcat tttgcgcttc cgcctgcctc aatgccgtct gaaaagcctg
    18061 caactcggca tcgccctgca aaacgtaatc caaagcggaa atatccaaat cgggcgtttc
    18121 ttgggaaacg gaagcgagcc gccagttttt cggaatcgag acatcgccgc cgtcctgagt
    18181 gatttcaccc ttgagtaagg caaacaggct ggatttgccc gtcccgtttt tgccgatcaa
    18241 accgacgcgc tgaccgggat tgacagtggc gttggctttg tcgagcagga ttttcaaacc
    18301 gcgttgcagg gtgaggtttt tgatttcaat cataacggaa acatcgtcgg gcggaaaaag
    18361 cccgtatttt acctgaaaat cagtgccgat gccgtctgaa acgggaaatt tacggctgaa
    18421 gccaagccca agccctgcgc ccttccgagt gcaggaaaac caatgtcctg aatgccgaat
    18481 cggtattcat gcattccacg ctgattccga ttcgggaaaa atccgccatg attttgggat
    18541 ggataaactc ctgagccgcg cccgtcccga taatcaatat ttccggatag tcaacaggtt
    18601 tgatgtcgga caacaggttt tccggagtca gatcggacaa ggttcggcat tgcgacaggc
    18661 agaccgaatc cttatgtaca agcacgggtt tatggaaact ttgccccgcc agccggattc
    18721 cgcccgcacc gcattcatat tccgcaaact gtccgtctat cggattttct tcaaacaaca
    18781 tttttttacc ccgtcgccgc atcatctaca ccgaaagggg tgcaaaatca gacaaattca
    18841 tgtaggattg gcagatttca tctgacccgc ctgccgattt cagacggcat ttgattcaaa
    18901 gtgcggcaca attatatcgg cagcggatat tttcgtcttt caatatttac atttcagtcg
    18961 gtttacaagg agacacaatg aagccagtaa acatcggtct tttaggttta ggtacggtcg
    19021 gcggcggtac ggctgccgtg ttacgggaca atgcggagga aatttcccgc cgtttgggac
    19081 gcgaagtgcg catcagtgcc gtatgcgact tgagcgaaga aaaagcccgc caaatctgcc
    19141 cgtccgcagc ctttgtcaaa gacccgttcg aactggtcgc acgtgaagac gtcgatgtag
    19201 tcgtcgaatt gttcggcggt accggcattg ccaaagatgc ggtgttgaaa gccattgaaa
    19261 acggcaaaca catcgttacc gccaacaaaa aactgctcgc cgaatacggc aacgaaatct
    19321 tcccgctggc ggaaaaacaa aacgtcatcg tccaatttga agcggcagta gcgggcggta
    19381 tcccaatcat caaagccctg cgcgaaggtt tggcggcaaa caggattaaa tccatcgccg
    19441 gcattattaa cggcaccagc aacttcatcc tctccgaaat gcgtgaaaaa ggcagcgcgt
    19501 ttgccgatgt actgaaagaa gcgcaggcac tgggctatgc cgaagccgac ccgaccttcg
    19561 acatcgaagg acatgacgcg ggccataaaa tcaccatcat gagcgcactg gcattcggca
    19621 cgccgatgaa cttttccgcc tgctacctcg aaggcatcag caaactcgac agccgcgaca
    19681 tcaaatacgc cgaagaactt ggctatcgca tcaaactgtt gggcattacc cgcaaaaccg
    19741 gcaaaggcat cgagctgcgc gtccacccta ccctgattcc cgaaagccgc ctcttggcaa
    19801 acgtcaacgg cgtgatgaac gccgtgcgcg tcaacgccga tatggttggc gaaaccttat
    19861 attacggcgc gggcgcgggc gcattgccga ccgcttccgc cgtggttgcc gatatcatcg
    19921 acatcgcccg cctggttgaa gccgataccg cccaccgcgt accgcatctg gcgttccaac
    19981 ccgcgcaagt ccaagcgcaa accatcctgc ctatggacga aattaccagc agctactacc
    20041 tgcgcgtcca agccaaagac gaaccgggca cgctggggca aatcgccgcg ctgttggcac
    20101 aagaaaacgt gtccatcgaa gcactgattc aaaaaggcgt gattgatcag accactgccg
    20161 aaatcgtgat tctgacccac agcacggtcg aaaaacacat caagtcggca atcgcagcca
    20221 tcgaagcact ggattgcgtg gaaaaaccga ttaccatgat ccgcatggaa agcctgcatg
    20281 actgagccga aacacgaaat gctgacgaaa gagcaggttg ccgcgcgcaa aaaagcaaaa
    20341 gccaaaatcc gcaccatccg catttgggcg tgggtcattt tggcgttgct cgctttaacc
    20401 gccctgctct cccaatgcgc gatgtccaaa ccgcaggcaa aacagaaaat tgtcgagtct
    20461 tgcgtgaaga atattccgtt tgccgaaaaa tggcaaaacg atttgcgggc ccgcggttta
    20521 gattcaaaca atacccgcct cgccgtcgac tactgcaaat gtatgtggga gcagcctttg
    20581 gacagattga gcgagaaaca gattagatcc ttcggcaaac tcggcgcaca agaacagctt
    20641 gacctgctcg gcggcgcaaa tgcctttgaa gcacgtgaca agcagtgtgt tgccgatttg
    20701 aaatcagaat aatgtggacc gataaaaaag ccgattcttt aaagaatcgg cttttttcat
    20761 aaaaaacggc ttacagtgcg tctttcaaag ctttgccggc gcggaattta ggcgttttgg
    20821 cggcggcaat ggtcagaggc tcgccggttt tggggttgcg gccttggcgt tccgcacgtt
    20881 cgcccacgta gaaagtaccg aaaccgacca aagtaacggt gtcgccttgt ttcagggcgg
    20941 tggttactgc attggtagtg gcatccaaag ctttttgtgc ggcggctttg gaaatgtcgg
    21001 cttcttgagc aatcgcttcg atcaattcag acttgttcac aatcagtccc ttcctgtctt
    21061 aaaaaatgat gaaatgcccg aatactcggg gtttgtactg cttgagcaac tttcgcttta
    21121 tagcaattct gaaattgccg tgtcaagcaa aaaatacgga atcaccctat ttgacaggct
    21181 ttcaggacga aaccgcattt ttacaacaca tttcctgcgt ttcaatgttt ggttgccctg
    21241 ctgcggggtt ttggttttga agcggattcc gccgccgctt ccgcaccaga aggttctgcc
    21301 caaggctcag gctggctttc caaacccaga gccaatacct cgtctatcca tttgaccgga
    21361 tggatggtca ggccggtttt cacgttttca gggatttctt ccaagtcttt gaggttgtct
    21421 tttggaatta aaacgtgttt gatgccgccg cgaagcgcag ccaacagttt ttccttcaga
    21481 ccgccaatcg gcaggacttc gccgcgcagg gtaatttcgc ccgtcatcgc cacatcggcg
    21541 cgcaccggga ttttggtgaa tgcagacacc atcgccaaag tcatggcaat gcccgcactc
    21601 ggaccgtctt tcggcgtcgc accttcggga acatggacat ggatgtcttt tttctcgtaa
    21661 aaatcaggag ccaagcccac tgattccgcg cgggagcgga caaccgacca cgctgcagac
    21721 acggattcct tcatcacatc gcccaactgg ccggtgcact gaatcacgcc cttacccagc
    21781 aatgctgcgg cttcgacggt cagcaattcg ccgccgactt ccgtccacgc caaaccggtt
    21841 acttgtccga tacggttttc gctttcggca acgccgtaat cgaagcggcg cacacccaaa
    21901 tagtcgtgca gatttttctc atttacttta accgccctgg gcttggcttt tccggttttt
    21961 ttggtttttg agtcttcgtc caaggtaatc tgcatcacca ccttgcggca gattttggca
    22021 atctcgcggt cgagcgaacg cacgcccgcc tcgcgggtgt agtaacggat aatatcgcgc
    22081 accgcgcttt cttcgattgc caattcccct tcttttacgc cgttgcgctt catttgcttc
    22141 ggcacgaggt actgcatcgc gatattgatt ttttcgtctt cggtatagcc ggacagacgg
    22201 atgatttcca tacggtcgag cagcggtgtc gggatattca ggctgtttga agtcgcaata
    22261 aacatcacat cgctcaaatc gtaatccact tcggcataat gatcggcgaa cttgttgttt
    22321 tgctcgggat caagcacttc gagcaacgcg ctggcgggat cgccccggaa gtcgctaccc
    22381 aatttgtcga tttcgtcgag caggaacaat gggtttttta cgccggcttt tgccatattc
    22441 tgcaaaatct taccgggcat ggagccgata taggtgcggc ggtgtccccg gatttcgctt
    22501 tcgtcgcgca cgccgcccaa agccatgcgg acatatttcc gccccgttgc tttggcgatg
    22561 gattcgccca aagaggtttt gcccacgcct ggagggccga ccaggcacag aatcgggcct
    22621 ttgagtttgt ccatacgttt ttggacggcg aggtattcca aaatccgttc tttgactttt
    22681 tccaggccgt agtggtcggt atccagcacc aatccggctt tggcgatgtc tttgctgacg
    22741 cgggattttt tcttccacgg cagttcgagc agggtgtcga tgtagttgcg tacgacggtg
    22801 gattcggcag acatcggcgg catcattttg agttttttca gttcggacag gcatttttct
    22861 tccgcttctt tgctcatccc cgcctttttg atgcctgctt ccaaggcatc cagttcgccg
    22921 ttttcgtctt cttcgcccaa ttctttgtgt atcgctttaa tctgttcgtt cagataatat
    22981 tcgcgctggg atttttccat ttggcgtttg acgcgtccgc gtatgcgttt ttcggcctgc
    23041 ataatgtcga gttcggattc cagctgtgcc agcaggaatt ccatccgttt gccgatttcg
    23101 ggaatttcca aaatctgttg gcgttgcgcc agtttcaact gcaaatgcgc tgcgaccgta
    23161 tcggttagcc ggctgttttc ggcaatgccg ttaatgctgc cgataatttc ggcggggatt
    23221 tttttattga gtttggcgta ttgttcaaac tgcgccaaca gggtgcggcg cacggcttcg
    23281 aggtcggtat tgccgcccgt gtcttcttcc acgaccgtct ctatatggga aacgaacaga
    23341 ccgcccgtgt cttcaatggt cagaacacgt ccgcgataca gcccttcgac caatactttt
    23401 accgtgccgt cgggcagttt caacacttgc aggacttgtg cgaccgtacc ggtctgatac
    23461 aggtcggcgg caaccggttc ttctaccgcc gcatcggttt gcgccaacag gaaaaccggc
    23521 tcctcgcggg taatggcgtt ttccagtgcg gcgatggatt tcggcctgcc gacaaacagc
    23581 ggcagaacca tatgcgggta aacgacgaca tcccgcaaag gaagggttgc caaggcggca
    23641 tattcctcaa aatgcttttc tttttgtgtc ataggtactc tcttgtgtct gacagattgc
    23701 cgattttcgc gtacattggg gttgaaggta ttatttcaag catatgtggt ttatttatgg
    23761 agtttgatgc gatgccgtct gaaacattcc ggcttcagac ggcatgggct tggaaagaca
    23821 aggcgggaac aaaaaactgt tctgtgttgc cgctccttgc tgtaccatcc gtatggtttg
    23881 cggttctgcc gccctatttt caaaacatga cgcaggtatt ccatgtcttc ttttattttt
    23941 cccgataacg ggttcgaatg gcgcaacgaa agtctgcaaa agccgcccaa aggttggcat
    24001 tatgtccgcg aaagtggtgc aaacggcatt ttgaaggctg cctatcaaaa tattgcaact
    24061 gtctggcagg gcgatttcca caatgccaaa caggtgcttt ccgcaatgaa gaagaaggtc
    24121 cgcaaacctg ccgccgtccg ttccgatgcg gatattgccg cccttttcca cgcccaccgt
    24181 atgaagcagg cgcagcagag ccgtattctg aatatgcttg ccgttgaaat ccgccccggt
    24241 tttgtgttgg acaacaaacg cgcgcccgat atacgcgccg ccctgctcga cgtgtacgga
    24301 gaggcggacg gcaaaccgtt tttcctgccg ctcaatctgc tgctggggtt tatgggtgcg
    24361 cacgagtggc ataagaaagg ggttgccgtt ccgcagctgg gcggcagcat acacgttcct
    24421 ttcggcgtat tctcgccgtt gcgcggcgaa tacctcgacc tgctcgccca tgcgccgtca
    24481 acgggttttc agacggcatt cgatatcggg acgggctcgg gcgtgcttgc cgccattttg
    24541 gcgaaacagg gcattccttc cgtcatcggc acggatacca atccgaaagc cgtcgcctgc
    24601 gcccgtgcca atattgcccg tttgggcttt gaaaaacagg ttgagatacg ggaaaccgat
    24661 ctgtttcccg aagggtttgc cgacctgatt gtctgcaatc cgccctggct tcctgccaag
    24721 ccaacttccg ccgtcgaatc cgcgttatac gaccccgaat ctgcgatgct ggctgcgttt
    24781 ttgcgggatg cgccgaaaca tctgaatccc gatggagaaa tccgtctgat tatttccgac
    24841 cttgccgaac atctgcacct gcgtccgtcc gattttctgg ataaggcatt tgctcaggca
    24901 gatttgcgcg ttgcggatgt tttgaaaacc aaaccggttc acaaaaaagc tgccgacccg
    24961 gacgatccgc tggcttttgc ccgaaacagg gaaactactt tcctataccg tttgaaaaag
    25021 gcataagggg ctgcggtgcg cttccggcgg caggcatcgg aacgcccgcg ccgccggcac
    25081 ggcatcattc gggcggatta ttcttgtgaa aatacccgct cgagcatact gcccaatgcc
    25141 gtctgacgcg ttttgacggt ggcggcagct tgccggaagg cttcttcccc gccgaagaat
    25201 acgaacttct ctctcgcgcg ggtaatggcg gtatataaca gctccttact caatccggac
    25261 aatgcatcgt ccccttcgtc cgaaggtgcg gcggaaggcg gcagcagcca gacttcccgg
    25321 tattccgaac cttggctttt gtggacggtc atggcgaatg cgggttcaaa ttcgggcagg
    25381 cagcttaccg ctaccttttt aaatccgtcc gcatcggcaa aataggcggc aaggctgccc
    25441 tgccgtccga catcttccat aatcagtccg atgtcgccgt tgaacagttc aagcgcgtag
    25501 tcgttctgcc tgatcataat cggctctccg gcaaaatatg ccaaatgttc cggtatgttc
    25561 attttgcggc gtacatggcg gcaataggct tcgttgaagt cttccgcatc ctgccgccaa
    25621 gctgccagaa ccacgatatc cgaaatgccc gcgtatgcgg cttcgatatt gccgtctttt
    25681 accgcctgcc aataggcttt gtgtgcccgg tacaaccttt cgactcgagc gttcggactg
    25741 cattccgaat gttccagttc gtccggaaac cggtcaaaca atgcccacgc cccttcatcg
    25801 cccgatacgg cggcacgggc aaggcagccg atgccgctgt tgtcgccgaa gcggtggctg
    25861 aacgacagat gggcggtgtt ttgcgccaac acgggtggat ttgcgctgac gctgaaaccg
    25921 tgttccggaa ggaagccggc cagcctttgg tgcgtttctc cgtccaaaac ggttttttgt
    25981 gacaaaacgg acagcaccgc ccctattccg acggacggga gctggttttc atcccccagc
    26041 agaatcacgc gcgcgccggt tttgaccgct tttaaaagtt gcagcatcaa tgccgtatcc
    26101 agcatagagg cttcatcgat aaccaatacg tcaaacggca gcgggtaaac agggttgaac
    26161 gccgcctgca ttttgggcgg gcgcagcttc agcagtcggt ggacggtttg cccttccagt
    26221 ttgagcaaat ggcggcggac ggcctccggc gcgtcaaaac cgttgattgc acggtgcagc
    26281 gcgcgcgcca tatgtgccgc cgctttgccc gtcggtgcgg caagcgcgat gttgggaaga
    26341 ttttcgtctt caccgcaaat cagcgccagc agtttggcaa ccgtcgtcgt tttacccgtc
    26401 ccgggtccgc cggtaatcac cataaaaggc tgcaacagtg ccaaggcggc ggcatcgcgc
    26461 tgcccttcgc tgcccgtgcc ttgaaaccat tttgcgaggt tttgccgcgc gcctgccgcg
    26521 tcgggggcgg acgtgtcggc tgccgccaag cgttttatct cggcagccaa atcgtattcc
    26581 aactgccaca tcctgcccaa aaacagcctt ctgccttcca aaatcaaagg cgcggcggat
    26641 gttccgacca cgggtgcgag tgccgacagc gcgtcagcct cgccaccgct caaacggata
    26701 aacgaatgac cgttttgcaa tgcctgaaac aggcgttcgg tgcagtttgc aagcacttcg
    26761 ctgcctgaac ccgcatagcg tgccaaaaac cagattgccg cccttgccgc cgcttgggca
    26821 aattcatctg tctgccgttc cattgtattc cttgtcgaaa tgccgtctga aggcgtgggg
    26881 cttcagacgg cgcggtgttg tttcggctgt ttaggcgttt gcgccctgtt tggggtgcag
    26941 gctgccgtct ttcatgacca tcacgcgctc gaagcggccg gcgagttcgt cgtcgtgcgt
    27001 tacgaccacc agccccgttc ccaattccgt tttcagttcc agcatcatat ccaaaacatt
    27061 cctggcgttc gcacggtcga ggttgccggt cggttcgtcg gcaagcaggc attttggttg
    27121 cgtaaccaag gcgcgcgcaa tggcggcacg ctggcgttcg ccgccggaca gttcgcccgc
    27181 gcggtgcgtc gaacggtgtt tcagtccgac cttttccagc atcgccattg ccgtttctgc
    27241 tgcctcttca cggctttttt tgccgatcag aagcggcatc atcacgtttt ccagtgcgga
    27301 aaactccggc agaagatggt ggaactggta cacgaaaccg agatggcggt tgcgcaggat
    27361 gcccagttgt cgctggctta atgtacgcaa atcctcgccc atcagcagca acctgccttc
    27421 agacggcata tccagcccgc ccagaatatg cagcagcgtc gattttccgc tgcccgaaga
    27481 accgatgatg ccggtgcttt cccccgcgcg gatttccaaa tccaagccgt gcagcacccg
    27541 aacgtccaaa ccgccgtcgc ggtagcgttt gcccacgcct tcgcatttca aaatcaactc
    27601 actcataacg caaagcctcc gccggttggg tttttgaggc acgccagctc gggtagagcg
    27661 tggcaacgaa agacagtccc aaggaaatgc aggcaatcag ggcaacgtcg cccatatcga
    27721 catcgctggg caggtagtcg ataaaataaa cctgcgaatt gataaggtgg acaccgagca
    27781 ggttttcaaa aaacgccacg accctgccga cgttccaacc caaaagcacg ccgcagacca
    27841 cacccgccag cgtgccgaaa aagcctgaaa acgcgccctg caccataaaa atcttcatca
    27901 cgccgccagg ggaaagaccc aaagtccgca aaatcgcaat gtccgcctgc ttttccgtaa
    27961 ccgccatcac caaggaagag acaaggttga acgccgccac ggcgataatc agcgtcagga
    28021 tgatgaacat catccgtttt tccaactcga ccgcctcaaa atagctgcgg ttgttgtacg
    28081 tccaatcgcg cacccaaacc gcgtcccttt gcgcctccgg aatcagtgtt gccgtcaagg
    28141 cgggggcgtt ttgcgggtcg gcgagtttca gccgcagccc cgcaacttcc ttatccaagc
    28201 ggtacagtac gcgcgcgtct tggatatgcg tcatcgccaa tgaattgtca acctcgtaaa
    28261 cacccgtttt aaccagaccg accacggtaa actgtttcaa cctcggtacg actccggcag
    28321 gggtaacgtt gccctccggc gtgatgacgg taactttatt gccgacttcc gcccccaaag
    28381 cctccgccaa gccgacaccg aggataatgt caaactcgcc cggaatcaga tcttcaaatt
    28441 tgcctgccgg cattttgtcg ccgtattcca ccactttgcg ctcttcagac ggcaaaatgc
    28501 cgcgcatctg cacgcccctg atttcgcccg cgtttgccag cagagcctga ttggaaacat
    28561 agggcgcggc agccaaaata cctttgcggt tttcggtaaa ccgaagcagg ttgcgccaat
    28621 ccgtatccgt attgtcgata tagccgattt cggcgtgcgg cgcgacattc aggagctgcc
    28681 cgcgtatttc tttctgaaag ccgttcataa ccgacaagac gacaatcagc gcggttacgc
    28741 ccaaggcgat tccggcaatc gaaaccatcg tgataaacga cataaagccg ttgcgctttt
    28801 tcgccctgag atacctcaag cctatccaag cctctagaga aaacataacg ctaccttaaa
    28861 aatgtctgca aacgtgccgc cccggacggc ggtttggggg cggcggcaaa agtttattgt
    28921 accgtaaaac ccaggcagcg tccgaacgcc cgagtgcggg ggacggggcg gcagagcggg
    28981 cggaaaactt gcacaacgcg tcaaactgcc ctatcctttc cttcagaaaa accgtttctt
    29041 gaggaaaaca atgaatatcc gaactgcttt tgctttgtgc gccatcgcct tatccgccgc
    29101 tgcggctgcc tacgccaaag aaatcaaaat cgatgccaac aacacgcctt attccgaagc
    29161 cgacgcgcaa aagctggcgg caacggcagt cggtatgggt gttaaggaac ctatcagcct
    29221 gaacggcggc agcgacagca ttaccgtgtc cggcagcagc gcgacgcagt gcgtgttcaa
    29281 agtcggcaac ggaggcgcat tgcagattca agggctaaac tgcaagtaaa ccgcccggaa
    29341 aaatgccgtc tgaaggcttc agacggcatt ttgcattggc ggcgttatgc cccgccttct
    29401 ttaatcaggc ggcgttcgta caccgcctgc gccagcgttc ccgcatcgac atattccaat
    29461 tcgccgccca agggaatgcc ctgcgacagc ctgctgactt tgtaaggcag gtttttaaaa
    29521 aactcggaca ggacatacgc cgtcgcattg ccttctgcgg taaaagcggt tgcaataatg
    29581 atttcttcga cttccccgcc gcccagccgt tgcgccagcc tgtccaatgc gatggcggat
    29641 acgtccattc ccaatgccgt attgatttgc cccatcagga cgaaatacag cccgtcgtgg
    29701 cagtttgccg cttccatatt cgacacgtcg gcaggcatat gcaccaccat cagccgccgc
    29761 ccgtcgcgtg tttcatcggc acaaatatcg cacaatccgc cttcgcaaaa cgtgttgcac
    29821 atcgcgcaat ggtaaacctg cttcaatgcc gtctgcaagg catccaccag tttttcagcc
    29881 tctttgcgct tgtgttggag caaatgatac gctatccgct gtgccgattt cggcccgacg
    29941 ttgggtaaaa ccttcagcgc gtcgatcaat ccttggaagg catcttgttt tttgtggctc
    30001 atcatattcc gccgtatggg aaaacggccg gaatattccg accgttattt tgtcaacaaa
    30061 agtgtcaatt actgaccgtc gccgttgtcg accgattgcg ctcctttggt ctgtttgatt
    30121 ttgccgttga aataacgtat caacaagtcg aaagtattgg cagactgctg ttgcgccaaa
    30181 gcctgttttg caagcggaag ctgtgcggcg atatcatccg gcggggttac agcctgtact
    30241 tcgacaatca cgggtgccgg cagaccgatc agcctgacgt aggcgggttt gccgtttgcc
    30301 ggttttgctt tcagcagttc cgcataagcc tcgggcggca tggactgcct tgcctgctgt
    30361 gcgcccaaaa cggacacttc cgaccatttc acgtcaacag ccttgccgcc gttcagttgg
    30421 gtaagcacgt cttttgcctt gttttcggca agtttggcgg cttcggtacg gatataagcc
    30481 tgacgtaccg cgtctttggc ttcggcaaac ggcagggttt tctcttcgcg gacttctttg
    30541 gcgcggacga cccacgcggt ttcgctgttg atggtcagca cttcggaatt gtgttttttc
    30601 ttcaatacgt cgtcgctgaa aacggcattg atcaggtttt cgggcatacc ggacatttgc
    30661 gcatcctgcc tgctcagcca agtttcttgg gtttcgactt tcaaaccgct gtttttggcg
    30721 gcttcggcaa gcgaggaagg atggttgaac gcgtcatcgc ccagcttttc ttttgcctta
    30781 ttgaagtcgg caaccgcctt tttcattttc aattcgtttt ctacggcggc tttttcctgc
    30841 tcgaaagaag gtttggcttc atttgccggc aaacgcgcca cgcgctcttc aaacgcattt
    30901 ttcacttctg tttcgctgac ggtctgtttg tctgcaaagt ctttcagatt caaggcgaca
    30961 tattccaatt tgaccgcttt gggaagcagg tagtcttttt tgtttgcgtt ataaaacttc
    31021 tgcaaatcgg cttcagacac tttgacttgg gcgatgaatt cgtcggggtt gaaagtgtgc
    31081 gaacggatgg tgcggttgac ctgcgtcagc ctgatcagct gttccgcctg cgcgtcgccg
    31141 accaatacgc cgttttggac gaggtttacc aaattctgca aggcaaactg atcgcggatt
    31201 tcttcgacaa actggtcttc agacatatga cgttgggaaa ggtagcggtt taaaagcgcg
    31261 tggtcgaatt tgccgtttgc gtcgtggaaa ttgggatcgt ccacgataat ctgcttgatt
    31321 tgttcggaag aaaccgaaat gcccatcagc ttcgcgccct gtttcaggta ggcgcgttgc
    31381 agcagggatt ggaacaccgc gtcgcgcgaa gggctgccgc cgtccgcctg ttcattttgc
    31441 atagcgttgt tgatggagtg gtcgctgatt ttttcgtcgc ccacttggac gatgtagtcg
    31501 gcacccggat gggataccgt gctgaccccg aagccgacga aggttaatgc aatcagcccc
    31561 aaaaggactt gggcgggcgt tctgtatttt tcgatggaat ggaacatatt ttcagattgg
    31621 aaatgggatt gggaaattca agtcgggtat tgtaacggtt tttatccctg tctgcacggg
    31681 gcttgccgct tgaagatgcc gtcgtaggtt tcttcgctga agccgacata aaccctgccg
    31741 ccgcactcca atacgggacg cttgatcagg ctcggcattt cggacatcag tttgacggcc
    31801 tccgccgtcg aggacagcac tttttgctgt gttccggcat caagtttgcg ccagctcgtc
    31861 ccgcgtttgt tgagcagggt tgccaaaggc acttgttcca gccacgagca gatttccgct
    31921 tcagacggcc tctgtttttt aaaatcccga aattcaaact ccaagccgta tccggcaagc
    31981 ctgtttttgg cttttttgac tgtgtcgcaa tttggaatgc cgtgaaggat tatcatttgg
    32041 aaaccttttg tctgaaataa taaaacggat attttactat aagtgtctga aaatttgccc
    32101 gtctgtttca gacggcgggg cggttatgtt acaatccgaa aattcgaaaa atttaatctc
    32161 ttgttcaata aaggctttac caatcatgat ttctaccaac ggcatcacca tgcagttcgg
    32221 cgcgaagccg ctgtttgaaa acgtatccgt aaaattcgga gaaggcaacc gttacggcct
    32281 gatcggcgcg aacggttcag gcaaatccac cttcatgaaa atcctcggcg gcgatttgga
    32341 acagacggcc ggcgaagtgg cgattgaaaa cggcgtgcgt ttgggtaaat tgcgccaaga
    32401 ccagtttgcc tacgaagaca tgcgcgtgct ggacgtggtg atgatggggc ataccgaaat
    32461 gtgggcggcg atgaccgaac gtgatgcgat ttacgccaat cccgaagcca ccgaagacga
    32521 ctacatgaaa gccgccgaac tggaagccaa gttcgccgaa tacgacggct acaccgccga
    32581 agcgcgtgcc gccgaactgt tgagcggcgt gggcatttcc gaagatttgc acaatgcgaa
    32641 aatggcggaa gtcgccccgg gcttcaaact gcgcgtattg ctggcgcagg cgctgttctc
    32701 caagccggat gtattgctct tggacgaacc gaccaataac ttggacatta ataccatccg
    32761 ctggttggaa ggcgtgttga accaatacga ctccacgatg attatcatca gccacgaccg
    32821 ccactttttg aacgaagtct gcacgcatat ggcggatttg gactacaaca ccatcaccat
    32881 ctatccgggc aactacgacg actacatgct cgcctccgcc caatcgcgcg aacgcgccct
    32941 gaaagacaat gccaaagcca aagagaaact gcaagagctg caagagttcg tcgcccgctt
    33001 ctctgccaac aaatccaaag cccgtcaggc aaccagccgt ctgaaacagg ccgacaaaat
    33061 caaatcggag atggtcgaag tcaaaccttc cacccgtcaa aacccgtata tccgttttga
    33121 agccgatgaa aaagccaagc tgcaccgtca ggctgtggaa gttgaaaaac tggcgaaact
    33181 ctttgaaacc cagttgttta aaaacctgaa cttcatcctt gaagcgggac aacgcctcgc
    33241 catcatcggc ccgaacggcg cgggcaaatc caccctgctg aaactcttgg ccggcgcgta
    33301 caaccccgaa tattcagacg gcctgttacc ggacgaaggc accatcaaat gggcggaaaa
    33361 agccagtgtc ggctactatc cgcaagacca tgaaaacgac ttcgacgtcg atatggacct
    33421 gagcgaatgg atgcgccaat gggggcagga aggcgacgac gaacaagtca tccgcggcac
    33481 tttggggcgt ttgctcttcg gcagtaacga tgtcgtgaaa aaagtgaagg ttctctccgg
    33541 tggtgaaaaa ggccgtatgc tttacggcaa actgttgctg ttgaaaccca atgtcttagt
    33601 catggacgaa ccgaccaacc atatggacat ggaaagcatc gaatccttga acatggcact
    33661 ggaaaaatac aacggcacgc tgatttttgt ctcccacgac cgtcagttcg tttcctcctt
    33721 ggcaacccaa atcatcgaac tggacggcaa aggcggatat gaacactact tgggcgatta
    33781 cgaaagttac ttggagaaaa aaggcgtagc ataaccgccg gttggaacaa tgccgtctga
    33841 agccgcttca gacggcattg ttgataactt taaaatagga agcatatgca gacttatctc
    33901 gtcggcggtg ccgtccgcga ttatcttttg ggcttgcccg tcaaagaccg cgattgggtg
    33961 gtcgtcggcg cagacgcaca aaccatgctg gcgcaaggct tccagcctgt cggcaaagat
    34021 tttcccgtgt ttctccatcc cgaaacgcac gaagaatacg ccctcgcccg caccgagcgc
    34081 aaaaccgcca aaggttacgt cggtttcagt ttccacgccg acaaagacgt tacgctggag
    34141 caggatttga tgcgccgcga cctgaccatc aacgcgatgg cgcaagatgc ggacggcaag
    34201 attatcgacc ctttcggcgg acaacgggat ttggcggcag gcattttgcg ccacgtttcc
    34261 ccggccttcg ccgaagaccc cgtccgcatc ctgcgtactg cccgctttgc cgcgcgttac
    34321 aggtttgaaa tcgccgaaga aaccataaag ctgatgcggc agatggtgga aaacggcgaa
    34381 gcggacgcat tggttgccga acgcgtctgg caggagtttg cgaaaggttt gatggaaaaa
    34441 aatccgcgca aaatgattga agtgttgcgc gaatgcggcg cgctcaaagt cttgctgccc
    34501 gaagtcaatg ccctcttcgg cgtgccgcaa cgcgccgact accatcccga aatcgacagc
    34561 ggcatccata ccctgatgac gctgcaacgc gccgccgata tgggtttgag cctgcccgaa
    34621 cgctatgccg ccctgctgca cgacttgggc aaagccaaaa caccgtccga catcctgccg
    34681 cgccaccacg gacacgacct cgccggtgtc gaacccgtgc gcgaagtcaa tcagcggctg
    34741 cgtgcgccga aacattgcgc cgagcttgcc gaacttgtct gccgttggca cattattttc
    34801 caccaagtcg gacagcttaa aagccaaacc attctgaacg ttttgaaaaa aaccgacgct
    34861 ttcagacgcc ccgaacgctt ctccgctgcc ttaaatgtct gcattgccga tacgcaaggt
    34921 cgtctgaacc gcgaacacac gccctacccg caacgcgcgc actggctcgc cttactcgaa
    34981 gtcgccaatc aagtggattc gggcaaaatc gccgccgaat gccgcgcgca ggggaaagtg
    35041 caccttatcg ccgaacaaat cgaccgggcg cggctggcac aaatcgcccc attgcaaaaa
    35101 gcgtttcgag cggcgcaaga caaaacagaa aaacattaaa acgcccaatg cagccactt
//
LOCUS       DAWFBO010000004        32177 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFBO010000004 DAWFBO010000000
VERSION     DAWFBO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.004"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(379..654)
                     /locus_tag="WGO87_000134"
     CDS             complement(379..654)
                     /locus_tag="WGO87_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0391568.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(651..1934)
                     /locus_tag="WGO87_000135"
     CDS             complement(651..1934)
                     /locus_tag="WGO87_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0391569.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWPEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2090..3508)
                     /gene="glnA"
                     /locus_tag="WGO87_000136"
     CDS             complement(2090..3508)
                     /gene="glnA"
                     /locus_tag="WGO87_000136"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0391570.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3592..3741
                     /locus_tag="WGO87_000137"
     CDS             3592..3741
                     /locus_tag="WGO87_000137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391571.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLLFVCSKAEYPPFCPNAD
                     KTDAV"
     gene            complement(3782..4168)
                     /locus_tag="WGO87_000138"
     CDS             complement(3782..4168)
                     /locus_tag="WGO87_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391572.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVQVGIDSDNNTTFGDADSRTD
                     NRAWFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPS
                     YAEWNYVIPGTLGEAQYKFVCNGYPR"
     gene            4340..5149
                     /gene="aroE"
                     /locus_tag="WGO87_000139"
     CDS             4340..5149
                     /gene="aroE"
                     /locus_tag="WGO87_000139"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0391573.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            5151..5852
                     /gene="mtgA"
                     /locus_tag="WGO87_000140"
     CDS             5151..5852
                     /gene="mtgA"
                     /locus_tag="WGO87_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0391574.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDAHFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(6055..6130)
                     /locus_tag="WGO87_000141"
     tRNA            complement(6055..6130)
                     /locus_tag="WGO87_000141"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6095..6097),aa:Phe,seq:gaa)
     gene            complement(6321..7055)
                     /gene="lptB"
                     /locus_tag="WGO87_000142"
     CDS             complement(6321..7055)
                     /gene="lptB"
                     /locus_tag="WGO87_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0391575.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(7129..7659)
                     /gene="lptA"
                     /locus_tag="WGO87_000143"
     CDS             complement(7129..7659)
                     /gene="lptA"
                     /locus_tag="WGO87_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0391576.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7640..8221)
                     /gene="lptC"
                     /locus_tag="WGO87_000144"
     CDS             complement(7640..8221)
                     /gene="lptC"
                     /locus_tag="WGO87_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0391577.1"
                     /translation="MKVRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPNEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDLPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(8218..8754)
                     /locus_tag="WGO87_000145"
     CDS             complement(8218..8754)
                     /locus_tag="WGO87_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0391578.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLSVAVLDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMRAKGTLGAALNEYIK"
     gene            complement(8819..9793)
                     /locus_tag="WGO87_000146"
     CDS             complement(8819..9793)
                     /locus_tag="WGO87_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0391579.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9889..10944
                     /gene="tal"
                     /locus_tag="WGO87_000147"
     CDS             9889..10944
                     /gene="tal"
                     /locus_tag="WGO87_000147"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0391580.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRASCGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALEAQVANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            11098..11388
                     /locus_tag="WGO87_000148"
     CDS             11098..11388
                     /locus_tag="WGO87_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391581.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            11405..12292
                     /gene="gluQRS"
                     /locus_tag="WGO87_000149"
     CDS             11405..12292
                     /gene="gluQRS"
                     /locus_tag="WGO87_000149"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0391582.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQTGARRGADGFVYNGRCRNPQQRPAPQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMGKVPKHAITTP"
     gene            complement(12362..13372)
                     /gene="dusA"
                     /locus_tag="WGO87_000150"
     CDS             complement(12362..13372)
                     /gene="dusA"
                     /locus_tag="WGO87_000150"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0391583.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQCLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(13403..13894)
                     /locus_tag="WGO87_000151"
     CDS             complement(13403..13894)
                     /locus_tag="WGO87_000151"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0391584.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(13967..14725)
                     /locus_tag="WGO87_000152"
     CDS             complement(13967..14725)
                     /locus_tag="WGO87_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0391585.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSEDELHKFYEQQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(14796..15662)
                     /locus_tag="WGO87_000153"
     CDS             complement(14796..15662)
                     /locus_tag="WGO87_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0391586.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15711..15989)
                     /locus_tag="WGO87_000154"
     CDS             complement(15711..15989)
                     /locus_tag="WGO87_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0391587.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGDMIHALGIRAATPDEYFHTAD"
     gene            complement(15989..16279)
                     /locus_tag="WGO87_000155"
     CDS             complement(15989..16279)
                     /locus_tag="WGO87_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0391588.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(16282..16812)
                     /locus_tag="WGO87_000156"
     CDS             complement(16282..16812)
                     /locus_tag="WGO87_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0391589.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVIFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            17120..19645
                     /locus_tag="WGO87_000157"
     CDS             17120..19645
                     /locus_tag="WGO87_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0391590.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENVTETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTKTVA
                     EIPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENAAEITLE
                     TPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKY
                     DLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(19818..20294)
                     /gene="gloA"
                     /locus_tag="WGO87_000158"
     CDS             complement(19818..20294)
                     /gene="gloA"
                     /locus_tag="WGO87_000158"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0391591.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(20258..21427)
                     /gene="lapB"
                     /locus_tag="WGO87_000159"
     CDS             complement(20258..21427)
                     /gene="lapB"
                     /locus_tag="WGO87_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0391592.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(21438..21752)
                     /locus_tag="WGO87_000160"
     CDS             complement(21438..21752)
                     /locus_tag="WGO87_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0391593.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            22062..23060
                     /gene="ilvE"
                     /locus_tag="WGO87_000161"
     CDS             22062..23060
                     /gene="ilvE"
                     /locus_tag="WGO87_000161"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0391594.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLVPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(23245..24030)
                     /gene="fabI"
                     /locus_tag="WGO87_000162"
     CDS             complement(23245..24030)
                     /gene="fabI"
                     /locus_tag="WGO87_000162"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0391595.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELGSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(24185..25006)
                     /gene="dapD"
                     /locus_tag="WGO87_000163"
     CDS             complement(24185..25006)
                     /gene="dapD"
                     /locus_tag="WGO87_000163"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0391596.1"
                     /translation="MSLQNIIETAFENRADITPSTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(25016..25246)
                     /locus_tag="WGO87_000164"
     CDS             complement(25016..25246)
                     /locus_tag="WGO87_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391597.1"
                     /translation="MPSERRRRGIKVLPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(25216..26859)
                     /gene="pgi"
                     /locus_tag="WGO87_000165"
     CDS             complement(25216..26859)
                     /gene="pgi"
                     /locus_tag="WGO87_000165"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0391598.1"
                     /translation="MNAFTRAWHALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEEGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGTIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            27369..28601
                     /gene="pilG"
                     /locus_tag="WGO87_000166"
     CDS             27369..28601
                     /gene="pilG"
                     /locus_tag="WGO87_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0391599.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            28675..29535
                     /locus_tag="WGO87_000167"
     CDS             28675..29535
                     /locus_tag="WGO87_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0391600.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLIAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLL
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGETGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            29537..30169
                     /locus_tag="WGO87_000168"
     CDS             29537..30169
                     /locus_tag="WGO87_000168"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0391601.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSQKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            30162..30371
                     /locus_tag="WGO87_000169"
     CDS             30162..30371
                     /locus_tag="WGO87_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0391602.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(30456..32132)
                     /gene="pilB"
                     /locus_tag="WGO87_000170"
     CDS             complement(30456..32132)
                     /gene="pilB"
                     /locus_tag="WGO87_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0391603.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         7535 a         9080 c         8218 g         7344 t
ORIGIN      
        1 attatggtag atactaactc tttaagttga catattgtgt gatatcgagg gctgtaccgt
       61 attttcccat cgtattttcc catcgtattt tcccatcgta ttttcccgtt ctttatatga
      121 gcggcaggca tcctcataaa agaagggata ccgataagga aaaatacccc agcataaaaa
      181 atatactgat taatcaatac aaatccaaca aacagttcat cgtattcttt tctgttggcg
      241 tataaaaata ctgatatatt ccttcacttt catagggcga cgctttatgt accgtcctgt
      301 gtgttgaaac gtcggttttc cttaccgcct gccccgcttt tccctgattg ctgccaactg
      361 ccgtccgaac ttgaaccgtc aggtttcaga cggcattttt tctgcctgct cccacaattg
      421 ccgtttttgt gcggcggtca gttttttgac ggcgatttcc tgcctgagtg ccgttccttt
      481 atccatcccg cctgcaacga tacgcatcgc caccggtttg aatacgcggg tatatttcgc
      541 gcctttaccg gttgtgtggg cggcaagccg ctgttgcgga ttcgggctga tgccgcaata
      601 gaacgcgctg ttttcacaca atatcaggta aacgctccag tttgacgcgt tcatctgcct
      661 gcatcctgag ttttctcccc gttccaaggc gcaactttca gcagcagcag catacccggt
      721 agggcgagtg cgaaacacag ctggaaaaac ggtacatacc cgagccattc gatcagataa
      781 ccggcaaagg aattgatgac cgtacgggga acagcggaca ggctggtaaa cagcgcaagc
      841 tgcgttgcgg taaatgcggg attggtttca cgcgccatat acgatacgaa cgccgccgtc
      901 cccaaaccca cgccgactgc ctctgcgccg ataactgccg ccagcatcag cctctcgcct
      961 gtgccgaccg tgtcgaaatg tccgaaccct gccagccata caaaccccaa aacggttaca
     1021 gcctgcaccg cgccaaatat ccacaaggct ttgtttacgc cgattttcag catccacacg
     1081 ccgcccaaga tacctgccgc cactgccggc cacagtcctg catttttcgc aatcaaaccg
     1141 atgtctgtct tgctgaaacc catatccaga taaaacggcg ttgccaacgc ggttgccata
     1201 ctgtcgccga gtttgtaaag gaagataaac agcagcacgc acaccgccga agcgatgccc
     1261 ttgcgcgtaa aaaattcttt aaacggctct accacggtct gcttcaacgt tttaggaacg
     1321 gcaggcggca acacgggttc gcgcgcaaga aacagcgtca tcagaaggcc gggcagcata
     1381 aacagcgaag tgatgacaaa cacctccggc cacggcatcc tgtctgccaa caccaaactc
     1441 aatgaaccgg gaatcagggc ggcaatccgg taggcgttca catgaaccga gttgcccaaa
     1501 cccaattctt cgtctgacaa aatctcgcgc ctgaacgcat ccaatacaat atcctgactg
     1561 gcggaaaaaa aagcgacaag caccgacaag ccggcaatca gcggcagatg attacggggg
     1621 tttaaaaagg cataggccgc caaagccgcc agcaaccctg cctgcgtcag cagcatccac
     1681 ccgcgccgcc gtcccaaaac gggcagcctg accgcgtcca tcagcggcga ccacaaaaat
     1741 ttccaagtaa acggcagacc gattaacgcc atcagcccga tgctcttcaa atccacctgc
     1801 tcgctgcgca accacgccgg aatcaggtta atcagaaagt acagcggcag ccccgaggta
     1861 aaccccgtaa aaatacagac aagcatattt cgggaaaaga tttgcccgat aaaacctgat
     1921 ttcgatgcag tcatcgttcg tgcagcccaa aaaaaacaaa agcctattgt agcaaatatc
     1981 gcccgcctgc tgaaaaatgc cgccccgcgc cgccggtttg ccgttcaaat gcaaaatgcc
     2041 gtctgaaact gtgttcagac ggcattgctt ttctgaaaac cagacgtgct tacaggctgt
     2101 aatacatttc aaattccagc gggtgcggcg ccatgcggat gcggcgtacg tcttcttctt
     2161 tgaaagcgat gtagctgtcg atccagtctt tgctgaacac gccgccgcgc aggaggaatt
     2221 cgtggtcggc tttgagggcg gccagtgctt cttccaaaga agcgcaaacg gtcggcacca
     2281 atgcatcttc ttccggcggc agatcgtaca ggtttttatc ggcagggtcg cccggatgga
     2341 ttttgttttg aatgccgtcc aaacccgcca tcaacagggc ggcaaatgcc aaatacgggt
     2401 tggcggtcgg atcggggaaa cgcgcttcga tgcggcgcgc cttgctgctg ttcacggacg
     2461 gaatgcggat ggaagcggaa cggtttttgg cggagtatgc cagtttggtc ggcgcttcaa
     2521 agtgcggcac gaggcgtttg taggagttgg tggacggatt ggtaatcgcg ttcaaggctt
     2581 tggcgtgttt gatgatgccg ccgatgtagt agagcgcggt gtcgctcaag ccggcatagc
     2641 cgtcgcctgc gaacaggttt tgaccgtctt tccaaatgga ttggtgaacg tgcataccgc
     2701 tgccgttgtc gcccataatc ggtttgggca taaaggtggc tgttttgccg aagttgtggg
     2761 caacgttttg aatcacatat ttcatgtctt gggtttggtc ggcgcgtttg accaaggtgg
     2821 cgaaacgcgt gccgatttcc atttggctgc cggtaccgac ttcactgtgg tggacttcga
     2881 cttcgatgcc gagttcttcc aaaatgttta ccatcgcgga gcgcaggtct tgtcccgcgt
     2941 caatcggagc aacgggcgcg taaccgcctt tgacggcagg gcggtggccg gtgttttgac
     3001 cgtccatatg cagaccgctt gcccacgcgc cgctttcgga cgtgatttcg taacgggttt
     3061 tgtgcatatc ggtttcaaat tctatgccgt cgaatacgaa aaactcgggt tcgggaccga
     3121 aataggcggt gtcgccgatg ccggaagatt tcaaataggc ttcggctcgg cgggcgatgg
     3181 agcgcgggtc gcggtcgtaa ccctgaccgt cggcgggatc gataacgtcg caagtcaaca
     3241 caacagtcgc atcatcataa aaaggatcga cgaaggctgt agacgcatcg gggcgcaact
     3301 gcatatcgga agcctgaatg cctttccagc cgccgataga cgaaccgtca aacgcctgac
     3361 cgttttcaaa ccactcttcg gggtcttcca acacgatgcg cgcaggcacg gtaaagtggt
     3421 gctgcttgcc tttggtatcg gtaaagcgca aatcgacaaa gcgggcttcg ctttcttcaa
     3481 tcaattttac ggcgttttta atagacattt ttcagctcct ggaaaaggga aatagcaaaa
     3541 tatgcggaac gcggcggctt gacgcaaagt cggcatgatt ctaccacgtc catgccgtct
     3601 gaaactcgcc ctgcgtcaaa tttcaagctg gaaaatatag tttcagacgg caagattgtc
     3661 aacaatatgc ttctgtttgt ctgttcaaaa gcggaatacc cgcccttttg tccgaatgcc
     3721 gacaaaacag atgccgtctg aaagcagttc agacggcatc tgttgtttgg tttgaaaacg
     3781 gtcagcgagg atagccgttg cagacaaatt tgtactgagc ctctccaagc gtaccgggaa
     3841 taacataatt ccattcggca taggatggtg tataagaagc gatagagttt cccgatttgg
     3901 aataccacgt tgccgtcaat atcctaaaac gtttaccact gcaatccatt tcttccaacg
     3961 ttttagctgt attaaaaaat tttccattgc catacttttg cggcttggcg aaccttgttt
     4021 caaaccatgc tctgttgtcc gtacggctgt ctgcatcacc aaacgtggta ttattgtcac
     4081 tgtcaatacc tacctgcacc caatccgccg ccaccgccgg tgttgccaac cccaatgcaa
     4141 atactgccaa gcacaacatc tttttcatgt ccgaccctct cgtccttgtc aacaaaataa
     4201 agttaaattg taaaaaaaaa acgacttttt tcaagagaaa aatattccgt catgctatct
     4261 gacaaccgtt tcagacggca tcgtttccat ctgtacgccg ccgttataaa atagccttcc
     4321 cgtcttttgg aacacgacca tgaccgccct cccccgttac tccgtttttg gcaaccccgt
     4381 tgcacacagt aaatcgccgc aaatccatca gcaatttgcc cttcaggaag gcgttgacat
     4441 tgaatacgaa cgcatttgcg ccgacatcgg cggttttgca caggcggttt cgacattttt
     4501 tgaaacaggc ggttgcgggg caaacgttac cgtaccgttc aaacaggaag cgtttcatct
     4561 ggcggacgag cattccgaac gcgcattggc ggcaggtgcg gtcaatacgc tgattccgtt
     4621 gaaaaacggc aagctgcgtg gcgacaacac cgacggtatc ggtttgacca acgacatcac
     4681 gcaggtcaaa aatattgcca tcgagggcaa aaccattttg cttttgggcg caggcggcgc
     4741 ggtgcgcggc gtgattccgg ttttgaaaga acaccgccct gcccgtatcg tcattgccaa
     4801 ccgtacccgc gccaaagccg aggaattggc gcagcttttc ggcattgaag ccgtcccgat
     4861 ggcggatgtg aacggcggtt ttgatatcat catcaacggc acgtccggcg gtctaaacgg
     4921 tcagattccc gatattccgc ccgatatttt tcaaaactgc gcgcttgcct acgatatggt
     4981 gtacggctgc gcggcaaaac cgtttttaga ttttgcacga caatcgggtg cgaaaaaaac
     5041 tgccgacgga ctgggtatgc tagtcggtca agcggcggct tcctacgccc tctggcgcgg
     5101 atttacgccc gatatccgcc ccgttatcga atacatgaaa gccatgtaat atgttccgca
     5161 tcgtcaaatg gctgattgcc ctgcccgtcg gcatctttat ctttttcaat gcctatgtgt
     5221 acggcaacat cattacctac cgcgccgtcg cgccccatcg gactgccttt atgtcgatgc
     5281 ggatgaagca gtttgaacag gaaggtcgcg atgtcgcact ggattaccgc tgggtgccct
     5341 acaaccgcat ctcggtcaac ctgaaaaaag ccctgattgc ttccgaagat gcccattttg
     5401 ccgggcacgg cggcttcgat tggggcggca ttcaaaacgc catcaggcgc aaccggaaca
     5461 gcggcaaagt gaaggcgggc ggctcgacca tcagccagca gcttgccaaa aacctgtttt
     5521 taaacgaaag ccgcagctat atccgcaaag gcgaagaagc ggcgattacc gcgatgatgg
     5581 aagccgttac cgacaaagac aggatttttg aactgtattt aaactcaatc gaatggcact
     5641 acggcgtttt cggcgcggaa gccgcgtccc ggtattttta tcaaataccc gccgccaagc
     5701 tgaccaaaca gcaggcggcg aaactgactg cccgcgtccc cgccccgctc tactacgccg
     5761 accatccgaa aagcaaacgg ctccgcaaca aaaccaatat cgtgctcaga cgcatgggtt
     5821 cggcagagtt gcctgaaagc gacacggact gagtgccgcc gcaatataaa atgccgtctg
     5881 aaatatcatt cagacggcat atgctttctg cctttaccct tttctctaaa acgcaacggg
     5941 gcggtcatta tcgcttttgt ccttctacat aacggtcaga cacaaaacgt gtccgacacg
     6001 gacaacttcc caaaaacggc aggcaaaaaa aataaccgct tataagcggt tttttggtgg
     6061 ccagaggcgg aatcgaaccg ccgacacacg gattttcaat ccgttgctct accaactgag
     6121 ctatctggcc tttgtctgtc tcgttaagag atgcgtatta aaccaaatta cgggacgaca
     6181 tgcaagcata tcgggcaata ttctttgaaa acaggtttaa gtcaatgttt taaatttgaa
     6241 ttaatttatg taaaaacaac ataaatccgt atttttgccg cctgcccgac gatattaggt
     6301 cgcccgtctg aaaaatattt tcaatatttg aagttcttac ccagataaac agaacgaacc
     6361 tgttcgtttc cgaccaaatc atcaggtttt cccgatgcca acaccgtgcc gtctgaaata
     6421 atgtaggccc gatcgcagat gctgagggtt tcgcgtacgt tgtggtcggt aattaatacg
     6481 ccgataccgc gcgacttgag aaatccgatg attttttgga tgtcgatgac ggcgatggga
     6541 tcgacgccgg caaaaggctc gtccaacaaa ataaaatgcg gtttcatggc aagtacgcgg
     6601 gcgatttcga cgcgtcggcg ttcgccgccc gacaaagatg gggcaggatt attgcgcaaa
     6661 cgctcgatat tcaggtcggc aagcagtttt tcaacttcgc cgtcgatgcg gcttttgtct
     6721 ttggtactga tttctaaaat ggcgcggatg ttttgttcga cggtcatttt acggaaaata
     6781 gaggcttcct gcggtaggta gccgacaccc aggcgggcgc gttcgtgtat gggcaggtga
     6841 cgcaattcct gcccgtccag catcacgctg cccgcatcgg cggcaatgag tccaacaatc
     6901 atgtagaagc tggtggtttt acccgcaccg ttgggcccga gcaggccgac gacttccccg
     6961 ctttcgattt caagggagaa gcttttaacg acttggcgtt ttttgaaact tttttgcagg
     7021 ttttgaacaa caaggcggct gacgtttgcg ctcataaata tctcttcaat atcttcggtc
     7081 ggcaaatgcc gtccgaacca ggtttatgtt tcagacggca ttttgggatt attcggattt
     7141 ttgcgtactc gaaggctgga taacgacgct gaccctgccg gatttggaag cggattttgc
     7201 gccggatttt gtgctgccgc tgatggtata gacttcggtt ttggtgttgt atgtaatcac
     7261 cgcaccttcg gcgacatcgc cgccgcgctg tactttggca ttaccggtta agactacggt
     7321 gctgcctgca gatgaataag caacgttgtt tgcctgtccg cgcaccgtgc ctttgccgcc
     7381 gtccaatgtc tggctgaagc ggactggcga accttccgcc ctcacggatt cgccgccttt
     7441 gccgccgcgt gtaacattga cgcgggcggc ggaaatattg agcgtaccct gtctgatgac
     7501 gacgtttccg ctgaatgtgg tgctttggtt ggcttgatcg agcgaacctt ggtcggcctc
     7561 aatctgaata ggctgcctgc tgtcgctttg aagggcaaaa gcgggggacg ccgaaaaaaa
     7621 tgctattaaa acaaatagct tacatatctt ttgtatcata aatcgtggct ttcactttag
     7681 atgagaagtt caacatgcct gttttgtggt cgtaagtcat gccgcccgcc tgaccgtgcg
     7741 atgcaccata ttggaaactg acaggcgtat cggtttgggc atattgagat tcggtatcga
     7801 cgtgcagctt ttcggcttca actttacccg cctgccgttt gccgtcggcg gttttggtca
     7861 gcacaacgtt gtttttaaaa agaacctgtt tgttttcggt atggtaaacg gcttcatcgc
     7921 tgccgacttc gtacaacaac ctgccttctt ggaagaacac gagatgcggt aaatcgaaat
     7981 gaatgtcgct gctttcggga aactgtttcg cgcctttcgc gctcaaatgt tctttcaagt
     8041 atccctgttc gtcaaaccgc ctgccgtcca agccgtccat tgtgtattgg ggttcgttgg
     8101 gattgagcct gacttcctcg atttcgactt cgctgatacg acccaaccat gccgacagac
     8161 cgcccaaggc aaccgccaat atcaatggga acgcaattcc gtaccgccat cttactttca
     8221 tttgatgtac tcgttcaaag ccgcgcccag cgtacctttc gcgcgcataa tcaggtcgca
     8281 cacttcgcgc accgcgcccg caccgccggg gcgtttggca atataggcag cgtgttgcag
     8341 cgtaaaccaa tgcgcgtcga ggacggcaac cgacaatccg caacgcacca tcaccggcaa
     8401 atcgaccacg tcgtcgccga caaaggcaca ctcggcttct tccacgccga cctgagcgcg
     8461 cagctcttca tacgcggcgc gtttgtccga aatgcctttg aagtagtaat ttatgcccaa
     8521 ctgtttgacg cggatgccga cggagggcgc gtcccgacct gtgataatcg cagtctgcac
     8581 gccgcttgcc tgaagcattt tcagaccgtg tccgtccagt gtgtgaaacg atttgatttc
     8641 ttcgccgtta tcgcggataa agatgcgccc gtcggtcaaa acgccgtcca catccaggat
     8701 caacagtttg attttggcgg cgcgcgcctg taattcggga gaaattgcct gcatgggatt
     8761 tccttgtttt cagacggcat atcttaccac aaatgccgtc tgaaacggct gcaacgcctt
     8821 atacaatccg tgccgccagc aggtcgtgca tattcagcgc gccgatcagc acgccatctg
     8881 catcggtaac cagaagcccg ttcacatggt ttgcctgcat gactttcagg gcttcggtgg
     8941 cgagacgttc ggcggagatg gttttaggat gcgtatgcat gacttcgtct atcgaaagac
     9001 cggtaaaatt gtcgcattct tgaaacaggc ggcgcaaatc gccgtcggtg aatacgcctt
     9061 tcagacggcc ttgcccgtcc gttaccgcca acatgcccag ccctttttca ctcatgctga
     9121 cgatggcttc tttcaagggc gtgccgagtc ggacggcagg caggccgccg cctttgtgca
     9181 taatgtcggc aacgcgcaaa agtaggcgtt tgccgaggct gccggcagga tggctcaagg
     9241 cgaaatcgtc gggcgtgaac gcgcgtgcgc gcagcaggac gaccgccaac gcatcgccca
     9301 aagccatgac ggcggtggtg ctggtggtcg gggcaagccc cagcgggcag gcttctttgg
     9361 aaaccgacgc cgtgatgtgg atgtcggcat ggcgcgccat ggttgaatcg gggcgggcgg
     9421 tgatgcagac aagcgtgatg tctttgcgtt tgagcgcggg gatgatggcg gcgatttcgt
     9481 cgctttcgcc ggaattggaa atcgcgacga ccacgtcgtt gtccacaatc atgcccaaat
     9541 cgccgtgtgc cgcttccgca gggtggacga aaaacgcggg cgtgccggtc gaggccatgg
     9601 ttgccgccat tttgcgcccg atatgtcccg acttgcccat gcccgtgata acaaccctgc
     9661 ccttgcagtg caacaacgcg tctgccgcaa ggacgaagtt ttcgtccaat tccgctgcaa
     9721 tttcgcgcaa gccttccgct tcggtgtgca acacttcgcg cgcccagtcg agatattttc
     9781 cgttttctgc cattgccatc cgcctttgct gtaacaaaac tacccaaaat ttccgaatcg
     9841 gggtagcata agcattcaaa ccccgccatc catccaaagg aatcagaaat gactatttta
     9901 tcggacgtta aagcattagg acaacaaatc tggctggaca acctttcccg ctcgctcgtg
     9961 caaagcggcg aattggcgca aatgctgaaa caaggcgtgt gcggcgtaac ttccaatccc
    10021 gccatcttcc aaaaagcctt cgccggcgac gcgctttacg ccgatgaaat cgccgccctc
    10081 aagcagcaaa acctcagccc caaacaacgc tacgaaacca tggcggttgc cgatgtgcgt
    10141 gcctcctgcg gcgtttgcct tgccgaacac gaatccaccg gcggcaaaac cggtttcgtc
    10201 agcctcgaag tttcgcccga attgtccaaa gacgcgcaag gcacggtaga agaagcgcgc
    10261 cgcctctacg ccgccatcgg gtgcaaaaac gcgatgatta aagtgcctgc caccgacgca
    10321 ggcatcgatg cgctcgaaac cctcgtttcg gacggcatca gcgtgaacct cacgctgctg
    10381 ttctcacgcg cccaaaccct caaagcctac gccgcctacg cgcgcggcat tgccaaacgc
    10441 ttggcagctg gacaaagcgt tgcccatatc cacgtcgtcg ccagcttctt catctcccgc
    10501 gtggacggcg cgctggacac aacactgccc gaccacctca aaggcaaaat cgccatcgcc
    10561 cttgccaaag ccgcctacca agactgggct caatacttcg gcagcccgga atttgccgcg
    10621 ctggaagccc aagtcgcaaa ccgcgtgcag cttttatggg catccaccgg cgtgaaaaac
    10681 ccggcctatc ccgacacact ctacgttgac agcctgatcg gcgcgcacac cgtcaacacc
    10741 gttccagatg ccacgctcaa agcctttatc gaccacggca cagccaaagc cacgctgacc
    10801 gaaggcgtag aagaagcaca agcgcagctt gccgaaaccg ccgcgctcgg catcgatgtc
    10861 gaaaccttgg cggcacgttt gcaggaagac ggtttgaaac agtttgaaga agcgtttgaa
    10921 aaactgctcg caccgttggt ttaaactttt catccccgat gccgtctgaa aggctttcag
    10981 acggcatcgc ccgtttgacc ggacacagtg cggcatatca aggattcacc ctaaaaatat
    11041 taatgggcaa gaagttaatg cacgacatta cccaccaaca caaaaggaaa tagaatcatg
    11101 gcaaaagcac tcgaaatcat ttcacccaat gaaatttatt cagatctgat ttttaaggat
    11161 ccggtacctc cccatacaat atatacagaa ttgatgaaac tggttagtag aaatgcaagt
    11221 aatgagagaa tcgcagatag agccttcgat tttttctcgt ccggccttat ggatggttca
    11281 gctaccgaag agcaatataa tgccctatat gatttaacct tacttgaaga acccgggatg
    11341 gaactgaata aagatgagat aatggctttg attaatagtc ttaaataaag gggccgtacc
    11401 ggatatgtac acaggacgtt tcgcccccag cccgaccggg ctgctccaca tcggctcgct
    11461 gctgaccgcc gccgcttcct atgccgatgc acgctcaaac ggcggcaaat ggctggtccg
    11521 catggaagac ctcgatccgc cgcgcgaaat gccgggggcg gcaagccata tcctgcacac
    11581 gcttgaggca ttcggattcg agtgggacgg agaagtcgcc tatcagagcc gccgttacgc
    11641 cctgtatgaa gaaacactat gccgcctgaa aaccgccgga ctggtctatc cctgccattg
    11701 cagccgcaaa gactggcaga ccggggcaag gcggggcgca gacgggttcg tctataacgg
    11761 acgctgccgc aatccgcagc aacgccctgc accgcaaggc aaacagcctg cgtggcgcat
    11821 ccgcgtcccc gaccgcgaca tcggcttttc agacggcatc gtcggcggtt acgcccaaaa
    11881 cctcgcccgc gacatcggcg atttcgtcct gcttcgtgca gacggttact gggcatacca
    11941 gctcgccgtc gttgccgacg atgccgaaca gggcgttacc cacatcgtcc gcggacaaga
    12001 cctgctcgtt tccacgccgc gccaaatcta tttgcagcag tgtttgggcg ttccgacacc
    12061 gcaatatgcc cacctgccgc tgctgaccaa cgcacaaggg cagaagtggt cgaaacagac
    12121 gctcgccccc gcattggatt taaaccgccg cgaacaactc ctccgccaag tgttccgtta
    12181 cctcaagctg cccgaagcac cggaaaccga ccgccctgcc gaactgctcg actgggcggt
    12241 ggcacactgg gatatgggca aagtgccgaa acacgccatt accaccccct aaacaccgcc
    12301 cgacaaacgc acaaatgccg tctgaagcct tcagacggca tttcacacat acagccccgc
    12361 cctattcgcg cgtatttgcc cgctcaacct ctttccacgc ttcgagaatc aggctgccgt
    12421 cgttgtcttt caagagcgtt gcgtcggaaa gcatacgccg ccaagtccgc gcacctttca
    12481 aaccgtgcat cagcccgagg ctgtggcgga cgatgtggcg caagattgtg ccgcgtccgg
    12541 cttggatctg ggcttggctg tatgtgtaga ggcactgcac caaatcggcg tattcgatcg
    12601 ggctgcgagt atcgccgtaa aacaatctgt cccattcgcg catcaccatc gggttgtggt
    12661 acgcctcgcg cccgaccatc acgccgtcaa cgtgttgcag gtgtccggcg attgcttcgt
    12721 tggtggtgat gccgccgttg atgatgattt ccagcccggg aaactcctgc ttgaggcggt
    12781 aaacgtaatc gtatttcaac gggggaacgt cgcggttttc tttgggggaa agaccgtcca
    12841 gccaagcgtt gcgggcgtgg acgatgaagg ttttgcaggc ggttttgtcg cgcagcgtgc
    12901 cgacgaaatc ggcaacggtt tggtattcgg tctgcctgtc cacaccgata cggtgtttga
    12961 cggtaacggg aataccgacc gcgtcctgca tggcgttgag gcagtcggca accagcccga
    13021 cttcgttcat cagacacgcg ccgaacgagc ctttctgcac gcgcggactg gggcagccgc
    13081 agttgaggtt gacctcgttg taaccgtatg cctcggcggc tttggcggct ttcgccaaat
    13141 cggacggatc gctgccgccc agttgcaggg cgacgggctg ctcgccttcg ttgaacatca
    13201 aaaagcggtc tttgtcgccg taaacaatcg cgccggcgtt gaccatttcg ctgtacagcc
    13261 aagtattgcg ggtaatctgg cgcgccatat aacggtagtg cctgtccgtc cagtccaaca
    13321 tcggggcgac ggacaggtgg cgcgggggaa gggtgtgtgt attgtcgttc attgtcgata
    13381 ttttatcatt gcggattcaa tttcaatccg gaacaacctt catttttggg gaaatccgcg
    13441 tcatgaaaga gtccagcagt atggttaccg gcccgtcgtt gcagagcgac acctgcatat
    13501 gcgtgcggaa acgccctgtt tcgacatgaa ttccgtgtcc gcgcaacagt tccgccgttc
    13561 gcaggtaaag ctgctgcgcc tgttctgcag gtgcggcttg cgaaaacgaa ggccgccgcc
    13621 cgcttgtcgc gtcggcataa agcgtaaact gcgacaccag cagcaccgcg ccgccgacat
    13681 ctttcaaaga caggttcagc ttgcccgctt cgtcttcaaa cacgcgcaaa tgggcgattt
    13741 tgtcggcgat atagcgtgca tctttttctg tgtcgctatg cgttacgccg agtaacacga
    13801 caaacccgcc gtcgattttg ccacaggttt ccgtgccggc ttcggacacg acatccacct
    13861 ttgcacctac cgttttctgt atgaccgcac gcataagccg tcctccttac ctgaacgtgc
    13921 aatgccgtct gaaaggcttc agacggcatc ggtattggaa atgccattac ggtttgacac
    13981 cgttttcttc caaaagggca tcgattttca agcgggcttt ttcctgtctc aaaccctgct
    14041 ccaactggtt tctgaccaac tcgaaaggct gcgcgtcggg gtttttcccg acctcgctga
    14101 gtttgaacag ataatagcgt tcgcccaatt tgaccggatc gcgggtaacg tcgccccgat
    14161 tcatcgcggc aaactgcgaa gccagcggct cgggaagctg ctgcgccata atgaaaccgt
    14221 caaaagcctg ctcgtcgttc ggataacgct tcatcagccc ttcaaaagac agccctttga
    14281 gcaggagctg ctgcgcctga cgcgcctcct cttcggttgc gaagctgacc tgctgcaatt
    14341 tgatcatgcg gatttgctgt tcgtaaaact tgtgcagctc gtcttcggaa accgtttccg
    14401 aacgttccag aaaacggacg tactcctcgg cataaaaaga cgcttcggcg attttaaagc
    14461 ggttttggac atccttatcc ttatccaaac cttccttcaa tgccctgttt ttcaaaactt
    14521 ccaaagtttg tagccggcgg acggcatcgt ttcggattgc ctgcccgtcc ggtttttggg
    14581 actgctccgc atgccggtct gcctgctgca tgatctgcgc caccagcgta tccaccaaag
    14641 ccgggtcgat ttcgggtgct ttggctgccg caaaacctgc caacattgca gcaataactg
    14701 cggcagcggt ttttttctgt ttcatggtat tccctgtcct gcgcggcggc aaaacgcctg
    14761 tctgaacgtc ttgccgccat atcccgtttt cagaattatt ttgcaggttt gatgtttgcc
    14821 ttgcccaaca gtgcaccgac ggcacggtca atccgttccg cctgaaggtt gcccgcaatc
    14881 tgtcctttca tttcatcaaa agaaggcact tttacctcgc ggctgtcgtt gacataataa
    14941 acgccgtaga aatcgccgtt tttcagcggc gttgccgtaa attcgccttt tttcaagtcc
    15001 ttaattgcct gataaagcgg cggaacaccc tgttccaaat ctttcagcgg cacatatccg
    15061 accggcgcac cggtctgttt ggtacggtcg ttgagggaat attgtttcaa gacggcatcg
    15121 aaaccttttt tcgccttcaa gtcggcaacc gctttttttg cattttcttc cttgtcggtc
    15181 aggatttcgc ccaactggac ttcctgcgta cctttgtaaa aaccgctgat attgtcatat
    15241 gcggctttta cttcctgctc ggaaaccggt tgggttttgg cgatatgcaa tgcgtatgcc
    15301 tcgccgttca agccatattt taccgcctgc caaacggttt tgaaggacgg tttcttgtcg
    15361 tcgcccgact ttttcgcttc ggcacgcaat ttggcaagcg catttttaaa ctctgccgac
    15421 cggtcgagtt tcaggcgttt cacttcctgt gcgaccacgg tgttgaccac ttcgttttcc
    15481 agcagggatt ggcgcagttg cggcgtgtct tcggcacggc tgttttccgc acggaatgcg
    15541 gcaacctgcg catcgatgac ggaactgtcg attttctgac cgttgacggt tgccagcgtt
    15601 tgggcaaaca ggctgccgga acaggcaagc agtgcaacgg aagtcaggat ttttgctttc
    15661 ataatcttct ctctttaaat tttaaattga aatgttcggg cagacttcat tcagtccgcc
    15721 gtatggaaat actcgtcagg ggtagccgcc cggatgccga gcgcgtgaat catgtcgcct
    15781 gaaaacaaat ctttgagcag cgatttgacc gttttctggc ggttcaggcg gcttacgcct
    15841 tcaaaacggc cgctgacgac caaaacggca taatgtccgc cgccggtatt gcccgcgtgt
    15901 cctttgtgca gatggctctc atcgccgatt tccaacacca gcggatcgag cgtctgcagg
    15961 cgttcgcgga tcaaatcgac ggccggcatt atttgaacac cgctttaaac ggcttgatca
    16021 gcacttcgct gtacacgcct gcatgaacat agggatcgtc ttccgcccac gcctctgccg
    16081 catccaaaga ctcgaactgc gccacaatca aactgcccga aacgcggttg gagtcctccg
    16141 gcaaaggatt cgggcctgcc gtcaacagcc ggccttccga cttcagcgtc tccagccgtt
    16201 tgaggtgttc gggacgtgcc gccatacgcg cttcatacac attctccccg tctgttgcca
    16261 gcaacataaa atattccaca gtcaatcctc ctttttcaga caggtactca gataaatacc
    16321 ctgaataata aagaaaacaa gcatcagtgc agtcgagccg aacattttat agttgaccca
    16381 ttgcgactcg aaccgggtaa acacaaacca gttggcgata cccataaaaa tcaggaaacc
    16441 gacccacata tacgtcaatt tcgcccatac ggcatccgga agctgaatct ccctgccgat
    16501 actcgccttc aagccgtttt taccggcgag gtggctgccc cacaggaaca gcgcgcccag
    16561 ccaaaacaaa acgctcggct tccacataat gaagcggctg tcgcccaaaa caatggttgc
    16621 gccaccgaat atcacaatca gcaccagtcc gacccactgc atcgtatcca gctttttata
    16681 tttccaatac agaaaagccg cctgaaccac accggcaacc aaggcgaccg ccgttgcggc
    16741 aatcatgttt ttggtaacgg tataggtggc gaaaaacagg atgacggaca aaaggtcgct
    16801 gacgaatttc atatccaaat gagaaagcaa atcaaataaa cgctaatcat acaggcagaa
    16861 ccctgttttt gcacctgcaa aaccaacaaa ttttgttttg attgatgcaa atatgcacag
    16921 atgtttttga aaaaagatgg agatatgtca taatttgtaa aaacggcgaa tgattgccgt
    16981 ttaaaatgtg gcggcggtcg gtacattcac atattaaatg cccgccggcc gactgccccg
    17041 ccgatgcccg atgatgacga aatttacaga ctgtacgcgg tcaaaccgta ttcagccgcc
    17101 aacccacagg ggatacatct tgaaaaacaa cagacaaatc aaactgattg ccgcctccgt
    17161 cgcagttgcc gcatcctttc aggcacatgc tggactgggc ggactgaata tccagtccaa
    17221 ccttgacgaa cccttttccg gcagcattac cgtaaccggc gaagaagcca aagccctgct
    17281 aggcggcggc agcgttaccg tttccgaaaa aggcctgacc gccaaagtcc acaagttggg
    17341 cgacaaagcc gtcattgccg tttcttccga acaggcagtc cgcgatcccg tcctggtgtt
    17401 ccgcatcggc gcaggcgcac aggtacgcga atacaccgcc atcctcgatc ctgtcggcta
    17461 ctcgcccaaa accaaatctg cactttcaga cggcaagaca caccgcaaaa ccgctccgac
    17521 agcagagtcc caagaaaatc aaaacgccaa agccctccgc aaaaccgata aaaaagacag
    17581 cgcgaacgca gccgtcaaac cggcatacaa cggcaaaacc cataccgtcc gcaaaggcga
    17641 aacggtcaaa cagattgccg ccgccatccg cccgaaacac ctgacgctcg aacaggttgc
    17701 cgatgcgctg ctgaaggcaa acccaaatgt ttccgcacac ggcagactgc gtgcgggcag
    17761 cgtgcttcac attccgaatc tgaacaggat caaagcggaa caacccaaac cgcaaacggc
    17821 gaaacccaaa gccgaaaccg catccatgcc gtccgaaccg tccaaacagg caacggtaga
    17881 gaaaccggtt gaaaaacctg aagcaaaagt tgccgcgccc gaagcaaaag cggaaaaacc
    17941 ggccgttcga cccgaacctg tacccgctgc aaatactgcc gcatcggaaa ccgctgccga
    18001 atccgccccc caagaagccg ccgcttctgc catcgacacg ccgaccgacg aaaccggtaa
    18061 cgccgtttcc gaacctgtcg aacaggtttc tgccgaagaa gaaaccgaaa gcggactgtt
    18121 tgacggtctg ttcggcggtt cgtacacctt gctgcttgcc ggcggaggcg cggcattgat
    18181 cgccctgctg ctgcttttgc gccttgccca atccaaacgc gcgcgccgta ccgaagaatc
    18241 cgtccctgag gaagagcctg accttgacga cgcggcagac gacggcatag aaatcacctt
    18301 tgccgaagtc gaaactccgg caacgcccga acccgctccg aaaaacgatg taaacgacac
    18361 acttgcctta gatggggaat ctgaagaaga gttatcggca aaacaaacgt tcgatgtcga
    18421 aaccgatacg ccttccaacc gcatcgactt ggatttcgac agcctggcag ccgcgcaaaa
    18481 cggcatttta tccggcgcac ttacgcagga tgaagaaacc caaaaacgcg cggatgccga
    18541 ttggaacgcc atcgaatcca cagacagcgt gtacgagccc gagaccttca acccgtacaa
    18601 ccctgtcgaa atcgtcatcg acacgcccga accggaatct gtcgcccaaa ctgccgaaaa
    18661 caaaccggaa accgtcgata ccgatttctc cgacaacctg ccctcaaaca accatatcgg
    18721 cacagaagaa acagcttccg caaaacctgc ctcaccctcc ggactggcag gcttcctgaa
    18781 ggcttcctcg cccgaaacca tcttggaaaa aacagttgcc gaagtccaaa caccggaaga
    18841 gttgcacgat ttcctgaaag tgtacgaaac cgatgccgtc gcggaaactg cgcctgaaac
    18901 gcccgatttc aacgccgccg cagacgattt gtccgcattg cttcaacctg ccgaagcacc
    18961 gtccgttgag gaaaatgtaa cggaaaccgt tgccgaaaca cccgatttca acgccaccgc
    19021 agacgatttg tccgcattac ttcaaccttc tgaagtacct gccgttgagg aaaatgcagc
    19081 ggaaatcgtt gccgatgatt tgtccgcact gttgcaacct gctgaagcac cggccgttga
    19141 ggaaaatgta acgaaaaccg ttgccgaaat acctgatttc aacgccaccg cagacgattt
    19201 gtccgcatta cttcaacctt ctgaagtacc tgccgttgag gaaaatgcag cggaaaccgt
    19261 tgccgatgat ttgtccgcac tgttgcaacc tgctgaagca ccggccgttg aggaaaatgc
    19321 agcggaaatc actttggaaa cgcctgattc caacacctct gaagcagacg ctttgcccga
    19381 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
    19441 tatcccaaat aatgcagata ccagtttccc ttcggaatct gtaggttctg acgcgccttc
    19501 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
    19561 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
    19621 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
    19681 ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa ccggcacagg
    19741 ctcaaacagc aggccgacgg caacaaaatg ccgtctgaaa cccctaaagg cttcagacgg
    19801 cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcacc gcttttcttt
    19861 tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac ggttgtgccg
    19921 tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac acgttcgcag
    19981 gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc caagtcgtat
    20041 cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc gtcgccgtaa
    20101 ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag cagtttcata
    20161 cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg gagcatagta
    20221 tggagtaagc gcatttttgt gttcctctcg gtagtggtta aacttcgatt ttgtttggcg
    20281 taaacgtctg ccatttgttg caggcaggac aatgccagaa aaagacttgt gatttgaagt
    20341 ggcagtttcg gcaccggtac atcacgctgc gctgtagctg ccgtccgata accgaacgca
    20401 tcatatcggc atcggctttc caagccggat ccaaatcgct gagtttcaaa ccaagcaggc
    20461 ggtacacgcc gttgaggtcg ggcttgcggc ggacaagctc gacggcggtt tgcgcggctt
    20521 ctttctcgca cttaagcagc agggatttct cgtacacgac attgatcagg tcaagttcgg
    20581 gaaacgtctg catatatcct gtcagacggt tcaagccttc ttcaggtttt ccctgcgcgg
    20641 cataggcttc gtaaagcttc tcgccgacca tgctcaagta tgcatggttt tgctgctcga
    20701 tggcggcata ggcttcgacg gcggcaggga aattgccttg tcggtgttcg atgtcgccca
    20761 aaatcatgtt ggcacgggtg cattttttgt tggcttcgag tgccttgccg acattgaaac
    20821 gcgcgacatc gaaattggac ttgaacagcg cggcttgggc aagttcgcaa taaaactggg
    20881 cgatttcaaa ctgataggtc tgatcgtcat ggctgagcag ccgggcggtt tcaaccgctt
    20941 tttcccaatc cctgtcctgt tggtagatat tgagcaggtg ctgtctggct tcacgcgcca
    21001 ttttaccgtc ttgcagcccc aaaaaaatct gttcggcacg atcgaccaac cccgcacttt
    21061 ggtagttttg cgccaattca aacaggacgc gcgcgcgctt ttcgccgacc gtatcgggag
    21121 aatcgagcat tgtccggtgt atgttgatgg ctttgtcgtt ttcgccacgc tgacggtaaa
    21181 gcttgcctag ggtgaggttt aggtcgtatg actgaggctg ctgatcaatg acctctgcca
    21241 attccctggc ggcacgtccg ctgttgcggt caaccaaggc atccagactt ttataaaatc
    21301 ccgacggtat gctttttgcc tgctttaata cagtcttcat atccacacgg gcggcaaacc
    21361 agcccatcgc gaagaaaacg ggcaaaagga taatcggcag caggataatc cacaattcat
    21421 tgtccatatc ggctttctca aggctgtttg gcagattcgg gcgcattttg cgccggtggt
    21481 gcggtcagct ctttccccgt caaacgcgca tttttcttta cttcggcacg caacctgccg
    21541 ttctcgccac gtaacgacaa caaccgtccg aacaaggcaa acattccaaa aacaataccg
    21601 actacaaatg cgccgaacaa tacgacaatc agcggcaaat cgaatttttg ccccggcagg
    21661 taggaaaagg taacggcatc cgtattaatg acggcaagca gcaggaagag cagcaggata
    21721 atgattttga tgacggtata gataagtttc atgtttgctc cgtaaataaa aacgccgccc
    21781 ggacagcttc gcactctgaa ccaagattgc gctagtttaa acgatttgca cggtttcgga
    21841 taggatgcgg cagcgtgttc ggacgacata cggagtatgc gattgccgac aattttacca
    21901 aatacaccgt tcctttccat ttgaaaaata acggattgga caccgcatcg acagaaaaac
    21961 ccgccgcgca cttgtcaaaa ccctgtttgc aggcgtatct ttacaatctt caaattcaaa
    22021 ccgttcattg aaacatatca gaataagaaa ggctttacat catgagcaga cccgtacccg
    22081 ccgtattcgg cagcgttttt cacagtcaaa tgcccgtcct cgcctaccgc gaaggcaaat
    22141 ggcagccgac cgaatggcaa tcttcccaag acctctccct cgtaccgggt gcgcacgccc
    22201 tgcactacgg cagcgaatgt ttcgagggac tgaaagcctt ccgtcaggca gacggtaaaa
    22261 tcgtactgtt ccgtccgact gccaatatcg cgcgtatgcg gcaaagtgcg gacattttgc
    22321 acctgccccg ccccgaaacc caagcctatc tcgatgcttt ggtcgaactg gtcaaacgtg
    22381 ccgccgacga aattcccgat gcgcctgccg ccctgtacct gcgcccgacc ttaatcggta
    22441 ccgatcccgt catcggcaag gccggttctc cttccgaaac cgccctgctg tatattttgg
    22501 cttcccccgt cggcgactat ttcaaagtcg gctcgcccgt caaaatttta gtggaaaccg
    22561 aacacatccg ctgcgccccg catatgggcc gcgtcaaatg cggcggcaac tacgcttccg
    22621 ccatgcactg ggtgctgaag gcgaaagccg aatacggcgc aaaccaagtc ctcttctgcc
    22681 cgaacggcga cgtgcaggaa accggcgcgt ccaactttat cctgattaac ggcgatgaaa
    22741 tcattaccaa accgctgacc gacgaatttt tgcacggcgt aacccgcgat tccgtactga
    22801 cggttgccaa agatttgggc tataccgtca gcgaacgcaa tttcacggtt gacgaactca
    22861 aagctgcggt ggaaaacggt gcggaggcga ttctgaccgg tacggcagcc gtcatctcgc
    22921 ccgttacttc cttcgtcatc ggcggcaaag aaatcgaagt gaaaagccaa gaacgcggct
    22981 atgccatccg taaggcgatt accgacatcc agtatggttt ggcggaagac aaatacggct
    23041 ggctggttga agtgtgctga tgctttaaat aaaaaaatgc cgtctgaaac ccgtttggcg
    23101 tttcagacgg cattttcgcg ttcggaccat ttccgctgca cctgcagcaa gtcgggcatt
    23161 tccctagaaa tacgcttcag acagcaaacc gccgcccgaa accgatacgc ggtacggacg
    23221 gcgcacggat ttgaaaacgg cggattatcc ctcggtgctc aaggcattaa tgctgtaacc
    23281 gccgtcaacg taagtgattt cgccggtaat gccggacgac aggtcggaca gcaggaaggc
    23341 ggcggtattg ccgacttctt caatggtaac gttgcggcgc agcggattgt gggcggcgac
    23401 gtgtcccaaa agtttgccga aatcggcgat accggaggcg gcaagcgttt taatcgggcc
    23461 ggcggaaatg ccgttgcagc ggatgccttc tttgcccaga caggcggcgg taaaacggat
    23521 gcccgcctca aggctggctt ttgccatacc catcacgttg taatttggaa tcgcgcgcac
    23581 cgcgcccaag tagctcaggg caacgatggc ggagtttctg ccgcgcatca tcggacgggc
    23641 ggcttttgcc aatgcgggca ggctgtatgc ggaaatttcg tgtgcggtgt tgaacgcttc
    23701 gcggctgatg ctgtcgagga agtcgccgct caaggcttct ttcggcgcga agccgatgga
    23761 atgcaccaaa ccgtccaagc cgtcccaatg tttgcccaag tcggcgaaca cttggttgat
    23821 ttcgtcgtcg ctggcgacat cgcagcggaa tacgagttcg gaacccaatt ccgccgccat
    23881 tttgcggacg cgctcttcca gtttgtccac aacgtaggta aacgccagtt ccgcgccttg
    23941 ttcgcggcag gctttggcga tgccataagc gatggaacgc tcggaaatca tgccggtaat
    24001 cagaattttt ttgccttgca gaaaacccat tttgtatcct tcaaatagtg ttcggttcat
    24061 tctaaaccgt tgattatagc aaattgtccc tgtttctgtg ttttcacgtt gcagcatgca
    24121 aacggcaatg ccgtccgaag cggatttcag acggcatcgg acgtttcaaa tacggtttaa
    24181 ggcatcagat gccgcgcaac aattcgttca cgctggtttt cgcacgggtt tgcgcgtcca
    24241 cgcgtttgac gatgacggcg cagtaaaggc tgtggctgcc gtctttggaa ggcatactgc
    24301 cggacacgac gaccgaaccg gccggtacgc gaccttggta gatttcgccg gtggtgcggt
    24361 cgaagatttt ggtggattga ccgatgaaca cgcccatcga aatcacgctg ccttcttcga
    24421 caatcacgcc ttcgacgatt tcggaacgcg caccgatgaa gcagttgtct tcaatgatgg
    24481 tgggtgcagc ctgcaggggt tcgagtacgc cgccgatgcc gacaccgccg ctcaagtgga
    24541 cgtttttgcc gatttgcgcg caagagccga cggtcgccca agtatcaacc atcgcgcctt
    24601 cgtcgacgta tgcgccgatg ttgacgtaag acggcatcag cacgacattt ttcgccacaa
    24661 agctgccgcg tcgggcaacc gcacccggaa cggcgcggaa gcctgcgttt ttgaactcgt
    24721 cttcagacca gtcggcaaac ttggtcggca ctttgtcgaa gtatttgttc acgccgtcgt
    24781 tgaggacttc gttgtcttgg atgcggaagg acagcaacac ggcttttttc gcccattcgt
    24841 tgactttcca ctcgcccacg cccaagcgtt cggcaacgcg cagtttgcct gaatcgagtt
    24901 ggcggatggt ttccaacacg gcttctttaa cttcgggagc aacagtggac ggggtgatgt
    24961 ccgcgcggtt ttcaaaggcg gtttcgataa tgttttgcaa agacataatg tttccttatg
    25021 tgagatgttt cgtgggacgc ttgccggtat tttcaagcag tatgttcatt tgaaatgaat
    25081 gcgctattgt gccacgcaaa cgcatattcg tcagcaatac gcgggcggtt gccgaacatt
    25141 ggcgtttcgg aatcgggcgg gcggcaggta ttgccttttc cgcgcccgaa tcaatacaga
    25201 aaggcggcag tacttttatg ccgcgccgcc gcctttcaga cggcattcgc ggtaaaacgc
    25261 catcagccct tcggtcgagg catcgtgtac ggacgtgccg ccttccagtt cgccgatgat
    25321 ggttttcgcc aactgtttgc cgtattccac accccactga tcgaaggggt tgacgttcca
    25381 tatcgtgcct tggacgaagg ttttgtgttc gtaagccgcc atcagcatac ccagattgta
    25441 gggcgtgagg cggtcgagca ggatgctgtt actggggcgg ttgccgggga actctttgtg
    25501 cggcgcgagg cgttcgcgtt ccgcttcggg caaatctgcc aattcggcgc gtgcttcgtc
    25561 caaggttttg cctttcatca gggcttcagc ctgtgcaaag gcgttggcaa cggtaaaacg
    25621 gctgcgtcct tcctccacgc cctgcgccgt catcgggacg ataaagtcgc agggaatcag
    25681 gcgcgtgcct tgatgaagca gttggaaata ggcgtgctgg cagttgaccc cttcaccgcc
    25741 gaacacgatg ccgcccgttt tgcacacggc gggactgccg tctgaagcgc ggcttttgcc
    25801 caaactctcc atatcgagct ggttcagcca cgccggcagc aggcgcaggt tgtggctgta
    25861 cggaacggcg gtctgcccgt ccgcgtgttg gaaattgtta taccacacgg caatcagtgc
    25921 catcagaacg ggaatgttgc ggcgcggcgg cgtgtggaaa aaatggctgt ccatcgcgtg
    25981 cgcccccgcc aacaactcgc ggaaacgcgc cccgccgacc gcaaccatca cgggcaaacc
    26041 gacgggcgac cagacggaat agcgtccgcc cacccagtcg tacatcgcaa acacgcgttc
    26101 cgccgcgata ccgaaagccg cagctgccgc agtgtcggca gacaccgcgc aaaaatggca
    26161 cgccgtttcg gattccgaga accccgcacc gcgataccac gccttgactg cctgtgcatt
    26221 gagcagggtt tccggtgttt tgaaggactt gctggcaacg caaaacactg tcgtttcggg
    26281 gttcagacgg cataaaaccg catccaggca ggcaggatcg gcgttggcgg caaaatggac
    26341 ggcgatatgc cgtctgaacg gctcaagtgc ctgcacgcac attgccggcc cgaggtcgga
    26401 tccgcctatg ccgatgtgga caaaatccgt aatccgtttt ccggttgtcc cctgatacga
    26461 accgtcgtcc aaactgtgtg caaacttcaa cgcacgattt aactcgcggc ggatttcggg
    26521 caacacgtcc ctgccgtccg cataaacggc acccgcaccg tcgggcaggc gcaaagccgt
    26581 atgcagcgcg gcacgcccct cactgttgtt gaccttcgca cccgtccgca aagcacgcat
    26641 tttcccttcc aaatccgccg catcggcaag attgcagagc agttgcagcg tatcttcgcc
    26701 caaacggttt ttgctgtaat cgaacaacat cccgtccaaa cgctcgtgca tacgctcaaa
    26761 acggtcgggt tcgcaggcaa agcggtcgcg caaaaggaca tgacgcgtat cctgataatg
    26821 gcgttcgagc gcatgccatg cacgggtaaa agcattcatc tgttttcctt gatttttcag
    26881 aaccggatta aaatgtagca gaatgtagtt taacaaacgg cagcggcttt ggcgaatctc
    26941 cggaacaccg cccgccgcgt ccaatagcac cttatttcca tatggcgaaa atatagtgaa
    27001 ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta caaatagtat ggaaccgatt
    27061 cacttggtgc ttcagcacct tagagaatcg ttctctcttg agctaaggcg agccaacgcc
    27121 gtaccggttt ttgttaattc actataccct ccggccggca tttgacccgc aacaatatcc
    27181 tgcaagattt attgtgtacg cataaatgcc ggacagccgc ctaaataagg tataatcgca
    27241 tccgattctg tcccgtttgc cgcccgcagg cgggatgtcg gcggttttca tcttaccggg
    27301 aacacgccgg tactgttttt taacgatgtt tcttacattt tattccaatt actttacggg
    27361 gctagaatat ggctaaaaac ggaggatttt ctttgttcgc aaagaaagaa aaacgcttta
    27421 tctttgaagg caggcattcc gcctccgaca aactggtcaa cggcgaagta tccgcgttta
    27481 ccgaagaaga ggcgcgcaaa aaactggcaa aacgcggcat ccgcccgttg cagattaccc
    27541 gtgtgaaaac aagctccaag cgcaaaatca cacaagaaga catcaccgtt ttcacccgcc
    27601 agctgtccac gatgattaaa gcgggcctgc cgctgatgca ggcatttgaa atcgtggcgc
    27661 gcggacacgg caacccgtct atgacggaaa tgctgatgga aatccgaggc gaagtggaac
    27721 agggcagctc gttgagccgc gcattctcaa accacccgaa atatttcgac cgcttctact
    27781 gcaacctggt tgcggcgggc gaaacgggcg gcgtattgga aagcctgctg gacaaattgg
    27841 caatttacaa agaaaaaacc caggccatcc gcaaaaaggt aaaaaccgcg ctgacctatc
    27901 cggtatccgt catcgccgtc gccatcggtt tggtattcgt gatgatgatt ttcgtactgc
    27961 ccgcctttaa agaagtttac gccaatatgg gcgcggagct tcccgcactg acccaaacag
    28021 tgatggatat gtccgacttt ttcgtctcat acggctggat ggtgctgatc gcactgggct
    28081 ttgccatata cggcttcctt aaattgaagg cgcgttcgat taaaatccaa cggcgtatgg
    28141 atgccatact gctgcgtatg ccgattttcg gagacattgt ccgcaaaggg acgattgccc
    28201 gctggggcag gacgacggcg acgctgattg cggcaggcgt gcctttggtc gatgtattgg
    28261 actccactgc cggcgcggcg ggcaatttaa tctatgaaga agccacccgg gaaatccgta
    28321 cgcgcgtcat ccaaggtttg tctatgactt cggggatgcg tgcgacggaa ctgttcccca
    28381 atatgatgtt gcagatgtct tccatcggcg aggaatcggg ttctttggac gatatgctca
    28441 acaaagccgc cgaattttac gaagacgagg tggacaatgc ggtcggcagg ctgtccgcta
    28501 tgatggagcc gatcattatc gtgattttag gcttggtcat cggcacgctt ctggtcgcca
    28561 tgtatctgcc gctgtttaat ttgggcaacg tggtcgcctg atttgccgca cagatccggc
    28621 gcggattcgc tctgcgccgg tttgtttttg ttttgaatac attgagaaca aaatatgtct
    28681 gatttgtctg tattgtcgcc gtttgccgtg cctttggcag cggtgttcgg gctgctggtc
    28741 ggaagtttct taaatgtcgt catttaccgc gtgccggtca tgatggaacg cggctggacg
    28801 gtatttgcca aagaatattt aaacctgccg ctgaccgaag aggaaagccg taccttcaac
    28861 ctgatgaagc cggattcctg ctgtcccaaa tgtcgcgtgc cgatacgcgc gtggcagaac
    28921 atcccgattg tcagctacct gctcctgcgc ggcaaatgcg cttcctgcca aaccaaaatc
    28981 agcatacgtt atcccttaat cgagctgctg accggcgtat tgttcgggct gatcgcctgg
    29041 caatacggct ggtcttggat tacgctgggc ggattggtac tgaccgcgtt tctgatttcc
    29101 ctgaccttta tcgatgcgga cacccaatac ctgcccgact cgatgacact gctcttgatt
    29161 tggctgggac tgatatttaa tttggacggc ggcttcgtgc ctttgcagtc tgccgtttta
    29221 ggtgcggttg ccggctatgg ttcgatatgg cttttatgcg cggtatataa actgattaaa
    29281 ggcgaaaccg gtatgggcaa cggagatttc aaactgattg ccgcattggg cgcgtggctc
    29341 ggcatatccg cattgcccgt gctgattttt gtttcctcgc tgatcggttt ggtcgcggca
    29401 atcgttatgc gcgtcgccaa ggggcagcat tttgccttcg gccccgcact gacagtttcg
    29461 ggctggataa tttttacggc aaacgattcc gtatggcggg cggtcaactg gtggctgacc
    29521 catccggtgc tgtaagatga cggtatgggt cggactgacc ggcggaatcg gcagcggcaa
    29581 atcggcagcc gcgcaatgtt ttgccgattt gggcgtgccg cgcatcgatg cggacgcggc
    29641 ggcgcactcg ctgacggctt cagacggcat cgccctgccg gaaatcaggc ggctgttcgg
    29701 cgacaccgtt ttcgacacac agggtttgtt gcggcgcgac atattgcgta aagaagtctt
    29761 tgcctcccca tcgcaaaaag ccttgctcga atccgtgatg ttgccgctga ttttctcaga
    29821 aatcaaaaag cagcaagaaa cctttaccga tgcggcttac ggcattgtcg aaattccgct
    29881 gctgacggaa aagcgtcaat ttatcagcct gatacggcgt gtcctgacca taagcgcacc
    29941 tttggaaaaa cgtatcggca gggtgatggc ccgcagcggg ctgacgcgcg gcgaagtggc
    30001 ggacatcatc agccatcagg catccgaatc cgaacgcctg ctgcttgcag acgatgtgct
    30061 gctcaatgac ggcagcctca aaagcctgcg tgagaaaaca atgcgcctgc acgcgtttta
    30121 ttcagggatt ttcgcctcaa aaccaacaca aggaaaacac aatgactgaa tcgcggcaaa
    30181 cacgccttca agtcaaatgt ccgacctgtc aaacagcagt agtatgggaa cccgaaaacg
    30241 cattccgccc cttctgttcg caacgctgca aactgatcga cttgggcgga tgggcagacg
    30301 ggaaatatac ggtttccggc caaacggaaa gtttgccgga aatatccgaa cccgacatgg
    30361 cataccgctg accgccccgc cttcccggca aacaccctga aagtcaaatg ccgtctgaaa
    30421 caaacacgct tcagacggca ttttcattct caaacctaat cgttggtatt tgccgttacc
    30481 tcttccaatg aagtaatgcc ctgcataact ttcaaaatac cggcccggcg caaatccacc
    30541 ataccctcct tataggcaac gtccaaaata tccacttccg taccgttgtt cataatcaca
    30601 cgctgcattt cttcgctgat gggcataacc tcatacacgc ccgcacgccc cttataaccc
    30661 tgcccccggc aacggtcgca accgacggcg cggtaaagtt tccaatcttt tgcaaggtcc
    30721 tcatcggtga agccgacttc cttcaaagca gaggcagacg ggcgttccac ttcctgtttg
    30781 cagctcgaac acagcctgcg taaaagacgc tgcgccataa tcaggctgac cgaactggca
    30841 atattaaacg gcgcgacacc catattcagc atacgcgaca acgtcgccgg cgcattattg
    30901 gtgtgcaggg tggaaaacac catatgccct gtttgtgccg ccttaatcgc aatatcggca
    30961 gtttccaaat cacgaatctc accgaccata atgatgtccg ggtcctgacg caggaaagac
    31021 ttcaaagcag cggcaaaagt caggccctgc ttatcattga cgttaacctg attgatgccc
    31081 ggcaggttaa tctcggcagg gtcttccgcc gttgcaatat ttaccgactc cgtattcaaa
    31141 atattcaaac aggtatagag cgacaccgtc ttacccgaac ccgtcggacc ggttaccagc
    31201 accatcccgt agggacggtg aatcgcttcc aacaacaatt ttttctgaaa cggctcaaaa
    31261 ccgagctggt cgatgttcaa agacgcggca tcggaattca aaatccgcat cacgaccttt
    31321 tcgccaaaca gcgtcggcaa tgtgctgaca cggaaatcga caggcttgcc gcccttttga
    31381 aaggtcagct gcatcctgcc gtcctgcggt atccgttttt cggaaatgtc caaacgcgac
    31441 attaccttaa tccgggaagc aagctgcccc cttaccgcaa tgggcggctg aaccacctcg
    31501 cggagctgcc cgtccacacg gaaacggata cgggcattgt gttcgtaaaa ctcgaaatgg
    31561 atgtccgatg ccccgctgcg caaggcatcc gacaaagtct tatggataaa cctcggaaca
    31621 gggccgtctt ctgcctcctc gttgtcgata tacagggtgt ggctttcctc ttcctcctgc
    31681 ccctccccaa gctcctgaag cagcgatgtc gaacgcgaac ccacccaatc gagcaaaccc
    31741 gccaactggt catcctcgac aatgaccaac tcaacctcaa tccctgcggc agaaacggtt
    31801 ttctgaattt gcggcatctg tgtcggatcg gaaaccgcaa aaaatacttt gtcgccccga
    31861 cggaaaaccg gcacacagtg gaactccacc atctgctcct ccgtcaacac ccccatcagc
    31921 accctgtggc gcggataatg acgcaaatca agaatcgaat aactgaacac cctcgcaatc
    31981 aatgccgcaa gcgacttggg cgaaatgaca ccgtctgaaa acagcatcgg caacacttcc
    32041 ttacccgcct gcgactcatt gtagtaatgc tcggcctgct caacagtaac cacctggttt
    32101 tgaaccagaa tcctcagcaa acctacgctc atacgacctt atccccaaat ttattcattg
    32161 ttatacctgt acagctt
//
LOCUS       DAWFBO010000005        28636 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFBO010000005 DAWFBO010000000
VERSION     DAWFBO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28636
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.005"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(159..971)
                     /locus_tag="WGO87_000171"
     CDS             complement(159..971)
                     /locus_tag="WGO87_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0391604.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTISAGREVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            1056..1928
                     /locus_tag="WGO87_000172"
     CDS             1056..1928
                     /locus_tag="WGO87_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0391605.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            complement(1985..3139)
                     /locus_tag="WGO87_000173"
     CDS             complement(1985..3139)
                     /locus_tag="WGO87_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0391606.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVLAWLSAVFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(3184..3552)
                     /locus_tag="WGO87_000174"
     CDS             complement(3184..3552)
                     /locus_tag="WGO87_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0391607.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            3726..4169
                     /locus_tag="WGO87_000175"
     CDS             3726..4169
                     /locus_tag="WGO87_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0391608.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            complement(4296..4916)
                     /locus_tag="WGO87_000176"
     CDS             complement(4296..4916)
                     /locus_tag="WGO87_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0391609.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(5274..6473)
                     /locus_tag="WGO87_000177"
     CDS             complement(5274..6473)
                     /locus_tag="WGO87_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0391610.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIEAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLSNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            complement(6886..8055)
                     /gene="prpF"
                     /locus_tag="WGO87_000178"
     CDS             complement(6886..8055)
                     /gene="prpF"
                     /locus_tag="WGO87_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0391611.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            complement(8408..11014)
                     /gene="acnD"
                     /locus_tag="WGO87_000179"
     CDS             complement(8408..11014)
                     /gene="acnD"
                     /locus_tag="WGO87_000179"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0391612.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRRPGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGREIRLKDIWPT
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGKAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSLARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIAAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(11092..11880)
                     /locus_tag="WGO87_000180"
     CDS             complement(11092..11880)
                     /locus_tag="WGO87_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ0391613.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPILLIFVALYFVFSPKLDGSKEGKARISFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIISMF"
     gene            complement(11886..12539)
                     /locus_tag="WGO87_000181"
     CDS             complement(11886..12539)
                     /locus_tag="WGO87_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0391614.1"
                     /translation="MRLVGNFKGGGATKLENEDVSKVDKSSHSGIALGAHTEAPYHAA
                     TKIFNNHSPAPSSLVLTARYNPLSEPTTVIPLQPILEKLHYLDLLALTTENFDFTRSE
                     TFTSGKGTGGSKVSILEFNNDGTIGVKYNSYRFTTNVHAPQSVKDALNRFNELINNSE
                     QYKINLQPNTCMIINNTLALHCRDVIQDNRRLLIRIFGYKNNIEYISLEDDPVLAQG"
     gene            complement(12756..12938)
                     /locus_tag="WGO87_000182"
     CDS             complement(12756..12938)
                     /locus_tag="WGO87_000182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391615.1"
                     /translation="MDKFQSIINLTADAALFVQNKATEIATIGGCQAKGVREIASNAG
                     KTLLEKYLNSEQKDEI"
     gene            complement(13071..14225)
                     /gene="prpC"
                     /locus_tag="WGO87_000183"
     CDS             complement(13071..14225)
                     /gene="prpC"
                     /locus_tag="WGO87_000183"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0391616.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTELCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSLLLYWYQYSHNGKRIEVES
                     DEETIGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(14310..15185)
                     /gene="prpB"
                     /locus_tag="WGO87_000184"
     CDS             complement(14310..15185)
                     /gene="prpB"
                     /locus_tag="WGO87_000184"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0391617.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDTMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(15811..17121)
                     /locus_tag="WGO87_000185"
     CDS             complement(15811..17121)
                     /locus_tag="WGO87_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0391618.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(17260..18438)
                     /gene="ftsZ"
                     /locus_tag="WGO87_000186"
     CDS             complement(17260..18438)
                     /gene="ftsZ"
                     /locus_tag="WGO87_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0391619.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(18557..19801)
                     /gene="ftsA"
                     /locus_tag="WGO87_000187"
     CDS             complement(18557..19801)
                     /gene="ftsA"
                     /locus_tag="WGO87_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0391620.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVTSCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(19887..20615)
                     /locus_tag="WGO87_000188"
     CDS             complement(19887..20615)
                     /locus_tag="WGO87_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0391621.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYAYDGLPEKESEE"
     gene            complement(20605..21519)
                     /locus_tag="WGO87_000189"
     CDS             complement(20605..21519)
                     /locus_tag="WGO87_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0391622.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(21632..23041)
                     /locus_tag="WGO87_000190"
     CDS             complement(21632..23041)
                     /locus_tag="WGO87_000190"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0391623.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGANVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(23195..24262)
                     /gene="murG"
                     /locus_tag="WGO87_000191"
     CDS             complement(23195..24262)
                     /gene="murG"
                     /locus_tag="WGO87_000191"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0391624.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(24266..25591)
                     /locus_tag="WGO87_000192"
     CDS             complement(24266..25591)
                     /locus_tag="WGO87_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0391625.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(25717..27054)
                     /locus_tag="WGO87_000193"
     CDS             complement(25717..27054)
                     /locus_tag="WGO87_000193"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0391626.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGKKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(27144..27416)
                     /locus_tag="WGO87_000194"
     CDS             complement(27144..27416)
                     /locus_tag="WGO87_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0391627.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            complement(27531..28613)
                     /locus_tag="WGO87_000195"
     CDS             complement(27531..28613)
                     /locus_tag="WGO87_000195"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0391628.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         6765 a         7900 c         7306 g         6665 t
ORIGIN      
        1 cgtgggaatg acggttcagt tgcgtaggac tgggtcgtcg aaaggggcgg attcgatgga
       61 ttcgatgaaa acggtagaaa tgtgggattg atggaaatgg caggctgaag cccatcctac
      121 agcccgctga ttccccggca ccgatgccgt ctgaaccttc agacggcatc caaaacccgg
      181 tatttcaagg cgggcaggcg gttgcggacg ctgttcaggc ggttggcatc gatgtctgcc
      241 gtaacgatgc cttcgccttc gggtaatacg tccaatacgt cgccccacgg atcgacaatc
      301 atgctgtgtc cgaacgtgcg ccgtccgttt tcgtgcaaac cgccctgtgc cgccgccacg
      361 acgtaacatt ggttttcgac ggcacgcgcg cgcagcagca gctcccaatg cgccttgccc
      421 gtcgtgtgcg taaacgcagc gggcagcatc aatacgtcaa acggcagctg gcggcggaaa
      481 aattcgggaa agcggacatc gtaacaaatg cccgccgcca ccgacacgcc ttctgccgac
      541 aagtgcggca cttcccgccc tgcgctgatg gtatcggctt cggcatagcg ttcgcccaaa
      601 ccggaaaagc cgaagaggtg cattttgtgg tacagccccg tccgtttgcc gtcgcgcccg
      661 tacaccaata aagtattcat gaccttgccc gcctcggggc tttgcagcgg cacggttccg
      721 ccgaacagca ccacgccgca ttctttcgcc gtttcgctca atgccgtctg aaagcgtccg
      781 ccgcccaaag gctcggcaag cgcgagtttg tcggtatcgt ttgcgcccat cagcacccaa
      841 tattcgggca gcagcaccca atccgcaccc tgctccgccg cccgtgcgac caggcgtttc
      901 atggcggcga cgttggtttc cggcgacacg cccgacacca tctgcacggc ggcaactctg
      961 attttgtcca tttcttctcc tttttcctgc cgaccttgcg gcatattccg ccgataggct
     1021 accatgacgg tactttgcga aaaccgttta cacaaatgcc cattttgaac catattgccc
     1081 tcatcggtgt agggctgatc ggcggttcgt tcgttctcga cctcaaaagg cagggactcg
     1141 tccgcaccgt taccggtatc gacaccgacc gcgacaacct cgaacgtgca ttggaacgcg
     1201 gcgtgattga ccgggcttcc gttgccatcg acgcggacag catcggcggt gcggacttgg
     1261 tactgattgc cacacccgtc gccaccgttc ctgccatttt gaccgcgctg cgccccgttt
     1321 tgccggaaca cacttggatt tccgatgtcg gcagcaccaa atcttcggtc atcgaagcct
     1381 tccgccgctg tctgcccgac cgcctgcacc gctgcatcgc cgcccacccg attgccggtt
     1441 cggacagaag cggtgcgcaa gccgcgcagt tcgggctgtt ccgccacaga aaactcatca
     1501 tcacgccaca cggcggcgaa cattcagacg gcattgcctt ggtagaaaac ctgtggcacg
     1561 cggtcggtgc ggaaatttat acgatggacg cgcaacgcca cgacgcggtt ttcgccgccg
     1621 tctcccatat gccccacctg accgccttcg cctatgtcca ccagattctc gaccaccccg
     1681 acggacagga atatctgaaa ttcgccgcca cgggctttcg ggacttcacc cgcatcgcct
     1741 ccggtcatcc cgccgtgtgg gcggacatct gccttgccaa caaagacagc ctgctgcaac
     1801 tggttcaagg cttgggcaaa cagttggacg ttttggcaaa catcctgacc accgacgacc
     1861 gcgaagccct gtaccgctat tttgaagaag ccaaaaccac acgcgaccgc tggctggacg
     1921 gcaactgacc gccctgccgt ctgaaaagca aaccgcccag acccgaaatc tgcggcggtt
     1981 tgtctcaacc gggcctgccg tccgaacgcg gacgaatcag ccaaggaata tacttccacg
     2041 catacaccaa aagcgcgagt gcaaacaaaa ccgaagaggt gcggatgctg tgcgtgtagg
     2101 cagtgccgga agaaaatacg gcaaccatac ggacggcggt tgccgccatc atcagccaaa
     2161 acgcaacggg aacggctttg ggcggcggat aaatcggatt gcccgtatga ccaagcgcgg
     2221 tacgcgccat catgcccaaa gtcagcacgc cgataccgcc gaccccgatc agatgcacac
     2281 ccagattgag gaaagcgggt ttgaaataag acgcgccgac cgcaatcagc cccaatccgg
     2341 taaacagata gccggcaaac agaatccaca gcatcggctc tttcaacacg ggtttatacc
     2401 accagcggta cacctgcacg gtaaaaatca cacctgccgc aaaggcaaaa acggcagaca
     2461 gccaagccaa cacaccgtgc gccatcagca tggcagtcag catgggcagc cacagcgaag
     2521 cctgcgccac ccatttcgga ctgggaatct gcggcacatt caagcgtttg gacgtaaaaa
     2581 acgaaataat ccgcgtacca atcagaccga taaaacccga caccatcacc aagcccgact
     2641 gcaatccgct caagagtccg cctaggttgc cgttgtgcag ctggacgtgg aacgccgcat
     2701 gcgtgccgcc caagacgaac agcgcgaaca cggcaacata gttgcgttga ttctgtgaac
     2761 ggataacggg caaagccatg cacaccgcgc cgtaccagaa aaacagcgta ccgagtatgc
     2821 cgcttgccga cgcaccccaa cccgggataa aggcggcaat ccgcgcagcc agccaaaaga
     2881 tagtcaagcc gaccagaacg ccgccccgcg tgggcggctg ccccgtccaa gtggcgacgg
     2941 cggtcagcag gaaggcgacg acgaccagtc cggcataacc ccaaatcatc tcatgcgcgt
     3001 gccaatagaa accggacagc tcgtgcgttc ccgtgtagcc gaaaccccac agcaatacgg
     3061 acaatgcgcc gtacagagcc gccagcgaat aaaacgggcg gaacgccatt gcccagacgg
     3121 ggtgcttggt aaatttcatg tgtgttcctt gtttatcatt aatatatttg ccgcaaaacg
     3181 gaatcatgcc ggcaaaaacg gttcggcggc gggaaacagc tcgcgttctt caaagcgcgc
     3241 gtggtcgcgc agggttgtgg caaaagcggt attccacgcc gcgttaccgt attcggggct
     3301 tgccatcatc tgccgcagtc gggcgtggtc tttctcgaaa cgttgtttca attcgggggc
     3361 gacattctgc caaattgggg caaacttggt ttcttcttcg cgaaaatggg tttccaattc
     3421 ggaaaaatgc ggttcgagtt cgtcccgatg cctttcttcc ggcgtccgca acagacgcac
     3481 gcacagggaa agcgaatggt ggtggtcacg cgaaagcccg ataagggcgg gatgtcgttt
     3541 caacggtttc atgattttga gttacaataa caaattctca aacggtagcg cggatgtttc
     3601 agacggcata ccgccgcaga caggctgcat tctaccgccc cgacggacag ggtttcaacc
     3661 attaaggtat tgtccgtgaa ccttaaaccg taccgataag aataacaatc cataccgaga
     3721 aaatcatgta tttgacacaa catacggact acgggctgcg cgtccttatc tacactgcca
     3781 tcaacgacga tgcgctggtc aacatcagta ccatcgccgt aacctacggc atttccaaaa
     3841 gccatctgat gaaggtcgtt accgcgctgg tcaaaggcgg cttcctccac agcgtgcgcg
     3901 gaaaaggcgg cggtctgcgg cttgccacac cgcccgaccg catcaacatc ggctcggtcg
     3961 tccgccacct cgaaccgatg cagctggtcg aatgtatggg ttcgaacaac gaatgcctga
     4021 ttacaccgtc ctgccgtctg acaggcatac tcggcggtgc aatgaagtcg tttttcacgt
     4081 atctggacgg tttcacgctc caagacctgc tcaacaagcc gacctacgac ctgctctacg
     4141 aaccgagaat tccgattgcg gtgcagtaat gcatcaaaat gccgtttcaa acggatattc
     4201 cgaccgagcg cgatacgcgc cggcatttcg gactgccacc ggcaaggcgc gcaatgccgt
     4261 ctgaacagcc gatgcttcag acggcatcgg tttccctaca aaccgaacac cgccgtatcg
     4321 ccacaacccc ggcgcaccag ttccgtgccg tcggtcatat cgacgacggt ggtcggctcg
     4381 gttccgcacc agccgccgtc aatcaccaaa tcgacggcgt gttccaaacg ctcgcggatt
     4441 tcataaggat cggtcaatgg ttcgccgtct tcgggcagca tcagggtgca gcttaaaagc
     4501 ggctcgccca attcccccag cagggcttgt gcaatggcat tatcgggaat acgcagcccg
     4561 atggttttgc gtttcgggtg cagcgtgcgc gccggcactt ccttcgtcgc ctgcaagata
     4621 aaggtataag gcccgggcgt ggcggcctta agctgacgaa actgtacgtt gtcgattttg
     4681 gcgtatgtgc ccaactcgct caaatcggcg cacatcaggg tcaggtggtg tttcaaatcg
     4741 atttttcgga tggagagtat gcgttccatc gccgccttat cgccgagttt gcaacccaag
     4801 gcataacagg aatcggtcgg ataaacgacc acgccgcctt tattgacgat ttcgaccgcc
     4861 tgcttgatga ggcgttcttg ggggttgtcg ggatgaatag cgaaaaactg tgccatagtt
     4921 tttttcctta tcgtcatgtt ttccgtattg tacgcccgaa attcaaaatc ccgtcatttc
     4981 cacaaaaaca gaaaatcccg tcattcccgc gcaggcggga atccggttcg ttcggtttcg
     5041 gtcatttcca acaaatccct gtcgctttgc gttgctggtt tcctgctttc gtagaaatgg
     5101 cggcggaggg tttctgtttt ccgacaaata tttatgttta caggatgaaa ttccaccgtc
     5161 cgcacaaaaa tgcaagataa caaaaaaatg aacaatgccg tctaaaatcc tcatcagcct
     5221 aatcgggcgt ttggggtttc agacggcatt gtgcggactc gtcccttttt tggctacaag
     5281 atgccgacaa gttcggcagt gtcgcaggca atcatcagtt cttcattggt cgggacaacc
     5341 aaaacagccg gagaagaatc ggtcgggctg ataatacccg aattgccgta gcgtttgtcc
     5401 atattggctt tagtgtcgat gtgcagaccc aagaaatcaa gataggaaac ggttttagct
     5461 cggatattac gtgagttttc gccaataccg ccggtgaaca cgagtgcgtc aacgccgccg
     5521 caggccacag ccatcgaagc gatgtatttg gcgaggcggt aggtcatgac ttcgagggcg
     5581 aggcgcgcgc cttcgtggcc ttcgtcggcg gcgatttcga gggtgcggca gtcgttggaa
     5641 agttcggaaa taccgagcaa acctgatttt ttgttcagca tttcatccac ttgggcaaca
     5701 tccatcccgg cgtgggaagt cagatagctg tatacgcccg gatcgatgtc gccgcaacgt
     5761 gtacccatta ccaaaccttc gatcggcgtg aaacccatac tggtatcgac ggatttgccg
     5821 tttttgatgg cggtaatgga tgcgccgttg ccgaggtggg caataatcat gcggatgtct
     5881 tccagaggtt tgcctagaat ccgcgcggct tcgggggcga cgtaacgcat actggtaccg
     5941 tggaaaccgt agcggcggaa ggcgtatttt ttacgcaact cgcgcggcac ggcataagtg
     6001 taggcacgtt cgggcatagt ttggtggaac gaagtatcca tcacgccgac attggacagg
     6061 ccggggaaat gttcctgcgc ggcgaggatg ccgctgatgt tggcggggtt gtgcagcggt
     6121 gcaagcggaa tgcaggcatt gagttcgtcc atgacggctt ggtcgatcaa aacagactcg
     6181 ctgtattttt ctccgccgtg ggcgatgcgg tggccgatgg cttcgatgcg gtcgtgcagt
     6241 ccgtgttttt ccagttcgtt caataacata cccaccgcgc cggcgtggca gtttcggccg
     6301 ctcaggggaa cttggcgttt gttgccgtct ttgttgaacg taatgacggc ttcgggcgtg
     6361 gtcaggcgtt cgccgaggca gcttaggacg acgctgccgc ttttgcggtc gataacggca
     6421 cctttgagcg atgaactgcc gcagttcaga acaagaatga gttggtcgga catgatgttt
     6481 ctccttgtag tttgtggttg ttttttggtg tggggtcgtc tgaaaaaggc agacatcagg
     6541 cgcaaggtgt ttcagacggc ctctttgggc aaagcgtatg ccgcattttc tatcgttatt
     6601 ccgtccccgc ctacgcgagg acaggctgtg gcgggaatcc agaatgtaga actcacgaaa
     6661 cctgttttcc cctaccaatc ctccatattg acaggtctag attcccgcct gcgcgggaat
     6721 gacggtaata cgggaagctg tcttttatcg gggtttccgt gttcttcctt gtgatttgcc
     6781 gccttccgcc acagggcaag ggaaaggaat ggtcagtgat ttgatgttta cttgtttttc
     6841 agacgacctg tttatggctc gcgggcaaac ccatgctacg tcaatttaaa aacaatcttc
     6901 cggcaccctg acccaacctt ccatcatcac gcgtgcgctg cggctcataa ccgctttggt
     6961 ggccgtccat tgtccgtcct gacattcggc ggctgcaccg acgcgcaatg tgccggaagg
     7021 atgcccgaag cgcacttctt tacgcgttcc gccgcctgcg gcaaggttga ccagcgtacc
     7081 gggcacggcg gcggcggtcg caatggcaac agaggcggta cccatcatcg cgtggtgcaa
     7141 tttgcccatg ctcagggcgc gtaccagcaa atcgatgtcg gcggcattca cggttttgcc
     7201 actggaggcg gtgtaatcgg cggcgggcgc gacgaaggcg actttcggcg tgtgcgcgcg
     7261 agcggcagct tcggatacgt cgctgatcag acccattttc agcgcaccgt aagcgcggat
     7321 tttctcgaat ttttccaaag ccgcggcatc gttgttgatg tcgtcttgca actctttgcc
     7381 cgtgtagccc aagtcggcgg cattcaggaa aacggtcgga atgcccgcgt tgatgagcgt
     7441 ggctttcaaa cggcctatat tcggcacatc aatttcatcg accaaattgc cggttgggaa
     7501 catactgcct tcaccgtcgg ctggatcaag aaattcgatt tgtacttcgg ctgccgggaa
     7561 cgttacgccg tcgagctcaa aatcgcctgt ttccaaaact gcgccgtttt gcatcggtac
     7621 atgggcaata atggttttgc cgatgttttt ctgccagatt ttgaccgtgc agatgccgtc
     7681 tgaaggaatc ttgcctttat cgaccaagcc ttgctcgatg gcaaatgcgc ccacggcggc
     7741 ggtgaggttg ccgcagttgc cactccaatc gacaaaaggt ttgtcgatgg aaacttgccc
     7801 gaaaaggtaa tcgacatcgt gatcggcgcg ttcggacttg tccaaaatca ccgccttgct
     7861 ggtggacgag ctggcgttgc ccaaaccgtc tatctgcttg ccgtagggat ccgggctgcc
     7921 gagtacgcgc aagaggattt tgtcgcgtgc gcttcccgct tcccgcgccg cctcgggcag
     7981 gtcggaacgt ttgaaaaaca cgccttttga tgtaccgcca cggtagtaaa cggcgggaat
     8041 tttaatttgc ggcatttcta gattctcctt atgtagcgtg ggctctgccc acgattttta
     8101 tagtggatta ggctgccact ccgaccaaag ccgtttgatt ccgcaaactg tcggggttcg
     8161 ccccaatcta cggctactgt gttgtgatac ggcagattat taaaccccgt cattcccgcg
     8221 caggcgggaa tccagattta tccgcacaga aactcatcgg ataaataagg tttcctcaat
     8281 tccactttct ggattcccgc ctgcgcggga atgacgattt acagtagatg cctgccatat
     8341 cgagaattac gtgaatgagc aaaatgttga acccgaccca cgctacgtct tgcttttcag
     8401 acgacctcta agccgcgttc ccttccaaaa aatcctgtgc aaaccgttgc aacacgccgc
     8461 cggcttcata taccaatact tcttctgcag tatcgaggcg gcaggtaacg ggaacttcga
     8521 cggtttctcc gtttttacgg tgaatcacga gggtcaggtc gcagcgcggt gtacgttcgc
     8581 cgataacgtc gtaggtttcc gtgccgtcca gttgcagggt gtggcggttg gtgccgggtt
     8641 tgaattgcag cggcaagacg cccatgccga tgagattggt gcggtggatg cgctcgaaac
     8701 cttcggcggc gatggcttcc actcccgcga gccgcacgcc cttcgccgcc cagtcgcggc
     8761 ttgagccttg accatagtcc gcaccggcaa tgatgatgag cggctgtttg cggttcatat
     8821 aggtttcgat ggcttcccac atgcgcatgg tttcgccttc gggttcgacg cgggcgagcg
     8881 agccttggcg cacgctgccg tcttcgtttt tcaccatttc gttaaacagt ttcggattgg
     8941 cgaaggtagc gcgttgggcg gtcaagtggt cgccgcggtg ggttgcgtaa gagttgaagt
     9001 cttcttcagg caaacccatt ttcgccaaat actcgcctgc ggcactgacg gccaaaatcg
     9061 cattggacgg cgagaggtgg tcggtggtga tgttgtcggg caaaatcgcc agcggacgca
     9121 tacctcttaa tgtgcgttcc cctgccagcg cgccttccca gtaaggcggg cggcggatgt
     9181 aggtggacat cggacgccag tcgtatagcg ggcttggggc tttttgcgct ttgccggtgt
     9241 cgaacatcgg tacatacaca tcgcggaact gctgcggttt cacatattca gcaacgacgg
     9301 catcgatttc ttcgtcggtc ggccagatgt ctttcaggcg gatttcgcgg ccgtctgcaa
     9361 cgccgagtac atcgttttca atatcgaaac ggatgctgcc tgccagcgcg taagcaacga
     9421 ccaacggggg cgaagcgagg aaagcctgtt tcgcatacgg atgaatacgg ccgtcgaagt
     9481 tgcggttgcc tgacagtacg gcggtggcgt acaaatcgcg gtcgatgatt tctttctgga
     9541 ttttcgggtc gagcgcgccg ctcatgccgt tacaggtggt acatgcgaag gcaacgatac
     9601 cgaagccgag tttttccatt tcaggcagca ggcctgcttc tttcaaatag atttcggcta
     9661 cttttgaacc cggggcaaac gaagatttca cccaaggttt gcgttgcaag ccgaggcggt
     9721 tggcattgcg tgccaacagc gcagcggcga caacgttgcg cggattggaa gtattggtac
     9781 acgaagtaat cgcggcaata atcactgcac cgtcaggcat ttggccgtct gaaggctctt
     9841 cgtaaggctt cgccagccct ttcgccgcca aatcggcggt cgcaaaacgg gcatgcgggt
     9901 tacttgggcc tgccatattg cgcgttacgc tgctcaaatc aaatttcaaa acgcgcggat
     9961 aaacggcggt tttcaaggca tctgcccata agcctgcggt tttggcgtag gtttccacca
    10021 atttcacctg cgcgtcgtcg cgtccggtca gtttcaaata atcaatggtt tgctcatcaa
    10081 tagcgaacat cgcggcagtc gcgccgaatt caggggtcat attggaaata gttgcgcggt
    10141 cgccgatgga caggcttctc gcaccttcgc cgaagaattc gacaaatgcc ccgaccacgc
    10201 gttctttgcg caagaactca gtcagtgcca acacgatgtc ggtggcagta atgcccggcc
    10261 ggcgtttgcc ggtcagctca acgccgacaa tatcgggcag gcgcatcatg gacgcgcgtc
    10321 ccagcattac ggtttccgct tccaatccgc ccacgcccac ggaaatcacg cccaatgaat
    10381 cgacgtgcgg cgtatgtgag tcagtaccga cgcaggtatc ggggaacgcc acgccgtttt
    10441 tgacttggac gacgggcgac attttttcta gattgatttg gtgcatgatg ccgttgcccg
    10501 ccggaatcac gtccacattt tcaaatgcgg tttttgtcca gttgatgaag tggaaacggt
    10561 cttcgttacg tctgtcttcg atttggcggt ttttgcggaa ggcatcgggg tcgtagccgc
    10621 cgcattcaac ggccagagag tggtcgacga tgagctgggt ttgcaccacc ggattcactt
    10681 tggcaggatc gccgcctttt tcggcaatcg catcgcgcag acctgccaaa tccaccaacg
    10741 cggtctgccc cagaatatcg tggcacacca cccgcgccgg ataccaagga aagtcgattt
    10801 cctgttttcc ctcaatcagc tgacccagcc agctttgcag cgtcggcaaa tcgactttgt
    10861 ccgcgcggtt gaccaaattc tccgccaaaa tgcggctcgt gtaaggcagc ttgtcgtaag
    10921 agccgggctt gatgtcctca cacgccgcac gcgcgtcgta gtattccaaa tccgtaccgg
    10981 gcagcggttt gcggtaacgt tggttggcag ccatgtcggt tctcctgggg atctgttttt
    11041 cttgtggttt gaggtttcag ccgacgtttt gaaggggtcg tctgaaaggg tttaaaacat
    11101 cgaaataatc atctgataca gcggatttct ctcgtctatc aacaatttca cagccatcga
    11161 aatgctgatg acaatcagca gcggcttaat cagcttcgaa ccgaagcgga cggcaaatct
    11221 cgcacctaaa ttcgcaccga caaacgcacc gaccgccatc gttgccgcaa tcgggaaaat
    11281 aatcgaaccg tgcagcagga ataccgatag cgaaccaaga ttgcaggcaa cgttcgccaa
    11341 tttggtgtaa gacatcgcgt tcaacagctt gcagccgagc aaaacaataa aggcaatcag
    11401 aaaaaacgag ccgacacccg gtccgaacac accgtcgtaa aaacccaaaa gcggtgcaac
    11461 cgtcagcccg aatagaaaaa aagatattct ggctttgcct tccttactgc cgtcgagctt
    11521 gggcgaaaac acaaaataca gcgcgacaaa tatcaacaaa atcggcacga ccgccagcag
    11581 aatatctttg gaaaccaagc tgaccgataa tgcaccgacc acgccgcctg caaacgatgc
    11641 tgccgcaatc gggagacctt tcttccaatc aatcaaacct ttgcgtgcaa aagaaaccgt
    11701 agccgaaaac gtagcagcgg ctgcttgcag cttgttggtg gcaattgccg acacgggagg
    11761 aatacctgcc aacaagagtg caggcagcgt aatcaaacca cccccacccg caatcgcatc
    11821 gataaatccg gcaatcatcg caaccaaacc caaagcgagt attatatata aatcttccat
    11881 gtttcttatc cttgagccaa tacaggatca tcttctaaag aaatatactc aatattattt
    11941 ttatatccaa aaatacgaat taacaatctt ctgttatctt gaataacatc acggcaatgc
    12001 aaagccaacg tgttgttaat gatcatacaa gtgtttggtt gtaagttgat tttatattgt
    12061 tcagagttgt tgattaattc attaaatcta ttcaaagcat ctttaaccga ttggggtgca
    12121 tgcacattag tagtaaatct ataagagtta tatttaacac caatagttcc atcgttatta
    12181 aattcaagta tagatacttt acttccccct gttccttttc ctgacgtaaa cgtctcacta
    12241 cgagtaaagt caaaattttc tgtcgttaat gccaataaat ccaaataatg caacttttcc
    12301 aaaataggtt gtaatggaat tactgttgtc ggttctgata gcggattgta tcttgcagtt
    12361 aaaacaagag atgatggtgc gggggagtga ttattaaaaa tctttgttgc cgcatggtaa
    12421 ggagcttcag tatgcgcccc taatgcaatt cctgaatgtg aacttttatc tactttgcta
    12481 acatcttcat tttcaagttt tgttgctcca cctcccttga aattaccaac aagtctcatt
    12541 attttcccat gattatcaat attataagca aatgcaaatt gctctactgt tgccaataaa
    12601 atctgattag atgcagccaa tttcaaacat tctaagtttt cttcaagctc ttccattttt
    12661 ggtaaagaac ttggaatgct aattattgaa ttatcaatgt ttttatcata aagattatta
    12721 aaaataacta cacaagtttt ttgatgaatg aatttctaga tttcatcctt ttgttctgag
    12781 tttaaatatt tttccaataa ggttttccct gcattacttg caatttctct tactcccttt
    12841 gcttgacagc cacctattgt tgcgatttcc gttgctttat tttgaacaaa caaagcagca
    12901 tcagcagtta aattaataat tgattgaaac ttatccattt aatacatacc ctttttatta
    12961 ggttaaattt ttaaacttat ttcagacaac ctttccaata aggcaaggtc gtctgaaatc
    13021 ctcagctttg cataccgaaa taaagaacaa actgctattg cattcttcaa ttatcgttct
    13081 tcaatctcca caaacgccaa atcttctgga cctgtgtagt ttgcgctcgg acggatgatt
    13141 ttgccgtctt tgcgttgctc gagaacgtgt gcgctccaac cggttgtacg ggaaattacg
    13201 aacagcggtg tgaacatagc ggtcggtacg cccaattttt ggtaggaaac ggcagagaac
    13261 cagtccagat tcgggaacat ttttttctct tcccacatca cgctttccaa acgttcggca
    13321 atgtcaaaga ggcgcatatc gccggtttct ttgctcaaac cgcgtgccac ttctttaatg
    13381 acaacgttgc gagggtcgga aatggtgtac accggatgac cgaaaccgat cacgatttct
    13441 ttgcggccga tgcgttcgcg gatgtcggct tcggcttcgt cggcattgcg gtagcgtttt
    13501 tgaatatcgt aagccacttc gttcgcgccg ccgtgtttcg gacctttcaa cgcgccgatt
    13561 gctccggtaa tgctggagta catatcagag cctgtaccgg cgatcacgcg ggcggtaaag
    13621 gtagaagcgt tgaactcgtg ttcggcatac agaatcagtg aaacgtgcat ggctttgatg
    13681 tgtgattcgc ttgggcgttt gccgtgcaac aggtgcagga aatgaccgcc gatggtctct
    13741 tcgtcgcttt caacttcaat gcgtttgccg ttgtgtgaat attgatacca gtacagcagg
    13801 aggctgccga ggctggcaat cagtttgtcg gcgatgtcgc gcgcttcact ttccggatgg
    13861 ctttcacgtt caggatgaac gcagcccagc atggatacgc cggtacgcat cacgtccatc
    13921 ggatgggtat gtgcaggcag gctttccaaa actttaatca cacggatagg caggccgcgc
    13981 atggatttga gcttggcttt ataagcggcc aactcgaatt tattgggcaa atgaccgtga
    14041 atcagcaggt gggcaacttc ttcaaactcg catttttgtg ccaaatccaa gatgtcgtaa
    14101 ccgcgatagc tcaaatcgtt gccggtacgg ccaacggtac acaattcggt attaccggcc
    14161 gcaacgccag aaagcgcaac ggatttttta ggtttgaaag tcggggtttg agtagtttca
    14221 gtcatggtat ttctcctttg tatttttatg ggttttattt ttcagacggc cgatgcggat
    14281 ttgttgaaag gccgtctgaa agcggtaaat catttttgaa acaatttatc cagtttttgc
    14341 tcgaaggcat gatagttcag atgctcgtac aattcggcac gggtttgcat agtgtcaact
    14401 actgcggcct gagtgccatc gcgcataatc gcttcgtaaa cattcagagc ggctttgctt
    14461 gctgcacgga acgatgacag cggatacagc accagcgaca cgccgttttc agccagctcg
    14521 ctttgggtat aaagcggagt ggaaccaaac tcggtaatgt tcgccaacac gggcactttc
    14581 accgcatctg caaattggcg gtacatgttc aaatcggtca tggcttcagg gaaaatcatg
    14641 tccgcaccgg cttcgacaca agcttgggcg cgttcgatag cggcatccaa accttctacc
    14701 gccagcgcat cggtacgcgc cataatcacg aagttctcat caacgcgcgc atctacggca
    14761 gctttgatac ggtcgaccat ttcatcttta gatacaatgg ctttgttcgg acggtggccg
    14821 cagcgttttt gcgctacctg atcttcgatg tgaaccgctg caacaccggc gcgttcaaag
    14881 ttgcgaatgg tacgggcaat attgaatgca ccgccccaac ccacatcgat gtccaccagc
    14941 agaggcgtat ccacgttgtc cgtaatgcgt cgtgcgtcga tcagcacatc ttccattgtg
    15001 gtaatgccca aatcagggat accgcaagaa caggctgcca cgccgccgcc ggacagatag
    15061 atggctttga aaccgctttg ggtggccaat cgtgcaaaat aagcattgac gcaaccggcg
    15121 acggcaagcg gattcgattc tttcacggct tggcggaaac gtgctccggc agagtgttga
    15181 ctcatcatat ttctccttta tagacttttt ttcagtatgg acaggcttcc atcacattcg
    15241 gacggcaaaa cacagccatc cgacgcgtcg ggcatcccaa tccaatcatt aaaaatatat
    15301 gggaaaaatt atcttattga tatttaaaac gaatcaaaga aaaacagcag accgttcgga
    15361 attatgcggc aaaaccgcag acaagaagaa aacaagggga ttattcagaa aaggggaaaa
    15421 catctgaatt ggtttcatag taatgttcct ttgtagtgtt atgtagtttt atttttcgat
    15481 aatctaaaac acaagactga aaatgtcaaa catttttaag gagactcatt acataatttt
    15541 aatattttat tttaattcca tgattaaact agattaattt tgaattattt gtttcaataa
    15601 aattgtcagg aaatcaagaa aaattttcga taatcaaaat cctgaaaact tataaaatat
    15661 tttatacata aaaacaatgt attattttta agcaatccga tgtattttat tatgtatttg
    15721 atttttatat aatttaataa aaattaaata tatcggcaaa caaaatgccg tctgaaaacc
    15781 tttcagacgg catttttaat atcgaatcaa tcagcccaaa taccagaatt tcagtgccca
    15841 caatacggca acaatccata ccataggcgg aacgtctttg gtgcggcggc ataaaagttt
    15901 aaccacggca tagctgatga agccgaaggc gatgccgtct gcaatcgaat aggtaaacgg
    15961 catgaagaca atggtcagga atgcgggtgc ggcttccgtc atatcgtccc aatcaatatc
    16021 cctcgcactg cggagcatct gcgtgccgac ataaagcagg gcgggcgcgg tggcaaaagc
    16081 gggaacactt ttcgccaaag gtgaaaacat caggcaggcg agcatcaata cgccgacggt
    16141 aaccgccgtc aggccggtcc gtccgcctgc cgatacgccc gccgcgcttt ccacataagg
    16201 cgtggtggaa gaagtaccca aagccgcacc tgccacaatg gcggtagagt ctgcaagcag
    16261 tgcgcgtttc aggcggggca gcttaccgtc caccagcagc ccggcacggt gggatatgcc
    16321 gaccagcgtt ccggtactgt caaatagatc gaccaagaag aagacgaaaa tcacactgac
    16381 catgctgacg gtaaacaggc cttcaaaatc catctgcata aaagtcggcg caatgctcgg
    16441 tacttcgccg atgatgccgt gaaattcatt caaacccatc aggctggcaa tgacggtaat
    16501 ggtcaagatg gtgatgatga ttgcgccttg aacgcggaaa tgtcccaata cgaccaccat
    16561 agcaaaaccg aacaatgcca acaacgcgga cggctgatga atatcgccca aaccgaccaa
    16621 ggttgccgga ttggcaacga taatgcctgc gcctttcagg gaaatcagtg ccaaaaacaa
    16681 accgataccg gcagcaatcg acattttcaa acccataggc agtgcgttga ccagcatttc
    16741 cctgacttta aaaaagctga acaggataaa aatcagaccg gagatgaaca ccgcacccaa
    16801 cgcaacctgc caaggcacgc ccataccctt aacgacggca aaggtgaaat aggcattcag
    16861 ccccatcccc ggtgcgagtg caatcggata gttgccgaca aaacccataa caaaacagcc
    16921 gatggcagac gcgatacagg tagcgacgaa taccgccccc atatccatgc cggtctcgcc
    16981 caaaatcaga gggttgacga taacgatgta gcacatcgtc aaaaaagttg tcaaacccgc
    17041 catcaactcg gtacgcaccg tcgtaccgtt tgccttcagc ttaaaaatcc cgtccaacag
    17101 tgtttgtttt gaagtgtcca tatctgaaag tctgtatcaa gtggaaaaat ctgaatccgc
    17161 cgaccgcaaa ccgtacgaaa ttgcaacatc ttgaaaaaca tacctcccaa accggacaaa
    17221 ccgattcggg aggcagcagg tttacgggca aacagcacat tatttgtctg aattgtgttg
    17281 acgacgcaaa atcgcaggga tttcaaagtc gtcaagtacg gactgattgt cgaaatccgc
    17341 agcggtaagg ttcatcgtgc ggataccgcg attggtgcgg atcatacctt cgacattgtg
    17401 gctttgctcc tgtttggacg gagcaaccgc ttctacctcc cttgccggaa caaaatcgac
    17461 cgcgcctttt tctttcagac cggtagcgat aatggtaatc cggatggcat cttcgctcat
    17521 ggtctcgtct tcagccgcac cgaatttgca ttccaaatcg ggatgcgcgc tttggttgac
    17581 gattttcatg acttcggaca actcggacat tttcaagcaa cccggagcag tcgtaatatt
    17641 gaccagcaca ccgcgcgctc cgtccaaggt tacatcgtcc agcagcggac tggaaatggc
    17701 ctggtcggtc gccatacgcg cacggtcgat accttgggca taacccgaac ccatcatagc
    17761 gataccgcgg ttgctcatca cggttttcac gtcggcaaag tcgaggttga tgatttcgct
    17821 cgggcaagtt accacttcgg aaatgcctgc gaccgcatcg cgcaatacat tgtcggcggc
    17881 acggaaggct tcgcgcatcg ttacgtcttc acccaatgca gtcatcagtt tgtcgttcgg
    17941 gatgatgatc agcgaatcga cgtgttcttt caactgttcc aaccctgcct gtgcgacatg
    18001 gacgcgctta ccttcatatg cgaacggtcg ggtaaccacg gcaacggtca gaatgcccaa
    18061 agacttggca atctcagcaa caaccggcgc ggaaccggta ccggtaccgc cgcccatacc
    18121 ggtcgtgata aacagcatat tcgcaccgcg aatggcttct tcaatggctt cccggtcttc
    18181 ctgggctgcc gcacggccga tatcgggatt cgcgcccgcg cccaaaccgc gtgtcagatt
    18241 cgtacccaac tggattctct tcgccgcatg gttttttgcc agagactgcg catccgtatt
    18301 ggcactgata aactccacac cgcgcacatt gttggcaacc atgttattga ttgcattgca
    18361 accgccgccg cccaagccga ttactttaat caccgcaggg ctgactgccg attctgccac
    18421 gtcgtaaaca aattccattc aaaaactcct gctcgcccca ttcagaggac ggtttaaata
    18481 aattattatt cattatataa gaagtatctt gctgctggca aaatacttct cacctgtcaa
    18541 acggcaatcc acctgttcag aagctgtttt caatccaccg tttcaatctt gccaacaaac
    18601 cgccgccccc ttccctctct tgcactgcac cgttttccgg ctgcggcaag tttccttcca
    18661 gcttgcatgc tgcatgaagc agcccgatag cggtagaaaa gcgcggtgtg cggacgcggt
    18721 cggacaaacc gcccatttct tggggtgcac cggtgcgtac aggcaaatcg aagatttttt
    18781 cggcaaattc cacaatcccg gtcatcatgg acacaccgcc ggtcagaaca atacccgcat
    18841 tcagcacttc tttggggaaa cccgattttt gcagctcgcc cagcactacg ccaaaaatct
    18901 cctgaatacg tgcactgatg attgctgcca aaaccttact ggaaacctga cgcgatgtcc
    18961 ggtcacccac gcccggaact tcaatcatct cacccaagcc ttccgtatcg catgatgtca
    19021 cgccataatg gattttaatg tactcggcgg catcgagagg tgttctcaac gatttggaca
    19081 aatctttggt aatcagatta ccaccggccg gaatgacgga cgtatggcgg atggcgccgt
    19141 tcatataaac ggcaatatcg gtcgttccgc caccaatgtc gatgacgcat acgccgaggt
    19201 ctttttcatc ttcagtcagc accgcctgcc cgcttgccaa cggctgaagc atgatctgat
    19261 cgcttttcaa gccgcaccgc tcgatacatt tttggacatt ctgcactgcc gtacttgcac
    19321 cggtaatgat gtgcacccgc gtatccaaac gcacaccgct cataccgata ggctccctca
    19381 cgccaagttg ggtgtcgata atgtagtctt gaaccacagc atcgagaatt ttttgatcgg
    19441 gcgggatatt gattgccttt gccgtttcaa tggcgcggtc gatgtctgcc tgcgtgactt
    19501 ccccgtcttt aattttaacc acaccttgcg aattgagact gcggatgtgg ttgcctgcga
    19561 tacctgtggt aacgtgggta attttggtat ccgccatcag ctcggcatca ttgaccgcct
    19621 gcctgatggc ttggacggtg gcatcgatat tggttaccat gcccgcgcgc aagccccgtg
    19681 aaggagcctg ccccaaaccg acgatgttga ttttgtcgtc atcttgaact tccccgatca
    19741 gtgcgaggac tttagacgta ccgatatcca gtacgctgat gtatctttgc tgctgttcca
    19801 ttgttcgtct gctcttaaaa ctgattgaaa tttgcgtcgc accgtttcag acggcacggc
    19861 cgtaatctgt ccgatacctg ttcccactat tcttcggatt ctttttcggg taaaccgtcg
    19921 taagcatagc ggactgaaaa tccgtcctta tacctcatat ccacatagga taaccgattt
    19981 ttatttttac gcaacagatg ctgccacgct tcggtaaaaa gccggaggcg tttcatctcg
    20041 ttttcccgtc cgagcctgac ggtgatgccg ttgtccaaaa cgacaatcca cgccgaacgt
    20101 gccgtatagg tcatctcttt gatgcccaaa ccctgttttg ccaaaacagt cgaaaattcg
    20161 tcataacggc ggagcatttc ggcagacgtt ccttccgcgc ctctgaatac cggcattccg
    20221 ggtctgtcca agcgggcttc aaaaacattg ccttcgccgt ccaccaaggc atggtcgccc
    20281 caacgcgcga ccggcttgcg ctcggtcagg acgacctcaa ccgtgtcggg aaaacggcgg
    20341 cgaaccatga ccgacgcaat ccacggatac cggcggtagg cctcctgtgc gccattgatg
    20401 tccgtcctca aaatattccc atggatgtat tctttcgcca aactgcccaa tgctttctta
    20461 tcggaataaa ccaggttgcc cttcagcgac acctgcttga cgggcagatg attcgaattg
    20521 taaaaccaaa ccagcccgga cgcagcaagc agcatcgcca tcatgacaag cagccagcgc
    20581 gtcagccgtt ccatcgcttc ggcattatcc cacatgtgcg gtcttcaaaa tttcaataca
    20641 taaatcggca aaacccacgc ccgtaacggc agcggatttc ggtactaaac tatggccggt
    20701 cataccgggc agggtgttga tttccaacag atagagtttg ccgtcggtat ctttgaggaa
    20761 atcgacgcgc acgcagcctt ccgcaccgat tgcctgcgcg ccgcgaaccg ccagttcgcg
    20821 catcaggctt tcttcggctt cggtcagatc ttccgaagga cattgataaa tggtgtcgtt
    20881 gcggttgtac ttggcttcgt agtcgtaaaa ctcggtcgcg gggatgatgt gtatgccggg
    20941 caggcctttg ccgttcaaca cagggcagga atattcgccg ccgccgataa accgttcggc
    21001 aatgatttcg ccctgaaggt gtttcaattc ttcgtaaacg cttttcagac ggccttttcc
    21061 tttgactttt accacgccta cgctgctgcc ttcggccgcc ggtttcacaa acatcggcag
    21121 gcccaatttt tcttcgacgg catcgaaatc agtgtcgtcg tgcaggacgg cgaactcggg
    21181 aacgggcagt cccaatgcct gccaaatcag tttgcagcgg tatttgtcca tgccgatggc
    21241 ggatgcggcg acaccgctgc cggtataggg aatgcccaac agttccaatg caccctgaac
    21301 cgccccgtct tcgccgtaag taccgtgaag gatgttgaat gccgtctgaa aaccttgtgc
    21361 cttcaattca gacaatgggg tttctttagg atcgaaggca tatgcgtcta tgcctttgct
    21421 ttttaaagca ttcaaaatgg cggtgccgct gtccagcgag atttctcgtt cgctggaaaa
    21481 accgcccatc aatacggcca ctttgccaaa attctgcatt gtttttgttc tttcctgatt
    21541 gctttatgct tgttgccaga ggtcgtctga aacctgattt gcggtttcag acgaccttta
    21601 tatgatgttc cgtctgtcag gcgggtgtgc ctcaaatctg tttcgacaat tccagcagcg
    21661 cggcggggac gcggttgatg cttcccgcac ccatattcaa cacgatgtcg ccgtcctgca
    21721 aaacgttcaa cagcatttcg ggcagatcgg caacgttttc gcagtaaatc ggctcgagtt
    21781 tgcccaacac gcggatggcg cgggcaagag cgcgggaatc ggcggcggca atcggctctt
    21841 caccggcggc ataaacttcg gtcagcacca gcgcgtcaac ggtattgagg actttggtaa
    21901 agtcttcaaa caaatcgcgc gtgcgggtat agcggtgcgg ctggaaggcg agtaccaaac
    21961 gtttttccgg atacgcgccg cgtgcggcgg caagggtcgc cgccatttcg acggggtggt
    22021 gtccgtagtc gtccaccaag agcgcggtcc cgccgtttgg caacttgatg tcgccgtatt
    22081 tttggaagcg gcggccgaca ccttcaaagc cgagcaagcc tttttggatc gcttcaaccg
    22141 atgcgccgac ttccagcgcc acgccgatgg ctgccaatgc gttcagcacg ttgtgtctgc
    22201 cgggcatatt cagcacgact tcaaacgacc cctgctcatg tcctttcatt tgaacatgga
    22261 cggtgaattt catttgcgcg ccgacgtttt cgatgtcggt ggcgtagatg tcggcggtat
    22321 cgtccaaacc gtaagtagca taaggtttgc tcactttggg caaaatcgcg cggacgtgtt
    22381 cgctgtcaat acacaaaaag gctttgccgt agaagggcat acggtggatg aaatcgataa
    22441 acgcctgatg cagtttttcg acgctgtgcc cgtaggtatc catatggtct tcgtcgatat
    22501 tggtaacgac ggacataatc ggtgtcaggt gcagaaagga tgcatccgac tcgtcggctt
    22561 cggcaacgat gtattcgcct ttgcccaagc gggcgttggt gcctgcggcg ttgagtttgc
    22621 cgccgataac gaaagtcggg tcaagtcctg ccgcgccgag gatggaggcg gtcagactgg
    22681 tggtcgtggt tttgccgtgc gtgccggcaa tggcgatgcc gtcacggaag cgcatcaact
    22741 ccgccaacat cagggcgcgc ggaataacgg gaatttgctg ctccaacgca gcgacaactt
    22801 cgggattttc ttttttgacg gcggtagagg taacgacgac attcgcaccg ttaacgtgtt
    22861 ctgcggtatg gccgggataa acttgaatgc ccaggctgcc caaatgctcg gtagcggcat
    22921 ttcgcgcctg atccgaaccg gaaactttaa aacccaaatt gtgcaagact tcggcgatgc
    22981 cgctcatgcc gacgccgccg ataccgacaa aatggatgtt ggtcactcga tttttcatca
    23041 taatgttgcg ttccggtgga tttcgatgcg taaaggcgtt attttaaagg gctgaccgtt
    23101 tgcgcgccat agttttctga caaatatata gcggattgaa ataaaaaaca tccatgccgt
    23161 ctgaacggct tttcagacgg catggttcgg cagtttacgc cgcacacgca atcgcggctt
    23221 cggcaacgtc gtccgcgctg tgcggcaatg ccaaagtacg ggcgttttct gcccatttga
    23281 gacatttttc acggttcagg ctgccgagga tttcggcgag tttttccgcc gtcaactggg
    23341 tttgcggcaa cagcaatccc gcctccgcct gcaccataaa acgcgcgttg gcggtttgat
    23401 ggtcatcaac ggcgtgagga tacggcacta acaacgcacc caatcccgcc gccgtcaact
    23461 cggcaatcgt cagcgcgccg gcacggcaaa tcaccaaatc ggcatcgcgg taggcggaca
    23521 ccatatcggt aataaattcc acgcattcgg ctttcacgcc cagcgcgtcg taatccgcct
    23581 gcaagctgcc cagcttgccc cgtcccgatt ggtggtacat ctgcggacgc gcattgtcgg
    23641 gcagcaaagc caatgcctgc ggtacggttt tgttcaaaac gtccgcgccc aaactgccgc
    23701 cgaccaccaa aattttcaga cggccttcac gcccttggaa gcgttcggca ggcacgggca
    23761 ggttgctaat atcggcgcgg acggggttgc cgaccaagcc gccttcgtgg ctgaacgctt
    23821 tcggaaaagc gtacaacacc cgcttcgccc agcgcgacag gtggcggttg gacaaacctg
    23881 ccacggcgtt ttgctcgtga atcacaatcg gcacgcctaa tagcttcgcc gccaaaccgc
    23941 cggggaaggt aacgaagccg ccgaagccga tgacgcactc gacacggtgt ttgcggataa
    24001 tccgctgcgc ttcgcggacg gtttgataca aagtaaacgg cagcatcagc ttgcgtttga
    24061 tgccgttgcc gcgcacgcct ttaatcgcca gcgtttccaa gcgtatgccg tattgcggca
    24121 cgatacgctc ttccatcgaa tccttgctgc ccagccaaat cacatgatgg ccgcgcgcgc
    24181 gcaacgaatc cgccaccgcc agcgcgggga aaatatgtcc gcccgttccg cccgccatca
    24241 gcataaaggt tttaccgccc atgatttact ccacccggta accgcgcatt ttccggcggt
    24301 tttcataatc tatacgcaac agcagcatca tgctgatcag catgaaaaag actgacgaac
    24361 cgccatagga catcaacggc agcgtcagac ctttggtcgg caaagcaccg atgttcacac
    24421 cgatattgaa gaaactttgg ataccgatcc aaatgccgat acccgaagcg atataggcgt
    24481 tgaaagtcaa acccaaatcg cgcgactgct taccgatgga aaacgcccgc accaccagcc
    24541 agccgtaaca gaatatcagc acgcacatcc cgaagaagcc gaattcttcg gcgatgatgg
    24601 caaaaataaa atcggtatgc gcttccggca gaaagccgcg tttgctcaaa ctcgcaccca
    24661 aacccatacc gaaccactct ccgcgcccga ttgccatcag agagtgggta agctggtagc
    24721 cggcaccctg cgggtctttc cacgggtcca aaaatgccac tacccgctgc acacggtagg
    24781 gagcggcggt aatcatcagc accatcccgc ccaagacgct gcctaccagg acgaaaaaat
    24841 atttccacgg caatcctgcc aaaaacagca ttccaacggc aatgacggta atgacgacaa
    24901 acgaaccgaa atccggctgt accattatca gcaccaaacc gaacgccacc agcataatcg
    24961 gcaggatgat cgcccggaaa cggccgtaca tttctaatgt ttcacgacgt gcctgcggat
    25021 tggtggcgga catgatcaga ttggccgtcc cccgccaaat cgactgccaa cccaaacttt
    25081 ccatgctgcg caacacttct tcacggcgcg tgaacaggct tgccaaataa aggatgaccg
    25141 ccagcttgaa cagctcggtc ggctggaaat tcaacggacc caaaggtatc caacgggtcg
    25201 cgccattgat ttcgcgcccg gcaatcaata cgactaccag caacaggccg gatagggcaa
    25261 aaatccacgg cacaagccgc cgccatgtcc tcatcctgca aagaaaccat aaaaaaccgc
    25321 tcgctatcaa gccggcaacg acgaaccccg cctgtctggt caaatagaaa aactgatcgc
    25381 cgccttcctt cgatgccaaa tacacagaag ccgaataaat catcagcagg ctgaacgccg
    25441 tcatcagcac caccatccac aaaagcggcg cgtcgaattt cctgccgtcg cgcacaatcg
    25501 gtctgtcgag cagcagagtg tggacaccgt cgcccacttt taccaatact tccgaaatct
    25561 tcaaaaaaac cacctgccag tctgtttgca cctgccgcaa agggcaaaaa tttcagacgg
    25621 cagacaatgc cgtccgaaca tacgatacat cccaaatcgg tattctaaat ctttacttgc
    25681 cgcccaacaa tgacggcgtt tgcatttcag acggcatcac aaagccttaa acgcttcgat
    25741 aaacacttcc gaacggtgcg cgtagccttt aaacatatca aaactcgcac aagccgggct
    25801 gagcagcacg atatcgcacg cttcggcttg ggcatatgcc gtctgaacgg cttcttccaa
    25861 agtggcgcag tcggtcatat tcagaccgca gccgtccaaa tcgcggcgga tttgcggcgc
    25921 atcaacgccg atcaggaaca cgccttttgc cttgccggca agcgcgtcgc gcagaggcgt
    25981 gaagtcctgc cctttgccca tgccgcctaa aatcacgaag agcggatttt gcaaaccggc
    26041 aatcgcggcg gcggtcgcgc cgacattcgt gcctttgctg tcatcaatga ataccacgcc
    26101 gtttttcttg ccgatttttt ccacgcggtg cggcaggcct tggaaggttt tgacgtgttc
    26161 aagcaatgct tcgcgcggca aaccgacggc ttcgcacaaa gccacggcag ccatgacgtt
    26221 ggtggcgttg tgcaagcctt gcaacggaat gtctttcgtg gcaatcaaat cttcattgcc
    26281 ttgtttcagg cggcctgtct cacgttccaa ccaaaaatcg gcttcgtgtt ccaacgaaaa
    26341 ccattttacc tcgcgcccgg cacgtttcat ggcacggcag aacgcatcgt ccgcattcaa
    26401 aacctgcaca ccgtcgccac ggaaaatctt ggctttggta tgcgcgtagt cgagcaagtc
    26461 gtcgtagcgg tcgagatggt cttcggaaat attcagtacc gtcgccgccg tcgggcgcag
    26521 gctttcggtg ttttccagtt ggaagctgga aagctccaac acccacacgt ccgccttttt
    26581 gccttcgcgc tgccattccg cctccaaaac cggcgtgccg atattgcccg cgataacggt
    26641 atccagcccg cacttgatac agagatagcc gaccaggctc gttaccgtgg ttttgccgtt
    26701 gctgccggta atcgcaatta ccttgtcgcc ccggcggttc acaatgtccg ccagcaattc
    26761 gatgtcgccc aacacgcgtc cgccgttttg cttgaacgcc tcaatatccg gctgacgctc
    26821 gctgatgccg gggctgagtg ccaaaatatc gaaaccgttg tccagcgcat ctttcagacg
    26881 gcctgtgtaa aacaccagcc cgtcaaacat cttaccgatt tgcgacacgc gctcgggctt
    26941 caactccgca tcataagcgg ccacctccgc gccgtttttg cgcagatagg caatcatgga
    27001 aatacccgta ccgccgagtc cggcgacgag gatttttttg ttttcaagcg tcattttggt
    27061 tttgtcctaa aacaaatcat attgggcagg tggggggatg tccgcccctg cccaagccgc
    27121 tttcagacgg catcgcgggc tgttcaataa cctgccttca ggcgttggta aatgtcgcag
    27181 ccgtcttggt ctccgttttg acaagcctta ccaaaccatt cttgagcgag cacaaggtct
    27241 tggcgcacgc cgcgtccttt gtaatacatt gcgcccaaat tgtattgggc tgctgcattt
    27301 ccctgtgctg ccgcctgcaa gttttcccga aaatccgata cgtcatccgc ccacaccgct
    27361 tggttcaagc ccaaggcaat cagggcggcg gcaagccatt tgactgtctg tttcatggtt
    27421 ttacttctgt tttagtataa gccgccgtta acgatagggc tgggcggatt gtcgccgcag
    27481 gtttattgcg cgttcaaatg ccgtctgaaa ggtgttcaga cggcataggt tcagcggatt
    27541 ttgagggtac tcaaaccgat caacaccaag acgatggtaa taatccaaaa gcggacgacg
    27601 acttgggttt ctttccagcc tttttgttcg tagtggtgat ggatgggcgc catcaggaag
    27661 atgcgttttt tggttttctt ataccagcca acctgaagca taacggatac ggcttctacg
    27721 acaaataatc cgcccataat gacgaggaca aactcttggc ggacgatgac ggcgacggta
    27781 ccgagcgcgg cacccaatgc caatgcaccg acatcgccca taaagacttg cgcgggatag
    27841 gcgttaaacc acaagaaacc gaggcacgcg ccgcacatgg cggtacagaa aatcaccact
    27901 tcgtttgcgc cggcaacgta aggtaattgc aggtattggg caaattgtga gtggccgctg
    27961 gcataggcga agatggcgag gccggcggca acgaggacga cggggaaggt cgcaaggccg
    28021 tccaagccgt cggtgaggtt gacggcattg gatgtaccga cgatggtcag gtaagacaac
    28081 accaaaaagc cgaccacgcc cagcggcagg gcgatttgtt tgaagaacgg gacaatcaaa
    28141 atattgttgg cggaattggc ggcaaggtaa aacaatgcca aactggcgat aatggcaacg
    28201 cttgactgcc acaccatttt gaatttggcg gacacgccgt tggggtcttt atagacgact
    28261 ttgcgccagt cgtcgtaaaa accgagtgcg cccgtggcga gcaatacgcc caagagaatc
    28321 cagatatacg ggtttgccca gttgccccac aacagggtgg acacggtaat ggcggtcaga
    28381 atcagcgaac cgcccatcgt cggcgtgccg tttttgacga ggtgggtttg cggaccgtcg
    28441 gtacgcactg cctgcccgca tttgagcgcg gtcagcctgc gtatcgtcca cgggccgaac
    28501 atcagggaaa acgctaaggc ggtcaacgcc gccatgacgg cgcggaatgt ggtgtattga
    28561 aaaatattca gaccggttaa ccagttgctg aaatgtgcga gccataaaaa catggggctt
    28621 cctttttttg ttttgt
//
LOCUS       DAWFBO010000006        25228 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFBO010000006 DAWFBO010000000
VERSION     DAWFBO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.006"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            1..539
                     /locus_tag="WGO87_000196"
                     /pseudo
     CDS             1..539
                     /locus_tag="WGO87_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            596..1669
                     /locus_tag="WGO87_000197"
     CDS             596..1669
                     /locus_tag="WGO87_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0391629.1"
                     /translation="MRPFFVGAAVLAILGALVFFINPGAIVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGIMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRAVLMNVNPIFFITVPAILTAA
                     VFVLYLLTFVPIFRANAFTDDPE"
     gene            complement(1716..3905)
                     /locus_tag="WGO87_000198"
     CDS             complement(1716..3905)
                     /locus_tag="WGO87_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0391630.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILGVQTTFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            4044..4826
                     /locus_tag="WGO87_000199"
     CDS             4044..4826
                     /locus_tag="WGO87_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0391631.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(5144..7078)
                     /locus_tag="WGO87_000200"
     CDS             complement(5144..7078)
                     /locus_tag="WGO87_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0391632.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASKVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIRTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(7144..8322)
                     /locus_tag="WGO87_000201"
     CDS             complement(7144..8322)
                     /locus_tag="WGO87_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0391633.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNSDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8548..8937
                     /locus_tag="WGO87_000202"
     CDS             8548..8937
                     /locus_tag="WGO87_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0391634.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFDNYNQAKLKQNKYFNITLSEVSPDHFTLQAAPDPMTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            9221..10267
                     /gene="adhP"
                     /locus_tag="WGO87_000203"
     CDS             9221..10267
                     /gene="adhP"
                     /locus_tag="WGO87_000203"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0391635.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(10432..13917)
                     /gene="smc"
                     /locus_tag="WGO87_000204"
     CDS             complement(10432..13917)
                     /gene="smc"
                     /locus_tag="WGO87_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0391636.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHTGQTIAGHEEHKGRLKQENQALNLPDEAETAAAQEAAALLQNR
                     QEHYEEQIIAAEEALHAAREAFQTASSRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSADEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQSLDEEYIVVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            complement(13996..14142)
                     /locus_tag="WGO87_000205"
     CDS             complement(13996..14142)
                     /locus_tag="WGO87_000205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391637.1"
                     /translation="MSWRIITRPAAGQYGKCRAKHRALAEPGFGALKMPSEGFRRHRG
                     CGMR"
     gene            14166..14888
                     /gene="lpxH"
                     /locus_tag="WGO87_000206"
     CDS             14166..14888
                     /gene="lpxH"
                     /locus_tag="WGO87_000206"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0391638.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSIAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            15157..16743
                     /locus_tag="WGO87_000207"
     CDS             15157..16743
                     /locus_tag="WGO87_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0391639.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16911..17114)
                     /locus_tag="WGO87_000208"
     CDS             complement(16911..17114)
                     /locus_tag="WGO87_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0391640.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            17230..17727
                     /locus_tag="WGO87_000209"
     CDS             17230..17727
                     /locus_tag="WGO87_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0391641.1"
                     /translation="MTALTLPEDIRQQEPSALLYPLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17809..19002
                     /locus_tag="WGO87_000210"
     CDS             17809..19002
                     /locus_tag="WGO87_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0391642.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQNFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(19406..20341)
                     /gene="hemC"
                     /locus_tag="WGO87_000211"
     CDS             complement(19406..20341)
                     /gene="hemC"
                     /locus_tag="WGO87_000211"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0391643.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVILQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20460..20975
                     /gene="ybeY"
                     /locus_tag="WGO87_000212"
     CDS             20460..20975
                     /gene="ybeY"
                     /locus_tag="WGO87_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0391644.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KSGYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            20977..21801
                     /locus_tag="WGO87_000213"
     CDS             20977..21801
                     /locus_tag="WGO87_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ0391645.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVIIGSLQFTVARADNRRLHTLMATRVK"
     gene            complement(21944..23323)
                     /gene="nhaC"
                     /locus_tag="WGO87_000214"
     CDS             complement(21944..23323)
                     /gene="nhaC"
                     /locus_tag="WGO87_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0391646.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHCYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23614..24837)
                     /locus_tag="WGO87_000215"
     CDS             complement(23614..24837)
                     /locus_tag="WGO87_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0391647.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         5777 a         7199 c         6533 g         5719 t
ORIGIN      
        1 cggcggatat gcggactgcc gagaaaaacc gtctgcatta ggttcatgaa acgcaagtcg
       61 gaagcgtcaa acaactgatt gcccattttg accggctgat tgacgaattg gacaaacaaa
      121 tcgacgacca cacccacacg cattttgacg gcaaagccca agtggcggag caaatcaaag
      181 gcatcggttc gataacgacg gctacgctga tggcgatgct gcccgaattg gggcggctgt
      241 cgcacaaacg gatagcgagt ttggccggca ttgccccgca cccgagggag agcggggaaa
      301 ccaaattcaa aagccgctgc tttggcggaa agtctgcggt acgtaaggcg ctgtatatgg
      361 ctaccgtggc agcgacacgt tttgaaccgc ttattcggga tttctaccaa cgcctgccgt
      421 ccgagggtaa gccgtataag gttgccgtta cggcatgtat gcgcaaactg ctgacgatat
      481 cgaatgcccg gatgcgtgat tattttgccg aaaacgatac cgccgaaaac ggtatctaaa
      541 cggcttgatt tgagttttgg tatttttgcc cgacggggtg aaaaatacag ttgctatgcg
      601 gccgtttttc gtcggcgcgg cggtgcttgc catactcggt gcgctggtgt ttttcatcaa
      661 ccccggtgcc atcgtcctgc accgccaaat tttcttggaa cttatgctgc cggcggcata
      721 cggcggtttt ttgactgcgg ctttgttgga ctggacgggt ttttcgggta acctgaaacc
      781 tgtcgcgact ttgatggcgg cattattgct cgccgcatcc gctatactgc ccttttcgcc
      841 gcaaactgcc tcgtttttcg tcgccgccta ttggctggtg ttgctgctgt tctgcgcccg
      901 gctgatttgg ctagaccgaa acaccgacaa cttcgccctg ctaatgttac ttgccgcgtt
      961 cactgttttt cagacggcat atgccgtcag cggcgatttg aacctgttgc gcgcgcaagt
     1021 gcatctaaat atggcggcgg tgatgttcgt atccgtgcgc gtcagtattc ttttgggcgc
     1081 ggaagccctg aaagaatgcc gtctgaaaga cccagtattc atccccaatg tcgtctataa
     1141 aaacatcgcc attactttcc tgctcttgca cgccgccgcc gaactttggc tgcccgcgca
     1201 aaccgccggt tttaccgcgc tcgccgtcgg cttcatcctg ctcgccaagc tgcgtgagct
     1261 tcaccatcac gaactcttac gtaaacacta cgtccgcact tattacctgc tccaactctt
     1321 tgccgccgca ggctatttgt ggacaggcgc ggcgaaatta caaaacctgc ccgcctccgc
     1381 gcccctgcac ctgattaccc tcggcggcat aatgggcggc gtgatgatgg tatggctgac
     1441 tgccggactg tggcacagcg gttttaccaa actcgactac cccaaactct gccgcatcgc
     1501 cgtccccatc ctcttcgccg ccgccgtctc gcgcgctgtt ttaatgaacg tgaacccgat
     1561 attcttcatc accgtccccg cgattctgac cgccgccgtg ttcgtgcttt acctgctgac
     1621 attcgtaccg atctttcggg cgaacgcgtt tacagacgat ccggaataaa aatgccgtct
     1681 gaaacggttt tcagacggca tcgtattgcc gataatcaag cctcctgcgg atcgacatcc
     1741 accgaccatc taattttgcc gtcgcggttt tgctgcaaca cctgcaccca caaactcacg
     1801 gcacggtgca aatcctgtcg ggacggggat tcgaggaaaa tttgcgcgcg ttcgcgttcg
     1861 gcgaggcgca ccatcagcat gggggcggcg ccaaactggg aaacgcttgc gggcaacagc
     1921 ggggcgaggg tttctttggc ggcattgaga aattccagcg catcggcaac acgcggcgcg
     1981 tccgcgcgga cggcggtctg aaaaccgaag ggcggcatgg cgaacatttg ccgttcgttc
     2041 aattcgtttt cggcaaacac gacgtagtcc tgcgctttga cggcggcgaa gacgggatgt
     2101 tcgggcagtt gggtctgtat cagcaccttg ccgggcttgt cggcgcgccc cgccctgccg
     2161 gacacctgca tcagctcggc aaacagcctt tccggcgcgc ggaaatccgc gctgtacagg
     2221 ctgccgtcgg cgttcaatac gataacgagg ttgagccgcg cgaaatcatg acctttggcg
     2281 agcatctgcg tgccgaccag aatatcgatt tcgttgtcgg cgatgcggcg gtacaaatcc
     2341 gcccagtcgt ttttgtgcgc cgtactgtcc ctatcgacgc ggacgacggc tgccttgggc
     2401 aggaaggcgc gcagggtttc ttcgacgcgc tgcgtgccgt ggccgacggc ggtcaggtct
     2461 tggttgccgc agtcggggca tttgtacggg atgggttcgc ggtggtcgca gtggtggcag
     2521 cgcagttggc gggcgcgttg gtgcagcacc attttggcgg agcagttcgg gcagccgaag
     2581 gtatgaccgc agtcgccgca aaacagcgcg ggcgcgaagc cgcgccggtt aaggtacacc
     2641 agcgacatac cgcctgcttc aaagttctgt ttcaaaagct gcaaggcttg cggcgagaag
     2701 ccgttgtcga gtttcagacg gcctacgttg agaatgtcca cttgcggcag ttgcgcggcg
     2761 gtatgggcgc gttcggtcag ttgcagcagg cggtacgcgc cactttgcgc cttgtgccag
     2821 ctctccaagc tgggcgtggc actgcccaac acgacggggc agccgccctg cttcgcccgc
     2881 cacaccgcca aatcgcgggc atgatagcgc aattcgttgt cttgtttgaa cgagccgtcg
     2941 tgttcctcat cgaccacaat cagcccgaca tcatccatcg gcgtgaacac tgccagccgc
     3001 gtgccgatca ccagcttcgc ctgccctaac atcgcgcgca gataatcctg cgtgcgcttg
     3061 cctgccgcca tctgactgtg caacacggcg gtcggcacgt cggcaaaacg gttttccacc
     3121 cgcttcaaaa gctgcggcgt gaggttgatt tcgggcaaca gaaacaacac ctgccgcccc
     3181 tgagccaaca ctttcgccat cgcatcaaaa tacacctcgg tcttgccgct gccggtgatg
     3241 ccgtacagca gaaacggctg gaagcgtccc aatgccgtct gaatggcatc ggaagccttc
     3301 tgctggtcag catttaatac aaattcagag tgcgaagcct gcccattaca cgaccttaaa
     3361 acgggcttcg ccgcttccgt cgtttcaatc caaccctgct ccgcccaatc ttcgattaat
     3421 ttcgccgcct gcgcgtttac ctgcttcaac gctgccatcg tcatttcgcc cgacagcagc
     3481 gcgtcccaca aagccgcttt tttgttgaac cgcgccggcg gcggcgtttg cgccctgccc
     3541 gcttcgttca acgcgtaaaa caagggcggc tgcggcattt ccaccgcgcg cgtttccttc
     3601 aaaccttgcg gcaatgcggc aaatactgcc tgcccggtcg gatagtggta ataacgcgac
     3661 gtaaacgcca acaaatcacg ccagctttcg ggcagcagcg gttcgtccga aaaggtcgtc
     3721 tgaacgccca aaatccgcgc cgtatccata tccggcgcaa tatccgtttc ccacaccatc
     3781 ccgaccacgg ttttattgcg gaaaggcaca agcacccgcg ttcccggagg aagcggatcg
     3841 gaatgggaat aagtcaaaag gccgtctgaa agcggcacgt ttacggcgat gcggtggtag
     3901 atcatacagg caggcaaatc atttttttgc gtattttaac aggcggaagc tattttcaga
     3961 cgataaattg agcctaggcg caatgcaggc gcaacagaga tttgatacaa ttccgctttt
     4021 ccaacacgcg caatcatctt tatatgaaaa ccaagttaat caaaatcttg acccccttta
     4081 ccgtcctccc actgctggct tgcgggcaaa cgcccgtttc caatgccaac gccgaacccg
     4141 ccgtcaaagc cgagtccgcc ggcaaatccg ttgccgcctc tttgaaagcg cgtttggaaa
     4201 aaacctattc cgcccaagat ttgaaagtgt tgagcgtcag cgaaacaccg gtcaaaggca
     4261 tttacgaagt cgtcgtcagc ggcaggcaga ttatctacac cgatgccgaa ggcggctata
     4321 tgttcgtcgg cgaactcatc aacatcgaca cgcgcaaaaa cctgaccgaa gaacgcgccg
     4381 ccgatttgaa caaaatcgac ttcgcctccc tgcctttgga caaagccatc aaagaagtgc
     4441 gcggcaacgg caagctgaaa gtcgccgtct tctccgaccc cgattgtccg ttctgcaaac
     4501 gcttggaaca cgagtttgaa aaaatgaccg acgtgacggt ttacagcttt atgatgccca
     4561 ttgccggcct gcaccccgat gccgcgcgca aggcgcaaat cttatggtgt cagcccgacc
     4621 gcgccaaagc gtggacggat tggatgcgta aaggcaaatt cccggtcggc ggcagcatct
     4681 gcgacaatcc cgtcgcggaa accacttcct tgggcgaaca attcggcttc aacggcacgc
     4741 cgaccctcgt cttccccaac gggcgcagcc aaagcggcta cagcccgatg ccccaactgg
     4801 aggaaatcat ccgcaaaaat caataatcca tccgcaaaac aaaaggcttg gaaaattcca
     4861 agcctttttt gcgggaaatg ccgtctgaaa accaatccgc actgaaagtt atagtggatt
     4921 aaatttatag tggattaaat ttaaaccagt acagcaagcc aacaaacaca tcctaaccca
     4981 ttgaatgatt gaaaataata aactgaacaa tacgaaacac accgccgtca ttcccacgca
     5041 agtgggaatc tagaacgtag aatctaagaa accgttttgt ccgataagtt tctgctccaa
     5101 aatgaccggt cctgcagctt caaacggcat ctttgtgcca acctcaatcc tgcgccaatg
     5161 cgtctatcgg attgagtttg gctgctttat tggcaggcat aaagccgaac gcgatgccga
     5221 ttccggtcga acaggcgacc gcgccgatga cggacatggc ggaaatgtcc atcgggaagt
     5281 cggttacaaa atgattgaac acgaggctga cggcggcgga caaacccacg ccgaccaaac
     5341 cgccgatgac gcagattaac accgcctcaa tcaaaaactg ctgcaaaata ttgccgcgcc
     5401 gcgcgccgat tgccatccgt atgccgattt ctttggtgcg ctcggtaacg gacaccagca
     5461 tgatgttcat cacgccgatg ccgccgacta ccaatgaaat cagggcgatg gaggaaatca
     5521 gcagcttcat cgtaccggtg gtgctttcga ctatctgcct gatgctgtcg ctgttgttca
     5581 tgaagaaatc ttccgtgccg tgccgcgctt tgagcagatc ggtcagccct ttttcggcaa
     5641 cctgggtatt ggcattgtct ttgattttga cggtgatgga gttggtgtgg ctctcgcctg
     5701 tgatttggtg catcaccgtc gtatagggcg accaaagcat cagcacgtcg gaattgccga
     5761 aagcgttttc gtcttttttc atcacgccga tgacggtcaa ggggcgtttc ctgaacaaaa
     5821 tggttttacc caacggatcc gagtccgcaa agagtttgtc tttgacattt tggtcgatga
     5881 cgacgacctg cgcgtcttct ttcacatcgt tttcgtcaaa cagccgcccc gtttccagct
     5941 tcagtccgcg cacgtcgaaa tattgttcgc ccacgccgta caaagaagcg gtcaggtcgg
     6001 tattgcggta agtcagcgtg ccgccgctcg aagtcatggg cgtggcggaa gcaacgtagc
     6061 tttgtttggc gatgattttt gcgtcgtcta tggtcagggt tctgattttg ccgctgcgcc
     6121 tgtcgccgaa gccgcgcccg gggaagatgc tgatggtgtt cgtccccatc gaactgatgt
     6181 cttcgaggat ttttttctgc gaaccgttgc ccaatgcaac gacggagaca accgaagcga
     6241 taccgatgat gatgcccagc atcgtcagaa gcgaacgcat tttgtgcgcc aatactgctt
     6301 gcaccgacat tctgaaggct tcgacaaact ggtcgtaata aaacgaccac gaggcttttt
     6361 cccgaatcct cccgaccttg cttgcgggga tttcgggatt tttcgaggtg tcggaaatga
     6421 tttcgccgtc ccggatttcg atgacgcggt tggcgttggc ggcaatgccg gggtcgtgtg
     6481 tgaccataat gacggtatgc cccgcttcgt gcagcctgcg gatgatttcc atcacgtttt
     6541 tgccgctggc ggtatcgagc gcgccggtcg gctcgtcggc gaagatgatt tcgccgccgt
     6601 tcatcagggc acgggcgatg gagacgcgct gctgctgtcc gcccgagagt tcgccgggct
     6661 tgttgccctc tttacttgcc aaacccaaat cctgcaacaa cttctccgcc cgcgcggaac
     6721 gctctttgcc gcccataccc atatacacgg ccggcagcgc aacgttgtcc cttgcggtca
     6781 gcgagcttaa gaggttgtag cgttggaaga tgaaaccgaa gcgttcgcgg cgcaatgccg
     6841 ccagttcgtc aggctgcatt ttggcagttt cgatgccgtc gattcggtac gaaccggaac
     6901 cggcggtatc caaacaaccg agtatgttca tcagcgtgga cttgcccgaa ccggactgcc
     6961 cgatgatggc gacaaaatcg cccttctcta tcgacaggct gatgtctttc aaaatatgga
     7021 cgcggttctc gccgctgccg aaatagcggt tgatgttttt acattcgatc aagctcataa
     7081 tattccgtaa atcaataaca aatgccgtct gaaaccgttt ccgtgttcag acggcatatt
     7141 cgtttatcgg cgcggcgggc cgcctagggc gcgttcgccg ctttcctgtt gctcggcggc
     7201 ggttatttcg gagatgacca ctttgtcccc ctctttcaac ccgcttttta cttcggtatt
     7261 catactgtct ctcataccgg tccggatttc gcgttccgcc gccttgccgt ccgcacccaa
     7321 cacgcgcaca aacgccttgc cgccgcgatt tttcacggtc agcgacggaa taatcagcac
     7381 atttttcacg ccgtcgattt caaccgtatt ctgcgtcgtc atccccgtgg cgagtttgcc
     7441 gtccgaattc ggcacaaacg aacgggcata atagtagacc gcattggaag ccgtatccgt
     7501 actgctgttg tagccgcccg acgacatcgt ggtcagcccg gggtcgacgc tgtcgagctt
     7561 cgccttaatc ggcgtatccg gttcggacaa aatcgtaaac gaaatatcct gccccgcctt
     7621 caccttggta atatcgccct cggcaatctg cattttgttc aacatcatat ccagattcgc
     7681 caattggaca atcgtcggcg tagactgcgc cgcgttcaca gtctgcccct cttccacgag
     7741 aatcgccacc accgtgccgt ccatcgttgc ggtaatgcgc gtgtagccca attccgactc
     7801 ggcggtattg atggaaattt tgctctgtct gattaaagcc ttcagctcgg caacattggc
     7861 tttggcggcg gcaaacgcat cctgcgcgct ttccaaatcc tctttggaag tcgcgttttc
     7921 cttccataac gccgcctgac gcttatattt cttctccgca ctgcccaatg caatctgtgc
     7981 cgacaccagc ttcgcctgat acgtttccaa cttggatttt tccgtattga gcgtattggt
     8041 ctgcgaggtc gaattgattt ccgcaatcaa atcgcccttt ttaacctgtt gcccgagttt
     8101 gacataaagt ttcttaatct gccccgatgc ctgcgcgccg accgatacca ggttggacgg
     8161 cgaaatctcc cctgttgcag aaaccgtccg gctgatgtcg ccgcgcctga ccgtttccgt
     8221 aatataagca gcctgcggct cgggcttcag ataagaccat ccgccccaaa ccgctgccgc
     8281 cgcgaccgcc gcaacagccg cccatttcat catttttgcc atgttttcaa ttctctgtat
     8341 gcgtcaatcc cataaatcgg aagcggattc taccgcaagt ccctgcccca tccaaggcgg
     8401 caaactgccg acaataaaaa taaccatttg ttttaaatgg cttatacaat ctgctttccc
     8461 gaaaatttca atccgtttac atattccgcc atttccgata cggcgttata atgccgtcga
     8521 ccgacaaaca aatggaaaca cattgctatg aaaaacgttc aaaaaggctt tacgctgctc
     8581 gagctgatga ttgccgtcgc catcctcggc atcctgacgc ttatcaccta cccttcctat
     8641 aaaacctaca tccggcgcgt ccgcctgtcg gaagtcagga cgaccctgct gcacaacgcg
     8701 cagaccatgg aacgttacta ccgccaaaaa gggacgtttg acaactacaa ccaagccaaa
     8761 ctgaaacaaa acaaatattt caacattacc ttaagcgaag tcagccccga ccacttcacc
     8821 cttcaggcag cccccgatcc gatgaccaac gacggcgaaa cctgcgtcgt tacgctcaac
     8881 gacggcggta ccattgccgc ttccggtaca aaccaatcct gccccggctt cgactaatcc
     8941 tccgccgaag cccgcaaacc cgtaaagatg ccgtccggac acagcttcag acggtatctt
     9001 tacgggtttg cacatccggt ttttcaaact tgaaacctgc cgcccctcat ccggcaaccg
     9061 ccccgatgcc gcgcctttcc aagatgttat aatataacat ataatctata tttttcaaca
     9121 acataccact tgacctgtga gatgcggaag cgtatgctta cccttatatc ggacaacatt
     9181 ccatttacag gtttttttgc cgtaactaag gagcgcaatc atgaaaatgc aggcagttgt
     9241 tgtgaataaa aatgtagcgg gcgatgtgga aatagtcgaa cgcgaggttc gcccgttgga
     9301 atacggcgag gcgttggtcg aagtcgaata ttgcggcgtg tgccacaccg acctgcacgt
     9361 tgcggcaggc gactacggcg aaaaaccggg ccgcgtgctg ggacacgagg gcatcggttt
     9421 ggttaaagaa gttgccgacg gtgtgaaaaa tctgaaagtc ggcgaccgcg tcagcatcgc
     9481 ctggctgttc caaagctgcg gctcgtgcga atactgcaat accggccgcg aaaccctgtg
     9541 ccgctctgta ttgaacgcgg gctacaccgc cgacggcggt atggcgacct actgcatcgt
     9601 gagtgccgat tacgcggtca aagtccctga aggtttggat cctgcgcaag cttccagcat
     9661 tacttgtgcc ggcgtgacca cttataaagc cattaaagtt tccagtgttc gtccgggaca
     9721 gtggattgcc atctacggcg cgggcggttt gggcaacttg ggggtccaat acgcgaaaaa
     9781 agtatttggc gcacacgttg tcgccatcga catcaacgac gacaaactgg cgtttgccaa
     9841 agaaaccggc gcggatttgg ttgtcaacgc cgccaaagaa gacgctgcca aagtgattca
     9901 ggaaaaaacc ggcggcgcac acgctgcggt cgtaaccgcc gtatctgctg ccgcattcaa
     9961 ctctgccgtg aattgcgtcc gcgcaggcgg acgtgtggtt gccatcgggc tgccgccgga
    10021 atcgatggat ttgtccatcc cgcgtttggt tttggacggc atcgaagtgg tcggctcttt
    10081 ggtcggcacg cgcaaagatt tggaagaagc cttccaattc ggcgcggaag gtttggttgt
    10141 gccgaaagtc caactgcgtg ctttggatga agcacccgcc attttccaag aaatgcgcga
    10201 aggcaaaatc accggccgta tggtgatcga tatgaaaaaa gaatgcggct gcggccatca
    10261 ccactgattt gacgtggcag tacacatcga aatgccgtct gaacgctgtt cagacggcat
    10321 tttttatgga tttggatttg attttaatct gttctgtttg aaataccgtc tgaaaaccct
    10381 tatcgcaaca cgtcattaaa aacggcaaga ttcagccgtt ctgcaacccg ttcaaacggc
    10441 ttccgccatt tccaacgcct gcttgatgtc cacggcgacg acgcgcgata cgcctttttc
    10501 ctgcatcgtt acgccgacga gctgctctgc catttccatc gtcaggcggt tgtgggagat
    10561 gtagaggaac tgggtttgcg ccgacatttc tttgaccagg ttgcagaagc gcgaggtgtt
    10621 ggcgtcgtcc agcggggcat cgacttcgtc caaaaggcag aacggagcgg ggttgaggct
    10681 gaacagagcg aacacgaggc tcatggcggt gagggctttt tcgccgccgg agaggaggtg
    10741 gatggtgctg tttttcttgc cgggcggacg cgccataatg gacacaccgg cggtcagtag
    10801 gtcgtcgcct atcattttga gagtggcttc gccgccgccg aacagggtcg ggaagaaggt
    10861 ttggactttg ccgttgacgg cgtcgaaggt ttctttgaaa cgcgctttgg ttttgtcgtc
    10921 gatttgggcg atggcttctt ccaaaatggt gatggcggcc tgcacgtctt cgctttggct
    10981 gcggtagtag ccgtcgcgtt cgcgcgcttc ttcgagttct tgcagggcgg cgaggttgac
    11041 cgcgccgagg gcttcgattt gttgcgagag gctgccgatg ctgctgttca atactttcgg
    11101 cgattctttc gccaacgctt cgagcgcatc caaatcggcg gcgcgttcgg tcaggttttg
    11161 atggtagcgt ttggcgttga tcagggcttc ctgttgttgc aacagggcgg tttgggtggc
    11221 ggcctgaagc tgtggcagct tggtttgcag ggtttgtacg cgggcgtatt gttccctacc
    11281 ctgctcctga atctgcgcga gtttctcctg tacgacaata tattcttcgt ccaaactctg
    11341 tacggcttcg ctcaactctt cgagcttgat gtgctgctcg tcgttttgga actcggtttc
    11401 ataggcaagg gcaagctctt gctggcgttc ctgccagtct agggtttgct gttcgagtcg
    11461 ggcgatttgc tgccggtagt tttgtttttg ctggttgagc ttgtggacgg cgacttcggc
    11521 cagcccgtat tggcggttgg cttccaacag ggcaagctgc gcctgtttca gacggccttg
    11581 ctgctcttgg cggctgtgtg cggtggtctg ctgttggtgt tcgagttcgg cggcggcttc
    11641 ctgcaaagta gcgatgtcgt ctgaaagccc gtcggacgtg tgttgcaaca cggtctgctc
    11701 ttccgccaac tgcgccagtt cgcgctcgat gtgttcgcgg cggatttgcc cttggttggt
    11761 gcgcgccaag agttcggcgg cgcgttgctg cgcttggctg tattggcgcg tgtgctgctg
    11821 ttgctgctgc atcaggtttt tatgttgcac ttcggacgag cgcacggcgg cttctgcctg
    11881 tttgaacgcg gcttcgtcag cggagagttc gggggctagg ttgtccaatt cggatgcgat
    11941 gccgtcgagg cgcgcttttt gggcaatcag gctttcctgc gcgggcttgg catagagcag
    12001 gacgctgacc ttatcgacct gatgaccttc gggcgtgagc cagatttgat gtgcgcccaa
    12061 atcgctttga tgcgcgaggg catagctcaa atcgggcgcg cacaatacgc cgtcgagcca
    12121 gtggtgcaat gccgtctgaa acggcggctg cgcttggatt tggttcagca atgcctgtac
    12181 gggcagggat tttttgatgc cgcctgagag gtcgtctgaa agccatgccg cctgcccctg
    12241 cggcaaaggc tcgggcggca cgaaaccttg cggcacggcg cgggcgtgca ggcgttcggc
    12301 aagaatgacg gacaaggcgt gctgccactc ggcgggcgcg gtgatgtgtt gccacagttg
    12361 cggcgcggcg gcgtggtcgg ttgcctgcca gaaatcggcg gcttcctgct gttgcgacag
    12421 gatttgcgac aacgcctgct gctgcgcctg caaggtaatg tgttgctgct tcaggctttg
    12481 gaagcggctc gatgccgtct gaaacgcctc gcgggcggcg tgtagagctt cttcggcggc
    12541 gatgatttgt tcttcgtaat gctcttgccg gttttgcaac aaggcggcgg cttcctgcgc
    12601 ggcggcggtt tcggcttcgt cgggcaggtt cagggcttgg ttttcctgtt tcagacggcc
    12661 tttgtgctct tcgtgcccgg caatcgtttg tccggtatgg gcaagctgct gctgtttcaa
    12721 cgccagttcg cggcggatgc ggtttgcctc gtcctgctgg gtttggaagg cggcattgag
    12781 cgcggtttgg gtttcttcca attcgggcag acgctcttcg tgttcggcaa cctgcatcgc
    12841 ccactccgcc agctcggttt gcttttcttc gacgtataac tcgttttctt ccagctgcac
    12901 gcggatttgc tgctgctctt gatgaatacg ctgcatctgc gcctgcgccg cctgcttgtc
    12961 gcgttcgatg cgttggtgca ggttttgctg atggcggatt tgttcttcca aacgggcaat
    13021 ctgctcgcgc aacacaccgc gtttgttgcc cagttcgtgt accgcctgct gctgcgactg
    13081 ttcggcggtc tgcaaggcgt gtacttcgtc gtttaacgcc tgaacctgcg cggcggtttc
    13141 gtcctgctgc gcctgcaaag actgatgctg cgcggtcgcc ttgtcggcgg cggcaagcga
    13201 ttgctgccat tgggcgtaat cgagcaaatc ctgttgctgg ttcagctgcg cggtcaggga
    13261 tttgtagcgt tcggcggttt ccgcctgttt ctccagcttt tccacctgac gcgctaactc
    13321 gttctgcaaa tcgcccaaac gctgcaaatg ctcgcgcgtg tctttcagac ggccttccgt
    13381 ctccttacgg cgttccttat atttggacac gcccgccgct tcctcgatat aggcgcgcaa
    13441 ttcctccggc cgcgcttcga tgatgcgcga aatcatcccc tgctcgataa cggcataacc
    13501 gcgcgcgccc acgcccgtac ccaaaaacaa atcggtaatg tcgcggcggc gcacggtctg
    13561 attgttgatg aaataagtcg attcgccctg acgcgtcagc tgccgcttga tgctcacctc
    13621 ggcatactgc ccccacgccc cctgcaaact gtggtcgctg ttgtcaaata ccagctccac
    13681 cgaagccctc ggcgcaggac ggcgcgtcgc cgcaccgtta aaaatcacgt cctgcatact
    13741 ctcgccgcga agctgcttcg ccgaagcctc acccaacacc cagcgcaccg cgtcaatcac
    13801 attcgacttg ccgcaaccgt tcggcccgat aaccgcgaca agctgccccg gaacatgaat
    13861 cgtggtcggg tcggtaaaag atttgaagcc ggagagtttg atgtgggtca ggcgcataat
    13921 ggtcggaaaa aataaaaaag aagcgtattt taacggaaat cccgccgcac caccccatat
    13981 cttgccggca aaaccttacc gcatcccgca cccgcgatgc cgtctgaagc cttcagacgg
    14041 catttttaac gcgccgaacc ccggttccgc caatgcccga tgcttggcgc ggcatttgcc
    14101 gtattgcccc gccgccgggc gcgttataat ccgccacgac atccgcccga tttggaaacc
    14161 tgcctatgaa acccgcctat ttcatttccg acctgcattt gagcgaaaag cagcccgaac
    14221 tgaccgcgct gttgctgcgt tttttacgtt cttccgccgc caggcaggcg cgggcggttt
    14281 acattttggg cgatttgttt gatttttggg tgggcgatga cgaagtttcc gagttgaata
    14341 cttcgattgc gcgtgaaatc aggaaattgt ccgacaaagg cgttgccgtg ttcttcgtca
    14401 ggggcaaccg cgacttcctg atcggtcagg atttttgccg gcaggcgggc atgacgctgc
    14461 tgccggatta ctcggttttg gacttgttcg gctgcaaaac cctcatctgc cacggcgaca
    14521 cattatgcac cgacgacaag gcataccaac gtttccgcaa aatcgtgcat cggaagcggc
    14581 tgcaaaaact gttcctgatg ctgcccctaa agtggcgcac gcgccttgcc gccaaaatca
    14641 ggcgtgtcag caaaatggaa aaacaggtca agcccgccga tattatggat gtcaatgccg
    14701 cctttaccgc gcggcaggta cgcgccttcg gtgcggaaag gctgatacac ggacacaccc
    14761 accgcgagca tatccatcac gaaaacggct ttacccgcat cgttttgggc gactggcata
    14821 acgactatgc ttcaatcctc cgcgtggacg gggacggcgc ggtattcgtg ccgctggaaa
    14881 aatactgaaa atgccgtctg aagcctttcg ggcggctttt tttacacgcc cctttcccac
    14941 actccctccg ccgcatcatc accatcctta acccgcatta aggggcgtta tcgataaaat
    15001 gcttgaccga ttacataaga ttacgtaaag tgtgcaaacg cataacaatt ggtcttacca
    15061 atttatcttt taaccaaaat atcgggtgta cgggatttga acgtctttcc aaacctcgac
    15121 gcgccttatg atgcaaatgc ctaaaaagga ttcgttatgg cacttttcct cagcatattc
    15181 cccatcgtcc tgctgatttg gctgatggtg aaaaaaaaca gtatgccctc ctacgtcgcg
    15241 ctgccgatta ccgcagtgct gatttacgcc atcaaacttt tctacttcga cgatgcgggc
    15301 atgctgctca acgccaccgc cgcttccggc ctcgtcaaaa cgctcacgcc gattaccgtg
    15361 attttcggcg cgattatgtt caaccgtatg atggaaacca cgggctgcat cgatgtcatc
    15421 cgcaaatggc tggcgaccat cagccccaac cccgtagcgc aactgatgat tatcggctgg
    15481 gcttttgcct ttatgattga aggcgcatcc ggcttcggca cgcctgccgc gattgccgcg
    15541 ccgattctga tgagcttggg cttcaacccg ttgaaagtgg cgattttcac tttggtgatg
    15601 aactccgtcc ccgtctcttt cggcgcggtc ggtacgccga cctggttcgg tttcgcaccg
    15661 ctgaacctga gtgccgaaga catcctcgcc atcggcaggc agaccggcgt aatgcacttc
    15721 ttcgcaggtt tcgtcatccc cgtcatcggc ttgggcttca tcgtaccttg gtctgaaatc
    15781 cgcaaaaact tgggcttcgt cgccattgcc gtcttctcct gcaccattcc ttatgtcgca
    15841 ttggcgatgg tcaacgaaga attcccgtcg ctcgtcgccg gcgcaatcgg cctgatggtg
    15901 tccgtattcg ccgccaacca aggctggggc ttgagcaaag accacgccaa agacccgaat
    15961 gccgaaaaag tgccgttcgc ccaagtcgcc aaagcactcg cccctttggg tatgctgatc
    16021 ggcatgctgg tggttacgcg catcaaacag ctcggcatca aaggcatttt gaccagcaaa
    16081 gaagaatggt tcagcttcca actgccgttt gatttgtcca aaatcaccgt cagcgactcc
    16141 ctgacgatta ccttcggcaa tattttcgga caagatgtca gcgcgtctta ccaaacgctg
    16201 tacgtcccgg cttggattcc gtttgtgctg accgtttgga tttgcatcct gctgtataaa
    16261 accaaattca aagatgcctg gacgatttat tccgtaactt tcaatcaaac caaaaaaccg
    16321 ctgcttgccc tgatgggcgc gctgattatg gttcagctga tgctggtcgg cggcgacaat
    16381 tcgatggtga aaatcatcgg taaggaattt gccgcaatgg cgggcgaaca ctgggtttat
    16441 ttctcgccgt atctgggcgc gatcggtgcg ttcttctccg gctccaacac cgtgtccaac
    16501 ctgactttcg gcccgattca gcagcaaatc gccttggata ccggcctgtc cgttaccctg
    16561 attctggcgt tgcagtccgt cggcggcgcg atgggcaata tggtgtgcct caacaacatc
    16621 atcgccgtat gtaccgtatt ggatgtgaaa aattccgaag gtgcgattat caagaaaacc
    16681 gttatcccga tggcgattta cggcgtgatt gccgtcgtcg cggcaatgat tttcttcctc
    16741 taggcgaaac gccaccatcc atagcggcag cacgcgggga acggaaaccg ccgttccccg
    16801 tctgggcggc gcgtcgggca gaatcccggt gccggaacag cccgccccgc caaacaaatg
    16861 ccgtctgaaa ccggaaaagg cttcagacgg catttttccg ccgtttgcat tcaggcgtaa
    16921 tgatgatgcg gcatcttgcc gtcaaggcgg tagcgcgtgc cgcaatacgg gcaggcaacg
    16981 ctgcccgatt ctccttcgca caaaggcaaa aacactctcg gatgcccgtt ccactgctcg
    17041 ttgtcgggtc ccgagcaata cagcggcaga ttttccggca acacggaaat ttcctgcaga
    17101 ttcagattgt ccatttgatt ttcctttgcg tggttggcgt gcgcccattt tacgccatcg
    17161 gcaggctaaa ggatattttc ggcgcaaagc cgcaatccgc tataatcccc acttttcaga
    17221 cggcatacca tgactgcgct tacccttccg gaagacatcc gccaacaaga gccatccgcc
    17281 ctgctctatc ccctcgtttc cgcctacctc gaacacaccg cccaaaccgg cgacgaatcc
    17341 ctctcctgcc tgtccgacga ccagcacacg ctgaccgcat tctgctacct cgacagccaa
    17401 gtcgaagaag gcggcttcgt gcaactcatc gcctccggtt acggcgaata tattttccgc
    17461 aacccgcttg ccgacagcct gcgccgctgg aaaatcaaag ccgtgccgaa agtcttggac
    17521 aaagccaaag ccctctacga acaacacggc aaaaccatcg aaacgctcgc cgacggaggc
    17581 gcagacatcg aatccctgcg caaacagttc cccgactttg aagaatggga cggcgcatac
    17641 tacgaagccg ccgaacaaga cctgcccctg cttgcagaac acatacagtc aaactgggaa
    17701 accttcgccc atatcgggca ggcgtgattg cgtctgtttc cagcccgtgt aaaacagcgt
    17761 aaaatcggca aacccgaatg accttccgtc cccatcaagg agcaagctat gttcttcaag
    17821 cacatcgaag ccgcccccgc cgatccgatt ctcggtttag gcgaagcgtt caaagccgaa
    17881 acccgccccg aaaaagtcaa cctcggcatc ggcgtttata aagacgcatc cggcgcgaca
    17941 cccctcgtca aagccgtcaa agaagccgaa aaacgcctgt tggaaagcga aaccaccaaa
    18001 aactacctca ccatcgacgg cgttgccgac tacaacgcgc aaacccaaat cctgctgttc
    18061 ggcaaagacc acgaaatcat cgccagccgt cgcgccaaaa cagcgcaaag cctcggcggt
    18121 acgggcgcat tgcgtattgc ggccgagttt gccaaacgcc agttgaacgc gcaaaccatc
    18181 tggatttcca atccgacttg gcccaaccac aacgccatcg ccaaagcggt cggtatccaa
    18241 gacaaacctt atcgttacta tgatgccgcc aaacacggtt tggattggga cggcatgatt
    18301 gaggacttga gccaagcgca aaaaggcgac atcgtcctgc tgcacggctg ctgccacaac
    18361 cctaccggca tcgaccctac gcccgaacaa tgggaaactc tggcaaaact ttctgccgaa
    18421 aaaggctggc tgccgctgtt tgactttgcc taccaaggct tcggcaacgg tttggaagaa
    18481 gatgcctacg gcttgcgcgt gttcttgaaa cacaatacag aattgctgat tgccagctct
    18541 tattccaaaa acttcggtat gtacaacgag cgcgtcggcg cgttcacttt ggtggccgaa
    18601 gatgaagaaa cagcagcccg cgcccacagc caagtcaaaa ccatcatccg taccttgtat
    18661 tccaacccgg cttcacacgg tgcgaacacc attgcgctgg tgttgaaaaa tgatgatttg
    18721 aaagcacaat ggattgccga actcgatgaa atgcgcggcc gcatcaaagc catgcgccaa
    18781 aaatttgtcg agttgctcaa agccaaaggt gcaagccaaa actttgattt cattatcgaa
    18841 caaaacggta tgttctcttt cagcggattg actcccgaac aagtcgaccg tttgaaaaac
    18901 gagtttgcca tttatgccgt ccgctccggc cgcatcaacg ttgccggcat taccgacgac
    18961 aacatcgatt atctgtgcga aagcatcgtg aaagtcttgt aaaacaaaag cgcatacgaa
    19021 atgccgtctg aaccttaacc agttcagacg gcatctgttt tttgttaaaa aacaataaac
    19081 cataacaatt tcaaacaagc taaaaaacat ctataaaatc aacccaatat gatgaactca
    19141 aataaaaaat acgaatatca tcattctcaa attcaaaatt tgaacgccac agtattatcc
    19201 gaagccacaa aaacctttcc gccgtcattc ccgcgcaggc gggaatccgg gaacccaatg
    19261 ctgcaagaat tgattagaaa caactgaaac cgagcaaact ggattcccgc ctgtgcggga
    19321 atgacgaaat aaggcgagta aaactgatga aacaaattga ttaaaaacaa taacttatat
    19381 tattttattt aaattttcga taaaatcaat tttccgtatt caatactgct ccaatcaatt
    19441 cccgcgcacc gtcgtccgcc aatttcttag cgacagcgcg tccgagcgcg tcggcatatt
    19501 cggcaggggc ttgcgcgtcc gcctgcaaaa tgaccgaacc gtcggggtgt ccgaccagtc
    19561 cgcgcaaggt cagcaagccg ttttcttccg tgcaatatgc ggccaaaggc acttggcagc
    19621 ttccgcccaa agcgcgcgcc aaagcgcgtt cggccgtaac gcaggcgtgt gtggtatcgt
    19681 ggtttagcgg ttttaaaacc tcatacaaat cttcgcggtg cgcggcaatt tcgataccca
    19741 atgcgccttg tccggcggca ggcaggctgt cggattccga caaaatcatg cggatgcgtc
    19801 cgtccaattc cagacgctgc aaaccggcgg cagccaagat aattgcgtcg tattcgccgt
    19861 tatcgagttt agacaaacgg gtttgcacat tgccgcgcaa aggtttgata accaaatgcg
    19921 gatagcgcgc acgcaactgg gcttcgcggc gcaggctgga tgtgcctacg acggcgcctt
    19981 tgggcatttc ttccaaacgc gcgtattggt tggacacaaa cgcgtcaaac ggattggcgc
    20041 gttcgccgat ggctgcaagc gcgaaacctt caggcaaatc catcggcaca tctttaatcg
    20101 aatgcacggc caagtcagcg cgcccgtcat ataaagcctg ttccaactct ttgacaaaca
    20161 agcccttacc gccgactttt gacaaagttt tgtccaaaat ctggtcgccg cgcgtggtca
    20221 tgcccaaaat ctcgacttcg caatcgggat acagggcttt cagacggcct tggatatgct
    20281 ttgcctgcca catagcaagc aggctttcgc ggctggcgat gacgagtttt ttcgggttca
    20341 tgttcaatgc gttcaaagaa tggaaacggc gtgaagtcta tcatattttg aacgtttctt
    20401 cagacggcac cgtcagatct atgttatatt tacacccaca aaacacgaaa cccgccacaa
    20461 tgaaacgcac caaaaaatac cctttcttaa cgttgcagcg gcaacgtttc catttgaact
    20521 ttgaaaacgc ctcttccgcc gccggcatcc cagccgaacg cgatttctac cgctgggcgt
    20581 ggtctgcctt gaaaagcgga taccgccgcg ccgacatcag cctgattctt ctggacgaag
    20641 aagaagcccg agcctacaac cgcgactacc gcggcaaaga ttacgccacc aatgtattga
    20701 gtttcgcgct caacgaaggc gaaatcctgc cctgccaagt ttcggaaaaa ctgtacggcg
    20761 atttgattat ctgcccgcaa gtggttttga aagaagccgc cgaacagggc aaaacgcccg
    20821 aacggcattt tgcccacctg accatacacg gcgttttgca cctgatgggc tacgaccaca
    20881 tcgaagacga tgaagccgaa ataatggaag ccgaagaaat ccgcctgatg ctggcggcag
    20941 gcttccccaa cccctaccaa gaggacgaat attaaaatgg acggtacgca gtcgaaaccc
    21001 aagtttttcg aacgcctgat ctcccgactt gccggcgagc ccgactccgc cgaagacgta
    21061 ttgaccctgc tgcgccaagc gcacgagcag gaagtttttg atgcggatac gcttttaagg
    21121 ttggaaaaag tcctcgattt ttccgatttg gaagtgcgcg atgccatgat tacgcgcagc
    21181 cgtatgaacg ttttaaaaga aaacgacagc atcgaacgca tcaccgccta cgttatcgat
    21241 accgcccatt cgcgcttccc cgtcatcggt gaagacaaag acgaagtttt gggtattttg
    21301 cacgccaaag acctgctcaa atatatgttc aaccccgagc agttccacct caaatcgata
    21361 ttgcgccctg ccgtcttcgt ccccgaaggc aaatcgctga ccgccctttt aaaagagttc
    21421 cgcgaacagc gcaaccatat ggcgatcgtc atcgacgaat acggcggcac gtcgggtttg
    21481 gtaacttttg aagacatcat cgagcaaatc gtcggcgaca tcgaagatga gtttgacgaa
    21541 gacgaaagcg cggacaacat ccacgccgtt tcttccgaac gctggcgcat ccacgcggct
    21601 accgaaatcg aagacatcaa caccttcttc ggtacggaat acagcagcga agaagccgac
    21661 accattggcg gcctggtcat tcaagagttg ggacatctgc ccgtgcgcgg cgaaaaagtc
    21721 attatcggca gcctgcaatt taccgtcgca cgcgccgaca accgccgcct gcatacgctg
    21781 atggcgaccc gcgtgaagta agcagagcct gcccgcaccg ccgtttctgc acagtttagg
    21841 atgacggtac ggacgttttc tgtttcaatc cgccccatcc gccaaacata aaaatgccgt
    21901 ctgaaaccgg aaaacaggtt cagacggcat ttcgcttacc cgcttatttt ttgctcaaag
    21961 tcagccccgt ccagccgaat aacagggtta aagccaaact caaatagcag aaaaaggcat
    22021 agggcagata ttcccagacc ggcacaccca gcgcatggct gataaatacg ccgcacacgc
    22081 tccacggaac aagcgggttg atgaccgtac ccgcgtcttc gagcgtacgc gagaggttgc
    22141 gggaatgtag tccgagctta tcgtaaacgg gtttgaacgt ttcacccgac aacaaaatac
    22201 tcaaatattg ctcgccgatc aggaaattaa ccccgaccga agtcatggca acgctgaatg
    22261 tggcacgtcc ggcattcgtc aaaaagctgc ggacggcatc cagcagggaa ggaatcgcgc
    22321 ccagcgcaaa cagcagcccg cccaaactca tcccgagaat cacgatggtt tgcgtgaaaa
    22381 acatactttc caagccgccg cgcgaaatga gtttggcgat gtctttaaac gcttcgcctt
    22441 cgagtttgta gccgccgtaa aaccacgcgc cgagctgacg cagatcgggc gtgctgtgca
    22501 gatacgttac ggcaacggcg gcaatgacgg taaagagcat agcgaccacg gcattgacgc
    22561 gcatcaatgc caaaacgacc aacagcgcaa acggaatcag cgaatagcag tgcaccaatc
    22621 ccgtggcttc aagctggctg cggaaggatt cgacgctgtt caaatcctgc gcggcgacgc
    22681 tgggaagaag ccacagcatc agtgccgcac tgatgagcca cgcgggaatg gtggtgtaca
    22741 tcatattttt gatgtgttca aacaggtcga taccgacaat ggacgcggaa atgcccgtcg
    22801 tatcggaaag cggggacatt ttgtcgccaa aaaatgcgcc cgaaacaatc gcgcccgccg
    22861 tcatcgccat atcggcctga aacgccgccg ccatacccat aaaggcaacg ccgacagtgg
    22921 cgcaggtggt caggctgctg ccgatggaca cgccgatgac ggaacacagc gcgaaggcgg
    22981 aaaaataaaa ataagtcggg gaaatcagtc cgaaaccgta atacatcagc gtcggaatcg
    23041 cgccgctcat catcagcgcg ctgaccatca gcccgatgaa gaaaaacagg taaatcgcgc
    23101 ccataccctg attcaacgcg cctatcatgc cctgctgcat atcgttgtat ttcaaaccgc
    23161 gcgccaagcc gtacaaaatc agcacgacga tggcggcaat aatggacata tgcggcagcc
    23221 actccaatga aatgatggta tagcccatcg cggcaatcag agcgacgacg acggcaagtg
    23281 cctcaccgcg cggcatatcg agtaaggatt tgaaagcgaa catgattctt tgcccgattt
    23341 gatataaagt taacggattc taacacacca taccgaatcc gcccgacgtt ttcagacggc
    23401 attttagcgt tttacaaaag ccggacagaa acaaaacggc aattttctca atttaaatta
    23461 atttttttga aatataattt tttatttttt tgtttgttga catataagac ttttatggat
    23521 tgaagtatta aaacctcggc acacacgccg caaacaccgg tacgcaaaaa tgccgtctga
    23581 aagatgttca gacgacattt ctcaagaaaa agattatttg tccgccctat atccgtacac
    23641 cgcaaaaaac acgatataca gataacagat gacggaaacg acaaacgaag acatcagggt
    23701 gtaagtatct gccacccagc cctgcacgac aggaacgacc gcaccgccga caatcgcggt
    23761 acacagtaca ccggaagccg cgttggtaaa ttttcccaat cctttggttg ccaaagagaa
    23821 aatcgtcggg aacataatcg agttgaaaaa accgatggca agcagcgacc acatcgccac
    23881 atccgcattg ccgctacccg tcgccatcgc gacggcaagc agtacgaccg cagccgatgc
    23941 gttaaacgcc aaataacggt tgggcgcgaa tttcgccatc accgccgaac cgaggaaacg
    24001 tccgaccatc gcgccgcccc aatagaacga caggtaatgc gcggcagaag catgatccag
    24061 ccctttcaga taacccaata cgttgaccat caacgaaccg atagacacct ccgcgccgac
    24121 atagcagaaa atgcctgccg taccgaacac gagatgtttg tattgccata cgctggtttt
    24181 gccgtcgtgg ttgtgcgcgc tttcctcggc ggcaattttg cgcgcgtcgg gcagccggat
    24241 cattttcacg aaaacggcaa ggataatcag cagccccgcc agtcccaaat agggaatctg
    24301 tacggaagaa atctgttccg ccttgctgac ggtttgggtt gcgtccgcca gaatcaggaa
    24361 cgcgccgatt tgcggcgcaa tggtcgtacc caaagcgtta aacgcctgaa ccagcgtcag
    24421 tgttgccgat tccttgccgg gtttcgccag cagggtaaca taaggattac cggcgacctg
    24481 aagcagcgtt acgccggaag ccaaaataaa caacgcgccc aaaaataccg cgtaagaatg
    24541 gctacccgca gcaggataaa acagcaggca tccgaccgcc gtcagcagaa agccgccgat
    24601 aacgccgttt ttgtagccga ctttgccgac aaaagccccc atcgggatgg acatcaccgc
    24661 ataggcggta aagaaacaga attggatcag catcgcctga acgtaagaca ggtcgaaaat
    24721 ttctttcaaa tgagggataa ggatgtcgtt catgcaggta ataaaaccca tcatgaagaa
    24781 cagcgtggtc aagacgacca atggggacgt atgattgttt tgtgattgcg cagacatgat
    24841 ttgcctttcg tggtgtcgga atattacccg tttcaagaaa atagaaattg acatttctta
    24901 acggcaatgc aaatcatact gatatttgaa cattttgcaa actttatcgg atgcaggcaa
    24961 tgcgccgatc tgaaattcaa gcagcagaaa ttaatatttt acagttgctt gcatagattt
    25021 taatagattt taaaaaattc cggcggactt gcaaaaaccg ttcccaataa aatgaaaatc
    25081 cgccgccgta tccggaaaat tcggaaatgc cgcatcgtgg catcaaaacc ggagcggaaa
    25141 gaatgtcgta tgcccgacaa aaaaggggca tacggctgtg ccgtattttt tgcaccgtcc
    25201 tccgcccttc ggcgggggcg tgaaaata
//
LOCUS       DAWFBO010000007        25210 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFBO010000007 DAWFBO010000000
VERSION     DAWFBO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.007"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            41..454
                     /locus_tag="WGO87_000216"
     CDS             41..454
                     /locus_tag="WGO87_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0391648.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            500..1129
                     /gene="nth"
                     /locus_tag="WGO87_000217"
     CDS             500..1129
                     /gene="nth"
                     /locus_tag="WGO87_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0391649.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPIADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1267..2766
                     /locus_tag="WGO87_000218"
     CDS             1267..2766
                     /locus_tag="WGO87_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0391650.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEASFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            2966..4486
                     /locus_tag="WGO87_000219"
     CDS             2966..4486
                     /locus_tag="WGO87_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0391651.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAIITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4543..5628
                     /gene="nagZ"
                     /locus_tag="WGO87_000220"
     CDS             4543..5628
                     /gene="nagZ"
                     /locus_tag="WGO87_000220"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0391652.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIAQLKELTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGLKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5853..5929
                     /locus_tag="WGO87_000221"
     tRNA            5853..5929
                     /locus_tag="WGO87_000221"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5887..5889,aa:Pro,seq:cgg)
     gene            complement(6264..6902)
                     /locus_tag="WGO87_000222"
     CDS             complement(6264..6902)
                     /locus_tag="WGO87_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0391653.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRARHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(6899..7270)
                     /locus_tag="WGO87_000223"
     CDS             complement(6899..7270)
                     /locus_tag="WGO87_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0391654.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QKRVQTLFSNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(7539..7961)
                     /gene="queD"
                     /locus_tag="WGO87_000224"
     CDS             complement(7539..7961)
                     /gene="queD"
                     /locus_tag="WGO87_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0391655.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGG"
     gene            complement(7968..8486)
                     /locus_tag="WGO87_000225"
     CDS             complement(7968..8486)
                     /locus_tag="WGO87_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0391656.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHTPHLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(8514..9173)
                     /gene="queC"
                     /locus_tag="WGO87_000226"
     CDS             complement(8514..9173)
                     /gene="queC"
                     /locus_tag="WGO87_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0391657.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            9278..9703
                     /locus_tag="WGO87_000227"
     CDS             9278..9703
                     /locus_tag="WGO87_000227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391658.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            9718..10938
                     /locus_tag="WGO87_000228"
     CDS             9718..10938
                     /locus_tag="WGO87_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0391659.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSMMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKRQ"
     gene            complement(10965..11246)
                     /locus_tag="WGO87_000229"
     CDS             complement(10965..11246)
                     /locus_tag="WGO87_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0391660.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(11325..11459)
                     /locus_tag="WGO87_000230"
                     /pseudo
     CDS             complement(11325..11459)
                     /locus_tag="WGO87_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(11458..11916)
                     /locus_tag="WGO87_000231"
     CDS             complement(11458..11916)
                     /locus_tag="WGO87_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391661.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(12481..12684)
                     /locus_tag="WGO87_000232"
     CDS             complement(12481..12684)
                     /locus_tag="WGO87_000232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391662.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
     gene            complement(13248..14183)
                     /locus_tag="WGO87_000233"
     CDS             complement(13248..14183)
                     /locus_tag="WGO87_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0391663.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCLDSIDYVDERKGVP
                     LAAMQHIFMDVRAAASHAYLFEHDLKKFKQYAYVAGKLGVLLSVNSTDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPAAEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(14186..16324)
                     /locus_tag="WGO87_000234"
                     /pseudo
     CDS             complement(14186..16324)
                     /locus_tag="WGO87_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
     gene            complement(16431..16694)
                     /locus_tag="WGO87_000235"
     CDS             complement(16431..16694)
                     /locus_tag="WGO87_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391664.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(16713..18143)
                     /locus_tag="WGO87_000236"
                     /pseudo
     CDS             complement(16713..18143)
                     /locus_tag="WGO87_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            complement(18342..18716)
                     /locus_tag="WGO87_000237"
     CDS             complement(18342..18716)
                     /locus_tag="WGO87_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391665.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            complement(18994..19371)
                     /locus_tag="WGO87_000238"
     CDS             complement(18994..19371)
                     /locus_tag="WGO87_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391666.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            complement(19380..>25210)
                     /locus_tag="WGO87_000239"
     CDS             complement(19380..>25210)
                     /locus_tag="WGO87_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0391667.1"
                     /translation="KHSTMVAVAETANSQGKGKQAGSSVSVSLKTSGDLCGKLKTTLK
                     TLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQTPNGRGLSHNRY
                     TQFDVDNKGAVLNNDRNNNPFLVKGGAQLILNEVRGTASKLNGIVTVGGQKADVIIAN
                     PNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGAAGWNDKGGADYT
                     EILARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTIALDTAALGGMYA
                     DSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADGTEASPTYLSIET
                     TEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPATTVLNAGHNLVI
                     ESKTNVNNAKGSANLSAGGRTTINDATIQAGSSVYSSTKGDTELGENTRIIAENVTVL
                     SNGSISSAAVIEAQDTAHIESGKPLSLETSTVASNIRLNNGNIKGGKQVVLMADGDIQ
                     AKASNLNASGNLYIHAGKDLDLNADKDLSTQSISLRADNTALISSNGNTLTAEKNLDI
                     QAGSLSVRQSNLQSSGGNVQMSATKGNISLNQSWINASQNIDTAALQGNIISDGLTAV
                     AEVGRVSLLANGNVDFNGLNTLIAEGDINAGSVGKGRLKMDNTDIYASAGDVKLVAGG
                     QLDLGNGTVNGGHISLDSNKGSMVVQDVHLNARASLKVDADQTLTINNSKLNSGHNTQ
                     INTNHGHMTLNQLDAHSRRHMSISAQGKGKGKDSGQILQNDQQNSKSTLAADGVLSLN
                     SSALQVLDNTTLRGGAINIKAGGGIIKRGHIDWETQDTATMRSAELKPLSGMMSIESG
                     GNNPLTVEPGNRIVSAGDLAVNHNGTFQISARAGNNGNPSAQTASVSAKGNIGIVAGE
                     VDIDAANMAAGKDLALVATKGNISLNSIRNTFSNYQLKTDKHNITQQLTDVEQELSKL
                     TSDPKYRKAQDLPQMLRRKYKRRDKVFGDSEARLRGLRAEINAADEAWAELQSPVKAL
                     LERKQLLQQALLTVSQPGSGHENQGSTLSGQNIKLLAAGGIRIQGSKVAATQQANIQA
                     AGFLPAPAAEELQEGRLQSAIDISGVFDTFEYGQQGSDKYGYAIFSRPSEISGKTGVT
                     LSAPYANENSRILLSTTDISSENGKIKIQSYGDQYYYARQSELYTFERRSYKTGKWYN
                     RKHITEVKEHKNAKPDAVNLSASQGIDIKSGGNIDAYATAFDAPKGSINIEAGRKLTL
                     YAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVMKTALPSRVVAESANLQSGWDTKL
                     QGTQFETTLGGATIRAGVGEQARADAKIILEGIKSSIHTETVSSSKSTLWQKQAGWGS
                     NIETLQLPSFTGPVAPVLTAPGGYIADIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNI
                     NWNQVQLAYDKWDYKQEGMTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGA
                     AAGTAAGTGVAAGTAATTGVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINH
                     TLKELGKSSTVRQAATAAVTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINST
                     AAASVHTAINGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGC
                     ASAVANKGKCRDGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGT
                     VAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLK
                     DEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYI
                     PNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLD
                     GQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
BASE COUNT         5816 a         6451 c         5972 g         6971 t
ORIGIN      
        1 caatccgtcg ccgattgtta cctgtttgag actgtttgca atgataccct cctacaccta
       61 cgccgccctc gccttttccg ccttcacttc cgccaccctc ctgcccggca catccgaagc
      121 cgcctttgcc ctgttcgtcc gccgctttcc cgaacacgca tacggcgcgt tgctgtgcgc
      181 cggccttgcc aacggattgg gcagtatggt ttcctattgg atgggacgtt tgctgccttc
      241 ccgaaaaatg ccgtctgaaa aaacactgaa tctgatgcgg cgtttcggta tttggctgct
      301 tccgtttgcc tggctgcccg tcgtcggcga cgcactgccg cttgccgccg gctggctgcg
      361 gttgaatccg tggacaagcg ggctgatgct ggttatcggc aaaacggcgc gttacgcctt
      421 cattctgtgg ggaatgcaat attacgccgc ctgaaccgtg cgttataatg ccgaccgcac
      481 aaaccgactg cacaaaaaaa tgaacagaca catccgccaa gaaattttcg aacgcttccg
      541 cgccgccaac ccccatccga ccaccgagct gaatttcaac tcccctttcg agcttttaat
      601 cgccgttctg ctttcggcgc aggcgaccga tgtcggcgta aacaaggcga cggcgaagct
      661 gtttccgatt gccgatacgc cgcaagcgat gctggatttg ggtttggacg gcgtgatgga
      721 atacacgaaa accatcgggt tgtataaaac caaatccaaa cacattatgc agacctgccg
      781 catcctgctg gaaaaataca agggcgaagt gccggaagac cgcgaggctt tggaatcctt
      841 gccgggcgtg gggcgcaaaa cggcaaacgt ggtattgaac acggcgttcg gacatcccgt
      901 tatggcagtc gatacgcata ttttccgagt gtccaaccga accaaaatcg cgccggggaa
      961 agacgtgcgc gaagtcgaag acaaactgat gcgcttcatc cccaaagaat ttctgatgga
     1021 cgcgcaccac tggctgattt tgcacggacg ctacacctgc aaggcgctca aaccgcaatg
     1081 ccaaacctgc atcatcaacg atttgtgcga atatcccgcc aaagcctgac cgccgccgcc
     1141 cttcaaaatg aaacgcttga aaattcggac ggacgttgta gaatttcaac ctatgccgtc
     1201 tgaagccgac aaaatgcgct tcagacggca ttaaagccgg taccggcatc cgacaaagga
     1261 caaaacgtgt tcaaaaaata ccaatacctc gctttggcag cactgtgtgc cgcctcgctg
     1321 gcaggctgcg acaaagccgg cagctttttc ggtgcggaca aaaaagaagc atccttcgta
     1381 gaacgcatcg aacacaccaa agacgacggc agcgtcagta tgctgctgcc cgactttgtc
     1441 caactggttc aaagcgaagg cccggcagtc gtcaatattc aggcagcccc cgccccgcgc
     1501 acccaaaacg gcagcggcaa tgccgaaacc gattccgacc cgcttgccga cagcgacccg
     1561 ttctacgaat ttttcaaacg cctcgtcccg aacatgcccg aaatccccca agaagaagca
     1621 gatgacggcg gattgaactt cggttcgggc ttcatcatca gcaaagacgg ctatattctg
     1681 accaatacgc acgtcgttac cggcatgggc agtatcaaag tcctgctcaa cgacaagcgc
     1741 gaatataccg ccaagctcat cggctcggat gtccaatccg atgtcgccct tctgaaaatc
     1801 gatgcaacgg aagagctgcc cgtcgtcaaa atcggcaatc ccaaagattt gaaaccgggc
     1861 gaatgggtcg ccgccatcgg cgcgcccttc ggcttcgaca acagcgtgac cgccggcatc
     1921 gtgtccgcca aaggcagaag cctgcccaac gaaagctaca cacctttcat ccaaaccgac
     1981 gttgccatca atccgggcaa ctccggcggc ccgctgttca acttaaaagg acaggtcgtc
     2041 ggcatcaact cgcaaatata cagccgcagc ggcggattca tgggcatttc cttcgccatc
     2101 ccgattgacg ttgccatgaa tgtcgccgaa cagctgaaaa acaccggcaa agtccaacgc
     2161 ggacaactgg gcgtgattat tcaagaagta tcctacggtt tggcacaatc gttcggtttg
     2221 gacaaagccg gcggcgcact gattgccaaa atcctgcccg gcagccccgc agaacgtgcc
     2281 ggcctgcagg cgggcgacat cgtcctcagc ctcgacggcg gagaaatacg ttcttccggc
     2341 gaccttcccg ttatggtcgg cgccattacg ccgggaaaag aagtcagcct cggcgtatgg
     2401 cgcaaaggcg aagaaatcac aatcaaagtc aagctgggca acgccgccga gcatatcggc
     2461 gcatcatcca aaacagatga agccccctac accgaacagc aatccggtac gttctcggtc
     2521 gaatccgcag gcattaccct tcagacacat accgacagca gcggcggaca cctcgtcgtc
     2581 gtacgggttt ccgacgcggc agaacgcgca ggcttgaggc gcggcgacga aatcctcgcc
     2641 gtcgggcaag tccccgtcaa tgacgaagcc agtttccgca aagctatgga caaggcaggc
     2701 aaaaacgtcc ccctgctggt catgcgccgt ggcaacacgc tgtttatcgc attaaacctg
     2761 caataaccca cataatttaa ccaaaatatt ttttaaatga taaaatgccc gtcaataccg
     2821 attaacgggc attttaaaaa atcagttaat tgatttcaaa taagtatttt ccggaacaga
     2881 aaatactttc gcccattttg ccaacaaaat ccgaaccgtt gcggacagaa accgccccat
     2941 ccggacgctt aacaccccca taaccatgca gctgatcgac tattcacatt catttttctc
     3001 ggttgtgcca ccctttttgg cactggcact tgccatcatt acccgccgcg tactgctgtc
     3061 tttaggcatc ggtattctgg tcggcgttgc ctttttggtc ggcggcaacc ccgtcgacgg
     3121 tctgacacac ctgaaagaca tggtcgtcgg cttggcttgg tcagacggcg attggtcgct
     3181 gggcaaacca aaaatcttgg ttttcctgat acttttgggt atttttactt ccctgctgac
     3241 ctactccggc agcaatcagg cgtttgccga ctgggcaaaa cggcacatta aaaaccggcg
     3301 cggcgcgaaa atgctgaccg cctgcctcgt gttcgtaacc tttatcgacg actatttcca
     3361 cagtctcgcc gtcggtgcga ttgcccgccc cgttaccgac aagtttaaag tttcccgcgc
     3421 caaactcgcc tacatcctcg actccactgc cgcgcctatg tgcgtgctga tgcccgtttc
     3481 aagctggggc gcgtcgatta tcgccacgct tgccggactg ctcgttacct acaaaatcac
     3541 cgaatacacg ccgatgggga cgtttgtcgc catgagcctg atgaactatt acgcactgtt
     3601 tgccctgatt atggtgttcg tcgtcgcatg gttttccttc gacatcggct cgatggcacg
     3661 tttcgaacaa gccgcgttga acgaagccca cgatgaaact gccgtttcag acgctaccaa
     3721 aggtcgtgtt tacgcactga ttattcccgt tttggcctta atcgcctcaa cggtttccgc
     3781 catgatctac accggcgcgc aggcaagcga aaccttcagc attttggggg catttgaaaa
     3841 cacggacgta aacacttcgc tggtattcgg cggcacttgc ggcgtccttg ccgtcgttct
     3901 ctgcacgctc ggcacgatta aaaccgccga ctatcccaaa gccgtttggc agggtgcgaa
     3961 atctatgttc ggcgcaatcg ccattttaat cctcgcttgg ctcatcagta cggttgtcgg
     4021 cgaaatgcac accggcgatt acctctccac actggttgcg ggcaacatcc atcccggctt
     4081 cctgcccgtc atcctcttcc tgctcgccag cgtgatggcg tttgccacag gcacaagctg
     4141 ggggacgttc ggcattatgc tgccgattgc cgccgccatg gcggtcaaag tcgaacccgc
     4201 gctgattatc ccgtgtatgt ccgcagtaat ggcgggggcg gtatgcggcg accactgctc
     4261 gcccatttcc gacacgacca tcctgtcgtc caccggagcg cgctgcaacc acatcgacca
     4321 cgttacctcg caactgcctt acgccttaac cgttgccgcc gccgccgcat cgggctacct
     4381 cgcattgggt ctgacaaaat ccgcgctgtt gggctttggc acgacaggca ttgtattggc
     4441 ggtgctgatt tttctgttga aagataaaaa acgcgccaac gcctgacccc tttccctgtt
     4501 cagacggcat atgccgtctg aagctttttg gagcaaaccg aaatgactgt tccccatatt
     4561 ccgcgcggcc ccgtcatggc agacattgcc gccttccgcc tgaccgaaga ggaaaagcaa
     4621 cgcctgctcg atcccgccgt aggcggcatc atcctcttcc gccgcaactt ccaaaatatc
     4681 gcacagctca aagaactcac cgccgaaatc aaagccctgc gcacacccga actcattatc
     4741 gccgtcgatc acgaaggcgg cagggtgcaa cgtttcatcg aaggcttcac ccgcctgccc
     4801 gcaatgaacg ttttagggca aatttgggac aaagacggcg cgtccgccgc cgaaaccgcc
     4861 gccggacaag tcggccgggt gttggcaacc gagctttccg cctgcggcat cgatttgtcc
     4921 ttcacgccag tcttagattt ggactgggga aactgccccg tcatcggcaa ccgcagtttc
     4981 caccacaatc ccgaagcggt cgcccacctc gccctcgccc ttcaaaaagg tttggcaaaa
     5041 ggcggtatga aatcctgcgg caaacatttt cccggacacg gatttgtcga aggagacagc
     5101 catctggtct tgccggaaga caggcgcagc ctgtccgaac tcgaagccgc cgacctcgcc
     5161 cccttccaca ttatgagccg cgaaggtatg gcggcggtta tgcccgccca cgtcgtttat
     5221 ccacaagtgg acacaaagcc cgcagggttt tccgaaatct ggctcaaaca aattttgcgc
     5281 cgcgatatcg gattcaaagg cgtgattttc tcggacgatt tgactatgga gggcgcgtgc
     5341 ggtgtcggcg gcctcaaaga acgcgcccgc atttcctttg aggcaggttg cgacatcgtc
     5401 ctcgtctgca accgtcccga ccttgtggac gaactgcgcg aagatttccg cattcctgac
     5461 aatcccgctt tggcgcaacg ttggcaatat atggccaaca cgctcggcag tgccgccgcg
     5521 caagccgtta tacagacggc ggatttccaa gcggcgcaag cctttgttgc cggtcttgcc
     5581 tcgccgcaag acacggcggg cggcgtaaaa gtcggagagg ccttttaaat cggctgcgtt
     5641 tcaacaatct gccgatttca accgtttgtc agggaaaggc aatgattttt tcggtcaggt
     5701 agaaagttat cttattgaat tacttggaga taaggatgct tatcaacact gtattcaaac
     5761 acagcccttt caatccgccc gacacgcggc aaaaaggcaa aacggtatag gcatgccgta
     5821 ttttttgcgc tataatcacg ccttacttaa ttcggaatgt agcgcagccc ggtagcgcac
     5881 ttcgttcggg acgaaggggt cggaggttcg aatcctctca ttccgaccaa atccaaaccg
     5941 cctcggcatc tattgccgga gcggttttgc cttttggcgt caacagaaaa atgccgtctg
     6001 aaacagaatg ctgtttcaga cggcatttcg ttttccctaa ccctgccatt tgcgcgcact
     6061 ggcacagagt atcggcaagg ttcaaaagac catcggaatt taggaagccg ggcttgtacc
     6121 caacggcagc atcttattca gtaaggctcc gtcctgattg ggcggtggaa atgccggcat
     6181 ttccacgcag aagcggcaca ctttccccgt atgtcaaaca tcggtaaaac cgtacgccgt
     6241 ccctttgagt tacactgctt aaactactct attcccgccc atttgtgcgt ctgcacgctc
     6301 aactgccaat gcacggatgc gtcggggcga ctgtttaaaa taccgatttg gcggatggtg
     6361 tcgtagatgt tcatcgcacc gtcttgctca cagggcgaaa ggtagtaatg gcgcgcacgg
     6421 attttgcgtt ccatattttc gcagaacgca acgacatcgc cgtctgccac gatgcgcact
     6481 tcgtcggctg tttcgataca gcttttttcg tatttggcgg catagcaggc tttggggctg
     6541 gtggcgacat agtcgatttg cggcggcgcg ggattgagtc cgttggtttc gatacagagg
     6601 aaataacctt ccgccttgag catgtccagc agcatatcga gatgcggctg gatggtcggt
     6661 tcgccgccgg tgatgatgat gttgcgggcg gcataggttt tcagacagcc taagatgtcg
     6721 gacaggctca tcatgccgaa tgtcaaataa tcggtatcac accagccgca ggcgaggttg
     6781 catttgccca agcggacgaa aacggcgggt atgcccgtgt tccagccttc gccttgcagg
     6841 ctttcaaaga tttcgacgat gcggtattgg ggattttcgg gggcaacatt gatttttttc
     6901 ataccgccaa caccgccgcc gctgccaaaa atgcgggcaa accttgtttg acgagtatgc
     6961 ctttgttgcc ggaagagaac gcgccccacg cggcggcaat caggacgaag ccgagaaaca
     7021 gtaccgtcgc gccgtacacc gcgttatccg gcgcggcaaa ccgcgaccac accagcccga
     7081 tgccgagaaa gccgttgtac agcccttggt tactgaacag ggtctgcacg cgcttttgtt
     7141 ccataaattc ataaggcagc ttgaacgttt ccgccgcttt ttcgctggga atctgtgtca
     7201 tttcaagcca ggcgatgtag aaatgttcga cggcgacgaa gaggactagg aggatgaaga
     7261 ggagtttcat gggtattcct tttaatgggt gtatttgata tggaaggtcg tctgaaaaaa
     7321 cggggaatcc gtacaggaga aaaaaccgtt ttacggtaaa ttcaaataag gacgctctaa
     7381 accgtccttt ccgcgcaggc gggaatccag agagttggaa ttgctgcaat ttttaaatat
     7441 tcctgaaaaa ccaaggtctg gattcccgcc tgcgcgggaa tgacggctta gggcaagttt
     7501 tattcaaatt cactatatcg atacgttcaa actatcccct accccccttc atactccgca
     7561 cacgatgtcg gcgtttccca caatttgacg cggcacacgt tcagccccgc gtttttcaga
     7621 cggtcgtaca tttcgaccgc catattttcg gcagtggtgc ggcagggcag gcgcagggtt
     7681 ttcatgttcc agccttccaa gagtgcggcg atttgagatt cgcgcccgtt gccgccgtgg
     7741 tagatgaagg cgtggtcgaa ggggtcggta atgtgttgtt tgacaatggc tttcaagtcg
     7801 gtaaagtcca tcaccatacc gtctttcggc ccgcctctga cggggtcgtc tgaaacggtg
     7861 atttcgagtt tgtaggtatg cccgtgcagg ttttggcatt tgccgtcatg cccgtcgagc
     7921 atatgcgagg agtcgaaggt gaagattttg gtgattttca tgggttctca aatgggcaaa
     7981 tagccttgaa agccgatttc ggcgggtttg ccgtgcggat tcggggtcag gcggatatgg
     8041 aaattgccga tgcggtcgag cgcaagcagg ttgtccacgt cttttaagtt gtctttatgt
     8101 tgctgaacat agctgtcggt caacagggtt tgcagggctt tttgtttggc ttcggcaggc
     8161 gtggcggcgg cgaacagccc aaaagcgtgc gcttcggcga gctgccctgc gcgataaccg
     8221 ccgacattgg caaaatacag gtgcggagtg tgttcagacg gcattttcgt tttgggcgtt
     8281 tcggatacgg cgatgtcgta accgtccgcc cattcgacaa cctgccagcc gtcgatgtgg
     8341 attttgtccg catcgccgaa ccacgcggct ttgagtgcgg ggacggcttc acggtagtcg
     8401 tcgcacacgg caaattggat gtcgtgcact tcgatattcg acctgccggc attgccgccg
     8461 aggtaaaaca tatggagctt gggcatatcg tattccttat ggtttgcgcg tgttcagacg
     8521 gcctttttac tttccagata ttccgccagc ccgcgttcgc gcaagataca gctcgggcat
     8581 tcgcggcagc cgccgacgat gccgttatag caggtgtggg tttgctcgcg gatatagtcc
     8641 agcacgccca tttcgtccgc caacgcccac gtttgcgcct tggtcagata catcagcggc
     8701 gtgtggattt gaaaatcata gtccatcgcc aaattaaggg taacgttcat cgatttgaca
     8761 aacacgtcgc ggcagtcggg atagccggag aagtcggttt cgcacacgcc cgcgatgatg
     8821 tgccgtatcc cctgcccttt ggcgtaaatc gcggcgtaaa gcaggaaaag cgcgttgcgg
     8881 ccgtctacaa aggtattcgg aacgccgttt tcggcagttt cgatggcggc ggtgtcgtcc
     8941 atcagggcat tgtgcgtaat ctgccgcatc aggctcaagt cgagtacggt ttgtttgacg
     9001 cccaaatcct gcgcaatcca gcgggcacgt tccagctcga cggcatggcg ttgcccgtat
     9061 tggaaagtaa tggcttggac gttttcgcgc ccgtagattt ggattgcctg aatcaggcag
     9121 gtggtcgaat cctgaccgcc cgaaaagatg accaaggctt tttggtttga catatcgaat
     9181 cctagttttg gtaacgcggg aggtttgcga accgcgaagt tggacagtaa agacgcggat
     9241 tatacaggca aacgcgttca gacggcatct gaacgccatg ccgtctgaac acgcggctta
     9301 tgccgacttg atggcgagtt ccgccaagcg tttgctttgt gcgaaggcga tattgttttc
     9361 ttcggcggtc aggacgggag ccgctgtcgg gaatatcttt tttgacggct tcgcagacgg
     9421 cggagacttt ggggacggtg cgcaatacgg cttcgcaacc ttcaacgctg tcgatgccgc
     9481 gagcgattcg gcgggcggga ttgcgggtgc tgccgttttg gaaatagtag aggacgagga
     9541 ttttgagggg gatttgggtt catgggcgga ttccgttaaa atggttcggt cttcagacgg
     9601 cataacagtt gttccgatgc cgtctgaaac agccgcagtt caggcatttg ttcctgacgg
     9661 caaaattggc tataacatcg ggcatcaatg cccaacttac tgaccgggca agaaattatg
     9721 acctttttac aacgtttgca aggtttggca gacaataaaa tctgtgcgtt tgcatggttc
     9781 gtcgtccgcc gctttgatga agaacgcgta ccgcaggcgg cggcaagcat gacgtttacg
     9841 acgctgctgg cactcgtccc cgtgctgacc gtgatggtgg cggtcgcttc gattttcccc
     9901 gtgttcgacc gctggtcgga ttcgttcgtc tccttcgtca accaaaccat tgtgccgcag
     9961 ggcgcggaca tggtgttcga ctatatcaat gcgttccgcg agcaggcgaa ccggctgacg
    10021 gcaatcggca gcatgatgct ggtcgttacc tcgctgatgc tgattcggac gatagacaat
    10081 acgttcaacc gcatctggcg ggtcaattcc cagcgtccgt ggatgatgca gtttctcgtc
    10141 tattgggctt tactgacgtt cgggccgctg tctttgggcg tgggcatttc ctttatggtc
    10201 ggctcggtac aggatgccgc gcttgcctca ggtgcgccgc agtggtcggg cgcgttgcga
    10261 acggcggcga cgctgacctt catgacgctt ttgctgtggg ggctgtaccg cttcgtgcca
    10321 aaccgcttcg ttcccgcgcg gcaggcgttt gtcggggctt tggcaacagc gttttgtctg
    10381 gaaaccgcgc gctccctctt cacttggtat atgggcaatt tcgacggcta ccgctcgatt
    10441 tacggcgcgt ttgccgccgt gccgtttttt ctgttgtggc tgaacctgtt gtggacgctg
    10501 gtcttgggcg gcgcggtgct gacttcttca ctctcctact ggcagggaga agcgttccgc
    10561 aggggcttcg actcgcgcgg acggtttgac gacgtgttga aaatcctgct gcttctggat
    10621 gcggcgcaaa aagaaggcaa agccttgcct gttcaggagt tcagacggca tatcaatatg
    10681 ggctacgacg agttgggcga gcttttggaa aagctggcgc ggcacggcta catctattcc
    10741 ggcagacagg gttgggtgtt gaaaacgggg gcggattcga ttgagttgaa cgaactcttc
    10801 aagctcttcg tttaccgtcc gttgcctgtg gaaagggatc atgtgaacca agctgtcgat
    10861 gcggtaatga caccgtgttt gcagactttg aacatgacgc tggcagagtt tgacgctcag
    10921 gcgaaaaaac ggcagtagtc ttgagtattt ttgaaactgt atttttatcc taaagacatt
    10981 ttcctatctg ctgattccac cgtttccttt ctgtgtgccg ccataatttt tatgatagac
    11041 aagccattca agtttgcatt gactgctttt tcattggcaa tatccaaatg gctggtcgct
    11101 gcatctaaaa ataggatttt cggttcgcaa tataaggccc gcgccaatac gatgcgttgt
    11161 ttttgtccgc ctgacagtgc gctgcccata tcgccgatca aggtttcata gcccataggc
    11221 atggcggaga tttcttcgtg aatcatagca aggcaggcgc aatgttcgat ttttcctcta
    11281 tccggttccg tatcgaaaaa acaaggggct gtactagatt agccctaaat cccacaccaa
    11341 tcccgcagga ttttaagctg ttgagacggt gtgccgaagt taaatcgaaa ttcgcattct
    11401 ttcaagaaca gcgggaaaga tttacgatcg attccgttgt attttcgcaa gacacgttta
    11461 gtctagagtc tgtatattac atttattttt agggtctgct agccaatttc ttgttccctt
    11521 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
    11581 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
    11641 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
    11701 taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt tcatgactaa
    11761 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
    11821 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
    11881 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
    11941 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
    12001 tttttacatt acttttttct ccccccaaag gaatgcattt taaaatcatg cttttcaggt
    12061 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
    12121 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
    12181 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
    12241 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
    12301 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa ttagaaaatc
    12361 aaaaaaaacc attaattaat ttttcttctt taaatatttt atatgatatt ataaaattaa
    12421 ataacattgc gaaaacatat gaaatcatca aaataccaaa tatatagtga gaatgcatta
    12481 ttatttagct ccttcttttg gctctgacaa attgacagtc cattgatatc caatgcttgc
    12541 atttgcaatt gctgacgcac ctgcccctgt tttccctgtt agtatcaatg gttcttttct
    12601 tgcaagatgt gcacctgcac aaccacctaa tactaaacca ccacctattg taaatccttc
    12661 ctgttgtatc tctaatgata acattttctt cttgtgccgc ccttaataca gattgtggaa
    12721 ttggattgac ctgagcagga acttctaaaa taagttttcc ttcaagctgt cttcctctta
    12781 gcagattagg tgtacttgga acattggaaa tgacaaagtg agtgcgcaaa gccatctgaa
    12841 ggcgatgtgt ttgaacctgt tgaaagcggc caacaggcta aatgcgcccg ctgccgccta
    12901 aaaggcagcc cggatgcctg atgatcgggt atccggggag gattaagggg gtatttgggt
    12961 aaaattagga gatatttggg gcgaaaacag ctgaaaacct gtgttggggt ttcggatgtc
    13021 ggggaagggc ttttttgcaa aggtctcaac tcatgttatt gcaaagatct catgccttgt
    13081 tttgccagca ggttgagttc ttctccgaca atcagcgtgg taaagccccg ccacaacaca
    13141 gatctatgta ctgtggaagg gcttcaattt atagctttcg aaaatctttc agctttaaga
    13201 cggcctaaag ttgtaatcta taaaacagga gctcagagtt ttacggctca ctctcgcttc
    13261 gcgttaccaa ataccagctt atcggttttg aacgtatagt aatctatcca atcctgcaga
    13321 tattcatacg ggtaagacaa gtcatactgt ttcataaaat cgaagatgtc ttgatattcg
    13381 gctgccggca gcggatcgta aacaatcaaa tccctcggtg cgatttcttg atctatgccc
    13441 aaatcgaaac cgtgcatgga tgcgattttg gcgtaggtaa cgatttgcgg ctgcaggtag
    13501 aaatcgaaat aggacaatgt ttcttttgca gccatacgcg cggttttttt atcgaaaagc
    13561 ggctctaagg cggctttcat tgcctcggca tcctgttcgg cgaaagcgag gaagaagcgg
    13621 taatcgtaca accgcttttg cagccatttg ctgggggtgg gatgcgccaa gactttctcg
    13681 ctacgttgtt tcaaccgatc aagctgctta ccctccacca tcagcagagt attgtaaatc
    13741 atatgacggt tgaggtcgta gcggtttata aaggcttctg tatcgttggc gatgttgtcg
    13801 atattgcgca ccagaaactc gcgcagctgc gggctgtcgc tcatcagcat cagaaacatc
    13861 ggatcttgaa tgttgagcat gtcgcaggga aagaagaagg gttcggggtc tgtagaattt
    13921 acactcagca aaactcccag ctttcctgca acataagcat attgcttgaa tttcttaaga
    13981 tcatgttcaa atagataggc atgggaagct gcagctctaa cgtccatgaa aatatgttgc
    14041 atagctgcaa gcggaacgcc ttttctttca tctacataat ctatagagtc aaggcaaccg
    14101 ctattgaaat tagcagtatt gcctatgatt acattagtaa tatgctcata ccatttttgg
    14161 gtggtcatca tattgtgccc cattgttatc tccttatatt ggctttagaa ggaactttga
    14221 caggaagaat aacggcctta cctgtttgac gatcaacgcc tgctattgct gtttttaatg
    14281 tgccgttctt atttgctttg aagacagcag ctttagcggg actaccatcg ggtaattgat
    14341 ctaaaacttg tctaatccaa tcctcactca tttgcgtata tccacccgca ccattcggat
    14401 tcagctgtac cgtaccgttc ctaatctgct tactttctac aatcaaaacg acactaccat
    14461 cggcagcctg ccatacatga tcaaaaccgt tatttccgcc gtatttgccg cccgaaagca
    14521 ctctgaagcc attttgttta gctaaagagt caaataactg ctcggtcatt ttacctgtcg
    14581 tgtcccttcc ttcgccaata gctttcagca attgtttgtc agttgctgca gtaaccctta
    14641 attcagtttg accgattttg attttggttt tactgacata gccctgtgca gtggcaacgg
    14701 ttgctgcctt gtccaaggct tttttcatgc cttgtaaatt ttttgccgct ttcgctactt
    14761 tataggcctg tatcgattca ccgatacccg gaaccacacc cagcaaagca aacaggtgat
    14821 cggcagcggt ctgtgcttgt acaaaactct gtatatcacc gataattgga ataaatcctg
    14881 tctgaagaag caaaacttct cgatattctt caggattttc acgtatccat ttttgttgtt
    14941 ccttttgaaa ttcatcacac tttgcaccaa cgcatctagg atacaaagta ttattctcca
    15001 ccgctatctg tgccgcattc gccgctgtat tcacatcccc gcctacaacg gcagacgcag
    15061 tgccggcaac cagttttgaa taggcagtaa tcttcgcttt agatttttcg atttcggtcg
    15121 cactcatacg actgaaatca gtattcttaa ccaaagcctc accaacaatc tcaccgactg
    15181 cagcgcctat cgcgccatcc tgacacttgc ccttattcgc cgccgctgcc gcacagcccg
    15241 ctatggcatg ggcaatcttg tggactatgt agtgctgatc caattgtttg attttacttg
    15301 ctgcctctcc atgcgcagta ttcaccaaag ccgcaaggat attcgcttcc agattgtctt
    15361 tcaggctgcc gccgttgaca gcggtattaa tcagtgcggc actgcccgca ttggccaggt
    15421 tgacggtcag gttgttgatc cactgcttat cgctgacatt gttcaatgcc gaagcgccga
    15481 ttttgtcggc tacgcctgcg gtagcggcgg caaccaccag atttttcacc gtgctgcttc
    15541 tgcccagctc tttcagggtg ttaccgatat tgcctttgtt gttgatgaac gatacggaag
    15601 cctggctggc caaagaggcg aatgctgcat cggttgcggc ggcggccgca ccgtttaatc
    15661 ccaatacggc tccggttcct gcgcctgagg tgaccacggt aacggccagt gcgataattg
    15721 cggctccggc tccggttagg ccttcctgtt tatagtccca tttgtcgtaa gcgagctgta
    15781 cttggttcca gttcacgtcc ttgaccgtct gaagctgttt cagataggca tattcgggct
    15841 gtttggccag cttttcgatt tcggttttga ggttgccttt ggggatgtcg gcgatatagc
    15901 cgccgggagc ggtcagctta ggcagtgccg gcccttcaaa gctcggtagc ttcagcgttt
    15961 caaccgtgct gccgcttccg gcctgctttt gccatacggt cgagttggat tccagctttt
    16021 cttcggtttg gatgcggtta acgatgcctt ttaggataat tttcgcatcg acacgggctt
    16081 tttcgcctac acctgcctga atgtcggcac cggccagcgt ggttttgaat tcggtacctt
    16141 cgagcacggt atcccagcct gaacgggtgg ctgcagtttg ggcgacgacg cggacaggca
    16201 atttggtttc gttcagttcg tttttactgt aattgctctt acctaccttg atgccgataa
    16261 agcggcggct tttttggaca tccaactcgt gcttgtgaat gccttcggct gccagcagtt
    16321 gcattgaggc cgtctgaaaa acggatattc agacggccta ttgctacttt aaataatatt
    16381 tgggtttaaa atatcatcgt atttcccaaa ttcggggaac agaaaatcaa ttatttaaat
    16441 ttttcatcct cataatatgg gtgatcaaaa agctctcctt cccacatatt tatttcttct
    16501 atgcctttat ctttcataaa ctcatgataa tcataaaaat cttttccata ttgtccgtaa
    16561 aaaattttca tatccccaag ccacatagca taacattcat cgcataaaat gacctcccca
    16621 gagaaatatt taggctttgc atgataaagg tagccttgct cacatcttgg gcaaatttta
    16681 atttctttat ccataagaga ttcctttctt aattatttct gaggatatgc agaaatgatt
    16741 ttatttgttc ctttttgcac aacaatccgc acttttgttt ctccttttgt tccaataggt
    16801 ttcttcatat ctacaagata tacggatgaa tcatttggca aaggatttcc tttcattctc
    16861 caagcttcat caaccaaagg caaaacttct ttacgggaca cattaaaaac agaatgaaca
    16921 ggtttgtttg gatttggaat tgtatgggct aaaacatgtt taatcctatt accatgctta
    16981 gcatctaacc cataatctaa ccccgccggt gttgtccaaa ccttattaac cttatcataa
    17041 gtaagcttga ttccggtctg cttctgaatt tcctgtatgg ctttagccgt atcaactgct
    17101 tcgtctcccg ccttcagaac ataagccccg cctcggctga ttttagtcgc acctgccgga
    17161 acaaagggaa cggcagctgc ggcggcatcc acaccaaaat caatcgccgc ttctcgagcc
    17221 atctgcttat cgccaataga tttggcgtaa atccatttac cgccgtccca tacgaggttg
    17281 ccgatatccc aaataatatc taatgcatta tttttaacag ctgtttcgga agcatttgcc
    17341 gccacatcaa cattgcctcc tgtaacacct acagtcgctc cggctaccaa ttttgcatac
    17401 gcagtaatat tcgctttagc tttttcaatt tcagaagcag tcataccgct gaaatcggta
    17461 ttcttaacca aagcctcccc gacaatctcc cccacgaccg caccaatcgc gccgtcctga
    17521 cacttgccct tattcgccgc cgctgccgca cagcccgcta tggcatgggc aatcttatgg
    17581 gcaatgtagt gctgatccaa ttgtttgatt ttacttgctg cctctccatg cgcagtattc
    17641 accaaagccg caaggatatt cgcttccaga ttgtctttca gactgccgcc gttaacggcg
    17701 gtgttaatca gcgcggcact gcccgcatta gccaagttga cgttgaggtt gtttacccaa
    17761 ggggtttcgc tccaagtggc aagggaggaa gcaccgattt tgttggatac gcctgcgatg
    17821 gccgctgcta caaccagttt tttcactgtg cggcttctgc ccagctcctt cagggtttta
    17881 ccgacatcgc ctttattgtt gatgagcgat acggaagcct ggctggccag cgaggcgaat
    17941 gcggcatcgg ttgccgctgc ggccgcgccg tttaagccca gtgcggctcc ggctcccgcg
    18001 cccgcagtaa ccacggtaac agccagcgca ataatcgctg ctccggctcc ggttaagcct
    18061 tcctgtttat agtcccattt gtcgtacgcc agttgtacct ggttccagtt gacgtctttg
    18121 gtgacttgga gctgtttcaa atagcaagcg tagggggtgt cttcagcacg cacgcatctg
    18181 ggcagtatat agggaatctg aatatttact tgcataacaa atgccgtctg aaaaaattga
    18241 gcttttcaga cggcattgag ccgtaaatca tggaacgcgt gcgcgctgaa gcacacacct
    18301 tacgcatgga ttttaggttt catgcaggct acagcttgct gctattcatc aaattgcggc
    18361 cattgaaagt ctgttgtttt actttcacct ctcaacagtc taatcatatc gcttttgaga
    18421 aactcaaaaa aatttttaat attaccaaca tagagcatag cttcacatag tgaactacat
    18481 gcagatttaa tgtcttcatt gtcaatagca tattgatatt ccttcatatg ctgaaaaaaa
    18541 gaatcaaagt cttcttctaa ttcatcattc caatcagatg aatagttaga aagccattgt
    18601 aagtcaagag gatcttcact attcaatttt tcagttgtgg ctttctcata aagatcaaat
    18661 ccttgtttaa ttcctaactc tcttaaactt tcttttacta cattaaaatt tttcatctga
    18721 atcaccttat ttaagattca attttcgccc ttgccctgct aatgtcttag cttaattttg
    18781 agcgagtttt aggtttcatg cagactacag cttactcagc acacacgagt ctaaacagta
    18841 tacagggaat ctaaatattt actttcataa caaatgccgt ctgaaaaaat tgagcttttc
    18901 agacggcata tggccgtaaa tcatggaacg cgtatactga agcccacacc ttatgcatgg
    18961 gttttagatt tcatgcaggc tacaacttgc tttctattca tcaagagatg gccatgaaaa
    19021 actattcttt ttatactcag cactcaataa tgttgatata tcagttttta ttgaatcaaa
    19081 tataagagat agattacctg cgtaaatcat agccataaat aaagaattac tggcaatttt
    19141 gaaattttta tcgtttaggg ctaattggca tacttccata taatctaaaa agttttttaa
    19201 atcctcctta aattcattat cccaattccc gtctgaagta taatctttaa tccatttcat
    19261 attagctgtt tcatgattaa cttcttctga tacttttgga tctgtcaaat caaacccttg
    19321 ataaagcccc acattaatca agattttttt tatatcattc aatgtttcca taaaatttcc
    19381 tattttaagt ttaatttacg accttttgct gccccagttt tcatttggtt aagcgaacca
    19441 tccatattta gaacaaactt aaagttccca tttttatcaa aaacctctaa atgattttta
    19501 tgttggccat ctaaataaaa cctatcaccg gtttttaata acccttggtt tctttttacc
    19561 aagaagacag actgcccttg ctgcgtcgga agcgttgtct tttctgaaat ttgagccagc
    19621 tgttttccaa aaggattatt tttcatgtat gtactcatat tcggtacagc acctttatta
    19681 gggatataag gacgattttt ttctaaaact tccttgacct tttgtgccgc ttccccttta
    19741 ttagcgcgat tcagctctgt tccgacgaca atatcaataa cggctttggc atcgttccaa
    19801 tccaatgttt cgtcgaataa ggtggtcagg ttgtcggcta aattataacc ttcgtctttc
    19861 aacgtctgtt ttaaatctct aacgttgatt ttcccgtttt ttaatccttt tctggctacc
    19921 ttataaacca cttttgcagc agttacaaca gctttaaccg cattattttc taccgcgttt
    19981 tgtgcggttt gtgcagcagt attgacatct cctcccgtta cgcctgcaac tgtacctgcc
    20041 gcaagtttgg cataggcggt aattttctta acttccagat ctaattgttc cggggtcata
    20101 tcgctaaaat cggtattttt aaccaaagct tccccaacaa tctcacccac ggctgcgcca
    20161 atcgcgccgt cccgacattt gcctttattt gctaccgccg atgcacagcc tgctacggca
    20221 tgggctatct tgtgggcaat gtagtcttcg ctgagattaa atttgatttt gctcgctact
    20281 tctccgtgta cggtactgac tatcgcaccc agtgcggcat cgcccaagtt gtctttcagg
    20341 ctgccgccgt tgatggcggt atggacactt gcggcagcgg tgctgttgat caggttagcg
    20401 gtcagtttgc ctgctgcggg actgtgaaaa tgtttgctga cggcttcggc tgcttgggtg
    20461 ttcagcccgc ttatgccctg cagtacgcct gcggttacgg cggcggtggc ggcctgtctg
    20521 acggtgctgc ttttgcccag ttctttcagg gtatggttta tgtctccttt gttgttgatg
    20581 agggaaactg cggcttggct ggcgagactg gccagtgcgg ctttgcctgc ggctgtggtg
    20641 atagctgcag ctgatgtgcc tgctgctact ccggttgtgg ctgccgttcc tgctgctact
    20701 ccagttccgg ctgccgttcc tgctgcggct cctcccgctc ctgcgcctgc cgcgcccgcc
    20761 gttccggcgg ctgccggggc ggacagtgca ccgtaggtca atacggttac gacgataacg
    20821 acgacagctg ctgctgcggg tgtcatgccc tcctgtttgt agtcccattt atcgtaagca
    20881 agctgcacct gattccagtt gatgtttttc gcaacttgaa gttgtttcaa ataagcatac
    20941 tcgggctgtt tggcgagttt ttcgatttcg gttttgaggt tgcctttggg gatgtcggcg
    21001 atatagccgc cgggagcggt cagtacgggc gcaacgggac cggtgaaact cggcaattgc
    21061 aaggtttcga tgttactgcc ccatcctgcc tgtttttgcc atagagtaga tttgctgctg
    21121 ctcacggttt ctgtgtggat gctgcttttg atcccttcga ggataatctt ggcatctgcc
    21181 cgtgcctgct cgcctacgcc tgcgcgtatg gttgcgccac ccagtgtggt ttcaaactgt
    21241 gtgccttgca gtttggtatc ccaacctgat tgcagattgg cagattctgc aactaccctt
    21301 gagggcagcg cggttttcat gacttgggtg gtggtgtcgt gtgctttgct gtagctgatg
    21361 ccaagaaatc tgcgcctttt ttggctgtca agtttgtcgt agttgagctc ttctacggca
    21421 tagagtgtca atttccgccc ggcttcgatg ttaatgctgc ctttgggggc atcgaatgcg
    21481 gtggcgtagg cgtcgatgtt gccaccagat ttgatgtcga tgccttggga tgcgctgagg
    21541 tttactgcgt cgggcttggc gtttttgtgt tctttgactt cggtaatgtg tttgcggttg
    21601 taccatttgc cggttttgta gctgcggcgt tcaaaggtat agagttcgct ctgtctcgca
    21661 tagtaatatt ggtcaccgta agattgaatt ttgattttgc cgttttccga actgatatcc
    21721 gtggtgctga gcaggatgcg actgttctca ttggcatacg gtgcagagag ggtaacacct
    21781 gtttttcctg aaatttcaga tggcctgctg aaaatggcat agccgtattt atcgctgcct
    21841 tgctgaccat attcaaaagt atcaaataca ccgctgatgt caatggctga ttgaagtcga
    21901 ccttcttgca gttcctctgc ggctggtgca ggtaaaaatc ctgcagcttg gatatttgcc
    21961 tgttgggtgg cagcaacttt ggagccctgg atacggatac cacctgcagc gagcagtttg
    22021 atattttgac cgctaagggt gctaccttgg ttttcgtgac cactacccgg ttgggaaact
    22081 gttaaaaggg cttgttgtaa caattgttta cgttcaagta acgcttttac tggggattgg
    22141 agttctgccc aagcctcatc agcagcattt atctctgctc gtaaaccacg taatctggcc
    22201 tcactatcac cgaatacttt gtcacgcctc ttatatttac gcctcaacat ttgtggaaga
    22261 tcctgtgctt tacgatattt aggatcgctc gttaacttac taagctcctg ctctacatca
    22321 gtaagttgct gtgtgatatt gtgtttatct gttttcaatt gatagttact aaacgtattc
    22381 ctaatactgt ttagtgaaat attcccttta gttgccacta aagccaaatc tttccctgcg
    22441 gccatatttg ctgcatcaat atccacctct cccgccacaa tcccgatatt gcctttagct
    22501 gaaacgctgg ctgtttgcgc actcggattg ccgttatttc ctgctctggc actgatttgg
    22561 aatgtgccgt tgtggttcac ggccaaatca cctgcagaaa cgatacggtt accgggttca
    22621 acggttaaag ggttattacc gcccgattct atcgacatca taccggacag tggtttcagt
    22681 tccgctgaac gcatagttgc cgtatcttgg gtttcccaat cgatatgccc ccgtttgatg
    22741 atgcctccgc cagctttgat gtttatcgcg ccaccgcgca gggtagtatt gtccaaaacc
    22801 tgtaatgcgc tgctgttcaa tgacaataca ccatctgccg ccaaagtact tttactgttt
    22861 tgctggtcgt tttgtaaaat ttgaccgctg tcttttcctt tgcctttacc ctgtgcactg
    22921 atactcatat gacgacgtga atgagcatca agctgattaa gcgtcatatg accatgattt
    22981 gtattaatct gggtattgtg accggaattg agcttgctgt tattaatggt taaagtttga
    23041 tccgcatcca ctttcagtga tgcgcgggca ttcaggtgta catcctgcac caccatgctg
    23101 cctttattgc tgtctaagct gatatgaccg ccgttaacgg tgccgttgcc taaatctaat
    23161 tgacctccgg caaccagttt cacatcgcct gcagaagcat aaatatcggt attgtccatt
    23221 ttcagacggc ctttaccgac tgaaccagca ttaatgtctc cttccgcaat caaggtattt
    23281 agaccgttga aatcaacatt gccgttggca agaagagata cgcgtccaac ttcggcaaca
    23341 gcagtcagac cgtctgaaat aatattgcct tgaagtgctg ccgtatcaat attctgactg
    23401 gcatttatcc aactctgatt caagctgatg ttgcctttag tcgcgctcat ctgcacatta
    23461 ccgccactgg attgcagatt actttgacgc acactcaagc tacctgcctg aatatccaga
    23521 ttcttttctg cagtcaaggt gttaccgttg ctactgatga gggcggtgtt gtctgccctc
    23581 aggctgatac tttgtgttga taagtcttta tcggcattca aatccagatc cttacctgca
    23641 tgaatataca gattgccgga tgcattcaga ttggaggctt ttgcctgaat gtcgccatca
    23701 gccatcagta ccacctgctt tccgccttta atgttaccgt tgttcaaacg gatgttggag
    23761 gcaacggtcg aggtttctaa agaaagcggt ttgcccgatt caatgtgtgc agtgtcttga
    23821 gcctcaatta cagcagcact gctaatacta ccgttagata atacggttac gttttcagca
    23881 ataatacggg tattttcacc caattcagta tcgcctttgg tggagctgta cacggaactg
    23941 cccgcttgaa tagtagcatc attgatcgta gtacgaccgc cggccgacag attagccgag
    24001 cctttggcat tgttcacatt agttttactc tcaatcacca aattatgacc agcatttaat
    24061 accgtggtag ctgggcgact gccgttattc tgcaccacgg ctccgttacg caagctgata
    24121 tcttctcccg tctcaataac caataagcct ttgctctcga tccgaccacc attggagata
    24181 aatgtgcctg ccgctccttt ttcggtggtt tcgatggaga gataagtcgg tgaagcttcg
    24241 gtgccgtcgg cagtggtggc gatgcggccg ctgttttcaa tgcggcctga cgaagtcaca
    24301 atcaattgct tggccgcttc gagtgtgccg gcatttttga cgcctacgcc tttttcattg
    24361 gcaatcagtg tgatgctgtc ggcgtacata ccgcccagtg cggcagtatc aagggcaata
    24421 gtcggtttcg tacccgctgc cgtacctgca ctgatttcgc cgctggcgta atctactttc
    24481 tgaggaccgg tagaaaccgc caggttttta ccctgtaatt tcccctgcaa agcaactgca
    24541 cgagcaagta tctcggtgta gtcggctccg cctttatcat tccaacctgc tgctcctacg
    24601 gtcaatgtgc cttgacgcac atcaaatcct gtcagtgcac cgtctttgcc gatttggggc
    24661 gcaccggtag ttaagatgcc ccgaccgaca tttttaaagc cgccgccatt aacggtaatg
    24721 ccgttggggt tggcaataat cacgtcggcc ttttggccgc ctacggtaac gatgccgttg
    24781 agtttgctag ccgtaccgcg tacctcgttc aaaatcaatt gcgcaccgcc tttgaccaga
    24841 aacggattat tgttacggtc gttgtttaac actgcccctt tgttgtcaac atcaaactgc
    24901 gtatagcggt tgtggctcaa tccgcgtcca ttcggagttt ggatattcac caagggggca
    24961 ccagtgttgg ttttaaggat aacgacctgc tggtttttag gtgctgattt gtcggtggta
    25021 atttgggcat gggcaggcaa taccatactc agggaaacca aagagcagac caaggtttta
    25081 agggtggttt tgagtttgcc gcaaaggtcg cctgaagttt tcagtgaaac agaaaccgaa
    25141 ctgcctgcct gtttaccttt gccctggctg ttggcagttt cggctactgc aaccatggtg
    25201 ctgtgctttt
//
LOCUS       DAWFBO010000008        24708 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFBO010000008 DAWFBO010000000
VERSION     DAWFBO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.008"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            167..790
                     /gene="lolA"
                     /locus_tag="WGO87_000240"
     CDS             167..790
                     /gene="lolA"
                     /locus_tag="WGO87_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0391668.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPALFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1063..2202
                     /locus_tag="WGO87_000241"
     CDS             1063..2202
                     /locus_tag="WGO87_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0391669.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAHPPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2335..3391
                     /locus_tag="WGO87_000242"
                     /pseudo
     CDS             2335..3391
                     /locus_tag="WGO87_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            3432..3962
                     /locus_tag="WGO87_000243"
     CDS             3432..3962
                     /locus_tag="WGO87_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0391670.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(3979..4110)
                     /locus_tag="WGO87_000244"
     CDS             complement(3979..4110)
                     /locus_tag="WGO87_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ0391671.1"
                     /translation="MPSEPEKRLSDGIFACNSNAAGQNQGASDTVIGHPVRMNLGLT"
     gene            complement(4119..5714)
                     /locus_tag="WGO87_000245"
     CDS             complement(4119..5714)
                     /locus_tag="WGO87_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0391672.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCEDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(5821..6216)
                     /gene="hisI"
                     /locus_tag="WGO87_000246"
     CDS             complement(5821..6216)
                     /gene="hisI"
                     /locus_tag="WGO87_000246"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0391673.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(6247..7014)
                     /gene="hisF"
                     /locus_tag="WGO87_000247"
     CDS             complement(6247..7014)
                     /gene="hisF"
                     /locus_tag="WGO87_000247"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0391674.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPGNTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(7027..7764)
                     /gene="hisA"
                     /locus_tag="WGO87_000248"
     CDS             complement(7027..7764)
                     /gene="hisA"
                     /locus_tag="WGO87_000248"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0391675.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(7797..8435)
                     /gene="hisH"
                     /locus_tag="WGO87_000249"
     CDS             complement(7797..8435)
                     /gene="hisH"
                     /locus_tag="WGO87_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0391676.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(8565..10067)
                     /gene="pta"
                     /locus_tag="WGO87_000250"
     CDS             complement(8565..10067)
                     /gene="pta"
                     /locus_tag="WGO87_000250"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0391677.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGFAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(10252..11310)
                     /gene="fbpC"
                     /locus_tag="WGO87_000251"
     CDS             complement(10252..11310)
                     /gene="fbpC"
                     /locus_tag="WGO87_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0391678.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSAAPAGTRGTL
                     LIRPEQFSLYPHSAPAAAIHAVVLKTTPKARHTEISLRAGQTVLTLNLPAAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(11331..12848)
                     /gene="fbpB"
                     /locus_tag="WGO87_000252"
     CDS             complement(11331..12848)
                     /gene="fbpB"
                     /locus_tag="WGO87_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0391679.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(12916..13911)
                     /gene="fbpA"
                     /locus_tag="WGO87_000253"
     CDS             complement(12916..13911)
                     /gene="fbpA"
                     /locus_tag="WGO87_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0391680.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(14163..14633)
                     /locus_tag="WGO87_000254"
     CDS             complement(14163..14633)
                     /locus_tag="WGO87_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ0391681.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGWEQELVLIW
                     RAEADIGNGGILQFVCNWSFPAAEKTCSVLKKIGAIHSAMLIHRAADTLDKEIRHLQS
                     EGKNLKEMWDITQHLDNKTVSLLNSLDEQYWQDPDKLYLLGWQYYSGNPVQTAF"
     gene            complement(14690..16066)
                     /gene="argH"
                     /locus_tag="WGO87_000255"
     CDS             complement(14690..16066)
                     /gene="argH"
                     /locus_tag="WGO87_000255"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0391682.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAKLLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSKLPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMSA"
     gene            complement(16085..16954)
                     /gene="galU"
                     /locus_tag="WGO87_000256"
     CDS             complement(16085..16954)
                     /gene="galU"
                     /locus_tag="WGO87_000256"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0391683.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(16982..17581)
                     /gene="rdgB"
                     /locus_tag="WGO87_000257"
     CDS             complement(16982..17581)
                     /gene="rdgB"
                     /locus_tag="WGO87_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0391684.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(17574..17807)
                     /locus_tag="WGO87_000258"
     CDS             complement(17574..17807)
                     /locus_tag="WGO87_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391685.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(17915..18448)
                     /locus_tag="WGO87_000259"
     CDS             complement(17915..18448)
                     /locus_tag="WGO87_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0391686.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(18550..19008)
                     /gene="nudB"
                     /locus_tag="WGO87_000260"
     CDS             complement(18550..19008)
                     /gene="nudB"
                     /locus_tag="WGO87_000260"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0391687.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            19219..19295
                     /locus_tag="WGO87_000261"
     tRNA            19219..19295
                     /locus_tag="WGO87_000261"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19253..19255,aa:Pro,seq:ggg)
     gene            19453..21852
                     /gene="mafB"
                     /locus_tag="WGO87_000262"
     CDS             19453..21852
                     /gene="mafB"
                     /locus_tag="WGO87_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0391688.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLVDRFHNTGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKDVKYDTRINTAVPQVNPIDEPVF
                     NPKGSVGSAHSWSITARIQYAKLPRQGRIRYIPPKNYSPSAPLPKGPNNGYSAGNLSK
                     KVLTLVYINGKLKFGPSSLPSSALKAPVPIVGKAEAPPGKAVPVGEARIPSNPKAVKG
                     TTVTLSQEQRLANGELIPKGSVVTWSDNAVKVVRPDGKKRRFNSRDWEQKALPKPQLP
                     KDYRDDINIGGNNPRFNQQRHEKIESHNPNRIEEIRQRNAVSSAERQADALKRMQTSG
                     RTRLPGQPQKGKPGAAREMSSSDNPNQTAEAFARRYLGRKPTLMERVEGEKLYGGKCV
                     GCWKAQMPDGTFVVYRPAGNASMTKGTTASVDINNPTLNNLNINLKGKPTELKFKFPA
                     R"
     gene            21863..22324
                     /locus_tag="WGO87_000263"
     CDS             21863..22324
                     /locus_tag="WGO87_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391689.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            22350..22598
                     /locus_tag="WGO87_000264"
     CDS             22350..22598
                     /locus_tag="WGO87_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0391690.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            22625..23044
                     /locus_tag="WGO87_000265"
     CDS             22625..23044
                     /locus_tag="WGO87_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391691.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            23122..23205
                     /locus_tag="WGO87_000266"
                     /pseudo
     CDS             23122..23205
                     /locus_tag="WGO87_000266"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23202..23444
                     /locus_tag="WGO87_000267"
     CDS             23202..23444
                     /locus_tag="WGO87_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0391692.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            23522..23767
                     /locus_tag="WGO87_000268"
     CDS             23522..23767
                     /locus_tag="WGO87_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0391693.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGKDKI
                     PSYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            23770..24159
                     /locus_tag="WGO87_000269"
     CDS             23770..24159
                     /locus_tag="WGO87_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391694.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            24377..24649
                     /locus_tag="WGO87_000270"
     CDS             24377..24649
                     /locus_tag="WGO87_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391695.1"
                     /translation="MPIDNIPFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALT
                     GIAFLRGIDVYDPPVSKEKALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         5931 a         5831 c         6911 g         6035 t
ORIGIN      
        1 cggcacacat taaagggcag cgtggcgcgc ctgccttttc cggtgggcaa aaaatcggcc
       61 ctcggaaaac gctgtttgta aaatgcaaac cgcccgtaac gccgcccgta tgattgtttt
      121 gccgcgccga tactttacgc cacacccatc ccgacaagga aaaataatga tgaaaccgca
      181 caacctgttc caattcctcg ccgtttgctc cctgaccgtc tccgtcgctt ccgcacaggc
      241 gggcgcggtg gacgcgctca agcaattcaa caacgatgcc gacggtatca gcggcagctt
      301 cacccaaacc gtccaaagca aaaagaaaac ccaaaccgcg cacggcacgt tcaaaatcct
      361 gcgcccggcc ctcttcaaat gggaatacac ttcgccctac aaacagacta ttgtcggcga
      421 cggtcaaacc gtttggctct acgatgtcga tttggcacaa gtgaccaagt cgtcccaaga
      481 ccaggccata ggcggcagcc ccgccgccat cctgtcgaac aaaaccgccc tcgaaagcag
      541 ctacacgctg aaagaggacg gttcgtccaa cggcatcgat tatgtgctgg caacgcccaa
      601 acgcaacaat gccggctacc aatacatccg catcggcttc aaaggcggca acctcgccgc
      661 catgcagctt aaagacagct tcggcaacca aacctccatc agtttcggcg gtttgaatac
      721 caatccccaa ctctcgcgcg gcgcgttcaa gtttaccccg cccaaaggcg tggacgtgtt
      781 gagcaactga tgccgtccgc cccgatgccg tctgaaagcc gccgaggctt cagacggcat
      841 tttcacgcag gcggaacaat gtcccgcatt accgcccgat cgggcatcgg aacggcaaac
      901 ccgtgaaaat taacggttgc gccccggttg tttttgccgt ttaatgcaaa ccttgctgca
      961 ccaagggcca agaaagccga ccggccgccc ccacagcttc cgatgcaggc ggcccggccg
     1021 tccctgcaat gttttttatt tttgaacgaa aggtcgaaaa ccatgaaaaa aacactggtg
     1081 gcggcggcaa tcctgagcct cgccttgact gcgtgcggcg gcggaagcga taccgccgcc
     1141 caccccccct ccgccaagcc cgaagccgaa caatcgggca aactcaatat ctacaactgg
     1201 tcggattatg tcgatcccga aaccgtcgcc gcctttgaaa aagaaaccgg catcaagacg
     1261 cgttccgatt attacgacag caacgaaaca ctggaggcaa aagtcctgac cggcaaatcc
     1321 ggctacgacc tgaccgcgcc gtccatcgcc aacgtcggcc ggcaaatcaa agcgggcgcg
     1381 tatcagaaaa tcgacaaggc gcaaatcccc cattacggca acatcgataa agatttgctg
     1441 aaaatgatgg aagccgtcga tccgggcaac gaatacgccg tcccctattt ctggggcatc
     1501 aacaccttgg caatcaacac gcggcaggtg caaaaggcat tgggtacgga caagctgccc
     1561 gaaaacgaat gggatttggt gttcaacccc gaatacaccg ccaaactcaa atcctgcggc
     1621 atcagctatt tcgacagcgc aatcgaacag attcccttgg cgttgcacta tttgggcaaa
     1681 gaccccaaca gtgaaaatcc cgaagacatc aaagccgccg tcgatatgat gaaagccgtc
     1741 cgaggcgacg tgaaacgctt cagctcttcc ggctatatcg acgatatggc ggcgggcaac
     1801 ctgtgtgccg ccatcggtta cggcggcgat ttgaacattg ccaaaacccg tgccgaagaa
     1861 gccgcaaacg gcgtggaaat caaagtattg accccgaaaa ccggcgtggg cgtgtgggtg
     1921 gattccttta tgattccgcg cgacgcgcaa aacgtcgcca acgcccaccg ctatatcgac
     1981 tacacgctcc ggcccgaggt ggcggcgaaa aacggcagct tcgttaccta cgcgcccgcc
     2041 agccgtcccg cgcgcgagct gatggatgaa aaatacacct ccgacgcatc gattttcccg
     2101 aacaaagaac tgatggaaaa aagtttcatc gtatcgccca aatccgcaga atccgccaaa
     2161 ctgggcgtga agctgtggca agggctcaag gccggaaaat aaccgcaacc cctgccgtct
     2221 gaaacctttc gggcggcagg aaacggcgcg tccgttatca aacagggggg cgtttcccct
     2281 cctgccggtt atgattgggt taagattaaa atgatttagt aaaatgagaa agatatggat
     2341 ttaagtatcg tagttcccat ttataatgtc gaaagttatt tggaagcgtg tttaagttcc
     2401 atagaaccta tattaagtaa tgaaaatgtc gaacttatcc ttgtgaatga cgggtcaaaa
     2461 gacggaagtg aagatatatg ttataaatat atagataaaa caacaaacaa caaacaacaa
     2521 acaacaaaca acaaacaaca aacaacaaac aacaaacaac aaacaacaaa caacaaacaa
     2581 caccagatac cgaacaccaa acatcaaata tatatatcag gataaccaag ggacggcaga
     2641 agctaggaat accggaataa aaaattcaaa cggaaaatat atagtcttta ttgattcaga
     2701 tgattttatt aattgtcagg tcttgctgga ttttcttagt aaagatgata ttgatatgcc
     2761 ggatgtggtt tttttaaatg cggttaaata tgataaggga agtgtttcac attttggcga
     2821 agattatcag cctgaaaaaa tactcaagca atccaaagtc gaagttttga aaggattatg
     2881 ccgatttaga aaatttccgg gttcggcgtg taataagatt ataaaaaggg aattgattat
     2941 caaagaaaaa ctgttttttg aaaaagaggt ttatgccgaa gatatcgaat ggtcaatgag
     3001 attatttaat gcggcaacaa ctttttctta tttggacggt tgttattact attatcggca
     3061 gggaagaaaa gattctaaaa caggaactgt ttcggaaaaa agtataaagt cattattata
     3121 tattttggag aaaaatgcgg aaatggaatt tgatagggat atatcaagtt atctttattc
     3181 ttttctttcc tacgaatatc tcgttttgct ttttatcatg acgagtaaaa atatagagtg
     3241 tgatgctgat ataaaaagaa gggcgtatca tttaaggttt atgctgttaa agtccaataa
     3301 attgatatat aagctgatat ttccgataat cacattattc ggggttgata ttacaggcag
     3361 gattttaaaa gcaatcaggg ggaatattta ataaatcctt taacactata catcttaccg
     3421 aaggaggaaa aatgaacgca atccgaactt tccaaaaccg cacgcccgaa atccacgaaa
     3481 gctgtatgat agacgaagcg tgcgtcgtca tcggcgaagt gtcgcttgcc gaagacgttt
     3541 ctgtgtggcc gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca
     3601 gcaatataca ggacggcagc gtcctgcacg tttcccacaa aaccgccgcc aaacccgaag
     3661 ggtcgccgct ggttatcggc gaagacgtta ccgtggggca caaagtgatg ctgcacggct
     3721 gccgtatcgg caaccgcgtc ctggtcggca tggggacgac ggttctggac gatgccgtga
     3781 ttgaggacga agtgatgatc ggcgcgggca gcctcgtgcc tccgcgcaaa cgtttggagg
     3841 gcggctatct ttatgtcggt tcgccggtca gacaagtgcg cgtgctgacc gatgaggaaa
     3901 aagccttttt gaaatattcc gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt
     3961 gaaatcacat cggcgttcct acgtcagccc caaattcatg cggacgggat gcccgataac
     4021 ggtatccgat gcgccttgat tttgaccggc tgcgtttgag ttgcaggcaa aaatgccgtc
     4081 tgaaagcctt ttttcgggtt cagacggcat tttatcgcct aaagtttgac cgaatgttcg
     4141 cgcgtttcgt ggaacacgat gtctggccaa cgttcttgcg tcaaccccaa attcacgcgg
     4201 ttgggggcga ggtaggcgag gttgccgccc gcgtcgatgg cgaggttgcc cgcgttggct
     4261 ttttcaaatt cagccagctt tttcttgtct tcgcacgata cccagcgcgc cgaccagatg
     4321 gatgcgctgt cgaacacggc ttctacgccg tattcgttgg cgaggcgcga ggttacgact
     4381 tcaaactgca acacgccgac cgcacccaaa atcaaatccg cgccgctcat cggtttgaac
     4441 acctgtaccg caccttcttc gccaagctgt tgcaaacctt tttgcagttg cttgattttc
     4501 agcgggtttt tgatgcgaac gctgcggaac agttcgggcg cgaagaatgg gatgccggta
     4561 aacgtcagtt gttcgccttc ggagaagctg tcgccgattt ggatgttgcc gtggtttggg
     4621 ataccgataa tgtcgccggc gtaggcttct tcaaccagct cgcggtcgtg ggacatgaag
     4681 gttaccacgc tggaggcggc gatttcgcgg ttgatacgca ggtgtttcat tttcatgccg
     4741 cgctcgaatt tgccggagca gacgcgcaag aaggcaatac ggtcgcggtg tttcgggtcc
     4801 atattggctt ggattttgaa gataaatccg gaaaacttcg gctcgtccgg ctcgaccata
     4861 cgcacggtcg catcgcgtgg tttcggcgcg ggcgcccatt caatcaatga attgaggatt
     4921 tcctgaatac cgaagttgtt aatcgcagag ccgaagaata cgggcgtgag ttcgccggcg
     4981 aggaattcgt cgagattgaa ctcgttggaa gccgcctgca ccaattcgat ttcgtcgcgc
     5041 aactgttgga tttccaatgg aaagcgttgt tccaattcgg gattttctat gcctttgatg
     5101 atgtcgaact cgtgcggcag gcgttcgccg cccgcttcaa agagatagat ttcgtcgttc
     5161 aggatgtggt atacgccttt gaagtttttg cccataccga tcggccaggt aacgggcgcg
     5221 cagcggattt gcaggatgtt ttccacttcg tccaagagtt ccagcgaatc gcgcacttcg
     5281 cggtcgtatt tgttcataaa cgtaacaatc ggcgtatcgc gcaggcggca gacgtttaag
     5341 agcttgatgg tttgcgcttc cacgcctttt gccgcgtcga tgaccattaa tgcgctgtcc
     5401 acggcggtta aaacgcggta ggtgtcttcg gagaagtctt ggtgtcccgg cgtgtccaag
     5461 aggttgacgg tgtggtcttt gtagtcgaac tgcatcacgc ttgatgccac ggaaatgccg
     5521 cgctgcttct cgatttccat ccagtcggaa gtggcgaatt tgccggtttt tttgcctttt
     5581 accgtacccg cgctttgaat cgcacctgaa aacagcaaga gtttttcagt caacgtggtt
     5641 ttacctgcgt cagggtggga gatgatggca aacgtgcggc ggcggcgcac ttggtcgagg
     5701 atttcttggg acatggtttt ctttgcaaaa aggttcaggc cgcttttcag acggcccgga
     5761 cagtgtttga gacggcgaaa ttgtacaaaa aaatgcctga taattcaatg ttggaggcgg
     5821 tcagtgcgtg ctgccgtaaa tctctttttc gtctttcagg acggcatcgg cggtttccca
     5881 cgcgctgcca cgccagactt tgtaaaagca gctttctcgc ccggtgtggc aggcgatgcc
     5941 gccgttttgg gcgatgagca tcacaatggc gtcgccgtcg cagtcgaggc gcagttcgcg
     6001 gactttttgc gtgtgtcccg actcttcgcc cttcatccat tgtttttggc gcgaacggct
     6061 gtaatagtgg gcaaagccgg tttcgacggt tttttgcagg gcttcggcgt tcatccacgc
     6121 caccattaaa acacgtttgg tttcggcatc ttgggcgatg gcgcaaacca aacctttttc
     6181 gtcaaatttg acggcttcaa gcaggttttt atccatattt tctttcagac ggcatggtcg
     6241 gggcggtcag aggcgcactt cgatgccggc ttcgcgcata gcgcgtttgg cttcgcggat
     6301 ggcgatttcc ccgaaatgga aaacgccggc ggcaagtacg gcatcggctt tgccttcggt
     6361 tatgccttca atcaggtgcc ggacattgcc gaccccgccg gaggcgatga cggggatgtc
     6421 gacggcttcg gcaacggcgc gggtcagcgg caggttgaaa ccctgtttcg taccgtccct
     6481 gtccataccg gtaagcagga tttcgcccgc gccgcgtttt tgcatttcga tcgcccattc
     6541 caccgcatcc aaaccggtcg gattgcgccc gccgtgggta aagatttccc agcgtgtgtt
     6601 tccggggttg acggctttgg catcgacggc ggcgacgatg gcttgcgaac cgaaaaatcc
     6661 ggcggcttcg tcaatcaggt cgggacgggt aacggcggcg gtgttgatgc taactttgtc
     6721 cgcgccggca ttgagcaggc ggcggatgtc ggcaacggtg cgtacgccgc cgccgacggt
     6781 cagggggatg aagacttgtc cggcaacttc ttcgatgatg tgcaggatgg tgtcgcggtt
     6841 gtcggatgag gcggtgatgt cgaggaaggt caattcgtcc gcgccttcgc cgttgtagcg
     6901 tttggcggct tcgacggggt cgcccgcgtc gcgcaaaccg atgaagttca cgcctttgac
     6961 gacgcgcccg tcttttacgt cgagacaggg gatgatgcgt tttgccagtg ccataatcgg
     7021 atgcctttag tcgagggaat ctgccagttg ctgcgcttgg gcaaaatcga tgctgccctc
     7081 gtaaatcgcg cggccggtaa tcgcgcctgc tacgccatgt ttttcggcgg cacacagggc
     7141 gcggatgtcg tccaagccgg tcagtccgcc ggaggcgatg acgggaatgc ggacggcttg
     7201 ggcgagtttg accgtcgcgt cgatgttcac gccgctcatc ataccgtcgc gcccgatgtc
     7261 ggtgtagatg atgctgttga cgccgtcgtc ttcaaagcgt tttgccaaat caattacatg
     7321 atgcccggtt acggttgccc agccgtcgat ggcggccata ccgtctttgg catccagccc
     7381 gacaataatc ctgccgggga aggctttgca cgcctcgcgc acgaagtcgg ggtttttgac
     7441 cgccgccgtg ccgataatca cgtcgtttaa gcccaaatcc aaatattgtc cgatggtttt
     7501 caaatcgcgt atgccgccgc cgagctgtac ggggatgtct ttggcgacag cggcaaggat
     7561 gtctttgatg gcgggcaaat tttgcggcac gccggcaaac gcgccgttca aatctaccag
     7621 atgcaggcgg cgcgcgcctt gtttgaacca gtgcagcgcg gtttcggcgg gcgaatcgga
     7681 aaagacggtc gcctcttcca tcagcccttg tttcaggcgg acgcagcgtc cttctttcaa
     7741 atcgatggcg ggtatcagca gcatagtttt tctccttgtg cggggccgtg tccggcttac
     7801 cagtttaaaa agtttttcaa catcgtcagc ccggcatcgt ggcttttttc ggtgtgaaat
     7861 tgcgtggcga atacgttgtc tttgccgacg atgcaggcaa acggggacgg gtagtcgctt
     7921 tcgcccaata tggtttcggg attttcgggg gcgaaatagt agctgtggac gaagtaaaaa
     7981 cgcgtgtctt ggggaatatc tttaaacagc gggtggtttt gggtttggcg cacggtgttc
     8041 cagcccatat gcgggacttt cagacggcat ccctgcgggt cgcggaggtc gcgctcaaag
     8101 cgtctgactt tgccgccgaa ccagcccaag ccgtcggtgt ttccttcttc gctgtggtcg
     8161 aataaaagtt gcgcgccgac gcagattccg aaaaagggtt tgttttttaa ggcatctttg
     8221 actgcctcgt ccaggccgcc gcgcgtcagt gccgccatac agtcgggcat cgcgccctgc
     8281 ccgggaaaaa tgactttgtc ggcgcgggac acgcgttcgg ggtcgccgct taaaaagatt
     8341 tcggtatttt ttccggcaag ctgccccgcc gtccggacgg atttcaatac ggaatgcagg
     8401 ttgcccatac cgtaatcgat aatggcggtt tgcatggctt cctcctcttt tttgcaatat
     8461 ggctgcgatt ttaacaaaca aatgtgccgt gctgataaaa atgccgtctg aaaacgggag
     8521 tctgtcttca gacggcatag ggtttaaacc cggaaagccg tttgtcagcc ttccatttgt
     8581 tttgcctgaa cggcagtcag ggcgatggtg aacacgatgt cttccaccag cgcgccgcgc
     8641 gacaggtcgt tgaccggttt gcgcaggcct tgcagcagcg ggccgacgct taagacgttg
     8701 gcactgcgtt ggacggcttt ataggtgcag ttgccggtgt tcaggtcggg gaagaccaaa
     8761 acggttgcct gtcctgccac cgggctgccc ggagctttgg atttgcccac gcccggcacg
     8821 gttgccgcat catattgcag cgggccgtcg atggcgaggt cggggcgttt ttcccgggca
     8881 agtttggttg cttcgatgac ggtatcgaca tcggggccgc tgccggagtt gacggtggag
     8941 taggaaatca tcgccacttt cgggtcgatg ccgaaggctt ttgcggaatc ggcagactgg
     9001 atggcgatgt cggcaagctg ttgcgcggtc gggttcggat taaccgcgca gtcgccgaag
     9061 acgaggactt ggttgggcag cagcataaag aatacgctgg acacgaggct tgcgcccggt
     9121 gcggttttaa tcagttgcaa agcggggcgg atggtgttgg cggtggtgtg aaccgcaccg
     9181 gataccaaac cgtccacatc attttgcgcc atcatcatcg taccgagtac cacggtgtct
     9241 tgcagttgct tgcgcgcgtc ttcgggtgtc aggcctttgg atttgcgcag ttcgcacatc
     9301 ggtccgacgt attgttcgac caatgaggcg ggatcgatga tttccaaaga gtcgggcagg
     9361 ctgataccgc gttctttggc aacggcttcg acttcttcgc gtttggcaag caggacgcag
     9421 cgggcaatgc ctttttcgtg gcagatggcg gcggcttgga cggtgcgggg ttctgcgcct
     9481 tcaggcagga cgatgcgttt gtcggcttgg cgggcgaagt cgatcaggtt gtagcggaat
     9541 tgcgccggcg acaggcgttt tgcttcgcgg cctgccaata cggatacgtc tttcagcgcg
     9601 tcgctcgaac cgaagaaggt caggccggtt ttttcggctg ccgcttcggc aacggaggct
     9661 gccgcgccgt ccacgacaaa accttccaat acgcccggcg cggcggcgaa gaactgtttg
     9721 gcaaggttca cccgatttgc cagttcgtcg gcatcggcgt tgtcggaacg gacggcgaag
     9781 acggctgccg cgtcaaggga caatgccagt tcgacgtttt tgcctgcgag gtagattttg
     9841 tcggcatcgg gcgcgaagcc ttcgatgacg aggttggcag catcgagtgc ggcaactttg
     9901 ccgaccagtg cgtcgaacca gtcgtcgctt ttgccttgcg cgagcagggt ttcggcggtt
     9961 gcgtcaacgg cttggaaaat ttgtgcgtcc agtgcttttg caaaggcttg tgcggcggcg
    10021 gaggcgtcca gtccggcaga tacgggtacg atgagtactt ttgccatgat atatcctttc
    10081 gtatgctgcg gtgtgcggca tatgtggttg gaaggggcgg catataggca gaaacggctg
    10141 cctgcgtgcc gtgcgtgccg tgtttggctt gaggcgcgca ggttgaatat agcaaacaaa
    10201 ttctgtttcc aacaagataa atatccgcag gcttgtggat gctgccgcct ttcagagggt
    10261 atttccgggg aagaacaggg cgggaccgtc caaatggagg acggcggaaa tgccgtctga
    10321 cagggtaggg gcggcgggga ggttgagcgt gaggacggtt tgtccggccc ggaggctgat
    10381 ttcggtgtgc cgtgctttgg gcgtggtttt gagaaccacg gcgtgaatgg cggcggcggg
    10441 tgcggaatgg gggtaaaggc tgaactgttc cggacggatg agcagtgtgc cgcgcgtgcc
    10501 tgcgggtgcg gcgctttgga cgggcaggcg gcccaatccg caatcggcgg tgccgtcggc
    10561 gttgagcgcg gcggggaaca cgatgccttc gccgataaac agggcggcat caaggtcggc
    10621 aggttgtcgg tacaattcgt gagggcttgc agtttggagg atgcgcccct gtttcatcac
    10681 ggcaatccgg tcggcgtatt gcagggcttc ttcgcggtcg tggctgacga aaacggcaga
    10741 tttgccgttg gcgcgcaggg cggcaatcat gtcttcgcga atctggcggc gcaactgttc
    10801 gtccagcgcg ctgaagggtt cgtccaacaa aatcagttcg ggatcgggtg cgagggcgcg
    10861 ggcgagggcg acgcgctgtt gctgtccgcc cgaaagttcg tgcggatagc gtccggcaag
    10921 ttcggaaatg ccggtcaatt ccaacatagc ttcgatgcgc tgccgctctt gcgccgtctt
    10981 gcctttgccg ttgcccagcc cgtaggcggt gttgcggtaa acggtcaggt gggggaacag
    11041 cacaccttcc tgtacgacat aacccaaacg gcgttcgcgg acggggaggt tggtattttt
    11101 cgagaagatg gttctgccgg aaagcgaaat ttcgccaaaa tcgggttgtt caaaaccggc
    11161 aaggcagcgt aaaagggtgg ttttgccgca gccggacgcg ccgacgataa agaggatttc
    11221 gcccgggtcg aggctgagcg aaatgtcgtt taaaactggg gtgttttgaa aacttttgga
    11281 caggtgtccg atgtgcaggg cggcggtcat ggcggtactt cctcaagctg ttatttgaag
    11341 gcgtatttct tcagcaggaa tacggggatg ccggaaaata ataccagcat cagcgcgtaa
    11401 ggggtggcgg cggcgtattg tgcgtccgat gtgtattccc aaacggcggt ggagagtgtg
    11461 tggacatcgt cggtggtcag cagcagggtg gcggtcagct ctttcatcag tttgaggaag
    11521 acgagtgcga atgcggcggt aatgccgggc aggatggacg gcagtaccaa cgtcctgaaa
    11581 ataaagaagt gtccgcgccc caatgttgcg ccgacctgtt ccatcccttt tgggagttgt
    11641 tccaaggaag tcctcagggt ggtttgcgcc atcggcaggt aaagcatgaa ataggcaagg
    11701 atgacgacga taaaggtttg gtaaacggca ggggtgtagt tgatgctgaa ataaaccaag
    11761 gatagggcga taaccaaacc ggggacggcg tgcagtaaaa acggcagcct gtctatccaa
    11821 acggttaaaa aattgcgata gcgaaccgat gcccaaacaa ggggcaaggc acataatata
    11881 gtcaaaatcg cacctaaagc cgatacgctt aaggaacgga taaaggcatc aaatacggat
    11941 acgagcgcga atgtgccgga agtgccgacc atcatccaat gtatcaatac gccaaagggg
    12001 ataataatgc ccaaagtcaa caagctgctt aaaaaaacaa tcgcgccaat ctgaccgggc
    12061 agtttgaggg ttttgacggg ataaggacgg gcaacgcctt tgccgctgtg gtaaatcttg
    12121 gctttgccgc gaaatatgct ttctccaaat acgacgatgc cgcacaccgc cattaaaaca
    12181 gcggaaagca gggcggcggt attgttgttg taggacattt cgtattcttg gaaaatggcg
    12241 gtggtaaaag tggggtagtt caaaatagat accgcgccaa attcgaccag catatgcagg
    12301 gcaatcagta acacgctgct gccgatggcg ggtttgagct gggggaggat ggcggaaaaa
    12361 aaggtttgca ggcggctttt gcccaaggac aggctgactt cttcgtaaga caggctgatg
    12421 cgtttgagtg ccgcctcgac gggcaggtag gcgagcggga acgaggacag gctcataatc
    12481 atcactgtcc cccaaaagcc ttcgacacgg aaggtcaggc tgatccaggt gaaacagctg
    12541 acaaatgcgg ggatgcacaa aggcagggtg attgccgtct gaaaaaaggt tttgccgaag
    12601 aagcggtaac gttggaacaa aagggcgcag gcaatgccca aaacaatgga aatcagggta
    12661 acgcccgcca tcatcgtcaa ggtgttggag agcaaatccc acatacgcgg gcggaacaac
    12721 agttcgacgg cgcggttgat gccgacctgc cacgaacgca tagcgacata taaaaaaggc
    12781 agggtaagcg gtagggcaat cagtaggatg aggccggtaa gccaaatggg tattttttta
    12841 ggagacatag tgttttttat cggcaaaacg ggcggacagt ataaatgtcc acccgtttga
    12901 caatccgaaa acggcttatt tcataccggc ttgctcaagc agccgggtgg cgtgttcttt
    12961 ttcggaaaca gtggtggcgg acacttgggg tgcttccaac ttggcgatgg gttccaaatt
    13021 gaaagtggat accacgtgcg gattcaaagg atattcggca cggacggcgg tcagggcgcg
    13081 ctgtccttcc ttgctggcga ggaaggcgac gaattttttc gcctcatcct tgttttggga
    13141 ggattttaac acggctgcgc cggaataggt aacgagtgcg ccgggatctc tgtggcggac
    13201 gaaattcagg cgggtgtgga cattttgtac gcctttttca cgcgcaaaag cgtgccagta
    13261 gtagttgttg atgagggcgg catcgatttc gccgttttca accgcttgaa gggcgacgga
    13321 gtttttagcg taaggcttgc cgtattcttt cagacctttg agccatttca atgcggccgc
    13381 ttcgcctttc agtttgacga tggcgacaac ctgttccaag aacgcgccgg aagtgggggc
    13441 gtaaccgatg cggtttttcc atttcggcgt ggcgtaattc aggacggatt tttccaaatc
    13501 tttttcagac agtttgcggg tgtcgtaaac gacgacgcgc gaacgtccgc tcagtgccac
    13561 ccagtctttt ttggcggcaa ccggcacgcc cttgccgcgt gtttcgttga tggtggaggc
    13621 gggcaggggc tctaggaggt tggctgcgga aagggtggcg agtgccggga tttgttcgga
    13681 atagaatacg tcggcggggc ttcggctgcc ttcttctttg atttgaccgg caagctggtc
    13741 gcctttggcg ctgttgagtt tgactttgat gccggtagcc cgggtaaagg catctgcaac
    13801 ggcttgtgcc gcttctttgt gttggccgtt gtacacggta atgtctgcca gcgcgggggt
    13861 tgcggcggtc agggctgcgg caagcagtgc gtatcggata gatgttttca tatcgatttt
    13921 ctcctaatga atgagagtgt acaccttgtt aagacataac ggggtgtagt gtattccttc
    13981 ttctttataa atgcaaataa ttatttttta aatttgttat tatccgatcc ggttattgtt
    14041 tgttctggct tgtatttttt tccgtgcatc gcgcccgtaa ggcggaagcg gagggtaatg
    14101 tgtggcggaa tgtgggtaaa ggcggcatct tgatttgccg gaatacttga gaacccttcc
    14161 ctttaaaacg ctgtctgaac agggttgccg gaatagtatt gccatcccag cagatacagt
    14221 ttgtcggggt cttgccaata ttgttcatcc agactgttca gcagtgagac ggttttgttg
    14281 tcgagatgtt gcgttatatc ccacatttct tttaggtttt tgccttccga ttggaggtgg
    14341 cggatttctt tgtccaatgt gtctgccgcc cgatggatca gcattgcgct gtgtatcgcg
    14401 ccgatttttt tcagaacgga acaagtcttt tcggcggcgg ggaaactcca gttgcagaca
    14461 aattgcagta tgccgccgtt gccgatatcg gcttctgccc gccaaattaa aaccagctcc
    14521 tgttcccacc cgtccatgct ttcgagtttg ccgtcatgct gttcaaaaag tttgtccacc
    14581 gcctgccaca tcatctgttc gaagcggtcg gcttgggtat ccgtatcggt catagtgttc
    14641 ttgtctttta aaaatgccgt ctgaacattt cttcagacgg catcgggggt taagctgaca
    14701 tttcccgcca gcgtttgact tggaaacgga cttgttcagg cgcggtaccg cccaagtggt
    14761 tgcgcgcgtt taagctgcct tcgggtgtca gcacgccgta aacgtcgtcg gcaatcaaat
    14821 cgctgaaacc ttgtaagact tcgagcggca gtttgctcaa atcgacaccc gcttggtcgg
    14881 cgtggcgcac ggcttgggcg acgacttcgt gggcatcgcg gaaaggcatg ccttttttta
    14941 ccaagtaatc tgccaaatcg gtggcggtag cgaagccctg catcacggcg gcgcgcatat
    15001 tgtcgggttt gacggttacg ccgcgcatca tatcggcgta aatccgcaac gtgtcgataa
    15061 gcgtgtcggc ggtgtcgaac aagggttctt tgtcttcctg attgtctttg ttgtacgcca
    15121 agggctgcga tttcatcagg gtaatcaagc cgataaggtg tccgatgact cggccggatt
    15181 tgccgcgcac gagttcgggc acgtcggggt ttttcttctg cggcatgatg gacgaacctg
    15241 tgcagaaacg gtcggcgatg tcgataaagc cgaaacgcgg actcatccac aaaatcaatt
    15301 cttcagacag gcggctcagg tgaaccataa ccagcgaggc ggcggcggtg aactcgatgg
    15361 caaaatcgcg gtcggatacg gcatcgagcg agttttggca gatttgttca aagcccaaca
    15421 gtttggcggt gatttcgcgc cgaatcggat aagtcgtacc ggcaagggcg gctgcgccga
    15481 gcggcatacg gttgacgcgg cggcggcagt ccgccatccg ttcgttatcg cgtccgagca
    15541 tttcgacgta ggcgagcata tggtgtccga agctgacggg ctgggcgact tgcaggtggg
    15601 taaagcctgg catgacggtt tcggcgtttt gttccgccaa atccagcaat gccgtctgaa
    15661 ggctttgaat caggcttcgg atgacggtaa tctggtcgcg cagccaaagg cggatgtcgg
    15721 tggcgacttg gtcgttgcgg ctgcggccgg tgtgcaggcg tttgcccgcg tcgccgattt
    15781 tgtcggtcag gcggcgttcg atgttcatgt ggacatcttc caaatcggac gaccattcga
    15841 ttttgccgct gcggatttct tcgaggattt ccgccatgcc ccgacggatg tccgccaaat
    15901 cgccttcgtc caacacgccg gtttctttca acatttgcgc gtgtgccagc gagccttgaa
    15961 tgtcccattc ggcaagccgt cggtcgaaac cgatggaggc ggtgtattgt ttgacgagtt
    16021 cggaaacggg ttcgttgaaa cgtccggacc aggttttgtc gtgcataagg attccttgat
    16081 ggggttattc ggtgcggtat ttttccaaaa ggcggcggaa gggttcgccg gtttcggggt
    16141 gtttcaggcc gtaggcgacg gtggcttcga ggtagcccag tttgctgccg cagtcgtagc
    16201 gcgtgccttc aaaggggtgc gccaggacaa attcgtgatc gagcagcttg gcgatgccgt
    16261 ctgtaagctg gatttcgttg cccgcgccgc gcggcagtcc ggtcagtaag tcgaaaatgc
    16321 gcggggtaag gatgtagcgt ccgacgacgg caaggttgga gggcgcgtct tcgggcttgg
    16381 gtttttcgac aatgccgata atgcgttgga actgtttgag ctgttcggtt tcgacgatgc
    16441 cgtatgagcc ggtttgcgat gcttcaacgg tttctacgcc caaaatgctg ttgccgctgc
    16501 gcccgtacac ttcgaccatt tgtttgagcg cgcctttggg ggcatcaatc aggtcgtcgg
    16561 caaggataac ggcaaagggt tcgtctccga tggcggcgcg ggcgcacaag acggcgtgtc
    16621 ccaagcccag tgcttccgcc tgacggatgt agaggcaggt aatgttcggc ggcaggatgt
    16681 tgcggacgtg ttccaacaat ttgtctttat ggcgcatttc caactcggtt tcgagttcgt
    16741 atgccttgtc gaaatggtct tcgatgctgc gtttgttgcg tccggtaaca aacaccattt
    16801 ccgtgcagcc ggcttccacg gcttcttcta cggcgtattg gatcagcggc ttgtcgacga
    16861 tgggcagcat ttctttcggg cttgccttgg tggcgggcag gaagcgtgtt cccatccctg
    16921 cgacggggaa aacggctttc cgtatgggtt tcattctttt tcctttgtat tgttttgatg
    16981 tttaaagggc gagtttgcgt aagagttcgg caagtgcctg cgcgcggtgg ctttcgcggt
    17041 ttttgacctc cgaatccaat tcggcggcgg ttttgccgtg tccgggcaga taaaaatacg
    17101 ggtcgtaacc gaaaccgttt tcaccggcgg gaacgccgtt ccattgtccg tgccatacgc
    17161 cttcggcaat aatcggacgc gggtcgtctt tatggcggac aaaaaccaat acgcagacgt
    17221 agcaacagct tttgtcggct ttgccggcaa gttcggcggc aagtttcagg ttgttggcgg
    17281 tatcggattt gggattgctg ccggcgtaac gtgcggaatg tatgcccgga gcaccgttta
    17341 aggcggcggc acagatgccg ctgtcgtcgg cgagtgcggg cagcccgctg tgtccggcgg
    17401 cgtgccgggc ttttgccagc gcattttcaa caaaggtgga atagggttcg gggcattcgg
    17461 gtatgctgaa ctcggattgc ggcaatacgg taatgccgta tggttcgaat aggttgccga
    17521 attctttgag tttgcctgca ttgccgcttg ccaaaacgat tttttccggt ttttcagaca
    17581 tagcggtttt cctttgcggc ggattgggcg gcgcgtaggg atttgtgccg caggtagagg
    17641 gcgaggctgc cgatttgtcc gaacagtgcg ccgaatgcga aaagaaagga ggcgacggcg
    17701 aaggctttgc tgccgacggt cagcagataa gcacccaaga ggacgagcag cataaatgcc
    17761 agtgtgaaga gggcaacaga caacagatag atttttcggc ggttcatggc gttcggtcgg
    17821 aaacggtatg ttcggattat agccgattgg gacggtattc cctagagctt ggaaaaatgc
    17881 cgtctgaaac ggcgttcaga cggcatggcg ggcgttatgc ctgtttgttc caacgttcga
    17941 tggattcttt gatgactttt ttcgcttctt ccgcatcgcc ccacgattcg actttggtcg
    18001 tacctgcttt tttcaggtct ttgtaatggt tgaagtggaa ctcgatttgt ttgatgagct
    18061 gttgcggcaa atcggacaaa gttttgtagg cgttgccgtt gttgcggtcg tcggcaggaa
    18121 cgcagacgat tttgtcgtct acttcgccgt cgtcaacgaa tttcatcacg ccgataacgc
    18181 gcgcttccaa gaatacgccg gttgccaaag gttgttcggt aacgagcagc acgtccaatt
    18241 cgtcgccgtc ttcgtccaaa gtttggggaa tgaagccgta gttggtcggt ttggcgaaga
    18301 tggcgggttc gacgcggtcg agttggaatg cggcgagttt gcggttccac tcgattttgt
    18361 ggttgctgcc ggcggggatt tcgttgacga cgttgatgat gccgccgtcc acatcgccgg
    18421 gggtcaggat ttggttgaag tctgccattt ggtttccttt gtttgggaaa gtttgaagtt
    18481 tgaaagtata gcacaaacgt ccggctgaaa atgcgcccga tgccgtctga aagggtgtac
    18541 gggcgcgtgt taccgtttgc ccaaaaacct gcccagttcc agaatcgcgc gcctgttgga
    18601 cggggaaaac actttttccg ccgcttcttc caagccgaac cagccgtagg agacgtgttc
    18661 ctcgggttgc aggacgacgg gcgtatcacg cgggatttcg gcagagaaga ggtgttcgcg
    18721 gttttcaaac acgccttttg gatagcggtg ccgccagtgg tggtagattt cgtaaaccgt
    18781 gctgtcgtgc cagtcttgaa gctgcccgtc cgccagcagg atgccggttt cttcccaaac
    18841 ttcgcgcctt gccgtttgtg cgacggtttc gcccggttcg aggctgccgg ttaccgactg
    18901 ccaaaatcct tccggatgcg tgcgttcgat gagcaggatg ccgccgtccc cgctataaag
    18961 gacgaccagt gcggaaacgg ggtatttgag cggttttgcc atcggcatct ttcggcgggc
    19021 tgcggtaatg aaggggcgga ttatagcaaa cgccgcacgt tatggcgttt atccttttcc
    19081 gtatcctttt ttctgcacgg atgggacgcg ccggtgtttg ccggtaaatt ttccgttgtg
    19141 tcaaaaagat aagggcggtt gtgattttaa tgcttgccaa agcgtcgggc ggaaactata
    19201 atccgcaact tatcgagtcg gagtgtggcg cagtctggta gcgcacttgc atggggtgca
    19261 aggggtcgaa ggttcgaatc ctttcactcc gaccaaaaat tccgaaagcc gctttcaaaa
    19321 gcggcttttt tgccgtccgt atgattatga tgtagagtac gcggcgacag acattcaaat
    19381 gccgtctgaa aaccgttcag atggcatctc tttatcttag tttcattccg taccatctta
    19441 aggaacatca aattgggcat ttcccgcaaa atatccctta ttctgtccat actggcagtg
    19501 tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg attcttttat ccggcaggtt
    19561 ctcgaccgtc agcatttcga acccgacggg aaataccacc tattcggcag caggggggaa
    19621 cttgccgagc gcagcggcca tatcggattg ggaaacatac aaagccatca gttgggcaac
    19681 ctgatgattc aacaggcggc cattaaagga aatatcggct acattgtccg cttttccgat
    19741 cacgggcacg aagtccattc ccccttcgac aaccatgcct cacattccga ttctgatgaa
    19801 gccggtagtc ccgttgacgg attcagcctt taccgcatcc attgggacgg atacgaacac
    19861 catcccgccg acggctatga cgggccacag ggcggcggct atcccgctcc caaaggcgcg
    19921 agggatatat acagctacga cataaaaggc gttgcccaaa atatccgcct caacctgacc
    19981 gacaaccgca gcaccggaca acggcttgtc gaccgtttcc acaataccgg tagtatgctg
    20041 acgcaaggag taggcgacgg attcaaacgc gccacccgat acagccccga gctggacaga
    20101 tcgggcaatg ccgccgaagc tttcaacggc actgcagata tcgtcaaaaa catcatcggc
    20161 gcggcaggag aaattgttgg cgcaggcgat gccgtgcagg gtataagcga aggctcaaac
    20221 attgctgtta tgcacggctt gggtctgctt tccaccgaaa acaagatggc gcgcatcaac
    20281 gatttggccg atatggcgca actcaaagac tatgccgcag cagccatccg cgattgggca
    20341 gtccaaaacc ccaatgccgc acaaggcata gaagccgtca gcaatatctt tacggcagtc
    20401 atccccgtca aagggattgg agctgttcgg ggaaaatacg gcttgggcgg catcacggca
    20461 catcctatca agcggtcgca gatgggcgcg atcgcattgc cgaaagggaa atccgccgtc
    20521 agcgacaatt ttgccgatgc ggcatacgcc aaatacccgt ccccttacca ttcccgaaat
    20581 atccgttcaa acttggagca gcgttacggc aaagaaaaca tcacctcctc aaccgtgccg
    20641 ccgtcaaacg gaaagaatgt gaaactggca aacaaacgcc acccgaagac caaagtgccg
    20701 tttgacggta aagggtttcc gaattttgaa aaagacgtaa aatacgatac gagaattaat
    20761 accgctgtac cacaagtgaa tcctatagat gaacccgtct ttaatcctaa aggttctgtc
    20821 ggatcggctc attcttggtc tataactgcc agaattcaat acgcaaaatt accaaggcaa
    20881 ggtagaatca gatatatccc acctaaaaat tactctcctt cagcaccgct accaaaagga
    20941 cctaataatg gatatagtgc cgggaattta agcaaaaaag tcctgacttt agtttacata
    21001 aacggcaaac tcaaattcgg gccaagcagt ctgccgtctt cggcattgaa agcacctgtt
    21061 cccattgtcg gcaaggctga ggctccgccc ggaaaagctg tgcctgtcgg cgaagcccgc
    21121 attccttcaa atccaaaagc ggtaaaagga acaaccgtta ccctgtcgca ggaacaacgc
    21181 ctagccaacg gagagctgat accgaaaggc tctgtggtaa cgtggtcgga caatgcggtg
    21241 aaggttgtgc ggcctgatgg gaaaaagcgg agatttaata gtcgggattg ggaacagaag
    21301 gcattgccga aaccgcaact accgaaagat tacagagatg acatcaacat aggtggaaat
    21361 aatcctagat ttaatcagca acggcatgaa aaaatcgaat cccacaaccc aaacagaata
    21421 gaggagatac gccaacgtaa tgccgtttct tctgcagaaa ggcaggctga tgcattaaaa
    21481 aggatgcaaa cgtctggccg cacccgcctg cctgggcagc cacaaaaagg aaaacccggc
    21541 gctgctagag aaatgtcctc ttcagacaat ccgaatcaaa cagccgaggc ttttgcacgt
    21601 cgatatttag gcaggaaacc tacactaatg gaacgagtcg aaggtgaaaa attatacgga
    21661 ggtaaatgtg tgggctgttg gaaagcgcaa atgccggacg gaacctttgt ggtgtatagg
    21721 ccagcaggta atgcttctat gaccaaggga actactgcgt ctgttgatat taataaccca
    21781 acattaaata acttaaatat taatttaaaa ggcaagccaa cagagttaaa atttaaattc
    21841 cctgctagat aaggatgttt taatgaattt agagacagca gaaaatattc aatgcgaatt
    21901 tgagtattta gcattagatg gttattttcc aatgcatttt gcatcgcatg gtcaaggaaa
    21961 caaagattgg caatttgcag ttgaattcat atatagatta ttaatatgtc aattagcaac
    22021 tcttgaacct atcaactttg aaacgaaaaa tgatatttta gacttttgcc ataatttagc
    22081 aaaacaaagt ccttttaata atgataatga ggtgtggtat cagggggaaa ttgtattaac
    22141 aaaaaaaggg atagatttaa taaaggaata tattccagag gcgtttgaag agtggaatgg
    22201 aaagaaattt gaattaaaca ttcctttcat taaaacatta aaaaatatat ttgttaatta
    22261 tgaggtagca tgggatgaga ataatccact attccccatt atatctttaa acctcggcac
    22321 ctgataaaca tggggtttat caagcgacag tggaaattaa aaagcctgat ggaagttggg
    22381 aaatgaaaaa aggaaggggc ggaaaaccta tgacaaatca ttctatgttc ccaaaagatt
    22441 gggatgaggc tagaattagg gctgaagtta cttcggcttg ggagaaaaga aaggaattag
    22501 aaggtcaaaa atggagaggt acaagtaaat ctggtgttga aatagaagga tttacggaac
    22561 ctaatcgaac agcttatcca ctttataata gtaaatgaag tattgattaa aaaggtgttt
    22621 ttaaatgtat aaaataaaat gtttttatga tcagtttagt tctggtgaat tatttaacta
    22681 ctgccagttt ttagataatt cttctaatca aaagataaat acacgaggaa cggtgtatca
    22741 atatattatt tatgttttaa caggagacct ttatctacaa aaagacatag atgaaaattt
    22801 agaatttatc catcaagcag aaaataatcc caatgaagtt tattcaggag gtggacaagg
    22861 tttttgctgg gatatttctg ctgaaaaagt agttttctat cacaacgaat ttgatgaaga
    22921 agacggctgg cctgatttaa gctgttcgct gcatactttt aaaactgctt taattgcttg
    22981 gaatgctttt ttgcaattgc ctaaaagtat tcattcggtg gtggagactg tgattgagga
    23041 ataagcataa ttagcttaat gaatagaatc agcgatatag attggactgc aaatccacgc
    23101 ttatacgctg tgccatgatt aacaggaaga gagcaacttg gatgggctag tagggatggt
    23161 aagcatttaa atatatcaat tgatggaaag attacacaca aatgactaat ttaaaattag
    23221 atttctactc tgaagttatt ataaaagatt cttgccctaa tgatttgtta gaaaatggtg
    23281 agactattaa aggaaaaaaa ggagtggtat taggtataag cgaagaagat ggtataatct
    23341 atggctatac tattttactt tttgatataa aatattgtat atatatagat aagaaatata
    23401 ttataccgac tggaaaaaaa ttctctcgcg atgattttta ttagctaatt taagtaattt
    23461 aagcaagttt tttagatact gaaactggta attatgttta tgtaagcata aggagttcag
    23521 attgaaggat tctacagggc aaatgaggct ggcaaccaag gatttggcgg aagccattaa
    23581 acgaggagaa gtacgtagtt ctgcttttac aacaaagcaa ctaaaggcaa ttgaaaaagg
    23641 caaagacaaa atccctagct acacttggca tcatcatcaa gacactggaa ggatgcaatt
    23701 gattaatgaa gacttgcatc atgataccgg ccatatcggt tggagagcga tgagtaaagg
    23761 aaagtaacta tgtggaaaat cataaaagag gatagtgatg atttaggatt tgcaattaaa
    23821 tgcttattct ctcagtctat tgatttaaat gaattcaagt tatggattga acaagtaata
    23881 cgcgatatgc ccatcgagga catccctttt tatatttttg acttggcgga ttttgatggg
    23941 ggaattgccg atattgacaa tattgtaggt tttgtttcga gttacagcct atcaaaatcg
    24001 aaaaaaaatg ccttgacagg cattgccttc ttaaggggga tagatgtcta tgatccgcct
    24061 atttcaaaag aaaaagcatt aaaagcctta aagaaacatc ctgaaattta tcagaagttt
    24121 cagcatttct ttccgtttgt agaactgccc ccgctttaaa cagtcaaaat gccgtctgaa
    24181 atgatattcg gctttcagac ggcatttttg acacaaagca ggtaaccaaa agagcgtttg
    24241 acggcaaagg aagataatta tgtgaaaaat tacgaaagag caaggtcaag atttaagttt
    24301 tgcaacatat tgcttattat cggcagcaat taatttgcaa gaatttaaat tatggttgga
    24361 gaaggttgtc ttagatatgc ctattgataa tatacctttt tatatttttg atttaattga
    24421 tcttaaagaa ggagtaggag atatttataa tattttggat tttgttccga attcagactt
    24481 atctaaagac caagatgacg cattaacagg cattgccttc ttaaggggga tagatgtcta
    24541 tgaccctccc gtttcaaaag aaaaagcatt aaaagcctta aagaaacatc ctgaaattta
    24601 tcagaggttt cagcatttct ttccgtttgt agaactgccc ccgctttaaa cagtcaaaat
    24661 accgtctgaa agccatttcc gccgctcaga cggcattttc gccccttt
//
LOCUS       DAWFBO010000009        24083 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFBO010000009 DAWFBO010000000
VERSION     DAWFBO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24083
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.009"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(431..2272)
                     /locus_tag="WGO87_000271"
     CDS             complement(431..2272)
                     /locus_tag="WGO87_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0391696.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGVLPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFVLGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANMP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            2363..2833
                     /locus_tag="WGO87_000272"
     CDS             2363..2833
                     /locus_tag="WGO87_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0391697.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAENQARLNPHEIA"
     gene            complement(2905..3717)
                     /gene="hpnC"
                     /locus_tag="WGO87_000273"
     CDS             complement(2905..3717)
                     /gene="hpnC"
                     /locus_tag="WGO87_000273"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0391698.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(3804..4286)
                     /locus_tag="WGO87_000274"
     CDS             complement(3804..4286)
                     /locus_tag="WGO87_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0391699.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDSELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            4759..5025
                     /locus_tag="WGO87_000275"
     CDS             4759..5025
                     /locus_tag="WGO87_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391700.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAPAA
                     EAPATEAPAAEAPAAEAAATEAPAAEAAASEAPAAEAPAAEAAK"
     gene            5135..6502
                     /locus_tag="WGO87_000276"
     CDS             5135..6502
                     /locus_tag="WGO87_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0391701.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYKHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELVGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(6683..8711)
                     /locus_tag="WGO87_000277"
                     /pseudo
     CDS             complement(6683..8711)
                     /locus_tag="WGO87_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9084..9530)
                     /gene="smpB"
                     /locus_tag="WGO87_000278"
     CDS             complement(9084..9530)
                     /gene="smpB"
                     /locus_tag="WGO87_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0391702.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(9585..10595)
                     /gene="waaF"
                     /locus_tag="WGO87_000279"
     CDS             complement(9585..10595)
                     /gene="waaF"
                     /locus_tag="WGO87_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0391703.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGCSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(10726..10801)
                     /locus_tag="WGO87_000280"
     tRNA            complement(10726..10801)
                     /locus_tag="WGO87_000280"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10766..10768),aa:Asn,seq:gtt)
     gene            complement(10992..11744)
                     /locus_tag="WGO87_000281"
     CDS             complement(10992..11744)
                     /locus_tag="WGO87_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0391704.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            12055..12579
                     /locus_tag="WGO87_000282"
     CDS             12055..12579
                     /locus_tag="WGO87_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0391705.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSATSA
                     ALCGTSSSVSTAAA"
     gene            complement(12668..12947)
                     /locus_tag="WGO87_000283"
                     /pseudo
     CDS             complement(12668..12947)
                     /locus_tag="WGO87_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(13318..14463)
                     /gene="dapE"
                     /locus_tag="WGO87_000284"
     CDS             complement(13318..14463)
                     /gene="dapE"
                     /locus_tag="WGO87_000284"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0391706.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTGIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGRQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTNVARAAIVETCGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLEDIPKLSAVYEGILARLLAGNAV"
     gene            complement(14563..15183)
                     /locus_tag="WGO87_000285"
     CDS             complement(14563..15183)
                     /locus_tag="WGO87_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HEZ0391707.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVVKWIAETVEDLLPLYEACHGA"
     gene            complement(15217..15726)
                     /locus_tag="WGO87_000286"
     CDS             complement(15217..15726)
                     /locus_tag="WGO87_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0391708.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLYGN
                     WDAVAAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGKTFIPKEKLTEIG
                     EIMAARRRR"
     gene            complement(15819..16385)
                     /gene="azu"
                     /locus_tag="WGO87_000287"
     CDS             complement(15819..16385)
                     /gene="azu"
                     /locus_tag="WGO87_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0391709.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPK
                     ASMGHNLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPA
                     KLADGDYKFACTFPGHGALMNGKITLVD"
     gene            complement(16666..17088)
                     /locus_tag="WGO87_000288"
     CDS             complement(16666..17088)
                     /locus_tag="WGO87_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0391710.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            17219..19969
                     /gene="secA"
                     /locus_tag="WGO87_000289"
     CDS             17219..19969
                     /gene="secA"
                     /locus_tag="WGO87_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0391711.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            19984..20136
                     /locus_tag="WGO87_000290"
     CDS             19984..20136
                     /locus_tag="WGO87_000290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391712.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            20114..21886
                     /locus_tag="WGO87_000291"
     CDS             20114..21886
                     /locus_tag="WGO87_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0391713.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            22073..24001
                     /gene="rpoD"
                     /locus_tag="WGO87_000292"
     CDS             22073..24001
                     /gene="rpoD"
                     /locus_tag="WGO87_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0391714.1"
                     /translation="MSRNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         6219 a         6721 c         5597 g         5546 t
ORIGIN      
        1 aatcggttcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
       61 atccactata tccgactttc catacttaaa cagcactgcc tcttttcaga cattgacggt
      121 tgcagccgcc cacctgaacc ttaatgattg actatttttt aatcatgtta ttattttcca
      181 taaaatacat gacattaaga tgtttttcca caaaagatac acacaccggc aaacaccggc
      241 tgtgtttatc ttttcttatg cctatttttt aatcatcgta tttttatctt ttaatttcaa
      301 tacgcaaact aacttataca cacggttttc acatctttgg actgcttccg tgtgtatagt
      361 ggatattgcc gttttccttt ctgacaaaaa tgccgtctga gaacttcaga cggcatttga
      421 aacatcggaa tcagcggttt tgttcatacc actcgataaa cttgtctgct ttgacaaaac
      481 ccagcagcgg ctcgctgcgg ctgccgtcgg agcggacgac aaacacgccc ggcggcccga
      541 acagaccgta ttctttcaac aacgcctgat gttcgggcat gttggcggtt acatcgattt
      601 ggaaaaagcg ttccatatcg actgcctgat gcacttccgg ctgattgagc gtgtaagccg
      661 ccatttcttt gcaggaaatg caccagtcgg cataaaaatc caaaacgacg ggtttgtcgg
      721 gatgttcttt caacgccgta tccatcgctg ccttcagcgc ggcagtatcg gcaaacattt
      781 ttccgtgttc cgaagatttg cctgcttcag ccggcggatt gagggtcagg aaatggtgca
      841 gcgcggtcgt tttgccgttt gcgccctgcc agccgaacca cgcgccgcct atcagcaata
      901 tgccgcccaa tacgaatgcc acagctttcg gacggcgttt ctgcctgcgt ccgttgacca
      961 gcagcataca ggcaggaacc agcatcagca gcgtgtacag cgcgacgacg agataatagg
     1021 gcaagtgcgg cgtggcgagg taaacggcga cggcaagcag gataaagccg aaagcgtatt
     1081 tgacggcatt catccaatcg cctgccttag gcaggatatg cccgccgaac gtgccgatgg
     1141 caatcagcgg aacgccggtg cccaacgcca aagtgtaaag tgccaaaccg cctaaaaccg
     1201 catcgcccgt ctgaccgatg tagcccaaag caaatgccag cggcggggcg acgcacggcc
     1261 cgacaatcag cgcggacaat atgcccataa taaagacgga aacgatttta ccgcctgaaa
     1321 gcctgctgct ttgattctga aaatacgact gcacggcgtt gggaagctgg atgttgaaca
     1381 gcccgaacat agacagtgcc aagacgacca ttaaagccga tgccgccaac accacccaag
     1441 cctgctgcaa ccatacggtc agcagtgcgc ccgtcagtcc ggcaacaatg ccgaccagcg
     1501 tataagtcag agccaaaccc tgaacataaa cgacggacag cacaaacgcc cgcgccttgc
     1561 ccgccttttt gtcgccgacc acaatactgg aaacaatcgg caacagggga tacatacagg
     1621 cggtaaaact cagacccaaa ccggcgagaa aaaacgccaa aagattggcg ttgagcgtat
     1681 cccaagacag cttgaaacgg ctgtcgccgc cctcatcccc cttcgggggc ggcagcaccc
     1741 cgctgccgtt ttgagaggaa ggctgcaaaa agcggtcttt ggcggatgcc ggttcgtcgg
     1801 tttgcggatg gtaagtgccg ttgccggaaa tatcaaactc ggtatccacg ggcggatagc
     1861 acacgccggc ttcggcacag ccctgatagg tcaaaaccaa tttatacggt tcgccgacag
     1921 cctttgcata aggaaaggca acctgcgctt cgtgatggta aaccgtctgc ctgccgaaaa
     1981 actcgtcttc cttctcttca cccttgctga aagaaggctg tcccaacaaa tccgccggat
     2041 cggtcttgcc gacgattttc gcctgataca tatagtatcc gtcggcaatc ctgaaacgga
     2101 cgttcacacc gtcgtcggca acggcaagct ccggcacgaa tgccttttcc ggcggcagca
     2161 gatcgttcgc atccagcgcg aaagctcgtc cgcacaacat caaaaatacg gcgaacaggc
     2221 aaatcagttt tttcataatc gaatccgttt cagacaaata atttgtctgc attataaatg
     2281 gtaaggttga cggtgggatt taatttatgt aaaacccgcc attatccgaa cctatttcca
     2341 taaacatctt atcgaacccg ccatgtacga tgtcaatacc cacgatgtcc gccgcttttt
     2401 cgcccgcgtg tggcagcagc ggctcaatcc gctgcaactg agcgcactgg aacagaaagc
     2461 cctccgcatt atcgaagccc atcccgaata ccaccgttat ctcgaacgca tcgaagacca
     2521 tctggacacc gactggctac ccgaaaacgg cgaaagcaac cccttcctgc atatgtcgct
     2581 gcatttgtcc gtccaagaac aggcgggcat agaccagccg cacggcatac gcgcaatcca
     2641 cgacaccctg tgcgccaaac gcggctggct ggaagccgaa cacgaaatga tggaggcact
     2701 ggcggaaaca ctgtggacgg cgcaacgcta cggcaccggt ttggatgtca atttctacat
     2761 gacccgactg cgcaaactca tcggcttggg tgcagaaaat caagccaggt tgaacccgca
     2821 tgaaatcgcc tgaccatacc aaccgcctgc aaaatgccat ctgaagcgga acaacccctt
     2881 tcagacggca tgcatttccc ccaatcattt ccacaacgcc tttttcagca taatcaacca
     2941 atccttctta tccaaaacgg ggcgttgtgc aaacacatcg tatcggcacg cgtccagttt
     3001 ctgcaaaatc aactgcgccc ccaacacaat catacggagt tccaaaccga tacgcccctt
     3061 cagttccctt gccaaaggcg aacccgcctt cagcatacgg aatgcgcgtc ggcactcata
     3121 cgccatcagc cgctgaaacg ccgcatccgc ccgtcctgcc gcgatctgtt cctcagaaac
     3181 accgaatttc aacaaatcgt cctgcgggat ataaaccctg cccttttgcc aatccacagc
     3241 cacatcctgc cagaaattga ccagttgcaa agccgtacaa atgccgtcgc tttgcgccac
     3301 gcacaccgca tccgttttcc cgtacaaagc cagcataatg cgtccgacag ggttggcgga
     3361 acgccgacaa taatcggcca gctcgccgaa atgcgcgtac cgcgttttga ccacatcctg
     3421 agaaaatgca gaaagcaaat cataaaacgg ctgcaaatcc aaaccgaacg gcacaaccgc
     3481 ctcggcatcc aatcgtgcaa tcaaaggatg cgccgaccgg ccgcccgatg ccaacacgtc
     3541 caactcgcgc cgcaaaccct ccaaccccgc caacctagct tcagacggca tactgccctc
     3601 gtccgccata tcgtccgccg tccgtgcaaa cgcatacacc gcatgaaccg gcttcctcaa
     3661 cctgcgcggc aaaatcagcg aaccgacggg aaaattttca taatgcccaa ccgacatacc
     3721 ttctccatcc atcaaacaaa atgccgtctg aaacggaaca aacccttttc agacggcatc
     3781 agatacctcc aagctgccgg caatcagtgg tggtgatgac cgtgcgggcc gtggacatga
     3841 ccgtgtgcga tttcctcatc ggatgcatcg cgcacgcttt caaccgtagc cttaaaacgg
     3901 attttcatgc ctgccaaagg atggttgccg tccaccaccg ccttgccgtc ggcaacatcg
     3961 gttacacgat agacgacaac atcgccggtt tcaggatcgt cggcttcaaa catcatgccg
     4021 acttcgactt caacagggaa cacgcccaca tcttcaatac gaaccaactc cgaatcctgc
     4081 tcgccgaacg catcgtcggg cgaaagcgcc acatcgaccg tatcgccggc atccttaccg
     4141 tgcaacgcct cttccaccaa agggaaaatg ccgtcgtaac cgccgtgcag atacgcaatc
     4201 ggttcttcgg ttttgtccaa aagctgattg ttggcatcat acatctcata atgcagcgaa
     4261 accacggaat ttttcacgat agccatattt gtcctttcag gaacagcaga ttgattacag
     4321 gcgcattcta acacaaccgc cgcgccggcc gattaccgtt aacctgttca taaactgtac
     4381 agcacatatt tcaatgtaaa tttttgttat tttattgcgg tgtaactttt ttacaacatt
     4441 cttaaaacca ttccgatctg tctgccgact ttcccaatcc gccttaataa atcatacaag
     4501 atactgaaat tatattaatc tctataatat ttatccctat cgaattttta acagcaaaac
     4561 cgttttacag gatttatcaa tccgcccgcc agaaaacttt tcattcaaac ctttttccca
     4621 tctgtacgac attgcaatcc cttattccat agtgcataat tacgcaaatt cagcgatgaa
     4681 tttccaaccc ggtttgtagt atggtcgata aagacctatt tgtttcaata atttaaattg
     4741 gttctaaagg ttactaaaat gaaaaaatcc ctgtttgccg ctgctttgtt gtctttggtc
     4801 ctggcagcct gcggcggtga aaaagccgct gaagctcccg ctactgaagc acctgccgcc
     4861 gaagctcccg ctgctgaagc acctgccgcc gaagctcctg ctactgaagc acctgccgcc
     4921 gaagctcccg ctgctgaagc tgccgctacc gaagcacctg ccgctgaagc tgccgcttct
     4981 gaggctcctg ccgctgaagc tcctgctgcc gaagctgcaa aataagcatt ttccgcttgc
     5041 aaaaaagcag gatacgttca gtatcctgct tttttgattt ttcagacggc atcagattcc
     5101 cttcctcaat cttctcccta cccttccgac aaacatgcct gaccttcata ccgaattttc
     5161 ccgactcctg ccggcagatg aaattgccga actttctccg acgcttttaa aagaccagcg
     5221 caaccgcttt acgtctgcac cagacatcat tttgcagccg cgcagcgttg aaagcgtgca
     5281 aaccattatg cgtttttgtt acaaacaccg tattccggtt acgccgcaag gcggcaatac
     5341 cggtttgtgc ggcgcggcag tatcggaaaa cggcgtattg ctgaaccttt ccaaactcaa
     5401 ccgcatccgc agcatcaatt tgtcagacaa ctgcataacc gtcgaagcag gttccgtact
     5461 ccaaaccgtc caacaggcag ccgaagcctc aaacaggctg ttcccactca gtctcgccag
     5521 cgaaggctcg tgccaaatcg gcggcaacat cgcctgcaat gccggaggtt tgaacgtatt
     5581 gcgttacggc acgatgcgcg acctggttat cggtttggaa gtcgtcctcc ccaacggcga
     5641 actggtttcc catctccatc ccctgcataa aaacaccacc ggctacgacc tgcgccatct
     5701 gtttatcggc agcgaaggca cactgggcat catcactgcc gccacactca agctttttgc
     5761 ccgtccctcg gacaaggcaa cggcatgggt cggcataccc gacatcgaat ccgccgtccg
     5821 cctgctgacc gaaacccaag ctcactttgc cgaacgccta tgcagttttg aactggtcgg
     5881 ccgttttgcc gccgagttgt cttccgaatt cagcaaattc cccctgccga cacattcaga
     5941 atggcatatt ttacttgagt tgaccgactc attacccgac agcaatcttg atgatcggct
     6001 tgtcgaattt ctttataaaa aaggatttac cgacagcgta ttggcacaaa gcgaacaaga
     6061 acgtatccat atgtgggcat tgcgcgaaaa catctccgca tcgcaacgca aactgggcac
     6121 cagcatcaaa cacgatattg ccattcctat cggacgcgtt gccgactttg tccgccggtg
     6181 cgccaaagat ttggaacaga attttaaagg catacaaatc gtctgcttcg gacatcttgg
     6241 cgacggcagc ctgcactaca atactttcct gcccgaaatc ctcagcaatg aagtctatcg
     6301 ttacgaaaac gacatcaacg acgcagttta tcgcaacgtt cttgcctgca acggcacgat
     6361 tgccgccgaa cacggcatag gtatcatcaa aaaacagtgg ctggacaaag tacgcacgcc
     6421 tgccgaaatc gccctgatga aaagcatcaa acaacacctt gatccatata acattatgaa
     6481 tccgggcaaa ctgcttccgt aaccggcatt tctgatttgc atacacaaca aagaaaggga
     6541 caatagatcc gattgtcggt ttagcgcgag ctcgtgagtg cggtaaaaaa ttggtggaaa
     6601 tgacacgaaa aatgaccgca tttttaaaat aaaaaaatcg gcagtaaatt tctctgccga
     6661 ttttattttg ttacaactta acttaaaacg tccactgtaa attcaacgca ccttgtttag
     6721 cctgatgatg tttgcctgtt tggcggttga atgttgcttg taaagttaag tgagatttga
     6781 ttttcactgc tacacctaat tggctctcaa ttgccgtctt attgtttatc actcgacgct
     6841 ctccgtctat ttccacaccg aaaggtttgt tgtggtaaag cgcgttcaca gcggcgaaag
     6901 gttcaatagc gatattttta tagagcgaaa attgcgcttt agcttgaacg ccaacccgag
     6961 tttgtaattg gcgggagcca agtaaattca cgtgggcatt ttcgctatcg ctgaattttc
     7021 cgtttacccc caaataagtc aattgtgcct gtggttgtag gtaaacacga aggctgttgc
     7081 cctttttagt gaagtgttcc gccaataaag cattgtaacc cgcttcaatt gaggcggtaa
     7141 taccttttga agtaaaacgt tctgtagcat cttcagtgtt aatgcggtgg cggaagcgtt
     7201 gatattgtac ccagctatcc gcatacgcac ctgtctgttt gtcctgaagc tgatgccaag
     7261 tggcgtaaac gcctgcacca aagcctttca catttcccgt tgtaagattg tctgtatctg
     7321 ggttgcggaa agtactgcgt tgttctgcct gtccgcccat taagccaata gaaagttgat
     7381 tactttcgtt ttgccatgtg aatacttcgc cgccgagttg cacacctttt cgatttccct
     7441 ctactggcgt ggttttacct tgcacccatt gcttagaatg tccgtcaatc acacgcaacc
     7501 acaagccttt gcgtggtaaa gtgcggtcaa aaatatcgct gtttttgtcg ttcaaacgca
     7561 aggcgaataa ggtattggcg gcttgagcct gttgtgcata aatcgaaata tcatcgcgtt
     7621 cttgcacttt ggtaaaaaag ccttctgggc gttgttgtaa agaaagcgta taaattccct
     7681 tttggtgttt gccagaaaga cggaatgcgt gtttatctgc tgtgccattt actttgataa
     7741 tttgatgccc gtcgagactt tttaaatcgt ctattggatt ttcgaagatg atgtcggaag
     7801 tgccagttat atttttttca aaagttaaaa ctggtgtttc agggacttta tcttttagac
     7861 cgtaagcaaa acgaaaacga gctccgccag cataatcttc ttttacgagt aaactttcac
     7921 ttttagtatt aaaacggatg tctgcattcg ttgtttttaa ttccccaaca tcagaatccc
     7981 aacggggctc ccagagagaa ttttctaagc ggaattcatc caaactaatc gtttgcccga
     8041 taacgtgcga gttgtctgta acctcaatat agtggaatgg atctacatca gaatagagat
     8101 gctcttgcga actagaaagc tgattaagtg ttgggcgaag ccattcgata taattaccta
     8161 cagataaaat ttcgctgttg atatggctat tttttatttg tggtgctaag gcgaaaatat
     8221 cgctctttat gcgtatcggg gcatagcttc ctaaggatgg ttgtctacac ttatcattat
     8281 tggaattccc agtatttaca cagtccttaa aatattgccg gagtggtttt tcttcttccc
     8341 catttctttt ttcaaccccg aaacctcggg cgaagccaac taggtaatct tcggtatatt
     8401 tttgatcata aaaagaaatc ttttttgagt tgttgatgtt ttcgaattgg tgtgttttag
     8461 agaatagtgc gggaactttg agagagttct gagagctacc attcgtcggt tttagaagat
     8521 aagtttcttg tttccagtat tcgctgcttt tatctctaag tttttttaag cgatcaattt
     8581 gctcatttgt taatttagaa ttgtcgtaaa cgtaatcaac agccataaag aggagaggta
     8641 taaagaatag aaaaaaatag acttacaata aatgattttt taaacttctg cttgcttgct
     8701 tcgagtttca taataaattt tcctttgtca agtaaagata aatggggcgt ggattttagc
     8761 atagaattga acaaaaaatg tcatttagct cacatttttc tctatttatt tcttatttat
     8821 taaaagtaaa cgtttgcttt ttgctatttt gtcaagccag tttgaaaatg tgtataattg
     8881 ccctcgttat tcacaaaaat ttcaggaaaa atgaccgcac tttacccttg gttaatgcca
     8941 atttatcatc aaattgctca aacctttgac gaaggtttgg ggcatcatgc cgtgctgatt
     9001 aaagcggatt ctggtttagg tgtagaacgt ttacacatca ggcggcagcc ttgcccatac
     9061 cgtctgaagc actgtttcca caatcagcgc gtatgcttaa tcaaccgctg tttctcgcgt
     9121 ttccaatccg cctctttcat actctgacgt ttgtcgtgct gtttcttacc ttttgccaaa
     9181 ccgatttcca tcttgatttt tccgcgcgaa aaatgcaaat ccagcggcac aatggtgtag
     9241 ccggcacgtt cggttttgcc gattaatttg ttgatttccg actggttcaa caagagcttg
     9301 cgcggacgta cggcatctgg tttaatgtgt gtcgaggctg tgggcaaagc cgtaatatgg
     9361 cagccgacca gataaaacgc gtcttttttc caatagatat aactctcttt aagctgtacg
     9421 cgcgcggcgc ggattgcttt gacttcccag ccttccaaga ccaaaccggc ttcaatccgg
     9481 tcttcaatga aaaaatcgtg aaatgctttt ttattgttcg caatagccat aaacatccta
     9541 tcaatatccg ccgtcagacg gcataaaccc gaaaacagaa cccatcatac cgcctcttca
     9601 accgcctgca caatcttctc gggatacagc ctgttgaggc agtcggtatg ccccaacgga
     9661 cattcacgtt tgaaacaggg cgaacattcc aagtgtaggc tgacgatttt cgccctgtcg
     9721 ctcaaaggcg gcgtatgcgt cgggctggaa gaaccgtaaa ccgccaccac tttcctgccc
     9781 aatgccgccg ccaagtgcat cagcccgctg tcgttgcaca cgaccgtgtc cgccaacgac
     9841 agcaaatcca ttgcctgcga caaatcggtt ttgccgcaca aattgacaca cataccgtct
     9901 gaaaggcggt tgatttcctc ggcaatttca tcatcttttt gcgaaccgaa caaccaaacc
     9961 tgccaacccg ccgccagata atgtttgccc aactcggcaa aatgccttgt cggccaacgc
    10021 tttgccggcc cgaattccgc acccggacaa aaagccagaa caggctttcc aagattcaaa
    10081 ccgaaagttt cgacagaaat ttcccgccgc cgttcatcaa tggaaaactc gggaaatcct
    10141 gaacacccgt caaaatcttc ctgactctga tgcgcgagag ccgtataccg gtccaccatc
    10201 aaaggcagac gttccttatc cagcctgcgt atatcgttca acagaaaata acggctttca
    10261 ccgacataac ccgtcctttt accgatacct gtcgccagcg cgatgattgc cgatttcaaa
    10321 gaaccgggca acacgataac ctgatcgtat ccgcgccgcc ccaaatccct accgacccgc
    10381 caacggcgtt tcaactccaa cgcaccgtgt ccgaacggat tttcaagaat ctcattcact
    10441 tccggcatac gctcaaacac cgccatcgac cacttcggtg cgaacacatc aatcgtgcaa
    10501 ccggggtgaa gttccttcaa acggcggaac aagggctggg tcatcacgca gtcgcctatc
    10561 caactgggag aaataatcag gattttgatg gacataacaa gaaaccgaaa tcagacaggc
    10621 agaattttac cgcgaaaccg ttggaaaacc tatcttgccg cattccgaac gccgtacgtg
    10681 caaatatgaa aaagcccgaa cattcaagtt cgggcttcaa aattctggct ccccgacctg
    10741 ggctcgaacc agggacctgc ggattaacag tccgtcgctc taccgactga gctatcgggg
    10801 aatggggcgt attatagcgt ccggaaaaaa tgtgtcaatc cttaattttg gaaaaatggg
    10861 cgacaaaacg acaagcatat gaatcagaaa gacattaaga ccgatgcctt aaaaggattg
    10921 ccgttgtatg aatttccaca gccgtcatca caccatattt aagcccgatg agccgttctg
    10981 ccctcccccg cttaaaacaa tgccgtctga acttcgccgc gttccaaagc cagtaaaaac
    11041 tgtttgcggt tcaacccgcc cgcgtagccg gtcagtttgc cgtcgctgcc gatgacgcgg
    11101 tggcagggaa tcaggacaga caccttgttc tgcccgttgg cggcggcaac ggcgcggacg
    11161 gctttggggt tgcccaaacg ctgcgcctgc tccttgtagc tgcgcgtttc gccgtaagga
    11221 atcgccaaga gcgcgtccca tgcctgcttt tgaaactcgg tgccaatctg ctccaaaggc
    11281 gtggcaaagg ttttcagacg acccttgaag tataagtcca attcctgccg caaaagttgc
    11341 gtccgctcat cctcccgaaa cacaaaccgt ccgcgcaagg ctttttggac ggcggcaatt
    11401 tcctgttcca aatgcttctg tccgacaaac tccagcagac acaatccctt atttccgaac
    11461 accgccagca tctcgcccaa aggcgtggca atggcggcac acaccagctc attcaaactg
    11521 tcgggataac gcgcttccaa cagacggatg gctcggcgga tacggacata ttcttcaggc
    11581 gcgcagccga tattgtccca aaaatcccga tcgaactgcc cggcttcgca ttccgtcaaa
    11641 ttgggatgcg gcataacgcc gcactcacaa acccgggttt cgagccaata gcggatttca
    11701 tcccattttg acggcagatt gtttaaggaa ggcagggtaa tcattcgttt gctccgtatc
    11761 tctatcatgg atttgacggc aaaattccca attttcgcca ttcccgcacg ctgatgcagg
    11821 aacgggctac gacgtaaatc ttgagggtta ggttgcggca atgcctaaat atgcgatatt
    11881 tccaaagcct cagggaaagg aattaaagac ttcagacgac ctgcaaaggc tcgtctgaaa
    11941 ccacgatttt tgcatttgcg cattctggca catcatccaa ccgtttcggc acattcctgc
    12001 cgccgttgac agcctataat gaatccactt attcatcaag caaaggaatc atctatgcaa
    12061 acccgcattc tctccgccgt actgctggct ttttcaaccg ctgcctttgc cgggggcgca
    12121 ttcacgctgc aattcgacaa cccgtccgaa gacggcggct tcacgcaaaa ccagcttttg
    12181 agcgcgcctt acggatttgg ctgttcgggc ggcaatgctt cgcccgcgct gtcgtggaaa
    12241 aatccgcccg ccgggacaaa aagtttcgtc ctgaccgttt acgataaaga cgcgccgacc
    12301 ggactgggct ggatgcaccg ggtggtcgcc gacattcccg ccgatgtcca ccgccgcaac
    12361 gcgacctcgc tgcaattaag ccgctgcgcc aacatcgccg acgaccagtc cgcagccata
    12421 tcggcggtaa tcagtttgcg gatttgccgc atcaggttga cgccttcgta cacggcaaaa
    12481 ccgatgccgt catgctgcaa ccacgccaac acgccgcaaa gcgcgacctc cgccgcattg
    12541 tgcggcactt cttcatccgt cagcaccgcc gccgcataat caaacgccgc gcccatccgc
    12601 ccggggtgcg gcagcttcac cgcatcgcac actgcctgcg ccgtcccgta ttgtgcggca
    12661 aacctttcta tggcttcctg ttcgaaagca atccattgcg cctgatagag gccgtctgaa
    12721 tcgggaatat taataacgtc aaacgtctgc ccgcctgcca aggcgaccgc ctcacccgcc
    12781 gcaatcctca attcgcgcct cgcccaccgc agcttcttgc ttccgcgccg cacgataagc
    12841 acagccggtt catataccgc cacgctgcgg tacaaggcgg tatgatgttg cacgatgccg
    12901 cccaaagcac ccaatcgttc gcgcgtatga aagtaatttg acggcactat tatctccaac
    12961 acgcagggcc gcacataagg cgcagccctg tttaccctga tttgggaggg ttacttccca
    13021 aaaataaacc aaaatcccac cgccccgaat agacgggttt caaccatatt ccgtcggcgt
    13081 acctgcaact tggctccctc ccctgtgagg gagggtcggg gagagggtaa aacggggcag
    13141 atacaggcaa tatttccgtt gccgccccaa tgccctctcc ctaaccctct ccctcgggag
    13201 agggaatgga ttgccgttga aataaatcgc tctacataaa aatcaatgcg ttatctcaaa
    13261 cccacgttag gtctaatcaa atggtcggac atccgtatcc gacagcccat cttcttttca
    13321 gacggcattt ccagccaaca accgcgccaa tatcccctca tacaccgctg acagcttcgg
    13381 aatatcttcc agccgcacgt tttcgttgat ttggtggatg gtggcattgg acgggcctaa
    13441 ttcgatgagt tctttcgcaa tggctttaat gaagcgtccg tccgaagtgc cgccggtggt
    13501 ggacagttcg gtctccacgc cgcaggtttc aacgatggct gcgcgtgcca cattggttag
    13561 tttgcccgca tgggtcagaa agggctgccc cgaacacgac cactgcaaat cgtattgcac
    13621 gccgtgtttg tccaaaatgg cgtggacgcg ttgtttcagc cctgcttcgg tggactcggt
    13681 ggagaagcgg aaattgaatt tgacgttcag ctcgcccgga atgacgttgg tcgcgcctgt
    13741 gccaccgttg atattggaaa tttggaaact ggtcggcgga aagtattcgt tgccttcgtc
    13801 ccatacttcc tgcgtcagct ctaacaaggc aggggcaaaa gtgtggacgg ggttcaccgc
    13861 caaatgagga taggcaatat gcccttgcct gcctttgacg gtcaggttgc ccgacagcga
    13921 gccgcgccgg ccgtttttaa tcatatcgcc caatttgtcc acggcggtcg gttcgccgac
    13981 gatgcagtag tcgataagct cgtcgcgcgc tttcaacaca tcgacgactt tggtcgtacc
    14041 gtccagcgcg tcgccctctt cgtcggaagt aatcaggagc gcaatgctgc cttgatggtc
    14101 gggatgttcg gcaacgaaac gttcgcaggc ggtaacaaaa caggcgatgc cggttttcat
    14161 gtccgccgcg ccgcgcccgt ataatcttcc gtcgcgctca gtcggttcga acgggggcga
    14221 atcccatttt tcaacagggc ccgtcggcac aacgtcggta tgccctgcaa aacagacgac
    14281 gggagctttc gtgccgcgtc gcaaccagat gtttttggtg tcgccgaaat ggagttcttc
    14341 agccgcaaaa ccgattttgt gcaggcgttc ggcaaggagt ttttggcaat ctcggtcgtc
    14401 gggggtaacg gatgggcggg aaatcaattc tttggcgagt tctagggatt gggtttcggt
    14461 catttttcgg cttttctgtt tgagatgccg tctgaacgga gtttcagacg gcatttgatg
    14521 ttgaatggaa cgggctaacg atagcgaagc gggttcttca ggtcaagcac catgacacgc
    14581 ttcgtaaagc ggcagcaaat cttccactgt ttccgctatc catttcacga catcctgctt
    14641 gcccaaatcg tcgcgctcga tgtgtttgcc gatgcagaag aaatcttcgt cgttttgcaa
    14701 ccttcggtcg gattcatttt gctgcgcgac ggtgcggtag tcgtcgtatt cgctttccgc
    14761 accgtgccac atatcgaatg cagcgtattt gtcggtatcg aaatcatcca gccagcggtt
    14821 gtattccggc agtgcaatcg gcgacacgtc ggctttgtag caatgccagt ccaagctgac
    14881 actcaaacgg cggcggttga gcagtatcga caaaatcgcg gcggaatttt tatattgttc
    14941 gtatttgaag taggcaaaga aatgggcgcg aacctgccag ccgttgcacc agcgttcgat
    15001 gtgcggcggc gcaaacggcg cacccaattc ggcggcaacc tgctgaatca gctgctgcca
    15061 tatctgccag ttttccttgt agtcggcttt gatttgcgga atgctttcag gctggtattt
    15121 tttaagctgg gaaaattgga aaaacgggat attgaacaaa tcgcaacttt tcggggtcag
    15181 cataatatat ccttgagacg attgtttcag acggcattat ctgcgccggc gcgccgccat
    15241 gatttcgccg atttcggtca gtttctcttt tgggataaag gttttgccca tgtcaaataa
    15301 gggttcttca atcgccaaat gaacatcata tcccgccaca aaacgtttga acgcttcctc
    15361 atcggggaca taagcgttgt ctgcttcgag tttggcaaat tcggcagcca cggcatccca
    15421 gttgccatac aggctcacat gctgacgcaa aagctcgtcc acgctttctt gggcttgcgg
    15481 cgcgtattgc agcagcagcg ggaagaagtt ttcttcttcg tcttcatggt gcagcggcgc
    15541 ggcaacgttg aaatactggg cgatttggcg gatggtttgc aaaacaatct gattgcagcc
    15601 gttttcggcg atatagtccg acagcatggc gacttgtccg cagaaacggc gcactttgcc
    15661 gtggcaggca tacagcattt caatcggttc ggcaaaggta acgcttttgg tttcaaacgg
    15721 attcatggtt tttaggtttt attcttaaat taacgtttcc agattataac aaaacagcct
    15781 gctcaaggca ggctgtctgt tttcaagctt taggcgaatt agtcaaccaa agtaatttta
    15841 ccgttcatca aagcaccgtg acccgggaag gtgcaggcaa atttgtagtc gccgtcagcc
    15901 aatttggcag gatccagagt cagggaagac tcttcgccgc cgccgatcag tttggtgtgg
    15961 gcaacaacgc gcgcatcgtc aggtttgaca tagtcggtat cggcagcacc tacgccgtct
    16021 ttaaatacgc cgtccatgtc ttcagctttg gcaatcacaa ggttgtgacc catgctggct
    16081 ttgggttgcg taccggtatg tttcagagtg atggtaaact ctttacatgc tttgctgact
    16141 tggatgtctt tggtgttgaa ctgcatattg tcgttggatt cgacagttgc cgcacagttg
    16201 ccggcagcag gggcttcggc agcatctgca ggagcagcat ctgcaggagc agcttcggcg
    16261 gcaggtgctt cggaagcggg tgcttcagca gcaggagttg cctcggcagc aggcgcggca
    16321 ggttcttgag agcaggcagc caaaccgata acggcagcag aaatcagagc cagatacgct
    16381 ttcataacaa atctccaatc gataaaataa tattcggttt tacaggaatc aaagtgcaac
    16441 cgccattaac aaaaccttga aaaagattcc gccgcgttgc acaaacagat gtttcggggt
    16501 ggcattttgc tacaaatttc atttaaaatc aaagcctgtt tgcaagttta caatcgttta
    16561 cccaaaaaag ggcaatttta ccccgaacct atttctttag tattagaccc attatccttt
    16621 acttcttaat attaacggat gtttacacaa attcccgtat acattttatg cgccatgcct
    16681 tctgaccaag tttgccaatg cctccgccaa ttcgggatgc cgtttttcca actttaccgc
    16741 cgccgaaccg aaactctcca gcgcagcctt actcaaatgc agggtattgg ttttcggcgg
    16801 tttttccggt ttcgggacca gcctgaccga aacagagcgt atcgaagcat caagccctgc
    16861 caactgcggc aataccgacg gtgcaatcat tttcaagcgc gatgccgcca tattgtttgc
    16921 cgccaaaagg acaagcctgc cgtcttcgat acatgccgtc tgaaaatgcg ggtgcaggtt
    16981 ggcaggcagc agttttttca cggcggcatc caaccgccgc cactgtcccg cctgtttcaa
    17041 aagtccggaa agcagcgcgt cccgcctgcc caactgttcc aaattcataa aacatacacc
    17101 caaaaagatt gaaataccgc aaacgcgcct ttatttcaga cggcattagc actttgcaca
    17161 aacgcttgtg ttaaaatcgc gttttcgccc actattatat caggcgcagg aattattcat
    17221 gctgacaaac attgccaaga aaatcttcgg cagccgcaac gaccgcttgc tgaaacaata
    17281 ccgtaaatcc gttgccagaa tcaacgcgct cgaagaacag atgcaagccc taagcgatgc
    17341 tgatctgcaa gccaaaactg ccgaattcaa acaacgcctc gccgacggtc agactttgga
    17401 cggcattttg cccgaagcct tcgccgtctg ccgcgaagcg tcccgccgca ccctcggtat
    17461 gcgccacttc gacgtgcagc ttatcggcgg tatggtgctg cacgacggca aaatcgccga
    17521 aatgcgtacc ggcgaaggca aaaccttggt cgccaccctc gccgtctatc tcaacgcgct
    17581 ggccggcaaa ggcgtacacg tcgttaccgt caacgactac ctcgcctcac gcgatgcggg
    17641 cattatggag ccgctctaca atttcctcgg ccttaccgtg ggcgtgatta tttcagatat
    17701 gcagccgttc gaccgtcaaa acgcctatgc cgccgatatc acctacggca ccaataatga
    17761 attcggcttc gactacctgc gcgacaatat ggttaccgac caatacgaca aagtgcagcg
    17821 cgaattgaat tttgccgttg tcgatgaagt ggattccatc ttgattgacg aagcgcgcac
    17881 tccgctgatt atctccggtc aggcggatga caacatccag ttgtaccaaa tcatgaacac
    17941 cgttccgccc cacctcgtcc gtcaagagac agaagaaggc gaaggcgact attgggtcga
    18001 cgaaaaggca catcaggtca tcctgagcga agcaggtcac gaacacgccg agcaaatcct
    18061 gacccaaatg ggattgctgg cagaaaacga ctccctctat tccgccgcca atatcgccct
    18121 gatgcaccac cttatggcgg cattgcgcgc gcattccctc ttccacaaag accaacatta
    18181 cgtcatccaa gacggcgaaa tcgtcatcgt ggacgaattc accggccggc tgatgtccgg
    18241 ccgccgctgg tcggagggtc tgcatcaagc cgtcgaagcc aaagaaggcg tggaaatcaa
    18301 acgcgaaaac caaacccttg cctccatcac cttccaaaac tatttccgcc tgtacaccaa
    18361 gctctccggc atgaccggca cagccgatac cgaagctttc gagttccaaa gcatctacaa
    18421 cctcgaaacc gtcatcatcc cgaccaaccg ccctgtacag cgcaaagact tcaacgacca
    18481 gattttccgt tccgccgaag aaaaattcga agccgtcgtc aaagacatcg aagaatgcca
    18541 caaacgcggg cagcccgtcc tcgtcggtac gaccagcatc gaaaactccg aactggtctc
    18601 ccaccttctg caaaaagccg gactgccaca caacgtcctc aacgccaaag aacacgaacg
    18661 cgaagcgctg attgtcgccc aggcaggcaa agtcggcgcg attaccgttg ccaccaatat
    18721 ggcgggacgc ggtacggaca tcgttttagg cggcaacctg aagcaccaaa ccgatgccat
    18781 ccgcgccgac gaaaccttga gcgacgaaga gaaacaggca caaatcgccg cactcgaaga
    18841 cggctggcag gcggaacacg acaaagtgat ggaagcaggc ggtttgcaca tcatcggtac
    18901 ggaacgccac gaaagccgcc gcatcgacaa ccaattgcgc ggacgttccg gccgtcaggg
    18961 cgaccccgga tccagccgct tctatctctc ctttgaagac ccattgctgc gcttattcgc
    19021 actcgaccgc gccgccgcca tcctcaaccg cctcgccccc gaacgcggcg tcgccatcga
    19081 acacaacctg ctgacgcgcc aaatcgaagg ggcgcaacgc aaagtcgaag gcagaaactt
    19141 cgatatgcgc aaacaggttt tggaatacga cgacgttgcc aacgagcaac gtaaagtcat
    19201 ttacagccaa cgcaacgaaa ttttaaccag caaagacatc agcgacctga tgaaggaaat
    19261 ccgttctgat gtcgtcagcg acctcgtgga tacctatatg ccgcccgaca gcatggaaga
    19321 acaatgggac atcccgactt tggagaaccg tctggctgcc gaattcagac tgcacgaaga
    19381 catccaatcc tggctgaagg cggacaatgc gattgacggt caagacatca aagaacgcct
    19441 gatcgaacgc atcgaaaacg aatatgccgc caaaaccgaa ctggtcggca agcaggcaat
    19501 ggccgatttc gagcgcaacg tgatgttgca ggtcatcgac aaccaatggc gcgaacacct
    19561 cgccgctatg gactacctgc gacaaggcat acacctgcgc agctatgccc aaaaaaatcc
    19621 gaagcaggaa tataaacgtg aagcctttac catgttccaa gacctgtgga acggcatcaa
    19681 attccatatt gcctccctgc ttacctcggt tcaaatcgaa caaaaccctg tcgcggtggt
    19741 tgaagagcaa cccatcggca acatccagtc catccattcc gaatcgcccg atatggaaga
    19801 acttttgggt cagtcgcaaa ccgatctggt taccgaagcc tttaatcccg atgggacaga
    19861 tttcagcccc gaagccttgg aagcgcgggg gcaaatcgtc caccgcaacg acccctgccc
    19921 ctgcggcagc ggtttgaaat acaaacaatg ccacggcaaa ctggcttaag cgtttgaacg
    19981 caaatgccgt ctgaacatcc cgctcccgtt tcagacggca ttttgcctga accgccacat
    20041 ccgactgcca ttccgaaaaa tcccgatttc gtaccgtccg taccaaaaac agacatcccg
    20101 tccgccccac atcatgattc catccgactt cattgacgag cttttagcca aaaccgatat
    20161 tgtcgatatt atcgacgagc aggttccgct gaaaaaaggc ggggcgaact atatggcgtg
    20221 ttgcccgttc cacaaggaaa aaacgccgtc gttttcggtc agtccaacca agcagtttta
    20281 ccattgtttc agttgcgggg cgcatggttc ggcgattggt tttgtgatgg agcatcaggg
    20341 gctgtcgttt ccggaggctg tgcagtttct tgccgaccgt gtgggcatgg ttgtgcctaa
    20401 agtgcgtggg cagaacgata atcccgaagt ccgtgccgaa cgtaagaaaa aacagcagac
    20461 gctggaagaa acgacggcgg cggcggctga tttttacgcg caacagctaa aattcaatcc
    20521 ggcggcaaaa gcttatttgg acaagcgcgg cttgagtgca gaagttatcg cgcattatgg
    20581 tttgggctac gcgcccgacg gctggcagcc tttggcgcaa gtgttccaac cgtatccgaa
    20641 taccgcgttg gtggatacgg ggatggtgat tgacaatgag gggcggtatt acgaccgctt
    20701 ccgccatcgg attatgttcc ctatccgcaa tccgcgcggg caggtcatcg gtttcggcgg
    20761 cagggtgctg gacgactcca aacccaaata tctaaactct cccgatacac ctttgtttga
    20821 caaagggaaa aacctttacg gcttgtatga ggggcgtgcc gctgtcaagg aagcggagcg
    20881 gattctggtg gtcgaaggct atatggacgt ggtcgcgctg gcacagttcg gcgtgggcta
    20941 cggtgtggcg gccttgggca cggcaacaac agctgaacac gtcaaaatcc taatgcgtca
    21001 ggcagacagt atttatttct gtttcgacgg cgacagcgcg gggcgaaaag cggcttggcg
    21061 cgcgctggaa aacgcgttgc cgcagttgaa agacgacaaa tcgctgcatt ttttgttcct
    21121 gcccgaggaa cacgaccccg acagttacat ccgtgcctac ggcaaagcgc aatttgaaga
    21181 cgcgcttctg aatcaaagca agcctttgtc ggagtatttc tgggaacacc tttcagacgg
    21241 cattcatctc aatacgcagg aaggcaaggc ggaattggtg aaaaccagtt cgccgctttt
    21301 ggcgcagatt accgcgccgg cattggctta tttgttaaaa caacggctta gcgaactggt
    21361 cggcatcgac cccgacaacc tcgcgcaact gctaggacag gaagcgccga agcggcacgt
    21421 caaacaaaaa aactacaaac tgcctccgat ttccgtcaaa cagcccgtca tgctgacgct
    21481 ggtacagcgg caaatccgca gcctcttgat aaatccggat tgggctgcat atatagacct
    21541 gcccgattat ctggcgttgg acggtgattt cgcctgcctt gccaatcttg ccgaatcgat
    21601 taaaaaccat gccgccgtac ccgaaaccgc tcaggtttta gagtatatgc gcggctcgcc
    21661 ttacgaagaa acgataaccc gaatcttcca ttcaacgcac caatcggaag aaatgaacag
    21721 cagcagtgaa gaagattgcg agaatttcca aatcggcatg aaaaaactgc tcaatgagtt
    21781 aaaatacagc caaatcgaaa cattaaaaca aaaaagcctg caatccggct taaatgaaag
    21841 cgagaaaaaa cttttgctgt cgttgctgac cgcaaaacaa aattgaccgg cggattccgc
    21901 cacccgtaaa cccgttatgc cgtctgaaaa gcacttaccc cggctgcaac aacgacacct
    21961 gcagaacacc catcccaaaa agccttcaga cggcatcaga gtacccgctc tgccacgcct
    22021 tcaggtgcgt tcaaacgcaa accgtcggca ccttaccaac agaaagcaga caatgtccag
    22081 aaaccaaaat cacgaagaat accaagacga tgcccgcccc ctgacgattg aggaacaacg
    22141 cgctcgcctg cgccaactca tcattatggg caaagaacgc ggctacatca cctactccga
    22201 aatcaacgac gcactgcccg acgatatgtc cgatgccgat caaatcgaca acatcgtcag
    22261 tatgatttcc ggtttgggta tccaagttac cgaacacgcc cccgatgcgg aagacatatt
    22321 gttaagcgac aatgccgccg ttaccgacga tgatgccgtc gaagaagccg aggccgccct
    22381 ttccagcgca gattccgagt tcggcaggac gaccgacccc gtccgtatgt atatgcgcga
    22441 aatgggacag gtcgacctgc tgacccgcga agacgaaatc atcatcgcca aaaaaatcga
    22501 aaacgccctg aaaaatatgg ttcaggccat ctccgcctgc ccgggttcca ttgcggaaat
    22561 cttagaactc atcgaaaaaa tccgcaaaga cgaaatccgc gtcgacgaag tcgtagaagc
    22621 cattatcgac ccgaatgaag tattgctcaa cgaattgggc ttggggcact tggaaaccac
    22681 agcacccgag aaaccttcca acgacaattc ggacgaaaac gaagacgacg aagaatcgga
    22741 agaagatgcg gatgaaatct cggcagccaa tctcgccgaa ttgaaacaaa aagtcatcgg
    22801 ccactttgcc caaatcgaaa aagactacaa aaaaatgatc ggtcgtttgg aaaaacacca
    22861 cagccggcac aaagactatc tcgcctaccg cgacgcgatt gccaacaaac tgctggaagt
    22921 ccgtttcgcc acccggcaaa tcgacagcct cagcagcagc ctgcgcggaa aagtagaaaa
    22981 catccgcaaa ctcgaacgcg aaatccgcga catctgcctc gaccgcgtcc atatggagcg
    23041 cgactacttc atccaaaact tcctgcccga aatcaccaat ctggaatgga ttgaagaaga
    23101 aatcgccaaa ggcagggttt ggagcgacgc gctcgaccgc ttccgccacg ccatcctcga
    23161 aaaacaaacc gagttggcag atatggaaaa agaaacccgc atttccatcg aagagttgaa
    23221 agaaatcaac aaaaatatgg tgtcgagcga aaaagaaacc gcagccgcca aacaggaaat
    23281 gattcaggca aacttgcgcc tcgtcatttc cattgccaaa aaatacacca accgtggctt
    23341 gcagttcctt gatttgattc aggaaggtaa catcggcctg atgaaagcgg tcgacaagtt
    23401 cgaataccgc cggggctaca aattctccac ctacgcaacc tggtggatcc gccaggcaat
    23461 cacccgttcg attgccgatc aggcgcgtac catccgcatt ccggtacata tgattgaaac
    23521 catcaacaag atgaaccgca tctcgcgcca acaccttcaa gaaaccggcg aggaacccga
    23581 ttccgccaaa cttgccgaac tgatgcagat gcccgaagac aaaatccgca aaatcatgaa
    23641 aatcgccaaa gagccgattt cgatggaaac ccccatcggc gacgacgacg attcgcactt
    23701 gggcgacttc atcgaagatg ccaacaatgt tgcgccggcc gatgcggcaa tgtacaccag
    23761 cctgcacgaa gtaaccaaag aaatactcga aagcctgacc ccgcgtgagg caaaagtcct
    23821 gcgtatgcgt ttcggcatcg atatgaacac cgaccacacg ctggaagaag tcggcagaca
    23881 gtttgacgta acgcgcgaac gcatccgaca aatcgaggca aaagcactcc gcaagctgcg
    23941 gcatccgaca agaagcgacc gtttgagaag tttcttggac agcgaagaca gcaagctgta
    24001 aaccaaaaaa ccgcaggttt caaatacctg cggttttttc ttacacaata aacaacgctt
    24061 ccacatatcc cacactccta tcc
//
LOCUS       DAWFBO010000010        23639 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFBO010000010 DAWFBO010000000
VERSION     DAWFBO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23639
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.010"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(67..906)
                     /locus_tag="WGO87_000293"
     CDS             complement(67..906)
                     /locus_tag="WGO87_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0391715.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1191..1266
                     /locus_tag="WGO87_000294"
     tRNA            1191..1266
                     /locus_tag="WGO87_000294"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1224..1226,aa:Arg,seq:ccg)
     gene            complement(1331..2524)
                     /locus_tag="WGO87_000295"
     CDS             complement(1331..2524)
                     /locus_tag="WGO87_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0391716.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAATPVPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFESAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2530..3633)
                     /gene="trmA"
                     /locus_tag="WGO87_000296"
     CDS             complement(2530..3633)
                     /gene="trmA"
                     /locus_tag="WGO87_000296"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0391717.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     TVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            3776..4876
                     /gene="aroC"
                     /locus_tag="WGO87_000297"
     CDS             3776..4876
                     /gene="aroC"
                     /locus_tag="WGO87_000297"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0391718.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRRETDQVEILSGVFKGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4967..5392
                     /locus_tag="WGO87_000298"
     CDS             4967..5392
                     /locus_tag="WGO87_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0391719.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5466..7451)
                     /gene="parE"
                     /locus_tag="WGO87_000299"
     CDS             complement(5466..7451)
                     /gene="parE"
                     /locus_tag="WGO87_000299"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0391720.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7518..8042)
                     /locus_tag="WGO87_000300"
     CDS             complement(7518..8042)
                     /locus_tag="WGO87_000300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0391721.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8207..9502
                     /gene="serS"
                     /locus_tag="WGO87_000301"
     CDS             8207..9502
                     /gene="serS"
                     /locus_tag="WGO87_000301"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0391722.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYEFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9575..10573)
                     /locus_tag="WGO87_000302"
     CDS             complement(9575..10573)
                     /locus_tag="WGO87_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0391723.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10698..11774)
                     /gene="prfA"
                     /locus_tag="WGO87_000303"
     CDS             complement(10698..11774)
                     /gene="prfA"
                     /locus_tag="WGO87_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0391724.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDTDDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11864..12634)
                     /locus_tag="WGO87_000304"
     CDS             complement(11864..12634)
                     /locus_tag="WGO87_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0391725.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(12749..13738)
                     /locus_tag="WGO87_000305"
     CDS             complement(12749..13738)
                     /locus_tag="WGO87_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0391726.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     VKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGRLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13805..14488)
                     /locus_tag="WGO87_000306"
     CDS             complement(13805..14488)
                     /locus_tag="WGO87_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0391727.1"
                     /translation="MFAFLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14679..16013)
                     /gene="glmM"
                     /locus_tag="WGO87_000307"
     CDS             complement(14679..16013)
                     /gene="glmM"
                     /locus_tag="WGO87_000307"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0391728.1"
                     /translation="MAKKYFGTDGVRSEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVIQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVVDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAKVEKELEGKGRVVLRASGTEPVVRVMVEARQADWAREGAERIASAIGSL"
     gene            complement(16143..17000)
                     /gene="folP"
                     /locus_tag="WGO87_000308"
     CDS             complement(16143..17000)
                     /gene="folP"
                     /locus_tag="WGO87_000308"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0391729.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADYVSPEEEWARVEPVLAEVAGWGVPISLDTRR
                     TVIMEKALALGGVDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     YVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKPLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(17140..18033)
                     /locus_tag="WGO87_000309"
     CDS             complement(17140..18033)
                     /locus_tag="WGO87_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ0391730.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18124..20238
                     /locus_tag="WGO87_000310"
     CDS             18124..20238
                     /locus_tag="WGO87_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0391731.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20303..21781)
                     /gene="ubiD"
                     /locus_tag="WGO87_000311"
     CDS             complement(20303..21781)
                     /gene="ubiD"
                     /locus_tag="WGO87_000311"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0391732.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVIGMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21801..22316)
                     /locus_tag="WGO87_000312"
     CDS             complement(21801..22316)
                     /locus_tag="WGO87_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0391733.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNWLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22323..22853)
                     /locus_tag="WGO87_000313"
     CDS             complement(22323..22853)
                     /locus_tag="WGO87_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391734.1"
                     /translation="MVRQGEATLYWFLLIHYHYGREAGWFVWLAAAMCGLWLLRALTA
                     KLPQQRYAALFITLFFAAVLVFGRRDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLA
                     RLQQPDLPEKAVRYTRRVTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMG
                     MPFAGEWLYRKLVLKV"
     gene            complement(23056..23625)
                     /locus_tag="WGO87_000314"
                     /pseudo
     CDS             complement(23056..23625)
                     /locus_tag="WGO87_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
BASE COUNT         5395 a         6735 c         6154 g         5355 t
ORIGIN      
        1 attcccataa aaaaacggag cagatacctg ccccgttttt atttaatccg aaattttaat
       61 ctaaatttag aattttgcac cggattggtt tgccatatag tcaacagccg ctttgacttc
      121 gtcatcgctc aaaccggcat tgccgccttt ggccggcatc gcgttaaagc cttcaagggc
      181 gtgtttgtgc aaggtttctt tgcctttttt gatacgcggt gcccaatcgt cttttttgcc
      241 tatgccggga ataccgggaa tcgaaccgcc gtggcacacc tgacaggttg cttcgaagac
      301 ttttttaccg tcaacgccga ccgcaggggc tgccgcaccc ttgtcttctg ccttcgcttc
      361 tgccggagct gcactatcgg caggagcaga agctgttcct gaagcggcat tgtcggcagg
      421 cgcagcctca tcaggattcg ggaaagaacc gccgcttttg ttcgccatgt aagtaatcgc
      481 ccgtttgagt tcctgatcgg tcaggtctgc cgcaccgcct ttggcaggca tggcgttaaa
      541 gccgttcagc gcgtgttgga acaaggtatc gaagccttgc gcgatacgcg gtgcccaatc
      601 gccgttgtgt tccagtttcg gagcgttcgg cacattgctg tccgccgcgt ggcattggat
      661 acagattttg ccgaaaatct gttcgccttg gcgttcgccg acggggatgc cgtcgcccat
      721 cgtcaattgt ccgacaggct ggatacgggt ctgcgttgct gcttccgtag tggcatcgac
      781 atcgccgaac gagccgctgc ccgccagctt aatcaggaaa taaaggactg caataacaat
      841 aacgataccg ctcacaaggg taaacagtgc agagccttgg gctttgttgt cgcggagttg
      901 tttcatttgg taggcctcgc cgtcaggtta ggttgtgctg taaattatag tttggtgtgt
      961 taaacgcagt taacaatatt ttgctggatt atactgaatt cacagggtct ttccaatcgc
     1021 tatcattgaa aatatgaaaa aatttgccaa cggtatctgt ataaaacaaa taatcctttg
     1081 aaaataatgg tttatcctca agaaaactct ccttatgccg ccatacgccg cctgccggcg
     1141 caagataacc tttgccaatt tgcagaattt acgttaacct tacgttttac gcacccatag
     1201 ctcagttgga agagtgtcag tttccgaagc tggaggtcac aggttcgatc cctgttgggt
     1261 gcgccaatta taaagagacc gtctgaaaga taaatatttt tcagacggtc ttttgactta
     1321 cttcaaactc ttatttcaag acttccgcaa atgcgcgggc aacatagtcg gtattcgacg
     1381 tattcagtcc ggcgacgcac atcctgccgg aatccagcag gtaaacggca aattcgtcgc
     1441 gcagcctgcg gacttgttcc acgctcaatc ctgtgtagcc gaacatgccg cgctgtttga
     1501 tgaaataagt gaaatcgcga tcggggattt gcgcagttaa gacatcataa agtttctgcc
     1561 gcatcgcacg gatgcggtcg cgcatcatat aaacctcgtt ttgccacaag gcgtaaagtt
     1621 cggggctgtt catcacgtcg gcggcgatat acgcgccgtg cgcgggcggg ctggagtaga
     1681 tgcggcggac ggtgaatttg agctgtccga acaccaaatc cgcttcttcc ttattcgggc
     1741 aaaccacgct caggccgccg acgcgctcgc cgtagagcga caggtttttt gagaaggaat
     1801 tgctgacgaa caagggcaat tccatttcca ccgctttgcg gacggcgtag gcatcgctgt
     1861 ccaaatcgcc gccgaagcct tggtaggcga tgtccataaa cggaatcagt ttgcgcgttt
     1921 tgatgatgtg caacacttcg tcccattgtt gttccgacat atccacgccg gtcgggttgt
     1981 ggcagcaggg atggaggatc aggacgctgt tttcgggcag ggtgttgaaa aacgcggtca
     2041 tttcgtcgaa tttcacgccg acggcagcag ggtcgtaata tgggtaagtg ccgacctcga
     2101 aacctgcgct ttcaaaaatg ccgcgatggt tgtcccaagt cgggtcgctg acgtaggcgc
     2161 gcgcttcggg aaaccagcgg tgcagaaagt ccgccccgac tttgagcgcg cctgagccgc
     2221 ccaaggtttg tacggtaaca atacgtcctt gcgcgagcgc ggggttgtct ttgccgaaca
     2281 acaaatgctg caccgcgctg cggtaagtgt ctaagccttc catcggcagg tagggcgacg
     2341 gcacaggagt ggcggcgcgg gcggtttcgg cgcggctcac ggattccaac acgggcattt
     2401 tgccttcgtc gtcgaaataa atgcctatgc tcaaattgac tttttcgggg cgcgggtcgt
     2461 ttttgaaggt ttcgaccaaa ctcaaaatcg gatcgccggg atagtattcg atgtgtcggt
     2521 acatagtcct tacctcttgc tttttccaag gattttcttt ttcaacagca caccactttc
     2581 aatatggtgc gtaaacggaa attgatcgaa cagggcggca cgttcaaccg tatgggtttc
     2641 cgccaaggtg tccaaattgg tacgcagcgt ttcgggattg caggaaatgt agatgatgtt
     2701 gtcaaactgc gacaccagct tcaaagtttc ctcatcgata ccggcacgcg gcggatcgac
     2761 gaaaatagtg gaaaatgcgt aatccgtcaa agcaataccg ccatccttaa ggcgtttaaa
     2821 ctcacgtttt ccggtatagg cttcggtaaa ttcttcagca gacagacggg cgattttgat
     2881 gttgccgatg cggttggctt cgatattcca ttgcgccgcg ctgacggagg ttttggagat
     2941 ttcggttgcc aaaacacgtt caaacttttc agataaagga agcgtaaagt tgccgtttcc
     3001 gcaatacagt tcgagcaaat ccccatccaa tccttctgct gcaccgcagg cccattcaag
     3061 cattttctga cacacggcgg cattcggttg ggtaaaactg ccttcaattt gccgataacg
     3121 gaaatcccgg ttgccgacct tcaaagtttc cgttacatag tcctgtttta agactatttt
     3181 ctgtcccctg ctccgcccga tgacggaaat atccaactgt tgctgtaacg cttgcgccgc
     3241 ctgcatccac tcattatcaa gccttttgtg gtaaatcatg gtaaccagca tttccccgct
     3301 gagcgtagac aaaaattcga cggcatacca acggtttctc agttcggcag attgggcggc
     3361 aacggcaatc aattctggca tcaggcggtt gacggcttcg gaagccgctt caaaacggtc
     3421 gcaacggatc aggcttgccc cgccagcctt ttgtcctttt tcaaacatgg cataaaacat
     3481 ttcaccgcct tcgtgccaaa tacggaactc ggcacgcata cggtaatgtt tgtccggaga
     3541 ttcgtacact tcccactcag gaacatccaa acctgcaaaa agggttttaa ggtggtcttt
     3601 tttatcttga agctgctgcg tgtagtcgct catatcgcta tcctgaaaaa tcagaccgga
     3661 ttataagccg attcactgca aaacacgcaa cccctttacc ggcatataac ccgttttcct
     3721 ccaactttta tgctaaaatc cgccgtctcc aacataccgc cgacagaagg aaaatatggc
     3781 aggcaacact ttcggacaac tcttcaccgt gaccacgttc ggcgaaagcc acggcgcagg
     3841 tttgggctgc atcatcgacg gctgcccgcc cggcttggaa ttaagcgaag cggatatcca
     3901 atttgacctc gaccgacgca aacccggcac cagccgccac gttacccaac gccgcgaaac
     3961 cgaccaagta gaaatcctct ccggcgtatt caaaggcaaa accaccggca cgcccatcgc
     4021 cctcttaatc cgcaataccg accagcgcag caaagactac ggcaacatcg cccaaagttt
     4081 ccgccccggc catgccgact atacctattg gcacaaatac ggcacgcgcg actaccgcgg
     4141 cggcggcagg agctccgccc gtgaaaccgc cgcccgcgtt gctgccggag ccgttgccaa
     4201 aaaatggttg aaagaaaaat tcggcacgga aatcaccgcc tacgttaccc aagtcggcga
     4261 aaaagaaatc cggtttgaag gctgcgaaca catttcccaa aatccttttt ttgccgccaa
     4321 ccatagccaa attgccgagc tggaaaacta tatggacagc gtgcgcaaat cgttggattc
     4381 cgtcggcgcg aagctgcata ttgaagccgc caatgtcccc gtcggcttgg gcgaacccgt
     4441 tttcgaccgc ctcgatgccg aaatcgccta cgcgatgatg ggcatcaacg ccgtaaaagg
     4501 cgtggaaatc ggcgcaggtt ttgacagcgt aacgcaacgc ggcagcgaac acggcgacga
     4561 actgaccccg caaggcttcc tgtccaacca ttcgggcggc atcctcggcg gcatcagtac
     4621 cgggcaagac atctgcgtca atatcgccat caaacccacc agctccatcg ccaccccgcg
     4681 ccgcagtatc gacatcaacg gcaaccccat cgaactcgcc acgcacggca ggcacgaccc
     4741 ctgcgtcggc ttgcgcgccg cgccgattgc cgaagctatg ctcgccttgg ttttaatcga
     4801 ccacgccctg cgccatcgtg cgcaaaatgc ggatgttgcc gtggatacgc ccgatatcgc
     4861 ccgctcggac aagtaaaaag atttagccaa aacacgggct ttatactaaa ataccgacca
     4921 ttgccgtgcc gtctgaacgc acgattctta gaggaataca accgaaatga cacaagaaac
     4981 cgctttgggt gcggcactga aatccgccgt ccaaactatg agcaaaaaga aacagaccga
     5041 aatgatcgcc gaccacatct acggcaaata cgatgtgttc aaacgcttca aaccgttggc
     5101 actcggcatc gatcaggatt tgattgccgc tttgccgcaa tacgatgccg cactgattgc
     5161 acgcgtcctc gccaaccact gccgccgtcc gcgctatctg aaagccttgg cgcgcggcgg
     5221 caaacgtttc gatttgaaca accgtttcaa aggcgaagtt acccccgaag aacaggcgat
     5281 cgcgcaaaac catcctttcg tgcagcaggc tttgcaacag cagtccgccc aagctgccgc
     5341 cgaaacgccg tctgttgaag ccgaagcagc cgaatcttcc gcagcagaat aaatccccaa
     5401 atgaaatgcc gtctgaaaac cgatttggtt tcagacggca tttttccgta tgcggcaatc
     5461 acggttcaaa tatcgagttc cgccgtatcg ccttcgcgct ccatccaggc acggcgggcg
     5521 gcggcttcgc ctttgcccat cagtttgacg aagatgtcgc gcgtctcgtc atccgcgcct
     5581 tcggggattt gtacctgcaa caggcggcgg gtgtcggggt gcatggtagt atctttgagc
     5641 tggtctgggt tcatctcacc caaacctttg aaacggctga tggaataagc agtttcttta
     5701 acgccttctt tttgcagccg ctctaaaatg ccgtcaagtt cgttttggtc gagggcgtag
     5761 aatttgcggg cgggtttgct tttaccttgt gcgttgacat ctacgcggaa cagcggcggc
     5821 tgggcgacgt agatgtgtcc gtcagcgacc agtttcggga agtggcggta gaacagggtc
     5881 agcagcaaaa cttgaatatg cgagccgtcc acgtcggcat cggacaggat ggcgattttg
     5941 ccgtaacgca ggccgcttaa atcgggatgg tcgttgatgc cgtgcggatc gacgccgatg
     6001 gcaactgaaa tgtcgtggat ttcggcgttg ccgaagagct ggtcggggtg gacttcaaag
     6061 ctgttgagca ctttgccgcg caggggcagg atggcttggg tggctttgtc gcgggcgagt
     6121 ttggcggagc cgccggcgga atcgccttcg acgaggaaga gttcgttttc gcggatgtct
     6181 tcgctttcgc agtcggtcag cttaccgggc aggacggcga cgccgctgcc ttttttcttt
     6241 tcaatctttt taaccgaacg catccgcgcc tgtgcctgac ggatggcgag ttcggcgatt
     6301 tttttgccga agtccacgtt ttggttcagc cacaattcca aagggtcgcc cgatacggta
     6361 gcaacgagtt tcagcgcgtc gcggttggtc agtttgtctt tggtctgacc ttggaactgc
     6421 gggtcgagga cacgggcaga gaggacaaag gcggttttgc cgaacacgtc gtcgctttgc
     6481 actttcacgc cgcgtggtaa aaggttgtgc agattgatga agttgctgac ggcgttgaac
     6541 acggcttgtt tcaagcccgc ttcgtgcgtg ccgcccagcg gggtggggat gaggttgacg
     6601 tagctttcgt tggcgcacga gccttcttcc agccaggtca gggcaaacgc ggcgccttcg
     6661 ccgatgctga aatcgccgtt gtggccgtct gaaatgtagt tttcgcaaga gaagataggc
     6721 acggcttcct gcgcgtcggc aatcaggtcg gtcagatagc ttttcagacc gtcggggtaa
     6781 tgccaggttt gggtgtgcgc ttcgtcttcg cctttgaccg gacgggtcag ggaaacgcgc
     6841 acgcccggca gcagcacggc tttggcgcgc agcaggcgtt cgagttcggg aatgctgtaa
     6901 ttcgggcttt caaaatattt gccgtccggc cacacgcgca cttcggtgcc gctgtctttg
     6961 acggcgcatt tgcccacttg tgccaacggt tcgaccacgt cgccgccggc aaacacgata
     7021 cggtggattt tgccttcgcg tttgaccgtt acctcaaggc gggtggaaag ggcgttggtc
     7081 acggatacgc ccacgccgtg caggccgcct gaaaaggcat acgcgctgcc gccgtctttt
     7141 ttgttgaact tgccgcctgc gtgcagacgg gtgaacacga gttcgactac gggtacgcct
     7201 tcttcagggt gcagaccgac gggaataccg cggccgttgt cgcgtacgga aagcgaaccg
     7261 tcttcatgaa tttgcacgtc gattgccgta gcgaaaccgc ccaacgcctc gtccgccgcg
     7321 ttatcaatca cttcctgaca gatatgggtc gggctgtcgg tgcgggtgta cataccggga
     7381 cgttctttga ccggttccaa acctttgagg acggtgatgc tggattcgct gtattggttg
     7441 tttttagcca taggaataat ctgaaagtaa gaaaaacaac gctttcagac ggcctgaaag
     7501 cgttgcgttc cgttgtttta ccggttgccg gaaggttggc gggcggcaaa gtcttcataa
     7561 ctttccatac cgcgcaggaa gcgggaagag agttctttca acgcccccaa ataaacgtcg
     7621 cgtttgaact cgatgacttc gtcaacgggc gcccaatatt gatgccaacg ccagccgtca
     7681 aattcggggt ggcgggtggc gcgcaggttg acatcgcaat cccggccggt caggcgcagg
     7741 agataccaaa tctgcttctg cccgcgataa gatccgcgcc attcgcggcg cacccagttg
     7801 ttcggcacgt cgtaacgcag ccagtcgcgc gtccgcccga cgattttgac gtgttgcggc
     7861 agaagtccga cttcttcgta aagctcgcgg tacatcgcgg tttcggggct ttcgcccggc
     7921 ttgatgccgc cttgcggaaa ctgccaagaa tgttcgcgca cgcgcttgcc ccaaaagact
     7981 tcgttacgtt cgttaatcag gataataccg acattggggc gatagccttc cctgtccaac
     8041 acggtgtcgc cctccgttaa attcaatcgt acgattttcc cacaaatcgg gcggttttga
     8101 caaatcagac ggcatggttc aagcatgtcg aaacacagac ggactcggga aaatatctta
     8161 aatttggttt acaataatgt gtttcaaatt attcgggaat cagaccatgt tagatatcca
     8221 attgctccgc agcaacaccg ccgctgtggc ggaacgcttg gcggcgcgcg gttacgagtt
     8281 tgatgccgcg cgtttcaatg cgctggaaga acaacgcaaa gccgtccaag tgaaaaccga
     8341 agaactacaa gcctcgcgta acagcatttc caagcaaatc ggcgcactga aaggtcaggg
     8401 caaacacgaa gaggcgcagg cggccatgga tcaagtcgcc caaatcaaaa ccgatttgga
     8461 acaggctgcc gccgatttgg atgccgttca aaaagaattg gacgcatggt tgttgagcat
     8521 acctaacctg ccgcacgaaa gcgtacctgc cggtaaagac gaaaccgaaa acgtcgaagt
     8581 ccgcaaagtc ggcaccccgc gcgaatttga ctttgaaatc aaagaccatg tcgatttggg
     8641 cgaacctttg ggtttggatt ttgaaggcgg tgcaaaactc tccggcgcac gatttaccgt
     8701 gatgcgcgga caaatcgccc gtctgcaccg cgccttggca cagttcatgc tggatacgca
     8761 cacgctgcaa cacggctaca ccgagcatta cacgccttat atcgttgacg atacgacgct
     8821 gcaaggtacg ggccaactac caaaatttgc ggaagatctg ttccacgtta cccgtggcgg
     8881 cgacgaaacc aaaaccaccc aatacctgat tccgacagcc gaagttaccc tgaccaatac
     8941 cgttgccgac agcattatcc cgtccgaaca actgccgctg aagctgaccg cgcattcgcc
     9001 ctgtttccgc agcgaggcgg gttcgtacgg caaagacacg cgcggtctga ttcgccagca
     9061 ccagttcgac aaagtggaaa tggttcaaat cgttcatccc gaaaaatcat acgaaacgct
     9121 ggaagaaatg gtcggccatg ccgaaaacat cctgaaggct ttggaactgc cctaccgcgt
     9181 gattaccctg tgtaccggcg acatgggctt cggcgcggca aaaacgtatg acttggaagt
     9241 ttgggtgccg gcgcaaaata cctaccgcga aatctcaagc tgctccaact gcgaagattt
     9301 ccaagcccgt cgcatgaagg cgcgtttcaa agacgaaaac ggcaaaaacc gcttggtaca
     9361 tactttgaac ggctccggtt tggcagtcgg cagaaccttg gtcgccgtgt tggaaaacca
     9421 tcaaaacgcc gacggcagca tcaacatccc tgccgcactg caaccgtata tgggcggcgt
     9481 tgccaagttg gaagtgaaat aagccttaac cctgaacata aatgccgtct gaaacttggt
     9541 tcagacggca tttcctttaa acttttaaaa cacgtcagcc gtcggcacga accgcattgc
     9601 cgcaatcgcc ggtctgtccg acctcgcgga tattggacag cgtaacttcc gaaatattac
     9661 ccaacgcctc ttccgtcaaa aatgcctgat ggccggtaaa cagcacatta tgacaagacg
     9721 acaggcggcg gaacacgtcg tcggtaatca catcgttgga tttgtcttca aaaaacagct
     9781 cgcgctcgtt ctcgtacaca tccatgccca atgcgccgat tttccggcgt ttcaacgcct
     9841 caatcgcggc ggcactgtca atcagcccgc cccggctggt gttgataatc atcacgccgt
     9901 ctttcatttt gtcgaacgcc gcttcgttca gcatatagtg gttttccggc gtggaggggc
     9961 aatgcagcgt gatgatgtcc gactgggcat acagctcgtc caaatccaca tattttccgc
    10021 cgagtttttc cacttcgggg ttgcaaaacg gatcgtaagc cagcaggttc ataccgaaac
    10081 cctttaaaat ccgcatcgtt gcgataccga ttttccccgt gccgataacg cccgccgttt
    10141 tgccgtacat attgaaaccg gtcagacctt ccagcgaaaa attcgcatcg cgggtacgct
    10201 gataggcttt gtggatacgg cggttcaacg tcagcatcag accgaccgta tgttccgcaa
    10261 ccgattcggg cgaataggca ggcacgcgca cgactttcaa gcccaactct tcagccgcct
    10321 ttaaatccac attattgaag ccggcacaac gcaacgccac agttttcacg ccaatttgcg
    10381 ccaatttttc caacacgggc cggctgccgt cgtcgttcac aaaaatacag accgcctccg
    10441 caccttccgc cattttcgcc gttttcgcat ccagcataaa atcaaaaaac tccagctcga
    10501 agccgaaatg ccggttggcg cgggtaaaat gttcgcggtc atagcttttc gtaccgtaaa
    10561 tcgcaatctt catcaatatg tccagttgtc gtctatggtt gagaaacggc ataatacact
    10621 gaattcaaac aaatcagtag aatatggtgg attaaaattg attgcatgca cggcatttcc
    10681 atttcaaaac acaaaactca atcgcccatt gccgccagaa gctccgcctg atgctcggca
    10741 atcagggcat cggtgatttc tgccaaatcg ccgtccatca caaaatccag cttgtgcagg
    10801 gtgaggttga tgcggtggtc tgttaccctg ccttggggat agttgtaggt gcggatgcgt
    10861 tcgctgcggt ctccgctgcc gatgagggat ttgcgttcgg cggcttcttt ggcttgggct
    10921 tcgcgttttt gcgcgtcgtt caggcgggcg gcgaggactt tcatcgcctg cgctttgttg
    10981 gcgtgttggc tgcgtccgtc ttggcattcg accaccatgc ctgtgggcag gtgggtgatg
    11041 cggacggcgg aatcggtttt attgatgtgc tgcccgcccg cgccggatgc gcgaaaggtg
    11101 tcgatgcgaa ggtcgacggg gttcaactcg atgtcttcca gttcgtccgc ttcgggcatg
    11161 acggcaacgg tgcaggcgga ggtgtggatg cggccttggc tttcggtggc ggggacgcgc
    11221 tgcacgcggt gtccgcccga ttcaaatttc aaacggctgt acgcgcctaa gccgataata
    11281 cgggcgataa cttctttata gccgcccaac tcgctttcgt tggcggacac gatttcaacc
    11341 tgccagcggt tgcgttcggc gtagcggctg tacatacgca gcaaatcgcc ggcaaacagc
    11401 gcggcttcgt cgccgcccgt tccggcgcgt atttcgatga agatgttttt gtcgtcgtcg
    11461 gtatctttgg gcagcagcag tttttgcaat tcggtatcga gtgcgccgat tttggctttc
    11521 gccgcttcga tttcttcggc ggcaaagtct ttcatttcgg ggtcggacaa catttcttcg
    11581 gcatccgcca aatcgctttg cgccagttga tagttttgga atacttcgac gacgggggtc
    11641 agttcggcgt gttcgcgcgt gagcttgcgg tagttgtcca tatctgccgt ggcttcgggt
    11701 tgccctaaaa ggtgggtaac ttcttccaac cggtcgccga gttgttgcag tttttctaag
    11761 atagacggct tcataattct tccataacaa acgccgcctg aatttcagac ggcatcaaaa
    11821 cgcggtcatt ataatatatt ttctgaatat tcaaaaagat aatctaggga cggagcaggc
    11881 tgtcggcatt gcgttccgtt atcgcggcga tgtccgccac gccgacaccg cgtatctgcg
    11941 cggcaatttc ggcaatccaa agaatattgg caggcgtatt aatctctttt ttcagcataa
    12001 acgggctgtc ggtttccaat acgaaatccc cgtcgttcaa ggctttaagc gtatcgcgca
    12061 ctttacgcgc gttcggattg agcaacaacg aaccgatgcc gattttgaaa cccagtttcg
    12121 tcaacacgcg cgcttcttcc gcgctgcctg agaaggcgtg aacgatgccg ccttgggcaa
    12181 agcctgtctg tttgacggcg gcggcgatgt cggcggtggc tttgagatta tggataatca
    12241 cgcggcggcg cagggtttgc gcgatggcaa gctggcggac gaaaacttgg atttgccgtt
    12301 cgcgctgcgg cggcgtttgg gttttgtcgt aaaaatccaa gccgatttcg ccgacccatg
    12361 cctgcggata acgtgccaac atcgcttcca ggcggacgca atcacgctcg gcaatgccgt
    12421 ctgaaaacca aggatggatg ccgagcgcaa tacggatttg accgtgttcg gacggcattt
    12481 ccgacagttc cgccacgctt tgccaatcct gcggacaggt ggcgggaacg ataaaccgct
    12541 tcaccccaac tttccgcgcg gcggtcagga tgtgcggcag gttttcgcgc agggcggcat
    12601 cggcaaggtg gcagtgggtg tcggtgaagt tcatttcgat tccgcaatgc ggcaggttca
    12661 cataaaaata aggcagggca gcaagggatg ccgtgtacgg gcggcgcata aggcgcggtc
    12721 aacgccccgt cattgccatt tcaatccgct ataacttcag tcttgccaac tctttttcca
    12781 cctcttcctt accccagcct tgcgatacgg cgagggtcat gagtgcggtg gcggtttcga
    12841 ggttgcattt gccgccgttg atgatgcctg agttgcggaa cgcgttgcct tgcgcgtaaa
    12901 cggcagcggt tctgccttgt cggacttggc tgatgttgag cagcagcctg ccttgctgcg
    12961 taaagtcttg cacggcgcgg atgaaacctt cgtctgcggg cgtgttgccg tgtccgtaac
    13021 tttgcaggat aagggcgtgg gcgggaagct gtccgagtcc gtcggcaagt tcttggacgg
    13081 caaagccggg aataagcgtg cggacggcga tttttgcctg cgggtcagga tagcggattt
    13141 tgaggccgtc tgaaacggcc gccgcatctt gggacggaat acgaagattg taccagcctt
    13201 gagtttcatc ccattcggcg atgacaccga aatgcggatt gtcgaagcct gcggcggttt
    13261 cggtgctgac tttgctgctg ccgacggcgg gataaagttt gccgtcaaag gcaataagcg
    13321 tctgcttcaa tccgagtggg aatgcggcaa cggcagtcgc cagattgcgc ggcgcgtcgc
    13381 tgcccacggc gttgtacggc cattgggaac cggtcaggac aatcggtttg accaaaccct
    13441 gcagcgcgag ggcgaacaga ttggcggtat aggcgagcgt gtccgtaccg tgcagcacca
    13501 aaacgccgtc gtactgcggc agcgcggcgg caatgatgtc cagccaagcg caccagtttt
    13561 gcagttggac ggcggaagaa tcaatcagcg tcccacaaac gtgccaatcg aaatccatgc
    13621 cgtttgaaaa cggcgcgagt gcctgcccga ccagcgcggt atcggggcgc aggccttcgc
    13681 tgctttgcgt catgccgatg gtgccgccgg tataaaggac aaaaattttc tgtttcatgg
    13741 ctcatcctga aaatgcaaag accgcctgaa acgcgaaatg cgtccggacg gtcgaaaatc
    13801 tgatttactg ctgcttctgt gccgcttttt tcgctgcctt ttccgccttg cgtttggcgc
    13861 gtttttcgct caattgtgcg cggtaaagct gataatgtcg gcgtttgcgc caccagaacc
    13921 aagccagcgc cgccgccagc acgcccaatg cgatgaagat gccggattgc aggctgtgca
    13981 ttttcgccat cagccaatcg atgttgtgcg cgccgtactc gcccaagtaa atccaaacgg
    14041 gcacggaaat cagcgcggca agcccgtcca taatcagaaa gcgcagatac gatactttgc
    14101 ggctgatgcc ggcggtaacg aaaacggcag tccgcaaacc cggcaggaag cgggcgacaa
    14161 acagaaccca gttgccgtat ttgtcgaatt tttcctgaac ctgtgcgtaa cgtttcggcg
    14221 tcatgatgcg cgcaatcggt ttgaacttga ggattttctg cccccaaatt cgtccggcgg
    14281 cgaacatgat gccgtcgccc gccaacacgc cgagcatacc gactgcaaac ataatatgcg
    14341 gattggtata acccataccc gaaatcacgc cgcctgttac caaggtcaaa tcctcgggaa
    14401 tcggcacgcc gaaaccgcag atgaccaata caaaaaaaac agccgcataa ccgtattcga
    14461 caaaaaaggc ttctaaaaaa gcaaacatgg cggatattcc attgtcggag ataaaaagtc
    14521 agaacaaacc gaaacatttt ctacatgaag caggcattct atcaaagatt atgccgtctg
    14581 aaagcggaaa aaaggcagat tcgcatatcc tccgcatgtt cagacggcat ttaaacagaa
    14641 aaccggccgg ttctgaaaac ggtttttcct gattttgcct aaaggctgcc gatggcggaa
    14701 gcaatacgtt ccgccccttc gcgcgcccaa tcggcctgtc gtgcctcaac catcacacgc
    14761 acgacgggtt cagttcccga agcgcgcaac acaacccttc ctttgccttc gagttctttt
    14821 tccactttgg ccaacacgtc tttcgaagcc tcctgccatt tctgaccctt ttggatgcgc
    14881 acgttaatca tagtttgcgg atacggctgc cagtcggcgc aaacggtggc gaggtcttgg
    14941 ttcaggattt gcagcgccgc caaaacttgc agcgcggaaa taatgccgtc gccggtgtta
    15001 tgtttgtcca tgcacaaaat atggccgctg gcttcgccgc cgatgagcca gccgcgttgg
    15061 ttcaactgtt ccaacacata gcggtcgccg actttggcgc ggcagaaatc gacgccctgc
    15121 tctttcaggg cgatttccat cgccatattg gtcatgaccg tgccgaccac gccgccgatg
    15181 ttgatacctt ctcgggcgcg ggctttggcg atgacgtaaa tcaggctgtc gccgtcgtaa
    15241 accttaccgt ttttatcgac catcatcagg cggtcgccgt cgccgtctaa ggcgatgccg
    15301 tagtcggctt catgctgtaa aacggcggcc tggagtgtct tggtataagt cgcaccgcat
    15361 ttctcattga tattgtagcc gttgggttcg tcgccgatgc tgacaacctg tgcgcccagt
    15421 tcgtgaaaca ccttgggtgc aacaccgtac cccgcgccgt tggcggtatc gacaaccaac
    15481 ttcaaacccc gaaggtcgga atggctgggg aaggtggatt tgcaaaattc gatatagcgg
    15541 tcgtccgcac cgctgatgcg gcgggcgcgt ccgaggcgtg cagaaggctg ggttttcatt
    15601 tcttcgtcga ttttggcttc gatttccaac tcgatttcat cgctcagttt cacgccgccc
    15661 tcggcaaaga atttgatgcc gttgtcggaa taggcattat gcgacgcgga aatcatcaca
    15721 ccggcggaca aacgcagcgc acgggtcaga taagccacgc cgggcgtagg cagcggaccg
    15781 gtttggataa cgttgacacc tgcggcggta aaacctgcca ccaaagcagc ttccagcata
    15841 taaccggaaa tacgcgtatc cttgccgatt aaaacggtcg gtttctggtc ggtgtcgtgc
    15901 tgaaccaaaa cctgacccgc cgcatagccg agtttcaata caaaatcggg cgtaatcgga
    15961 aactgcccga cttcgctgcg cacgccgtcc gtgccgaaat atttttttgc catgtgttgc
    16021 tccgagaatg tgaaccgttg tccgagatta tacagtcagt ttgtgccttg ctgtctgcac
    16081 cgttgatgcc gtctgaaacc gccccgtcct tttcagacgg catgaagtat gtgaaccgct
    16141 gtttacagat tgatgcccaa cgcttcccac accttcaacg catccgctgt cgccttcaca
    16201 tcatgcaccc gcacgatttg cgcgccgcgc gctacggaag ccaacgctgc cgccacgctg
    16261 ccgtgtacgc gttccgccgc atttgcctcg ccggtcagct cgcctatcgt gcttttgcgc
    16321 gatacgccga tgagcagcgg aaaacctgtt tccgccatca attcgggcaa atgccgcatc
    16381 agcgcgatat tgtgttgcaa gggtttgccg aagccggagc cgaagccggg gtcgagtatg
    16441 atgcgttgcg gtgcgatgcc tgccgcgata cattccgctg agcgcgcttt caaataccgc
    16501 gctacttcac cgacaacata ttgatatttc ggattaatct gcatggtttt gggcaaaccc
    16561 tgcatgtgca tcaggcaaat gcccgtgtcc gcctgacgcg ccagcaattc gaccgcgccc
    16621 tcgtcattca acgccgccac atcattaata atatcgacgc cgccgagtgc caacgctttt
    16681 tccataatca ccgtgcggcg cgtgtccaaa ctgatgggaa cgccccaccc cgccacttcc
    16741 gccaaaacag gctcaacccg cgcccattct tcttcaggcg aaacataatc cgcacccgac
    16801 cgcgtcgatt cgccgccgat gtcgagaatg tccgcaccct ctttcaaaag ccgttcggcg
    16861 tgcgccaaag ctgtttgggc attttgcgaa tacgcgccgc cgtcggaaaa agaatcgggc
    16921 gtgagattca cgatacccat gattttcggt ttgtccaaac cgatttcaaa ccgtcctgcc
    16981 tgccaaacgt gtcgtgccat ctgaactcct cccaaaataa aaaacagatt atatgccgtc
    17041 tgaaaccgtc ttgtgcgctt cagacggcac cgctattcag gcggcagacg gcatgttgtc
    17101 cgaatgtctg ctccgccttt gaatctgccg gtatgcctgc tatccgcccg acttttcaaa
    17161 acaggttccg acgattccgc acgcgcctgc cgcctttgcc aagccgtaca ggatttcctg
    17221 cggcatatcg cggttccata atcccgtaat attcgcaatc acgggcagat ggctgatttg
    17281 gcggactttc acgatggatt cgacatccaa acggtaggga tggtctttgg tatggctcaa
    17341 cacgcccgac tcgcccagaa tcaggtggcg gttgccgcgc gagacaacat attctgcggc
    17401 attcaaccaa tcttcggcag aatggtgctt gtctttacac agcaccaagg gaatgttcag
    17461 gcttccggct tcattcaata cggcaagatc ggacatcagc ccgccgccca aatacaggat
    17521 gtccgccccc gcattcaaag ccgcttcgac atggcggacg ttgcggacgc gcaccaatac
    17581 gggtttccct gcgccatgcg ccgatgcggt ctgttccgcc aaccgtctgc accgtcctcg
    17641 cccttcatcc gcacttgaag tgtcgtataa gtttgccgaa gtgaaaaacg gatctagaaa
    17701 cactgcatcc gcattgcgcc atactgacgg ttctgcggcg atacagacgg tttccccgcc
    17761 gccgaaagcc acgcctttgg cggcaacgcg gctgtcttcc gcccgatttt cccgactgac
    17821 ggttttccat gtatccaaaa tgcggacggc tttctcgacc tccggcagcg tctgcacctc
    17881 cctgacgctc aaaaccctat cgtcgccgat tgcgccgatg acggtacgct cgtcgccgtg
    17941 agaaatgtgt tctcgcagac ctctgctgcg gataaaggcg acaacgccgg caatgtccgc
    18001 ttcggcggca cgcctgctca tgacaataat catatttcct cctgacacaa gaaacggcct
    18061 gcccaaaata ggatttttgc aagccgtgtt atactgtggc gtgttttaca gattgttcgg
    18121 gctatggatt tattatcggt tttccacaaa taccgtctga aatatgcggt ggccgtgctg
    18181 acgatactgc ttttggcggc agtcgggctg cacgcttccg tatatcgcac cttcacgcct
    18241 gaaaacatcc gcagccgcct ccaacaaagc attgcacaca cacaccggaa aatctcgttt
    18301 gatgcggaca ttcagcgcag gctcctgccc cggccgaccg tcatcctgaa aaacctgacc
    18361 attaccgaac ccggcggcga ccagactgcc gtttccgtcc aagaaaccaa aatcggattg
    18421 agctggaaaa acctgtggtc ggatcagata cagattgaaa aatgggtggt ttcgagtgcg
    18481 gaacttgccc tgacgcgcga cgggaaaggt gtttggaaca tccaagacct gatcgacagc
    18541 caaaaacgcc aagcctcagt caaccgcatt atcgtcgaaa acagcaccgt ccgcctcaat
    18601 ttcctgcagg aacagcttat cctgaaggaa atcaacctca acctgcaatc ccccgattcg
    18661 tcggggcagc cgtttgaaag ttcgggcata ctggtttggg gaaagctgtc cgtcccgtgg
    18721 aaaagcaggg ggctgttcct ttcagacggc atcggcacgc ccaaaatctc accgttccat
    18781 tttgaagctt ccacttcgct ggacggacac ggcattacca tttccaccac cggcagccct
    18841 tctgtccgct tcaacgccgg cggagcggat gccgccggcc tcggcctgcg tgcagacact
    18901 tccttccgca acctccacct gaccgcccaa atccctacgc tggcactcag aaataacagc
    18961 attaaaattg aaaccgtcaa cggcgcattt accgccggcg gcgaatatgc ccaatgggac
    19021 ggttcgttca aactcgacaa agccaacctt cactccggca tcgccaacat cggcaacgcc
    19081 gaaatctccg gcagcttcaa aacaccgcgc caccagacca acttctccct caattcgccg
    19141 ctcgtatgga cggaaaacaa agggctggac gcgccgcgcc tgtatgtatc gacccttcag
    19201 gataccgtca accgcctgcc gcaaccccgt ttcatcagcc ggctcgacgg ttcgctgtcc
    19261 gtaccgaatc tgcaaaattg gaatgccgaa ttaaacggca cattcgaccg ccaaaccgtt
    19321 gccgcgaaat tcagatacac acatgaagac gcaccgcatc tggaagccgc cgtcgcactg
    19381 caaaaattga acctgacccc ctatcttgac gacgtgcggc aacaaaacgg caaaatattt
    19441 cccgacaccc tcgccaagct gtccggcgac atcgaggcgc acctgaaaat cggaaaagtc
    19501 caacttcccg gcctgcaact ggacgatatg gaaacctacc tccacgccga caaaggccat
    19561 atcgcgctca gccgtttcaa gtcagggctt tacggcggcc ataccgaagg cggcatcagc
    19621 atcgccaaca cccgtcccgc cacttaccgc ctgcaacaga atgcaagcaa catccaaatc
    19681 caaccgctgc tgcaagacct gttcggcttc cacagcttca gcggcaacgg cgatgcggtc
    19741 atcgacctga ccgcgggcgg cgaaacccga aaagagctta tccgctcgct tcagggcagc
    19801 ctgtcgctga atatttccaa cggcgcgtgg cacggtatcg acatggacaa tatcctgaaa
    19861 aacggcattt cgggcaaaac tgccgacaat gccgcaccca gcacaccctt ccaccgattc
    19921 acgctcaaca gcgaaatttc agacggcatc agccgccaca tcgataccga actcttctcc
    19981 gacagcctct atgttaccag caacggctat accaatctgg atacgcagga attgtctgaa
    20041 gatgtcctta tccgcaacgc cgtccatccg aaaaacaaac cgattcccct gaaaatcacc
    20101 ggtacggtgg acaaaccgtc cattaccgtc gattacggca ggctgaccgg cggcatcaat
    20161 tcgcgcaaag agaaacagaa aatcctcgaa gacaccctgc tggaacaatg gcagtggctc
    20221 aaacctaaag aaccgtaaac atcctgcgta caaaaatgcc gtctgaaaca cccccgcgct
    20281 tcagacggca tgaccgtaaa acctacaacc ccaattcctc ccaaatccca tcaatcttag
    20341 ccgtaaccgc agggtctttt ttgatgacgc gcccccattc gcggtcggtt tctcccggcc
    20401 atttgttggt cgcatccaaa cccattttgc cgccgagtcc gctaacgggg ctggcgaagt
    20461 cgagataatc gatgggcgtg ttttctacca aaacagtgtc gcgcacgggg tccatgcgcg
    20521 tggttaccgc ccagatgact tctttccagt cgcgcacgtt cacatcgtca tccaccacga
    20581 tgatgaattt ggtatacata aactggcgca ggaacgacca gcagcccatc atcacgcgct
    20641 tggcgtgtcc ggcgtactgt tttttcatgc cgatcaccgc catgcggtag gagcagcctt
    20701 cgggcggcag gtaaaaatcg gtgatttcgg ggaactgctt ttgcaaaagc ggtacgaaca
    20761 cttcgttcaa cgccacgccc aaaacagcgg gttcatcggg cggtttgcct gtgtaggtcg
    20821 aatggtaaat cgggttttcg cgcatggtga tgcgctcgac cgtaaataca gggaaatgat
    20881 cctgctcgtt gtaatagccg gtgtggtcgc cgtatgggcc ttccaacgcg gtttcgttcg
    20941 gatgaatcac gccttccaat acaatttcag cacgggcagg cacttgcaaa tcgttgccga
    21001 tacatttcac cagctccgtc cgcgaaccgc gcagcagtcc ggcaaactgg tattcgctca
    21061 aagtatcggg aacaggcgtt accgctccga gaatagtggc agggtcgcaa ccgagcacga
    21121 cggcgacggg atacggcgta tcgggattga gtttgcggaa ctcctgataa tccaacgcgc
    21181 cgccgcgatg cgacagccaa cgcataatca gcttgttttt gccgatgagt tgttggcggt
    21241 aaatgccgag attttggcgt tttttgtgcg gcccgcgcgt tacggtcaag ccccatgtta
    21301 ccagcggcgc aacgtcttcc ggccagcaat gctgaatcgg aagtttatat aaatcaacgt
    21361 cttcgccttc ccacacgatt tcctgacacg gcgcgttttt caccacgttc ggcgccatgc
    21421 tccaaatgtc tttcaagagc ggcagtttgg aaaacgcgtc tttgatgcct ttgggcggtt
    21481 cgggttcttt cagatacgcc agcgtctgcc cgatttcgcg cagcttggac acgctgtccg
    21541 cgcccatgcc catcgccaca cgttcgggcg tgccaaacaa attcgccaac acaggataat
    21601 cgtagcgcgt accgtcgggc ttaatcgggt tttcaaacaa caacgccggc ccttcggcgc
    21661 gcaacacgcg gtcggcgatt tcggtcattt ccaaatgcgg ggaaacagga tgcgcgacgc
    21721 gcttgagttt gccctgttgc tcgagcatag cgatgaagtc gcgcaggtct ttgtatttca
    21781 tattcatcct ttttgtcctt ttatcctgag caatccgatt cggataccgc ccctatcctt
    21841 gcctgcgctt cggcatattc tatgccgtga taaaagtcgc gtaccagcgg atgtccgcca
    21901 ctttgatgaa gttgcagcaa agggcgttgg ttgtcggact gggtaacgac attgcagtgc
    21961 agaccgaagg tgtcggtttc ataagggggc agccagttgc agatcatgcc gaaataaacg
    22021 acgttttccg gattgtcgtg aaagtggctt tgatagtcgt taaaactctt ttcactgaca
    22081 gatacccata ccccatattc cagcgtttcc tcatgcccga taatcggaat aagcagcacc
    22141 gcgcggataa aacggtcggt ttggtcggga tagcggatga tgcagaaatc agaatcacat
    22201 tccgcttgat aagcaatgcg ttcttcttca ctgagttgat tatagggata gggggcggta
    22261 aagccgattg cgggcatatc ttcatgtttt tcgccgcagg aagtgcaagt gtacataagg
    22321 tttcagactt tcaaaacgag tttgcggtaa agccattcgc cggcaaacgg catccccatc
    22381 aatacatagg caatcacgcc ggtataaacc gcccaccaat catatcgccc caaccgtgcc
    22441 aacaaagcgg caagcgtccc gttgattata aaaaatacgc accaaacctg cgttacccgg
    22501 cgggtatagc gcacggcttt ttcaggcagg tcgggctgtt gcagccgcgc aagtttttca
    22561 atcaccgtct gcccggcaaa caggcttccc ccgaacaccg ccaacatcat cagattgacg
    22621 aggacgggat accaatacat tgagtcgcgc cgcccgaaca ccaatactgc ggcaaaaaac
    22681 agtgtaataa acaaagccgc ataacgctgt tggggcaatt tggcagtcag ggcgcgcagc
    22741 agccacaacc cgcacatcgc cgccgccagc cagacaaacc agcccgcctc cctgccgtaa
    22801 tgatagtgga ttaacaaaaa ccagtacagc gttgcctcgc cttgccgtac tatctgcggc
    22861 ttcgtcgcct tgtcctgatt tttgttaatc cactatacca caaagcggga taggcaatgc
    22921 ttaatacggt cagaaaaata tgtccgaaaa aaccgggttt cattttgaat ccgcacaaat
    22981 gttttcagac ggcatccgat aaaaacatgc cgtctgaaaa ataattagaa atacccgatt
    23041 agcccgcctg aatcttcaat accgcctgta ccacgtcgtt gacggtgcgg acattgcgga
    23101 aatcttcagc ctgcagcttg cgggctgcca cagccattca cgattgcgat atacccggcg
    23161 gcattcccga ctgccgttca gcagaaagcg cacccattcc aaaccgctcc aatatgcctc
    23221 gggcagcata ccggcttcag acggcatatc gtccgaagca gataaagtca ggctgtaacg
    23281 cgtccctttg gtcagaacca tcgccaaagc ataagcaaac ggcgcgcgcg ttgccgatac
    23341 ggcatatcct tccggcagcg gatcgtccgc cgccaaaacc aaaaccgacc cgcatccctc
    23401 ttccaacagt gatgccgctt ccgccaatgc cgtttccaca ccgtcggcac acacggccaa
    23461 tgccgtctgc tcgctcatat cccgcctcaa tatcgaccat taccccgccg ttgcattgtg
    23521 caccgacaaa ccgaacgaag tcggcgacac ggtttgcgat ttcaacagtt ccaaccacaa
    23581 atcgaaactg cgcgacattt ccccgtcgtg cgaggcataa actatagtgg attaaattt
//
LOCUS       DAWFBO010000011        22547 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFBO010000011 DAWFBO010000000
VERSION     DAWFBO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.011"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..380)
                     /locus_tag="WGO87_000315"
     CDS             complement(<1..380)
                     /locus_tag="WGO87_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391735.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WGO87_000316"
     CDS             complement(438..803)
                     /locus_tag="WGO87_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391736.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1333)
                     /locus_tag="WGO87_000317"
     CDS             complement(785..1333)
                     /locus_tag="WGO87_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391737.1"
                     /translation="MKKQITAAMMILTMIAAPAMANSMDNQIFENQVFHTRADAPMQL
                     AELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIP
                     GGVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPG
                     QGIGRHRPWESKSTDRSWKNRF"
     gene            complement(1496..1767)
                     /locus_tag="WGO87_000318"
                     /pseudo
     CDS             complement(1496..1767)
                     /locus_tag="WGO87_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2329..2802)
                     /locus_tag="WGO87_000319"
     CDS             complement(2329..2802)
                     /locus_tag="WGO87_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391738.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            complement(2890..3114)
                     /locus_tag="WGO87_000320"
     CDS             complement(2890..3114)
                     /locus_tag="WGO87_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391739.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            complement(3385..3672)
                     /locus_tag="WGO87_000321"
     CDS             complement(3385..3672)
                     /locus_tag="WGO87_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391740.1"
                     /translation="MDDLIAAFLGYLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIRKTINKIK"
     gene            complement(3984..4355)
                     /locus_tag="WGO87_000322"
     CDS             complement(3984..4355)
                     /locus_tag="WGO87_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391741.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIKIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(4491..4934)
                     /locus_tag="WGO87_000323"
     CDS             complement(4491..4934)
                     /locus_tag="WGO87_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391742.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(4977..5846)
                     /locus_tag="WGO87_000324"
     CDS             complement(4977..5846)
                     /locus_tag="WGO87_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0391743.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            6010..6933
                     /locus_tag="WGO87_000325"
     CDS             6010..6933
                     /locus_tag="WGO87_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0391744.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     SNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(7011..7652)
                     /locus_tag="WGO87_000326"
     CDS             complement(7011..7652)
                     /locus_tag="WGO87_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0391745.1"
                     /translation="MQKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCIAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(7707..8498)
                     /locus_tag="WGO87_000327"
     CDS             complement(7707..8498)
                     /locus_tag="WGO87_000327"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0391746.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLNVFEGRVLELPAKSHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLADLKASNPNNGKHFHL"
     gene            8664..8739
                     /locus_tag="WGO87_000328"
     tRNA            8664..8739
                     /locus_tag="WGO87_000328"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8697..8699,aa:Thr,seq:tgt)
     gene            8774..8849
                     /locus_tag="WGO87_000329"
     tRNA            8774..8849
                     /locus_tag="WGO87_000329"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8807..8809,aa:Thr,seq:tgt)
     gene            8991..9782
                     /gene="panB"
                     /locus_tag="WGO87_000330"
     CDS             8991..9782
                     /gene="panB"
                     /locus_tag="WGO87_000330"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0391747.1"
                     /translation="MITVNTLQKMKAVGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            9905..10741
                     /locus_tag="WGO87_000331"
     CDS             9905..10741
                     /locus_tag="WGO87_000331"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0391748.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            10906..12744
                     /locus_tag="WGO87_000332"
     CDS             10906..12744
                     /locus_tag="WGO87_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391749.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVVKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALTAAMMYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            12884..13465
                     /locus_tag="WGO87_000333"
     CDS             12884..13465
                     /locus_tag="WGO87_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0391750.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            13475..14320
                     /gene="ispE"
                     /locus_tag="WGO87_000334"
     CDS             13475..14320
                     /gene="ispE"
                     /locus_tag="WGO87_000334"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0391751.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            14328..14403
                     /locus_tag="WGO87_000335"
     tRNA            14328..14403
                     /locus_tag="WGO87_000335"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14361..14363,aa:Gln,seq:ttg)
     gene            14416..14491
                     /locus_tag="WGO87_000336"
     tRNA            14416..14491
                     /locus_tag="WGO87_000336"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14449..14451,aa:Gln,seq:ttg)
     gene            14500..14575
                     /locus_tag="WGO87_000337"
     tRNA            14500..14575
                     /locus_tag="WGO87_000337"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14533..14535,aa:Gln,seq:ttg)
     gene            14742..15725
                     /locus_tag="WGO87_000338"
     CDS             14742..15725
                     /locus_tag="WGO87_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0391752.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            15792..16364
                     /locus_tag="WGO87_000339"
     CDS             15792..16364
                     /locus_tag="WGO87_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0391753.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(16489..17658)
                     /locus_tag="WGO87_000340"
     CDS             complement(16489..17658)
                     /locus_tag="WGO87_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0391754.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTLQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTITEKEIVALENVEKRSRWQRLWMRLTGQ"
     gene            17806..19332
                     /gene="ilvA"
                     /locus_tag="WGO87_000341"
     CDS             17806..19332
                     /gene="ilvA"
                     /locus_tag="WGO87_000341"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0391755.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(19389..20462)
                     /locus_tag="WGO87_000342"
     CDS             complement(19389..20462)
                     /locus_tag="WGO87_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0391756.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(20459..21319)
                     /gene="cysW"
                     /locus_tag="WGO87_000343"
     CDS             complement(20459..21319)
                     /gene="cysW"
                     /locus_tag="WGO87_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0391757.1"
                     /translation="MKPYSANPNLTEPRWLRVLLTATALGFLLLMLFVPLAAVFYEAL
                     KGGWDLYLKSLSDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGRLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNTV"
     gene            complement(21574..22410)
                     /gene="cysT"
                     /locus_tag="WGO87_000344"
     CDS             complement(21574..22410)
                     /gene="cysT"
                     /locus_tag="WGO87_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0391758.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPLKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT         5657 a         5177 c         5840 g         5873 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcaccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccccctcag tctccttcat ctccttctga gaaagctccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccatcgcc ggagcggcaa tcattgtcag aatcatcatc gctgcggtaa
     1321 tttgtttttt cataataact cctttggatt acaaggttgg aaaatcaaag ccctgcttag
     1381 aacgtatgtt gcacacccaa tttcaatagg taaatcagat tgcaaatcca gcaatttgaa
     1441 tattgtcatt gttccgtgca aaagggattt ttattgatga gttgtgtact gggtttcagc
     1501 ttggcttttt agataatctt ttcacaagaa catacatcaa tactgtccaa aaaaaagatt
     1561 tgataaatat attgcaacat attttattcc acctccatcc gaaacagaaa ccaatatatt
     1621 tttaattaat acatagccga ttattacatt tattattcct ataatatata aggatcttgc
     1681 caaaattttt tttgatttta ttttaggaat atctcttatc catgctaaaa tacagcccaa
     1741 tgtcgaaaag aaaataacag caaacactac cttacctcct ctcacttcca accaaaccga
     1801 tagcaggttt ggttggaagt tggtttattt attatttaac ggcgaatgtc agtgttctta
     1861 cccgtagaac ctgcataacc agttatgcca ccagcaacag tccccactgc tacacctcgc
     1921 ataccattaa tcagaattct tcctattttt tcgaatccac tatttcttcc aaagatgcag
     1981 caaacgcagg ttgagcctag acccaatcta cgtttgctgc cgcatctgat ccctattgtt
     2041 tcttatcctt acatcttcct gccttgtcaa tcaaataaag acagaagact atacaaaaac
     2101 tgaccgacat actgaaaata cctatcccct tccatgcaat ctccgcacca ttgacccaat
     2161 agatcagact gagaaacaac aaagccacag tataaatcaa cgtaaaaaat attgcgtaaa
     2221 gcacaataaa gggaactttt atctcacggt tgttttttat aatatattca gcaacttgat
     2281 ttccgaatat acctgataaa aaatatagta ttagatcatc catttcgttt atcttctatg
     2341 ttttcccatt gcggcgctag aagactgatt taatgctgca ccatttgcac gaataactgc
     2401 attaggaata gcatttcccc ctttccaagc agaacgggca aaaacactag tagtaatccc
     2461 tgcaccgcgc aacatcgcac tgctatatcc gcctcctatc ataccaccag cggttgcagc
     2521 caaggtactt cttgttgaag caaattgccc tgttttaatt ttagaaatgc cgtgattagc
     2581 ccaagcacta attgcccctc ccatcaaagc acctgccaca ataggaagaa acgccccctc
     2641 agtctccttc atctcctttt gagaaagctc cgccaactgc atcggcgcat ctgcctgcgt
     2701 gtggaatacg tatttttcaa atgcctgatt gtccaatccg tttgccattg cgggggcgat
     2761 tatagacagc atcatcgtgg cttcagtgat ttgttttttc ataacaaatt cttagggagg
     2821 agataatgtt ccatcaaaat cctcctgaaa ggctgcctga aatttccaag cagccttttt
     2881 gattgcttat taatcggata gtgcgacaaa tataaagtgg gcggcaatca ttgccgtaac
     2941 ggcagccata actgttttat tttcttcaaa ccaaagcaag ttgagaaaca taaacatcaa
     3001 gaaatatgat ggttcttcag ttttgccgct gctttcttga gttagaagat aaaatataga
     3061 tatggcggtt atgccgattg ctgcttttat taaaatatat ttgtttttca tcatcttgta
     3121 tggtttttca tgcaagtttc aacaataaaa ttcggaataa gatagagaga tgttgatatt
     3181 tcagataaat atacaaaaca atcaagaaac tattggcggt atgtataatc ttgaatttct
     3241 ttttttgatg aaattaaaat aatgaaatat atggtaaacg ttacaacgga agccttagcc
     3301 tcccgattgc agtatttcaa ttccaagata aatgccgcat agaaaagatt aaatacttaa
     3361 atggttttta gcggcttaaa tatcttattt aattttattg attgttttcc taatagtttc
     3421 tgcaataaat aataagaaat agaataataa acaaacagaa catgaaaata aaaataaatc
     3481 tttcattatc ccagtaataa aactccaaag attttcctta tttccatcaa caataaaaat
     3541 tatcagttca tgaataaaga atactgcaaa gaaaatgctt cctgccgtta gagatatttc
     3601 taatctgctt attttccctt cgctatattt acctgataaa tagcctaaaa aagctgcaat
     3661 caaatcatcc attttaaaaa tttctgtaaa aacaggtaat gtctttaatt cattacctgt
     3721 tttgccatta tttatagtgg attaaattta aatcaggaca aggcgacgaa gccgcagaca
     3781 gtacaaatag tacggcaagg cgaggcaacg caacgccgta ctaatccact atatatatcc
     3841 attcatatag agaaaaattc ttatacccaa tataaaacaa ggtctttcat tatattttga
     3901 aaaacctaga agctttgtat ctattggttt atcaaaatta ataattattg cagaagacat
     3961 gtaggctaat aatcttgatt tatctatatt ttattaatta tatatttaac cccgaagtca
     4021 atatagaaat ttagtgattt taaagttctc ttatcaattt taatctcagg gagtacataa
     4081 aaatcctcag aataggcaaa ctctatatat gcatcatcat tcgataataa ttcaataata
     4141 tttttcattt catccgaaag aaatgtaacc atctcatatt gacatttatt tttagatacc
     4201 atttttgaat attgattaaa taattttgga ttactatttt tagtaattaa tccttccgga
     4261 gtaataaaac cactttgtat aaagtcatca cttatattaa cagccttccg aaaaaaaata
     4321 taatcacact ctttgttatc aatataaaat ctcatactct ttaataatga aatataagaa
     4381 tttccagaat tacaagcaat ttgaaaaaac atcattactc ctaaaactca taaatagtaa
     4441 ggaatatata tatatatgca tattccttac tattttttca caaattatgc ttatctatgc
     4501 ttatttccat aaaaagcatg ccctcgaatt ttttgacttg agttctggaa atgagaataa
     4561 tttgaatgga tttccttctg tccatgtcta ataacatttt gtcttcccca agcttggttt
     4621 gctaccgctc ttgcttgttg agatgttcgt gcatagacat tagctcctct tgataaagct
     4681 tgtgctgcaa cacgctgcgt aacccaacgc tgcgcaagga atctgcccgc atgcaaaatt
     4741 cctattgcca ccaacggtgc cgcagcccct tcagtttctt tcatctcctt ttgagaaagc
     4801 tccgccaact gcatcggcgc atctgcctgc gtgtggaaca cttggttttc aaatgtctga
     4861 ttgtccaagc cgtttgccat tgcgggggca atcatagaca gcatcattac ggctgaggtg
     4921 atttgttttt tcataataac tcctttggat tacaaggttg gaaaatcaaa acctgcttaa
     4981 aatgtatgct gtacgccaaa tttcagttcg gaactgcttt gccctgaaac gttgaaacgt
     5041 gcggatgcgt ttaaagccgt ggttttggtg aaaccgaaac ctgtgccgaa atgagcgtag
     5101 gtggatgtgt ttcttgcggt ttcttttttg ccgtctatgc ggtcgggctg tgtgcccagc
     5161 cattggatgc ctccggtcag gctgattctg tcgttggcgg caaatgagat gttggggttg
     5221 agcagcaggt agttgcccga tttgtagcgg atgccgtctg aaagggtttt gctgccgttg
     5281 atgcggtagg cggcggtgag ggaaaggaca atcggatcta tggctttgta ggtggtggcg
     5341 ccgatgagcc aggattttcc cgacgaggct ttgttgcgcg atttttcgta aaccgtgctt
     5401 tcaagaaagc tgattagggc ggggtttttg tcgtctttaa ggaaagtgtg gctgatgccg
     5461 agggatacgt cggacatccg tttgttgcgg gttttgccgt tgccgtcgag tttgcgttct
     5521 tcgtgccaca gatagctgcc gctgccgtaa atgtcggtat tcccggtcag tccgtagcgc
     5581 aaaccgagcg tgccgacgag catatcggta ttgctgccgt tttcttggat ttcggtcgga
     5641 atggggataa acgaggttgc gccggtttga atgtaaaccg gtgcggcaag ttcggcgcgg
     5701 ttgttttcgc tgttcaggta ggtaagggaa gtttccagtt tccattttcc cttgtcggtc
     5761 attatgtctt caatcgtcaa gggcaggtcg gcataagtgg ataaaggcag gatggcgggc
     5821 aaggcgggca aaaagatgcg cttcatattt ctcctattga tattaattct tatttactga
     5881 taataatgaa tatccgataa tcttgtctaa ataaattttt aaatcataaa aaacaatatg
     5941 tttgtttttg tcaattatgt ttcgatatgc cgtctgaaaa gtttgtgaaa aacggttaca
     6001 atccgccgca tgaaaaaacg caataatcct cttccgctgt tgaacggtgt caaacccagt
     6061 tatttggtgc tgccgcatga aaagcagttt tacgggctgc cgctgctgca ttttctgtgc
     6121 atccgctttc cttttgtggg cgcggacgat tggcgcaggc ggttgaacag cggttttgtg
     6181 gtcggttcgg atggtgcggc gttggacgaa catactttgt tcgagccggg taaggtgatg
     6241 ttttattatc gggaaaccag tcgcgagagc gagccgcgta ttccgtttga agaaaagatt
     6301 ttgcatattg atgaacattt gattgtggtg gacaagccgc attttctgcc cgtcatcccc
     6361 agcgggcgtt ttttgaggga aacgctgctc acacgtctgc gcctgcgtcc tgaattgcag
     6421 catttgaatg ttgaagacat tacgccgctg caccgcttgg acaaggatac ggcaggcgtg
     6481 atgctgctgt cgcacaatcc tgccacgcgg ggggcttatc agacgatgtt tcaaaacaaa
     6541 acggtatgga aaacgtatga ggcgcttgcc ccgacaagga cggatttgcc gtatccgctt
     6601 gatgtggttt cgcgtttggt aaggggtgaa aaatttttta cgacgcaaga ggcggaaggc
     6661 gaatcaaatg cacatacgac ggttgaacta attgaaaaca ggggggaatt cagcctttac
     6721 cgccttacgc cgcataccgg caagaaacac cagttgcgcg tacacatgat gggcttgggt
     6781 atgccgttat tgaatgacgc gctctatccc gtgccgtctg aagcgggcag cgaggattat
     6841 cggaaacctt tgaagctatt ggcaaaaaag attgcgtttg cagatccttt gtcgggtcgg
     6901 gaaagggtgt tttgcagcgg attttgccta taatgagagg gaacgcaaac ccgtatcggc
     6961 ggatgccgcc gatacgggtt ttacattatc gatgccgtct gaaaggggtt ttatccgttc
     7021 aggcatacgg gcgcgaagcg gaggatggtg tcgcgcagga cgatgagttt gccgtgaaag
     7081 aaatgcgtgg aaccggcgat ggtaatgacg ggcaaatctt gcggttccgc ccatttcagt
     7141 gctttcccga tttcgacgac ttcgtcttcc gcgccgtgta tcatcagcgt tttggcaaca
     7201 ttgggaacgg cggacggctc ggggcggccg gtatagtggc agactgccgc gccgatgagc
     7261 agcaataaat cgggtatgcg cgcttgtgcg gcaaatgtgg cgacataacc gccgaaggag
     7321 aagccggata aggcaaattc gggggcttcg ggatgttggg cgcgggcata gtcgatgacg
     7381 gcgatacagt cttgcgtttc accgcgtccg taatcgtgtg tgccttcgct gccgcctacg
     7441 ccgcgaaggt tgggcaggta gcagtggaag ccgagtttgc ttaaggcttt ggcggcggtt
     7501 tggatgactt tgttggtgtt cgtcccgcct tggagggggt tggggtggtt gatgacggca
     7561 acaccgcgtg ccggtacttg ttcggacggg atatggatgg tttctaaaat gccggcaggg
     7621 ccgggtatgt gtatggtttc aggtttttgc ataatggttt cctcttgatt ttccgcatat
     7681 tccgatgcag ccgcgaagat ggcggcttaa aggtggaaat gcttgccgtt gttggggttg
     7741 gatgctttca ggtcggcgag catgcggtgg aagtccaatc cgtaagcgtt gctcagtttg
     7801 tcggcacgtt ttttgagttt gtctatgttt tgctctttgg ggctgctttg gaatgccatt
     7861 accgcgacat tgccgtgact tttggcagga agttccaata cgcgcccttc aaagacgttt
     7921 aacaggcgtt cgatgaagcg ttgatagcgt ttgtcgccgc tccaccagtt ggtaacgaat
     7981 atgccgtctg aagagagtgc gttgcggcag tctcggaaga acggttcttc aaccagcgta
     8041 tcgatgattt gttcgccgtc gaatccgtcc accaagatga tatcggtgtt gtggcggaag
     8101 actttgatgt actctgcacc atctgcttca ataatttcaa atttttcgcc ctcgaaaggc
     8161 aactcgaaca ggttgcgggc aatggcgata acctgcgggt tgatatctac ggcagtttga
     8221 cgcgtatcgg gcaggtaggt atctatccag cgtgcaaacg agccgccgcc caagccgatt
     8281 tgggtgatat gttgcggaag agcgtcggta aacagcagcc agcccatcat cgcgcggctg
     8341 taagagagca ccagctcgga cgggtggtcg aggttcatcg agctttgaac ggtgtcgctg
     8401 cccaagtgaa gcgaacggat attgccttct tcggaaatgc cgacttcggg aaagccggat
     8461 tttgcaggac gcaggcggcg gtagggatgt cttgccatcg gtgtgaacgg tcggtttgaa
     8521 aggcggatat tttatcagaa tgccgatgct tgttctgttt caaattaatt tcttttcaaa
     8581 taaattactt attcggattt gccggggctt tcggataaat tccttgccaa ggtgcggcat
     8641 tgcctgcata attcgctttc tttgccggta tagctcagtt ggtagagcac ctgacttgta
     8701 atcagggggt cccgagttcg actcttggtg ccggcaccat ttctgctgat ggtgttgcaa
     8761 aacatttcag taagccggta tagctcagtt ggtagagcac ctgacttgta atcagggggt
     8821 cccgagttcg actcttggtg ccggcaccag atttgacagc ccgattgtgt aaaacagtcg
     8881 ggctgttttg catttgcggc acggtcggtg gcagggctgc gggcatttca ctataatagc
     8941 ccgtttcaaa ctgaaaaggc cgtctgaaaa gggcggggta acaatatctg atgattactg
     9001 tgaacacact gcaaaaaatg aaggcggtag gtgagaaaat cgcgatgctg accgcttacg
     9061 aatccagttt tgccgcgctg atggacgatg ccggcgtgga aatgctgctg gtcggcgact
     9121 ctttgggtat ggcggttcaa gggcggcagt cgacgctgcc cgtcagcctg cgcgatatgt
     9181 gctatcacac cgaatgtgta gcacgcggtg caaaaaatgc gatgattgtc agtgatttgc
     9241 cgtttggtgc atatcagcag agtaaggagc aggcgtttgc cgccgccgcc gaactgatgg
     9301 cggcaggagc gcatatggtt aaacttgaag gcggtgtgtg gatggcggaa acgactgaat
     9361 ttttgcaaat gcgcggtatt ccggtttgtg cgcacatcgg tctgaccccg caatccgtgt
     9421 ttgccttcgg cggatataaa gttcaggggc gcggcggcaa ggcgcaggcg ttgcttaacg
     9481 atgccaaggc gcatgacgat gcgggggcgg cggtcgtgct gatggagtgc gtaccggcgg
     9541 aactggcaaa aaaggtaact gaaactgttt cctgtccgac catcggcatc ggcgcgggtg
     9601 cggattgcga cgggcaggtt ttggtgatgc acgatatgct cggcattttc ccgggtaaga
     9661 cggcgaaatt cgtcaaaaac tttatgcagg ggcatgatag tgttcaagcg gcggttcggg
     9721 cgtatgttgc cgaagtcaaa gccaaaactt tccctgctgc ggaacatatt tttgcagatt
     9781 gaacaaggtt tgctgccaat gccgtctgaa agccgtttca gacggcattt tgttttgccg
     9841 tgcgcggcgc gtataatcgg cgcgttttgt cgggcaggaa gcccgaaaga taaggattac
     9901 cgttatgcaa atcatacata ccattcgaga actgcgcgcg tggcgtaaaa atgcgggaaa
     9961 ggtggcattt gtgccgacca tgggcaatct gcatgaaggg catcttgcgc ttgtgcgtga
    10021 ggcgaaaaaa cgcgcggaca gtgtcgtggt cagcattttc gtcaaccgcc tgcaattcgg
    10081 tcagggcgag gatttcgaca aatatccgcg tactttgcaa caggatgcgg acaagctgga
    10141 agccgagggt gttgccgttg ttttcgcgcc cgatgagaaa gaactctatc cgaacgtgga
    10201 acagcgttac aacgtcgaac cgcccaatct gcaaaatgag ttgtgcggca agtttcgtcc
    10261 gggacatttc cgcggggtgg cgacggtggt gtccaagctg ttcaatattg tcgcgccgga
    10321 tgttgcctgt ttcggcaaaa aagactatca gcagcttgcc gtgattaaag gtttgaccga
    10381 ggatttgaat tttgatgttg aaatagtgcc tgtcgatacg gggcgcgcgg aagacggttt
    10441 ggcactctcc agccgcaacc agtatttgag tgcggcggaa cgcgacgaag caccgcgcct
    10501 gtaccgcgaa ttaaaggctg ttgccgaatc cttggcgcag ggcagtttgg attatgcagg
    10561 tttggaaaaa cgtgccgtcc aatccctgac agaatacggc tgggtggtcg attatgtcga
    10621 aatccgccgc gccgatacgc tcgaagtggc gcgggcggga gataagaaac tggtggtctt
    10681 ggccgccgcc cgtctgggga cgacgcgcct gattgacaat ttggaaataa aactccctta
    10741 aactgcaagc gtcgggaatg ccgtctgaag cggatttgcg tttcagacag catttatttt
    10801 tttgaacggg gtttcgcaaa tctacaaacg attctgcttg tgataaagtt acgcctgatt
    10861 atatgccgtc tgaaggttcg gacggctgtc ggataaagga agattatgtt acccgcccgt
    10921 ttcaccattt tatctgtgct cgcggcagcc ctgcttgccg ggcaggcgta tgccgccggc
    10981 gcggcggatg cgaagccgcc gaaggaagtc gtaaaggttt tcagaaagca gcagcgttac
    11041 agcgaggaag aaatcaaaaa cgaacgcgca cggcttgcgg cagtgggcga gcgggttaat
    11101 cagatattta cgttgctggg aggggaaacc gccttgcaaa aggggcaggc gggaacggct
    11161 ctggcaacct atatgctgat gttggaacgc acaaaatccc ccgaagtcgc cgaacgcgcc
    11221 ttggaaatgg ccgtgtcgct gaacgcgttt gaacaggcgg aaatgattta tcagaaatgg
    11281 cggcagattg agcctatacc gggtaaggcg caaaaacggg cggggtggct gcggaacgtg
    11341 ctgagggaaa gaggaaatca gcatctggac ggactggaag aagtgctggc tcaggcggac
    11401 gaaggacaga accgcagggt gtttttattg ttggcacaag ccgccgtgca acaggacggg
    11461 ttggcgcaaa aagcatcgaa agcggttcgc cgtgcggcgt tgaaatatga acatctgccc
    11521 gaagcggcgg ttgccgatgt ggtgttcagc gtacaggggc gtgaaaagga aaaggcaatc
    11581 ggagctttgc agcgtttggc gaagctcgat acggaaatat tgccccccac tttaatgacg
    11641 ttgcgtctga ctgcacgcaa atatcccgaa atactcgacg gctttttcga gcagacagac
    11701 acccaaaacc tttcggccgt ctggcaggaa atggaaatta tgaatctggt ttccctgcac
    11761 aggctggatg atgcctatgc gcgtttgaac gtgctgttgg aacgcaatcc gaatgcagac
    11821 ctgtatattc aggcagcgat attggcggca aaccgaaaag aaggtgcttc cgttatcgac
    11881 ggctacgccg aaaaggcata cggcaggggg acggaggaac agcggagcag ggcggcgcta
    11941 acggcggcga tgatgtatgc cgaccgaagg gattacacca aagtcaggca gtggttgaaa
    12001 aaagtgtccg cgccggaata cctgttcgac aaaggtgtgc tggcggctgc ggcggctgtc
    12061 gagttggacg gcggcagggc ggctttgcgg cagatcggca gggtgcggaa acttcccgaa
    12121 cagcaggggc ggtattttac ggcagacaat ttgtccaaaa tacagatgct cgccctgtcg
    12181 aagctgcccg ataaacggga ggctttgagg gggttggaca agattatcga aaaaccgcct
    12241 gccggcagta atacagagtt acaggcagag gcattggtac agcggtcagt tgtttacgat
    12301 cggcttggca agcggaaaaa aatgatttca gatcttgaaa gggcgttcag gcttgcaccc
    12361 gataacgctc agattatgaa taatctgggc tacagcctgc tgaccgattc caaacgtttg
    12421 gacgaaggct tcgccctgct tcagacggca taccaaatca acccggacga taccgctgtc
    12481 aacgacagca taggctgggc gtattacctg aaaggcgacg cggaaagcgc gctgccgtat
    12541 ctgcggtatt cgtttgaaaa cgaccccgag cccgaagttg ccgcccattt gggcgaagtg
    12601 ttgtgggcat tgggcgaacg cgatcaggcg gttgacgtat ggacgcaggc ggcacacctt
    12661 acgggagaca agaaaatatg gcgggaaacg ctcaaacgtc acggcatcgc attgccccaa
    12721 ccttcccgca aaccccggaa ataatgcagg tccatccttt cagacggcat aaggtttgcc
    12781 gggaagctgc ggcattcggg caaaccgcac gcagttcgca cgcgttttgc acggcacgcc
    12841 gaacccatcg gccggcagga tggcatccgt taaggaaatt ctgatgaaac acactgtatc
    12901 cgcatcggtc atcctgcttt tgaccgcttg cgcgcaatta cctcaaaata acgaaaacct
    12961 gtggcagccg tccgaacaca cccgcagttt cacggcggaa gggcggttgg cagtgaaagc
    13021 ggaagggaaa ggttcgtatg caaatttcga ttggacatac caaccgcccg tggaaaccat
    13081 caatatcaat acccctttgg gcagtacgct cgggcagttg tgtcaagaca gggacggcgc
    13141 attggcagtg gacggcaaag gaaatgtcta tcaggcggaa agtgcggaag aattaagcag
    13201 gcagctggtc ggtttcaaac tgccaatcca atatctgcat atctgggcag atggcaggcg
    13261 tgtggcgggc gcgccttacc gcatcctgcc ggacggcata ttggaacaat acggttggac
    13321 tgtcggcaga accgccgaca gtggggggca agtccgaacg ttgcaactga ataacggaaa
    13381 tttgaacatc aggctggttt tcaccgaaat cggtatgccg tctgaaaccg aaaccccgga
    13441 acgctgtgcg gcgcgcacga gataaggcgg acagatgaat attgcggacg gacggcaggc
    13501 gtttcccgca cctgcaaaac tgaatctcga tttgaggatt actggaaagc gggaagacgg
    13561 ttatcacaat atcgaaagca tattctgcct gatagatttg caggataccg tatatttgaa
    13621 actaagggac gacggcaaaa tcatcctgca caatccggtt gatggaatgc cgcaggaagt
    13681 agatttaagc taccgtgccg catcgttgct gcaaaaatat gcgcgcaacc ccgccggcgt
    13741 ggaaatatgg ctggacaaaa aaatcccgac aggggcgggt ttgggcggcg gaagctcgga
    13801 tgcggcaacc gttttgctgg tgttgaaccg ttggtggcag tgcggtctga cgcagcggca
    13861 gctcattgat tcgggcgcgg ctctgggggc ggacgtaccg ttttttattt tcggaaaaaa
    13921 tgcgtttgcg cggggtatag gcgacaggct ggacgaaatg gatattccga aacagtggta
    13981 tgtcatcgtc aaaccgcccg tccacgtttc cactgcaaaa attttcacac acgaaagctt
    14041 gacacgggat tctgcctcaa gcataatgcc gactttccaa aacctgcaac cgtttagaaa
    14101 tgatatgcag gcagttgtat ttaaagaata ccctgaagtt tggaaagcct attccgagtt
    14161 gtcccgatat ggatttgcct taatgacagg ttctggtgcg tgtgtattca cggcgtgtca
    14221 agataggaat agcgcataca atatataccg acaagtttca gatttgtacg aggcatattt
    14281 ggcagagggt ctttcaaaac atccttggtt gtccgtataa acattgttgg ggagtcgtca
    14341 agcggttaag acactggatt ttgattccag catgcgaagg ttcgaatcct tcctccccag
    14401 ccaagtcaaa cgagttgggg agtcgtcaag cggttaagac actggatttt gattccagca
    14461 tgcgaaggtt cgaatccttc ctccccagcc agcccttttt ggggagtcgt caagcggtta
    14521 agacactgga ttttgattcc agcatgcgaa ggttcgaatc cttcctcccc agccagataa
    14581 aagcgtgtaa gcctgcttac acgcttttat ttcatagaaa taaaaatatt gaaatgcctt
    14641 tgtttgtgtc ggatgttgca ggtataatgt cgggcttggt acaagcagag ggaagcattg
    14701 tgttttctga gcggaagtta aacataaaat caggtgagaa tatggctgcg tacgacagtt
    14761 tgatggtatt tacaggcaat gccaatcccg aattggcaca acgtgttgtc aggcatttgg
    14821 acatttcttt gggcaatgct tccgtatcca agttttcaga cggcgaagtt gccgtcgaac
    14881 tgttggaaaa cgtacgcggg cgcgatgttt tcatccttca gccgacctgt gcgccgacca
    14941 atgacaacct gatggaaatc ctgacgatgg cggatgcact gaagcgtgct tcggcaggtc
    15001 gtattaccac agccattccg tatttcggct atgcgcgcca agaccgccgt ccgcgttccg
    15061 tccgcgttcc gatttctgcc aaactggtgg caaatatgct gtattcggca gggatcgacc
    15121 gtgttttgac tgtcgatttg catgccgacc agattcaagg tttcttcgat attccagtgg
    15181 acaatattta tgccaccccg attctgttga acgacatcaa acaacagcgg attgaaaatc
    15241 tgaccgtcgt cagcccggac atcggcggtg tcgtccgcgc ccgcgccgtg gcaaaatcct
    15301 tgaatgccga cttggcaatc atcgataaac gccgcccgaa agccaatgtg gcagaagtca
    15361 tgaacatcat cggcgatatt caaggcagaa cctgtctgat tgtggacgat atgattgaca
    15421 ctgcaaatac gctgtgcaaa gccgccgtcg ctctgaaaga gcggggggcg gaacgtgtct
    15481 tggcatatgc cagccacgcc gtattctccg gagaggcggt cagccgtatc gcctcatccg
    15541 aaatcgacca ggtggtcgta accgatacca ttcctttgtc tgaagcggct aaaaactgcg
    15601 accgtatccg tcaggtaacg attgccggtc tgttggccga aaccgtccgc cgcatcagca
    15661 atgaagaatc cgtttcatat ctctttaatg aagaagtgat gacaggcagt atgttgctgc
    15721 cataagcccg aagccgtctt aagctggtcg cggccgatga cggcgatttt acctaacttg
    15781 gagtatttaa catgacttat gaaattcaag cctctgttcg tgaagcacaa ggcactggtg
    15841 cgagccgccg cctgcgtcgc gaaggccaaa tccccggcat tctgtacggt gaaggtcaag
    15901 agcctgttgc aatcgctgtg gatcacaaaa ccgtattcta cgcattggaa aaagaatctt
    15961 tccataccgc gttgattaaa ttgtctctga acggcgaaac caaagacgtt atcgtccgtg
    16021 atttccaaat gcacccgttc cgccgcgaag ttcaacacat cgacttccaa gctgtgaaag
    16081 ccgatcaact tgtacgcatc cgtgttcccc tgcacatcgt taacgctgaa aattcccaag
    16141 cggtcaaact gcaaggcggc cgtgtatctc tgttgaacac gactgttgaa gtgcttgcat
    16201 tgcctgccaa tatccctgct ttcttggatt tggattgtgc cgaagtggtt gccggcgaca
    16261 ttctgcatct gtccgacatc aaactgcctg aaggtgtaga aagcgtttcc ttgaaacgta
    16321 acgaaaatct ggctgttgcc accgttaccg gtaaaaaacg ctaattgatt tcagcagcag
    16381 ggcggcgcgt atgcaatacg taccgccctg ttgttttatg ccgtctgaac cgtgtttcag
    16441 acggcatttc tttatttgtt ggaaaaacgg gatatttgaa acggcagatt actgtcccgt
    16501 cagacgcatc caaagccttt gccaccggct tcttttttct acattttcca gtgcgacaat
    16561 ttctttttcg gtaatggtgt atccgttttg cctgattttg atttttccta aaatctgtcc
    16621 cttttttacc ggggcgggaa tcggctgtat ggtttctaga atttgttctg ccattttcgc
    16681 ttccttatgt ggcagagtga tgtaggcttc tttgaggaag cctgcgcgga cggttttttt
    16741 gctgcctccg gaaatttgga tttgggcaac ggttttgcct ttcggatata ttttgggcgt
    16801 atcgaaggcc tgcaatgccc agttcagcag cttgctgttg tctgatgcgc gtgtttccgc
    16861 cgattccgaa cccaatgtga tgacaaggat gtgcctgccg ttgccggagt atgacacggc
    16921 aaggttgtag ccgccgcttt ctgtgtgtcc ggctttcaga ccgtttacat tgttgtccct
    16981 atataaaagg atattgcggt tgttttgctc tatatttttg aatttgaaag atttgatgga
    17041 aaacagcggg taatattccg gaaagtcgcg catcaatgct tcagacagct gggcgaggtc
    17101 tttggcggtg gaaacctgtc cttctctact caagcctgtc gggtttttga atacagtgtt
    17161 cttcatgccc aagcgtcggg cttctttgtt catttgttgc acaaaatttt caatcgagcc
    17221 gttgcccagc cggccggcaa gggttagggc ggcatcgttt gcggatagtg caatcatgcc
    17281 ttttaagagt ttgtcggtgc tgaccgtatc gccgggacgt acaaacattc tgcttccttc
    17341 tgaagcccat gcggattcgg gtatttttaa gttttcttca gatcggatat ttcccgattt
    17401 catgtttttg aaaaccagat atgcggtcat cagttgggtt agtgccgccg gttcgacagg
    17461 ggtattgatg tttttggcgg ataaaatctg tttgctttga aggtcgataa cgatgtgtgc
    17521 cgctgtgagg gtttcgggtg tttggagcgt gggggcggcg tgtaccgccg gtttgttggg
    17581 cgcgggcgat gcggccgttg cgtgagaaac gcctaagatg atggaaagca ggacgggcag
    17641 gattttatgt gctgtcatga aatattctaa ttgtgtgcgt gtttcagtct gccgattata
    17701 cgcttagggt gtctgatcgg gcggattttt cttgctttcg cgccgtcttg ggcgtatggt
    17761 tttgggtttt gcgattttaa taaaccgatt atcccatatt gaattatgaa cacgcccctt
    17821 ccttattcca attacctcat ccgcatcctg acggcatctg tctatgatgt cgcggtcgaa
    17881 acgcctttgg aaccggcacg cagcctttct gtacgtttga aaaacaacat ccttttgaaa
    17941 cgcgaagatt tgcagccggt tttttcgttc aaaatacgcg gcgcgtacaa caaaatgtcc
    18001 aagttgccga aagatgcgct cgcttgcggc gtgattgcgg caagcgcggg caatcacgca
    18061 caaggcgtgg cattgtccgc acagcgtttg ggttgccgtg ccgttatcgt catgccggag
    18121 actacgccga aaatcaaagt ggatgcggtc aaaagccgtg gcggcgaggt ggttttgcgg
    18181 ggcgtttcat acaacgatgc ttacgattat gcgatggagc tggcggaaca ggaagggctg
    18241 acctatatcg caccgtttga cgatcctgat gttattgcag gacaagggac ggtaggcatg
    18301 gaaattgtca gccagcatcc cgatccaatc cgcgccgtat tcgtaccgat aggggggggc
    18361 ggtttggcgg cgggcgtggc ggcatttatc aagcaggtcc gtcccgaaat caaagttatc
    18421 ggcgttcaga ccaacgattc ctgctgtatg aagcaatcgg tcgaagcggg cgaaatcgtc
    18481 catttgaaag atgtcgggct gttttcagac ggcacggcgg tcaaagtcgt cggaaacgaa
    18541 accttccgcc tctgcaaaga acttttggat gaaatcatta cagtcgatac cgatgcggtt
    18601 tgcggcgcgg tcaaggatat tttcgatgac acgcgcagca ttaccgagcc ggcgggcgcg
    18661 ttggcgttgg cgggtctgaa agcctatatc gcccgagaag gcgcggaaaa ccaaaccctg
    18721 attgccgtta ccagcggtgc gaatatgaat tttcaccgtt tgcgccatgt gtcggagcgt
    18781 agtgaattgg gcgagggcaa cgaaggtatt tttgccgtta ccatccccga agaacgcggc
    18841 agcttcctta agtttgtcaa tatattggga aataggaata ttaccgagtt caactaccgc
    18901 tacggagacg atgaaaaagc gcatatcttt gtcggacttc aagcggcagg cccgcaggat
    18961 ttggcggtta tcggcagccg gttggatgag gcgggattgc ccaatgtcga cctgactgat
    19021 gatgagattg ccaaaatcca tatccgttat atggttggag ggcggacgga caaagtagaa
    19081 aacgagcgtt tggtcagttt tgagtttccg gagcgtccgg gggcgttggc gcgttttttg
    19141 aaccatatgc agggcggttg gaacattacg ctcttccatt accgcaacca cggtgcggat
    19201 tacgggcgga ttttggtcgg tatcgatgtg ccgccgcacg atgccgccgc atttgacggt
    19261 ttcttggaaa gtctgggata cagctatcac gaggaaacgc aaaatgccgc gtacaagctg
    19321 tttcttgcct gacgcttgaa agcacaatgc cgtctgaaag cctttcagac ggcattgcgc
    19381 tttcatggtt aaatcgaata ttcaatcagt tcgttttgag agaagacata aacctgtttc
    19441 ggaatcagct tcaattcttt gccttcggcg attgggtaac gcgcggcatc gctgccagcc
    19501 agcgtgatat gcacgtcttg tttgccgtgt ttcaccaaaa tatgcgtcaa tgccccgacg
    19561 gcgtggactt tttcgatttc ggcacaaatc atcggtgttt cgtgttcggc ggcgatctgc
    19621 cattcgtgcg ggcggatata gccggtggcg gtttgttcct gccatttgta ttgtgcgtcc
    19681 aatttccacg cgaagccgtt ataatgccag aagccttttt cgatgcgtcc ttcaaaagcg
    19741 tcggtttcgc cgaggaactc ggtaacgaag gcattttcgg gtttgcggta aatagcttcg
    19801 gcgctgccgg tttgttcgat tttgccgtgg ttcatcacga cgatttcgtc ggaaacttcg
    19861 agggcttctt cttggtcgtg cgtaaccaga atgctggtta cacccaggtt gtgatggata
    19921 tcgcgcagcc aggtgcgtaa ttctttgcgt actttggcgt ccaacgcgcc gaagggttcg
    19981 tccaaaagca ggagtttcgg ttcgaccgca agcgcacggg cgagggcgat gcgctggcgt
    20041 tgcccgccgg agagttggtg cggataggat tttgccaaat gagagagctg cacgagcttg
    20101 agcaattctt cgactttggc gcggatttgt cctttggacg ggcgttcgga cttgggcaat
    20161 acggtcaaac cgaaagcgac gttgtcaaac acgttcatgt ggcggaagag ggcgtagtgt
    20221 tggaacacga agccgacttt gcgctcgcgc acgtgtttgg cggttacgtc ttgcccgtca
    20281 aagaggattt ggccgccgtc ggcgttttcc agtccggcga taatgcgtaa aagcgtggtt
    20341 ttgccgcagc cggacgggcc gagtagggaa acgagtttgc cggtggggac gttgaggttg
    20401 atgtttttca gcgcgtgaaa attgccgaag tgtttgttta agttttggat ggtgatactc
    20461 atactgtgtt cctttcggcg gcggcgagtt ttttgtcttg taatttggta atgatgtttt
    20521 gcaccgccag cgtcgccagt gctaaaagtg ccaatacgcc ggagagggcg aatgcgccgg
    20581 taaagttgta ttcgttgtag aagatttcga ccaaaagcgg gacggtgttg gtttcgccgc
    20641 gtatgtgtcc cgataccacg ctgaccgcgc cgaattcgcc catcgcgcgg gcgttggtga
    20701 ggatgatgcc gtagagtaac gcccatttga tgttgggcag ggtaacgcgc caaaacatct
    20761 gccagccgct tgcgccgagt atcagcgcgg cctgttcttc gctgtcgccc tgcgcctgca
    20821 ttaaaggaat gatttcacgc gcgacaaagg ggaaggtaac gaacaaggtc gccaaaataa
    20881 tgccggggat ggcaaagata atctgtatgc cttgcgcttc gagccggcca cccaatgccg
    20941 tgtgcgcgcc gaacaataag acgaacatca aaccggctac cactggcgat acggaaaacg
    21001 gcaaatcgag cagggtggtc agcaactgct tgcctcggaa gtcaaaacgg gtcagcagcc
    21061 acgccatcgc cacgcccaag acggcattga cggggacgac aatcagcgca gtaatcagcg
    21121 tcaatttgat ggcagaccac gcttcgggat cgcttaagga tttcaggtac aaatcccaac
    21181 cgccttttaa ggcctcgtaa aacacggcgg cgagcggcac gaacagcatc agcaacagaa
    21241 agcccaaggc ggtagcagtc agcaatacgc gcagccaacg cggttcggtc aggttgggat
    21301 tggcggaata gggtttcatg gcggtggtgt tccccggttg gttgttttaa ggtgttcgga
    21361 tgccgtctga aaaaccgttt tttgtggggc ggattcgttt tcagacaacc ttttattgat
    21421 taagggaata aaaacgtcta gctaccgtca ttcccgcgca ggcgggaatc caccacaggg
    21481 caacaggaaa acagaaaata aataaggcag ccgaaattca ccaatggatt cccgcctgcg
    21541 cgggaataac ggtaacaggt atttcagacg acctcaaccc ttcgcgcccg aacgcctgcc
    21601 caacgcccac tgcatcacgt tcagcgcaaa cagaatcaca aacgaaacca gcagcataaa
    21661 caacgccacc gccgacgcgc cctgtacgtc gaactgttcc agcttgcccg taataatcag
    21721 cggcaggatt tcagaaacca tcggaatatt gcccgcgata aaaatcaccg agccgtattc
    21781 ccccgttgcc cgcgcgaaca tcattcccgc gccagtcaag agtgccggcg tgatttccgg
    21841 cagcagcacg cggcggaacg tcgtaaagcg gtttgcgccc aaagtcgccg ccgcttcctc
    21901 atattcgccc gataattcct ccaacaccgg ctgcacggcg cggacgataa agggcaggct
    21961 gacgacgacc agcgcaatcc aaatgccgac gggtgtaaac gcgattttga tgcccaaagg
    22021 ctcgaaaaaa cggcctatcc aaccgttggg cgcatacagg gtcgccaacg cgatacccgt
    22081 aaccgccgtc ggcagcgcaa acggcaaatc gaccagcgcg ttcgccagac ccttgagcgg
    22141 aaattcatag cgcaccaaca cccacgccac cagcgtgccg aacacgacat tggtcagcat
    22201 cgcataaaac gacatccgca aactcagcca caccgccgcc aacacgttcg gctcggcaat
    22261 cgtgttccaa aagccgcccc agccgatttc cgccgccttc gccgccatca tcgcaaacgg
    22321 caagaccaca agcagcgaca ggcacaatac ggtcagcccg aggctgagtt tgaagccggg
    22381 cagtacgccg ggcgttttga gcggtaacat aaaacaatgc tgaaaataag gaaaaggaag
    22441 gactacttta acgatgccgt ctgaaaaacg gaaagaatgg aaagtttggt gcaaagacga
    22501 atttgttata aagcggttgg cagtttctca agcgggcgcg atgtttt
//
LOCUS       DAWFBO010000012        22486 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFBO010000012 DAWFBO010000000
VERSION     DAWFBO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.012"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..744
                     /locus_tag="WGO87_000345"
     CDS             <1..744
                     /locus_tag="WGO87_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEZ0391759.1"
                     /translation="TNIVKIDEKLEAVADTVDKHAEAFNDIADSLDETNTKADEAVKT
                     ANEAKQTAEETKQNVDAKVKAAETAAGKAEAAAGTANTAADKAEAVAAKVTDIKADIA
                     TNKDNIAKKANSADVYTREESDSKFVRIDGLNATTEKLDTRLASAEKSIADHDTRLNG
                     LDKTVSDLRKETRQGLAEQAALSGLFQPYNVGRFNVTAAVGGYKSESAVAIGTGFRFT
                     ENFAAKAGVAVGTSSGSSAAYHVGVNYEW"
     gene            complement(810..1148)
                     /locus_tag="WGO87_000346"
     CDS             complement(810..1148)
                     /locus_tag="WGO87_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0391760.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            1299..5255
                     /gene="purL"
                     /locus_tag="WGO87_000347"
     CDS             1299..5255
                     /gene="purL"
                     /locus_tag="WGO87_000347"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0391761.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKRQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGADKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHYPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQQDRDYVDAAFEAAG
                     LTGAVSRIGSPDFDNESISFFGYGYFLEQNRADLQRAWQETSHAIQKLRDNPACADSE
                     FALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKSAS
                     LILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG"
     gene            5350..6108
                     /gene="gloB"
                     /locus_tag="WGO87_000348"
     CDS             5350..6108
                     /gene="gloB"
                     /locus_tag="WGO87_000348"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0391762.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            6422..10899
                     /gene="mspA"
                     /locus_tag="WGO87_000349"
                     /gene_synonym="ausI"
                     /pseudo
     CDS             6422..10899
                     /gene="mspA"
                     /locus_tag="WGO87_000349"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
     gene            11131..12585
                     /gene="mgtE"
                     /locus_tag="WGO87_000350"
     CDS             11131..12585
                     /gene="mgtE"
                     /locus_tag="WGO87_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0391763.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(12671..13579)
                     /gene="hslO"
                     /locus_tag="WGO87_000351"
     CDS             complement(12671..13579)
                     /gene="hslO"
                     /locus_tag="WGO87_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0391764.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            13824..14579
                     /locus_tag="WGO87_000352"
     CDS             13824..14579
                     /locus_tag="WGO87_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0391765.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            14598..14816
                     /locus_tag="WGO87_000353"
     CDS             14598..14816
                     /locus_tag="WGO87_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391766.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            14961..15305
                     /locus_tag="WGO87_000354"
     CDS             14961..15305
                     /locus_tag="WGO87_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0391767.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            15305..15997
                     /locus_tag="WGO87_000355"
     CDS             15305..15997
                     /locus_tag="WGO87_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0391768.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            16064..17284
                     /gene="argJ"
                     /locus_tag="WGO87_000356"
     CDS             16064..17284
                     /gene="argJ"
                     /locus_tag="WGO87_000356"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0391769.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            17344..18780
                     /locus_tag="WGO87_000357"
     CDS             17344..18780
                     /locus_tag="WGO87_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0391770.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETTAQTG
                     NAPARPQTAKRECRLKIKTPPIKRRQPP"
     gene            18935..20326
                     /gene="hrpA"
                     /locus_tag="WGO87_000358"
     CDS             18935..20326
                     /gene="hrpA"
                     /locus_tag="WGO87_000358"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0391771.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     VAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKQRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRISAGV
                     CIRLFSEEDFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            20427..21554
                     /locus_tag="WGO87_000359"
     CDS             20427..21554
                     /locus_tag="WGO87_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391772.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANINEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            21731..>22486
                     /locus_tag="WGO87_000360"
     CDS             21731..>22486
                     /locus_tag="WGO87_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0391773.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRHKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSRIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKP"
BASE COUNT         5556 a         6929 c         5520 g         4481 t
ORIGIN      
        1 acaaatatcg taaaaattga tgaaaaatta gaagccgtgg ctgataccgt cgacaagcat
       61 gccgaagcat tcaacgatat cgccgattca ttggatgaaa ccaacactaa ggcagacgaa
      121 gccgtcaaaa ccgccaatga agccaaacag acggccgaag aaaccaaaca aaacgtcgat
      181 gccaaagtaa aagctgcaga aactgcagca ggcaaagccg aagctgccgc tggcacagct
      241 aatactgcag ccgacaaggc cgaagctgtc gctgcaaaag ttaccgacat caaagctgat
      301 atcgctacga acaaagataa tattgctaaa aaagcaaaca gtgccgacgt gtacaccaga
      361 gaagagtctg acagcaaatt tgtcagaatt gatggtctga acgctactac cgaaaaattg
      421 gacacacgct tggcttctgc tgaaaaatcc attgccgatc acgatactcg cctgaacggt
      481 ttggataaaa cagtgtcaga cctgcgcaaa gaaacccgcc aaggccttgc agaacaagcc
      541 gcgctctccg gtctgttcca accttacaac gtgggtcggt tcaatgtaac ggctgcagtc
      601 ggcggctaca aatccgaatc ggcagtcgcc atcggtaccg gcttccgctt taccgaaaac
      661 tttgccgcca aagcaggcgt ggcagtcggc acttcgtccg gttcttccgc agcctaccat
      721 gtcggcgtca attacgagtg gtaagcagca tctcccgata aagaaaccgc agccctgcaa
      781 ggctgcggtt tttattttct atccggccgt cagactgctg cgtccgcacg ttcgcccgtg
      841 cggatacgga ttgcctcctc gaccggcagc acaaaaatct tgccgtcgcc gatttttccc
      901 gaacgcgcca cctcgacaat cacgtcaatc gcgcgttcca cagcgtcatc cgccaacacc
      961 agctcgattt tgaccttggg caggaaatcg acagcgtatt ccgcgccccg gtaaatctcc
     1021 gtatgcccct tctgcctgcc gaaccctttg acctcgctga cggtcatacc cgtaatgccg
     1081 atttccgtca acgcctcgcg cacgtcgtcg agtttgaacg gtttgacaat cgcctcgatt
     1141 tttttcataa aatttccttt gaacaaacaa tacaaacaca tccgaaaaac gggaacctcc
     1201 cgtcagattg tcaacatttt aagccaaaat acccaagcaa tacagccccg ttgcgcgtat
     1261 aatgacagat tttccaaccg catttgagag ccgaatccat gtctgtcgtt ttgcccttgc
     1321 gcggcgttac cgccctttcc gatttccgtg tcgaaaaact cttgcaaaaa gccgccgcac
     1381 tcggactgcc cgaagtcaaa ttaagcagcg aattttggta tttcgccggc agcgagaaag
     1441 cattggatgc cgcgactgtc gaaaaactgc aagccttgtt ggcggcgcaa agcgttgaac
     1501 aaacgccaaa agcgcgcgag ggcttgcatt tgtttttggt aacgccccgt ttgggtacga
     1561 tttcgccgtg ggcttccaag gcgaccaata tcgcggaaaa ctgcggtttg gcaggcatcg
     1621 agcgcatcga gcgcggcatg gcggtgtggc tggaaggtgc gcttaccgat gaacagaaac
     1681 ggcaatgggc ggcgttgctg cacgaccgca tgaccgaatc ggtgctgccc gattttcaga
     1741 cggcatccaa attattccac catctcgaat ccgaaacctt ttccaccgtt gatgttttag
     1801 gcggcggtaa agaggctttg gtcaaagcca ataccgaaat gggtttggca ctctccgccg
     1861 acgaaatcga ttatctggtt gaaaactatc aggctttgca gcgcaatccg tccgatgttg
     1921 aattgatgat gttcgcgcag gcaaacagcg aacactgccg ccacaaaatc ttcaacgccg
     1981 atttcatcct caacggcgaa aaacagccga aatccctgtt cggtatgata cgcgacacgc
     2041 acaacgcgca tcccgaaggc acggtcgtcg cttataaaga caattcgtcc gtaattgaag
     2101 gcgcgaaaat cgagcgtttc tatccgaatg cggcggaaaa ccaaggttac cgtttccacg
     2161 aagaagacac gcatatcatc atgaaagtgg aaacgcacaa ccacccgacc gccatcgcgc
     2221 cgtttgcggg cgcggcaaca ggcgcgggcg gcgaaatccg cgacgaaggc gcgacgggca
     2281 aaggttcgcg tccgaaagca ggcttgaccg gctttaccgt ctccaacctc aacatccccg
     2341 acctcaaaca gccgtgggaa caagactacg gcaagccgga acacatttcc tcgccgttgg
     2401 acattatgat tgaaggcccc atcggcggcg cggcgttcaa caacgaattc ggccgcccca
     2461 acctcttggg ctacttccgc acttttgaag agaagtttga cggtcaggtt cgcggctatc
     2521 acaaaccgat tatgattgcc ggcggcttgg gcagcattca ggcgcagcag acgcataaag
     2581 acgaaatccc cgaaggcgca ttgctgattc aactgggcgg cccgggcatg cttatcggct
     2641 tgggcggcgg cgcggcttct tcgatggata ccggcacgaa cgacgcgtct ttagacttca
     2701 attccgtaca acgcggcaac cccgaaatcg aacgccgcgc gcaggaagtc atcgaccgct
     2761 gctggcagct cggcggcaaa aacccgatta tctccatcca cgacgtgggc gcgggcggtt
     2821 tgtccaacgc cttccccgaa ctcgtcaacg atgccggacg cggcgcggta ttcaagctgc
     2881 gcgaagtgcc gttggaagaa cacggtttaa cgcctttgca aatctggtgt aacgaatcgc
     2941 aagagcgtta tgtgttgtcg attttggaaa aagatttgga caccttccgc gccatctgcg
     3001 aacgcgaacg ctgcccgttt gccgtggtcg gcacggcgac cgacgacggc catttgaaag
     3061 tgcgcgacga tttgttcgcc aacaaccccg tcgatttgcc gctgaacgtc ttgctcggca
     3121 aaccgcccaa aaccacgcgt accgacaaaa cggttacgcc gtccaaaaaa ccgtttcacg
     3181 ccgacgatat cgacatcacc gaagccgcct accgcgtttt gcgcctgcct gccgtagccg
     3241 ccaaaaactt cctgattacc atcggcgacc gcagcgtcgg cggcatgacc caccgcgacc
     3301 aaatggtcgg caaataccaa acccccgtag ccgactgcgc cgttaccatg atgggcttca
     3361 atacctatcg cggcgaagca atgtctatgg gcgaaaaacc gaccgttgcc ctgtttgacg
     3421 cgcctgcttc gggcagaatg tgcgtcggcg aagccatcac caacatcgcg gcggtcaaca
     3481 tcggcgacat cggcaacatc aagctctccg ccaactggat ggcggcgtgc ggcaacgaag
     3541 gcgaagacga aaaactctac cgcaccgtcg aagcggtttc taaagcctgt caggcactgg
     3601 atttgagcat ccccgtgggc aaagacagcc tgtcgatgaa aaccgtttgg caggatggcg
     3661 cggacaagaa atccgtcgtt tcgccgttga gcctgattat ctccgcgttc gcgccggttc
     3721 aagacgtacg caaaaccgtt acgcccgaat tgaaaaacgt cgaagacagc gtattgttat
     3781 ttatcgattt gggcttcggc aaagcgcgta tgggcggctc ggcgtttggt caggtgtaca
     3841 acaatatgag cggcgacgcg cccgatttgg acgatacaag tcgtctgaaa gcgttttaca
     3901 acgtgattca acagcttgtc gccgaagaca aactcttggc ataccacgac cgcagcgacg
     3961 gcggtttgtt tgcgacgctg gcggaaatgg cgtttgcggc acgttgcggt atcagtgccg
     4021 atatagattg cctgatggat aaattcctgc cgattcatta tccggatttc caaggcgacc
     4081 cggccgaaga cttatctgac gaactttata atcatgccgc cattaaaatc ttatttaatg
     4141 aagaattagg tgcggttatc caaatccgcc aacaagatag ggattatgtt gatgcggcat
     4201 ttgaagcggc cggcttaacc ggtgcggtca gccggattgg ctctcctgat tttgacaatg
     4261 aatctatttc tttctttggt tacggttatt tcctagaaca aaaccgtgcc gacctgcaac
     4321 gcgcatggca ggaaacttct cacgccattc aaaaactgcg cgacaacccc gcctgcgccg
     4381 acagcgagtt tgccctgatt ggcgacaacg accgcagcgc attgtttgcc aacctgaaat
     4441 tcgacgtgaa cgaagacatc gccgcgccgt ttatcaacag cggcgcgaaa cccaaaatcg
     4501 ccatcctgcg cgaacagggc gtaaacgggc aaatcgaaat ggccgccgcc tttacccgcg
     4561 ccggtttcga tgcttacgac gtgcatatgt ccgacctgat ggcaggccgt ttccgccttg
     4621 ccgacttcaa aatgctggcg gcgtgcggcg gcttcagcta cggcgacgta ctcggcgcgg
     4681 gcgaaggctg ggcgaaatcc atcctgttcc accctgctct gcgcgaccag tttgccgcat
     4741 tcttcgccga cccggacacg ctgacattgg gcgtgtgcaa cggctgccaa atggtcagca
     4801 acctcgccga gattatcccg ggtgcagagg catggcctaa attcaaacgc aacctgagcg
     4861 aacagtttga agcacgcctg agcatggttc acgtccccaa atccgcgtcg ctgattctga
     4921 acgaaatgca aggctccagc ctgcctgtcg tggtcagcca cggcgaaggc cgcgccgact
     4981 tcgcgcttca cggcggcaac atttctgctg atttgggcat cgcgctgcaa tatgtggacg
     5041 gacaaaacca ggttacccaa acctatccgc tcaaccctaa cggctcgccg caaggcatcg
     5101 ccggtgtgac caacgccgac ggccgcgtta ccattatgat gccgcatccg gaacgcgtgt
     5161 accgtgccgc gcaaatgagc tggaaaccgg aaggctggac ggaactgtcc ggctggtacc
     5221 gcctctttgc cggcgcacgt aaagccttgg gctaaccgcc ctactcaaac caatgccgcc
     5281 tgaagaatat ttcagacggc gttccggcac accatccttt aaacggtatc cgtccaccga
     5341 ggaacactca tgaaaatcac acccgtcaaa gccttgaccg acaactacat ttggatgata
     5401 cagcacggca accatgccgt ctgcgtcgac ccttccgaac cctcgcccgt cttggaattc
     5461 ctcgtccgca accgcctcat gcttgcccaa acatgggtaa ctcaccccca tcccgaccac
     5521 gagggcggtg cggcggcact ctggcgcggc tacatggaat cgcccgttta cggcgaatcc
     5581 gacatcgaag cagcaaccca caccgtaacc gccggcaccc aattcacctt cggcgacgga
     5641 caggttaccg tttgggcaac acccggccac acagaccgcc acaccagcta ccttctcgaa
     5701 acttcagacg gcatacacgt cttttgcggc gacacccttt tttccgccgg ctgcggacgc
     5761 gtgtttaccg gcacaatcga acagctttac gacagcttcc aacgcttcaa ccgcctgcct
     5821 gaaaacaccc tgttctatcc ggcgcacgaa tacaccgccg ccaacctgcg tttcgccgcc
     5881 catatcgagc cggacaacgc cgacattcag acggcactga aagcggcggc gcatacgcct
     5941 accctgcccg ttaccctcgc gcacgaacgc cgcgtcaatc cgtttttgcg cgtcgacctg
     6001 ccgcacgtca gagaccgcgc cgaggcattg agcgggaaaa cgttaaacag cagcctcgat
     6061 acctttgtcg cgctgcgtga acttaaaaac caataccgga cgaaataaaa caacgggaaa
     6121 acgcagccat tcctaggatt tttattaaaa tcttaaataa aatcatacaa tcatcgccaa
     6181 tagacgaaag gacaccgttg ccttataatc aaacaaaaac aaaatatata atatagtgga
     6241 ttgaatttaa atcaggacaa ggcgacgaag ccgcagatag tacggcaagg cgaggcaacg
     6301 ctgtactggt ttttgttaat ccactatatt gttaatccac tatataaatc cagcacaaaa
     6361 cgggatcggt gattcttgtc cgcaagaatc gttgattttc tctattacac ggataatcat
     6421 catgcgcttc acacacacca ccccattttg ttccgtattg tccaccctcg gtctttttgc
     6481 cgtttcccct gcttactcat ccattgtccg caacgatgtc gattaccaat attttcgcga
     6541 ctttgccgaa aataaaggcg cgttcgccgt aggtgcaagc aatatttcca tccaagacaa
     6601 gcaaggcaaa atattaggca gggttctcaa cggcatcccc atgcccgact tccgagtcag
     6661 caaccggcaa accgccatcg ccaccctgat tcacccccaa tacgtcaaca gtgtcaaaca
     6721 caacgtcggc tacggttcca tacaattcgg caacgacacc caaaatccag aagaacaagc
     6781 ctatacctac cgcctcgtat cacgcaaccc gcacccggac tacgactacc accttccccg
     6841 cctcaacaaa ctggttaccg aaatctcacc tgccgcactc agcagcgtac ccttgcttgg
     6901 aaacggccag ccaaaggcca atgcctacct cgataccgac cgcttcccat actttgtacg
     6961 actcggctca ggcacgcaac aagtccgcaa agcagacggc acgcgtacac gaaccgcccc
     7021 ggcataccaa tacctgaccg gcggcacgcc gctgaaagta ttggggttcc aaaaccacgg
     7081 cttactcgtc ggcggcagcc tgaccgacca accccttaac acctacgcaa tcgccggaga
     7141 cagcggttcc cccctgtttg ccttcgacaa gcatgaaaac cgctgggtgc ttgcgggcgt
     7201 actcagcacc tacgccggct tcgataactt cttcaacaaa tacatcgtca cgcaacccga
     7261 attcatccgt tccaccatcc gccaatacga aacccggctg gatgtcgggc tgaccaccaa
     7321 cgaactcata tggcgcgaca acggtaatgg cgacagcacc ctgcaagggc tcaacgaacg
     7381 catcaccctg cccattgcaa acccttcgct tgccccacaa aacgacagca ggcacatgcc
     7441 gtctgaagat gccggcaaaa cgctcatcct atccagcagg ttcgacaaca aaacactgat
     7501 gctggcagac aatatcaacc aaggcgcagg cgcattgcag ttcgacagca acttcaccgt
     7561 cgtcggtaaa aaccacacat ggcaaggtgc aggcgttatc gtagccgacg gcaaacgcgt
     7621 cttctggcaa gtcagcaacc ccaaaggcga ccggctctcc aaactgggcg caggcacgct
     7681 tatcgccaac ggacaaggca tcaaccaggg cgacatcagc atcggggaag gcactgtcgt
     7741 actcgcccaa aaagctgctt cagacggcag caaacaagca ttcaaccaag ttggcatcac
     7801 cagcggcagg ggcacggccg tcctcgccga cagccagcaa atcaaacccg aaaacctcta
     7861 tttcggcttc aggggcggac ggctcgacct caacggcaac aaccttgcct ttacccatat
     7921 ccgccatgcg gacggcggcg cgcaaatcgt caatcacaac cctgaccaag ccgcgacact
     7981 gacgctgacc ggcaaccccg tcctcagtcc cgagcatgtc gagtgggtgc aatggggcaa
     8041 ccgtccgcaa ggcaacgcgg cggtttacga atacatcaac ccgcaccgca accgtcggac
     8101 cgactacttc atactcaaac ccggcggcaa cccgcgcgaa tttttcccgt taaatatgga
     8161 aaactcaaca agctggcaat ttgtcggcaa caacaggcaa caggccgccg aacaagtcgc
     8221 ccaagccgaa aatgcccgcc ccgacctgat taccttcggc ggatacttgg gtgagaacgc
     8281 gcaaacgggt aaagccgcac cgagttacag caaaaccaat atcgaatggg tgaatagtcc
     8341 aaacacccca cacaagccct cgccctatac gaatacgcca acccgcacag gggcaggcgt
     8401 accgacgatt accgcctcaa agaaggcggc aaccccggcg cctacttccc atgggatgcc
     8461 tacagcaccc aagactggga atttctcgga cataaccggc gaagcagcca tagaaaaaac
     8521 ccgccatatc gcaaatgccg ccgtatacgg ccggcccgaa taccgttaca acggcgcact
     8581 caacctgcac tatcgtccca aacgcaccga cagcacgctg ttgctcaacg gcggcatgaa
     8641 ccttaacggg gaagtcttga ttgagggcgg caatatgatt gtgtcaggca ggcccgtacc
     8701 ccatgcctac gaccaccagg ccaaacgcga acccgttctt gaaaacgaat ggaccgacgg
     8761 cagcttcaag gctgcacggt tcaccctgcg aaaccatgcc cgactgacgg cagggcgcaa
     8821 taccgcgcat ctggacggcg acataaccgc atacaatctg tccggcatcg acctcggctt
     8881 tatccaaggc aaaacaccgg aatgctaccg ctcctaccat agcggcagca cccactgcac
     8941 acccaacgcc gttttaaaag ccgaaaacta tcgtgcacta cctgtaacgc aagtacgcgg
     9001 cgacattacc cttaacgacc gttcagagct ccgcctgggc aaagcacacc tgtacggcag
     9061 catccgtgcc ggcaaagaca ccgcagtccg catggaagca gacagcaact ggacactttc
     9121 ccagtccagc cacaccggcg cactgacgct tgacggcgca caaattaccc tgaaccccga
     9181 tttcgccaat aatacacaca acaaccgctt caacacactg accgtcaacg gcacacttga
     9241 cgggttcggc acattccgat tcctgaccgg catcgtccga aaacaaaatg cccccccccc
     9301 ctcaaactgg aaggggacag ccgcggcgca ttccaaatcc acgtcaaaaa caccggacaa
     9361 gaacctcaaa caaccgaatc gcttgcactt gtgagcctca atccgaaaca cagccaccaa
     9421 gcccgattca ccctccaaaa cggctatgcc gatttgggtg cctaccgcta catcctccgc
     9481 aaaaacaaca acggatacag cctgtacaac ccgctcaaag aggccgaact tcaaattgaa
     9541 gccacgcgtg cggaacatga gcgcaaccaa caggcataca accaattaca ggcaaccgac
     9601 atcagcagac aggttcaaca tgactctgac gcgaccaggc aggcactaca ggcctggcag
     9661 aacagtcaaa ccgaacttgc ccgcatcgac agccaagtcc aatatctgtc cgcccaattg
     9721 aaacagacag acccgctgac cggcattctg acgcgtgccc aaaacctgtg tgccgcacaa
     9781 ggatacagtg ccgatatctg ccgtcaggtt gccaaagccg ccgacacgaa cgacctgaca
     9841 ctcttcgaaa ccgaactgga tacgtatata gaacgtgtag aaatggccga atccgaactt
     9901 gacaaagcac ggcaaggcgg cgatgcgcaa gccgtcgaaa cagcccggca cgcctacctg
     9961 aacgcactca accgtctgtc ccgacaaatc cacagtttga aaaccggcgt tgccggcatc
    10021 cgtatgccga acctggccga actgatcagc cggtcggcca acaccgccgt ttccgaacag
    10081 gccgcctaca ataccggccg gcaacaggcg ggacgccgca tcgaccgcca ccttaccgat
    10141 ccgcagcagc aaaacatctg gctggaaacc ggtacgcaac aaaccgacta ccatagcggc
    10201 acacaccgtc cctaccaaca aactaccaac tatgcacata tcggcatcca aaccggcatc
    10261 accgaccgtc tcagtgtcgg tacgatttta accgatgagc gcacaaacaa ccgttttgat
    10321 gaaggcgtat ccgcccgaaa ccgcagcaac ggcgcacatc tgttcgtcaa aggggaaaac
    10381 ggcgcactct ttgccgcggc agatttaggc tacagcaaca gccgtacccg atttaccgat
    10441 tatgacgggg ctgccgtccg ccgccacgca tgggatgcag gcatcaacac cggcatcaaa
    10501 atcgataccg gcatcaacct cagaccctat gccggcatcc gtataaaccg cagcaacggc
    10561 aaccggtacg tactcgacgg cgcagagata aacagcccgg cgcaaatcca aaccacatgg
    10621 catgccggca tccgtctcga taaaaccgtc gaactgggtc aagccaagct gacccccgct
    10681 ttcagcagcg attactacca tacccgccaa aacagcggtt ccgccctcag cgtcaacgac
    10741 cgtaccttac tgcagcaagc cgcccacggc acactgcata ccctgcaaat cgacgccgga
    10801 tacaaaggct ggaacgccaa acttcatgcc gcttacggca aagacagcaa caccgcccgc
    10861 cacaaacagg caggaatcaa aataggctac aactggtaac aagccgataa aaatgccgtc
    10921 tggaacccgc gtttcagacg gcatttgcgt taaaaatagt aaaccgttcc aaaagggagt
    10981 agaatagtgc cgtttccaac cctgcgcctg taccgtcagg cttttattat ggaccttccc
    11041 agttcgtttt tactgaacgc cccttccgat tccaaacgac aataaccatc ccgccggaat
    11101 gcctccccgc acacgacggg cggagcattt atgagcatcg aacacactcc tccgacacac
    11161 gacggcgaaa ccggtcaaaa ccatgccgaa cgcccttccg ctgatttcga ccgtgtccac
    11221 tccctctgcg aaatcctcga acctgccttt gaacaaatcg aaaacggcac accgctcgaa
    11281 gacgcgccgc tgcgcgacaa gctgaccgag ctgaccgtcc tcttgggcga gctgcaccct
    11341 gccgacgtgg cggcggtgtt ggaatcgctg ccgccgcgcg aacgcaacgt cgtttggctt
    11401 ctggtcaaac ccgaagacga cggcgaagta ttgctggaag tatccgacgc ggtgcgcgaa
    11461 acactgatcg agtcgatgga taaggacgaa ttgttggcgg cagttgacga tttggacgcg
    11521 gacgaattgg cggaactggc agacgatttg ccgcaccaag tggtttacga agccttacag
    11581 acgcgtgatg aggaagaacg cgcccaagtc aaggcggcaa tgtcctacga agacaaccaa
    11641 gtcggtgcga ttatggactt cgagttggtc agcatccgcg ccgatgtcgc ctgcgaagtg
    11701 gtgctgcgtt acctgcgccg cttcgacagc ctgcccgacc acacggataa aatcttcgtg
    11761 gttgacgaaa acgatgtgtt gcagggcgtg ctgcccatcc gcaaactttt ggtcgccgat
    11821 cccgaagact tggtggaaaa cgtgatggcg aaagatgtcg tgcgtttccg cgccgaagac
    11881 gacgtggaag aagcagcgca ggcgtttgaa cgctatgact tggttaccgc gcccgtcgtc
    11941 gatgaaaaca aaaagctcat cggcagggtg accgttgacg aaatggtgga cgtgatccgc
    12001 gaagaatccg aagcagatat gttgaacatg gcgggtttgc aagaagagga agacttattc
    12061 gcccccgtct tggattccgt caaaaaccgc tggatgtggc tcgccgtcaa cctctgcacc
    12121 gccttccttg ccagccgcgt catcggcgcg tttgaaggca gcatcgaaaa aatcgtcgca
    12181 ctcgccgcgc tgatgcccat cgtcgccggt atcggcggca actcgggcaa ccagacgatt
    12241 accatgattg tccgcgccat ggcgatgggg caactgacgg atatgcaggc ggggcgtttg
    12301 ctgaaaaaag aagtcggcgt cgccttggtc aacggcatca tttggggaac ggtcatgggc
    12361 gcagtatctt ggctgcttta cggcagcctc ggcatcggtt tggtcatggt tgccgcgatg
    12421 acgctcaacc tgctgctggc ggcaaccgtc ggcgtattaa ttcccgtggt aatggaaaag
    12481 ttcggacgcg atcccgcgct gggcagctcg gtgctgatta cggcggtaac ggattccggc
    12541 ggcttcctga ttttcttggg tctcgccacc ctgttcctgc tttaaatgcc gtctgaaccc
    12601 gcgcaaaaat gccgtctgaa gcggaagctg cttcagacgg catttgacta tttatccttg
    12661 ttgcacaaga ttattggacg gtatgccggg gcagcccttt ggcaacgccg accacatcct
    12721 ccccgaacag cgcgttgaca tcggtttcgt caaacacata tttgctgtgg cagaaatcgc
    12781 aatcgacttc gatgctgcct tgttccacca ccacgccgcc gacttcttcc ccgcccagca
    12841 tcagcagcat atcgctgact ttgccgcgcg aacaggtgca tgaaaattca aacgtttccg
    12901 gctcgaacac gcgcggcggc gtttcgtgga acaggcggta caaaacatgt tgcgcatcca
    12961 attctgccag ctcgtccgcc gtcagtgtac gcgccagcgt actgacgtgt tcccatgcct
    13021 cttcatccaa caccgcttca ggcagacgct gcaccagcag accgcccgcc gcttcgtcgc
    13081 ttgcagacag gacgatgtgc gtatcgattt gttcggaacg cttcatatag ttcgccaaca
    13141 tttgcgcgat accgccgcct tccaaaggca ctacgccctg ccagggttcg ccgtctttgg
    13201 gctgcagcgt cagcacgaat acgccgccct cgcccaaaag gtcgccgagg ctttcgtcat
    13261 cggctatttc tgcggtttcg tcccaacgcg cggttgcacg gacggtacgg tcggaagccg
    13321 cttccgcaac cagcattttc agccgccccc gcccctgaac ctgcacaatc agcgtgcctt
    13381 cgtttttgag gttgcccgac agcagaacac cggcagccaa cagctcgccc aaagcgcggc
    13441 ggatggcggc gggatagttt ttctgtttta caatgtgctg ccacacgttt tccagacgga
    13501 cgtgcagccc gcgcacgggc atatcgtcga agataaagcg ggtacgcaca tcggcgcggt
    13561 tgatggcggt ttgattcatg attttctctg actgattgtt cggatggcgg ctatatggtt
    13621 gcggtcggcg cgaaaacaag acggacggcg gatgcgcttc ccaaattatc aataaattat
    13681 ataaaaatca acatattaac tcaatctaac aagccgtttt ttgccaaaca gccgtttttt
    13741 tatatacaat caacaagata ttttcgactg atacagcata acatcgcacg gcggcacgat
    13801 gcctcctgcg cggaaacacc gatatggatt cttttttcaa accggcagtt tgggcggttt
    13861 tgtggctgat gtttgccgtc cgccccgccc ttgccgacga gttgaccaac ctgctcagca
    13921 gccgcgagca gattctcaga cagtttgccg aagacgaaca gcccgtttta cccatcaacc
    13981 gagcccccgc ccggcgggcg ggcaatgccg acgaactcat cggcagcgcg atggggctta
    14041 acgaacagcc cgttttaccc gtcaaccgag tccccgcccg gcgggcgggc aatgccgacg
    14101 aactcatcgg caacgcgatg gggcttaacg aacagcccgt tttacccgtc aaccgagccc
    14161 ccgcccggcg ggcgggcaat gccgacgaac tcatcggcaa cgcgatggga cttttgggta
    14221 ttgcctaccg ctacggcggc acatcgattt ctaccggttt tgactgcagc ggcttcatgc
    14281 agcacatctt caaacgcgcc atgggcatca acctgccgcg cacgtcggca gaacaggcgc
    14341 ggatgggtac gccggttgcc cgaagcgaat tgcagcccgg ggatatggtg tttttccgca
    14401 cgctcggcgg cagccgcatt tcccatgtcg gactttatat cggcaacaac cgcttcatcc
    14461 acgcgccgcg cacggggaaa aatatcgaaa tcaccagcct gagccacaaa tattggagcg
    14521 gcaaatacgc gttcgcccgc cgggtcaaga aaaacgaccc gtcccgcttt ctgaactgat
    14581 ttcccaagga atacgcaatg agtatgcccg aaatgcccaa atggtacgac gatgacggac
    14641 agatcgtgtc ctgtaccgaa aaggtcaaag tgatgtccga aaatatggcc gagctgtatc
    14701 agacggcaca agacgcgttt gaagacgcgc tgctgatggg ttgcggcgaa cgtcagttgc
    14761 gcgattacct gctcgcgctg attgaaggtt tggaaaatcc ctaccgcaaa gtctgaacac
    14821 gccccggttg ctgcggcacg gtttatccgt gccgtttttg cgtttgtgcg cggcttcggc
    14881 ttttcagacg gcatatttga cgttatgatt aaacagttaa caagatttat cacaacgccg
    14941 tcaaaagaca gacacacaac atgaacatca tccgcgcgct cctcatcatc ctcggctgcc
    15001 tcgccgccgg cgaaaccgcc gttttcctag caggcatcaa actgcccggc agcatcgtcg
    15061 gcatgggcgt gctgtttgcg cttttgcagg caggttgggt caaaacgtct tggctacaac
    15121 agcttaccga cgcgctgatg gcgaatctga cgctgtttct cgtgccgccc tgcgtggcgg
    15181 tcatcagcta tttggatttg attgccgacg attggttttc gatactggtt tccgcctccg
    15241 ccagcacttt gtgcgtactg ctggttacag gcaaggttca ccgctggata cggagcatta
    15301 tctgatgaac gaaatcctca ggcagcccag cgttctgctt ttcctcacgc ttgccgtgta
    15361 cgcgcttgcg attatcgtgc gcacgcgcac gggcaatatc ttctgcaacc ccgtactcgt
    15421 cagcactatc gtgctgattg cctacctgaa aatcctcgac atcgattatg cggtgtacca
    15481 caacgccgcg caattcattg atttttggct gaaacccgcc gtcgtcgtgc ttgccgtgcc
    15541 gctctaccaa aaccgccgta aaatcttcaa ccagtggctg cccgtcatcg tttcacagct
    15601 tgcgggcagc gttacgggca ttgttacggg gatgtatttt gccaaatggc tgggcgcgga
    15661 acgcgaagtc gtcctctcgc tcgcgtccaa atctgttacc aaccccatcg ccattgaaat
    15721 cacccgctcc atcggcggca ttcccgccat taccgccgcc accgtcatca ttgccggtct
    15781 ggtcggacag attgccggtt acaaaatgct gaagaacaca gtcgtcatgc cctcgtccgt
    15841 gggtatgtcg ctcggcacgg cttcgcacgc gatggggatt gccgcctcgc tcgaacgcag
    15901 ccgccgcatg gcggcatacg cgggactggg gctgacgttc aacggcgtac tgaccgcgct
    15961 gattgcgccg ctgctcatcc ccgttttggg attttgaacc cgtttcagac ggcatttcag
    16021 cccatgctgt ctgaacgccg acacactcgc aaggagaacc gttatggctg tcaacctgac
    16081 cgaaaaaacc gccgaacaac tgcccgacat cgacggcatt gccctctaca ccgcccaagc
    16141 aggcgtgaag aagcccgggc ataccgacct gacactgatt gccgtagccg ccggcagcac
    16201 cgtcggtgca gtcttcacga ccaaccgttt ctgtgccgcg cccgtccaca tcgccaaatc
    16261 gcaccttttc gacgaagacg gcgtgcgcgc cctcgtcatc aacacgggca acgccaacgc
    16321 gggtacgggc gcacagggca gaatcgatgc tttggcagtg tgtgccgccg ccgcccggca
    16381 aatcggctgc aaaccgaacc aggtgctgcc cttctccacc ggcgtgattc tcgaaccgct
    16441 gcccgcagac aaaatcatcg ccgccctgcc caaaatgcag cctgccttct ggaacgaagc
    16501 ggcacgcgcc atcatgacca ccgacaccgt gcccaaagcc gcctcgcgcg aaggcaaggt
    16561 cggcgacaaa cacaccgtcc gcgccacggg catcgccaaa ggctcgggca tgattcatcc
    16621 caatatggcg accatgctcg gtttcatcgc caccgatgcc aaagtttccc aacccgtcct
    16681 ccaactgatg acgcaggaaa tcgccgacga aaccttcaac accatcaccg ttgacggcga
    16741 caccagcacc aacgacagct tcgtcatcat cgccaccggc aaaaacagcc aaagcgaaat
    16801 cgacaacatc gccgacccgc gttacgccca actcaaagaa ttgttgtgca gcctcgcgct
    16861 cgaactcgcc caagccatcg tccgcgacgg cgaaggtgcg accaagttca tcaccgtccg
    16921 cgtcgaaaac gccaaaaccc gcgacgaagc ccgccaagcc gcctacgccg tggcacgttc
    16981 gccgctggtc aaaaccgcct ttttcgcctc cgaccccaac ctcggcaggc tgctcgccgc
    17041 catcggttat gccggcgttg ccgacctcga taccgacctc gtggaaatgt atctcgacga
    17101 tattttggtt gccgaacacg gcggacgcgc cgcaagctac accgaagcac aagggcaggc
    17161 ggtaatgtcg aaggccgaaa tcaccgtccg catcaagctg catcgcggac aagccgccgc
    17221 caccgtctat acctgcgacc tgtcgcacgg atacgtttcc atcaacgccg actaccgttc
    17281 ctgacccgac acggcttcag acggcataca taaaattccg tctgaaccgc cggacaacat
    17341 accatgacct ccacattccc ccgccgcctc gcccgcaaaa tccgccaaac ccgccgcctg
    17401 tcgcgcaaaa gcatcgcctt tctgttcctt ttggcaggtt cggcactcgt cgccctgacc
    17461 gcgctgtttt ttgcccatct tgccgatttt gcgctggaac tgaacgccaa actggttcaa
    17521 caatacccat ggttcgcgtg ggtcgcgctt cctttgggtt tgccgcttat tgcgtggctc
    17581 acacgcaaat tcgccccctt caccgccggc agcggcatcc cgcaggtcat cgcctcactg
    17641 tcgctgccct acggcgcaca gaaaacgcgg ctgatccgcc tcgggcagac actgctgaag
    17701 attccgctga cctttttggg tatgctgttc ggcgcgtcca tcggacgcga aggtccgtcc
    17761 gtgcaggtcg gcgcggcagt gatgggcgcg tggggcgcgt ggtgcaagaa acacggcttg
    17821 gcattcaaag ggatgcagga aaacgatttg atggcggcgg gcgcggcggg cggtttggca
    17881 gccgcgttca acgcgccgct ggcgggcgtg attttcgcca ttgaggaact cgggcgcggc
    17941 atcatgttgc gctgggagag gcaaattctt ttgggcgtgc tcgcctccgg tttcatacag
    18001 gtcgccattc agggcaacaa cccgtatttt tccggcttca acggcggcgt attggaacat
    18061 atctttctgt gggtcgcact gtccggcctg gtttgcggcg cggcgggcgg cctgttcgga
    18121 cgtttgctct atcgcggtgc ggcggcgttt gcaccgcgca agatacgcgg cttcatccgc
    18181 aaccgtccgc tgctgctggc ggcactgatg gggctgctgc tcgccctgct cggcacgttc
    18241 taccaaggca aaacctacgg caccggctac cacgaagccg cccaagccct gcacggcatc
    18301 tatgaagccc ccttcggact cgccgccgcc aaatggctcg ccaccgtatt cagctattgg
    18361 gcaggcgttc cgggcggcat tttcactccc tcgctgacca taggcgcggt tttgggcgag
    18421 catatcgccg ccatcgccga catatcgcag ggtgcaaaca tcatcgtcct catctgcatg
    18481 gcggcatttc tggcgggcgc gacacaatcc ccgattactt ccgccgtcgt cgtcatggaa
    18541 atgacgggcg gacaaagcct gctgttttgg atgctaattg cctgcatttt cgcctcgcag
    18601 gtttcgcgcc agttttcgcc gcgtccgttc taccacgcat cgggaatgcg cttccgccag
    18661 cgcgtgcttc aggaaaccac cgcccaaacc ggcaatgcgc ccgcaagacc gcaaacagca
    18721 aaacgggaat gccgtctgaa aattaaaacg cccccgatca aacgccggca gccgccttga
    18781 tttgaatacc gttccgccgc tgcttgaaac ttcaacaaca atgccgtctg aacgacagaa
    18841 tgcggttttc agacggcatt tccccatccc gatattgcct aaacaaaacc gaagcgtttg
    18901 ctataattgc actttttacc gcacacgcac gcccatgttt cccgatttct cccaaaccct
    18961 ctccaaagac cgccacttcc tgcaatccgc cttcaaaaat cccaacaaat acggcggctt
    19021 gtccaaagtc gaagaaaaat accgaaaatc gcacgaaatc tttttgaaac gtttggcagc
    19081 cttgcccaaa cccgaattcg acaacaccct gcccgttcac gaaaaacttg acgaaatcaa
    19141 aaaaaccgtt gccgaacatc aggtaacgat tatttgcggc gaaaccggtt cgggcaaaac
    19201 cacgcagttg cccaagattt gtttggaact cgggcgcggg gtagcgggtt tgatcgggca
    19261 tacccagccg cgccgtttgg cggcgcgttc ggtggcagag cggattgccg aagagctgaa
    19321 atccgaaatc ggcagcgcgg tcggctataa agtacgcttc accgaccaca cctcgcgcga
    19381 tgcctgcgtc aagctgatga ccgacggcat cctgctggcg gaaacgcaga ccgaccgtta
    19441 tctcgccgcc tacgacacga ttatcatcga cgaagcgcac gagcgcagcc tgaacatcga
    19501 cttccttttg ggctatttga aacaactcct gccgcgccgc cccgatttga aagtcatcat
    19561 cacctcggca acgatagacg cagaacgctt ctcccgacac ttcaacggcg cgcccgtttt
    19621 agaagtgagc ggacggacgt atcccgtcga aatcctctac cgaccgctga ccggcaaaga
    19681 cgaagacgac gcagaagtgg agttgaccga cgcgattgtc gatgccgccg atgaattggc
    19741 gcgatatggc gaaggcgata tcttggtatt cctgccgggc gaacgcgaaa tccgcgaagc
    19801 cgccgaagcc ctgcgcaaat ccacgctgcg ccgcaacgac gaaatcctgc ccctgttcgc
    19861 acgcctgtcg cacgccgaac agcacaaaat cttccacccc tccggcgcaa aacaacgcat
    19921 cgtgctggca accaacgtcg ccgaaacctc gcttaccgtg ccgggcatca aatacgtcat
    19981 cgacaccggt ctcgcacgcg tcaaacgcta ttccgcacgg gcgaaagtgg agcagcttca
    20041 tatcgaaaaa atctcccaag ccgccgcccg ccaacgctcc ggccgctgcg gacgcatctc
    20101 cgcaggcgta tgtatccgac tgttttcaga agaagatttc aacaaccgcc ccgaatttac
    20161 cgaccccgaa atcgcccgca gcaacctcgc cgccgtcatc ctgcgcatgg catcgctgaa
    20221 actcggcgac gtagcggcat tcccattttt agaaatgccc gattcgaggt atattaatga
    20281 tgggtttcag gtgttgctgg aattaggggc agtggaggcc gcctgaaaac aagtaggata
    20341 tacacttcta cctgacaaag accaacaaac gataaaacta cggcacatct agatgcccaa
    20401 cctacaacat tgcacacggt aaaaacatga aacacttact catcgatttt gaaaacgtcc
    20461 agccgcaaaa cttagacaaa ttactaaccg aaaataccca tatttggcta tttataggtg
    20521 tattacacaa aatgttacct attagtctgg tgcaatccct actacgtttc ggcgaacgtg
    20581 tccatcttgt ccagttacaa aaaacgggga aaaacgcatt ggatttttac ctgtcctatt
    20641 acctcggaca aattaccgcc acagacccca atgcccaaat cggcatactc tcgcgtgatg
    20701 gaggatacga tgttctggtc gaacatattt tgaaaaacca ccaagcgaag ggtatcgtgc
    20761 gcctagccaa tataaatgaa gtacaacatc agaaaattac taccgaaccg ccgtcagcat
    20821 tgctggaaaa cactcctcag cctgaaacca cactcaaacc acagcaacca ttaacttcct
    20881 atttccaagc agccctaact gcactgcgcc gccccgacgc tttccgcccc tgccgcctgc
    20941 ataacctgcg acaaaatctg cgtaagcata ttttgagtga tttgtttaaa gaaaaaaccg
    21001 atgaagaatg cgaaataacc actgctaacg ttatcaataa actcaaagca caaaacttca
    21061 tcagcattga tgaacaggaa accgtttcct accatctcag tgataatgat ttgttacaaa
    21121 gaatccaacg ccatatttta agccaacgtc ccaaaaccta cgctgatttt caagccgtcg
    21181 tgcaaaaccg agcagatgca cttcacttaa cagtcggtac caacgacatt caatcctttg
    21241 cgcgacattt gcgcgaccaa aacctgatcc gccaaaacaa tgggaaaatt gaatatgcac
    21301 cgtttactga acctaaacca cagccaacac ccaagcagcc taaaaaaacc gcatgggaac
    21361 ctgatgaaat tatttggaaa aaagtgattg ccgcgttatc gttaaagaac cgtcctaata
    21421 aaaccaaaac tttacgcaat acaatccagg cactcacaaa atccaatgca caagaaactg
    21481 acaaactgct acaacattta caagataccc aagtcctacg tattgacggt actaaaattg
    21541 tgtatatcaa gtaggatggg catttatgcc tgacctacaa aaccaaaaac aacctaacac
    21601 ccttaatcaa ctccacaaac ccttttcaga caacctcgtt ttttgaaaaa caatctgtaa
    21661 acagataact gctgaagaat accgttgccg agccccaaaa cctgcctgca acttttattg
    21721 tgaactgccc attatgaaaa aatccctttt cgttctcttt ctctattcat ctctacttac
    21781 cgccagcgaa atcgcctatc gctttgtatt cggaattgaa accttaccgg ctgcaaaaat
    21841 ggcagaaacg tttgcgctga catttgtgat tgctgcgctg tatctgtttg cgcgttataa
    21901 aacttcgcgg ctgctgattg cgctgttttt cgcgttcagc attattgcca acaatgtaca
    21961 ttatgcggtt tatcaaagtt ggatgacggg tattaactat tggctgatgc tgaaagaggt
    22021 taccgaagtc ggcagtgcgg gcgcgtcgat gttggataag ttgtggctgc ctgcgttgtg
    22081 gggcgtggcg gaagtcgtgc tgttttgtag ccttgccaag ttccgccata agacgcattt
    22141 ttctgccgat atattgtttg ccttcctaat gctgatgatt ttcgtgcgtt cgttcgacac
    22201 gaaacaagag cacggtattt cgcccaaacc gacatacagc cgcatcaaag ccaattattt
    22261 cagcttcggt tattttgtcg gacgcgtgtt gccgtatcag ttgtttgatt taagcaagat
    22321 tcctgtgttc aaacagcctg ctccaagcag aatcgggcaa ggcagtattc aaaatatcgt
    22381 cctgattatg ggcgaaagcg aaagcgcggc gcatttgaaa ttgtttggct acgggcgcga
    22441 aacttcgccg tttttaaccc ggctttcgca agccgatttt aagccg
//
LOCUS       DAWFBO010000013        22443 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFBO010000013 DAWFBO010000000
VERSION     DAWFBO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.013"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            43..606
                     /locus_tag="WGO87_000361"
     CDS             43..606
                     /locus_tag="WGO87_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0391774.1"
                     /translation="MRHLENNGYANVAGLERILAVKTDNYKEKENLLHEIFSKSRIGD
                     TELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGNAGSGRKQLLDLV
                     RRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGKSGTRLTDEIADGIHIYTCFS
                     RADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            643..1404
                     /locus_tag="WGO87_000362"
     CDS             643..1404
                     /locus_tag="WGO87_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0391775.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            1459..1800
                     /gene="fdx"
                     /locus_tag="WGO87_000363"
     CDS             1459..1800
                     /gene="fdx"
                     /locus_tag="WGO87_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0391776.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(2087..2674)
                     /locus_tag="WGO87_000364"
     CDS             complement(2087..2674)
                     /locus_tag="WGO87_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0391777.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            2887..3084
                     /gene="iscX"
                     /locus_tag="WGO87_000365"
     CDS             2887..3084
                     /gene="iscX"
                     /locus_tag="WGO87_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0391778.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(3152..3553)
                     /locus_tag="WGO87_000366"
     CDS             complement(3152..3553)
                     /locus_tag="WGO87_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0391779.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            3764..4723
                     /locus_tag="WGO87_000367"
     CDS             3764..4723
                     /locus_tag="WGO87_000367"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0391780.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4791..6101
                     /gene="tilS"
                     /locus_tag="WGO87_000368"
     CDS             4791..6101
                     /gene="tilS"
                     /locus_tag="WGO87_000368"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0391781.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            6175..6960
                     /locus_tag="WGO87_000369"
     CDS             6175..6960
                     /locus_tag="WGO87_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0391782.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEGKMPSEEVRRLRAVVPEGKVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGSADVWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLASYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            6970..7167
                     /locus_tag="WGO87_000370"
     CDS             6970..7167
                     /locus_tag="WGO87_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391783.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            7429..7782
                     /locus_tag="WGO87_000371"
     CDS             7429..7782
                     /locus_tag="WGO87_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391784.1"
                     /translation="MKNCKFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            7794..8099
                     /locus_tag="WGO87_000372"
     CDS             7794..8099
                     /locus_tag="WGO87_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391785.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDLVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            8161..9537
                     /gene="mpl"
                     /locus_tag="WGO87_000373"
     CDS             8161..9537
                     /gene="mpl"
                     /locus_tag="WGO87_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0391786.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVYEGFDTAQLDEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLHVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            9744..10796
                     /gene="bioB"
                     /locus_tag="WGO87_000374"
     CDS             9744..10796
                     /gene="bioB"
                     /locus_tag="WGO87_000374"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0391787.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(10874..11509)
                     /locus_tag="WGO87_000375"
     CDS             complement(10874..11509)
                     /locus_tag="WGO87_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0391788.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGSHVTRAVELYRDRFISYSGGNFATYGAIKTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQIGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            11832..12092
                     /locus_tag="WGO87_000376"
     CDS             11832..12092
                     /locus_tag="WGO87_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0391789.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(12155..14014)
                     /gene="ilvD"
                     /locus_tag="WGO87_000377"
     CDS             complement(12155..14014)
                     /gene="ilvD"
                     /locus_tag="WGO87_000377"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0391790.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVEHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(14187..15956)
                     /gene="cysI"
                     /locus_tag="WGO87_000378"
     CDS             complement(14187..15956)
                     /gene="cysI"
                     /locus_tag="WGO87_000378"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0391791.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(15983..17797)
                     /locus_tag="WGO87_000379"
     CDS             complement(15983..17797)
                     /locus_tag="WGO87_000379"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0391792.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAANAWTDNIAALLKEEAAKNRATP
                     APQTTPPAGLQTASEGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(17794..19080)
                     /locus_tag="WGO87_000380"
     CDS             complement(17794..19080)
                     /locus_tag="WGO87_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0391793.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(19119..20042)
                     /gene="cysD"
                     /locus_tag="WGO87_000381"
     CDS             complement(19119..20042)
                     /gene="cysD"
                     /locus_tag="WGO87_000381"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0391794.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(20080..20820)
                     /locus_tag="WGO87_000382"
     CDS             complement(20080..20820)
                     /locus_tag="WGO87_000382"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0391795.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIAGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(20831..22273)
                     /gene="cobA"
                     /locus_tag="WGO87_000383"
     CDS             complement(20831..22273)
                     /gene="cobA"
                     /locus_tag="WGO87_000383"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0391796.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQ
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFQLAESCQKPVNV
                     VDDRDYCSFIFPSIINRNPIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAENAPKPALIVIGEVVGLHEKL
                     AWFGENGEGENRVGQAYPALGGLNAGQWAA"
BASE COUNT         4860 a         5821 c         6444 g         5318 t
ORIGIN      
        1 tataaaatcg gcatttcgga tgtaagcaat tttgaaggca gaatgcgcca tttggaaaac
       61 aacggctatg cgaacgttgc cggattggaa cgcatcctcg ccgtcaaaac cgacaattac
      121 aaagaaaaag aaaacctgct ccatgaaatt ttcagcaaaa gcaggatagg cgataccgaa
      181 ttgttcgccg tggacgaaaa ccttgtaaaa cgtttgtttt tatcgcttcg cggcgaaatc
      241 gtgtttccga aaaacgaaac ggcggaatcg gaatttgaaa aaagcgtcca cgaacgcagg
      301 caggaaggga atgccgggtc cggccgcaaa caactgcttg atttggtacg gcgcggacac
      361 cgggaatacc cttacgcgct gccccggctt ttggcgggcg cggcatccta caagccgaaa
      421 aaatcgaaaa tccgcctttt taaagaagca tatttcggca aaagcggcac gaggctgacc
      481 gacgaaattg cagacggcat ccatatttac acctgttttt cgcgggcgga tttggaaaaa
      541 gcctattccg aatatttgga acttttcaaa tccgaatcgg atgccgaagg cagaaagccg
      601 cagtaaggtg caaacagata ccgtacacgt tgaggagcag atatgatggg cgattccgtc
      661 atttattatg tagaacaggc agacaaaccg gtaaaccgtg ccggcgaacg cgcccgtaaa
      721 acattcaaat atttttggcg cgagcttttt tgggaacgcc gccgcatcat tcccgcctta
      781 gattttgcca tggtcaaagc cccttttttc caagacggcg aagacggcga aatttgcgaa
      841 catatgtgga tcgacgatat ttattttgac ggtctttaca tttatggcgt gctgaacaat
      901 gaacccggcg aactgaccaa tgtcgaacaa ggcgaaagcg tttgcgttcc ggttgacgac
      961 atcagcgact ggatgttcgt gtgcaacggc attccctacg gcggctttac catacaggca
     1021 atgcgtggac agatgacgga agaggagcgt accgaacacg atgccgcatg gggaatcgat
     1081 ttcggcgatc ccgggcaggt attgctggtg tatgaagaaa aagaacatcc cgaaaatctg
     1141 gaagagcatc cgatgtgccg gaactgtatt gacgattttc ggcaacagtt gtcccaaaat
     1201 ccggattttc tgcatgagca ggacgaagac ggctatacgc cgcttcatca tgaagccatc
     1261 gcaggaaatg cacttatggt tcaagccatg cttgaatacg gcgcaaatcc tgcctcaaag
     1321 acatcggaag gctataccgc cctcgatttt gcccgcctgg cgggctggca aaatgttgcc
     1381 gacctgctcg aaccgcgaca ttaggcggac agttttccga aaacgaacac aaacactttt
     1441 tacagaaaga caataaaaat gcccaaaatc accgtacttc cacacacgac attatgcccc
     1501 gaaggcgcgg ttatcgaaga cgcgcccgaa ggcaaaaccg tccttgacgt gctgctcgac
     1561 catgatatcg aagtcgatca cgcctgcgaa aaatcctgcg cctgcacaac ctgccacgtg
     1621 attatccgca aaggtttcga cagcctggaa gagccgaccg aattggaaga agacctgctc
     1681 gatcaggctt ggggtttgga agccgattcg cgcctgagtt gtcaggcggt tgtcgccggc
     1741 gaggatttga ttgtggaaat ccccaaatac accatcaacc acgcgcgaga agaacactga
     1801 aaacaggccg tctgaagccg gcacgcttca gacggcattg ttgcgcggat aaggcgcaat
     1861 cgcccgaaaa caggcgttcg tacaggcgga actttcgatt ctatagtgaa ttaacaaaaa
     1921 tcaggacaag gcggcgagcc gcagacagta ccaatagtac ggcaaggcga ggcaacgctg
     1981 taccggttta aatttaattc actatatatt gatttttatc ggttttctga cggaacggat
     2041 tggtgcggca tccgaggcgg attactcggg cgcgatgcac gggtatttat cggttttgca
     2101 gccggaaaaa ccgccggcgg gttactttca gggcggcggt cgggcggtat gccggatagc
     2161 gttccacttt cgcttctata ttgattttga ttggttttct gacggaatga tccgatgcgg
     2221 catccgggac ggcttgtgtt tcttcctgcc cgcctgccgg atattcctgt acttgcacga
     2281 aaccgaaaga ggtggcggtg cggcggacaa gctcgagata gcgtccttca agctccggac
     2341 aggcggcgga cacgctttct gccgtaaccg tgaagccgcc gcccgacccg gcaaggcgtt
     2401 ccgcaatctg cgtgtagatg cgccaacgtt gggcgcgcaa cattgccgca tcgcaaccgg
     2461 cagccgcttt atccaaggca atcagaccgc gcctgctccg gtagtgaagg caaaccgtta
     2521 ctctggagag gatatgttgc ccgtccaata tttgatacag tttgcgtatc agcagaatca
     2581 ggcggtcaaa ctcctccatc tccgacaggg aatcaggatg gtcggaagcg gtataaacca
     2641 gtttggacaa ttttgcggca aacatattgt tcatcaatct tccttgtcgg ttgaaacccc
     2701 gctcttcggg gcggtagaat cagatttgtt tgggagggac aactcctccc agatcaggac
     2761 gacacatagg ctggtgcttg atgtgttgtc cggcgagttg aaacattcag ccatcctcaa
     2821 ggtgcggcag ttttgccgaa acatattcta cacggcttca atgccggacg ataaaaggaa
     2881 attcatatga aatggaccga cacccggcgt atcgccgaag aactctatga cctgcacggc
     2941 gaaaccatcg atcccagaac cgtgcgcttt acccaactgc gcgacctgat tatggcattg
     3001 cccgaatttg acgacgaccc cgcccgttgc ggcgaacgca tcctcgaagc cgtgcagcag
     3061 gcatggatag acgaggcgga ataagtttcg ggaatgccgt ctgaaatgcg gcggtacgcg
     3121 gttcgtgctt ctgtttgcag cgggaatggt tttaccagtc tccttttttc agcctgtcca
     3181 gttggcggcg gtcgcgcttg gtcggtctgc cgtcgggata ggcggaagtg atgcggctga
     3241 attggtcgag ctgtttgcgc tcttccctca atgttgccgt tttcgcgtcc tcttcataca
     3301 gaagccgcgc ctcggatgcc gggcggcgtt ggtggttcaa acctttaacc ttgattttat
     3361 agggaaggga attgagcgtc aggtcgataa tatcgccgat gtctatggtt ttactgtttt
     3421 tgaccttcga gccgtttact tgaaccctac ccagttcgat gtgcttttgc gcaagggaac
     3481 gggtcttgaa aaaacgtgcc gcccaaagcc atttgtccag ccgcatggcg gaagaatcgt
     3541 gcttgtcttt catacgattt tgtttgaaat aattgaattt gtttcaagtt tagcataaga
     3601 tacgccgcct ataactagta tatatgcact aatccactgt tttccatgct gtccgaacac
     3661 aaaaagaggg tgtggaaaag ccgttttgga caataaatta actgcggaat atgcacaaat
     3721 agcgtatgat agcggcagaa tctgttgatg agagcttcat tctatgaaac ctgttttttt
     3781 ggattttgaa caacccatag ccgaactgac caacaaaatc gatgagctgc gtttcgtcca
     3841 agacgagtct gccgtcgata tttcggacga aatacaccgt ttgcagaaaa aaagcaacga
     3901 cctgaccaaa tcgattttca gcaaacttac gcccgcccag gtttcacagg tttcccggca
     3961 tccgcagcgt ccctatactt tggattacat tgaggcactg tttactgatt ttgaagagct
     4021 gcacggcgac cgccactttg ccgacgatca tgcgattgtc ggcggattgg cgcgtttcaa
     4081 cggacaaagc gtgatggtcg tcgggcatca gaaagggcgc gacaccaaag aaaaaatccg
     4141 ccgcaacttc ggtatgccgc gccccgaggg ctaccgcaaa gccctgcgcc tgatgaaaac
     4201 ggcagaaaaa ttcggcttgc ccgtcatgac ctttatcgat acaccgggcg cgtatcccgg
     4261 catcggcgcg gaagaacgcg ggcagtcgga agccatcggc aaaaacctgt acgaactgac
     4321 acgcctgcgc gttcctgttt tgtgtaccgt catcggcgaa ggcggttcgg gcggtgcgtt
     4381 ggcggtcgcc gtgggcgatt acgtcaatat gctgcaatac tcgacctatt ccgttatttc
     4441 ccccgaaggc tgcgcttcga ttttgtggaa aaccgccgaa aaggcggcgg atgcggctca
     4501 ggctttgggc attactgctg accgcctgca aaagctggac ttggtcgata ccgtcatcaa
     4561 agaaccattg ggcggcgcgc atcgggattt tgggcaaacc atgaaaaacg tgaaagccgt
     4621 tttggaaaaa caactgcacg aagcgcaaag catcccgctt gccgatttgc tttcgcgccg
     4681 tttcgaccgc attatggctt acggcaaatt ttcggaacaa taattcaggt agaacaagca
     4741 gcaagcagtt tgtctgaaac tgcttgcttt ttctttatcg ggacggaacc gtgctgactt
     4801 tagatgcgtt tgagcaatgc ttgaaggatt gttttcctca aggtctgaat ggaaaaaaaa
     4861 cagcggtggc attaagcggc ggcttggatt ccgtcgtttt gctgcatctg cttgtccgcg
     4921 ccggaaaaaa gggcggtttt attccggatg cattgcatat ccatcacggc ttgagtcccc
     4981 gtgccgacga ttgggcagat ttctgccaaa actattgcga tatgctcggg gtggggctgg
     5041 aaacggttaa ggtctgcgtg gaaaaaaacg gtttgggcat cgaggcggcg gcgcggcaaa
     5101 agcgttatgc cgcgtttgcc gaaaaaggct ttgacgtttt ggcgttggcg caccacaggg
     5161 acgatcaaat cgaaaccttt atgctggcag tcgctcgcgg cggcggtttg cgcgctttgg
     5221 cggctatgcc caccgtccgc ccttttgggg aaaaaggcat catctggcgg cctttgctgc
     5281 ctttttcgcg ccaagacata tgggattatg cccaaaaaca cggtttgccg aatatcgagg
     5341 acgaaagcaa tagcgatacg gcttatttgc gaaaccgctt ccggcaccgt attttgcccg
     5401 aactttcggc gcagattccc catttcgggc ggcatgtact gaacaatgtc cgcgctttgc
     5461 aggaagattt ggctttgttg gacgaaatca tcggtcagga ctgccgttgg gtctgtgagg
     5521 aaggcagttt cgatacggtg cgttggcagg cgttttcccc acgccggaaa acccatattt
     5581 tgcggtattt tttgaaggaa aacggcatcc ccatgccgaa tcagaatgcc cttgccgaca
     5641 ttgcccgggt tttgacggag gcaaaaaccg gacgttggaa cttgcaaggc tttgaattgc
     5701 atcattatgc aggcaggctg tttgtgttcc gactggaaaa aacggataaa ctgcggtttt
     5761 tgaaagacag gcagataagc ggaaatttaa gggaaatatt gacggggcag ggatttgttt
     5821 taaaacgtca tccgtttggg cttcctgagc atcttttgga gcaggacgga attttgagga
     5881 cggtagcggt atcggatacg ttggctgtcg gcggcatcca taaggatgtg aaaaaaatcc
     5941 ttcaggggaa acgggttttg cctgtcctgc gcccaatttg gccgcttgtt gccgacagcg
     6001 gaaaccgtcc attggcgttg gcaaactgtt gtgcggattt ccaatactcg gtttcagacg
     6061 gcattttgcc cgtccatcct gactttccca ttttattttg ataatatcgc aaacagattt
     6121 cggcggcgtt cagacgggta ttgtccggtt gcatatttct aaaaggcttg tgaagtgaaa
     6181 cacatcagtt cgaccaataa tgaacacatc cggcacctgc accgcctgtt gtcgcaagga
     6241 aagttcagac ggcaatacgc ccaaaccgtt ttggagggcg tgcacctgct tcaggttttc
     6301 ctgcaatccg gcagaaaacc ggtcggggta tatattcccg agggcaaaat gccgtctgaa
     6361 gaagtccgca ggctgagggc ggttgtgccg gaaggcaagg ttttttccgt ttcagacggc
     6421 atattgaaaa aaatcagcag cttgacttgt gcggatgatg tgcttgcgct gattgatatt
     6481 ccggatgcgg gtgctttgcc tgccggcggc gactgcgtgg ttttggacgg cgtgcaagac
     6541 ccgggcaatg tcggcacggt gttgcggagc gcggcggcgg caggggtagg tacggtcgtt
     6601 ttgggcaggg gttcagcgga tgtttggtcg cccaaggtat tgcgcgcggg gatgggcgcg
     6661 catttcttgt tggacattta ttcgcaggcg gatttggaaa tatggttggc gagctataaa
     6721 ggccgtgtgt ttgccaccgc cttgcgagag gaaaagcagg cggttttgta cggcgaagat
     6781 ttgtgcgaac cgacagcctg ggtgtttggc aacgaaggcg cgggggtcgg taaagcggtt
     6841 ttggacaggg cggacaagtg tgtcaggatt ccgatgcacg atgcaaccga gtctttaaat
     6901 gtcgcgatgg cggcgacaat ctgcctgttt gaacagatgc ggcagcggtc ggcgtattga
     6961 ggaaaagaaa tgccgtctga aaaaatctat tacggcgtat tgattttctt atgtatcgct
     7021 tctatgctgc tgtcgccgtt tttttatgcg ggtgctttga agcccaagaa ggcggcattg
     7081 cggaaggacg ggcagtggaa actcatctta ttgtccaata ccgtggcggc ggcggttttg
     7141 ttttggatgt ggtggaaatg gttttgacag atattgctca aaattgggtt aatggaatcc
     7201 gaacaaataa ataatttggt aaaaaatttg ttaaatcaat ggattaaagt ttttcgggaa
     7261 acaaaacagc tctaagcaaa tagggcgttt gtcggtaaat acggaagagt tgcggcatta
     7321 tcgggcatct ttaacaagta gtgccggctt ggcaggcaat tggtttttat gggcagcttg
     7381 caaaatcgcg gatataaaat tgcgaatcgg ttaaagcgtg aggataccat gaaaaattgc
     7441 aaattttttt atgatccgac aagggcaatc tatgatagcg gtgcagatta tttaaccaga
     7501 gaaaaacata gattagtcgt aattgcaaat agtgcttggg ggctattgct taatttatct
     7561 tgttattatg acgaggtttt ggaaaagcgg aaaataccgt tcggcaaaca ggaaattgat
     7621 gacgatatgg acaaagtgtc cgcccttaag cggaagttta aagatatttc tgaaatcaaa
     7681 gtaggggatg gttgggaata cccgttcaat tatgagcagg gaatgaaaga attagatgaa
     7741 gtgctattga aatacattcc cttttttgaa gaagaacgat aaaggaggtt gatatgcgcg
     7801 tatctaaaat aattggaagt atgttgcttg ttacagcggt tcagaccgta ttttcggcaa
     7861 atgtttacgc gtgccgccat aatggtaaaa ccagttacag ccaaactccg ggaaaacatt
     7921 gtaccaacgc gggtttgggg cgggacctgg tgtacagttc ggttagaccg gcagtaaaag
     7981 acagggcgga agacgcaggg gtcggcgatt attcggacac ggtgagggac gaacacgtcc
     8041 aaaatccgag agagaatacg cataaggatg cttcgaatac cggtgcaaag acgcattgat
     8101 tgaaaccgaa tcagccatga cggcgttgaa taatagaatt tgaacgatta gggaactctg
     8161 atgaaacaca tccacattat cggtatcggc ggcacgttta tgggtgggat tgccgccatt
     8221 gccaaagaag cagggtttga agtcagcggt tgcgatgcga agatgtatcc gccgatgagc
     8281 acccagctcg aagccttggg cataggcgtg tatgaaggct tcgacaccgc gcagttggac
     8341 gaatttaaag ccgacgttta cgttatcggc aatgtcgcca agcgcgggat ggatgtggtt
     8401 gaagcgattt tgaaccgtgg gctgccttat atttccggcc cgcaatggct ggctgaaaac
     8461 gtgctgcacc atcattgggt actcggcgtg gcggggacgc acggcaaaac gaccaccgcg
     8521 tctatgctcg cgtgggtttt ggaatatgcc ggactcgcac cgggcttcct tatcggcggc
     8581 gtaccggaaa acttcagcgt ttccgcccgc ctgccgcaaa cgccgcgcca agacccgaac
     8641 agccaatcgc cgtttttcgt cattgaagcc gacgaatacg acaccgcgtt tttcgacaaa
     8701 cgctccaaat tcgtgcatta ccgtccgcgt accgccgtgt tgaacaatct ggaattcgac
     8761 cacgccgaca tcttcgccga tttgggcgcg atacagaccc agttccacca cctcgtgcgt
     8821 accgtgccgt ctgaaggcct catcgtctgc aacggacggc agcaaagcct gcaagacact
     8881 ttggacaaag gctgctggac gccggtggaa aaattcggca cggaacacgg ctggcaggcc
     8941 ggcgaagcca atgccgatgg ctcgttcgac gtgttgcttg acggcaaaaa agccggacac
     9001 gtcgcttgga gtttgatggg cggacacaac cgcatgaacg cgctcgccgt catcgccgcc
     9061 gcgcgtcatg ccggagtcga cattcagacg gcctgcgaag ccttgagcac gtttaaaaac
     9121 gtcaaacgcc gcatggaaat caaaggcacg gcaaacggca tcaccgttta cgacgacttc
     9181 gcccaccacc cgaccgccat cgaaaccacg attcaaggtt tgcgccaacg cgtcggcggc
     9241 gcgcgcatcc tcgccgtcct cgaaccgcgt tccaacacga tgaagctggg cacgatgaag
     9301 tccgccctgc ctgtaagcct caaagaagcc gaccaagtgt tctgctacgc cggcggcgtg
     9361 gactgggacg tcgccgaagc cctcgcgcct ttgggcggca ggctgcacgt cggcaaagac
     9421 ttcgatgcct tcgttgccga aatcgtgaaa aacgccgaag taggcgacca tattttggtg
     9481 atgagcaacg gcggtttcgg cggaatacac ggaaagctgc tggaagcttt gagatagccc
     9541 gggcgatgcc gtctgaaagc ccttcagacg gcatcgcccg gctgcgcggc acaaaggcgg
     9601 aaaaaccgtt tgccccgtat tttcaaacgc gttacacttg ccgccgctgt tttcagccat
     9661 ttgattaccc gcaaccgccg tcattgcgcc ggcggtttgc ctgtcagcgt cattgcgccg
     9721 ctgtaaatac gaaagaacac attatgaccg tatcccccgt cgccttgcgc cgtaagaccg
     9781 agtgcaagcc tcatcccacc gcgcgctatt ggaaaaaatg cgatgtcgaa gccctgttcg
     9841 gacttccctt cctcgacctc atttaccaag ccgccgaaat ccaccgccaa aatttcaacc
     9901 cgcgcgaaat ccagctttcc acgctgttgt ccatcaaaac cggcggttgt cccgaagact
     9961 gcgcctattg tccgcaatcg gcgcaccaca ataccaatct gggcaaagag cagatgatgg
    10021 atgtggatga aatcgtcgaa aaagccaaaa tcgccaaatc gcgcggcgca agccggtttt
    10081 gtatgggcgc ggcgtggcgc ggccctaaac ccaaagacgt ggagacggtt tccgcaatca
    10141 tcaaagccgt caagggcttg ggtatggaaa cctgcggcac gttcggtatg ctcgaagaag
    10201 gtatggcgga agacttgaaa gaggcgggct tggattatta caaccacaac ctcgacaccg
    10261 accccgaccg ctacaacgac atcatccaca cccgccaaca cgaagaccga atggacacct
    10321 tgggcaaagt ccgcaacgcc ggtttgaaag tctgctgcgg cggcatcgtc gggatgaacg
    10381 aaacccgcgc cgaacgtgcc gggctgattg ccagtctcgc caatctcgac ccgcagcccg
    10441 aaagcgtgcc gattaaccgg ttggtcaaag tggaaggcac gccgcttgcc gatgccgaag
    10501 atttggactg gacggaattt gtccgcacca tcgccgtggc gcggattacg atgccgcaaa
    10561 gttatgtccg gctgtcggca gggcgcagca atatgccgga agcaatgcag gcgatgtgct
    10621 ttatggcggg cgcgaactcg attttttacg gcgacaagct gctgaccacg ggcaatcctg
    10681 atgaggacgg cgacagaatc ctgatggaaa agctcaacct gtatcccttg cagtttgaac
    10741 cggaaggcga ggtcgccgaa gtggaaaaag cctctgggat taaagtggat tattgacgat
    10801 tgaaaaatgc cgtctgaaac ccggaaaaag gctttcagac ggcatttgtc cggacggcat
    10861 ttccaatatc tttttaccgg cgcgtgatgc tgccgtcggg cgagacatcc agcccgttcc
    10921 ccttggggaa atcgctgcgg ttcagataaa taatccgccc gataacggtt ttctcggggt
    10981 cgattttggg gattttatcg ccgatttgag tgatggggat aatgttgccg gaaacgaacc
    11041 gcccctgttt gtcggtgata attttgaaaa tcggcgcaat gccgctgatg ccggaggttt
    11101 tgattgcgcc gtaggtggca aagttgccgc cgctgtagga gatgaagcgg tcgcggtaaa
    11161 gttcgacggc gcgagtaacg tgcgacccct gcccgaatac gacatccgcg ccggaatcga
    11221 cggcaagccg tgcaaactcg acgacgttgc ccctgttttc cccatagaag atttcggtat
    11281 cgaacggcag gtgttccgcc tgtttccctt ccgcgccgcc gtggaacatc acaatgacga
    11341 tgtcggcttt ctgtttggtt ttggtaatcc gttttctaac tttggcataa tcgttcagtt
    11401 tgacggcggc aaggttgggg gcgaaggaga cgaagccgga tcttacgccg tttttcttca
    11461 ggatggcggt ttcaaacctg ttttcgatgc ccgaatattt gatgttcaat tcgtcaaggt
    11521 tcgcccttta tgccgtttcc gcaccgcaaa ccgccggggt caagccctcg gcgcatcctg
    11581 ccggaacgga atccccgtac tgccgattga cggttcaaac cccgcgcccg tttcaaatac
    11641 cggcgatgtg gacggacagg atgcgcctga cgaaaagaca gccgataccg tttccattat
    11701 cggcgtgggc gacattatgc tcggcagcaa ttatccggtc gattacctgc ccgataccaa
    11761 tattctgaaa aacgtcgaat ctgccttgca agacgcggac attaccgtcg gcaacctcga
    11821 aggcacgctg tttgacgaag gcggtacgcc gaaaaaatgt gcaacccccc aaaatatgct
    11881 atgcattccg aacgccctcc gcatacgggc aataccttgc cgacgcggga ttcgactacc
    11941 tcagcttcgc caacaaccac agcaacgact tcggcgcgca aggcatcacg gcaacggcgg
    12001 cgagcggcag ctcttttaca tactcgatcg cgctaaagcc gctgccgata acgaggccaa
    12061 aattgcggaa aataccgcca tcgcccagat aaatttgtcc atcatcagac ctttactgtt
    12121 cagacgagac agcatttgcc gcacgttttg gggcttatct ttcgatttgc gctacgtcgc
    12181 gcaccgcgcc tttgtcggcg gaagtcgcca tcgcgccgta agctcttaat gcggcggaga
    12241 cgtagcggtc gcggttttta ggcttccatg ctttgctgcc gcgcgcttcc atttcggcac
    12301 ggcgtgcggc aagctcttca tcggaaatgg caaggtggat gctgcggttg gggatgtcga
    12361 tttcgacggt atcgccttcg tgtaccaaac cgatcgcgcc accttccgcc gcttcgggcg
    12421 aggcgtgtcc gatggacaaa cctgatgtgc cgccggagaa gcgtccgtcg gttaagagag
    12481 cgcaggcttt gccgaggcct ttagatttca ggtaggaagt cggatacagc atttcctgca
    12541 taccggggcc gcctttcggg ccttcgtagc ggatgatgac gatgtcgccg gcgacgattt
    12601 ggttgcccaa aatgccttct actgcgtctt cttggctttc aaacacgcgg gcgcggccgg
    12661 tgaatttgag gatgctctcg tccacgcctg cggttttcac tacgcaaccg cgctcggcga
    12721 tgttgccgaa caagaccgcc aaaccgccgt cttgcgagta ggcgtgttcg acatcgcgga
    12781 tacagccttt ttcgcggtcg aggtcgaggg ttttccacat gcggttttgc gagaacgctt
    12841 gggtggtgcg cacgccgccg ggcgcggctt tgaagcgttc gatggcgcgg gtgttttcag
    12901 ggttggttac gtcccatttt tcaatcgcgt ctttcagcgt cggtgcgtgg atagtgtgca
    12961 cgtcggtgtg cagcttgccc gctttgtcca attctttcag gatggcgaag atgccgccgg
    13021 ctcggtgcac gtcttccatg tagtagtcgt ggttgttggg tgcggttttg cagatgcagg
    13081 gcacaacgcg gcttaagcgg tcgatgtctg ccattttgaa atccacgccc gcttcgttgg
    13141 caacggcgag taaatgcaaa atagtgttgg tgctgccgcc catcgcaata tccatcgtca
    13201 tagcgttttc aaacgctttt ttggtggcaa tgctgcgcgg taacacggtt tcatcgtttt
    13261 gctcgtaata gcgtttggtg atttcgacaa tcatacggcc ggcttcgagg aacaattctt
    13321 tgcggccggc gtgggtcgcc aaatacgaac cgttgccggg tagggacaga ccgagtgctt
    13381 cggtcaggca gttcatcgag tttgccgtaa acatacccga acacgagccg caggtcgggc
    13441 aggcgttttg ttcgacttcc tcgacttgcc ggttgctgac attgtcgtcc gccgattcaa
    13501 tcatcgcgtc aatcaagtcc aagcggcgtt ctggttggat gtttgccacg ccgataactt
    13561 tacctgcttc catcgggccg ccggagacga agatggtggg gatgttcagg cgcatcgcgg
    13621 caatcagcat gcccggggtg attttgtcgc agttggaaat gcacaccagc gcgtcggcgc
    13681 agtgggcgtt gaccatatat tcgatagagt cggcaatcaa atcgcggctg ggcagggaat
    13741 acagcatacc gctgtgtccc atagcgatgc cgtcgtcgat ggcgatggtg ttgaattctt
    13801 tggcgatggc gccggctttt tcgatttcgc gggcaaccag ctgacccata ttgtgcaggt
    13861 ggacatgacc tggcacgaat tgggtgaagg agttggcaac ggcgatgatg ggcttgccga
    13921 agtcggtttc catcacgccg gtggcgcgcc acaatgcacg tgcgcccgcc atattgcggc
    13981 cgtgggtgga ggttttggag cggtattcag gcatagtgtg tttccttgtg cctataccgt
    14041 ctgaaagaca gggctgtttc agacggtatc gggtacggtt ttttagagtg ggaaaagagg
    14101 gtattttata ccaagtatcg gaattttgcg ggattgaaac ggcgtgcggc aaaaaagaaa
    14161 atccccgcag gaatgcgggg acgggttcag gcgcgggcaa tcgcgacggc tttggatgcg
    14221 tcccaaaaat caacgggtgc atttaatacg ggtttgacga tgcccgtccg tatggcgaaa
    14281 tcgccgaaac cttcgccgat attgcgttct gccgcccatt tgccgatcag gtcgtccaat
    14341 tcggcaagga tttcgggcag ggtgatgttt tctttgtaaa gacgggggat gcgtacgcct
    14401 tcgcggtcgc cgccgatgtg gaggttgtag cgtccgacgg ctttgccgac cagtccgatt
    14461 tccgccaaca tcgcccgtcc gcagccgttc gggcagccgg taatgcgggt aacgatgtag
    14521 tcgtccgacg tgccgtgttt cgccataatc ttatccagct cgccgatgaa gtccggcagc
    14581 acgcgttcgg cttccgccat tgccagcggg caggtcggaa aggaaacgca ggacatcgca
    14641 ttttcacgca gcttgctgac atcgttgcgg attaatccgt atgttcgggc aaattcttcg
    14701 atttttgctt tgtctgcttc ggcgacattt gccacgatga ggttttggtt ggcggtgatg
    14761 cggaaatcgc ctttgtggat tttggcgatt tccaacacgc cggtcagaag ctgtttcccg
    14821 ccttcgtcaa ccaaacgccc gctttcgatg aaaagggtta aatgccagtt gccgtctatg
    14881 cctttcaccc agccgatgcg gtcgccgcgc ccggtaaatt tgaacgggcg tacgggttcg
    14941 aacggcatac ccatacggcg ttcgacttcc gcgcggaagt tgtccaagcc catattttga
    15001 atggtgtagc gggtgcgggc gtttttgcgg tcgctgcggt tgccgaagtc gcgctgcgtg
    15061 gttaccaccg cttcggcggc cttcagcgcg tgttccggag gcacgaaacc cagttccagt
    15121 gaaatgttcg gataggtttt ggtgttgccg tgttccatcg aaagcccgcc gcctgccaaa
    15181 acattgaagc cggcaagctg tccgttaccg tctgaaacgg cgacgaaatc caaatcgttg
    15241 ccgtagcagt ccacatcgtt caagggcggg atgacgactg cggttttgaa ttttcgcggc
    15301 agataggttt tgcccaaaat cggctcgtct tcttgaagga agtcgtcgga actttgaact
    15361 tttttgccgt ccacccacac atccagataa ccgcgcgtgc gcggcagcag gtgttcggaa
    15421 atctttttcg cgtattcgta agcctgccgg tgcagttcgg actcgatcgg gttggacgtg
    15481 caaagcacgt tgcggttcat atccgccgcc gtggcgatgg aatccaaacc cagtttgtgc
    15541 aagaggcggt gcatcgtctg caacttggct ttcggcacgc cgtgaaattg gaaggtttgc
    15601 cggttggtca gccggatgga gcggtaatga ctgttttccc gggcaaattt gtccagttct
    15661 atccattggg acggtttgat gatcccgccc ggcagccggc agcgcaaaag cataaatttc
    15721 aagggctcga gttttgcctc ggcgcgttcg gcgcggatgt cgcggtcgtc ctgctcatac
    15781 ataccgtgga agcggatgag ttggaagttg tcgcctttga agccgcccgt gagcgggtct
    15841 ttcaaatcgt ccaaaatcgt gccgcgtaaa aaattgcttt cggttttcag acgttcgttg
    15901 tcggatagcg gtttttcttg ccacgccaaa ccttttgtct tggtctgtac ggtcattttg
    15961 tgttcctccc gattatattt aatcaataaa catcacgctg atagcgtttt tcttcgcgca
    16021 gcatatccaa atattcttct gcgccctctt cgtccaaatg tcctgccccg ataatcacat
    16081 ccagcaaggc ggcttccacg tcttttgcca tttttgccgc atcgccgcac acatagatat
    16141 gcgcgccttc ctgcagccat tgccaaagtc cttccgcctg ttcgcggatt ttgtcctgca
    16201 catagatttt ttcttcctga tcgcgggacc aggcgaaatc gtacctgtgc aggaaaccgt
    16261 ctttggcaaa ctgctgccat tcggtttgat agagaaaatc acgggcaaaa tgcggattgc
    16321 cgaaaatcag ccagtttttg ccttccgcat tttctgcggc acgttgttgg acgaaggcgc
    16381 ggaacggtgc gacgccggtg cccgagccga tcatcacaat cggcttgcgg ctgtcttcgg
    16441 gcagcctgaa gccgtcgttg cgttccacaa acacgcgcac cgtgccgtcc tcttccagcc
    16501 ggtcggcaag gaaacccgat gcgccgcccg ccctggcgcg cccttcgtgt tcaaaacgca
    16561 ccgcgccgac ggtcaggtgc acttcgtccc ccacttccgc ctgcgacgag gaaatcgaat
    16621 acaggcgcgg cgcaagtggg cgcagcaggc cggcgaattg ttccgccgtc agttttgccg
    16681 ggaagcggtg cagcacatcg gcaatcggcg tgctttgcac aaagccttgc aaaacggcgt
    16741 tgtcggcagc aatacggtcg agttcgtcat catcggcgaa cggggcatag cctttgacaa
    16801 aggcgggggt gttttgcgtg agttcaaaat gggataacag tgcggaggca accggcaggg
    16861 tttttccgcc cgcctgtatt tccgttgccg gatcgatgcc gagcaggtct aggatttccc
    16921 tgaccagtgc cggatcgttg tcaaaccaaa cgccgagcgc gtcgcccggg aggtagtgca
    16981 aatccgaacc gctcaaatcg atttcgatgt ggcgcacgtc tttatcggat tggcgggcgg
    17041 tgattttctg attggccagc agggcggcgg gaaaggggtc tgccttgcag tacctgccct
    17101 ccgatgccgt ctgaaggccg gcggggggcg ttgtctgcgg cgcgggcgtt gcccggtttt
    17161 ttgcggcttc ttcttttaag agtgcggcga tattgtctgt ccaggcgttt gcggcggcgg
    17221 caaagtccaa atccgcatca acgcgttcga gcaggcgttt cgcccccaat tcttcaaaac
    17281 gtttgtcgaa atctttgccc gcccggcaga aattcggata ggaactgtcg cccaaaccca
    17341 gtacggcaaa ttggagtttg tccaatttcg gggctttttt gccgttcagc agtttgtgca
    17401 gcacgacggc ttcttccggc ggttcgcctt cgccttgggt ggacgtaacc agcagcaggc
    17461 ggcgttcgcc ggcgatgttt ttcgccttat agtctttcag ttcggcgcga ctgacttgga
    17521 tgccggcggc ttccaggctg tccgccgctt tgtcggcaac ggatttcgca ttgccggttt
    17581 gcgaggcgga aaggacggtt acggaaaaag gttctgccgt cggcaatgcc gtctgaagcg
    17641 cgggcagtcc tgcagatgcc ccgtttcctg cttttgccca agcgtagccg gacagccacg
    17701 cccattgtgc cgcgtccagc cccgacagga gctgcgtgat ttcgggcggc agaggcggta
    17761 atggcggatt tgtgttctgc atatcgtgtt cactcataaa atcatacctg ccgcaacagt
    17821 gccgtatgtc gcttcgtcta tcaggataaa cgaaccggcg gcggtgtttt ccgcataagg
    17881 cgttgccgta acgggttttt gaaggttgat gcggactttg gcgatgtcgt tcatcttcaa
    17941 ggattccgcg ccggcctctt gttccagcgt gcggacatcc aaaacgcttt caatttcccc
    18001 gacttttgcc ggcacggttt gcgtgccgtg cttgagcagg tatttgcgcg cggtgttgag
    18061 cggacgttcg tcaaaccagc aaagcgtggc ttccagatgt ttttgcgggg cgagcgggga
    18121 atttttatcg acaaaaaggt cgccgcgcga aacatcgatg tcgcggtcca gccggagggt
    18181 tgccgcctcg ccggcaaaag cctgcgccac ttcccctttc ggcgtgatga tttcggacac
    18241 ttcggcggtc agcccgttcg gttcgatgcg gacggtttgc ccgacggcga ccgaaccgcg
    18301 ttcgatgcgc ccctgatagc ctcggaaatc atcggccttg tcggcatctt ggcggacgac
    18361 cagttgcacg gggaaataaa aatcgtcggc ggtgcggctg acttcgtccg cccccggcag
    18421 ggtttccaaa atggacaata agggttcgcc tttataccaa ggcatattgc cgccggggta
    18481 aacaatgttg tcgcccaaga gtgcggacac cggtacgaaa tgcacgtctt tcaaaccgag
    18541 ctgttcggca agtcggcggt atgcctccac aatggcgttg aatttgtctt cgctgtaatc
    18601 cagcaggtcc attttgttga ccgccaccac aatatgcggg cagtcgagtt ggcggaggat
    18661 ggcggaatgg cgtttggtct gcggcagaag ctgcaagggc tgcgcgccga aatccagttg
    18721 ggatgcgtca accagcacga ctgccgccga agcggtgctt gcgcccgtaa ccatattgcg
    18781 cgtgtattgt tcgtgccccg gcgtgtcggc gatgatgaat ttccgtttcg ccgtggaaaa
    18841 atagcggtat gccacatcga tcgtaatgcc ctgttcgcgt tcggcttcca gtccgtcggt
    18901 caggatggag aagtctatgg tttctttcaa acctttgctt ttgccggatt ccaaggtttt
    18961 gatttggtcg gacagcaggg ctttgctgtc gtagagcagt cgtccgatta gggtgctttt
    19021 gccgtcatcg acgctgccgg cggtaatgaa gcggagcggg gtttggtgtt gtgccgtcat
    19081 attttcttcc tcatatctgc ttaaagggtt tttgaaattt agaaatagcc ttcttttttg
    19141 cgtttttcca ttgccgcctc gcttgcctga tcgtccagcc gggtcgcgct gcgttcggaa
    19201 atgtcggcaa ccgctgtttc tctgataatc tccgtcggcg tggacgcggt gctttctacc
    19261 gggcaggtgc agctgatgtc gccgacggtg cggaagcgga catcaaggat ttcggaggtt
    19321 tcagacggca ttttcggggt gagcggcgtt acagggacca gcagccccct gcgtctgacc
    19381 acttcgcgcc tgtggctgta ataaatcggc ggcagctcga ggttttcgcg ggcgatgtat
    19441 tgccagatgt cgagttccgt ccagttggaa atcgggaaga cgcgcatatt ttcgcctttg
    19501 tgcagcctgg tgttgtacag cgaccacagc tcggggcgtt gcgccttcgg atcccattgt
    19561 ccgaactcgt ctcggaacga gaaaatccgt tctttggcgc gggctttttc ttcgtcgcgc
    19621 cgcgcgccgc ccatcagcgc gtcaaagccg tttgcctcga tggtttccaa cagcgtaacc
    19681 gcctgcgccg cattgcgcga atcggtttct ttgcgtaaaa ctactgtgcc tttggcaatg
    19741 gagtcttcca cgcgccccac tatcaggcgg gcattgagtt ttgccgcctg cgcgtcgcgg
    19801 aaagcaatca cttcggggta gttgtgcccc gtgtcgatat gcaccagcgg gaagggcagt
    19861 tttaccggcc tgctgcccag ccggaaggct ttgcaggcga gggcgagcag gaccacggaa
    19921 tctttgccgc cggaaaagag cagggcgggg ttttcgcatt ccgccgccac ttcgcggatg
    19981 atgtggatgg attcggattc caaccagtcg agttgggcgt tgttcggttc ggttttcgtc
    20041 ataccatatt ccttatttct tctgtctgat atttatgaat tatttgtgca gcccgcattc
    20101 tttgctgttt ctgccttccc accaccaccg cccggcgcgg atgtcttcgc ccgccttgac
    20161 ggggcgggtg caggggtcgc agcctatgct gggaaatcct tgccggtaca aatcgttgta
    20221 aggcacattg ttggcgagga tgtatgccca cacgtcgtgt tccgaccagt cgaaaatcgg
    20281 gttgtatttg ccgatgcccc gtccggcatc gtattcggca aacggcagtt ccgtgcgtgt
    20341 ggcggattgt tcgcggcgtt gcccggtaag ccaggcgtcc gcgcctgcaa tggcgcggtt
    20401 gagcggttcg atttttcgga tgcggcagca ttcgcggcgc gcttcaacgc tgtcgtaaaa
    20461 ggcaaacctg cctttgcttt ccacataacg gtcggcatct tctcgaaccg gccggaaacg
    20521 ctttatccgc aaatggggat atgcgcgtcc gagcctgtcc agcaggttca gggtttccgt
    20581 gtggagcaga cccgtatcca aggtaaaaat gccgatattg aggttttcgc cggcgataag
    20641 gtcggtaatc accatatctt ctgccgcaag gctgctggca aaccgtgcat cccggtggct
    20701 gccggcaatc cggtgcaggc gttgtttgag ggtttccgtt ttttccgcaa gggcggtttc
    20761 gccgccggat ccgatatgcg gtatctgcca cagggcgggt ttgaacagtg tcgtttccat
    20821 ttttcccgcc ttatgccgcc cattgtccgg cattcagtcc gcccaatgcg ggatacgcct
    20881 gcccgactcg gttttctcct tcgccgtttt caccgaacca ggcgagtttt tcgtgcagcc
    20941 ccaccacttc gcctatgaca atcaatgccg gcttcggcgc gttttcggcg agttcggcaa
    21001 ggttggcgag cgtgccggtt gcggtttttt gagccggcag cgtgccttgg ctgataacgg
    21061 ctgccggcgt gtcgggcgag cgtccgtgct gttgcagccg ttcggcaatc agggcggctt
    21121 tgagcgcacc catataaatc accaaggtct ggcggctgcg ggcgagggtc tgccattcga
    21181 tgtcgggcgc atccgccttg cggtggccgg ttacgaaaac cgcactttgg gcataatcgc
    21241 ggtgcgtgag cgggatgccg gcataggcgg tcgcgccgac ggcggcagta atgccgggga
    21301 cgaccgaaaa cggaatctga tggcgtgcca aggtttccaa ttcttcgccg ccgcgtccga
    21361 acacgaaagg gtctccgcct ttcaaacgca ccacgcgcct gccttcgcgg gccagcctga
    21421 ccataagcgc attggtgtcc tcttgcgggg tgcggtcgcc ccgggcgcgc ttgccgacaa
    21481 aaatccgttc cgcatcgcgg cggacgaggg acagtatgcc gtctgaaacc agcgcgtcgt
    21541 aaagcaccac gtctgcctgc tggatttcct gtagcccttt gagcgtcagc agccccgcat
    21601 cgccgggacc cgcaccgacc aaagacacaa aaccgccttg ataattttgt tccaactgct
    21661 ttgccaactc ctgttcggct tgggcggttt gttgattttt aacgagggcg gcaaaacggc
    21721 cgttgaactg cttttcccaa aaacggcggc gttcggtaac ggatttcagt ttggctttga
    21781 ccgcatcgcg ccatcttccg gagatttccg ccatatcgcc caaagaatga ggcagcaggg
    21841 cttccagttt ttcacgcagc aaacgggcga ggacgggcgc gctgcccgaa ctggaaacgg
    21901 caatttgaat cggattgcga ttgataatcg aaggaaaaat aaaactgcaa taatcgcggt
    21961 cgtccaccac gttgaccggt ttttggcagc tttcggcaag ctggaaaacg cgccggttga
    22021 gggcttggtc gctgcttgcc gcaatgatga ggaaaactgt gcggatgtgt tcggcacgaa
    22081 attcttcggc aagccacagg attttgtttt ctgccgccag cgcacttaat tcagcattca
    22141 actgatttgc aacaaccctg acttgcgcgc cggcatccag caacaggctg attttgcgcg
    22201 cggcgaccga accgccgcct acgaccaata cggggcggcc ggcgaggttg gcgaaaatgg
    22261 ggaaataatt cactggcgaa ctcctttgct gtttgcccgc accttgtttc cgatacggtg
    22321 cgtcgcggca tttttgtcgg aatgcgggtc attttagaca aaaggatttt cccggttaaa
    22381 taataaaaag gcatttgtta gaagctgaaa gctatatgag ggcggctgcg gatgcggcgg
    22441 ttt
//
LOCUS       DAWFBO010000014        22337 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFBO010000014 DAWFBO010000000
VERSION     DAWFBO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.014"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(120..1034)
                     /locus_tag="WGO87_000384"
     CDS             complement(120..1034)
                     /locus_tag="WGO87_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0391797.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGO87_000385"
     CDS             1290..2759
                     /locus_tag="WGO87_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0391798.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3009..3380
                     /locus_tag="WGO87_000386"
     CDS             3009..3380
                     /locus_tag="WGO87_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391799.1"
                     /translation="MNKTLSILPAVILLGGCAVGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELDRRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGSDVMQMLNPSTR
                     NQALAALTVKTVSACFKRLYR"
     gene            3453..4862
                     /locus_tag="WGO87_000387"
     CDS             3453..4862
                     /locus_tag="WGO87_000387"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0391800.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGREALD
                     KWRGLAYPELAFEIMRLFVTDIPEGDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSEHTYVTSSPSMDISKASNFERFVFDLMDRNPL
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4908..5702
                     /locus_tag="WGO87_000388"
     CDS             4908..5702
                     /locus_tag="WGO87_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391801.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLAGIPLDAQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5890..6666
                     /locus_tag="WGO87_000389"
     CDS             5890..6666
                     /locus_tag="WGO87_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0391802.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6742..6848
                     /locus_tag="WGO87_000390"
                     /pseudo
     CDS             6742..6848
                     /locus_tag="WGO87_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7437..9908)
                     /locus_tag="WGO87_000391"
     CDS             complement(7437..9908)
                     /locus_tag="WGO87_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0391803.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMERIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9905..11083)
                     /gene="hflX"
                     /locus_tag="WGO87_000392"
     CDS             complement(9905..11083)
                     /gene="hflX"
                     /locus_tag="WGO87_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0391804.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11088..12368)
                     /locus_tag="WGO87_000393"
     CDS             complement(11088..12368)
                     /locus_tag="WGO87_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0391805.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12521..13246)
                     /locus_tag="WGO87_000394"
     CDS             complement(12521..13246)
                     /locus_tag="WGO87_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0391806.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGRRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13319..13996)
                     /gene="radC"
                     /locus_tag="WGO87_000395"
     CDS             complement(13319..13996)
                     /gene="radC"
                     /locus_tag="WGO87_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0391807.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14119..15468)
                     /gene="gshA"
                     /locus_tag="WGO87_000396"
     CDS             complement(14119..15468)
                     /gene="gshA"
                     /locus_tag="WGO87_000396"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0391808.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15728..17137
                     /gene="leuC"
                     /locus_tag="WGO87_000397"
     CDS             15728..17137
                     /gene="leuC"
                     /locus_tag="WGO87_000397"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0391809.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17234..17488
                     /locus_tag="WGO87_000398"
     CDS             17234..17488
                     /locus_tag="WGO87_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391810.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17550..18191
                     /gene="leuD"
                     /locus_tag="WGO87_000399"
     CDS             17550..18191
                     /gene="leuD"
                     /locus_tag="WGO87_000399"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0391811.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18360..19631
                     /locus_tag="WGO87_000400"
     CDS             18360..19631
                     /locus_tag="WGO87_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0391812.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19647..20378
                     /locus_tag="WGO87_000401"
     CDS             19647..20378
                     /locus_tag="WGO87_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0391813.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20412..21482
                     /gene="leuB"
                     /locus_tag="WGO87_000402"
     CDS             20412..21482
                     /gene="leuB"
                     /locus_tag="WGO87_000402"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0391814.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21645..22208
                     /locus_tag="WGO87_000403"
     CDS             21645..22208
                     /locus_tag="WGO87_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0391815.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSATIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5393 a         5867 c         5813 g         5264 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggtggcg ggatttttta ttttcccgat tgaagtataa tgtcgccgga ttcaaccgga
     2881 tattcaaacg gtttactcca acactcggaa cggcgcataa aacgccgccc tgcgcgttat
     2941 cccgaacggg gcggctaatc agatcctatc gccataaacg gcggggggtc aaccgaaaag
     3001 gaattgagat gaataaaacc ttgtctattt tgccggcggt aatcttactc ggcggctgcg
     3061 ccgtcggcgg cggtaacaca ttcggcagct tggacggcgg cacgggtatg ggcggcagta
     3121 tcgtcaaaat ggcggtcgag agccaatgcc gcgcggaatt ggacaggcgc agcgaatggc
     3181 gtttgaccgc gctggcgatg agtgccgaaa aacaggcgga atgggaaaac aagatttgcg
     3241 cttgcgtcgc ccaagaagcc cccgaacgga tgaccggcag cgatgtgatg cagatgctga
     3301 atccgtccac gcgcaatcag gcacttgccg ccctgaccgt caaaacggtt tccgcctgct
     3361 tcaaacgcct ataccgctaa ccttcttcag acggcatgaa gcaaatgccg tctgaatccg
     3421 ttttcccaca atttcgacag acgagaaaaa tcatgaaata tatcagcacg cgcggcgaaa
     3481 ccgcacacaa accgttcagc gaagttttat tgatggggct tgcacccgac ggcggcctga
     3541 tgctgccgga acattatccg caaatcgggc gggaagcctt ggacaaatgg cgcggtttgg
     3601 cttatcccga attggcgttt gaaattatgc gcctgttcgt taccgacatt ccggagggcg
     3661 atttgcgcga cattctgaac cgtacttaca cggaagcggc gttcggtact aaggaaatca
     3721 cccccgtccg cacgctttca gacggcatca aaatccaagc cttgtccaac ggcccgacgc
     3781 tggcgttcaa agatatggcg atgcagtttt tgggcaatgc gtttgaatat gttttaaaca
     3841 aagaaggtaa aaaactcaat atcttgggcg caaccagcgg cgatacgggt tcggctgcgg
     3901 aatatgcctt gcgcggcaaa aaaggcgtga acgtatttat gttgtcgccc gacggtaaaa
     3961 tgagcgcgtt ccaacgcgcg cagatgtaca gcttgcaaga cgagaatatc cacaatatcg
     4021 ccgtgaaggg gatgtttgac gactgtcagg acattgtgaa ggcggtgcag aatgatgccg
     4081 cgttcaagga aaaataccat atcggtacgg tcaactcgat caactgggga cgcatcgtcg
     4141 cgcaagtggt ttattacttt gcaggctatt tcaaagcgac gcaaagcaat gacgagcaag
     4201 tcagcttctg cgtgccgagc ggcaacttcg gcaacgtttg cgcgggacac atcgccaaac
     4261 agatgggttt gcctatccgc cgcctgattg tcgcgaccaa tgaaaacgat gtgttggacg
     4321 agtttttcaa aaccggcgca taccgcccgc gcaacagcga gcatacttat gttacctcca
     4381 gcccgtcgat ggacatttcc aaagcgtcca acttcgagcg tttcgtgttt gacctgatgg
     4441 atcgtaatcc tctggaaatc aatacgctgt gggcggaagt ggcggcggga aaaggctttg
     4501 acctgcggtt tgctttagaa aaagtcggtg gcaaatacgg ttttacctcc ggcaaatcca
     4561 cccaccccga ccgcctcgcc accatcagac aggtttatga gcaagaccaa gaactcatcg
     4621 acccgcacac tgccgacggc gtaaaagtcg cccgcgaagt gcgcgaagaa ggggaaacgg
     4681 ttgtgtgttt ggaaaccgcg ttggcagcga aattcgatgc gaccatacgc gaagccgtcg
     4741 gcgatgccgc cattccgcgc cctgccgcgc tggaaggttt ggaaaacctg ccgcagcgcg
     4801 tgcaaaccgt gccgaacagt gcggatgcgg taaaaggcat catcgaacaa acccttgcct
     4861 gatattccgt acaggggcat tccgtccgaa cttaagattg aaagaaaatg accttaccta
     4921 tgcaggaaac ccgtttttct atactgctgg acgaactggc ggcaaaacag gaggcagcca
     4981 tcgctcccca cctgcttgcc gacggaacca aggtatgggt acgcaaagca ggcaggcaca
     5041 atgcgcggtg gcgttacgcg ctgctcggta tggttgcccg atatttgaaa ctgggggtgt
     5101 tgaagccggt tcccagcctc ggcggcgagc ctgccattgc aaccgaatca aaacgcctgt
     5161 acgaattgcg ctcggcgggg atagccgttc ccgaattgct cgccgtcagg aaaaatgccc
     5221 ttatgttcgg caatttggca ggcatcccgc tcgatgcgca aatccgccaa gaagccgaag
     5281 ccggaaaggc ggacgcttgg cttgccggtt tggaagccat tgcacgcgtg cataaaaaac
     5341 agcagttcct cagtcaggcg tttgcacgaa atatgatgtc ggacggaaaa aacatcagct
     5401 ttttggattt tgaagacgac ccttccgagg tcctgaccat agcgcaatgc caagcccgcg
     5461 attggctctg ctacattcat tcgaccgcgc tgatattgaa aaacggcgga ctgctggaag
     5521 cggctgcgga aaaatggggc ggcgtattgt cagatcagcc tgccgaaata caaaagctga
     5581 ttgccgccac cgtcaaaccg atcctcccga tacgcaggct ggaacacccc cgctggggac
     5641 gggacgcgct caggctggca gcctcgattt ccctgatttc ccttgccgat atgccgccgt
     5701 aagccgccgc ccgccccacg atgccgtctg aagcccttca gacggcatat aaccaacgcg
     5761 catcaagcat tgtcgcaacc atccgtttcg tttagaattt tggctttacc ccgaatcggg
     5821 cagacatttt cactctgccg cacatcccga acaagcacta tccatttctt ttactgacag
     5881 gaaacccaaa tggcagcatt caatacccaa aaagtattgt ccgtacacca ctggacagac
     5941 gcatatttta cctttacctg cacccgcgac gaatcgttgc gctttgaaaa cggacagttc
     6001 gttatggtcg gattgatggt ggacggcaag ccgctgatgc gcgcatacag cgtcgcctcc
     6061 gccaactggg aagaacacct cgaatttttc agcattaaag tccaagacgg tccgctgacc
     6121 agccgcctgc aacacctcaa agtcggcgac gacgtgttaa tcagcaaaaa accgaccgga
     6181 accctggttg ccggcgacct gaatccgggc aaacaccttt acttgttgag caccggtacc
     6241 ggcatcgctc ctttcttgag catcaccaaa gaccccgaaa tttacgagca atttgaaaaa
     6301 atcatcctcg tacacggcgt gcgctacaaa aaagatttgg cgtactacga ccgctttacc
     6361 aaagaattgc ccgaacacga atacctcggt gacttggtta aagaaaaact gatttactac
     6421 ccgattgttt cgcgcgaaga attcgaacac cacggtcgcc taaccgacct gatggtgagc
     6481 ggcaaactgt ttgaagacat cggcctgccc aaaatcaacc cgcaagacga ccgcgccatg
     6541 ctgtgcggca gccccgcgat gctgaaagat acctgcaaag ttttggacga tttcggactg
     6601 accgtctctc cgaaaacagg cgtacgcggc gattacctga ttgagcgtgc gtttgtagat
     6661 caataagctg tttgaaaatc ccgtctgaac ggcaccagca atcaaaaacc ccgaatcctg
     6721 ccaatcagga ttcggggttt ttatagtgaa ttaaatttaa atcaggacaa ggcggcgagc
     6781 cgcagacagt acaaatagta cggcaaggcg agccaacact gtaccggttt aaatttaatt
     6841 cactatatag tgggtaaaaa ttagaaatgc acatttccgt cattccaaca caggtgggaa
     6901 tccggactta cctgtttcat tatcgtttga aaataaaaga gaaaccaaac atatagattc
     6961 ccgcttgcgc gggaatgacg acggtgtttt gccgtagcca aagcagaaaa acatccctat
     7021 tgtcatttca tcatgatatg acaataggat gttaaaaatt aattatcatt atatgattga
     7081 tatataagaa aaactgtccc aataagaaat gttatgcaga aaataaaatg aaaaggatgg
     7141 ttttcattga aaatggttgt tctgccaccg tcatacacag agccagtatt tggaaaaaaa
     7201 tgatccatat tgacatcatt attccatttg tcgcataaat ccacttttta attttttcca
     7261 cattaatata atctcttatt taataaaatc aaaataaaaa tcaaatgcct aacaattcga
     7321 tctcaaattt tgaacgccaa cgtgtttgag cggtaacaat acatacttta agtttgcaat
     7381 tcttcatcgt cttatttttg ttttcatcaa ctatacgtct aaagcaacgc cgtccgtcat
     7441 tgttcggacg gcattttttc agactgcatt ttcccccgtt tgtgaaggcg cagggcgttg
     7501 cccaaaaccg ccagcgaact gaagctcata cccagtgcgg ctatccacgg ttggacatag
     7561 cccaaaacgg caagcggtac ggcaatgata ttgtacgcgc ccgcccatat caggttttgc
     7621 cggataatat ggcgggtgcg ccgcgcctga tcgagcaggt gggcgacggt acgcaaatct
     7681 tcgttcaata acacaatgtc cgcgccgtcc ctcgcaatat ccgtcccgcc cgctgcggcg
     7741 gcggatacgt ctgcctgcgc caaaacgggc gcgtcgttga tgccgtcgcc tatcatcagc
     7801 acttttttcc cttctttttg caaggctttg acgtattcca gtttgtcctc gggcatggct
     7861 tgggcgcggt agtgcgcgac acccagggcg cgtgcggttt cggcaacggc ggtttcgcgg
     7921 tcgccgctga ggatgtgcag ggtcaggttt ttgcctgcca actgccgcac cgcctccgcc
     7981 gcgctgtctt tcaaggggtc ggtcaggtag aacacggctt ggaaaccgct ttgactgccg
     8041 aggtaaaccg cgctgccgcc gccttctgtt tgcggttctt tacctgaaat ttcggcgaca
     8101 taggatgccc tgcccaatgc ccaaacctgt gtttcgccgt tgacggtcag ttgcgcgccc
     8161 acgccttcgc cgatgcggtt gaggcgttgt ttaatagcga tgtcggggac gctgccgtct
     8221 gaaatgcggc agttgaggat ggcgcgggca aggggatgtt cggactgttg ttctaaagcc
     8281 tgcgccaccg cgagaacaaa ggcttcgtct gtgcctctca acaatgagat acggcggacg
     8341 gcgggtttgc cttgggtcag cgtgccggtt ttgtcgaaga tgatgtcggt ggtttgggcg
     8401 agggtttcga ttgcctgctt tccgccgatt aaaatacctt cgcgcgccag cgtaccggta
     8461 gaagctgcca gcgcggtcgg cgtggcaagc gataaggcgc aggggcaggt aatgaccagc
     8521 agggcgacgg taatccacaa tgcggtgtgc gcgtcggcgt acagcgtcca gccgatgaag
     8581 acggggacgg caagcaggag ttcgccgaat atgaaagacg aggcgtattg ttccgccaac
     8641 tcggcagtgc gcggtttttg cgctaaggcg cggtcgagca ggcggacgat gtgcgacagt
     8701 cgcgtgccac cgccggtgcg gtcggtgcgt ataatcaggg ggctttgcgt gttgagtgtg
     8761 ccggcggtta ctttttcaga cggcattttg gcgacgggca ggctctcgcc ggtcagcata
     8821 gattcgttga cggcactgct tccttccagc accgtgccgt caacggggat ggtttcgccc
     8881 ggtttgacca gcacgatatc gcccgccttc aatttgacga cagctgcctc gcaggtttcc
     8941 tgcgtatcgg ggtaatcggg catatgatgg caaaacgcag gaatcagctt caccagcctc
     9001 tcggcggcat cgcctgcctt acggcgggca atgcgttcca taaagcgtcc gcccagcagg
     9061 aaaaacagca gcatcgcgat ggattcgaaa tacatcccct gccccgcatt tgtcgcaagg
     9121 ctgtaaacgc cggcgataaa ggtcatgatg atggcgacgg taatcggcgt atccatgccg
     9181 acgcggcggt ttttcaagtc gcgcagcgcg ccttgataaa acgggacggc gcaatagaat
     9241 acgacgggca gcaccattaa aaagccgccc caatggagga tttgcaggaa atcgggttcg
     9301 atgtcgccgc cgtaaaggta ggtcggcagc gcgaacatca tcgtctgcat catccccagc
     9361 ccggcaacgg cgaggcggac gatgtattgt ttgcgttctt tttggttggc ggcttcgatt
     9421 ttttgcgcgt catagggtgc ggcggtgtag cctatctgcc tgattttcaa cagaatgtcg
     9481 gaaaggcgga ttttgccgtc gtcccagacg acgcggcagc ggtgcgtgct gtaattgagg
     9541 tcgatgcgga cgatgccgtc tgtacgcaaa agctgctgtt cgatcagcca gacgcaggcg
     9601 gcgcaggtga tgccgccgag cattaaaacc gcctcgcgcg tgccgccgtg ggtttccaca
     9661 aagtcggact ggacttcggg caggtcgtac aggcggattt ggtcgaggat ttcttggggc
     9721 ggcagctcgg ttttttgcgc gtcggcggtg cgttgtttgt aataactgcc caagcccgcg
     9781 tcaataatgc tttgtgcgac cgcctgacag ccggcgcagc aggtttcgcg gtcttcgttt
     9841 tcgtaacgga cagtcaggtg gaggtgttcg ggaacatcca gaccgcagtg gaaacaggtt
     9901 tttttcatgg catttcggtt tcgtctgtgt ttggtgcggc ggcacaagac tcggcaatgg
     9961 cttcgcgcag tgcgtctata ccggtatttt cagcaacgga aatgcggacg gcggcaattt
    10021 ttcccgcagc gtcgcgccat atgcccgtgt tttgttcttc agacggcagc aggtcggttt
    10081 tgttgtacac cttgatgcac ggaatatcgc cggcatggat ttcccgcagt acgttttcca
    10141 cgtcttcaat ctgctgtccg ctgttcgggg cggcggcatc gacgacgtgc agcagcacat
    10201 cggcttgcgc ggtttcttcc agcgtggcgg aaaatgccga aatcagtttg tgcggcaaat
    10261 cactgacgaa tccgacggta tcggtcagga taatgctgca ttcgggactg atgtacagcc
    10321 gccgcgccgt ggtgtcgagt gtggcgaaaa gctggtcttt cgcatatatg cccgacttgg
    10381 tcagccggtt gaacaggctg gatttgccga cattggtata gccgaccagc gcaaacgttt
    10441 tgattgtgcc cgattcgcgg ggcttgcggc gcagggcgcg ctgttttttg aggttgacaa
    10501 gctgtttttt caaggcattg atccgatggg cgatcaatcg gcggtcggtt tccagtttgg
    10561 tttcgccggg gcctttcatg ccgataccgc cgcgctggct ctgcagatgg ccgtaaccgc
    10621 gtatcaagcg tcccgccaaa tggctcaatt gcgccaactc gacttgcagc ctgccttcct
    10681 gcgtgcgggc gcggcgggcg aaaatcgcca gaatcagccc taccctgtcc aatacgcggc
    10741 atttcagttc tttttcaagg ttgcgttcct gcgtgggcgt gagttcgtgg ttgaatacga
    10801 ccaaatcgat gccgtctgcg gcaactgctt ctgacagctc cgccgccttg cccgtgccga
    10861 caaacagcgc ggtgtgcgga cggtcgcgtt tggcagtctc cacgcgcacg gaatcgccgc
    10921 ccgccgcttt gaccagctcg acagcttccg ccaatgccgt ctgaaaaccg ttcagacggg
    10981 tggcactgct gcccgtgcca tctttgtcca acattacgcc caccagcatg acgcgttcgg
    11041 gttgcgcttg ggtactgccg ttgcggcctg ttctgcctgt catgtattca atcctgtaaa
    11101 taaagggtta cgcctcgttc cgtttctcct tccggtgcaa gcgtgatgcg gctgcccgcc
    11161 attccgtcaa cgctgaagac gatgccgccc gcctcgtgcc ttcctatgga caggcggctg
    11221 ccgggtcgct gccggtaggt ataacgggta cacgcttcca aacaccagcg tccgcgccag
    11281 tggatgaaat atttttcgcc ttttctaacc gcaagtgtct cgccgccgtt tacctcaaaa
    11341 taagggtaat cgggttttcg ggttctaaac tgccatttcg cctgcgcgcg ccgtccgttg
    11401 cggacatagt cgaataccgc cgtataaagc gtaccgtatt ccaaaggctt gagcgggaaa
    11461 agcgcgaatt ggtacgcggt cagcctgccg ttggggtcgt tgccggcggt taaaaccctg
    11521 acggggcgga tttcgttttt accctgatac agcttgaaac ttttcatcgt aattttgcct
    11581 gccgcctcgg aaaaatcaat gctggcagga ttgcccgtga tttcatattc cggcacgggg
    11641 tctggacgct cgccgtggaa ataaggcagt gcgccgttgc cgacgggata ggctgtatag
    11701 agcagctcct gtgcgggcat ggcttcgtcg gtgtacacga ccgcaccgtt atggcaggcg
    11761 ttgcggtaat atttccgtcc tgcttccggc tgatttctgc cttgggcgca atgccgctca
    11821 aacctgccgt tgccctgatt gaatacgaga acggttttac cgttttcgcg cacaaatgcc
    11881 gctcctgcct catccgtatg gcggtcaagt agggaaaggc ggtggtagat tgcgctcatt
    11941 aatccgtcca cttggcgttg ctgcgtgcgg atgtcgctgt cggacgattc ggcggcttcc
    12001 tcttccgtgc tgatgttttc atgcacgccg ttgtagagat acccggcaag gcgtgtgcgt
    12061 tcggtcagct tttgtgcggt gtagtgcgga ttgtcgggat ggtgttcgcc gtgtccgtct
    12121 tcgggattga gcgtgagata gcgtgcgtgc ctgcgggcgg aattttccaa aaccggcgcg
    12181 tgtgccagct tgtgcaaacc gatttgggct cggatgcggt tgaggtagtg cagcgcatcg
    12241 ccgccgtccc tgatttgcgg tgcggacgga tagacaagtt cgcccgcggg cagggattgg
    12301 ttttgggtat ggtagaaaac gcctgccgcc gaacccaaca ggagcagccg aataaaaagg
    12361 gatttcataa tgccgtcctc ccgacgcggc aacggggtgt tttatcaaaa cggcaaactt
    12421 gccaatgcgg tttccgattg cagattgcca aaccggcaaa cctttccccg tgccgatgcc
    12481 gaacccgatg ccgtctgaac cttcagacgg catcgggtgt ttatttgtcc acccggatgg
    12541 gggcaggttc gtattgtgtc gattcgtcgc cgtaatacag cacgccgagt ttgatgggaa
    12601 tgcgtccctg cgatttgcgg tgggcattgg aatcgcgcaa ggaataggcg caaccgcaat
    12661 attcctgctg gtagaagttt tcacgtttgc tgatttcaat catgcgcgcg ctgccgccgc
    12721 ctttgcgcca gttgaaatcc caatacacca catcatcgta aggcgcggcg gcacggcggc
    12781 cgcagtcgtt gatttgcgcc atatttttcc agcgtgaaat gcccagcgaa ctggtaaaga
    12841 cggggaaccc gtgttcatgc gcgtattgcg ccgccttttc aaaacgcata tcgaaacaca
    12901 tggtgcagcg gatgccgcgt tccggctcaa actccatgcc tttggctttg gcaaaccatt
    12961 ctttgcggtc gttttcgtag tcgtcgtctt tatcgatgaa aggaatgccg aacttttccg
    13021 caaagcgcac gttttcctct tttcggagca tatactcttt gtgcggatgg atattgggat
    13081 tgtaaaaata aatggtgtag tcgatgccgg aggcaagcat cgcctccatc acttcgccgc
    13141 tgcacggggc gcagcaggaa tgcagcaaga cttttttatg tccgccgggc gggacgagta
    13201 tggggcggtc aatgtcggta acggtaggtt tgttttgtgt ttccattaac ttaacaatat
    13261 ctgtcgaata cggttgaagc ggctatttta tcagagccgc cgcatgtaaa acagggggtc
    13321 agggcatcag cccgagctgc ctgaacgaac ggacggtttg cgccgttacg ataaaatggt
    13381 cgagcagcga cacatcgacc agcgacattg cctgtgccag ccgccttgtg aacatgatgt
    13441 cttcctgcga aggttcgggc gagccgcccg gatggttgtg cgcaataatc aggctgtcgg
    13501 catattcgtc caatgccagt ttgacgattt cgcggatgta aatcgtgttt tccgcaaccg
    13561 taccgcgcga cagctctctg accgcaatca gttggttttg gcggttcagc agcagcgcga
    13621 cgctgacttc gactttttcc tgccccaaat gaaagcgtaa ataatcggcc accgtatccg
    13681 gatcggacag gacgatgctc tcctgcaatt cttccgccaa tatccgccgc ccgatttccc
    13741 tgaccacggc aaactgtgtg aaacttgccg tccccatccc tttgtatgcc gacagttttc
    13801 cgacctccgc gctcatcagc ctccccaaac tgccgaactc ctgcagcaaa taacgcgcca
    13861 aatcgaccgc actcattccg cgcgtgccga cgcgcagcag gattgccaaa agttcggcat
    13921 cactcaacgc cgccgccccg cgttccaaca gcttttccct gggtctttcg ccttccggcc
    13981 attgcttgat gctcatgttt tccctttttt aaatagtctg agatgccgtc tgaaacagat
    14041 gttcccacac gccaaatatc cgaaatgccg tctgaaccgg atttccgctt cagacggcat
    14101 ctgtatttca aaacagtctt acgccgtttc ttccaactcg attgaagccg ccagaagcga
    14161 caggcgtgcc attacgccgt aaacgtagag gcggttgtgt tcgttgtcca cgtcgcagtc
    14221 tttttcagag cgcggcatac ccagcgagtc ccattgttcg aatacgcgct tgcaggcttc
    14281 gggcgcttct tgggaattat cgccgttacc ggtaggaatg ctgttagaca gcggaacaaa
    14341 caccataccg ccggcgttga ggttttcgtc cgcaccgcgc ccttcgtgta cgcggaaaaa
    14401 gccgccgatg acgaagcggt ccatcatata cacgacgggt tcgcacaccg cgccgtttaa
    14461 ggtttcataa gtataaatcc cttcttggac aatcacttcg ctgacttcca agccttcttt
    14521 gactttcgcc attttattgc ggtttttacg gttcaatccg cgcacttcgt cggcggattt
    14581 gacgctcatc acgcccatgc cgtaagtgcc ggcatcggct ttgacgatga cgaaaggttt
    14641 gtcggtaatg cccgattcgt cgtatttggc ttgaattttc gccagcacac gttctaccgc
    14701 ttccgccaac gcgtcttcgc cttcacgccc ttggaagtcc aaaccgccga ttttttcaaa
    14761 ataagggtta atttgccatt cgtcgatgcc gattaacttg gcaaattcgg cggtaacttg
    14821 gttgtacgcg ccgaaatgat ttgttttgcg gcgcgtcgtc caaccgccgt gcaacggcgg
    14881 caaaacggtt tggctgatgc ctttgaggat gtcgggaatg ccggcagaca aatcgttgtt
    14941 caacaaaacc acgcaaggcg aaaagccgtc tgcaagatgg acgcggtcgc gggtacgcaa
    15001 taaaggttcc aacaggattt tgtcgcccaa tgcagtttca aattcggtcg gttcggttac
    15061 ttccggattc aggctgccca agcgcacttc ataccctgcc gaacgcaaaa tctcgccgag
    15121 ggcgtaaatg ttttgcaggt aaaacgtatt gcgcgtgtgg ttttcgggaa taatcaatac
    15181 ggatttcgcc gtttcgcagg cgcgttgcac cgcatcttgc gcggcaaccg ccgccagcgg
    15241 gataaagttg ggattcagat tattgaagcc gccggggaac aaattcatat cgatagacga
    15301 aattttgtaa ccggcattgc gtatatcgac cgaaccgtaa aacggcgggc ggtgtacgct
    15361 ccactgcgtg cggaaccacg cctcgatttt ggcgtgattg gacaggattt ttgcctcaaa
    15421 cgcctgaagt tgcgccgaat gttcgggcga catcaccggt aatttcatct ttcgtctgcc
    15481 tctgtgtgtt ttttgacaag atgcagatga taatgccttt gaaagaatga cgacaagtat
    15541 tctttcgtat cgaatcatgc tttagatata actgtttact gaaatggaca atacgtctaa
    15601 ttttttcgtt aaaattgttt gcaaaataac accggctcaa aaattagaca aaatttgttg
    15661 cgcggtataa agaatacgtc taaaatccgc ccaatccgca ttaccattta tccaaaagaa
    15721 caacatcatg acagcacaaa ccctctacga caaactttgg aacagccacg tcgtccgcga
    15781 agaagaagac ggcaccgtcc tgctctacat cgaccgccat ttggtgcacg aagttaccag
    15841 ccctcaggca tttgaaggct tgaaaatggc ggggcgcaag ctgtggcgca tcgacagcgt
    15901 cgtctccacc gccgaccaca acaccccgac cggcgattgg gacaaaggca tccaagaccc
    15961 gatttccaag ctgcaagtcg atactttgga caaaaacatt aaagagtttg gcgcactcgc
    16021 ctattttccg tttatggaca aaggtcaggg catcgtacac gttatgggcc ccgaacaagg
    16081 cgcgaccctg cccggtatga ccgtcgtctg cggcgactcg cacacttcca cccacggcgc
    16141 attcggcgca ctggcgcacg gcatcggcac ttccgaagtc gagcacacga tggcgaccca
    16201 gtgcattacc gcgaaaaaat ccaaatccat gctgattgcc gttgacggca aattaaaagc
    16261 gggcgttacc gccaaagacg tggcacttta catcatcggg caaatcggca cggcgggcgg
    16321 tacaggctac gccgtcgagt ttggcggcga agccatccgc agcctttcta tggaaggccg
    16381 catgacttta tgcaatatgg cgatcgaagc cggtgcgcgc tcaggcatgg ttgccgtcga
    16441 ccaaaccacc atcgactatg taaaagataa acccttcgca cccgaaggcg aagcgtggga
    16501 caaagccgtc gagtactggc gcacgctggt gtctgacgaa ggtgcggtat tcgacaaaga
    16561 ataccgtttc aacgccgaag acatcgaacc gcaagtcact tggggtacct cgcctgaaat
    16621 ggttttagac atcagcagca aagtgccgaa tcctgccgaa gaaaccgatc cggtcaaacg
    16681 cagcggtatg gaacgcgccc ttgaatacat gggcttggaa gccggtacgc cattaaacga
    16741 aatccccgtc gacatcgtat tcatcggctc ttgcaccaac agccgcatcg aagacttgcg
    16801 cgaagccgcc gccatcgcca aagaccgcaa aaaagccgcc aacgtacagc gcgtgttaat
    16861 cgtccccggc tccggtttgg ttaaagaaca agccgaaaaa gaaggcttgg acaaaatttt
    16921 cattgaagcc ggttttgaat ggcgcgaacc gggctgttca atgtgtctcg ccatgaacgc
    16981 cgaccgcctc gcaccggggc aacgctgcgc ctccacctcc aaccgcaatt ttgaaggccg
    17041 tcaaggcaac ggcggacgta cccacctcgt cagccctgcc atggcagccg cagccgcagt
    17101 gaccggtcgc tttaccgaca tccgcatgat ggcgtaagta ttttcaggcg gtctttaaga
    17161 atttgacgcg gccgtctgaa atatgtcccg taaggcagaa acacctttaa tattcaaacc
    17221 aaggagttta accatgaaca aacttttcat taccgccctg tccgccctcg ccttgtccgc
    17281 ctgcgccggc acttgggagg gcgcgaaaca agacaccgcc cgcaaccttg acaaaacaca
    17341 ggccgccgcc gaacgcgccg ccgaacaaac aggcaacgcc gtcgaaaaag gctgggacaa
    17401 aaccaaagaa gccgtcaaaa aaggcggcaa tgccgtcgga cgcggcattt cccatctcgg
    17461 cggaaaaatc gaaaacgcca ccgaataacc gattcgcgat gccgtctgaa accgcttcag
    17521 acggcatcgt cagcacaaga cgcaaaccca tgaaagcctt taccaaaatt accgccatcg
    17581 tcgccccgct cgaccgcagc aacgtcgata ccgacgccat catccccaaa caatttttaa
    17641 aatccatcaa acgcagcggc ttcggcccca atgcctttga cgaatggcgt tacctcgacc
    17701 acggcgaacc gggcatggac aacagcaaac gcccgttgaa ccctgatttc tcgctgaacc
    17761 agccgcgtta ccaaggcgcg caaatcctgt tgacgcgtaa aaacttcggt tgcggctctt
    17821 cacgcgaaca cgccccttgg gcattggacg actacggctt ccgcgccgtc atcgccccca
    17881 gcttcgccga catcttcttt aacaactgct acaaaaacgg ccttttacct atcgtgttga
    17941 ccgaagaaca agtcgatcgg cttttcaaag aagttgaagc caacgaaggc tatcagctct
    18001 ccatcgacct tgccgaacaa accctgacca caccaagcgg cgaaacattc acattcgaca
    18061 ttaccgaaca ccgcaaacac tgcctcttaa acggcttgga tgaaatcggt ctgaccctgc
    18121 aacacgccga cgaaattcac gccttcgaag aaaaacgccg ccaaagccag ccttggctgt
    18181 ttaacggtta agcctatgcc gtctgaagac cgttcagacg gcattcctga taacatataa
    18241 ttgctgcaac taatgtaaac agaggggcta taaattgctt tcatctttca agcgggctta
    18301 gcaacgctct aaattaatgc aatacagctc tatttcaaat aaatttttag atttttaata
    18361 tgcaacagat taaatttatt gacttatttt ccgggatgag cggtatcagg aaaggatttg
    18421 aacaagcctg tcggaaacag tcggttgctt gcgaatgtgt ttttacctca gaaatcaaac
    18481 cggcagcttt ggaagtattg aagcaaaact accctgatga agtgccgtat ggagatataa
    18541 cgaaaattga aacgggagat attcccgatt ttgacatctt gttggcaggc ttcccttgcc
    18601 aggctttttc tttcgccgga aaaagattgg gctttgaaga tacgcgggga acgcttttct
    18661 ttgatgtggc aaggatttta aaggcaaaaa aaccaaaagg ttttattttg gaaaatgtgg
    18721 aaggattggt gacgcacgat agaaaagatc cgacacaaaa aataggacgc accctgaccg
    18781 ttattttgga aaccttggaa acattgggct actatgtttc ttggaaagtt ttaaatgcaa
    18841 aagatttcgg cattccccaa aacaggaagc gcatttatct gacaggcagt ttgaaatcca
    18901 aacccgactt gtcttttgaa acaaccctaa gtccgaaatt aaaaaatatt ttggaatcgg
    18961 gactgcctac cgaaagcagt cctttcatca aaaaattgct aaagaaattt cccccgtccg
    19021 aactgtacgg aaaatcagta aaggacaaac ggggagggaa aaacaatatc cacagttggg
    19081 atattgaatt aaaaggcaca gtaaccgaag aagagaagca attattaaat atgcttctaa
    19141 aagaacggag gaagaaaaaa tgggcttcag aaatcggcat cgattggatg gatgggatgc
    19201 ctttgacaaa agcgcaaatt tcaactttct ataaacaccc caatcttcaa aatattttgg
    19261 acagcctgac agataaaggc tatttggttt tggagcatcc gaaacaaaaa attggcgggc
    19321 gaagaatcaa agatgaatcc ctgcccaaag gctacaatat tgtttcagga aaaaaatcat
    19381 ttgaaatcaa taaaatatta gacccaaatg atgttgcgcc aaccttagtt gcaatggata
    19441 tggagcacct tttcgtcgtt gacaacggcg gtttgcgcac acttaccgga aaagaaggtt
    19501 tacgcttatt cggctatccg gacgattatt cgtttgatat tcctaaaaaa gacaaatacg
    19561 atttattggg caataccgtt gccgttcctg tgattaaggc ggtatctgaa aggcttctgc
    19621 atactttata aaccgatagg gataaaatgg taaaacttac tgcacaacaa atatttgata
    19681 agttgctgaa tgaggaaaaa atcttatcgg tcaatggtca aattagattt ttcttgggcg
    19741 atgtggatat tatcgtcaaa caaaaagatg tcgtcggcaa catcattcaa gaatggctcg
    19801 gcggatggtt gagaaaaagg gaaattgaat ttgatgtttc aaccaatacc caaatgcccc
    19861 ccgatttctt tttaaataaa aaagaccgca gtagagaatt gttggaagta aaggcgttca
    19921 accgaaatgc cggcccgggt tttgatattg cagattttaa aatgtattct gatgaaatca
    19981 ttcataaacc ctatatgctg gatgtagatt atttaatatt cggttacgat atggacgaca
    20041 acggcaatgt aaccatcaag gatttatggc ttaaaaaagt atggcaaatt acgagaagca
    20101 tggatgggtg ggcaattaat cttcaagtca aaaaaggcgt ggtgcataaa atccgcccgg
    20161 gtgtttggta cagcataaat aaaaagaata tgcctatgtt tgaatgcttg gaagattttg
    20221 tttccgcaat tgaagaaacc gtttatcaaa acccggcaac acggcataac gcatccttat
    20281 ggaaaaagaa atttgaggaa gcctataaaa aacactacaa ccgatcgatt tccatacctc
    20341 gttggcacga aattgcacac aaatacaaaa agaaataacc cttcaaccat caacccataa
    20401 ggaaacttga aatgaccaaa catatcgcca tcctccgagg tgacggcatc ggtcccgaaa
    20461 tcgtcgccga aaccgtccgc gtactcgaca aactcatcgc ccaaggcttg gacatcagct
    20521 acgaatacgc cccattgggt ggtgaagcct acgacgaata cggccatcct tatcccgaat
    20581 ttacgcaaaa cctctgccgc aaagccgatg ccgtcctgct tggtgcagtc ggttctcctc
    20641 aatacgacaa cctcgaccgt ccgttgcgcc ctgagcgcgg cctgttggca atccgtaagg
    20701 atttgaacct gtttgccaat ttgcgtcctg ctgttttata tcccgaatta gccaacgcct
    20761 ccacgctgaa gcctgaaatc gttgccggtt tggatatttt gattgtgcgc gaactcaccg
    20821 gcgacatcta tttcggcgaa ccgcgcggca tccgcgttct ggaaaacggc gaacgcgaag
    20881 gctacaacac catgaaatac agcgaaagcg aaatccgccg tattgcccat gttgccttcc
    20941 aatccgccca aaaacgcagc aaaaaagtct gttccgtagg caaagccaac gttttggaaa
    21001 ccaccgaact gtggcgcgaa atctttgaag aaatcggcaa acaatatccc gatgtcgaac
    21061 tctcccatat gtatgtggac aacgccgcta tgcagctcgt gcgcgcgccc aaacaatttg
    21121 acgtgattgc caccggcaac atcttcggcg atattttatc ggacgaggcc tctatgctga
    21181 ccggctccat cggtatgctg ccttctgctt ctttggacga aaacggcaaa ggcctgtacg
    21241 aaccgtctca cggctccgct cccgacatcg ccggacaaaa caaagccaac ccgctggcca
    21301 ccatcctctc gctcgcaatg ttgctgcgtt acagcctgaa cgacgaagcg cgcgcgcaac
    21361 aagttgaaaa cgccgtccaa aaagtgctgc aacaaggctt gcgtaccagc gacatttacg
    21421 aagaaggcac aaaactcgtt tcctgctccg aaatgggcga tgcgatactc gccgccttgt
    21481 aaaaaaacaa agcgcggatc acaccgcgct ttttcatctg cccttttccg cccgcaccgt
    21541 tcgtcaaccg cctgcaaacc caccgtttac cggcaggaaa tgccataaat ccccgcctat
    21601 gttaggataa acacctttat cggcccacac aaaggattat catcatgaaa aaaatcatct
    21661 tcgccgcact cgcagcggca gccgttggca ctgcctccgc cgccacctac aaagtggacg
    21721 aatatcacgc caacgcccgt ttcgccatcg accatttcaa caccagcacc aacgtcggcg
    21781 gtttttacgg tctgaccggt tccgtcgagt tcgaccaagc aaaacgcgac ggtaaaatcg
    21841 acatcaccat ccccgttgcc aacctgcaaa gcggttcgca acactttacc gaccacctga
    21901 aatcagccga catcttcgat gccgcccaat atccggacat ccgctttgtt tccaccaaat
    21961 tcaacttcaa cggcaaaaaa ctggtttccg ttgacggcaa cctgaccatg cacggcaaaa
    22021 ccgcccccgt caaactcaaa gccgaaaaat tcaactgcta ccaaagcccg atggcgaaaa
    22081 ccgaagtttg cggcggcgac ttcagcgcca ccatcgaccg caccaaatgg ggcgtggact
    22141 acctcgttaa cgttggtatg accaaaagcg tccgcatcga catccaaatc gaggcagcca
    22201 aacaataaat cttgatgcga atcagcacaa atgccgtctt catcaaaccg ccccgccaac
    22261 ggtaagccga tatggcggag cacaatccgc cgaaagatat ttcagcggca ggcagggctt
    22321 gccggaaagt ccggaaa
//
LOCUS       DAWFBO010000015        21728 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFBO010000015 DAWFBO010000000
VERSION     DAWFBO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.015"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            66..617
                     /locus_tag="WGO87_000404"
     CDS             66..617
                     /locus_tag="WGO87_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391816.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(881..1430)
                     /locus_tag="WGO87_000405"
                     /pseudo
     CDS             complement(881..1430)
                     /locus_tag="WGO87_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1879..2436)
                     /locus_tag="WGO87_000406"
     CDS             complement(1879..2436)
                     /locus_tag="WGO87_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391817.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(2680..2838)
                     /locus_tag="WGO87_000407"
     CDS             complement(2680..2838)
                     /locus_tag="WGO87_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391818.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2835..3677)
                     /locus_tag="WGO87_000408"
     CDS             complement(2835..3677)
                     /locus_tag="WGO87_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0391819.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNGPADERVLLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKIQRPQGYRLLGIFEEGKHNAVAVCGFHEACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3915..4835
                     /gene="pyrB"
                     /locus_tag="WGO87_000409"
     CDS             3915..4835
                     /gene="pyrB"
                     /locus_tag="WGO87_000409"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0391820.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4845..5303
                     /locus_tag="WGO87_000410"
     CDS             4845..5303
                     /locus_tag="WGO87_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0391821.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5368..6033
                     /locus_tag="WGO87_000411"
     CDS             5368..6033
                     /locus_tag="WGO87_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391822.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6096..7313)
                     /locus_tag="WGO87_000412"
     CDS             complement(6096..7313)
                     /locus_tag="WGO87_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0391823.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7455..8000
                     /gene="def"
                     /locus_tag="WGO87_000413"
     CDS             7455..8000
                     /gene="def"
                     /locus_tag="WGO87_000413"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0391824.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            8087..9013
                     /locus_tag="WGO87_000414"
     CDS             8087..9013
                     /locus_tag="WGO87_000414"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0391825.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDAGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9092..10351
                     /gene="rsmB"
                     /locus_tag="WGO87_000415"
     CDS             9092..10351
                     /gene="rsmB"
                     /locus_tag="WGO87_000415"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0391826.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAILRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10317..10916
                     /locus_tag="WGO87_000416"
     CDS             10317..10916
                     /locus_tag="WGO87_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0391827.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10916..13033
                     /locus_tag="WGO87_000417"
     CDS             10916..13033
                     /locus_tag="WGO87_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0391828.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLISTASLSGNMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVETYA"
     gene            13026..14303
                     /locus_tag="WGO87_000418"
     CDS             13026..14303
                     /locus_tag="WGO87_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0391829.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14394..15581
                     /gene="dprA"
                     /locus_tag="WGO87_000419"
     CDS             14394..15581
                     /gene="dprA"
                     /locus_tag="WGO87_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0391830.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15607..16068
                     /locus_tag="WGO87_000420"
     CDS             15607..16068
                     /locus_tag="WGO87_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0391831.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16140..18446
                     /gene="topA"
                     /locus_tag="WGO87_000421"
     CDS             16140..18446
                     /gene="topA"
                     /locus_tag="WGO87_000421"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0391832.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18519..19214)
                     /locus_tag="WGO87_000422"
     CDS             complement(18519..19214)
                     /locus_tag="WGO87_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391833.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFQ"
     gene            19905..20282
                     /locus_tag="WGO87_000423"
     CDS             19905..20282
                     /locus_tag="WGO87_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0391834.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            20371..20940
                     /gene="rsmD"
                     /locus_tag="WGO87_000424"
     CDS             20371..20940
                     /gene="rsmD"
                     /locus_tag="WGO87_000424"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0391835.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21063..21314
                     /locus_tag="WGO87_000425"
     CDS             21063..21314
                     /locus_tag="WGO87_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0391836.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            21411..21494
                     /locus_tag="WGO87_000426"
     tRNA            21411..21494
                     /locus_tag="WGO87_000426"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21445..21447,aa:Tyr,seq:gta)
     gene            21525..21598
                     /locus_tag="WGO87_000427"
     tRNA            21525..21598
                     /locus_tag="WGO87_000427"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21557..21559,aa:Gly,seq:tcc)
     gene            21608..21682
                     /locus_tag="WGO87_000428"
     tRNA            21608..21682
                     /locus_tag="WGO87_000428"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21640..21642,aa:Thr,seq:ggt)
BASE COUNT         5359 a         5472 c         5995 g         4902 t
ORIGIN      
        1 agtttgtcct agttatctag gacagcccct tgttttttgt tcggcggctt gcgtggtcgg
       61 gtaaaatgaa agttttgaac ggttggtcgg acaggaagat gtggcgggtt ttgagtgctt
      121 tgccgatagg cgtggtgttt tttgatttga tctacggttt tgtgttgaat gtgttgcagg
      181 gtttggattt gcagcgtgcc gtgccggatt cggaaggcgt gttggcggtt acgcccgata
      241 ttgcattcaa cagtttgcag attgtcgcca acggcggtat ggcggcggtg gtctgtttcg
      301 ggttggcggt tgtgtttttg ctcaaccgtt cggtgcggcg gcggcaggtg ttggaaatcg
      361 gggtgttccg gatgttgggg ctggtggcgg tattggcgtt cagcgcgccg tcggtgtggg
      421 agtgggcgaa cgcgctgccg ctgctgctga agggcgcgga cgtggtcaat acggggaatg
      481 cgcgttatgt gctgacggct ttgtgtatgc cctttccggc ggtgtcgtgc gtcatcgggc
      541 tggtggggcg gttcaggctt cagacggcat cgggcagggc ggcaaagtca gggggtgcgg
      601 gcaaggcgga cggataggac gcatttttca gcgggtgcgt cgagaagcag ccgatgtgtt
      661 tggcagccgc agcttggggg ggtagtgcta atggcggttt ctttgctttt atagtggatt
      721 aacaaaaacc agtacggcgt tgcctcgcct tagcccaaag agaacgattc tctaaggtgc
      781 tcaagcacca agtgaatcgg tttcgtactg tttgtactgt ctgcagcttc gtcgccttgt
      841 cctgattact atataaaata aatgggcaaa aatcggttta ttatcgtttt tgccgcattt
      901 ggatttgttc taccgtaaaa cgtgtttgac gaacgggatt cttattaaaa aaacatctga
      961 tttctaacaa aatcagtatt ttttggcacg atggctaaaa tttttccttc catttcgcca
     1021 tcacgtgttt tccatgcgct caagaattgt gatttgctca ttgagacgtg ccccagcgat
     1081 ggatcagcca gcaaaacagt ttctccgtta ataccgttca ataccgaaaa atggttgttt
     1141 ttacggtatt ttaaatacac aattacagga atttttagtt gtaccaactg ttcaaatggc
     1201 aaagcataac cttgtgcttc aaaacccagt tcgggcatta tgcgttgcat atcatcaaaa
     1261 gaagcacgca tttgggtttt atccattttg tctaagattt ccgcttcaga ataatgtctg
     1321 ccataaaaat tattcagtaa cgtggcaatc gaagccgcgc cgcaagaaaa atccaaatct
     1381 tgttttacta tgccggaatc tcgccgtgct ttccaactcc gtacatggat gttttggtaa
     1441 gaagcggggg gtcaacaaac ataggccaag caaaaactat atttggggcg aaaccaatca
     1501 aagccgcata atttatcaat ttataaagat tttttatcat aatatgtata cgcggaataa
     1561 acgcatgaaa cataaaaaaa taaaatcata tgcaatttta ttgcatgaat aaatatgaat
     1621 aaaatagata atgatgggag taaatacgcc atgtattttg gaagtttaaa tttattaata
     1681 ataaaataat ttatcgtagc gcaaataaat cccaaaattg gaacgattag aaaaaaaatt
     1741 aatacaccca tcatcatgca aactctatat taataaatag ctaactaatt ctattgtgga
     1801 aattagttag ctaactaaaa gttattaatg attattttcg agaattgact gcattgttgg
     1861 cagcattggc accaaaacct agtgcatgaa tacccggtct ccatgccaaa ttcccagcca
     1921 atccgcctcc ggcagcagca gccagccctg ttttgccgct actcctgttg ccgcaccgat
     1981 tcctgccgca gtagccgcgc cttgcgcagt tcctaattta ccatgattat acaaattagc
     2041 accatgatac tcccatgcac ctaatgcacc gccaaaagca gcggctgcaa taatgggaac
     2101 aaattcacct tgtgtttctt tcatttcagc ctgtgataat tgaattgctt tcacattttg
     2161 gctgtcaaaa acttggctgt ctaaattttg cgccattaca ggtgtaatca tcatagccat
     2221 tacagttgca attttcgttg cgctggtttg cacataaata ggattagcaa attcgctttg
     2281 attgcgttca gtgttgatgt agctaatact gctttctagt ttgaatttac ccttgtcagt
     2341 caataattct tccaaactta aaggtaagtc agcataagca atttggctgg caacagttaa
     2401 aataaaatct attagacatt tgtgtttttg catcatttcg tttgattttc tagtttttga
     2461 gaatgataca aagtttttta caaagtaaag agtcactctg aaaaacattt tttcattata
     2521 aatcaaaata ttgatagaat aaatagcgag catcgattca cggtgcactt tagtgcaaag
     2581 cgtacaacgc gagcctgaac caccagccgc aacaggaaaa gaaagccgat agagtgcaat
     2641 gcttgccaac gtgcaagcga gcttgcaaga acgcttggct caacgagagc aggcaagaca
     2701 gaaagcagaa agcaggatag gagcggtaac gcaaaggtct cgggctttga tttcgccgta
     2761 aaccctgctg ccgccttgtc cggaaagggt gcaggcggcg agtgccgaca gggtgcagat
     2821 ggggaggggg gttttcattt ggggtcgcaa cggaagtggt atgcgcagat ttcaaaaccg
     2881 tttttgaaat acaggcggtg cgcgtcggca cggtcgtggt tgacgtggac gttgaggtgg
     2941 attttggtta cccctgtttc cgcgccgatt ttgcggactt cttccaaaag gcgcgaggcg
     3001 tagcctttgc ggcggctttg cggcagggta acgatgtcgt cgatgtggat gtggcggccg
     3061 ccggcgaggg tgcaggcttc gtggaagccg cagacggcga cggcgttgtg tttgccttct
     3121 tcaaaaatac ccagcaggcg gtagccttgg gggcgttgga ttttgttgat ttgttcggta
     3181 aagcggttga tgtcggtcag ggcggaacgc aaaacgctca aggctgcaaa ggcggtggcg
     3241 gtgtcgtccg cgccgatttc gcgcaaaacg taggatgcgc ccgaggcggt ctgttcctgt
     3301 gctttttcgg cggcgtgttt ttcttcgatt gcctgtgcca gcaggacgcg ttcgtcggcg
     3361 gggccgtttt gtccgccctg ttcgcgttct tcgagcaggg ctttgcagtc gatgacgcgc
     3421 aggtcgttgt cggcggcaaa gtccatcagg aagcggaaca tttggggatt gtcggtttcc
     3481 agttttttat cgacggcgac gcagcggatg ttgtccacca gcatggggcg cacccatttc
     3541 gagtaggaga ggcggttgtc tttggtgaac gaggacagga tgccgcacag gcgttccgcc
     3601 cagtcgctgg gacggaaaat cttgccggaa ctcgttgtgc cgtggatgac gacttcgtag
     3661 gggttgcaga ctaacatggc ggcttcctga aaagaaatgt ctggcgcgat tataccttat
     3721 gcttatgcgg gcgtgtttgg atatgccgtc tgaaaagtac gggattcgtg cggtaaaact
     3781 ttgcggcggc aaatgtgcga taatacgcgc cgtattgccg cttttgcgaa gctgttccgc
     3841 aaacatacgg gcggcgtgga cgacgtataa ccggataccc gcctgacgcg ggttttttac
     3901 ggaagggggc aaaaatgcct aatccgcttt acagacagca catcatctcg atttcggatt
     3961 tgtcgcgcga acagttggaa tgcctgcttc agacggcatt gaagctgaag gcgcatccgc
     4021 gcggcgattt gttggaaggc aaacttatcg gttcgtgctt ctttgaaccg tccacgcgca
     4081 cgaggctgtc gtttgaaacg gcggtgcagc ggctgggcgg caaggtcatc ggcttttcgg
     4141 acggcgcgaa taccagtgcc aaaaaaggcg agacgcttgc cgataccgcc cgcatcattt
     4201 ccggctacac cgacgcaatc atccaacgcc accccaaaga cggcgcggcg cgcgtggcag
     4261 cggagttttc gcgcgtcccc gtcatcaacg ccggcgacgg caccaaccag caccccagcc
     4321 agaccttgtt ggatctggta acgatttatg aaacgcaggg gcgtttggac aagctcaaaa
     4381 tcgccatggc gggcgacttg aaatacgggc gcaccgtgca ttcgctttgt caggcgttga
     4441 aacgctgggg ctgcgaattt gcctttgttt cgccgcccag cctcgcgatg cccgactaca
     4501 ttaccgaaga gttggacgaa gccggctgcc gataccgtat cctcggcagt ttggaagagg
     4561 cggcggaatg ggcggacatc ctgtatatga cccgcgtcca gcgcgaacgt ttcgatgaac
     4621 aggaatttgc caaaatccaa ggcaaattca atctcgacgc atccatgctt gcccgcgcca
     4681 aaccgaacct gcgcgtgctg caccctttgc cgcgcgtgga cgaaatccat cccgatgtcg
     4741 atgctacgcc gcatgcctat tatttcgagc aggcgaccaa cggcgtttat gcgcgtatgg
     4801 cgatattgtc gctggtgttg aacgaagaag tgtgaggaac cgatatggaa accccgaaac
     4861 tcagtgtcga agccattgaa aaaggtacgg ttatcgacca tattcccgcc ggcagggggc
     4921 tgaccatcct gcgccagttc aaacttttgc actacggcaa cgcggtaacc gtgggcttca
     4981 acctgcccag caaaacccaa ggcagcaaag acatcatcaa aatcaaaggc gtgtgcttgg
     5041 acgacaaagc cgccgaccgc ctcgccctgt tcgcccccga agcggtggtc aataccatcg
     5101 acaatttcaa ggtcgtgcag aagcggcatt tgaacctgcc cgacgaaatc gccgaagtgt
     5161 tccgctgtcc gaacacgaat tgcgccggcc acggcgagcc ggtcaaaagc cggttttatg
     5221 ttaaaaaaca caacgggcag acgcggctga aatgccatta ctgcgaaaaa acctacagcc
     5281 gggattcggt ggcggaagcc tgacggattc ccttaaaccg agcgggcggc atttcgtctg
     5341 ccgcttgttt tgccaatctg aaatggaatg atgatgcacg cttctgtcca aagccgtttc
     5401 gcaccgatac tttatgtttt gattttcttt gccggttttt tgaccgcgca aatctggttc
     5461 aatcagaaag cctatactga agagctgcct ccgcttctgt ccgcattgtc cgctgtcgcg
     5521 ctggtgtggc tggcgtgggc gttcgtgtcg gcgcgttcaa aggctaaggc ggaaaagttc
     5581 taccgcgaaa aaatgataca gaacgaaagc atacatcccg tcctgcacgc ttctttgcaa
     5641 cacttggaac acaagccgca aatgctcgcc ctgctggtca aaaaccacgg caaagggatg
     5701 gcggaacagg tcaggttcaa ggcggaagtg ctgcccgacg acgaagacgc gcgcacgatt
     5761 gccgccgagt tggcaaaaat ggatatgttt gcattgggga cggacgcggt cgcctcgggc
     5821 gaaacctatg gacgcgtgtt cgccgatatt ttcgagttgt cggcggcttt ggaagggcgc
     5881 gcgttcaaag gaatgttgaa actgacggcg gaatataaaa acatcttcgg cgatgcctgc
     5941 cgttcggaaa cggcgttgga gttgggcgcg ctcaatcagg cgttgcagga gatttcaaaa
     6001 acatcggaaa agtccaaacg gatattttat tgaagatgga aaaatgccgt ctgaaacgga
     6061 aggtgtttca gacggcattt ttgtcggatg attaattatt cggagcggtt gaagccaaac
     6121 ttcacgcggc tgcggccctg atccggtata ttgtccaaat cgcgtcccgg attggcggcg
     6181 gtgtcgccta cggaaatatc ggagatgttt tccaaaatga tggcggacga caggtgttcg
     6241 gaggtgcggt aaaccattgc caagcccact tcttcggcag gagtggaaat cagctcgacg
     6301 gtatccctgc ttttgaaatt gttggagagg tcgacctgca tcgttttctt gcgtttgtag
     6361 aggctcaaaa ccgtgccttt gtccaaaccg tccgcctcgc ctttgtcgat ggtgatggtt
     6421 tgaaactggc cggcaatcct tgtgccttca aacacggaaa cgattttagc ctgaaccggg
     6481 cgggacggtt cgtgcggcat catgttgaag cggtcggtgt cttccggcat tttcatcagg
     6541 tagtcgccct gctgtatttc ggaaatggcg gtttcgacca ccagcggctg tatcgaaggg
     6601 gtgcgcagcg gggtaatcaa aggatgggtg cgggtatggt attcgttgtc tttcggccgt
     6661 tctccagcct gtttcgagcg ttgttcgagg acagagtcgg tatagtcgag ggagcgcacg
     6721 atgccgctga atgcgacttc ctgcccgagg aatttacccg tatccggatc ggtgatgttt
     6781 ttattgattc ggtaggtcag gtagcggccc ggctctttca ggcctttggt gtaaaccctg
     6841 gtgcctttgg tgtacagcag cctgccttcc gggcccgaga gcaggcgcgg cgcggcagcg
     6901 gtttctttgc gggaaacgat ttgcgggtgc cgcataaaga tgcggtagaa gttgacatcg
     6961 atggcgggaa taccgtatcc ggacacttcc ttatccggac tcattttgac gacggggatg
     7021 ccgtctgtct gttccaagcc gaggcgcggt tcgccgtcaa cgtggcgcaa caccaatacc
     7081 tggtccggat aaatcaggtc gggattgtgg atttgatccc ggttcgcgtc ccacaggcgg
     7141 ccccattgcc acgggctgta caggtatttg cccgaaatgc cccacagggt gtcgccctgt
     7201 ttgaccgtgt agcgttccgg cgcgttcggg cgcacctcca aatttgccgc caaagtttgt
     7261 gttgagaatg ccatacctgc cgcgcagagc agggttataa tacgacgttg cataaccgtt
     7321 ccccttatct gataaatttc ggtttgtctt gcttgattgg gttggaaaaa gcggcggcag
     7381 cccctcggga tgtgccgcgt gataaaaaat gttccgcatt ttaacatcga attatccgca
     7441 ccatcacggt aattatgaaa aacaggcggc gtatccgccg aaggaaagag aaaattatgg
     7501 ctttattgaa tatcttgcaa tatcccgacg agcgtctgca cacggtggca aagcctgtcg
     7561 aacaagtcga cgagcgcatc cggaagctga ttgccgatat gtttgaaacg atgtacgaat
     7621 cgcgcggcat cgggctggcg gcgacgcagg tcgatgtgca cgagcgcgtg gtcgtgatgg
     7681 atttgaccga agaccgcagc gaaccgcgcg tgttcatcaa ccccgtcatc gttgaaaaag
     7741 acggcgaaac cacttacgaa gagggctgcc tgtccgtgcc gggcatttac gacaccgtaa
     7801 cccgcgccga acgcgtcaag gtcgaggctt tgaacgaaaa aggcgaaaag ttcacgctgg
     7861 aggcggacgg cttgctggcg atttgcgtgc agcacgagtt ggaccacctg atgggtatcg
     7921 tgtttgtcga acgcctttcc caactcaagc aggggcggat taagaccaag ctgaaaaaac
     7981 gtcagaaaca tacgatttga cccttttgcc gtgccgtctg aacgctgcaa agttttcaga
     8041 cggcacggtc ttgtccgaca attttacgca cacgcaggaa cacgctatga aagtcatctt
     8101 cgccggcacg cccgattttg ccgccgccgc cttaagagcc gttgccgccg ccggttttga
     8161 aatcccgctg gtgctgaccc agcccgaccg tccgaaaggg cgcggtatgc aactgactgc
     8221 cccgcccgtc aaacaagccg cgctggaact cggtttgcgc gtcgaacagc ccgaaaaact
     8281 gcgtaacaac gccgaagccc tgcaaatgct caaagaggtc gaggcagacg taatggtggt
     8341 tgccgcctac ggtttgattc tgccgcaaga cgtgttggat gtgccgaaac acggctgcct
     8401 caacatccac gcttcgctgc tgccccgttg gcgcggcgcg gcgccgattc agcgcgcgat
     8461 tgaggccggc gatgccgaaa ccggcgtgtg cattatgcag atggatgccg gtttggacac
     8521 cggtgatgtg gtcagcgaac accgctacgc catccaaccg accgataccg ccaacgaagt
     8581 ccacgacgcg ctgatggaaa tcggtgcggc ggcggttgtt gccgatttgc aacagcttca
     8641 aagcaaaggc cgtctgaacg cggtcaaaca gcccgaagaa ggtgttactt acgcgcaaaa
     8701 attgcgcaaa gaagaggcgc gtatcgattg gagcgaaagc gcggcggtta tcgaacgcaa
     8761 aatccgcgct ttcaaccccg tgcctgccgc gtgggttgag tatcagggca agccgatgaa
     8821 aatccggcgg gcggaagtgg tggcgcaaca aggcgcggca ggcgaagtgt tgtcctgttc
     8881 ggcggacggt ttggtcgttg cctgcggcga aaacgcgctg aagattaccg aattgcagcc
     8941 tgccggcggc aggcgtatga atatcgcggc gtttgcagca ggacggcata tcgaagcagg
     9001 gacgaagctg taaatccctt cagacggcat tccgatccgc aaacgggaat gccgcctgaa
     9061 accatcagtt gaagaaagcg aatcacataa tatgagtatg gcacttgccc aaaaacttgc
     9121 cgccgacagc attgcggcgg ttgccgaagg acgcaacctt caggacgtgt tggcgcaaat
     9181 ccgcaccgcg catcccgacc ttacggcaca ggaaaacggc gcgttgcagg acatcgccta
     9241 cggctgccag cgttatttgg gcagtttgaa acatatgctc gcgcagatgc tgaaaaagcc
     9301 gattgacaac ccgcagctcg aaagcctgct tttggcggcg ttgtaccaac tgcattacac
     9361 gcgcaacgcg ccgcacgccg tggtcaacga agcggtggaa agcatagcca aaatcggacg
     9421 cgggcagtac cgttcgtttg ccaacgcgat tttgcgccgc tttttgcgcg aacgcgacaa
     9481 gcttgcggct tcctgtaaaa aagacgatgt ggcgaaacac aacctgccgc tgtggtgggt
     9541 ggcttacctg aaaaaccatt atccgaaaca ctggcacaac atcaccgccg cgctacaatc
     9601 ccatccgccg atgacgttgc gcgtcaaccg ccgacacggc aatgccgaaa gctatttgga
     9661 aaaactggct gcggaaggta ttccggccag ggcgttggac gaatatgcgg ttacgttgga
     9721 agaagccgtg ccggtaaacc gtctgcccgg tttttcagac ggcattgttt cggtacagga
     9781 cttcggtgcg cagcaggcgg cgtatcttct taacccgaaa gacggcgaac ggattttgga
     9841 cgcgtgcgcc gcgccgggcg gcaagacggg gcatatcttg gaactggcgg attgccgcgt
     9901 taccgccttg gacatcgatg caggccgtct gaagcgtgtg gaggacaata tcgcccgttt
     9961 gggctttcag acggcatcgg cggcgtgtgc cgatgcgcgg gatttggcgg cgtggtatga
    10021 tggaaagcct ttcgacgcaa tccttgccga cgtgccatgc accgcttccg gcgtggcgcg
    10081 gcgcaatccc gacgtgaaat ggctgcgccg tccgaccgac gcgctcaaaa ccgcccgcca
    10141 gcaggaagcc ctgctggacg cactttggca gacgctgaaa cagggcggca gaatgctgct
    10201 tgccacctgt tccgtgttcg tcgaagaaaa cgacgggcaa ttgcaaaaat tcctcaaccg
    10261 ccatgccgat gccgaaccga tcgaatcgcg ggtgctttta ccgaacaaac accaagatgg
    10321 cttttattac gcgcttattc aaaagcatta aacaatggct tgtgctgctg ccgatgcttt
    10381 ccgttttgcc ggacgcggcg gcggagggga tagatgtgag ccgcgccgaa gcgaggataa
    10441 ccgacggcgg gcagctttcc atcagcagcc gcttccaaac cgagctgccc gaccagctcc
    10501 aacaggcgtt gcgccggggc gtgccgctca actttacctt aagctggcag ctttccgccc
    10561 cgataatcgc ttcttatcgg tttaaattgg ggcaactgat tggcgatgac gacaatattg
    10621 actacaaact gagtttccat ccgctgacca accgctaccg cgttaccgtc ggcgcgtttt
    10681 cgacagacta cgacaccttg gatgcggcat tgcgcgcgac cggcgcggtt gccaactgga
    10741 aagtcctgaa caaaggcgcg ctgtccggtg cggaagcagg ggaaaccaag gcggaaatcc
    10801 gcctgacgct gtccacttca aaactgccca agccatttca aatcaatgca ttgacttctc
    10861 aaaactggca tttggattcg ggttggaaac ctctaaacat catcgggaac aaataatgcg
    10921 ccgttttcta ccgatcgcag ccatatgcgc cgtcgtcctg ttgtacggac tgacggcggc
    10981 aaccggcagc accagttcgc tggcggatta tttctggtgg atagttgcgt tcagcgcaat
    11041 gctgctgctg gtgttgtccg ccgttttggc acgttatgtc atattgctgt tgaaagacag
    11101 gcgcgacggc gtattcggtt cgcagattgc caaacgcctt tctgggatgt ttacgctggt
    11161 tgccgtactg cccggcgtgt ttctgttcgg cgtttccgca cagttcatca acggtacgat
    11221 taattcgtgg ttcggcaacg atacccacga ggcgcttgaa cgcagcctca atttgagcaa
    11281 gtccgcattg aatctggcgg cagacaacgc ccttggcaac gccatccccg tgcagataga
    11341 cctcatcagc accgcctccc tatccggcaa tatgggcagg gtgctggaac attatgccaa
    11401 cgggtttaca cagttgtccc tttacaatgc cgcaagcggc aaaatcgaaa aaagcatcaa
    11461 cccgcacaag ctcgatcagc cgtttccgga taaggcgcgt tgggaaaaaa tccaacaggc
    11521 gggttcggtc agggatttgg aaagcatagg cggcgtattg tacgcgcagg gctggctgtc
    11581 ggcgggtacg cacaacgggc gcgattacgc cttgtttttc cgtcagccgg ttcccaaagg
    11641 cgtggcagag gatgccgtct taatcgaaaa ggcaagggcg aaatatgctg agttgagtta
    11701 cagcaaaaaa ggtttgcaga cctttttcct ggcaaccctg ctgattgcct cgctgctgtc
    11761 gatttttctt gcactggtca tggcactgta tttcgcccgc cgtttcgtcg aacccgtcct
    11821 atcgcttgcc gagggggcga aggcggtggc gcaaggcgat ttcagccaaa cccgtcctgt
    11881 tttacgcaat gacgagttcg ggcgtttgac caagttgttc aaccacatga ccgagcagct
    11941 ttccatcgcc aaagaagcag acgagcgcaa ccgccggcgc gaggaagccg ccagacatta
    12001 tcttgaatgc gtgttggagg ggctgaccac gggcgtggtg gtgtttgacg aacaaggctg
    12061 tctgaaaacc ttcaacaaag cggcggaaca gattttgggg atgccgctta cccccctgtg
    12121 gggcagcagc cggcacggtt ggcacggcgt ttcggcgcag cagtccctgc ttgccgaagt
    12181 gtttgccgcc atcggcgcag cggcaggtac ggacaaaccg gtccatgtga aatatgccgc
    12241 gccggacgat gccaaaatcc tgctgggcaa ggcaaccgtc ctgcccgaag acaacggcaa
    12301 cggcgtggta atggtgattg acgacatcac cgttttgata cacgcgcaaa aagaagccgc
    12361 gtggggcgaa gtggcaaaac ggctggcaca cgaaatccgc aatccgctca cgcccatcca
    12421 gctttccgcc gaacgtctgg cgtggaaatt gggcgggaag ctggatgagc aggatgcaca
    12481 aatcctgacg cgttcgaccg acaccatcgt caaacaggtg gcggcattga aggaaatggt
    12541 cgaagcattc cgcaattatg cgcgttcccc ttcgctcaaa ttggaaaatc aggatttgaa
    12601 cgccttaatc ggcgatgtgt tggcattgta tgaagccggt ccgtgccggt ttgcggcgga
    12661 gcttgccggc gaaccgctga tggtggcggc ggatacgacc gccatgcggc aggtgctgca
    12721 caatattttc aaaaatgccg ccgaagcggc ggaagaagcc gatgtacccg aagtcagggt
    12781 aaaatcggaa gcggggcagg acggtcggat tgtcctgacg gtttgcgaca acggcaaagg
    12841 gttcggcagg gaaatgctgc acaacgcctt cgagccgtat gtaacggaca aaccggcggg
    12901 aacgggattg ggtctgcccg tggtgaaaaa aatcattgaa gaacacggcg gccgcatcag
    12961 cctgagcaat caggatgcgg gcggcgcgtg tgtcagaatc atcttgccaa aaacggtaga
    13021 aacttatgcg tagcagcgat attttaattg tagacgacga aatcggcatc cgcgacctgc
    13081 tgtcggaaat cctgcaggac gaaggttatt cggtcgcatt ggcggaaaac gccgaagagg
    13141 cgcgcaagct gcgccatcag gcgcgtccgg cgatggtgct gctggatatt tggatgcccg
    13201 attgcgacgg catcaccctt ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg
    13261 tggtgatgat gagcgggcac gccagcatcg ataccgccgt ggaagccacc aaaatcggcg
    13321 cgatcgattt tttggaaaaa ccgatttccc tgcaaaagct gctgtctgcc gtcgaaaacg
    13381 cgttgaagta cggtgcggcg caaaccgaaa cggggcctgt attcgacaag ctgggcaaca
    13441 gtgcggcgat tcaggaaatg aaccgtgagg taggggctgc ggtgaaacgt gcctctcccg
    13501 tacttttgac gggcgaggca ggttcgccgt ttgaaacggt ggcacgctat ttccataaaa
    13561 acggtacgcc gtgggtcagc ccggcaaggg tcgaatatct gatcgatatg ccgatggaac
    13621 tgttgcagaa ggcggagggc ggcgttttgt atgtcggcga catcgcccag tacagccgca
    13681 acatccaagc cggtattgcc tttattgtcg gcaaggcgga acaccgccgc gtcagggtgg
    13741 tcgcatcggg cagcagggcg gcaggttcag acggcattgc ctgcgaggaa aagctggcgg
    13801 aactgctgtc ggagtcggtc gtccgcattc cgccgctgcg tatgcagcat gaagatattc
    13861 ccttcctgat acaggggatt gcctgcaatg tggcggaaag tcaaaagatt gcgcccgccg
    13921 cattcagtga agaggcactt gccgcattga cccgttacga ctggccggga aatttcgacc
    13981 aactgcaaag cgtcgttgca acgctgttgt tggaggcgga cggacaggaa atcggcgcgg
    14041 cggcggtttc ttcccttttg gggcagaatg tgcctgccga gggagcggaa gatatggtgg
    14101 gcgggtttaa tttcaacctg cccctgcgcg aattgaggga ggaggtggag cggcgttatt
    14161 tcgagtacca catcgcccaa gaaggtcaga atatgagcaa agtggcgcag aaggtcggtt
    14221 tggaacgcac acacctttac cgcaaactca aacagctcgg catcggcgtt tcgcgccggg
    14281 cgggggaaaa aaccgaagaa taggcccgga cggtcggttt tccggctgcg ggcttttgtt
    14341 ttcagacggc atttggtgca aatgccgtct gaaatcgtaa ggggacggat tttatgacag
    14401 aggacgaacg tttcgcgtgg ttgcaactgg cttttacgcc ctatatcggt gcggaaagtt
    14461 tcttgctgct gatgcgccgt ttcggcagcg cgcaaaatgc cttgtccgca ccggcggaac
    14521 aggtcgcccc ggcggtgcgg cacaagcagg cggccgaggc ttggcgcaat gcggaaaaac
    14581 gcgctttggc gcggcaggcg gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc
    14641 gcctgatgct gcttcaggat gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc
    14701 cgccggtttt gtttttgcgc ggcaacgtgc ggctgctgca caagccttcc gccgccatcg
    14761 tcggcagccg ccatgccacg ccgcaggcga tgcggattgc caaagatttc ggcaagtcgt
    14821 tgggcgggca aaatatcccc gttgtgtcgg gtatggcttc cggtatcgat actgccgccc
    14881 atcagggcgc actggaggca gaaggcggca ccatcgccgt gtgggggacg ggtatagacc
    14941 gcatttaccc gccgtccaat aaaaaccttg cctatgaaat cgccgaaaaa ggattgattg
    15001 tcagcgagtt tcccatcggt acgcgcccgt atgccggcaa ctttccgcgc cgcaaccgcc
    15061 tgattgccgc gctgtcgcag gttacgctgg tggttgaagc tgcgttggaa tccggttcgc
    15121 tgattaccgc cggcttggcg gcagaaatgg ggcgcgaagt gatggcagta cccggttcga
    15181 tagacaatcc gcacagcaaa ggctgccaca aactgattaa agacggcgcg aaattggtgg
    15241 aatgcctgga cgacatcctg aacgaatgcc cggggctatt gcaaaatacg ggtgcttcat
    15301 catattctat aaataaggat acgcccgaca cgggacgccg cgccgttcag acggcatacg
    15361 ccccgccgcc tgccgcaaaa atgccgtctg aagcggcggc aggcggcaca gccgtcggcg
    15421 gcatactgga taaaatgggt ttcgacccca tccatcccga cgtgcttgcc ggacagttgg
    15481 ctatgcctgc cgcagatttg tatgccgcac tgttggaatt ggaattggac ggcagcgttg
    15541 ccgcaatgcc cggcggcaga taccagcgta tccgaacttg aacgcacttt atattaagga
    15601 acacgaatga ccgaagtcat cgcctacctc atcgaacatt tccaagattt cgatacctgt
    15661 ccgccgcccg aagacttggg tatgctgctt gaagaagcgg gtttcgatac gatggaaatc
    15721 ggcaacaccc tgatgatgat ggaagtattg ctcaacagct ccgaattttc cgccgaacct
    15781 gccgacagcg gcgcattgcg cgtgtacagc aaagaagaaa ccgacaacct gccgcaggaa
    15841 gtgatggggc tgatgcagta tctgattgaa gaaaaagccg tcagctgcga acagcgggaa
    15901 atcatcatcc acgcgctgat gcacatcccc ggcgacgaaa ttaccgtaga taccgccaaa
    15961 gtgctgaccc tgctgctttt atgggcaaac aagagcgagc tgcccgtgtt ggtcggcgac
    16021 gagctgatga gcgcgctttt actcgacaac aaacccacga tgaactgaag cggcttcaga
    16081 cggcccgccc gaggccgtct gaaacgccgg catcaaaacc accatccaga gaacgacaaa
    16141 tggcgaaaaa cctattaatc gtcgaatccc cgtccaaagc caaaaccctg aaaaaatatt
    16201 tgggcggcga ttttgaaatc cttgcatcct acggacacgt ccgcgacctc gtccccaaaa
    16261 gcggcgcggt cgatcccgac aacggctttg cgatgaaata ccaactcatc agccgcaacg
    16321 gcaaacacgt cgatgccatt gtcgccggtg ccaaagaagc tgaaaacatc tacctcgcca
    16381 ccgacccgga tagggaaggc gaagccattt cctggcatct tttggaaatc ctcaaatcca
    16441 aacgcggctt gaaaaacatc aagccgcagc gtgtcgtgtt ccacgaaatc accaaaaacg
    16501 ccgtgctcga tgccgttgcc catccgcgcg aaatcgaaat ggacttggtc gatgcgcaac
    16561 aagcccgccg tgctttggac tatttggtcg gctttaacct ttcgccattg ttatggaaaa
    16621 aaatccgccg cggtttgagc gcgggtcgtg tgcaaagtcc cgctttgcgc ctgatttgcg
    16681 aacgcgaaaa cgaaatccgc gcgtttgaag cgcaggaata ttggacggta catctagaca
    16741 gccacaaagg ccgcagcaag ttcaccgcca aactcgccca atacaacggc gcgaaactcg
    16801 aacaattcga cctgccgaac gaagccgctc aagccgatgt gttgaaagaa ctcgaaggca
    16861 aagaggccgt cgttaccgcc atcgaaaaga aaaagcgcag ccgcaatcct gccgcgccgt
    16921 tcaccacttc taccatgcag caggatgccg tgcgcaaact cggcttcacc accgaccgca
    16981 ccatgcgtac cgcccaacag ctttatgaag gtatagacgt agggcagggc gcgatcggtc
    17041 tgattaccta tatgcgtact gacagcgtga atttggcgga tgaagcgtta accgaaatcc
    17101 gccattacat cgaaaacaaa atcggcaaag aatatctgcc gagtgccgcc aaacaataca
    17161 aaaccaaatc caaaaacgcc caagaagcgc acgaagccat ccgtccgact tccgtgtacc
    17221 gcacgcccga aagcgtcaaa cccttcctga gcgcagacca gttcaaactc tatcaaatga
    17281 tttggcagcg taccgtcgcc tgtcagatga cgcctgccaa attcgaccaa accaccgtcg
    17341 atattaccgt cggcaaaggc gtattccgcg taaccggaca agtgcaaacc ttcgcaggct
    17401 tcttgagcgt ttatgaagaa agcagcgacg atgaagaagg cgaagacagc aaaaaactgc
    17461 ccgaaatgag cgaaggcgat aaattgcccg tggacaaact ctacggcgaa caacacttta
    17521 ccactccgcc gccgcgctac aacgaagcca cgctggttaa agccctcgaa gaatacggca
    17581 tcggccgccc ctcgacctac gccagcatca tctccacgct caaagaccgc gaatacgtta
    17641 cccttgagca aaaacgcttc atgcccaccg acacaggcga catcgtcaac aaattcctga
    17701 ccgaacactt cgcccaatac gtcgattacc acttcaccgc caaactcgaa gaccagcttg
    17761 acgaaattgc cgacggcaaa cgccaatgga ttcccttgat ggacaaattc tggaaaccgt
    17821 tcatcaagca agtggaagaa aaagaaggca tcgaacgcgc caaatttacc acgcaggaac
    17881 ttgatgaaac ctgcccgaaa tgcggcgaac acaaactgca aatcaaattc ggcaaaatgg
    17941 gtcgttttgt tgcgtgtgcc ggttatcccg agtgcagcta cacgcgcaac gtcaacgaaa
    18001 ctgccgaaga agccgccgag cgcatcgcca aagccgaagc cgaacaggcc gaactcgacg
    18061 gacgcgagtg cccgaaatgt ggcggtcgcc tagtgtacaa atacagccgc accggcagca
    18121 aattcatcgg ctgcgccaac taccccaaat gcaaacacgt cgagccgctg gaaaaaccga
    18181 aagataccgg cgtccagtgt ccgcaatgca aaaaaggcaa cctcgtcgag cgcaaatccc
    18241 gctacggcaa actgttttac agttgcagca cctatcccga ctgcaactac gccacttgga
    18301 acccgcccgt tgccgaaagc tgtccaaaat gcgcatggcc ggttttgacc atcaaaacca
    18361 ccaaacgctg gggtgtggaa aaagtttgtc cgcaaaaaga atgcggctgg aaagaacaga
    18421 ttgaaccgcc tgcaccgaaa gagtgagatt aggttggttt gaaagagaaa aggtcgtctg
    18481 aaaaattttc agacgacctt tgcttttctg tgattggttt attggaatcc gcgtgttgtt
    18541 ttaaagtccg ataaaatccg gttcatttca ggcgcaaaca aggcgatgta atcgtaagat
    18601 agaccgcgac tggcactggg atggggaaag cagacgactt cgcaatcttc aaacgattgg
    18661 aatttgacat tgaaacgtgt accgtcaaat tctttttgca ccgtctccag cggtttggtc
    18721 tgcttaccga ccaactgctc gaagcgtggc agtacatttt ggttgttcag aaaatccgcc
    18781 aacctgctgc ccatgaagag gatgactttc ggacgcagtt tttcgatgtg gtagagaaaa
    18841 ttatcgatgt gctcgggttg tgtgaacttg tcgggattgt cgatagtgtt gccctgtgta
    18901 gcagcccagt tggtttgaac cagggatttt tcaaatgcac cgcccaatcc attttcgtct
    18961 aaggggtgtc cccacatttc aaaccaattt tttatcgtat tatcgtaacg ccactttttt
    19021 gcctgctctc cgaaatagag ggatttgttt gcaaatgtat ggtcgatttt gttttcaggg
    19081 agtttgtatt cacctgctac ataagcagcc tcatcggctt tactccaacc ccattcatag
    19141 ccacaaatca ttaagccatg tttgtcgttg tagcctttga acaggctgtt gctcaaattc
    19201 aaatccttca tcatgaactc ttccttttaa aatttaagag cgattgactt caatgttttt
    19261 agatggggtg gaaaaatcct tgtgtaggca acataaattc aataaatttc ttgataattc
    19321 gaaacctact aatagcgcac ctataaaagc tttttcatta cgttcagcat gacggtcacg
    19381 tcgttcatat tttttacgct tgctgttccc tgttattaca gctaagccaa gtgatatggc
    19441 gagaattgcc caaacaatag tacttaaata acaattttcc ccatactatc aataaggaaa
    19501 gaaaaaacct tttagtatta ggtcgataga ttataatcca tgcccatgaa aatgcaataa
    19561 gagttataca taagatacaa gctgacagga tttctttatt tttaattaaa taacttagag
    19621 cgatgaggat gacaggtgtc aagaaaataa tagttacatc ccgatagcta taaaagaaaa
    19681 ctgccctatt ttgaatgtgg agatgtgcac agaatccaat atagctaagg ataatagtta
    19741 atataataaa aaaagaccac caagggtgaa gagataggaa ttccatgttt tccctttttt
    19801 tgtaaaaagg aaaaaatctt atctaatagt taattgccta accagccaga agaagtttaa
    19861 aatctatccc aataattcaa ccatctatac agaaagttca gcttatggaa acccacgaaa
    19921 aaatccgcct gatgcgcgaa ttgaataaat ggtcccagga ggatatggcg gaaaagctgg
    19981 cgatgtcggc aggagggtat gccaaaatcg aacggggcga aacgcagttg aatatcccgc
    20041 gtttggagca gttggctcag attttcaaaa tcgatatgtg ggacttgctc aaatcgggcg
    20101 gtggtgggat ggtgtttcag attaatgaag gtgatagtgg tggcgatatt gcgttgtatg
    20161 cgtcgggtga tgtttcgatg aaaatagaat ttttaaaaat ggagttgaaa cactgcaaag
    20221 aaatgttgga acaaaaagac aaagaaatcg agctgctccg caagctgacc gaaaccgttt
    20281 aaacagatat gccgtctgaa aaaagttttc agacggcata ttctttgaca ggtcttgtat
    20341 aataccgttt gaacttacag gtttttgatt atggcggcag gcaaacatac caaacacagc
    20401 aaccgggtac gcattatcgg cgggcaatgc cggggcagga aattgagttt cacatccgcc
    20461 gacggactgc gtccgacacc cgacagcgtg cgtgaaaagc tgtttaactg gctgggacag
    20521 gatttgacgg gtaaaacggt tttggatctc ttcggaggca gcggcgcact cggtatagaa
    20581 gccgcttcgc gcaacgccaa acgcgtgctg atttcggata acaaccgcca aaccgtgcag
    20641 accttgcaga aaaacagtcg cgaactgggt ttggggcagg tgcaaatcgt cttttcagac
    20701 ggcatcgcat atttgaagac cgtatccgaa cagtttgatg ttgtctttct cgacccgccg
    20761 tttgcatggc aggactggca aatcctgttt gatgccttga agccgtgcct gaacccccgg
    20821 gcattcgtct atctcgaggc gggtacgctg ccgaatattc ccgattggct gacggaatat
    20881 agagaaggga aatcggggca gagtacattt gaattaaggg ttttccaagt ggctgaataa
    20941 tatgcgcttt gataatcatt tccgagttgt aaacattcgt ttgcaaccgt ccggttcaaa
    21001 aaaaccttgt gctataatcc gcgcccgccc ggttttgata atttagtgga aaaggaaaag
    21061 aaatgtcgct ttttattacc gacgagtgca tcaactgcga cgtatgcgaa cccgaatgcc
    21121 ccaatgatgc catttcccaa ggcgaggaaa tttacgaaat caaccccaac ctctgcacgc
    21181 agtgcgtcgg acactacgat gagccgcagt gccagcaggt ttgcccggtg gactgcatcc
    21241 tgattgacga agaacatccc gaaacccatg acgagttgat ggcgaaatac gaaaagatta
    21301 tccagtttaa ataaattctt tttaaaacat caaattatgt ctgttttgaa ataaaatcaa
    21361 aaaaaaactt gacggaaaag caagccgcta ataaactaac gttctctttt ggagggattc
    21421 ccgagcggtc aaagggggca gactgtaaat ctgttgcgaa agcttcgaag gttcgaatcc
    21481 ttctccctcc accaaaattc ttacttgggg cagtagcgag taatgcgggt gtagctcaat
    21541 ggtagagcag aagccttcca agcttacggt gagggttcga ttcccttcac ccgctccaaa
    21601 caattaggcc catgtagctc aggggtagag cactcccttg gtaagggaga ggccggcagt
    21661 tcaaatctgc ccatgggcac catctctcga ttattcattt ctttaaggct tagatatata
    21721 ggatattg
//
LOCUS       DAWFBO010000016        21723 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFBO010000016 DAWFBO010000000
VERSION     DAWFBO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.016"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(84..1970)
                     /gene="uvrC"
                     /locus_tag="WGO87_000429"
     CDS             complement(84..1970)
                     /gene="uvrC"
                     /locus_tag="WGO87_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0391837.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(2025..4202)
                     /locus_tag="WGO87_000430"
     CDS             complement(2025..4202)
                     /locus_tag="WGO87_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0391838.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVKSAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4341..5291
                     /gene="trxB"
                     /locus_tag="WGO87_000431"
     CDS             4341..5291
                     /gene="trxB"
                     /locus_tag="WGO87_000431"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0391839.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5447..5815
                     /gene="rpsF"
                     /locus_tag="WGO87_000432"
     CDS             5447..5815
                     /gene="rpsF"
                     /locus_tag="WGO87_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0391840.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5816..6118
                     /gene="priB"
                     /locus_tag="WGO87_000433"
     CDS             5816..6118
                     /gene="priB"
                     /locus_tag="WGO87_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0391841.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6125..6355
                     /locus_tag="WGO87_000434"
     CDS             6125..6355
                     /locus_tag="WGO87_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0391842.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6372..6824
                     /locus_tag="WGO87_000435"
     CDS             6372..6824
                     /locus_tag="WGO87_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0391843.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            6838..6945
                     /locus_tag="WGO87_000436"
     CDS             6838..6945
                     /locus_tag="WGO87_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0391844.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            7530..7685
                     /locus_tag="WGO87_000437"
     CDS             7530..7685
                     /locus_tag="WGO87_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391845.1"
                     /translation="MYTGHYGGNTVWEAAASRGIGTAGDAPMPSGVKAGQSAVWKKAM
                     ESVGRRS"
     gene            complement(7801..8607)
                     /locus_tag="WGO87_000438"
     CDS             complement(7801..8607)
                     /locus_tag="WGO87_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0391846.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(8608..9354)
                     /gene="pssA"
                     /locus_tag="WGO87_000439"
     CDS             complement(8608..9354)
                     /gene="pssA"
                     /locus_tag="WGO87_000439"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0391847.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            9937..10419
                     /locus_tag="WGO87_000440"
     CDS             9937..10419
                     /locus_tag="WGO87_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ0391848.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(10471..11682)
                     /locus_tag="WGO87_000441"
     CDS             complement(10471..11682)
                     /locus_tag="WGO87_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0391849.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            11905..14343
                     /locus_tag="WGO87_000442"
     CDS             11905..14343
                     /locus_tag="WGO87_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0391850.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            14552..15862
                     /locus_tag="WGO87_000443"
     CDS             14552..15862
                     /locus_tag="WGO87_000443"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0391851.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKVPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQVPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15958..16572
                     /gene="clpP"
                     /locus_tag="WGO87_000444"
     CDS             15958..16572
                     /gene="clpP"
                     /locus_tag="WGO87_000444"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0391852.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQL"
     gene            complement(16626..17891)
                     /gene="ispG"
                     /locus_tag="WGO87_000445"
                     /gene_synonym="gcpE"
     CDS             complement(16626..17891)
                     /gene="ispG"
                     /locus_tag="WGO87_000445"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0391853.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKITLKGDNIASEFL
                     AIVEEYVKTNYGENSSKRNKGKVIPIQSL"
     gene            complement(17907..18668)
                     /gene="pilW"
                     /locus_tag="WGO87_000446"
     CDS             complement(17907..18668)
                     /gene="pilW"
                     /locus_tag="WGO87_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0391854.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18671..19765)
                     /gene="rlmN"
                     /locus_tag="WGO87_000447"
     CDS             complement(18671..19765)
                     /gene="rlmN"
                     /locus_tag="WGO87_000447"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0391855.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMIPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19909..20334)
                     /gene="ndk"
                     /locus_tag="WGO87_000448"
     CDS             complement(19909..20334)
                     /gene="ndk"
                     /locus_tag="WGO87_000448"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0391856.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPAEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(20393..21544)
                     /locus_tag="WGO87_000449"
     CDS             complement(20393..21544)
                     /locus_tag="WGO87_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0391857.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKDSGG"
BASE COUNT         5200 a         5369 c         5905 g         5249 t
ORIGIN      
        1 ggtttaaatt taatccacta tatatcggta taccgacctg ctgtatcata ttttttacag
       61 acggtttttg cttgggcagc atactaatgc agatgctcgt aaatggtttc cgccaatgcc
      121 ttgctgatgc cttccacttt ttccaagtcc tcgcggctgg cggcaatcac gccgcgcaga
      181 ccgccgaagc gggtgagcag ggcttggcgg cgtttgctgc ctacgccggg gatgtcgctt
      241 aaggaggagg taacgcgggc tttgtcgcgt tttttgcggt ggccggtaat ggcgaaacgg
      301 tgcgattcat cgcgtacggt ttgcaataga tgcaaggccg ggctgttggg cggcaggcgg
      361 aagacttcgc cggtaaaagg cagtatgagt tcttccatac cggctttgcg ctccgggcct
      421 ttggcgatac cgaccaaagg aatgtgcagc ccgagttctt cccataccga tacggcgatg
      481 ccgatttgcc ctttgccgcc gtcaattaac acggcatccg gccatttgac ggtctcgccg
      541 ttggcttcgg cttcctgcat tttgccgtaa cggcgcgtca acacttcgcg catggcggcg
      601 tagtcgtcgc cgggtttggc ggtcgtgatg ttgtagcggc ggtattgcga gggctggatg
      661 ttttgctcat cgtacacgac gcaggaggcg atggtggctt cgccttgggt gtggctgatg
      721 tcgaagcatt cgaggcggtt gaggccgtct gaatccatac cgaggatttt ggcgagttcg
      781 tcgatgcggt gttgctggct gctttgttgc aggcggcgtt gtgcaatcgc catttgtgcg
      841 ttttgctccg ccattttcaa ccaaactttg cgttcgccta tggtcttggt gacaaattgc
      901 atttgtttgc cgtgttcgct ctctagggct tctttcaatg cgtcgggcat ggggaagttg
      961 ctgatgatga tgtcaggttt gcttttgccc agatagtgtt gggcgacgaa ggcttcggca
     1021 tagtcttgcc catttggctc gggatcgttt ttggtgtcgg ggaagaaact tttgtctccg
     1081 acgtgccgcc caccgcgtat gctgacccag tgtacgcaga cgatgccgtc tgaaaccgcc
     1141 agcgcgagca aatcgatatc gttggggttg ttcgggtttt tgctgtcgat gaactggttg
     1201 ctctgcataa tgccgagtgc ttggatttgg tcgcggtagc gggcggcttc ttcaaattgc
     1261 agattagcgg cggcagtttg cattttgtgt tgcagggtgc gggtcagttc gtcggttttg
     1321 ccattgagaa aagtggcggc ttgacgcacg ctgtcgcggt agtcttcttc gctgatgtgt
     1381 ccgacgcagg gcgcggtgca gcgtttgatt tgatacagca ggcaagggcg gtcacgatgc
     1441 tcgaatacgc tgtcttcgca ggtacgcagc ataaagactt tttgcaacac ttgaatgctg
     1501 tcgcgcacgg cgttgctgtt gggatacggg ccgaagtatt ggtttggctt tttcagcgtg
     1561 ccgcggtaat acgccatctg cggatattga tggccgctga gcatcaaata aggatagctt
     1621 ttgtcgtcgc ggaaaaggat attgtatttc ggcgacaagg ctttgatgag gttgttttcg
     1681 agaatcaggg cttcggcttc ggaacgcgtg atggtggttt cgatatgatg aacctgtttt
     1741 accatcaatg cgatgcgcgg cgaatggtcg tttttttgga aatagccgga cacgcgccgc
     1801 ttgaggttga ccgctttgcc gacgtataag actttgccgc cttcgtcaaa aaaacggtac
     1861 acgcccggca gcttgggcag gtttttgagg aagagcggga tatcgaattg ttccggagaa
     1921 cggttttctg tattcatatc aattatttga aaaacggctt cagacggcat atcggcagcg
     1981 gcagccttat ccgttcggaa aagctgtcgg gtggttatat gcggtcaatc gatttttacc
     2041 cgtttcaggc gcaaggcgtt gctcaacacg gaaaccgagc ttgccgccat tgccgcgcct
     2101 gcgatgacag ggtttaaaaa accgagcgcg gcaagcggaa tgcccaaaat attgtagaag
     2161 aaggcgaaaa acaggttttg cttgatgttt ctcaacgtcg cccgcgatac cgatagggca
     2221 tcggcgagtt ggttgaccga atgttgcatc agcgtggcgg atgcggtgtg ttcggcaacg
     2281 tccgcgccgc ctttcatggc gaagctaacg ttggcggcgg cgagcgcagg tgcgtcgttg
     2341 atgccgtctc cgaccatcgc cacggttttg ccggcggctt tgagtttctg cacttcggcg
     2401 gctttgtcgc gcggactcat attaccgaag gcgtgtgcga tgcccagttg tttggcgacg
     2461 tattcgaccg tgccttggtt gtcgccgctc ataatataga catcgatatt gtgttttttc
     2521 agacggccta tggcttcggc ggtatcggct ttcaacgcgt cggcaagtgc gaatgcgccg
     2581 atgggtttgt tatcgactga gaccgcaaca atgcttgcaa tatcccaaac gccgtctaaa
     2641 aacttcggca aggccagttc ggcaaattcg gctttgcctg ctttcaccaa acccacgcct
     2701 tccacttcgg cggtaatgcc tgcgccgaca acggtttgtg cgttttgtgc ggcgggaatg
     2761 tccaaaccgc gcgcttgggc ggcggagacg atggcacggg cgagcggatg ggcggcgttt
     2821 tgttcgacgg cggcggcgat gcggtacaaa gcgtcttcgt caaagccgct gtcgggaacg
     2881 caataaacgg cggcaacctg cgggctgcct tcggtcagcg taccggtttt gtccaacacg
     2941 acggcatcga cgtgggcggc ttcctccatt gctgccgcgt ctttaaacca aataccgtgt
     3001 ttcaccgctt tgccaatgcc gaccataatc gcggcgggtg tagccagacc gagcgcacac
     3061 gggcaggcaa tcaccaaaac ggcgacggcg tgcatcagcg caaccgtcca atcgccctta
     3121 atcagccaag taacaataaa agtcaacaac gcgatgccca cgacggcagg cacgaacacc
     3181 gccgccgcct tgtccgccac gcgcgcaatc ggtgctttac tgccttgcgc ttcagagagt
     3241 gcgttcatca tgtcgccgag cagggtttgg ctgccgagct gcgcggcgcg gtacaccacg
     3301 ctgccttcag tcatcagcgc gcccgccaat actttgccgc ctgccttttt ctcttcggga
     3361 ttggattcgc cggtaagatg gctttcgtcc gcccagccgc tgccgctttc tatgatgccg
     3421 tcggcggcaa tgcgttcgcc gtgattggcg cggattaggt cgccgatttg cacttggtcg
     3481 atgggtagct gccgccattc gccatcgcgt tgcacgttga cttgggttgg cgtgagtttg
     3541 agcagcaagc ccaagctgtt caggctggat tttttggtgc ggtgttccaa aaatttaccc
     3601 agtgacacaa aaccaatcac cattatgcct acttcaaaat acacatgcgc cataccgtac
     3661 gccgcgtgcg ggctgaaaaa cagcatatag acggaataca ggtaaatcga gaccgtgccg
     3721 atggtaacga gtacgtccat attcgccagc ccgcctttaa tgctcgccca cgcgcttttg
     3781 taaaatggca ccgccagcca aagctgcacc acactcgcca atgcaaactg caacaagggc
     3841 gacaacatcc aatcatgccg tgtccaattc agccctttta gcatcatccc taccataccg
     3901 ataaggaacg ggatattgat ggccagcaaa agccacaacc tccagccgat atggtgttct
     3961 gcttcgggtt gcggcaatgt atcttccgtc ttttccttcg cgccgtaacc ggttttctca
     4021 atgattttgg caatgtcggc agcggaagtt ttgctgtcgt caaacgtaac ctgcgcctcc
     4081 tcgctggcga agttcactcc cgctgatttg acaaagtctt ttttgttcaa cactttttca
     4141 atgcgcgaag cacatgcctg acaggtcatg ccttcgattt ggaaacggat tttttgttgc
     4201 ataagcgtga ttcctgcttt gatattcaga cggcagttaa ggctgcggga tgccgtctga
     4261 gcgccggtat ggtcgaaatt tctcaaacgc gccgataatt cgagtagaat ccgaaaactt
     4321 gacacagcaa aggaaaaaca atgtcccaac accgcaaact gattattttg ggttccggcc
     4381 ccgccggata caccgccgcc gtctatgccg cgcgtgccaa tttaaacccc gtcattatta
     4441 caggtatcgc gcaaggcggg caactgatga ccaccaccga agtggacaac tggcctgccg
     4501 atgccgacgg cgtgcaaggg ccggaattga tggcgcggtt tctcgcccac gccgaacgtt
     4561 tcggaacgga aatcattttt gaccaaatca acgccgtcga tctgcaaaaa cgcccgttta
     4621 cactcaaagg cgatatgggc gagtacactt gcgatgccct gattgtcgca acaggcgcgt
     4681 ccgccaaata cctcggtttg ccgagtgagg aagcgtttgc aggaaaaggc gtttccgctt
     4741 gtgccacctg cgacggtttc ttctacaaaa accaagatgt tgccgtagtt ggcggcggca
     4801 atacggcagt tgaagaggca ctctaccttg ccaatatcgc caaaaccgtt accctgatcc
     4861 accgccgcag cgagttccgt gccgaaaaaa tcatgatcga caaactgatg aaacgcgtgg
     4921 aagagggcaa aatcatcctc aagctggaaa gcaacctgca agaagtactg ggcgacgatc
     4981 ggggcgtaaa cggcgcatta ttaaaaaaca acgacggttc tgagcaacaa attgccgtca
     5041 gcggcatttt tatcgccatc gggcacaagc cgaataccga tattttcaaa ggtcagttgg
     5101 aaatggacga agccggttac ctgaaaacca aaggcggcac ggcggacaat gtcggcgcaa
     5161 ccaatatcga aggcgtatgg gcggcgggcg acgtaaaaga ccatacctac cgtcaggcga
     5221 ttaccagcgc ggcttccggc tgtcaggcgg ctttggacgc ggaacgctgg ctgggcagcc
     5281 aaaacatttg aatgccgtct gaaaccgaaa ttttcggcgg aatgcaagtt tttcgcacca
     5341 aatccttgat tccgtcggca aaccccaata taatgcccgt tctttgaacc ttgccttctg
     5401 ctttcgcatg gcagaaggct gtttttacca tccataagga gataacatgc gtcattacga
     5461 gatcgtgttt atcgttcatc ctgatcaaag cgagcaagtg cccgctatgg ttgaacgtta
     5521 caaaaccatg attgctgaag caaacggtaa gattcaccgt ttggaagatt ggggtcgccg
     5581 ccaactggct tacccgatta acaaaatcca taaagcccat tacgttttga tgaacatcga
     5641 aaccactccc gaagtggttg aagagctgga aaccgcattc cgcttcaatg atgccgtatt
     5701 gcgccatcta accatcaaaa ccaaacacgc cgttaccgaa gcatccccta tgttgggtgg
     5761 tgaaaaggct aagaacctgt tgagcggtgc gtctgaagaa gcggtcgccc aataattggg
     5821 attcaataat cttgtttcgc ttgccgcgtt aattgaaaag gttttcccta ttcgatatac
     5881 gcctgccgga atccctgttt tagatattat tttaaagcac aaatcgtggc aggaggaaaa
     5941 cgggcagcaa tgccttgtcc aattggaaat tccggcacgg attttaggca ggcaggcgga
     6001 agagtggcag tatcggcaag gtgtatatgt tcacgtcgaa ggttttttag ctcaaaaaag
     6061 cagacgttcc cttatgccga tgctcaggat acaaaatatt caagaatata aaggttaaac
     6121 gacaatggct cgtcaatcat tcaaacgtag aaaattctgc cgtttcacgg ctgaaaaaat
     6181 ccaagaagtc gattacaaac aagttgattt gctgaaagac tttatctctg aaaacggtaa
     6241 aatcattcct gcacgcatca caggaacgaa ggcattctac caacgccaat tggctgttgc
     6301 cgtaaaacgc gcacgcttcc tggctctgct gccttacacc gaccaacaca aataattttg
     6361 gagattgaat catgcaaatt attctgttag aaaaaatcgg cggtctgggc aacttgggcg
     6421 acatcgtaac cgttaaaaac ggctacgccc gcaactttct aattcccgca ggtaaggcaa
     6481 aacgtgcgac cgaagcgaat atgaaagagt ttgaagcacg ccgtgccgaa ttggaagcca
     6541 aacaggctga aattttggca gatgcccgag tccgtcagga aaaattggac ggtcaaaccg
     6601 ttaccgttgc tcaaaaagcc ggtgtggacg gccgcctgtt cggttccgtt accaatgccg
     6661 acattgctgc tgcaatcgtt gctgccggca tcgaagccgt gaaagcaaat gtacgtctgc
     6721 cgaacggtcc tctgaaagcc gttggcgagt acgaagtgga agtggctttg cacactgatg
     6781 ccgttgctaa aattaccgtt gccgtcgttg ccgcaaccga gtaattgaaa ttatcagatg
     6841 ccgtctgaaa ccctgccgat tgtcggaatg tggtttcaga cggtatttgt tttgttttcc
     6901 gtatattctg caaaaatcgg gatggttgtt tttatagtgg attaaattta aaccagtaca
     6961 gcgttgcctc gccttgcctt gccttgcctt gccgtgctat ctgtattgtc tgcggcttcg
     7021 tcgccttgtc ctgatttttg ttaatccact atatttgcct gtggtctttg caggaatagt
     7081 ttgttaacga aatttgatgc ataaaatgcg ccaaaaaata ttggctgtgt tttaatatag
     7141 tgttgataag cgtacctggc ttcaatatac tcaataggat agatctcttc ctaaccgaaa
     7201 aaataattgt cttttccctt gcaaataatt gaaatcaaca gattactaaa acacagccaa
     7261 aatattcaat tgacaaaaac ttttctgatt ttgagaaatc tgtgaaaatc aggggattat
     7321 ttattttgaa aatggtatcg ggcataaaat ttggctgtgt ttcaatatgg tgctgataac
     7381 atactttgtt tcaaaatgtt caataggata acttcctgat tgaccgaaaa ataatggcct
     7441 tttcccttgc aagcagttga aatcaacaga ttgtcaaaat acatcctaaa aatttaataa
     7501 cttatatata tgattgcaaa ggttgcggct tgtataccgg tcattatggc ggaaataccg
     7561 tttgggaggc agccgcatcg cgtggaatcg gcacggcagg cgatgcgccg atgccttcgg
     7621 gcgtgaaggc ggggcaatcg gcagtttgga aaaaggcaat ggaatctgtg ggcagaagaa
     7681 gttaagggta aaagtcgggc gcacggtcag gttgtctgtc cttttgtcgg aacaggagac
     7741 tgggcagctt tgtcctgatt aattgcttgt gcaaacggta aaaaggtttt ttcgtgtgtt
     7801 ttaaagcagg ttgtacagca tttttccggc aatcaaaagc aacataatgc cgaaggattt
     7861 tttgagtttg gcagaagaaa gtttgtgggc ggttttgaca ccgagcgggg caaaggcaat
     7921 ggttgccgcg ctgaggacgg cgacggcggg caggtaaagg aagcccagtg acccttcggg
     7981 caatcctgca atattcaggc cgttgagcag atacgatatt gcgccggaga gtgcaatcgg
     8041 ccaggcaagg ccggatgatg tgccgatggc tttatgggcg gggaagccgc agtggattaa
     8101 gaaggggacg gaaagtgaac cgccgcctat gccgacccag ctcgacattg cgccgaacag
     8161 tgtggaaacc gcagtcagtc cgggcagtcc gggcagcggg cgggatgccg tctgagggtc
     8221 ggtatgcagt gttttgaatg cgacggcggt taaaaacagg atgaagaaaa tttgaagccc
     8281 gaacgcgggg atatattttg cggagagtgc gcccgtgaat acgccgaata tcatacccgg
     8341 catcatcgca aatacggttt tccagtcgac cgcctgtttt ttgtgctgcc ccaacatact
     8401 ggaaaaggcg gtgaagacca tgacggcgaa ggatgtgccg acggcgaggt gttgcgcgta
     8461 aggatgttgt gccaaaccct gcaaatcaag tacccataaa acgacaggga caatcagcgt
     8521 gccgccgcct acgccgaaca gaccggcaat aaaacctgcc gcactgccta cggcaagcag
     8581 gattaagata atgtcccaat gccacattta atccgccttt ctgtattttt tccagaatcg
     8641 gcgtgccgcc ataatgtagc cggacaggct gtatccgaga aagaacagga agaggacgag
     8701 cgacggttcc caagtgacca gaagcagcaa taagacggca agcagcattc cgacaaaggg
     8761 gacttgtctg cggatgttga tttctttaaa actccaaaaa gggatttgga caatcatcga
     8821 caggccggca aacagtgtga tgcccaatgc ccaccagtgg acggcgggga atttttcgac
     8881 gctgtggttg acccaaatca gcccgacaat cagcgcggcg gcagtcggac tgggcacgcc
     8941 gataaaccag cgtttgtcca ccttgccgat gagcgtgttg aacagcgcaa ggcgcagggc
     9001 ggcgcaggcg cagtagatga aggcgacgga ataaccgatt ttgccgaact gccaaagctg
     9061 ccatttgtag gcaatcagag cgggagcaac gccgaagctg accatatcgg caaggctgtc
     9121 gagctgctcc ccgaacgcgc tttggctgtt ggtcagccgc gccacgcgcc cgtccatacc
     9181 gtccagcaac atagagatga ataccgcgat ggcggcggtt tcataacgtc cgtgcatgga
     9241 ttgggtgatt gcgtaaaatg cggaaaacag cgcggcgatg gtaaaggagt tgggcagcag
     9301 gtagatactg ttttgacgca gggagcgttg cggggtgttg gtcggggttt ccatagctga
     9361 agtgtctcac ggacgtggag aatcgatagg ggattataac ccaaaccatt atcgaacaat
     9421 acgcccttta ggatgaagcc ctcggaggcg gtttgttttt ctgatggcgg atgctttgcg
     9481 gggcggtttg actctgcctg ccaccgggtg tgttgtttcg gatttttgcc ggatggatat
     9541 tgttgccggg catttctggg cttgccggaa ggtatcgggg ggagggcatc ggaatatgcg
     9601 gcgcaccgtt caaaccgggg cggcgtttcc ctattttcag ggggttgcag gtttcaacaa
     9661 attgaaagat attttttgca tttccgaccg tatgctgatt ttgaagcggg ataaattata
     9721 ggacaaggtt tttcgcccgc cgcaaaggtt ggccgcaatg ccgtctgaaa acaacggctt
     9781 gccgctaagg cggcggtatg cggatttgcg ctgccgaaat cggtttgcca tacccggcgg
     9841 tgcaatctgc ggggagggat aagccgataa ggcgggactt atcctgtttt ttgtttataa
     9901 tgcaacagaa tttggacgaa ccgtcgggaa tttatcatga tgaaaaagat actggccgta
     9961 tcggcactat gcctgatgac tgcggcggca cgggctgccg atacttacgg ctatctcgcc
    10021 gtttggcaga atccgcagaa tgcggacgat gttttgcagg ttaaaactac aaaagaagat
    10081 tcgacgaaaa gcgaagcgtt tgccgagttg gaagcctttt gcaaaggtca ggacacgctt
    10141 gcgggcattg ccgaagacga gccgaccgga tgccggtcgg tcgtgtcgct gaacaatacc
    10201 tgtgtcgcgc tggcataccc gaaagccttg ggcgcgatgc gcgttgaaaa cgccgttgtg
    10261 attacttctc cgcgttttac gagcgttcat caggtcgcac tcaaccagtg catcaaaaaa
    10321 tacggcgcac agggacaatg cggcttggaa acagtgtatt gcacgtcttc ttcttattac
    10381 ggcggaactg tgcgctcttt gattcaaaat ctcaaataaa acggaaaatg ccgtctgaaa
    10441 gatgttcaga cggcatttcc atatcgacgg tcaggattct ttcggatcgg gcagcaggct
    10501 gttcaacaca atggcaagta cggcgcacaa gcccacgccg gcaaagctga agctgcccaa
    10561 tttgagcgtc atgccgccga tgcccgtggt cagtaccgag ctgacgatga ccaggttttt
    10621 cggcagcatc aaatcgactt tggcatcaat cagcgttttc acgcccaaag aagcaatcgt
    10681 gccgaacagc agcagcataa tgccacccat cacgggcatc ggaatggaag ccaaaaacgc
    10741 attgaatttg ccgaaaaacg ccatgcagac ggcaaaaacc gccgcccaag tcatgatgac
    10801 agggttactg tttttggtaa tcatcaccgc acccgttact tcgccgtagg tcgtaaccgg
    10861 cgggccgccg atcagaccgg caacgcacac gcccaaaccg tcgcccgcaa gggttttgtc
    10921 caagcccggg tctttcgtat agtctttccc cgtcacattg ccgattgcca tgatgccgcc
    10981 gatgtgttcg atggcggggg cgacggcaac gggcagcata aacagtgcag cctgccagtt
    11041 gatctgaggc gtttcaaaat ggggaacggc gaaccagggc gcgtgtgcaa tgcttgccgt
    11101 gtccaccagt cccatcagca gtgccaaaac ataacccgaa gcgacaccga tcaagatggg
    11161 aatcagcttc atcatcctgc tgccgaaaac cgatacgatg gcggtaacgg caaaggtaaa
    11221 gccggaaaga atcagcgaat cggtatagtc gatgacctgt ttgccgtccg cctgacccat
    11281 tgccatgctg cttgccgcca cggcaacaga cagaccgatg accatgatga cggggccgat
    11341 gacgaccgga ggcagcagtt tgtgtaccgc tgccagtccg cgccaacgga tcagcgcggc
    11401 aaacacaaaa tacataaagc cggcggcaaa cagtccgaac atggtggaag gcagccccca
    11461 ttcgccgacg gagtagataa tcggtgcgat aaaggcaaac gaagaaccaa gaaaaatcgg
    11521 cactttgcgt ttggttgtga tttggaacag cagcgttccc aagcctgcgc ccaaaagcgc
    11581 aagagccgga ttcagaccgg tcagcagggg aaccagcacc attgcgccga atgccacaaa
    11641 taaaatctgc gcgccggaaa cggcaagttt cagttggttc ataccatctt ctttcaatcg
    11701 gttcaaacgg cacggattat actggtttgc cgagggtctg cgtaaaaata catatggcgg
    11761 gcaacagata tgccgtctga aaggcaatgt ctgtctgttg caggatgaaa gcaggagaaa
    11821 tggagtataa tccgacactt tccatattca atccgcaagc cgtccggctt ccttatttca
    11881 gacggcattt tccggtaata cggcatgaca gaaaaatccc ataaaaaaac agcaaaaggc
    11941 agggcgggaa gcccgtcccc aacgtccgca cgcaacaaga aagccgacaa cggcgcacgg
    12001 ggcaataaag tttccgagcg gctcaaagcg gtcaaagagt tgcagaaaac cgaaaccaaa
    12061 aaggcgcgtc ccgaacatgt cgtcaacctt atcggcgacg cactgtggct gatgggtttg
    12121 gcggcaacct tgtatttggc gatttccctg atcagtttcg atatgggcga tccgtcttgg
    12181 tcgcacagtt cgccggttgt ggaagatgtc gccaattggg gcggactgtt cggcgcgtat
    12241 gttgccgatg tcggctatta tcttttcggc tggtcgttct ggtggtggat agcggctgcc
    12301 tgcgtcatgc tgtataaaaa ttttcgtctg cacgcaaaac agacggaaaa cgaggcatac
    12361 aaccacaaaa tcgctgccgc cgcgctgttt gtcctgacgg tcttcagccc cgtcttggag
    12421 tattttgtgc tgggcggaaa atatgccgac tccctgcctg tcggagcagg cggtatggtc
    12481 ggcatacgcg tcggcgcagt gtttgcgtgg ctgctgggga aatcgggcag cctgctgatt
    12541 atcttggttg ttctgctgtt gtcgttgtcc ctgctggtgc agatttcatg gctggaattt
    12601 ttgaacggtg cgggcagggc ggttcaaaac cgcctgagtg ccttatccgg caaggtcatg
    12661 gctttaggaa aacgccggcc gaatagcaaa acagacggtg tcgataccca aaatacacgg
    12721 cgcatggtaa aagaagccaa gaatattacg gccaaacccg ttgccttgcc cgaaggcagc
    12781 agcagcaacc gcaaatccgt cgcggtttcc gtcgcgccgt cgcccaaaat tcaggtttct
    12841 ctgtttgaag atgacgaacc tcggcaggcg ggcgaatacc acaagcctac attgaaccta
    12901 ttgcggattc ctgacagcga acccgtcagc atcaatcccg ccgaattgga gcgcactgcc
    12961 gaactgatcg aatccaaact ggcagaattc ggcatcggcg tacaagtcgt atccgccaca
    13021 tccggtcccg tcatcacgcg ctacgaaatc gaacccgcgc aaggtgttaa aggcagccaa
    13081 attgttgcct tgtcgaaaga tttggcacgc tctatgtcgc tgcaatccgt gcgtatcgtc
    13141 gaaaccatcg caggtaaaaa cacgatgggc atcgagttgc ccaacgacaa acgccaagac
    13201 gtgatgttga gcgaaatctt gtcctcgccc gtgtttgccg aagccaaatc caagctgacc
    13261 gtcgcgctgg gcaaagacat tgccggtacc cccgttgtcg gcgacttggc gaaaatgccg
    13321 caccttttgg tcgccggtat gactggttcg ggcaagtccg tcggcgtgaa cggcatgatt
    13381 atgtctatgc ttttcaaagc tacgcccgac gaagtccgct ttatcatgat agacccgaaa
    13441 atgcttgagt tgagcattta cgacggcatt ccgcacctgc tctgtcccgt cgtgaccgat
    13501 atgcgcgaag cggggcaggc gttgaactgg tgcgtcgccg aaatggaaaa acgctaccgc
    13561 ctgctttccc acgccggtgt acgcaatctg gaaggcttca accaaaaagt cgaagccgca
    13621 aaagcggcag gcaagccgct gctcaatccg ttcagtctga accccgacag ccccgagcct
    13681 ttggaaaaac tgccgatgat tgtggtcgtt atcgacgaac ttgccgacct gatgatgacc
    13741 gaacgcaaag ccgtcgagca gcaaatcgcc cgtctcgccc aaaaagcgcg cgccgccggt
    13801 atccatatga ttgtcgccac ccaacgtccc agtgtcgatg tcgttactgg cctgattaaa
    13861 gccaacatcc cgacgcgtat ggcgtttacc gtgcaaagca aaatcgacag ccgtaccatc
    13921 ctcgaccaaa tgggcgcgga cgaactgctc aaatatggcg attcgctgtt cctccagccc
    13981 ggcagtgccg aaccgacccg cctgcaaggc gcgtttgttt cagacgacga agtacatcaa
    14041 gtcgtcaact acgtcaaatc gcaagcccca gccgactata ttgaaggtct gctcagcggc
    14101 gaggccgcgc tggaaactgc caatatcgtt aatccgaatg cagacagcga cgaattgttc
    14161 gatcaggcag tcgcctatgt tttggaaagc aaaaaaacct ccatttcgtc tttgcagcgg
    14221 cagttgcgta tcggctataa ccgcgcggca aacctgatgg aggcactgga aaatgcgggt
    14281 gtcgtttctc ccaccgacct caacggcagc cgtaaaattt tggcgcacaa ggaccatttg
    14341 tagcccgtat tgcaaaatgc cgtctgaacg gcggaattgg cgtttcagac ggcatattat
    14401 gtttcaggcg gaacattgtg atatacttgc cagctaaaat ctcccctttg cggcaatgcg
    14461 gttcaaatat ccgtaccgtt gcgctgtttg cttccccatg cagggaagaa ggtttaccat
    14521 tttatcaaca caacaaattt aagggcttat gatgagcgta actgttgaaa ctttagaaaa
    14581 tctggaacgc aaagtagtgt tgtccctgcc ttggtccgaa atcaacgcag aaaccgataa
    14641 aaaactgaaa caaacccagc gccgtgcaaa aatcgacggt ttccgcccgg gtaaagcacc
    14701 tttaaaaatg attgcccaaa tgtacggtgc gagcgcgcaa aacgacgtga tcaacgagct
    14761 ggtgcaacgc cgcttccacg atgttgccgt tgcccaagag ttgaaagtgg caggcttccc
    14821 ccgttttgaa ggcgttgaag aacaagacga taaagaatct ttcaaagttg ccgccatttt
    14881 tgaagtgttc cccgaagtcg ttatcggcga tttgtctgca caagaagttg aaaaagtaac
    14941 cgcttccgtc ggtgatgccg aagtggacca aaccgtagaa atcctgcgca aacaacgcac
    15001 ccgcttcaac catgtcgaac gcgaagcccg aaacggcgac cgcgtcatca ttgactttga
    15061 aggcaaaatc gacggcgaac cttttgccgg cggcgcatcc aaaaactacg ccttcgtatt
    15121 gggcgcaagt caaatgctgc ctgaatttga agccggcgta gtcggcatga aggctggcga
    15181 aagtaaagac gttaccgtca acttccctga agaataccac ggcaaagacg ttgccggtaa
    15241 aactgccgtg ttcaccatta cgctgaacaa cgtttccgaa gcgactctgc ctgaagtcga
    15301 tgcagatttt gcaaaagcct tgggtattgc ggatggcgat gttgccaaaa tgcgtgaaga
    15361 agtgaagaaa aacgtaagcc gcgaagtgga acgccgcgtg aacgaacaaa ccaaagaatc
    15421 cgtaatgaac gcgctgctca aagccgtaga gttgaaagtt ccggttgctt tggtcaatga
    15481 agaagccgcc cgtttggcaa acgagatgaa acaaaacttc gtcaaccaag gcatggccga
    15541 tgccgcgaac ttggatttgc ctttggatat gttcaaagaa caagccgaac gccgcgtgtc
    15601 tttgggtctg attttggcca aactggttga tgaaaacaaa ctggaaccga ctgaggagca
    15661 aatcaaagcc gttgtcgcca acttcgcaga aagctacgaa gatcctcaag aagtaatcga
    15721 ctggtactac gcagagcctt cccgcctgca agtcccgact tctttggcga tagaaagcaa
    15781 cgtcgttgat ttcgttttgg gtaaagccaa agtaaacgaa aaagctttgt cttttgacga
    15841 agtgatgggc gcgcaagcct gattatcctg aaaatgccgt cttagggcag tttttgaaag
    15901 caccgaagcc gcttttaaag cccgctttgg tgcttttaca tcatgaaagg agacgaaatg
    15961 tcttttgata actatcttgt ccctaccgtt atcgagcaga gcggtcgcgg tgagcgtgca
    16021 ttcgatatct attcccggct tttgaaagag cgcatcgtat tcttggtcgg cccggtaacc
    16081 gacgagtccg ccaatctggt ggttgcccaa ctgttgtttt tggaaagtga gaatccggat
    16141 aaggatattt tcttctatat taactcgccg ggcggttcgg taacggctgg tatgtcgatt
    16201 tacgacacca tgaatttcat caagcccgat gtatcgactt tgtgcttggg gcaggcagca
    16261 agtatgggcg cgttcttatt gtcagcaggc gaaaaaggca aacgtttcgc cctgcccaac
    16321 agccggatta tgattcacca gcctttaatc agcggtggtt tgggtggtca ggcatccgat
    16381 attgaaattc acgcacgcga actgttgaaa atcaaagaaa aacttaaccg cctgatggcg
    16441 aaacattgcg gccgcgattt ggcagatttg gagcgcgaca ccgaccgtga taatttcatg
    16501 tccgccgaag aggctaaaga atatggtctg attgatcaaa tcttggagaa ccgcgcttct
    16561 ttgcagcttt aataagaaac ctgagagaga caaatgccgt ctgaacattt cagacggcat
    16621 ttattttata gagactgtat cgggatgact tttcctttat tgcgtttaga actgttttcg
    16681 ccataattgg ttttaacata ttcctctaca atagctagga attcgcttgc aatattatca
    16741 cctttcagtg ttattttgcg ctccccatct acataaaccg gcgcgacggg tgtttccccc
    16801 gtaccgggca ggctgatgcc gatgtcggcc aatttgcttt ctccgggccc attgacaacg
    16861 cagcccatta cggcaacgtt cagggattca accccgggat aaagggtacg ccatatagac
    16921 attttttggc gcaggtaatt ttgaacatct tgtgccagct cttgaaatac ggtactggtg
    16981 gtacgcccgc agccggggca ggcggtaacc atcggcgtaa acgaacgcaa tcccatagtc
    17041 tgtaaaatct cttgcccgac gacgacctcc tgagtacgcg ggctgccagg ttccggagtc
    17101 agtgaaatgc ggatggtgtc gccgattcct tcttgaagca agacggataa tgccgccgtt
    17161 gatgcgacaa tgcctttgct gcccataccg gcttcggtca aacccaaatg cagcggatag
    17221 gcgcaacggc tgcccagttc gcggtaaacc tgaatcaaat cctgaaccgc gctgactttg
    17281 cacgacagga tgattttgtc ttcgggcagt cccaatagaa cggctttttc ggcagattcc
    17341 aaagcggaga caatcagtgc ttccttcgtc acttcttcgg gcggtttcgg cgcggaagaa
    17401 gcgaggttgg catccatcat acgtttggcg aggctttgat ccaaagaacc ccagtttacg
    17461 ccgatgcgga cggctttatc gttttcagca gcagtccgaa tcataaaggc aaatttttca
    17521 tcgcctttta cgcctttgcc gacattgccg ggattgatgc ggtatttgga caatgctttg
    17581 ccgcattctg gaaattccgc caacaggcgt tcgccgttga agtggaaatc gccgataagc
    17641 ggtgtggcat agcccatatc gtccaagcgg cggcggattt cggcaacttt ggacgcggct
    17701 tcggggctgt tgacggtaat acgcaccatt tcggatccgg catcgctcaa ttccttaatc
    17761 tgcaatgcgg tggcttttgc atcggcagtg tcggtgttgg tcatagattg gataacgacg
    17821 ggtgcttctg aaccgacggt aatatgatcg atgcggactt gatgcgtctt gcggcgttgg
    17881 agtgtgttca tatggtttga atctgtttat tgaccggtga ggacggtttg caattcttcc
    17941 gagtagggga aattcgcctg caattgtgct tcatattcgt atgccgcctg tgcgttgccg
    18001 agggctttgg caattttcca gcctagcagc aaatcatcgg cctgaaggac ttctaccctg
    18061 ctttggtatt ttttaaagta gtaatcggca tcgcccaact gcccggccag cattttggtg
    18121 cgcgccagtt ctttaaatgc gggtgggaac tgcggctggg cggcgaggga acgtttcaaa
    18181 taggcttccg ccaatccgaa ttgcccctgt tttgcgctgc atatgccttt attcaggttg
    18241 gcaatataag gggtcgggta ggtggggtcg gccagggctt tgtcgaaata tgccatagat
    18301 tcggcagggc ggttgagcct gccgcacagg aaccagccgt agttgttgtt gatttcggca
    18361 ctgtcgggtt tgatggagag ggcttgccgg aaactttcct gcgccttgtc gttaactttc
    18421 aggtattgat agatttcggc acggaccagc caggcaagct cgtttttagg gtctgatttc
    18481 aaggcgtctt caatacttgc cgtcgcctga cggtagtcct gaccgcgcat atattccatt
    18541 gccaactggg ttttgatatt ggaaacctga ttggcttttt ctgcccgcga ggggcggtag
    18601 gaagtgctgc acgcgcccaa ggcaaggaca agtaataaag agattcgttt ggatggctta
    18661 aaaggcataa ttacccctgt tgtccgatta aaatctgctg ccatttttgt tggcggcgcg
    18721 ttttatcctg aacctgcccc gccaactgtc cgcaggcggc atcgatgtcg tcgccgcgcg
    18781 tttttcgtac ggtaacgaca aatcctgcct gctgcaaaat atcgcggaac acacggatgt
    18841 tctcattgct ggagcgttcg tatccggagt ttgggaaggg attgaacgga atcagattga
    18901 acttgcaggg aacatctgtg accagttcga tcagttcgcg cgcatgttgc gccttatcgt
    18961 ttattccgtc caacatgacg tattcgaaag tgatgaaatc cctgggtgct ttgaccagat
    19021 agcgttggca tgcggccatc aattctttca agggatattt tttgttcaac ggtacgattt
    19081 ggttgcggac ttcgtcattg gaagcgtgga gggaaaccgc caaagccacc ggcatgacat
    19141 cgcgcaacct gtccatttgg ggaatcatac ccgaagtgga aacggttacg cggcggcggc
    19201 tcaaaccgta gccgtggtcg tccagcatga tgcttaaggc ggtaacgaca ttgtcgaagt
    19261 tcgccatcgg ctcgcccatg cccatcatga cgacgttgga aatcacgcgc tcgtttttcg
    19321 gtgtaacgcc catcgctttg tttgcccacc acaattgccc gatgatttcg gcagcagtca
    19381 aattgcggtt gaagccctgc cggccggtcg aacaaaatgt acattccaaa gcgcagccga
    19441 cttgtgagga aatgcagagc gtgccgcgat ccgattcggg gatgaagacg gtttccaccc
    19501 cgttgcccgt accgacatcc aaaagccatt ttcgagtgcc gtctgaagat ttttgagaca
    19561 tcatcagctt gggaatttcg atgcctgcct gttcgttcag tttatggcgc aacgattttg
    19621 ccaaatcggt catttcgtta aaattttgcg cgccggattg gtgcatccaa cgcataacct
    19681 gtttggcacg gaaaggtttt tcgcccatat cggcaaaatg tcgggtcagc ccttgaaggt
    19741 cgtagttgag cagattggtt ttcatgtgtt tgtgtttttc ttaaatcaag gcttcaaata
    19801 aaaaattgca gggcaggcaa gtttgatttg aagccttttg caacagagtt ttcagacggc
    19861 atggatatat tttatgccgt ctgaagagga gttgtgcggt gtattgtatc aacgggggca
    19921 gatttcggtt tggctgaaaa agtaggcaat ttccaaagcg gcattttccg gagtgtccga
    19981 gccgtgtacg gcattgatgc tgaccgaagt ggcaaagtcc gcgcgtatcg tgccttcggc
    20041 ggcttcggcg ggattggtcg cccccatcag ttcgcggttt ttcagaacgg cgttttcgcc
    20101 ttccagtact tgaatcataa ccggaccgcc ggtcataaat tcaaccaatc cggcgtagaa
    20161 ggggcggtct ttatgaaccg cataaaattc ttgcgcctct ttgagggtaa gctgcttcat
    20221 tttggcggca acgattttca gaccgttctc ctcaaagcgg ctgtatattt tgccgatgac
    20281 gtttttgccg acggcatcgg gtttgatgat ggagatggta cgttcaatcg ccatgctata
    20341 tccttatttt tacttaagaa gaatcaaatc ggtattctat caaaaaaata acttatccgc
    20401 ctgaatcctt gggagatagg gtcaggtgcg gctgttccaa ataaacttcg gactgcattt
    20461 cgaccatccg gctggcggtg cgggtaaatt cttcggcaaa atcgccttcc gtatagatat
    20521 ggttgacatc taccgcgccg gtggcacaca gtttgacccg gaaatcgtag agtacgtcaa
    20581 tcagccaagt cagccgccgc gcctccgcct tttcttgcgg tgagagttgt tccaaacctg
    20641 aaataaaaac catttcataa tgttcggcca aatacagata gtcggactgt gagcgggggc
    20701 cgaagcacag tgcgcggaaa tcaaaccata tggcacggcc ggactcggct ttgtggggaa
    20761 tctcccgacc gtggatggtg ctgatgccgg ggttcaaatc ggtaatgcct gtcatttctt
    20821 tgaacagttt tgccagtttt gcctcatttt cttcattggc aggcgtaaag aaaatctcgg
    20881 cggggcggag ggtacgcagt cggtagtctt caccgccgtc aacgtttaag acggtcaggc
    20941 tggactcgat gagcgcgatt gtgggaagaa aactgctccg gttttgacct tgcgggtaga
    21001 gttcggaagg cgcgtagttt gaagtcgcca ccaaaacaac gccctcgtta agcaggtttt
    21061 ccagcagacg gcctaaaatc attgcatccg caatatcgct gacatgaaat tcgtcaaaac
    21121 ataatacgcg ggtttctttg gcaatctcgg cggcaacgga tttcaacggg ttgctttcgc
    21181 ttttcagggt tttcagcctc tggtggattt ctgccataaa ggcatgaaag tggacgcggc
    21241 gtttgcggcg gtaagggagg cagccgaaaa aagcgtccat cagaaagctt ttgccgcgtc
    21301 cgaccccgcc atagaaataa agccctttgg ggacttgcgg ggaacgcaaa ctcctgccta
    21361 aaaaacggtt tcttttgcgt ttgaacatca tcaattcggt ccaaagccga tcgaggtgtt
    21421 cgatggcggc tgcctgcgcg tcgtcgcgga tgaagttggg cagttgtgag gcagcctgat
    21481 accaggtcag cgggctgtgg ttttcaaacg gcggggcttt aaaaagttgg tctctattac
    21541 tcatatttaa ccttgttata cttcttgcgg cggagaaaga aaatgcttac cgtcggtatt
    21601 ataaatgatt tccttcaaac ttggcgtttt ccgtggaaaa gaataatgac gcgcacaagg
    21661 ggggtatgag caaaggcggc gtttcaatat ggtgcagata tgagctcaaa tgccttttat
    21721 agt
//
LOCUS       DAWFBO010000017        21633 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFBO010000017 DAWFBO010000000
VERSION     DAWFBO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.017"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(45..1226)
                     /gene="odhB"
                     /locus_tag="WGO87_000450"
     CDS             complement(45..1226)
                     /gene="odhB"
                     /locus_tag="WGO87_000450"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0391858.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1326..4154)
                     /locus_tag="WGO87_000451"
     CDS             complement(1326..4154)
                     /locus_tag="WGO87_000451"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0391859.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDTVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVVGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDVLAL"
     gene            complement(4359..5642)
                     /gene="gltA"
                     /locus_tag="WGO87_000452"
     CDS             complement(4359..5642)
                     /gene="gltA"
                     /locus_tag="WGO87_000452"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0391860.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5754..6002)
                     /locus_tag="WGO87_000453"
     CDS             complement(5754..6002)
                     /locus_tag="WGO87_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0391861.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6006..6713)
                     /locus_tag="WGO87_000454"
     CDS             complement(6006..6713)
                     /locus_tag="WGO87_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0391862.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WGO87_000455"
     CDS             complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WGO87_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0391863.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WGO87_000456"
     CDS             complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WGO87_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0391864.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFALPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WGO87_000457"
     CDS             complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WGO87_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0391865.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9591..10994)
                     /locus_tag="WGO87_000458"
     CDS             complement(9591..10994)
                     /locus_tag="WGO87_000458"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0391866.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSNNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     ADVEKWPADKRIMGSAKFIDEHTVQIDDHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEAKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11248..11985)
                     /locus_tag="WGO87_000459"
     CDS             complement(11248..11985)
                     /locus_tag="WGO87_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0391867.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12264..14540)
                     /gene="metE"
                     /locus_tag="WGO87_000460"
     CDS             complement(12264..14540)
                     /gene="metE"
                     /locus_tag="WGO87_000460"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0391868.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHANTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEADAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVESLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(14676..15554)
                     /gene="metF"
                     /locus_tag="WGO87_000461"
     CDS             complement(14676..15554)
                     /gene="metF"
                     /locus_tag="WGO87_000461"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0391869.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15755..16030
                     /locus_tag="WGO87_000462"
     CDS             15755..16030
                     /locus_tag="WGO87_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0391870.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16030..16155
                     /gene="ykgO"
                     /locus_tag="WGO87_000463"
     CDS             16030..16155
                     /gene="ykgO"
                     /locus_tag="WGO87_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0391871.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16263..16463)
                     /locus_tag="WGO87_000464"
     CDS             complement(16263..16463)
                     /locus_tag="WGO87_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391872.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALRDVAKISAWVLLG
                     AAAATLFWYLVMLYFKHIPDSY"
     gene            16623..17762
                     /locus_tag="WGO87_000465"
     CDS             16623..17762
                     /locus_tag="WGO87_000465"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0391873.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYAGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALTDYLGIGQWAAVVGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17759..18340
                     /gene="metW"
                     /locus_tag="WGO87_000466"
     CDS             17759..18340
                     /gene="metW"
                     /locus_tag="WGO87_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0391874.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTNSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18457..19302
                     /locus_tag="WGO87_000467"
     CDS             18457..19302
                     /locus_tag="WGO87_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0391875.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19352..19912)
                     /gene="efp"
                     /locus_tag="WGO87_000468"
     CDS             complement(19352..19912)
                     /gene="efp"
                     /locus_tag="WGO87_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0391876.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(19955..21103)
                     /gene="earP"
                     /locus_tag="WGO87_000469"
     CDS             complement(19955..21103)
                     /gene="earP"
                     /locus_tag="WGO87_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0391877.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWRVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGVLRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPLTLLLAGAQIIDS
                     LKQNGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRCLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21122..21400)
                     /locus_tag="WGO87_000470"
     CDS             complement(21122..21400)
                     /locus_tag="WGO87_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391878.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
BASE COUNT         5319 a         5493 c         5476 g         5345 t
ORIGIN      
        1 tcagacggcc ttttcattaa caaataaaag gccgtctgaa acgattacag atccaacaac
       61 aggcgggctg ggtcttccaa cgcgtcttta atggctacca aggtcaatac agcttcgcgg
      121 ccgtcaatga tacggtggtc gtaagacaga gccagataca tcattggacg gacaacaact
      181 tggccgtttt caaccacagc gcgctcttta gtggcgtgca tacccaaaat cgcagattga
      241 ggcggattga tgatcggggt ggacatcata gaaccgaaag taccgccgtt ggtaatactg
      301 aatgtaccgc cggtcagatc ttcgatagcg attttgccgt ctttggcttt tttcgcgtaa
      361 tcaacaattg cttgttcgat gtcggcaatg ctcatttggt cggcatcgcg caggattggc
      421 acaaccaaac cgcgtgggct gccaatcgcg ataccgatgt cgaagtagcc gtggtacaca
      481 atgtctttgc cgtcaacaga agcattcaca accgggtatt ttttcagggc tgcaacagcg
      541 gctttaacga agaaggacat aaagcccagt ttcacgccgt gttctttctc gaatttttct
      601 ttgtacttcg cacgcaagtc catgattggt ttcatgttga cttcgttgaa tgtagtcaga
      661 atggcgtttt cttgttgaga agccaagagg cgttctgcaa cacgggcacg caggcggctc
      721 atcggtacgc gttcttcagg acgtgcgccg gcaggaagtg caacggcagg agcagcggct
      781 gcggcaggtt tggctgcggc attttgtacg tcttctttca atacgcgacc gtcacggccg
      841 gaaccttgca atgcgttcac gtcaacaccg ctctctgcag ccagtttggc ggcggcaggc
      901 atagcggcgt tgttttgtgc agcagcagga gcggcggccg gggcagcttc ggcaggagcg
      961 gcggcaggtg cttcagcagc ggcagtagca gcggtatcga tacgtgccaa aacttggtcg
     1021 gcaacaacgg tttcaccgtc ttgcgctacg atttcaacca atacgccggc ttgtggagaa
     1081 ggtacttcca aaaccacttt gtccgtttcg atatcgatca ggatttcgtc acgggcaact
     1141 gcttcgccaa cttttttctt ccattccaag agcgtgcctt cagatacgct ttcagacagc
     1201 ataggtactt ttacatcaat aatcattttg tgtctccaat ggccctttca gacggcctgt
     1261 tgtgtgtttt tcattctgca tatgggcatt ccgcctgagc agcctcagac ggaatgctgc
     1321 ttggtttaca acgccaaaac gtcttcaacc aattgtttca attgagcaat gtgtttgctt
     1381 gagtagccca ctgcaggcga tgcgctgctt ggacgaccgg cataagacag tttttgctct
     1441 tcgctgataa cgtcttcgat gcggtggcgg atttggtaga acgcgccttg gtttttcggc
     1501 tcttcttgcg cccaaaccac agattttgcg ttcggatatt tcgccagttc ggctttaacc
     1561 tcatcgtatg ggaacggata cagctgctca acgcggacaa tcgcaacatc atcttccagt
     1621 ttacgttcgg ctcgaccggc ttccaagtca tagtaaacct gaccggcaca caataccacg
     1681 cgtttcacgc tgtcgttgct tgcgcgttcg gcggtatcgc cgacaaccgg acggaaagtc
     1741 gaaccttcgg tgaagttttc cagcgggctc attgcccctt tgaatcgcaa caggcgtttg
     1801 gacatgaaaa tcactagcgg tttgcggtat gaacctaaga cttgacgttg caagaggtgg
     1861 aacatttgcg acgcttcaga tggcatgatg acttgcatat tgttctcaga acacagttgc
     1921 aaccaacgtt ctacgcgtgc agaagagtgc tcagggcctt gaccgtcgta accgtgcggc
     1981 aggatggtgg tcagaccgca caagcgaccc catttggttt cgcctgaaga caggaattga
     2041 tcaatcgtca cttgcgcgcc gttggcgaaa tcgccgaatt gcgcttccca aatggtcagc
     2101 ttgtcaggtg cggagcaggc aaagccgtac tcgaacgcca tcacggcctc ttcgttcaaa
     2161 atggagtcga taaccaggaa ctcgcccatt ccttcgccca tatggcgcag aggaacataa
     2221 gtaccgtcgt cccatttttc gcgtttttga tcgtgcaata cggcgtggcg gtgcgagaac
     2281 gtaccgcggc cggagtcctc accggagata cgcacgccat gacctttggt tacgaggctg
     2341 gcgtatgcca aggtttcggc cataccccaa tctatggcct gtttgccgga tgccatggct
     2401 ttacgcgctt caatcacacg ttttgcagtc ggatgcaggg caaagccttc cggtacggcg
     2461 gtaaacttct cagtgagacg ctcaatatct gcggcaggca aaccggtttc gatgtgttcg
     2521 cgccaatctt tgccttggta tttgctccag tcgatttgcg tgcgttgaaa gttgctcaac
     2581 gttgtttgct cgacgtgttc gcctttgtcc aaagcatcac ggtaggcttg gatgtaaccg
     2641 tctgcctcgg cttgggttac cacgccttcg gcaatcagtt gctcggtgta caaagcacgc
     2701 gcaccggggt gttgcgatac ttttttgtac atcatcggtt gggtcaaggt cggatcatcg
     2761 ccctcgttgt gaccccattt acggtagcag acaacgtcaa tcacgatgtc tttatggaat
     2821 tttttgcggt aatccaaagc ggcttggatg gcaaagcaaa cacgctcagg atcatcgccg
     2881 ttcacatgga taaccggagc ggaaaccatt tttgcgatat cggtacagtg tacggttgaa
     2941 cgggtatcgc ggatatcgga agtggtaaag ccgatttgat tgttgatgac gatatgaacc
     3001 gtaccgccgg tggtataacc gcgcgtttta gacaggttga atgtcgcttg gttgacaccc
     3061 aaaccgataa atgcggagtc gccgtgaatc aatacgggca agactttatc gcggccgttt
     3121 tcgcccaaac gtttttgctt ggcgcgtgca gaaccttcca ctaccggatt gacgatttcc
     3181 aagtgtgacg ggttgaacgc caaagaaacg tgcatcggac catgtggagt ggcgatgtcg
     3241 gagctgaagc ccatgtggta tttcacgtcg ccgctaggca atttgatttc ggcacggcct
     3301 tcaaactcag cgaacaaatc tccgggtttt ttacccaaaa tgttcaccaa aacattcaga
     3361 cggccacggt gcgccatacc gatgatgact tcttccacgc cgtctttacc ggcgttttga
     3421 atcaggtagt tcaaaccggc aatcgcgctt tcgccgcctt cgacaccgaa acgtttctga
     3481 ccgacatatt tggtatgcag ataacgttcc aaagtctcgg cagcagtcat ctctttcaag
     3541 atacggcgtt tttgatcggc attgtaatgc ggtgtggaca atacgctttc aaaataattg
     3601 cgtacccagc ggcgctcttc ggtattggga atatagatat attccaatgc gatgtggccg
     3661 cagtaggttt gtttgaggtt gctgatgatt tgggacaaag gcagtttgcc gcgattggca
     3721 aaatcgccct cgcccatatt gaattgaagc gccatatcgg catcagacag accgtggaat
     3781 ttcggatcga gggcttcaat atcgcgcggg gggatacgtt tgagcggatc aagttgggct
     3841 gcacccacgc cttggatacg ataggcagaa atcagccgta aaacgctgac ttgctttttc
     3901 agcattgcct catccgcacc gcccgcaacg gcagatgcaa ttttcttttt cgccaaagta
     3961 acaaatgatt cgcgaatcgg tgtgtgtgcg acatcgacag caaccgtccc cggctgtttg
     4021 ctcaaatcgg tgaaatactg cttccatttt tcatcaaccg tatcggggtt ttccaaaaaa
     4081 gcctcgtaca attcctcaat gtagggtgcg ttcgaaccga acaggtaaga gaaattgagt
     4141 ttttcgtcca tcatggcgtg cgctcttttc tttaaaatat aaacaaaggg tagaagtccg
     4201 cgtcctgacg gctaaactga tgccgtctga aaagacattt tattctaccc tattttaaac
     4261 cggattgctt gtcttgattt catccgacta caatcagacg gattcgggaa acaatccggt
     4321 tctctgatat tgcctgtttg acaatatatt aatgtatgtt acctctcgcc tggcggcaca
     4381 taatcgcggc gttccgaacc ggtataaagc tggcgcgggc ggccgatttt cagcgaagga
     4441 tcgctaatca tctcgtgcca gtgcgaaatc cagcccacgc tgcgcgacag ggcgaagatg
     4501 acggtaaaca tttcggtcgg gatgcccagc gcggacagga cgatgccgga atagaaatcg
     4561 acgtttggat acagtttgcg ttcgataaag aacgggtctt tcagcgcaat ctgttccaat
     4621 tccatcgcca gtttgaattt cggactgtct tccaagccca attccttcaa aacttcatag
     4681 caggtttcgc gcataatgct ggcacgcgga tccatattgc ggtacacgcg gtgaccgaag
     4741 cccatcagac ggtatttgcg ttgtttcaca ccttccatgt atgcggcaac attagacaca
     4801 tcgccgattt cgtccaacat tttcaacacg gcttcgttcg caccgccgtg cgaagcaccc
     4861 cacaggcagg cgataccggc agcaatacag gcaaacggat tcgcacccga agagcctgcc
     4921 agacggacgg ttgaagttga ggcgttttgc tcgtggtcgg catgcaaaat aaagatgcgg
     4981 tcgagcgcgc gtgccaaaac gggattgggt ttgtagtctt cacacggcgt ggcgaacatc
     5041 atatgaagga agttttcgga ataagaaaga ttattcttcg gataattgaa cggcagaccg
     5101 tttgaatagc ggtagcacat tgccgcaatg gtcgggattt tagaaatcag gcggtaaatc
     5161 gcgattttgc ggtgttcggg attgctaatg tccaagctgt cttggtagaa cgcagacagt
     5221 gcgccgacca cgccgaccat catcgccatc ggatgcgcgt cgcggcggaa gccccggaag
     5281 aaccaagtca gctgttcatg caccatcgtg tggcggcgga cggtattgtc aaattctgcc
     5341 ttttgctcgg gagtcggcag ttcgccgtaa atcaacaggt agcagacttc caaataatcg
     5401 gacttttcgg ccagctgctc gatggggtat ccgcgataat aaagcaagcc ttgatcgccg
     5461 tcgatgtaag taattttaga ctcacagctt gcggttgaaa caaatccggg gtcgaaggaa
     5521 aacaaacctg tattttttgt cagcccccga atgtcaacca catcgtgccc gatgctggct
     5581 tccaataccg gcagctccaa acctgcctga cccggtacgt tgagtttgat tgatttggac
     5641 atattattgc tcctttgcaa gttataaccc acgttactgc atccgcagca cgttctttgc
     5701 cttcagacgg cttttgcatt ttgaaatctg cctgcaggca gatttcagat gcctcatgcc
     5761 cgtctgattt tttcaagcat cgggataagg tgccctttgt ccgtttccga atgcccgtta
     5821 atcaaggcaa gcaattcttg atcttgaaat tcaaggattt cggaaaactc ggacagctct
     5881 ttatcgctca aatgctcgaa ttctttttcc ataaacctgc cgaagattaa atctaactcc
     5941 aacaatcccc ggcgggtttg aaaacggatt ttccgtttgg caatatcgtc aaaaaccatc
     6001 atttcttaaa cggcccgttt caacataatc tctttaatct taccgatggc tcgggtcgga
     6061 ttcaagtgtt tgggacatac gtctacgcag ttcataatgg tgtggcaacg gaacaaacgg
     6121 tatgggtcgt tcagattatc caaacgctca ttagtgatgg tatctcggct gtccgcaatg
     6181 aagcggtaag cattcagcaa accggacgga ccaacgaatt tatcagggtt ccaccaaaat
     6241 gacgggcagg cagtcgaaca gcaggcgcac aaaatacact cgtacaaacc gtccaactct
     6301 ttacgctctt cctgagtttg cagacgctct ttgtccgcat caatcggatt atcgttgaca
     6361 acataaggtt tgacggaatg gtattgtttg aagaactggg tcatatccac aatcaggtca
     6421 cggataacag gcagacctgg cagaggacga attttaaccg gctgtttcaa gccacgcaga
     6481 tcggtcaaac acgccaagcc gtttttgccg ttgatgttca taccgtcaga accgcaaatg
     6541 ccttcgcggc aggagcggcg gaaagacaag gtatcgtctt gtgctttcag gcgtaccaaa
     6601 gcatccaaaa gtttcacgtc ggtcggttcc aattccaact cgtaacgctg catataaggc
     6661 ttggcatcaa catccgggtt gtaacggtaa atttcaaaac tcattttttc catgagaagt
     6721 gttccttttc tcatcaactg cccaatgatt ttgtattata aattcaagca gttcaatgtg
     6781 ggtatggtta gatttcagac gagcttatga ggcgaaaacc gtctgaaaac gcatcaataa
     6841 acgcgcttgg ccggtttgat gtattccacg ctcaaaggct tggtatgtac cggtttgtaa
     6901 gacaaagtat tggtatctga atggtacagc gtatgtttca tccagttttc atcatcgcgc
     6961 tcaggatggt cgtctgaagc gtgcgcaccg cgtgattctt tacgtgcttc ggcagacacc
     7021 aaagtcgctt tcgccacttc aattaggtta tccaattcca aagcctcgat acgcgcggta
     7081 ttccacactt tgctcttgtc tttgatttcg gtacgtttca cacgctcggc aatcgccatg
     7141 acttctcgaa cgcctttgct cagaatctca tcagtacgga acacgccggc gtgcagttgt
     7201 acggagcgtt gcaattcgcg acgcaatgca tcaacgtttt cgccatcagt ttgattgtcc
     7261 aaacgctcga tacgttggcg ggtcagctcg cccgcatccg caggcaaagg tttccagtcg
     7321 ctttgctctt tgatgaattt aatcatgctg tcgccggcag ctttaccgaa taccaccaag
     7381 tccaacaggg agttggtacc caagcggttc gcaccgtgta cggaagcaca agcgcactca
     7441 cctgccgcat acagaccttt tacaggcact tcgtattcgt cgccttgagg aacgacaact
     7501 tcgccatggt agttggtcgg aataccgccc atcatatagt gggtagtcgg cacaacggga
     7561 atcgggtctt taatcggatc gataccggcg aactgaatgg aaatctcgcg gatgcccggc
     7621 agtttttcca taattttttc tgcgccgata tggtcgattt tcagtaagac atggtctttg
     7681 tttttaccgc agccgcgacc ttcgtagatt tccatcgcca tcgcgcggga aacaacgtcg
     7741 cgagaagcca agtcttttac ggtcggcgca tagcgttcca taaagcgttc gccgtcggca
     7801 ttcaacagaa taccgccctc gccgcgtacg ccttcggtaa tcaacacgcc cgcacctgcc
     7861 acgccggtcg ggtggaattg ccagaattcc atgtcttcca acgggatacc tgcacgcgca
     7921 caaataccca aaccatcgcc ggtattcata taggcattgg tagaagacgc ataaatacgg
     7981 ccgccgccgc cggtagcaaa catcacagct ttagcgtgga aaatataaac ttcgccggtt
     8041 tccatttcca tggcggttac gccgacgaca tcgccgtttt catcacgaat caaatcttgt
     8101 gccgtccatt ccacaaagaa ttgcgtattg gcacggacat tttgttggta caaagtatgc
     8161 agcatcgcat gacctgtacg gtcggcaacc gcacaggcgc gttctaccgc gcgtttaccg
     8221 tgttcggcag tatggccgcc gaaaggacgc tgataaattt taccgctttc cacacggtca
     8281 aaaggcatac ccatgtgttc caactcaatt acggcttcag gcgcggcgcg gcacataaac
     8341 tcaatcgcat cttggtcgcc caaccagtcg gaacctttca cggtatcgta catgtgccag
     8401 tcccaacggt cttcctgcac attacccaga gaggcggaaa taccgccctg cgccgctacg
     8461 gtatgcgaac gggtcgggaa cactttagac aaaacggcac aattcagacc ggatttggat
     8521 aattggaggg ctgcgcgtaa acctgcacca ccaccgccga caatcacggc atcaaacttg
     8581 cgaacaggaa aacccatact tacccccaaa ttactttaat tgaatacacc aagcagccga
     8641 ccagccagac gatggtggca acctgcaaaa acaaacgcac gccgaagggt ttgatatagt
     8701 ccatccacaa atcgcggata cccacccaag cgtgcaagaa tacggcgatg aagctcactt
     8761 gggtaaatac ttttacccaa gtttgactaa aaaatgcctg ccatgccgaa tattctttag
     8821 gcagagcaaa tagaaccact aaaagtgcaa cggtataaat caacataata accgcggtcg
     8881 cacgttgcat cgcccaatcc cgcaaaccgt aatgggcacc ggtcaattta cgttctacca
     8941 taacaacgct cccaaaacga cagtcaaaac caatgcagaa gcaaatacgg ctttagcggt
     9001 attgcgcgca gtattcagct caaggccttt gtgcgcatcc aaaaataaaa agcggatacc
     9061 ggcgagagaa tggtgcagat aagcccacaa tacaccgatt aaaaccagct tgaccaaagg
     9121 atgggaaaca atggcacggt aagtttcaaa tgcagactct tgactcaggg taccggacag
     9181 gaaatacagc aggaaaggca gcataataaa cagcccgacc ccgctgatgc ggtgaaggat
     9241 ggaaactatc ccgggtatcg gcagacggat gttcggcaaa tccagataaa caggacgcgg
     9301 tttggcagac atagtcaatt cctctgtagt tatatatcga ctcaaaaacc gtaaacccat
     9361 tgctacactg ggttacataa acgttaattt acctgtttta tcccattttg aacagtgtat
     9421 tttaaaaaca aactgataga ttttgacaaa cacgcgtgga aacagcagat taaaagaaag
     9481 atttttagtt ttgccgcccc cttcccgaca cggtatcagc atccgacccc atccccaaaa
     9541 acaaaatgcc gtctgaaaaa atttcagacg gcattgtttt gccatatcgg tcaggctttc
     9601 aatttcgcat cggcatcgcg caacgcggta cgcaaacctt cctcgataac gggatggtag
     9661 aacggcatat ccagcatttg cggaacggtc atcttcattt gatgtgccca agccaacagg
     9721 tgcgccaaat gttcggcggc cggacctacg atttctgcgc cgatgaagcg gccggtggct
     9781 ttttcggcat acaggcgcat atggcctttg tttaccagca tcacgcggct gcggccttgg
     9841 tttttgaacg atacttcgcc gatgacaaat tcgtcggctt ggtattgcgc ggcaacctgc
     9901 gcgtatttca aaccgacaaa gccgatttgc ggactggtga acactacgcc gatggtgctg
     9961 cggcgcaaac cgccgccgat attcgggtag cggcctgcgt tatcgccggc aatcttgcct
    10021 tggtcggcgg cttcatgcag caggggcagt tggttggacg cgtcgcccgc aataaagata
    10081 tgcggaatac tggtctgcat agtcagcgga tcggcaacgg gtacgccgcg cgcatctttt
    10141 tcgatattga tgttttccaa accgatattg tcaacgttcg gacggcggcc tacggctgcc
    10201 agcatatatt cggcaacaaa tacgcctttt tcgccatcct gctcccaatg gacttctaca
    10261 ttgccgtctg cgtcgagttt ggcctcggtt ttagcatcca aatgcagttt caattcttca
    10321 ccgaacacgg ctttcgcctc gtctgaaacg acggggtcgg aaatgccgcc gatgatgcca
    10381 cccaaaccga aaatttcaac tttcacgccc aaacggtgca atgcctgacc cagttccaaa
    10441 ccgataacac ccggtccgaa cacggcaacg cgcttaggca gcgtatccca tgagaaaacg
    10501 tcatcgttga taatcaaacg attgcccaaa gactgccatt gcggcaggat gacgggacgc
    10561 gaaccggtag caatcacgaa actttttgcc gtaatttggg tatggtcgtc gatttggacg
    10621 gtatgctcgt cgataaactt agccgagccc atgatgcgct tgtcggcagg ccacttttcc
    10681 acatcagcaa cgacaaagcc gacaaaacgg tcgcgctcgg atttaacacg ccgcattact
    10741 tcttcaccgt tgacgacgat gctgtctttg tccaaatgca cgccgaacgg gtcggtatgc
    10801 agtgcgtggt ggcgcgcttc tgcggcggca atcaagagtt tggaaggcat acaacccacc
    10861 cgcgcgcagg tcgtgccgaa cacattgttt tcaatcaggt aaacattgtt cgaatgcaaa
    10921 cgggcattgc gaaacgcgcc cataccggca gtaccgccgc cgattacgac gacatctgct
    10981 tgaatttttt tcataatctt tgtccttctt tattaagcgt ccgaagcagc atcagcgtga
    11041 tgcgtatcag gttttcagac ggctttttta ataaactatc gacaatataa aaccaatagc
    11101 cattttaaaa gtttcgattt aaaacaataa tctcaaattc tacttgtaaa tatagagcta
    11161 ttcagaacga ccgtgtagcg tatcgtctga ataactctat tttcctgttc agacggtttt
    11221 attcaaaccg ccttaagcaa acagctatca gtttttagcc aagtaagctt ccaaatcttc
    11281 gctgccgccg atgtatttgc cgccgatgaa gacttgaggg gcagtcatct tgccggtaat
    11341 ggcgcgaacg gaagtaacgg ttgcatcttt gcccaatacg atttcttcgt aagacaaacc
    11401 tttgtcttgc aaagcttgtt tggctttagc gcagaattgg caacctggtt tagtgaaaat
    11461 tgccacagac tcttgagcct tccaatcggg agcaacgaat tgcagcatag tatctgcatc
    11521 ggatactttg aacggatcgc ccggttcttc aggttcgatg aacatttttt caaccacgcc
    11581 gtcgttaacc agcatggagt aacgccaaga gcgtttaccg aagcccaagt cttctttacc
    11641 gaccagcata cccatacctt cggtaaattc gccgttgccg tcaggaatca tgtagatgtt
    11701 gtcggattct tcttcggcag cccaagcgtt cattacgaac gtatcgttta cagatacgca
    11761 gtagattgcg tcaacgccgt tttctttgaa cgcgccgaac aattcgttgt aacgcggcag
    11821 gtgtgaagaa gaacaagtcg gggtaaatgc accgggcagg gagaatacga ctactttttt
    11881 gcctttgaac aaatcatcgg tagacacatc tttccaagtg tcgccgacgc gggtgcggaa
    11941 tactacggaa ggtacttttt gaccggtacg atcttgcaaa gccattttaa gctccttaat
    12001 agattggttt gagaaataat tgtgaatcgc tgggcattct accccatgca aaatgatttg
    12061 tataatttat agttcaaata atataaatta tttaaaacta tcaaacacaa ctccgatttg
    12121 cctgtttccc acatataaag ccgaattaga aaaaaacaag ctgcaatgaa aagattttcc
    12181 ggcaactgcg cccttgcgcc gcaaatcagg acaaaatacc gtctgaaagg ctttcagacg
    12241 gtatttattc atacggtctc ggcttatttc gccaattcgg cacgcaattt gtgggttacg
    12301 ttcatcatca cttggagttg ttccagagtt tctttccagc cgcgtgtttt caggccgcag
    12361 tccgggttaa cccacagacg ttcaaccggt acaacctcga tggctttgcg caacaggtgc
    12421 tccacttcgg cttctgtcgg tacgcgcggg ctgtggatgt cgtaaacccc cgggccgatg
    12481 tcgttcgggt atttgaattc gccgaacgcg gtcaagagtt ccatgtcgga acgtgaagtc
    12541 tcgatggtga tcacgtccgc atccattgca gcaatcgcag gcaggatatc gttgaactcg
    12601 gagtagcaca tatgagtgtg gatttgtgtg ctgtcttcgc aaccggcaga ggacaggcgg
    12661 aaggattcgc ccgcccagtt caggtaggca tcccaatcgg cgcgtttcag cggcaagcct
    12721 tcgcggatgg caggttcgtc aatttggatg actttgatgc ctgctttttc cagatccaat
    12781 acttcgtcgt tcagagccag tgcgatttgt ttgcacacgg tagagcgagg aatatcgttg
    12841 cggacaaaag accattgcag aatggttaca gggccggtca acataccttt catcgggcgt
    12901 ttggtcaggc tttgtgcgta agtagaccag gcgacagtca tggcttcagg acggcttacg
    12961 tcaccgaaga taatcggtgg tttaacacaa cgtgagccgt agctttgtac ccagccgtat
    13021 tgggtgaatg caaaaccgct caacaattcg ccgaagtatt cgaccatgtc gttacgctcg
    13081 gcttcgccgt gtaccagtac gtccaagtcc agtttttctt gctcttcaac taccaaggcg
    13141 atttcttttt tcatcgcggc ttcgtaatcg gcagcagaca gttcgccttt tttgaaggct
    13201 gcacgtgctt ggcggatttc ggtagtttgc gggaaagaac cgatgttggt agtcggcagc
    13261 agaggcaggt tcaaccatgc ttgttgcgct ttgatacggt cggcaaatgg agatttgcgt
    13321 tggtctgcgt tggcaggcaa atcggccagg cgtttggcaa cgtctgcacg atggatttcg
    13381 ctgctgttgg cacgggagtc ggcagcagct tggctggcgg ccagttcttc ggcaacagaa
    13441 tcacggcctt cgttcaatgc ggctttcaga acgcgcaatt cttgggtttt ttgcagggtg
    13501 aatgccaacc aagagtacag gtcaggtttg ttggctttca gtttttcttc aactgacaag
    13561 tcaaatggag tgtgcagcag agagcaagag ctggagatcc acaaacggtc gcccagtttg
    13621 gcttgcagag actcaacagt ttccaaaact ttgttcaggt tggcgcgcca aatattgcgg
    13681 ccgtcaatca cgccggcgga caggactttg tcgtagtcgg cgaacgcgtc cagttgctcg
    13741 ggagcgcgta ccaagtcgat gtgcagaccg tcaacaggca gggctttcag caatgcggcg
    13801 tgttcggcaa cagaaccgaa gtaagtgctc aacaggattt tggcacttac tttgttcaaa
    13861 gtagcgtaaa cgtctttgta ggcttccacc cattctttag gcaaatcgac agccaaagca
    13921 ggctcgtcga tttgaatcca ctcggcatcg gcttcaacca aagcagtcag gatttcaacg
    13981 taaacaggca acagtttagg caacaggctc agacggtcga attcgacggc gcctttttct
    14041 ttacccaccc acaggaaagt caacggacct acaacggtcg gtttggcttt cagaccgagg
    14101 gcttgggctt cttgcagttg ttggacgtag tgtttggcgt tggctttgaa ttcagtattg
    14161 gcgtggaatt caggcaccaa gtagtggtag ttggtgtcga accatttggt catttcgata
    14221 gcgaattggt ctttgttacc gcgcgccagt tggaagaatt gttccaaaga caggttttgg
    14281 ctgtcgaagc cgaagcgggc gggaatcgcg ccggtggcga cttgcaggtc gaggatgtgg
    14341 tcgtagaaag tgaaatcgcc tgcggcaacg taatcggcgt tggcagcagc ttggtgtttc
    14401 cagtttttct cgcgcaagtc tttagcaaca gccagcaatt cttgctcgct gatttctttg
    14461 cgccagtatt tttcttgggc gaatttcaat tcgcggaagg cgccgacacg cgggaagcct
    14521 gagaaatgta atgttgtcat gttgaactct ccagttggat tttgtaagaa ccggttcaat
    14581 ccgaatcggt cggtattgta aatcagaatg gctcggatgg ggcggcgttt tgcttttcag
    14641 acggcctcgg atgccgtctg aagccgtttt cagctttaag ggcgcacgcc taaaatatgg
    14701 cagatggcgt aagtcagctc gctgcggttg agcgtgtaga agtggaaatc tttcacgcct
    14761 tcgcgggata ggactttgac catatcgatg gcgatactgg cggcgacgag gttgcgcgtg
    14821 ccttggtcgt cgtccaaacc ttcatacatt tgcgacagcc agcttgggat tttgacgttg
    14881 gtaacctgcg ccattttgcc gagctgcttg aaattggtaa cgggcaggat gccggggacg
    14941 atttccacat cgatgcccaa catcacgcag cggtcgcgga agcgcaggta gcgttccaca
    15001 tcaaagaaaa attgggtgat gacgtggttc gcgcccgcat cgattttgcg tttcaaatta
    15061 atcaggtcgg cttgtgcgga tttcgcttcg ggatgcactt cgggatatgc tgctacagag
    15121 atgtcgaagt cggcgacgga gcgtaatagc ttaaccaagt cttcggcata aaacggtttt
    15181 ttctcgtaac cgggcggctc gtcgccgcgc agggcgacaa tgcggcggat gccgctgtcc
    15241 caataatctt tggcaatttg gcgcaattcg tcgggagaag cgtcgatacc ggtcaggtgc
    15301 ggcgcggctt ccaagccggt ttcttgctta atgcgtttga cgatgccgtg cgtgcggtcg
    15361 cgttcgccgg aatttgcgcc gtaggttacg gatacgaact tgggatgcag ggtttgcaga
    15421 cggtggatgg aatcccacag catcgtttcc atttgttcgt ttttcggcgg gaaaaattca
    15481 aacgaaacgt tgatgtcgcc tttcaaatcg gaaaggctgt tgtttaacgc attgatttct
    15541 tttgcgtaat tcatggctgc gtacctttta ttatctttta tatgatattt tcaggcaaca
    15601 taataaagtt gcgccaaaca tgagtcaatt tcaaaatttt cagatttatc atgaaaattt
    15661 ctaatatatt acaaaaatag ttaaatttcc ttgtacaagc cttctaattt atgataaagt
    15721 ttgttatgtt ataacaattc aactaaggcg atatatgaaa ccgaatattc atcctgacaa
    15781 ttatcgaacc gtcctttttt tcgacagcag cgccaatgaa ggctggttga ttcgttcttg
    15841 cgccgaaaca cacggaaaaa cgatggtttg gacagacggc aaagaatatc cgctgttttc
    15901 gctggatact tcttccgcat cccatcccgt ctatactggc aaacagcgca acgtcaacac
    15961 cgaaggccgt gccagcaaat tcaaccaacg tttccaatcc gtcatgtcat cgtttagaaa
    16021 ggacaaataa tgcaggttct gtcttcactc aaaaccgcca aacaacgcca ccgcgactgc
    16081 caaatcgtca ggcgcagggg caaagtttac gtcatctgca aaagcaatcc gcgttttaaa
    16141 gcacgccaac gctgactccc tgcccttcct gcttcggttg taaatcccct cctaccgcaa
    16201 gcccttttca tcaaatatgc cgtctgaaac agaaaaatgc gtttcaggcg gcattttttc
    16261 cgtcaatacg aatctggaat gtgtttgaaa tacagcatga ccaaatacca aaacagcgtc
    16321 gcggcggctg caccgagcag cacccacgcg ctgattttgg cgacatcgcg cagggcgcgt
    16381 ttctgcctgc ggctgaagag gcggttgata atcagcgcgg caagtgcgaa aaagaaaaaa
    16441 atacggaaaa gtctgcctat catatcggga agcggggtta gggctgtctg aaaaagtatg
    16501 gatcggttgc ggcggcatag ggacggatgt ttcatcgcat cgggctgctt gtccgaccgt
    16561 gtcaaaaagt catgccgttt gctatataat aacgcttttc gccgcatttg ttggaacagg
    16621 atatgagtca aaatgcctcg gtgggcattg taacgcccca aaaaattccg tttgagatgc
    16681 cgctggtttt ggaaaacggt aaaactttgc cgcgtttcga tctgatgatt gaaacctacg
    16741 gcgagctgaa tgccgaaaaa aacaatgcgg ttttaatctg ccacgcgctg tcgggcaacc
    16801 atcatgttgc gggcaggcat tcggcggagg ataaatatgc cggctggtgg gacaatatgg
    16861 tcggtcccgg caaaccgatt gatacggaac gttttttcgt ggtcggtttg aacaatctgg
    16921 gcggctgcga cggcagcagc ggacctttgt cgatcaatcc tgaaacgggc agggaatacg
    16981 gcgcggattt tccggtggtt acggtgaagg actgggtaaa atcccaagcc gcgcttaccg
    17041 attatctcgg catcgggcaa tgggcggcgg ttgtcggcgg cagcttgggc ggtatgcagg
    17101 ctttgcagtg gacgatttcc tatcccgagc gcgtgcgcca tgccttagtg attgcgtccg
    17161 cgccgaaact gtccacgcaa aatatcgcgt ttaatgatgt agcacgtcag gcgattttga
    17221 ccgatcccga tttcaacgaa ggacattacc gcagccgcaa caccgttccc gctcggggct
    17281 tgcggattgc ccgcatgatg gggcacatca cctatcttgc cgaagacggt ttgggcaaaa
    17341 aattcggacg cgatttgcgt tccaacggct atcaatacgg ctatggcgtt gaatttgaag
    17401 tagaatccta tctgcgctat caaggcgaca aattcgtcgg gcggtttgat gccaacacct
    17461 atttgctgat gaccaaggct ttggactatt tcgatccggc ggcggatttc ggcaacagcc
    17521 tgacccgcgc cctgcaaaat gtgaaagcga aattctttgt cgccagcttc agcaccgact
    17581 ggcgtttcgc acccgaacgt tcgcacgaac tggtcaaggc actgattgcc gcccaaaaat
    17641 ccgtgcagta tatcgaagtc aaatccgcac acgggcacga tgccttttta atggaagacg
    17701 aagcctatat gcgtgcggtc gccgcctata tgaacaacgt ttataaggaa tgtcagcaat
    17761 gaacctgcgc gatgatttgc aactgattta cgaccggata cccgaaggca gccgcgtctt
    17821 ggacttgggc tgcggcgacg gcgaactgct tgccgcattg gtcgaacaca aaaaatgcag
    17881 cggctacggc atcgaaatcg acacaaatag cgtcatcgcc gcgatgtcgc gcggcgtaaa
    17941 tgttatccaa gccgatttgg aagaaggttt aaccgcattc aacgatcaaa gttttgatgt
    18001 gattgtgttg agccaaacca tccaagcgat gcagaatacc gaaaaaatcc tgcgctgcct
    18061 gatgcgcgtg gcaaaacagg caatcgtcag cttcccgaat ttcggctact ggcgcaaccg
    18121 cgtccaaatc gcactcggcg gacatatgcc ggtttccgaa cgtatgccct accattggta
    18181 tgatacgccg aacatccatt ggtgtacgct caaagacttt gatttgttgt gcgccaaaaa
    18241 caacatacgc gtcctcgagc gcgccgtgat gacgggcaac agacaggtca aacatttccc
    18301 caaccttttg ggcagccttg cgttttaccg cgtcgggtaa gacagattga gaaccttgtc
    18361 ggcaataaca ggttcttcgg ttctattaac aacaggccgt ctgaaaaatg aaatggcagg
    18421 ttttcagacg gcctctttaa tattggaaac aatagaatga aaaacaaaac ctcatcactt
    18481 ctcttatggc ttgccgcaat gatgctgacc gcgtgttccc cgagcaaaga agataaaacg
    18541 aaagaaaacg gcgcatccgc cgcctcgtcc acggcatccg ccgcttcgtc ttccgcgccc
    18601 caaaccgatt tgcaaccggc cgcatccgcc cctgataacg tcaagcaggc agaaagcgcg
    18661 ccgccgtcaa attgcaccga cctgcacccc gccaccggca ttgacgatct catgcagcaa
    18721 atcgccgaac acatcgactc ggactgtctg tttgcccttt cccatcacga actggaaacc
    18781 cgtttcggct tacccggcgg cggctatgac aacatacagc ggctgctgtt tcccgacatc
    18841 cgccctgaag atcccgacta ccatcagaaa atcatactgg caattgaaga cttgcgttac
    18901 ggaaagcgca cgatcagccg gcaggcacaa gatgccttga tggaacagga acgccgcctc
    18961 cgagaagcga cgctgttgct gatacagggc agtcaagaaa cccgcggaca aggcgaggag
    19021 ccgaaacgca cgcgttattt tgaagtttcg gcaacccctg cctattcgag ccggcacaac
    19081 aacggacttg gcggcaattt ccaatacatc ggccaattgc ccggctatct gaaaatacac
    19141 ggagaaatgc ttgaaaacca atcactcttc cggctgtcca accgtgaacg caatcccgac
    19201 aaaccgtttt tagacatcca ttttgacgaa aatggcaaaa tcacgcgtat tgtcgtttac
    19261 gaaaaaaaca tctacttcaa tccaaacttg gggcgaagat aaaaaaatgc cgtctgaaac
    19321 gttttcagac ggcatttttc aatgcaacaa atcaagcgcg tttgcggaat tcgccggtgc
    19381 gggtatcgat ttcgattttg tcgccgtttt cgatgtaaga catcacttgg atttcggtgc
    19441 ccccgaccag gcgcgcggtt ttcatcactt tgcctgaagt atcgcctttg acggcaggct
    19501 cggtatactc gacttcgcgt acgatgatgg tgggcagttc tacggagatg gggttgcctt
    19561 cgtagaaggt aacttcgcat tggtcttcca taccgtcaac gatgaatttc aacgcgtcgc
    19621 cgatgttgtc ggcttcgatt tcgtattggt tgaattcttc gtccataaag acgtacatcg
    19681 gatcggcaaa gtagctgtac gtacagtttt tgcgggacag gatgaccacg tcgaatttgt
    19741 cgtcggcttt gtaaatggtt tcggaagccg cgccggtcag caggtttttc agtttcatgc
    19801 tgactttggc ggaagagcgg ccgcctttga tgtattcggt tttttgaacg accataggat
    19861 cgttgccgac cataaataca ttgccggcgc gcagttcttg tgctgttttc attgtgattt
    19921 tccaatcaaa tcggttgtcg taaaacgcac tattctagcg tattttttga tgctttgaaa
    19981 caaaggcggc gagtttttcg gatgcggaag gctgcccgaa aagataacgg ctccaatcct
    20041 ccgcgccttg ccgccagccg ttttgatgtt gttgcaggat ttgccaacat tcgaggcgtt
    20101 gcgtggcgga taaagcccct ccgccgttga ggtcgtctga aaggcagcgg tgtgccgatg
    20161 cggtttcggg cgtgtagaaa ccgtgtgcct tatcccaaaa ggcgtggagt ttgtcgagat
    20221 ggacattctc gtcttgcgga tagatgtgcc aaaagaaggg ttttccggca agctgggcgc
    20281 gcacgaaact gtcttcgccg cggatgacgg cgcagtcggc aaggtgcagc agtttgtcga
    20341 agtcctgttg cggcacgaaa gggattttga cgaggcggac ggatgccgtc tgaaaaacat
    20401 cgccgtcgtt ttgcagggca tcttgcggaa taacgccgtt ttgtttgagg ctgtcgataa
    20461 tttgcgcccc cgccagcaac agtgtcaacg gactgcctgc ctgtcgccac atttccagcc
    20521 actttgccca aacatcgctc cgatagccga aaagcagcca ttcgggggcg tttttttcgg
    20581 gaagcatcag cctcttgcgc aagactccgc tatcgaaacg gacggcttcg cagtaatcac
    20641 gttcgcgtat caacccgccg cttttttcgc tgaaacccat aaaccaaaaa tatttttgaa
    20701 caccctcctg cggcgaaggc atcagatgca gcctttcatt gctttcctcc gcgctcaaat
    20761 attcccaatt cagccaaagc ggcttgtgtc ggcggataat gtgcagcaca ttttcgggca
    20821 ggtcgcaggc aaaagtttcg atgacgacat cgggaacagg cgcggtatca atatctgccg
    20881 catcggaatg ccaagtgcgg acatgaatat cctgatgaac gcagggaaca tcgggcaaat
    20941 cagggcaaag cgcacgcaag gcggacacat cgtccgtcca caaatgcacc cgccaaccga
    21001 gttcgcggtg caaaacacgg gcgagccgcc acgaaacgcc gatgtcgccg aaattgtcga
    21061 tgaccttgca aaaaatccaa cagacaaaag gaggagtatt cataggaata acggaaacaa
    21121 gctaaaacgc gccgaaatag tggaacaaat acacaaccag caaaacaatc ggaataaaca
    21181 gcaacatggt ttgacgtttc aaatcaaact cttgtttcaa atgatgcttg atggtgtcga
    21241 aaaggacgaa tttgaacaaa aacgtcgctt cgcaggcaag gatgatgccg ttgatcagat
    21301 gctggggttt gtcttcgggc ggattggcga cgaaaacggc aagcgtcgcc aaagggatga
    21361 ggcagacgac aagccagcgg acaagcaggt ttcgggacat tgcaaggctt tcaaaaaagc
    21421 aaaacgccat tatcgcccca agcgcacgcc ttgtctaacg gacgcgccgg cgatgccgtc
    21481 tgaagccttg ccgcaggaaa acaccgcacc gccgaacctt taaatacgcc cgagacgggc
    21541 gttccccgaa aaatcggacg ctgccgccga tgcctgcgga caaggctgca tcgggcgcgg
    21601 gcatattgaa aacccggcgc gtcggtttga aat
//
LOCUS       DAWFBO010000018        21602 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFBO010000018 DAWFBO010000000
VERSION     DAWFBO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.018"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(324..4493)
                     /locus_tag="WGO87_000471"
     CDS             complement(324..4493)
                     /locus_tag="WGO87_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0391879.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMHRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTGKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASATNWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4548..6395)
                     /locus_tag="WGO87_000472"
     CDS             complement(4548..6395)
                     /locus_tag="WGO87_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0391880.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAS
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6925..8154
                     /gene="sstT"
                     /locus_tag="WGO87_000473"
     CDS             6925..8154
                     /gene="sstT"
                     /locus_tag="WGO87_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0391881.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIILYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8266..9546
                     /locus_tag="WGO87_000474"
     CDS             8266..9546
                     /locus_tag="WGO87_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0391882.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNAGNTAAQGTNQAENNQVGGSQNPAPSSNPNATNGGNFGRVDLANGVLIDGPSQNI
                     TLTHCKSDSCNGDNLLSEEAPSKSEFEQLSDEDKIKKYKKDGEKFTGLVADRLQMKGT
                     NQYIIFYKPKTTSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLSGGSYALSVQGKPAKGEMLAGTAVYNGEVLHFHTENGRSYP
                     TRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGNWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9622..9837)
                     /locus_tag="WGO87_000475"
     CDS             complement(9622..9837)
                     /locus_tag="WGO87_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391883.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(9830..10045)
                     /locus_tag="WGO87_000476"
     CDS             complement(9830..10045)
                     /locus_tag="WGO87_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0391884.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10120..11463)
                     /gene="argG"
                     /locus_tag="WGO87_000477"
     CDS             complement(10120..11463)
                     /gene="argG"
                     /locus_tag="WGO87_000477"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0391885.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRTQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKK"
     gene            11790..11930
                     /locus_tag="WGO87_000478"
     CDS             11790..11930
                     /locus_tag="WGO87_000478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391886.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            11927..12727
                     /locus_tag="WGO87_000479"
     CDS             11927..12727
                     /locus_tag="WGO87_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0391887.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12767..13753
                     /locus_tag="WGO87_000480"
     CDS             12767..13753
                     /locus_tag="WGO87_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0391888.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14410..14672
                     /locus_tag="WGO87_000481"
                     /pseudo
     CDS             14410..14672
                     /locus_tag="WGO87_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14710..15084)
                     /locus_tag="WGO87_000482"
                     /pseudo
     CDS             complement(14710..15084)
                     /locus_tag="WGO87_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15257..15613)
                     /locus_tag="WGO87_000483"
     CDS             complement(15257..15613)
                     /locus_tag="WGO87_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0391889.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     ILEYAIKNEKTVIFHF"
     gene            complement(15756..16100)
                     /locus_tag="WGO87_000484"
     CDS             complement(15756..16100)
                     /locus_tag="WGO87_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391890.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(16259..16659)
                     /locus_tag="WGO87_000485"
                     /pseudo
     CDS             complement(16259..16659)
                     /locus_tag="WGO87_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16663..17403)
                     /locus_tag="WGO87_000486"
                     /pseudo
     CDS             complement(16663..17403)
                     /locus_tag="WGO87_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(17473..17790)
                     /locus_tag="WGO87_000487"
     CDS             complement(17473..17790)
                     /locus_tag="WGO87_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391891.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(17787..18398)
                     /locus_tag="WGO87_000488"
                     /pseudo
     CDS             complement(17787..18398)
                     /locus_tag="WGO87_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18482..18784)
                     /locus_tag="WGO87_000489"
     CDS             complement(18482..18784)
                     /locus_tag="WGO87_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391892.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(18963..19169)
                     /locus_tag="WGO87_000490"
     CDS             complement(18963..19169)
                     /locus_tag="WGO87_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391893.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(19135..19884)
                     /gene="mafB"
                     /locus_tag="WGO87_000491"
                     /pseudo
     CDS             complement(19135..19884)
                     /gene="mafB"
                     /locus_tag="WGO87_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(20310..20654)
                     /locus_tag="WGO87_000492"
     CDS             complement(20310..20654)
                     /locus_tag="WGO87_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391894.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(20692..21036)
                     /locus_tag="WGO87_000493"
     CDS             complement(20692..21036)
                     /locus_tag="WGO87_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391895.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(21039..21227)
                     /locus_tag="WGO87_000494"
     CDS             complement(21039..21227)
                     /locus_tag="WGO87_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0391896.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(21297..21602)
                     /locus_tag="WGO87_000495"
                     /pseudo
     CDS             complement(21297..21602)
                     /locus_tag="WGO87_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT         5128 a         5054 c         5429 g         5991 t
ORIGIN      
        1 aagtggcggg aatgacggtt cgggcattcc ttaaattacc tgtgtatcgc tgtaaatctt
       61 agagatggcg gaatatagcg gattaacaaa aaccggtacg gcgttgcctc gccttggctc
      121 aaagagaacg attctctaag gtgctcaagc accgagtgaa tcggttccgt actatttgta
      181 ctgtctgcgg cttcgtcgcc ttatcctgat ttttgttaat tcactatatc gattctgttc
      241 ccttgcaggc ggacggcggc ggcggacggt ggaaaagcag gttttcaaat gtccggtttg
      301 gcggcgcgcc gtctgaaaac cgcttatttt cctttgctgt ttccggcgga gtcttttttg
      361 tccgaaccgg agaagcggtc gaaacgtatg gtgtatgtca gctcgccgcc cgacgaacgg
      421 ctgccgatac gggcaaccgc ctgtatggcg cgggtcagcc ggtaaatcag tttgacggac
      481 tgttccgcgc tggagatgct gtattcgtag ccgatgtaga gtttgccggt cagttgtttg
      541 ccgacggtca gcacctgttc ggcggggttg agttcgccgg tttgcgcgtt gcggctgcgc
      601 ttgctggtaa agcccaaatc atccaccagc ccgatgcggt cgttgatttg cccggcaagc
      661 agcgcgcctg cggctgcgga cagggcggca ttgtcgccgc tgctgccgct gccggcgcgg
      721 ttgaggatga gccaagagag cttgtctttt tcactcatcg gttcgtttgc cgtcagcgta
      781 atgcgcgggc tgttgaggct gcccaatatt tccacgcccg caccgacggg ggaaaggcgg
      841 cgttcggcgc ggatgttgag gttggggtcg ttgagcgggc cgacaaagga gaccgtgcct
      901 ttggtaatgt ccaaatcctg cccgtatgcc ttataacgcc ctttgatgac gcggaccgtg
      961 cccacgcccc gcacgcttcc gcccgattgg gcggtcaggg tcagtttgcc gcctatggta
     1021 acgtccgcgc cgtagccggc gaagcggatg ccgtcattga ggtctaaagt caggttcata
     1081 ttgacgggga gcggtgccgc cgcctctttt ttgacttcgc ctaatacgac gacatcgtcg
     1141 ccgacggacg gcatcgagga tttttgcgaa ccgaacagcc cctgatccgt tttaatcatc
     1201 ccggtaacgg atatgccttt ttgcggcgaa tagcgcaggc gggtgttgcc ggaaaccgtc
     1261 aggcggcggt tggggcggga caggatgcgg tatttgtcga acaccgcgcc gatatcgaca
     1321 tcgggtccgc tgttttccat accgaccgta ccggagagtt ccgccgtccc ttcgtgacgg
     1381 aatttcaggc tgtcgattac ccatttcctg cctgcgatat gcgaacgcag cgaaccgttg
     1441 tccaagatga tgccttgggt ttggttgcgg taatagagct tgtcgccgtt gatgctgccg
     1501 cccaagtgcg ggtcggcgat gctgccgccg agggttacgg cggcattcag gctgcctttg
     1561 acggtttgtc cgacgggcag gaagttgcgg aatacttcgg catcggcaac ggtcaggttg
     1621 agtttcccgc ccaggggcga ggtactgaaa gaattacttt gcccgacggt gatgttgccg
     1681 ttgattttcc cgtagttgct gctgccgttg acggcggcat tgacggacgg agagccgacc
     1741 cgtccgccga tttgcgcggc ggcattcagg ctgccggtaa tgttttgcgc ggcggcgggc
     1801 agaaagggct tcaatgcgcc caagtcggga agggaggcgg taatcctgcc gccgagcggt
     1861 gcatttgcca tattgccgcc gaaggcgttg ccgatgtcca aatcggcgtt aatccgcccg
     1921 aaacgcgcgc cgccgtcaag caggattccg atgcggtcgt tttgaaagcg cgttttcagg
     1981 gaaaatgcgt tcaaacccaa agcctgcccg ccgggcaata cggcatcgcc gctttgccgg
     2041 ctgatattga ggtagccgcg cgcgttgcgc ccgtaggcga catcccagtc gccgtttaaa
     2101 accagattgt gttcgaaggg cggtttgaag aaattgtgca actcggcgat atgcagaccg
     2161 tgtgcgccgc cttttgccga tatgccggtt tttttatccc aagaaaagtg ttgcaggttg
     2221 aggctgccgc ccattgcctg ccaatttgtc gcacttgccg ccacgcgttc cgcaccggct
     2281 tcgagcgtca tacggttttg cagcttgagg ttgaatgcgc cgccgatgtc gaggatgccg
     2341 atgctgcctt tccatcgggt aagttccctg ttgatgccgc ctgaagcgtc caaatcgaat
     2401 ttgaacggtt tgccatccag cgtcatggcg gcgtgtgtgc ggatgcggtg ctgcacgccc
     2461 gtgccgtcca gcatcaggtc ggcggtatcg acaaccgccg ctccgcccga cagcgaaagg
     2521 cggctgcctt tgatgtcggc gcgtatcggg cggcttgtgt cgggcgaacc tttgagcgtg
     2581 aaatcgagcg aacggatgtc tgccgccttg ccgatgtgca ggttgcgcgc cgcgccggaa
     2641 aggtcggttt caaaggttcg gatgccgccg tccaaatcgc cggaaaggtg tccgcgtaca
     2701 tttaaagacc ccgcgagtcc gaaaccgaaa cgggataaat cgggtgcggt gatattgagg
     2761 ttaagccggt cgcctttttt gccgaagccg ccgtctgttt tgatgatgtt ccgccccagc
     2821 cgcaaatcga cggcggcacg cggaaggtgg cgggactcgt aaacaatgtc ggcactgccg
     2881 gcaatcggta cgccgttgaa cgtgccgggt aaaaaccgca ttttgcctgt gaatttctct
     2941 tttgccagtt cgccggcaag gtttattgag ccgttgatat tgcctgccgg aagttgcgga
     3001 tcgatgcgcg aagggtcgaa tgcgcgggaa cggatgtcca gcttgagcag gcggtcttta
     3061 aacagctcga gatagccttg cgcggtcagg ctgccttgcc cggcggcgat gttgacggtg
     3121 tcgagcacca gtttccgctg tccgtttgcg gggtcgcttg caatggcgag gctgccgtcc
     3181 gtgcgcgccg tgccgatgcc gagttgccaa gagattttgg gcgaggcggt cgtgccaccg
     3241 atgccgatgc tgccgtccaa cctgcctttg aacgcggttt gcagtacgtc ttccgcgccg
     3301 acggagttga tgcctatatt taaatcgagg atgtcttttc cggtgtcgat tttgcccgac
     3361 agcctgatgc cgccccgtcc gagcagggcg gcggacgtat tgccgatatg caccgtgccg
     3421 tcctgccgga tgacaaagct gcctaaaacc tgacggacgg ggatgccgtt gcggtcggca
     3481 aaaccggctt tggtgttttc caaatcgagc gagccttcca gcgcgatgcc gtctgaaaac
     3541 gacgggatgg cggtcaggtc gaaattcagc ccggcatcgg gcagggaagg tacgaaggcg
     3601 gccggattga tgttgaaccc tttgaccagt acttcttcca atgttttatc caatgattcg
     3661 gcaaacgggt ggatgacgga ttttcccgag aggcggatat tgccgccgtc gatcgccagt
     3721 tcggcgcgca catccttcag gctgccgctc agccgagccg tactgtgtat ggttttgcct
     3781 tcgagtccgc ctttggtgta aatggcggta tcgagggcaa acggtttttt caagccgacc
     3841 gaggccgccc ccgacgaact gctccacggc gtgtcggcgg ccttcaggtc aaggcggtgt
     3901 cctttgcggt cgtaacggta tgaagcatcc agccgttcga gatagacggt ttgtttgtca
     3961 aaggctttgc ccatgctgat tttgcccgtc tcgaagcggt cgagatagac ggcggcaggc
     4021 aggtctatgc tgtcgggaag gctgagcggc gggcgttctt ctttaggcgg agtcggtttg
     4081 gtaacgatgg cgatgtcgcc ggcggaaatt tcggtaatgt gcaggctgcg gtgcatcagt
     4141 tcggacggtt tccacgcgaa gcggaagcgg ctgattttaa ggtctgcccc ctcggtttct
     4201 atcgaccaat tgtcgccgtc gaagccgtcg agcagcgtgc ctttgaggtt ttgggaggaa
     4261 atgtttacgc cgaaccaaga cgggatttgg tacagcccga agcgcaaacc cgcttccgtg
     4321 ccggcgagcc agccgaggaa acatactgcc aaaatcagaa cagacagcag tgccgccgac
     4381 agcttcagca gcgggcggcg tttttttgcc ggcggggtag ggcggtgttc agacggcatt
     4441 ttgcgcgtgc cgttttcggt cggatcggta tctgtcggtg cggtatcggt cataatgtct
     4501 gtttcaaaat ggtttgctgc aatgccgtct gaagcggccg tatcggttta gaagcgcgtt
     4561 cccaagctga tgtgccagcg gattttcttg tcgctgtgcc cgtaggcgat gtcgaaggaa
     4621 aacggcgcga gcgggctgaa ccagcgcacg cccagtcccg aaccgtgttt cagcttcata
     4681 cgtttgaaat tggcggcggc gtcgcccata tcgtggaaca ccgcgccgga aagggtgcgc
     4741 gtaaacggca gttggtattc caggctgccc accaagaggg cgcgttcggg caggaccgat
     4801 ccgttcgggc cggcaagccc gatgctgtcg agttcgtaac cgcgcacgga agacgcgccg
     4861 ccgctgcgga acatcagccc tgaaggaacg ttggcattgt cgcgggcaac ggtgtaaccc
     4921 gcttgtccgc gtatgatgaa cgtgccgagt tttttgtttt cgggcgtgaa gaaataacct
     4981 gcgcgggcag aggtgcggat cagcgcggtg gaggacagga atgcgcccaa agtcgtaccg
     5041 attttgccgt cgaggtaatg gccgttttcg ggatgcagca cgttgttgag cagctggcgt
     5101 ttccaagagg cggtcagcat cgtggcgtgg ctgttgccca aatcgatatc cgagccgggg
     5161 attttccggc cttctgcgag aaactccgcc cccagcctgg catcgatgcc cgcgcggtcg
     5221 cgcacatacc agatgccgcc ggagaaggcg cgtttttcga ggttttgggt ggtcgaacgg
     5281 ttgtaggaaa cgttgcttgt ccagtagttg ccccgatagt tgcgcggctg gctgatgccg
     5341 gcggcaagcg tggtttcgta tttgtccata tcccagacga ccgaaccgat atagcctttg
     5401 ttgaagaggt tgtaatagtc gtaggcgatt ttgccgccca aaccgtattc cgaatcgagg
     5461 cggatgccgg tttcgagctt gtggcgtttg acctcggtta cgctgacttt gacggggacg
     5521 cggtcgcctt ggaggcggtc gaagtcggct tgtacggacg cgccggaata atgcccgttt
     5581 tgttcgagcg cctgttggaa gtcgagcagc aggtcgaggt cgtagggcgt gcccggttgg
     5641 aagcgcgcca ggccggagac gatttgttcg gggtaacgct gcgtgccggt aatttcaaag
     5701 tcgccgaagg cgatggggcg gccgctgtcc acgacgacgt tcaaatcggc ggtggcggta
     5761 tcggggttga cggccgcccg ggtgttgccg agcttggcaa gcgggtaggc tttgcgcgtt
     5821 accgcgccga ggacggaagt tttgctgttt tcccaactgt cctgatcgaa atcactgcct
     5881 accggctgct gccagttttc cagcgcgttg cggtagtatt cggcgaggtt gccgtctgaa
     5941 aggatgtcgc cgaggatggc gacgccgacg ttggcgattt tggtgcgcgg gcccggtgtg
     6001 atgtgtaccg tataagctcc gtctttttcc gtcaggctga ctttgctgct gaaatagcct
     6061 ttgctgcgga gcatcgtttt aacgttgtcc gatgcttctt cggcgaggaa gcccgtctgt
     6121 tctttgtcca atacttcttc ctgctgctgc gtgatgagcg gcaggtgttc ttcgaccata
     6181 tctttgattt cactgtcctg cgtgtcgatg cggacgggga atttgggttt taatttgact
     6241 gattcggtgt cggggctttt gtttttgaac aatgcgaaac ccgccgcctt gttttcggaa
     6301 aggtcggcgg caggcgcgta tgcgtgcgga aagaaaaata aagccggcag gagcagggcg
     6361 gtcggtttaa tcatcatggt acgggtatcg tgcatcggtg tggcggattt cgattctaac
     6421 actgttcgga cggggcggga cgcttcagac ggcatactgc cgccggtatt tcggaggttg
     6481 cggcccgctg gaaatgtatc tgcttgtttt aaggtatttt gtttctaatc cgcatatgga
     6541 aaacaggctg cgtttcggaa tagtgtcgga aaataaacgg gattaaataa atttattttc
     6601 gttcaataaa ttaaaccgtc gcgccgaatg ataaagcaaa gcctgtccgg cactgcatag
     6661 ggcaaatcaa cgcaaccttc atagtggatt aacaaaaacc agtacggcgt tggctcgcct
     6721 tagctcaaag agaacgattc tttaaggtgc tgaagcacca agtgaatcgg ttccgtacta
     6781 tttgtactgt ctgcggcttc gtcgccttgt catgattttt gttaatccac tataaaacac
     6841 agccgaaaaa taaaaaaaac acagagaatg agttttcttt taaaatacaa cattttttaa
     6901 catatcacaa cataaggaag aattatggcg ttcggcaaat ccttatttca tgcgatcggc
     6961 cgcgtcagcc tggtcaggca gattgccgcc ggtttggcgt tgggcatcgt aatcggttcg
     7021 gtttccccgc aactgggctt ggcggcaggc ttgttcggca gcctgtttgt cggcgcgctc
     7081 aaagcggtcg cgccggtttt ggtatttatt ttggtggcgg ccacaatcgc gcagcaccaa
     7141 aaaggcaaca aggcgcatat caggccgatt atcatccttt acctcatcgg tacgttttcc
     7201 gcagccctga ccgccgtcat cgccggtatg gttttcccga cgcacattgt tttggcgggc
     7261 gcgggcgatg tgtccgccgc gccgccttcc ggcattgtgg aagtattgaa atcgctgctg
     7321 atgaacctgg tcgccaaccc gattaacgcg attgccaatg ccaactatat cggcattttg
     7381 gcttgggctt tggttttggg cgcggcgttg cggaatcacg gttcggacgt tacgcggcag
     7441 gtcgttgccg atttggcgga agcggtttcc accgtcgtga aatggattat ccgttttgcc
     7501 cctttgggca ttttcgggct ggtgtcgtcc acaatcgcgg agacgggttt cggcgcgctg
     7561 gcgggttatg cgaagctgct tgcggtactg ttgggctgta tggcgtttat cgcgctggtg
     7621 gtcaatcccg ccatcgtgtg gtggaaaatc cgccgcaacc cttatccgct ggtgtttacc
     7681 tgcctgcgcg aaagcggcgt gtatgccttc tttacccgtt cttccgccgc caatatcccc
     7741 gtgaatatgg ctttggcgaa aaaactgggg ctgcacgaag acacttattc gatttccatc
     7801 ccgttggggg cgaccgtcaa tatgggcggc gcggcgatta cgattaccgt tttggctatg
     7861 gcggcggcgc acactcaagg catacaggtt gattttgcca ccgcgctgct gttgagcctg
     7921 gtggcgacgg tcagcgcgtg cggcgcgtcc ggtgtggcag gcggttcgct gctgctgatt
     7981 ccgctggcgt gcagcctgtt cggcatcagc aacgatgtcg ctatgcaggt ggtcgccgtc
     8041 ggctttatca tcggcgtgat tcaggattcg gcggaaacgg cgttgaattc ttcaaccgat
     8101 gttttgttta ccgctgccgc cgatttgggt cgacagagaa accgggcgga ataaaccaag
     8161 ctatgccgtc tgaagcccgt ttggcgttca gacggcatat ttttacaatt ttatgggcat
     8221 atggcggaaa caataccggc tgccttgaat atgaagggga tgatgatgtt taaacgcagt
     8281 gtgattgcaa tggcttgtat tgttgccctt tcagcctgtg ggggcggcgg tggcggatcg
     8341 cccgatgtta agtcggcgga cacgccgtca aaaccggccg ctcctgttgt tgctgaaaaa
     8401 gagacagagg taaaagaaga tgcgccacag gcaggttctc aaggacaggg cgcgccatcc
     8461 acacaaggca gccaagatat ggcggcagtt tcggcagaaa atacaggcaa tggcggtgcg
     8521 gcaacaacgg acaaacccaa aaatgaagac gagggaccgc aaaatgatat gccgcaaaat
     8581 gccggcaata cggctgccca aggtacaaat caagccgaaa acaatcaagt cggcggctct
     8641 caaaatcctg ccccttcaag caatcctaat gccacgaatg gtggcaattt tggaagggtt
     8701 gatttggcta acggtgtttt gattgacgga ccgtcgcaaa atataacgtt gacccattgt
     8761 aaaagcgatt cttgtaatgg tgataatcta ttaagcgaag aagcaccgtc aaaatcagaa
     8821 tttgaacaat taagtgatga agacaaaatt aagaaatata aaaaagatgg agaaaagttt
     8881 accggtttgg ttgctgatag gttacagatg aaaggaacca atcaatatat tattttttac
     8941 aaacctaaaa ccacttcatc tgcgcgattc aggcgttctg cacggtctag gaggtcgctt
     9001 cccgccgaga tgccgctgat tcccgtcaat caggcggata cgctgattgt cgatggggaa
     9061 gcggtcagcc tgacggggca ttccggcaat attttcgcgc ccgaagggaa ttaccggtat
     9121 ctgacttacg gggcggaaaa attgtccggc ggatcgtatg ccctcagtgt gcaaggcaaa
     9181 ccggcaaaag gcgaaatgct tgctggcacg gcagtgtaca acggcgaagt gctgcatttc
     9241 catacggaaa acggccgttc gtacccgacc agaggcaggt ttgccgcaaa agtcgatttt
     9301 ggcagcaaat ctgtggacgg cattatcgac agcggcgatg atttgcatat gggtacgcaa
     9361 aaattcaaag ccgccatcga tggaaacggc tttaagggga attggacgga aaatggcggc
     9421 ggggatgttt ccggaaggtt ttacggtccg gccggcgagg aagtggcggg gaaatacagc
     9481 tatcgcccga cagatgcgga aaagggcgga ttcggcgtgt ttgccggcaa aaaagagcag
     9541 gattgatgtt gatgccgtct gaagcgaggg cgttcagacg gcattgtgat gtttgggtcg
     9601 cgggtccggt tcaagtccgc atcagttgat aaacccgtcg tctttcattt tcttttcgga
     9661 aaaatattga ccgcatacca gtaaggcggc gaggatgatg acaaacagcc ccgccatagt
     9721 cagtgtgatt ttgaagaaca attccgcttc gagaaaagaa aaccaaagtt gcacaatcag
     9781 cagcagcacc cagccggcaa acagtccgag acaggcataa acgccgagct taaacacggt
     9841 cccgctccaa aatgacgacg taggcctcgc gtttgaaaaa caacagcata cggcgcaaat
     9901 ctttttccat cgttacaacc cgccagcctt cgggggtttg tttgttgagg aacgcgctga
     9961 aattgacggg gttgaccttt gccgcgccga aaaacaggct ggacaacagg ctttcctgat
    10021 aaatgacgac tttgtattct ttcataggat tccttttaac ataagggatc atcttttcag
    10081 acgacccctt aagttgctaa tgatgcaaaa cagcaaacct tatttcttat tgcccaactg
    10141 cggcaatacc gaaccttcgc ccaatgccaa caaaccgctt tgcgagtaaa tgcccagttt
    10201 ggcgcgggtg tcggtaatgt cgaggttgcg catagtcagt tgtccgatac ggtcgagcgg
    10261 tgtgaacgct gcgtcttcga ctttttccat actcaggcgt tcgggttggt aggtcaggtt
    10321 gggcgattcg gtgttcagaa tcgagtagtc gttgccgcga cgcaattcga gggttacttc
    10381 gccggtaatg gctttggcaa cccagcgttg ggcggtttcg cgcaacatca gggcttggct
    10441 gtcgaaccag cggccttggt agagcaaacg tccgaggcgc aggccgttga tgcggtattg
    10501 ttcgatggtg tcttcgttgt ggataccggt caccaaacgc tcgtaggcga tgtggaacaa
    10561 cgccattccc ggggcttcgt agatgccgcg cgatttggct tcgatgatgc ggttttcgat
    10621 ttggtcgctc atacccagac cgtggcggcc gccgatgcgg ttggcttcga ggaagagttc
    10681 gacgggatca gcgtattctt tgccgttcag tgcaaccggc acgccttctt caaagcgcac
    10741 gctgacttct tcgggtttga tttcgacgtt ttcgtcccag aaggcaacgc ccataatagg
    10801 tttgacgatt ttgatgcccg agttcaaaaa ttccaaatct ttggcttcgt gggtcgcgcc
    10861 gagcatattg gaatcggtgg aataggcttt ttccaccgac attttgtagt tgaaaccgtt
    10921 ggcaatcaaa aattcgctca tttcatgacg gccgccgagt tcgtcgataa attgctggtc
    10981 gagccagggt ttgtagattt tcagcgcggg attggtgagc aaaccgtagc ggtagaaacg
    11041 ctcgatgtcg ttgcctttgt aggtgctgcc gtcgccccaa atattcacat cgtcttcttt
    11101 catggcggaa acaagcatag tgccggttac ggcgcgaccc agaggcgtgg tgttgaagta
    11161 ggcgatgccg ccggtggaaa cgtggaacgc gccgcattgg atggcggcga tgccttcgtg
    11221 tgccagctgt gtgcggcagt caatcaggcg ggcgttttcc gcaccgtatt ccattgcttt
    11281 tttgggaatg gcattgtagt cgtcttcgtc gggctggccg aggttggcag tgtaggcata
    11341 aggtagcgcg cctttgagtt tcatccacaa cagcgcggca gaggtatcaa gaccgccgga
    11401 gaaggcgatg ccgactttct gaccgacggg gaggttttgt aaaatagtat ggttttggct
    11461 cataagggta tccttcggac ttggaaaaag gagttcgtcg tgtttccttt tcgattgaaa
    11521 ccccgccact tggacatctg tccttcgggg cggtagaatc agactttatt tgggaggggc
    11581 gtaatccctt ccaaatcagg acggcacata gggcggtgct ttatgtgtcg tcccgtgtgt
    11641 tgaaacatta tcttataaat ataacccatt gtcagccgtt aaggctgtct gaaaagaagc
    11701 tttgcccgtt tcgctgaaaa tcttttcaga cggtctttac gccatcctca acctgactcc
    11761 ctcccctgcg ggggagggtt ggggagaggg tgcagacagc agcccttaag caaacgctgc
    11821 ctgaaaaacg caagctcgac cctctcccta accctctccc atgggagagg gaacaaatca
    11881 cagcaccatc gacattttca gacggcatcg gtaaagaaac ccatctatga acgcgttcaa
    11941 cctcaccatc atcggcgacg aaatcttaca cggcagccgc caagacaagc atttcgcctt
    12001 tttcaaatcc ctgctcgaat ccaaagggct gaagctcaat caggtgcaat atctgcccga
    12061 cgaacccgat ttgctggtca ggcaactgcg ccgcagcttt tcagacggca taccgacgtt
    12121 tgttaccggc ggcatcggcg ccacgcccga cgaccacacg cgccaagccg cagcggctgc
    12181 tttggatttg cctgtcgtcc gccatcctga agccgccaag tttatcgaag gcgtaacccg
    12241 gaaacgcggt gagccgctcg attcgcccga acacgcccaa cgcctgaaga tggcggattt
    12301 tccccaaggc gcggaattgg tgcctaatcc gtttaataat atcgccggat tttccattcg
    12361 cgagcattat ttcttccctg gttttcctgt gatggcgcac ccgatggcgg aatgggtttt
    12421 ggatacttat tacgtcggcc gcttcaacca aaccaaacgc ggcagccgca gcgtgtatgt
    12481 gtttgagcag cccgaatcgc gcattacgcc gcttatggag catatcgagc aaacttatcc
    12541 cggtgtgcgt tcgtacagcc tgcccagcgt cggttggacg cattcagacg gcacgcaggt
    12601 caaaccgcac attgagttcg gcatcaaagc cgaaggagaa gccgtcaacc ttttggacgc
    12661 ggcgtgggcg gaagtgttgc acagcttgga cggattggga gcagagctga aaaatcgggt
    12721 aaactgaaaa ccgtttgccc aaatcggacg atatttaagg aaaaccatgc aataccgaat
    12781 cagaagagaa aacgaagcac ccgaagcaaa aaatacgggg gaaaccttat gggagcgcga
    12841 catcatgcgc gaagtgctgc tgtccgccta tcaggacagg cgcagggagc ggatgtggaa
    12901 aaacatctgg cgtgccgtca gcaccctgat tctggttgcc ctgattgcag gcattttccg
    12961 aaaagacgaa gccgcattgc agttggcggg caacacgccg cataccgcag tcgtcaacct
    13021 gtacggcgaa atcggcaacg gtgtagaaga ccaggtcaaa aaactcaaag acggtatgga
    13081 ggccgcctac aaaaacccgc aggcaaaagc catcgtcatc cgcgccaaca gccccggcgg
    13141 ttcgcccgtc gtctccaaca ccgcttttga agaaatacgc cgtctgaagg cgcagcaccc
    13201 cggcattccc gtttatctcg tggcggaaga tatgtgcgcg tccggctgct actacatcgc
    13261 ggcggcggcg gacaaaatct atgccgaccc gtccagcatc gtcggcagca tcggcgttat
    13321 cggcagcagt ttcgacgcga ccggcctgat ggaaaaaatc ggcgtgaaac gcagggttaa
    13381 aattgcaggc agcaacaaag gcatgggcga tccgttttcg cccgaaacgc ccgaacagtc
    13441 gaaaatctgg gaagaaatgc tgaccggcat acacggcgaa ttcatcaaag ccgtcaaaac
    13501 cggacggggc ggccgcctca aattccgaca atatcccgac gtgttcagcg gtcgcgtgta
    13561 cacaggcgcg gatgcgctga aagtcgggct ggtggacgga ctcggcaata tttacagcgt
    13621 ggcgcgagac gtggtcaaag cccccgatgt ggtggattac actccgaagg acgatttcgg
    13681 cagaatcttg ggcagacgct tcggcgcaga actcaaagca agcgtgcgcg aagccctgca
    13741 ggccgtccgg tagcgttgtt tacagtattt ctaagggaat accaaaataa aaatatacac
    13801 aaaaacactc acctccccag tatgctgcca ctaagtcagc cgcgtcacta tttaccgtgc
    13861 acttaaagcc gtaaaaggca ggttgtcccc gggaggtttt gtgcatcctg ataccgatcg
    13921 gttgttgttg ctcaaaggac agaaggccgc tgataaacga gattacctgt ttgtcgctat
    13981 tgacgatttt tatactctgc cattttgcta gacaaaaccg cagacagtgc tgccaagttt
    14041 ctgaccgaac atctggccga cccctgcttg tacctgattg agtacgttta ctctgacaat
    14101 gataggtaat ataaagagcc gtccaacatg ctttcggtgc agcttgttat aataatggga
    14161 ttggttggag gcttgcccga tttgcttgtc cgcagaccaa cggtaaggcg gagcgggtta
    14221 tccgtacctt gatggagatg tggcatgagg aacagtcgtt tgacagtccg gaacatcggc
    14281 aaaaggagtt gtgctgcttt attaatttag gggctaccct agatcactag ggaaattcaa
    14341 attaagttag aattatccct atgagagaaa gtcgtctaag ccggtataaa caaaataaac
    14401 tcattttgtt atcgtggcta tgatgtattg gatgcgggcg aatttagcca tttccgtatc
    14461 aatcacagca cacattttgc cgaacgacaa aaccatatta atggaactga gaacttttgg
    14521 aatcaagcaa aacgtcattt acgcaagttt aacggcattc ccaaagcgca ttttgagctg
    14581 tatttaaagg agcgcgaatg gcgttttaac aacagtgaaa taaaagttct tgttccattt
    14641 taaaacaatt agtaaaatcg agtttatcct agttgtccag ggcagcctca tctctttata
    14701 gatagctgct taccaagtat ccttaattgc taattcaagc caatcttcaa aactattaaa
    14761 aaattcatgg ttttctatat tatctaaatt aatgacaata accctagaat ttcctgttac
    14821 atcattacca tcaaaataag cttcccattc tccattacta gcgaatggga taagatttaa
    14881 atcaggcagg atagaatttc taaatatata agctacttct gccccttctg ttccataatt
    14941 ctcaaaccac cagtgaaaat tatgtggctg aatataaggt attaaagatg tatcgttaga
    15001 gatacgaaca tacttttcag gataacaaaa cctttcagga agcatattac taggatataa
    15061 attaaattca ttttaaataa tcatttcgtt accttaggat cataaatact accattagga
    15121 aactgggtag accaatttgg tggtgctagt tcattttcaa tttgtttttt aattctaaat
    15181 tgaatagctt ctccccatgt ttgcggcatc taatgctgct tgctctgcat caatattact
    15241 aaaaattgga ttagtctcaa aaatgaaaaa ttactgtttt ttcatttttt atagcatatt
    15301 caagtatatg aataatattt ttcactaagg gctctgtaga aaaatctgaa ttttgtaatt
    15361 tttcttttac agccaaaata ctttccttat ctattagcca atccccctca cttggtccaa
    15421 ataaccaatt atcggtaatt tcagaaacaa gtctccataa tccacgattt tgatattcaa
    15481 taatttgact ttccgtaatt tctacaactg tttcagattg aatgttttta taagcccaat
    15541 caatcatttc agtatattca ctaattgaaa cattgtcttt taaacagaaa attaacaggt
    15601 tgtgttcatt catttgtcta agttcttaat atgagtttgt tgttgtaaat aatcttttgc
    15661 ttgcttacct tgtaaattaa aggattaacc gcaccttgga tgatgccgcg aatatcatcc
    15721 atacggttac cccaccaaat tttattggtg tatcgttaat catttatttc aaaaaagtta
    15781 tttattatca tattatcagg gagcaggtcg ttaaaaaaaa ccccctcaaa ttccattaaa
    15841 ttagtattta tccagcacat aaacgaacca aagcgttctt tccctgagtg taaatattta
    15901 aaattaaact caaaataagt ttttttgctt agaaaattta atggtttttc tatagtgaaa
    15961 aaaaagccct tactcgtttt agttcgagat actattttaa ataaattttt attttcaata
    16021 aaataagaat attcatttac taagttgtta tgataaaaaa gtaattttat taaaatgttt
    16081 tcataatttt ctaggttcat ttctagctca atttaaaatg atttcattgt tgccgtacca
    16141 atgcgaaatt taccatcaac aaaatttccc ttcactgata caggtacgcc atcaacttgg
    16201 gtagttttat tttcaggctg cctgaaagaa agataggcaa cttattactc atcttacgct
    16261 agccctcaat aattgaatac gttgcactag aaccaattat ttctatgaat gtttgctcaa
    16321 acataaaaat atagtattta aagaattctc tattcaaagg aaaataactt tctttcaata
    16381 tccattccgc ataacactca tcaagaagtt caaaaaagat tgaccaccat tttcatgaag
    16441 caaagctcta tctgcatctt ccgaaattaa atccataaaa gtatctataa aatacgattc
    16501 atcaacaacc ctaaaatgaa taacatcttc aataaaaatt tttatggttt tatccgtatt
    16561 tctataacca actgctataa cttctacttt atcagagttt attgctgcat tatcaaaata
    16621 gaaatagcca gttgggaaat ctaagctttt taatttcata atttatcgtg tatttccata
    16681 tctgattttt atttttcggt tattactaat tgttatttca agtgttggtt ctccaccact
    16741 actatcatta cgagcattaa ccgcacgcct atcaggtaaa ttgcccgtta tcgttccatt
    16801 aggacgtgtt tgtacatttc gaacattcag actattaaaa tcatttagtg cgtcattgta
    16861 atcccccact ttatcatact gaacagcggg accttttgtt ttactccttt gagtagtatt
    16921 gctcaaaatg tctttgattt gtaaatcact gcgcacctta ccggtttcta tgcgagctgc
    16981 cacactactc gggctatagg ggtcgctatc aatcgcagga ttaccaccgc tggttttttg
    17041 tccgctagcc tgtttcccaa cctgctgtac tgtatcgcca atttcatcta ccgtacctaa
    17101 cgccttagtc tgcatatggc gaacagcagg ttttttatag ccggtatttt taacccaatc
    17161 ccatttagtc gggttaagtt ctactttttt acctctccaa accgtacctg cggcctctgc
    17221 tacggaaagg gcagtttggg ctgttgcagt tatattcgga tgggcatcag cccattgtct
    17281 ggcagagttg atactgtctt ttaccgcaaa agcactgtct tgtaatgcgg ttgcggccgc
    17341 aagttgtgct tcgggactta aatttcctaa attattaata ccttgtagag tctgctgcgc
    17401 agagatgtag caactgtatt tttaccccgt cgggtaaaaa tacagttgct acggctgctg
    17461 ggaaagcagt tactattttc ccagtggctc aaaattaatt gtttcagcag atatttttat
    17521 aataatccct acatcaataa taatttcact ataaaattgg tatttatata gctctatttc
    17581 gggtataaac aagggaaaca agctatcttc cttatcctca taatctacga aattatttgt
    17641 atctaattta aaattcaaaa tattactgca tttgatatat ccgaaataat taccttcaat
    17701 tgtatctgta aaagataatg taagggtatt tccattatcg gaaaagatta tttcataaag
    17761 gcttacattg tgaaaaatat tttttttcat ttgacaaaat atccagaatg atgacgatta
    17821 tttggcaatg gattgccttc ataatcttgt cgttgcggat attttccctg tacaccatcg
    17881 gttcgcggca ctctttgggt tcttataacc tctccatttg gacgaatatc tattttcacg
    17941 ccatcaggac ctttccatgt ttcatattga gcctcatctg aaccagtacg tctaaaacct
    18001 tgttcttgca atgcttgacg taattcatct cgcgtagttg gtctgataga tttaacaact
    18061 ggcttgtttc ctcctgccat ctcaccgtcc aacgtctgca taggtcgggc agcaggtttt
    18121 ttatagccgg tatttttaac ccaatcccat ttggtcgggt taaggtttac ttttttaccg
    18181 ccccaaaccg tacctgcggc ctctgctatg gcaagagcag tttgggctgt tgcagttata
    18241 ttcggatggg catcagccca ttgtctggcg gaattgatgc tgtcttttac cgcaaaagca
    18301 ctgtcctgta ataggctcgc ggcagcaagt tgtgcttctg gacttaattt tcctaaatca
    18361 ttaatacctt gtagagtctg ctgagcggct gtatctgagc cggtcagtgt aggttggatt
    18421 aaaacccagc attttcaaca tcttgatgtt ttactataaa tctattcttg tgcttccttt
    18481 gttagataaa tttcaacaca aaagatacgt atgcattctc taattttatt tgagataaat
    18541 aatgcattgc tttgtcgata ctgttaaata aattacttcc ctcgtaatac caattatcat
    18601 atgtttgata aaaataatta tctttcttga tataaatatc tccccccaat acaagtattc
    18661 tctcattgat aaataaattt aatatatcta ttgcaatagg gacgggtaat gcggcttctc
    18721 taattccaat tcctatcaaa gattcccctt gtctcgaata aatttcaaaa atttcattta
    18781 acatgttaat ctctatctac tatttttcaa tcggtataaa ccctattcac attttatttt
    18841 tcctaaaacc aaatcttcga ctaactgggt tgcataaata atatcattta tgattttaac
    18901 aggtaaatta tttgataggt aaccatggga ttccgtcatc attgatttct ctaggtgctt
    18961 atttatctga ttggtaagcc ttttgcgtaa gcttctttcg cctccgcaag tgtcatttca
    19021 ttaggtccac catctatatt agtttctcct gtattttgcc actgacaaac gtcacagata
    19081 tcatagtcca taacttcagt tccgcaaaca ggacaatgta gccatatttc tccgtcaatt
    19141 tcccatgtct tttttgaatt ctccatcata atacttcttt cctaagtttg gtttaaacat
    19201 tgttttgatt ttgaatgttt ttggattggc caaaacatag atattgttat tgatatcata
    19261 gcgcactcgt ctatgttccg tctgataacc taatatcttg tccgatgtct gttttgataa
    19321 caaatcagac gccatttttt ggtagtcttc aatggttata tcgccaaatt cttttccatg
    19381 agctttgaaa tgtccattta aagatttctc agtaggaaac ttggactttg tccatctgat
    19441 gcggtcttga attgttcctg tcggagaaat agatgtatca ggtttaacat atgaggcagg
    19501 taagccgata ttgttatcag agtgtgtagg gtgttgctgc gtacttggtt ttggtggctt
    19561 attcccacct gccatctccc catctaaagt ctgcataggg cgggcagtag gtgttttata
    19621 gccggtattt ttaacccaat cccatttggt cgggttaagt tctacttttt taccgcccca
    19681 aaccgtacct gcggcctctg ctatggcaag ggcggtttgg gcagtagctg taatgttcgg
    19741 atgggcatca gcccattgtc tggcggaatt gataccgtct tttaccgcaa aagtactgtc
    19801 ctataatgcg cttgcggcag caagttgtgc tttcggactt aaatttccta aatcattaat
    19861 accttgtaaa gtttgctgcg cagagatgta ggtcggatat ttgtatccga caaaaatatt
    19921 tgacgcatct atcgtttccg aaatgccgct gttggaaaat gtcggattcg agaatccgac
    19981 ctacggctat ttcgtttaag tcataaatag ccattcatgt tccttctgaa tttgcttttg
    20041 ctatccttag aaccaatcat ataaacatta tcatcattaa ttaatgttct aagtttatga
    20101 agcggtcaaa tctgtcaaac taccagagat gaagcatatc ctcctggaag taattctgtt
    20161 cgtgctgttt ccatatcttc tttgccatca tcagttactt ctctgtctaa ataaaatctt
    20221 acgataagtt tttgtctaat ttcatcaaat tctgccgtaa ttaatcttat attgatatga
    20281 atacacggtt catacctgct attagccagt caggtgtagt tggatgggga ttcattttta
    20341 ataatatctt caagcaagtg aaatgtaaga aaagcgtaga tacttgaagg ccggctatct
    20401 gattggcaaa aagaaatttc ggagataaag aatttccgtt tatatttttc ataaattaaa
    20461 tcaacacctt gttttacata aaaactaact tcctcaggaa ggatatttat aacattctct
    20521 ccttttactt ttatagttaa tggtttgatt tttatgtttt catgaatatc tgataggatt
    20581 atttctaaaa aattattttt atttccagtc attctggcta ttttatgtat atttccattg
    20641 tgttcgatta tcattttatt ttttccaagg atttccttct atattgatag tttatctaaa
    20701 gatttcattt aaaatatcct gactaatttc ttgtttatat aattctacaa ttttctgttt
    20761 ctgtgtattt gtataaaatt tttcgttggt atctgcttga tattttaatt gtctcaatat
    20821 ttccatgatt tcatattctg atttttcatc atattcttca atcatgtaat caacaaattg
    20881 ttttagaata tcaaaggctt cgtaactttc caaaatttta atatgtttat cggcaaaatt
    20941 ggacatatac cattcgtcag tgattggatt agtatttaaa tcatctaaaa aaacatttaa
    21001 atcatctaaa aaaacattta catcatctaa aaacatgact aatccttaaa cttaaaatct
    21061 gttttctcac cggttcttga gttttcaatg taatgtattt caattttgtt tacgttttga
    21121 acacgcttaa cccaaccgtc ttttgctaac caaccatttt tagtatcgga cataggaggt
    21181 attctcgcag gagttttgcc ctgtgggttt gccttaactt cttccattgc taattgttcc
    21241 tttaaattat taggtattgt acgaccggtt gatttttcaa cagattgtgt ccctattttt
    21301 aatgatttgt ttcccccagc catttcccca tctaaagtct gcatagggcg ggcagcaggt
    21361 tttttataac cggtattttt aacccaatcc catttggtcg ggttaaggtt tactttttta
    21421 ccgccccaaa ccgtacctgc ggcctctgct atggcaagag cagtttgggc tgttgcagtt
    21481 atattcggat gggcatcagc ccattgtctg gcggaattaa tgccgtcttt taccgcaaaa
    21541 gcactgtcct gtaataggct cgcggcagca agttgtgctt ccggacttaa atttcctaat
    21601 tc
//
LOCUS       DAWFBO010000019        21047 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFBO010000019 DAWFBO010000000
VERSION     DAWFBO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21047
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.019"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            208..1329
                     /gene="dnaJ"
                     /locus_tag="WGO87_000496"
     CDS             208..1329
                     /gene="dnaJ"
                     /locus_tag="WGO87_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0391897.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGEPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1525..3543
                     /locus_tag="WGO87_000497"
     CDS             1525..3543
                     /locus_tag="WGO87_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0391898.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(3587..4144)
                     /gene="rfbC"
                     /locus_tag="WGO87_000498"
     CDS             complement(3587..4144)
                     /gene="rfbC"
                     /locus_tag="WGO87_000498"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0391899.1"
                     /translation="MNIIDTALPDVKILKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKAWAQAEKLRLTLSR"
     gene            complement(4189..5079)
                     /gene="rfbA"
                     /locus_tag="WGO87_000499"
     CDS             complement(4189..5079)
                     /gene="rfbA"
                     /locus_tag="WGO87_000499"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0391900.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENF
                     RAVSIEEKPQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSL
                     SVQILGRGFAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVET
                     RAKPLEKTAYGQYLLRLIGK"
     gene            complement(5276..6358)
                     /gene="rfbB"
                     /locus_tag="WGO87_000500"
     CDS             complement(5276..6358)
                     /gene="rfbB"
                     /locus_tag="WGO87_000500"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0391901.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEF
                     IQTNIVGTFNLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLGGTDDLFTETAPYA
                     PSSPYAASKASSDHLVRAWQRTYGLPAIVSNCSNNYGPRQFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLE
                     ELAPEKPAGVARYEDLITFVPDRPGHDVRYAVDAAKIGRELGWKPQETFESGIRKTMQ
                     WYLDNKTWWQNVLNGSYRLERLGTGK"
     gene            complement(6359..7402)
                     /gene="galE"
                     /locus_tag="WGO87_000501"
                     /pseudo
     CDS             complement(6359..7402)
                     /gene="galE"
                     /locus_tag="WGO87_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(7395..8397)
                     /gene="dcm"
                     /locus_tag="WGO87_000502"
                     /pseudo
     CDS             complement(7395..8397)
                     /gene="dcm"
                     /locus_tag="WGO87_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(8428..10701)
                     /locus_tag="WGO87_000503"
     CDS             complement(8428..10701)
                     /locus_tag="WGO87_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0391902.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPVRTMPGHRA
                     LAVLRGRNEGVLNIALKYRPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYKNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(10766..11416)
                     /locus_tag="WGO87_000504"
     CDS             complement(10766..11416)
                     /locus_tag="WGO87_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0391903.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            complement(11413..12210)
                     /locus_tag="WGO87_000505"
     CDS             complement(11413..12210)
                     /locus_tag="WGO87_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0391904.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(12210..13373)
                     /gene="ctrB"
                     /locus_tag="WGO87_000506"
     CDS             complement(12210..13373)
                     /gene="ctrB"
                     /locus_tag="WGO87_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ0391905.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(13388..14563)
                     /gene="ctrA"
                     /locus_tag="WGO87_000507"
     CDS             complement(13388..14563)
                     /gene="ctrA"
                     /locus_tag="WGO87_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0391906.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            14698..15831
                     /gene="neuC"
                     /locus_tag="WGO87_000508"
     CDS             14698..15831
                     /gene="neuC"
                     /locus_tag="WGO87_000508"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ0391907.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKCQSEKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            15835..16521
                     /gene="neuA"
                     /locus_tag="WGO87_000509"
     CDS             15835..16521
                     /gene="neuA"
                     /locus_tag="WGO87_000509"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ0391908.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            16522..17571
                     /gene="siaC"
                     /locus_tag="WGO87_000510"
     CDS             16522..17571
                     /gene="siaC"
                     /locus_tag="WGO87_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ0391909.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIS"
     gene            17723..20836
                     /locus_tag="WGO87_000511"
     CDS             17723..20836
                     /locus_tag="WGO87_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0391910.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLYPPFHLHPNVKTSSIIDLFNDIPENVSCRNTPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSAEDSIIFTHPLQLEMYRLANNDIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDDEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSESEGFPYIFMEGMVYDIPIVVYDFKYGAND
                     YSNYNENGCVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKSSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
BASE COUNT         5671 a         4797 c         4816 g         5763 t
ORIGIN      
        1 ataacaaaaa cacttcatgg cgggttggtt ttatgcctgt ctaagttttt gtgtcgtgca
       61 tacctgaaaa tttcagacgg catcggttta tgctgtctga aaagtgtatt ccgtttcagt
      121 ttgtaagcta tggcagtctg tttgtcttgt gttttgcgca attgccctta ttttgagccg
      181 tgattttatt ttgaattaga tgaaaaaatg agtaatcaag atttttatgc gacgctgggt
      241 gtggcaagaa cagctaccga tgatgagatt aaaaaagcct accggaaatt ggcgatgaaa
      301 taccatcccg accgcaatcc tgacaataaa gaggcggaag agaagtttaa agaagtacaa
      361 aaggcgtatg aaactttgtc cgacaaggaa aagcgcgcta tgtacgacca gtatggtcat
      421 gcggcgtttg aaggcggcgg acaggggggc ttcggagggt ttggcggatt tggcggtgcg
      481 cagggttttg actttgggga tattttcagc caaatgtttg gaggcggttc ggggcgcgcc
      541 cagcctgatt atcagggtga ggacgttcaa gtcggtatcg aaatcacgct tgaagaagcc
      601 gcaaaaggtg tgaagaaacg catcaatatt ccgacttatg aagcgtgtga tgtctgtaac
      661 ggcagtggcg cgaaaccggg gacatccccg gaaacctgcc cgacttgcaa aggttcgggt
      721 acggtgcaca tccagcaggc gattttccgt atgcagcaga cttgtccgac ctgccacggt
      781 gcgggcaaac acattaaaga accttgcgtc aaatgccgtg gcgcggggcg gaataaggcg
      841 gtcaagacgg tggaagtcaa tattcccgcc ggtatcgatg acgggcagcg tatccgtttg
      901 agcggcgaag gcgagccggg tatgcacggt gcgcctgccg gcgacctgta tgtaaccgtc
      961 cgcattcggg cgcataagat tttccaacgc gacggtctgg acttgcattg cgaactgccg
     1021 atcagttttg ccacggctgc tttgggcggg gagttggaag tgccgacctt ggacggaaag
     1081 gtcaagctca ccgtccccaa agaaacccaa accggcagga ggatgcgcgt gaagggtaag
     1141 ggtgtcaaat ctttacgcag cagcgcgacc ggcgatttgt actgccatat tgttgtcgaa
     1201 acgcctgtca atttgaccga ccgtcaaaaa gagcttttgg aagaatttga gcggatttct
     1261 accggcttgg aaaaccaaac accgcgcaag aaatcgtttt tagacaagct gcgcgatttg
     1321 tttgattgat tttaaggttc ggaaacaagc agccgtatcg gggaatctcc ctgatacggc
     1381 tgtttttgtt tgtttaaaaa tagtttttat tttcaatggg gtatggggca gggtgggata
     1441 actgttttta actgttcttt ttaaaacttg acatcatggc gcgatgccaa caatatgtga
     1501 acgtctgttg tcaaaggaag aataatgaat aaatctttat ccgggtcggt agaagaatac
     1561 cgcgaactga cgctccgagg catgatactc ggtgcattga tcactgtaat ttttactgcg
     1621 tccaacgttt acctcggttt gaaagtcggg ctgacctttg cctcgtcgat tccggcggcg
     1681 gtgatttcga tggcggtttt aaagtttttc aaaggcagca atattttgga aaacaacatg
     1741 gtgcagaccc aagcttcggc ggcgggtacg ctttcgacca tcatcttcgt cctgcccggt
     1801 ttgctgatgg cgggctactg gagcggtttc ccgttctggc agacgacgct tttatgtatt
     1861 gccggcggga ttttgggggt gattttcacc attccgctgc gttacgcgat ggtggtgaaa
     1921 agcgatttgc cttatccgga aggtgtggcg gctgctgaaa ttttgaaagt gggcggtcat
     1981 gaagaagggg ataaccgtca gggcggcagc ggcatcaaag agctggcggc cggcggtgcg
     2041 ttggcgggat tgatgagctt ttgcgccgga ggtctgcgcg tgattgccga cagcgcgagt
     2101 tattggttta aaagcggtac ggcgattttc cagctgccga tgggcttttc actggcattg
     2161 ttgggcgcgg gctatttggt cggactgacg ggcggtatcg ccatcctgtt gggcatttcg
     2221 attgcttggg gcattgccgt gccgtatttc tcctcacaca ttccgcaacc ttccgatatg
     2281 gaaatggcgg cgtttgcgat gaagctgtgg aaggagaaag tgcgttttat cggtgcgggg
     2341 actattggca ttgcggcggt ttggacgctg ttgatgctgc tcaagccgat ggtggaaggc
     2401 atgaagatgt cgttcaagag ttttggcggc ggtgcgcccg ctgcggaacg cgccgaacag
     2461 gatttgtcgc ctaaggctat gattttttgg gtgctggcga tgatgtttgt tttaggcgtg
     2521 tcgttttacc actttatcgg cgattcgcac attacgggcg gcatggcttg gcttttggtg
     2581 gtcgtttgca cgcttttggc ttccgtcatc ggctttttgg tcgccgccgc ctgcggttat
     2641 atggcaggtt tggtcggctc gtcttccagc ccgatttccg gcgtgggcat cgtgtccgtc
     2701 gtcgttattt cactggtttt gctgctggta ggcgaatccg gaggtttgtt ggcggatgag
     2761 gctaaccgca aatttttgct ggcactgact ttgttttgcg gctcggcagt aatctgcgtg
     2821 gcttcgattt ccaatgacaa cctgcaagac ttgaaaaccg gctacctgct caaagccacg
     2881 ccttggcggc agcaagtcgc cctgattatc ggctgtatcg ttggtgcgct ggttatttcg
     2941 cccgtgttgg aactgcttta cgaagcctac ggctttaccg gcgcaatgcc gcgcgaaggc
     3001 atggatgcgg cgcaggcttt ggcagcccct caagcgactt tgatgacgac catcgcgtcg
     3061 ggcattttcg cccacaacct cgaatgggtc tacatcttta ccggcatcgc gattggcgcg
     3121 gtgttgattg ccgccgattt ggtgttgaaa aaatcatcag gcggcaaact tgccctgccc
     3181 gtccttgcgg tcggtatggg tatttatctg ccgccgtccg tcaatatgcc catcgtggca
     3241 ggcgcggtgt tggcggcggt gttgaaacac atcatcggta aaaaagcgga aaaccgtgaa
     3301 ggccgtctga aaaacgccga gcgcatcgga accttgttct ccgccggcct gattgtcggt
     3361 gaaagcctga tcggtgtgat tatggcgttt attattgcct tctccgtgac caacggcggc
     3421 tcggatgcgc cgcttgcgtt gaatctgcaa aactgggatg ccgccgcctc atggttgggc
     3481 ttggcgttct tcgttaccgg gatgtttttc tttgcacagc gcgtactgaa ggcgggtagg
     3541 tagcctgtcg gaaaaaatgc cgtctgaaac gttcagacgg cattttttat cgggaaagcg
     3601 taaggcggag cttttcggct tgcgcccacg ctttaccggc aagatctttg ggcgacagca
     3661 gcggcgcggt ttgaagcggc cagcctatgc cgactgtcgg gtcgttccat attaaaacct
     3721 gttcggcttc aggcttgtaa tagtccgtgc atttgtacac gacttccgct gcgtcgccca
     3781 aaacgcaaaa cccgtgcgcg aagccttcgg gtatccaaag ctggcattgg ttttgcgcgg
     3841 acagggttgc gcccgcccat ttgccgaaag tcggcgaacc ttcgcgcata tcgacggcca
     3901 cgtcgaacac ttcgccgaca actacgcgta cgagtttgcc ttgtgtgttt tcggtttggt
     3961 agtgcaggcc gcgcaatacg cctttgccgg atttggagtg gttttcctgc acaaaggttc
     4021 ggtcggcaat attttctttg aaccacccgt cgcggaaggt ttccataaaa aagccgcgcc
     4081 cgtctgtgaa gacttgcggt tttaaaattt ttacgtcggg gagggcggtg tcgatgatgt
     4141 tcatgatttt ctctgtatgc cgtctgaaga aagtggttca gacggcattt atttgccgat
     4201 taggcgcagc aggtattgcc cgtaggcggt tttttccaaa ggctttgccc gtgtttccac
     4261 atcttttttc gtcagccaac cgttgcgcca ggcgatttct tcgaggcagg cgatgtgcag
     4321 gttttggata ttttgcacgg tttggacgaa tgaagcggct tcgtgcaggc tctcgtgggt
     4381 gccggtgtcc aaccatgcga aaccgcgtcc taatatttgg acggacaatg aaccgtcttc
     4441 caaatacatt tggttgaggt cggaaatttc caattcgccg cgtgcggacg gtttgagctg
     4501 tttggcgaac tcgacggcgc ggttgtcgta gaaatacaag ccggttaccg cccaatcgga
     4561 tttgggccgt tgcggttttt cttcgatgga aacggcgcgg aagttttcgt taaattcaac
     4621 cacgccgaaa cgttcggggt ttttgacctg ataagcaaac acggttgcgc cgtgcgtttg
     4681 cgctgccgcc tgtttcaatg tttgcgctgc cgcctgtttc aatgtttgcg taaacgactg
     4741 accgtaaaaa atattgtcgc ccaaaaccaa gcaaacattg tcgttgccga taaattcttc
     4801 gccgatgata aatgcctgtg ccaagccgtc cggactgggt tgcacggcat aactgatgga
     4861 aatgccgaaa tcgctgccgt cgccaagcag gcgtttgaaa gaggcgttgt cttcaggcgc
     4921 ggtaatcacc aaaatatcgc ggattcctgc cagcatcaaa accgacaagg ggtaataaat
     4981 catcggtttg tcgtacacgg gcaggagctg tttggatacg ccgcgcgtga tggggtagag
     5041 gcgcgtgccg ctgccgccgg caaggatgat gcctttcatc gtatttcctt ttcggttgaa
     5101 accccgccct ttagggcggt agaatcagac tttatttggg agggggtaac cccttccaaa
     5161 tcaggacggc acatagggcg acgctttatg tgccgtcctg tgtgttgaaa catcttttct
     5221 ttcttccttt gcgataggtt ttcagacggc attgcgtcgg gatgccgtct gaaaactatt
     5281 tgcccgtacc taaacgttct agacgatagc taccgttcaa tacattttgc caccaggttt
     5341 tgttgtccaa ataccattgc attgttttgc ggatgccgga ttcgaatgtt tcttgaggtt
     5401 tccagcccag ctccctaccg attttggctg cgtcgacggc gtagcgcaca tcgtggccgg
     5461 ggcggtctgg gacaaaggta atcaaatctt cataccgtgc cacaccggcc ggtttttcgg
     5521 gagcgagttc ttccagcagg gcgcagatgg ttttgacgac ttcaatattg gctttttcat
     5581 tgtggccgcc gatattgtag gtttcgccga caacaccttc ggtaacaacc tgatacagtg
     5641 cgcgcgcgtg gtcttcgaca aacagccagt cgcggatttg cataccgtcg ccgtaaacgg
     5701 gcagcggttt gccgtcgagc gcgttcagaa tcatcagcgg gatgagtttt tcggggaatt
     5761 gtcggggccc gtaattattg gaacagttgc tgacaatggc gggcagcccg taagtccgtt
     5821 gccacgcgcg gacaagatga tcggaagatg ctttagacgc ggcataagga ctggacggcg
     5881 cgtatggcgc ggtttcggta aacaaatcgt ccgtgccgcc taaatcgcca tagacttcat
     5941 cggtggaaat atggtggaaa cggaaggctt cgcgcttttc agacggcatt tgttgccagt
     6001 aggcgcgggc tgcttcaagc agattgaatg tgccgacgat attggtttgg ataaactcgc
     6061 ctgccgaacc gatagagcgg tcgacatggc tttccgccgc caagtgcatc acggcatcgg
     6121 gccggtgttg tgcgaatacg cggtcgagtt cggcgcggtc gcaaatgtcc acttgctcga
     6181 aagcatagcg gggattatcg gcaatgtcgg tcagcgattc gagattgccc gcgtaggtca
     6241 gtttgtcgag gttgacgacg gaatcttggg tgttttgaat gatatggcgg acaacggcag
     6301 agccgataaa gcccgcgccg ccggtaacga ggatggtttt tttgtttgcg gtttgcattt
     6361 aatccccata tccgttgggg ttgcggctga cccaacgcca cgaatcctcc atcatttgcg
     6421 caaggctgcg tttggtttcc caacctgtct cttgtttggt atgggacggg tcggcaaagg
     6481 aacacgccaa gtctccggcg cgtcggggtt ggatttgata gggaatattt aagccggatg
     6541 cggcttcaaa ggcacggatg atttccaaaa ccgaataggc gcgccccgaa cccaagttga
     6601 acaaatgtac gccggcaaca ccgcctttcg ccttcattgc cgcgatatgc ccttctgcca
     6661 aatccatcac atgaatatag tcgcgcattc ccgtaccgtc cggggtcgga taatcgccgc
     6721 cgaataccga cagttgcggc agcctgcccg aagccacttg gcagatatag ggcaaaaggt
     6781 tgttgggaat accgttgggc tgttcaccga tcaagccgct ttcgtgcgcc ccaatcgggt
     6841 tgaaatagcg caacaaaatc acgctccaac gcggatcggc cttttggatg tcggttaaca
     6901 tccgttccac catcgctttg gatgcgccat aaggattagc ggtatcgccc gggcgcatat
     6961 cttccgtata gggcatttta cgtatcatgg cgcgacgcat taaagtaaac gccaaagcgc
     7021 gtttatactg gttatccagt ttccctatat ttttacgtta caaatcaagt tgttggtatt
     7081 catcgtgaaa atagaatttt tcggcataac gctgactcat aaaaccggca tgcgggctgc
     7141 ctgaaaaaat cattgcgaca tcatcgtcat ttaatgtttc atggttgttt tctattaatg
     7201 ctaaagcaat ttggtaatta attgccaaaa tatcgttagt aatcacacga tagccgcgtt
     7261 ttttggcttc gtaggcaaag gaacagcctc cactaaatac atctgcaact gtatctacat
     7321 cagacggaag ctggtcacaa atccaggaag ctattttctc tttattaccg atatagttaa
     7381 ttttcggata ttgcttattc attttcactc atttttaaaa tactttcagc aatagctttt
     7441 gccaataaag gcggtacggc gttaccaacc tgctgctgct gggcaatttt gctgccgcaa
     7501 aaaataaaat tatcagggaa agattgtaag gcagctaatt cacgaacggt taacgcccga
     7561 ttctgttcat agtgaaaaac tttgcgcata tctcctgtaa tacaaacggc tggttttgtt
     7621 gctgttgtaa cggatgtatt tacggatatc acctgttttc ggacgtaatg gttcaggaat
     7681 atcgttacgg ttacctccat ttttaacaaa tgccattttt tctaacattt gtgccgaatg
     7741 attcatagct tcatgatttg caacgtgtgg attgctttcg ccagcagcca gttttggaaa
     7801 atgtcctatt gctgatccaa cagtctgatg ggaaatctgc aaaggttcgg gaaaggaaat
     7861 tttgccttta tccctcctcc cgataaatat cactcggcta cgtatctgag gaacaccgaa
     7921 atcggctgca ctcagtatct tacattccac cgaataaccg atattctgaa atgcttgaat
     7981 aatctcaata cgtgttttac ctgaattgtg tgtatagagt cgcgctacat tttccataac
     8041 aaaaaaatat ggttggacaa ttttaactat tcggacaaac tctttaaata aatggttgcg
     8101 tgggtcatct gtaaatgtcc gtccaatctt tcctgccata ctaaaacctt gacaaggtgg
     8161 tcctccaata atcaaatcaa ctgcttgtcc gttaagacaa ttgattaaat cttgttcggt
     8221 tagtgtggtt aaatcttttt gcagtaattg atgatggggg aagttggtac ggtaagtctg
     8281 acaataatca gactccattt caacagaaag caattgttgg aatccggctt gttcaaaacc
     8341 caaggatagg cctcctgctc ctgagaaaag gtcaatatag gtaagtggtg tgtgcataag
     8401 tcaaaatcca taaactcttc gattatttta ccgcttcagc ttcgcaaacg catccgccat
     8461 cgccgaattg gctggggcgc ggtcgttgcg ttggggtttg cggccggctg tccgttcgcg
     8521 gctgcggttt tcagacggca ttttatgttt tgcgccgccc ggttcgtcat ccaagcgcat
     8581 ggtcagcgcg atgcgtttgc gtgcagcatc gacttccagc actttcactt tcaccacgtc
     8641 gccagctttc accacttcgc gcgggtcttg gacgaacttg ttggacaggg cggagatgtg
     8701 caccaagccg tcctgatgga cgccgatgtc cacgaacgcg ccgaagttgg cgacgttgga
     8761 aaccacgcct tcgagtatca taccgacttg caagtcgctg atttcgtgga taccttcggc
     8821 aaacgatgcc gtctgaaact cgccgcgcgg atcacggccg ggtttttcca gttcggacag
     8881 gatgtccaaa atggtcggca ggccgaagcg ttcgtcggtg aagtcggacg ctttgatttg
     8941 cttcacgcgc tcgcggttgc cgatgagttc ggcggcgcta atgccttgtt gcgccagcat
     9001 tttggcgacg acgggatagg cttcggggtg gacggcgctc gcgtccaacg gctctttgcc
     9061 gccgttaatc cgcaaaaagc ccgccgcctg ctcgaaggtt ttttcgccca aacgcggtac
     9121 tttcagcaat tttttgcggc tgtcgaacgc gccgttttca tcgcggtagg cgacaatgtt
     9181 ttgggcaagg gtttgattca aaccggaaat tcgcgccaag agcggggcgg aggcggtgtt
     9241 cacgtccacg ccgacggcgt tcacgcagtc ttcgaccacc gcgtccagcg atttggcgag
     9301 ctggctttgg ttcacgtcgt gctgatactg ccccacgccg atggatttgg ggtcgatttt
     9361 gaccaactcg gcaagcgggt cttgtagcct gcgggcgatg gacaccgcgc cgcgcaggga
     9421 aacgtccaag tcggggaact cgcgcgccgc cagttcggac gcggaataaa tcgacgcgcc
     9481 ggcttcggac acgacgattt tatgcagccc cattttcgac atttcacgca ccagttcgcc
     9541 cgcgattttg tcggtttcac ggctggcggt gccgttaccg atggcgatga gcttcacgcc
     9601 gtgctgttta atcaggcgcg acagcgttgc caacatattg ttttcttgat gcaaatagac
     9661 gatgacggta tccagcagct tgcccgtgtc gtccaccacg gcacatttca cgccgttctt
     9721 gtatccaggg tctagaccca aagtggtcag ccgtccggcg ggtgcggcga gcagcaagtc
     9781 tttgagattt cgggcaaaca cggtaatcgc gtcggtgtcg gcggcttctt tcaaacgatt
     9841 gagagcttcg agttccaacg acaaaaagat ttttgcgcgc caagtcagac gcacggtgtc
     9901 gcgcagccat ttgtggccgt ctgaaacctt gaaacggcgg gcgatgattt gctcgtattc
     9961 gctttgctgc gtaatcggcg tgtcgtcggg ccggtatttg agcgcgatgt tcaacacgcc
    10021 ttcgttgcgg ccgcgcaaaa ccgccaacgc gcggtggccg ggcatagtgc ggacgggttc
    10081 gcggtggtcg aaataatcgc tgaatttttc gccttcggtt tctttgcctc caacaacttg
    10141 cgcgtggatt tcggcttcgt tccacagctt gtcgcgcagc gtgccgatga gttccgcgtc
    10201 ttcggcaaac tgctccatca gaatcgcacg cgcgccgtcc aacgcggctt tggcatcggg
    10261 gacgttttcg ttcaggtagc cttgtgcggc ggcttccacg tcctgcgact gctcggcaag
    10321 caacacgtcc gccagcggct gcaaaccgtg ttcgcgcgcg atttgcgctt tggtgcggcg
    10381 tttgggtttg tagggcagat acaggtcttc cagcgcggtt ttgttatcgg cggcttcgat
    10441 ttgcgccctg aggtcgtctg aaagcttgcc ttgctcttca atgcttttta aaacaacggc
    10501 tttgcgctct tccaactcgc gcaggtattg cagccgctcg gcaagccggc gcagctgcgt
    10561 atcgtccaac ccgcccgtgg cttccttgcg gtagcgggcg ataaagggca cggttgcgcc
    10621 gtcgtccaaa agctcgatag cggcattgat ttgcgcggta gtcgcggaaa gttcttggga
    10681 aagaatttga gtaatgttca tcaataaaat tccaacggac aggccgtctg aaatttcaga
    10741 cggcctgatt taaaaaacaa tcgctttaag gtagcgaatt ataatattcg taggctttgt
    10801 ccatatcttc aaactggtgc atatgccctt tttccagcac catcgcatta tcgcaatatt
    10861 gcttcatggc gctgtggctg tgcgacacca agatgatgga acggtctttg cgcttttcaa
    10921 acaactcgta cttacattta tcggcaaaac gcgagtcacc aactgcaatc acttcgtcaa
    10981 tcaaatagca atcaaactct accgccaacg acaaagcaaa tgccaaacgc gccttcatac
    11041 ctgaagaata acgcttgacc ggctcgtaaa gatattgccc caactcggaa aactcttcgg
    11101 taaagttctt cacataatcg atatcgacat tgtaaatccg gcagatgaaa cgcaaattgt
    11161 ccataccggt cagactgcct tgaaacgcac cggagaatgc caaaggccaa gaaatactca
    11221 ttgtccgctt gatttcaccc gtggtcggcg gctcaacgcc actgatcaaa cggatgagcg
    11281 tcgatttacc tgcaccgttg cggccgagaa taccgatttt ctcgcccttc tccattttga
    11341 agctaatatc gtgcaagact gtccgccaac cttggcgggt cagatagcgt ttggaaacgt
    11401 gttcaactga aatcattgcg gctcaacccc tttgctgaat ctcgccacca ccgccaaacc
    11461 aagcagcagc aaaaccaagt tacacaacaa aatataccaa ggattttcgt aagtcgtgac
    11521 gctgtcgcca aaatatccgg cacggaacat ttctgtgcca tgcaccatcg ggatcatcaa
    11581 tgcatactct tgcactttcg gaggcaaatt atgtacaaaa aagaatgcac ccgataacgg
    11641 catcatcaca aaagtcaatg tgccccaaat cttgccaaac ggctcgaaat taaaggcaat
    11701 cgaacaaatc accaaaccca aaccaatcgc aaaaaaagcc atcaaaagcc aagccatcag
    11761 catataaaac atatctgccg gcatttcaat ccagccaatc gcaatcaata ccgccataat
    11821 cacaatctgc gcaatggttg caccagcaat ttccaaaatc atgcgcgcca agatggtatc
    11881 caaaactctt acattgcggt gataaagcaa gctggcattt gaagaaatcg acccaactgc
    11941 ccgttttgag gcattacgcc acatcatcaa catcggatag ccagtaatcg caaatgcgac
    12001 aatattcaaa gttgaatatc ggtctgcctt taaaaatttc cacatcaaga cgataacgaa
    12061 tgtcatcagc aacggctcaa caaacagcca taaaaagcca atattattgc gaccgtaacg
    12121 ggtgataatt tcccgcatca aaagcgcacc gattacgcgc ctttgaatgg ctaaagattc
    12181 ccaaaatgat gttttatgca aggctttcat cagtttttat gctcacgaat gctggcagtc
    12241 aacaggctca aaataccata aaccatcaag ccgataatca gagtggcaac aatgttgtat
    12301 aaccgtttag gctcatgtgc caaatccggc aggctcggtt gcgagatcac ttccaaataa
    12361 agctgctgac ggtctgcttc aaccttggca ctttccaaag aagtcatggc ggctgccaac
    12421 tgctgctctg ccaactggtt ttccaaatac acacgctgat attcggcagc ctgattagac
    12481 aacgaagaat gcccaccgcc cgaaatggca cgtaactgtt ggtcaatttc tttacgcaag
    12541 ctctgctcac gcgcctgcaa acccggaatc tgcggattct ccggagtgac tgctttcacc
    12601 tgatccagct gggtttgaat cacaatcaat tcatcttgca gcttggaaac caaccccatt
    12661 tgcacttccg attgcgcttt caaatcaaaa acgccattgg caatccggta atccgtcaga
    12721 ttctgagagg cttcctttac ccgctctgcc gccgttttca ctacttcttc cgcatagcgc
    12781 accgtatcag cacgtgcacg atcgttcaac tggttaatca atgcttcacc ttgttttaac
    12841 aaagcctcat tgattttctt agattccagc gcatcaaagg aagttacatt caacgtggaa
    12901 atacccgaaa ccgtatcaaa attgatcatc acctgatttt tatagtattg ataaaaagcc
    12961 tcttcctcgc cacggaaccc aaacccatta aagcggctga acgcatcacc tttggtttca
    13021 taaaactcac gcaccggcaa gattttacgc agttcatcca aagacgagcg cgaacgcata
    13081 tactccccaa ccgtgtaaat atcatcttgc gcacgggcaa aacctgtgcc ctgcaaaatg
    13141 gcacccaggc cattgagaga agattggctt ttaggcgagc gcaccacaaa gctcgattgc
    13201 gacgtaaaac gatcggaagc gaagaagccg aaatacacca acgaaattac cgtagggata
    13261 atcaccgtta cccaaaataa agggcttagc tttttaatcc aacttttctt tttcggcttt
    13321 ttacgctcgg ctttggtttc agtggcaact gccacaggaa gttgctcaga catcttaatt
    13381 actcacatta attagttaaa ttattaatac tgttcgcgcc actggtaacc ggcgagaaca
    13441 caaacgacaa gaatttctgc acttcagcca acggcgcatt cgacacatac aatacatctt
    13501 tattcttcac aggaaagcgc tgcatagaaa atagcgaatg cgcatcagcc atattcacac
    13561 gatataccgt tggaatctct gcctcactgc cataaccttg agcaatccat ttatcctgac
    13621 gttctgccgg caattccacc aatggcgtat agcggaacac aaacacacca cgcgcatcag
    13681 aacggcgatc ttgcaaaccg cccatacggc caatggcttc agaaagcgat aagcctctgg
    13741 ctgaaaaacc gatttcttgt gttctcccca ccgcacccat agacgtaaag gtatagggat
    13801 tggtaatcat ggtaaccaca tcaccgcgac gcagcaaaat attttgtcgc ggatttgcaa
    13861 ctaaatcttc caaggcaaca gttcgtacta cattgccacg tgtcagctgc acattcgtat
    13921 cctgcacatt tgccgttgaa ccacctaccg cagccaccgc atccaacaca cgctcaccgg
    13981 ctgccgtcag cggcatacgc acactattcc cagcacgaat caccgacaca ttcgccgcat
    14041 tattctgcac caaacgcacc atcacttgtg gctgattggc catttttttc aggcggcctt
    14101 taataatttc ctgaacctga ccaggcgttt taccgaccac cgaaatatcg ccaacaaacg
    14161 gcacagaaac cgtaccacgt gccgtgacca actgctctgg caacttagtt tgatgcgcac
    14221 tacccgagcc catcgaagaa aggccaccac caaacaatac tgccggcggc gcttcccaaa
    14281 tcataatatc caatacatca ccaatattta gcgtaccagc cgaagcataa ccatcgccaa
    14341 actgagtgaa tgactgattt atctgagcct tatataataa ctgagcaacc gtatgattca
    14401 catcaatcag ctccacttca ggaatttgaa cttcagattg ttgccctaaa gagacaattt
    14461 tttttgcgct ggggcctgat gaaggaatcg cagagcatcc tacaattaaa cttccacaca
    14521 ataataatac tgcgtgacga atataaaatt tcactttaaa cacaagccaa atcctaatat
    14581 aattataaat ggcctaatta tagcacttaa tcgaaataaa tttatgagta cgtagagtat
    14641 aattagtatt cttctttcca acttccttat acttatatac ttatagattc taaaatcatg
    14701 aaaagaattc tttgcattac aggtaccaga gccgacttcg gcaagctaaa acctttatta
    14761 gcctatattg aaaatcaccc agaccttgaa ttgcatttga ttgtaactgg tatgcatatg
    14821 atgaaaacat atggcagaac ctgcaaggaa gtaactcgag aaaactatca acatacatat
    14881 ctgttttcaa atcaaatcca aggtgaacca atgggtgccg ttttaggcaa taccattacg
    14941 tttatctctc gtctatctga tgaaattgaa cctgatatgg tcatgattca cggcgaccgt
    15001 ttagaagcac tagcaggcgc aactgtaggt gcattaagca gccgtttagt ttgccatatc
    15061 gaaggtggtg aactatctgg tacagtagat gactccattc gtcattctat tagtaaactt
    15121 tctcatatcc acttggtagc aaatgaacaa gctgtcactc gcctagtgca aatgggagaa
    15181 aaaagaaagc atattcacat catcggctcc cccgatttag atgttatggc ctcttccacc
    15241 ctcccatcct tagaagaagt caaagaatat tacggtttac catacgaaaa ttatggtatt
    15301 tctatgtttc accccgtgac tacagaagca catttaatgc cacaatatgc ggcccaatat
    15361 ttcaaagcat tagaattaag tggccaaaat atcattagca tttaccctaa caacgacacg
    15421 ggtactgaaa gtattttaca agagttatta aaatgtcaat ctgaaaaatt tatcgcattt
    15481 ccatctatac gatttgaata ctttttagtc ttattgaaac atgctaaatt tatggtcggc
    15541 aactcaagtg caggtattag agaagctcct ctctacggtg tcccttcaat tgatgttggt
    15601 acacgccaaa gtaaccgcca tatgggaaaa tctattattc atacagatta tgaaactaaa
    15661 aatatctttg atgcgattca acaagcatgc agtttaggca aatttgaagc agatgatacc
    15721 tttaatggcg gagatactcg caccagcaca gaaagatttg ctgaagtaat caacaatcct
    15781 gaaacgtgga atgtttctgc tcaaaaacgt tttatcgatt tgaatcttta aattatggaa
    15841 aaacaaaata ttgcggttat acttgcgcgc caaaactcca aaggattgcc attaaaaaat
    15901 ctccggaaaa tgaatggcat atcattactt ggtcatacaa ttaatgctgc tatatcatca
    15961 aagtgttttg accgcataat tgtttcgact gatggcgggt taattgcaga agaagctaaa
    16021 aatttcggtg tcgaagtcgt cctacgccct gcagagctgg cctccgatac agccagctct
    16081 atttcaggtg taatacatgc tttagaaaca attggcagta attccggcac agtaacccta
    16141 ttacaaccaa ccagtccatt acgcacaggg gctcatattc gtgaagcttt ttctctattt
    16201 gatgagaaaa taaaaggatc cgttgtctct gcatgcccaa tggagcatca tccactaaaa
    16261 accctgcttc aaatcaataa tggcgaatat gcccccatgc gccatctaag cgatttggag
    16321 cagcctcgcc aacaattacc tcaggcattt aggcctaatg gtgcaattta cattaatgat
    16381 actgcttcac taattgcaaa taattgtttt tttatcgccc caaccaaact ttatattatg
    16441 tctcatcaag actctatcga tattgatact gagcttgatt tacaacaggc agaaaacatt
    16501 cttaatcaca aggaaagcta aatgcaaaac aacaacgaat ttaaaattgg taatcgttca
    16561 gtaggttaca accacgaacc attgattatc tgtgaaatcg gcatcaatca tgaaggctct
    16621 ttaaaaacag cttttgaaat ggttgatgct gcctataatg caggcgctga agttgttaaa
    16681 catcaaacac acatcgttga agacgaaatg tctgatgagg ccaaacaagt cattccaggc
    16741 aatgcagatg tctctattta tgaaattatg gaacgttgcg ccctgaatga agaagatgag
    16801 attaaattaa aagaatacgt agagagtaag ggtatgattt ttatcagtac tcctttctct
    16861 cgtgcagctg ctttacgatt acaacgtatg gatattccag catataaaat cggctctggc
    16921 gaatgtaata actacccatt aattaaactg gtggcctctt ttggtaagcc tattattctc
    16981 tctaccggca tgaattctat tgaaagcatc aaaaagtcgg tagaaattat tcgagaagca
    17041 ggggtacctt atgctttgct tcactgtacc aacatctacc caacccctta cgaagatgtt
    17101 cgattgggtg gtatgaacga tttatctgaa gcctttccag acgcaatcat tggcctgtct
    17161 gaccatacct tagataacta tgcttgctta ggagcagtag ctttaggcgg ttcgatttta
    17221 gagcgtcact ttactgaccg catggatcgc ccaggtccgg atattgtatg ctctatgaat
    17281 ccggatactt ttaaagagct caagcaaggc gctcatgctt taaaattggc acgcggcggc
    17341 aaaaaagaca cgattatcgc gggagaaaag ccaactaaag atttcgcctt tgcatctgtc
    17401 gtagcagata aagacattaa aaaaggagaa ctgttgtccg gagataacct atgggttaaa
    17461 cgcccaggca atggagactt cagcgtcaac gaatatgaaa cattatttgg taaggtcgct
    17521 gcttgcaata ttcgcaaagg tgctcaaatc aaaaaaactg atattagtta ataataaaat
    17581 agattaagct attcttaaat tcagaatatt gcttatctat attaaaaatt tctaattttt
    17641 aaggttctga ttgaaatcag aaccttattt caactattac tttttactca taatcgaatt
    17701 atatacttta ggactttata atatggctgt tattatattt gttaacggaa ttcgggctgt
    17761 aaatggcctt gttaaatcat ctatcaatac tgcaaacgct tttgctgaag aaggactgga
    17821 tgttcattta attaattttg ttggcaatat tactggagca gagcatttat accccccatt
    17881 ccacttacat cccaatgtca aaacctccag catcatagat ttatttaatg acattccaga
    17941 aaatgttagc tgccgaaata ctccttttta ttctattcat caacaattct tcaaagctga
    18001 atatagtgcc cactataagc atgttttgat gaaaattgaa tctttattat ctgcagaaga
    18061 tagcattatc ttcactcatc ctcttcaact ggaaatgtat cgtttagcga ataatgatat
    18121 caagtcaaaa gccaaactaa ttgtacaaat tcatggtaat tatatggaag aaatccataa
    18181 ctatgaaatt ttggcacgaa atatcgatta tgttgactat cttcaaacgg tatctgatga
    18241 aatgctggaa gaaatgcatt cccatttcaa aatcaaaaaa gacaaattag tttttattcc
    18301 aaacatcact tatcccattt cattagaaaa aaaagaagct gatttcttta ttaaggatga
    18361 tgaagacatc gataatgctc agaaatttaa acgtatctct attgttggca gcattcagcc
    18421 aagaaaaaac caattggatg ccattaaaat catcaataaa attaaaaatg aaaattacat
    18481 tttacagata tatggcaaat ctattaataa agattacttt gaattaatta aaaaatatat
    18541 taaagacaat aagttacaaa accgtatctt attcaaaggt gaatcttccg agcaggaaat
    18601 ttatgaaaat acagatatcc tgatcatgac atcagaaagt gagggatttc catatatatt
    18661 tatggaaggc atggtgtatg atattccaat cgttgtatat gattttaaat atggagcgaa
    18721 tgattacagt aactataatg aaaatggttg tgtttttaaa actggtgata tttctggaat
    18781 ggcaaaaaaa ataattgagc tattaaataa cccagaaaaa tataaagaat tagttcaata
    18841 taatcacaat cgcttcttaa aagaatatgc aaaagatgtg gttatggcta aatatttcac
    18901 tattcttccg cgcagcttta ataacgtatc attatcgtct gctttcagcc gaaaagaatt
    18961 ggacgaattc caaaatatta ctttttctat tgaagattct aatgatttag ctcatatttg
    19021 gaatttcgag ctaaccaatc ctgcacaaaa tatgaatttt tttgctttag ttggcaagcg
    19081 aaaatttcca atggatgctc atatccaagg aacacagtgt acgattaaga tagctcataa
    19141 aaagacaggg aatttattgt cgcttttact aaaaaaacga aatcagttga atttatcaag
    19201 gggatatacc ttaattgcag aagataatag ctatgaaaaa tatattggag caatatctaa
    19261 taaaggtaac tttgaaatta ttgcaaataa aaagagctca ttagttacta taaacaaaag
    19321 taccttagag ttgcatgaga ttccccatga actacatcag aataaattac tgattgcttt
    19381 acccaacatg caaacgcctc taaaaattac tgatgataat ttaataccta tccaagcctc
    19441 cataaaatta gaaaagattg gaaatactta ttacccatgt ttcttgccat ctggcatatt
    19501 taataatatc tgcttagatt acggtgaaga atccaaaatt attaatttta gtaaatattc
    19561 ttataaatat atctatgact caattcgtca tattgagcaa catacagata tatcggatat
    19621 tatcgtttgc aatgtttatt cttgggaact tattcgtgcc tcagttattg agagccttat
    19681 ggaatttacc ggaaaatggg aaaaacactt tcagacttct cctaaaattg attatcgatt
    19741 tgatcatgaa ggtaagcgtt cgatggatga tgtcttttca gaagaaacat ttattatgga
    19801 atttccgcgt aaaaatggta tagataagaa aacagcagcc ttccaaaata taccaaacag
    19861 tattgtaatg gagtatccgc agaccaatgg ttacagtatg cgcagtcatt cactgaaaag
    19921 taatgtagtt gcggcaaaac attttcttga aaaattaaat aaaattaagg tagatattaa
    19981 atttaaaaag catgaccttg caaacatcaa aaaaatgaat cgaattattt atgagcattt
    20041 aggcattaac ataaatatcg aagcatttct aaaaccacga ttagaaaaat ttaagcgtga
    20101 agaaaaatat tttcatgatt tcttcaaaag aaataatttt aaagaggtaa tttttccaag
    20161 cacttattgg aatccaggta ttatttgtgc tgcacataaa caaggtatta aggtatctga
    20221 tattcaatat gctgccatta ctccttatca tcctgcgtat tttaaatcac caaaatcaca
    20281 ttacgttgct gataaattgt tcttatggtc tgaatattgg aatcatgagc ttttaccaaa
    20341 tccaacacga gagattggtt ctggtgccgc atattggtat gcattagatg atgtgagatt
    20401 ttcagaaaaa ctgaattatg actatatctt tctatctcaa agtaggattt cttcgcgctt
    20461 gcttagtttt gcaattgagt ttgcattaaa aaatcctcaa ctacagcttt tattttctaa
    20521 gcatccagat gaaaatatag atttaaagaa cagaattatt cctgataatc ttataatctc
    20581 cacggaatct tctatacaag gcatcaatga atctcgcgtt gctgtaggtg tttattcaac
    20641 tagcttattt gaggcattag catgcggcaa acaaactttt gttgttaaat atccgggata
    20701 tgaaattatg tcaaatgaaa tagattcagg gttattcttt gcagtagaaa cacctgaaga
    20761 aatgcttgag aaaacaagcc cgaattgggt ggctgtggca gatattgaaa accagttttt
    20821 tggccaagaa aaataatttt taagttaatc tttaaaagtg caatattttt cttattttaa
    20881 atattgcact ttattctttt aatcatagga actcacatgt attctgaaca gggaattaat
    20941 aatactatta acatatctac tacatcttta accaatgcca cgcaactaac tgtgatcggg
    21001 aataataact ctgtatatat aggtaataat tgtaaaatcg tcagctc
//
LOCUS       DAWFBO010000020        20989 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFBO010000020 DAWFBO010000000
VERSION     DAWFBO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.020"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(88..861)
                     /locus_tag="WGO87_000512"
     CDS             complement(88..861)
                     /locus_tag="WGO87_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0391911.1"
                     /translation="MSVNILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
     gene            complement(960..1583)
                     /gene="rsmG"
                     /locus_tag="WGO87_000513"
     CDS             complement(960..1583)
                     /gene="rsmG"
                     /locus_tag="WGO87_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0391912.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQGEIDRLPQDVCVEKVQRLDVPGLDAERHIAILRKR"
     gene            complement(1679..1960)
                     /locus_tag="WGO87_000514"
     CDS             complement(1679..1960)
                     /locus_tag="WGO87_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391913.1"
                     /translation="MNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAACAAAAVAWIFE
                     LLLGNAALGGLRGEAVSEYAFAVWLVAMLTLPKHLTRAPVQPVVFHRKK"
     gene            complement(1957..2151)
                     /locus_tag="WGO87_000515"
     CDS             complement(1957..2151)
                     /locus_tag="WGO87_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HEZ0391914.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDAFALAFGRAGLLVSV
                     SAGVAVGTVLFGLCSVCLRF"
     gene            2258..2333
                     /locus_tag="WGO87_000516"
     tRNA            2258..2333
                     /locus_tag="WGO87_000516"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2291..2293,aa:Thr,seq:cgt)
     gene            2407..3534
                     /locus_tag="WGO87_000517"
     CDS             2407..3534
                     /locus_tag="WGO87_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0391915.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            3715..4272
                     /gene="frr"
                     /locus_tag="WGO87_000518"
     CDS             3715..4272
                     /gene="frr"
                     /locus_tag="WGO87_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0391916.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            4328..5074
                     /locus_tag="WGO87_000519"
     CDS             4328..5074
                     /locus_tag="WGO87_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0391917.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFSRQILKGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAKGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            5077..5874
                     /locus_tag="WGO87_000520"
     CDS             5077..5874
                     /locus_tag="WGO87_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0391918.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            5879..7114
                     /locus_tag="WGO87_000521"
     CDS             5879..7114
                     /locus_tag="WGO87_000521"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0391919.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSLHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            7149..8489
                     /gene="rseP"
                     /locus_tag="WGO87_000522"
     CDS             7149..8489
                     /gene="rseP"
                     /locus_tag="WGO87_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0391920.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            8546..10939
                     /gene="bamA"
                     /locus_tag="WGO87_000523"
     CDS             8546..10939
                     /gene="bamA"
                     /locus_tag="WGO87_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0391921.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGERFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            11005..11505
                     /locus_tag="WGO87_000524"
     CDS             11005..11505
                     /locus_tag="WGO87_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0391922.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVATFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            11538..12584
                     /gene="lpxD"
                     /locus_tag="WGO87_000525"
     CDS             11538..12584
                     /gene="lpxD"
                     /locus_tag="WGO87_000525"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0391923.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            12618..13067
                     /gene="fabZ"
                     /locus_tag="WGO87_000526"
     CDS             12618..13067
                     /gene="fabZ"
                     /locus_tag="WGO87_000526"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0391924.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            13154..13930
                     /gene="lpxA"
                     /locus_tag="WGO87_000527"
     CDS             13154..13930
                     /gene="lpxA"
                     /locus_tag="WGO87_000527"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0391925.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRHAETQAELAVFRDFFAQSARGIIR"
     gene            14288..15679
                     /locus_tag="WGO87_000528"
     CDS             14288..15679
                     /locus_tag="WGO87_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0391926.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPLMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFMPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMALA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAIVVLGGIKGIAKA
                     ASFIVPAMAVFYVVGGISIIVINSDALMPAVNLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            15758..17014
                     /locus_tag="WGO87_000529"
     CDS             15758..17014
                     /locus_tag="WGO87_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0391927.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTASRYQ
                     INKERMVRMSEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFGNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQALFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            17195..18004
                     /gene="zupT"
                     /locus_tag="WGO87_000530"
     CDS             17195..18004
                     /gene="zupT"
                     /locus_tag="WGO87_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0391928.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            18101..20938
                     /locus_tag="WGO87_000531"
     CDS             18101..20938
                     /locus_tag="WGO87_000531"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0391929.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLANNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPGFALPEKYANLDRFVARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGTVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPGMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEVALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESEDAPIAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNTKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
BASE COUNT         5063 a         6341 c         5387 g         4198 t
ORIGIN      
        1 acgatataag tatttttatt ttaatccgcc atattaacgc actcggccaa acagcataaa
       61 ggcacgggca gcccgatttg gattgaccta tttccccgac accctcgcca tcagctcgtc
      121 cgccaaggca agatacgcct tggtaccctt tgcctgcgcg tcgtaagcca taaccggcat
      181 accgtggctc ggggcttccg caaggcggat attgcgcggg atgacggttt caaaaagcaa
      241 atccccgaaa tggctgcgca actgttcgct gacttcggca accagcctgc tgcggctgtc
      301 gtacatcgtg cgcacgatgc ccgtgatgtc caaatcggga ttgaccgcct gacggatttt
      361 gcgcacggtc gcaatcaaat cggaaatccc ctcaagcgcg taatattcgc acaacatcgg
      421 cacaatcacg ccgcctgccg ccaccaagcc gttaagcgtc aacagcgtca gcgaaggcgg
      481 gcagtcgatc aggataaagt cgtaatcttc cgccactgcc ttgagcgcgt ttttcaaacg
      541 cacttcccgg gcgatttcct gtaccaactc gatttccgca ccggccagcg cgcggttcgc
      601 gcccaacaca gcgtatccgc cctctttgct gcgtaccgcc gccgactgca cgtccgcatc
      661 gcccaataag acctgataaa cgccggactg caaactcgcc ttgtcgatgc cgctgcccgt
      721 cgtcgcattg ccctgcggat ccaaatcgac caccagcacg cgtttgccgc gcgatgccag
      781 cgaagccgcc aaatttaccg tcgtcgtcgt tttgcccaca ccgcccttct gattggcgat
      841 ggcaaggatg ttcacactca tgtcccgccc gtgtcccgtc tgatgttgaa aaaagaaaaa
      901 cagtgcggaa ttttaccttt atccgcacaa aaaaggtatt catgccgtct gaagcgcgct
      961 caacgcttgc ggagtatggc gatatggcgt tccgcatcca agcccggcac gtcaagcctt
     1021 tggacttttt cgacgcacac atcctgcggc aggcggtcga tttccccctg cggatacacg
     1081 cccttcatcg ccgcccagta tccgccgtct ttcaacagat gcaccgtcca cgacacaaaa
     1141 tccgccagtt ctgcaaacgc acggctggta accacgtcgg cacgcacgtc cgaaaccgcc
     1201 tccacgcgtc cggataccac gcgcacattg tccaatccca actcgataac cgcctgctgt
     1261 aaaaaagccg ttttcttcgt attcgcatcc aaaagggtta tctgcacatc cggacggcac
     1321 accgccgccg gaatgccggg ctgaccgccg cccgaaccga catccagcat cgtttgcaca
     1381 ccctcgatat ggggcagcag cgtcaggctg tccaaaagat gatggacaat cattttttcc
     1441 tcgtcgcgca gggcggtcag attgtaggtt ttgttccact ttttcaacaa atccacatag
     1501 gccaaaagcc tgtcctgcgc cgtttccgaa atatccagcc ccatcgcggc aatgcctgca
     1561 cgcaggcgtt ctttgcgttc catatctgct tcctgtccat attcaggcgt aatgttaacg
     1621 gaaaaacatg ccgtctgaaa ccgcgtgtcg ggtttcagac ggcatccccc gttccgtcct
     1681 attttttcct gtgaaacacc accggctgca ccggcgcgcg cgtcaggtgt ttgggcagcg
     1741 tcagcatcgc caccagccac acggcaaacg catattccga caccgcctcc ccgcgcagcc
     1801 cgcccaaggc ggcattgccc aacaacagct cgaaaatcca agccaccgct gctgccgcgc
     1861 aagccgccac gcccgacaga aacagcgcgt aacgcgaatg ccgccgcatc atctttttgt
     1921 acgccagctt cgtcaggcct gccgacacga ccaggttcaa aaacgcaaac acaccgaaca
     1981 caaaccaaac agtaccgtcc caaccgccac gccggccgac accgacacca gcagccctgc
     2041 ccgtccgaac gcaagcgcaa aggcatcatg caccgccaac gcaaacggca acgtcaaaaa
     2101 caccagcccc gcaaacagag ccgcataagg atctttctcg ttcacaaaca tcgccgtccc
     2161 cctcttccga agcagaccgc attataccgc gcactgcctt gccgcccgcc agaaaagttg
     2221 cacaaacaac cgttcatata tatcataacg aaaaaacgcc ggtgtagctc agtcggtaga
     2281 gcagcgcatt cgtaacgcga aggtcggggg ttcgattccc ttctccggca ccaataccaa
     2341 gcacagaccc tcccttcctc gggaagcctg tgctttttca catttccgct tcagacggca
     2401 accgatatga acacctcgca acgcaaccgc ctcgtcagcc gctggctcaa ctcctacgaa
     2461 cgctaccgcc accgccgcct catacacgcc gtgcggctcg gcggggccgt cctgttcgcc
     2521 accgcactcg cccggctgct ccacctccaa cacggcgaat ggataggcat gaccgtcttc
     2581 gtcgtcctcg gcatgctcca gtttcaaggg gcgatttact ccaaggcggt ggaacgtatg
     2641 ctcggcacgg tcatcgggct gggcgcgggt ttgggcgttt tatggctgaa ccagcattat
     2701 ttccacggca acctcctctt ctacctcacc gtcggcacgg caagcgcact ggccggctgg
     2761 gcggcggtcg gcaaaaacgg ctacgtccct atgctggcgg ggctgacgat gtgcatgctc
     2821 atcggcgaca acggcagcga atggttcgac agcggcctga tgcgcgccat gaacgtcctc
     2881 atcggcgcgg ccatcgccat tgccgccgcc aaactgctgc cgctgaaatc cacactgatg
     2941 tggcgtttca tgcttgccga caacctgacc gactgcagca aaatgattgc cgaaatcagc
     3001 aacggcaggc gtatgacgcg cgaacgtttg gagcagaata tggttaaaat gcgccaaatc
     3061 aacgcacgca tggtcaaaag ccgcagccac ctcgccgcca catcgggcga aagccgcatc
     3121 agccccgcca tgatggaagc catgcagcac gcccaccgta aaattgtcaa caccaccgag
     3181 ctgctcctga ccactgccgc caagctgcaa tctcccaaac tcaacggcag cgaaatccgg
     3241 ctgctcgacc gccacttcac actgctccaa accgacctgc aacaaaccgt cgcccttatc
     3301 aacggcagac acgcccgccg catccgcatc gacaccgcca tcaaccccga actggaagcc
     3361 ctcgccgaac acctccacta ccaatggcag ggcttcctct ggctcagcac caatatgcgt
     3421 caggaaattt ccgccctcgt catcctgctg caacgcaccc gccgcaaatg gctggatgcc
     3481 cacgaacgcc aacacctgcg ccaaagcctg cttgaaacac gggaacacgg ctgacggtcg
     3541 gacacgatgc cgtctgaaac gcctgccgct tcagacggca tctccaatcc tcctgccttg
     3601 tcgtcatgcc cgaataacgg tactattcgc agttaacagt tttcatgccg tctgaaaggt
     3661 ttgaagcgcg tttcagacgg ctgcccgccc accttatccc accgaaagaa caatatgatt
     3721 aacgatattc aaaaaacagc cgaaggcaaa atgcagcgtt cggtcgaagt actgaaagaa
     3781 aatctggcga aagtgcgtac cggccgcgcg cataccggtc tgctcgacca agtggaagtc
     3841 gaatactggg gcagcatggt tcccgtcagc caagttgcca acgtaacgct tctggacgcg
     3901 cgcaccatcg gcgtgaaacc gtttgagggc aatatggcgg ccaaagtcga gaaagccatc
     3961 cgcgattcaa acttgggact gaacccggca gctgtcggcg atctgatccg cgtgccgatg
     4021 cccatgctga ccgaggaacg ccgcaaagac ctgattaaag tcgtacgcgg cgaagcggaa
     4081 gaaggccgcg tctctatccg caacgtgcgc cgcgatgcca acgaccacat caaaaaactc
     4141 ctcaaagaca aagaaatttc cgaagacgag gcacgtcgcg gcgaagaagc ggttcaaaaa
     4201 ctgaccgaca aatacattac cgaagccgac aaactcctga ctgccaaaga agaagatttg
     4261 atggcaattt aacctgcacg gttcggcgtt cagacggcat ttgaatgccg aaccgcgaaa
     4321 ggcagacatg aaaagcagca cgcaggccgt tttggaacac accgccattc ccaagcatat
     4381 cgccgtgatt atggacggca acggccgttg ggcgaaaaaa cgtttcctcc cgcgcataat
     4441 gggacacaaa cgcggtttgg acgcattgga aaatatggtg aagcattgcg ccaaactggg
     4501 tgtgcaatat ctgaccgtgt ttgccttttc aaccgaaaac tggcgccgcc ccgaagacga
     4561 agtttcgttc ctgatggggc tgtttttaca ggctttgcaa aaacaggtac gccgcctgca
     4621 cgaaaacaat atgcgcctga aaatactggg cagccgcgaa cgcttcagcc gtcaaatcct
     4681 gaagggcatc gaagaggcgg aggcgttgac ggcaaacaat accggtctga ccctgagcat
     4741 tgccgccgat tacggcggca gatgggacat tttgcaggcg gcaaacaaac tgattgccaa
     4801 aggcgtatcc gagattacgg aagacacgct ggcgaaacac ttgatgctgg gcgatgcgcc
     4861 cgagccggat ttgttcatcc gcaccggcgg cgaaacgcgc atcagcaatt tcctactctg
     4921 gcaaatggct tatgccgaac tgtatttcac cgatattttg tggcccgatt ttgacggcaa
     4981 ggctttggac gatgccgtcg cttcgttcca aaaacgcgaa cggcggttcg gacgcacctc
     5041 cgagcaactg cctatcgaac agcaaaggaa ctgaatatgc tgaaacaacg ggtaataacc
     5101 gccatgtggc tgctgccgct gatgctgggc atgctgtttt acgcgccgca atggttgtgg
     5161 gctgcatttt gcggactgat tgccctgatt gccttgtggg aatatgcccg tatgggcggt
     5221 ttgtgcaaaa ttaaaaccaa ccattacctc gccgcaacct tggttttcgg cgtggttgcc
     5281 tatgcgggcg gctggatgct gcctaatttg gtttggtatg ttgttttggc attttggctc
     5341 gccgttatgc ctttatggtt gagattcaaa tggaggctca acggcggttg gcaggtttat
     5401 gccgtcggct ggcttctggt catgccgttt tggttcgcgc tcgtatccct gcgcccgcat
     5461 cccgatgatg ccctgccgct gctcgccgtg atgggtttgg tgtgggttgc cgatatttgc
     5521 gcgtatttca gcggcaaggc gttcggcaaa cacaaaatcg caccggcaat cagccccggc
     5581 aaaagctggg aaggtgcaat cggcggcgcg gtttgcgtgg cagtgtacat gaccgccgta
     5641 cgaagtgccg gctggctggc attcgataca ggctggttcg ataccgtgtt aatcggtttg
     5701 gtgctgaccg ttgtcagcgt atgcggcgac ctgttggaaa gctggctcaa gcgcgcggcg
     5761 ggcattaaag acagcagcaa cctgctgtcc ggacacggcg gcgtgttcga ccgtaccgac
     5821 agcctgattg ccgttatcag cgtctatgcg gcgatgatgt cggttttaaa ttgattctat
     5881 gccgtctgaa aacgcttcag acggcatccg gtataaagtt atccccatta tgacaccaca
     5941 agtcctgacc atattaggca gtaccggcag cataggcgaa agcacgctgg acgttgtctc
     6001 cctccacccc gaaaaattcc gcgtattcgc gctggcaggg cataagcagg tcgagaaact
     6061 ggcggtgcaa tgccggacgt tccgccccga atatgccgtc gttgccgatg ccgaacacgc
     6121 cgcccggctt gaagctttgt tgaaaggcga cggcacggcg acgcaggttt tacacggcgc
     6181 gcaggcattg gttgacgttg cctctgccga cgaagtcagc ggtgtcatgt gcgccatcgt
     6241 cggggcggtg gggctgcctt ccgcgctcgc agcggcgcaa aaaggcaaaa ccatttatct
     6301 ggcgaacaaa gagactctgg tggtttccgg cgcgttgttt atggaaaccg cccgtgcaaa
     6361 cggcgcggca gtgctgcccg tcgacagcga acacaacgcc gttttccaag ttttgccgcg
     6421 cgattacgcc ggccgtctga acgaacacgg catcgcttcg attatcctga ccgcttccgg
     6481 cggcccgttt ctgaccgccg atttaaacac gttcgacagc attacgcccg accaagcggt
     6541 caaacacccc aattggcgta tgggacgcaa aatctccgtc gattccgcca ccatgatgaa
     6601 caaaggtttg gagctgattg aagcgcattg gctgttcaac tgtccgcccg acaaactcga
     6661 agtcgtcatc catccgcaat ccgtgataca cagcatggtg cgctaccgcg acggctccgt
     6721 gttggcgcaa ctgggcaatc ccgatatgcg aacgcccatc gcttattgtt tgggtttgcc
     6781 cgagcgcatc gattcgggtg tcggcgacct ggatttcgac gcattgtccg cgctgacctt
     6841 ccaaaagccc gactttgacc gcttcccctg cctgaagctc gcctatgaag ccatgaacgc
     6901 aggcggagcc gcgccctgcg tattgaacgc cgccaacgaa gccgccgtcg ccgccttttt
     6961 ggacgggcag attaagttta ccgacattgc caaaaccgtc gcccattgtc tcgcacaaga
     7021 cttttcagac ggcataggcg acataggggg gctcttggcg caagatgccc ggacacgcgc
     7081 acaagcgcgg gcatttatcg gcacactgcg ctgatgccgt ccgaacaccg ttatcaaagg
     7141 aaaaccattt gcacaccctt ctagctttta tcttcgccat cctgattttg gtcagcctgc
     7201 acgagttcgg acactacatc gttgccagat tgtgcggcgt caaagtcgta cgcttttccg
     7261 tcggcttcgg caaaccgttt ttcacccgaa agcgcggcga caccgaatgg tgcctcgccc
     7321 cgattccgtt gggcggttac gtcaaaatgg tcgatacgcg cgaaggcgaa gtatcagaag
     7381 ccgatttacc ctacgctttt gacaaacaac accccgccaa gcgcatcgcc atcgtcgccg
     7441 ccggcccact gaccaacctc gcactggcgg ttttgctgta cggactgagc ttttccttcg
     7501 gcgtaaccga actgcgcccc tacgtcggca cagtcgaacc cgacaccatt gccgcccgcg
     7561 ccggcttcca aagcggcgac aaaatacaat ccgtcaacgg cacacccgtt gcagattggg
     7621 gcagcgcgca aaccgaaatc gtcctcaacc tcgaagccgg caaagtcgcc gtcggcgttc
     7681 agacggcatc gggcgcgcaa accgtccgca ccatcgatgc cgcaggcacg ccggaagccg
     7741 gtaaaatcgc aaaaaaccaa ggctacatcg gactgatgcc ctttaaaatc acaaccgttg
     7801 ccggcggcgt ggaaaaaggc agccccgccg aaaaagcagg cctgaaaccg ggcgacaggc
     7861 tgactgccgc cgacggcaaa cccatcgcct catggcaaga atgggcaaac ctgacccgcc
     7921 aaagccccgg caaaaaaatc accctgaact acgaacgcgc cggacaaacc cataccgccg
     7981 acatccgccc cgatactgtc gaacagtccg accacaccct gatcgggcgc gtcggcctcc
     8041 gtccgcagcc ggacagggcg tgggacgcgc aaatccgccg cagctaccgt ccgtctgtta
     8101 tccgcgcatt cggcatgggc tgggaaaaaa ccgtttccca ctcgtggaca accctcaaat
     8161 ttttcggcaa actaatcagc ggcaacgcct ccgtcagcca tatttccggg ccgctgacca
     8221 ttgccgacat tgccggacag tccgccgaac tcggcttgca aagttatttg gaatttttgg
     8281 cactggtcag catcagcctc ggcgtgctga acctactgcc cgtccccgtt ttggacggcg
     8341 gccacctcgt gttttatact gccgaatgga tacgcggcaa acctttgggc gaacgcgtcc
     8401 aaaacatcgg tttgcgcttc gggcttgccc tcatgatgct gatgatggcg gtcgccttct
     8461 tcaacgacgt tacccggctg ctcggttaga ttttacgttt cggaatgccg tctgaaaccg
     8521 cattccgcac cacaaggaac tgacaatgaa actgaaacag attgcttccg cactgatggt
     8581 cttgggcata tcgcctttgg cacttgccga cttcaccatc caagacatcc gcgtcgaagg
     8641 cttgcagcgt accgagccga gtaccgtatt caactacctg cccgtcaaag tcggcgacac
     8701 ctacaacgac acacacggca gtgccatcat caaaagcctg tacgccaccg gtttctttga
     8761 cgacgtacgc gtcgagactg cggacgggca gctcctgctg accgtcatcg aacgccccac
     8821 catcggctcg ctcaacatca ccggcgcaaa aatgctgcaa aacgacgcca ttaagaaaaa
     8881 tctcgaatcg ttcgggctgg cgcagtcgca atactttaat caggcgacac tcaatcaggc
     8941 agtcgccggc ctgaaagaag aatacctcgg gcgcggcaaa ctcaatatcc aaatcacgcc
     9001 caaagtaacc aaactcgccc gcaaccgcgt cgacatcgac atcacgattg acgagggcaa
     9061 atccgccaaa atcaccgaca tcgaatttga aggcaaccaa gtctattccg accgcaaact
     9121 gatgcggcag atgtcgctga ccgaaggcgg catttggaca tggctgacac gaagcaacca
     9181 attcaacgag cagaaatttg cccaagacat ggaaaaagta accgacttct accaaaacaa
     9241 cggctacttc gatttccgca tcctcgatac cgacatccaa accaacgaag acaaaaccaa
     9301 gcagaccatc aaaatcaccg tccacgaagg cgaacgtttc cgttggggca aagtctccat
     9361 cgaaggcgac accaacgaag tccccaaagc cgaactggaa aaactgctga ccatgaagcc
     9421 cggcaaatgg tacgaacgcc agcagatgac cgccgttttg ggtgagattc agaaccgcat
     9481 gggctcggca ggctacgcat acagcgaaat cagcgtacag ccgctgccaa acgccgaaac
     9541 caaaaccgtc gatttcgtcc tgcacatcga accgggccgg aaaatctacg tcaacgaaat
     9601 ccacatcacc ggcaacaaca aaacccgcga cgaagtcgtg cgccgcgaat tgcgccaaat
     9661 ggaatccgcg ccttacgaca cctccaagct gcaacgctcc aaagagcgcg tcgagctttt
     9721 gggctacttc gacaacgtac agtttgatgc cgtcccgctt gccggcacac ccgacaaagt
     9781 cgatttgaac atgagcctga ccgaacgttc caccggctcg ctcgacttga gcgcgggctg
     9841 ggtacaggat accggcctgg tcatgtccgc aggcgtttcc caagacaacc tgttcggtac
     9901 gggcaagtcg gccgccctgc gcgcctcacg aagcaaaacc acgctcaacg gctcgctgtc
     9961 gtttaccgac ccgtacttca cggcagacgg ggtcagcctg ggctacgatg tttacggaaa
    10021 agccttcgac ccgcgcaaag catcgaccag catcaaacaa tataaaacca ccacggcagg
    10081 cgcaggcatc cgcatgagcg tgcctgttac cgaatacgac cgcgtgaatt tcggtttggt
    10141 ggcagaacac ctgaccgtca acacctacaa caaagcgccc aaacactatg ccgactttat
    10201 caagaaatac ggcaaaaccg acggcacaga cggcagcttc aaaggctggc tgtacaaagg
    10261 taccgtcggc tgggggcgca acaaaaccga cagcgcgtta tggccgacgc gcggctacct
    10321 gacgggcgtg aacgccgaaa tcgccctgcc cggcagcaaa ctgcaatact actccgccac
    10381 ccacaaccaa acctggttct tccccctgag caaaaccttc acactgatgc ttggcggcga
    10441 aatcggctat gccaacggct acggcaaaac caaagaaatc cccttctttg aaaacttcta
    10501 cggcggcggc ctgggttcgg tacgaggtta cgaaagcggc acactcggtc cgaaagtcta
    10561 tgacgaatac ggcgaaaaaa tcagctacgg cggcaacaaa aaagccaacg tctccgccga
    10621 gctgctcttc ccgatgcccg gcgcgaaaga cgcgcgcacc gtccgcctga gcctgtttgc
    10681 cgacgcaggc agcgtgtggg acggcaaaac ctacgacgac aacagcagtt ccgcgaccgg
    10741 cggcagggtt caaaacattt acggcgccgg caatacccat aaatccacct ttaccaacga
    10801 attgcgctat tccgccggcg gcgcggttac ctggctctcg cctttaggcc cgatgaaatt
    10861 cagctacgcc tacccgctga agaaaaaacc ggaagacgaa atccaacgct tccaattcca
    10921 actcggcacg acgttctaat cccgcaaatg ccgtctgaag cccttcagac ggcatttcgc
    10981 ggcaacattc gaaggagttt taccatgacc cgtttgaccc gcgcgtttgc cgcggctctg
    11041 atcggtttgt gctgcaccgc aggcgcgcac gccgacacct tccaaaaaat cggctttatc
    11101 aacaccgagc gcatctacct cgaatccaag caggcgcgca agattcaaaa aacgctggac
    11161 agcgaatttt ccgcccgtca ggacgaattg caaaaactgc aacgcgaagg tctggatttg
    11221 gaaaggcagc ttgccgaagg caaactcaga aacgcaaaaa aggcgcaagc cgaagaaaaa
    11281 tggcgcgggc tggtcgcaac gttccgcaaa aaacaggcgc agtttgaaga agactacaac
    11341 ctccgccgca acgaagagtt tgcctccctc cagcaaaacg ccaaccgtgt catcgtcaaa
    11401 atcgccaaac aggaaggtta cgatgtcatt ttgcagaacg tgatttacgt caacacccaa
    11461 tacgacgtta ccgacagcgt cattaaagaa atgaacgccc gctgaccctt tcagacggca
    11521 taccgaacag gaaaaccatg attccggcca cctacaccct gtcccaaatc accgcgcggc
    11581 tcggcggcga atggcgcggc gaggacattt ccgttaccgc cgtgcgcccg ctcgcagacg
    11641 cgcaggcgga acacatcagc ttccttgcca atccgaaata caaagccgaa gtccacgaca
    11701 gcagcgcggg cgcggtcatc gtttccgcca aagcggcaga cggatttgaa gggcgcaacc
    11761 tgattgtcgc cgacgacccc tatctctatt tcgccaaagt cgcccgcctg ttttcacccg
    11821 tcgtcaaagc gcgcggcggc atccatccga ccgccgtcgt cgaaccgggc gcgaccgttc
    11881 ccgccagctg cgaaatcggc gcgaacgtct acatcggcgc aaacaccgtg ctcggcgaag
    11941 gctgccgcat cttggcaaac gccgtcgtcc aacacgattg caaactgggc gacgaagtcg
    12001 tcctgcatcc caacgccgtc gtttattacg gctgcacact gggcagacgc gtcgaaatcc
    12061 acagcggcgc ggtcatcggc gcggacggtt tcggactcgc ttttgccggc gattcatggt
    12121 ttaaaatccc gcaaaccggc gcggtaacgc tgggcgacga cgtagaaatc ggctcgaaca
    12181 ccaacatcga ccgcggcgcg atgagcgaca ccaccgtcgg caacggcacc aaaatcgaca
    12241 accaagtcca aatcggacac aactgcaaaa tcggttcgca caccgtcatc gccgccaaaa
    12301 ccggcatctc aggcagcgta accataggca gctactgcat catcggcggc ggcgtcggta
    12361 cggtcggaca catcgaaatc gccgacaaaa ccaccatcgg cggcggcacg tccgtcaccc
    12421 acagcattac cgaaagcggc aaacacctcg ccggcatctt cccgatgtcc acccataaag
    12481 aatgggcgcg caacgctgtt tacatccacc gcttaagcga aatgaacaaa cggctcaaaa
    12541 cactggagca gcagctttca gatgccggtc aagacagcaa ataaccaaac cgactttatt
    12601 caaggaatac gacagacatg gacgtacaac tccccatcga agccaaagac atccaaaaac
    12661 tcatccccca ccgctatccg tttctccagc tcgaccgcat caccgccttc gagccgatga
    12721 aaaccctgac cgcgattaaa aacgtcacca taaacgaacc ccagttccaa ggccatttcc
    12781 ccgacctgcc cgtgatgccc ggcgtactca tcatcgaagc gatggcgcag gcgtgcggca
    12841 cgttggcgat tttgagcgaa ggcgggcgca aagaaaacga attcttcttc ttcgccggca
    12901 tagacgaagc ccgtttcaaa cgccaagtca tccccggcga ccaactcgtc tttgaagtcg
    12961 agctgctgac cagccggcgc ggcatcggca aattcaacgc cgttgccaaa gtagacgggc
    13021 aagtcgccgt cgaagccgtg attatgtgcg ccaaacgcgt ggtttgagtg ttcagaaaaa
    13081 ggtcgtctga aagttttcag acgacctgtt gccgtcgcgc atcttcgcgg caacacgaca
    13141 ggaaaggaaa aacatgaccc tcatccaccc gaccgccgtc atcgacccca aagccgaact
    13201 cgactccagc gtcaaagtcg gcgcgtacac cgtcatcggc cccaacgtcc aaatcggcgc
    13261 gaataccgaa atcggcccgc acgccgtcat caacggccac accagcatcg gcgaaaacaa
    13321 ccgcattttc caatttgcca gcctcggcga aatcccgcag gacaaaaaat accgcgacga
    13381 gccgaccaag ctgattatcg gcaacggcaa caccatccgc gaattcacca cctttaattt
    13441 aggcacggta accggcatcg gcgaaacccg tatcggcgac gacaactgga ttatggcgta
    13501 ctgccacctc gcgcacgact gcgtggtcgg caaccacacc atcttcgcca acaacgcctc
    13561 gcttgccgga cacgttacca tcggcgacta cgtcgttttg ggcggctaca cgctggtttt
    13621 ccaattctgc cgcatcggcg actacgccat gaccgcgttt gccgcaggcg tacacaaaga
    13681 cgtgccgccc tactttatgg catcgggcta ccgcgccgaa ccggcggggc tcaacagcga
    13741 aggcatgcgc cgcaacggtt ttaccgcaga gcagatttcc gccgtcaaag acgtgtacaa
    13801 aaccctctac catcgcggca ttccgtttga agaagccaag gcagacattc tccgccacgc
    13861 cgaaacccaa gccgagcttg ccgtattccg agactttttc gcacaatcgg cacgcggcat
    13921 catccgctga ccgtaccctt ttgatgccgt ctgaaacccc gacagcgttt cagacggcat
    13981 cggttttttt aaagcggtat attgtttgat cgatgcatca ttttgtagat ttcagaagta
    14041 tggcagtcgg cagcattgat gatttctgcc gcccgccaca tccctattcg gacatttcat
    14101 tgtgacctcc gccgctgctt ttaaagaatt gcaaacaatt tccgcagctt gaaccggcct
    14161 gttttcggcg aaaaaaacca aaagtatttt tcaaaagaac atattgtctt attggatatt
    14221 ctgtgctgaa attactggac actacaaaag tggtgtttta aaaataaaac agacaaagga
    14281 gaaagccatg caagtgtttt tagacaatcc caaagccttt tttgaaaccg tcagcggctg
    14341 ggtttgggga cctttgatgc tgatgctgct ggtcggcacg ggtattttgc tgactgtttt
    14401 attaaaaggt ttgcagttca cgatgttggg ttatgcgctg aaacaggcgt ttatgccgcc
    14461 aaagaagcat aaaagcggcg aaggccacga aggcgatatt tcccatttcg cggcgttgat
    14521 gaccgcgctg tccgccacca tcggtacggg taacatcgcc ggcgtggcga ctgcggtggt
    14581 aaccggcggc ccgggcgcgg tattttggat gtggatgacc gccattttcg gcatggccac
    14641 caaatacggc gaaggcgtgt tggcggtgaa ataccgcgtc aacaattcca aaggcgaaat
    14701 gtccggcggc ccgatgtatt acatcgaaaa aggcttgggc aaaaactgga aatggatggc
    14761 gttggcgttc gctttattcg gcacattcgc ttccttcggt atcggcagct cggtgcagtc
    14821 caactcggtt gcacaggcgg tgcaaaccag cttcggtatc gaacctgcct ataccggcat
    14881 tacgttgacc gttctgactg ccatcgttgt gttgggcggt atcaaaggca tcgccaaagc
    14941 cgcttctttc atcgtacctg ccatggcagt gttttatgtg gtaggcggta tctccattat
    15001 cgtgattaat tctgatgcgc tgatgcctgc cgtcaacctg attttctccg atgcgttcag
    15061 cgcgcaggcc gtggcaggtg gtgctatcgg taccgttatc cgctacggcg tggcgcgcgg
    15121 cgtgttctcc aatgaggcgg gcatgggttc tgcgccgatt gccgccgcag ccgccaaaac
    15181 cgaccatccc gtccgtcagg ccttggtttc tatgaccggt acgtttttgg ataccatcgt
    15241 cgtttgctcg attaccggca tcgtgttggt aatgggactg ctcggcgcgg gcggcgagtt
    15301 tgtcaaacct gaagtgagcg gcgcggcact gacaaccgtc acattccaaa aaatgctgcc
    15361 cggcatcggc ggctggattg tcaccatcgg cctgattttc tttgcctact caaccattct
    15421 cggctggtgc tattacggcg aaaaatgcgc ggtgtatgtt ttcggcgaga aatttgccgg
    15481 tttgtaccgc gtcggttatg tttcttccgt tatgctgggt accgtgttga gccttgattt
    15541 ggtgtggctg gcttcggaca cattcaacgg cttgatggca ttgcccaacc tgattgcact
    15601 tttattgatg gcgaaagtca tcgtcaatga aacgcgcgac ttcaaacaaa aaatcacaaa
    15661 cggcgaattg ccgcattaat atccgaggcc gtctgaatcc gaatattcag acggcacggc
    15721 atattaagtc tgttttgaaa aagccggagg gagaaacatg aaagtgcttg ttttaggtgc
    15781 gggtgttgcc ggcgtgtctt ccgcgtggta tctggcagag gccggacatg aagtaacggt
    15841 catcgaccgc gccgagggtg tggcgatgga aaccagtttt gccaacgcag gccagctttc
    15901 ttacggctat accacgcctt gggctgcacc cggtattccg accaaagcgc tgaaatggct
    15961 gtttaaaagc catccgcctt tgctgtttcg ccccgacggc agcctgtatc aaatcgaatg
    16021 gctgtggcgg atgctgcaaa actgcacggc atcccgctat caaatcaata aagagcgcat
    16081 ggtcaggatg tccgaataca gccgtgaaat gttccgccgt tttgaagcgc aaaccggcat
    16141 gaattttgag ggacgcaaaa aagggacgtt gcagattttc cgtcaggcta aagaagttga
    16201 agcggcagaa caagacattg ccgttttgga acgctacggc gtgccgtacc gccgtctgaa
    16261 gcccgaagaa tgcgcagaat tcgagcctgc gctggcacgc gttaccgcca aaattgccgg
    16321 cggcctgcac ctgcctgcgg atgcaaccgg cgactgccgc ctcttcactg aaaacctata
    16381 caaattgtgt caggaaaagg gcgtacggtt tcatttcaac caaaccatca gccgcatcga
    16441 ccacaacggg ctgcgcatca aaaccgttga aaccgaaaca gggcggtttg aagcagatgc
    16501 cgttgtctgc gcgctcggtt gtttcagcag gacggttttg gcgcagttgg atctcaatct
    16561 gcccatttat cccgtcaaag gctattcctt gaccctgccg gtcacaaatt ccgacggcgc
    16621 accggtgtcc accgttttag atgaaagcta caaagtcgcc atcacgcgct ttggcaacag
    16681 aatccgcgtc ggcggtatgg cggaattgtc gggctacgaa atcaaactgc ccgaaaaacg
    16741 ccgcgaaacc ttggctttgg tcgtcaacga tttgttcccg gaaggcggcg atttgagcca
    16801 agcattgttc tggagcggcc tcagaccgat gacgcccgac agcacgccgt taatcggccg
    16861 cacccgcttt gaaaacttat tcttgaatac aggccacggc actttgggct ggaccatgtc
    16921 gctgggttcg gcaaaattaa ccgccgatat cgtgagcggc aaagacaccg aaatccgaag
    16981 cgacgatttg agcctgtcgc gctatcaggc ttaagctgga aagcgataag gtttagcgac
    17041 atttcaaatt taaaggccgt ctgaaaatca tttttcagac ggccttttgt ttcaaaattc
    17101 gggtatccaa tcaataaaat aaataataac tattatcata cgcattaaaa agtgttagtc
    17161 tgaacctgtc ccagctttta gcgcaggttt tcccatgccc gatttttcga tgtccaattt
    17221 ggccgttgcc ttttccatta cgttggctgc cggtttgttt accgtattag gcagcggctt
    17281 ggtgatgttt tccaaaacgc ccaatccgcg cgtgttgtcg tttggtttgg catttgccgg
    17341 cggtgcgatg gtgtatgttt ccctgacgga gattttcagt aagtccagcg aggcgttcgc
    17401 tgaaatttat gataaagacc acgcgtttgc ggcggcgacc atggcatttt tggccgggat
    17461 gggcggcatt gcgctgattg accgtctggt gccgaacccg catgaaactt tagacgcgca
    17521 agacccgtcg tttcaagaaa gcaaacgccg ccatatcgcg cgagtcggca tgatggcggc
    17581 gtttgcgatt actgcgcaca atttccccga aggcttggcg acgttttttg ccacattgga
    17641 aaatccagca gtcgggatgc ctttggcctt ggcgattgcc atccataata ttccggaggg
    17701 catttccatc gccgcgccgg tttattttgc cacccgcagc cgtaagaaaa cggtgtgggc
    17761 gtgtctgcta tccggcttgg ccgagccgtt gggggcggct ttgggctatt tggttttgca
    17821 gccgtttttg tcgcctgccg tgtttggttc ggtattcggc gtgatagccg gtgtgatggt
    17881 gtttttggcg ttggacgagc tgctgccggc tgccaaacgc tattcagacg gccatgaaac
    17941 cgtttacggc ctgacaatgg gcatggcggt gattgccgtc agcctggtat tgttccattt
    18001 ttaaaacctg tatgccaaag gccgtctgaa atgattcaga cggccttttt gcgttataat
    18061 ccgcacatca aaaataacct taatttcaca aagttccatc atgttagaca aatacaatcc
    18121 agccgaaatc gaatccaaac attatcaaaa ctgggaagag cagggctatt tccaacctga
    18181 tatggatttg accaaaccgt ctttctccat ccaactgccg ccgcccaacg taaccggcac
    18241 gctgcacatg ggccatgcct tcaaccaaac cattatggac ggcctgaccc gctactaccg
    18301 catgaaaggc tgcaacaccg cctggattcc cggcaccgac cacgcgggca tcgccacgca
    18361 aatcgtggtc gagcgtcagc ttgccgcgca aaacgtgtcc cgtcatgact tgggccgcga
    18421 aaaattcttg gaaaaagtgt gggaatggaa agaagtgtcc ggcggcacga ttacccaaca
    18481 aatgcgccgc gtgggctgct ctgccgactg gacgcgcgag tatttcacga tggacgacgt
    18541 gcgcgccgaa accgtgacag aagtgttcgt acgcctgtat gaacagggct tgatttatcg
    18601 cggcaaacgc ttggtaaact gggatcccgt gcttggcacg gcggtatcgg atttggaagt
    18661 ggaaagcgtg gaagaacaag gctctatgtg gcacatccgc tatccgctgg ccaacaatcc
    18721 tgccgaagcc gttatcgtgg cgaccacccg ccctgaaacg ctgctgggcg acgtggccgt
    18781 tgccgtcaat cctgaagacg aacgttatac ccatttgatt ggcaaggaat taatcctgcc
    18841 gctgaccggc cgcaccatcc ccgtgattgc cgatgaatac gtcgaaaaag acttcggtac
    18901 cggctgcgtg aaaatcacgc ctgcgcacga cttcaacgac tacgaagtcg gcaaacgcca
    18961 cgacacgcgc ttggttaatg tgttcgactt ggaagccaaa gtgctggcaa acgccgaagt
    19021 gttcaacttc aaaggcgaag cgcaaccagg ctttgccctg cctgaaaaat acgcaaactt
    19081 agaccgcttt gtcgcgcgca aacaaatggt tgccgatttg caggaacaag gcttcttggt
    19141 cgaaatcaaa gcgcatacgc tgatgacgcc gaaaggcgac cgcacaggtt cggtcattga
    19201 gccgatgctg accagccaat ggtttgtcgc catgtccgcc acaccgaacg gcggcgagcc
    19261 tgacagcgaa ttcaaaggct tgagcctcgc cgacaaagcc aaaaaagccg tcgacagcgg
    19321 cacggtacgc tttatccccg aaaactgggt caacacttac aaccaatgga tgaacaacat
    19381 ccaagactgg tgtatctcgc gccaactgtg gtggggacat caaatccctg catggtacga
    19441 caacgaaggc aatgtgtacg ttgcccgcaa tcaggaagaa gccgaaaaac aagccggcaa
    19501 aaccggtttg acccgcgaag aagacgtatt ggacacatgg ttctcctccg cgctcgtgcc
    19561 gttctccacg ctcggctggc cgtctgaaac cgacgaactc aaagccttcc tgccgtccaa
    19621 cgtcttggtt accggctacg aaatcatctt cttctgggtg gcgcgcatga ttatgatgac
    19681 cacccacttc accggcaaag taccgtttaa agacgtgtac atccacggca tcgtgcgcga
    19741 ccacgaaggc aaaaaaatgt ccaaatcaga aggcaacgtc atcgaccctg tcgatttgat
    19801 tgacggcatc gacttggaca agctgctggt aaaacgtacc accggcctgc gcaaacccga
    19861 aaccgcgccg aaagtggaag aagccaccaa aaaactcttt cccgaaggca tccccggtat
    19921 gggcgcggac gcattgcgct tcactatggc gagctacgcc agcctcggcc gcagcgtcaa
    19981 cttcgacttc aaacgcgccg aaggctaccg caacttctgt aacaaaatct ggaacgccac
    20041 caacttcgtc ttgatgaaca ccgaaaacca agactgcggc tacggcgcaa ccgctgccga
    20101 accgcgcggc tattccttcc ccgatatgtg gattgtaggc cgtctgaatc agaccatcga
    20161 gcaggttacg caagcctacg aaacctaccg ctttgatttg gcggcagaaa ccctgtacag
    20221 cttcgtatgg aacgactatt gcgactggta tttggaactt gccaaagtgc agcttcaaac
    20281 cggctgcgcc agccgccaac gcgccacacg ccatacctta ttgcgcgtac tcgaagtcgc
    20341 cctgcgcctg ctgcacccga ttatcccgtt cattaccgaa gaactgtggc aaaccgtcgc
    20401 ccctatgtgc gacgcgaaaa ccgccgacag catcatgctc gcccgcttcc ccgaagccga
    20461 cggcggcgat attgttcaga cggcattcga gcaaatgact gtgttgcagg atttgattgg
    20521 cgcagtccgc aacctgcgcg gcgaaatggg catccagccc aacgtgaaag ccccgctgtt
    20581 tgtcgaaagc gcagacgact tggcggacta cctcaaatac ctgccgatga tgacccgcct
    20641 gaccgaagca cggcaagtcg ccgccctgcc cgaaagcgaa gacgcgccca tcgccgtctg
    20701 caacggtgcg cgcctgatgc tgaaagtcga aatcgacaaa gccgccgaaa ccgcccgctt
    20761 gagcaaagaa gccgagaagc tgcaaaaagc cttggacaaa ctcaacacca aactctccaa
    20821 acccggctac accgaaaaag ccccggcgca tctggtggaa aaagacaaag ccgatttggc
    20881 ggagttggaa gacaaaatgg caaaagttca aaatcagttg gcgaagttga aagactgatt
    20941 tgattgaata aacaaggccg tctgaaaaat gtttttcaga cggcctctt
//
LOCUS       DAWFBO010000021        20689 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFBO010000021 DAWFBO010000000
VERSION     DAWFBO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.021"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(63..239)
                     /locus_tag="WGO87_000532"
     CDS             complement(63..239)
                     /locus_tag="WGO87_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391930.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1600)
                     /locus_tag="WGO87_000533"
     CDS             complement(242..1600)
                     /locus_tag="WGO87_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0391931.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLAALQTLAKVWRENVNPF
                     VFGITGSVGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1724..3371)
                     /locus_tag="WGO87_000534"
                     /pseudo
     CDS             complement(1724..3371)
                     /locus_tag="WGO87_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(3394..4872)
                     /locus_tag="WGO87_000535"
     CDS             complement(3394..4872)
                     /locus_tag="WGO87_000535"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0391932.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDGYGAELIGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCHTRLLGRFNAQNLAACVALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRLLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4897..6642)
                     /locus_tag="WGO87_000536"
     CDS             complement(4897..6642)
                     /locus_tag="WGO87_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0391933.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="WGO87_000537"
     CDS             complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="WGO87_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0391934.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="WGO87_000538"
     CDS             complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="WGO87_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0391935.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="WGO87_000539"
     CDS             complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="WGO87_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0391936.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8680..9897
                     /locus_tag="WGO87_000540"
     CDS             8680..9897
                     /locus_tag="WGO87_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0391937.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEEIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYL
                     PQSHFLLNLGITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVD
                     WAGFRFGDICHKL"
     gene            10029..10105
                     /locus_tag="WGO87_000541"
     tRNA            10029..10105
                     /locus_tag="WGO87_000541"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10063..10065,aa:Met,seq:cat)
     gene            10113..10188
                     /locus_tag="WGO87_000542"
     tRNA            10113..10188
                     /locus_tag="WGO87_000542"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10146..10148,aa:Ala,seq:ggc)
     gene            complement(10479..11300)
                     /locus_tag="WGO87_000543"
     CDS             complement(10479..11300)
                     /locus_tag="WGO87_000543"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0391938.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(11333..11977)
                     /locus_tag="WGO87_000544"
     CDS             complement(11333..11977)
                     /locus_tag="WGO87_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0391939.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11980..12825)
                     /gene="ftsN"
                     /locus_tag="WGO87_000545"
     CDS             complement(11980..12825)
                     /gene="ftsN"
                     /locus_tag="WGO87_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0391940.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKADEVEEK
                     ADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEKKASKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12931..14427)
                     /locus_tag="WGO87_000546"
     CDS             complement(12931..14427)
                     /locus_tag="WGO87_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0391941.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLVQTDCQVPQMPSENTKLLDLVDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAVELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14441..14761)
                     /locus_tag="WGO87_000547"
     CDS             complement(14441..14761)
                     /locus_tag="WGO87_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0391942.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15244..16770
                     /gene="putP"
                     /locus_tag="WGO87_000548"
     CDS             15244..16770
                     /gene="putP"
                     /locus_tag="WGO87_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0391943.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            17021..20626
                     /gene="putA"
                     /locus_tag="WGO87_000549"
     CDS             17021..20626
                     /gene="putA"
                     /locus_tag="WGO87_000549"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0391944.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4839 a         5991 c         5055 g         4804 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaattgggt aactccccgg atttttaccg
      121 ccgcccaagt ctaaacataa atcttcataa agatacgcct gcgtccgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcagcaat aaggctttaa aaaaacgtcg gcttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gggcatacgc gccgacttcg gcgtgcatag
      481 cggcggcttc gtcctcgccc agttcgccca tatcgcccat tacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca atcgcggctt tcatgctgtc cgggttcgca ttataagtat
      601 cgtcaatcag ggttgcgccc ttgattccgg atttgacgtt cagacggcct ttgatattgc
      661 tgaagccttt caaaccttcc gccacatcgt tcaaactcaa acctgcagcc aaagacagcg
      721 cggcggcagc ggcggcgttg tggacattgt ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtc gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag tgccatgccg tctgaacata
      961 agccttgata aatctcgctt ttcgctttgg caatatcgcc cactccgtcg aaaccgcagc
     1021 cgacatgggc gcgcatggcg ttgttgacca atgcggcatc gggtttggcg atttgtgtca
     1081 aaaccgccag ttcgccaaaa tggttcatac ccatttcaat cacggcatag cggtgttttt
     1141 cgtttaattt caacaaagtc aacggcaatc cgatgtggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc attatcgccg aaacggcggc gcaataccgc agccagcatc tccttcaccg
     1261 tcgtcttgcc gaccgaaccg gtaatgccga acacaaacgg attcacattt tcacgccaca
     1321 cctttgccaa cgtttgcaat gcggcaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 caacgcaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcatcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcgga
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgggtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttaggtcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt ttttatcgcc tgttttactg tggtaaaaca
     1681 cagattattt tcccattctc attcggcatt ttttctgtac gtatcatttt ttagacgtat
     1741 ttttagtcga tttgcctttt cccgcatacc acggcgcggg gtcgtcgggc agtccgtcga
     1801 taaaggcaag gttattgcct tcgccctgca catcgggaac attcccccaa aaatcatagc
     1861 cgtcatcggg caactcgtcg gtttcgatac ccgtccaact gccgaatccg cgtaaaaaat
     1921 taaacgcgct gcgcctcact ttaatcattt cgcgccgcgt gtcatcggac gaaattttaa
     1981 ccagcggcac gccgtagctt tgacgcttcc acgcaccatg tgtcaacgtt cgctcgccgc
     2041 ctttaccgac atgcatcaaa ccgtggtcgg aaaagtaaac caaagaccag ctttctttat
     2101 tttcattcaa tatcttaact gtatcttcta aaaatttatc ggtttgcgcg atggtggaaa
     2161 catagcagga tattttttca gtttgatact gaaaccgccg cgcatcctta tccaaacgtg
     2221 tgcaaaaatc actgtgcgaa cccatcaggt gcatcacaat cagccgaggc ttcgtgcctg
     2281 catttcctat caaaacccgt ttgaacgccg gcaacaaaag gcggtcgctc aaccccgcgc
     2341 ttttgccata atcacccctt tgggtaaacc acggataatc gctgcgtagg gcataggtgg
     2401 aaatttcgtt ggcaaaatgc cccaacattc cttgattaga cagccacgcc gtccgaaaac
     2461 ccgcctgctt cgccaagctg acgatgttat tgttcggttc tcccggcagc cccaaagtct
     2521 gcggcagcga aagattcgtc gcgtgggcgg tcgattggta accgtttatc aacagccctt
     2581 tggtctgact caaaaaaggc gtatcgggca atgggaaacc gtaaacattc atataatccg
     2641 aacgcgcgct ctcaccgata accacaacat aatttttata cttgggcgca acatgacgga
     2701 tatgccatgt tgacgctttt tttgctgctt ccaaaatgtg ggcgcgcttg gcggcatatt
     2761 gtgccggcgc ggaagccaaa tcgtaataca ggtcgaaaat attcaacaac aggccggcat
     2821 caggttctcg caaatcttta tcgctggcga ttttatccat caccgcgcag gacaaaatca
     2881 aagtcaataa tatagtcagc catattttgc tgcggcgttt atagtttttt acgtcagcaa
     2941 ataccccccc ccgatacaca atatttccaa actgtcaagc caaaaatgaa aaataatgcc
     3001 tgcacaaaat aaagcgaccc gggaagattg ccgacaaatt cacgcgcctc ggcaggattg
     3061 ctttccaata tcgaaccgac tatctgataa gacggcgcac catacaacca gccgaccggc
     3121 aaatataggg cggttgtgcc gacgtaaatc agtaaaacaa ctgaagaaac gcgcgggaaa
     3181 ccgcgtgcca ataataataa aaatacaacc gaagccaaag cacccacggc agaataacgg
     3241 tagccgtatt catattccaa gtgataacct gatgcgatgg ccgcgccaaa acaaaaggct
     3301 aaaagcgcgc aaatgcccaa tgtatttttt aatgtctgat tcatattttt tattcgggcg
     3361 gattgtttca tacgatgccg tccgaatatc ccattattta cgagttaaca aagcctgtcc
     3421 gacgatttca agatcggaaa aacggtgctt cacgccttgt acatcctgat agttttcatg
     3481 ccctttgccg gcaatcagga tgatgtcgtt tgcggcggct tgttcaaccg cataacggat
     3541 ggcggcggca cggtcggctt cgacgcattc gggcgcggga acggcaggca ggatgtcgtt
     3601 gatgatgtcg tgcggatttt ccaaacgcgg attgtcgctg gtgacgacga ctttatccgc
     3661 gccctgtacg gctgccgcgc ccatcagcag gcgtttgccg cgatcgcggt taccgccgca
     3721 accgaatacg caccacaaag ccgcaccctg cggtttgatt tcctgcaagg tggcgagtgc
     3781 tttttccaat gcgtcgggcg tgtgggcata atcgacaacg accaaaggtt tgccgctgtt
     3841 cataatacaa tccatacggc ctgaagcagg acggattttt tccagtacat ccaatacctt
     3901 atccagcgga tagccgttgg cgcaaagcaa agcgacacag gcggcgagat tttgcgcgtt
     3961 gaaccgtccg agcaggcgcg tgtggcattt cccttcgccc cacggggttc ggaatacggc
     4021 ttctatgccg tctgaagagg cggtaaagtc ggtaatgcgg atgtcggcgt gttcgctgaa
     4081 gccgtaactg taaacggcca aatcggggca gtcttttttc agacggccta tgagttctgc
     4141 gccgtatccg tcatccacgt tgatgactgc gtgtttcaag ccgtgccagt aaaacaggcg
     4201 cgacttgatg gcaccgtagg cttccatcgt gccgtggtag tcgaggtggt cgcgggtgag
     4261 gttggtaaag attgcgctgc ggaatgacac gccgttgacg cgcgactggt caagcccgtg
     4321 gctggagact tccatcgcgg cgactgttgc gccttgttga cggaaacggt agagcagggt
     4381 ttggacatcg acgggggcgg gtgtggtatg cgtggtttct tccaatgcac cccaaaagcc
     4441 gttgccgacc gtgccgacaa tggcggtttt ttcgcccaac aaatcggcag cttgcgccag
     4501 ccattgcgtg atggaggttt tgccgttggt tccggttacg ccccagaatt tgagaccgtc
     4561 tgaaacgttg ccgtaaactt gcgccgccaa tatgccggca cggtgtttca aatctttgat
     4621 gccttgattg gggactttcc attcgggatt ccacgcaaat ttgccgtcgt cgtcccaaaa
     4681 aacaaaagcc gcgccgttgg caacggcggc ggggatataa ctgcgtccgt cggcatattc
     4741 gcccggacag gcaacgaaaa tatcgccttg tttgatttgg cggctgtctg aatgcaacaa
     4801 acgccctgcc gcgtttgcac acgacagggt cgggatgccg gtttcagcca aaggggttaa
     4861 cttgctgaac ataaaacaat ctcgttgata ctcggattaa gacggtgttt tgacggctgc
     4921 ggcggtcagg ggcttggtcg gggaaatgcc caagatgttc agactgccgc ccataatttt
     4981 tttgaagggc ggccctgcca ctacgccgcc gtaataaccg tgggcagtcg gttcgtcaat
     5041 ggttaccgcc acaatcacac gggggttttt ggcgggggca aaaccgataa aggtagcgat
     5101 gtgtttgttg tcggcataac gcccgttgac gaacttgcgc gccgtgccgg ttttcgcgcc
     5161 gacatcgaaa ccgtccaccg cacccgccgt accggtgccg cccggctcgg ttacggaaac
     5221 catcagattg cgtacctcgc gcgcggtcga ttctttgaat atgcgtttgc cttgcggcgc
     5281 aaccgcctgt ttttcaaagc tgaccggcag taaaacgccg tcgtgcgtca gtgcggtata
     5341 ggcgcgcgcc aattgcagca ggctcaattg caggccgtaa ccgaaagaca tcgtcgcctg
     5401 ttcaataggc cgccacctgc gccaatttct caacaaacct gcagtttcgc ccggaaagcc
     5461 cgagtgcata cgcacaccga tgcccaactc atgatagaag tcatacattt cttcggcacc
     5521 gaaacgcgca gacagtttgc ttgtgccgac gttggacgat ttctgcatga tgccgcgcac
     5581 atccaaagag gggtaaacat gggtatcgcg cacgggagac ggtccgattt tataaggctg
     5641 cgtattcagc cgttcgttca aatcggtttt gcccgcatcc aatgctttcg caatcacaaa
     5701 cggtttgatt gccgaaccgg gttcgatcat atcggttacg gcacggttgc gccgctgttc
     5761 gctgtctgcc cggccgggcc tgttgggatc gtaggcgggc gtattggcca aggcgaggat
     5821 ttcccccgtg cgggcatcca aaaccaccac cgttccggct tttgcctgat ggtattcgac
     5881 cgccttgttc aactcttcat aggccaaggt ctgaatcctc tgatcgaggg aaaggatgat
     5941 gtctttgccg tttttcgggg ctttattgcg cggggagtcc aagctgtcca caatattgcc
     6001 ctgccggtcc cgcaaaacga cttccgcgcc gtcttcgcca tgcaggctgt cttcaagcga
     6061 aagttccaaa ccttcctgac ctttgccgtc aatatcggta aatccgatga cgtgtgcaaa
     6121 caggttgccc atcgggtaat ggcgttttaa ttctttttca aatacaaagt tttccaaacc
     6181 caaggctttg acctcttcgg caaccttggg atcgagctgc cgcttaatcc agataaacga
     6241 cttgcctttc tgttcgagct tgttcctcaa aacatcaacc ggcacatcga caagctcgga
     6301 caggcgttcc aattgtgcgg cagacggcat ttccttcatc tctttaggca cggcaaacag
     6361 ggactccgtc ggcgcactca acgccaaaac cgcaccgttc cggtccgaaa ccgtaccgcg
     6421 tgtagccggc aatgtttgag tccgcacaat ccggttgtcg ccctgttctt tcaaaaagtt
     6481 atacgttacc gtctgcagat acagtccgcg agcaatcaga ccggcaaaca agaccgctat
     6541 tgccatcagg acgaagctga tccgtccgtt actggtcatc ggctttttga cctgctcttc
     6601 tttgggcagc atccgaggct tatattcgct cttaatcaac atttttactt ctcgttatta
     6661 ttatcctgac gcaggaatcc gattccggca cacaggctgc ttctatcttt gatgcgccac
     6721 cataaaggta ttgcccgaaa ccggcggatg gaggttttgt ttttctgccg ccgccctgat
     6781 cgcttcgtgg ttcgccaaac gcgcctgttg cagcctcatt tgcgcataat cctgctccaa
     6841 ggcgatttcc tgttttttcg ccttgtccaa agctgtgaag ttgagcctgt actggttttg
     6901 ctgcatcaca acggaaaaag cggaaacgca caccgcaagc agcagaagga aattcaattt
     6961 gttcattgcc attcagacgt ttttctctgt gattgttccg gtatcgaacc ggcagtccgc
     7021 tccgccacac gcaaaaccgc acttctcgcc ctcggattgg cggcaatttc cgcctcaccc
     7081 ggctttaatg ccctgcccac gattttcagg ggcggctcgg gcaaatccgc ttccctgacc
     7141 gccgcccagc gcggcagggg cgcgtgttgc gaatattttt tgacaaactg cttcacaatg
     7201 cggtcttcca acgaatggaa agcaatgacc gccaaacgtc cgccctcttt cagacgacac
     7261 atgacctgcg gcaatactgc ccctacttct tcaagctcgc ggttaataaa gatgcggacc
     7321 gcctggaagg tgcgcgtcgc aggatcctgc ccccgctcgc gagtacggac gttttgtgcc
     7381 acgatctgcg ccagcttgcg ggttgtatcg attggacttt ccgcccgttg cgcaacaatg
     7441 gcgcgcgcaa tctggcggct aaaccgctct tcaccataat tcttgattac ctcgtgcaaa
     7501 tcctgttccg acgcaaccgc tatccactct gcggcagaca taccgcgcgt cgtatccata
     7561 cgcatatcca aaggggcatc gaaacggaag ctgaaaccgc ggctgccgtc atcgatttgc
     7621 ggggacgaaa tccccaaatc aaacagcgca ccgtccacct tgccgatacc caaaccgtcc
     7681 aatgccgtct gaaacgaagc aaaaccgcca tgcacgacac cgacccgttt gtccgaacgc
     7741 gccagctctt ctgccacagc aatcgcctga gggtctttgt cgaaaacaat caaccgcccc
     7801 gcatcgccca aacgcgacaa aatcagccgg gaatgccctc ccctgccgaa cgtaccgtcc
     7861 acatagacac cgtcttcgcg cacggcaagc gcatccaccg cctcattcag caagaccgtg
     7921 atatgccggt aactttctgc tccactcaca attgcaaatc cgtctgaccc aactggaagg
     7981 caagttcgtc aggatcgtca tccaaagcct gaaccatctc agcctcccac tgctcgcgac
     8041 cccaaagctc caaacggttg gcacgaccga ccaaaacgac ttcacggtcg aaatccaccc
     8101 ttttcctcag tccggcagga agcagcaccc ggccggcgct gtcccattcc aaaatttccg
     8161 cgttatgcag caaaagattt tgaaaccgcc gcaaaacagg gttatccgcc acttttaagt
     8221 ttaaaagttg cgccgcaacc ttttcccact ccgcaacagg gtacatcaac agcttgtgtt
     8281 tcgactcgag cgttaccact acggcaggcg tatagaggcg cgacagaatg tcacggaatt
     8341 tggcaggaac agccaaccgc cccttactgt cgatgcttaa ttcgtgtgcg ccgccgaaca
     8401 tgacatgtcc caagccgaaa tcaaaatcac aagggtaaaa gagacacttt gccccacaat
     8461 tccccaccaa tcgacactat aagaaatttt aaacactcgg tcaaatcagg gcatgaaaac
     8521 tcattaacat atctgaaatt tttattcctt ttaaaaacaa taagataaaa aatgacgaca
     8581 acggcacggc gggtgcggta cagaataatc gaaccaataa acaactatat atgattaatt
     8641 taataatata aacacaatat atagtattaa gataaagcca tgacagcacc cgtaccaacg
     8701 tgtaatatgt cgggaaatcc aataaattta cacaagctaa cacttatcat gcccctcccc
     8761 ctcccctctc ccgaagcacg gcaattctcg ctcaaactgc aaaccctcat tgccgaagaa
     8821 atcggcaaac acggcaactg gattccattt tcacgtttta tggaattggt tttatacgct
     8881 ccgcaatacg gctactacac cggcggcagc cataaaatcg gcaataccgg ggattttatt
     8941 accgcaccga ccctcacctc tctgtttgca cagacactgg cacgccaact tcaagaactt
     9001 ctatcccaaa cggcgggcaa tatctatgaa ttcggcgcgg gaaccggaca gcttgccgcc
     9061 gatttgttgg gcagcatttc ggacggcatc agccgttact atattattga aatatcgccg
     9121 gagctggcag cacgtcagaa aaacctgatt caagcacgcg caccggaagc atctcaaaaa
     9181 gttgtccact tgaccgcact tcccgaagcg tttgacggca tcatcatcgg caacgaagta
     9241 ctcgatgcca tgcctgtcga aatcgtccgt aaaaatgaag gcggctcatt cgagcatgtc
     9301 ggcgtttgcc tagataatga ccgttttacc tattcggcac gaccgctgca cgacttacag
     9361 ctatctgcct tggcttccct ctattttcct caaacagatt atccctacac cagcgaacta
     9421 catccgcaac aatatgcctt tatccgcacc cttgcctcaa ggcttgaacg cggaggtatg
     9481 atattcatcg actacggttt tgatgcagcg cagtattacc accctcaacg caatcaaggt
     9541 actctgatcg gacactaccg acatcacgtt atccacaatc cttttgactt catcgggttg
     9601 gccgacctga ccgcgcatgt caactttacc gacattgccc aagccggtac ggatgcagga
     9661 ctggatttga caggctatct gccccagtcc cattttctgc tgaatctggg cataaccgaa
     9721 ctgttggcac aagtgggaaa aacagactcg cccgcctaca tccgtgaagc tgctgccgtt
     9781 cagaaactga ttgaccagca tgaaatggga gaattgttta aagtcatcgc attcggtaaa
     9841 aatatcggcg tcgactgggc aggattccgc ttcggcgaca tctgccacaa actctaaccc
     9901 tcatgccgcc tgaatccgct tcagacgaca taaacttttt aacatttaaa aacagtcaac
     9961 taattcaaaa ttaaaaaata cggcttgtca aaaaaacaga aaaacatata atagtgtctt
    10021 cacgaaacgg cgaattagct cagtcggtta gagcagagga atcataatcc ttgtgtccgg
    10081 ggttcgaatc cctgattcgc caccaaattt tcgggggtat agctcagttg gtagagcgct
    10141 tgcatggcat gcaagaggtc agcggttcga tcccgcttac ctccaccaga taaaaaagca
    10201 cagaccgtaa aaaggtatgt gcttttttat tacctgattg ccgggaaata aagaacaaac
    10261 cactgccttc aaaacaggca atcgacttta aacctatcgc cccgcctgtc ctgattttat
    10321 agtgaattaa atttaaaccg gtacagcgtt ggctcgcctt gccgtactat ctgtactgtc
    10381 tgcggcttcg tcgccttgtc ctgatttttg ttaattcact atatcagccc gccagacaaa
    10441 cccgacccga ataatgtctt caggtcgggt ttatggtttc attcccaact tatccagccc
    10501 gacaaccaca atattatgat gacgatgccg aaaacaatgc ggtaataggc aaacgggata
    10561 tagtttttct tggaaacaaa cttcagcagt gctttgaccg ctactaaacc ggaaacaaaa
    10621 gcggcaataa agcctatcag aatcaaaccg acatcatgca gggtgaaaaa ccggtaatgt
    10681 ttcaggacat cataagccgt tgcggcaacc atcatcggaa cggccaagaa aaatgaaaac
    10741 tccgttgccg ttttccgctc gattccccaa agcatcccgc ccataatcgt actacccgaa
    10801 cgggacgtac ccggaaccag tgcaaacact tgggcaacgc cgatcatcaa cgcatcaatc
    10861 ggacgcaatg catcaacatc ggcaatttta ggctctgccc ggctttggcg tttctccacc
    10921 cacaaaataa aaaaaccgcc caaaaccagc atgaccgcca cactcaaagg attgaacaga
    10981 tgttctttaa tctgtttgcc gaacaacagc cccatcacgg cggcaggtat aaaagcaatg
    11041 gcaagattga ggacgaagcg gttggctttc cggtcttttc ccacgccatg caacacattg
    11101 ctgaaacgct gccggtattc aaacactacc gccaaaactg caccgagctg gatggcaatt
    11161 tcaaaaacct tgtgattgct gtgaaaatca atcagattgc cgaacacaat caaatgtccg
    11221 gtgctggaaa tcggtaaaaa ttcggtaaaa ccttctacca agcccatcat cagggctttc
    11281 aggacaatca gaaaatccat tgcttgcgct tctttcggat acgggagttc ggctatttct
    11341 gtacagcagg ggtctgacgc ttgcgttctt ccctgacttt ggcaaccaat tctttaatcg
    11401 tatgaacgcc gccgtcaaag ccattattga agataacgcg gtattttccg ccgacaataa
    11461 cggtcggcgt gctgtcaata ccgtattgtt ccgtcagttt ctgcattttt aatgcggcgg
    11521 cggcagcttc gggggaatca taggcgcgca tcagtttttt gccgtcaaag cctttttgag
    11581 acaaagccca ttttccggca acagccctgt tttccaagcg gattttttgt tcgtaaactg
    11641 ctttaaacac agcagggttt gcctgatatt tcaaacccga caaattgacg gcagccgcca
    11701 tcctggccag accgagcatt tcaggctgcc agaccacgtg ctccgtcctc aaataggcat
    11761 cagacggcaa tgccttgccc aatttcaata acaaaggatc gaaatgatgg caatgtacgc
    11821 agaaatagcc gaaaaattcc aaaacctcaa ttttgcccga ctgttcttga ggaatgggtt
    11881 tatccaacac aagatagtct tccctttccg tcagggcata tgcctgcgcg gacaacactg
    11941 ccgacagcag cagcggcaac agatgtttga gcttcataat tatttgcttt cgatagaacg
    12001 gatcaggctg gcgacttcat gttttttcaa ctcgtcctgc atttttttca ccgcatcggc
    12061 agacatattg ccgctttgca cccggtaaag cgttttatgt cccgcctgat aaccgaccac
    12121 cttggaagat atgcccaaga ttgccagttt ggcacgctgt ccttccgcgc tccggcggtc
    12181 ggcatacgcg cccatttgca gataatgcgt tgcttccgcc ttgtcgggcg ttttcatttt
    12241 ctgcacttct ttggcagcgg cactgcgcgc tttttcgatg ctgccgctgt tgaggatttg
    12301 ttccggggtc ggtttgggtg caactttttc cttctccgcc tttttctctt cttttgaagc
    12361 ttttttctct gtttctttag atggttttac cgcttgtttt ttaaccgttt cggcatcttt
    12421 cttctgcgct ttttccccga cggtttgttc acgctcttcc gtcagtgctt tcttgcgcac
    12481 tgcctgtccg tccgactttt cccgctccgg ctcgtccgcc ttttcctcaa cctcgtcggc
    12541 tttatcggca gctttttccg catccgactg ctctgcctct ttcgcagcat ccggttcgga
    12601 caaggcgttt tgatcggccg gttcaggttg gatgtcttcc ttaggctggt ttttcggttt
    12661 caggatttcc gtttctgcag gctgcttcga cggaaccggg attttgaacg cattttgacc
    12721 gctctggttc agataaaaca aaataccggc aataatgacc gtcgccagta tcaaaccgaa
    12781 gaaaaaaccg gacagacctt ttccggattg ggaaaatttg ttcataaaca taccttaatg
    12841 tgtttcagac ggcattaacg ccgtttgctt acgggcggat attctaacaa tatcgccata
    12901 tttgggcaaa acctgcttcc attcccgttc ctataaagcg cggcggaaac ctatggcttt
    12961 catgacgtgg cttctgccga cttcttcgtc gcccgccaaa tccgccaatg tacgcgccac
    13021 gcgcatgatg cggtggaaac tgcgggcgga gagggagagt ttttccagca ggctgcccaa
    13081 tgcttcctgc gcttcttttt ggatgcgggc ttgggaatcg agttcgctga cgctcaaagc
    13141 ggcgttgact ttgccctgcc gtgcgtattg tttgtcgcgg gcggcgataa cgcgttccaa
    13201 aacggacgcg ctgctttcgc ccgcttcctg ctgcatcagt tcgacggcgg acaggctggg
    13261 gacttcgatg gtcaaatcga tgcggtcgag cagcgggccg gaaatcttgc tgcggtaacg
    13321 cgcgacgctt tcgggcgtgc agcggcaggg tttgacggga tgcccgagat aaccgcacgg
    13381 acacgggttc atggcggcga caagttggaa tttggcagga tagacggctt ggcgcgccgc
    13441 gcgggaaatg tggatttcgc cgttttccaa cggttcgcgc aaaacttcca aaactttgcg
    13501 gtcgaactcg ggcaattcgt ccaaaaacaa aacaccgtga tgcgccaatg aaatctcgcc
    13561 cggacgcgga tccgaaccgc cgccgaccat agccgccgcg ctggcgctgt gatgcggact
    13621 gcggaaagga cggttgctgt cgagttgttg ctggtggttg ggcaggagcg aacgcaatgc
    13681 ccaaacttca accaattcgt cttcggtcag cggcggcagg atgccgggca gccgttggga
    13741 gagcatagac ttgcccgttc ccggcggacc catcatcaag aggctgtgtc cgcctgcggc
    13801 agcgatttcc aaagcaaggc gggcagtgtg ctgacccttg acatcgacga ggtcgagcaa
    13861 tttggtgttt tcagacggca tctgaggaac ttgacagtcg gtttgcacca aaggttcgat
    13921 gccgttcaaa tgggcggcga cttcgcccaa agagcgcgcg ccgtaaacgg taatgccgcg
    13981 catcacggcg gcttgtcctg cgttttcttc aggcaaaaca aatgcacgtt ttgcctgcat
    14041 accctgccac gccatcgcca acgcgccgcg cacggggcgc aacagccccg acagtgccaa
    14101 ttcccccgca aactcgtatt ccgccagttt ttcgggcgca acctgccccg atgcggcaag
    14161 gatgccgatt gcaatcggca aatcgaaacg ccccgactct ttgggcaggt cggcgggggc
    14221 gaggttgacg gtaatttttt tggcggggaa ttcaaaaccg ctttgaataa tggcggcacg
    14281 gacgcggtcg cgactttcct ttacttccgt atcgggcagc ccgacgatgt tgaaatgcgg
    14341 caggccgttg gcaaggtggg cttccacttc gaccaacggc gcattcatac cgctcaaggc
    14401 gcggctgtaa accaaggcaa gcgacatatt tcagacggcc ttattcgccg gcttcggttt
    14461 gctgcctgat ttcggcgacg gcttcttcgg cagcggcttc agccgcttcc aatgctgccc
    14521 gttcgggatt ttgcgcggct tcgagttttg ccaaacgcgc ttccaaagcc gccagtttgg
    14581 tacgagtttt gattaaaacc tgctgctgga tgtcgaattc ttcgcgcgta accagatcca
    14641 tacggttgaa cgcgccgccc agcatcgcct taatattttt ttccacatct ttggcagggc
    14701 tgttggcgat ggtttcgctg attttcgaac cgacttcttc aaaaagctgc ttgccgaaca
    14761 taatctgtat ccttcctgaa catatcaaat tcaatcggct attgtataag gaaaaatgcc
    14821 gtctgaaaac gggcggcgga taatcggcaa aacataccgc gcctcctttg cggttgctaa
    14881 attacacctc aaaacacctg ccgcctgaaa acggatttca tattccgcgc aacgccagat
    14941 tacaagacac tacaaaacaa tatactgttt taaatgattt ttccgacgcg catcgtttca
    15001 aactcggctt tttaagccat taagtgcttt gcaaacaaca gaaattgggt tatcctgaaa
    15061 cggattattt acaatttcat atagttttat tacatatctt attgtgattg aagataattt
    15121 atccgaatcc ccctttcggg tatccggatt ttccgttgta cttttattag aaaaacattt
    15181 caggcgcaag ttgcttgcaa tttcaaagcc tgtaattctg aagttttaga cgaggaatag
    15241 cacatgaatc ccatgtacat tacttttgca atctatctgg ttgcagtcct tctcatcggg
    15301 cttgccgcct atttttccac gcgcaatttc gacgattata ttttgggcgg gcgcagccta
    15361 ggcccgtttg ttaccgcgat gtcggcaggc gcgtccgata tgtccggctg gctgctgatg
    15421 ggtctgccgg gcgcgattta cctgagcggt ttgaatgagg cttggattgc catcggcctc
    15481 ttggtcggcg cgtatttcaa ctggctcttg gtggcgggcc gcctgcgcgt gcataccgaa
    15541 tacgccaaca atgcgctgac gctgccggat tatttcttcc accgcttcgg cgcgggcgga
    15601 cacttgatga aagtggtttc cgcactgatt atcctgttct tcttcacgat ttattgcgcc
    15661 tcgggcattg tggcgggcgc aaccctgttc caaagcctgt ttgaaggtat gacttacaat
    15721 caggcaatgt ggctgggcgc gggcgcgacc atcgcctata ccttcttggg cggtttcttg
    15781 gcggtcagct ggacggatac gctgcaggct tctttgatga ttttcgccct gattttaacg
    15841 ccggtgatgg tctatctggg cttgggcggc gcggaacaga tgtctgccgc gattcaaagc
    15901 gtcgccgcag gcacgggcaa agaatacggc agcctgtttg ccggtacgac cgtcatcggc
    15961 atcatttcca ccgccgcatg gggcttgggc tatttcggac agccgcacat tttggcgcgc
    16021 tttatggcgg ccgaaagcgc gaaatcgctg gtatccgcac gccgcatcgg catgacttgg
    16081 atggcgttgt gccttgcggg cgcggtggcg gtcggttatt tcggcattgc gtatttcggt
    16141 gcaaatcccg acaaagtttc ttctatgagc ggcaaccacg aacgcatctt catcgcgctt
    16201 tccaccctgc tgttcaaccc ttggatcgcc ggcatcattt tgagcgcgat tttggcggcg
    16261 gtgatgtcca ccctgtcctg ccagcttttg gtttgctcca gcgcgattac cgaagacttc
    16321 tacaaaggct ttttgcgtaa aaacgcgcaa cagtcggaac tggtatgggt cggccgcctg
    16381 atggtcttgg cgattgccgt gatttccatc ctgattgctt ccgatccgaa cagcaaagta
    16441 ttgggactgg tgtcttacgc gtgggcaggt ttcggtgcgg cattcggccc gattgtgatt
    16501 ctgtccgtat tgtggaaacg catcactgcc tacggcgcgt tgtcgggtat ggttgcaggt
    16561 gccttaaccg tggtcgtatg ggcggaatgg gtgaaaaaac cggctcaagc ggcaggcgaa
    16621 agcggcttgt tgacgatgta cgaaatcgtc ccgggcttta ttgtttgctt gatcgttgcc
    16681 gtgttggttt ccctgttcaa caaagaacct tcgcgcgaaa cccaagaacg ctttgaaaaa
    16741 gccgacgcag attaccgcgc cgcccgataa acccctttca gacggcacgg catcccggtt
    16801 cgtgccgtct gaacccgttt ccgggtcaaa cacaaataaa ccttgcctgc atacaggcct
    16861 tatttgtgtt ttacccgttt cattttcaaa gaaacggaag cacctaccct gcccgcattt
    16921 gaagccttgt atttcatctg cggatttatt acgatattac cgtattcaga ccgcaccgat
    16981 gccgtctgaa ccccccgaaa aactttggag aatccaaaaa atgtttcatt ttgcatttcc
    17041 ggcacaaact gccctgcgcc aagcgataac cgatgcctac cgccgtaatg aaatcgaagc
    17101 cgtacaggat atgttgcaac gtgcacagat gagcgacgaa gagcgcaacg ccgcctccga
    17161 gcttgcccgc cgtttggtta cccaagtccg cgccggccgc accaaagccg gcggcgtgga
    17221 tgcgctgatg cacgagtttt cactctccag cgaagaaggc atcgcgctga tgtgtctggc
    17281 agaagccctg ctgcgtatcc ccgacaacgc cacgcgcgac cgcctgattg ccgacaagat
    17341 ttcagacggc aactggaaaa gccatttgaa caacagccct tccctcttcg tcaacgccgc
    17401 cgcctggggc ctgctgatta ccggcaaact gaccgccaca aacgacagac aaatgagttc
    17461 cgcactcagc cgcctgatca gcaaaggcgg cgcaccgctc atccgccaag gcgtaaatta
    17521 cgccatgcgc ctgttgggca aacagttcgt aaccggacag accattgaag aagccctgca
    17581 aaacggcaaa gaacgcgaaa aaatgggcta ccgcttctcc ttcgatatgt tgggcgaagc
    17641 cgcctacacc caagccgatg ccgaccgcta ctaccgcgac tatgtcgaag ccatccacgc
    17701 catcggcaaa gatgcggcag gacaaggcgt ttacgaaggc aacggcattt ccgtcaaact
    17761 ctccgccata catccgcgct actcgcgcgc ccaacacggc cgtgtgatgg gcgaactgtt
    17821 gccgcgcctg aaagagctgt tccttttggg taaaaaatac gatatcggta tcaacatcga
    17881 tgccgaagaa gccaaccgtc tggagctgtc tttggatttg atggaggcac tcgtttccga
    17941 tcccgatttg gcaggctaca aaggcatcgg tttcgttgtc caagcctacc aaaaacgttg
    18001 tccgttcgtt atcgactacc tgatcgacct tgcccgccgc aacaaccaaa aactaatgat
    18061 ccgcctcgtc aaaggcgcgt attgggacag cgaaatcaaa tgggcgcaag tggacggctt
    18121 gaacggctat ccgacctaca cccgcaaagt ccacaccgac atctcctacc tcgcctgcgc
    18181 gcgcaaactg ctttccgcgc aagacgcggt atttccgcaa ttcgccaccc acaacgccta
    18241 caccttgggc gcaatctacc aaatgggcaa aggcaaagac tttgaacacc aatgtctgca
    18301 cggcatgggc gaaaccctgt acgaccaagt cgtcggcccg caaaacttag gccgccgcgt
    18361 gcgcgtgtac gccccggtcg gcacacacga aaccctgctc gcctacttgg tgcgccgcct
    18421 gttggaaaac ggcgcgaact cgtctttcgt caaccaaatc gtcgatgaaa acatcagcat
    18481 cgacacgctc atccgcagcc cgttcgacac catcgccgaa caaggcatcc acctgcacaa
    18541 cgccctgccg ctgccgcgcg atttgtacgg caaatgccgt ctgaactcgc aaggcgtgga
    18601 cttgagcaac gaaaacgtat tgcagcagct tcaagaacag atgaacaaag ccgccgcgca
    18661 agacttccac gccgcatcca tcgtcaacgg caaagcccgc gatgtcggcg aagcgcaacc
    18721 gattaaaaac cctgccgacc acgacgacat cgtcggcaca gtcagctttg ccgatgccgc
    18781 gcttgcccaa gaagcggttg gcgcagccgt tgccgcgttc cccgaatgga gtgcgacacc
    18841 tgccgccgaa cgcgccgcct gcctgcgccg tttcgccgac ctgctcgaac agcacacccc
    18901 cgcgctgatg atgcttgccg tgcgcgaagc aggcaaaacg ctgaacaacg ccattgccga
    18961 agtgcgcgaa gccgtcgatt tctgccgcta ctacgcaaac gaagccgaac ataccctgcc
    19021 tcaagacgca aaagccgtcg gcgcgattgt cgccatcagc ccgtggaact tcccgctcgc
    19081 catctttacc ggcgaagtcg tttccgcatt ggcggcaggc aacaccgtca tcgccaaacc
    19141 cgccgaacaa accagcctga ttgccggtta tgccgtttcc ctcatgcacg aagccggcat
    19201 cccgacttcc gccctgcaac tcgtcctcgg cgcaggcgac gtgggtgcgg cattgaccaa
    19261 cgatgcccgc atcggcggcg tgattttcac cggctcgacc gaagtggcgc gcctgatcaa
    19321 caaagccctt gccaaacgcg gcgacaatcc cgtcctgatt gccgaaaccg gcggacaaaa
    19381 cgccatgatt gtcgattcca ccgcacttgc cgaacaagtc tgcgccgacg tattgaactc
    19441 cgccttcgac agcgcgggac aacgctgctc cgccctgcgt atcttgtgcg tccaagaaga
    19501 cgttgccgac cgtatgctcg acatgatcaa aggcgcgatg gacgaactcg tcgtcggcaa
    19561 accgattcag ctcactaccg atgtcggccc cgtcatcgat gccgaagcac agcaaaacct
    19621 gttgaaccac atcaacaaaa tgaaaggcgt tgccaagtct taccacgaag tcaaaaccgc
    19681 tgccgatgtc gattccgaaa aatccacgtt cgtccgcccc atcctgtttg aattgaacaa
    19741 cctcaacgaa ctgcaacgcg aagtcttcgg tcccgtcctg cacgtcgtcc gctaccgcgc
    19801 cgacaaactc gacagcgtca tcgaccaaat caacagcaaa ggctacgccc tgacccacgg
    19861 cgtacacagc cgcatcgaag gcacagtacg ccacatccgc agccgcatcg aagccggcaa
    19921 cgtttacgtc aaccgcaaca tcgtcggcgc agtcgtcggc gtacagccct tcggcggaca
    19981 cggtctgtcc ggcacaggcc ccaaagcagg cggttcgttc tacctgcaaa aactgacccg
    20041 catccccgaa tgggttgccc cgaccctgag ccaaatcgga caggcagacg aagccgcgct
    20101 caaacgcctc gaagcactgg ttcacaaact gccgttcaac gccgaagaga aaaaagccgc
    20161 agcggccgct ttgggacacg cccgcatccg caccctgcgc cgtgccgaaa ccgtccttac
    20221 cggcccgacc ggcgagcgca acagcatctc atggcacgcg ccaaaacgcg tctgggtaca
    20281 cggcggcagc ctcctgcaag ccttctgcgc cctaaccgaa cttgccgcct ccggcgtaca
    20341 ggcagtggtc gaacccgaca gcccgctggc ttcctacacc gccgacttgg aaggtctgct
    20401 gctggtcaac ggcaaacccg aaaccgccga catcagccac gttgccgccc tgtcgccttt
    20461 ggacagcgcg cgcaaacagg aacttgccgc ccacgacggc gcactcatcc gcatcctccc
    20521 ttcggaaaac ggactcgaca tcctgcaagt gtttgaagaa atctcttgca gcgtcaacac
    20581 cacagccgcc ggcggcaacg ccagcctgat ggcggttgcc gactgatttt accgaaatcc
    20641 ccgagcgcgg cccgtgaacc aatgccgtct gaaaaccttt cagacggca
//
LOCUS       DAWFBO010000022        19797 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFBO010000022 DAWFBO010000000
VERSION     DAWFBO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.022"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(209..284)
                     /locus_tag="WGO87_000550"
     tRNA            complement(209..284)
                     /locus_tag="WGO87_000550"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Asn,seq:gtt)
     gene            complement(377..736)
                     /locus_tag="WGO87_000551"
     CDS             complement(377..736)
                     /locus_tag="WGO87_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0391945.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(759..1403)
                     /locus_tag="WGO87_000552"
     CDS             complement(759..1403)
                     /locus_tag="WGO87_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0391946.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1405..1941)
                     /locus_tag="WGO87_000553"
     CDS             complement(1405..1941)
                     /locus_tag="WGO87_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0391947.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1953..3050)
                     /locus_tag="WGO87_000554"
     CDS             complement(1953..3050)
                     /locus_tag="WGO87_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0391948.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3053..4489)
                     /locus_tag="WGO87_000555"
     CDS             complement(3053..4489)
                     /locus_tag="WGO87_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0391949.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="WGO87_000556"
     CDS             complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="WGO87_000556"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0391950.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5479..6126)
                     /locus_tag="WGO87_000557"
     CDS             complement(5479..6126)
                     /locus_tag="WGO87_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0391951.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="WGO87_000558"
     CDS             complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="WGO87_000558"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0391952.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            complement(7300..7437)
                     /locus_tag="WGO87_000559"
     CDS             complement(7300..7437)
                     /locus_tag="WGO87_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ0391953.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            7501..8577
                     /locus_tag="WGO87_000560"
     CDS             7501..8577
                     /locus_tag="WGO87_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0391954.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWIAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8784..10250)
                     /locus_tag="WGO87_000561"
     CDS             complement(8784..10250)
                     /locus_tag="WGO87_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0391955.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10448..11371)
                     /gene="speB"
                     /locus_tag="WGO87_000562"
     CDS             complement(10448..11371)
                     /gene="speB"
                     /locus_tag="WGO87_000562"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0391956.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11470..13362)
                     /gene="speA"
                     /locus_tag="WGO87_000563"
     CDS             complement(11470..13362)
                     /gene="speA"
                     /locus_tag="WGO87_000563"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0391957.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVCPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13529..13813)
                     /locus_tag="WGO87_000564"
     CDS             complement(13529..13813)
                     /locus_tag="WGO87_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0391958.1"
                     /translation="MKKLLLAAVVSLSAATAFADDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="WGO87_000565"
     CDS             complement(13964..15772)
                     /gene="aspS"
                     /locus_tag="WGO87_000565"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0391959.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGGFPRMPYAEA
                     MFYYGSDKPDMRIGLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15830..16531)
                     /locus_tag="WGO87_000566"
     CDS             complement(15830..16531)
                     /locus_tag="WGO87_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0391960.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16674..17822)
                     /locus_tag="WGO87_000567"
     CDS             complement(16674..17822)
                     /locus_tag="WGO87_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ0391961.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17982..18245)
                     /gene="rpsT"
                     /locus_tag="WGO87_000568"
     CDS             complement(17982..18245)
                     /gene="rpsT"
                     /locus_tag="WGO87_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0391962.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18548..19690
                     /locus_tag="WGO87_000569"
     CDS             18548..19690
                     /locus_tag="WGO87_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0391963.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5097 a         5111 c         4603 g         4986 t
ORIGIN      
        1 gcgagccaat gccgtactgg tttttgttaa tccactataa aatcgagttt atcctagttg
       61 tccaggacag cccctatttt ttcataacca tcacgaaagg aattttgcaa agattttatt
      121 ccatctcaaa acaatcatct caaaaatgcg tttctgaccg ccggtaaaac aaaaccctct
      181 aagaaaatac ttagagggtt ttgaaatttg gctccccgac ctgggctcga accagggacc
      241 tgcggattaa cagtccgtcg ctctaccgac tgagctatcg gggaataagc gcgaataata
      301 aaacagaaat accgaacgtg tcaatataaa ttgacaatcg attgcacttt atctgcaagc
      361 ccgcaatctt tatatgttat atggcttccg caatatgcct tgccgcacgt ttggcatcca
      421 ataggattaa accaagtctg ccggtttctt ttgccaccag caccaacacg gcatctttac
      481 ccgcctgact taaaaggata tagcctgatt ttcctttaat catcacttgt tccaattccc
      541 cgcaggcgag ttcctgcacc gagcgactcc ccaaagcaag caaagtggca gaaatcgccc
      601 ctaccctgtc cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat ccgtcggttg
      661 agataacggc agacgcgata atatccgtag atgtattgtt taaatcttca aggattgaaa
      721 tcaataattg ctgcataacc cctcctctct cccaagtttt acacgcggtt gctgtaacgg
      781 cggtataaaa tccttaccaa agtaacaaat gcctctttgc ccaaatcggg aatgccgccg
      841 ataaccaaaa taaatttggt tgaaccgata tacaatggga aaaatgtcaa ttcgctctga
      901 ccggaaggat cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt cttaatcagc
      961 agccggtatt tcttttccat ctgtgcgact tctgccgcca acaaccccaa ctcttccgcc
     1021 gcctcatgat ggaaattggc gttggcaaga tacagaccgt tccgatcgac caataacgcc
     1081 ttaccgctgc cggacaattg ttccatcagc aacggcaatt gctcgtccga caaattgatg
     1141 ccgtctgaat gaccgttttc atcgccatag aggaattcga gtttttgcaa acggtacaac
     1201 aggttcaaag cggtatcgat gtcggcggtg tccgcccaag taagcagctt ctcactgctg
     1261 accatttcgt ccgcatccgc tttcaacagg ctgtgcaaca aagttttacc ggcactgggg
     1321 gcatcgctgg atacggcata aaatgcaccg gcaggagtca ggcggggata taaatttgct
     1381 tgtagtgaaa gtgttgattc catattaaac ctccagtccc ggatcaatag aaaataacat
     1441 tgcgctaacc aattgtttta cgtcatcttc cttacgggca tcaatttcaa aaaccggaac
     1501 attaagatta tgttttgcaa gatatttgtg atacacgtcg ataccgggct gagagcgtat
     1561 atccatcttg gtaataccga caacgacggg tgccttctcc agcagccctc gaaacgaatg
     1621 taaaaagaat tccaaatctt tcaacggatt ggttcgggca ttatctaaaa gcaagaccaa
     1681 acccatactg ccttggctta agatttccca cataaagttg aaccgttcct gaccgggcgt
     1741 accatataaa tggactttgg tatcctcatc caagctgatg gccccgtagt ccatcgccac
     1801 tgtcgtattc cttttcctat ccaaagtcat atcggatgcg gaagcatcgg tctgaacgag
     1861 tgcttcgtcc gaaatagccg caatggcagt ggttttccct acgccgacag gtcctgtgaa
     1921 aataatttta ttttctctca tctctccgcc tcttagctgc ccaacagttt tttcatcagc
     1981 ctttgcagaa ggccgcgcga ctgggattgg gatggtgttg tgattttttc cgcttttttc
     2041 atcatttcac tatcagaggc agaatcggct ccgatattta ttttatccgc catatcggag
     2101 tatgcctgtt gtgaaaccgt ttttaaatct accgacaaaa acccggttgt ataggttgcc
     2161 gcaagataat tcagaatatc attgaggttt aaaggcatca ctttatacaa cacgttaagg
     2221 ttgacggatg ccttggtcag aaatgccgac aagcgtatcg accctggcac atttgccaac
     2281 cgggtcaggt ttggccaaga tttcaacgta aacggagtat cgggagaaat cggataaatc
     2341 aacctgccct gcgccgtcca aatggaaaac tgccacatac aggacataat gcctacttta
     2401 gccttttcgc gccattgcgg gttatcggga acagtcttgc agctgacctg caaattttcg
     2461 tctttgcaca attcttcgag tttttgcaca ctttctgtca gcaaaacccg ttgtatcgag
     2521 gggaaaacaa taaggaccgg cttatttcca tgcaagatag cgatgtcctg cctgttcttt
     2581 tccgcaaacc gcaacgcccc caataatcct ttattcgggt taaactggcg tatcgtaacc
     2641 gtacgctgca cattcccgtt atttttcgcc gaccggtctg cgggtgcaat aaacgatttc
     2701 ccataaacat tctcgccctg caacaatttg cggagcatag gaaacaatgt ttcaaaccga
     2761 atcggtttgg gcaggtaggg aacttcagaa tcgggaactt tctccgaaca gacggcgacg
     2821 ggtatatcct tataacgctc ggcaagctct ttccaaagtt caaaaccgcc ctcggcatcg
     2881 gtatccgcca aaaccaaatc gggcacggca ctgccgtctg aaggggatac tgtttcataa
     2941 cgggtggtat tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg cgccgtcatc
     3001 cccgccaaca ttacgcgtac tgttttaatt ttcggcagtt gaacttccat ttttattttc
     3061 cgtaccgttt tattttttta atattttgat tcatacgctg cagcagccgg ctcatcagca
     3121 taacgacctc ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa aactgcccca
     3181 acctatccca atcttcagta cgttcataaa tatcgatcaa cgtaatataa agctgggact
     3241 cgtcgggata tttcaatacc gcctgctcca acacatccat tgccgcttca atctgaccat
     3301 acatcagcaa cgactctact tccttaaccg catcgtctgc cggagacgaa ccggtgttga
     3361 tcaacgaaga atcttgaagc accaaatccc gatgttgcga tttgaatttc tgtatatttt
     3421 tcggcagata cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc ggcccttttt
     3481 ccaaatcatc gaaaacttca tggtaaccca agctgtaccc ccaccccagc atccgctctt
     3541 taacctgcct gccgtagttg cccaacgttt ggtaaagttt ccacaaatgt ccggcaaaac
     3601 ggtctatgtc cgcgtgttgg taatcgagtt tcaacgcatc gataatcagg tttgcaggtt
     3661 tttcggaagt ttggatggca cggtcgtatt gtttcgatgc cgtttcatag ctgactttgt
     3721 ctttaaggat tttcgcacct tgatcggcac ggaccaaacc ggcaatcgca ccgatttcct
     3781 cttgactgat tccggacacg tcttttttgc cccgcacaat cgggatgcgc ttgatttctt
     3841 cggcttcata agccttaccg ccggcatccg gtgggggcga tgcggaagct gccgtcgcag
     3901 aaccgccttc cgcacgtttt tcaatctcct gagtacccca tcccaaaccc tcttctgcca
     3961 agacgcggat acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata tattttgcca
     4021 aaagttcgga aggaatcagt ttgccgtatt tttgcagatt gtctgccaaa acatcgacat
     4081 cccccacttc gagattgata tcgagcagct cgcggataag gttttcaggt ttcgcttcac
     4141 catccggaat gccgtccaga taggcagcca aagattcggc agccttgccc tgataaccga
     4201 attgcttata aacctgatac tccgtaagcg gatcgacttc ttgcgcggat acggcggcgg
     4261 atgcggtctc ggcactttcg ttccaagacc agtcgggttg cgtgccgtct gaaaccgatt
     4321 gtccaatctg atcgacccag tccgaatcat tgcttccggg tgtctgcccc tgcgtattgc
     4381 cgacaccttg ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc ataaccaaaa
     4441 gcatcagcaa taccgccagt gccaaaccga taattaatga gttttccaaa ggatactccc
     4501 gataccgtaa cgagcggata gctgaccgcc tgcaacggcc attgtccgct tttattatta
     4561 acgatttact gaaatagact tgtaagtttt aaatcatacc ataatttaac gtttaacaat
     4621 atgccttctg cacaagcctc gccatattac cttttaccca catcagtatc aatacccgat
     4681 ataaaaataa ctttgcccat aagctgcctt attgcctgcc cgccgcagag tagcgcgcga
     4741 taaaaaataa cggatgataa gtcaggtgca ctttgcccga cggcatactg aacgccgaca
     4801 agtaatcgcc aataaatcca ttgagatttt ttcttgtcca atttttttgg ttcgtggctg
     4861 ttacgcctgt atatttaagg tgtttgagta catctaacgg cgtatcaaag tctagtatta
     4921 ccttaaaatc ctcacaccat aaaagctcaa aatctttggc taaccaagtc tgccattggg
     4981 ataaagtcgg gtaatttaag cctatatttg taatttggcg gacttctttt aaattgtctt
     5041 tgccaaaggt tgcaaccgcc aataatccgt ttgctttcaa gcctgttttg caatgggcga
     5101 taaaagcgtc gggttgatga aaccattgca cggcagatgc gcttgcgatt aagtcaaatt
     5161 gtcgttgaaa aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat tgcggcagtt
     5221 tttcagccag ttggggctgc acatcgcaca aatcattaaa taaccaataa ttcgctgaaa
     5281 tctgtttttg cagcaaggca ctcaacattc ctgagccgca acccaattcc aacacgtttt
     5341 ccaatggcat atccggcaaa taatcttgca aatgcgccat tagattaatc gtcatttttt
     5401 gttggattaa ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc tgccgaatgc
     5461 gcgatttatt tatggcagtc agtgattcca tagtgccgac caatgggtga atcttgaaaa
     5521 caggtaatgt ccgccgtcaa tttcccgaac ggtgcaacgc ggtgtccaat atcggtgctg
     5581 attggcaggc ataaaaattt tatcgcccga tccgaccaag gcatttgtcc agcgaatcaa
     5641 atctgcgcgc ctatcttgcc cgatcatcgc aaaaagtgcg ataagttctt gatgaatttc
     5701 gtcaaacgga cgggcgggaa attgttggta atcttcaaaa gatgctttat cgccacacat
     5761 tctgcgttca aattttaaac gggtgttttc cgtgaggttc tccaatgtgc ctttaaaaac
     5821 ggcgcaaggg ataccgaaat tatcatcgca aggcaaacct gtgccattca ctgccgttgc
     5881 ggattttaat cttattcctt gcaatgccct ctctgccgcc caaacgccca ttgaccacgc
     5941 caccaaacgg atgtgccgat aggcggaaaa atcaaaatcc aaatttaaat cttgataatc
     6001 atagcaaatc aataaatcgt gattttccgg caaaatcaaa tgatttacag catcgggcgg
     6061 cgttccccaa cctgcaaaat acaggattaa atgttcgcct tgatgattgt aaaattttgt
     6121 tttcatatca tatccttaca ggcacgccgc aaactgccgc acttcatcca ttgtcatatc
     6181 tgccgtcaaa gacaatcgta ttctggatgt accttttggt actgtcggtg gtctgatggg
     6241 caggcaataa taaccctgcc tttgcaggta ttcagctttg gcaagggtgg cttcattccc
     6301 gcctaaaata taggggacga tacaggtttg gctcggcatt atttgcgtcc gatgcgccac
     6361 ttcccgccgt aaaaatgcgc ttaactgctc aagatggctt ctttcttttg agaattgcgg
     6421 caatcgttca aaaataaaat aagtccaagc cacattaaac ggcggcaatg cggttgaaaa
     6481 aatcaatggg cgcatttgat taatcaaaca ttctttcaat acttggttgc agacggcata
     6541 cgcccccacc gaggctaagg ctttaccgaa agtgccgacc aataaatcaa tctcggcaat
     6601 caaattatcc cgttcggcaa tccccaatcc gttttgccca taaacaccga ttgcgtgggc
     6661 ttcatccaca taaagataag tattgggaaa ctgttttttt aattggacaa gctgtttcaa
     6721 atccgccaca tcgccgtcca tactgaaaac agattcggta acgataaaag tgcggtcaaa
     6781 ttttccgacg tttttttcaa gcagattttt caaatgttca taatcattat gacgataacg
     6841 gaaaaacgca caccggctca aacggatgcc gtcaatcata ctggcgtgaa caaatttatc
     6901 tgccaaaatc aaacttttcg tcgtcgtcaa agcaggcaaa ataccgagat tggcgtgata
     6961 gccgctgttg aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa gctcttccaa
     7021 atcggtataa ataggaaagt tgcccgttaa taaacgcgat gaagaactgg taaaagaggg
     7081 aaaattaccg ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg atgccaaacc
     7141 caaataatca ttagacgaca tattcagcat tttgcagttt tcccgcgtaa tataccgccc
     7201 ttgatgaatc aaatccggaa tcgaacgata ttggttttgc gcgccgagtt gttcaagctg
     7261 ttgtttaaaa accttcatta tgatgtaaat attcctgagt taaagcctga accaacccag
     7321 catcaaacaa tcaagtaatg cccttttaat aaaaaccgtt ttgccgccga cctttgccac
     7381 acccctgcct tcataatcca aggcggaaat ttccgcgaca tttgtttccg ttgccattac
     7441 ccaatccgtc cgcactgtac cgaagcgcat attttcgcac aaattggtac ggtttcaggt
     7501 atgatttgcc gaaaacctat cggacacaca ccatattttg atatgaataa aacaatctgc
     7561 cgcactgctg cactattgat aagcggtttt tcatacgcaa acaccgtcat tcccgatgtc
     7621 tcccccgtcg cacaaggtca acatgtcttc attaacatcc ctcagcaacg cctgttcctc
     7681 tacaccgacg gcaaactgac caaggtttat cccgttgcag taggtcgggc gatgacgcaa
     7741 accaatctag gcgaacataa aatcggtgcg aaagcctata accctgtttg gtacatcccc
     7801 aaatccatac agaaagaacg cggcgacggc gtaaaaacca tagccgccgg cccggacaac
     7861 cctttggggc cggtttttgt ccgcttaggc gaccccaaac tcggtttggg tatacacggg
     7921 accaatgcgc cggccagcgt ccccggggtt cggagtcacg gctgcgtccg catgaaatcg
     7981 cccgacgcgc tcgagtttgc caaaaccatc gccagcggct cgcccgcctc cgtcatctat
     8041 caaatggcgg gtctcaatga agatgcggat cgcaacctgt ggattgccgc cttccgcgac
     8101 ccttacggta agaacaacct tgacatcgcc tctctgaaaa aaagtattgc gcaatgggca
     8161 aaaacacagg gtaaaaccat cgcgcccgag aaagtcgatg ctgtactcaa agaccgcacc
     8221 ggatcggccg tctgcctgac ctgcggcaaa aacggcaaga tgaagatgcc gctcaaatcg
     8281 ctggcgtgga tacagggttc ttcctcatac agccaagctg aagtcattga acaaactgag
     8341 gaaaccaact ctgccgaaat atccgagaca cgcacgcccg aagtgcctga tgtacacacg
     8401 cccgaggcac aaccgcattt aaatacccaa tccgacggca cgccgactgc ctataccgaa
     8461 ccggctgccg attcatcgcc gcaagtagaa acacctgatc aggcagcttc cgagccggtt
     8521 gatgtattat tttcaataga tgtgatacgg cagggaaatt tgcgtttagg taattgaata
     8581 accttctgat tatttaatgc ttatgtttat cagaagttga taggcggttt ggtttttgtt
     8641 tggcttttcc tacaccgccg cctgccgctt tctgcaacat tcaagcgcac aaatatgcca
     8701 tctgaaggct ttagacggca tatttcacga taatagtcgc ccaatcaaat aaacacagat
     8761 tggaaattta ttttattttt cccttaccaa taccccagaa ctttccacca aatgctgccg
     8821 ataacgaaaa aaatcagaaa attgactacg ctcatgataa aacccgcctt ccaccattct
     8881 cccattgtgg tgtagcccga accgaaaatc acaggcgaag taccggtcgc ataatgagtg
     8941 agggtcatca taatgttgga tgcggccgcc atcatcagcg cggtcggcat cgccggggca
     9001 ttcagtgaaa cggcagcagc gaaaaatgcg ccgaacatag cggtaatatg tgcagtagta
     9061 ctggcaaaca tataatgcgc atacatataa gcaagcacga ggattacgcc cgcagccgtg
     9121 ccgctaacgc ccaaaccgcc gacactttcc gccaacactc cggagaacca tttaatcagt
     9181 ccgagtttat ttaaaaatgc ggccatcata atcaatgcgc caaaccaaat aatcgtatcc
     9241 cacgcgcttt tttctttcaa aacatcgtcc caagtcaata caccggaaag caaaagcagg
     9301 cttaatccga taaatgcggt ggcggtggcg ttgatactaa aagcgtgatt gccggtaata
     9361 agggcgggaa catctgccca caacagcaac aagataccga aaatgaccgc cataatgatt
     9421 tcgtctgccg acattttacc catctccctc agacggtctt tggcaaattg aacggcattg
     9481 ggcgtttctt taatttcagg cggatacaaa aaatataaaa tcaaaggcat aacgaaaaag
     9541 gcgataacgc cgggaacagc cattgcccac gcccacgccc cccaagaaag acggaaacta
     9601 ctgcctaaat tttcggcaat caagttgacg attaaagggt tgggggcagt tgcagtaata
     9661 aacatagccg acgaaatggg attggaatga tagttgacca aagccaaata tttacccatc
     9721 ttgccttctg tgccttttgc gggattggag ccgtaactgc cggcaatcga ctgcataatc
     9781 ggatgtataa tgccgccgcc gcgcgcggta ttggaagggg taacgggagc cagcagcagt
     9841 tcggaaagag cgagactgta accgatgccc agcgtttttc ttccaaaaac ggcgataaac
     9901 aaatatccga tacgcatccc cagccctgtt ttgagcaaac cgcgcgaaat cataactgcg
     9961 atggcaatca gccaaatcaa cggattggcg aacgcactca acgcatcgct catcgccgcg
    10021 cccggtttgt cggcggttac gccggttact gcgaccaacc cgacggcaat aatcgacagc
    10081 gcgcccaacg gcatggcctt gccgataatg gcggcaatca caccgacaaa catggccagc
    10141 agcgtccaag cctgaggctt gaccccgtcg ggtacgggca gtgccaaaac cagggcgcac
    10201 aatactgcgg caatggcgag gggtatcggt ttgaaaccca atttcatcat attgacctcc
    10261 gtaaaaaaga ccgtcccgaa aaatcggaaa aataatattt aactaattgt tttataagat
    10321 atattccgat atttcaccgt ctttccgata tgcggctccg ggcaactttt gtttcagtat
    10381 ttgaattttc attagactga atacgccgtt tgaacggcac ggcgaaaacc cgggggatgc
    10441 cggacgttca gtcctttttc gcaccttgaa ggtaaagcat ttccaaggca attgtggcac
    10501 cggccaaagc ggtaatgtcg gattggtcgt aagagggggc aacttctaca acatccatac
    10561 cgacgatgtc gagatccgtc agcccacgta ggatttttaa tgccctgtcg ctgctcaagc
    10621 cgccgcatac gggcgtaccg gtcccggggg cgaacgacgg gtcgaggcag tctatgtcga
    10681 aagtcaggta aacgggcata ttgccgacgg tttctttgat tttacggacg gtctcttcaa
    10741 cactgtcttc attgactttg ggggcggaca acacagtaaa aggcaatttt ttactgtgtt
    10801 cggtgcgtat gccgatttgt acggaacggg acgggtcgat gaggccttcc ttgggggcgg
    10861 tatagaacat cgtaccgtgg tcgtattcgc tgccgttgtc gtaggtgtcg gtgtgcgcgt
    10921 caaaatgaat cagtgcgagt ttgccgaaat agcgggcgtg ggcgcgcaac aacgggaggg
    10981 taatgaaatg gtcgccgccc aaactcaaac agcgtttgcc ggaagaaagt aatttgccgg
    11041 cgtgcgcttc cattttttcg acaaaatccc tgctgtcgcc aaaagaaaaa accaagtcgc
    11101 cgcaatcaat aatgttcagg cgttcgcgca catcaaatgt ccatggaaac ctgcggtgct
    11161 cccaagcgag gttgacggag gcgcgccgga tggcttcagg accgaaacgc gcgccggaac
    11221 gccctgaaac cgccatatca taaggcacac cggtaataac ccaatcggca tgactttcat
    11281 acggcataaa attaagcggc aggcgcaaaa acccgaaatt attggaaacg agggagttgt
    11341 cggtttgtcc tgccagtgtg ctgtattgca tcgtaatgat tccttgtaat tggtttcaat
    11401 cggtcgtgat gattggtgtt tgagtaagaa aatcgggctt cagacgacat atccgatgcc
    11461 ttgatgcgtc tattcgtctt ccaaataggt ataaccatta agccccgctt cgagttcttt
    11521 taagaaagac atagcctgcg aggcaggaag gtctgaatgt tcgatttgtt cgcgatagcg
    11581 tttcatcagc tctttcggat cttgataaac gtattcgagc atatcggcaa cggtgtttcc
    11641 ttcatcgtaa tcgatgacgg taaattgtcc gtcttcccct acaacaacat cggcagtggc
    11701 agtgtcgccg aaaagattgt gcatattgcc gagtatttcc tgatatgctc ccaccataaa
    11761 aaagcctaaa agcggcggct cttcttcggg ataatcaggc ataggcatcg taccggcgat
    11821 gccgtcgccg tcgatgtagt ggtcaatcgt accgtctgaa tcgcaggtaa tgtccaacaa
    11881 cacggcgcgg cgcgcaatcg gttcattcaa accggtaatg ggacaaacag ggaaaagttg
    11941 atctatgccc caagcatcgg gcaaagattg gaagagtgag aaattgacat acagcttatc
    12001 ggcaaaacgt tcttgcaatt cgtcaataat ggttcggtga gaccggtgtt tttcattaaa
    12061 caattcgccg acttcatgac agatatttaa atacagttgc tccgcccacg cacgttgcgc
    12121 caaactcaac agcccgacat tatactgatt atgcacatcg gcaagatcaa actgcccttc
    12181 gtgtatccag ctgcgtaagg aacgtttttc ccgcgaggcg gaaatatccg tccaagtttc
    12241 ccacatactg tgcaacacac gcggcgcttc gggcgatggc gcatccagcc ggcgcggttt
    12301 gtaacgctca acgcctataa cattagcaac caaaacggcg tgatgtgcgg taatgccgcg
    12361 cccgctctcg gtgatgattg tcggatgcgg cagcccgtgt tcgagacaag cctgactgat
    12421 gccccatacg actgtggcgg catattcgtt gaggctgtaa ttaacggaac aatccgattg
    12481 tgtgcggttt ccttcgtaat ccacgccaag cccgccgcct acatcaaaac agcggatatt
    12541 tacccccagt ttgtgcaact caacataaaa ccgagccgat tcgtgtacac ctgtggcaac
    12601 atcacggatg ttcccaagct gcgagcccaa atggaaatgc aaaagctgca ggcaatccag
    12661 cctgtttttt tgtttcaaaa tatcgaccag ttgcaaaact tgggaagccg acaagccgaa
    12721 ttttgatttt tccccacccg aagactgcca ttttcccgaa ccttgggaag ccagtctggc
    12781 gcgcacaccc aaacggggct tgatgccgag tttttccgcc tcttccaata ccatttgtat
    12841 ttcggacagc ttctcaatca ccaaataaac ctgatgcccc agtttttcgc ccatcaaggc
    12901 gaaacggata tattcacggt ctttatagcc gttgcagacg attaatgttt gccgggtgcc
    12961 ggcgtgtgcc aaaaccgcca tcagttcggc tttagaacca gcttccaaac catgcggttg
    13021 tccgcttgac ataagcgatt cgatgacgcg gcggtgttgg ttgaccttga tagggtaaac
    13081 caaacaataa ccgcccttat agccgcactc ttcccgtgcc gtctgaaagg cgcggttaat
    13141 gtcgcggagg cggtgttcga ggatttgcgg aaaacaaaac aaaacaggca ggcgcgcctg
    13201 atgtttttgt tgcacggctt cagtcagttt ttgcagtgaa acagtttgat tgtgttgcga
    13261 gggattgggg cagacgatga tttcgccgga atcgtcaaca tcataataac ctatgcccca
    13321 atgattaatg ttgcacactt cacggatggt aaggataggc ataataaacc tgctccgtct
    13381 gtcgtgttga aaaggaatga ttataacaaa tcagcgtgaa atgtcatttt tttaataaga
    13441 aaagcctgcc tcatacctga tgaggaacag gcaaaatgtc gtctgaacgc ttcagacggc
    13501 attttggttc atctttccat caaaggagtc agcgatcgag ctgctctttg atgattttca
    13561 ggtcggggta agacaacacg atgtcgtatt cgcggccgtc tttataggct tccacttcca
    13621 aaacaggttt gccccaatgg tcgtcggcat cgacatcgtg aacctgataa ccgcgctgtt
    13681 ccaacatttt tacggctttt gtgcggtttt gttcaaaatg gggatcaccg taaatctgac
    13741 gctcggcaga atcgtcggca aatgcggttg cggcactcag ggaaacaacg gcagccaata
    13801 acagtttttt cattttcagt cctttcttaa tcggttgatt gaataagatg tgtttttcaa
    13861 tgctgccatt aaaacacagc aaaattaggt ttgaattagc aggaagttaa gaagcgtaaa
    13921 tgccgtctga aaaaaacaga gccgtcaaac ggctccgttt tccttatgct tctttggttt
    13981 cagccgcctt ttgacgcagg cgcaagctca attcgcgcaa ctgcttgtcg tccacgctgt
    14041 tgggcgcgtt ggtcagcagg cattgggcac gttgtgtttt cgggaaggca atcacgtcgc
    14101 ggatagattc ggcgccggtc atcagggtaa ccaaacggtc gaggccgaat gcaaggccgc
    14161 cgtgaggagg cgcaccgaat ttcaggttgt ccaagaggaa gccgaatttc tcttgttgct
    14221 cttcagggct gattttcagc gcggcaaaca ctttctcttg tacgtctgca cggtggatac
    14281 ggatagagcc gccgccgatt tcccagccgt tcaataccat gtcgtaggca cgggccaggc
    14341 agtttgcagg gtcggaaacc atcaggtctt catgaccttc tttaggcgcg gtaaacggat
    14401 ggtgtacggc aacgtagcgg tcggcttctt cgtcgtattc gaacattggg aaatcgacaa
    14461 cccacaaagg tttccattcg tctgtgaagt agccgttgtc tttgccgtgt tccaagccga
    14521 ctttgatacg caatgcgccg atggcttcgt tcacaacttt ggttttgtcc gcaccgaaga
    14581 agataatatc gccgttttgt gcgccggtac gctcgatgat ttctttcagg gcattctcag
    14641 acaggaattt tacgattgga gattgcaggc cgctgtcttc gccgtttgag aggttgccca
    14701 catcgtttac tttgatgtat gccagacctt tcgcaccgta gatgccgaca aatttagtgt
    14761 attcgtcgat ttctttgcgg ctgaatttcg caccgttcgg cacgcgcagg gcaaccacgc
    14821 ggccgccttt catatcggct gcgccacgga agactttaaa ctcttccgtc ttcatcaggt
    14881 cggtcaactc ggtaaatttc aagccgatac gcatatccgg tttgtcagaa ccgtaataga
    14941 acatcgcttc ggcgtaaggc atacgcggaa aaccgcccaa gtccacgccc agcgcgtctt
    15001 tgaagacttg tttcgccata ccttcggtga tgtccatgat ttcatcctcg tttaagaacg
    15061 aggtttccaa gtcgatttgg gtaaattcgg gctggcggtc ggcacgcagg tcttcgtcgc
    15121 ggaagcactt ggtgatttgg tagtaacggt cgaaacccgc caccatcaac agttgtttga
    15181 ataattgcgg cgattgcggt agcgcgaaaa actcgcccgg atgaacgcgg ctcggcacga
    15241 ggtagtcgcg cgcgccttca ggcgtggagc gggtcagcat cggggtttca atgtcgatga
    15301 aaccttgcgc gtccaagtag cggcgaacgc ccatagcaac ttggtaacgc aggcgcaggt
    15361 tgcgctgcat caccggacgg cgcaggtcga taacgcggtt ggtcaggcga acgttttcgc
    15421 tgatgttttc gtcgtcgatt tggaacggag gcgtagcggc agcgttcaac acttcgattt
    15481 ctttggcaag gatttcgatt ttgccggaaa tcattttgtc gttggtcgtg ccttcgggac
    15541 ggttgcgtac gcggccggta atgctcaaaa cgtattcgtt gcgggcggag tcggcagcgg
    15601 caaatgcttc gggcgtgtca gggtcgataa cgacttgaac gatgccttcg cggtcacgca
    15661 ggtcgataaa aatcacaccg ccgtggtcac gtcggcggtg tacccagcct ttgacggtaa
    15721 cggtttggtc taagtattgc tcgctgatca ggccgcagta gttggtacgc ataaaatcac
    15781 cttttattga tttaaactga aaacagaaaa tgccgtctga acggcggctt tattgttgtt
    15841 cgggcaaatc cgccttttca gacggcatag gtcctgccaa tgttttgacg ggcaggtcgt
    15901 cagggatgac catacccagc gaaatcacat atttcaacgc ttcgtccacg ctcatatcga
    15961 gttcgcgcac atcgcttttc tttaccataa tatagtaacc gccggtcggg ttgggcgtgg
    16021 tcgggacata cacggaaaga taatcgccat cctgcggcaa tgcggcctta accgcattcg
    16081 acacctgccc tgacacgaaa gcaatcgtcc aaatacccga ttggggaaac ggcacgagta
    16141 ccggcgtttt aaacgagcgg ctgctgtcgg acagcagcga ttcggatact tttttcacac
    16201 tcgagtagat ggatttgaca accggaatcc gccccaacag gctgtcccac gcggcaagaa
    16261 tctgccggcc caatacgttg gcggcaaata atccggttac aaacaatacg gcaatggcaa
    16321 caataacgcc cagccccggg atattgaagc ccaaaacata ttgcggccgc cattgcttcg
    16381 gcagcaggtt gacgagctga tcggacgcgg aaacgatata ggaaaccacc caaaccgtta
    16441 ccgcaatcgg cagccagacc aaaatgcccg taatcagata tttttttaac gccttggcag
    16501 ctttgccgcc ttcggccgca ggttccgtca tcttgcttga ttccgacaaa gtccgtacaa
    16561 accgcacatt atacgcgttt gcccggattc aaacgaaatt tttatcccgc cccgccaaac
    16621 cgccggcggc ttcagacggc acggcaactt gatatatcgt ctgaacacgc ggttcagatg
    16681 ccgtccaagt cgttgaacat caacccgata ccgataccgt tctgcttgtg gttgtagtcg
    16741 atcaggctct cgccgtaacc gtggaatccg cgtaccacgc ctttgagttt gcccttaatc
    16801 ggaaacgtgt aggcggcttc aatcgcgccg tagcccgttt tggggttgta gcgcaatacg
    16861 gaatacacat tctgcctgtc gttcaggcgg tactgcagct tcacgtcgcc ataccccata
    16921 tagtcggcaa tatcgggatt gtcgttttta tcgccgctct gatcgaacgc acgcacccac
    16981 acgcgcggaa tcaccgtcaa tttgccccat tccatgcctg ccatggcata aatcctgttc
    17041 cacgaacgcg attcgggacg gctctgtccg ttggactggt ggacaaaacc cgcaccgagc
    17101 atacgcagcc tgccgccgaa cggcaaatct gccttcacag gctgggtcag gaaaatttca
    17161 ggtttgtaat ccgtattgcg gaacggcgcg gatttcctgc cttggttgta aatctgccaa
    17221 tcggatcttt gggtgtagcc gaaccacaga tccgcgcggg ttttaaacaa atcttcggca
    17281 attttgcttt tgaacgaaac ctgcaatttg gtttccgcac gtttctgctg tccgaatttt
    17341 tcctgtacag tcgtaccgcg cgtcggcgaa cccggggcat agttgggcga attgttgtac
    17401 cagagcggca taaggtacat cggattgtgt tcgcgtacgc ccaacagccc gcgcaaatcg
    17461 tttttgtcca agtcgtacat caggctcaaa ggcgtataga tgtcggcggt ttcgcccgca
    17521 ctgtcggcag gaagcgcatc cccgcctttt tcaacaacaa tgaccgcctc gcccttatcc
    17581 aggctgctgc ggacggtttc cgtcagattg agtacggctt tcgactcctg cccttcctgc
    17641 cctgccgaag acggaagctg tgccgcaaaa atcctgtcgt aacacgccaa acgcgtaaca
    17701 ttgtccgtca aagcggcgca ttgcagcgcg gtctctccaa aagcggatgc catcggcaac
    17761 agtcctgtca aaagaatata gcgcatattc cgtgtattca tctccgcccc cattgtcggc
    17821 atattggttt tcaaacggca ttttatagcg gattcggata aaaaccgcac cctttccgcc
    17881 atttcgggat tttgcccccg caatacagaa aaccccgaaa ccgtcgggct tcagggtttt
    17941 ccgcttatcg cgtatcaacc gccttggcgg ttttgcaaaa atcaagccag tgcttttact
    18001 tttgcagaca gacggctttt gtggcgtgcc gctttgtttt tgtggaacac gcccttgtcg
    18061 gcgatgcggt cgatgacttt gacagactct tggtaaaccg cttgtgcggc agctttatcg
    18121 cctgcttcga ctgctttcaa tacttttttc actgcagtgc ggaatgcggt acgcaggcta
    18181 gcgttgtggg cgcgttgttt gacggactgg cgggcacgtt tgcgtgcttg tgcgctgttt
    18241 gccatattgg aatctcctga aagaaaaata aattctgtaa acgcgcaatt ttaaaggggg
    18301 tttacattat atgcaagcct tttctctcta tccgcccgtc ggacaccctg acagcgcaaa
    18361 aaaacggcaa aatcgtgcgg acgttttctc cctcttttga aaacaaggta attttttagg
    18421 aaaatcaggt caatatcgct ttgagtttgc acaaatttac tttacaatgc ccaaaccttg
    18481 cgccggggtg tttatggggc gcggggtttt ctttttttcc aacctctttc atcaggaatc
    18541 tgcccgtatg aaaaaatccg tattagccgt tttggccgcg ctgtctctgg cagcctgcgg
    18601 cggcagcgaa aaaaacgccg tgcaacctca agcagacgct gcgtctgctg ccaatgccga
    18661 agccgctgcc accgatactc tgaacatcta caactggtcg aactatgttg acgagagtac
    18721 ggtcgaagac tttaaaaaag ccaacaatct gaagctgact tacgacttgt acgaaaacaa
    18781 tgaaacgctg gaagccaaaa tgctgaccgg caaatccggt tacgatttgg ttgtgccggg
    18841 catcgccttc ctgccgcgcc aaatcgaggc gggcgcgtat caaaaagtca acaaagacct
    18901 gattcccaac tataaaaaca tcgatcccga actcttgaaa atgctggaaa ccgccgaccc
    18961 gggcaaccag tatgccgtcc cctatttctc cggcgtgaac acgattgcga ttacggcgaa
    19021 aggcaaagag cttttaggcg gcaagctgcc cgaaaacggc tgggatttgc tgttcaaacc
    19081 tgaatacacc cacaaactga aatcctgcgg catcgccctg tgggacaccc cgagcgaaat
    19141 gttcccgatt ttgctgaact acttgggcaa agaccccaaa ggctcgaatc ctgaagattt
    19201 gaaggcggcg gcggaagtgt tgaagtctat ccgtcctgat gtcaaacgtt tcagcccgtc
    19261 catcatcgac gagctggcgc gcggcgacat ctgcctggcg gcaggcaacg gcggcgattt
    19321 gaacttggcg aaagcacgtt ccgaagaagt gaaaaacaac gtcggcatcg aagtgctgaa
    19381 tccgaaaggc atgggcttct ggattgagtc ttggctgatt cccgccgatg cgaaaaacgt
    19441 cgccaatgcc cacaaataca tcaactacac gctcgacccc gaaatcgcgg cgaaaaacgg
    19501 catcgccgta accttcgccc ccgccagcaa accggcgcgc gaaaaaatgc ctgccgaact
    19561 ggtgaacacc cgttccatct tcccgaacga gcaggatatg aaagacggtt tcgtgatgcc
    19621 gcaaatgagt acggatgcga aaaaactgtc tgtcagcctg tggcagaaaa tcaaagtcgg
    19681 caccaactga tttgaagcat taaaaatgcc gtccgaacat cgttcggacg gcattttata
    19741 ttggattgaa atagaaatat ttatatagtg gattaacaaa aatcaggaca aggcgac
//
LOCUS       DAWFBO010000023        19544 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFBO010000023 DAWFBO010000000
VERSION     DAWFBO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.023"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(242..844)
                     /locus_tag="WGO87_000570"
     CDS             complement(242..844)
                     /locus_tag="WGO87_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0391964.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQAMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(878..2149)
                     /gene="purD"
                     /locus_tag="WGO87_000571"
     CDS             complement(878..2149)
                     /gene="purD"
                     /locus_tag="WGO87_000571"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0391965.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSATAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            2307..2624
                     /locus_tag="WGO87_000572"
     CDS             2307..2624
                     /locus_tag="WGO87_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0391966.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            complement(2676..3287)
                     /locus_tag="WGO87_000573"
     CDS             complement(2676..3287)
                     /locus_tag="WGO87_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0391967.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(3350..4285)
                     /locus_tag="WGO87_000574"
     CDS             complement(3350..4285)
                     /locus_tag="WGO87_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0391968.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            complement(4296..5045)
                     /locus_tag="WGO87_000575"
     CDS             complement(4296..5045)
                     /locus_tag="WGO87_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0391969.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            complement(5272..6240)
                     /gene="waaC"
                     /locus_tag="WGO87_000576"
     CDS             complement(5272..6240)
                     /gene="waaC"
                     /locus_tag="WGO87_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0391970.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEVGFTDI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEVGRLKNLAQPYYAALHATSRDSKLWPMENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            complement(6278..6913)
                     /locus_tag="WGO87_000577"
     CDS             complement(6278..6913)
                     /locus_tag="WGO87_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0391971.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGNWQVIVNEAACRGIAPDTIETAWQEMSSAGAVILKNAEEIKKYINNQ"
     gene            7027..7707
                     /gene="tsaA"
                     /locus_tag="WGO87_000578"
     CDS             7027..7707
                     /gene="tsaA"
                     /locus_tag="WGO87_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0391972.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLASAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIIDIKPYIPFVESKPDASSGFVSGKPEELEVV
                     WLENIGAENLSESTQNLISQSIAQDPRPAYQDIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            7776..7895
                     /locus_tag="WGO87_000579"
                     /pseudo
     CDS             7776..7895
                     /locus_tag="WGO87_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7965..8969)
                     /gene="gap"
                     /locus_tag="WGO87_000580"
     CDS             complement(7965..8969)
                     /gene="gap"
                     /locus_tag="WGO87_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0391973.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQNILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            9411..12005
                     /gene="mutS"
                     /locus_tag="WGO87_000581"
     CDS             9411..12005
                     /gene="mutS"
                     /locus_tag="WGO87_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0391974.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRHIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TTQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            complement(12128..12619)
                     /locus_tag="WGO87_000582"
     CDS             complement(12128..12619)
                     /locus_tag="WGO87_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0391975.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(12623..13018)
                     /locus_tag="WGO87_000583"
     CDS             complement(12623..13018)
                     /locus_tag="WGO87_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391976.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNTVYGAGGTKASGN"
     gene            complement(13039..14433)
                     /locus_tag="WGO87_000584"
     CDS             complement(13039..14433)
                     /locus_tag="WGO87_000584"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0391977.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRRFDRYKEVIAELLEKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            complement(14549..15205)
                     /locus_tag="WGO87_000585"
     CDS             complement(14549..15205)
                     /locus_tag="WGO87_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0391978.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIDSVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            complement(15202..15555)
                     /gene="arsC"
                     /locus_tag="WGO87_000586"
     CDS             complement(15202..15555)
                     /gene="arsC"
                     /locus_tag="WGO87_000586"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0391979.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIAEHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            15679..16161
                     /locus_tag="WGO87_000587"
     CDS             15679..16161
                     /locus_tag="WGO87_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0391980.1"
                     /translation="MKYLNLAAIALAATFAAHTASADELAGWKDGTAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            16355..17005
                     /gene="ftsE"
                     /locus_tag="WGO87_000588"
     CDS             16355..17005
                     /gene="ftsE"
                     /locus_tag="WGO87_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0391981.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            17002..17919
                     /gene="ftsX"
                     /locus_tag="WGO87_000589"
     CDS             17002..17919
                     /gene="ftsX"
                     /locus_tag="WGO87_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0391982.1"
                     /translation="MSIIHYASLHIESARTALKELLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRIANVRFIGKEDG
                     LAELQSNLDQNLVSMLDGNPLPDAFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSTVRPLVDAIFKPYGLNIGWRFFYPEEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            18010..18258
                     /locus_tag="WGO87_000590"
     CDS             18010..18258
                     /locus_tag="WGO87_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0391983.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(18325..19503)
                     /locus_tag="WGO87_000591"
     CDS             complement(18325..19503)
                     /locus_tag="WGO87_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0391984.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASIASV
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
BASE COUNT         4919 a         5475 c         4415 g         4735 t
ORIGIN      
        1 aatcatcttc ggcgttttca tttttatgga ttaaaacaac acgggaaaaa tctgttttca
       61 gatgattgcc cgcttgattg ttcggattat tgtccggaac gacaaaaccg tcctcaaaat
      121 taaagcagac gttgcgtcct tctaccttta tctctgtgca ataacaatca tgtagagaaa
      181 tgctatccga aaaatttttt ctttgtgtat gcaaaaaaag ttttcattca agtacccata
      241 tctaacgcaa acgtttacct gtttccccgt caataatctg actcggcgat ttctgcctgc
      301 cgattcgccc accaacaatc cacacatcgc gtccgaattg ccttctgact tccctctccg
      361 tccgacacgc gcgtttgcct gcgcggttgc acgaagtcga gaccaaaggc gtttgcaaag
      421 cctgacacaa gcggcgcgca cctacatggg cgggaaccct gaccgccaac ttgctgcgct
      481 gtttcccgcg taactcaggt aagacacagg atttggcaga taataggaac gttttagggg
      541 cgggccattc ttttctaagc atagcctgca gattttcaga cggcatttga agtaaaggct
      601 gcaattgctc aaattgattc ccgatgacaa tcataccctt gtgttgcggt ctttttttca
      661 aatgcgccaa cttaccgagt gctttggcta atgtcggaag acaccccaag ccataacaag
      721 attcggtcgg ataagcgacc aaaccacctt ttttcaaata aacgcttaac ttacgttgcg
      781 ctgatgctgc gataattctc ggaaataaca taatataaaa taccgtctga agcacattag
      841 tcatacttgg cttcagacgg catcatcctc tttctaatta acggttaatc gctttatcgg
      901 caatgtcttt acggtattgc atcccgtcga aactgatttt ttccaacgcg ccatatgcct
      961 ttgctttcgc ttgcgccaca ttgtcgccca atcccacaac acacaatacg cgtccgccgt
     1021 tggtcaatac gtcacctttc tcgtttgccg ttgtacctgc atggaaaact ttgcctactt
     1081 ggttggcagc atccagaccg gaaataacat cgcctttttt gggcgtttcg gggtaatttt
     1141 gcgctgccag taccacgccc acggcagttt gcgggctcca ttccgcggtt gcgctatcga
     1201 gtttgccgtc tattgccgct tcaaccaaat ccgataagtc gctgttcagt cggctcataa
     1261 tcggctgggt ttcaggatcg ccgaaacggc agttaaactc aatcgtatag ggtgcaccgc
     1321 tttgatcaat catcaaacct gcgtacagga aaccggtgaa ctcatgcccc tccgctttca
     1381 tccctgctac ggtcggcaaa ataatttcat tcatcgcgcg ttcgtacaca acaggcgtta
     1441 ccacaggcgc agggctgtac gcacccatac cgcccgtatt cagacctttg tcgccgtcta
     1501 aaagacgctt gtggtcttgg ctagttgcca taggcagtac attattgcca tcaaccatga
     1561 cgataaaact cgcttcttcg ccttgcagga aatcttcaat cacaacacgc gcgccggcat
     1621 tgcccatttt gttgtccagc agcatatcat caatcgcagc atgcgcttca tccaaagtca
     1681 tcgccacaat cacgccttta cctgccgcca aaccatcggc tttgataacg ataggcgcac
     1741 ctttctgatt gacgtaatca tgtgcggcat cggcgttttc aaaggtttga tattgcgcgg
     1801 tcggaatgtt gtatttcgcc ataaatgctt tggcgaaatc tttggaactt tccaactgcg
     1861 ccgcatattg tgtcggaccg aatattttta gtcctgcggc acggaaatca tccacaacac
     1921 ctgccgccaa aggcgcttca gggccgacga cggtaaaaac aatattttct ttacgacaga
     1981 attcaatcaa atcctgatgc gcagtcaagt cgatgttttg caacttgggt tcaatcgctg
     2041 taccggcatt accaggcgca acaaatactg tttccacttt aggcgactgc gccaatttcc
     2101 aagccagcgc gtgttcgcga ccgccattac cgataaccag cagtttcatg ccatctcctt
     2161 gacaaatatg tacttttaac gaaaactcga tacaaaggga cttttatccc atctgaagaa
     2221 attttagtag aatcaaacaa aagaccgctt cattccactc tgcaacctat tcaacttatc
     2281 cataaattaa aaaaggacaa gcaaccatgc aaaaacgtat tgatgaaatc caaagcaaat
     2341 accgcgaatg gtgtcattta ctaccgcaac tggaagaaga catccgccgt tggaaatatg
     2401 tcgtcacttt aattcgcgac atggacaatt tctataccca cgagtatcag gcgtgtcatc
     2461 aggctattga agacggggta gaactggatt tgagtacgga aggcgaatac agcattatga
     2521 gtgaagatgc actatggaac gcgctgggcg aattccatca attggcttgg ttatatttgc
     2581 gctccagcgt cgatgcctta gacaaatata cacaagaaga ttagtcagcg aagaggtcgt
     2641 ctgaaatacc atcacaaagc atttcagacg acctttcatt caaaaggctt ttccgtattt
     2701 acttcaatct gccgagtatt cttccaagcc gcaacacagg cctcataatt taccaacgac
     2761 aaactgaccg tcaatcggca atccaactgc aaatcccgct ccaatatatc cgcctgatat
     2821 tgtttggcaa tgcgtatcgc ttcattcaaa aacggatatt cacatttcag ccaaacagtt
     2881 ttttcaatat tcttttcaac tacttctgca actgccaacg cttgagccgt cgcctctttg
     2941 tacgcatgta tcagacctgg aacacctaac aaagtaccac cgaaatagcg gacgaccaca
     3001 accaaaacgt cggtaatacc caccgaatca atctgtccca aaattggtcg tccagcactt
     3061 cctgatggct ctccatcatc gttggcacga aattgcatgc catccacacc caaacgatag
     3121 gcatagcacc agtgtcgtgc tttatgatgc tcttccttta acggatcgag gtattttttc
     3181 acatcagcca atgtccgaat cggataggca aatgcaataa aacggctgcc tttatcttta
     3241 aactcagcct gcgtcgagga agtaatggtt ttataagtcg taatcatgct gaaatgtttt
     3301 cagacgacct cattaataac aaggtcgtct gaacgtttca cgtgaaacat taatttttca
     3361 atacttctgt taattgtgga acgatttcaa ataaatcgcc aaccaatccg taatcggcta
     3421 cattgaaaat cggcgcatca gcatctttat tgattgcaac aatcacctta ctgtcttgca
     3481 taccggcaac gtgttgaatc gcacctgaaa taccgattgc aaaatagagt tgcggcgcaa
     3541 ctactttacc ggtttgtccg acttgagcat cgtttggcgc atattcggca tcaaccgccg
     3601 cacgggatgc accgattgcc gcacctaaaa catccgccaa cggtgtcagc acttcattga
     3661 atttttccgc actacccaac gcacgaccac cggaaacaat cacttttgcc tgagtcagtt
     3721 caggacgatc ggaatgggaa agctgacggt taacaaaacg actcaggttt tgggcagggg
     3781 ttgcttcaac attaattacc tcagcattac caccttgcgc cgccactgcg tcaaaaaccg
     3841 tcgcacggaa ggtcagcacc aatttttctg aatcagcttg cacggtttca aatgcattac
     3901 ccgcataaat ggggcgcaca aaagtcgtgt tatccacaat ttcggtcaaa tcagaaattt
     3961 gcggtacgtc taataaggct gctacgcgag gcaaaaggtt tttaccgaat gtagttgccg
     4021 ttgctgcaac atagcagtaa tccgctgcca atttaacaac cagcggagcc aactcttcag
     4081 ccaaaccttc ggcataatgg gcagcatctg caaccaaaac tttttccaca cccgccactt
     4141 gttttgcgaa ctctaccaca gcagatgcac cgcttccggc aaccaataaa tcgattttac
     4201 ccagcttagc ggcagcggta acagcatgca aagtggtagg attcaactgt ttgttgtcgt
     4261 gttcgacaat aatcaataca ctcatttcag cctcctcaaa tcactttggc ttcgtttttc
     4321 aatttttcaa ccaattcggc aacgcttgct acttttacgc ctgcctgacg cgccttaggt
     4381 tcggcaaatt tcaccgtttt caaacgaggt gaaatgtcgg caaccaaatc gtcaggagtc
     4441 agtttttcca aaggtttttt ctttgccgcc ataatattgg ggagtttgac aaagcgcggc
     4501 tcgttcaaac gcaaatccgc gctgataaca gcaggcagtt tcaatgcgat ggtttcttcg
     4561 ccgccatcaa tttcgcgcac aatctgtact tcaccacctt caatgtgtac ttttgaagca
     4621 aacgtacctt gcgccgcatt cagcaaagct gccagcattt gcgccacttg attggcatca
     4681 tcatcaatcg cttgtttgcc caaaaagaaa atttgcggat tttctttgtc cgcaacggct
     4741 ttcagcaact tagcaacggc cagagactcc agtttagtat cggtttcaac atgaatggca
     4801 cggtcggcac ccatcgccaa agctgtacgc aacgtttctt cgcatttctt ttcgcccaaa
     4861 gaaaccgcta cgatctcgct tacttttccg gcttctttca aacggacggc ttcttccaca
     4921 gcgatttcgt caaacggatt catcgacatt ttgacattgc cgatatccac atccgaacca
     4981 tcggctttta cacgaacttt gacgttgtag tccactacgc gctttactgc gaccagtgct
     5041 ttcattgaac cctcctaaaa agaacgctgc tttcaccatc cagcgaaacc aaaccttctt
     5101 ccctataaaa ccaaatccgt tttccttaaa aacgaattca ttcaaaaatc tttcggataa
     5161 tgcttgccga ttataccatt tttaaagcat ttactcagac tagcggatat acattcctgt
     5221 atctaataaa ttggaaaata tcatgccgcc atatcagttt tagacgaccc tttagccttt
     5281 atctgctgca acacaatcca tcagcgcttg ataaaccaaa tctgcggtcg gaatctgccc
     5341 gatattgccc aaattttttg caattggcga aacctgaacg cctgttttaa tcggatcggt
     5401 atcggtataa atgccgacca caggtttttc caaggcattt gccaaatgca gcaaaccggt
     5461 atccacgccg acaattccga ccgcgtattt cagcagatac gctgcctgca ataaatttat
     5521 tttgtcgcac acaatagcaa acggcagtcc gtctgcaatc tgtttggcac gcgttttttc
     5581 atcttcattt ccccaaggca ggtaaatatt gcattgctgt tcttcattca acttttgcag
     5641 caacgaccgc cagttttcca taggccataa cttactgtcc cgactggtcg catgcaaagc
     5701 cgcataatac ggctgcgcta aatttttcag acggcctacc tcaggaacag tcaagccaaa
     5761 tacctgcgtt tcaggcatcg catacccaaa tacttgggcg aacagctcac gattgcgcca
     5821 aacagcattt ttccctttcg gtacagcgta tgttttcgca tacgccagcg cagccagtcc
     5881 ctcgcgcgcg ctatttttat ccaaaccata aataggggat ttcgccattt tagcgaaaca
     5941 cgcgctttta atcagacctt gactgtccaa tacaaaatta aatgcttcct gccgcaaagt
     6001 ctgtttcaga tgacccattt cccgccaagt ttcagcctga aagagatgtt tgcgccattg
     6061 ccgccatttc atcacatgga tttttttcac aaacggatgc aggcgcgcaa tatctgtaaa
     6121 tccaacttca cacaaccaat ggagttctac atcagggcat tgtcgcgcca aatcctcaat
     6181 tgcgggcaaa gtgtgaatta aatcgcccat actagacaaa cggacaagca aaattttcat
     6241 attttaagaa gtatgtttca cgtgaaacaa ttttaactta ttgattatta atatattttt
     6301 taatttcttc agcgtttttt aagatgactg caccggcaga actcatttcc tgccatgccg
     6361 tttcaatggt atccggcgca atgccccgac aagccgcttc attgacgata acctgccaat
     6421 taccaccttt aagtaactgc aaaaccgttg ttttaacaca ataatccgta gctaacccac
     6481 cgataataac cgtatccgta ttttgacaac gcagccattc aatcagccct gtgcttagtt
     6541 tttcctcaat atcgtgaaaa cacgcgccgt aaggatgcaa ttcaggatca acacctttcc
     6601 aaacgcaata atcatattct ttggcagaag gcagtccgtc caataattca tagccgcgcg
     6661 taccgaccat cgcatgagcc acccaagtca aatccgcatc aggcaaacct gtcggcttca
     6721 acatatcaac agggttatcc acaagccatt tcgctaccat atgatgcgca tctttcgtca
     6781 tcacgcgcaa atccgccaaa gcggcttgag cattcaactc ctcgacaatc aaatgcccct
     6841 cgttcacggg cagttcgtca ggacacagtg gcgtaaacgt tttttgtgca tcaacatcaa
     6901 tggaaacaat catctcatta tttcaacgcg attaaaatgc cctgtattat aacaaattac
     6961 tgcccaaaag cggtaaaacc gattgtgata agataaggtt tttccaaaaa acttacccac
     7021 aaccttatga cttataccat tacccccatc ggcaccgccc gatcgcccta caaacagaaa
     7081 ttcggcatcg cccgccagcc cggtttagcc tctgccgcag aagtatgcat cgagctgaat
     7141 cccgaattta ccgcagacag cgtgcgcggg ctggaagatt tcgattatgt gtggataagt
     7201 tttatttttc acggcgtatt ggatgaaggc tgggcgcaaa tggtgcgccc gccacggctc
     7261 ggcggcaaac aaaaaatggg cgtgttcgcc acgcgcagcc cccaccgccc caaccatctc
     7321 ggactctcgc tcctgaaact cgaacgcatc gaaaccggca aacccgtccg cctctattgc
     7381 agcggcgcag acctgttgga cggcacacca attatcgaca taaaacccta catccccttt
     7441 gtcgaatcca aacccgatgc atcatccggc ttcgtcagcg gcaaacccga agagcttgaa
     7501 gttgtatggc tggaaaacat cggcgcggaa aatttatctg aaagcaccca aaacctaatc
     7561 agccaaagca ttgcccaaga cccgcgcccc gcctatcagg atattcccga acggatttat
     7621 gtgatgaata ttgcggatta cgaagttagg tttcaaatcg aagaaaaccg tgcaaccgtt
     7681 attgatcttt ccccaacccc gctttaaatc gggcaaaaat ccggttttgc cgcatagcag
     7741 ttgaacaaac ggctgttgtt tgttcgccat aagccgcaat atcaagttat agcggattaa
     7801 atttaaatca ggacaaggca acgaagccgc agacagtaca aatagtacgg caaggcaagg
     7861 taacgccgta ctggtttaaa tttaatccac tatacagata aacaatgccg tctgaacgca
     7921 atgtgttcag acggcattta cttatccaca ggtttgttca agccttagat tttgcctgca
     7981 aagtattcca aagtgcggac gagttggcag gtataggaca tttcgttgtc gtaccaggca
     8041 acggttttca ccagttgttt gccgcccacg gtcatcacgc gggtttgggt cgcatcgaag
     8101 agcgagccgt attcgatgcc gacaacgtcg gaagaaacga tttgatcttc gttgtagccg
     8161 taagattcgc tggcggctgc tttcatcgcg gcgttgattt cttctttggt tacagggcgt
     8221 tcgaggacgg aaaccaattc ggtcagcgag ccggtggcaa cagggacgcg ttgggcggag
     8281 ccgtcgagtt tgccgttcaa ttcagggata accagaccga tggctttggc ggcaccggtg
     8341 ctgttgggca cgatgttgag cgcggcggcg cgggcgcggc gcaaatcgcc tttgcggtgc
     8401 ggcgcgtcaa gggtgttttg gtcgccggtg taggcgtgga tggtggtcat caggccttcg
     8461 actacgccaa attccttttg caggactgcc gccatcgggg caaggcagtt ggtggtgcag
     8521 gaagcggcgg aaatgacggt ttcgctgccg tccaaaatgt tttggttcac gccatatacg
     8581 acggttttca catcattgcc gccaggtgcg gaaatcacga ctttgcgcgc gccggcacgg
     8641 atgtgggctt cggctttggt tttgttggta aagaagccgg tacattcgag gacgacatcc
     8701 acacccaact cgccccaagg caattcttcg ggattcggat tggcaaaaac tttgatttct
     8761 ttgccgttta ccacgatggc atcgtctttt aattcggcag taccttggaa acggccttgc
     8821 gtgctgtcgt atttgaaaag gtgcagcagc atttcggcag gggtcaggtc gttgacggcg
     8881 acgacttcga tgtcgtgggc tttttcaatt tgacgcaatg cgaggcggcc gatgcggccg
     8941 aaaccgttaa tcgctacttt aatgctcata ttttgtctcc aggttgtgaa ataaagtccg
     9001 atgcctgcat tgtattctga aataaagtta cattccacta ttacatctaa ctacttgacg
     9061 cttatttgat atagatgaat tttgctgttt gcacagattt ccaaaacctt taccatcaat
     9121 atttgaattt aaattttaat gatgattttg atgattgcca acctgcttgt gcgtaagtag
     9181 caaatatcca atattttcat tatctttttg tcaaataagt ttgagtttaa gacttgctgt
     9241 ataagacaga taagcgtgga tgttttttga cttaataata tttctgtgga taactttgct
     9301 gttttcctag ttgtctccac aaccttattg acaggcttac ggtcagtctc attccgtcga
     9361 agacaaaacc ttttgctaca ataccgtttt cctaatgata aggcagcccc atgtccaaat
     9421 ccgccgtttc cccaatgatg cagcaatacc tcggcatcaa agcgcaacat accgacaaac
     9481 tggtgtttta ccgtatgggc gatttttacg agatgttttt cgacgatgcg gtagaagcgg
     9541 caaaactttt ggatattacc ctgaccacgc gcggacaggt ggatggcgag ccggtcaaaa
     9601 tggcaggcgt gccgtttcac gccgccgaac aatatctggc gcgcctggtc aagttgggca
     9661 aaagcgtggc gatttgcgaa caggtcggcg aagtcggcgc aggcaaagga ccggtggagc
     9721 gcaaagtcgt gcgcatcgta acgcccggca cgctgaccga ttccgcattg ctggaagaca
     9781 aggaaaccaa ccgcatcgtt gccgtgtccc ccgacaaaaa atacatcggt ttggcgtggg
     9841 catcgctgca aagcggcgaa ttcaaaacca agctgacaac tgtggataaa ttggacgacg
     9901 aactggcgcg cctgcaggcg gcggaaattc tgttgcctga cagtaaaaac gcaccgcaac
     9961 ttcagacggc atcgggtgtt acgcgcctga acgcgtggca gtttgccgcc gacgcggggg
    10021 aaaaactgct gacggaatat ttcggctgcc aggatttgcg cggcttcggt ttggacggca
    10081 aagaacacgc cgttgcgatt ggcgcggcag gtgcactgtt gaactatatc cgtctgacgc
    10141 aaaacctgat gccgcaacat ttggacggcc tgtcgctcga aaccgacagc caatatatcg
    10201 gtatggatgc cgccacgcgc cgcaatctcg aaatcacgca aaccctctcc ggcaaaaaat
    10261 cgccgaccct gatgtccacg ctcgaccttt gcgctaccca tatgggcagc cgcctcttgg
    10321 ctctctggct gcaccaccct ttacgcaacc gcgcccacat ccgagcgcgc caagaagccg
    10381 ttgccgcgct ggaaagccaa tacaaacccc tccaatgccg tctgaaaaac attgcggaca
    10441 ttgaacgcat tgctgcccgc atcgccgtcg gcaacgcccg cccgcgcgat ttggcatctt
    10501 tacgcgacag tctgttcgag ttggcacaaa tcgacttgtc cgcaaacggc agcagcctgt
    10561 tggaaaccct caaagccgtt ttcccggaaa acctatccac agccgaacag ctccgccaag
    10621 ccattttgcc cgaaccttcc gtctggctga aagacggcaa tgtcatcaac cacggttttc
    10681 atcccgaact ggacgaattg cgccacattc aaaaccatgg cgacgaattt ttgctggatt
    10741 tggaagccaa ggaacgcgaa cgtaccggtt tgtccacact caaagtcgag ttcaaccgcg
    10801 ttcacggctt ttacattgaa ttgtccaaaa cccaagccga acaagcacct gccgactacc
    10861 aacgccggca aaccctcaaa aacgccgaac gcttcatcac gccggagttg aaagcctttg
    10921 aagacaaagt gctgactact caagagcaag ccctcgcctt agaaaaacaa ctctttgacg
    10981 gcgtattgaa aaaccttcag acggcattgc cgcagcttca aaaagccgcc aaagccgccg
    11041 ccgcgctgga cgtgttgtcc acattttcag ccttggcaaa agagcggaac ttcgtccgcc
    11101 ccgagtttgc cgactatccg gttatccaca tcgaaaacgg ccgccatccc gttgtcgaac
    11161 agcaggtacg ccacttcacc gccaaccaca ccgacctcga ccacaaacac cgcctcatgc
    11221 tgctcaccgg ccccaatatg ggcggcaaat ccacctatat gcgccaagtc gcgctgattg
    11281 ttttattggc acacaccggc tgtttcgtgc ctgccgatgc cgccacaatc gggcccatcg
    11341 atcaaatctt cacccgcatc ggcgcatcgg acgacctcgc ctccaaccgc tccactttca
    11401 tggtcgaaat gagcgaaacc gcctacatcc tgcatcacgc caccgaacaa agccttgttt
    11461 taatggacga agtcggacgt ggtacttcca ctttcgacgg cctcgccctc gcgcacgccg
    11521 ttgccgaaca cctgctgcaa aaaaacaaat ccttcagtct gtttgctacc cactatttcg
    11581 agctgaccta cctgcccgaa gcccacgccg ccgccgtcaa tatgcacctt tccgcgctcg
    11641 aacagggaca ggacatcgtt ttcctgcacc aaatccaacc gggtcccgcc ggtaaaagct
    11701 acggcattgc cgtcgccaaa ctcgccggcc tgcctgtacg cgcattgaaa tccgcccaaa
    11761 agcatttgaa cggactggaa aaccaagccg ccgcaaaccg tccccaactg gatattttca
    11821 gtaccatgcc gtctgaaaaa ggagatgaac cgaatgtggg caactttgtg gacaaagcag
    11881 aggaaaaaca ttttgaaggt atattggcag cagccttgga aaaactcgat cccgacagcc
    11941 tgaccccgcg cgaagcattg tcagaactgt accgtctgaa agatttgtgc aaatccgtat
    12001 cttaatttcc gttggcggaa cagcatcaaa ccatatggaa aaatctgtgg ataaacatta
    12061 tctgacaaga agtttccaaa cataaaaatg ccgtccgaac agctcagacg gcatttgtcc
    12121 attcggctta aaccttatcc atatccaaac gcataaccgt aacccattca ccgttatgga
    12181 aatgtcgccc gacaaccgcc cagccgaatg attcataaaa tatttgcaca tcaggcgtat
    12241 aaagatacaa gaactttatc cccagcgaac gcgctgcgcc tatgcagtgg gcgaccagcc
    12301 tcctgccaat gccttttccg cgatattcag gtaaaacaaa gacatcaccc aaccaatatt
    12361 catactgtgg aaaactttcc atatcatgcc gcttgaccgt agccgaaccc aacagggttc
    12421 cggaatcatc cacagccgca aaagccagcg gcagttcgtc atccttcaaa cacctgccgt
    12481 aataggcatg aatcttatcc acagaagacc acggttcaaa tccgtgccac tcctcaaaca
    12541 acgcctgaac caacctgccg atatgcccgg ctttcagccg tgtaatgaaa acagtattgt
    12601 ctacaaagag ggaattcatc ggtcaattcc ccgacgcttt cgttccccct gcgccgtaaa
    12661 ccgtattcca agcatggtcc aaacgcactc cgatttgcct cagctcttca gcctgccggg
    12721 ctttttgcgc cattgctgca ggaatttccg cttccaaacg ggcgatgtct gcttgagccg
    12781 tctgcaaacg ccggcgcgca tcttccaaat ccgactgcat cccgatgatt tttccgtcca
    12841 gattgttttg tttttgcagt aaggcacgat agccggattg gatgctgagc agattgtctt
    12901 ccgcatcccc ggcccatacg cttgtagaaa aaacaaacat cagaaaatac aatatttttt
    12961 tcatgttcaa cttccgtttg aatgctgtct gaagccgcat tccgacatca gacggcatcg
    13021 cccacgcctg tggataactt aagcgcggat gcgtttcaac acttcttctt tgccgattaa
    13081 tgccaacaca gcatcgacgc tgggggtttt cgccgtaccg cagacggcaa ggcgcagggg
    13141 catgccgagt ttgcccattt taatgccttc ttcgtcgcag aagggtttga agaggtcgtg
    13201 gatggcttcg gcattccagt cttccagccc ttcgaggcgt tcggcaaagc gcagcatacg
    13261 ggcggcggct tcatcgtccc agtgtttctg cacgtccgct tcagtaggcg tttgtttgac
    13321 gtagaagtag aggcactcgt cggcaagtgt gttcaggtct tgggcgcggt ctttgaccag
    13381 tgccaacacg tcttccaatg caggtttttc agtttcatgg atatcgcgca gagcgagacg
    13441 cggtttgacg agttcggcga gtttgccgtt gggcgtgatt ttgatgtgtt cgccgttgat
    13501 ccagtagagt ttcttcaaat ccatgcggct tggagatgga gaaacgtctt tcaaatcaaa
    13561 ccattcgata aattgttcca tcgtgaagaa ctcatcgtcg ccgtgcgccc agcccaaacg
    13621 tgccagatag ttgagcatgg cttcgggcag gatgcccatc gcgccgaaat cggtaatggc
    13681 aacggtatcg ccgctgcgtt tggagatttt tttgccttgt tcgttgagaa tcatgggcag
    13741 gtgtccgtat tcgggcaggt ttgcgccgat ggcttttaag atgttgattt gtttcggggt
    13801 gttgttcaca tggtcgtcgc cccggataac gtgggtaacg cccatgtcgt agtcgtccac
    13861 cacgacgcag aagttgtagg tcggtgtgcc gtcggcgcgt gcgataatca ggtcgtcgag
    13921 ggcttcgttg gggatggaaa tttcgccttt gaccaagtcc gcccacttgg ttacgccatc
    13981 caaaggcgtt ttgaagcgga cgacgggttg cacgccggca gggatttcgg gcagggtttt
    14041 gcccgcttcc ggccgccagc ggcggtcgta agtcgccgtg ccttcttttt cggctttctc
    14101 gcgcatggct tccaactctt ctttgctgca atagcagtag taggcatcgc ctttttctaa
    14161 aagttcggcg atgacttctt tatagcggtc gaaacggcgg gtttggtaca cgacgttgcc
    14221 ggcgttgtcg taatcgagtc cgacccattt catgccgtcg aggatgatgt tgacggattc
    14281 ggcggtagaa cgcgccaagt cggtgtcttc gatgcgcaat aaaaattcgc ctttatgatg
    14341 gcgggcaaac gcccaagaaa acagggcggt gcgaacgccg ccaatgtgca ggtagccggt
    14401 tgggctgggg gcgaaacggg ttttgacggt catgatggct ccaaaatctt taaaacggct
    14461 tattttactg gttttaccgt gcttgggcat caaaaatgcc gtccgaaccc tgcctgcgga
    14521 taagtttcag acggcatttt ctaccgcttc aatcagacag ccatcccaaa aagcggctgc
    14581 cccatttttc caaacggtag agggataacg catatccctc ttgcagcata aagatttttt
    14641 tcttatttcc cgcatcaaac cgcgtggtcg gggtggcaga catataaacg cggacaccca
    14701 aatcctccgc catttccgcc gcccgcgcca aatggtaggg atcgctgaca atcaccacgc
    14761 tgtcaatacc gttggcacgc aaaaccggac ggatgttgtt caggttttca taagtgttgc
    14821 gcgaagtgtt ttcaaacagg atgttgcgcg ccggaacccc ctgtttgagc gcgtaccgcc
    14881 gcccgacctc ggcttcggtc atatagcctt ttttggttcg ccctcccgta aacacgattt
    14941 tgcctaccct gcggctctga taaagcgcga tggcgtggtt gatgcgttcg cggaaaaccg
    15001 gggaagggcg tttgtcccac gcggcggcgc ccaacaccag cgcggcatcc gcccggacat
    15061 acggcggcaa aacctgcccg cccgtccgat aaaccgccca aacggatgag gcaaacacca
    15121 gcaaaagcgg aaaaacactc aaacagaaac cgcccaacag gtaatagcgc aagccgttgc
    15181 ggctgcaaaa cagccgtttg ttcacaatat cgcttcgata ttttccagag gcctgccgac
    15241 agccgcctta ccgttcgcca aaacaatcgg acgctccaac agggcgggat gctcggcgat
    15301 ggcacgcagc agcgcgtcat tgtccaaatc ggggttgtcc aaacccaatt ccttgtacaa
    15361 atcatctttc acacgcatca tcccgcgcgc cgatgccaag cccaatttgt tgaaaatatc
    15421 cttcaattcg gacaagtcgg gcggcgtatc caaatatttg accacttcgg cagcaatgcc
    15481 gcgttcttcc aatagggaca aggcggcacg cgatttgctg caacgcggat tgtggaaaat
    15541 tttgatttca ggcatgacat ttccttgctt ctcaacaatc cccttattat cggcttacgc
    15601 agggttttac tcaatatccc gcccccaacc gtaccaaacg gtttacaata cccgaatcga
    15661 catacaaagg acaaaacgat gaaatacttg aatcttgccg caatcgccct tgccgccaca
    15721 tttgccgcac ataccgcctc ggcagacgaa cttgccggat ggaaagacgg taccgcgcaa
    15781 agcctgcaat cgctcaaagc ccccgtacgc atcgtcaacc tgtgggcgac ttggtgcggc
    15841 ccgtgccgaa aagagatgcc cgccatgtcc aaatggtaca aagcgcagaa aaaaggcagc
    15901 gtcgatatgg tcggcatcgc gctcgacaca tccgacaata tcggcaactt cctcaaacaa
    15961 actcctgttt cctacccgat ttggcgttac accggggcga acagccgaaa ctttatgaaa
    16021 acctacggaa acactgtcgg cgtactgccc tttaccgtcg tcgaagcacc gaaatgcgga
    16081 tacaggcaga ccattaccgg ggaagtgaac gaaaaaagcc tgaccgaagc cgtcaaactc
    16141 gcccattcaa aatgccgtta aacgccggat gccgtctgaa gccgcttcag acggcatttt
    16201 tcccgcccga ccttcatatc cgccaaactt atccactatc taaaagcagg cggaatcttt
    16261 ataatcggca ctgtcttacc tattgttcag acggcatatc cctgcggacg caaccgcccg
    16321 aaacgatatg ccgcccttcc ttacaggacc tcctatgatc cgtttcgaac aagtttccaa
    16381 aacctatccc ggcggttttg aagccctgaa aaacgtcagc ttccaaatca acaaaggcga
    16441 gatgattttt atcgcaggac actccggttc aggcaaatcc accgtcctca agctgatttc
    16501 gggcattacc aaaccaagca ggggcaaagt ccacttcaac aaccaagacc tcggcacact
    16561 gaccgacaac caaatcggct ttatgcgcca acacatcggc atcgtgttcc aagaccacaa
    16621 aatcctctac gaccgcaacg tcctgcaaaa cgtcatcctg ccgcttcgga ttatcggcta
    16681 tccgccgcgc aaagccgaag aacgcgcccg catcgccatc gaaaaagtcg gcctgaaagg
    16741 acgagaattg gacgatcccg taaccctctc cggcggtgaa caacaacgcc tgtgcatcgc
    16801 ccgcgccgtc gttcaccagc ccggcctgct gattgccgac gaaccctccg ccaacctcga
    16861 ccgcgcctac gcgctcgaca tcatggaatt gttcaaaacc ttccacgaag cgggaacaac
    16921 cgtcatcgtt gccgcccatg acgaaaccct gatggcggac tacggacacc gcatcctgcg
    16981 cctctcgaaa ggacgactcg catgagcatc atccactacg cctcgctgca catcgaatcc
    17041 gcccgcaccg cgctcaaaga gcttctgcgc caacccttcg gcacgctgct caccctcctg
    17101 atgctcgccg tcgcgatgac cctgccgctg tttatgtatt tgggcattca aagcgggcaa
    17161 agcgtgttgg gcaaactcaa cgagtcgccg caaatcaccg tctatatgga aaccgccgcc
    17221 gcacaaagcg acagcgatac cgtccgcagc ctgctggcgc gcgacaaacg gatcgccaac
    17281 gtccgcttca tcggcaagga agacggcttg gcggaattac agtccaacct cgaccaaaat
    17341 ttggtttcga tgctcgacgg caacccgctg cccgacgcat ttatcgttac ccccgacccc
    17401 gcgacaacgc ccgcgcaaat gcaggcaatc taccgggaca ttaccaaact gcctatggtc
    17461 gaatccgcgt ctatggatac cgaatgggtg caaacgctgt accaaatcaa cgaatttatc
    17521 cgtaaaattt tatggtttct ttccctgacg ctgggtatgg cgttcgtcct tgtcgcacac
    17581 aacaccatcc gcctgcaaat cctcagccgc aaagaagaaa tcgaaatcac caaactcttg
    17641 ggcgcgcccg catcgttcat ccgccgcccc ttcctttatc aggcaatgtg gcagagcatc
    17701 ctttccgccg ccgtcagcct ggggctttgc ggttggctgc tttccacagt gcgccccttg
    17761 gtcgatgcca tcttcaaacc ctacgggctc aatatcggct ggcgtttctt ttatcctgaa
    17821 gaaatcgcgc tggtgttcgg cttcgtcatc gcgttgggcg tattcggcgc gtggcttgcc
    17881 accacccagc acctgctcgg cttcaaagcc aaaaaataaa acaccgtcaa aaatgccgtc
    17941 cgaacccgtt ttcagacggc atttcaattt gccagtataa tggcgcattt ttccaacaag
    18001 gaacctacca tgctgacctc ggaacaagta aaagccatga ttgaaggcgt ggcaaaatgc
    18061 gaacatatcg aagtagaagg cgacggacac cattttttcg ccgtcatcgt ttcatcagaa
    18121 tttgaaggca aggcacgcct cgcgcgccac cgcctgatta aagacggact caaagcccaa
    18181 ctggaaagta acgaactgca cgcactttcc atttcggttg ccgccactcc ggcggaatgg
    18241 gcagccaaag cacaataatc gccacacaaa aatgccgtca gaaacagcac aacgtttcag
    18301 acggcatttt ttatatcaaa ccgcttacgc gccgcgtttt tccaaagcgg ctacggcagg
    18361 caactcttta ccttccaaga actcaagaaa cgcgcctccg ccggtagaaa tatagccgat
    18421 ttggtcggta acgccgaatt tagcaatcgc cgccagcgtg tcgccaccgc ctgcaatcga
    18481 gaatgctttg ctttgggcaa tggcttcggc aagagctttc gtgccgcctg cgaactggtc
    18541 aaactcgaac acgccgaccg gtccgttcca aacgaccgtg tcggcggctt taagcaaatc
    18601 ggcaagcgcg gcggcagatt tcgggccgat gtccaaaatc atctcgtctt cggcaacgtc
    18661 ggcaatgtct tttaccaccg cttcggcatc ggcggcaaag gctttggcaa cgacgacatc
    18721 ggtcggcagc ggcacagaac ctcctttggc cgccattttc gccataattt ttttggattc
    18781 ttccaccaaa tcgtgttccg ccaaagattt gccgatggct ttaccttccg ccaacaggaa
    18841 ggtgttggcg ataccgccgc cgacgatgag ttggtcgact ttgtccgcca gcgattcgag
    18901 gatggtcagc ttggtggaca ctttgctgcc ggcaacgatg gcaaccatcg ggcgcgcggg
    18961 ctgtttcaag gctttgccca aagcgtcgag ttcgcccgcc atcaatacgc cggcgcaggc
    19021 aacgggagcg gcttgggcga cggcttcggt cgaggcttgg gcgcggtgcg cggtgccgaa
    19081 cgcgtcattg acgaacacgt cgcacaaaga agcgtaggct ttgcccagtt ccaaatcgtt
    19141 tttcttctcg cctttgttga tacgcacgtt ttgcagcatc acgacatcgc ccgcgttcaa
    19201 agccggtttg ttttcgcgcc agtcgttcaa tactttcacg tttttgccca acaggccgcc
    19261 caaatgcgcg gcaacgggcg caacgtcgtc ttcggggtga aactcgcctt cggtcgggcg
    19321 gcccaagtgg gtcatcacga tgacggacgc gccgttgtcc acgcagtatt taacggacgc
    19381 aatcgaggcg cggatacggg tgtcgtcgct gattttgccg tctttgaacg gcacgttcat
    19441 atcggcgcgg atgaggacgg ttttgccctg cacgttttgt tcggtcagtt ttaaaaatgc
    19501 cataatcagt ccttttcaat cagtgtttgc gatacagaaa caat
//
LOCUS       DAWFBO010000024        18191 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFBO010000024 DAWFBO010000000
VERSION     DAWFBO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.024"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(135..470)
                     /locus_tag="WGO87_000592"
     CDS             complement(135..470)
                     /locus_tag="WGO87_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0391985.1"
                     /translation="MFKDWKEHTALVKKSFGELGKSHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            599..1504
                     /gene="mtrA"
                     /locus_tag="WGO87_000593"
     CDS             599..1504
                     /gene="mtrA"
                     /locus_tag="WGO87_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0391986.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1566..2054)
                     /locus_tag="WGO87_000594"
     CDS             complement(1566..2054)
                     /locus_tag="WGO87_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0391987.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2201..3103
                     /locus_tag="WGO87_000595"
     CDS             2201..3103
                     /locus_tag="WGO87_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0391988.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLAEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3144..4274)
                     /locus_tag="WGO87_000596"
     CDS             complement(3144..4274)
                     /locus_tag="WGO87_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0391989.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4479..7103
                     /gene="alaS"
                     /locus_tag="WGO87_000597"
     CDS             4479..7103
                     /gene="alaS"
                     /locus_tag="WGO87_000597"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0391990.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALVKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSEQA
                     IVLLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLASVESWLCQKLS"
     gene            7165..7896
                     /locus_tag="WGO87_000598"
     CDS             7165..7896
                     /locus_tag="WGO87_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0391991.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8205..11018
                     /locus_tag="WGO87_000599"
     CDS             8205..11018
                     /locus_tag="WGO87_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0391992.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGAENQWQFPLEELPEYITRGVFDFMFGIEAKVRQI
                     QEEANIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFRRNYLFQDF
                     LENCKEADTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWRNISPEIFGTLFQSVMDAQERREAGAHYTEAANIDKVINGLFLE
                     NLRAEFEAVKALKRDKAKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDII
                     AEALKDKAGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDG
                     EVACHTLPLEDAAEIIHANSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHI
                     KGYGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQGIEIHF
                     AHRTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKNLYDYPDIKGEPVKHTAANINPYL
                     IDAPDLIITKRRSQISGEIEMLYGNKPTDGGNLILSTAEKDALIAAEPLAEQYTRPFV
                     GAEEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKAMREASSKAATQKKAA
                     TPWLFDEIRQPSEGNYLIIPRVSSESRRFVPIDYLSHQIIISDSSFMLPNATLYHFGI
                     LSSTMHNAFMRTVAGRLKSDYRYSNTVVYNNFPFPESCRMPSENDCSDPLRAAVEAAA
                     QAVLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGK
                     NTDDEAERTAFLFELYRKAAAIA"
     gene            complement(11820..12503)
                     /locus_tag="WGO87_000600"
     CDS             complement(11820..12503)
                     /locus_tag="WGO87_000600"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0391993.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            12800..15103
                     /gene="parC"
                     /locus_tag="WGO87_000601"
     CDS             12800..15103
                     /gene="parC"
                     /locus_tag="WGO87_000601"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0391994.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            15124..16641
                     /locus_tag="WGO87_000602"
     CDS             15124..16641
                     /locus_tag="WGO87_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ0391995.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQALKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
                     TASATFILVAASYLSAIFTSLSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAV
                     VVINTEGLAVLFNRKAKDLFPALEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTA
                     RIRAVPMNKEPDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            16693..17160
                     /locus_tag="WGO87_000603"
     CDS             16693..17160
                     /locus_tag="WGO87_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0391996.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            17264..18016
                     /locus_tag="WGO87_000604"
     CDS             17264..18016
                     /locus_tag="WGO87_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0391997.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         4559 a         5256 c         4392 g         3984 t
ORIGIN      
        1 ttcacgctta ccccttgttt ctcaaatgcc gtctgaaaaa aacggctgta tatgttgtct
       61 gcaaaatatc gggaagcata acagacaaaa tgccgtctga aatattttca gacggcattt
      121 caatccgatt cggattattt ttgcgtttca accgcttcca atgcacgcag ggcgtaagtg
      181 taagcggcac cggcattcag ggcgatggcg gttgccaatg cgccggcgat ttcgctgtcg
      241 gtcgcaccgg ctttggcggc ggctgcggcg tgaacgctga tacagctttc gcaacgggtg
      301 gtaatggcaa cggcgatggc aatcagttcg cgcgttttgg catccaatgc ttcggcggcg
      361 gcggcctgtt ccaatgcgcc gtatgcctgc agcattttag gatgggattt acccagctcg
      421 ccgaacgatt ttttaaccaa tgcggtatgt tctttccaat ctttaaacat tttcttttcc
      481 tttctcttgc gtttaaccct gatacgcgct tgcgtatctg ttttcgatgt gcgtattatt
      541 gcaattattc agttgtgttt ctcgtttaat catctcattt catggttcaa aaagatttat
      601 ggacattctg gacaaactgg tcgatttcgc ccaattgacg ggcagtgtgg atgtgcagtg
      661 ccttttgggc ggacaatggt cggtacggca tgaaaccttg caacgcgaag gactggtaca
      721 cattgttaca tcgggcagcg gctatctctg catcgacggc gaaacttccc cgcgtccggt
      781 cagtacaggg gatattgtat ttttcccgcg cggcttgggt catgtgttga gccacgacgg
      841 aaaatgcgga gaaagtttac aaccggatat gcggcagcac ggtgcgttta cggtcaagca
      901 gtgcggcaac ggacaggata tgagcctgtt ttgcgcccgt ttccgctacg acacccacgc
      961 cgatttgatg aacgggctgc ctgaaaccgt ttttctgaac attgcccatc cgagtttaca
     1021 gtatgtggtt tcaatgctgc aactggaaag caaaaaacct ttgacgggga cggtttccat
     1081 ggtcaacgca ttgtcgtccg tcctgctggt gcttatcctg cgcgcctatc tcgaacagga
     1141 taaggatgtc gaactctcgg gcgtattgaa aggttggcag gacaaacgtt tgggacattt
     1201 aatccaaaag gtgatagaca aaccggaaga cgaatggaat gtcgacaaaa tggtggcggc
     1261 tgccaatatg tcgcgcgcgc aactgatgcg ccgtttcaaa agccgggtcg gactcagccc
     1321 gcacgccttt gtgaaccata tccgcctgca aaaaggcgcg ttgctgctga aaaaaaaccc
     1381 agattcggtt ttgtcggtcg cactgtcggt aggctttcaa tcggaaacgc acttcggcaa
     1441 ggcgttcaaa cggcaatatc acgtttcgcc gggtcaatac cggaaagaag gcgggcaaaa
     1501 ataaatcggg gcttcaaacg caaatgccgt ctgaaaaggc tttcatacgg catttgcgta
     1561 ccgcgtcatt tcaagggctg catcttcatc acttccatca aaaagttggt aaatgcgggg
     1621 ttgttgggtt tgacatccat atttttccaa cgctgctgcc agccgcgcaa ggcattctgg
     1681 atatacagct tggactgttc cgtattgatt gcgccccgct ggctgtctat cgccgaacgc
     1741 aggtagattt catacatact gtcatcgacg gcattgcgtc cgaccaggcg ttttctgaag
     1801 ttgttcagat attgcgccgc ctgaaccttg gtcattttac cgatacccac ctgatagccc
     1861 aagcgcgtcg cttcatcgct gattttggca acatccgtcc aatgcgaaga ggcaaggcgg
     1921 aaaccttttg caggtgcttc cgttttgacg gtattgatag gattcacggg gatttccgtc
     1981 aatgtgggca cataaataga ctggcagccg gaaagaactg ccacaatgga aagagggata
     2041 aggtattttt tcatgccccc attataatca agtttgcctt gagaaaacaa attgttcggc
     2101 aagaaaaata aaatttcggc atcagaagca ggcaaaaaca cattccacaa gccttgccgc
     2161 aaggtttaca atccgaccgt ccttatcgca acgaccgttt atggataccg caaaaaaaga
     2221 cattttagga tcgggctgga tgctggtggc ggcggcctgc tttaccatta tgaacgtatt
     2281 gattaaagag gcatcggcaa aatttgccct cggcagcggc gaattggtct tttggcgcat
     2341 gctgttttca accgttgcgc tcggggctgc cgccgtattg cgtcgggaca ccttccgcac
     2401 gccccattgg aaaaaccact taaaccgcag tatggtcggg acgggggcga tgctgctgct
     2461 gttttacgcg gtaacgcatc tgcctttggc caccggcgtt accctgagtt acacctcgtc
     2521 gatttttttg gcggtatttt ccttcctgat tttgaaagaa cggatttccg tttacacgca
     2581 ggcggtgctg ctccttggtt ttgccggcgt ggtattgctg cttaatccct cgttccgcag
     2641 cggtcaggaa acggcggcac tcgccgggct ggcgggcggc gcgatgtccg gctgggcgta
     2701 tttgaaagtg cgcgaactgt ctttggcggg cgaacccggc tggcgcgtcg tgttttacct
     2761 ttccgtgaca ggtgtggcga tgtcgtcggt ttgggcgacg ctgaccggct ggcacaccct
     2821 gtcctttcca tcggcagttt atctgtcgtg catcggcgtg tccgcgctga ttgcccaact
     2881 gtcgatgacg cgcgcctaca aagtcggcga caaattcacg gttgcctcgc tttcctatat
     2941 gaccgtcgtt ttttccgctc tgtctgccgc attttttctg gccgaagagc ttttctggca
     3001 ggaaatactc ggtatgtgca tcatcatcct cagcggcatt ttgagcagca tccgccccac
     3061 tgccttcaaa cagcggctgc aatccctgtt ccgccaaaga taaaaaatgc cgtccgaaca
     3121 tccttcagac ggcatatcgg gctttatttc cccgccttca catcctgcca ctggcgcacc
     3181 ataaacttca atgccgccgg ctggataggc accatgataa agctgttttt caaatcctcc
     3241 tcggttggga aaatcgtatt gtcgttttta aattcgtctt ccatcagctc acgcgccggc
     3301 ttgctcgaag gcgcataagt aacgaaattg ccgtttttcg ccgacacttc cgggtcgagg
     3361 aagtcgttga tgtatttgtg cgcgttggcg acgtttttcg catctttcgg aatcacgaaa
     3421 gaatccaccc aaatccccac gccctctttg ggcatcatca cgcggatttt ttccttgccg
     3481 cccgcttctt cggcacggcg tttggcgatg ttcaaatcgc cgccgaaacc gattgttacg
     3541 caggtatcgc cgcgcgccaa atcatcgata aagccggacg aagtaaagcg tttgatattg
     3601 gggcggtttt tcttgagcag ggcggttgcc tccctgatgt cttccgtatt gctgctgttc
     3661 gggtttttac ccaaatagtt caacaccata ggatagattt ccgccgcgct gtctaaataa
     3721 ctgataccgc attgtttgag tttggacgtg tattcggggt cgaacaccaa atcccactgg
     3781 ttgtccggca gcttgtccgt acccaaagcc tttttcacgc gttcggtatt gatggcgaag
     3841 gtatttgtcc cccaataaaa cggcacggcg tattcgtggc cgggatcgac cccgtccatc
     3901 agcctcatca tttcgggatt gaggtgttta taattgggaa tcagcgattt atcgattttc
     3961 tgatacgcac ctgccttaat ctgcctgccc acaaacgcat tggacggcgc gacaatgtcg
     4021 taaccggatt ttccggtcag caccttgctt tccagcgttt catcgctgtc gtacacatca
     4081 taagtaacct tgatgccgtt tttcttttca aaatcggcaa cggtttccgg atcgacgtat
     4141 tccgaccagt tgtaaatttt caatacgttt cggttttccg ccggcgccgg tttttcggca
     4201 ggcggtttgt ccgaaccgcc gcacgctgca agcagcaaag cagtcaggac ggcgagtggc
     4261 agatgtttgg ccattatcat tccttgcata tcgggttgga gaaagcggtc attatagccg
     4321 atattggcaa cagggcttca gacggcattc aaaatcccgc cacacccttc cgaaaaccgc
     4381 cgcttccata gctagaaaca gggatttgcg gtaagatacc gccgttcgtt ttccctgctt
     4441 ttaccatgac aagacatttg agagacattg aaaaaattat gaaaacctcc gaactgcgcc
     4501 aaaaattcct aaaatttttt gaaaccaaag gccacaccgt cgtccgctct tccagcctcg
     4561 tgccgcacga cgacccgacc ctgttgttta ccaacgcagg catgaaccag tttaaagacg
     4621 tatttttagg tttcgacaaa cgcccgtaca gccgcgccac caccgcgcaa aaatgcgtac
     4681 gcgcaggcgg caagcacaac gacctggaaa acgtcggcta caccgcccgc caccacacct
     4741 tctttgaaat gatgggcaac ttctccttcg gcgactactt caaacgcgac gccatccact
     4801 ttgcttggga atttctgact tcccccgaat ggctcaacat ccctaaagac aaactgttgg
     4861 cgaccgttta cgcggaagac gacgaagcct acaacatctg gttgaacgaa atcggtatgc
     4921 cgtccgagcg catcgtccgc atcggcgaca acaaaggcgc gaaatacgcg tccgacaact
     4981 tctggcaaat gggcgacacc ggcccttgcg gcccctgctc cgaaattttc tacgaccacg
     5041 gcgaagaaat ttggggcggc attcccggca gccccgaaga agacggcgac cgctggatcg
     5101 aaatttggaa ctgcgtattc atgcagttca accgcgacga acaaggcaat atgaatccgc
     5161 tgcccaagcc gtccgtcgat accggcatgg gcttggaacg catggccgcc gtcatgcagc
     5221 atgttcacag caactacgaa atcgacttgt tccaagacct gctcaaagcc gttgcccgcg
     5281 aaaccggcgc gccgttcagt atggaagaac ccagcctgaa agtcatcgcc gaccacatcc
     5341 gctcctgctc gttcctgatt gcagacggcg tcttgccttc caacgaaggc cgcggctacg
     5401 tattgcgccg cattatccgc cgcgccgtgc gccacggtta caaactgggt caaagcaaac
     5461 cgttcttcca caaactcgtt gccgatttgg tcaaagagat gggcggtgcc taccctgaat
     5521 tgaaagaaaa acaagcccaa atcgaagaag cattgaaaaa cgaagaaagc cgttttgccc
     5581 aaacgctgga aaccggtatg gctttgttgg aaaacgcgct ggtcaaaggc ggcaaaacac
     5641 tcggcggcga aatcatcttc aaactctacg atacctacgg tttcccatac gacttgactg
     5701 ccgacatctg ccgcgaacgc aatatcgaac cggacgaagc aggcttcgag cgcgaaatgg
     5761 aagcccaacg cgcacgcgca cgcgccgccc aaagcttcaa agccaacgcc caactgcctt
     5821 atgacggtca agacaccgag tttaaaggtt atagcgaacg ccaaaccgaa tccaaagtcc
     5881 tcgccctcta caaagacggc gagcaagtca acgaattgaa cgaaggcgac agcggcgcag
     5941 tcgtcatcga ctttaccccg ttctatgcag aatccggcgg ccaagtcggc gatgtcggct
     6001 atatcttctc aggcgaaaac cgctttgaag tacgcgatac ccaaaaaatc aaagcggccg
     6061 tattcggtca attcggcgta caaacttcag gccgtctgaa agtcggcgac agcgttaccg
     6121 ccaaagtgga tgacgaaatc cgcaatgcca atatgcgcaa ccacagcgcg acccacctga
     6181 tgcacaaagc cctgcgcgac gtattgggcg gacacgtcga acaaaaaggc tctttggtta
     6241 ccgccgaatc cacccgtttc gacatttccc atcctcaagc ggtcactgcc gaagaaatcg
     6301 ccgaagtcga acgccgcgtc aacgaagccg ttttggcgaa cgttgccgtc aatgcagcca
     6361 ttatgagcat ggaagacgcg caaaaaaccg gcgcgatgat gctcttcggc gaaaaatacg
     6421 gcgaagaagt gcgcgtactg caaatgggcg gtttctctac cgaattgtgc ggcggcacac
     6481 acgtttcacg caccggcgac atcggcctct tcaaaatcat cagcgaaggc ggtattgccg
     6541 caggcgtgcg ccgtatcgaa gccatcaccg gcctgaacgc actcaaatgg gcgcaagagc
     6601 aagagcgttt ggtgaaagac attattgccg aaaccaaagc ccaaaccgaa aaagacgtac
     6661 tggcaaaaat ccaagcaggc gcggcacacg ccaaagcatt ggaaaaagaa ttggcacgcg
     6721 ccaaagccga actcgccgtc cacgcaggcg ccaaactctt ggacgatgca aaagacttgg
     6781 gcgcagccaa actcgttgcc gcccaaatcg aagccgacgc agccgccctg cgcgaaatcg
     6841 taaccgacct gaccggcaaa tcagaacaag ccatcgtatt gctcgccgca gtcaacgagg
     6901 gcaaagtttc cctgtgcgca ggcgtatcca aagccctgac cggcaaagtg aaagcaggcg
     6961 atctggtcaa atttgcagcc gaacaagtcg gcggcaaagg aggcggccgt cccgacttgg
     7021 cgcaggccgg cggcacggat gccggcaaat tgcctgaaat gctggcaagc gtggaaagct
     7081 ggctttgtca gaaattaagt taaatttaaa tcttaataag ccatttgagt cttgttcaaa
     7141 tggcttatat tttggattgt tagaatgaat actaaaacag agttacaaaa acttttagaa
     7201 gaggatatat caactttaac agaaacacta atttgtgcag atgctcttcc tccaagatat
     7261 gtacgtagca ttgcaactcc tattgtccgt cgatggttaa ttgacaaaca attaaatatt
     7321 ctcgcaaaag aaataggttt aactatagaa ttgcccatcc ttgatacaag tttggtattt
     7381 gaaaaattgt caaccttaga gaataaagtt aatttttata tggctggtgg agtttattta
     7441 ggcggagaat ttattagctc tatatatcat tcatcacaag aattttctgg tgaaccaata
     7501 atctatgctg aaccaaatat aattttatgt cctgctgaaa aatttttaac tttaaaacga
     7561 gttttccata atggaaatat ttttaatatg aatcagataa ttactttttt atccaataaa
     7621 caaggaggag tacattttga taaaaattac gataaatata aaacttggca agttgctata
     7681 gaaaaagcag caaatttttt aaaactcggt aatccatata atgaagataa attatctctt
     7741 agtgaagaac atgatactat tttagtagtt ttaccattag aaaaaggata tgaatggaat
     7801 tgtcttgaga tagaagtact atccgctgct caatctctag caaatatcta ttgcaacaaa
     7861 gttcgcctca tagatggtca tgtttggaag gaataaaaga aaaagcatag ttggtaattt
     7921 ttagttacgt agggtgggct tcagcccacc gttttttcat ttttgattgt tgattggtgg
     7981 gctaaagccc accctacggt tactaaaatc ccgtcattac ctcgcaggcg ggaatccggt
     8041 ttgttcggtt tcggtttttt agggacagtg ccgcaacatt aaatttccag attcccactt
     8101 tcgcacaaat aacagcgcaa atagggcaaa gataaggata tggggggttt tttctttgaa
     8161 tttactatac aattcgcccg tccccgttat agaaacccaa gccgatgaaa accctgctcc
     8221 aactccaaac cgccgcacaa aacttcgccg cctactacaa agaccaaacc gacgagcgcc
     8281 gcgagaaaga caccttctgg aacgaatttt tcgccatttt cggcatcgac cgcaaaaacg
     8341 tcgcccactt cgaatacccc gtcaaagacc ctgccgacaa cacccaattc gtcgatatat
     8401 tttgggaagg catcttcctt gccgaacaca aatccgccaa caaaaacctg accaaggcca
     8461 aagagcaggc ggagcgttat ttgcaggaaa tcgggcgcac caagccctcc gcgctgcccg
     8521 aatattacgc cgtcagcgat tttgcccatt tccaccttta ccgccgcgta cctgaagaag
     8581 gcgcggaaaa ccaatggcag ttcccattgg aagaattgcc cgaatacatc acgcgaggcg
     8641 ttttcgactt catgttcggc atcgaagcca aagtccgcca aattcaagaa gaagccaaca
     8701 ttcaagcggc ggcggccatc ggcaggctgc acgacgcgct caaagaagaa ggcatttacg
     8761 aagaacacga gctgcgcctc ttcatcacgc gcctgctttt cctctttttt gccgacgaca
     8821 gcgccgtttt ccggcgcaac taccttttcc aagacttttt agaaaactgc aaagaagccg
     8881 acacgctcgg cgacaagctc aaccaactct ttgaatttct caacacaccc gaccaaaagc
     8941 gcagcaagac ccaaagcgaa aaatttaaag gtttcgaata cgtcaacggc ggcctgttta
     9001 aagaacgcct gcgcaccttc gacttcaccg ccaagcagca ccgcgcctta atcgactgcg
     9061 gcaatttcga ctggcgcaac atcagccccg aaatcttcgg cacgctcttc caatccgtca
     9121 tggacgcgca agagcggcgc gaagcaggcg ctcactacac cgaagccgcc aacatcgaca
     9181 aagtcatcaa cggccttttt ttagaaaacc tgcgcgccga atttgaagcc gtcaaagccc
     9241 tcaaacgcga caaagccaaa aaactcgccg ccttctacca aaaaatccaa aacctgcaat
     9301 tcctcgaccc tgcctgcggc tgcggcaact tcctgatcgt tgcctacgac cgcatccgcg
     9361 cccttgaaga cgacatcatc gccgaagcgc tcaaagacaa agcaggcggc ctgttcgaca
     9421 gcccgtccgt ccaatgccgt ctgaaacagt ttcacggcat cgaaatagac gaatttgccg
     9481 tcctcatcgc ccgcaccgcc atgtggctca aaaaccacca atgcaacatc cgcacgcaaa
     9541 tccgcttcga cggcgaagtc gcctgccata cgctgccgct cgaagacgcc gccgaaatca
     9601 tccacgccaa cagtctccgc acaccttggc aggcggcgga ctacatcttc ggcaatccgc
     9661 cctttatcgg ctcgacctac caaaccaaag agcagaaaaa cgacctcgaa agcatctgcg
     9721 gccatatcaa aggctacggc ctgttggatt acgtctgcaa ctggtatgtc aaagccgccg
     9781 gcatcatggc gcagcatccc caagttcaga cggcatttgt ttccaccaat tccatctgcc
     9841 aaggccagca ggtcgaaatc ctttggggca gccttttgaa gcaaggcatc gaaatccact
     9901 ttgcccaccg caccttccaa tggacgagcc aagccgcagg caaagccgcc gtccactgca
     9961 tcatcgttgg cttccgccaa aagccgcaaa tgccgtctga aaaaaacctc tacgactatc
    10021 ccgacatcaa aggcgaaccc gtaaaacaca ccgcggccaa catcaatccg tatctgattg
    10081 acgcgcccga tttgattatt accaagcgca gaagccaaat cagcggggaa attgaaatgc
    10141 tttatggtaa taagccaacc gacggcggca accttatcct ctcaaccgcc gaaaaagacg
    10201 ccctgattgc cgccgaaccc ttggcggaac aatacacccg cccctttgtc ggcgcagagg
    10261 aatttatcaa cggcaaaacc cgttggtgcc tgtggtttca cggcgtatcc gatgtcaaac
    10321 gcaaccacga cctgaaacaa atgccccaag tccaagctcg tattcaggcg gtcaaagcca
    10381 tgcgcgaagc cagcagcaaa gccgccacgc aaaaaaaagc agcgaccccg tggctcttcg
    10441 acgaaatccg tcaaccttca gagggcaatt atctaattat tccacgcgtc tcatccgaaa
    10501 gccgccgttt cgtccccatc gattatctgt cgcatcaaat catcatcagc gattcatcgt
    10561 ttatgctgcc aaacgccacc ctctaccact tcggcatcct cagctccacc atgcacaacg
    10621 cctttatgcg tactgttgcc ggccgtctga aaagcgatta tcgctattcc aataccgtcg
    10681 tgtacaacaa cttccccttc cccgaaagct gccggatgcc gtctgaaaac gactgctccg
    10741 acccgctccg cgccgccgtc gaagccgccg cccaagccgt cctcgacgcg cgcggacaat
    10801 accgccgaga agcgcgggaa gccggtttgc ccgagccgac cctcgccgaa ctctatgcgc
    10861 ccgacgcagg ctataccgcc ctagacaaag cccacgccgc cctcgacaag gcagtcgata
    10921 aagcctacgg ctacaaaaca ggcaaaaaca ccgacgacga agccgaacgc accgccttcc
    10981 tgttcgagct gtaccgcaag gcggcggcaa ttgcgtagta gttgcgtagt agttgcgtag
    11041 gatagttgcg tagggtgggc ttcagcccac caactcccac caactcccac catcttcacc
    11101 ctaaaacccc gtcatctccg caaaagcaat tgcgtagggt gggcttcagc ccaccgtttt
    11161 tcttcatttt cggttgttga ttggcaggct gaagcccacc ctgcggttac aaaaatcccg
    11221 tcattcccgc gcaggcggga atccagacct ttcggggcgg aaacttatcg gataaaacga
    11281 tttcccctgc atcctggatt cccactttca tgggaatgac gggatatagg tttccctacg
    11341 gacgtgttca gattcccgcc ttcgcgggaa tgacggcgga gaagtttctg tctttccgat
    11401 aacttaccgt aacttaaaat cccgtcatta ccgcaaaagc agttgctacg gttacaaaaa
    11461 tcccgtcatc cccacgaaag cgaaaatcct gcctgtcgga tttcggtttt ttcgggcgtt
    11521 tcgggaactt ataaatcgtc attcccgcgc aggcgggaat ccagaacgcg ggacggcggc
    11581 aatattcaaa ggttgtctga aaattcagag gttctagatt cccgcctgcg cgggaatgac
    11641 ggcggagcgg tttctgcttt tccgataaat gaccgcaacc taaaacccat ccttcccacg
    11701 aaaacagaaa aacaaaaacc taaaatcccg tcgttctcgc gaaagcagtt gctacgttta
    11761 ccgtcaaaaa tgccgtctga acgctcaagc cttcagacgg cattttttat ttcacaccct
    11821 tacaggtaga atttttcgat gactttcaaa ttgtcgtcca atttgtacac caacggctgg
    11881 ccagtcggga tttccaagcc catgatgtct ttgtcggaaa tgccctcgat gtgttttgcc
    11941 agcgcgcgca gggagttgcc gtgcgccgcc accaagacgc gtttgccgct caaaatcgcg
    12001 ggggcgattt ggtcttccca aaacggtaat acgcgctcca gcgttacttt caggttttcg
    12061 ccgtcgggta cgacatcggc aggcagatgg gcatagcggc ggtctttgtg tgcggaaaac
    12121 tcatcgtctt tgtccaaaag cggcggcagg gtgtcgtagc tgcgccgcca gatgcggact
    12181 tgctcgtcgc cgtattgttc ggcggtttgt tttttgtcca aaccttgcag ttggccgtag
    12241 tggcgttcgt tcagccgcca tgttttgatt tgcggtacga acagttggtc ggattcttcc
    12301 aaaacgatgt tgcaggtctt aatcgcgcgg gtcaggacgg atgtgaaggc gatgtcgaac
    12361 tcatagccgt tttctttcag ttttttaccg gcggcggcag cctcggcaag cccctgctcg
    12421 ctcagcttca cgtcgcgcca gcctgtaaac aggtttttcg cgttccattc gctttgtccg
    12481 tggcggataa ataccagttc catatcgtct ccaatgtgtg aaagtgggaa agccttattt
    12541 ataacatatt ttcacatttc ccgtatttga ttcagattca gacacgcgcc cactatggtt
    12601 tgccgttttg atttacaata atgccctttg ctttacattc cgcatacaca atgtttcaac
    12661 acacaggacg acacataaag caccgcccta tgtgttgccc tgatttggaa ggggttgccc
    12721 cctcccaaat aaagtctgat tctaccgccc cgaaggacag atgtccaagt ggcgggggtt
    12781 caaccgaaaa ggaaatacga taaatatgca accgcacgct ccacataccg attccaatac
    12841 gctgatgctc ggccgatacg ccgaacgcgc ctatctcgaa tacgccatga gcgtggtcaa
    12901 aggccgcgcg ctgcctgaag tttcagacgg ccaaaagccc gttcagcggc gcattttgtt
    12961 tgcgatgcgc gatatgggtt tgacggcggg ggcgaagccg gtgaaatcgg cacgcgtggt
    13021 cggtgagatt ttgggtaaat accatccgca cggcgacagt tccgcctatg aggcgatggt
    13081 gcggatggcg caggatttta ccttgcgcta tcccttaatc gacggcatcg gcaacttcgg
    13141 ttcgcgcgac ggcgacggtg cggcggcgat gcgttacacc gaagcgcggc tgacaccgat
    13201 tgcggaattg ctgttgtccg aaatcaatca ggggacggtg gatttcgtgc cgaactacga
    13261 cggcgcgttt gacgaaccgc tgcacctgcc cgcccgcctg cctatggtct tgctcaacgg
    13321 tgcgtcaggc attgcggtgg gtatggcgac cgaaatccca tcacacaatt tgaatgaagt
    13381 cacacaggcg gcgattgcac tgttgaagaa gccgacgctg gaaaccgccg acctgatgca
    13441 atacatccct gctcccgatt ttgccggagg cggtcaaatc atcacgccgg cggacgaatt
    13501 gcgccgtatt tacgaaaccg gcaagggcag cgtgcgcgtg cgtgcgcgtt acgaaatcga
    13561 gaagctggcg cgcggacaat ggcgcgtcat cgtaaccgag ctgccgccga acgccaattc
    13621 cgccaaaatc cttgctgaaa tcgaagaaca gaccaacccg aaaccgaaag caggcaaaaa
    13681 gcagctcaac caagaccagc tcaatactaa aaagctgatg ctggatttaa tcgaccgcgt
    13741 gcgcgacgag tcggacggcg aacatcccgt gcgccttgta tttgaaccaa aatccagccg
    13801 catcgatacc gataccttca tcaacacgct gatggcgcaa acttcgctgg aaggcaatgt
    13861 gtccatgaac ttggtgatga tggggctgga caaccgcccc gcgcagaaaa acctgaaaac
    13921 gattttgcag gaatggttgg atttccgcac cgttaccgta acacgccgtc tgaaattccg
    13981 tttgaaccaa gtggaaaaac ggctgcacat cctcgaaggc cgtctgaaag tctttctgca
    14041 catcgacgaa gtgattaaag tcatccgcga atcagatgac ccgaaagccg atttgatggc
    14101 ggcgttcggg ctgaccgaaa tccaagccga agacattttg gaaatccgcc tgcgccagtt
    14161 ggcacgtttg gaaggtttca aactcgaaaa agaattgaac gaattgcgcg aagaacaagg
    14221 ccgtctgaac atccttttgg gcgatgaaaa cgaaaagcgc aagctgatta tcaaagagat
    14281 gcaggcggat atgaaacaat acggcgatgc acgccgcacg ctggtggaag aggccggacg
    14341 cgccgtgctg acacaaacca ccgccgacga acccatcaca ctgattctgt cggaaaaagg
    14401 ctggatacgc agccgtgccg gacacaatct cgatttgagc caaaccgcgt tcaaagaagg
    14461 cgaccgcctc aaacaaaccc ttgaaggcag aacggtttta cccgtcgtca tcctcgattc
    14521 attgggcaga acctatacac tcgatgccgc cgaaattccc ggcggacgcg gcgacggcgt
    14581 gccggtttca tccttgatcg agttgcaaaa cggcgcgaaa cccgttgcga tgttgacagg
    14641 attgccggaa caacattatt tattatcaag cagcggcggc tacggcttca tcgccaagtt
    14701 ggctgatatg gtcggacgcg taaaagcggg caaagtggtg atgaccacag acagtggcga
    14761 aaccgtcctg ccgccgattg ccgtctatgc ctcctcgttc atcaaccccg actgcaaaat
    14821 cattgccgcc accagtcaaa accgcgccct cgccttcccc atcggcgaat tgaaaattat
    14881 ggcgaaaggc aaagggctgc aaatcatcgg attaaacgcc ggcgaatcgc tgacgcatac
    14941 cgccgtttct tccgagccgg aaatcctgat tgaaagcgaa ggcaggcgcg gcgcggcgca
    15001 caaagaccgc atccccgtct ccctgcttga ggcaaaacgc ggcaaaaaag gcaaactatt
    15061 acccatatcg ggcagcctga aacagctttc ttcccccaaa taaacccgat tccacacata
    15121 attatggtga tttccaaccc ccgcgaactt gaaaaactca aagaccggat tcccaatctg
    15181 atcaacatca tccgcgtcgc catcgttttt ccgctgatga ttatgcacat cctcgggctg
    15241 gaaaccggca gccgtgcgaa cctgcacgct tcgtggacgg cgtgggcgtt ttatgtttgg
    15301 ctcgccattg cctgctggct gattttcttt tccatcatcc atccgcattg gcaatggcag
    15361 gcgctgaaaa tgccgcgttt cagcgcggtg gcggacatca cgatgatcgg cgtgctgacc
    15421 tacctgttcg gcggcatcga ttccggcttc ggcatcctga ttctgccctt cgtcgtctgc
    15481 tcctgcctgc tcagctacgg gcgttacccc ctgctctatg ccagctacgc cgccatcctg
    15541 ctgatattca acgccattgc cgacggcgat atcggcaaat atccgcacat atcggatgcc
    15601 cgaaccgcct cggcaacctt catcctcgtc gccgcctcct atctttccgc catcttcacc
    15661 tcactgtcgg tcagatacat cgacagcgcc ggccaactgg cccgcgacaa ccacctcgcc
    15721 taccggcgca tcaaaggctt gagccaaacc gtactcgaac gcgttcaaga agccgtcgtc
    15781 gtcatcaata ccgaagggct ggcggtgctg ttcaaccgga aggcgaaaga ccttttcccc
    15841 gcactcgaaa tcggacggcg cgccgacctg tccgattctg ccgccgaact gtgggaccaa
    15901 gcctctccgc acactttcga atacgtcctc ggcacactcg gcctgaccgc ccgcatccgc
    15961 gccgtgccga tgaacaaaga gccggacaag ctgcttatcc tctacatccg cccgcaaagc
    16021 gaaattcagg cagaagccct gtccgtcaaa cttgccgcgc tcggacaact gaccgccaac
    16081 cttgcccacg aaatccgcaa cccgatgtcc gccatccgcc acgccaacga cctgctgcgc
    16141 gaaaatatgg aagcgggggc ggcagatccg ttcaacgcca aattgtgcaa aatcatcgac
    16201 ggcaacgtct gccgcatcga caaaatgctc gaagacattt cctcactcaa caagcgcaac
    16261 aaaaccgaac gcgaaaccat cggcctgata ccgttttggg aagaattcaa acaagagttc
    16321 ctgctcggcc atcccgatgc cgccggctgc atccgcttgg atatgcaggg caatcacccg
    16381 accgcctatt tcgatcccgc ccacctgcgg cagattatgt ggaatctcgc caacaacgcg
    16441 tggcggcaca gccgcaaaca gcccggctcg atttccgtca ccatccgccc cgcgcaaaaa
    16501 aacaccgtct gcatcctctt tgccgaccgc ccgaagtgca ggaacacctg ttcgaaccct
    16561 tttacaccac ggcggaaaac ggcaccggcc tcgggctgta tgtcgcccgc gaactggcgc
    16621 acgccaattt cggcgatttg acctacctac cggaagccaa atgtttcgaa ctcacattac
    16681 cggaaaaaac caatgactga actgcaacac cccgtcctcg tcgtcgatga cgaaaccgac
    16741 attctcgacc tgatggaaat gaccctgatg aaaatgggct tgcgcgtcca taccgcgtca
    16801 ggcgttgccg aagccaaaaa caagctcgac agccaacgct attcgctcgt cctgaccgat
    16861 atgcgtatgc cggacggctc ggggctggaa gtcgtccaac acatcaacag ccgcctgctc
    16921 gatacgccgg ttgccgtcat caccgccttc ggcaacgccg atcaggcaca ggaagcgttg
    16981 cgttgcggcg cgttcgaccc cgataccatg cagatacagg actatctcga ccaaatcgaa
    17041 cgcgacatta tcgaacaaac cctcaaacaa accgaaggca accgcacgca ggccgccaaa
    17101 cgcttgggca tcagcttccg ttccatgcgc taccgtatgg aacgcctcaa catcggctga
    17161 cgacaaaacg gcatccgcac catctccgcc cacccgaaaa aatgccgtct gaaacggcac
    17221 gggaaagcgg gttcgcccca cgcccgaacg gacacaaaac accatgaccg acatccttat
    17281 tgacaacacc gccaccgaaa ccgtccgcac cctgatacgg gcattccccc ttgtgcccgt
    17341 ttcccaaccg cccgaacaag gcagttacct ccttgccgaa cacgataccg tcagcctcag
    17401 gcttgtcggg gaaaaaagca gcgtcatcgt cgattttgcc tccggcgcgg cacaataccg
    17461 gcgcacaaaa ggcgggggcg aactcatcgc caaagccgtc aaccacaccg cgcaccccac
    17521 cgtttgggac gcaaccgcag gattggggcg cgacagcttc gtcctcgcct cgctcgggct
    17581 ggccgttacc gccttcgagc aacatcccgc cgtcgcctgc ctgctttcag acggcatccg
    17641 ccgcgccctc ctcaatcccg aaacgcaaga caccgccgcg cgcatcaccc tgcatttcgg
    17701 caacgccgca gtacaaatgc ccgcacttgt tcaaacacaa ggcaaacccg acatcgtcta
    17761 tctcgacccc atgtatcccg aacgccgcaa aagtgccgcc gtcaaaaaag aaatgaccta
    17821 cttccaccgg ctcgtcggtg aagcgcaaga tgaggccgcc ctcctccata ccgcacgcca
    17881 aacggcaaaa aaacgcgtcg tcgtcaaacg cccccgcctc ggcgaacacc ttgccggaca
    17941 agcccctgcc taccaataca ccggcaaaag cacccgcttc gacgtttacc tgccctacgg
    18001 gacggacaag ggataacgcc cataaaacaa gacaccgaaa aatttgccgt tcttatgcaa
    18061 acgagaaacc ggtttttgcg tttcgactgt tttggataaa tcatcacacc ttaaagtttg
    18121 tcattcccac ggaagtggga atccagaaat gaaaagcaac aggaatttat cgtaaatgac
    18181 cgaaaccgaa c
//
LOCUS       DAWFBO010000025        18113 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFBO010000025 DAWFBO010000000
VERSION     DAWFBO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.025"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(84..1187)
                     /locus_tag="WGO87_000605"
     CDS             complement(84..1187)
                     /locus_tag="WGO87_000605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0391998.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1422..2978)
                     /gene="dnaA"
                     /locus_tag="WGO87_000606"
     CDS             complement(1422..2978)
                     /gene="dnaA"
                     /locus_tag="WGO87_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0391999.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPNEPRQAAGSASRPELEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VSASSRFMNRPVIDMDLARTALQDIIAEKHKVITADLIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3271..3405
                     /gene="rpmH"
                     /locus_tag="WGO87_000607"
     CDS             3271..3405
                     /gene="rpmH"
                     /locus_tag="WGO87_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0392000.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3408..3773
                     /locus_tag="WGO87_000608"
     CDS             3408..3773
                     /locus_tag="WGO87_000608"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0392001.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVIGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRPTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3838..4065
                     /gene="yidD"
                     /locus_tag="WGO87_000609"
     CDS             3838..4065
                     /gene="yidD"
                     /locus_tag="WGO87_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0392002.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHGHDPVP"
     gene            4238..5875
                     /gene="yidC"
                     /locus_tag="WGO87_000610"
     CDS             4238..5875
                     /gene="yidC"
                     /locus_tag="WGO87_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0392003.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDETKPFVLFGDGKEYT
                     YVAQSELLDAQGNNVLKGIGFTAPQKQYSLNGDKVEVRLSAPETNGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSAWILQPKGGQSVCAAGDCRIDI
                     KRRNDNLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(5956..6669)
                     /locus_tag="WGO87_000611"
     CDS             complement(5956..6669)
                     /locus_tag="WGO87_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0392004.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6728..7318)
                     /gene="maf"
                     /locus_tag="WGO87_000612"
     CDS             complement(6728..7318)
                     /gene="maf"
                     /locus_tag="WGO87_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0392005.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7466..7969
                     /locus_tag="WGO87_000613"
     CDS             7466..7969
                     /locus_tag="WGO87_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0392006.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSLHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGMLLEKELDLRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            8003..8182
                     /gene="rpmF"
                     /locus_tag="WGO87_000614"
     CDS             8003..8182
                     /gene="rpmF"
                     /locus_tag="WGO87_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0392007.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8357..8884
                     /locus_tag="WGO87_000615"
     CDS             8357..8884
                     /locus_tag="WGO87_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0392008.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            8972..10027
                     /gene="plsX"
                     /locus_tag="WGO87_000616"
     CDS             8972..10027
                     /gene="plsX"
                     /locus_tag="WGO87_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0392009.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTAQIVGMDEAPQSALKNKKDSSMRVAVNQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            10163..10421
                     /locus_tag="WGO87_000617"
                     /pseudo
     CDS             10163..10421
                     /locus_tag="WGO87_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
     gene            10448..10864
                     /locus_tag="WGO87_000618"
     CDS             10448..10864
                     /locus_tag="WGO87_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392010.1"
                     /translation="MKTSTVVFGGFFITDNGERIQIPVLENPGIKEINHFFSISNFEK
                     KAGVLVFRIIPEPEFGDTELTVYFEKGYYLPMIQTILEDGDIEVKNLKTENYSGKARE
                     ILGDSYPIEHTSKNISTIQNIISEFMIQKQTPAPII"
     gene            10884..11087
                     /locus_tag="WGO87_000619"
     CDS             10884..11087
                     /locus_tag="WGO87_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392011.1"
                     /translation="MPSEAAWAFRRHFLRLKQHIGNPAPPVHGNLHLKTICIPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            11114..12076
                     /locus_tag="WGO87_000620"
     CDS             11114..12076
                     /locus_tag="WGO87_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0392012.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            12123..12494
                     /locus_tag="WGO87_000621"
     CDS             12123..12494
                     /locus_tag="WGO87_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ0392013.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            12520..13446
                     /gene="fabD"
                     /locus_tag="WGO87_000622"
     CDS             12520..13446
                     /gene="fabD"
                     /locus_tag="WGO87_000622"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0392014.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFANAVKLVRLRAELMQSAVPQGVGAMAAILGLEDGQVKAICAESAQGEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQIAAFIEAH"
     gene            13585..15450
                     /gene="msbA"
                     /locus_tag="WGO87_000623"
     CDS             13585..15450
                     /gene="msbA"
                     /locus_tag="WGO87_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0392015.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            15550..17115
                     /gene="guaA"
                     /locus_tag="WGO87_000624"
     CDS             15550..17115
                     /gene="guaA"
                     /locus_tag="WGO87_000624"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0392016.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRDIFDDAPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAKEKKLTNAKWLAQGTIYPDVIES
                     AGAKTQKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            17177..17923
                     /gene="fabG"
                     /locus_tag="WGO87_000625"
     CDS             17177..17923
                     /gene="fabG"
                     /locus_tag="WGO87_000625"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0392017.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         4541 a         5496 c         4339 g         3737 t
ORIGIN      
        1 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggtata tctccaatac
       61 gggcatcgtg ttccggaaaa ccgtcaaatc cgcatcggca tcacaatata tttgaaattc
      121 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
      181 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
      241 agttcgccgc cctgataggc gatttcgatt tcttcgcgcg cttcttcctg ctcgttgttg
      301 ctgcacacaa cgctcaacag gccgggttgc aggaacaggc gcgcgccacg gaatttttcg
      361 ttggcaagaa tggacacgcg ttccagcgca cccaaaagtt cggcacggga caaaacgaaa
      421 atcttgtcgt tgtccaaagg aatcacgcgg ttgaaatcgg ggaatttgcc gtcgatgacc
      481 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gtttgtccag cagctcgatt
      541 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
      601 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
      661 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
      721 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
      781 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
      841 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
      901 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagtgcccg taaaatgtcc
      961 tgaaatttct tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca
     1021 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
     1081 atcaggacgt tggacaggat gggcagggtg tggcggcgtt cgacgatgcc ggtaacggct
     1141 tgcagcggct tgagtaggct gtcgcgttcg gcttgtaaaa tcaacatttt cgctcctttg
     1201 aatcggtttg tatagtggat taaatttaaa tcaggacaag gcgacgaagc cgcagacagt
     1261 acaaatagta cgaaaccgat tcacttggta cttcagcacc ttagagaatc gttctctgtg
     1321 agctaaggcg aggcaacgct gtactggttt aaatttaatc cgctatatct ttacccttcg
     1381 gacggcatgg gcaatatcat gtcgtctgaa aacgttttcc atcagttttg aatcagaatc
     1441 agcagtttct cgtaatcctg cgccaactcg gggtcttcct cgcgcagttt cgccaccgcc
     1501 ctgatgccgt gcatgacggt cgtatggtcg cgtccgccga acgaatcgcc gatagacggc
     1561 aggctcaaag tggtcagttc tttggtcagg ctcatggcaa cctgacgcgg acgggcaatg
     1621 ttgcgcgtgc gttttttgcc gagtacgtcg ctgattttga tgcggtaata tttcgccacc
     1681 gcatcaatga tgaggtcggc ggtaatgact ttgtgcttct cggcgataat gtcctgcaac
     1741 gccgtacgcg ccaaatccat atcgatgacg gggcggttca taaagcggct gctcgcgctg
     1801 acgcggttga atgcgccttc aagctcgcgc acattggaac ggatcaggtt ggcaataaac
     1861 aatgccgcct cgtcttcgat gctgataccc gccgcctctg cctttttctg caaaatggcg
     1921 atgcgcattt ccaattcggg cggctcgagt tccaaagtca aaccccaaga gaaacgcgat
     1981 ttgagccggt cgtccatgcc ttcgattttg gcgggtaaaa catcgcaagt gaggatgagc
     2041 tgttttttct cgttgtggaa atggttgtac agatagaaaa actcttccat cgtacggtct
     2101 ttgcctttga tgaactggat atcgtcgata atcagcaggt cgtattgctt gtattgctgc
     2161 ttgaacacgt cgtaggtatt gttgcgaacc gccttcataa agctgcggat gtagtcgtcc
     2221 gaatgcatat agcgcacttt cgcatcggga cggtttttca aaagctcgtt gccgaccgcc
     2281 tgcacaaggt gggttttgcc caaacccgtg ctgccataga ggaagaacgg gttataactc
     2341 tgtcccggac tttccgctat cgcctgcgcg gcggcagctg caaggcggtt gcccttacct
     2401 tcgaccaacg tatcaaacgt gtaatccgga gacaggttgg tttgttcgta acgcgcctct
     2461 tccgcatcac gctgcgcatc cgtccgcgct ttcgcaagca cctccaattc cggacgggaa
     2521 gcagacccgg cagcctgacg cggctcgttc ggcaggtttt tcatacgttc cgccaaaata
     2581 tccgccgccg ttttcgacgc agcgggtttg acaggctctt cagacggcag ctcgtccaac
     2641 agaacctcct gcacaggcat ctcctccgac cccgcgtgca aggacggctc ggcaggttcg
     2701 acagcacctt caaccgccgc catctcataa cgcacgcctt ctcccggttt gaatacgaag
     2761 gcggaacggc cggcagccaa ctcttccctc accgcttcta tttttccggc aaactggctc
     2821 ttgagcatat tgcaggcaaa ctggttcttg ccgtacacca cccatacgcc accctcctca
     2881 ccgacggtaa ggggcgcaat ccattgcgca aactgcccgt gaggcaacat atcgtgaaga
     2941 cggcggaggc acagcggcca aaactctgct aatgtcatgg ataggctcga atcggtaaaa
     3001 atgaaatcga aaacaaagaa aatataatat tttcaaaaag aaaacaaatc tgttgaacac
     3061 acatcggttc aaaatgcagc gaaccgatta taccgactcg ccgatatttt atccacaacc
     3121 cgtgcaaaaa tttatccaca gaaaggcggc agaaatccgc aggcaatcgg gcaatcctcc
     3181 tgcaaagttt ctatattgat tgacaaaagc ggcaaattgg agtgtaattc acggtttaat
     3241 tatctaccca ttctattttt aggaaacatc atgaaacgca cttatcaacc ttccgttacc
     3301 aaacgcaaac gcacccacgg cttcctggtg cgctccaaaa cacgcggcgg ccgcgcagta
     3361 ctggcagcac gccgtgccaa aggccgcaaa cgcctggcag tgtaattttg gactaccgct
     3421 tcggaaggca gtaccgcttg ttaaaaacgg atgatttttc atccgttttt gcattcagaa
     3481 accgccgcag ccgcgacctg ctgcaagttt cgcgctcaaa cggcaacggg ctgggccatc
     3541 cccgcatcgg tctggtgatc ggcaaaaaaa ccgccaaacg cgccaacgaa cgaaattata
     3601 tgaagcgcgt catccgcgac tggttcagat tgaacaaaaa ccggctgccg ccgcaggatt
     3661 tcgtcgtgcg cgtccaccgt aaattcgaca ggcctaccgc aaaacaggca agggcggaac
     3721 tggcacaact catgttcggc aaccccgcaa ccggatgcag gaaacaggca tgatcagaac
     3781 ggtactctgc aggcaaggtt cagacggcaa cgggtttccc atacaaggaa catcccgatg
     3841 aacttcctat tgtccaaact cctgctggga ctgatacggt tctaccaata ttgcatcagc
     3901 ccgctgattc cgccgcgctg ccgttatacg ccgacctgtt cgcaatacgc ggtcgaagcg
     3961 gtcaaaaaat acggcgcatt caaaggcggt cggctcgcca tcaagcgcat tgcacgctgc
     4021 caccctttcg gcggacacgg acacggacac gaccccgttc cctgacccga cgcaatattc
     4081 aaattgcacg ctttcctttt atttcccatc ggtttctata taatgccgtc tgaaccttcg
     4141 ggctggcggc acgaccgtcg ggtatgaagc ccgcccttat tccccgtcta tcggaacacg
     4201 caacctgcgg catttccgac cattcaggaa actctatatg gattttaaaa gactcacggc
     4261 gtttttcgcc atcgcgctgg tgattatgat cggctgggaa aaaatgttcc ccaccccgaa
     4321 acccgtcccc gcaccccaac agacggcaca aaaacaggca gcaaccgctt ccgccgaagc
     4381 cgcgctcgcg cccgcaacgc cgattaccgt aacgaccgac acggttcaag ccgtcattga
     4441 tgaaaaaagc ggcgacctgc gccggctgac cctgctcaaa tacaaagcaa ccggcgacga
     4501 aaccaaaccg ttcgtcctgt ttggcgacgg caaagaatac acctacgtcg cccaatccga
     4561 acttttggac gcgcaaggca acaacgttct gaaaggcatc ggctttaccg caccgcaaaa
     4621 acaatacagc ctcaacggcg acaaagtcga agtccgcctg agcgcgcccg aaaccaacgg
     4681 actgaaaatc gacaaagtct ataccttcac caaaggcagc tatctggtca acgtccgctt
     4741 cgacatcgcc aacggcagcg gtcaaaccgc caacctgagc gcggactacc gcatcgtccg
     4801 cgaccacagc gaacccgagg gtcaaggcta ctttacccac tcttacgtcg gccctgttgt
     4861 ttatacccct gaaggcaact tccaaaaagt cagcttctcc gacttggacg acgatgccaa
     4921 atccggcaaa tccgaggccg aatacatccg caaaaccccg accggctggc tcggcatgat
     4981 tgaacaccac tttatgtccg cttggatttt gcagcctaaa ggcggacaaa gcgtttgcgc
     5041 cgctggcgac tgccgtattg acatcaaacg ccgcaacgac aacctgtaca gcaccagcgt
     5101 cagcgtgcct ttagccgcta tccaaaacgg cgcgaaatcc gaagcctcca tcaacctcta
     5161 cgccggcccg cagaccacat ccgtcatcgc aaacatcgcc gacaacctgc aactggcaaa
     5221 agactacggc aaagtacact ggttcgcttc cccactcttc tggctcctga accaactgca
     5281 caacatcatc ggcaactggg gctgggcaat cgtcgttttg accatcatcg tcaaagccgt
     5341 actgtatcca ttgaccaacg cctcttaccg ctctatggca aaaatgcgtg ccgccgcacc
     5401 caaactgcaa gccatcaaag agaaatacgg cgacgaccgt atggcgcaac aacaggcgat
     5461 gatgcagctt tacacagacg agaaaatcaa cccgctgggc ggctgcctgc ctatgctgtt
     5521 gcaaatcccc gtcttcatcg gattgtattg ggcattgttc gcctccgtag aattgcgcca
     5581 ggcaccttgg ctgggttgga ttaccgacct cagccgcgcc gacccctact acatcctgcc
     5641 catcattatg gcggcaacga tgttcgccca aacttatctg aacccgccgc cgaccgaccc
     5701 gatgcaggcg aaaatgatga aaatcatgcc gttggttttc tccgtcatgt tcttcttctt
     5761 ccctgccggt ctggtattgt actgggtagt caacaacctc ctgaccatcg cccagcaatg
     5821 gcacatcaac cgcagcatcg aaaaacaacg cgcccaaggc gaagtcgttt cctaaatgcc
     5881 gcagcatgaa aaatgccgtc tgaaacctgt tcagacggca tttttattgc tcacccccta
     5941 tcggggcgga aatcttcaac ctgcatacat cacaaaaatc gtcgggcgtt ttttcagatt
     6001 gggcatttct tttctttttc gccactgcac aaccgtctgg ctgatgattt cctgagtcgg
     6061 caacgtcaaa tccgtagccg tgcacaagcg cgtttcagga tgcaggtttt ccaccgcatc
     6121 cgccaacaac acatcgttgc gatacggcgt ttcgataaac agctgcgttt cattctgctg
     6181 gcgcgaacgt tgctccaaag ccttcaaact ctgaatccgc tcgttttttt cagacggcag
     6241 ataaccctta aacgcaaaac tctgcccgtt cgcacccgaa gccatcaaag ccagcagcag
     6301 gctggaaggc ccaaccagcg gacgcacttc aaaaccgtgt ttatgcgcca aagccaccag
     6361 attcgcaccc ggatcggcaa cagccgggca acccgcctcg ctgacaatgc ccatactgcg
     6421 cccttcttgc aaaggtttca gcaattccgg caaagtcttc aaatccgtgt gttcgttcag
     6481 cgtttgcaaa ttcagttcgc ggataggcat tgtcacgccc aaatgtttca aatgcgcgcg
     6541 cgccgttttt tccgcttcca cgacaaaatc cgtcagcccg acaatcgcct gttgttcatg
     6601 cggcaacagg cacggcgtgt caggcgtacc caaaggcgta ggaatcaaat acaaaacagg
     6661 agacaccatt ccctcactca tcggttaaaa atgccgtctg aacccttcag acggcataaa
     6721 cgggcagtta cagaacctcc acgccctcat ttttcaaaaa atcaaccaga cggaaaaccg
     6781 gcaaaccgat taaagcattc ggatcggtac tctcaatcct ttcgatcaac aatgcaccca
     6841 aagcctcgct cttcaacgca cacgagcaat aaaccgcatc aggctcgcgc tccaaatagc
     6901 ggaggatgtg tagctcgtcc aacttcctca tcacgaccac cgtcttatcg atatgccgct
     6961 gcatcctgcc cgtaaccgta tttagcagaa cgaccgcgct gtaaaactca atctccctgc
     7021 cgctcaaatg catcaacatc ttgtgcgcat tagcaaggtt catcggcttg ccccactgcc
     7081 tgccgtcgca ccacgccacc tggtccgcac cgacaatcaa cgcctctggg aaacgcccgg
     7141 ccaacgaccg cgccttaccc tcggcaaggc gcaatgccgt ctgaggggcg gattccccca
     7201 acatcggcgt ttcgtcaaaa tcgggggacg ccgcctgaaa ggcaatgccg agcctttcca
     7261 tctgttcgcg gcggaaaacc gaactcgtac ccaaaatcaa aggcagttcc aaacccatcc
     7321 catcctcctt accgttgaaa acacgcccga aggggcggta aaatccagcc atgcgccgaa
     7381 acacggatac acgccttcgg cgtaccgcaa catttttctt aaaaatattg acgttagaac
     7441 atctaaatta tatcatatct cgtttatgtc agaccctaat ttgattgacc cggaaatttt
     7501 cgccgccgaa gggcagaacc tgcaaggcag ttttctgctg gaagaattgg acgagcgagt
     7561 cagtttgcac gattatcccg ccgacaggca gaccaaaata tcgtttacac tgaccggcgg
     7621 tcgcgaccgg ctgcaacgcc tgttcctcga cctgaacgtc aaagccgata tgcccctgat
     7681 ttgccagaga tgtatcaaac ccatgccgtt catgcttgat gaaagcagcc gtatcgtcct
     7741 gttttccaac gaagagtcct tggacgaatc catgcttgcc gatgaagaat tggaaggtat
     7801 gctgctcgaa aaagagcttg acttgcggac cttggtcgaa gaccaaatcc tgatgtcgct
     7861 ccctttctcc ccccggcacg aagactgcgg cgacaatggg acactggaag aagtcaatcg
     7921 ggacaaacca aacccctttg ctgttttggc aggtttgaaa agcagttaat taggacacag
     7981 tttatttatc taggagcttg aaatggccgt tcaacaaaac aaaaaatccc cttccaaacg
     8041 cggtatgcac cgttcgcacg acgcgctgac cgcgcctgca ctgtctgtcg acagcacaac
     8101 cggcgaagta caccgcccgc accacatctc ccccaacggt atgtaccgcg gccgcaaagt
     8161 ggtcaaagcc aaaggcgaat aatccctatt cgactgactg aaaaagccag aacattgcca
     8221 tgcaattact ggcttttttt gcattggacg caccatccgt ccaaactttc gccatacgtc
     8281 aacacacagg ggcaaagcgt tccgtataat accccgtgaa aatattccaa aagccccaac
     8341 caccaaggaa attccgatga aacagaaaat ctggtacacc tacgatgaca tccaccgcgt
     8401 catcaaagca ttggcagaaa aaatccggaa cgccgacatc aaatacgatg ccatgattgc
     8461 catcggcggc ggcggcttta ttccggcacg tatgctgcgc tgttttctgg aaattccgat
     8521 ttatgccgta accaccgcct attacgacag cgacaacgaa ggacaggtta cagaagaagt
     8581 caaaaaagtc caatggctcg accccgttcc cgaagccctg cggggcaaaa acgtccttgt
     8641 tgtcgatgaa gtggacgaca gccgcgtaac catggaattt tgcctgacag agctgcttaa
     8701 agaagacttc ggcaccatcg gcgtcgccgt actgcacgaa aaaatcaaag ccaaagcagg
     8761 caaaatcccc gaaggcattc cctatttcag cggcatcacc gtagaagact ggtggatcaa
     8821 ctatccatgg gacgcactcg acatcgacga acacaaccgc cttgccgagg ccggccgagg
     8881 ctgacccttt cagacggcat attttccgaa ccgatgccgt ctgaagcccg cacgacccct
     8941 gccgcagacc gaaaacctac cggagaaccc tatgattaca cttgccgtag atgccatggg
     9001 cggcgaccaa ggacttgccg ttaccgtacc cggcgcaacc gcattcctcc aagcacaccc
     9061 cgatgtccgc ctgattatga ccggcgacga aacgcaactg cgccaagccc tgaccgcggc
     9121 aggcgcaccg atggaacgca tcgacatctg ccacaccgca caaatcgtcg gcatggacga
     9181 agcgccgcaa tccgccctga aaaacaaaaa agactcctcc atgcgcgttg ccgtcaacca
     9241 agttaaagaa ggcaaagccc aagccgccgt atccgcaggc aacacgggtg cgctcatggc
     9301 aaccgcacgt ttcgtcctca aaaccattcc cggcatcgaa cgccccgcca tcgccaaatt
     9361 ccttccttcc gacaccgacc atgttaccct tgcactcgac cttggcgcga acgtcgactg
     9421 cacgcccgaa cagctcgccc aatttgccgt tatcggcagc gaactcgtcc acgcactcca
     9481 tcctcaaaaa ggacagccgc gcgtcgggct ggtcaacgtc ggcacggaag acatcaaagg
     9541 tacggacacc gtcaaacaaa cctacaaact gctgcaaaac agcaaactca actttatcgg
     9601 caacatcgaa agcaacggca tcctctacgg cgaagcagat gtcgtcgtcg ccgacggctt
     9661 tgtcggcaac gtcatgctca aaaccatcga aggcgcggtc aaattcatga gcggagccat
     9721 ccgccgcgaa ttccaaagca acctgttcaa caaacttgcc gccgttgccg ccctacccgc
     9781 cctcaaaggg ctgaaaaaca aactcgatcc gcgcaaattc aacggggcca tcctgctcgg
     9841 gctgcgcggc atcgtgatta aaagccacgg cggcacagac gaaaccggtt tccgctacgc
     9901 actcgaagaa gcctaccacg aagccaagtc agccggcctt tccaaaatcg aacagggcgt
     9961 agccgaacaa ctcgccgcac tcgaaactgc caaagccgtc caaaacgaaa atgtcggcgg
    10021 tctgtaacac gcgcgatgcc gtccgaacgc ccccgcccct ttcagacggc atccgcccgc
    10081 cccgaacctg cgggcgcgga cggcgatgca cctgtccggc acttcccaaa tacttccctg
    10141 taaaataagg agtatttgaa aaatgaagac attagaaaaa cggatgaggg cattagacaa
    10201 acggataatg aagttcggaa aatcccttga aggcaggctt gaggcttgat gcccgtctga
    10261 ttgaatccgc attggattat attcattatt cggaacgttt tttggctttt gaaatcctgt
    10321 gtacttatat cgaagatttc gatgtccggc tgacggaaca agaatcccgg gaaatttctt
    10381 ttatcaacaa ggaatttgag atagaaagca cgtccgatta accaataaag ccaatgggtt
    10441 gataaacatg aaaacatcaa cggttgtttt tggcggattt tttattacag ataatggaga
    10501 acgaatccaa atccccgttt tggaaaatcc tggcattaaa gaaatcaatc actttttttc
    10561 catatcaaat tttgagaaaa aagccggcgt ccttgttttc agaatcatcc ccgagccgga
    10621 atttggcgat accgaattaa ctgtctattt tgaaaaagga tattatttgc caatgattca
    10681 aacaatttta gaagacgggg atattgaagt caaaaacctt aaaacggaaa attacagtgg
    10741 gaaggcaagg gaaattttgg gagattccta ccctattgag catacctcta aaaatatttc
    10801 tacaattcaa aatataattt ctgaatttat gatacaaaaa caaacaccgg caccaataat
    10861 ttgatggaaa cccaacaatc aaaatgccgt ctgaagcagc ttgggctttc agacggcatt
    10921 tcctccgctt aaaacagcat atcggcaacc ccgccccgcc cgtccacggc aatctgcatc
    10981 tgaaaaccat ctgtatccca aaccacaccc ccatcccgat ttccgtcatt tgcaccctgc
    11041 ccgtattggg caatcatcgg tttttcgctt acaatagccg aatctgaacc aactatccaa
    11101 aaaggccgtt cccatgcagt atgccaaaat ttccggcacg ggcagctatc ttcccgccaa
    11161 ccgcgtcagc aatgacgacc ttgcccaaaa agtggatacc tcggacgagt ggattaccgc
    11221 gcgcacgggc atcaaattcc gccatattgc agccgaaaat gagaaaacca gcgatcttgc
    11281 cgccgaagca gcccgccgcg cactggatgc agccggatta gacagcggcg aaatcgattt
    11341 aattattgtg gcgacggcaa cgccggatat gcagtttcct tctaccgcca ccatcgtcca
    11401 acaaaaactg ggcatcacca acggctgccc cgcgtttgac gtacaggcgg tgtgtgcggg
    11461 ctttatgtac gcgctgacca cggcaaacgc ctacatcaaa agcggcatgg caaaaaacgc
    11521 gctggtcatc ggcgcggaaa ctttcagccg catcgtcgat tggaacgacc gcacaacctg
    11581 cgtattgttc ggcgacggcg cgggcgcggt ggttttgagc gcggcggaca aaccgggcat
    11641 catccacagc aaactcaaag ccgacggcaa ttatctgaaa ctcttaaacg tccccgggca
    11701 aatcgcctgc ggcaaagttt ccggttcgcc gtacatttcg atggacggtc ccggcgtgtt
    11761 caagtttgcc gtcaaaatgc tgtccaaaat cgccgatgac gttatcgaag aagccggtta
    11821 taccgccgct caaatcgact ggatcgtgcc gcatcaggca aaccgccgca ttatcgaatc
    11881 gaccgcgaaa catttaggtt tgagtatgga caaagtcgtc ctgaccgtcc aagaccacgg
    11941 caacacatcc gccgcatcga ttccgctggc tttggatacg ggcatccgca gcggacaaat
    12001 caaacgcggt caaaacctgc tgctcgaagg catcggcggc ggtttcgcgt ggggcgcggt
    12061 gctgttgcaa tattgaaccc gatgccgtct gaaacaggct ttcagacggc atttcccata
    12121 tcatgaagcg gcaggctttc ttcaaaccaa ttgcgtgtgc ggcatttctg tctgccgttt
    12181 cgctgcgcct ccccgtattg ggcgcgtgtt acgcaatatt gtccctctat gcgtttgcac
    12241 tttacggcat cgacaaacgg cgtgccgtgc ggggaaaacg ccgcattccc gaacaccgcc
    12301 tgctgctgcc tgccttgttc ggcggttggg cgggcgcata cttgggcagc aggatattca
    12361 ggcataaaac ggcgaaaaag cgttttgttg tgctgttccg tctgactgtt tcgggcaatg
    12421 tcctggcgac ctgcatcctg attgattatt tcgttccgcc cgaacttttt gtcaaactcg
    12481 ggcaacatct ctgacaatcc aaccaaacaa aggaatacca tgtcttttgc attcttcttt
    12541 cccggacaag gttctcaaag cctcggcatg atgaacggct ttacagggca ggccgtcgtc
    12601 aaaaacacat ttgacgaagc ctccgccata ttggggcagg acttgtgggc gatgattaac
    12661 ggcagcgatg ccgaaatcat cggtcaaacc gtcaataccc aacccatcat gctcgccgcc
    12721 ggcgttgccg tttaccgcgc ctatttggaa gcgggtggca aaacgcctgc cgccgttgcc
    12781 ggacacagcc tcggcgaata taccgcgctt gtcgccgccg gcgcattgga ttttgccaac
    12841 gcggtcaaac tcgtgcgcct acgcgcagaa ctgatgcagt ccgccgtacc gcaaggcgtg
    12901 ggcgcaatgg cggcgattct cggcttggaa gatgggcagg ttaaagccat ttgtgccgaa
    12961 tccgcacagg gcgaagtggt cgaagccgtc aacttcaact cgcccgggca aatcgtgatt
    13021 gcaggcaacg ccgccgccgt cggacgcgcc atggctgccg ccaaagaagc cggagccaaa
    13081 cgcgccctgc cgctgcccgt gtccgtacct tcccattgca gcctcatgaa acccgccgcc
    13141 gacaaacttg ccgaagcctt gaaaaccgtt gaaatcaagc agccgcaaat ccgcgttatc
    13201 cacaacgccg acgttgccgc ctacgatgat gccgacaaaa tcaaagacgc gctcgtccgc
    13261 cagctttaca gccccgtacg ctggacggaa accgtcaacg ccctcgtttc agacggcatt
    13321 gccgaatccg ccgaatgcgg cccgggcaaa gttctggcag gtctggcaaa acgcatcaac
    13381 aaagccgccg cgtgcagcgc actgaccgat gccggacaga ttgccgcctt tatagaagca
    13441 cactgacttc gttctgtaaa aagcagcctg catgcctcag gctgcttttc acgtccgaac
    13501 ggcaaacagc cgcatatttg cgctataatt gatgacgacc aaaccaccga cagcggctgc
    13561 cgttgcagtt cccgccccac cgacatgata gaaaaactga ctttcggact gtttaaaaaa
    13621 gaagacgcgc gcagctttat gcgcctgatg gcgtacgtcc gcccctacaa aatccgcatc
    13681 gttgccgccc tgattgccat tttcggcgtt gccgccaccg aaagctacct tgccgccttc
    13741 atcgcccccc tgattaacca cggcttttcc gcacctgccg cgccgcccga gctgtctgcc
    13801 gccgccggca tcatttccac cctgcaaaac tggcgcgaac agtttaccta tatggtttgg
    13861 gggacggaaa acaaaatctg gaccgtcccg ctctttctca tcattctcgt cgtcatccgt
    13921 ggcatctgcc gctttaccag cacctatctg atgacttggg tctccgtgat gaccatcagc
    13981 aaaatccgca aagatatgtt tgccaaaatg ctgacccttt cctcccgcta ccatcaggaa
    14041 acgccgtccg gcaccgtact gatgaatatg ctcaacctga ccgaacagtc ggtcagtaac
    14101 gccagcgaca tcttcaccgt cctcacgcgc gacacgatga tcgttaccgg cctgaccatc
    14161 gtcctgcttt acctcaactg gcagctcagc ctcatcgttg tcctgatgtt ccccctgctc
    14221 tccctgctct cgcgctacta ccgcgaccgt ctgaagcacg tcatttccga ctcgcaaaaa
    14281 agcataggca cgatgaacaa cgtgattgcc gaaacccatc agggacaccg cgtcgtcaag
    14341 ctgttcaacg ggcaggcgca ggcggcaaac cggtttgacg cggtcaaccg caccatcgtc
    14401 cgcctcagca aaaaaatcac gcaggcaacg gcggcacatt ccccgttcag cgaactgatt
    14461 gcctcgattg ccctcgccgt tgtcatcttc atcgccctgt ggcaaagcca aaacggctac
    14521 accaccatcg gcgaatttat ggcattcatc gtcgcgatgc tgcaaatgta cgcacccatc
    14581 aaaagccttg ccaacatcag catccctatg cagacgatgt tcctcgccgc cgacggcgta
    14641 tgtgcatttc tcgacacccc gcccgaacag gacaaaggca cgctcgcgcc gcagcgtgtc
    14701 gaagggcgta tcagcttccg caacgtcgat gtcgaatacc gttcggacgg catcaaagcc
    14761 ctcgacaact tcaacctcga catcagacaa ggcgaacgcg tcgccctggt cggacgttcc
    14821 ggcagcggca aatccaccgt cgtcaacctg ctgccccgct ttgtcgaacc gtctgccggc
    14881 aacatctgca tagacggtat cgacatcgcc gacatcaaac tcgactgcct gcgcgcccaa
    14941 ttcgccctcg tctcccaaga cgtattcctg tttgacgaca ccctgtttga aaacgtccga
    15001 tacagccgtc ccgacgcagg cgaagccgaa gtcctgtccg ccctccaagc cgccaacctg
    15061 caaagcctga ttgacagctc cccgctcgga ctgcaccagc ccatcggatc gaacggcagc
    15121 aacttgtccg gcggacagcg gcaacgcgtc gccatcgccc gcgccatttt gaaagacgcg
    15181 ccgatattat tattggacga agccaccagc gcattagaca acgaatccga acgcctcgtc
    15241 caacaagcgc tcgaacgcct gatggaaaac cgcaccggca tcatcgtcgc ccaccgcctg
    15301 accaccatcg aaggggccga ccgcatcatc gtaatggacg acggcaaaat catcgaacaa
    15361 ggcacacacg aacaactgat gtcccaaaac ggctactaca cgatgttacg caatatctca
    15421 aacaaagatg ccgccgtccg gacggcataa acaaaatgcc gtccgaaatg gtataatcgc
    15481 cccgaccctt tcagacggca ctatatccgc cgacccatcc gattatcttc aatcactgta
    15541 aaacccatta tgacccaaga caaaatcctc atcctcgact tcggttcgca agttacccag
    15601 ctgattgccc gccgcgtgcg cgaagcccac gtttactgcg aactgcattc cttcgatatg
    15661 cctttggacg aaatcaaagc cttcaacccc aaaggcatca tcctttccgg cggccctaat
    15721 tccgtttacg aatccgacta tcaggccgat accggtattt ttgatttggg cattccggtt
    15781 ttgggcatct gctacggaat gcagtttatg gcgcaccact tgggcggcga agtgcagccc
    15841 ggcaaccagc gcgaattcgg ttacgcgcaa gtcaaaacca tcgacagcga actgacccgc
    15901 gatattttcg acgacgcgcc caacacactc gacgtatgga tgagccacgg cgacaaagtg
    15961 tccaaactgc ccgacggctt cgccgtcatc ggcaacaccc cgtcctgccc gattgccatg
    16021 atggaaaacg ccgaaaaaca attctacggc atccagttcc accccgaagt tacccacacc
    16081 aaacaaggcc gcgccctgtt gaaccgcttt gtcttggata tttgcggcgc gcaaccgggc
    16141 tggacgatgc cgaactacat cgaagaagcc gttgccaaaa tccgcgaaca ggtcggcagc
    16201 gacgaagtga ttttaggttt gtcaggcggc gtagactctt ccgtagccgc cgcgctgatt
    16261 caccgcgcca tcggcgacca actgacctgc gtgttcgtcg atcacggttt gttgcgcctg
    16321 aacgaaggca aaatggtgat ggatatgttc gcccgcaact tgggtgtgaa agtgattcac
    16381 gtcgatgccg aagggcagtt tatggcgaaa ctcgccggcg ttaccgaccc tgagaaaaaa
    16441 cgcaaaatca tcggcgcgga atttatcgaa gtatttgatg ccaaagagaa aaaactcacc
    16501 aacgccaaat ggctggcgca aggcacgatt taccccgacg taatcgaatc cgcaggcgcg
    16561 aaaacccaaa aagcccacgc catcaaatcg caccacaacg tcggcggtct gcccgaaaat
    16621 atgaagctca agctgcttga gccgttgcgc gacttgttta aagacgaagt gcgcgaactg
    16681 ggcgttgctt tgggtctgcc gcgcgaaatg gtgtaccgcc atccgttccc cggccccggt
    16741 ttgggcgtgc gcatcttggg cgaagtgaaa aaagaatacg ccgacttatt gcgtcaagca
    16801 gacgatattt tcatccaaga attacgcaac accaccgacg aaaacggcac atcttggtac
    16861 gacctgacca gccaggcatt tgccgtattc ctgcctgtga aatccgtcgg cgtaatgggc
    16921 gacggacgca cttacgacta cgtcgtcgca ctgcgcgcag tcatcaccag cgacttcatg
    16981 accgcacact gggcagaact gccttactca ctgctcggac gcgtgtccaa ccgcatcatc
    17041 aacgaagtca aaggcatcaa ccgcgtggtg tacgatgtca gcggcaaacc gcctgccacc
    17101 atcgagtggg aataaccagc aaacagggct gcaccgtccg gcgcagtctt tcgattatcg
    17161 gaaaaaagga aaaaatatga gcacacaaga tttaagcggc aaaatcgctt tggtaaccgg
    17221 cgcatcgcgc ggtatcggtg cagcaattgc cgacacactg gcggcagccg gtgccaaagt
    17281 catcggtacg gcgaccagtg agagcggtgc ggcggcgatt agcgagcggt tggcgcaatg
    17341 gggcggcgaa ggccgcgtat taaattccgc cgaacccgaa accgtcgaaa acctgattgc
    17401 cgacatcgaa aaaacgttcg gcaaactcga tattctggtc aacaacgccg gcatcacccg
    17461 cgacaacctc ctgatgcgca tgaaagaaga agagtgggac gacatcatgc aggtcaacct
    17521 caaatccgtg ttccgcgctt ctaaagccgt cttgcgcggc atgatgaagc agcgcgccgg
    17581 ccgtatcatc aacatcacat ccgtcgtcgg cgtgatgggc aatgccggtc aaaccaacta
    17641 tgccgcggca aaagcaggct taatcggttt ctccaaatcc atggcgcgcg aagtcggcag
    17701 ccggggcatt accgtcaact gcgtcgcccc cggctttatc gataccgata tgacccgcgc
    17761 cctgccggaa gaaacccgcc aagcctttac cgcccaaacc gccttgggca gattcggcga
    17821 cgcgcaagac attgccgatg cggttttgtt cctcgcttcc gatcaggcga aatacattac
    17881 cggccagacg ctgcacgtca acggcggcat gctgatgccc tgacggacag ccgtgccgac
    17941 aatgccgtct gaagcccttt cagacggcat ttgcattccc aaacaaaatg aacacacacc
    18001 acaccctgcc ctgcccctgc ggttcaggca caagctgaga cctttgcaaa actcctttcc
    18061 ctcccgacag ccgaaaccca aacacaggtt ttcgtctatt ttcgccccaa ata
//
LOCUS       DAWFBO010000026        16908 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFBO010000026 DAWFBO010000000
VERSION     DAWFBO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.026"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            1..168
                     /locus_tag="WGO87_000626"
                     /pseudo
     CDS             1..168
                     /locus_tag="WGO87_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            412..1056
                     /gene="rplC"
                     /locus_tag="WGO87_000627"
     CDS             412..1056
                     /gene="rplC"
                     /locus_tag="WGO87_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0392018.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1056..1676
                     /gene="rplD"
                     /locus_tag="WGO87_000628"
     CDS             1056..1676
                     /gene="rplD"
                     /locus_tag="WGO87_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0392019.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1673..1987
                     /gene="rplW"
                     /locus_tag="WGO87_000629"
     CDS             1673..1987
                     /gene="rplW"
                     /locus_tag="WGO87_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0392020.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1993..2826
                     /gene="rplB"
                     /locus_tag="WGO87_000630"
     CDS             1993..2826
                     /gene="rplB"
                     /locus_tag="WGO87_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0392021.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2833..3111
                     /gene="rpsS"
                     /locus_tag="WGO87_000631"
     CDS             2833..3111
                     /gene="rpsS"
                     /locus_tag="WGO87_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0392022.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3120..3449
                     /gene="rplV"
                     /locus_tag="WGO87_000632"
     CDS             3120..3449
                     /gene="rplV"
                     /locus_tag="WGO87_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0392023.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3459..4151
                     /gene="rpsC"
                     /locus_tag="WGO87_000633"
     CDS             3459..4151
                     /gene="rpsC"
                     /locus_tag="WGO87_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0392024.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4135..4551
                     /gene="rplP"
                     /locus_tag="WGO87_000634"
     CDS             4135..4551
                     /gene="rplP"
                     /locus_tag="WGO87_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0392025.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4551..4742
                     /gene="rpmC"
                     /locus_tag="WGO87_000635"
     CDS             4551..4742
                     /gene="rpmC"
                     /locus_tag="WGO87_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0392026.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4742..5005
                     /gene="rpsQ"
                     /locus_tag="WGO87_000636"
     CDS             4742..5005
                     /gene="rpsQ"
                     /locus_tag="WGO87_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0392027.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5227..5595
                     /gene="rplN"
                     /locus_tag="WGO87_000637"
     CDS             5227..5595
                     /gene="rplN"
                     /locus_tag="WGO87_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0392028.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5607..5930
                     /gene="rplX"
                     /locus_tag="WGO87_000638"
     CDS             5607..5930
                     /gene="rplX"
                     /locus_tag="WGO87_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0392029.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5940..6479
                     /gene="rplE"
                     /locus_tag="WGO87_000639"
     CDS             5940..6479
                     /gene="rplE"
                     /locus_tag="WGO87_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0392030.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6482..6787
                     /gene="rpsN"
                     /locus_tag="WGO87_000640"
     CDS             6482..6787
                     /gene="rpsN"
                     /locus_tag="WGO87_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0392031.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6803..7195
                     /gene="rpsH"
                     /locus_tag="WGO87_000641"
     CDS             6803..7195
                     /gene="rpsH"
                     /locus_tag="WGO87_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0392032.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7209..7742
                     /gene="rplF"
                     /locus_tag="WGO87_000642"
     CDS             7209..7742
                     /gene="rplF"
                     /locus_tag="WGO87_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0392033.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7756..8109
                     /gene="rplR"
                     /locus_tag="WGO87_000643"
     CDS             7756..8109
                     /gene="rplR"
                     /locus_tag="WGO87_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0392034.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8128..8646
                     /gene="rpsE"
                     /locus_tag="WGO87_000644"
     CDS             8128..8646
                     /gene="rpsE"
                     /locus_tag="WGO87_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0392035.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8639..8824
                     /gene="rpmD"
                     /locus_tag="WGO87_000645"
     CDS             8639..8824
                     /gene="rpmD"
                     /locus_tag="WGO87_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0392036.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8826..9260
                     /gene="rplO"
                     /locus_tag="WGO87_000646"
     CDS             8826..9260
                     /gene="rplO"
                     /locus_tag="WGO87_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0392037.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9272..10582
                     /gene="secY"
                     /locus_tag="WGO87_000647"
     CDS             9272..10582
                     /gene="secY"
                     /locus_tag="WGO87_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0392038.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10587..10805
                     /gene="infA"
                     /locus_tag="WGO87_000648"
     CDS             10587..10805
                     /gene="infA"
                     /locus_tag="WGO87_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0392039.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10826..10939
                     /gene="rpmJ"
                     /locus_tag="WGO87_000649"
     CDS             10826..10939
                     /gene="rpmJ"
                     /locus_tag="WGO87_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0392040.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            11005..11367
                     /gene="rpsM"
                     /locus_tag="WGO87_000650"
     CDS             11005..11367
                     /gene="rpsM"
                     /locus_tag="WGO87_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0392041.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11387..11782
                     /gene="rpsK"
                     /locus_tag="WGO87_000651"
     CDS             11387..11782
                     /gene="rpsK"
                     /locus_tag="WGO87_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0392042.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11802..12422
                     /gene="rpsD"
                     /locus_tag="WGO87_000652"
     CDS             11802..12422
                     /gene="rpsD"
                     /locus_tag="WGO87_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0392043.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12448..13434
                     /gene="rpoA"
                     /locus_tag="WGO87_000653"
     CDS             12448..13434
                     /gene="rpoA"
                     /locus_tag="WGO87_000653"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0392044.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13458..13826
                     /gene="rplQ"
                     /locus_tag="WGO87_000654"
     CDS             13458..13826
                     /gene="rplQ"
                     /locus_tag="WGO87_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0392045.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13973..14686
                     /gene="minC"
                     /locus_tag="WGO87_000655"
     CDS             13973..14686
                     /gene="minC"
                     /locus_tag="WGO87_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0392046.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14715..15530
                     /gene="minD"
                     /locus_tag="WGO87_000656"
     CDS             14715..15530
                     /gene="minD"
                     /locus_tag="WGO87_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0392047.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15534..15797
                     /gene="minE"
                     /locus_tag="WGO87_000657"
     CDS             15534..15797
                     /gene="minE"
                     /locus_tag="WGO87_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0392048.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15801..16721
                     /gene="oxyR"
                     /locus_tag="WGO87_000658"
     CDS             15801..16721
                     /gene="oxyR"
                     /locus_tag="WGO87_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0392049.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16718..>16908)
                     /locus_tag="WGO87_000659"
     CDS             complement(16718..>16908)
                     /locus_tag="WGO87_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392050.1"
                     /translation="LIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNIL
                     AYRVVGSGKWRFENNACA"
BASE COUNT         4623 a         3202 c         4202 g         4881 t
ORIGIN      
        1 cgcctacccg ctgttgtcca tgttcaaagc cgttctgctc ggacaatggc acagcctctc
       61 cgatcccgaa ctcgagcaca gcctcatcac ccgcatcgat ttcaacctgt tttgccgctt
      121 tgacgaactg agcatccccg attacagtca tcaaccatat tccggtttgt cggagaaaga
      181 tgcatacgct gtgatgaccg gataccgacc ctttaaaaga gtctgaccct atgccgtctg
      241 aaaattcaaa acgcttcaga cggcatattg aagatatttc tgatatttct gttgatattt
      301 ctttaacttg tcagatataa tgccgagctt ggtacatttg tgccaagttt aactttgtct
      361 gaaagacagg ccaatcgtag cctgtccctt tactttaaaa ggaaaataat catgacttta
      421 ggtctggttg gacgcaaagt tggtatgacc cgcgtgttcg acgaacaggg tgtttctgtt
      481 ccggtaaccg ttttggatat gtctgccaac cgcgttacac aagtaaaatc caaagatact
      541 gacggctata ctgccgttca agttaccttt ggtcagaaaa aagccaatcg tgtcaacaaa
      601 gccgaagccg ggcactttgc aaaagcaggt gttgaagccg gtcgcggttt gattgagttt
      661 gctttgactg aagaaaaact ggctgaattg aaagctggtg acgaaatcac cgtttctatg
      721 tttgaagtcg gtcaactggt cgatgtaacc ggtacctcta aaggtaaagg tttctccggc
      781 acgattaaac gtcataactt cggcgcccaa cgtacttccc acggtaactc ccgctctcac
      841 cgcgttccag gttctatcgg tatggcacaa gacccgggtc gcgtgttccc cggtaagcgc
      901 atggccggcc aatacggcaa caccaaggca actgttcaaa aattggaagt tgtccgtgtt
      961 gatgcagaac gccaactgct gttggttaag ggtgctgttc cgggtgcggt caacagcgat
     1021 gttgtagttc gtcccagcgt gaaagtaggt gcgtaatgga attgaaagta attgacgcta
     1081 aaggacaagt ttcaggcagc ctgtctgttt ctgatgcttt gttcgcccgc gaatacaatg
     1141 aagcgttggt tcatcagctg gtaaatgcct acttggcaaa cgcccgctcc ggtaaccgcg
     1201 ctcaaaaaac ccgtgccgaa gtaaaacact caaccaaaaa accatggcgt caaaaaggta
     1261 ccggccgtgc ccgttccggt atgacttctt ctccgctgtg gcgtaaaggt ggtcgcgcgt
     1321 tcccgaacaa acccgacgaa aacttcactc aaaaagtaaa ccgcaaaatg taccgtgccg
     1381 gtatggcgac tattctgtcc caattgactc gtgacgagcg tttgtttgcg attgaggcgt
     1441 tgactgccga aactcctaaa accaaagttt ttgctgaaca agtgaaaaat ctgggtctag
     1501 agcaagtgtt gtttgtaacc aaacagctcg acgagaatgt ttacttggct tcacgcaact
     1561 tgccaaacgt gttggttttg gaagctcaac aagttgatcc ttacagcttg ctgcgttaca
     1621 aaaaagtaat catcactaaa gatgcagttg cacaattaga ggagcaatgg gtatgaatca
     1681 acaacgtttg actcaagtga ttttggcacc tatcgtttct gaaaaaagca acgtattggc
     1741 tgaaaaacgc aaccaaatga cgtttaaagt tttggcaaat gcaaccaaac ctgaaatcaa
     1801 agcggctgtt gagctgctgt ttggtgttca agttgcttct gtaactaccg ttacaactaa
     1861 aggcaaaact aagcgttttg gtcgtacttt gggccgccgc agcgatgtta aaaaagctta
     1921 tgtaagcttg gctgccggtc aagagttgga tttggaagcc gctgctgcag ctgcagataa
     1981 ggaataaaca aaatggcaat cgttaaaatg aagccgacct ctgcaggccg tcgcggcatg
     2041 gttcgcgtgg taacagaagg tttgcacaaa ggtgcgcctt atgcaccttt gctcgaaaag
     2101 aaaaattcta ctgccggtcg taacagtaat ggtcatatca ccacccgtca caaaggcggc
     2161 ggtcataaac accattaccg tgttgtagac tttaaacgta acaaagacgg cattcctgct
     2221 aaggtagagc gtatcgaata cgatcctaac cgtactgcct tcatcgcact gttgtgctat
     2281 gcagacggcg agcgtcgtta catcatcgct cctcgcggta ttcaagtcgg tgccgtattg
     2341 gtttccggtg ctgaagctgc catcaaagta ggcaacaccc tgccgatccg caacattccc
     2401 gttggtacga ctatccactg tatcgaaatg aaacccggta aaggtgctca aatcgcacgt
     2461 tctgccggtg cttctgcggt attgctggct aaagaaggcg cgtacgctca agtccgtctg
     2521 cgctctggcg aagttcgtaa aatcaacgta aattgccgtg cgaccatcgg tgaagttggt
     2581 aacgaagagc aaagcctgaa aaaaatcggt aaagccggtg ccaaccgttg gcgcggtatt
     2641 cgtccgaccg tacgcggtgt tgtcatgaat cctgtcgatc acccgcatgg tggtggtgaa
     2701 ggccgtacgg gcgaggcccg cgaaccggtc agcccatggg gtactcctgc taaaggctac
     2761 cgcactcgta ataacaaacg cacggataac atgattgttc gtcgccgtta ctcaaataaa
     2821 ggttaattta gtatggctcg ttcattgaaa aaaggcccat atgtagacct gcatttgctg
     2881 aaaaaagtag atgctgctcg cgcaagcaac gacaaacgcc cgattaaaac ctggtctcgt
     2941 cgttctacca ttctgcctga ttttatcggt ctgaccattg ctgtgcacaa cggccgcacc
     3001 catgtgcctg tgtttatcag cgacaatatg gttggtcata aattaggcga attctcattg
     3061 acccgtacct ttaaaggcca cttggccgat aaaaaggcta aaaagaaata aggtgaatca
     3121 tgagagtaaa tgcacaacat aaaaatgccc gtatctctgc tcaaaaggct cgtttggtag
     3181 ctgatttgat tcgtggtaaa gacgttgccc aagctttgaa tattttggct ttcagtccta
     3241 aaaaaggtgc cgagctgatt aaaaaagtat tggagtcagc tattgctaat gccgagcaca
     3301 ataacggtgc ggacattgat gaactgaaag tggtaactat ctttgttgac aaaggcccaa
     3361 gcttgaaacg ttttcaagct cgcgccaaag gtcgcggtaa ccgcatcgaa aaacaaactt
     3421 gtcatatcaa tgtgacagtg ggtaactaag gaaaagctat gggacaaaag attaacccta
     3481 caggctttcg cctggcggta actaaagact gggcttcaaa atggtttgct aaaagcaccg
     3541 acttttctac tgttttgaag caggatatcg atgttcgcaa ttatttgcgt caaaaattgg
     3601 ccaatgcttc ggttggtcga gtggttattg aacgccctgc aaaatctgca cgcattacca
     3661 ttcactccgc tcgtccgggt gtggttatcg gtaaaaaagg tgaggatatc gaggttttga
     3721 aacgtgactt gcaagtcttg atgggtgtac ctgttcatgt aaatattgaa gagattcgcc
     3781 gtcctgagtt ggatgctcaa attattgctg acggtattgc ccagcagttg gaaaagcgcg
     3841 ttcaattccg tcgtgctatg aaacgagcaa tgcaaaatgc aatgcgttct ggtgctaaag
     3901 gcattaagat tatgacttca ggccgtctga atggtgcgga tattgcccgt agcgaatggt
     3961 atcgtgaagg tcgtgtgcca ctgcatactt tacgtgcaaa tgtagattat gcaaccagcg
     4021 aagcgcacac cacatatggt gtattgggtc tgaaagtttg ggtttatacg gaaggcaata
     4081 ttaaatcttc caaacctgaa catgagagta aacaaagaaa ggcaggtaga cgtaatgctg
     4141 cagccaacta gactgaaata ccgtaagcaa caaaagggtc gcaatactgg catcgctact
     4201 cgcggtaata aggtaagttt cggtgagttc ggcttgaaag ccgtaggtcg tggtcgtttg
     4261 actgcccgtc aaatcgaagc tgctcgtcgt gcaatgaccc gtcatatcaa acgtggtggt
     4321 cgtatttgga ttcgtgtatt ccctgataaa ccgattactg aaaagcctat tcaagttcgt
     4381 atgggtggcg gtaaaggtaa cgtggaatat tacattgccg aaattaaacc aggtaaagtg
     4441 ttgtatgaaa tggatggcgt tccagaggaa ctggctcgtg aagcattcga gttggctgct
     4501 gccaaattgc ctattcctac aacctttgta gtaagacagg tgggtcaata atgaaagcaa
     4561 atgaattgaa agacaaatcc gttgagcagt tgaatgcaga tttgttggac ttgttgaaag
     4621 ctcagtttgg cttacgtatg caaaacgcta ccggtcaatt aggcaaacca agtgaattga
     4681 aacgtgtacg tcgcgatatt gctcgtatta aaaccgtttt aactgaaaaa ggtgctaagt
     4741 aatgagcgaa actaaaaatg ttcgtacttt gcaaggcaaa gtagtaagcg acaaaatgga
     4801 taaaaccgta acagtattgg ttgagcgtaa agtaaaacat ccgctgtatg gtaagattat
     4861 tcggttatct actaaaatcc atgcccatga tgaaaataat caatatggaa ttggtgatgt
     4921 ggttgttata tcggaatccc gtccattgtc aaaaactaaa tcttgggttg tcagtgagct
     4981 ggttgagaaa gcacgttcta tttaagaatt aaagcaacgt gcttggaatg ggaaacgaag
     5041 tattgcagca aatttaattt gcgtgtaaac ttcgtttcct gtctttcagt ttcttctgga
     5101 agtttcttcc ctttcggggt ccaagactgg tttacttgaa ccgcaaggtt tcatttaata
     5161 agcagcggct ttgctgtaag ttatctgaaa gtggtaaatt aagttggtta atttaaaggt
     5221 aataacatga ttcaaatgca gaccatctta gatgtggctg ataactctgg tgcgcgtcgc
     5281 gtaatgtgta tcaaggtatt gggcggatct aagcgtcgct acgcttctgt tggcgatatt
     5341 attaaagtgg cagttaaaga tgcggctccg cgtggccgtg tcaaaaaagg cgatgtatat
     5401 aatgcggtag ttgttcgtac tgctaagggt gtacgtcgtc ctgatggtgc gttaattaaa
     5461 ttcgataaca atgccgccgt gttactgaat aataaacttg aacctttggg tactcgtatc
     5521 tttggtccgg taacccgtga attgcgtact gagcgattta tgaaaatcgt ttcattggca
     5581 cctgaagtat tataaggaat ggcacgatga ataaaatcat taaaggcgat agggttgtag
     5641 taattgctgg taaggataaa ggtaagcagg gtcaagtagt tcgagtgttg ggtgataaag
     5701 ttgttgttga gggcgttaat gttgtaaaac gccatcaaaa acctaatcca atgcgtggca
     5761 ttgagggcgg tattattact aaagaaatgc ctttggatat ttctaatatc gcaatcctga
     5821 atccggaaac taataaagcg gaccgtgttg gtattaagct gattgaaaat gaaggcaaag
     5881 ttaaacgcgt tcgtttcttc aaatcaaatg gctctatcat tggggcataa ggagataaca
     5941 tggctcggtt gagagagttt tataaagaga cagttgttcc tgaattggtt aaacaatttg
     6001 gttacaaatc agtaatggaa gtcccgcgta ttgaaaaaat caccttgaat atgggtgtgg
     6061 gtgaggctgt tgctgacaaa aaagttatgg aacatgctgt ttccgattta gagaaaattg
     6121 ccggtcaaaa accggttgtt actgttgccc gtaaatctat cgcaggtttt aaaatccgtg
     6181 ataactatcc ggttggttgc aaagtaacat tgcgtcgtga tcaaatgttt gaattcttgg
     6241 atcgtttgat tactattgca ttacctcgcg tacgtgactt ccgtggtgtg agcggtaaat
     6301 catttgatgg ccgtggcaat tacaatatgg gtgttcgtga gcaaattatt tttccggaaa
     6361 ttgaatacga taaaattgat gctttgcgtg gtttgaatat tactattact actacagcaa
     6421 aaaccgatga ggaagcgaaa gctttattgt cattgtttaa atttccgttc aaaggataat
     6481 catggctaag aaagcactta ttaatcgtaa tctgaaacgt caagctttgg ctaaaaaata
     6541 tgcggctaaa cgcgcggcaa ttaaagcggt aatcaatgat tcgaatgcaa ctgaggaaga
     6601 gcgttttgag gctcgtttga ggtttcaatc cattcctcgt aatgcggcac ctgtgcgtca
     6661 acgtcgtcgt tgtgctttga caggtcgccc tcgtggtact ttccgtaaat ttggtttggg
     6721 tcgtattaaa atccgtgaaa tcgccatgcg tggcgaaatt ccgggtgttg ttaaagccag
     6781 ctggtaatag gagtaattaa gaatgagtat gcatgatcct atttccgata tgttgactcg
     6841 tatccgcaat gcgcaacgtg ctaataaagc agcggttgca atgccttctt caaaattaaa
     6901 gtgtgctatt gcaaaggtat tgaaagaaga aggatatatt gaggacttcg cagtttcatc
     6961 tgacgtaaag tctatattgg aaattcaatt aaaatactat gcaggtcgtc ctgtaattga
     7021 acaaatcaag cgtgtatctc gccccggttt gcgtatttat aaagcgtcta gtgagattcc
     7081 aagtgttatg aatggcttgg gtattgctat tgttagtact tctaaaggtg taatgactga
     7141 tcgtaaagca cgttctcaag gtgttggtgg tgagttgtta tgcattgtag cctagtggag
     7201 gaaaagaaat gtcacgtgtc gcaaaaaacc cagtgactgt tcccgctggt gtagaagtaa
     7261 aatttggaac agaggcatta gttattaagg gtaagaacgg tgaattgtct tttcctttgc
     7321 attctgatgt agccattgaa tttaatgatg gcaaattggc ttttgttgcg aataacagca
     7381 gtaaacaagc aaatgcaatg tctggtactg ctcgcgcatt agtcagcaat atggttaaag
     7441 gtgtttcaga aggttttgag aaaagattgc aattgatagg tgtgggttat cgtgctcaag
     7501 cacaaggtaa aatcttgaat ctgtctttgg gtttttctca tccgatcgta tatgaaatgc
     7561 ctgaaggtgt ctccgttcaa actcctagcc aaacagagat tgttttaacc ggctcggata
     7621 aacaagttgt tggtcaagtt gctgctgaga ttcgtgcgtt ccgtgctcct gagccttata
     7681 aaggtaaagg tgttcgctat gtaggagaag tagtggtaat gaaagaagcc aagaaaaaat
     7741 aattgaggtt cactaatgga taaacataca acccgactcc gtcgtgcacg caaaacccgt
     7801 gctcgtattg cggacttgaa aatggtaaga ttatgtgtgt tccgaagcaa taatcatatt
     7861 tatgctcaag taattagtgc tgaaggtgat aaagtattgg ctcaagcctc tacattggaa
     7921 gctgaggtgc gcggtagtct gaaatctgga agcaatgttg aagcagctgc aatagttggt
     7981 aaacgtatcg ctgagaaggc taaagcagca ggtgtagaaa aggttgcttt tgatcgttca
     8041 ggtttccaat atcacggtcg tgtgaaggct ttggctgaag ctgctcgtga aaatggttta
     8101 agcttctaaa tatttggaga ctttcagatg gcaaaacatg aaattgaaga acgcggtgac
     8161 ggtctgattg aaaagatggt cgcagttaac cgtgtaacca aagtagttaa aggtggtcgc
     8221 attatggctt tctctgcgct aactgttgtt ggtgatggag atggtcgtat tggtatgggt
     8281 aaaggtaagt caaaagaagt gcctgttgca gtccaaaagg cgatggatca agcacgacgc
     8341 tctatgatta aggtaccatt aaaaaatggt acgattcatc atgaggttat tggtcgacat
     8401 ggtgctacta aagtatttat gcagcctgct aaagagggta gtggcgtaaa agccggtgga
     8461 cctatgcgtt tggtttttga tgctatgggc attcataata tctccgccaa agtgcacgga
     8521 tctactaacc catataatat cgtacgtgca acattagatg gtttgtctaa gttgcatact
     8581 cctgctgata tcgcagccaa acgtggcttg acagtggaag acattttggg agttaaccat
     8641 ggctgaacaa aaaaagatta gggttacatt ggttaaaagc ctgattggta caattgaatc
     8701 tcatcgtgca tgtgcacgcg gtttaggttt gcgtcgtcgc gagcatacgg tagaggtttt
     8761 agatacccct gaaaaccgtg gtatgattaa taaaatcagc tacttgttga aagtggagtc
     8821 ttgatatgtt tttgaataca attcaacctg ctgttggtgc tacgcatgct ggtcgtcgtg
     8881 ttggacgcgg tattggtagt ggtcttggca aaacgggtgg tcgtggtcat aaaggtcaaa
     8941 agagccggtc tggtgggttt cataaggtgg gtttcgaggg tggtcaaatg cccttgcaac
     9001 gacgcctccc taaaagaggt tttaaatctt taacagcatc agctaatgca cagcttcgtt
     9061 taagtgaact ggaatcaatt gctgttaatg agattgatat tttggtctta aagcaagcgg
     9121 gtctgattgc atctacagtc tctaatgtta aagttattgc ttctggtgaa atttctaagg
     9181 cagttgcttt gaagggtatt aaagttacca aaggtgcgag agctgctatc gaggctgttg
     9241 gtggtaagat tgaaatgtaa ggtttaatat tgtggctaat caacaaacgt catcaggttc
     9301 atccaaattt ggagatctta agaaacgtct tttgtttcta tttggagcat tgattgtttt
     9361 tcgaattggt gcccatatac ccgtacctgg agttgatgct gttgctttag ctaaattata
     9421 cgaaagcgct ggaaacggca tcctgggaat attgaatatg ttttccggtg ggtcgttaga
     9481 gcgctttagt atatttgcaa taggaattat gccatatatt tcagcttcta ttattgtaca
     9541 gctcgcttct gaaattttgc catcattgaa ggctttaaaa aaagaagggg aggctggtag
     9601 aaaggtaatt acgaaatata ctaggtatgg tactgttttg ttagcaattc ttcaaagtct
     9661 aggtgttgca tctttcgtat ttcagcaagg aattgttgta acaagttcat ttgagtttca
     9721 tgtttccacg gtagtttctt tggtaacggg aaccatgttt cttatgtggc ttggggagca
     9781 aattactgaa aggggtatcg ggaacggtat ttctttaatc attacggcag gtattgcttc
     9841 aggtattcct tcgggtattg caaagctggt tacactgacg aaccaaggtt ctatgagcat
     9901 gcttacggcg ttgtttattg tatttggtgc cttattatta atttatttgg ttgtatactt
     9961 tgaaagtgca cagcggaaga ttcctattca ttatgcaaaa cgccagttta atggtagggc
    10021 gggtagtcaa aatacgcata tgcctttcaa gttgaatatg gctggtgtta ttcccccaat
    10081 ttttgcttcc agtattattc tatttccatc tactctttta ggttggtttg gttcggctga
    10141 tacaaatagt gttttgcaca aaatagctgg attgttacaa cacggtcaat tgctgtatat
    10201 ggctttattt gcagcgacag ttattttctt ttgttatttt tatacggctt tggtttttag
    10261 ccctaaagaa atggcagaga atttaaaaaa gagtggtgct tttgttcctg ggattagacc
    10321 tggtgagcag acctctaggt atttagaaaa agttgtatta cgtttgacat tgtttggagc
    10381 tctttatatt acaactattt gtttaattcc agagttctta actacggttt taaatgtacc
    10441 tttttatttg ggtggcacgt ctttgttgat tctagttgtt gtaacgatgg attttagtac
    10501 acaaataaat tcgtataggc ttactcaaca gtatgataag ttaatgactc gttcagaaat
    10561 gaaatcattt tctcggaaat agaattatgg cgaaagaaga tactatccaa atgcaaggtg
    10621 aaattcttga aactttacct aatgcaacat ttaaagtaaa acttgagaat gaccatattg
    10681 tattgggtca tatttctggg aagatgcgga tgcattacat tcgtatttct ccgggagata
    10741 aggtcacagt agagctgaca ccttatgatc taactagggc tcgaatcgtt ttcagagcaa
    10801 gataaaccaa taaaaggaaa ataaaatgcg tgtacaacca tctgttaaga aaatttgccg
    10861 aaattgcaag attattcgtc gaaatcgtgt agttcgtgta atttgtactg atctccgtca
    10921 caaacagcgt caaggttaat ggaatatttc ttttaatgtg attctgtgat atagtgacac
    10981 actttgccct aaaaaggaaa aaatatggct cgtattgcag gggtaaatat ccctaataac
    11041 gcacacatcg taattggtct tcaggctatt tacggtattg gtgctactcg tgctaaattg
    11101 atttgtgagg ctgcaaatat tgcgcctgat actaaagcaa aagatttgga cgagactcaa
    11161 ttagatgctt tgcgtgacca agttgccaag tatgaagtag aaggtgattt gcgtcgtgag
    11221 gtaactatga gtatcaagcg attgatggac atgggctgct atcgtggctt ccgtcatcgt
    11281 cgcggcttac catgccgcgg tcaacgcact cgtacaaatg cgcgtacccg caaaggtccg
    11341 cgtaaagcga ttgctggtaa gaaataaatt ttaaggaatt ttattaatgg ctaaagcaaa
    11401 cacagcttca cgtgtacgta aaaaagtacg taaaaccgtg agtgagggta ttgtgcacgt
    11461 tcatgcatct ttcaacaata ccatcattac aatcactgac cgtcaaggca atgcgttgtc
    11521 ttgggctacc tctggcggcg ctggttttaa aggttctcgt aaaagtacac catttgcagc
    11581 acaagttgca gcagaagcag ctggtaaagt tgcccaagag tatggcgtta aaaatttaga
    11641 ggttcgtatt aaaggtccag gtccaggtcg tgaatcctct gtacgtgctt tgaatgctct
    11701 tggtttcaag attaccagca ttactgacgt taccccgttg cctcataacg gttgccgtcc
    11761 gcctaaaaaa cgtcgtattt aatattggag tgatttgaaa catggcacgt tatattggcc
    11821 ctaaatgtaa gttggcacgt cgcgaaggta cggatttgtt tttgaagagt gcgcgccgct
    11881 ctttggattc taaatgtaaa attgattccg ctcccggtca gcatggtgca aaaaaaccgc
    11941 gtttgtcaga ctatggtttg cagttgcgtg aaaaacaaaa aatccgccgt atttatggcg
    12001 tattagaacg tcagttccgt cgttatttcg cagaagctga tcgtcgtaaa ggttctaccg
    12061 gcgagttgct gttgcagttg ctggaatctc gtttggataa tgtcgtttat cgtatgggtt
    12121 tcggttctac ccgagctgaa gcaagacagc ttgtttctca taaggcgata gttgtgaatg
    12181 gacaagttgt caatattcct tctttccaag tgaaagctgg tgatgttgtc tcagttcgtg
    12241 aaaaagccaa aaaacaggta cgtattcaag aagcattggg tttggcaact caaatcggct
    12301 tgccgggttg ggtttctgta gatgcggata aacttgaggg tgtgttcaaa aacatgccgg
    12361 atcgctcgga attgaccggt gatattaatg aacagctggt ggtagagttc tactctaaat
    12421 aatgctagct cagtgaggga cagttaaatg cagaatagca caaccgaatt tttgaaacct
    12481 cgtcaaattg atgtaaatac tttttctgca actcgtgcaa aagtatctat gcagccattt
    12541 gaacgtggtt tcggtcatac cttaggtaat gctttgcgcc gtatcttact gtcatccatg
    12601 aatggttttg ctcctactga agtagctatt gccggtgtat tacacgaata ttctactgtt
    12661 gatggtattc aggaagatgt tgttgacatt ttgctgaata ttaaaggtat tgtgtttaaa
    12721 ctccatggtc gtagccaagt tcaacttgtg ttgaagaaat caggttcagg tgtcgtatct
    12781 gccggtgata ttgagttgcc gcatgatgta gaaattctga atcctggtca tgtcatttgt
    12841 catttggctg ataacggtca aattgagatg gaaattaaag tagagcaagg tcgtggttat
    12901 caatctgttt caggtcgtca ggtagttcgt gatgagaacc gtcagattgg tgcaatccag
    12961 ttggatgcga gcttttcgcc catcagccgt gttagctttg aggttgaacc tgcacgtgta
    13021 gagcagcgga cggatcttga taagttggtt ttggatatcg aaaccgacgg ttctattgat
    13081 cctgaggaag ctgtacgcag tgcggcacgt attttgattg atcagatgtc tatttttgct
    13141 gatttgcagg gtacgcctgt ggaggaggtt gaagaaaaag cacctcctat cgaccctgtt
    13201 cttttgcgtc cggtggatga tctggaattg acagtacgtt cagctaattg tttgaaagct
    13261 gaggatattt attatattgg cgatttgatt caacgcactg aaaccgagct tcttaaaacg
    13321 ccgaatttgg gacgtaaatc tttgaatgag attaaggaag tattggcatc taaaggtttg
    13381 acactgggtt ctaagttgga agcatggcca cctgtaggct tggaaaagcc ttaatgaaga
    13441 attaaaggat aattgatatg cgtcatcgta atggcaatcg caaattaaac cgtaccagca
    13501 gtcatcgtgc tgcaatgctg cgtaatatgg cgaattcatt attgactcac gaaactattg
    13561 taacaactct gcctaaggcc aaggaattgc gccgtgtagt agagccgttg attacattgg
    13621 gtaaaaagcc gtcattggca aaccgccgtt tggcatttga ccgtactcgc gaccgtgatg
    13681 ttgtagtaaa actgtttggc gatttgggtc ctcgttttac tgctcgtaac ggtggttatg
    13741 ttcgggtgtt gaaatacgga ttccgtaaag gtgataatgc acctctggca ctggttgaat
    13801 tggttgacaa accggctgct gagtaatttt agtcatataa cgccatctgc cgaaaagcag
    13861 gtggcgttat ttttgcaata tctgataggt aatagggtat tggctatcat gtttaaaata
    13921 ttaattgaat agctaaggtt tgcgcggtaa acttacatca ttaaaaaatt ctatgatggt
    13981 ttatataatg aatgctttcg atataaagtc gacaaagatg gacgtattgt ctatatcttt
    14041 gcatacgtca gacttgtttg atttggaaga tgtgctggtc aaattgggca agaagtttca
    14101 agagtctggt gttgttccat ttgtgctgga tgttcaagag tttgattatc ccgagtcttt
    14161 ggatcttgct gcattggttt cgttgttttc aaggcatggt atgcaaattt tgggtctgaa
    14221 gcattctaat gaacgttggg ctgctgcggc tatgaagtat catttgctgt tttgtctgtc
    14281 tcattcggaa aatgttaaag aactgggtca ggttgaggtg cagaacacgg aggatggtca
    14341 gaaagcaagg aaaacagtat tgattacatc ccctgtccgt accggtcagc aggtttatgc
    14401 cgaagatggc gatttgattg ttacgggggc ggtcagtcag ggggcggaat tgattgcgga
    14461 tggcaatata catatttatg cgccgatgag ggggcgtgct ttggccggcg ccaagggtga
    14521 tacttctgcc cgcatattta tccactccat gcaggcagaa ctggtttctg tggcgggtat
    14581 ttaccgtaat tttgaacagg atttgccgga tcatctgcac aagcagccgg tacagatatt
    14641 gttgcaggat aatcggttgg ttatcagtgc aattggctca gagtaattgt ttgatatttt
    14701 aaaaaggaaa tattgtggca aaaattattg tagtaacttc aggtaagggc ggtgtcggta
    14761 aaacgactac cagtgccagt attgcgacag gtttggcatt acgcggatat aaaactgcgg
    14821 taattgattt tgatgtgggt ttgcgtaacc tcgacctcat tatgggttgc gagcgtcgtg
    14881 tcgtttatga cctgatcaat gtcattcagg gggaggcgac gctcaaccag gctttgatta
    14941 aagataaaaa ttgtgaaaac ctgtttattt tgccggcttc ccagactcgg gataaagacg
    15001 ctttgacacg cgagggcgta gaaaaagtga tgcaggagct gtccggcaag aaaatgggct
    15061 ttgagtatat tatttgcgac tctcctgccg gtattgagca gggtgcattg atggcgttgt
    15121 attttgctga tgaagccatt gtaacgacca atcctgaggt ttccagtgtg cgtgactccg
    15181 acaggatttt gggaattttg caaagcaaat cccataaggc agagcaaggc ggttcggtta
    15241 aagaacatct gttgattacg cgttattctc ccgaacgtgt ggcaaaaggc gaaatgctgt
    15301 ctgtacagga tatttgcgat attctgcata ttcctttgct gggtgtgatt cctgaatccc
    15361 aaaacgtctt gcaggcatcc aattccggag aaccggtcat ccatcaggac agcgtggcgg
    15421 cttccgaggc atataaggac gttattgccc gtcttttggg cgagaaccgt gaaatgcgtt
    15481 tcttggaagc tgagaaaaaa agcttcttca aacgtctgtt tggaggataa ggtatgtcat
    15541 taatcgaatt tttattcggc agaaagcaga aaacggcaac cgttgcccgc gaccgccttc
    15601 aaatcatcat tgcccaagag cgcgcccaag aaggtcaggc tccggattac ctgccgactt
    15661 tacgtaaaga gttgatggaa gtcctgtcca aatatgtgaa tgtttcatta gacaatatcc
    15721 gtatttccca agaaaagcag gatggtatgg atgtgcttga gttgaacatt actttgccgg
    15781 aacagaaaaa ggtataggac atgaccttaa ccgaattgcg gtacatcgtc gcagtcgccc
    15841 aagaacgtca tttcggcagg gcggcgcggc gttgttttgt cagccagccc actttgtcta
    15901 ttgccattaa gaaattggaa gaagagcttg ccgtctcttt gtttgaccgg agcagcaacg
    15961 atattattac gaccgaggcg ggggaacgta tcgttgcaca ggcgcgtaag gtgttggaag
    16021 aggcagagct tatcaggcat ttggcaaatg aagaacaaaa cgagctggag ggtgcgttca
    16081 aactcgggct gatttttacg gttgcgccgt acctgctgcc gaaactgatt gtctcgttgc
    16141 gccgtactgc accgaaaatg cctttgatgt tggaagagaa ttacacgcat actttgaccg
    16201 agtcgctcaa acgcggggac gttgacgcga ttatcgttgc cgaaccgttt caagagccgg
    16261 gcattgttac cgaacccttg tatgacgaac cgtttttcgt gattgtcccg aaagggcatt
    16321 catttgagga actggatgcc gtttcgccac ggatgctggg tgaggagcag gttttgctgc
    16381 tgacggaagg caactgtatg cgggatcagg tactctcaag ctgttccgaa ttggcggcga
    16441 aacaacgtat acaggggttg accaatacat tgcagggcag ctcgatcaat acaatccgcc
    16501 atatggtcgc cagcggtttg gcaatcagcg tgttaccggc aaccgcgctg accgaaaacg
    16561 atcatatgct gttcagcatt attccgtttg aaggtacgcc gccaagccgg cgggtcgtat
    16621 tggcgtaccg ccgcaatttt gtccgtccga aggcgttgtc ggcgatgaag gcggcgatta
    16681 tgcagtcgca gcttcacggg gtaagtttta tccgcgacta ggcgcaggca ttgttttcaa
    16741 aacgccattt ccctgagccg acaacacggt atgccaagat attgccgtca tcatcgattt
    16801 tgagtatagc atcgccacgg aaactgccgt cctgaagata ttcgactttt gcatcactgt
    16861 gaatgttttc atcagtgccg atgcaatgcc atgtatagtg gattaaca
//
LOCUS       DAWFBO010000027        16779 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFBO010000027 DAWFBO010000000
VERSION     DAWFBO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.027"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(177..1346)
                     /locus_tag="WGO87_000660"
     CDS             complement(177..1346)
                     /locus_tag="WGO87_000660"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0392051.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1520..1630)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1683..2579
                     /locus_tag="WGO87_000661"
     CDS             1683..2579
                     /locus_tag="WGO87_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0392052.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLANVKGVKTLFFCCERLPDGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2623..3687)
                     /gene="tsaD"
                     /locus_tag="WGO87_000662"
     CDS             complement(2623..3687)
                     /gene="tsaD"
                     /locus_tag="WGO87_000662"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0392053.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVK"
     gene            complement(3803..4990)
                     /gene="ccsB"
                     /locus_tag="WGO87_000663"
     CDS             complement(3803..4990)
                     /gene="ccsB"
                     /locus_tag="WGO87_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0392054.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLCAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4983..6935)
                     /locus_tag="WGO87_000664"
     CDS             complement(4983..6935)
                     /locus_tag="WGO87_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0392055.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVNIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7212..7835)
                     /locus_tag="WGO87_000665"
     CDS             complement(7212..7835)
                     /locus_tag="WGO87_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0392056.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            8041..8670
                     /locus_tag="WGO87_000666"
     CDS             8041..8670
                     /locus_tag="WGO87_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0392057.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8791..11187)
                     /locus_tag="WGO87_000667"
     CDS             complement(8791..11187)
                     /locus_tag="WGO87_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0392058.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPKVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11339..12454
                     /locus_tag="WGO87_000668"
     CDS             11339..12454
                     /locus_tag="WGO87_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0392059.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQSTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12457..13056
                     /locus_tag="WGO87_000669"
     CDS             12457..13056
                     /locus_tag="WGO87_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0392060.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKENKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13057..13704
                     /locus_tag="WGO87_000670"
     CDS             13057..13704
                     /locus_tag="WGO87_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0392061.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13722..14267
                     /locus_tag="WGO87_000671"
     CDS             13722..14267
                     /locus_tag="WGO87_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0392062.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14286..16547
                     /locus_tag="WGO87_000672"
     CDS             14286..16547
                     /locus_tag="WGO87_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0392063.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQTNIDFRKDG
                     KNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLK
                     RLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISL
                     DFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQ
                     QGNIVNIAPRDELLAKDKALLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNAD
                     TTGNRNTLISGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVE
                     AADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINLPITAAANS
                     ISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIAN
                     GGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLN
                     TQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFIT
                     PRIMGTAGNSLRY"
BASE COUNT         4499 a         4758 c         3825 g         3697 t
ORIGIN      
        1 ctcatggaaa atcccctaaa tgtcttggtg ggaatttagg ggattttggg gaattttgca
       61 aaggtctcta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      121 tatataaaaa tgccgtctga actgttcaga cggcattttt gattttcaaa ccggaattac
      181 agccccgctg ccgccctcaa tgcagcagct ttgtcggtgc gctcccaagt gaactcaggt
      241 tcttcgcggc cgaaatgtcc gtaagcggcg gatttactgt aaatcgggcg caagaggtcg
      301 agcatttgga cgatgccttt ggggcgcagg tcgaaatgtt cgcgaactaa ggtaatcagt
      361 ttttcttcgc tgattttgcc tgtgccgaaa gtatcgatgg aaatcgaagt cggttcggca
      421 acgccgatgg cgtaggaaac ttggatttgg cattgggttg ccaatcctgc ggcgacgatg
      481 tttttcgcca catagcggca ggcgtaggcg gcggaacggt caaccttgga cgggtctttg
      541 ccggaaaacg cgccgccgcc gtgcggagcc gcgccgccgt aggtatcgac gatgatttta
      601 cggccggtca aaccgcagtc gccttgcggg ccgccgatga cgaagcggcc ggtcgggttg
      661 atcaggtatt tggtttcgtc ggtcagcagt tcagacggca ggacgggctt gataatctgc
      721 tcgatgacgg cgtttttcag ttcttcgtaa gcgatggacg gatcgtgctg ggtagagagg
      781 acgacggtat caatgcgttt taccttaccg gtttcgctgt cgtaaaccac ggtcaactgc
      841 gcttttgcgt cgggacgcag ccacggcagg cggccgtctt tacgcaattc gctttggcgt
      901 tgcatcaggc ggtggctgta atagatggca aacggcatca gggtcggggt ttcgtcgcag
      961 gcataaccga acatcaaacc ttggtcgcct gcgccttggt tcaagtcgat accttcgcct
     1021 tcgtttacgc cttgggcgat gtcgggggat tgttggtcgt agtacacgcc gaccgcgcag
     1081 ccgttggcat caaagcccag ctcggaggag ttgtagccga tgcgtttgat ggtttcgcgt
     1141 gcgactttga tgtagtccac ttgggcggtg gtggtgattt cgcctgccaa tacgcacaag
     1201 cctgtgttga ccaaagtttc tgcggcgaca cgcgctttgg ggtcttgcgc caagatggca
     1261 tccaaaatcg catcggacac ttggtcggca actttgtccg gatggccttc ggataccgat
     1321 tcggaagtaa acagatattc gctcattgct attctttcat taaaaacagg ctgctgtgtt
     1381 ttcagacggc cttttatttc ggacagcgga aatcataaca aaaactccgc tttttctcta
     1441 tacaagtgag aaacattcgc acttgtctgc tgtttcggaa tcctgttgcg cgtttcttac
     1501 aggcaaaaaa attcccgcat taagcgggtt tggattgctc tggtgagcca catcggcttt
     1561 tcaaccgtcc accttacttt tccttttgaa aagcaggttg gcatggaatt cccaactctt
     1621 aatgcagcgc ggatggtagc agaaaagcct ttccgccgca atacttccct ttttcagacg
     1681 gcatgtttcg tttacaattc gggctgtttc cccctttgcg aaccgccatg cacatcctgt
     1741 tgaccgccct gctcaaatgc ctctccctgc tgccgctttc ctgtctgcac acgctgggaa
     1801 accggctcgg acatctggcg ttttaccttt taaaggaaga ccgcgcgcgc atcgtcgcca
     1861 atatgcgtca ggcaggcatg aatcccgacc ccaaaacggt caaagccgtt tttgcggaaa
     1921 cggcaaaagg cggtttggaa cttgcccccg cgtttttcag aaaaccggaa gacatagaaa
     1981 caatgttcaa agcggtacac ggctgggagc acgtgcagca ggctttggac aaacacgaag
     2041 ggctgctgtt catcacgccg cacatcggca gctacgattt gggcggacgc tacatcagcc
     2101 aacggctgcc attcccctta accgccatgt ataaaccgcc gaaaatcaaa gcgatagaca
     2161 aaatcatgca ggcgggcagg gttcgcggca aaggaaaaac cgcgcctacc agcatacaag
     2221 gggtcaaaca aatcatcaaa gccctgcgtt cgggcgaagc aaccatcgtc ctgcccgacc
     2281 acgtcccctc ccctcaagaa ggtggggaag gcgtatgggt ggatttcttc ggcaaacctg
     2341 cctataccat gacgctggcg gcaaaactgg caaacgtcaa aggcgtgaaa accctgtttt
     2401 tctgctgcga acgcctgcct gacgggcagg gtttcgattt gcacatccgc cccgtccaag
     2461 gggaattgaa cggcgacaaa gcccatgatg ccgccgtgtt caaccgcaat gccgaatatt
     2521 ggatacgccg ttttccgacg cagtatctgt ttatgtacaa ccgctacaaa atgccgtaac
     2581 gaaaataaaa atgccgtctg aacaatttca gacggcattt tgtcatttga cgatttccga
     2641 cagcggccag cgcggacgga cattgaacgc accgacctcc ctgcccttgc ccaggtgcat
     2701 cgcaccggca aaggcaatca tggcaccgtt gtccgtgcag tatgccgtcg gcgggaaaaa
     2761 cacgctgact ttttcggacg gatgtttcgg cttgcctttg ggggtcggga tttgcaccgt
     2821 catgttgccg aaagtttcac ggagcttgcg gtttgcaccg accccgccgg cgaccactac
     2881 ggttctgaac cctgtctgca acagggcttt tttcactttc gccgccaaca catcgactac
     2941 cgcatcttga aacgcacggc agatgtcgtt gcgtgtctgc tcaggaatgt catccgcccc
     3001 gttttccgcg cgcactttct cgacggcggt caatacggcg gttttcaaac ctgaaaaact
     3061 catctgcaaa tcgtcggaat gaatcattgg gcgcggaaaa acaaacgctt cgggcctgcc
     3121 cgattccgca agttccgaca gtttcgcacc gcccggatac ggcaagccca gcagtttcgc
     3181 cgttttgtcg aatgcctcgc ccgccgcatc atcgacgctc tcgcccaaaa gcgcgtagtc
     3241 gcctataccc ctgaccgcca taatctgcgt atgcccgccc gaaaccagca gcgcgacaaa
     3301 aggaaagtcg ggtttttcct ccgccaacag cggcgacagc agatgtcctt ccaaatgatg
     3361 gacggggatg acgggcttgc ccaatgccaa agccagtgca ttggcgtagc tcgaacccgc
     3421 cagcagcgca ccgcccaaac cgggtccctg cgtaaaggca accgcgtcaa tgtcgccata
     3481 cgatgcgccc gcctgcgcca aacagccttc cgtcaacgga acaaggcggc ggatatggtc
     3541 gcggcttgcc aattccggca caaccccgcc atattcggcg tgcattgcca tctgcgtgtg
     3601 cagacagtgc gcccgcaatc ctcgttccgt atcgtaaagc gcaacgccgg tttcgtcgca
     3661 agaagattcg attcctaata ccaacatggt ctgatgccgt taaaaactga aaaacgtatt
     3721 ctagcggatt tcggcacgac tgccgtatcc caaaaacgga acatgccgtc tgaagccctt
     3781 cagacggcat cgtcgcaccg tatcaaagcg ttccgtaaga atgcagcccg ctcaaaaaca
     3841 tattcacgcc gataaaggcg aatgcggtta cgaacaaacc gataatcgcc caccacgcca
     3901 gcactttgcc gcgccaaccg gcaaccagcc gcaagtgcag ccaaacggcg taattgagcc
     3961 agacgatgaa cgcccacgtc tctttcgggt cccaactcca atagcgtccc caagcatccg
     4021 ccgcccacag cgcacccaaa atggtggcaa tggtaaagaa cagaaagccg acggcaatcg
     4081 ccttatacat gacttcctca atcaacacgg attgcggcag ccacgatttc ccgccttttc
     4141 cttccgcaca cagggaaacc agttcggcaa taccgagcat cgcggaaatg caaaacgcgc
     4201 cgtaaccgat aaagtttgcc ggaacgtgga ttttcatcca ccaagattgc aatgccggaa
     4261 tcagcggctg gatggtatgc gcctcgcggg acacgctgta ccacaagaca aatccaacca
     4321 cgaccgccat aaagccgaac acgaagccgc ccaatttctg tatggcgaac ttaccttcat
     4381 aataaagata catcagcgcg gtaatcacca aaaacaggat gaacacttca tacaggttgg
     4441 aaaccggaat atgccccgca tcgggacgga gcagatagct ttcgtgccaa cgtaccagca
     4501 gaccggtaaa gcctgctacg gcagacaccc atgcaaacac ggttcccata cccaacagcg
     4561 tgttggtcgg cacatttttt acgcttgcca aaaccgcgcc cgaaatatag gcgaacaggg
     4621 cgaagaagac aaaggcgcac tgccacatga tcgccgactg gctgctgagg aaataccgca
     4681 acaggaaaat ctctgccgat ttgatgttgc cgccatacaa accgacggcg gcataggcaa
     4741 gcaatacgct taaaggaaca aaccagcgca tcggtttgaa aaaccaaccc aaaaacacgg
     4801 caataccggc acttgcccac aacatgaccg tttcgtaaat gtccatatgc ataccggaac
     4861 gggtctgtac gaaaaccgta gccgcaaaaa ccagcacggc aaatacccaa tcccaaagat
     4921 tcagattgct gatcaaagac ttctgaatca gcagctcgtg ttccggaagg gttttatagt
     4981 gttcagtcat gattcaagtc cttgccgagc cgttgcagac tctcgacgtg ttttggaaat
     5041 tccttctgca aatcccgttc gctgcgggcc gaagacatgg caaaacggat tttgccgtct
     5101 gaaaacaata cccacgcccg tttttcgcgc acataaaaca tcaataccgt acccaatacc
     5161 aacagcaccg agccgagata gaccaaaagc gcacccgggg aacgggtcat ctgcaaaccc
     5221 gacgaacgca cctcggaaaa cccatcaagt tgcagcagca taggcgcggg atattcggtc
     5281 aaacccgtgt acgcatccat actgtgcagc aggaaacgat tccgcgcttc atcctgctgc
     5341 cattcgggca agccgtaccg gcgtatggtt tcatccaaag cagcgttcat cacgccgtaa
     5401 agcatttcgt agaaatagcc ctgcatctta tcctgctgct ctttcgggat attggacgta
     5461 ataaattcgt ccaatcccaa atagcctttt tgtgcaaaga tgttcagcgt gttttccgca
     5521 gccagcatga attgttcgcg gatttcggca ggtgcgcctt tggttgcgtc ggcaaccaga
     5581 cgtttgcgcc cttccccatc tttcaaaaac tcacgcaatg ccataaaggt gtccgctttc
     5641 aactgcttgt ccaaggggat acgcagccag cggtattgct gctgcaagcc gctgcgcgtg
     5701 ccggtaatcc aaaaataatc ctgttcctgc aaaaccggca gcatatagtt tttatattcg
     5761 accgcctgcc ctgccgcatc acggatacgg taaacaatgg aagggccgat attggtgtat
     5821 tttttacctt cctgagtaac ggcgcggaca tcgttcagcg tggatttcag gcttttttcc
     5881 cgttccgcgc cctcgctcat gtcctccaca ttcatagaag taaactgatc gaactcaaga
     5941 cgatatttgt gtttgccaat ttccaacgga aactggtgta tggatgttgc cttcaacacg
     6001 acaggctcgc gcgaagcatc acccaaattc cacgccttga atgtcaaatc cgaaccgccg
     6061 tcggcaaaac tcgcctgata aatcgtgatg ccgtgcaagg tcaaaggatg gttcacgcgg
     6121 atggtgcgct cgagtttctc accggttgcc ttatccgtta cttcaatatc actggcaaaa
     6181 tcgcgcggca tacccgtatt gtaaaaatcg atatggaatt ttttcagttt gacttcaaaa
     6241 ggcaagtcct gaaccaatat cccgttgtcg gcattcagga aaaccacatc cgcactctgc
     6301 ccctcggaaa tattgacgtt gcccctaaat gagagattgg acgcacccaa aatactttcg
     6361 ggcttgaaat ccttggcata aaccgcctga ttgtccggaa caatccgacc ggtcagcata
     6421 cccagtttca acagcaggtt actgtctatc aacccgccca ggcaaatgac aatcaaagca
     6481 acatgggcaa agatatagcc ccatttgttc attgtgcctt ttttggcggc aatcagaacc
     6541 gacccgtctt cacggttaat ggtttttccc tgaaaacctt gtacttccag ataacgtttg
     6601 gcaacctcgg gcgcaatgtt tacatccaac agcgaagaat ggcgcatcgc cgccagagat
     6661 ttttctttaa ccttttcccg aaaagacttc atttcgcgcc agaacggcgg cacattgcga
     6721 atcaggcaca aactggtaga aaccaccaaa aacatcatga taacgacaaa ccatgccgaa
     6781 gcatagacgt catacagtcc cagaaaacca aaaatctgcg cccaaaacga tccgaatttg
     6841 accaaataat ccgtctgcgg ctggttttgc tgcaacaccg taccgataac cgatgcaata
     6901 cccagcagac tgagcaaagc gactgcaaag cgcatggagc tgaaaaaagc gaaccacgga
     6961 cgggaaagaa gtgggggaga tctacgggat ttactcattg tgtgtttatt ccgccatcag
     7021 gaatatggga aagcagaatt gggcaaacag aaaacaacgt cccgattcta ctgtcttgat
     7081 gctttttatt tcaagacaat gaagacagcc tgcatcgatt ccaacggttg cgattgaaaa
     7141 aacttatcgc agaattgcct gaagccgtct gaaaactttt cagacggcct ctaaaacaga
     7201 ctattgcgga attaacgcaa accttggata aagttggcga ccgctttcaa atcttcttca
     7261 gacatacggt ttgcaatatc ttccatgatg gtatttttac gctgaccgga cttgtaggca
     7321 ttcatctgtt caacaatata tgcctgatgc tgaccgccca aacgcggata agcctgaatt
     7381 tcgcttccgc ctcccggcat acccgcaccg ctcggaccgt ggcaggacat acacgccggc
     7441 acttttttat cgctcaaacc gccgcgatag attttcgcac ccaattcggg attttcctta
     7501 ggattggctt caccggattt gggctgctgt ttggcataga atgcggatac gttcaaaata
     7561 tcctgatcgc tcaaattcat taccaccggt ttcatcacag ctgccgaacc gtgggtgcgt
     7621 ttaccgtcgc ggatgccgat agtttgatga tagatgtaag cagtatgctg tgccgccaaa
     7681 cgcggataca tcgcaatgcc gctgttaccg tctgctgcat ggcaagccgc acaaaccgtt
     7741 gcggcaacct gtttgccttt ttccacgtct gctttgggag aggcggaaac cgcaccggca
     7801 gccaaaacaa aggccaataa agtcaatcgt ttcatggagt gctcctgatt acagcattgg
     7861 ataacgcaac aatgctcttt ttatattcaa atacgggatt tttgacccga ttaaaaccga
     7921 tgattctgta aacgtgttat tctatactaa atttacatta aattaccact gtgtttcaca
     7981 taaaaccaac cgcatatttt tgctgtcgga caaacggcgg cggaaaacaa ggatatgccc
     8041 atgaaccttt ttcaaaacgc caaattcttc acgacggtca accaccttaa agacctgccc
     8101 gacacccctc tcgaaattgc ctttgtcggc aggagcaatg ccggaaaatc cagtgccatc
     8161 aataccctga ccaaccatgt ccgtcttgcc tacgtttcaa aaacacccgg acggacgcag
     8221 catatcaact tcttcgagct gcagaacggc aattttatgg tcgatttgcc cggctacggt
     8281 tatgcccaag tccctgaagc agtacgcgca cattgggtca atctgctcgg cgactatctg
     8341 caacagcgca aacagcttat cgggctggtt ttgattatgg atgcccgcca tcctttaaaa
     8401 gaactcgaca tccgtatgct ggattttttc cacacgaccg gcagaccggt tcacatcctg
     8461 ctgtcaaaag ccgacaaatt atccaaaaac gaacagataa aaaccctgtc ccaagtcaaa
     8521 aaactgctca aaccttattc cgacaggcaa aacatcagcg tacagctgtt ttccagcctg
     8581 aaaaaacaag gtattgacga ggccaaccga actgtcggaa gctggtttga cgcagcagat
     8641 gccgccgctt cctctccaga ggaaaactga ccccaattat acggaaaccg tatttccccc
     8701 acttgaccga ccgcaaacat ttaaaaaatt gccactgcca aatctaaaat gccgtctgaa
     8761 agacttttca gacggcattt tgcggagtct ttaaaacaga gaatccaact gctgctgttt
     8821 ggaaccagta ttactcggaa gcaccggcgt ttcctgcata tcttggcgga cttcgtcatc
     8881 cgccgcctgc cgtccgcctt ctgccgcgcc cccgtcatct tcttttgccc gtcgggaagg
     8941 ttgcggcgca ataccgctgt tgtccagcgt caagcccgga tcggttacca tacgttcctt
     9001 catatagtat tcgccattgc tgctgaccac accttcaggc attttcatcc ccttgccctg
     9061 ctttcctttc aacgcaaaac gcatatagtc cacccaaacc ggcaccgcaa tcgtaccgcc
     9121 gtagccgaca cgccccatac tcttaggttt gtcgaagccg atatatacgg cagtaaccac
     9181 atcagggtta aaaccgacaa accacgcatc cttattgtca ttggtcgtac ccgttttacc
     9241 ggcaatatcc gttcttccca acgcagctgc cccccttgcc gtaccaacac ggaccacatc
     9301 ctgcataatc ttatacataa tataggcatt gcgcggatcg attgcctgag gcgcattttg
     9361 cccggccacc aaaggttgca tttgggcgcg caacctgccg tctctgtcat aaatcttatc
     9421 gattacgtgc gaagaaaccc tatatccgcc gttcgcaaat acgctatatg cctccgccac
     9481 tttcaacggc gttgtctcgc ccgtacctaa agccatagac aggcttgccg gcagctcgga
     9541 cgacctgaag ccgaaacgcc ggatatactg ttgcgcgtaa ccgacaccga tagacatcaa
     9601 aatacggatg gaaaccatat tcttggaagc cgtcagagcc tgtctcaaag taatgtagcc
     9661 ggaatatctg ccgtctgaat ttttaggtgt ccaaaccgaa ccgttcggcc ctttccccgg
     9721 cagggaaatc ggcgcatcgt taaccactgt ggacgcggtc atccccttag ataatgccgc
     9781 cgaatagaca aacggcttaa aggtcgaacc cggctgccgc attgcctgaa cggcacgatt
     9841 gaatgttttg ctgtgaaaat cataaccgcc gaccagcgcg cgcacagctc cggtttttgc
     9901 atccagcgaa accaaagccc cctgcagcaa cggctcttga accaccgccc aacgcccgcc
     9961 gttgtttttg acacggatga ccgcgcccct gcggatacgg tcctccccca ttttttcatt
    10021 attgaccgcg cgggccgcaa aacccaaggc gcgcctgtca agcgtaaccc gcctgccgcc
    10081 gggcagctgt atgacgacat ttttcttttt agtcacatcc aacacaacgg cgggaaccat
    10141 tttatcgacg gtatagagtc ccgacagata ctggctgaca gtctcctcga catcttcact
    10201 cttactcaaa tcgatatagt tttccgcacc gcggtagctg ctgccgcgat cgaaattccg
    10261 tagagccttg cgcaatgcct cggttgccac cttctgatga tcagcgcgga ccgtggtata
    10321 aaccttaaaa ccctgcgtat aggcatcttc accgtatttc tcatacagtt cctgacgcac
    10381 catttccgcc acatataaag cactctgatc gattttccga acaaaccgct cgtaatgcag
    10441 ttcctcattc aacgcctgat cgcgctgttg cacggtaatc atcttctcct cgagcatatt
    10501 gttcaaaata tacttctggc gcaacttggc acgttctgga ttaacaatcg gattataggc
    10561 agacggagcc ttgggcagtc ccgcaagcat ggcggcttcc gccaaagtca aatctcggac
    10621 attcttattg aaatagattt gcgcggcaga tgcaaaacca taggcgcgct gaccgaggta
    10681 aatctgattg aaatacaact cgaggatttt gtctttgctt aaagactgct cgattttata
    10741 ggcaagcaac acctcattga atttgcgtgt gaacgttttt tcactgctca aataaaaatt
    10801 tttcgccacc tgctgcgtaa tcgtactcgc acccgactgc acgctgccgg acacgacatt
    10861 gccgacggca gcgcgggcaa caccccaaac atccaccccc caatgccggt aaaagcgttt
    10921 atcctcggcg gcgataaccg cattccgcaa cacctttggg aaatcgccga tttttgtaaa
    10981 ttcgcgccgc tgctccccat acataccgat gacttcccca tccgccgaat aaatagtcaa
    11041 cggcatttta ggctggtaat gctgcaaaga atccaaagac ggtagtttcg gatacgttac
    11101 caaaatagca atggcaacca aacccactcc aaatacacaa aacccaaaaa ccaaaccaaa
    11161 acaagtcgtt aaaatctttt taatcatagc tgaataataa tttaccatta ttggtattaa
    11221 ataaagtaaa atagcaaccg atttctacaa agcacggttt caatgtgcaa agaacaagga
    11281 atccattacg gataccgaaa cggttactca ctgtacaaat aaagcaggaa ctttcatcat
    11341 gcgcttgttt aaaagcttga aaaaccctaa aaaaacagat gccaagctcc ctaaaaaatc
    11401 ttcgggactc aataaccgcg cggcaatcgg catcgatatc gaccagcatt ccatcaaaat
    11461 ggtccaattg tcaggacgta gtttaaacca aattcaattg gaaaaatacg tcattgccaa
    11521 attaccaaag aatatcattc aaggcaataa agtccaaaat tacgatcaac ttgttacata
    11581 tttgcaacaa gcctatgcca aactgggtac ttcgtgcaaa aacatcatcg cgtccgtccc
    11641 gcaaaatctg gcaaccatcg aacaattgac ctacacagac aaagatgcag aattagacct
    11701 gcaggggttc gtggagtcct ccatctccga agtcagctcg atatcgctcg aagaagccaa
    11761 ttacgactat caggtcttgt cccaatcggc cgccggcgaa gctgtgttgg ccgtcgcatc
    11821 gagaaaggat gaaatcgaac ccctgattga cgcattcaac gccgccggta tgaaattatc
    11881 cgcgcttgat gtggacattt tcggacaata caacgcctac gcgctatgga taaaccattt
    11941 cgcccccgag cttgcagccg aaaaagtcgc cattttcggc gtatatgccg cacagaccta
    12001 cgccttggtc atccaagacg gaaaaatcct atacaaacag gaaacctccg tcagcgaaga
    12061 acagctcaac caactcatcc agagcaccta tcaggtaaca gaagaaaaag cggaagaaat
    12121 catcaactcc ccgcaaaaac cttccgatta ccaagaaagc gtggcaaact atttcaacca
    12181 gcagattacc caagaaatac aaagggtctt gcagttttat tacaccacgc agaccgcaga
    12241 cgatatgacc gacatcaagc atatcctgct gaccggggaa gcggcgcgcc aggaaggtat
    12301 cgcccaaacc gtcgcctcac aaaccaatgc agatgtacaa tgcgtccatc ccgcgcgtta
    12361 ttttgcggac aacctcaaaa cagacaaaca acaattcgaa cttgatgcgc cgacactgac
    12421 cagggcgttc ggtttggcgg tacggggatt ataattatga acaatttaat caaaatcaac
    12481 ctcctcccct acagggaaga gatgaacaag cgcaaacagc agcagtttaa aacgctgatg
    12541 tacggtgccg tgctgacggg cgttgccgcc gttgcggcaa cctacctgtt tatcgacaat
    12601 atgatcaata accagtcgga aagaaacacg ctgctggaaa cctccatcgc acacttggat
    12661 accgagctgt cggaaataca aaagctcaaa caggaaaaag atgccttcct gattaaggaa
    12721 aacaaaatcg aggagctcca gctcaaacgc ctccaagccg caaaaatcct cgacagcctg
    12781 aatgaggccg tccccggaag cacctacctg acctcgctgg atgccgttac cgccgactct
    12841 tatcggctca gcggcaggac atccagcgac aaccgcgttg ccgccatgat gagggcgatg
    12901 cccaataccg gcatattcaa gcaacccgaa ttgttaagca tcaagaaaaa caattcgcat
    12961 caagaattta cccttcaggc aacattacaa cccatcgtaa aggcggccga atccaaagag
    13021 aatccggctt cgggaaacgc acaggaggca aactgaatgg cttctaaatc atctaaaacc
    13081 aacttggatc tcaacaacct tcacctgctc aaccttcctg ccaggctttt tatcgccctg
    13141 ctggccgttg ccgccgtgct ggggctcggt tatgccggat tgttcaaaag ccagatggaa
    13201 tcccttgagg aatacgaagc aaaagaaacc gaactgaaaa acacctacaa acagaaaagt
    13261 atcgacgcgg ccagcctgaa caacctgagg gacgaacttg cctcaatccg ctctgccttc
    13321 gatatcatgt tgaaacagct gccgacagat gcagaaattc ccaatctggt tcaagagctt
    13381 catcaggcag gttcgagcaa cggcctgcgc ttggacagcg ttatgcccca acctcccgta
    13441 gatgacggcc ccatcaaaag attaccctat tccatttcca ttaccggaaa ttacgaacag
    13501 atcagccaat ttacccgcga tgtcggcagc ctctcccgaa tcattaccct tgagtcgctg
    13561 aaaatcgccc aatctccgga aaacggcggc aatcctgacg gcaagagcag catcctgaac
    13621 ctcagcgcca ttgccaccac ctaccaagca aaatccgtag aagagcttgc cgcagaagcg
    13681 gcacaaaatg ccgagcaaaa ataacttacg ttagggaaac catgaaacac tatgccttac
    13741 tcatcagctt tttggctctc tccgcgtgtt cccaaggttc tgaggaccta aacgaatgga
    13801 tggcacaaac gcgacgcgaa gccaaagcag aaatcatacc tttccaagca cctaccctgc
    13861 cggttgcgcc ggtatacagc ccgccgcagc ttacagggcc gaacgcattc gacttccgcc
    13921 gcatggaaac cgacaaaaaa ggggaaaatg cccccgacac caagcgtatt aaagaaacgc
    13981 tggaaaaatt cagtttggaa aatatgcgtt atgtcggcat tttgaagtct ggacagaaag
    14041 tctccggctt catcgaggct gaaggttatg tctacactgt cggtgtcggc aactatttgg
    14101 gacaaaacta cggtagaatc gaaagcatta ccgacgacag catcgtcctg aacgagctaa
    14161 tagaagacag cacgggcaac tgggtttccc gtaaagcaga actgctgttg aattcttccg
    14221 acaaaaacac cgaacaagcg gcagcacctg ccgcagaaca aaattaagaa gaggattact
    14281 ccattatgaa taccaaactg acaaaaatca tttccggtct ctttgtcgca accgccgcct
    14341 ttcagacagc atcggcagga aacattacag acatcaaagt ttcctccctg cccaacaaac
    14401 agaaaatcgt caaagtcagc tttgacaaag agattgtcaa cccgaccggc ttcgtaacct
    14461 cctcaccggc ccgcatcgcc ttggactttg aacaaaccgg catttccatg gatcaacagg
    14521 tactcgaata tgccgatcct ctgttgagca aaatcagtgc cgcacaaaac agcagccgtg
    14581 cgcgtctggt tctgaatctg aacaaaccgg gccaatacaa taccgaagta cgcgggaaca
    14641 aagtttggat attcattaac gaatcggacg ataccgtgtc cgcccccgca cgccccgccg
    14701 taaaagccgc gcctgccgca ccggcaaaac aacaggctgc cgcaccgtct accaagtccg
    14761 cagtatccgt atccgaaccc tttaccccgg caaaacaaca ggctgccgca ccgtttaccg
    14821 agtccgtagt atccgtatcc gcaccgttca gcccggcaaa acaacaggcg gcggcatcag
    14881 caaaacaaca ggcggcagca ccagcaaaac aaaccaatat cgatttccgc aaagacggca
    14941 aaaatgccgg cattatcgaa ttggctgcat tgggctttgc cgggcagccc gacatcagcc
    15001 aacagcacga ccacatcatc gttacgctga aaaaccatac cctgccgacc acgctccaac
    15061 gcagtttgga tgtggcagac tttaaaacac cggttcaaaa ggttacgctg aaacgcctca
    15121 ataacgacac ccagctgatt atcacaacag ccggcaactg ggaactcgtc aacaaatccg
    15181 ccgcgcccgg atactttacc ttccaagtcc tgccgaaaaa acaaaacctc gagtcaggcg
    15241 gcgtgaacaa tgcgcccaaa accttcacag gccggaaaat ctcccttgac ttccaagatg
    15301 tcgaaatccg caccatcctg cagattttgg caaaagaatc cggaatgaac attgttgcca
    15361 gcgactccgt caacggcaaa atgaccctct ccctcaagga tgtgccttgg gatcaggctt
    15421 tggatttggt tatgcaggcg cgcaacctcg atatgcgcca gcaagggaat atcgtcaaca
    15481 tcgcgccccg cgacgagctg cttgccaaag acaaagccct cttacaggca gaaaaagaca
    15541 ttgccgattt gggtgcgctg tattcccaaa acttccagtt gaaatacaaa aatgtggaag
    15601 aattccgcag catcctgcgt ttggacaatg ccgacacgac cggaaaccgc aacacgctta
    15661 tcagcggcag gggcagcgtg ctgatcgatc ccgccaccaa caccctgatt gttaccgaca
    15721 cccgcagcgt catcgaaaaa ttccgcaaac tgattgacga attggacgta cccgcgcaac
    15781 aagtgatgat tgaggcgcgt atcgtcgaag cggcagacgg cttctcgcgc gatttgggcg
    15841 ttaaattcgg cgcgacaggc aagaaaaagc tgaaaaatga tacaagcgca ttcggctggg
    15901 gggtaaactc cggcttcggc ggcgacgata aatggggggc cgaaaccaaa atcaacctgc
    15961 cgattaccgc tgccgcaaac agcatttcgc tggtgcgcgc gatttcctcc ggtgccttga
    16021 atttggaatt gtccgcatcc gaatcgcttt caaaaaccaa aacgcttgcc aatccgcgcg
    16081 tgctgaccca aaaccgcaaa gaggccaaaa tcgaatccgg ttacgaaatt cctttcaccg
    16141 taacctcaat cgcgaacggc ggcagcagca cgaacacgga actcaaaaaa gccgtcttgg
    16201 ggctgaccgt tacgccgaac atcacgcccg acggccaaat cattatgacc gtcaaaatca
    16261 acaaggactc gcctgcgcaa tgtgcctccg gtaatcagac gatcctgtgt atttcgacca
    16321 aaaacctgaa tacgcaggct atggttgaaa acggcggcac attgattgtc ggcggtattt
    16381 atgaagaaga caacggcaat acgctgacca aagtccccct gttgggcgac atccccgtta
    16441 tcggcaacct ctttaaaaca cgcgggaaaa aaaccgaccg ccgcgaactg ctgattttca
    16501 ttaccccgag gattatgggt acggccggca acagcctgcg ctattgatgc gtcaaaataa
    16561 gggcatatgt tttacggcat atgccctttc tttatgcttt ttgccgcgac cgaaatgccg
    16621 tcattcccgc gcaggcagaa atccaacttg tccggttccg gttgtttttc gtctcgtaac
    16681 ttttgagccg tcattcccgc gcaggcagaa atccagtccg ttcagtttcg gtcatttccg
    16741 ataaattcct gttgcttttc atttctggat tcccacttt
//
LOCUS       DAWFBO010000028        16654 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFBO010000028 DAWFBO010000000
VERSION     DAWFBO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.028"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..516
                     /locus_tag="WGO87_000673"
     CDS             <1..516
                     /locus_tag="WGO87_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0392064.1"
                     /translation="RGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLD
                     EGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFA
                     NYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRT
                     VGAGVVSSVIA"
     gene            523..598
                     /locus_tag="WGO87_000674"
     tRNA            523..598
                     /locus_tag="WGO87_000674"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:556..558,aa:Trp,seq:cca)
     gene            713..991
                     /gene="secE"
                     /locus_tag="WGO87_000675"
     CDS             713..991
                     /gene="secE"
                     /locus_tag="WGO87_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0392065.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            993..1529
                     /gene="nusG"
                     /locus_tag="WGO87_000676"
     CDS             993..1529
                     /gene="nusG"
                     /locus_tag="WGO87_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0392066.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1630..2064
                     /gene="rplK"
                     /locus_tag="WGO87_000677"
     CDS             1630..2064
                     /gene="rplK"
                     /locus_tag="WGO87_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0392067.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            2064..2759
                     /gene="rplA"
                     /locus_tag="WGO87_000678"
     CDS             2064..2759
                     /gene="rplA"
                     /locus_tag="WGO87_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0392068.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2989..3489
                     /gene="rplJ"
                     /locus_tag="WGO87_000679"
     CDS             2989..3489
                     /gene="rplJ"
                     /locus_tag="WGO87_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0392069.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3547..3918
                     /gene="rplL"
                     /locus_tag="WGO87_000680"
     CDS             3547..3918
                     /gene="rplL"
                     /locus_tag="WGO87_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0392070.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            4108..8286
                     /gene="rpoB"
                     /locus_tag="WGO87_000681"
     CDS             4108..8286
                     /gene="rpoB"
                     /locus_tag="WGO87_000681"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0392071.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YGNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVVRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            8438..12613
                     /gene="rpoC"
                     /locus_tag="WGO87_000682"
     CDS             8438..12613
                     /gene="rpoC"
                     /locus_tag="WGO87_000682"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0392072.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12904..13161
                     /locus_tag="WGO87_000683"
     CDS             12904..13161
                     /locus_tag="WGO87_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392073.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRIGMPINIG"
     gene            13158..13307
                     /locus_tag="WGO87_000684"
     CDS             13158..13307
                     /locus_tag="WGO87_000684"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0392074.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKISSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            13486..13857
                     /gene="rpsL"
                     /locus_tag="WGO87_000685"
     CDS             13486..13857
                     /gene="rpsL"
                     /locus_tag="WGO87_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0392075.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13975..14445
                     /gene="rpsG"
                     /locus_tag="WGO87_000686"
     CDS             13975..14445
                     /gene="rpsG"
                     /locus_tag="WGO87_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0392076.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14464..16569
                     /gene="fusA"
                     /locus_tag="WGO87_000687"
     CDS             14464..16569
                     /gene="fusA"
                     /locus_tag="WGO87_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0392077.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4405 a         3724 c         4344 g         4181 t
ORIGIN      
        1 cgtggtacag tagtaaccgg ccgtgtagag cgcggtatca tccacgttgg tgacgagatt
       61 gaaatcgtcg gtctgaaaga aacccaaaaa accacttgta ccggtgttga aatgttccgc
      121 aaactgctgg acgaaggtca ggcgggcgac aacgtaggcg tattgctgcg cggtaccaaa
      181 cgtgaagacg tggaacgcgg tcaggtattg gctaaaccgg gtactatcac tcctcacacc
      241 aagttcaaag cagaagtata cgtactgagc aaagaagagg gcggccgcca taccccgttc
      301 ttcgccaact accgtcccca attctacttc cgtaccaccg acgtaaccgg cgcggttact
      361 ttggaagaag gtgtggaaat ggtaatgccg ggcgagaacg taaccatcac cgtagaactg
      421 attgcgccta tcgctatgga agaaggtttg cgctttgcga ttcgcgaagg cggtcgtacc
      481 gtgggtgccg gcgtggtttc ttctgttatc gcttaagttt agaggccaat agctcaattg
      541 gtagagtatc ggtctccaaa accgagggtt gggggttcga gaccctcttg gcctgccaaa
      601 taaaaaatta accggccttg tgtcggttaa tttttttgta tttgttattt agtaaactct
      661 cttgccattt acatggattg agaatagaca gatgctatga tggataaata atatgacaga
      721 acatacgcct gaaaaaaaga acgttaaagt ggatcaactg gttgttcaag ataaagaatc
      781 tgcatctaat tccggtaagg aagggttttt tgcatatttc tcaaattctt ggtccgaatt
      841 caaaaaggtg gtttggccta agcgtgaaga tgctgtcaga atgactgtat ttgttatagt
      901 gtttgttgct gtgctttcta tatttatcta tgcggcagat acagcaattt cgtggttatt
      961 ttttgatgta ttgctgagaa gggaaggttg agatgtcgaa aaaatggtat gttgtacagg
     1021 cgtattcggg gtttgagaag aatgtccaac gaatattgga agagcgcatt gcccgtgagg
     1081 agatgggaga ttatttcgga caaattctgg tgcctgtaga gaaagttgtt gatatccgca
     1141 atggtcgtaa gactattagt gaaagaaagt catatcctgg ttatgtgcta gttgagatgg
     1201 aaatgacaga tgactcttgg catcttgtaa aaagcacccc ccgtgtttcc ggttttattg
     1261 gagggagggc taatagacct acgccgatta gtcagagaga ggctgaaatt attttacagc
     1321 aggttcagac cggcatagag aagccgaaac caaaagttga atttgaggtc ggtcaacagg
     1381 ttcgtgtaaa tgaagggccg tttgcggatt ttaacggggt ggttgaggag gtcaattatg
     1441 aacggaataa gttacgcgtg tctgttcaga tatttggtag agaaacaccc gttgagctgg
     1501 agttcagcca ggttgaaaag attaactgat ttttatactt gaaaaaaaag caataagagg
     1561 atagaatcaa aaattaactt ggggagcgga aatggttccg cgtcttaccc gtttttagga
     1621 gttcgttaag tggcaaagaa aattatcggc tatattaaac tgcaaattcc tgcaggtaaa
     1681 gccaatccat ctcctccggt tggtcctgct ttgggtcagc gcggtttgaa tattatggaa
     1741 ttttgtaagg catttaatgc tgcaacccaa ggtatggagc ctggcttacc gattccggtt
     1801 gtgattactg catttgcaga taaatcattc acatttgtga tgaaaacccc gccagcttct
     1861 atcttgttga aaaaggctgc cggtttgcaa aaaggtagtt ctaatcctct gaccaacaaa
     1921 gtgggtaaat tgacccgtgc ccagttggaa gaaattgcta aaactaaaga tcctgatttg
     1981 actgctgctg acttggatgc ggctgtccgt actatagcag gttctgctcg ctcaatgggc
     2041 ttggatgtgg agggtgttgt ataatggcta aagtatctaa acgcttgaaa gctcttcgct
     2101 cttctgtgga agccaataaa ttatatgcaa ttgatgaagc aattgctttg gtaaaaaaag
     2161 cagcgactgc taaatttgac gagtctgttg acgtatcttt caacttgggc gttgatccgc
     2221 gtaaatctga ccaagttatc cgtggttcgg tcgttctgcc taaaggcacc ggtaagataa
     2281 cccgtgtggc tgtatttact caaggtgcaa atgcagaagc tgctaaagaa gctggtgcag
     2341 atatcgtcgg tttcgaagat ttggctgctg aaatcaaagc aggcaatctg aactttgacg
     2401 ttgttattgc ttctcccgat gcaatgcgta ttgttggtca attgggtacc atcttgggtc
     2461 ctcgtggtct gatgccaaac cctaaagtag gtacggttac ccctaatgtt gccgaagcag
     2521 taaaaaatgc aaaagcaggt caagtgcagt accgtaccga caaggcaggt attgttcatg
     2581 caacgattgg tcgtgcttca ttcgctgaag ctgatttgaa agagaacttt gatgcgttgc
     2641 tggatgctat cgttaaagcc aagcctgctg ccgctaaagg tcagtatctg aaaaaagttg
     2701 ctgtgtctag caccatgggt ttgggtattc gcgttgatac atcaagcgta aataactaat
     2761 cttaaggaat tttcaagcag tttggttttc tgggctgctt gaatttgggc tacttaaaat
     2821 taagtagatg tccaagaccg tagggatcgt aagatttaat cgtaactgcc ctacgcagac
     2881 ggtagtcctg aaacacattg caagattgct tgtaagatgt ctttttaggt taccgcgctg
     2941 gtgggatatc gttttggtat cctgtttata aacagtggga ggtagacctt gagtctcaat
     3001 attgaaacca agaaagtggc ggtcgaggaa attagcgcgg caattgctaa cgctcaaacc
     3061 ctcgtagtcg ctgaatatcg cggtatcagt gtttccagta tgactgagct tcgtgcgaat
     3121 gcacgtaaag aaggcgttta tttgcgtgtt ctgaaaaata ctttggctcg tcgtgcagtg
     3181 caaggtactt catttgcaga attggccgat caaatggttg gtccgttggt ttacgctgct
     3241 tctgaagatg ctgttgctgc tgctaaagtg ttgcaccaat tcgcgaaaaa agatgacaaa
     3301 attgtcgtta aagccggttc ttacaatggc gaagtaatga atgctgctca ggttgctgag
     3361 ttggcttcta ttccgagccg cgaagagctg ttgtccaaac tgttgttcgt tatgcaagct
     3421 cctgtatcgg gctttgcgcg cggtttggct gctttggcag agaaaaaagc cggcgaagaa
     3481 gccgcttaat cgattttgtt tctgttaatc aattattttt taatacaata tttggagtaa
     3541 aatagcatgg ctattactaa agaagacatt ttggaagcag ttggttcttt gaccgtaatg
     3601 gaattgaacg acttggttaa agcttttgaa gaaaaattcg gtgtttctgc tgctgctgtt
     3661 gcagttgcag gtcctgctgg tgccggtgct gccgatgctg aagaaaaaac cgaatttgat
     3721 gtcgttttgg cttctgccgg cgatcaaaaa gtcggcgtga ttaaagttgt ccgtgcaatt
     3781 accggtttgg gtctgaaaga agctaaagac atcgttgacg gcgcacctaa aaccattaaa
     3841 gagggtgttt ctaaagctga agccgaagac atccaaaaac aactggaaga agcaggcgct
     3901 aaagtcgaaa tcaaataatt tgatgcttct tatggaggct ggcagttttc tgccagcctt
     3961 attttgcttc ttaaaataaa catcaagtat tgtttacatt tatttgcata gtttttatca
     4021 agtcattgca aataaatgta aatatcagat tgatgcgtac cgttgtttca gacggcctat
     4081 tattgaaaat tacttttcgg agtgtgtatg aactattcgt ttaccgagaa aaaacgtatc
     4141 cgtaagagtt ttgcaaagcg ggaaaatgtt ttggaagttc ctttcttgct agcaacccaa
     4201 attgattctt atgcgaagtt tttgcagctg gaaaatgctt ttgacaaacg taccgatgac
     4261 ggtctgcagg cagcatttaa ttctattttc ccgattgtga gccataacgg ttatgcgcga
     4321 ttggagtttg tgcattacac attgggcgag cctttgttcg atattcccga atgtcagttg
     4381 cgcggaatca cttatgcagc ccccttgcgc gcgcgtatcc gtttggtgat tttggataag
     4441 gaagcatcta aaccgacggt aaaagaagtt cgtgaaaacg aagtgtatat gggcgaaatt
     4501 ccgttgatga ccccgagcgg ttcttttgtg attaacggca cagagcgtgt gattgtctcc
     4561 cagttgcacc gttcgcccgg cgtattcttc gagcatgaca aaggtaagac gcactcttcc
     4621 ggcaagttgt tattctccgc ccgtatcatt ccctaccgcg gttcatggtt ggattttgaa
     4681 tttgacccga aagatttgct gtatttccgt atcgaccgcc gccgtaaaat gccggtaacg
     4741 attttgttga aggctttggg ctacggcaat gagcaaatct tggatatttt ctacgacaaa
     4801 gaaacgttct atttgtcttc aaacggtgtt caaaccgatt tggtcgcagg ccgtctgaaa
     4861 ggcgaaactg ccaaggtcga tatcttggat aaagaaggca atgtattggt tgccaaaggt
     4921 aagcgcatta ctgcgaaaaa tatccgtgat attaccaatg caggcctgac ccgtttggat
     4981 gtagaaccgg aaagcctgct gggtaaagca ttggctgccg atctgattga ttcggaaacc
     5041 ggcgaggtat tggcttctgc caatgatgaa attacagaag agttgttggc caaatttgat
     5101 atcaacggcg taaaagaaat tacgaccctt tacatcaatg agttggatca gggtgcttat
     5161 atctccaaca ccttgcgtac ggatgagact gccggccggc aggcggcgcg tgttgcgatt
     5221 taccgtatga tgcgtccggg cgaaccgccc accgaagagg cggtcgagca attgtttaac
     5281 cgcttgttct tcagtgaaga cagctacgat ttgtcccgcg taggtcgtat gaaatttaat
     5341 acgcgcacat acgaacaaaa actgtccgaa gcccaacaaa actcttggta cggccgcctg
     5401 ctgaacgaaa cgtttgccgg tgctgccgac aaaggcggtt atgtcctgag cgtcgaagat
     5461 attgtcgcct cgattgcgac tttggtcgag ttgcgtaacg gccatggtga agttgacgat
     5521 atcgatcact tgggcaaccg ccgagtacgt tcggtaggcg agctgactga aaaccaattc
     5581 cgcagcggtt tggcccgtgt ggaacgtgct gtaaaagaac gtttgaatca ggcggaatca
     5641 gaaaacttga tgccgcacga tttgattaat gcaaaacctg tttctgccgc tattaaagaa
     5701 ttcttcggct ccagccaatt gagtcagttt atggatcaga ccaacccctt gtctgaagta
     5761 acccataaac gccgcgtatc cgcattgggt ccgggcggtt tgacccgcga acgtgccgga
     5821 tttgaggtgc gggacgtgca tccgacccac tacggccgcg tatgtccgat tgaaacgcct
     5881 gaaggtccga acatcggttt gatcaactca ctgtccgttt atgcgcgcac caatgattac
     5941 ggtttcttgg aaacgcctta ccgccgcgtt atcgacggca aagtaaccga ggaaatcgat
     6001 tacttgtctg ccatcgaaga aggccgctat gtgattgcac aggcgaatgc cgatttggat
     6061 tcagatggca atctgattgg cgatttggtt acctgtcgtg aaaaaggcga aaccattatg
     6121 gcaacgcccg accgcgtcca atatatggac gtggcaaccg gccaggtggt atccgttgcg
     6181 gcatccctga ttccgttctt ggaacatgat gacgcgaacc gcgcattgat gggtgccaac
     6241 atgcaacgtc aggcagtgcc ttgcttgcgt cctgaaaaac cgatggtcgg taccggtatc
     6301 gagcgttccg ttgccgttga ctctgctact gcaatcgttg cccgccgagg cggcgtggtc
     6361 gagtatgtcg atgccaaccg cgtcgtcgtt cgtgttcatg acgatgaagc gactgccggc
     6421 gaagtgggtg tcgatattta caacttggtt aaatttaccc gttccaatca atctactaac
     6481 atcaaccaac gtccggctgt caaagcaggc gatgttttgc aacgcggcga tttggtggcc
     6541 gatggcgcgt ccaccgattt gggcgaattg gctttgggtc aaaatatgac catcgccttc
     6601 atgccgtgga acggttacaa ctacgaagac tcgattctga tttccgaaaa agtggctgcg
     6661 gacgaccgct atacttcgat tcacattgag gaattgaatg tcgttgcccg cgataccaag
     6721 ctgggtgcgg aagacattac ccgcgatatt ccgaacttgt ccgagcgtat gcaaaaccgt
     6781 ttggacgaat ccggtatcgt ttacatcggt gcggaagtag aagccggcga tgtgttggta
     6841 ggcaaggtaa cgcctaaagg cgaaacccag ctgacgccgg aagaaaaact gctgcgcgcc
     6901 atcttcggtg aaaaagcatc tgacgtaaaa gatacttcat tgcgtatgcc taccggcatg
     6961 agcggtaccg ttatcgacgt tcaagtcttc actcgtgaag gtattcaacg cgacaaacgt
     7021 gctcaatcca ttatcgattc cgagttgaaa cgctaccgtt tggatttgaa cgaccaattg
     7081 cgtattttcg acaacgacgc attcgaccgt atcgagcgta tgattgtcgg tcagaaagcc
     7141 aacggcggcc cgatgaagct ggccaaaggc agtgaaatca cgaccgaata tctggcgggt
     7201 ctgccgagca ggcacgattg gttcgatatc cgtctgaccg atgaagattt ggccaagcag
     7261 ttggaactga ttaaagtgag cctgcaacaa aaacgcgaag aagcggacga gttatacgaa
     7321 atcaagaaga aaaaactgac ccaaggcgac gaattgcaac ccggcgtaca aaaaatggtg
     7381 aaagttttta tcgccatcaa acgccgtctg caagccggcg acaaaatggc gggccgccac
     7441 ggtaacaaag gcgtggtatc gcgcattctg ccagtggaag acatgcctta catggcggac
     7501 ggccgtccgg tagacatcgt actgaaccca ttgggcgtac cttcccgtat gaacatcggt
     7561 cagattttgg aagttcactt gggttgggca gcaaaaggta tcggcgagcg tatcgaccgt
     7621 atgctgaaag agcaacgcaa agcaggcgag ttgcgcgagt tcttgaacag actctacaac
     7681 ggcagcggta agaaagaaga tttggatgcc ctgactgatg aagaaatcat cgaactggcc
     7741 tccaacctgc gcaaaggtgc atctttcgcc tctcctgtat tcgacggtgc gaaagagtct
     7801 gaaatccgcg aaatgctgaa cttggcttat ccgagcgacg atcctgaggt tgaaaaactg
     7861 ggcttcaacg acagtaaaac ccaaatcacg ctgtatgacg gccgttcagg cgaagcattt
     7921 gaccgcaagg ttacagtagg tgtgatgcac tatctgaaac tgcaccactt ggttgacgaa
     7981 aaaatgcacg cccgttctac cggtccgtac agtctggtta cccagcagcc tttgggcggt
     8041 aaagcccagt tcggtggcca acgtttcggc gagatggagg tttgggcatt ggaagcatac
     8101 ggcgcggcat acacgctgca agagatgttg actgtgaagt ctgacgacgt gaacggtcgt
     8161 accaaaatgt acgaaaacat cgtcaaaggc gaacacaaaa tcgatgccgg tatgcccgag
     8221 tccttcaacg tattggtcaa agagattcgc tcactgggct tggatatcga tttggaacgc
     8281 tactgatacg ggtttcagac ggcataaggg gagctgttct gcaggtatgc ggggcagccg
     8341 acaatgttta aaaacgaaat gccgtctgaa aacactgtac ctctatccat atcgaaaatc
     8401 cgccatgcgg taaaaatact tccttcaagg agcaaaaatg aatttgttga acttatttaa
     8461 tccgttgcaa actgccggca tggaagaaga gtttgatgcc atcaaaatcg gtattgcctc
     8521 tcccgaaacc atccgctcat ggtcttatgg cgaagtcaaa aaacctgaaa ccatcaacta
     8581 ccgtacgttc aaacctgagc gtgacggttt gttctgtgcc aaaatctttg gcccggtcaa
     8641 agactacgaa tgcttgtgcg gaaaatacaa acgcttgaaa tttaaaggtg taacttgtga
     8701 aaaatgcggc gtggaagtaa ccctgtccaa agtgcgccgc gaacgcatgg gccatatcga
     8761 attggctgcg cccgtcgcac atatttggtt cttaaaatcc ctgccttccc gcttgggtat
     8821 ggtgttagac atgactttgc gcgatatcga acgcgtattg tactttgaag catttgtggt
     8881 aaccgatccc ggtatgactc cgctgcaacg ccgccaattg ctgactgaag acgattacta
     8941 caacaagctg gacgaatacg gcgacgattt cgatgccaaa atgggtgcgg aaggtatccg
     9001 cgaattgctg cgtaccctga atgtagcggg cgaaatcgaa atcctgcgcc aagagttgga
     9061 atcgaccggt tccgacacca aaatcaaaaa aatcgccaaa cgcttgaaag tattggaagc
     9121 tttccatcgt tccggtatga aactggaatg gatgattatg gatgtgctgc cggtattgcc
     9181 gcctgatttg cgtccgttgg ttccattgga tggtggtcgt tttgccactt ccgatttgaa
     9241 cgatttgtac cgccgcgtta ttaaccgtaa caaccgtctg aaacgtctgt tggaactgca
     9301 tgctcctgac atcatcgtcc gcaacgaaaa acgtatgttg caagaagcag ttgactcgct
     9361 gttggataac ggccgtcgcg gtaaagccat gaccggcgcc aacaaacgcc cgctgaaatc
     9421 attggcagac atgattaaag gtaagggtgg ccgcttccgt caaaacctgc tgggcaaacg
     9481 tgtggactac tccggccgtt ccgtgattac cgtaggcccg tacctgcgtc tgcaccaatg
     9541 cggtttgccg aaaaaaatgg ctttggaact gttcaaaccg ttcattttcc acaaattgga
     9601 aaaacaaggt ttggcctcta ccgttaaagc agcgaaaaaa ttggtagagc aagaagtacc
     9661 ggaagtatgg gacatcttgg aagaagtcat ccgcgaacat ccgattatgc tgaaccgtgc
     9721 gccgaccctg caccgtttgg gtattcaagc gttcgaacct atcttgattg aaggtaaagc
     9781 gattcagttg cacccattgg tgtgtgctgc gttcaacgcc gactttgacg gcgaccaaat
     9841 ggcggtacac gttccattga gcttggaagc acaaatggaa gcacgcacgc tgatgctggc
     9901 ttcaaacaac gtattgtctc ctgccaacgg cgaaccgatt atcgtacctt cccaagacat
     9961 cgtattgggt ctgtactaca tgacccgcga ccgtatcaat gccaaaggcg agggcagcct
    10021 gtttgccgat gtgaaagaag tgcatcgcgc ataccatacc aaacaggttg aactgggtac
    10081 gaaaattacc gtacgtctgc gcgaatgggt gaaaaacgaa gcaggtgagt ttgagcctgt
    10141 cgttaaccgt tacgaaacaa ccgtcggccg tgcattgttg agcgaaatcc tgccgaaagg
    10201 cctgccgttt gaatatgtca acaaagcgtt gaagaaaaaa gaaatttcta aactgattaa
    10261 cgcatcgttc cgcctgtgcg gcttgcgcga tacggttatc tttgctgacc acctgatgta
    10321 caccggtttc ggatttgcgg caaaaggcgg tatttccatt gccgttgacg atatggaaat
    10381 tccaaaagaa aaagcggcct tgctggctga agccaatgcc gaggttaaag aaatcgaaga
    10441 ccaataccgt caaggtttgg ttaccaacgg cgaacgctac aacaaggtgg tcgatatttg
    10501 gggtcgtgcc ggcgataaaa tcgctaaagc gatgatggac aacctgtcca aacagaaagt
    10561 tatcgaccgt gccggcaacg aagtcgatca agagtcattc aactccatct atatgatggc
    10621 tgactccggt gcccgtggtt ctgcggctca gattaaacag ttgtccggta tgcgcggttt
    10681 gatggcgaaa cctgacggct cgattattga aacgccgatt acctcaaact tccgcgaagg
    10741 tctgaccgta ttgcaatact ttattgcgac ccacggtgcg cgtaagggtt tggcggatac
    10801 cgcattgaaa accgcgaact ccggttacct gacccgtcgt ctggtagacg taactcaaga
    10861 tttggtcgtt gttgaagacg attgcggtac ttcagacggc tttgtcatga aggcagtggt
    10921 acaaggcggt gatgtgattg aagcattgcg cgatcgtatt ttgggccgtg ttaccgcgtc
    10981 tgacgttgtc gatccgtcaa gtggcgaaac attggttgaa gcaggcacgt tgttgactga
    11041 aaaactggtg gatatgattg accaatccgg tgtcgatgaa gtcaaagtcc gtacgccgat
    11101 tacttgtaaa acccgtcacg gtttgtgcgc gcactgttac ggccgcgact tggcgcgcgg
    11161 caaactggtc aatgccggcg aggcagtcgg cgtgattgcc gctcagtcta tcggcgaacc
    11221 gggtacccag ttgaccatgc gcacgttcca catcggtggt gcggcatccc gtgcggcagc
    11281 agccagccaa gtggaagcca aatccaacgg tacggcacgt ttcagcagcc agatgcgtta
    11341 cgttgccaac aacaaaggtg agttggttgt catcggtcgc tcttgcgaag tcgtgattca
    11401 tgatgacatc ggccgtgaac gcgaacgcca caaagtacct tacggtgcca tcctgctggt
    11461 acaagacggt atggccatta aagccggtca aaccttggca acctgggatc cgcatacccg
    11521 tccgatgatc accgaacatg caggtatggt gaaatttgag aacgtggaag agggtgttac
    11581 cgttgcgaaa caaactgacg atgtaaccgg tttgtccact ttggtggtga ttgacggtaa
    11641 acgccgttcc agcagcgcgt ccaaactgct gcgtccgact gtaaaattgt tggatgaaaa
    11701 cggcgtggaa atctgtattc ccggtacgtc cactccggtt tcgatggcat tccccgttgg
    11761 tgcggtgatt accgtacgcg aaggtcagga aatcggtaaa ggcgacgtat tggcgcgtat
    11821 cccgcaagcc tcttccaaaa cccgcgacat taccggtggt ctgccgcgcg ttgccgaatt
    11881 gtttgaagca cgcgtgccga aagatgccgg tatgttggcg gaaattaccg gtaccgtttc
    11941 cttcggcaaa gagaccaaag gcaagcaacg tctgattgtt actgacgtgg acggtgtagc
    12001 atacgagacc ttgatttcca aagagaaaca aattctggta cacgacggtc aagtggtaaa
    12061 ccgcggtgaa accatcgtgg acggagctgt tgatccgcat gatattctgc gtttgcaagg
    12121 tatcgaagca ctggcacgct acattgtcca agaggtgcaa gaggtttacc gtctgcaagg
    12181 tgtgaagatt tctgataaac acatcgaagt catcatccgt caaatgttgc gccgtgtgaa
    12241 cattgcggat gccggcgaaa ccgggttcat taccggagag caggtcgaac gcggcgatgt
    12301 gatggcggcc aatgaaaaag ctttggaaga aggcaaagag ccggcacgtt acgaaaacgt
    12361 attgctgggt attaccaaag cctccctgtc caccgacagt ttcatttctg ccgcatcgtt
    12421 ccaagaaacg acccgtgttc tgaccgaagc cgcgattatg ggcaaacaag acgaactgcg
    12481 cggtttaaaa gagaacgtca tcgtcggccg cctgattcct gccggtaccg gtttgactta
    12541 tcaccgcagc cgtcatcagc aatggcaagg ggtagagcag gagactgctg aaacccaagt
    12601 aacggatgaa taatcttcgg cgcgtttatt caatacaaaa ccgcaagctt taagcttgcg
    12661 gtttttcttt gtccgattaa ggcaaaaaca agcgttttcg tcattttgag gcgtgtggat
    12721 tattccttag gtattttcgg gccggagacc aacgaggtgg cgggtgtcgt cggtacgtcc
    12781 ggagaccaaa ataactttgc caaggatgtt ggtttcggcg gtcaaaaaag tagcgtctta
    12841 atgttttcca tttaaacaaa tgtcgtctga aacttcagac ggcatttcct ttaagaaata
    12901 aatatgaaac ccagaaatct cttttttgca ggctgcctgc tgacttcggc gacgtttgcc
    12961 gaggatatcg gcgtacctgt cgaactgatt aacgtcggta atcggattgc gatgccgtct
    13021 gaaggggaaa gcctcgccct cctgccgttt gccgaggatg taccgccggt tcgcgatgca
    13081 atgccgtctg aagttcctaa aagcgcggca ggcggcgatg ttcggggtga ccggatagga
    13141 atgccgatta acatcggatg agcgcggctt tatggcataa aaaactgtcg tggaaaggat
    13201 ttacacccca aataaatttc cgttacaaca agatcagcag caatatgccc gccttttatt
    13261 cgcgcagcgg caaggaacgg tttgtcagta tagaaaaaac gtattgacag tattttcttc
    13321 agtcgtccga ctgattgtga gggatgtcgg taaatattta tcggcaaaca agaaaatcat
    13381 ctttcttctt gtcgttatgc ttgactgtat acttgcaata aaaatataat tccactcttg
    13441 ccgacatggt gtcggcaagt atttaactca acaggacgag aaaatatgcc aactatcaac
    13501 caattggtac gcaaaggccg tcaaaagccc gtgtacgtaa acaaagtgcc cgcactggaa
    13561 gcctgcccgc aaaaacgcgg cgtgtgcacc cgtgtataca cgactacccc taaaaaacct
    13621 aactctgcat tgcgtaaagt atgtaaagtc cgcctgacca acggttttga agtcatttca
    13681 tacatcggcg gcgaaggcca caacctgcaa gagcacagtg tcgtattgat tcgcggcggt
    13741 cgtgtaaaag acttgccagg tgtgcgttac cacactgtac gcggttcttt ggatactgca
    13801 ggcgttaaag accgtaaaca agcccgttcc aaatacggtg ctaagcgtcc taaataatta
    13861 ctgggactta aataggcacg tcggccgcct aagctgaaca acggccgagt aagtgaatac
    13921 tcaattgggt attcatggga atagacccga ctgaatagat taaaggaaat taaaatgcca
    13981 agacgtagag aagtccccaa gcgcgacgta ctgccagatc ctaaattcgg cagcgtcgag
    14041 ttgaccaaat tcatgaacgt attgatgatt gacggtaaaa aatccgttgc cgagcgtatc
    14101 gtttacggtg cgttggaaca gattgagaaa aaaaccggca aagtagcaat cgaagtattt
    14161 aacgaagcca ttgcaaacgc caaacctatc gtggaagtga aaagccgccg tgtaggtggt
    14221 gcaaactacc aagttcctgt tgaagttcgt ccttcacgcc gtttggcttt ggcaatgcgc
    14281 tgggttcgcg atgcggcccg caaacgtggt gagaaatcca tggacctgcg tttggcaggc
    14341 gaattgattg atgcgtccga aggccgtggc ggtgcgttga aaaaacgtga agaagtacac
    14401 cgtatggctg aagccaacaa agcattctct cacttccgtt tctaattttg aaaggctaat
    14461 aaaatggctc gtaagacccc gatcagcctg taccgtaaca tcggtatttc cgcccatatc
    14521 gatgcgggta aaaccacgac gacagaacgt attttgttct ataccggttt gacccacaag
    14581 ctgggcgaag tgcatgacgg tgcggctact accgactaca tggaacaaga gcaagagcgc
    14641 ggtattacca ttacctccgc tgccgttact tcctactggt ccggtatggc gaaacaattc
    14701 cccgagcacc gcttcaacat catcgacacc ccgggacacg ttgactttac cgtagaggta
    14761 gagcgttcta tgcgtgtatt ggacggcgcg gtaatggttt actgcgcggt gggcggtgtt
    14821 caaccccaat ctgaaaccgt atggcggcaa gccaacaaat accaagtgcc gcgcttggcg
    14881 tttgtcaata aaatggaccg tcagggtgcc aacttcttcc gcgttgtcga gcaaatgaaa
    14941 acccgtttgc gcgcaaaccc tgtacctatc gtcattccgg ttggtgcgga agacaacttc
    15001 agcggtgtgg ttgatttgtt gaaaatgaaa tccatcattt ggaatgaagc cgataaaggt
    15061 acaaccttta cctatggcga tattcctgcc gaattggtcg aaactgccga agaatggcgt
    15121 caaaatatga ttgaagccgc agccgaagcc agcgaagaac tgatggacaa atacttaggc
    15181 ggcgacgagc tgaccgaaga agaaatcgta ggcgcgttgc gtcaacgtac tttggcaggc
    15241 gaaattcagc ctatgctgtg tggttctgca tttaaaaaca aaggtgttca acgtatgttg
    15301 gacgcagttg tagaattgct gccagctcct accgatattc ctccggttca aggtgtcaac
    15361 ccgaataccg aggaagccga cagccgtcaa gccagcgatg aagagaaatt ctctgcattg
    15421 gcgttcaaaa tgttgaacga caaatacgtc ggtcagctga cctttatccg cgtttactca
    15481 ggcgtagtaa aatccggcga taccgtattg aactccgtaa aaggcactcg cgaacgtatc
    15541 ggtcgtttgg tacaaatgac tgccgcagac cgtactgaaa tcgaagaagt acgcgccggc
    15601 gacatcgcag ccgctattgg tctgaaagac gttactaccg gtgaaacctt gtgtgcggaa
    15661 agcgcgccga ttatcttgga acgcatggaa ttccccgagc cggtaatcca tattgccgtt
    15721 gagccgaaaa ccaaagccga ccaagagaaa atgggtatcg ccctgaaccg cttggctaaa
    15781 gaagaccctt ctttccgtgt ccgtacagac gaagaatccg gtcaaaccat tatttccggt
    15841 atgggtgagc tgcacttgga aattattgtt gaccgtatga aacgcgaatt cggtgtggaa
    15901 gcaaatatcg gtgcacctca agtggcttac cgtgaaacta tccgcaaagc cgttaaagct
    15961 gaatacaaac atgcaaaaca atccggtggt aaaggtcaat acggtcacgt tgtgattgaa
    16021 atggaaccta tggagccggg tggtgaaggt tacgagttta tcgatgaaat taaaggtggt
    16081 gtgattcctc gcgaattcat tccgtctgtc gataaaggta tccgcgatac gttgcctaac
    16141 ggtatcgttg ccggctatcc tgtagttgac gtacgtatcc gtctggtatt cggttcttac
    16201 catgatgtcg actcttccca attggcattt gaattggctg cttctcaagc gtttaaagaa
    16261 ggtatgcgtc aagcatctcc cgccctgctt gagccgatta tggcagttga agtggaaacc
    16321 ccggaagaat acatgggcga cgtaatgggc gacttgaacc gccgtcgcgg tgttgtattg
    16381 ggtatggatg atgacggtat cggcggtaaa aaagtccgtg ccgaagtacc tctggcagaa
    16441 atgttcggtt actcgaccga cctgcgttct gcaacccaag gccgcgctac ttactctatg
    16501 gagttcaaga aatattctga agctcctgct cacatagctg ctgctgtaac tgaagcccgt
    16561 aaaggctaat caggcaaata ggccgtccga aaggctgaaa tgatttttca gacggcattg
    16621 ttctttaatc gatctttaat gtaaaggaat tagc
//
LOCUS       DAWFBO010000029        16458 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFBO010000029 DAWFBO010000000
VERSION     DAWFBO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.029"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            210..1274
                     /locus_tag="WGO87_000688"
     CDS             210..1274
                     /locus_tag="WGO87_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0392078.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1415..1738
                     /locus_tag="WGO87_000689"
     CDS             1415..1738
                     /locus_tag="WGO87_000689"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0392079.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1810..2133
                     /locus_tag="WGO87_000690"
     CDS             1810..2133
                     /locus_tag="WGO87_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0392080.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2180..2383
                     /gene="tatA"
                     /locus_tag="WGO87_000691"
     CDS             2180..2383
                     /gene="tatA"
                     /locus_tag="WGO87_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0392081.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2387..3073
                     /gene="tatB"
                     /locus_tag="WGO87_000692"
     CDS             2387..3073
                     /gene="tatB"
                     /locus_tag="WGO87_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0392082.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANPASDGISDVMPSERSYTSAETLGDDRQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3087..3857
                     /gene="tatC"
                     /locus_tag="WGO87_000693"
     CDS             3087..3857
                     /gene="tatC"
                     /locus_tag="WGO87_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0392083.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAVITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3910..4515
                     /gene="maf"
                     /locus_tag="WGO87_000694"
     CDS             3910..4515
                     /gene="maf"
                     /locus_tag="WGO87_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0392084.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAATFLNLL
                     SGTGHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIGGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4531..4884
                     /locus_tag="WGO87_000695"
     CDS             4531..4884
                     /locus_tag="WGO87_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0392085.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4874..6680
                     /locus_tag="WGO87_000696"
                     /pseudo
     CDS             4874..6680
                     /locus_tag="WGO87_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
     gene            6870..7547
                     /locus_tag="WGO87_000697"
     CDS             6870..7547
                     /locus_tag="WGO87_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0392086.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7560..8966
                     /locus_tag="WGO87_000698"
     CDS             7560..8966
                     /locus_tag="WGO87_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0392087.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9084..11510
                     /locus_tag="WGO87_000699"
     CDS             9084..11510
                     /locus_tag="WGO87_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0392088.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGSADLIIAVTPPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTACCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLSPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTARDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11555..11800
                     /gene="rpsP"
                     /locus_tag="WGO87_000700"
     CDS             11555..11800
                     /gene="rpsP"
                     /locus_tag="WGO87_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0392089.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11816..12325
                     /gene="rimM"
                     /locus_tag="WGO87_000701"
     CDS             11816..12325
                     /gene="rimM"
                     /locus_tag="WGO87_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0392090.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLHGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12325..13074
                     /gene="trmD"
                     /locus_tag="WGO87_000702"
     CDS             12325..13074
                     /gene="trmD"
                     /locus_tag="WGO87_000702"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0392091.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13090..13455
                     /gene="rplS"
                     /locus_tag="WGO87_000703"
     CDS             13090..13455
                     /gene="rplS"
                     /locus_tag="WGO87_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0392092.1"
                     /translation="MNLIQQLEQEEIARLNKDIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13774..14499
                     /locus_tag="WGO87_000704"
     CDS             13774..14499
                     /locus_tag="WGO87_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0392093.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDTRTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14535..15410
                     /locus_tag="WGO87_000705"
     CDS             14535..15410
                     /locus_tag="WGO87_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0392094.1"
                     /translation="MNPYDLLITPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15487..16413
                     /locus_tag="WGO87_000706"
     CDS             15487..16413
                     /locus_tag="WGO87_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEZ0392095.1"
                     /translation="MKHPKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALI
                     KADPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHI
                     EFIAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETSVNVSG
                     KLYSDALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4269 a         5288 c         3618 g         3283 t
ORIGIN      
        1 gccatctgaa gccgattcgg ttttcagacg gcatcattat ttgacctgac gcaaacagaa
       61 agccttccgc gtgcttaaac acatctcttg tagtaaaata tctaaacaga ttcaactgga
      121 aacggtcagg cttcttcacg ccattcccat ttcaaccgcc gcctgccaga ttcagacggt
      181 attcctactt gaaaacaagg agcgttacaa tgaaagcagc acgtttttac gacaaaggcg
      241 acatccgcat cgaagacatc cccgaaccga ccgtcgcccc cggcaccgtc ggcatcaatg
      301 ttgcctggtg cggcatctgc ggtaccgacc tgcacgaatt catggaaggc ccgattttca
      361 ttccgccttg cggtcatccg cacccgattt ccggcgagtc cgcacccgtc acgatgggac
      421 acgagttctc cggcgtggtc tatgccgtcg gcgaaggcgt ggacgacatc aaagtcggtc
      481 aacacgtcgt tgtcgagcct tacatcgtac acgacgacgt accgaccggc ccgggcagca
      541 actaccacct ctctaaagac atgaacttca tcggcttggg cggctgcgga ggtggcttgt
      601 ctgaaaaaat cgctgtcaaa cgccgttggg tgcatccgat ttccgacaaa atcccgttgg
      661 atcaagccgc tttgatcgaa ccgctgtctg tcggacacca cgcctatgta cgcagcggcg
      721 cgaaagaagg cgatgtcgca ttggtcggcg gtgcaggccc gatcggtttg ctgttggctg
      781 ccgtgctgaa agccaaaggc atcaaagtca tcatcaccga gttgagtaaa gcacgcaaag
      841 acaaagcgcg cgaatccggc gttgccgact acatcctcga cccgtccgaa gtcgatgttg
      901 tcgaagaagt gaaaaaactg accaacggcg aaggcgtgga cgtggcattc gaatgtacca
      961 gcgtgaacaa agtgttggat actttggtcg aagcctgcaa acctgccgcc aatttggtta
     1021 tcgtatccat ctggagccac cctgcaaccg tcaacgtcca cagcgtcgtg atgaaagagc
     1081 tggatgtgcg cggcacgatt gcctactgta acgaccacgc cgaaaccatc aaactggtcg
     1141 aagaaggcaa aatcaacctc gaacccttca tcacccagcg catcaagctg gacgaactgg
     1201 tttccgaagg cttcgagcgt ctgatccaca acaacgaatc cgccgttaaa attattgtga
     1261 gtccaaacct gtaagcaaat tacatttgca acagcacaaa tgccgtctga aacgctcaga
     1321 cggcattttc caacgaagcg caacaggcgt attgcccgag agcagccgtt tagcctatca
     1381 ttccgaacaa acagaaacaa acagaaggaa aatcatggga gattccgtac tatccgccat
     1441 ccaacaaacc atcacccagc gaaaatctgc caatccgtcc gaatcttacg tcgcacagct
     1501 cttgcataag ggcgaagaca aaatcctaaa aaaagtgatt gaagaggcgg gcgaagtgtt
     1561 gatggcatcc aaagacaaaa acccgtccca cctggtttac gaagttgccg acttatggtt
     1621 tcacaccatg attcttctga cacaccacga cctgaaggcg gaagacgtat tggacgaact
     1681 tgcgcgccgc caaggtttgt cgggcttggc cgaaaaagcc gctcgcacag aatcttgaat
     1741 ttatattaaa atccgcactt tcccacattc aatccgtctg accgcttttc agacggcatc
     1801 ggagccgtta tggacaactg tattttctgc aaaatcgccg ccaaagacat tccggcgcaa
     1861 accgtctacg aagacggcga aatggtttgt ttcaaagaca tcaaccccgc tgctccggtt
     1921 catctgctgc tgattcccaa agtccatttc gattcgttgg cacacgccgc gcccgaacat
     1981 cagccccttt tgggaaaaat gatgctgaaa gttcccgaaa tcgccaaagc ggcaggactg
     2041 gcagacggct tcaaaaccct gatcaacacc ggaaaaggcg gcggacaaga ggtcttccac
     2101 ctgcatatac acatcatggg cacacccgta taaaccgtta tttcacaatc aacccctaat
     2161 acttacttaa ggatacatca tgggcagttt ttctctgacg cactggatta tcgtactgat
     2221 tatcgtcgtt ttgatattcg gcaccaaaaa actgcgcaac gtcggcaaag acctcggcgg
     2281 tgcggttcat gacttcaaac aggggctgaa cgaaggcaca gacggcaaag aagcccaaaa
     2341 agacgatgta atcgaacaca aaaaagacga agacaaagcg taatttatgt ttgatttcgg
     2401 tttgggcgag ctggtttttg tcggcattat cgccctgatt gtcctcggcc ccgaacgcct
     2461 gcccgaggcc gcccgcaccg ccggacggct catcggcagg ctgcaacgct ttgtcggcag
     2521 cgtcaaacag gaatttgaca ctcaaatcga actggaagaa ctgaggaagg ccaagcagga
     2581 attcgaagct gccgccgctc aggttcgaga cagcctcaaa gaaatcggta cggatatgga
     2641 gggtaatctg cacgacattt ccaatggtct gaagccttgg gaaaaactgc ccgaacagcg
     2701 cacgcctgct gatttcggtg tcgatgaaaa cggcaatccg cttcccgatg cggcaaaccc
     2761 cgcatcagac ggcatttccg acgttatgcc gtccgaacgt tcctacactt ccgccgaaac
     2821 ccttggagac gacaggcaaa ccggcagtac agccgaaccc gcggaaaccg accaagaccg
     2881 cgcatggcgg gaatacctga ctgcttctgc cgccgcaccc gtcgtacaga ccgtcgaagt
     2941 cagctatatc gataccgctg ttgaaacccc tgttccgcat accacttcgc tgcgtaaaca
     3001 ggcaataagc cgcaaacgcg atttgcgtcc caaatcccgc gccaaaccta aattgcgcgt
     3061 ccgtaaatca taaagagggc aatccggtgt ccgaaacaca aaacgaacaa cccgtccaac
     3121 cgcttgtcga gcatctcatc gagctgcgcc gccgcctgat gtggacggtt gtcggcatct
     3181 tagtctgctt tttcggccta atgccgtttg cccaacaact ctatactttt atcgccgacc
     3241 cgctgatggc aaacctgccc aaagacacca gcatgattgc caccgatgtc atcgcaccgt
     3301 ttttcgtgcc ggtcaaagtt accctgatgg cggcattttt aatttcgctg ccgcatacgc
     3361 tctaccaaat ctgggcattt gtcgcgcccg cactctacca aaacgaaaaa cgcctgatta
     3421 cgccgctcgt cctctccagc gtcagcctgt ttttcatcgg catggcattt gcctactttt
     3481 tggttttccc cgtcattttc aaattccttg ccagcgttac ccctgtcggt gtcaatatgg
     3541 cgacagacat cgacaaatac ctctccttca tcttggggat gtttgtcgca ttcggtacaa
     3601 cgtttgaagt ccccattgtc gttatcctgt taaccaaaat tggtgtggta acaaccgaac
     3661 agctcaaacg cgcccgcccc tatgtgattg tcggcgcgtt tgtcattgcc gcagtcatca
     3721 caccgcccga tgtgatttca caaaccctgc ttgccattcc gctgattctc ttatacgaag
     3781 caggtatttg gttcggacgc tttttcacgc cacgttcaga acaggatggc gacatacagc
     3841 cgcctgcaac aacctgacac tatgccgtcc gaacctccgc ctcataccgc aacagattaa
     3901 ggaatacctt tgaataccct ctatttaggt tcaaacagcc cgcgccgaat ggaaatcctg
     3961 acacagttgg gctatcaggt cgtcaagctg cctgccgaca tcaatgaaac ggtcagacag
     4021 aacgaagacc ctgcccgtta cgttcaaagg atggcagaag aaaaaaaccg aaccgccctg
     4081 accctctttt gcgaaaccaa cggcacaatg cccgatttcc ccctgattac cgccgacacc
     4141 tgcgtcgttt cagacggcat catattgggc aaaccccgct cccaagctga agcggcaacc
     4201 tttctaaacc tgctgtcggg aaccgggcat accgtcctga ctgctgtctg cattcattat
     4261 cgcggcaaaa cgtcaagccg cgtccaaacc aaccgcgtcg ttttcaagcc cctgagttcg
     4321 gaagaaattt ccgcctatgt gcaaagcggc gaaccgatgg acaaagccgg tgcctacgcc
     4381 gtacaaggca taggcggcat ctttatccaa tctatcggag gcagcttcag cggcattatg
     4441 gggctgcccg tttatgaaac cgtttcgatg ttgcaggatt tgggataccg taccccacct
     4501 ttcatccgag cctaacccgt aaagaccgcc gtgaacagac aaaccgctta cctccttacc
     4561 tctttcagcc tgatcgcact gataatcctg tccctttcct gggaactgtg gattgcaccg
     4621 ttgcgcccgg gcggctcgtg gctcgcgctc aaagccctcc ccctctgtct gccgctttca
     4681 ggcatcttga aaaagaaaat ctatacttac caatacagct ccatgctggt tctgatttac
     4741 tttgccgaag ccgtcatgcg cctgttcgac gcctatcccg cagaacagat ttgcgccgcg
     4801 ctttccgcag tattcagcat catcttcttc atatcctgcc tgtcgttcgt caaacaatac
     4861 aaggaaacaa acaatgcccg ctgaaacgac cgtatccggc gcgcaccccg ccgccaaact
     4921 gccgatttac atcctgccct gcttcctttg gataggcatc gtccccttta ccttcgcgct
     4981 caggcagcaa ccgtcgcccg acttttacca cgatgctgca gccgcagccg gcctgattgt
     5041 cctgttgttc ctcacggcag gaaaaaaact gtttgatgtc aaaatccccg ccatcagctt
     5101 ccttctgttt gcaatggcgg cgttttggta tcttcaggca cgcctgatga acctgattta
     5161 ccccggtatg aacgacatcg tctcttggat tttcatcttg ctcgccgtca gcgcgtggac
     5221 ctgccggagc ttggtcgcac acttcggaca agaacgcatc gttaccctgc ttgcctggtc
     5281 gctgcttatc ggctccctgc ttcaatcctg catcgtcgtc atccagtttg ccggctggcc
     5341 ccatgcttca aaacattatt gtttacagcg ggcaaggcgt aatcggacac atcggacagc
     5401 gcaacaacct cggacactac ctgatgtggg gcatactcgc cgccgcctac ctcaacggac
     5461 aacgaaaaat cccgcccgcc ctcggcgcaa tctgcctgat tatgcagacc gccgttttag
     5521 gtttggtcaa ttcgcgcacc atcttgacct acatagccgc catcgccctc atccttccct
     5581 tctggtattt ccgttcggac aaatccaaca ggcggacgat gctcggcata gccgcagccg
     5641 tattccttac cgcgctgttc caattttcca tgaacaccat tctggaaacc tttactggca
     5701 tccgctacga aactgccgtc gaacgcgtcg ccaacggcgg tttcacagac ttgccgcgcc
     5761 aaatcgaatg gaataaagcc cttgccgcct tccagtccgc cccgatattc gggcacggct
     5821 ggaacagttt tgcccaacaa accttcctca tcaatgccga acagcacaac atatacgaca
     5881 acctcctcag caacttgttc acccattccc acaacatcgt cctccaactc cttgcagaga
     5941 tgggaatcag cggcacgctt ctggttgccg caaccctgct gacgggcatt gccgggctgc
     6001 ttaaacgccc cctgaccccc gcatcgcttt tcctgatctg cacacttgcc gtcagtatgt
     6061 gccacagtat gctcgaatat cctttgtggt atgtctattt cctcatcccc ttcggactga
     6121 tgctctttct gtcccccgca gaggcttcag acggcatcgc cttcaaaaaa gccgccaatc
     6181 tcggcatact aaccgcctcc gccgccatat tcgcaggatt gctgcacttg gactggacat
     6241 acacccggat ggttaacgcc ttttcccccg ccactgacga cagtgccaaa accctcaacc
     6301 ggaaaatcaa cgagttgcgc tatatttccg caaacagtcc gatgctgtcc ttttatgccg
     6361 acttctccct cgtaaacttc gccctgccgg aataccccga aacccagact tgggcggaag
     6421 aagcaaccct caaatcacta aaataccgcc cccactccgc cacctaccgc atcgccctct
     6481 acctgatgcg gcaaggcaaa gttgcagaag caaaacaatg gatgcgggcg acacagtcct
     6541 attaccccta cctgatgccc cgatacgccg acgaaatccg caaactgccc gtatgggcgc
     6601 cgctgctacc cgaactgctc aaagactgca aagccttcgc cgccgcgccc ggtcatccgg
     6661 aagcaaaacc ctgcaaatga cccgcgccgg cggatgcgga taccgcccga aatgtaaagc
     6721 tccatgcaag acattgcaaa aaacagcaaa ccggtaggga aatacgctat caaaaaacat
     6781 tgcagccgtg ttaagataaa ccgtcaaaca atcttttcac gccccgcccg aaacagggtc
     6841 ggggcatacc cttacgaaaa ggaaacacca tgagccgcgt attactcgta gatgacgatg
     6901 ccctgctaac cgaactgctg accgaatacc tgagcgccga aggtctgaac gtccgcagcg
     6961 ttcccgacgg ggaagcaggc gtacaggaaa tcctgagcgg gcagtacgat gtagtcgtat
     7021 tggattccat gatgcccaaa atgaacggct tggatgtctt gaaaaacgta cgcgcccgaa
     7081 gcaccgtccc catcatcatg ctgaccgcca aaggcgacga catcgaccga atcatcggct
     7141 tggaaatggg cgcggacgac tatgtcccca aaccctgcac accacgcgaa ctcttggcac
     7201 gcatcaatgc catcctgcgc cgcgcacaac acagcggcga acagaacaac gcacccaaca
     7261 gcatctccgt cagcgatgtc gtcctgtacc ccgccaaacg ccaggcatcc gtcaaagaca
     7321 tgccgctcga actgaccagc accgaattca acctgctcga agtcctgatg cgccatgccg
     7381 gacaggtagt cagcaaagaa accctgtccg tcgaagcact cgaccgcaag ctggcaaaat
     7441 tcgaccgcag tatcgacgta cacatctcca gcatccgcca caagttgggc gatgcctctc
     7501 tgattcaaac cgtacgcggc ttgggctacc tgtttgtcaa aaactgaagc aacgggcaaa
     7561 tgaaactgtt ccaacgcatc ttcgccacat tttgcgcggt tatcgtctgt gcaatctttg
     7621 tggcgagttt ttctttttgg ctggtgcaga acacccttgc cgaaaaccag ttcaaccaac
     7681 gccgcaccat cgaaaccact ttgatgggca gcatcatttc cgcattccgg gcacgcgggg
     7741 acgcgggcgc gcgcgaaatc ctgacggaat ggaaagacag ccccgtctca tcgggcgtgt
     7801 acgttataca gggcgacgag aaaaaagata tcctgaaccg gtatatcgac agctatacca
     7861 tcgaacgcgc ccggcttttc gccgccggac acccgcattc caacctcgtc catatcgaat
     7921 acgaccgctt cggcgaagaa tacctgttct tcaccaaaga ctgggacaaa ctccaagccc
     7981 gccgcctgcc cagccccctg ttgatccccg gcctgccgct cgccccgatt tggcacgaac
     8041 tcatcatatt gtccttcatc atcatcgtcg gactgctgat ggcatatatc ctcgccggca
     8101 acattgccaa acccatcaga atcttaggca acggcatgga cagggtggca aacggagaac
     8161 ttgaaacccg tatctcccaa caggtcgacg accgcgacga cgaattgtcc catcttgcca
     8221 tccaattcga caaaatggtg gaaaaactcg aaaaactcgt tgccaaagaa cgccacctgc
     8281 tccatcacgt ctcccatgaa atgcgttctc cccttgcgcg catgcaggca attgtcggac
     8341 tgattcaggc gcagccccaa aaacaggagc aatatctcaa acggctggaa ggcgaactga
     8401 cccgcatgga tacgctggcc ggggaactgt taaccctgtc ccgtctcgaa acttccaata
     8461 tggctttgga aaaagaaagc ctgaaactcc tgcccttcct gggcaacctg gtagaagaca
     8521 atcaaagcat tgcccagaaa aacggacaaa cggttaccct gtctgccgac ggaaaaatcc
     8581 ccgaaaatac aaccatcctt gccaacgaaa gctacctgta ccgcgccttc gacaacgtca
     8641 tccgcaacgc cgtcaactac agtcccgaag gcagcaccat cctgatcaac atcggacaag
     8701 accacaaaca ctggataatc gacgttaccg acaacggccc cggcgtggac gaaatgcagc
     8761 tcccgcacat cttcaccgct ttctaccgtg cagactccag tgcgaacaaa cccggaacag
     8821 gactggggct tgcattgacc caacatatta ttgaacagca ctgcggaaaa atcatcgccg
     8881 aaaacatcaa accgaacggt ctgcggatgc gctttatcct gcccaagaaa aaaaccggtt
     8941 ccaaaacaga aaaaagtgcg aactgaccat aatgccgtct gaaccggctt cagacggcat
     9001 tgcacaaaca gttaccccaa aacaaacaca cagcgggtgc atctccaaca atcacgcaca
     9061 taccgtccgc aaaggaatat atcatgtcgg cacaaaccga tccgggctac tttttcatgc
     9121 ccaaccacat tatcctgata ggcgcgagcg aacgtccgta cagcctgggc gaacgcgtac
     9181 tcagcaacct gctgagtacg ccctttcaag gaaaaatcac ccccgtaaac ccgcgccacc
     9241 acaccatagc cgggcttccc gcctacacca gcctcaacaa aatccccggc agtgcagacc
     9301 tgattattgc cgttaccccg cccgacagtt acgacaccct cttcaaaacc tgccgcaaaa
     9361 agcagctccg acacatcatc ctcatacagg actgggacag cctgtctgcc gcagaactgc
     9421 acaccgccga aactgccatc cgcaaacacc acggcaacgg actcaacatc accgcctgca
     9481 ccaccgcagg catccaactg ccctcactcg gacttaacat cagtacccaa gacggatacg
     9541 ccgcaggcca taccgccata ctgaccggca atgccgccgt cagccgccaa atcgacaaca
     9601 tcctgaacaa actccgtcaa ggcacatccc gccacatcag cctgcatccc ggcatcagcc
     9661 ccatcacatc cgccgattgg ctcaaccgct tcggacacag cctgcacacc aaaaccgccg
     9721 tcctacacca caaccctgaa gaggatcagc gcaaactgtt cagcgcaatc cgccaattta
     9781 cccgccatac gccgctgatt ctccacatca cctgccgcac gacagaaacc gaccgtgccg
     9841 tactgcactg cctcgcccga cactgcaact tcctcatcag tttcaacgcc gacgacctcg
     9901 aagccgcact gcgcgcccaa ctgtccgacc ttccacccct gtcccgactc gacatcctgt
     9961 ccgacacgcc tgccgaatgg ctgaacgcgc acgcgccaaa aaccctcgcc ctccactttc
    10021 ccaaccttcc ccaccacatc cgcaacggac acctgaccgg cacacccaca ccctcaatct
    10081 gccacgacat cgcctcacgt cagcttgccc gccccgacac ccaagccgtc ctgaccatcc
    10141 tcagcccctc cggacacgag gattacaaaa aaacagcacg cgcccttatc cgcctgtccg
    10201 aacagaccgc caaacccctg ctcgtcagca gccccttttc agacggcata acacatttcg
    10261 acacccccac tcaggcaatc cgcacccttt cctaccgcaa caccgccgcc gccctgaaac
    10321 aggcacagct cgacattgca ccgccgcagc catgccgtct gaaaacaccg caaccccaaa
    10381 acatcaaaaa agcccttgca gcggcaaatc cctccctgct cgccgaagcc ctgcacctcc
    10441 ccccctaccg gcacactacc cataacgccg tccaattcca attcggcagc caccccctct
    10501 atggcgacat cctgaccgca tgctgcaacg gacaaaccac tgccgtactc ccgccgttta
    10561 ccacgctcga cagccgccac cttgcccgct ttgccgaact cgacggcaca caaaccctcg
    10621 accagttcct gcacacactg accgtcattc ccgaataccg ccaacacatt ctcggcatca
    10681 ccctcaacct caacggcgga caatacagca gcgatttcat cttaaagaca cccgaaacac
    10741 acgacacgcc caaacgcaag aacacaggca aagccgccca aaccctcgaa catgccgccg
    10801 caaaaatgca gagtgccgcc gcatacctga aacacaaaaa cccgacagcc gccgaatttc
    10861 tccgccacac aagcgaagcc gccgcagaac tgctcggcag caaaaccgaa accggagcag
    10921 ccgtacccaa cgtacttgcc ccctatcccg cagcacacct caaaacactg tccctaaaaa
    10981 acaacacgac cgttaccatt accccccttt tgcccgaaga cgcagaagcc aaacagcagt
    11041 tcgtccgcag cctcagcccc gaagcacggt acacacgttt catgacccac accaacgaac
    11101 tgcccgcagc cacgttggca cgcctgtgca accccgacta ccactgtgaa gccgcatgga
    11161 cggcaaggga tgccgacagc aacatcgtcg ccgtcgtccg ccacagccgc ctgaaccgca
    11221 acgaatgcga attcggcatc acactggcgg aacatatgcg cggcagcggg ctggcacaga
    11281 aaatgatgga actcatcatc caaaccgccg cacagcaagg ctaccggact atgagtgccg
    11341 acattctcaa aaccaatact cccatgatca aacttgctga aaaatcagga tttaccctca
    11401 aggaatcgga caccgaaaaa aacctgtacc gcgcatatct gaaccttgcg gcagacaaaa
    11461 caacagaaaa aacaaataaa aacttgcaca ccgaccacaa aataacctaa aatcggcagt
    11521 ttccatatat ccgattttct caaaaggact caaaatggta gttatccgtt tggcacgcgg
    11581 cggctcgaaa caccgccctt tctacaacgt catcgttact gactcacgca gccgccgcga
    11641 cggccgcttc atcgaacgcg taggcttcta caaccccgta gccaacgaaa aacaagagcg
    11701 cgtccgcctc aacgcagacc gcctgaacca ctggattgca caaggcgcgc aagtcagcga
    11761 ctccgttgca aaactgatta aagaacaaaa agccgcctaa tccacatttg ccgccatgac
    11821 agacactcaa aaccgggtag ccatgggcta catcaaaggc gtattcggca taaaaggctg
    11881 gttgaaaatt gccgccaaca ccgaatattc cgacagcctt ttggactacc ccgagtggca
    11941 tttggtcaag gacggcaaaa ccatcagcgt tacccttgaa gccggaaaag tcgtcaacag
    12001 cgaactccaa gtcaaattcg aaggcataaa cgaccgcgac ttggcattct cattgcacgg
    12061 ttacaccatc gaaatacccc gtgaagcatt cgccccgaca gaagaagacg aatactactg
    12121 gacagacttg gtcggcatga ccgtcgtcaa caaagaccat accgttttag gcaaggtaag
    12181 caacctgatg gaaaccggcg caaacgacgt attgatgatt gacggagaac acgggcagat
    12241 tctgattccg ttcgtttccc aatatatcga aaccgtcgat accggcagca agattattac
    12301 tgccgactgg ggtttggact actgatgctt atccaggcag ttaccatttt ccccgaaatg
    12361 ttcgacagca ttacccgcta cggcgtaacg ggacgcgcga acagacaggg aatctggcag
    12421 tttgaagcag tcaatccccg aaagtttgcc gacaacagat tgggctacat cgacgaccgc
    12481 ccgttcggcg gcggcccggg aatgattatg atggctccgc cgcttcatgc agcaatagaa
    12541 cacgccaaag cacaatcttc ccaagctgca aaagtcattt acctcagccc ccaaggaaaa
    12601 ccgctgacac acccaaaagc ggcagaactg gcagaacttc cgcatctgat tctgctgtgc
    12661 ggacgctatg agggaataga cgaaagactg ctgcaaacca gcgtcgatga agaaatcagc
    12721 atcggagact tcgttgtttc cggcggagag cttcccgcca tgatgctgat ggatgcggta
    12781 ttgaggctcg tacccggcgt attgggcgat atgcagtctg ccgaacaaga ttcgttctca
    12841 agcggcattt tggactgccc ccactacacc aaacccttag aatttcaagg catggctgtt
    12901 ccggaagtat tacgttccgg caatcatggc ttgatagcgg aatggcggtt ggaacaatcg
    12961 ctgcgccgca ccttggagcg cagacccgat cttttggaaa agcgcgtttt aatcccaaag
    13021 gaatcccgcc tcttagaaac catccggcaa gagcaacggg aaatccaatc ataatttagg
    13081 aaaaaaacaa tgaacctgat tcaacagctc gagcaagaag aaattgcccg tctgaacaaa
    13141 gacatccccg aattcgcacc gggcgacacc gtagtcgtat ccgtacgcgt cgtggaaggt
    13201 acccgcagcc gtctgcaagc ctacgaaggc gttgtcattg cacgccgcaa ccgtggtctg
    13261 aacagcaact tcatcgtccg taaaatttcc agcggcgaag gtgtggaacg tactttccaa
    13321 ctgtactctc cgaccgtcga aaaaatcgaa gtcaaacgcc gtggcgacgt acgccgtgcc
    13381 aaactgtact acctgcgcgg cctgaccggt aaagctgcac gcattaaaga aaaactgcct
    13441 gcacgcaaag gttgattcaa accgttttcc cccaatgccg tctgaacctt cagacggcat
    13501 tttttatcgc cgcaatatca acaagacaac gatattccca atccgcctcc cctgcttaac
    13561 aagcactccc gtctattcaa acaaaattta cagaccaaac gttatacagt atcatagccg
    13621 ccatataacg ataaccgctt tgccaacttc cctttcaaaa ccgcaaatca ttatgacacc
    13681 ttccctctta ctgtcaggat tgaccttccg actcttcctc gccctgattg ccgtatccct
    13741 tttatggggc gtttaccttt gggcggtatc ggtatgagca tcatcgttga aaacctgacc
    13801 gtcagctacc gccgccgacc tgccgtgcac catgtggaca ttacttttga agaacatagt
    13861 atgtgggcgg ttttcggtcc caacggcgcg gggaaatcca cctttctcaa atccttaatg
    13921 ggattgcagc ctatcgatac gggcagcatc cggctggacg gattgacccg tcagaacatt
    13981 gcctaccttc cccagcagtc cgacatcgac cgctcgcagc ctatgaccgt tttcgacttg
    14041 gcggcaatgg ggctatggta tgaaatcggt tttttcagag gaataaatgc cgttcaaaaa
    14101 caacgcgttc acgaatcttt ggaacgcgtc gggatgcggc agtttgccgg ccgccaaatc
    14161 gcccacctct caaacggaca attccagcgt gtcctttttg cccgaatgct ggttcaaaac
    14221 gccaaattcc tacttctaga cgaacctttc aacgctgttg atacacggac gacctacgaa
    14281 cttctcgacg tattgcagaa atgccattgc ggcggacacg ccatcatcgc cgtactgcac
    14341 gattacgaac aagtccgtgc ctactttccc aataccctgc tgctcgcccg cgaaaaaatt
    14401 gcggcaggcg caaccgagac cattctgaca gaaccctacc tcgcccaagc caacgccaaa
    14461 atgcagcaac aggaaagccc cgagtggtgc gcctcataaa tgccgtctga aaccatgtcc
    14521 ccgtaccaaa cactatgaac ccctacgacc tgctcatcac accctttgca gaattcgact
    14581 ttatgcgcta cgccctcgca tccgtcttct gcctgtccct cagtgccgca cccgtcggcg
    14641 tattcctcgt catgcgccgt atgagcctga taggcgacgc attgagccac gccgtcctgc
    14701 ccggtgccgc cgtcggctac atgtttgccg gcttaagcct gcccgccatg ggtttgggcg
    14761 gcgtagccgc aggtatgctg atggcactgc ttgccggact cgtcagccgc ttcaccaccc
    14821 tgaaagaaga tgccaacttt gccgcctttt atctcagcag cctcgccata ggtgtagtcc
    14881 tcgtcagcaa aaacggcagc agcgtcgatt tgctccacct ccttttcggc tccgtacttg
    14941 ccgtcgatat tcctgccctg caactcatcg ccgccgtatc caccctcaca ctgcttaccc
    15001 ttgccgtcat ctaccgcccg ctcgtactcg aaagcatcga ccccctgttc ctcaaatccg
    15061 tcggcggcaa aggcgggctt tggcacgtac tctttctcgt cctggtcgtc atgaacctcg
    15121 tatccggctt tcaagccctc ggcacactca tgtccgtcgg actcatgatg ctgccagcca
    15181 ttaccgcccg cctgtgggcg aagcacatgg gcgcactcat cctcctatcc gttctgacag
    15241 ccctgctgtg cggcttgagc gggctgctca tttcctacca catcgaaatc ccttccggcc
    15301 ccgccatcat cctctgttgc agcgtccttt atctcttttc cgtcatactc ggcaaagaag
    15361 gcggcattct gaccaaatgg ctcaaaaacc accgccacca caccacctga ccccgcaagc
    15421 cgcttgaaca aacaggcggc ttgttttacc ataacagatg ttacgttata tcacaggaga
    15481 acaattatga aacaccccaa actcaccctt atcgccgcat tgctgaccac tgccgcaact
    15541 gccgcccccc tgccggttgt aaccagcttc agcattttag gcgacgtagc caaacaaatc
    15601 ggcggagagc gcgtatccat acaaagtttg gtcggagcca accaagatac gcacgcctat
    15661 catatgacca gtggcgacat taaaaaaatc cgcagtgcaa aactcgtcct gctcaacggc
    15721 ttaggacttg aagctgccga tgtacaacgt gccgtcaaac agagcaaagt atcctatgcc
    15781 gaagcgacca aaggcatcca acccctcaaa gccgaagaag aaggcggaca ccatcacgac
    15841 cacgatcatg accacgatca tgaccacgaa ggacaccacc atgaccacgg cgaatatgac
    15901 ccccacgtct ggaacgaccc cgtccttatg tccgcctatg cccaaaacgt cgccgaagcc
    15961 ctgataaagg ccgaccccga aggcaaagtt tattatcaac aacgtttggg caactaccaa
    16021 atgcagctca aaaaactgca cagcgacgca caagccgcat ttaacgccgt ccctgccgcc
    16081 aaacgcaaag tcctgaccgg gcacgatgcc ttttcctaca tgggcaaacg ttaccatatc
    16141 gaattcatcg ccccgcaagg tgtgagcagc gaagccgagc cttcagccaa acaagtcgcc
    16201 gccatcatcc gacaaatcaa acgcgaaggc atcaaagccg tctttaccga aaacatcaag
    16261 gacacccgta tggttgaccg tatcgccaaa gaaaccagtg tcaacgtcag cggcaaactg
    16321 tattccgacg cactcggcaa cgcgcccgca gacacctaca tcggaatgta ccgccacaac
    16381 atcaaagcct tgaccaacgc gatgaagcaa taaaaccgtt tgattggttt gaatttgaaa
    16441 aggcaaaagg ccgtctga
//
LOCUS       DAWFBO010000030        16415 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFBO010000030 DAWFBO010000000
VERSION     DAWFBO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.030"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(245..3034)
                     /gene="ileS"
                     /locus_tag="WGO87_000707"
     CDS             complement(245..3034)
                     /gene="ileS"
                     /locus_tag="WGO87_000707"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0392096.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLASIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVADMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREKLEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(3175..4095)
                     /gene="ribF"
                     /locus_tag="WGO87_000708"
     CDS             complement(3175..4095)
                     /gene="ribF"
                     /locus_tag="WGO87_000708"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0392097.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKRW"
     gene            complement(4622..5917)
                     /gene="tyrS"
                     /locus_tag="WGO87_000709"
     CDS             complement(4622..5917)
                     /gene="tyrS"
                     /locus_tag="WGO87_000709"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0392098.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(5980..7857)
                     /locus_tag="WGO87_000710"
     CDS             complement(5980..7857)
                     /locus_tag="WGO87_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0392099.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSPRVQFGFLAALCALSLAASFMPSAFDKYYLPHLRACEMLVGSLTAVWMRYRQQRN
                     LAVGRCCAAAGALFSACILSACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPL
                     KKFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAVVLILLLSLFSY
                     HCIEKPFKKWQGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCH
                     NNTGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWAATMVSADACAYVEGY
                     ASRVFQNWAACRAVYRYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFKKFDRM
                     LHKLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQTLRPDDESTLKANARIREL
                     AAKYPNVYIIDAAAYIPANFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHN
                     H"
     gene            complement(7868..7972)
                     /locus_tag="WGO87_000711"
                     /pseudo
     CDS             complement(7868..7972)
                     /locus_tag="WGO87_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(8224..9315)
                     /gene="ychF"
                     /locus_tag="WGO87_000712"
     CDS             complement(8224..9315)
                     /gene="ychF"
                     /locus_tag="WGO87_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0392100.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9429..11105)
                     /locus_tag="WGO87_000713"
     CDS             complement(9429..11105)
                     /locus_tag="WGO87_000713"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0392101.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAVLLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSSGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            11280..11711
                     /locus_tag="WGO87_000714"
     CDS             11280..11711
                     /locus_tag="WGO87_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0392102.1"
                     /translation="MSSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGKNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(11942..12637)
                     /locus_tag="WGO87_000715"
     CDS             complement(11942..12637)
                     /locus_tag="WGO87_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0392103.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12653..13153)
                     /gene="hpaC"
                     /locus_tag="WGO87_000716"
     CDS             complement(12653..13153)
                     /gene="hpaC"
                     /locus_tag="WGO87_000716"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0392104.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(13268..13708)
                     /gene="nadR"
                     /locus_tag="WGO87_000717"
     CDS             complement(13268..13708)
                     /gene="nadR"
                     /locus_tag="WGO87_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0392105.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(14051..14560)
                     /locus_tag="WGO87_000718"
     CDS             complement(14051..14560)
                     /locus_tag="WGO87_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0392106.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14639..14714)
                     /locus_tag="WGO87_000719"
     tRNA            complement(14639..14714)
                     /locus_tag="WGO87_000719"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14679..14681),aa:Lys,seq:ttt)
     gene            14925..16004
                     /gene="apbC"
                     /locus_tag="WGO87_000720"
     CDS             14925..16004
                     /gene="apbC"
                     /locus_tag="WGO87_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0392107.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQHSDGIHIALHF
                     GFPVAHIAAAIADSVQETLMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSGGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGAAAQLFDKHPAIARIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
BASE COUNT         3733 a         4470 c         4420 g         3792 t
ORIGIN      
        1 aatctcgaaa tccgtcattc ccgcacaggc gggaatccag acccctgacg cggcgggaat
       61 ctatcggaaa tgactgaaac ctcgagattc tagattccca ctttcgtggg aatgacggtt
      121 cagttgcatt ccgacaacac cgcaatcttg aaacccctcc gccgttataa agacaaatcg
      181 cggcacaaaa aatgccgtct gaaatgctgt tcggcggttt cagacggcat ttgctcaaac
      241 tttatcaggc gtaatggcgc gtttcgcctt ctccgccgac attctctgca cagcgtttgc
      301 agacggtttc atagcctgca accgcgccca catcgcgggt gtagtgccag cagcgttcgc
      361 atttttcacc atcactggct ttagcggcaa cggcaagttc gctgcctact ttcacttctg
      421 ctttagacac cagcaaagca aagcgcaatt cttcgcccaa agcattcaga tagccggcca
      481 tttcttccgg cgcggtaatt tcggcttcgg cttgcaagga cgaaccgacg gttttgtcgg
      541 cgcgcaaagg ctcgatggcg gcggttaccg cttcgcgggc ttcgcggatt gccgtccatt
      601 ttttcaccag ttcggcttcg gttttttcgt tgatggtcgg gaactcgtgc caagtatgga
      661 agaggacgct gtcttcttcg ccgccgccga tgatgtccca cgcttcttcg ccggtgaagc
      721 acaaaatcgg tgcaatcaag agaaccaaac tgcgtgtgat gtgatacagg gcagtttgtg
      781 cgctacggcg tgcatggctg tcggctttgg tggtgtagag gcggtctttc aggatgtcga
      841 ggtagaacgc acccaagtct tccgagcaga aagaaacaat gtcttttacg gcaaagtgga
      901 aggcataacg cggatagtaa tcgcctgcca gacgctcttg cagctgacgt gccaatacca
      961 cggcgtagcg gtcgatttcc accatatccg cctgttgcac ggcatcttca atcggattaa
     1021 agtcgctcaa gttggcaaac aaaaagctca aggtattgcg gatacggcgg tagctttcgg
     1081 ttacgcgttt gaggatttct ttggaaatcg ccaattcgcc gctgtaatcg gtagatgccg
     1141 cccacaggcg caggatgtct gcgccgaatt cgttataaac ctcttgcggt gcaacgacgt
     1201 tgccgatgga tttcgacatt tttttgcctt cgccgtcgac aacgaaacca tgggtcagca
     1261 gctgtttata cggcgcgcga cccattgatg aggcgcagcc ggtcagcatg gacgattgaa
     1321 accagccgcg gtgttggtcg ctgccttcga gatacaaatc agccggccat tccaattttt
     1381 cgcgttgttt cacaacggaa taatgggtcg agccggagtc gaaccatacg tccattgtgt
     1441 cagaaagttt atcgtaattt tcgcaatctt ccgcgctcaa gagttcgctc ttatcgaggg
     1501 agaaccacgc ttcgatgcct ttttcttcga ttttcagggc aactttttcc aaaagttcgg
     1561 cagagttcgg atgcagctcg cccgtttctt tgtgaacaaa gaaagtcatc ggcgtgcccc
     1621 aatagcgttg gcgtgaaacc acccagtcag gacgaccttc aatcatggct tccaaacgcg
     1681 cgcgacccca agacgggaag aattcggtgt cgtccacggc cttgatggct ttgtcgcgca
     1741 gggttttgcc gtcggcacca gctttgtcca taccgacaaa ccattgacct gtcgcgcggt
     1801 aaatcagcgg cgttttgtgc cgccagcagt gggcgtagct gtgttcgatt ttactgcttg
     1861 ccaaaaggtt gccggtttct tccaaccatt gcaggatgac ggggttcgcc tcccaaacgc
     1921 gcatatcggc aacgcgcggc gtttcgccga tgtatttgcc ttcggcgttg acaggattgt
     1981 aaagctcgat gccgtatttg ttgcagacgg cgtagtcttc caaaccgtgc gcgggcgcag
     2041 tatgcaccaa gccggtaccg gcatcggtgg taacgtgttc gccgttaagc atgggaatat
     2101 cgcgttcgag gaacggatgg ttcatgtgca ggttttccag cttgtcgccg gtggtttcgg
     2161 caaggatggc gatgccgtct gaaaagccgt aacgtttgag cgcgtcttcc gccaaatctt
     2221 tcgccaatac caatttgcct ttgggcgtat caatcagttg atacaccacg tccgcgccgg
     2281 cagatacggc ttggctcgca ggcagcgtcc aaggcgtagt cgtccagatg acggcaaacg
     2341 ctttgccttc gatacttgcc aaaccgaatg cggcggcaag cgcggcagtg tctttaaacg
     2401 gataggcaac gtcaatcgcg ggcgagattt tgtctttata ttccacttcc gcttcggcca
     2461 gcgaagaacc gcagtccaag cagaattgaa ccggtttcgc accccggtag agatagccgg
     2521 atttgtagat ttcgccgagc atacgcacgg tatcggcttc ggttttgaaa tccatagtca
     2581 ggtaaggatg gtcccagtcg cccaacacgc ccaagcggat aaagtctttt ttctgacggg
     2641 caatctgttc ggcggcgtat tcgcggcaca attcgcggaa gcgggctttg ggcatgtctt
     2701 tgccgtgcag cttttccacc atcacttcga tgggcagacc gtggcagtcc caacccggca
     2761 cataaggcgc atcgaaaccg gcttgggttt tgctgcggat aatgatgtct ttaaggattt
     2821 tattgacggc gtgaccgatg tggatgtcgc cgttggcata cggaggaccg tcgtgcagaa
     2881 tgaatttcgg gcggcctttg gcgatttcgc gcagtttttg gtagcgtttt tgctcgtacc
     2941 agcttttcag ccatgcaggc tcgcgcttgg caagattgcc gcgcattgga aacgggctct
     3001 cgagcaggtt gacggttttg ctgtaatcgg tcatttttta atctctattg ttacaatatt
     3061 tcggttcaga cggcattgcg cctcaaacag tatttcacaa cgggaaaccc ctatgccgtc
     3121 tgaaaagtta aaagattgat tgtagcccaa tcggatggtt tgtataaggt ttttctacca
     3181 acgctttgcg gcttccatat cggcttcaat ctgccttttc agttcttcca taccgtcaaa
     3241 cttttcctca tcgcgcagtt tgtgcaggaa gcggacgttc agccgttgtc cgtacaggtc
     3301 gccttgaaag tcgaacaggt ggacttcaag cttttgagaa cagccgctat caacggtggg
     3361 attgaagccg aagctcgcca cgccgcgccg cgtgccgaat gcgccgtccg cttcgacgac
     3421 gaacacgccg ccgagtgcat aacggtggcg gggcaggcgg atgttggcag tcggggcgtt
     3481 taaggtgcgt ccgagttttc tgccgtgcac caccctgccg ctcaagacgt agtcgtgtcc
     3541 caaaagtttt ttcgcatagg caaggttgcc gtctgaaagg gcttgtcgca cggcggtact
     3601 gctggtgcgg atgtcttcga cgatgacgga aggcgtacgc tcggtctgca tatcgggctg
     3661 ttgtgccaaa agttcaaaac agccttcccg ccccgcaccg aaacggaaat catcgccgac
     3721 gagcaaataa cgcgtattca aggtttgacg cagcaggcgg tcgataaacc cttgcgcgga
     3781 tatttcggaa aaattttgat cgaaacgcaa aacccagacg gcatcgacac agcctgtgcc
     3841 ttccaataat tcgagcttgg tgcgcagggg gctgatccga cacggtggca tcctgccggt
     3901 gcggagtgcg aaaaattctt tgggttgggg ttcgaaaacg acggtcacga cgggtaatcc
     3961 gcgcgtatcg gcttcgaggc ggagtttttg gaggatgtgt ttgtgtccga ggtgtacgcc
     4021 gtcaaaattg cctatggtta cggcggcccc gtgcggaaag tcgggcgcgt tgtgccgccc
     4081 cagcctgatt ctcattgtta tgttcgggta tgtggtgaaa caggcggtca ttgtaaacgg
     4141 tattgcggtt tatagacagt gtgtcgcccg ttacgcccgc ccgacgcggg aaaagtaggc
     4201 aaatttcccg ccgccgaacg cgccaaacgc ataaagcgcg cagcaggcgc ggtgctatgt
     4261 gttgaaacat cgccccaaac ctgatgcaac atcagtgaaa atcgttcttt tttaaccagg
     4321 taaaccgaga caaacaaccc tccgccgtca ttcccgcgca ggcgggaatc cggacctgtc
     4381 cgcacagaaa cttatcggat aaaaacagtt gctcaaaccc cgagattcta gattcccact
     4441 ttcgtgggaa tgacggttca gttgcgttcc gacaacaccg taatcttgaa actcgtcatt
     4501 cccgctcagg cgggaatcta gaacgtggaa tctaagaaac cgttttgccc gataagtttc
     4561 cgtgcggaca ggtccggatt cccgcctgcg cgggaatgac ggcatttctg cggcaatcgg
     4621 attatttcca aaccaaaagc gcgtggttgc gtttgccgcg ccgaaggatg gtgtatttgc
     4681 cgaaacgttt gtgttcgtcg gtcagcaggc aggcatcgtc ggggcgttcg gcggcgtggt
     4741 tggggttgtt ggcttcggca ggtttgccgt tgagcaaaac cgctttgctg ttcacaaagc
     4801 cgcgcgcttc tttattggag gatgccaaac cggttttgac caaggcttcg acgacattga
     4861 tgccgtctga aacttcaaat gcgggcaggc cgtcgagtgc cagctgctcg aagtcgcttt
     4921 cggtcaggct gctttggtct tcggcaaaca ggctttcgga aatgcgctgc gcggcggcaa
     4981 gggcttcttc gccgtgaatc aggcgggtca tttcttcggc gaggatgcgt tgcgcttcgg
     5041 gcttgctgcc gcttgcctgg tctttggctt cgacggcatc gatttcttcg atggacagga
     5101 aggtaaagta tttcaggaat ttatacacat cggcatcggc gactttcagc cagaattggt
     5161 agaactgata gggcgaggtt tttttcgcgt tcagccatac tgcgccgcct tcggttttgc
     5221 cgaatttggt gccgtctgat ttggtaacca aaggcagggt cagtccgaac acttgttttt
     5281 ggtacaggcg gcgggtcagg tcgataccgg cagtgatgtt accccattgg tcggagccgc
     5341 cgatttccaa aaccgcgccg tggcgtttgt tcaactcggc gaagtcgtaa ccttgcagca
     5401 gggaataggc gaactcggtg aaggaaatgc ctgcgccgtc gcggtcgatg cgctgtttga
     5461 cggattcttt gttcagcatg gcgttgacgg agaaatgctt gccgatgtcg cgcaggaagt
     5521 cgaggcagtt cattttgccg aaccagtcgg cgttgttcgc cataatggcg gcgtttccgc
     5581 cttcaaagct caagaaaggg gttaattggc tgcggatgct ttccacccag ccggcaacgg
     5641 tttcggcgga attcaagctg cgttcggcgg ctttgaagct ggggtcgccg atcataccgg
     5701 tcgcaccgcc caccagtgca atcggcgtat gccccgcctg ttggaagcgg cgcaatgcca
     5761 atacgggcag caggtgtccg atgtgcaggc tgtcggcggt cgggtcgaag ccgcaataaa
     5821 gggaaatttt ttgttcgttc aacaaagcgt ctaaggcttc gatgtcggtg gtttgcgcga
     5881 ttaggccgcg cgattgcagg tcttggatga cgctcatcgg tctctttcaa aaaaaattag
     5941 cggttttgca aaccgccgat tgtaacaaat ttaagcgaat caatggttat ggcgcgtatc
     6001 gagaaaccgt tgtttttcgg aaaaacgctt cgccaattcc gtgccgccgt aagggttgat
     6061 gtggtctttg tccgagtaaa ccggcaatcc gccgatttgg aaatttgcgg ggatataggc
     6121 ggcggcatca ataatataga cgttggggta tttggctgcc aattccctga tgcgtgcatt
     6181 ggctttcaga gtgctttcgt cgtccgggcg cagggtttgg cggtaacccg gtatgcgtga
     6241 agacaagata taggcgcgtt gtacgttgta agacgaggca aggttgtccg ccatcaggta
     6301 aacggcttgt ttttcggacg agagtttatg cagcatacgg tcgaattttt tgaaaaaacc
     6361 ggcatcgtag gcaagggagc ggctgttttc gggcatttgg ctgccccacc gcatcgccaa
     6421 aaccactttt gaataccggg gcaggtgttc ttcggcatag cggtaaacgg cgcggcaggc
     6481 ggcccagttt tggaatacgc gggacgcgta gccttccaca taggcgcagg cgtcggcgga
     6541 aaccatagtg gcagcccatt tttctttttt gcccacggca tcgaagaatg ttttgtaatg
     6601 gtcggcgtgg gagtcgccca aaaccagcag ttccggctgt ttttccgtat ccccccatag
     6661 gcattgtttg ccggtattgt tgtggcagga ggtgttggaa cgcgtcagcc ccaagcggtc
     6721 gtattgcgcc ataaacggca gcctcatcgc aaaaaatgag cccgccccca aaatgagcat
     6781 aggcaaggca taaatccata aaacggattg tgcgaacgaa ccttgccatt ttttaaacgg
     6841 tttttcgatg cagtggtaag aaaacaggga aagcagcaat atcaggacga ccgccgccgc
     6901 cggcgaataa ggcggcaggt tgtccgggcc gatatagcgc ataaaggcca atatcggcca
     6961 atgccacaga taaagcgaat aggaaatcaa accggcggca acggtgattt tccattggaa
     7021 aaatttttta agcgggtgtt cgtaatgatt gaaataaatc agcgcggcaa cagccagaca
     7081 gggaatcaaa gcggcgggcc ctgggaaata ggcggtttgt tccgaatagg caaacaggca
     7141 ggcggacagt atgcacgcgg aaaacaatgc gccggcggcg gcacagcatc tgccgacggc
     7201 gagattccgc tgttgccggt accgcatcca cacggcggtc agcgatccga ccagcatttc
     7261 gcaggcgcgc aggtggggca ggtaatattt atcgaacgcg gaaggcataa aggaagcggc
     7321 aaggcttaag gcgcacaatg cggcgaggaa gccgaactgt acgcgcgggc ttttgcgggc
     7381 gacaagcagc agcaatatcg gaaagacaaa gtaaaattgt tcttcgaccg acaaagacca
     7441 aatgtgcagc aagggctttt cttcctgcgc gggatcgaaa taatccttcc cccttgcaaa
     7501 atacaggttg gaggcgaaac ccaaggcggt cagcgcggat ttccacaaaa gaaagaaatc
     7561 atctttggtg aataaaaaga agccgcctgc cagcgttgcc gccaatacgg cgaaaaatgc
     7621 gggcaaaatc cgtttgatgc ggcggatata aaatgccttc agggaaaacc tccccccccc
     7681 cgacatttcg cgaaggagga tcgccgtcat caaaaagcct gaaatcacaa agaatatatc
     7741 gacaccgaga aatccgcccg gcagccaatt tttttcgata tggaacacga tgacggacaa
     7801 aacggcggcg gcgcgcaatg tgtcgatgtc cgggcggtag ggtaaggctt ggctcataat
     7861 gtttttatag tggattaaat ttaaaccagt acggcgttgc ctcgccttgc cgtactatct
     7921 gtactgtctg cggtttcgtc gccttgtcct gatttaaagt taatccacta tactcgaaac
     7981 gcggcggcgc aaatgccgtc tgaaaggtca ttttgtacgg gatattcgga atgccggacg
     8041 gctttttgca acagcggggc aggcggtttg ttttgcagga atcggggagg gcaaatcgga
     8101 aatgcgggtg ggattttatt ttgatgcagc cgcattccgg cggtatggaa acgccgaaaa
     8161 ccaccaaaac cggcttcaga cggcatttct ggcaaagccg tctgaaaacc tgccgcattt
     8221 gggttacacg ttaaacaaaa agtgcatcac atccccatcc tgcacgacgt attctttgcc
     8281 ttccacacgc attttgccgg cttctttggc tttggcttcg ccgccgagcg agacaaaatc
     8341 atcgtaggca atcacttggg cgcggatgaa gccgcgttca aaatccgtat gaatcacgcc
     8401 ggcggcttgc ggggcggtgt cgcctttgtg tatcgtccac gcgcggactt ctttcacgcc
     8461 ggcggtgaag taggtttgca ggcccaagag gtcgtagccg gcgcggatca ggcggttcag
     8521 gccgggttct tccaaaccca tttcggcgag gaattcggct ttttcgtcgt cttccaattc
     8581 ggcgatttcg ctttccatcg cggcgcatac ggcgacgacg ggggcgtttt cttttgccgc
     8641 caattctttc aggcggtcga ggtgcggatt gttttcaaaa ccgtcttcgg cgacgttgcc
     8701 cacatacatc gcaggtttgg ctgtcagcag gaacagcggt ttgagcatcg cgcgttcttc
     8761 cgcgtccaaa ccgaaggaac gcacgggttt gccttcgtcc agatgcggca gcagtttttt
     8821 gcacaaatcg accagctttt gcgcgtcttt gtcgcctgaa cgggcgcgtt tttcttcgcg
     8881 gacgatggct ttttccacac ttgccaggtc ggcaagtgcc aactctgtgc cgatggtttc
     8941 gatgtcggca atcggatcga cgcggcctgc aacgtggacg atgttgtcgt cgtcaaagca
     9001 gcgcacgaca ttcacaatcg catcggtttc gcggatgttg gcaaggaact ggttgcccaa
     9061 gccctcgcct ttgctcgcgc ctgcaaccaa accggcaata tcgacaaatt cgacgatggc
     9121 aggctgcatt ttttgcggat tgacgatttt tgccaattcg gccatacgcg gatcggggac
     9181 ttcgacgatg ccgacgttgg gttcgatggt acagaaagga tagtttgccg cttcgatacc
     9241 cgattgggtc agcgcgttaa aaagggtgga tttgccgacg ttgggcaaac cgacgatgcc
     9301 gcatttcaaa ctcatgtttt ttcctgaaaa tagagaaatt taacggcgga ttatagcata
     9361 ccgccgcccg cgttccgaaa aaatgccgtc tgaaacggct tcagacggca tctggtttca
     9421 aaaaccgttc agaacaagcc gtgaatcacg ccttctgcgt ccacgtcgat tttctcggcg
     9481 gcgggaactt tgggcagacc cggcattttc atcatattgc cgcacaacgc aacgatgaaa
     9541 ccagcaccgg aagaaacggt aatgccgcgc acggtaatgc ggaagccttc agggcagccc
     9601 aagagtttgg cgttgtcgct caaagaatat tgggttttcg ccatgcaaat cggcattttg
     9661 tccaagccca gtttttccag cgaggcgatt tcggcagacg cttccgcgct gaaatcaaca
     9721 tcttccgcgc cgtacacttt ttgggcaatc gcacggattt tgtctttaat actcagctca
     9781 acatcgtagg aaaaattgaa gttgttcggt tggttttcga tggcgttgac gactttgcgc
     9841 gccaagtctg caccgcccac gccgcctttg ccccacactt cggtcaagga aacttcaacg
     9901 ccgtgttcgg cacaggcttt ttcaatcatc gccaactcgg catcggagtc ggacacgaag
     9961 cggttgagcg caacgacgac gggcagtccg aatacgtttt tcaggttgga aatgtgtttc
    10021 agcaggttgg gcaaaccttt tgccaaggct tcgaggtttt cttcaccgag gttggcacgt
    10081 tccacgccgc cgttgtattt caaagcgcgg acagtcgcca cgacgaccgc cgcatcaggt
    10141 ttcaaaccgg caaggcggca tttgatgtcg cagaattttt ccgcgcccaa atccgcgccg
    10201 aagcctgctt cggttacggc gtaatcggca aggtgtttcg ccagacgggt tgcggttacg
    10261 gagttgcagc cgtgggcgat gttggcgaac gggccgccgt gtacgaaggc gggcgtgcct
    10321 tcgatggttt gcaccaaatt gggcttaatc gcatctttga gcaggaccgc catcgcgccg
    10381 tgtgccttca aatctttggc gtaaacgggg ctgccgtctt tggcgtaggc gacaaggatg
    10441 ttgcccaaac gctctttcaa atcgctgatg tctttggcaa ggcagaatac cgccatcact
    10501 tcggaagcaa cggtaatgtc gaaaccgtca ggacgcatca cgccgtcaac aggtttaccc
    10561 atgccgtcga tgatgttgcg caactggcgg tcgttcatat cgaccacgcg ccgccacagc
    10621 acgcgtttcg gatcgatgtt caactcgttg ccttggtaga tatggttatc gagcatggcg
    10681 gcgagcaggt tattcgccgc accgatggcg tggaagtcgc cggtaaagtg cagattgatg
    10741 tcttccatcg gcaaaacttg ggcatagccg ccgcctgccg cgccgccttt cacgccgaac
    10801 accggcccca gagaaggttc gcgcagggca atcacggcat ctttgccgat gtggcgcaac
    10861 gcgtccgcca aaccgatggt tacggtggtt ttgccttcgc ccgccggagt cgggttgatg
    10921 gcggtaacca aaatcagcct gccctgtttt tgcggcagtt tgaacgcttc ggcaggattg
    10981 attttcgcct tgtaatgacc gtaaggctca atgttgtcaa cgttcagacc cagcttggcg
    11041 gcaatttcgc caatcgggcg catcgtggag gattgggcga tttcggcatc ggttttgaag
    11101 ctcatgattt tcctttagaa atgaggaggg acataccgtc tgaaagcatc aggcgacaaa
    11161 caggtggatt gaaaataata tcaggcatat tataacgtta tccgcaccaa acccgcagtg
    11221 aaatttttga cgcagcaaca aaaataccgt tcatattgtt cacaatccaa ggagaaaaca
    11281 tgagcagcaa cgcatggctg ttttgggcat tggcatcggc aggcttcgcc tcattgaccg
    11341 ccatcttcgc caaaatgggt ttacagggta tagattcaga tttcgctacc ttcatccgca
    11401 ccttggtcat cctcgccgct ttgttattgt ttttgaccta caccggcaaa tggcagggcg
    11461 tgaacggatt tacaggaaaa aactggacat tcttaattct gtcgggtttg gcgaccggcg
    11521 catcctggct cgcctacttc aaagccctgc aactgggcaa cgcctcgcaa gtcgcgcccg
    11581 tcgacaaatt cagcctggtc ctggtcgccc tgatggcggt ggtctttttg gacgaacgcc
    11641 cgaacacgca ggaatggata ggcttgggac tggtaacggc gggcgtgttg gtgctggcgt
    11701 tgaaacgtta aaccgaatcc gccataccgc ctgaaaccgg gttttgacaa tctgacgggc
    11761 ggcggtctga acaaagttac ccaataaaat ccgacaccgc gccgacggca aaccgccgcg
    11821 cttcagatgg catttgttgg acggaaagcc ccgaacgccc tattttcacg cctccacgaa
    11881 aatatgccgg acatccggtc ggaaccgttc ggcagccggc tcggcaagcc accgttttat
    11941 ttcacttcca agcccctgca agggcttgag cctctttcag acggcatacc gtgccgacat
    12001 ccagccacaa gcccgtatgc ttctgaccgc tcacgcggtt ttgccgcatt tcgccacgca
    12061 atacgggcgc gagtttcgcc acactgcccg cttcgattcc gtcaaacatt tcaggacggt
    12121 aaatacccac gccgctgaat gtcaatccgt tgccgccatt tacttccggc cgcacgctgc
    12181 cgtcgggcag cagggaaaaa tcgccgtcgg ggttgtgcgg cggattttcc accagccaca
    12241 gatgggcgga aatatgttcc ggcagggacg atgccgcctg aaacgcggcg gtaaaatcga
    12301 tgtcggtcag cacgtcgccg ttgaccacca aaaacggctg cccacccaac agcggcaatg
    12361 cctgcgcgat gccgcctgcc gtttccaaac cgcctgcggg ttcgggcgaa taggcgatgt
    12421 tcacgccata agccgagccg tcgcccaaag catcttctat ctgccggccc agccaagcgt
    12481 ggttgatgac gatttcggta aaccccgcct gcttcagacg gcataggtgc caaccgatta
    12541 gaggcttacc cgccacatcg agcagcggct tcggagtggt atcggtcaaa gggcgcatac
    12601 gctcgccgcg tcctgccgcc agtatcatcg ctttcatata tctgtccgaa tatcagtcta
    12661 aaaatctaaa ctgccgtctg aaatacagca gcgcggggcg tttgcacccg cagtttttga
    12721 tttcgtcgag cctgacgtaa aacacaaaat gcgtgccgat ttcatgtttg ccgacaatat
    12781 gcccgtgcag gtgcgccaac gcgccctcta tttcaagttg tcccgttttg ccgcgatgcc
    12841 agatgtggta ggcaaaccgc tcttcgggcg acagcccggt cagcccggca aaatgttcgg
    12901 caacatcctg atgttcgtcc gccagcgtat tgatgcagag gctgccgttt tccgacagga
    12961 tcggaatgat tcgcgcactc cggttgatgc acagcatcac ggtcggcggc tcgtccgtaa
    13021 ccggtgtaac cgccgtcatt gtaatgccgt aacgccctgc cgcaccgtct gtcgtgatga
    13081 catgaacgcc tgccgcacaa gatgccatcg catcacggaa cgaagtttga aaagttttct
    13141 gcaaatccgc catttttccc ctttaaactg tcccctatat aagaatgctg cacacaaggc
    13201 accccccatg tgcagcagtt ttgattcaaa aagccgtcgg tcggacgttt ccgcgcgtta
    13261 cggcgtatta cgagttcaac gcatcctcga ttttggcaag ttctgccaac aggtctttaa
    13321 gcagcagcat tttctcgcgg cccagcactt cctcgatagc gtcgtaacgc tcgtccactt
    13381 cttcgccgat ttcctcatac agcttctcgc cctcggcagt cagcttcaga aaaacgcggc
    13441 gttggtcgtt ggaaggtttc aggcggacaa ccaaacccgc tttttcaagg cgggtcagga
    13501 taccggtcag gctggggcgc agaatacacg cctgattcgc caaatcttga aagtccagcg
    13561 tgccgttttc cgccaaaaga cggataatcc gccattgctg atcggtaata ttcgcctgat
    13621 tcagaatagg cctgaattgg gtcatcaggg cttcccttgc ctgtatcaga ctgatattga
    13681 tagacgcatg ttttgattgg gtaggcattg ttcaagtctc caagttatcg aaaatcaaac
    13741 tttcaaaccg tcgggaaagc ctgtgggcgt aaattttgat gcaaccgtta tataacaaaa
    13801 cgaacatata gcaacaatac gctataaacc gcatcggacg actgggtata aaagacttta
    13861 attccgataa tcctatctaa aaatatttta atagttatat cttagtctat tttccctaca
    13921 atctcaacaa agtattacat ttactgtcga aagaactttc ttccagaaaa aacaaaagcc
    13981 gccgcatcag acgagcaagg tatatgccgt ttgattctct acatagcgta aagtttaaca
    14041 aaaacaaaag ttaaccgaaa acatccgcct gaaaaattcg tgcgcgcaag ccccaataac
    14101 tgctgattcc cgtcgtatag tgaaccattt tcccattttt gaccaaaacg acggcaggcg
    14161 ttgcgacaat ccgccaagac cttgccaaac ccccgtcctc atcgttgacc gtcggaaagc
    14221 ccaagccccg ctttgccata tacgccgcca cttcggacgc gctgccggaa cgtaccgcca
    14281 cgccgacgac cggcacgccg tccgccgcca aatcatcgat tatcggcgac tgataacggc
    14341 acacgccgca ccagctcccc caaaaataca ccaaaaccgc cttatcccgg ctaaactgtc
    14401 ccaaagtcag ccgctgcccc gacagcaggg tcaaaggccg ccctgccgca ccggccggct
    14461 cttcgggctt gcgtacccaa tccaaaaaca gcgacaccaa taaaaacacc aatgccgtct
    14521 gaacggcaaa tttgatgccc gaaagcagtt tctttttcat acgctctctc aaacggtacg
    14581 cccgcgcaac cggcaggcaa acaaaaagcc aagtctcaaa acttggcttc cggttatctg
    14641 gtgggtcgtg agcgattcga acgctcgacc aacggattaa aagtccgctg ctctaccgac
    14701 tgagctaacg acccgataag ccgtgcatta tacagcacca tcctacctcg tcaagcaaat
    14761 tttacaggct taattgcaga ccactgtttg cacgggatat tttgacaacg gattttcaca
    14821 atccgccgca taccgtgtaa aagttcgcac aaggaaaagc aaaccgcccg aaatcaatgt
    14881 acactttccg cccgtttccc aaactgcaca cagaaacaca cattatgaac atacaaaaca
    14941 tccgcaccct cctcgacacc gtcgccgttc cgaatacggc acgcacgctc ggcggcgaaa
    15001 aggccgtccg ttcggtcgaa cagcattcag acggcatcca tatcgccctg catttcggct
    15061 tccctgtcgc gcacatcgcc gccgccatcg ccgattccgt acaagaaacc ctgatgcccg
    15121 aaacaggcga cacacacatc catctgtcca tggacactga aatcggcaca cacaaagtcc
    15181 agcccggcgt taccaccatc aaaggcgtga aaaacatcat cgccgtcgca tcgggaaaag
    15241 gcggcgtggg caaatcaaca accaccgcca accttgccgc cgcaatggcg cgcatgggcg
    15301 cgcgcgtcgg cgtgctcgat gccgaccttt acggcccgag ccaaccgacc atgttgggtg
    15361 tggacgaccg caaacccgat cagaaaaacc aaaaactcat tcccgtcgaa tcttcaggcg
    15421 gcatacaggt tatgtccatc ggctttctag tcgataccgg ccaagccgtc gtctggcgcg
    15481 ggccgatggt cagccaagcc ttgcagcaac tgatgttcca aagcgagtgg gacgaagtgg
    15541 actatctttt catcgacctg ccccccggca cgggcgacat ccaactcacg ctgtcccagc
    15601 gcatccccgt taccgggtcc gtcatcgtaa ccacgccgca ggacatcgcc ctgatagacg
    15661 cgcgcaaagc cgtggatatg ttccgcaaag tcaacattcc cattttgggc gtattggaaa
    15721 atatgtccgt ccacatctgc tccaactgcg ggcatagcga agcactgttc ggcacggacg
    15781 gcggcaaaga tttggcggca cgcctcaacg tccccctgct cggacagctt cccctaagcc
    15841 tgcccgtgcg cgaagccatg gacggagggg cggcggcaca actgttcgac aagcaccccg
    15901 ccatcgcccg aatctacacc gatgccgcat tccaaatcgc cctgagcatt gccgacaaag
    15961 gcaaagactt cagcagccgg ttccctaaaa ttgtcgtcga ataaagccgc gtccgaaacc
    16021 gcaacagcaa tgccgtccca agccccgcgc ctgccgatat gccgtcattc ccgcgcaggc
    16081 gggaatccag gtctgtcggc gcagaaactt atcggataaa acggtttctt gagattttac
    16141 gttctagatt cccactttcg tgggaatgac ggaatgtagg tttgcgggaa tgacggttta
    16201 ggtattttta tagcaagccg tagggtggga tgccgccgga gggcatcata ttcttggaat
    16261 agcgcattaa aaagagtatg ataatggaaa cacaaaagat tccggcaatg cccgtgcgcc
    16321 gttcccgtcc gtcattcccg cgcaggcggg aatccagacc tgtcggaacg gaaacttatc
    16381 ggataaacgg tttctctaga ttccgcatcc tagat
//
LOCUS       DAWFBO010000031        15315 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFBO010000031 DAWFBO010000000
VERSION     DAWFBO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.031"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            465..1244
                     /locus_tag="WGO87_000721"
     CDS             465..1244
                     /locus_tag="WGO87_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0392108.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(1455..2867)
                     /locus_tag="WGO87_000722"
     CDS             complement(1455..2867)
                     /locus_tag="WGO87_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0392109.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLIAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGAINYIGTFERVYSEVSALLDSGKASFDWCNTEAVRFFP
                     ALGSLHAYKIKAELVRHYGADCYVSAKNLLKTFTTVRFEKGSDGMLDAKTAALKNGVA
                     DNPPTGLFVNKKAARRALSLWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            2958..3034
                     /locus_tag="WGO87_000723"
     tRNA            2958..3034
                     /locus_tag="WGO87_000723"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2992..2994,aa:Arg,seq:acg)
     gene            3052..3126
                     /locus_tag="WGO87_000724"
     tRNA            3052..3126
                     /locus_tag="WGO87_000724"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3085..3087,aa:Glu,seq:ttc)
     gene            3724..4899
                     /locus_tag="WGO87_000725"
     CDS             3724..4899
                     /locus_tag="WGO87_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0392110.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKYVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATVGR"
     gene            complement(4959..5507)
                     /locus_tag="WGO87_000726"
     CDS             complement(4959..5507)
                     /locus_tag="WGO87_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0392111.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(5510..7006)
                     /gene="ccoG"
                     /locus_tag="WGO87_000727"
     CDS             complement(5510..7006)
                     /gene="ccoG"
                     /locus_tag="WGO87_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0392112.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            7534..9513
                     /gene="tkt"
                     /locus_tag="WGO87_000728"
     CDS             7534..9513
                     /gene="tkt"
                     /locus_tag="WGO87_000728"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0392113.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEAEWNELFAQYQAKYPTEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKALPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAIKAAD
                     HPSCLIFSRQNLKFQARNEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     VLAEQGIAVRVVSMPSTNVFDRQDAAYQTAVLPEGLPRISVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            9654..11042
                     /gene="fumC"
                     /locus_tag="WGO87_000729"
     CDS             9654..11042
                     /gene="fumC"
                     /locus_tag="WGO87_000729"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0392114.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(11107..12030)
                     /locus_tag="WGO87_000730"
     CDS             complement(11107..12030)
                     /locus_tag="WGO87_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0392115.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALIVVGGAVTAAVGDRLFKRR"
     gene            12202..12342
                     /locus_tag="WGO87_000731"
     CDS             12202..12342
                     /locus_tag="WGO87_000731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392116.1"
                     /translation="MPSENSGDVRQASLPQNARTAQSQRSAQRKGRQQAGSDAKKRNP
                     AP"
     gene            complement(12402..12926)
                     /locus_tag="WGO87_000732"
     CDS             complement(12402..12926)
                     /locus_tag="WGO87_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0392117.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(12929..14311)
                     /locus_tag="WGO87_000733"
     CDS             complement(12929..14311)
                     /locus_tag="WGO87_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0392118.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(14422..15045)
                     /locus_tag="WGO87_000734"
     CDS             complement(14422..15045)
                     /locus_tag="WGO87_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0392119.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
BASE COUNT         3888 a         4416 c         3624 g         3387 t
ORIGIN      
        1 tataacgaat tttaattggg attatgtaaa actgtatata atcaattcag atttccaaaa
       61 aattgttttt tattataaag gtaagatagt ttttgaggaa ttaatagaac tagatggaga
      121 agggaatgtt ttacctcaat atctatttga ttcagatttg gaaaatattg aatattacga
      181 atgtgattac aagaatggca agatgcggct tttaaaaaaa gaaaaatcac attttttgat
      241 ggatattttt attattataa acctataaat tgcaggccga aacttttgta aaaccgtcca
      301 tactcattac cgcctagtcg gtaatgcatt ttcttagtag tatttgaaaa agtatgatga
      361 taaaaacggt caagcctaac tttttcataa aatccagcag agcaaggcag aaaaaaacac
      421 ccaaattggc tataatcccg acaaacacac tcaaggacaa caacatggca gcctcgcccg
      481 aagcaaaatt cactgaagaa aagattttgt gggtcaaaca ccacacgccg aaactcatca
      541 ctttcgccat cagccgtccc gaatcctacc gttttaaagc cggacagttc tcccgactcg
      601 gtttctacga aggggaaggc tttatttggc gtgcctactc catcgtttcc gcagaatatg
      661 ccgacacgct cgaatatttt gccgtactca tccaagacgg ccccatgtcg gcccgtttcg
      721 ccaaaatgca acagggcgac accatcctgc tcgataaaaa tgccaccggc ttcctcctgc
      781 ccgaacgctt ccccgacggc aaggatttgg tgatgctctg caccggctcc ggcatcgccc
      841 ccttcctttc cattctcgaa caacccgaaa tccggcagcg tttcgatacc gtcaacctga
      901 tacattccgt atcttttccc gaagaattga ttttcaacga ccggctcgcc gcattgtccg
      961 aacatcccct ggtaggcgaa tacagacact cgttccattt cgtccctgtt accacccgtg
     1021 ccgccaaccc ctcgggctta agcggaaaac gcattccgga actcttaaaa aacagcagca
     1081 tcgaacaggc gctgcatacc aagttcaccc cggaatccac acggtttatg atttgcggca
     1141 acccggaaat ggtcaaagat actttccaaa cgctgcttga catgggttac gccatgcacc
     1201 gcaaccgcat tcccggtcaa atcatgatgg aaaacggctt ctaaaaacca ccctgcttgt
     1261 ccgatgcctt cggatggacg gtcaaaccga cacggcacga aaaccgcgtc ggcaaaaatg
     1321 ccgtctgaaa aaattcagac ggcatcttcg gacacattac ctgcaaacgg caacacattc
     1381 caacccaaac cgattaggca atcaatacga acggctgttt acatacttac cggctttcat
     1441 caaccggtat cgatttaacc gatttcctta atatttttcc tgtccgtttt aaacttcgcc
     1501 ttaaacgcat ccggtaaatc tttatcgaaa taccaaagcc cgtcacccat ttccaatgcg
     1561 ccgcccattc catgcagaac aactttttcc cccgttaaag gatcagtttc ggtaatttct
     1621 acctcatgca tttttcccca atccaaaata ggcaacttgt gtgcaaacgc caaaacctgt
     1681 tcgtcagaac ggccgcacgc cttatcgcat cggcctgaaa catatacggg acaaggctca
     1741 tcctctgcat aaccgtccgg aaggataccg gctgcggcag gacacaatcc gtatgtttcc
     1801 gcccacaacg acaaagcccg tctcgctgcc tttttattga caaataatcc ggtaggcgga
     1861 ttgtccgcca caccgttttt caaagccgct gttttcgcat ccaacatgcc gtctgaacct
     1921 ttttcaaacc tgacggtcgt aaatgtttta agcagatttt tggcagatac gtaacaatcc
     1981 gccccataat gcctgaccaa ttccgcttta atcttatatg catgcaggct gcccaatgcg
     2041 ggaaaaaaac ggactgcctc cgtattgcac caatcaaacg aggcttttcc ggaatccaat
     2101 aaagccgaaa cctcgctata tacccgttca aacgtaccga tataatttat tgctcccccg
     2161 ccgtcgaaac aggcaagcac ccccataccg tcaggcaaac cgtacaactg ttccctcaac
     2221 cgttcgggca gcgcggcagg cagcggtttc ggattcatca aacggaaaca ctgcctgatc
     2281 cactcctcga ccccgtgttc cgacagactg tattccaaat aatcacacaa tgccgataca
     2341 tccgccatcg cacgatgcct gtcttccaca acaatcccca acctttcgat gatactgtcc
     2401 aggctgtgct tgtaaaattg cggatacaga caccgggaca gctgcacact gcacaaagca
     2461 ggcgatgaaa atccgatacc cgcacgatga aactcatgct ttaaaaacgt atagtcgaaa
     2521 cggctgttat gtgcaatcaa cacacaaccc ttcaataccg aaaacaactc gccggcaatc
     2581 tctgcaaaaa caggtgcgtc ggcaaccatg ccgtctgaaa tccccgtcag ccccgccaca
     2641 aactgcggaa taggtttttg aggattaacc agccactcat gccttaccac ccttccctgc
     2701 tcaaacttga ccaaagccac ttcggttacc ctgtcttcat acagattgcc gcccgtcgat
     2761 tccaaatcaa ccacggcaac aggcattcca aaccgtaaaa ataccttttc cagcaagggc
     2821 cagcgggaag caacaatcat tttattctct ttaaattcaa acaacaaacc gatattttac
     2881 acttttaagg catttcatcc aacaaaacaa ttgacagaat ccgatgatta ccctaaaatt
     2941 caaatctttc ttgcagcgca cccgtagctc agttggatag agtatctggc tacgaaccag
     3001 agggtcgggc gttcgaatcg ctccgggtgc gccagtaaga aaatacaata tgcgcccatc
     3061 gtctagcggt taggacatcg ccctttcacg gcggtaaccg gggttcgatt ccccgtgggc
     3121 gtgccaaatt ccaaatcccc gagattatcc ctcggggatt ttttattgtc tcggcaactc
     3181 gttaccatat ctttacctac ccccttcatc agaatctcag acacaatcga atcatattca
     3241 aacctttgcc gtgcaaaccg atatcccata accggatgcg gtgtccgtcc aacattttac
     3301 ccgattgaaa cgcctgatat atcgcacccc atcaaagggg cattgctttt cttaacaatc
     3361 ccctttgaca gggctttttt atgccatcat caaatttata gtggattaac tttaaaccag
     3421 tacggcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg gagcaccaag
     3481 tgaatcggct ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
     3541 taattcacta taatattttc tctcccgatt gaaacaggcg taacagaata tgcccgaagc
     3601 tccggctgct ttcttgttta ccgccgcgat atttagagta taataccaaa tttgagcaat
     3661 agttctaaaa cagttagaac catttttcat gagcctgact gattcgtaca ctcggagaaa
     3721 ctgatgcaga atatttttga ccctttggtt attcgtggaa aatcccttac ccccatcgtg
     3781 caaggcggta tgggggtcgg tgtttccgca tcgggtttat ccagcgcggt ggcacgtgaa
     3841 aacggtatcg gaacgattgc cagtgtggat ttgcgccacc ttcacgaaga cctactcgcc
     3901 gaatcccaaa tcaatccgag tgaagagaaa tatacgtctt tgaactgtac cgcattggac
     3961 agggaaatcc aaaaagccaa aagtgcttca gagggaaaag ggctgattgc ggtcaacgtg
     4021 atgaaggcgg tcaaagacca cgccgcatat gtccgccagg cttgcgaatc aggggcggat
     4081 gcggttgtaa tgggtgccgg tctgccttta gacctgccgg aaatgaccga gggctatcat
     4141 aaatatgtcg cgctgctgcc gattctgtcc gaatcgcgcg gtattaatat cgttttgaaa
     4201 cgttggatga aaaaaggcat attgcccgat gcgattgtag tcgaacatcc tgcccacgcg
     4261 gccggacatt tgggtgcatc aaccgttgaa ggcgtaaacg atgccaagtt cgacttcaaa
     4321 cgcgtgattg aggaaacgtt tgaagttttc aaaagtttag ggctggaaag cgaaaaaatc
     4381 ccgcttattc ttgcgggagg catggcaaat tttgaaaaag tcaaaaccgc cctaaagaac
     4441 tggggagcat ccgccgttca aatcggtacg gcttttgccg ttaccgaaga aggagatgca
     4501 caccttaact tcaaaaaaac gctcgccggt gcggaaactg aaaaagtagt cgaatttatg
     4561 tctgttgccg gtttgccggc gcgcggtgtc cgcaccaaat tcctagacag ctacatcaag
     4621 cgtgaaagca aacttcagac aaacgccaaa gccgacccgc gccgctgtac ccaaggttta
     4681 aactgcctaa ccagttgcgg tctgcgcgac gggctttcca aagccggaca attctgcatt
     4741 gatatccagc ttgccgccgc attccgtgga gaagtagata aaggcctgtt cttcagaggt
     4801 aaagacccgc tgcccttcgg caatgccatc cgcaccgtcc gcgagacgat acaatatctg
     4861 ctgacgggga gcgaacctgt tgcaacagta ggacgctgat gttaaaacag ccgataccgt
     4921 agcaaaatgc cgtctgaaaa ttttcagacg gcatctcttt atttgctttc agtattattg
     4981 aaaagtttgt ccatcggggt caaatcgacc gcattgcctt ggctggtaat ccatttgttc
     5041 tcgacgcgcc acacgcctgc cgcgtcctcc acgcgcacat accagtggtt ggtcggcgaa
     5101 agggttttga acaccgcctc atattccgcc ctgccgttct gcgcgctgcc gacgggcttg
     5161 agggcgacgg tttgatcgtc cgccttgcgg gtcgggtgca tcagcagcag gttcaaaggc
     5221 tgtttgccgt caaactcgcc gccgacaaac acttttgccg cattcatatc aggggaaatg
     5281 agaacctgca ccccgatatg ccgtctgacg gcttcttcat cccgatgaag ctggatgtcg
     5341 atatgcttgc cgtctttata ataatcgtcc gtaaccaaat ctgtcgcgtg ctgctgcgcg
     5401 acaaaaaaca tagcgacgct ggcgatgacg acaaaaatcg gccccgccat caagatccac
     5461 ggccagacgt gtttgtacca aggtttgatt ggagtgtttt gagacacggt tattctccga
     5521 taaaggttgc atcttcttcc aagacgaccg gcttgccgtc gggcgcgccg ctttcgcggt
     5581 attggaaggt aaattcgata gggtggctgc ctttgtccgc gtattccgga atggtggaca
     5641 cttggacggg aagggttacc gtttcgcgcg gggcaacctt gataccgcct tcgggcagcc
     5701 cggtcagggc gatttcgtca aagcctttga cacttgcggt aatgagctgt tctttttcac
     5761 ttttgttgat gatacgcagg ctgtatgcgt tttccagcca gcctttggcg ttttcgcgca
     5821 ccagtacgcc acggtctttc aaaatatcga cctcgaccat tttgcgcgtg gacaaaccgg
     5881 ccaggaaggc aatgataact aacgccaaca ccgcgccgta acctgccacg cgcggtctga
     5941 gcagccgttt tttaatgtct ttttcagaat attcgtgttc cagcgcgctt tcggtcgtat
     6001 aacggattaa tccgcgcgga tagcccattt tgtccataat ctcatcgcac gcgtcgatac
     6061 aggcggcgca gccgatacat tggtattgca gaccgttgcg gatgtcgatg ccgacggggc
     6121 agacttggac gcacatcgca cagttgatgc agtcgcccaa acccgcctct tccttattga
     6181 ccgttttctt gcgcgcgccg cgcggttcgc cgcgttccgc gtcataagaa acaatcagcg
     6241 tgtccttgtc gaacatcgcg ctttggaaac gtgcatacgg acacatatgc aggcatactt
     6301 tttcgcgcat aatgtgggcg aagaagaagg tcataaagcc gtaaaacgct gtggcaaaca
     6361 tcgcgccgcc gcctgctgct ccagtgaata aatcgggaac gaactggcgg atagggacaa
     6421 accagcctgc aaacgtgatg cccgtccacg cgcagacaag gaaaatcagc aggtatttgg
     6481 tggctttgat gcggatttta gtgaaattcc acggcgattt ttccagtttc agccgtttgt
     6541 ttctatcgcc ttcgaccagg ttgtcgatcc acagcataat ttcggtgtaa accgtttgcg
     6601 ggcaggaata gccgcaccac agtcgcccgg caatcgtcgt ccaccaaaac agcccgaagg
     6661 cgcaaatcat cagcagcaag gcaaggtaaa tcaaatcgcc cacccccaac gacaatccga
     6721 aaatgaagaa atgccgttcg gggatattga aaacgacagc ctgcctgccg ctccagttga
     6781 accacggaat gacgtaaaac acaaactgcg tcgccaatac ggcggcgata cgcagtttgg
     6841 cgaaccgtcc ttccgccttt ttgggatgga tgcgttcgcc ttcgggatgg atttgaatca
     6901 cgctggctcg cggatcgaat gttttttttg ctttcggcgc ggcttttgtt tgttcggacg
     6961 tgccgattcc ggatgccgga ctgccggctt ggttttccgt ggtcattctg cattccttag
     7021 atttttgatt gatggtttgc ccgttaccgc cgccgtttgc ttttcagacg tcatttttct
     7081 tgttttttaa ggcgttgtgt ttcaagtttt gagaaaatcc gtttttccca aaatatattt
     7141 ccgctattgt acaactttat gcgccgtctg gatgtatggg gcggatacat ttcccatccg
     7201 catcaaaacg cctggatttt accttaccgc ccgaacaaaa tccgaatacg gttaaaaaaa
     7261 aagactaaaa aaaccgacac ccccatatcg gcagaaccga cggcgcaagc tcataaacaa
     7321 acgctatcga caatccggca cacaatctat aacattttat ttcaaaagga ataatggcag
     7381 gcttcgcccg caaatcgaaa atccttcccc accggtcccc tgccgccgcc ttcccccgcg
     7441 tccgcccttt tcttgaaagc ataagcgaat cgggcgataa tcaacgccgt ctgaccatcc
     7501 acttatccga aacaccagca aggaaaatac aaaatgtctc aactggcaaa cgcaatccgc
     7561 ttcctctccg cagatgccgt tcaaaaagca aattccggcc accccggcgc gcctatgggt
     7621 atggcggaaa tggcggaaac attgtggacg aaattcctca atcacaatcc cgccaacccc
     7681 aaattctaca accgcgaccg cttcgtcctc tccaacggcc acgcgtctat gctgttgtac
     7741 agcctgctgc acctgaccgg ctacaatctg agcattgaag acttgaaaaa cttccgccaa
     7801 ctgcacagca aaacccccgg ccatcccgaa tacggctata ccgacggcgt ggaaaccacg
     7861 accggcccgt tggggcaagg gattgccaac gcggtgggca tggcattagc cgaaaaaatc
     7921 cttgccgccg aatttaataa agatggtttg aacatcgtcg atcattacac ctacgtcttc
     7981 atgggcgacg gctgtctgat ggaaggcgta tcgcacgaag cctgttcgct cgccggcact
     8041 ttaggcttgg gcaaactgat tgttttgtat gacgataaca atatttccat tgatggtaaa
     8101 gtggacggct ggtttaccga aaacatcccg caacgctttg aaagctacgg ctggcacgtc
     8161 gttcctaacg taaacggtca tgacaccgcc gccattcaag ccgccatcga agccgcacgt
     8221 gccgaaaccg gcaaaccgtc catcatctgc tgcaaaacct taatcggcaa aggcagtgcc
     8281 aacaaagaag gcagccacaa aacccacggc gcacctttgg gcgcggacga aatcgaagcc
     8341 acgcgcaaac atttgggctg gacttacccc gcctttgaaa tcccgcaaga aatttacgat
     8401 gcgtggaatg ccaaagaaca aggcgcgaaa ctggaagccg aatggaacga actcttcgcg
     8461 caatatcaag ccaaatatcc taccgaagcc gcagaattcg tgcgccgtat ggataaaaaa
     8521 ctgccggaaa actttgacga gtacgttcag acggcattga aagaagtgtg cgccaaagcc
     8581 gaaaccatcg ccacccgcaa agccagccaa aacagtatcg aaattctcgc caaagcactg
     8641 cccgaactgg taggcggttc tgccgacctg accccgtcca atctgaccga ctggtcaaac
     8701 agtgtctccg ttacccgcga caaaggcggc aactacatcc actacggcgt gcgcgagttc
     8761 ggcatgggtg cgattatgaa cggtttggta ttgcacggcg gcgtaaaacc cttcggcgcg
     8821 actttcctga tgttcagcga atacgagcgc aatgccctgc gtatggctgc gttgatgaaa
     8881 atcaaccctg tatttgtgtt tacccacgac tccatcggtt tgggcgaaga cggcccgacc
     8941 catcaaccga ttgagcaaac cgccaccctg cgcctgattc cgaatatgga cgtatggcgg
     9001 ccgtgcgaca ccgccgaatc tctggtcgct tgggcagaag ccatcaaagc cgccgatcat
     9061 ccgtcctgcc tgattttcag ccgtcaaaac ctgaaattcc aagcgcgcaa cgaacaacaa
     9121 ctgaacgata tcaaacgcgg cggctacgtc atcagcgaag cccaaggcaa cgcccaagcc
     9181 gtcatcattg ccaccggttc cgaagtcgag ctggctttgg aagcgcaaaa agtattggca
     9241 gaacaaggca tcgccgtgcg tgtcgtttcc atgccgtcca ccaacgtatt cgaccgccaa
     9301 gacgctgcct atcaaaccgc cgtcctgccc gaaggtctgc cgcgcatctc cgtagaagcc
     9361 ggacacgccg acggctggta caaatacgtc ggactgaacg gcgcagtcgt cggcatcaac
     9421 cgcttcggcg aatccgcccc tgccgattta ctcttcaaag cattcggctt taccgtagac
     9481 aatgtggttg atacggtgaa atcagtgctg taaacccaca cctaaacaaa tgccgtctga
     9541 acgcttttca aacggcattt ttttgccctc ccgccttgat gcttcctcat tccgccaatc
     9601 tccattataa tatttgcgaa tcactcttat tcacatttca aaaggagaaa cgtatgaaca
     9661 cccgcaccga acacgacacg atgggcaatg tcgaagtccc gtccgaagcc tattggggcg
     9721 cgcagaccca gcgcagccgc aacaatttca aaatcggcgg cgaaaccctg ccgcagccgc
     9781 tgatttatgc tttggcgctg gtgaaaaaag ccgctgccgc gaccaatgtt tccctcggca
     9841 ggattaagcc ggaacaggcg gatttgatta cgcaggcggc ggatgatgtg ttgaacggca
     9901 ggcttgacgg acagtttcct ttagtggttt ggcagaccgg ctccggcacg cagtccaata
     9961 tgaacatgaa cgaagtgctg gcgaaccgcg ccaacgaaat tgcaggtacg ggtttggcgg
    10021 cttatcagcc cgtccatccc aacgaccatg tgaaccacgc gcaatcgacc aacgacgcat
    10081 tcccgaccgc tatccacgtt gccgccgcga ttgaaatcaa ccgccacctc atccccgccg
    10141 taaaagccct gcgcgacacg ttggacaaaa aagcccaagc tttcgcccct atcgtcaaaa
    10201 tcggccgcac ccacttgcaa gacgcgacgc cgctgacttt gggacaggaa ttttccggct
    10261 acgtttccca gcttgatcac ggtttaggcc gtctgaacga tgcgcttaaa gacttgtatg
    10321 aacttgcttt gggcggtacg gcggtcggca cgggtttgaa cagccatccc gaatacgccg
    10381 aaaaagccgc cgccaaactc gccgaactgt ccggcttgcc gtttgtcagc gcgccgaaca
    10441 aatttgaagc cttaggcgga cgcgatgccg ccgttgccgc ttcgggcgca ttgaaaacac
    10501 tggcggcaag cctgaacaaa atcgccaacg acatccgctg gctggcaagc ggcccgcgtt
    10561 gcggtttggg cgaaatcaaa atccccgaaa acgagccggg ttcgtccatc atgccgggca
    10621 aagtcaaccc gacccaatgc gaagcaatga cgatggtgtg ctgccaagtg ttcggcaacg
    10681 acgttaccat cggtatggcg ggcgcgtcgg gcaatttcga gctgaacgtc tatatgcccg
    10741 tcatcgccta caacctcttg caatccatcc gcctgttggg cgacgcgtgc aacagcttca
    10801 acgaacactg cgccgtcggc attgaacccg taccggaaaa aatcgactat ttcctgcacc
    10861 attccctgat gctcgttacc gcgttaaacc gcaaaatcgg ttacgaaaac gccgccaaag
    10921 tcgccaaaac cgcctacaaa aacaacaaat cgttgcgcga aaccgccgtt gagttgggct
    10981 tgctgacggg cgaagaattt gacgaactgg tcgttcctgc cgatatggtt catccgcgct
    11041 aatctttccc tcaaataaaa tgccgtctga aacctcgttc ggacggcatt ttccgttgcc
    11101 tgcgaactag cggcgtttga acagcctgtc ccccaccgcc gccgtaaccg cacccccgac
    11161 cacgatcagt gcgccaacat aacccaaacc gttcatatcc ggcgcggcaa aagtatcagg
    11221 catcacataa tgcccgagca aagaaaatat tacggtaaac acggggagca aggttgttac
    11281 cgcgctgact ttggaagcct cccaatgttt caacgcctcg ccgaacgagc cgtaaccgat
    11341 taacgtattc aagcagcaat acgcaaaaca aacccacgcc aacgtaccgt ccaaacttcc
    11401 gatgtgtgcc ggttcggcaa acggcaggaa cacggcggca cttgccgcat aaatcaacag
    11461 cagaatctgt tgcggcccga attgcgccga cagcagcttt tgcgccacgg cataacacac
    11521 ccatgccata ctgcctgccg cacacagcaa cacgcccttc gcatacgcgc ccaaacccga
    11581 caactcgccg aatttatcgt taaaaaacat aagcaaaccg gcaagcagca aaaccaagcc
    11641 gattttctga gcggcagtca tccggtcttt aaacaccaac acaccgacaa caatcatcgt
    11701 aaacggcgaa atctgccaca aaacctgcgt cgtggtcggc gaaatataat gcagcccttg
    11761 ggcaatcagc acaaagtttg ccgaaatgcc cgccacgccg agcagcagca gcctgaatga
    11821 gcaccaagaa aaatcccgcc acttcggcag ccgcccgccc aatgccagca aaacaaacaa
    11881 taccgccgcc gccacggtaa aacgcaccca caccagcgtc ggcgcatcga caaacttcaa
    11941 tacctgccgc acggcaatcg gcagcgttcc ccacgtcatc gccgccaaaa gtgccaacgc
    12001 gaagcctagg agcggccttt ggttttccat cctgattttc ctatttttaa acaaccgtat
    12061 tgccggacga tgccggtttg ccgcatcggg caatgatggt tcaagcgttt gacgtttgat
    12121 tccaaccctt tgatttcaaa caaaccggct gaagcccgac tattgcttcg cgctatttgg
    12181 aaacaccgcc tgaattttaa aatgccgtct gaaaacagcg gggacgtgcg gcaggcatca
    12241 ctgcctcaaa acgcccgaac ggcgcaatcg caacgttccg cccaacgcaa ggggcggcaa
    12301 caagccggct cggatgcaaa aaagagaaac cctgccccgt aaggtttaag gtttctccgt
    12361 cctttatgat ttccctctgc gaggatgtcc ggccgtaaaa ttcagaacgg gatgtcgtcg
    12421 tcaatatcct cgaccggggc ggcggcaggc acgggttggc ggcgcggcgc ggctggtgct
    12481 tcttggggat gggacggcgc gtcggaggcg ggctgccggc tttgctgtgc ggggcgttgg
    12541 taagcctcct gactctgacc gtaaccttcc tcgtaaggcg caccgccgct gttttcattg
    12601 cgcccgccca acattttcat ttcgttggcg acaatatcgt aagcggtgcg ttcgatgccg
    12661 tctttgcctt ggtatttgcg gctttggatt ctgccttcca gataaaccag cccgcctttt
    12721 ttgaggtatt gcccggcaat ttccgccagt ttgcggtaca tcgtgatgtt gtgccactca
    12781 gtacgctcta cacgttggcc gttgcggtcg ttccaagttt cgctggtggc gacgctgaaa
    12841 ttacacaccg cctcgccgtt gggcatatag cgcacttcgg gatcgcgtcc gaggcggccg
    12901 ataaggataa ctttgttcaa tgacattttt aaactcctgt aatgattttt tcagcggcag
    12961 cctgatcgaa acccttctgc aacactttga gatagacggt ctgcccgtcg aaactgaaac
    13021 cgatgtcttc cacgccttcg agttgcaaca gggcgcgttg taagccgtcc tgattgacct
    13081 gccacacgcc gccgacaggg taactgaggt ttttgacggg cttgggcgca ggcgataaaa
    13141 cggcaatcac cagccacagc agcatcaata tgctgcaaaa ggcaaacacg ccggaaaagc
    13201 cgtatttttg aaacagcaaa ccgcctgcca cgccgccggc aaacagtccg agcgactgca
    13261 tcgtgttgta cacgcccatc gccgtaccct tcaggtcgga cggcgcgatt ttggaaacca
    13321 tagacggcag gctcgcttcc aacacattaa aaccgataaa gtaaacaacc aaataagcgg
    13381 taatcaagcc taccgagcgc ataccggaca gcaaaccgag ctgcgccgcc gcgatgcaga
    13441 cgatgcccaa aacaaaaacc tgtttgagct tgttgcgcgt ctctccgacg ataatcagtg
    13501 gaaccatcac caccaagccc gtaatggtcg agggcagata gactttccaa tgctgtattt
    13561 tttccaaacc gagctgggtc atcgcgaaag gcagcgcggt aaacaatgcc atttgcgcgg
    13621 cgtgcagggc gaaaatgccg aaatcgagcg tcagcagcct acggtttttc aaaacttcgc
    13681 ctatgcgcga aggctgcgcc tgcgtatctt cgtgcagctt ggaaacctcc gggtcgggag
    13741 tcatccacgc caccacgccg atactggcaa cggtcaaaat accggtcagc agaaacaagc
    13801 cgcgtacgcc caccgcgtcg gcaatcacag gggcaacgac gaggctgacc gaaaacgtca
    13861 gaccgatact caaaccgatc atcgccatcg cgcgggtacg tacgccgtcg cgcgtcaaat
    13921 ccgccagcag cgcggtaacc gccgcactga ccgctcccgc accctgtatg gcgcgtgcgg
    13981 cgaccagcat gggcagcgta tcggcggcgg cggcaaggaa gctgcccgct gcaaacacaa
    14041 tcagcccgac ataaatggtt ttcttgcgcc cgaacttgtc ggaagcgatg cccaaaggca
    14101 gttgcagcag agcctgtgtc agcccgtaaa tgcccattgc cagcccgacc agcgttttgt
    14161 tgccttccgc gccgggcagc gaggcggcat acaccgccaa tacgggcagc acgaggaaca
    14221 tacccagcat acgcagcgcg tacacgccgg aaagcgtcgt actggcgcgc cattcgtgcg
    14281 gaaacatttg gatgcggtta tctcttgcca tcatattttt tcagacggca tcaacagttg
    14341 caatgccgtc tgaacttcca gtgaacagat tttcggatta tacaggattc gccgtatttc
    14401 ggttgcggcg cgggttcaaa atcaacgcca ctgccagcgg ttgcgccacg cgcccaaaac
    14461 ggcgttcgga tatttattgc tgcccaagct gccgttaaag cgggcaagcg cgcggacgat
    14521 gttgcctttt tcaagattcc ggtaatggcg caggatggta cagccgtaac gcaggttggt
    14581 gcggatgtcg aacaggttgt gcgccggttt gccgatgtag tttttccaaa acggcataac
    14641 ctgcatcagg ccgcgcgcgc cgacaccgct gattgcatac tggcggaacg cgctttccac
    14701 ctcaatcagc cccaacacaa tctgcgtatc caaaccggcc cggctgcttt cgtactggat
    14761 attgaccagc agcctgcgcc gctcctcctc ctcggggacg aaccttgcca aacgtgccga
    14821 catggcagac aaccaacgtt cgccctcttt cggattgtca aacaccagcc tcggcggatt
    14881 gacgctgccg acagaactcc tcatcacgga agccacatcg tcggcaagcg tttcctcacg
    14941 ttgcgcgccg gcgtgcgcca gaggactgag caacaacgca ccggcggcac acaacaggcg
    15001 gcggcgttgc agattaacgg gtagggtatc ggtcggtttt ttcataggga acgggggcgc
    15061 gtccggacgt ttcagacggc attaaatatt caaacagaca atgattgctt ccaacgcgaa
    15121 aaaccgcgca caaaatccaa gcgcggcata tcgccctgcc cttttcgggc aaaccttgat
    15181 tttaccgccc tcaagaacgc ttgtccaaac aggcacaggc aacaccaccc gggcattttc
    15241 cgttttcacc ggttatccgt cgtccggatt atgcagcagc accatcagcg catcacgctt
    15301 ttcgggcggc agcag
//
LOCUS       DAWFBO010000032        15279 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFBO010000032 DAWFBO010000000
VERSION     DAWFBO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.032"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(107..499)
                     /gene="rpsI"
                     /locus_tag="WGO87_000735"
     CDS             complement(107..499)
                     /gene="rpsI"
                     /locus_tag="WGO87_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0392120.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(509..940)
                     /gene="rplM"
                     /locus_tag="WGO87_000736"
     CDS             complement(509..940)
                     /gene="rplM"
                     /locus_tag="WGO87_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0392121.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1118..1297)
                     /gene="ssrS"
                     /locus_tag="WGO87_000737"
     ncRNA           complement(1118..1297)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGO87_000737"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1303..1608)
                     /locus_tag="WGO87_000738"
     CDS             complement(1303..1608)
                     /locus_tag="WGO87_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0392122.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(1619..1948)
                     /locus_tag="WGO87_000739"
     CDS             complement(1619..1948)
                     /locus_tag="WGO87_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392123.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(2003..2992)
                     /locus_tag="WGO87_000740"
     CDS             complement(2003..2992)
                     /locus_tag="WGO87_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0392124.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3115..5817)
                     /gene="ppc"
                     /locus_tag="WGO87_000741"
     CDS             complement(3115..5817)
                     /gene="ppc"
                     /locus_tag="WGO87_000741"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0392125.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVTELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            5860..6753
                     /locus_tag="WGO87_000742"
     CDS             5860..6753
                     /locus_tag="WGO87_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0392126.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFDEGDVGKLKTEALADRLHHINHTVDVRTVNEKLDSGRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWRYFDLPRNPECPVCGAAR"
     gene            complement(7314..7538)
                     /locus_tag="WGO87_000743"
     CDS             complement(7314..7538)
                     /locus_tag="WGO87_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0392127.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(7539..8927)
                     /locus_tag="WGO87_000744"
     CDS             complement(7539..8927)
                     /locus_tag="WGO87_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0392128.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(9013..9834)
                     /gene="prmC"
                     /locus_tag="WGO87_000745"
     CDS             complement(9013..9834)
                     /gene="prmC"
                     /locus_tag="WGO87_000745"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0392129.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(9824..10285)
                     /locus_tag="WGO87_000746"
     CDS             complement(9824..10285)
                     /locus_tag="WGO87_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392130.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            complement(10350..11834)
                     /gene="tldD"
                     /locus_tag="WGO87_000747"
     CDS             complement(10350..11834)
                     /gene="tldD"
                     /locus_tag="WGO87_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0392131.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILTGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      11909..12012
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12078..13301
                     /gene="cytX"
                     /locus_tag="WGO87_000748"
     CDS             12078..13301
                     /gene="cytX"
                     /locus_tag="WGO87_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0392132.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            13298..14398
                     /locus_tag="WGO87_000749"
     CDS             13298..14398
                     /locus_tag="WGO87_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0392133.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNQAPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHIFVVGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            14419..15036
                     /gene="thiE"
                     /locus_tag="WGO87_000750"
     CDS             14419..15036
                     /gene="thiE"
                     /locus_tag="WGO87_000750"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0392134.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIALCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLREYVKQARGTPVVA
                     IGGIDLNNAQAVLATGVSSLAVVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         3189 a         4313 c         4262 g         3515 t
ORIGIN      
        1 tgtttttgat ttttgcccaa tctgtaatct ttagattgcc aatgggaaac cgtctactac
       61 aaataaaaaa accctgcgat aagcagggtt ttttgaattt ccaacattaa cgtttggaga
      121 attgttttgc acggcgtgct ttgcgcagac ccggtttttt acgttcgact tcgcgggcat
      181 cgcgggtaac aaaaccggct tgagacaagg cgggtttcaa cgcggcatcg aagtcgatca
      241 gggcacgggt aatgccgtgg cggattgcgc cggactggcc ggtttcgccg ccgccaacaa
      301 cattgacttt gatgtcgaaa gattcggcgt tttcagtcag aaccaagggt tggcgaacaa
      361 ccattcggct ggtttcgcgt gcgaagaatt cgtcaacggg acggccgttt acgatgattt
      421 gacctgtacc tttaatcagg aatacacgag ccactgaact tttgcggcgg cctgtgccgt
      481 agtagtattt accgttcatg tcgcgtcctt atttcagttc caaaactttg ggttgttgcg
      541 cagcatgggc gtgttccgca cccgcataca ctttcagttt tttaatcatg gcgtaaccca
      601 aaggaccttt gggcagcata ccttttacag cttgttccaa agcgcggccc gggaattgct
      661 cttgcatttc gcggaaagtg cgttcgtaga taccgcctgg gaaaccggaa tggcggaagt
      721 attttttatc ttcgaatttg gcaccggtta cacgcagttt gtccgcattg ataacaatga
      781 tgtaatcgcc ggtatcgacg tggggggtgt attcaggttt gtgtttgcca cgcagacggc
      841 tggcgacttc ggccgcaacg cgacccaaga ctttgtcttg ggcatcgatg acgaaccatt
      901 cgcgcttcac ctcgtggggt ttcgctgaaa aggttttcat agtggaaatc caaatagata
      961 tagaaagttg taaattttaa agacaggatt cgattttgtc aatcgcatta ccgcgttacg
     1021 gaaggatttt ccggatttcg gcagactgca tactgctttt tcgggcgggg cggcggccaa
     1081 tgtgaaaaac cgcatcgttg cgatgcggtt ttgaatggga atccccgcga gagccgtttc
     1141 ggccgaatcc gcttgaacct tgctgacaag gcggctgcct cgggtagttt cgggtgcgtc
     1201 cgcaaaagga cgctcgcgcc cactactgct cccggcaacc ttaagcgaac ttattggttc
     1261 aaaggaatat atgccttcgc ggacaccgca gggaaaaagg ggttattcct gcgccaagcg
     1321 ggatagtgct ttttggcagg cgttgtccat atcggctatt ttacgcgcaa aatcgccgat
     1381 tgccaaatcg ccgccgttca gggaggtttt caacaggtcg tggacgacgt tgagcgcggc
     1441 cataatgacg attttttcgc tgtccgcgac gcgtccgcct tcgcggatgg cttcggcttt
     1501 gccgttgagc attccgactg cctgcaacag tgtgtctttt tcttctgccg gcgtgttgac
     1561 ggtcagccgg gcgtgcatga cttcgatgtg gacttgttcg atgttcatcc tttaatcctt
     1621 attgctgcgt ttcctgccat tgggggaggc gcgctgccag tgcgctgatt ttttccctgc
     1681 tctgttcgag caggctgcgg tatcgtgtat tttcttctgt caggctgtcg attttgtttt
     1741 gcaggtcttc tttgagtttg ccgacttgga cgagcagggc ttcgctgagt tcgtcgacgg
     1801 cggtttcgtg ttcgagtttt tgccgctcgt gcgcccgttt gagttcggcg acggtttctt
     1861 tgaggcggcg gttttcgctg acgagggttt cgaatttttg taccagcgta taaacgctgc
     1921 tttcgagttt ttcgatattt tgtttcataa ccttacctgt ccgtatgccg tctgaaggcc
     1981 tcagacggca tctgtctgtt gtttattcaa aacgcgcgct gcgttccatc agtctttcga
     2041 caacctgttg cggggtcatt tctttgcgga tgagttgcag cagggtttgg gtaatcggca
     2101 tgtcgatttg gtacttacag gcagtattga agacttcttc tatcgtgctg accccttcgg
     2161 aaacgtgtcc gatttcgacc agcacctgat gcagttcctt gccttctgcc aaacccaagc
     2221 cgacgcggcg gttgcgtgag agtgcgccgg tgcaggtcaa gatgaggtcg ccgatgcctg
     2281 ccagccccat catggttttg ggctgtgcgc ccattgcgga ggcaaggcgg gtgatttcag
     2341 ctaatccgcg cgtaaccagt gcggcacggg cgttaagccc gtactctagg ccatcggaca
     2401 atccggtggc aatcgccata acatttttta ccgcgccgcc aaccgccacg ccgataacat
     2461 cggtactgcc gtaaagcctc atgacggtcg tgttgagctg cggtacgagt tcttcaatcc
     2521 actcttggtt ttcggaggcg aggacgacgg cgcagggcag ttgtttggcg agttcctgtg
     2581 caaaactcgg accggaaagt acgccgattt ttttattgtc gggcaatact tctttcaata
     2641 cttgaaaggt cagcagccct gtatcctgct cgaatccttt gcaggcggcg aggacgggga
     2701 ggtgtcccgc gccgtactgt ttgagcagct ctgcgctgct tctcaatccg gcaacggagg
     2761 ttacgataag gacaagtccg ctgtctttga gcgcgtctgc caaatccgca cacacttcca
     2821 aggtttcggg aaaggaaaag ccgggcagtc cgcgtttgtt ttcacgcgct tcctgcattt
     2881 ggcggacttg gtctgcgttg cgcgtccaca gggatacgcg gttgccgtgt tgggaaaagt
     2941 gcagggcgag cgccgtaccc cacgaacctg cgccgataac ggtaattttc attggtcgtc
     3001 tttcaacata tcactgccgt tcactttaaa acaatcggtg tttctctgca agtgcggtca
     3061 gggaaatgcc gtctgaaagg tgttcagacg gcattttgcc ccgatgcggc acaatcagcc
     3121 tgtattgcgc aaaccttgcg ccacgccgtt gatggtcagg tgaaccatca gcagcgcgtg
     3181 cggattgtcg ggttctttac gcaggcgttt gagcatggcg acctgcaggc cgttgagcgc
     3241 gttcaaatat ggaatcctca aggcaagcga acgggcgagg ctgcggttgt cgcgtaaaag
     3301 ttcttcggtt tgcagcaggt cgagcagggc tttgcggctg cgttggtatt cttccttaat
     3361 catgccgaag atgacggcgg ctttttccgg cgattcgctc aagccggcat agttttccgc
     3421 gagggtgatg tcggttttcg ccatcacttg ctccatattg gaaagcatgg cttggaagaa
     3481 cgggttgctt tgggcgtgtt cgcgcagggc ggcgagcgtt tcgggtttgt cttcgcacaa
     3541 ggtttccacc gcgctgccga aaccgtacca agcaggcagc atgaggcggt tttgcatcca
     3601 tgagaatacc cacggaatag cgcgcaagtc ctgaatccgc gccaaggttt tgcggctggc
     3661 aggacggctg ccgaggttga gggtggcgat ttcctgaatc gggctggttt gcaggaagta
     3721 gtcgatgaag tcggggtggg taatcagttc gcggtagtat ttgaacgata cgtccgacaa
     3781 tgcctgcatc agtttggcat cagggtcttt tttatccggc aggatgctgg cttccaaagt
     3841 cgcggcaacc aaggtttcca agttgcgttg ggcattgccg gggtcggcgt atttggcggt
     3901 aatgacttcg ccttgttcgg taatgcggat ttgtcccgcc acgctgcccg ccggttgggc
     3961 gagaatggct tggtaagaag ggccgccgcc gcgtccgacg ctgccgccgc gaccgtggaa
     4021 caggcgcatg cggacatcgt attttttgaa gagttcgacc aagcccaatt ccgcctgata
     4081 cagacaccat gagctggtaa cgtagccgcc gtctttgttg gagtcggagt agccgagcat
     4141 gatttcttgg atgtttccac ggctttcgag cagggcatcg taccagtcga ggcggaacat
     4201 ggtttccata accggacagg cgttttcgag cgcttcgatg gtttcaaaca gcggcacgat
     4261 attgatgcgg ctgcgcggtt tgccgttttc caccgccaac aggccgcttt ctttcagcag
     4321 caatgccaag gcgagcaggt cgctgggttg ttcgcagttg gaaataatgc tttgtgtaac
     4381 ggcatcttcg ccgaattcgt ctttgatttt gcgcgcttcg ttgaaaattg ccagttcgtg
     4441 gcgggtatgg tcgctgtatg tgatgaacgg gctgtacagc gggcgttgat ggctcaattc
     4501 gcgcaacagg acggcttgtt tttgctcttc gttcaggctg ttgtagtctt ccaagcctgc
     4561 gtgttggaaa agctcggtaa ccacatcggc gtgtttgcct gcgtgttggc gcaggtcgag
     4621 cggcatcatg tggaagccga acacggacac ggaacggatg aggtctgcca aacggccttc
     4681 ggcaagcagg cggctgccgt tgtcgataag ggaacgttgc aattttttca aatcatcgag
     4741 aaattcttgt gccgaagcat aaggctcgag aaagccgaat ttgcagccca tgcccaaacc
     4801 gagcgcgcgc gctttgccca tagcgcgcgc cataatgtag gcaatggcgc ggcggtaggg
     4861 ttcttccgtg cgggcaagtt cttcgtcagg cgagagggct gccagtgcca ttacgtcgtc
     4921 gttgactttg acgcggcgga tggagagcgg caattcgcgg tagagtttgt cgagttcgcc
     4981 acggtaaaaa cggaacacgg catcggcgtg gcggcggaag gcaaagcgca gggtttcggc
     5041 agaaacgaac ggattgccgt cgcggtcgcc accgatccag ccgccgattt tgaggatgtt
     5101 cggaacgcgg acatcgggat aggccgtctg aaagtcgtgt tccatcttgc ggtagagttt
     5161 gggcagggct tcaaaaaagc tcatcgggaa gatggacacg ccgttgttga tttcgtcgtt
     5221 gacgctgagt ttgtggcggc gcgtttcgct ggtctgccac aaacccagca ggatagtgtc
     5281 gatttcgcgg cgcagccgtg ccagcgcgtc ggcattggtg cagcgttcgc gttgcggcag
     5341 cagcgcgcgg atgcggcggt tgaaattcaa aacggtttgg cgttgcactt cggtcgggtg
     5401 cgcggtcaaa acggcggtaa cggacgtatt gtccaactgc cgctgcaccg atttgccgtc
     5461 ggctttcccc gctttgagcc tgcggacggt ttccgtcagg ctgccttccg cgccgccgcg
     5521 tccggcttct tcgtggattt ggcggcggcg ttcgtggtgc acgtcttcgg cgatattcaa
     5581 aatctgggcg aacaggccgc aggccaaggt taaatcgtgg gtttgttgtt cgtccaattg
     5641 cggcaatact ttttcaatca atgccgcgct gtcgtcggaa gtggacaaga gtttgaccgt
     5701 ttcgacaacc aatggcgagg cttcttcgtg caggaggttg aacagggatt gtttcagaaa
     5761 ttccgcgtcc gccgccaaag ccgcgtcctt tggattgttc agaatatgca gttgcatgat
     5821 ttttctctca ttgccgtaaa tactgtaaat gtacctcaaa tgccgcatcc gtgccaaacc
     5881 gttcacactt taaccgcccg tgtcccgaaa tgccgtctga agttgaacgc cgcccgacgg
     5941 cagcgttaca atcgcccgca actgtttttt ccgaacatca tcatgaccac gaccgaacac
     6001 gacaacgacg atgcattcct gctgcggtac agccggcaca tcctcttgga cgaaatcggc
     6061 atcgaagggc agcagaaact ttccgacgcg catattttgg tcgtcggatg cggcggtttg
     6121 ggtgccgccg cactgcccta ccttgccgct tcgggtgtcg gcacgctgac catagccgat
     6181 ttcgacacgg tcgaactgca caacctgcaa cgtcaggttg catttgacga gggcgacgtt
     6241 ggcaaactca aaaccgaagc cttggcagac cgtctgcacc atatcaacca taccgtcgat
     6301 gtccgcaccg tcaacgaaaa actcgacagc ggccgcctga ccggtttggt tcaaaccgcc
     6361 gatatcgttt tagactgctg cgacaactat gccacgcgcc aagccgtcaa ccgtgcctgc
     6421 gtacaaacga aaacaccgct ggtttcagga gcagcggtgc gctttgaagg gcaacttgcc
     6481 gtgtaccgac ccgacttgcc cgactcgcca tgttacgcct gcctgttcga cggcggatcg
     6541 gcttcagacg gcatctgttc ccttttcggc gtattctcac cgctggtcgg catcatcggc
     6601 tgcacgcagg cagcggaggc actgaaaatc ctgctggatg caggcgaacc gtcgcacggc
     6661 aggctggcgg tttaccgtgc tttggaaggg ggctggcgat atttcgacct gccgcgcaac
     6721 cctgaatgcc cggtttgtgg cgcggcacga taaaccctgc cgccgtttca gacggcataa
     6781 aaacggattc ggcggaacgg ttttaaaaat cggcggtgtt ttgacatagt gtcgataagc
     6841 atacttatct tcaatacact caacaggata attgtttgcc taagcaaaaa aataatcgcc
     6901 ttttccctga cgggcgggcg aaatccacgg attgcaaaaa cacaacctga aaatttacat
     6961 ttctttgcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ataaacttac cttataatta
     7021 cggatgtctt agcaatatct gcttcgcaga ctcattgagt aagatatttt ccccgtaatg
     7081 tgtttggccg tctgtcccct ttgggttcgg atggcttttt tttttgactg tgtttgaata
     7141 cccggttgat ttcgtctgtt tgcaggggga aagccgctta tttccgttcg ggtggaaaac
     7201 ggttccatcg gataaaaggc attttgtccg actgattaaa gttacatctt agagccgcat
     7261 cggcggaata ttcaaacaca gcttttttaa ggaaatccgg atacggcggc gcatcaataa
     7321 tgcggcggaa tctcgtcgcg cagggaatac ggctcttgcg cgtcgggatt cctgtcctgc
     7381 attttttgat acagcagcct caactgagcc tgttgcaaat ccagcgtctg ccgcaattcc
     7441 gccaccatcg cgttcaggcc ggcgattacg tcctcctgaa gcgcggattg gatttccagc
     7501 tctgtgatgc ggtgttccaa ctcttgaacc gcgtccattt acagcaccat cgcggcaatc
     7561 cagccggcaa tcagcagcgg gatgttgtag tggatgaagg tcgggataac ggaatcgcgg
     7621 atgtggtcgt gctgcccgtc ggcgttcagc cccatcgtcg gacccagcgt ggaatcggac
     7681 gcaggcgaac cggcatcgcc caacgccccc gccgtgccga caatggcgac ggtggcaagc
     7741 ggcgaaaaac ccaaaccgac acacaaaggc acataaatcg cggcaataat cggcaaagtg
     7801 gaaaaggacg aaccgatgcc catcgttacc aaaagtccca ccaccagcat cgccaacgcc
     7861 gccatacctt tgctgttgcc gaatatcgcc atactgcttt ccaccagcgg ctggatatgc
     7921 ccggtcgcat tcatcacggc ggcaaaaccc tgcgcggcaa tcataatgaa gccgaccatc
     7981 gccatcatct tgataccttc gccgaatacg tcgtttgcct tgtcgcggtt gatgaccccc
     8041 aacatcataa atacggcgaa accgagcatc gcgcccaaca ccagcgagtc ttcatacatc
     8101 aactggatgg caaaacatac ggcaatggcg gcggcggcgg ccaggctgcg gtaggcggac
     8161 ggctgcggac ggtttgccgc atcggcgttg cccgctgtat cggcattgtt gctttggtac
     8221 aggcgcggtt tgcggtaatg gacaaacgcc agcaggagtc cggtcagcat tcccaacgcg
     8281 ggaatcgcca ttgccgccat tacgttgatg tttttcacat caagctgcgg cgcggcggaa
     8341 tggatgttgc ccaacaggat ttcgttcaaa aaaatcgcgc cgaagccgta aggcaggaac
     8401 atataggtcg taaccagccc gaaagtaatg acgcacgcaa tcagacggcg gtcgattttc
     8461 aggcggttga acaccaaaag cagcggcgga acaatcatcg ggataaaggc gatgtggata
     8521 ggtatgatgt tctgactcat aatgcccatc acaaggatga tggaaagcag cagccatttg
     8581 accgcgccct cgcccgaacg cacgctgtcg ggcataccgc cccggttcag cttgcggacg
     8641 accgcgccgg caagctgctg cggcaggccg gaatgggtaa tcgccattgc aaacgcgccg
     8701 agcatcgcat aagaaagcgc aatcttcgca ccgccttcca aacctttgtt gaacacgggg
     8761 ataatccccg cctgactgac ctgtcccgcc gcatcggcaa tgttttgcag cggcataccc
     8821 gccaccgcgc cgccgacaaa cgcgccgacc gtcaggctca ataccacgtg cacgcgcgac
     8881 agcgacagca ccagcataac gattacggca actacgactg cattcattgt ttatcgctcc
     8941 aaacctataa atgtttacat atcgaaacac atcataaccc aataatggaa aacccgccaa
     9001 ttttgcaaac aattatttca aatgcttcat atacttcccc agcgtaaccc tgtccaaacc
     9061 cgccaagtcc ggcagggttt ccactcccga aaaaccattc tccgccaaca cgccgcgcac
     9121 cgccgcgccc tgatcgaaac cgtgttccag caataaaaaa ccgccttccg ccaaacggtc
     9181 gggcgcgcct tgcgccaagg tgcggatgca gcttaggccg tctgaaaagt cggtcagcgc
     9241 gatttgcggc tcaaaccgca aatcgccttg caacaaatgt ttatcgccgt tttcgatata
     9301 gggcgggttg gacacgatga tgtcccattt cccttcagac ggcatatcgg tgtcgaacca
     9361 cgaaccgtgt gcaaattcga cccgcgcgcc caaatctgcc gcatttttcc gcgccgtttc
     9421 aagggcgggc gtgctgatgt cggatgcgcg cacaaacgca tcggggcgtt cgagtgcgac
     9481 ggttacggca accgcgccgc tgcccgtccc caaatcccac acgcgcccgt tttcgggcag
     9541 gcgcgccaat acggcttcga ccaagtgttc ggtttcgggg cgcggaatca gcacgctcgg
     9601 attgactgta aagcgtctgc cataaaattc gcgcacacct aaaatatagg caaccggctc
     9661 gccgttcaga cggcgttgcg ccagcctgtc cgcccgctgt cggacttcgt ccggcatttc
     9721 ttccccgccc cgcgtcaaca actgcacgcg cgtatattcc gaaacatatt gcagcagcat
     9781 tcttgcttca attttaggca gttttgacaa gcccaaccat tcgtcaaacg tcatttttta
     9841 tcccgtctgc cgccgatgcg gcttttcttt ccttattctt tccggcaaac gtaccgatgg
     9901 tggcaaccgc aaatgcggca taccacaaat aaaatcctgc accgtagcgc acaatatccg
     9961 atgtattccc tgcttcatcg acgtatacgg ctttcacact gaaagccacc aacgccaagc
    10021 cccaaagtgc cgcatggaca ggcacgacct tcttccgcaa cgccagcaaa acaatggccg
    10081 ccaaccaaac ataattcgca tagaccgcac aatacctgat atccaaagaa gcaaatatcg
    10141 accccaaaat caaaacggtc aaaccctcca tgcttccatg attgcccaaa taaaatgcaa
    10201 cattggataa agacgctatc cacagggcaa ccgacaccag caacatcact atgggaaaac
    10261 ttgatttccg attctgttcc tgcatggttt tatcctaatg taaaaggccg cctgaaaacc
    10321 tttcagacgg catcgtgccg gattccgcgt cagattgcgc tgccgccgac ggtcagtccg
    10381 gcatcaatcc gcaaagtcgg ttgccccacg ccgacgggga cgctctgccc ttctttgccg
    10441 cacacgccga caccgctgtc gagcgcggta tcgttgccta tcatcgaaac gtgtttcagc
    10501 acttcgggac cgttgccgat aatggtcgcg cctttgacgg gatattgcag cctgccgcct
    10561 tccacccacc atgcttcgga cgcgctgaac acaaatttgc cgctggtaat gtccacctgt
    10621 ccgccgccga agttgacggc gtaaatgcct ttgtcgatgg acgcgatgat ttcttccggc
    10681 tcatagctgc cgttttccat aaaggtattg gtcatgcgcg gcataggggc ggaggcgtag
    10741 ctttcgcggc ggccgttgcc ggtcgattgc gtacccgtca ggcgggcgtt ggtttcgtcc
    10801 tgcatatagc cggtcaaaat accgtcttca atcaatacgg tgcggcgggt ttcgttgcct
    10861 tcgtcgtcga tgttgagcga accgcgccgg ccggcaatat cgccctgatc gacgacggta
    10921 acgcctttgg cggcgacgcg ctcgcctatt ctgccggaaa agacgctggt ccccttgcgg
    10981 ttgaaatcgc cttccaaacc gtgtccgacc gcttcgtgca gcaacacgcc cggccagccg
    11041 ttgcccaaaa cgacggtcat ttcgccggcg ggcgcggggc gggattcgag gttggtgagt
    11101 gcctgtttga cggcggcatc gacaaaccga tgaaccaagt tttcatcgaa ataagccaag
    11161 tcgtagcgtc cgccgccgcc cgcgctgccc tgttcgcggc gttcgccctg tttggcgata
    11221 acggtaacgt tcaggcgcac catcgggcgg atgtcggcgg cgtgtttgcc gtccagacgg
    11281 gcgaggtaaa ccatatcgta ttcgcaggtc aaaccggcca tcacttgcac gatgcgcgga
    11341 tcggcggctt tggcgattgc ttccactttg ttcaacagcg cgactttggc ggcggaatcg
    11401 aggccggcaa tggggtcgga cgcggaacaa accggcttgc cgcgcgtttc agacggcatt
    11461 ttggcggaca ccttgccgcc tgccgcccca atcgcgcgga cggcgcgggc ggaacggttt
    11521 atcgaatcga tgcacaggct gtcggcgtag gcaaaggcgg ttttgtcgcc cgaaacggcg
    11581 cgcacgccca cgccctgatt gatttggaag ctgcccgatt tgacgatgcc ctcttccaaa
    11641 tgccagcttt cataagcggt gcgctggcag tagatgtcgg cgtaatcgac gtggtgcgcg
    11701 ccgatgatgc acaggctttt ggcgagcagt tcgggggaaa ggcggttggc ttcgagcagc
    11761 cgcgcctgta cggcggaata ggtcggatgc atagtgtcgg cgcataaaaa atcaggggct
    11821 tgattatacg gcatttgtta tggaaatgcg ccgtgccgcc tgaaatgtaa gatttttgcc
    11881 aacgccccct gcttttgtgt acacttaaag ctccttgtcg gagtgccgcc gccgggcggc
    11941 tgagattgcg aaagcagaat ccgtagaacc tgtcggggta atgcctgcgt aggaaacaaa
    12001 acctccccgc cctttcgggc ttccgttccc ttttccgcac ttccccgccc cattttcatg
    12061 ttttttaagg acttgatatg tcgggcaatg cctcctctcc ttcatcttcc tccgccatcg
    12121 ggctgatttg gttcggcgcg gcggtatcga ttgccgaaat cagcacgggt acgctgcttg
    12181 cgcctttggg ctggcaacgc ggtctggcgg ctctactttt gggtcatgcc gtcggcggcg
    12241 cgctgttttt tgcggcggcg tatatcggcg cactgaccgg acgcagctcg atggaaagcg
    12301 tgcgcctgtc gttcggcaaa cgcggttcag tgctgttttc cgtggcgaat atgctgcaac
    12361 tggccggctg gacggcggtg atgatttacg ccggcgcaac ggtcagctcc gctttgggca
    12421 aagtgttgtg ggacggcgaa tcttttgtct ggtgggcatt ggcaaacggc gcgctgattg
    12481 tgctgtggct ggttttcggc gcacgcaaaa caggcgggct gaaaaccgtt tcgatgctgc
    12541 tgatgctgtt ggcggttctg tggctgagtg ccgaagtctt ttccacggca ggcagcaccg
    12601 ccgcacaggt ttcagacggc atgagtttcg gaacggcagt cgaattgtcc gccgtcatgc
    12661 cgctttcctg gctgccgctg gccgccgact acacacgcca agcacgccgc ccgtttgcgg
    12721 caaccctgac ggcaacgctc gcctatacgc tgacgggctg ctggatgtat gccttgggtt
    12781 tggcagcggc gttgttcacc ggagaaaccg acgtggcaaa aatcctgctg ggcgcaggtt
    12841 tgggtgcggc aggcattttg gcggtcgtcc tgtcgaccgt taccaccact tttctcgatg
    12901 cctattccgc cggcgcgagt gcgaacaaca tttccgcgcg ttttgcggaa acacccgtcg
    12961 ctgtcggcgt taccctgatc ggcacggtgc ttgccgtcat gctgcccgtt accgaatatg
    13021 aaaacttcct gctgcttatc ggctcggtat ttgcgccgat ggcggcggtt ttgattgccg
    13081 actttttcgt cttgaaacgg cgtgaggaga ttgaaggctt tgactttgcc ggactggttc
    13141 tgtggctggc gggcttcatc ctctaccgct tcctgctctc gtccggctgg gaaagcagca
    13201 tcggtctgac cgcccccgta atgtctgccg ttgccattgc caccgtatcg gtacgccttt
    13261 tctttaaaaa aacccaatct ttacaaagga acccgtcatg acccgtatcg ccatcctcgg
    13321 cggcggcctc tcgggaaggc tgaccgcact gcagcttgca gaacaaggtt atcagattgc
    13381 acttttcgat aaaggctgcc gccggggcga acacgccgcc gcctatgttg ccgccgccat
    13441 gctcgcgcct gcggcggaag cggtcgaagc cacgcccgaa gtggtcaggc tgggcaggca
    13501 gagcatcccg ctttggcgcg gcatccgatg ccgtctgaac acgcacacga tgatgcagga
    13561 aaacggcagc ctgattgtgt ggcacgggca ggacaagcca ttatccagcg agttcgtccg
    13621 ccatctcaaa cgcggcggcg tagcggatga cgaaatcgtc cgttggcgcg ccgacgacat
    13681 cgccgaacgc gaaccgcaac tcggcggacg tttttcagac ggcatctacc tgccgaccga
    13741 aggccagctc gacgggcggc aaatattgtc tgcacttgcc gacgctttgg acgaactgaa
    13801 cgtcccttgc cattgggaac acgaatgtgc ccccgaagac ctgcaagccc aatacgactg
    13861 gctgatcgac tgccgcggct acggcgcaaa aaccgcttgg aaccaagccc ccgagcacac
    13921 cagcaccctg cgcggcatac gcggcgaagt ggcgcgggtt tatacgcccg aaatcacgct
    13981 caaccgcccc gtgcgtctgc tgcatccgcg ctatccgctc tacatcgctc cgaaagaaaa
    14041 ccacattttc gtcgtcggcg cgacccaaat cgaaagcgaa agccaagccc ccgccagcgt
    14101 acgttccggg ctggaactct tatccgcgct ttatgccgtc catcctgcct tcggcgaagc
    14161 cgacatcctc gaaatcgcca ccggcctgcg ccccacgctc aaccaccaca accccgaaat
    14221 ccgctacagc cgcaaacgcc gcctcatcga aatcaacggc cttttccgcc acggtttcat
    14281 gatctccccc gccgtaaccg ccgccgccgt cagattggca gtggcactgt ttgacggaaa
    14341 agacgcgccc gaacgtgatg aagaaagcgg tttggcgtat atccgaagac aagattaaag
    14401 ccgtctgaaa ggaaccccat gaccttcccg ccactcaaat ccccgctcaa attctacgcc
    14461 gtcgtcccca ccgccgattg ggtggggcgc atggtcaaag caggtgccga cacggtgcaa
    14521 ctgcgctgca aggccctgca cggcgatgaa ttgaaacgcg aaatcgccct ctgtgtcgcc
    14581 gcctgtcagg gcagccatac gcagcttttc atcaatgacc actggcgcga ggcaatcgaa
    14641 gcaggcgcgt acggcgtgca tctcggacaa gaagacatgg acaccgccga cctcgccgcc
    14701 attgccgctg ccggtttgcg cttgggtttg agtacgcact ccgtcgccga actcgaccgc
    14761 gccctgtccg tgcaccccag ctacgtcgcc agcggcgcga ttttcccgac cacgaccaaa
    14821 caaatgccca ccgccccgca aggcttggac aagctgcgcg aatacgtcaa acaggctcgc
    14881 ggcacgcccg tcgtcgccat cggcggcatc gatttgaaca acgcccaagc cgtgctggca
    14941 acaggcgtat cctcactcgc cgtcgtccgt gccgtgaccg aagcggcaaa tcccgaagcc
    15001 gtggttaaag cgtttcaggc tttgtgggat ggataaaacc gaaagaagaa aattcaattg
    15061 ccgtgtaggc aaaacttagc ccgttatcgc aaacatactt aactttaaat gtggcatatc
    15121 atcaaattcc gtcatttccg cgcaagcggg aatccgcctt aaaacttgag aaaccatcat
    15181 ttgaaaaaca gtttccgaat ttcaaaaatg gattcccgcc tgcgcgggaa tgacggcaac
    15241 cggccggttg cgtatcaaaa aataaagtaa ttcggctag
//
LOCUS       DAWFBO010000033        14917 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFBO010000033 DAWFBO010000000
VERSION     DAWFBO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.033"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGO87_000751"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGO87_000751"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0392135.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1965..3572)
                     /gene="aceF"
                     /locus_tag="WGO87_000752"
     CDS             complement(1965..3572)
                     /gene="aceF"
                     /locus_tag="WGO87_000752"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0392136.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGTAIIEVETAGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAAGASLGSSLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPIWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3722..6385)
                     /gene="aceE"
                     /locus_tag="WGO87_000753"
     CDS             complement(3722..6385)
                     /gene="aceE"
                     /locus_tag="WGO87_000753"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0392137.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7079..8791
                     /locus_tag="WGO87_000754"
     CDS             7079..8791
                     /locus_tag="WGO87_000754"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0392138.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFKGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            8854..9510
                     /locus_tag="WGO87_000755"
     CDS             8854..9510
                     /locus_tag="WGO87_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0392139.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFAAADRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9520..9975)
                     /gene="ruvX"
                     /locus_tag="WGO87_000756"
     CDS             complement(9520..9975)
                     /gene="ruvX"
                     /locus_tag="WGO87_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0392140.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9968..10516)
                     /locus_tag="WGO87_000757"
     CDS             complement(9968..10516)
                     /locus_tag="WGO87_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0392141.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10518..11078)
                     /locus_tag="WGO87_000758"
     CDS             complement(10518..11078)
                     /locus_tag="WGO87_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0392142.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11406..13211
                     /locus_tag="WGO87_000759"
     CDS             11406..13211
                     /locus_tag="WGO87_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0392143.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13338..14822
                     /locus_tag="WGO87_000760"
     CDS             13338..14822
                     /locus_tag="WGO87_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0392144.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKLIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQEKTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNGETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3536 a         3696 c         4208 g         3477 t
ORIGIN      
        1 tcggagaaga tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccta
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatca aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagca gcaacgtggc
      541 cttcgtgaac ggctttgtgt gccaacattg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggtaacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtaacgttgc ggctctttag gcgcatttgc accttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagtgg
     1141 ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc caagtggtgc gggtctaaga
     1201 attgcccgtc gccttggata acgtccactt tacggctttt cgccatacct gccaaaccgc
     1261 ccgtgaggcg ggaaactacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg accgccaccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ccgcttcggc tttaggagcg gcagccgtgc cttcagcttc aacgacaaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcgcc cacgtttact tcaaccgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aagctcatgt ctcatctttc tgaatgatgt tcaaacttcc
     1921 ttttcagacg gcacagaccg ctatgccgtc tgaagccgat cgggttacag ggtaatgcgg
     1981 cggaagtctt tcaacagctt cgccaagaat acggtaaagc gcataccggc cgcaccgtcg
     2041 atgacgcggt ggtcgaagga caggctcaac gggcacatca ggcgcggggc aaactctttg
     2101 ccgttccaaa tcggtttgat ttgggatttg cacacgccca agatggcgac ttcgggagcg
     2161 tttacaatcg gcgtgaagcc tgtgccgccg ataccgccca agctggaaat ggtaaagcac
     2221 gcgccttgca tttcttgcgg tttgagcttg ccttcgcggg cttttttgga caattcggtc
     2281 agctcttggc tgatttgttt caagcctttt tgatccacgt ctttgatgac gggaacaacc
     2341 aagccgttcg gcgtatcggc tgcgaaaccg atgttgaagt agtttttcag caccaggttg
     2401 tcgccgtcca gagaagcgtt gaattcgggg aaggctttca gcgcggaaac ggaggctttg
     2461 atgatgaacg ccaacggtga cagtttcacg ccttcgcgtt cccattcttt gttcagttgt
     2521 ttgcggaatt cttccagctc ggtcatatct gcttcttcgt gtacggtaac gtggggaatc
     2581 accacccagt tgcgagacag gttttgaccg gagattttct taatgcggga caattcttta
     2641 acttcgacat tgccgaattt ggagaagtcc actttaggcc acggcagcaa gtccagactg
     2701 ctgcccaaag atgcgccggc tgcggcaggt tttgccgcgc caccctgcat tacggatttc
     2761 acaaaggctt tgatgtcgtc gcccacgata cggcctttca agccggtacc tttgacttgg
     2821 cccaaatcca cgcccaattc gcgcgccagt ttgcgtgcgg aagggccggc gtgtgctttg
     2881 gcgaaagcgg cctcgtcgat tttggcagcg gcaggtgcag aaggtgcagg cgcggcggcg
     2941 ggtgcggcgg cagcagtcgg aggcggagcg gcagccggtg cggcagcttg agcggcttga
     3001 gcaggggctg ctgcggcaga gccggcggtt tctacttcga taatggcagt accttcggac
     3061 actttgtcgc cgacttttaa gaatacggct ttaacgacac cggcagcggt acaaggtacg
     3121 tccatcgttg ctttgtcggt ttccaaagta atcagcgtgt cgtcttcggc aacggtatcg
     3181 ccgactttga tctcgacggc gattacatcc acatcggtat ggccgccgat gtcgggaacg
     3241 gctacttgaa cggttgcacc gcctgcggca acgggtgcgg cagcaggtgc aggttgtgct
     3301 tcggcagcag caggagcggc ttcggcagcg gcggcaccgg tttcaacggt caaaattacg
     3361 ccgccttcgg agattttgtc gccgactttg actttaactt ctttcacgac accggccgca
     3421 tcggcaggca catccatcgt ggctttgtcg gtttccaaag taatcagggt gtcgtcaacg
     3481 gcgatggtgt cgcccgcttt aacttctacg gcgatgatgt cgacgttttc gtgaccgccg
     3541 atatcgggga ctttgatttc tacgatactc atttgagttc ctttaatctt tttcagttgg
     3601 atgccgtctg aaaactgctt cacgcggcat cggtacggtt tgtctgttca aaccattcgg
     3661 gcattcggtt ttcagacggc atgatgtctg aatggggcaa acaggccgtc tgaaacatca
     3721 atcagcgttt ccagctaggg gctgaatcgg ctttgatgcc gtatttttca atggcttgtt
     3781 gaacagtttc tttgctgact ttgccttgtt ccgccaatgc ggccaatgct gctacggcaa
     3841 cgttgtagcg atccacttca aagaagcggc gcaggttggc gcgactgtcg gaacggccga
     3901 aaccgtcagt gcccaagacg tggtagtcgt tagggatgta ggcacggatg cggtcagcat
     3961 agctgcggat atagtcggta gcggcgataa ccggaccgtc atgaccttgc agttgagagg
     4021 taacgaaagg cactttttca gcttccagcg gatgcaggcg gttgaagcgt tcggtttcga
     4081 tggcgtcgcg gtgcagcagg ttgaaggacg ggcaagacca gatgtctgct tccacgccga
     4141 agtcggcttt cagcagctcg gcaccggcaa tcacttcttg cagaatcgta ccggaaccca
     4201 tcagttggac tttcttgtcg cctttgccgc cggctttcag caggtacata cctttcaaga
     4261 tgtcttgttc cgcaccttcg ggcatatccg gatgggtgta gttctcgttc atcagggtga
     4321 tgtagtagaa cacgtcttca ttattggcat acatacggcg cagaccgtct tgtacgatga
     4381 cggcgacttc gtattggaaa gtcgggtcat aagatacgca gttcggaatc aggtcggcct
     4441 ggatgtggct gtggccgtct tcgtgttgca ggccttcgcc gttcagcgtc gtacggccgg
     4501 cagtaccgcc cagcaggaag ccgcgcgcgt gcatatcgcc cgccgcccaa gccaagtcgc
     4561 cgatacgttg gaaaccgaac atagaatagt aaatgtagaa cggaatcatg gcgaagttgc
     4621 tgttggcgta gctggttgca gccgcaatcc agtcggccat cgcgcccggt tcgttaatac
     4681 cttcttgcaa gatttgaccg tcaacggatt ctttatagaa catcagttgg tctttgtctt
     4741 gaggggtata ttgctgacct ttcggattcc aaataccgta ttggcggaac ataccttcca
     4801 tgccgaaagt acggctttcg tcgggaacga taggtacgac gcgtttgccg atttttttgt
     4861 ctttcagcaa agtggacagg atgcggacga atgccatcgt ggtcgagaac tcgcgttcac
     4921 cgctggattt gagttgtgcg tcgaatgctg acagctcggg cacttccaat acttcctgcg
     4981 tcggtttgcg ttgcggcagg tagccgccca aagcatcgcg gcgtgcgtgc aggtatttgt
     5041 attcttccgt atcgggggca aaagtcaggt aaggcagatc gccgctttcg atttgttcgt
     5101 cggtaaccgg aatgtcaaag cggtcgcgga attgtttcag ggacgctttg tccatttttt
     5161 tggcttggtg ggcaacgttc tgaccttcgc cggatgcgcc cataccgtaa cctttaatgg
     5221 ttttcgccaa gatgacggta ggtttgccgt cggcatggtt cgctgcgcgg tcgtaggcgt
     5281 tgtacacttt ttgcgggtcg tggccgccgc ggttcaatgc ccagagttgc tcatcggtca
     5341 tatcggcaac caatgctttc agttcgggcg tattgaagaa gtgttcgcgc acatacgcgc
     5401 cgtctttgga tttgtaagtt tggtagtcgc cgtccaaaca ttcttccata cgttggcgca
     5461 ggataccgtc tttgtctttc gccaagaggc ggtcccagcg gcggccccaa atgactttga
     5521 cgacattcca gccggcgccg gcaaagttgc cttccaattc ttggatgatt ttgccgttgc
     5581 cgcgtaccgg accgtccaag cgttgcagat tgcagttgat gacgaaaatc aggttgtcca
     5641 agccttcgcg tgcagccagt gcgattgcac cttgagattc gggttcgtcc atttcgccgt
     5701 cgccgcagaa acaccatact ttacggcctt tggtttttgc caaaccacgc gattccaagt
     5761 atttcaggaa acgcgcctga taaatcgcca tgatgggccc caagcccatg gatacggtcg
     5821 ggaactgcca aaagtcgggc aagaggtggg ggtgcggata ggaaggcaga ccgtgtccgt
     5881 ccacttcttg gcggaagttg ttcagctggt cttcggtcag acggccctcg acgaatgcgc
     5941 gtgcatagat gcccggggcg acgtgacctt ggaagaagac caaatcgcct tcttcgcctt
     6001 cgcctttggc tttccaaaag tggttgaaac cgacttcgta catggtggcg gcagattgga
     6061 aagatgcgat gtgcccaccc agttccaaat ctttcttgcc ggcgcgcaat acgatggcgg
     6121 cggcgttcca gcgcacgaat gcgcggatgc ggtgttcgat gttttggtcg cccggaatgc
     6181 ctttttcgtt ttcaaccgaa acggtattca aatacggggt ggtcgtgccg tgtggcatac
     6241 gtacgccctt gtcgcggcag tatttgacca ggttttccaa gaggtattgc gcgcgttcgc
     6301 cgccttcata ttcgaggacg gagcttaaag cgtccagcca ctcttgggtt tcgatagggt
     6361 caacatcgtg taattgggtg gacatagtat ctatccttta tgttgagtgt ttaatcggca
     6421 gggagggtgt agccaacccc tttattttcg tttgtgaaaa ataataagca aaaacgaaaa
     6481 tttctaaatc aaaccgaaaa aaataattta agtttaatgt tttcaaaaaa ttataaaagt
     6541 tttggaaaca agggaaaact tgaaaaaaat gttcgatctg ctccacacga aatcctatca
     6601 gcaatcgggt ctgctggcaa ataaccatgc ctgtaaaagc aggttttata ctgaagttgg
     6661 cgtttggtaa ggtcaagtct ttttgatttc gaaatattct gtttgatttt ggcgatattt
     6721 tttcggaatt gtggtatatc gaatcattat gtctctgtcg gcaattttcc tattgtagaa
     6781 aatctataca caagctttgt ggataaccgt gttgataagc cacggcttgt gttaaaaaag
     6841 tcttacgcaa caaatgtacg cttattttga ttaaaaaata ggcacattta aatctataaa
     6901 aatcatgtgc ttatgttcaa tatgatgttt ctggtcatgt ttttgccgtc tgttatggga
     6961 tttgtcccgc gcttggggca gggggtgaat tttggaggtt ggtgcaaaac ctgctttttt
     7021 cgcgttacaa tctgcacccg atttactttt gaatttgcct gtttggaaaa atacttttat
     7081 gaaagccagc caattcttta tctctacttt aaaagaagcc cctgccgaag ccgcgcttgc
     7141 cagccacaag ctgatgattc gcgccggcct gattaaagcc aacgcgtccg gcctttatac
     7201 ttggatgccg atggggctgc gcgtgttacg caaagtcgaa aacgtcgtgc gcgaggaaat
     7261 ggcgcgcgcg ggcagcgtgg agctgctgat gccggtggtg cagcctgccg agctgtggca
     7321 ggaatccggc cgctgggaat tttacggtaa agaactgctg cgcctgaaag accgccacga
     7381 ccgcgatttc tgcatgggcc cgacctgcga ggaagtcatc gccgacatcg tgcgcaaaga
     7441 aatcaacagc tacaaacagc tgccgaaaaa cttttaccac atccaaacca aattccgcga
     7501 cgaagtgcgc ccgcgtttcg gcgtgatgcg cgcgcgcgag ttcgtcatga aagacgctta
     7561 ttccttccat gccgactacg cctcgcttca gacgacctat gatgccatgt atgacgctta
     7621 ctgccgtatc ttcacccgtc tgggcttggc attccgcccc gtcgccgcag acaccggcag
     7681 catcggcggt acgggttcgc acgagtttca agtgttggcg gaaagcggcg aagatgttat
     7741 tgcgtacagc gacacttccg attacgccgc caatatcgag ttggcaccga ccttgccgct
     7801 taaaggcgaa cgcgccgctg ctcaggctgt gttgaccaaa gtacatacac caaacgtcaa
     7861 aaccattgag tctttggttg aattcctgaa tattccggtt gaacaaaccc tcaaatccat
     7921 cgtggtcgaa ggcgaaaacg aaggcgaaat cgtcttatta ctgttgcgcg gcgaccatga
     7981 gttcaacgac atcaaggcag aaaaactggc gggcgtgaaa tcgccattga cgatggcaag
     8041 ccctgccgcg attgtcgaac agttcggcgc aaacggcggc tctcttggtc ctgtcggctt
     8101 caaaggcaaa gtctatgccg atttcgctac cgaaaaaggc gcggactggg tcatcggtgc
     8161 gaacgaagac ggctaccact ataccggctt caacttcggt cgcgatgccg ccgagcctga
     8221 gtttgtcgat ttgcgcaacg tcgtcgaagg cgacgaaagc cccgacggac aaggccgtct
     8281 gaaactggcg cgcggcattg aagtcggaca cgtcttccag ttgcgcgaca aatatactaa
     8341 agccatgaac gtcagcttcc tcgacaacaa cggcaaatcg caaattatgg aaatgggctg
     8401 ctacggcatc ggcatcaccc gcgtcgttgc cgccgccatc gagcagaata acgacgaaaa
     8461 aggcatcatc tggaccaaag caatggcgcc gtttgaagtc gttatcgtgc cgatgaacta
     8521 caaaaaatca gacaccgtac gcgaagccgc cgacaaaatc tatgccgaat tgctggcggc
     8581 aggcgcggat gtgctgctgg atgaccgcga cgaacgcgca ggcgtattgc tgaacgattc
     8641 cgagctgttg ggtattccgc accgcatcgt catcggcgac cgcgctttga aagaaggcaa
     8701 tgtcgaatac gccgaacgcc gcaacaacga agcgcaggca gttgcaatcg gagaaattgt
     8761 tgcgcgtgta acagcttcat taaatgggta agcttggtca aatgccgtct gaaaataatt
     8821 tcagacggca tttttatttg gggagaaagt gaagtggctt cagtattttt agaaggagaa
     8881 gcagtcgagg tcggcaatat tttctgtatc ggcaggaact atgccgcaca tattgaagag
     8941 ttaaaaaacg aaataccttc cgaacctgtg gtgtttatga agccgtcagg cagcattttg
     9001 aatagcggcg gtacaatctt gctgcccgag tttagtaggg atgtgcagtt tgagtgcgaa
     9061 ctcgtgctgc tggtcggtaa ggattcagac ggcatgggag agggtgaaga tattttggga
     9121 tgtgtggcgg ggtacggcgt gggtttggat ttgaccgcgc gagacatcca atgccatctg
     9181 aaggcgcagg ggctgccctg gctgaaggca aaagggttca ggcattccgc gtgcgtgtcg
     9241 gattttgcgg cggcagacag aatcggcaat cccgagaagg tgttgttttc attaaaacaa
     9301 aacggcgtat tgaaacagcg cggggatacc ggtttgatga tttacccgat tcgggaaatt
     9361 ttacacaaac tggcggcgga ttacggtttg gggaaagggg atttggtgtt taccggaacg
     9421 ccctccggcg tgggcgcaat cggtgcgggg gacaatttgg cattggagct ggacggcttg
     9481 gtgcgcgcct ctttcacggt agggtgttaa ccgcgccgct taaccctcac gcccgttgaa
     9541 acattccgcc ggaccgccct cgaaaaaacc gtgcaagatg gcttgcgccg ccacttggtc
     9601 gagcaccgat ttgcgttttt tgccgaagac ctgtgcttcc gaaagcaggc tttcggcata
     9661 gacggacgac agccgttcgt caacccaata gacggggaga ttgaacctgc cgttcagcct
     9721 gcgtccgaac ttgcgcgaca ggtgcgtcat ttcatgtttc gtgccgtcgg tatgcacggg
     9781 cagtccgacg acaaaataac gcggctgcca ttcttgaacc agcttggcga ttgccgcgaa
     9841 cttttcatcg ttgctgccgc cggtaacggt gctcaaagga tgggatagcc ctaattccgc
     9901 gtctccttgt gccacgccga tacgcgcttc gccgaagtcg aatgccagtg ccgttccttt
     9961 tggaatttta tgcatggccg gctcccgaaa acagggcgag cgggtcgatg ccgagtttgg
    10021 cgaatgcggc ggcgtaacgg tgttcgtagg ggatgtcgaa caggatgtgt tcgtcggcgg
    10081 gaacagtcag ccacgcattg tcggcaagtt cgcgttcgag ctggcctttt ccccagcttg
    10141 aatagcctat gctgatcaag gctttgtcaa ccgcaccttc gcgtgaaata ttttcaatca
    10201 cgtctcggga agaagttagc gcgatattgt ctgaaacgcc gatactgctt tgccagttgc
    10261 cgatcggggt atgcacgaca taacctcgct cgacttgcac cggtccgccc atcatcacgc
    10321 tgtcgtgctg catccgcatg gggatgtttt tgccggtggc ggaaaaaatc atgtccatcg
    10381 taatcggaga gggtttgttg atggcgatgc cgagtgcgcc gtcttcatcg tgtttgcaga
    10441 tatagacgac cgattgtgaa aaaaacgcgt cttccatatc gggcatggcg acgagaaagt
    10501 ggttggagag gttcatatca gcgtttgtcg ggattttcta tcgggttgga aatcatgcag
    10561 gcgccggcaa gcaggttgcc ggaagtatcg gcttgcaccc catcggtttg cggtatgccg
    10621 ccgccgaaac aggaaaccgc gcttaaggaa tttttcggcg agccttggat gattttatcg
    10681 ctgtacacca aataggcgaa ggttttgcgt ttggggtcgt aataacggac aatctgccgg
    10741 ctcttgaacg cgaagctcgc accgtgtttg aaaacttctt tcggtttgcg cacggcggtt
    10801 tcgtcaaaag aaatcgaaga tgccgtctga acgcacgaaa ccgatgcgtc ggacgcgtcc
    10861 tcttccaaat tgaccatttc cttcaagccg cctttttttg catacgaaat ataacaggca
    10921 accccttgaa cgtcgggatc gtcgaatcct tccacttcga tacggtcgtt tttgcccagt
    10981 atgttgaaaa cggtacttgc ccgtccgatt ttatcggttt cgccgctgcc gcaggcagtc
    11041 agcaggacgg cggcagacag cagtagcagt ctgttcatca aaatttcctt atcggttgaa
    11101 tccccgtttt ccgtgaaggc ggcagggctt gctttgtttg gaggggcgca cgttcccaaa
    11161 atcgggcaac atacggtatg cggcttaatg tgctaccctg tgtgtcggaa cacaatatcc
    11221 gttgcggcat tgaaaatatg acgcacaata taaggctgtt tgagccgatt gcaagtatcc
    11281 gcgcaaagaa ggcgcaagat tgggcaaacg gcattttcag acggcataac tgacagtata
    11341 atccaaacgt ccggctgctt tcgtgcagcc ttcagtatta tccaactttt tccgaaggtc
    11401 gaaccatgcg ctacaaaccc cttctgcttg ccctgatgct cgttttttcc acgcccgccg
    11461 ttgccgccca cgacgcggca cacaaccgtt ccgccgaagt gaaaaaacag acgaagaaca
    11521 aaaaagaaca gcccgaagcg gcggaaggca aaaaagaaaa aggcaaaaat ggcgcagtga
    11581 aagataaaaa aacaggcggc aaagaggcgg caaaagaggg caaagagtcc aaaaaaaccg
    11641 ccaaaaaccg caaagaagca gagaaggagg cgacatccag gcagtctgcg cgcaaaggac
    11701 gcgaagggga taagaaatcg aaggcggaac acaaaaaggc acatggcaag cccgtgtccg
    11761 gatccaaaga aaaaaacgca aaaacacagc ctgaaaacaa acaaggcaaa aaagaggcaa
    11821 aaggacaggg caatccgcgc aagggcggca aggcggaaaa agacactgtt tctgcaaata
    11881 aaaaagtccg ttccgacaag aacggcaaag cagtgaaaca ggacaaaaaa tacagggaag
    11941 agaaaaatgc caaaaccgat tccgacgaat tgaaagccgc cgttgccgct gccaccaatg
    12001 atgtcgaaaa caaaaaagcc ctgctcaaac aaagcgaagg aatgctgctt catgtcagca
    12061 attccctcaa acagcttcag gaagagcgta tccgccaaga acgtatccgc caagagcgta
    12121 tccgtcaggc gcgcggcaac cttgcttccg tcaaccgcaa acagcgcgag gcttgggaca
    12181 agttccaaaa actcaatacc gagctgaacc gtttgaaaac ggaagtcgcc gctacgaaag
    12241 cgcagatttc ccgtttcgta tcggggaact ataaaaacag ccagccgaat gcggttgccc
    12301 tgttcctgaa aaacgccgaa ccgggtcaga aaaaccgctt tttgcgttat acgcgttatg
    12361 taaacgcctc caatcgggaa gttgtcaagg atttggaaaa acagcagaag gctttggcgg
    12421 tacaagagca gaaaatcaac aatgagcttg cccgtttgaa gaaaattcag gcaaacgtgc
    12481 aatccctgct gaaaaaacag ggtgtaaccg atgcggcgga acagacggaa agccgcagac
    12541 agaatgccaa aatcgccaaa gatgcccgaa aactgctgga acagaaaggg aacgagcagc
    12601 agctgaacaa gctcttgagc aatttggaga agaaaaaggc cgaacaccgc attcaggatg
    12661 cggaagcaaa aagaaaattg gctgaagcca gactggcggc agccgaaaaa gccagaaaag
    12721 aagcggcgca gcagaaggct gaagcacgac gtgcggaaat gtccaacctg accgccgaag
    12781 acaggaacat ccaagcgcct tcggttatgg gtatcggcag tgccgacggt ttcagccgca
    12841 tgcaaggacg tttgaaaaaa ccggttgacg gtgtgccgac cggacttttc gggcagaacc
    12901 ggagcggcgg cgatgtttgg aaaggcgtgt tctattccac tgcaccggca acggttgaaa
    12961 gcattgcgcc gggaacggta agctatgcgg acgagttgga cggctacggc aaagtggtcg
    13021 tggtcgatca cggcgagaac tacatcagca tctatgccgg tttgagcgaa atttccgtcg
    13081 gcaagggtta tatggtcgcg gcaggaagca aaatcggctc gagcgggtcg ctgccggacg
    13141 gggaagaggg gctttacctg caaatacgtt atcaaggtca ggtattgaac ccttcgagct
    13201 ggatacgttg atcggattgc cgaaacggta ggctttatgc aaatcgtgcc gatatggtgc
    13261 gctgttccgt gaagtttgcc gtttgccgta cctgtatttt ccggtttttg gaaatcaaga
    13321 gagaaagtta aagaagaatg tcgaaacctg tttttaagaa aatcgcactt tatactttgg
    13381 gtgcaatcag cggcgtggcc gtcagtctgg cggtgcaggg ttttgccgcc gagaaggaca
    13441 ggcgggataa cgaagtcctg ccggtgcaat ccatccgcac aatggcggag gtttacggtc
    13501 aaatcaaggc aaactactat caggacaaac ccgatgccga tttgtttgaa ggtgcgatga
    13561 agggtatggt ggccggtttg gatccgcatt ccgaatatat ggataaaaaa ggttatgccg
    13621 agataaagga gtccaccagc ggcgaatttg gcggcttggg gatggaaatc gggcaggaag
    13681 acggatttgt caaagtggtt tcgccgattg aggacacgcc tgcggaacgg gcgggggtga
    13741 aaagcggcga tttcattgtg aaaatcgata atgtttcgac acgcggcatg acggtcagcg
    13801 aagcggtgaa gaaaatgcgg ggcaagccgg gtacgaagat tactttgacg ctgtcgcgca
    13861 aaaatgccga caagctgata gtcgtcaacc tgacccgtgc cattattaaa gtgaaaagcg
    13921 tccgccatca cctgatcgaa cccgattacg gctatatccg cgtgtcgcag ttccaagaga
    13981 agacggtcga aagcgtcaat accgccgcaa aagagctggt aaaggaaaat aaaggaaaac
    14041 cgctcaaggg gctggtgttg gatttgcgcg acgaccccgg cgggcttttg actggcgcgg
    14101 tcggcgtgtc ggcggcattt ctgccgtctg aagcagtcgt cgtcagcacc aagggacgcg
    14161 acggcaaaga ccgcatggta ctgaaagcca ttcctgaaga ttatgtgtac gggatgggcg
    14221 gcgattcgtt ggcgggcatt cctgccgagt tgaaaaccat accgatgacg gtattggtca
    14281 attccggttc ggcttccgcg tcggagattg tcgcaggtgc attgcaggat cataaacgcg
    14341 cggtcatcgt cggtacgcag agcttcggca aaggttcggt tcagactttg attcctttgt
    14401 ccaacggcag cgcggtcaag ctgacaacgg cactgtatta tacgccgaac gaccgttcta
    14461 ttcaggcgca ggggattgtt cccgatgtcg aagtaaaaga taaggaacgc atttttgaaa
    14521 gccgcgaggc ggatttggtc ggacacatcg gcaatccctt gggcggcgag gatgtgaacg
    14581 gtgaaaccct tgccgtgccg cttgaaaaag atgcggataa gcccgctgta aaagaaaaag
    14641 gtaaaaagaa aaaggacgag gatttgtctt caaggcggat tcccaaccct gccaaagacg
    14701 accagttgcg gaaagctttg gatttagtca agtcgcccga gcagtggcag aagtctttgg
    14761 ggctggcggc gaaaaagccg gtttcaaata aagataagaa agataaaaaa gataagaagt
    14821 aggttttgac gatgccgtct gaaaggattt cagacggcat attatgtttg aaaggacagg
    14881 ttatgagcga ttttttaccg gaatttgaac cgtgcgg
//
LOCUS       DAWFBO010000034        14911 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFBO010000034 DAWFBO010000000
VERSION     DAWFBO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.034"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(92..880)
                     /locus_tag="WGO87_000761"
     CDS             complement(92..880)
                     /locus_tag="WGO87_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0392145.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(882..1757)
                     /gene="bioH"
                     /locus_tag="WGO87_000762"
     CDS             complement(882..1757)
                     /gene="bioH"
                     /locus_tag="WGO87_000762"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0392146.1"
                     /translation="MRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKS
                     MPDAVKKVYLIHGWGANRHVFDDLMPRLPATWAVSAVDLPGHGDAPFVQPFDIEAAAD
                     AVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTAAEDYPEGLAAP
                     ALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLARCGTPSALQEALDAA
                     ERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSH
                     AEAFAALYRDFVEGV"
     gene            1852..2349
                     /locus_tag="WGO87_000763"
     CDS             1852..2349
                     /locus_tag="WGO87_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0392147.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWRTLPLHTVFRRHRPPQSTLKG
                     GERRRNIKNAFEIRTPPPKNCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="WGO87_000764"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="WGO87_000764"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0392148.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2889..3011
                     /locus_tag="WGO87_000765"
     CDS             2889..3011
                     /locus_tag="WGO87_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0392149.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="WGO87_000766"
     CDS             3008..3727
                     /locus_tag="WGO87_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0392150.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANCSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMIQTVLTAG"
     gene            3781..4107
                     /locus_tag="WGO87_000767"
     CDS             3781..4107
                     /locus_tag="WGO87_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0392151.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4189..4773
                     /gene="ruvA"
                     /locus_tag="WGO87_000768"
     CDS             4189..4773
                     /gene="ruvA"
                     /locus_tag="WGO87_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0392152.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVGTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            4773..4910
                     /locus_tag="WGO87_000769"
     CDS             4773..4910
                     /locus_tag="WGO87_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392153.1"
                     /translation="MPSEGGATFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4936..6792
                     /locus_tag="WGO87_000770"
     CDS             4936..6792
                     /locus_tag="WGO87_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0392154.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLYSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPQALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6797..7720
                     /gene="rsgA"
                     /locus_tag="WGO87_000771"
     CDS             6797..7720
                     /gene="rsgA"
                     /locus_tag="WGO87_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0392155.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            7982..8206
                     /locus_tag="WGO87_000772"
     CDS             7982..8206
                     /locus_tag="WGO87_000772"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0392156.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            8190..9086
                     /locus_tag="WGO87_000773"
     CDS             8190..9086
                     /locus_tag="WGO87_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0392157.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAV
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9195..9494)
                     /locus_tag="WGO87_000774"
     CDS             complement(9195..9494)
                     /locus_tag="WGO87_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0392158.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9606..11051)
                     /gene="nuoN"
                     /locus_tag="WGO87_000775"
     CDS             complement(9606..11051)
                     /gene="nuoN"
                     /locus_tag="WGO87_000775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0392159.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALMFTLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRAHPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11061..12557)
                     /locus_tag="WGO87_000776"
     CDS             complement(11061..12557)
                     /locus_tag="WGO87_000776"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0392160.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGSDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFTILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAQVAQSKM"
     gene            complement(12646..14670)
                     /gene="nuoL"
                     /locus_tag="WGO87_000777"
     CDS             complement(12646..14670)
                     /gene="nuoL"
                     /locus_tag="WGO87_000777"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0392161.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQTATIQ
                     LFPSVEWSLITVTCLLLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLFVTLPLILLAIPSVIIGYVAI
                     GPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(14671..>14911)
                     /locus_tag="WGO87_000778"
     CDS             complement(14671..>14911)
                     /locus_tag="WGO87_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEZ0392162.1"
                     /translation="NILFIGIVFLFIQIAAYFDLKLILENQKTLYLIPLLPPAANIIV
                     LETAYLKTGRSASMIAYGTIFSLIAISIYILLVQI"
BASE COUNT         3794 a         4439 c         3854 g         2824 t
ORIGIN      
        1 cactatacat cggcatttcc tttagttctg tcggttttca caaacaaatg ccgtctgaaa
       61 cttggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttaa cggcgtgtcc gtacactgtt tccagcgtaa
      181 tactcaaacc gccttcccgt tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacga catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac ggggtcgtaa aacccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cggggaaaag cgcgctgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttcgg taaaagtgtc gcgcccgaag caggtaaaaa
      481 acagcagccc gtccgtcttc aaggcgcgcg cccagttgtg cagcacagga aggatttgtt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcggc acacgcttcg ggcagcggcg
      601 cggtcgggga ttggcagtgt tgcaccacgc ccttacccgt aaacctttgc caaaaaccgc
      661 ccttgcgcac ggctgcggca gccgccaaaa aatccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atatcgtttc gccagcaggc tgcggctgat gtccgcatcc gccccggcaa
      781 gcaggatatg tttgggcgcg ttgcggacga gtgtcagccg ttggtcggca tgttcggcaa
      841 gatggcggtg aacctgccaa catttgtcct gcggattcat gtcaaacccc ttcgacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgca tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct gcctttcaaa tgccgatgca gatactcacc catacgcggc
     1021 ggtgtaatcg cgtctttgcc gccgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaacgcct cctgcagggc ggagggcgtg
     1141 ccgcagcgcg ccaaatcggg cagaatcctg ccgatgattt cggcggcatc aggcgtgtgc
     1201 agaagctgca attgtagaaa ctgtttgata tgtttggcat aatccgttcg gaacgcgccg
     1261 accattttgc ccagtgcagg cgcggcaagc ccttcgggat aatcttcggc agccgtcagc
     1321 cgtgcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcga ccaatccgcc gagcgaccag ccgagaatgt cggcgggcgt atcgatttgc
     1441 gcggcaacag cgtcggcggc tgcctcaata tcgaagggtt gcacgaaggg cgcgtcgccg
     1501 tgtccgggca aatcgacggc ggacaccgcc cacgttgcag gcaggcgcgg catcaaatcg
     1561 tcaaacacgt ggcggttcgc cccccaaccg tgtatcaggt aaactttttt gacggcatca
     1621 ggcatggatt ttctctcttg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc cggcgtttca gacggcatct gcgccggctg ccgcgacgat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcat gcacgttcaa
     1801 ggcggcgcgg tgtgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggct
     1861 tcgctgcatt acgaaccgcc cgtcagcaat atgatacacg cgctgaagca cttggctgat
     1921 ttgagcatgg tgcagccgct ggcagacctg atgatgcaga atccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgta ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagtgaaag catcgtcggg ctgctggctc aacgctacgg ttggcgcacg
     2101 ctgcccctgc ataccgtatt ccgccgccac cggccgccgc aaagcacgct caaaggcggc
     2161 gaacggcggc gaaatatcaa aaacgccttt gaaatccgca caccgccgcc gaaaaactgt
     2221 aatattttgt taatcgacga tgtctttacc accggcgcga cgctggacga attggcaaag
     2281 acgctgaaaa aatcgggcgc aaaccgaatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccaaccg gaaatccccc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg aattcgccag
     2581 cctgacggtg tacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcgccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc cgatgcagcc cggcagccgg agtatgaacc tttccaatac
     2821 cgttgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaagttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acggcatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caactgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggcaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaata ccaaaaacgg caaaagcgtc atcgtccgcg tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcagc caagggacgg cgcacgtcaa aatcgaacaa atcgtcccgg gcaaatccgc
     3481 accggttgcc gaaaacaaag acatctttat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gcttcgacat caagcccgaa
     3601 cctcgccatt gaaaaacgcc gttacgaata cgtcgtcaaa atgggtccgt ttgcctcgca
     3661 ggaacgcgcc gccgaggccg aagctcaggc gcgcggtatg attcagacgg tattgaccgc
     3721 cggttgatgt cccgccttta cgtctttcaa acaccaccag atacttcgca ggagcaaaac
     3781 atgaacttca aacgccttct cttgaccgcc gccgcaaccg cactgatggg catttccgcc
     3841 cccgcactcg cccaccacga cggacacggc gatgacgacc acggacacgc cgcacaccaa
     3901 cacagcaaac aagacaaaat catcagccgc gcccaagccg aaaaagcagc gttggcgcgt
     3961 gtcggcggca aaatcaccga catcgatctc gaacacgaca acggccgtcc gcactatgat
     4021 gtcgaaatcg tcaaaaacgg acaggaatac aaagtcgttg tcgatgcccg taccggccgc
     4081 gtgatttcct cccgccgcga cgactgaatt tgatacaatc cgtgccgtct gaagcccgaa
     4141 ccggtttcag acggcatttt gcacccgaca cttcaggatt cggcatacat gatcagcaga
     4201 ctgaccggca aactggttga aaaaaaccct ccgcaaatcg tcatcgatgt caacggtgtc
     4261 ggctatgagg ccgacgtatc gatgcagacc ttctacaacc tgccgcccgt gggtgaaagc
     4321 gtacaactgt ttacccagct tatcattcgg gaagacgcac atcttttatt tggttttgcc
     4381 actgcggaag aacgcaaaac attccgccaa ctgattaagg tcagcggcat cggcgcgaaa
     4441 acggctttgg gcattttgtc ggcgatgacg gcagacgagc tggcgcaggc ggttgcagaa
     4501 gaagatgtca aacgcctctc ctccgccccg ggaatcggca aaaaaaccgc cgaacgcatg
     4561 gttttggaac tgcgcggcaa gctggtcgcg catgcggtaa cggacgggct gtttgccgcc
     4621 gcacccgccg ccgacgaaac ggaagacatc gtcggcacgc tcctcgccct cggttacagc
     4681 gaacgcgagg caaaagcggc ggtcaaaggc gttccgaaag ggacggacgt gggcgaaggc
     4741 gtgcgccttg ctttgaaaaa cctgctgaaa taatgccgtc tgaaggcggc gcgacgtttg
     4801 ccctgacgaa accctgcgtt tccgcctcgc gctgtcttcc cgacggcttt tcccgtaaaa
     4861 tggcgttatt gtccttcctt tcagacggca ttgtgtttga tgccgtctga aactgtttta
     4921 ccgaaatcga aaacaatgtt gaacaaaata ttttcctggt tcgagtcccg aatcgaccct
     4981 tatcccgaag ccgccccgaa aacgccagaa aaaggcttgt ggcggtttgt ctggagcagc
     5041 atggccggcg tgcggaaatg gatagccgcc ctggctgcgc tgaccgccgg catcggcatt
     5101 atggaagccc tggtttttca atttatgggc aaaatcgtgg agtggctcgg caaatacgcg
     5161 cccgccgaac tgtttgccga aaaaggttgg gaactggcgg caatggcggc gatgatggtg
     5221 ttttcggtcg tgtgggcgta tgccgcgtcc aacgtgcgcc tgcaaaccct tcagggcgtg
     5281 ttccctatgc gcctgcgctg gaacttccac cgcctgatgc tgaaccaaag cctcggtttt
     5341 tatcaggacg aatttgccgg acgcgtgtcc gccaaagtca tgcagaccgc gctggcgttg
     5401 cgcgacgcgg tgatgacggt tgccgatatg gtcgtttatg tgtcggtgta tttcattacc
     5461 tccggcgtga ttctcgcctc gctctactca tggctgctgc tgccctttat cggctggatt
     5521 gtcggtttcg cttcggtgat gcgcctgctg attcccaaat tggggcaaac cgccgcatgg
     5581 caggcggatg cccgctcgct gatgaccggc cgcattaccg atgcctattc caatatcgcc
     5641 accgtcaaac tcttctccca cggcgcgcgt gaagccgcct atgccaagca gtcgatggaa
     5701 gaatttatgg ttacggtgcg cgcccaaatg cggctggcga cgctgctgca ttcgtgcagc
     5761 ttcatcgtca acacctccct gaccctctcc accgccgcac tgggcatctg gctctggcac
     5821 aacgggcagg tcggcgtggg cgcggttgct acagccaccg ccatggcgtt gcgcgtcaac
     5881 ggtttgtcgc aatacattat gtgggaatcc gcgcggctgt ttgaaaacat cggcaccgtc
     5941 ggcgacggca tggcaaccct gtccaaaccg cacaccatcc tcgacaagcc ccaagccctg
     6001 ccgctgaatg tgccgcaagg cgcaatcaaa ttcgaacaca tcgatttctg ctacgaagcc
     6061 ggcaaaccgc tgctcaacgg cttcaacctc accatccgcc cgggcgaaaa agtcggcttg
     6121 atcggacgca gcggcgcggg caaatccacc atcgtcaacc tgcttttgcg cttctacgaa
     6181 ccgcaaagcg gcacggtttc gatcgacggg caggacataa gcggcatcac ccaagaatct
     6241 ttacgcgccc aaatcggttt ggtcacgcaa gatacctcgc tgctgcaccg ttccgtgcgc
     6301 gacaacatta tttacggccg ccccgacgcg accgatgccg aaatggtttc cgccgccgaa
     6361 cgcgccgaag ccgccggctt catccccgaa ctttccgatg ccaaagggcg gcgcggctat
     6421 gacgcgcacg tcggcgaacg cggcgtgaaa ctctccggcg ggcaacgcca gcgcatcgcc
     6481 attgcccgcg tgatgctcaa agacgcgccc atcctgctgc tcgacgaagc caccagcgcg
     6541 ctcgattccg aagtcgaagc cgccatccag gaaagcctcg acaaaatgat ggacggcaaa
     6601 accgtcatcg ccatcgccca ccgcctctcc accatcgccg caatggacag gctcgtcgtc
     6661 ctcgacaaag gccgcatcat cgaagaaggc acacacgccg aactcctcga aaaacgcggg
     6721 ctttacgcca aactctgggc gcaccaaagc ggcggcttcc tcaacgaaca cgtcgagtgg
     6781 cagcacgact gaaccgatgc cgtccgaaca cccgttttca gacggcattt ccacacccaa
     6841 ccccaaagaa accatgaacg acaccgccca aattaccgcc agctacggcc gccgctacat
     6901 cgtccgcacg cccgacggca caacctacga agccagcacg cgcaaaaaac gcgtcgattt
     6961 cgcctgcggc gaccgtgtcc gcatcagccc cgtcaacgcc gaacaagttg tgattgaaga
     7021 ttttttaccg cgccaaagcc tgctctaccg ccaagacgcg tggaaaacca aactcatcgc
     7081 cgccaacgtt acccaactcc tcatcgtaac cgccgccgtc ccgagtccga gcatgcggct
     7141 gctgcaacgc gccctgcttg ccgccgaagc cgccggcatc cgcgccgtca tcgtcctgaa
     7201 caaagccgac ctgcccgaaa ccgccctttg gcgcgaaaaa ctcaaattct acgaaacgct
     7261 gggttatccc gtcatcgaaa cccgcgcact ggaaaacgcc ggcagcctgc gccccgccct
     7321 gcaagggcac agcaacatcc tgctcgggca gagcggtatg ggcaaatcca ccctgaccaa
     7381 cgcccttttg ggcagccaaa ccgcccgcac cggcgacatt tccgccgcac tcgactcggg
     7441 aaaacacacc accacccacg cccggcttta tgatttgaac ggcgaaaccc aactcatcga
     7501 ctccccgggt ttgcaagaat ttggtttaca ccacctccaa gccgccgatt tgccgcgcta
     7561 tttccccgat ttccgccacc ttgtcgggca atgccgcttc cacaactgca cccaccgcgc
     7621 cgaacccggc tgcgccttca aagccgccgc gcaaaccggg gcggcaagcc ccgaacgcct
     7681 cgcctttttg cagggcatca ccgacgaact gcccgggtaa cgccttgccg cctgggcgga
     7741 aaaaatgccg tctgaagccg gattcgggtt tcagacggca tccgtttttc aaaaatgcta
     7801 caatccgctt tttactggaa catccgaaac tatgttccaa cacgtaggac gacacttaaa
     7861 gcaccgccct atgtgtcgtc ctgatttgga agggcttaca cccctcccaa ataaagtctg
     7921 attctaccgc cccgaaggac agatgtccaa gtggcggggt ttcaaccgaa aaggaaacac
     7981 gatgaagaaa aacgccccga aatcgtttga agaagccttg tcgcgccttg aatcgctgac
     8041 acaagctatg cagggcgaaa tgcccttgga agacgcgctt gccgcctatc aggaaggcaa
     8101 cgagctggtc agatactgcc aaaccaagct ggcgcaagtc gaacagaaat tacaggtttt
     8161 ggacgcggac gggccgaagg agttgaacct tgaatccgac gaatgatttg aaagcgtggc
     8221 aacagagggc gcaggcgcaa acagagctgc tgctggaacg ctttttgccg tctgaaaacg
     8281 aaataccgca cacactgcac gaagccatgc gttatgcggt tttggatggg ggcaaacgtc
     8341 tgcggccgat gctggttctg gcagcttcgg aattaggcga agccgtgcat gaagcagtag
     8401 aacaggcaat ggcggcaatc gaaatgatcc atgcctattc gttggttcac gacgatatgc
     8461 cggcaatgga caacgacagc ctgcgtcggg gcaaaccgac ctgccacatc aaatatggcg
     8521 aagccaccgc gctactggtc ggcgacgctt tgcaaaccca agccttcgac gtgttaagcc
     8581 gtccgacaga actgcccgcc gcacgccagt tggcaatgtt gtcggtgttg gcgaaagcgg
     8641 gcggcagcgc gggcatggcg ggcggtcagg caatcgattt ggcgaatgtc ggcaaacaaa
     8701 tggttcaagc cgatttggaa cagatgcaca gcctgaaaac gggtgcgcta atccgtgcgg
     8761 cggttttatt gggggcgatg tcctgcccga atctgtctga agacagtctg gcggtattgg
     8821 acgattatgc ggcaaaactg gggctggcgt tccaagtcat tgacgatgtg ttggattgtg
     8881 aagcggacac ggcgacttta ggcaaaacgg cgggcaaaga cgcagacaac gacaagccga
     8941 cttatgtgaa actgatgggc ttggaagcgg cgcgctcata cgcacacaaa ctggttgccg
     9001 aagcggtcgc gctgctcgaa cccttcggcg acaaagccct gcgcctgcgg cagttggcag
     9061 aatttgcagt cgcccgcaaa tattaaaacc ggcgtatatc gccggctgaa acgtgccggc
     9121 acagcgatgc cggcctgcac ttgaatgaaa caaatgccgt ctgaacaggt ttcagacggc
     9181 attcctatgc ctgcctattc cctgttgcgc gtgtgccaaa aataccgcca cacgaaacag
     9241 ggaaacgcaa aaatcaggta caggcagacg acggtggcgt agaactccca accctgatcg
     9301 tgtaccgctc ccgcacggga ttcgaggatg taggcaagaa cggcggtcag cgcgaaacct
     9361 gccgccagct cgatcaagtg gtgcccgaaa tgtttgcgct tgagcggtac gatgccgaac
     9421 agcctggtcg tgaggaaggg ggcgttggca aaaatcaagg caagcagcaa aaggatgtac
     9481 atggatgcgg tcatggcggg ctttctgttt gggcattgtc ggcggcgatg ccgtctgaac
     9541 tgcgttgcgg gacggcatat cttaacaaaa acggcagcct ctgacacggc tgccgttgcg
     9601 gcggcttaca gcgtgttttc caacgccttg gcgcaccagt cgataacggt ttgcggcata
     9661 atgccccaca gcaccaacag cagcgcgttg acgctcaaaa ggaattttgc ggcattgttg
     9721 ccgccgacgg tgggatgggc gcggccggat tcgtcaaaat acatgacttt gaccacgcgc
     9781 aggtagtaga acgcgccaat cagcgacatc acgacggcaa atacggacaa ccaaacatag
     9841 ccttgtttta agagtgccat aatcacgcca aatttggcgt aaaaacccat cagcggcgga
     9901 atgcccgcca tagagaacat aaccagtaac atcaaaaagg caagccatac gcggtgttgg
     9961 ttcagccctg ccaaatcgct gatgttttcg cactcgttgt ccccatccga caacaccatc
    10021 aacacgccga accctgccgc cgccatcagc gcgtaggtaa tggcgtaata gagacccgcc
    10081 gcaaagccga ccgcgcccgc cataaacgcc agcaggataa accccatatg cgatacggtg
    10141 gaataggcga gcatacgttt gatattggtc tgcatgatgg cggcaaggtt gccgaccagc
    10201 agcgaggcgg cggcaagcag ggcaaacatc agagaccagt catgatgcac ggttcccaac
    10261 ccggtaacga ggatgcggaa agcgaaaacg acggcggcga ttttcggggc agtgccgact
    10321 agggcggtaa cagaagtggg cgcaccgtga tacacgtcgg gcacccacat atggaacggc
    10381 accgcgccga gtttgaacgc aacggcgacg acgataaaca ccagacccag tttcaacagc
    10441 cattcgtttg cttcttcatt gaaggaagag gcgagcacgc cggcaaattc cagcgaaccg
    10501 gttgcgccgt aaaccataga aataccgtag agtagcagac cggatgccag cgcgcccaga
    10561 acaaaatatt tcaaggcggc ttcggcggca aagccggaat cgcggcgcag ggcaatcagg
    10621 gcgtaaaggg caagcgacaa gagttccaaa ccgatatagg cagttaaaaa atgccccgcg
    10681 ctcaccatca cgctcatgcc caagagggca aacagcgaca gggtgtaaaa ctcgccttta
    10741 aaaataccgc gcacttggtt gtagggcttg gcatagacaa acagggtaaa catcaaggca
    10801 tataaaacca tttttgccaa acgcgacata ccgtccgcaa tatacatccc gttgaacgaa
    10861 gacgtgctgc cctgttccca cacggcaagc tgcaccgccg ccgtaaccgc caccgttgcc
    10921 aacgcgccgt aatgcgtcca acggcgtttg tcatcactga cccacaagtc tgccagcaac
    10981 aataacacca gcagcgacag cagcgcgact tcgggcagtg cgggcatcaa attcaaatca
    11041 gaccaattca tttacacacc tcacatcttg ctttgcgcca cttgggcaat caaatcgttt
    11101 gccgcctgat gcaccacttc gataaatgcg ttcggataca ggcccatacc caataccgca
    11161 accgccaaaa ccgccagaat cgtaaactcg cgacagttga tgtctttcat ttcggcaacg
    11221 tgcggattgt gaatcgcgcc gaaaatcacg cgcttgtaca tccacagggt ataagacgca
    11281 ccgtaaatca gcgtcatagc cgccaacgcg cccacccaga aattcacttt aaccgcgccc
    11341 ataatcacca taaactcgcc cacgaagcca gaagtcgccg gcagaccggc attggccata
    11401 ccgaacaaca tcataaacgc cgcaaactta ggcatcacat tgaccacgcc gccatagtca
    11461 gcaatattgc gggtatgcag gcggtcgtac atcacgccga tacacataaa catcgccgcc
    11521 gacacgaaac cgtgcgaaat catttgaatg attgcgcctt tcaacgccca gtcgtccaac
    11581 tgcccgtcaa caaacaaaaa catcccaagc gttacaaaac ccatatggct gatagacgaa
    11641 tacgccacca gttttttcat atcggtttgc accaaagcca ccataccgat ataaatcacg
    11701 gcaatcaggc tcaatacgat gatcacaggg gcaaaatagc gtgccgcatc cggcataatc
    11761 ggcaggataa aacgcaagaa accatacgca cccagtttca gcgtaattgc cgccaacacc
    11821 atcgaaccgc cggtcggcgc ttcaacgtgg gcatccggca accaagtgtg cacagggaac
    11881 atcggcactt ttacggcaaa tgacaggaag aatgccacaa acaaaagctg ttgtacgccc
    11941 aatggaatct gcttgatgtt ttggaaatcg acaatagaga agctgcctgt ctgatagtac
    12001 aggtaaacca tggcaaccag catcaaaagc gaacccatca gcgtatagag gaacagcttg
    12061 accgacgcat aaacgcggcg cgggccaccc catacaccga taatcaggta cagcggaatc
    12121 agcataccct cgaagaacac ataaaacaga atcgcatcct gtgcggcaaa cgcgccgtta
    12181 atcaaacccg acatcatcag gaatgccgcc atatactgcg ccggacgttt ctgaataact
    12241 tcccaaccgg ccaataccac caacagcgta ataaacgcat tcaagatgat aaagagcact
    12301 gaaataccgt ccacgcccaa tgcatagttg attttcagca gcggaatcca ctcgtggaac
    12361 tcggtaaatt gatagccgcc gctcaaacgg tcgaaaccgg taaacagggg cagcgttacc
    12421 aagaaaccgg caagcgcacc catgaaggca agcacacgcg ccagcggcgc acggctgtcc
    12481 gaccccgttg ccaaaaccag cacacctgcg gcgatgggta tccatattgc caagctgagt
    12541 aggtagttgg aaaacatagt ggttaacctg tggttaaaat aaaaatatcg ttgtacgggt
    12601 tggtttgtca gacgacgttt taagagaccg tctgaagcct catccttatc tgaacaatcc
    12661 ccagaaggtc atgccgagca gtaccaatac gccgaacacc atagcggcgg cgtaggtgta
    12721 gataaagccg gtttgggctt tacgcacttg cgcggcaatc gcgccgacca gtttggcaga
    12781 gccgttgacg atgccgttat cgataatggc ggtatcgccg actttccaga agaaggtacc
    12841 cagtgcgcgc gtgcctttag cgaagacgtt gaaatacagg gcgtcgaggt agtatttgtt
    12901 ttcaaacaaa acgtaaaccg gacggaacgc ctgtgcaatt ttcgcaggca ggtgcggcag
    12961 tttgacgtac aaaagccatg cgctcaatac acctgcgata gcgaggtaga gtacaggaga
    13021 atgcaggctg tgcgacacca ttgccaatgc gccgtggaac tcttccttca tgatgtgcat
    13081 ggtcggatgc gcgtcggtat tgacgaaaat cacgtctttg aagaaatcgc cgtaaagcat
    13141 aggcccgatg gcaacatagc cgatgatgac ggacggaatg gcaagcaaaa tcaaaggcag
    13201 ggtaacgaac agcgggcttt cgtgcggatt gtcgtttttg cccaagccgt gatgttcttc
    13261 gccatgtccg tctgaatggt gttcgggcag gctgcgccat ttctcttcgc cgtggaacac
    13321 cataaagtat tggcggaacg cgtaaaacgc ggtaacgaac acgctggcaa ggacggcaaa
    13381 ataggcaaag ccgctgccgg gaagggtgct gtatttcgcc gcttcgataa tcgaatcttt
    13441 ggagtaaaag ccggagaaga acggcgtacc aatcagcgac aagttaccga tcaacatggt
    13501 caaccaagta atcggcatgt attttttcag gttgcccatg tggcgcatgt cttggtcgtg
    13561 atgcataccg ataatcgcgc tacctgcggc caagaacaac aaggctttaa agaaagcgtg
    13621 ggtcatcaca tggaacatcg ccacggaata ggcagacgcg cccagagcca cagtcatata
    13681 gcccagttgc gacagggtgg aatacgcaac cacgcgcttg atgtcgtttt gaatcacgcc
    13741 caagaaaccc ataaacaggg cggtaatcgc accgattacc atgatgacag acagcgcagt
    13801 gctgctcatc tcataaatcg gcgacatacg cgataccatg aacagaccgg cggtaaccat
    13861 ggttgcggcg tgaatcaatg cagaaatcgg ggtcgggcct tccatcgaat caggcagcca
    13921 gacgtgcagc gggaattgtg ccgatttacc catcgcaccg ataaacagga gcaaacaggt
    13981 tacagtaatc aaagaccatt ccacactggg gaacaattga atggtggccg tctgaacatt
    14041 aggcagataa gcgaatacat cttgatagcg caggctgccg ccgaaatagg caagcaccaa
    14101 gccgataccg agcaaaaagc cgaagtcgcc gacacggttg atcaaaaagg ctttcaggtt
    14161 ggcaaatgtc gcgctcggac gtttgaaata gaaaccgatc aagagatacg acaccaagcc
    14221 caccgcttcc caaccgaaga agagctggat gaagttgttg ctcataatca gcatcaacat
    14281 actgaaagta aacaaagaaa tatagctgaa gaagcgttgg taaccgactt tttcatcgtg
    14341 catatagccg atggtgtaga tatgcaccat caacgacacg cccgttacca cgaccatcat
    14401 catcgccgtc atcgtatcga ccaagaagcc gacggaaaaa tccaagccgc ccattgtcag
    14461 ccaggtataa acgttctcgt caaacttggc acggctgccg tcaataaagc cccacaacac
    14521 ataagccgac agcacggcag acaccgccac accgagtatc gtaaccgtat gcgcgccggc
    14581 acgtccgatt ttgttgccga ataaacccgc aatcagcgag cctgccaaag gcacgagggc
    14641 gatgatgaga tataaagtca tgtcgttcat ttaaatttgc accagtaaaa tataaatgct
    14701 gattgcgatt aaactaaaaa tcgtaccata cgcaatcata gaagccgaac gccctgtttt
    14761 caaatatgcc gtttccaaca caataatatt ggcagcaggc ggcagcaacg gtattaaata
    14821 caatgttttt tggttttcaa gaatcaactt caagtcaaaa tatgcagcta tttggataaa
    14881 aagaaaaaca atccctataa ataaaatgtt t
//
LOCUS       DAWFBO010000035        14876 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFBO010000035 DAWFBO010000000
VERSION     DAWFBO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.035"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1..682)
                     /locus_tag="WGO87_000779"
                     /pseudo
     CDS             complement(1..682)
                     /locus_tag="WGO87_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(768..1190)
                     /locus_tag="WGO87_000780"
     CDS             complement(768..1190)
                     /locus_tag="WGO87_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392163.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITPGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            1502..2812
                     /locus_tag="WGO87_000781"
     CDS             1502..2812
                     /locus_tag="WGO87_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0392164.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDGLKKLVAKVLALPEYRDFTIEADAQELFVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            2926..5931
                     /locus_tag="WGO87_000782"
     CDS             2926..5931
                     /locus_tag="WGO87_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0392165.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKHGIVPKFYFIVDRLDLLKQAQREFTARDLIVHTIDSREAFAADIKSAQTLHN
                     HSGKPEITVVNIQKFKDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     INAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSIDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDDSLGAMVVC
                     DSSEQARQLFEHFQTASNHNLTAVLILHDIGTKEERDQWVKDFKAGKIDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIQSEFDKTNRAY
                     WDELSNELGDEIGNYSQLFKTTEEIEQEIAGIKDALFEFDRENAEVFRHQIEQIADKK
                     QLLALKKALQTARELYNVLRLQGSHEFLECLDFDKLNLLYREAVSRLDTLNIIEKQQQ
                     GNIAHLLNEALEDIYFQFVKISEAELKLADDLKDIMRKVREGLAGNIDQDDPEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLDTVYAQIKELNRKNDLLRHKYGGDTKYARIH
                     KRLLENPALYGDKLKVFNALNGVKTDADQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            6061..7296
                     /locus_tag="WGO87_000783"
     CDS             6061..7296
                     /locus_tag="WGO87_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0392166.1"
                     /translation="MIEKRLIDISKNISSGITPLRSNDEFWTDGTIPWLKTEQLGEKY
                     IFDTNEYITEKALQEANVKIFPENTLSIAMYGEGKTRGNVSILKRPMATNQACCNIEL
                     DEEKVSSEYVYYFLKTQYENLRGLSSGIRKNLNTNDIKNFVVRLPENLKTQQSIAAVL
                     SALDKKIALNKQINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGEMVFDETLKRE
                     IPKGWEVKSLWKIAKYFNGLALQKYRPENELDDFLPVIKIREMNEGVSSNTERAKTNI
                     PKEAIIDDGDILFSWSATLEIKIWSQGKGALNQHIFKVTSSEYPKYFFYFELLNYLKH
                     FKMIADLRKTTMGHITQDHLKQAYICIPSQPLLEKLEKIVTPIFQKILITQKQNHQLT
                     QLRDFLLPMLMNGQVSVAE"
     gene            7345..8946
                     /locus_tag="WGO87_000784"
     CDS             7345..8946
                     /locus_tag="WGO87_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0392167.1"
                     /translation="MTEQHFTEQTKALIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKENPDEEIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEV
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPYHKDASGRLKKFDFIVSNPPFKLDFSDFRDQLESDENRERFFAGIPKIKAKD
                     KDKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKANKDNVVLIDASGLGEKIKDGKNQKTVLSREEEQKICN
                     TFTHKQAVEDFSVVVGYDEIKAKNYSLSAGQYFEVKIDYVDISAEEFEQKMAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(9042..10379)
                     /gene="ribB"
                     /locus_tag="WGO87_000785"
     CDS             complement(9042..10379)
                     /gene="ribB"
                     /locus_tag="WGO87_000785"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0392168.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(10593..11606)
                     /locus_tag="WGO87_000786"
     CDS             complement(10593..11606)
                     /locus_tag="WGO87_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0392169.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFTDYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     GGGILFDLQQLADKKVDLILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYIANA
                     WTKIVRREIIIKNNLFFPKGYIHEDFPYNLQLARFIKTFAFYDNPFYQYRVLGGSISH
                     NIKYKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNII
                     VIYRKYFSFKEKCRKIFGAKAIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKF
                     FSE"
     gene            complement(11762..12355)
                     /gene="ribA"
                     /locus_tag="WGO87_000787"
     CDS             complement(11762..12355)
                     /gene="ribA"
                     /locus_tag="WGO87_000787"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0392170.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(12348..13025)
                     /locus_tag="WGO87_000788"
     CDS             complement(12348..13025)
                     /locus_tag="WGO87_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392171.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEDWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            13179..13263
                     /locus_tag="WGO87_000789"
     tRNA            13179..13263
                     /locus_tag="WGO87_000789"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13213..13215,aa:Leu,seq:tag)
     gene            13783..14817
                     /gene="purM"
                     /locus_tag="WGO87_000790"
     CDS             13783..14817
                     /gene="purM"
                     /locus_tag="WGO87_000790"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0392172.1"
                     /translation="MSISLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         4030 a         3581 c         3543 g         3722 t
ORIGIN      
        1 cgcaaatgcc gttttgtaca attccaaaag ttttcaattc cgttaatgcg attttgccgt
       61 ttggcgaaat gcgtactgtt ccagtcgtgg attgaacccc caccctgtat agttctttcg
      121 aagcattggg gtattgtttt ttcaaagcat cgattaaagc cgtattggtg tccggatttt
      181 taacctggac aatattgtcc ccttcggctt caagcgttgc ttttgcagaa tgcgacgcga
      241 taatgccttt tacggaggcg tggtttttta catttaaatc aacttcatta tttcccttga
      301 cggctttgag cttgatagtg ctgatgccat caagggaagg ttgggtaagg tcgcttgatt
      361 gattattgat gaaaatgccc gtcgtgcgtc cggaataggg ggagttggac tggagaccat
      421 ttacgcccaa tcccaagccc ttgccctcag tggcgatgga tattttattg ttattccccg
      481 ttaatacggt attggattta aaagccgttt caatgcccct taaaaaactt gtactctcaa
      541 catttcctgt cggcataggg tcggcgagtt cccctttaat ctcaatcaca ttatcgctgt
      601 cttcggcagt cagatgaaca tcggtattgt tgaggttgtg aataccgaaa tggtgtgtgt
      661 ctttatgaac gttaacgttc atattgaaca aattgcctgt ttttgaatga aaggatatgc
      721 tgcctttgtc attttcatcg gggggctgat agtgttgaca taccgcacta aggcactatt
      781 gtttgtcggt atttttttcc actggctaca tcttgtaccc catcaacatc gggaacattc
      841 aaattaaatt gaatttttcc tttggcatcc aaatggatat ttccggaatg gggcttcacg
      901 tcaacagatt ggagattatt tttaaataga atatcattat tgctttttac caataattca
      961 ggcgtatatc ccaattgaga gtctgcaaaa gattggatgc cgtaaattcc atttaccgtt
     1021 aaatttccat tatcattttt accaggggta ataagttcta attttctaaa gttttcacct
     1081 tttttccacc aaggcacaat atagtttttt acagtgattt tttcatcttt aagggttata
     1141 agactatcat cgtctctttg cggatcagaa aataaagaca cccattccat ctcttgaata
     1201 ttagtatatt catgggcttc cgcctcaaaa cataatgccg attgaatcaa caaagcaatg
     1261 gcagataaag caaattttct attttagtct ccttaatgtt taattgaaac gctgatttgg
     1321 gtattgtcat gccgatgcgg aatcggtctc cgtgccggat atattgtgtg aagtctctcg
     1381 tcttttccag tatgcgccta atatcggctt aaggcaaacg gaggggcgga atccgtatgc
     1441 ggcacgccgc cgttccctgc cgtataatca tattgtttga aaacctgttg acggagccgt
     1501 tatgaccgat ttgtttgccc gagaacccga cgcgccgctt gccgaacgat tgcgtccgca
     1561 tacgcttgac gacgtggtgg ggcaggaaca cctcatcggc gaaggtaaac ctttgcgcgt
     1621 ggcggtagaa ggcggcaagc cgcattctat gttgctgtgg gggccgccgg gcgtgggcaa
     1681 gacgacgttg gcgcggattt tggcgcagag tttcaatgcc cagtttttgc ctgtttccgc
     1741 cgtgttttcc ggcgtgaagg acatccgcga ggcaatcgat aaagccgaaa tcgctttgca
     1801 gcagggacgc gcgacgattt tgtttgtcga tgaagtccac cgcttcaaca aggcgcagca
     1861 ggacgcgttt ttgccgcatg tcgaaagcgg tttgctaacc tttattggtg cgacgacgga
     1921 aaatccgtcg tttgaagtca atcccgcgct gttgagccgc gcgcaggtgt atgttttgca
     1981 gcccttgtct tcagacggcc tcaaaaagct ggttgccaaa gttttggctt tgcccgaata
     2041 ccgggatttc acgattgaag ccgacgcgca ggagctgttc gtcaataccg ccgacggtga
     2101 tgcgcgcaga ttgttgaatt tgttggaaca acttttacgc gccgccgata cacgtcgtct
     2161 gaaaaactta accgccgaat ttctcgccga cagtctcggg gcgcaaatcc gccgtttcga
     2221 caaaggcggc gagagcttct acaaccaaat ctccgccctg cacaaatccg tgcgcggttc
     2281 gcatccgaac gccgcgctgt attggttctg ccgtatgctc gacggcggca ccgacccgcg
     2341 ctacctcgcc cgccgcatcg tgcgtatcgc ttgggaagac atcgggcttg ccgacccgcg
     2401 cgccttccaa atcgccaacg atgccgccgc caccttcgaa cgcttaggct cgcccgaagg
     2461 cgaactggct ttggctcaag ccgtgctgta ccttgctgcc gccgccaaat ccaacgcggg
     2521 ctacaaggca tacaaccaaa tgcgccactt cgtcaaagaa aacgccagcg acgaagtgcc
     2581 cgtccacctg cgcaacgccc cgaccaagct gatgaaggaa ttgggctacg gacgcgaata
     2641 ccgctacgcc cacgacgaac cgaacgccta cgccgccggc gaaagctata tgcccgacgg
     2701 tttggacgaa ccggacttct accaacccgt cccgcgcggg ttggaaatca aaatcggcga
     2761 aaagctggaa tggttgaaat cgctggacga agaagtatta aaggcaaaat gaagcaatgc
     2821 tgtctgaagc agaacagtat ggcaaagccg gtacaccgaa aaggcgtatc gggtttttca
     2881 ggtgttataa tccgtttcaa taaattttga tgaatcatta cgtttatgtc ccaaactcac
     2941 aacgaaaatt cccgcgtcaa aatccccgcc gttttgcacc tgatgcgctt gggctacgac
     3001 tacctctcgc tcaaaaacga aaattgggat aaaccaacca acatctttcc caaaatcttt
     3061 atagaaagcc tctgccgcat caatcccgat ttgtcgccgg acgatgcacg ccgtttgctg
     3121 accgacatac gcttagaact ggacaacgaa gacttggggc aaaagtttta cgaacgcctt
     3181 accaatcaat ccggcggcaa gaaactgatc gacttccaaa attttgataa caacagcttc
     3241 cacgttgtaa ccgaattgcc ctgtatcaac ggcgatgaag aattccgccc cgacattacc
     3301 ctgctggtaa acggcatgcc tttggttttt atcgaagtca aaaagcccaa caacaaaggc
     3361 ggaatcggcg aagagcggga gcgcatgggc aaacgtgcga aaaattccaa atttcgccgt
     3421 ttcatcaaca tcacccaatt catgattttt tccaacaaca tggaatacga cgacggcgca
     3481 accgaaccgg cacaaggcgc gttttacgct tcgtctgctt acggtaagcc cgtgtttaac
     3541 tacttccgcg aagaacataa attaaatctt gccgaattat tgaacacgct ttcagacggc
     3601 ctcgaaaaca acgtcctgca agacaacaac ctgcccgtta tcaaacacag ccccgaattt
     3661 atcagcaata aatcgcccga tacgccgacc aaccgcatcc tgacttcgct gctttgccgc
     3721 gaacgtcttt cttttcttct gcaacacggg ctgacttacg tcaaagcaag tcaaggcttg
     3781 gtgcaaaaac acatcatgcg gtatccgcag ctatttgcca ctctcgccat tgaaaaacat
     3841 ttggcaaacg gcggcaaaaa aggcgtgatt tggcataccc aaggctcggg taaaaccgcg
     3901 cttgcctatt acaatacccg ctacctgaca cactattacg ccaaacatgg catcgtgccg
     3961 aaattctatt tcatcgtgga caggctcgat ttattgaaac aggcgcagag ggaatttacc
     4021 gcccgagact taatcgtcca taccatcgat agccgcgaag ccttcgctgc cgacattaaa
     4081 tccgcccaaa ccctgcacaa ccactcaggc aaaccggaaa tcaccgtcgt caacatccaa
     4141 aaattcaaag acgatcccga tgtcgtcgct cgcaacgact acgaccttgc catccagcgc
     4201 gtctattttc ttgatgaagt acaccgcagc tacaacccca aaggctcgtt tcttgccaac
     4261 ctcaaccagt cagacataaa cgccgtcaaa attgggctga ccggcacgcc gcttatcggt
     4321 gtaaccgcag gcaacgtcaa cactcgcgag cttttcggcg actacatcca caaatactat
     4381 tacaacgcct ccatcgccga tggctacacc ctgcgcctga ttcgcgaaga aatcggcagc
     4441 cgatacaaag cccaattaca agaagcactg gcgcaacttg aaatcgaaaa gggcagcatt
     4501 gaccgcaaag aaatctacgc ccatccgcac ttcgtccacc cgatgcttga ctacatcttg
     4561 gacgactttg ccaaattcag aaaaaccaac caagacgaca gcctcggcgc catggtcgtt
     4621 tgcgacagct cagagcaggc acgccagctt tttgagcatt ttcagacggc ctcaaaccac
     4681 aatctgaccg ccgtgctgat attgcacgat atcggcacca aagaagaacg cgaccaatgg
     4741 gttaaagact tcaaagcagg caaaatcgac atcctgtttg tgtacaacat gcttttgacc
     4801 ggctttgacg cgccgcgcct gaaaaagctc tacttaggca gactcatcaa agcccacaat
     4861 cttttgcaaa ccctgacccg cgtcaaccgc acctacaaat cataccgtta cggctatgtt
     4921 gtcgattttg ccgacatcca aagcgaattt gacaaaacca accgcgccta ttgggatgaa
     4981 ctgtccaacg aattgggcga cgaaatcggc aattacagcc agcttttcaa aaccaccgaa
     5041 gaaatcgaac aagaaattgc aggcattaaa gatgccctgt ttgaatttga tagagaaaac
     5101 gccgaagtat ttcgtcatca aatcgaacaa atagcagata aaaaacaact tcttgccctg
     5161 aaaaaagccc tgcaaaccgc cagagagctg tacaacgtcc tgcgcctgca aggcagccat
     5221 gagtttctcg agtgtctgga ttttgacaaa ctcaatcttt tatatcgtga agctgtttca
     5281 cgcttagata ccttaaacat tattgaaaaa cagcaacaag gcaatatcgc ccatttgctc
     5341 aatgaagcat tggaagacat ttacttccaa ttcgtcaaaa tcagcgaagc cgagctcaag
     5401 cttgccgacg atttgaaaga catcatgcgc aaagtgcgcg aagggctggc aggcaatatt
     5461 gaccaagacg accccgaata catcagcctg cgcgaagagc tggaacgcat cttcaagaaa
     5521 aagaacctcg ccgaagtcgg gcaggaagaa atgcaggcaa acatcgctac gctcgatact
     5581 gtctatgccc aaatcaaaga gctgaaccgc aaaaacgatc tcttgcggca caaatacggc
     5641 ggcgacacca aatacgcccg catccacaaa cgcttgctgg aaaatcccgc cctctacggc
     5701 gacaaactta aagtcttcaa cgcgctaaac ggcgtcaaaa ccgatgccga ccaaaaagtg
     5761 ctggatatgg agcagatttt ggataaccaa aattactttg aaaaacaaat gcagggcatc
     5821 gtattaaaac gctttaggac ggaacagcaa tttcccgttc agcccgccga catccaagcc
     5881 atcaaccgcc tgttggtgcg ggaatattta aaagaaagcg ggcggatttg aggaagcggt
     5941 ttttcaggca gcctttacgg gcgtattgca tacgttcgga atgggcaata aaagtgcggt
     6001 cagatttggg aatgtttttt cagacggcct ttaaagagtt tcaaaagatg aagttttaag
     6061 atgatagaaa aacggttaat tgatatttca aaaaatattt caagtggcat aacaccattg
     6121 cgaagtaatg atgaattttg gactgacgga acaatccctt ggttaaaaac agagcaactt
     6181 ggtgaaaagt atatttttga tacaaatgaa tatattacgg aaaaagcttt gcaagaagca
     6241 aatgtgaaaa tttttcctga aaatacacta tctattgcaa tgtatggaga aggcaaaact
     6301 cgaggtaacg tttcgatatt gaagcgaccg atggctacta atcaagcctg ctgcaatatc
     6361 gaactggatg aggagaaagt aagctcagaa tatgtgtatt attttttaaa gacacagtat
     6421 gaaaatttaa gagggttgtc ttcaggtatc agaaaaaacc taaatacaaa cgatattaaa
     6481 aattttgttg tcaggctgcc tgaaaacctc aaaacccaac aatccatcgc cgccgtctta
     6541 tccgctttgg acaaaaaaat cgccctgaac aaacaaatca acgctcgctt ggaagagatg
     6601 gcgaaaaccc tgtacgacta ctggtttgtg cagtttgatt tccccgacgc caacggcaaa
     6661 ccctacaaat cctcaggcgg cgaaatggta tttgacgaaa ccttgaaacg ggaaattccg
     6721 aaggggtggg aggtgaagag tttatggaaa atagcaaagt attttaacgg attagctttg
     6781 caaaaatatc gtcctgaaaa tgaactcgat gattttttgc cagttattaa aatcagagaa
     6841 atgaatgaag gtgtttcttc aaacactgaa agagctaaaa caaatatccc aaaagaagca
     6901 attattgatg atggggatat tttattttct tggtctgcga ctttagaaat caaaatttgg
     6961 tctcaaggta aaggggcttt aaatcaacat atttttaaag taacttcttc cgaatatcca
     7021 aaatactttt tttattttga attattaaac tatttgaagc attttaaaat gattgctgat
     7081 ttaagaaaaa cgacgatggg acatattacc caagaccatt tgaagcaagc gtatatctgt
     7141 attccttctc aaccattgtt agaaaaattg gaaaaaattg ttactcctat ttttcaaaaa
     7201 atattaataa ctcaaaaaca aaaccaccaa ctaacccaac tacgcgattt cctactgcct
     7261 atgctgatga acgggcaggt atctgtggcg gaataagcca gattcgagaa tcggacctac
     7321 aaaataaaac aaacgagata aaaaatgact gaacaacact ttaccgaaca aaccaaagcc
     7381 ctgattgaca gcttaaaaac catttgcgcc aactatggct taggcaatga cggcaacgag
     7441 ttcaaaatca tcagtcaggc atttttgtat aaattcttaa acgataaata cgattttgaa
     7501 gttaaaaaaa ttcgcaaaga aaatcccgac gaggaaattg agttcatcaa tatggatatt
     7561 gagggaaaaa cagccgtttt gaagcccgag cattccataa aatatctgtc cgagcggcaa
     7621 aacggtgcag attttgccaa actctttgac gatacgctga ccgacatcgc cgcgcataat
     7681 gcggaattgt tttcagtgaa aaccgaaggc ggggcgaaaa tcgtgttgtt cgagcgtatc
     7741 agtcaatata ttactgatga aggtcgccgc gacgattttt gccgcgcgct gatttccaag
     7801 ctggcgggtt ttagctttga ggtgattttt gcgcaaaaat ttgatttttt cgccacgatt
     7861 tttgaatacc tgattaaaga ctacaacagc aattcaggcg gcaaatacgc cgaatactac
     7921 acgccgcacg cagtagcgcg gattatggcg gatattttgg tgccggagga agtgcgcggg
     7981 caaattcgca gcgtggacgt ttacgacccg tcggccggtt cgggaacgct gttgatgaac
     8041 gttgcccatg ccatcggtga agataaatgt atgatttata cgcaggatat ttcgcagaaa
     8101 tcgtccaatc ttttacggct gaatctgatt ttgaacaatt tggtgcattc actgaataat
     8161 gtggttcagg gcaatacgat tttgtcgccg tatcacaaag acgcttcagg ccgtctgaaa
     8221 aaatttgatt tcattgtttc taatccgccg tttaaattgg atttcagcga tttccgcgat
     8281 cagctggaaa gcgatgaaaa ccgcgaacgc ttttttgcag gcattccgaa aatcaaggcg
     8341 aaggataagg ataaaatgga aatttatcag ctttttatcc agcatatctt gtttagcctg
     8401 aaagaaaatg gtaaagcggc gattgtattg ccgaccggct ttatcactgc ccaatcgggc
     8461 atcgataaaa aaatccgcga atatttggta gaaaacaaaa tgcttgccgg cgtggtttcc
     8521 atgccttcca atatttttgc caccacggga accaacgttt ctattctgtt tatcgataaa
     8581 gccaataaag acaacgtagt attaatcgac gcatcgggct tgggtgaaaa aatcaaagac
     8641 ggcaaaaacc aaaaaaccgt actttcccgc gaagaagaac aaaaaatctg caataccttc
     8701 acgcacaaac aggcagtgga agatttcagc gtggtggtcg gctatgatga gatcaaagcc
     8761 aagaattaca gcttgtcggc aggtcaatat tttgaagtaa aaattgatta tgtggatatt
     8821 tccgccgagg aatttgagca gaaaatggcg ggattttcgg cagatttgga caagctcttt
     8881 gcggaatctg ccgaattgga aaaagagatt aaggatagat tagcaatgtt gaaattcaat
     8941 tcataaatac tgacaaatta aactggccaa acttaaaagg ccgtctgaaa attttcagac
     9001 ggccttttgt ttcatttgtt ttgagaatca atcaagcaag attatttttc agcttcttca
     9061 aagccgacga cttcgaggcc gaagccggtg aggccggtga aggaggaagg ctggccgagg
     9121 acgcgcagtt ttttgacgtt gaggccggcg aggatttgtg cgcctatgcc gtagcttttg
     9181 ctgtcccatt tgtaggcttg gtttgcgcct ttaggcaggg tgcggtcgag cagggatgcg
     9241 ccgtcttcgg tgcggtgcag aaggatgacg acgccgcttt ctgcctgttg gacgcgctcg
     9301 agtgctttgg gcaatgacca tgaatggcgc gggttggctt gaatgaagtc catcacactg
     9361 aagggttcgt ggacgcggac gagggtttcg gtgtcggcgg agggcgtgcc tttgacgagg
     9421 gcgaggtggg tttcgccgga gagtttgtcg acgtaaacgt gttgttggaa ctcgccccac
     9481 ggggtttgta caggcgcatt gcccatgtct tcaagcaggc tttcggtacg gctgcggtat
     9541 tcgatgaggt cggcaatcgt gccgattttg agcttgtgtt cttcggcgaa tttcatcagt
     9601 tcgggcatac gcgccatcgt gccgtcgtcg ttgatgattt cgcaaataac ggaggcagga
     9661 atcagcccgt tcatttgcgc caggtcgacg ccggcttcgg tgtgtccggc gcggacgagt
     9721 acgccgcctt tttgggcgcg aagcggaaag atatgaccgg gttggacgat gtcttcgggt
     9781 ttagcggtcg gggaaacggc ggtttgaata gtcagggcgc ggtcggcggc ggaaatgccg
     9841 gtggtaatgc cgtgtgcggc ttcgatggag acggtaaagt tggtgccgta ttgcgcgccg
     9901 tttttctgag tcatcatggg caggccgagt ttttcgacca tttcgccgtc catcggcagg
     9961 cagaccaagc cgcgcgcgtg tttaatcatg aagttgatgg cttcgggcgt gacgaattgc
    10021 gccgccatca gcaggtcgcc ttcgttttct cggtcttcgg catcggtgat gatgaccatt
    10081 ttgccggctt tgatgtcggc taggatttcg gggatggggg aaatatggga catggtgtgt
    10141 tttcctgtgt gttcgggcgg gcgggcggtg tgttcggtat cgagccagaa ggcgggcatt
    10201 tttgcagcct gttcgatttt acgggctttt ttctcgccga aggatttgtt tttcaaaagg
    10261 gcggagagtt cgcctgtgtt gagggcaacg gcttcggcaa aacgggtttg caaaccgccg
    10321 tagtgttctg ctatccattg ccgcaggttg cggcggcgca gaccggctgt gtcggtcatc
    10381 gtttcatcct ttgtagaaat cggatgtagc ggattgtaag cgcgtattta ccaaaaagca
    10441 aatctgtttt ttcgctaaac cgaattattt gctttttggt aaacagatgc cgtctgaagc
    10501 cggattcgtg gcttcagacg gcattgccgc aaccccgtcg gcggggcggc tgtttcttcc
    10561 cgcccgcttc cgattcggga tttgccggaa gcttattccg aaaaaaattt ttgataaacg
    10621 gccttgattg ccgggtacag cataggcggt acgagcaggc gcaatatcgg caatcctatg
    10681 atgaatgcgg tcttcccgat aaaaaccgga cggattgcct tcgcgccgaa tatctttctg
    10741 catttttctt taaatgaaaa atatttccgg tagatgacga taatgttttt ggaaaaataa
    10801 agccttacta atatcgacct cagatagccg atattgtcga agacaaattt ttgcaaaccg
    10861 ccgtagatgg gggaattttt gttttcgact aaaaaatcca caccccggtc gagatgcgtc
    10921 agcacatcgc tgaaattttt gtatttgatg ttgtggctga tagagccgcc gagaacgcgg
    10981 tactggtaaa aagggttatc gtaaaaggca aaagtcttga taaaacgcgc caattgcaaa
    11041 ttgtacggga aatcctcgtg aatgtatcct tttgggaaaa acagattgtt tttgatgatt
    11101 atttcccgcc tgacgatttt tgtccacgcg ttggcgatat agtaccgccc ctccaccagc
    11161 gtttcaaaat ggcggacaaa atcattatcc gaaaagtccg cccctttggg gatgacgtgg
    11221 taattgaggg acgagggatg caggatcaaa tcaacctttt tgtctgcaag ttgttgtaaa
    11281 tcaaagagaa tccccccccc cgcgtttttt gaacggttgg tatcggccca atagtcgtcg
    11341 ctgtccaaaa agattaggta atcgcctttt gccgcccgga taccggcgtt gcgggcatcc
    11401 gacagcccgc cgttttcttg atgaatcact tttatatgcg gatatttgcc tgcatattcg
    11461 tcgcaaatct tcccgcagcc gtccggcgaa ccgtcatcga ccaaaatcat ttcataatcg
    11521 gtaaaatttt cggcaagcac ggaatccacg cagcagcgga ggtatttttc cacattgtaa
    11581 atagggacga tgatggagaa aatcataaat atcaatccat tatattaaga tgtttgcgcg
    11641 tatgcctcaa acccgcgctc gcaatgcgtt tgcatccact ataaaactta ttgctttatc
    11701 aaggtatgga aacctgtttc ccgaaaggcg gcgcaggatg cccgttccct gcaactttgc
    11761 cttattccga catcagatgt cccagcttgt ctgctttggt ttggaggtag cgtttgtttt
    11821 caaggttttc cccgacgtgc agggggatgc gttcgaccac gttaatcccc gcatctttca
    11881 gggtttggat tttttcgggg ttgttggtca gcagtttgac cgagcggatg cccagatatt
    11941 cgtagataga ttgcgccaaa cggaaatcgc gggcatcgac gggcagcccg agtgccaaat
    12001 tggcttcaac cgtatccata ccttggtctt gcagatgata ggcgcggatt ttgttaatca
    12061 gcccgatgcc gcgtccttcc tgacgcagat agacgatgat gccgcgcccc tctgtctgta
    12121 ccgccctcat ggccgcttca agttgaggtc cgcagtcgca ttttctcgag aacagcgcat
    12181 cgcccgtcag gcattcggag tggatgcgcg tcagaaccgg attgccgtct gaaaaattac
    12241 cgacggtcag cgcgacgtgt tcctgcccgt ttgcctcttc aaagccgtgc atcgtaaata
    12301 cgccccattc ggtcggcagg cggcaggatg ccacatggtt caacatttca gacatcttca
    12361 ctcccatctt ttccggcaga catacccaac agcagtttca aaggttcgga caaagccaag
    12421 gcaagagcca cccattccac ctgtgctgcc gtacccgcac actctgcgca ttcaaattcc
    12481 acatgaacca cgcccaacac accgccactt tccgtgcaga ccggaatgga aatttgcgcc
    12541 gccgaagcat gattgcgttc tcccgaaagc tcccccaaat ccaaccaacg gcgtacatcc
    12601 gaggcaacat tcatccaacc gctttgcgcc gaacggcaag ccagcgaaac cttgcctgcc
    12661 gcttcatcca aatcccacag gttttccaaa acctcgccct gtcgggacag gcatatcagt
    12721 tggaaagcct ggttttcaga tggcatcaag gcatagaccg ccgcactccg cacgcctgtc
    12781 gagcgcgaaa acaccgaatc caaagccatg aaaagccgtt tcagtgcatc ttcgcggaca
    12841 ttgtcgcacg acaggtaatc ggcaagtttc caatcctcat cactgcgcca caaaaccgaa
    12901 cggtctatcg aagccgtccc catatccatt accgtctgcg ccgtcagata cgccgcccga
    12961 acctcgtcaa gcggcagctt caaaccctga gtcagcagaa aatccttaat caaagcagca
    13021 ggcataccca aattccgtca ataaaaacaa aaaaccgccg gattccgatg tcaggcggac
    13081 gaaaaacaga ttttaccgcc ccaaacccca agccatcaac agccccgccg aaaaccttgc
    13141 acaaaaaaac aaaatctgta taatcgtcca attcccatgc ggacgtggcg aaattggtag
    13201 acgcaccaga tttaggttct ggcgccgaaa ggtgtgagag ttcgagtctc tccgtccgca
    13261 ccaataatga taaataaatc atatagttaa tgcttatcgg gacaaattag tgccacaaaa
    13321 cataagtcgt ctgcttttca gcgggcggct tttttatctg cctatatgtt tcaacacgca
    13381 gaacggtaca taaagtgccg ccatttgttc taccccgaat cagaagcggg tgcttccgcc
    13441 cgcaatcaga ttcaccgccc ctaatgccgg ggtttcaact ggaaagaaga aaatattgat
    13501 tttgatgatg gcatcaaaaa accccttccg cccgtctgcc aaaaggaatg gttaagaaaa
    13561 gcaatgcgtc ttttgatgga acgcgatata taaggaattg aggggaaggg caggcgggtt
    13621 tctgcccgtt aatcctgcgg acgatcccgc caaacccgac acgcgcgggc atatggcgga
    13681 ttgtttacaa tattgtctaa aaatctgaac ttgcaaacgc gaaataaggt agaatacgcc
    13741 ccgttatttt accgtccaat ccccatttac caaggaaaaa tgatgagtat ttcattgagt
    13801 taccgcgatg caggtgtcga tatcgacgca ggcgaccaac tggtcgaaaa catcaaacct
    13861 tttgccaaac gcacgatgcg cccggaagta ttgggggatt tgggcggctt cggcgcattg
    13921 gtcgaaatcg gcaagaaata taaaaatccc gtattggtca gcggtacgga cggcgtgggt
    13981 accaagctca aacttgcctt tgattgggat aaacacgata cggtgggcat cgaccttgtt
    14041 gcaatgagtg tcaacgatat tttggttcaa ggagcagaac ctctgttttt cttggattat
    14101 ttcgcctgcg gcaaattgga tgttccgcgc gcaaccgatg tcatcaaagg cattgcacaa
    14161 ggttgcgaag aatccggctg cgccctgatt ggcggggaaa ctgccgaaat gccgggcatg
    14221 tatcccgtcg gagaatacga tttggcgggt tttgccgtcg gcgtagtgga aaaagagaat
    14281 gtcattaccg gccgcagcat cggcgcgggc gatgtggtat tgggtttggc ttccaacggc
    14341 gcacattcaa acggctattc ccttatccgt aaaatcatcg aacgcgacaa tcccgatttg
    14401 gatgccgagt ttgataatgg caaaaccttg cgtgaggctg ttattgcgcc gacccgtctg
    14461 tatgtgaaac cgattcttgc cgctttggaa aaatttacca ttaaaggtat ggcacacatt
    14521 accggcggcg gcattaccga aaacgtgccg cgcgtgttgc ctgaaaacac ggttgcacaa
    14581 atcgatgcta aatcgtggga attgcccaag ctcttccaat ggcttcaaaa ggcgggcaat
    14641 gtggaaaccc aagaaatgta ccgaaccttt aactgcggca tcggcatggt cgttattgtt
    14701 gctgccgaag atgccgatgc ggttcagggt ctcttgggtg aacaaggtga aacagtttac
    14761 cgtttaggtt tgatccgtga gcgtcaggga gacgagcatc aaacccaggt tgcctgattg
    14821 cttctatagc gaaatgccgt ctcaaacatg ggtttggacg gcatttttat agagga
//
LOCUS       DAWFBO010000036        14772 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFBO010000036 DAWFBO010000000
VERSION     DAWFBO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.036"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(24..719)
                     /locus_tag="WGO87_000791"
     CDS             complement(24..719)
                     /locus_tag="WGO87_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0392173.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(890..1549)
                     /locus_tag="WGO87_000792"
     CDS             complement(890..1549)
                     /locus_tag="WGO87_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0392174.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1747..3858)
                     /locus_tag="WGO87_000793"
     CDS             complement(1747..3858)
                     /locus_tag="WGO87_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0392175.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GNSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSIDTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDPKNNPYIYAVSGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYDSRNKEVTTLPGFARVDAMLGWNHKNVNVTFAAANLFNQKY
                     WRSDSMPGNPRGYTARVNYRF"
     gene            complement(4044..4649)
                     /locus_tag="WGO87_000794"
     CDS             complement(4044..4649)
                     /locus_tag="WGO87_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0392176.1"
                     /translation="MTRQSLQQAAESRRSVYLLNKNLPIGKDEIVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLKLFKAGAATILFYED
                     QNVVKGLQEQFPAYASNFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4699..5097)
                     /locus_tag="WGO87_000795"
     CDS             complement(4699..5097)
                     /locus_tag="WGO87_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0392177.1"
                     /translation="MSNCCNRIQPVLLSVLRIVTAYLFLLHGTSKFFAFPIEMGSGSP
                     GGLMLLAGILEVVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFFYIAAAGGGAWSLDRLFFKRKA"
     gene            5205..6116
                     /locus_tag="WGO87_000796"
     CDS             5205..6116
                     /locus_tag="WGO87_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0392178.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLIRNGVPKTPEEVTRLPAVLPTYTNQQKLDITHKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAVEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6106..7407
                     /locus_tag="WGO87_000797"
     CDS             6106..7407
                     /locus_tag="WGO87_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0392179.1"
                     /translation="MPSETARPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPNLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDSPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(7475..7699)
                     /locus_tag="WGO87_000798"
     CDS             complement(7475..7699)
                     /locus_tag="WGO87_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392180.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVAAWVAVIWAYLMIESEKNGRY"
     gene            7802..9952
                     /gene="dinG"
                     /locus_tag="WGO87_000799"
     CDS             7802..9952
                     /gene="dinG"
                     /locus_tag="WGO87_000799"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0392181.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            9988..10602
                     /locus_tag="WGO87_000800"
     CDS             9988..10602
                     /locus_tag="WGO87_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0392182.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGAPVAGAASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRASVTQSDFQPAGETEETYSRTILRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAGWLAAEYRLPASIEDIAASVGNDEILAA
                     EAYLAARLVCADLSRKETVFLARLSQALELDDNLVENLERQLGF"
     gene            complement(10707..12575)
                     /locus_tag="WGO87_000801"
     CDS             complement(10707..12575)
                     /locus_tag="WGO87_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0392183.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILAEIRDGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSANENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTASSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTENGKQQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFISFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLVEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRIGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSHGGALQ"
     gene            complement(12655..14025)
                     /gene="purB"
                     /locus_tag="WGO87_000802"
     CDS             complement(12655..14025)
                     /gene="purB"
                     /locus_tag="WGO87_000802"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0392184.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAASEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTDMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANIVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPVSRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKVFIESLEIPTEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(14097..14729)
                     /locus_tag="WGO87_000803"
     CDS             complement(14097..14729)
                     /locus_tag="WGO87_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0392185.1"
                     /translation="MENVNRVPEQARYDAERRQADEVLAGVFPAVSIFGSARTPQNHA
                     DYTFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYIVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         3527 a         4105 c         3804 g         3336 t
ORIGIN      
        1 cctttactca ttcaatcctc aacttattgc ggctttttct gctcttcgcg tactttatcc
       61 accaactggt caatcgtggt cataccggac tgccagtctt taaattcaac ttggtatttg
      121 ccgccgacaa tcacagtcgg tgtgccgctg atttggaatt tattggtcaa ctcttccatt
      181 tgagccgcac gcgcttggct ttcaggagcc tcaaatgcag ccaatacttt tttgccgtca
      241 aacgctgttt gctcggacag ccattttttc agggtatcgg tatcggccag attgattttt
      301 tgattaacca tcgcatcgaa aatatggctg ttggctttat ctgattcacc ggccatttcc
      361 actgcggccg ccaaacgtgc caaaggtttc atttcatcac cccacacgac atgctcccgg
      421 cgcatatagg tatcgtcttt aaacgttttg atgtgctcgc tcaagaccgg ctcaagatgg
      481 gcgcaatgcg ggcagaagta gccgaaaaat tccaatactt cgattttacc ggcctgctgt
      541 tgcggaatag gcgtagacaa tacagtgtag ttcacacctt cgttcaactc agcaggggct
      601 gccggagcag atgagctgct ttggacgctg tctgccggaa cgctggtttc agcctgtttg
      661 ctacaagcgg ccaatgccaa cagggtcaat gaagtcaaag ctaaggtttt cagtttcata
      721 ggtatctctt gtgtgtcaga ttaatgtgcg gattttatga cattttattg aaggcgtgtt
      781 cgatttgagt gaaaaaatgt gtttagtttt atttcagcct ttataaaggc cgtctgaaat
      841 atggcttaga taattagcct tattttcaga cggcctttgt tctagtaatt tacggtttcg
      901 gacattttgt cttcaatccg tcgatgtgtt ccattaagtc aggtgggaca tgattccaca
      961 taggatcaag aataaaatcg ataccttccc gtctggctaa tttggctgct gggacaaaat
     1021 cggaatcacc agaaaacaag atgattttat cggcttgttt ttttaaggta atagatgaaa
     1081 tatccaagcc tatacgcata tctacacctt tttgtttaac atcgagcacg acatcgtttt
     1141 ccgtaatttg agttgacggg tttttcagaa gatttttcat gacttggggt tttaatatcc
     1201 atgctgtatt gtccgacagc ttgcctaaac gtaacgctaa tttacgcttc ttcttcaatt
     1261 cggtatgcag attgcaacga aatatggctt ccggagtctt tgaaaaatct atggcacgat
     1321 gagatacggg attgtgcatt ttcttttcaa acggcacgca gtcgtaaaag aaaattctat
     1381 ataaatcatc catttgatct gtgccgttga ttttatcttt cagatgcagc atcgaatatc
     1441 ggaaagccaa atcagcaatt ctttttgcat cgtgtggatt ttgagacagg cctaatgacc
     1501 gtacccgctt aataaaaaat gcaccgtcaa tcaaaatggc ggttttcata ttgcttcccc
     1561 tatatttgtc aaagatataa aaaagccctt gggatcggca tacccgctat gatttaatgg
     1621 gtaatgcgta ctgccaaggg cactatttct ttttggatta tactgatgta gataaagcct
     1681 gtcaaaaagt tcgtatgctg aatttacgga ttatcaggat tttcagacgg cctttgcctg
     1741 atttcatcag aaacggtaat ttacccgggc agtatagccg cgcggattac ccggcataga
     1801 gtccgaacgc caatattttt gattgaacag attggctgcg gcaaaggtaa cgttaacatt
     1861 tttatggttc cagccaagca tggcatcaac tcgggcaaag cctggaagcg tagtcacttc
     1921 tttatttctt gagtcgtaac cgtagcgttt gcctgtaccg gttacgccga tttcgccgta
     1981 gaggttttcg gtcggtgtat aacggaaaaa caggttgccg gtaacgttgc tggtattatt
     2041 caaatggatg cccactcggt cgggattttc tttatcttca acgactttcg cctgcatcac
     2101 gcccaacgaa ccgcgcagat agagtttttt agggatgatt tgcccgatgg cggacaattc
     2161 cacgccgcgc gaacggtgtt tgccgctaac cgcataaata taagggttgt tttttggatc
     2221 ggggcggtag cggatattga agcgttcgat ttggtaggca gacaacgtag tgctgaggcg
     2281 gtcgtccagc caactgcttt tcacaccggt ttcgtattgg cgggtgtact cggggtcggc
     2341 gttgaacacg gcggaagacg acgtatcgat gctcaaatag ccgccgcgtc cgccataagg
     2401 cgcgaagcct ttgttatacg aggcgtaaag tgtgtggact gggttgatgt tccacactgc
     2461 gccgatgttg gggctgaacg agtgtccgct gtattggcgg ctgttgccgg tgagtttgtt
     2521 ttcggaatta aaggtgtatt tgtcgtaacg accgccgagg acgaatttca agtcgggcgt
     2581 ggcggagaag atgttttgca caaagatgcc gtaggagtcg gctttgtggc ggttttgggt
     2641 gaggataggc tgcaatctgc ccgaagccgg ccagcttgct cggtcgtatg gatcgatgga
     2701 agcagtaaag gcgcggctgt aacctaaggt cgggttgcgg tgttcgcggc tgtaatccat
     2761 gcctacggtc aggtggtttt caaaacggcc gatggtgtag tcgccgttta gcgtgaaatt
     2821 ggacgacagg gttttgttgt cggtctgctg ccaggcgtag ttacgtttga ttaagttgcc
     2881 attttcgctg cctgcataga aatgatcaaa atcctgcgcc gccgtgcggt gggcgagctg
     2941 ccattgggca cgccatttgt cgttgaaggc gtattcaagg tcggaacgcc aaacttgcag
     3001 cttgtctttg acaaaatcgt tccggtgggc gaaccccatg cggtaaggca gtccgaagcg
     3061 gtcgtacacg gacttggtcg gactgcggtc gggcgtgcgc tccacattgt cgtaggtgta
     3121 ttgccccgtc cacttcaagc cgttgtcgag tttgacggta atgctgggcg aaaccatgac
     3181 atttttgctg tctatgccgc tgcggaacga attggcgcgc ccgacttcgc cggtgagacg
     3241 gatggcgacg tttttgttca gcacttcgtt aatgtccata ttcaggctgc ggtttgccca
     3301 cgaaccgtaa accgcaccga tgttgcggct ttgtttgaag ttggcgtatt tgctgaccat
     3361 gttgatgacg ccgccgccgt tggtgcggcc gtaaagcacg gaagacgggc ctttcaggat
     3421 ttccacgcgc tcgatgttgg cagtgctgcg gcggacctgc ccgctttcgc gcacgccgtc
     3481 acggtaaata tcggatgcgt cagcttgaaa gccgcgcagg aaaatgcttt cgccgcgcat
     3541 atcgtaggca gcgtcgatgc cggcattgcc ttcgaggatg gaactcaaat cgttcgtacc
     3601 gtaatttttg tttttctgga tattgagcgt atcgatggtt tgcggcgttt ctttgatgag
     3661 ctgtccgttg cgggtaacgg cggcttcgtc gtagttgatg tagcctttga gtacgctggt
     3721 gtcggactgt ccgaccacgg aaacggtagg tagcgtggcg gtgtaatgtt caccattgtc
     3781 ctgcgtatcg gcggcagcaa cagggaagga agcaataatc agcgtgggta ataaagctaa
     3841 atgaaatgat attttcattt ttatactcaa tttaacaaaa caaccgaatt atattgcctc
     3901 acggaggaaa tgagaataat ttcttttaac tatattgaac atgatttttg taaacaaagg
     3961 tctcagaatg cggaaaactc gccgcctgat actgaaaaat gccgtctgaa cagggttcag
     4021 acggcatttt tttgaccgcg aaattatgcg ccgaacactt tcaaacgttc tgcaactggt
     4081 tcaaaggtct tttcacctgc cgccccttca ataccgccaa taaccatttg cgcgcgcaac
     4141 aaccagtttt cggggatatt ccacgctttg gcaatcgccg catcgggcaa gggattgtaa
     4201 tgttgcaggt ttgcacctac gccgaccgcg gcaagtgtcg tccaaacggc atactgcacc
     4261 atcgcgttcg cctggtccgc ccaaacggga aagttggagg cataagcagg gaactgctcc
     4321 tgcaaacctt tgacaacatt ttgatcttca taaaacaaaa tagttgccgc acccgcctta
     4381 aacagcttca atttttgcgc ggtcggttca aaactgtcgg caggcacgac ggcacgcagc
     4441 gcgtcttcga caaattgcca caccttatca tgctcttcgc caaacagcac gaccacgcgg
     4501 gcagattggg aattaaacga agaaggcgtg tgcaaaacgg cgtgttcgac gatttggacg
     4561 atttcatctt tgccgatggg cagattttta tttagcaaat aaacggaacg gcggctttcc
     4621 gcagcctgtt gcagggattg acgggtcatt tcttttcctt tcaaaaatat tcgcaaaata
     4681 cgcttcagac ggcatagttc aggctttacg cttgaaaaac agcctgtcca gcgaccacgc
     4741 gccgccgccc gccgccgcga tatagaagaa tacgaagcag aacagcaccg cggactcgcc
     4801 gccgttggca atcggaaaca aagcatttcc ggaagcgtgc gccataaaat aggcaaccgc
     4861 catctggccg gacaaaacaa acgcggcagg gcgcgcaaac aggcccaaca ccagcaaaat
     4921 gccgccgacg acttccaaaa taccggcaag cagcatcagc ccgccgggcg aaccactgcc
     4981 catttcaatg gggaaggcga agaattttga cgtaccgtgc aacaaaaata ggtaggcggt
     5041 tacgatacgc aaaacagaaa gcagaacagg ttggatacgg ttgcagcaat tggacataaa
     5101 agctccttcg gtatttatct tcatttcagg cggcagagta tagatgtttc cccttttcaa
     5161 atatatactg cttttattga tacaactttt ccacacaaga aaatatggac accctgttca
     5221 gcctcaaagt tttccgccaa gtcgtccaaa gcggcggctt tacccgcgcc gccgacgcgc
     5281 tcggcatctc caccgcaatg gcaagcaaac acgtcagcca cttggaaaac accgtccaag
     5341 ccaaactcct gcaccgcaac agccgcaatc tcagcctgac cgaagcgggg gaagaatact
     5401 accgacaatg cagttacgcg ctcgacacgc tcgacgatgc cgcgcaaaaa gccgcagggg
     5461 ggacggaaaa accgcagggg ctgctgcgcg taacgatgcc gctgtggttt gccggcagcc
     5521 agatatgcaa ctggctggcg gaataccgcg aacgttatcc cgaagtaaca ttagaactga
     5581 ttttggacaa ccggcacgtc gatttgattg ccgaaggcgt ggatttggcg ttgcgcgttt
     5641 cccaaaccct gtccccgtcg ctgattgcgc gcccgctggc ggaaatcgaa tttgccctgc
     5701 tcgcctcgcc cgacttcctg atacgcaacg gcgtaccgaa aacgccggaa gaagtaacga
     5761 ggctgcccgc cgtcctgccg acctacacca atcagcagaa actcgacatc acccacaaat
     5821 cggacggcaa aaaataccgg cttgagctga cccccgtcat ccgtaccgac aacacgctga
     5881 tgatgcgcga aatgattaag gcgggcgcgt gcatcggtta tcagccgctt tgggcggtgg
     5941 aacacgattt gcgctgcggc acgctggtga ggctgctgcc cggatacgcc gtcccgaccg
     6001 accggctgaa tgccgtttat gcagacaggg cattcttaag cgcgaaagtc cgcagcttca
     6061 tcgattttct gaacgaaaaa atcgccagca ggaaaggctg ccgaaatgcc gtctgaaacc
     6121 gcccgccccc tttatgcgga cacgcgcgcc gcacacacgc tcgtctggtt ccgccaaaac
     6181 ctccgcatcc gcgacaacgc cgccttatgc gccgccgttg ccgaaggttt gcccgttatc
     6241 ggtatttgga ttgacgatgc cgaaacagac aaccctcgcc gcgccgcgtt ctaccgccaa
     6301 tccgccgccg aactcgccca agggcttgca gggcgcggca tcccgctcta cacggcggca
     6361 tcgcctgccg agctcgtccg gctcgccgtc cgcctcaata tccgcgccgt catcgccgac
     6421 gaatcccata cttttgccga caaactcgcc gacaacgccc tttggcatga attggacaaa
     6481 cacggcatcg cgttaacctt cgtcaatgac cgcgccgttt tcggcaaaac cgacctgata
     6541 cccgacggcg gcacggcaca caccgatttc gaccgctacc gcgaagtatg gctcgaccgc
     6601 ttttccaagc agccccccgc cggtccgaac ctattcgcgg cataccgcca acccttcccc
     6661 gaaaaccttt ccgccccgcc gcctgccgcc ctttcagacg gcatcttcct gccgcaaaac
     6721 agcggcgaaa cggcggcttg gcggcagtgg cggcggtttc tcgaacaggc ggattcctac
     6781 tccgttttaa aggatttccc ctcgcgcaaa cacacttcgc tgatgggcgc gtatttgagt
     6841 gccggctgca tctcgccgcg cctgctcgcg cgggaaagcc tcgaacgccg tctgaacgcg
     6901 tgggcggaca acatcatccg ccgcgatttt ttccttcaac ttgccttgca gcacacggat
     6961 gacagccctt cagacggcaa tccggaacac accctgcgcc tgacgctttg gcagcagggc
     7021 cagaccggca ttccgattat cgatgccgcg atgcgctgtt tgcacaaaac cggcagcctc
     7081 caccccgccc tgagacgctt gagcgcggat tttttctgcc acgttttaaa cctcccccgc
     7141 cgcgaaggcg agatatggtt tgcccggcag ctgaccgatt tcgatgcggc aatcaaccaa
     7201 ggcaactggt ggcttgccgc ctcacggcac acctgccccg acattgccgc cgcctcatac
     7261 cgtaccgacc cggacggtac ttttatcaaa cggcacattc ccgaactcgc ccacctgtcc
     7321 gccgacaccg tccacacgcc ttggcggttt gcctgttcgg tcgataccca cggctatccc
     7381 gcctatcctg tcgccggtat tccctaagcg gtacggcaac ctaaaacaaa tgccgtctga
     7441 acttccgttt cagacggcat tgcgtccgaa tgactcaata tcttccgttt ttttcacttt
     7501 caatcatcag gtatgcccaa atcaccgcca cccaagccgc caccgttccc atcgccggca
     7561 aaaaatgctg ccaatccgtc atcggcggca cagggcaacg gccgcattcg atttccgcgc
     7621 cgcccaacaa ccggtacagc aacatatccg caatccaaag caacaacggg tatttcaaac
     7681 gctgcaacca aaaaaccaac cacgtccgca cgattcacct caccccccat tccgacaacg
     7741 atattgtacc gcttccgtgc aatgttaaaa taagcccact tctaccgccg tacaaaacgc
     7801 catgctcacc gatttagaaa aaaacgccat ccgcgaccat taccaaaaca tcggcaaaaa
     7861 cctgcccggt ttccgtccgc gcgcttcgca gcgggaaatg attgcggcgg ttgccaacgc
     7921 tttttcgcgg acgttggcgc gcgaagaagg cggcgagccg cccaagcgcg agggcgagag
     7981 catcgccgta atcgaagggc cgaccggcgt gggcaaatcg cttgcctacc tgctggcggg
     8041 cggcatcatg gcgcagacgc gcggcaagcg gctgattgtg agcagcgcaa cggttgcctt
     8101 gcaggagcag ttggtagacc gcgacctgcc gtttctggtc gaaaaaagcg gtttggaact
     8161 gaccttcgca cttgccaaag ggcgcggccg ctatctctgc ccctacaaac tctatcaact
     8221 gacgcaaagc aatgcccagc aaaacctgct cggctttgaa gcccccgccg tcttgtggga
     8281 cagcaaaccc aagcccgaag aattgaagct gctgcgcgac atcgccgacg aattttccgc
     8341 ccgacggttc aacggcgacc gcgacacttg gccggaaaaa atcgatgacg cgatttggct
     8401 caaagtgacc aacgaccgcc acggctgcct gaaaaccgcc tgtcccaacc gtccggaatg
     8461 tccgttttac ctagcacgcg atgtcttgga aaccgtcgat gtcgtcgttg ccaaccacga
     8521 tcttctgctt gccgacatca gtatgggcgg cggcgtgatt ctgcctgcgc ccgaaaacag
     8581 tttctattgc atagacgaag cgcaccacct gcccaaaaaa gccctcagcc gttttgccgc
     8641 cgaacattca tggaatattg ccgtttggac gctggaaaaa ctgccgcagc tgaccggcaa
     8701 aattgccgcg cttaccgata aagccgaact tgccaacctg gccgacgaag ccgccgcatc
     8761 cttgctcgac agcctgcacg aatggcagtt ccatttggcg gaagagccgt ctttaagtat
     8821 ggggccgtct gaaaacgaca gacgaaccaa cagcgaaccg acttggctgt gggaagacgg
     8881 caaaatcccc gaaggcctcg aaaccaccgt ttccaatacg gccattgctg cgcgcagcct
     8941 gctcaaacac gttgtcgggc tgaacgatgc gctttccgcc gcgcgccgcg aaaaagaaca
     9001 ggacggcgcg ctcctcgacc gcctgaccgg cgagttcggc atcttcatcg tccgtatcga
     9061 acaaatcagc gcggtttggg atttgctctc caccgtcccc ctcgaaggcg aagagccgct
     9121 ggcaaaatgg ataacccgcc gtgccgacga caaaaacgac tacattttca acgccagccc
     9181 catcagcagc gcatcccacc ttgccaacag cctgtggcgg cgtgcggcag gcgcggtatt
     9241 gacttccgcc accctgcaat ccttgggcaa cttcaacctg atgctgcgcc aaaccgggct
     9301 gcaatggctg cccgaaacca ccaccctcgc cctcaaaagc ccctttgact ttgaaaaaca
     9361 gggcgaactc tacatccccc ccatatacgc cagccccaaa gaccccgaag cccacaccgc
     9421 cgccgtcatc gaatggctgc ccaagcttat ttcgcccacc gaagccatcg gcacgctcgt
     9481 cttgttttcc tcgcgcaaac aaatgcagga tgtcgccctg cgcctgcccg gagactacct
     9541 gccgctcttg ctcgtacaag gcgaattacc caaagccgtc ctcctgcaaa aacaccaccg
     9601 ggccatagaa gaaggcaaag ccagcatcat cttcggactc gacagtttcg ccgaaggact
     9661 cgacctgccc ggcaccgcct gcgtgcaagt catcatcgcc aagctgccgt ttgcgatgcc
     9721 tgacaacccc atcgaaaaaa cccaaaaccg ctggatagaa cagcgcggcg gcaatccttt
     9781 tattgaaatc accgtccccg aagccggcat caaactcatc caggccgtcg gccgcctcat
     9841 ccgcaccgaa caagactacg gccgcgtaac catcctcgac aaccgcatca aaacacagcg
     9901 gtacgggcag caattattgg ccggtctgcc gccgtttaaa aggatagggt aaacctaccg
     9961 cccttcccgc aacagaaagg aaaagtcatg aacttcaccc gactgctcaa ccaagtctta
    10021 agcacggttc aaaaaaaagg caacacattc tccgacagcc cgctcaattc attcggcgga
    10081 ggcgcgccgg ttgccggtgc cgcctccatg ctgctgaacg gtaaaaaccg caaaaccatt
    10141 accaaaatcg gttcgaccgc cgctttgggc tacctcgcct accggggcta tcagatgtgg
    10201 cagcaaaaca aagggcgggc atccgttaca caaagcgatt tccaacctgc cggagaaact
    10261 gaagaaacat acagccgcac catattgcgc accatgatag ccgccgccgc ttcagacggc
    10321 atgatagacg aagccgaacg ccggactatc gaacaggaaa gcggcacaga ccccgaaacc
    10381 gccggatggc tcgccgccga ataccgcctg cccgcaagca tcgaagacat cgccgcctcc
    10441 gtcggcaacg atgagatact ggcggcggaa gcctatctgg cggcaagact ggtatgtgcc
    10501 gatttgtcgc ggaaagaaac cgtcttcctc gcccgcctgt cgcaggcttt ggaactggat
    10561 gacaatctgg tggaaaactt ggaaaggcaa ttggggtttt gattcaaaat tccctcccgc
    10621 aggggaggga aaataaaaac cgccgccgcc acgaagggca aacggcggca taggcagcta
    10681 caggcaatat ccggcaacaa ggcgggctac tgcaatgcgc cgccgtggga agatttaagc
    10741 aggcgttcgt gtttgtggaa ttcccgcccc atataataag aaccgaaata ggtcaggtgg
    10801 tcttggtcgc cgtaaagata gcgtccgtgt atttcgaccg tgtttttggg caggtatttt
    10861 tgtgcgtcca cccaatgcac attgggaata tctttaatca aatcaaagac cgcctgattg
    10921 ctcttgccga tgtcgcccat agcctgaatg gggcggagat attggtttgc ggcaaatctt
    10981 ttcaattttt cctccctcag gggcgaacgg ctgattgatg tgttgtttgc aaaaacatag
    11041 acgggtttga cggcggctat ccttttgacg gtttccctga atcgggcttg gaacccgggt
    11101 attaggaagg attgcgcttc aaatctcggc acgggctggc cgcctatcct caaatcatag
    11161 aattgggcaa tgaaaacggc ttcggctttt tcaacttcat cccggtattt tcgacataac
    11221 gggttgtctg ccagcttctc atctacccaa accaaacact ccgaatcgag ggacaggatt
    11281 ttggctttcc acccttcccg gctgccgaca taatccagaa acccccgcag gtgtccggcg
    11341 tgcgagtcgc cgagggtcag gacggtttcc ggaaaatgat tttccgcagc aaggggcgcg
    11401 ccgggcaacg ggcggaggtg ttcctgtttc aatatccccc ttgcgtacag gttgtaaccg
    11461 acaagtatca gggacggggc gagatagagg cagaaaaatg cctttttgaa ggtcatcttc
    11521 cgttttctaa gcggctgttc aaccaaataa taactcaaca gggaaaatcc ggccgtcaat
    11581 gcggcaaccg ccgatacggc aggcagtccg agctgtttgt cgcctgtaat gtaatgggcg
    11641 aaggaaataa aaatccaatg gtacaggtat agggaataag agattttgcc gacaaatacg
    11701 atggggcttg ccgacaggat gcgtgtcgga agtgtcccgt attgcatact ccggataagc
    11761 agcgccgtca gcaggcaggg aaggagcagg gtcattcccg ggataaacgg attgtgtttg
    11821 tcaatcacga acaggcaggc aagcaatgcg ccgaagcaga gtgatgaaag caactgccgt
    11881 tttccatttg ctgtttgctg tttaccgttt tccgtttgcc cataaaccgc cagcagcgaa
    11941 cctgccaaca gctcgggaaa cctcagtgtc gaaaggtaat aagtattggg ttggttgagg
    12001 atgtcggtat aaaacccgct tggcaaaaac gatgaggcag tcaaaatcag aaacaggatg
    12061 atgctgatgt tacgcagcac ccgtagcgat ttggtttttt tgcagcagaa tatcagcaaa
    12121 aggggataca ggaggtaata ctgttcctct accgccaaag accaaatatg cagtacgggg
    12181 ttctcgttgg cactcaaatc gaaatacccc tgctgaaacc ccaaataaat attggacaag
    12241 aaaaccgcag aaagctccac ggttttccgc atttggttga aatcttcgta aaggaagatt
    12301 tgagaggcaa tcaccgaagc cagcgacacg gccgcaataa aggcaggata aatccgctta
    12361 atcctgcggg tataaaaatc ccggaaagaa aaagaaccgt cccgtatttc ggcaagaatg
    12421 atgccggtaa tgaggaatcc cgagatgaca aagaaaatgt ccacccccag gaatcctccg
    12481 ggcagccaac ggttattcag gtggaaaata atgacggata gcacggcgac agcccgcaat
    12541 ccgtcaattt ccggtctgta tcggacagct tgcataaata tcgcccctgt atatccggta
    12601 ttttaaaaat gccgtctgaa cgcgcgttca gacggcatcg gtttcagtaa acgctcaaat
    12661 ccgtttcgcc aacgcttcag ccttgcccac atacagtgcg ggggtcagct caagcaattt
    12721 tgctttggct tcggtaggga tttccaacga ttcaatgaag actttcagca cttcgggcgt
    12781 aatgccgcct ttgccgcgcg tcaggtcttt cagcttctcg taaggattgg cgacaccgta
    12841 acggcgcatc acggtttgaa tcggctcggc aagcagctcc caagtggcat ccaaatcggc
    12901 ggccagcgcg gcggggttgg gttcgagctt gttcagaccg cgcaaatggg cggcgaaacc
    12961 caacacggca tagcccacgc ctacgcccat attgcgcaat acggtgctgt cggtcaaatc
    13021 gcgctgccag cgtgagacgg gcagtttttc cgccaaaaaa cccaatacgg cgtttgccat
    13081 accgaggttg ccttcagagt tttcaaaatc gatggggttg actttgtgcg gcatcgtgga
    13141 agaaccgact tcgcccgctt tgactttttg tttgaagtaa cccaaagaaa tataacccca
    13201 aacatcacgg ttgaagtcga tgagaatcgt gttgatgcgg ctgagtgttt ggaaaaactc
    13261 cgccatatag tcgtgcggtt cgatttggat ggtatagggg ttgaaggtca gaccgaggct
    13321 gatttcgaca aagttgcggc agtgtgtttc ccaatccaca tcgggatagg ccgccatatg
    13381 ggcgttgtag ttgccgaccg cgccgttgat tttgccgagg aactcttgcg cctgaaggat
    13441 tttaaactgg cgttgcaggc ggtacacgat attggcggtt tctttgccca aagtggtcgg
    13501 cgtggcgggc tgcccgtggg tgcggctcat catcgggacg gcggcaaggt cgtgcgccat
    13561 atcggtgagt tttccgatga tttcggcaag tttgggcagg atgacggttt cgcgcgcttc
    13621 ctgcagcatc agggcgtggg acaggttgtt gatgtcttcg ctggtgcagg cgaagtggat
    13681 gaactcgctg gcagcagcga cttcgggcac ttcggcaaaa cgttttttca accaatattc
    13741 gatggctttg acatcgtgat tggtggtcgc ttcgatggct ttgacggcgg ccgcgtcttc
    13801 aagtgagaag ttttcaatca ccgtgtcgat ttcggcaagc gtttcggcac tgaagggcgg
    13861 cacttcgaca atcttcggct cggcggcgag ggctttgagc cagtttaatt cgactttgac
    13921 gcgcgccttc atcagaccgt actctgaaaa aatcgggcgc aatgcttcaa cggattgggc
    13981 ataacggccg tctagggggg aaagcgaggc gatggggttg atcatttcgg catcctgttc
    14041 ggaaaagaca tcaaaaattg ttaaattgtt tgcaattata catcactctc aggacgctat
    14101 gccgtctgaa gcccgtgttc cgacagatac gcaacgattt cgtcttcatc gtccgatatg
    14161 gcgaacaaag aagcggcccc ttcggaaatc aggccgcgcg ccaaaagctg cgcgtttatc
    14221 cactccgcca agcccgacca aaacgccttt ccgaccaaaa cgaccggacg cggcggcact
    14281 ttgcccgtct gcaccaaggt caggatttca aacaattcgt ccagcgtccc gaagccgccc
    14341 ggcatcacga tatatgcttg ggaatagcgg aaaaacaccg ccttgcgttc ggcaaaacgg
    14401 gaaaaccgca aggcgatgtc ctgatacgga ttcggtttct gctcgtgcgg caaaacgatg
    14461 ttcagcccca ccgaaaccga cttcccggca aacgcgccct tgttcgccgc ctccataatc
    14521 cccggcccgc cgcccgaaat aacggcaata cccgaatccg acagccgcct cgccagacgg
    14581 caggcaaacg tataatccgc atgattctgc ggcgtgcgcg cgctgccgaa gatactgact
    14641 gccggaaaca cgcccgccaa tacttcgtct gcctgccggc gttcggcatc gtaacgcgcc
    14701 tgctccggca cgcggtttac attctccatt ccgtcctccg tttaaaaacg gcgattgtac
    14761 cccgtcaaaa ac
//
LOCUS       DAWFBO010000037        14063 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFBO010000037 DAWFBO010000000
VERSION     DAWFBO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14063
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.037"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(69..2045)
                     /locus_tag="WGO87_000804"
     CDS             complement(69..2045)
                     /locus_tag="WGO87_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0392186.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKVLVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIVRVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPIYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGETGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WGO87_000805"
     CDS             2194..3033
                     /locus_tag="WGO87_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0392187.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARTRYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3232..4263
                     /gene="gap"
                     /locus_tag="WGO87_000806"
     CDS             3232..4263
                     /gene="gap"
                     /locus_tag="WGO87_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0392188.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FASKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGKLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4415..5140)
                     /locus_tag="WGO87_000807"
     CDS             complement(4415..5140)
                     /locus_tag="WGO87_000807"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0392189.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(5210..5644)
                     /gene="fur"
                     /locus_tag="WGO87_000808"
     CDS             complement(5210..5644)
                     /gene="fur"
                     /locus_tag="WGO87_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0392190.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHSEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGGHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5863..6240
                     /locus_tag="WGO87_000809"
     CDS             5863..6240
                     /locus_tag="WGO87_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0392191.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            6250..7059
                     /gene="dapB"
                     /locus_tag="WGO87_000810"
     CDS             6250..7059
                     /gene="dapB"
                     /locus_tag="WGO87_000810"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0392192.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7056..7301)
                     /locus_tag="WGO87_000811"
     CDS             complement(7056..7301)
                     /locus_tag="WGO87_000811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392193.1"
                     /translation="MPRKQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPFQIRTTH
                     RATLYVSSRVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(7319..7729)
                     /locus_tag="WGO87_000812"
     CDS             complement(7319..7729)
                     /locus_tag="WGO87_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392194.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPMPNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGGRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            complement(7731..7976)
                     /locus_tag="WGO87_000813"
     CDS             complement(7731..7976)
                     /locus_tag="WGO87_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392195.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(7984..9156)
                     /gene="lpxB"
                     /locus_tag="WGO87_000814"
     CDS             complement(7984..9156)
                     /gene="lpxB"
                     /locus_tag="WGO87_000814"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0392196.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLKRYPAARFLLPAATEATKRRLAEILQRSEFAGLPLTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9204..10196)
                     /locus_tag="WGO87_000815"
     CDS             complement(9204..10196)
                     /locus_tag="WGO87_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0392197.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            10690..13449
                     /locus_tag="WGO87_000816"
     CDS             10690..13449
                     /locus_tag="WGO87_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0392198.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSENATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         3542 a         4553 c         3291 g         2677 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccggttt caccgaatcc cgcttcgtcg tcgctgccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 atgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgttttg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 acaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca ataccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aagatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaacac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcac ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgcattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcgtc
     2341 cgcgaccttg ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcac gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acgcccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccggccgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa ccctcaacaa aggaacgaac catgggcatc
     3241 aaagtcgcca tcaacggcta cggacgcatc ggccgccagg ttttgcgcgc catctacgac
     3301 tatcagattc aagaccaact ccaaatcgtc gccgtcaacg ccagcggcag ccttgaaacc
     3361 aacgcccatc tcaccaaatt cgacaccgtg cacggacgct ttgaagccga cgtatcccac
     3421 gacggcggca acctcatcgt caacggcgac aaaatcccct tcttctcgac ccgcaaccct
     3481 gccgaactgc cgtggaaaga actcggtgtc gatttggtca tggaatgcac cggcgcgttc
     3541 gccagcaaag aaaaagccaa agttcatctg gaaagcggcg cgaaaaaagt cctcatttcc
     3601 gcaccgggcg gcgacgatgt cgatgcaacc gtcgtgtacg gcgtgaacga cagcatcctg
     3661 accgccgaca tgaccgtcgt ttccaacgct tcctgcacca ccaactgcct ctcgccggtt
     3721 gccaaagtgt tgagcgaaag cgtcggcatc gtcaaaggcg cgatgaccac catccacgcg
     3781 ctgaccaacg accaaaccgt taccgacgtg cgccacaaag acctgcgccg cgcccgcagc
     3841 ggcgtggaaa acatgattcc gaccaaaacc ggcgcggcaa aagccgtcgg tttggtactg
     3901 cccgaattga aaggcaaact cgacgggctt gccatccgcg tgccgaccgt caacgtatca
     3961 ttggtagatt taagcttcca agccgcgcgc gacacgacag tcgaagaaat caacgcactg
     4021 atgaaagccg cctcggaagc aggcgcgctc aaaggcgttt taggctacaa caccctgccc
     4081 ttggtttcca tggacttcaa ccacaccacc gaagccagcc acttcgacgc aacactgacc
     4141 aaagtcgttg acggcaacat gattaaagtc ttcgcatggt atgacaacga atggggcttc
     4201 agctgccaaa tgctgaacac cgcacgccgt atgttcggac ttgaagtgcg cccgctcaaa
     4261 taagcaataa accgtcaaac aaaatgccgt ctgaaacccg atgttttcaa gttccagacg
     4321 gcatttttca ttttttggca gtgattttgc ccggctgtcg ccatttccaa ttttaatcca
     4381 ccatatttga acctgaccgg agcataagcc gccatcaaag ccccttgcac gccacttcgc
     4441 gccgcccgat ttcctctttc aacggcacgg cgttcaacat ccgcagacgt tcggcaaaat
     4501 ccgcaaacgg cagcagctcc gaaccgaaac ggcgcatatg ttccgtgtcc tgctggcagt
     4561 ctatcagttc cacacccaaa tccgccaaaa acggcacggc gcaggcaaac gcgattttcg
     4621 acgcatccgg ttgtaatgcg aacatcgatt cgccgtaaaa caccctgccg atttgcacgc
     4681 cgtaaaagcc gcccgccaac ctcgtttcac cgctttcatc gggataatgg cactcgaacg
     4741 aatgcgcgtg tcccatttcg tgcagcttca aatatgccgt ctgaaactcg ggcgcaatcc
     4801 aagttccgtc ctgattcggg cgcgccgctg ccgcacaatg cgcgaccact tccgcaaaac
     4861 agccgttgac cgcaacccga tagctgccgt tgcgcagcgt tttcgccagc gagcgcggaa
     4921 cgtgcagacg ttcgggaaca atcaccgcac gcgggccgac cgcataccaa aaaaaccaac
     4981 cgtcccgaga aaaccacgga aacacgccgt tccgatacgc ctcgagcagc cgccccgcat
     5041 ccaaatcgcc gctcacgccg accagcccgt cgcaccgggc caaagcatag gtaggatcgg
     5101 gaaaggcata attgtcaggg gcaagcagcg gaatacgcat ggcagttctc caaaaacaca
     5161 gacggcatcc aaaatcatga atgccgtctg aacaacaaac cgtccggatt taacgtttgc
     5221 ccttggcctg acagtcgctg cacacgccgt acatataaag cgcgtgatcg acgatgcggt
     5281 agccgttttc ctcagcgatt ttgtcttgca gggcttcgat ttcgggattg tggaattccg
     5341 ttacctcgcc gcacttcacg cagacgatgt ggtcatggtg gccgcctttg tccaactcat
     5401 aaaccgcctt gcccgtttca aaatgatggc gttgcagaat acccgcctgc tcaaactggg
     5461 tcagcacacg gtaaatcgtc gccacaccga tttccacacc ctcttccaac aaaatgcggt
     5521 acacatcttc cgcactcaaa tgctcttccg aatgcgtctc gaacaaatcc aaaatcttca
     5581 aacgcgggcc ggtaaccttc agaccgctgt ctttcagttg tgcaatattg ttgaattttt
     5641 ccataatatt caatacccct gtaaaacaat agacgttata atacgcaatt tcggcctgtt
     5701 tgcccactat cgcaccatag cagtttgcaa tagtaaaacc gcaccggcgg ccgatgcgcg
     5761 gacagggcgg ataccgctta atcgtatgat tatcgttcgc ttttataaaa tattcaagca
     5821 gtgttacact acccatcccg atttgcacag aaaggcattt ccgtgaacaa aaccctcatc
     5881 ctcgcccttt ccgccctcct cggccttgcc gcgtgcagtg ccgaacgcgt ttcactgttc
     5941 ccctcgtaca aactcaaaat catacagggc aacgaactcg aaccgcgcgc cgttgccgcc
     6001 ctccgccccg gcatgaccaa agaccaagtc ctgctcctgc tcggcagccc catactgcgc
     6061 gacgcattcc ataccgaccg ctgggactat accttcaaca cctcccgcaa cggcatcatc
     6121 aaagaacgca gcaatctgac cgtctatttt gaaaacggcg tactcgtccg caccgaaggc
     6181 gacgtcctgc aaaacgctgc cgaagccctc aaagaccgcc aaaacacaga caaaccataa
     6241 ggaacacaca tgacaccgct caaaatcgcc atcgccggcg caaacggccg tatgggacgc
     6301 gtattggttg aagccgtcaa caaccatccc gacaccgtcc tttccggcgc gctcgaacac
     6361 tcgggctcag aagccctcgg gctggacgca ggctacgccg tcggactcaa aaccggcatc
     6421 gccatttcag acgacgttga cgccgttctc gcacaaagcg acgtactcat cgacttcacc
     6481 cgccccgagc ccaccctcaa acacctgcaa aaatgcgttg aaaaacaagt caacatcatc
     6541 atcggcacaa caggcttcga cgatgcgggc aaagccgcta tccacactgc cgccgaacaa
     6601 acaggcatcg ttttcgccgc caacttcagc gtcggcgtca acctcacctt ccacatcctc
     6661 gacaccgtcg cacgcgtatt aaacgaaggc tacgacatcg aaatcattga aggccaccac
     6721 cgccacaaag tcgatgcccc cagcggcacc gcgttacgca tgggcgaagt catcgccggc
     6781 gcgctcggca gagacctcaa acaatgcgcc gtttacggcc gcgaaggcca caccggtccg
     6841 cgcgatccgt cgaccatcgg ctttgccacc gtccgcgcag gcgacatcgt cggcgaccac
     6901 accgccctct tcgccaccga cggcgagcgc gtggaaatca cccacaaagc cggcagccgc
     6961 atgacctttg ccgccggcgc cgtccgcgcc gcagtttggg taaacggcaa aacgggtttg
     7021 tacgatatgc aggacgtact cgggctgaac aaccgttaac ccccatacaa aatgccgtct
     7081 gaaaagatat tgttcagacg gcattttgcc gacaggctcc gtatcggcat atgtttcaac
     7141 acacgggacg atacataaag cgtcgcccta tgtgtcgtcc tgatttggaa gggattacgc
     7201 ccctcccaaa taaagtctga ttctaccgcc ccgaaggaca gatgtccgag tggcggggtt
     7261 tcaaccgaaa aggaaatacg ataaagttgc ttgcgcggca tgacaagtta cggctcgttt
     7321 aattaaaact ctcctgatct aaaaattcta actctatttc ccggcaaact atatctatac
     7381 taaaaccggt cggaagatca tacctaccgc ctatcacttc cagcgcacct ccataatcca
     7441 agatttcatc atcctgcttt aattctccaa gttccaatac atttttgata tttttatctt
     7501 ggttcttcgt ggcgtatcca tagataaatt tcagatagct gaactgcttg aatttaattt
     7561 ttacccattc tgaataattg ggcataggga tctcttgagg aaagtccgga taatccacat
     7621 tctggacgat gatatggaat tcatcgtttt tcaaagtata gtttaataga gtgccctcat
     7681 atatatacag aaattgataa tctttttttg gaatggtata agttgtcata ttattttacc
     7741 ttttcaaaaa caatgagttt aagatttttt gagtccaagt tggttctact actcttatga
     7801 aatttcagtt gtttaaactc ttcctttaac agaccattat attttaacct taatcccact
     7861 cgagccagtt ctgcttcatt tggaattcct cttaaatact tattgtttga tgtgccatta
     7921 atcacaattt ctcccccttt ttttgtaata cgtgctgctt cgggaaaata atccattaac
     7981 cgctcaacat cccgcctctt ccaaaaccgc gcgcgcggcc aaatccgccg tatctttttt
     8041 caacagcagg tgcaacgccc tgaaatcctg ttgcagcgcg gcaaccttat cggggtgttc
     8101 gtaccagtcc gccaacgccg ccgccagttt ttccggtacg gcatcatgtt gaagcagctc
     8161 cggcacagcc tccttaccca acaggatatt cggcaggcca acatgcggca ctttgatttt
     8221 gcgtttcaca taagcataag tcagcggcga aatcttgtag ctgatgacca tcggacgctt
     8281 gcacagcgcc acttccaaag tcgccgtacc gctcgttacc agcaccgcat ccgccgccct
     8341 gcacaccgtt tcagactgtc tgtcggttac cgtcagcggt aatccggcaa actccgaacg
     8401 ctgcaaaatt tccgccaaac gccgcttcgt cgcctccgtt gcggcaggca gcaggaagcg
     8461 tgcggcggga tagcgtttca acaacaataa tgccgtctga aaaaacaccg gcgccatata
     8521 gtcgatttcg ctgacgcggc tgccgggcag cagggcgaat acggggatgc cggcatccac
     8581 gcccaaagtt tgccgcgccg tttcacggtc gtcttccaag ggcataagct gcgccatcgg
     8641 atgaccgaca aactccgcac gtccgcccgc atcgagataa agctgcggct ccatcgggaa
     8701 caggcacagc acgcggttga cctgatgcac gattttgccc acgcgttccc gccgccacgc
     8761 ccacaccgac gggctgacat aatgcacggt cggaatgccc gcccgtttca gcttttccgc
     8821 cacacccaaa ttgaaatcgg gcgcatcgat accgacaaag acatcaggtt tcaacgacag
     8881 caaatcccgt accagccccc tgcgtatccg taaaatttcc ggcagccgcc tgaccacttc
     8941 gacaaagccg cgcaccgcca gccgctcctg atcataaagg ctctcgaaac cttccgcctt
     9001 catcagttcc ccgccgatgc cggtaaaccg cgcctgcggg caacgcttgc ggatggcgcg
     9061 tatcaggtgc gcccccaaca ggtcgcccga cgcttcgccg acactgacgg caatcaaagg
     9121 gcttttttta tcaaccatat tcgtctgtcc ccacatactt tcacgtcccg atgccgtctg
     9181 aaggcttcag acggcatcgg ggttcacatt ttcgtcccgt tttccaacat caccgcaaac
     9241 tgcgccaagt ccggcggcag ctccgccttc aacaccagcg gctcacccgt gagcggatgg
     9301 ttcaagtgca gctcggacgc gtgcaaaaac atccgcttca aacccaactt ctgcaaacga
     9361 cggttcgcct gataatcgcc gtagcgttcg tcgcccgcaa tcggacagcc ttgagattgc
     9421 aggtggacgc ggatttggtg cgtgcgcccc gttttcaacg tcgcccgcac caaagtcagg
     9481 tgcgacagcc cgacaccgtg caaaatgccg tctgaaaaac ggcttaacac acggaacacc
     9541 gtatgcgccg actgcccgtc cgcactgacg cgcaccatct tttcgccttg tgcgccggta
     9601 tatttgaaca ggggcagttt gacatggaaa ttgtcgtccg gcagtttgcc cacccccagc
     9661 gcaaggtaga tttttttggg gtggtcgtta cggatggctt cgtgaagttt gacgagcgcg
     9721 ctgcgtttct tcgccaccat caacaagccg ctcgtatcct tgtccaaacg atgaaccaac
     9781 tccaaatact tcgcctccgg acgggcgcgg cgcaactgtt cgataacgcc gaaactcacg
     9841 ccgctgccgc cgtggacggc aacgccggac ggtttgtcga tcaccaaaag cgcatcgtct
     9901 tcgtaaacaa cgtcaaacgc acgcgccggt acggcggcac gcctttcaga cggcatttcc
     9961 ttctccgcca cgcgcacagg cggaatccgc accgtatccc cctccgcaat acggctgtcg
    10021 ggtttgcagc gttttttgtt cagccgcacc tcgccggcgc ggataatgcg gtggatatgg
    10081 ctcttgggaa cccccttgag gatttttatc agatagttat caaggcgttg acccgcctca
    10141 tgttcggcaa cccctatcaa gctgaccgaa tctttgctta tttcgtgcgt tttcatctat
    10201 aatccgaaca tccgtttcag caaaaagcgc gcccgccgcg cctcctgaaa cggtttactt
    10261 taaaacggaa ttttatatta aaaacgcact ccgcaaagca ttttccctgc gcctgttccc
    10321 agccggcagg cgcagaacgt aatcaagttt gaattgattt tgccgtttcg gcgcggaagt
    10381 aagacggcag ccgggcccca agtcccaaac gcaaaccgcc cgaaaacaca ggcatcacga
    10441 taacaagaag aagccggcac ccatttttcc gaccataccg ctcggaacgc ggcagacaga
    10501 ccgccgcatc gatctttatt gccttcttta tccgaccctt ccgcacacgg ttcctcggaa
    10561 cgtgcatccc tgcggatttt caactcaaaa actgattacc ggttttatcc gaagacatcg
    10621 gaaaacccag ccttacggaa tgccgaaccg gaccggcgcg cccgaatgcc gcccgtccac
    10681 gaggtgatca tgaaaagaat gttattcaac gcaacgcagg ccgaagagct gcgcgttgcc
    10741 attgtcgatg gccaaaacct tttggacttg gacatcgaaa cgctgggcaa agaacagcgt
    10801 aaaggcaata tctacaaagg tatcattacc cgcatcgagc cgtcgctgga agcgtgtttc
    10861 gtcgattacg gaaccgaccg ccacggcttt ttgccgttta aagaagtctc ccgctcatac
    10921 ttccaagact acgaaggcgg acgcgcgcgc atccaagacg tgctcaaaga aggcatggaa
    10981 gtcatcgtcc aagtcgaaaa agacgagcgc ggcaacaaag gcgcggcgct gaccaccttc
    11041 atcagcctcg ccggacgcta tctggtattg atgccgaaca acccgcgcgg cggcggcgta
    11101 tcccgccgta tcgaaggcga agaacgtcaa gaactcaaag ccgctatggc gcaactcgac
    11161 attccgaacg gcatgagcat catcgcccgt accgccggca tcggccgcag cgcggaagag
    11221 ttggaatggg atttgaacta tctcaaacaa ctctggcagg cgattgaaga agcaggaaaa
    11281 gcgcatcacg acccttacct gctctttatg gaaagctcgc tgctcatccg cgccatccgc
    11341 gactatttcc gccccgacat cggcgaaatt ctggtggaca atcaagaagt ttacgaccaa
    11401 gttgccgagt tcatgagcta cgtcatgccg ggcaatatag gccgtctgaa actctacgaa
    11461 gaccacacgc cgctgttttc ccgcttccaa atcgaacacc aaatcgaaag cgcgttttcg
    11521 cgcagcgtca gcctgccctc cggcggcgcg atcgtcatcg accataccga agccctcgtc
    11581 tccatcgacg tgaactccgc acgcgccact cgcggcgcag acattgaaga caccgcgttc
    11641 aaaaccaata tggaagccgc cgaagaagtc gcccgacaaa tgcgcctgcg cgacttgggc
    11701 ggcttggtcg tcatcgactt catcgacatg gaaaacccca aacaccagcg cgatgtggaa
    11761 aacgtcctgc gcgacgcgct caaaaaagac cgcgcccgcg tacagatggg caaactctcc
    11821 cgtttcggcc ttttggaatt gagccgccaa cgtttgaaac cggctttggg cgaaagcagc
    11881 cacgtcgcct gtccgcgctg cgccggcacc ggcgtgattc ggggcatcga atccaccgcc
    11941 ctgcacgttt tacgcatcat tcaagaagaa gcgatgaagg acaacaccgg agaagtgcgc
    12001 gcccaagtgc ccgtcgatgt cgccaccttc ctgctgaacg aaaaacgcgc cgagctgttt
    12061 gcgatggaag agcgtttgga tgtcaacgtc gtcctgattc cgaacatcca cctcgaaaat
    12121 ccgcactacg aaatcaaccg catccgcacc gacgacgtag aagaagacgg cgaaccgagc
    12181 tacaaacgcg tcgccgagcc tgaagaagac gaatccgcca aaccgttcgg cggcgaaaaa
    12241 gccaaagccg cccgtcccga acccgccgtc aaaggcgtgc gccacaccag ccccgccccg
    12301 actgccgccc ccgagaaaaa aacctcttgg tgggacagct tcaaagcttg gctcaaacgc
    12361 attttcggcg gcagcgaaac ccaagccgcg cccgctgccg aaacctccga aaaacgcagc
    12421 acggcaaacc gcagcggcag ccgcgccaac aaccgccgcc aaaacccgcg ccgcagcaaa
    12481 cgcgaaggca gcaaagtaga agtccgcgaa gtggcaggca aaactgccgg acaggaagcg
    12541 cgtgccgaca aagccgaaac gcgcaacaac ggcaaccgcc gccgcaacga acgcggcgac
    12601 cgtgccgccg gacgcgccga cgaagcggaa atccaagacc gcaacataca gcctgccgca
    12661 accgttgcag atgccgcacc gtccgaaacc gaagtgcaaa ccggaaaacg ccgccgcaac
    12721 ggcagccgca gcgaacgcgg ccaaaccgcg ccggaaaccg ccaccgttgc cgaaacaacc
    12781 gttcagacag cggaaaacac gccgtccgaa ccgcataccg cagaagacaa aggcagcaag
    12841 cccaaatccg aacgcaaccg ccgcgaacgc gacagccgag atgccaaaga acgccgcgag
    12901 cgcaacaatc agcgcgaccg ccgtcaaaac ggcaaaaaac gcaatattcc gtctgccgcc
    12961 aaaatcgagc agtacctgaa tattcacgac accgccgaca aagtccgttc cgccgccgcg
    13021 cacgttttcg gcgaaaccga cgcaaacgcg ccgattaccg tcagcattgc cgatccggtt
    13081 gcagaaaggg atcttccgac agcatctccc gccgtttcaa acggcgacgc accggtttat
    13141 gatgcggcgg aaaaaatccg ccgtgccacc gccgccatcc tgcccgaagg cgcgacaccg
    13201 aaagccgaag cacaggaaat gccgtctgaa aacgcaacct ttacggctgc ggcggaacag
    13261 gcacgggaaa ccgcacaaac cggcggactc gtcctgatcg aaaccgaccc tgccgcattg
    13321 aaggcatggg cggcacaacc cgaagtccaa accggacgcg gtttgcgccg ttccgaacag
    13381 cccaagccgt ctgaagtcgc aaccgtccct gccgaagaaa tgatccaagt cgaaacccgg
    13441 caaggctgaa ccgacggcgg caaaaagagg ttctgttccg cagaacctct tttttacata
    13501 gattcggata cctgcaatgc cgtctgaaac ttcgccattc ccgtcattcc cgcaaccttt
    13561 cgtcattccc atgattcccg caacattccg tcattcccgt gaaaacggga atctagaacc
    13621 tcaaactttc ggataatctt tgaatattgc cgtcgcccaa aggcctggat tcccgcctgc
    13681 gcgggaatga cggtttagaa gttgcccgaa atctcaaaaa aaaccgaaac cgaacaagcc
    13741 agattcccgc aacattccgt cattcccgtg aaaacgggaa tctagaacct caaactttca
    13801 gataaccttt gaatattgcc gctgtccaat ggtctggatt cccgcctgcg cgggaatgac
    13861 ggtttagaag ttgcccgaaa cctcaaaaaa accgaaaccg aacaagccgg attcccgcaa
    13921 cattccgtca ttcccgtgaa aacgggaatc tagaacctct aaactttcag ataatctttg
    13981 aatattgccg ctgcccgaag gtctggattc ccacaacctt tcgtcattcc cgtgaaaacg
    14041 ggaatctaga acctcaaact ttc
//
LOCUS       DAWFBO010000038        13980 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFBO010000038 DAWFBO010000000
VERSION     DAWFBO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13980
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.038"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(105..1430)
                     /locus_tag="WGO87_000817"
     CDS             complement(105..1430)
                     /locus_tag="WGO87_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0392199.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1645..2484
                     /locus_tag="WGO87_000818"
     CDS             1645..2484
                     /locus_tag="WGO87_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0392200.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            2481..3647
                     /locus_tag="WGO87_000819"
     CDS             2481..3647
                     /locus_tag="WGO87_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0392201.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3911..5176
                     /gene="efeB"
                     /locus_tag="WGO87_000820"
     CDS             3911..5176
                     /gene="efeB"
                     /locus_tag="WGO87_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0392202.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPVHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5245..5577)
                     /locus_tag="WGO87_000821"
     CDS             complement(5245..5577)
                     /locus_tag="WGO87_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ0392203.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNKTESAAAEAAEEV
                     RDANGAVLQNGDTIILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIACKIDGSAMNL
                     KSEFVKKA"
     gene            complement(5652..7022)
                     /gene="glmU"
                     /locus_tag="WGO87_000822"
     CDS             complement(5652..7022)
                     /gene="glmU"
                     /locus_tag="WGO87_000822"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0392204.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVIGHGKEQVLNTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDASAA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTGQAQELLKAGVTLHDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     ARLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNVEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7077..7349)
                     /locus_tag="WGO87_000823"
     CDS             complement(7077..7349)
                     /locus_tag="WGO87_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ0392205.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPDQTDGSEQDVRGQNWQKPKFQNR"
     gene            complement(7342..8055)
                     /locus_tag="WGO87_000824"
     CDS             complement(7342..8055)
                     /locus_tag="WGO87_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0392206.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDEVLTRLHGMPKTQNTLSR
                     LKGRKAVFSNGPSFYVRAIVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPENTKECYA"
     gene            8114..9115
                     /locus_tag="WGO87_000825"
     CDS             8114..9115
                     /locus_tag="WGO87_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0392207.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGMGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9209..10057
                     /locus_tag="WGO87_000826"
     CDS             9209..10057
                     /locus_tag="WGO87_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0392208.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10083..10568
                     /locus_tag="WGO87_000827"
     CDS             10083..10568
                     /locus_tag="WGO87_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0392209.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLASVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10670..12238)
                     /gene="msrAB"
                     /locus_tag="WGO87_000828"
     CDS             complement(10670..12238)
                     /gene="msrAB"
                     /locus_tag="WGO87_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0392210.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRIEVRSHAADSHLGHVFPDGPQDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12383..13648
                     /gene="ftsY"
                     /locus_tag="WGO87_000829"
     CDS             12383..13648
                     /gene="ftsY"
                     /locus_tag="WGO87_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0392211.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGESIDDLRPFDARAFVDALLD"
BASE COUNT         3009 a         3577 c         4187 g         3207 t
ORIGIN      
        1 tccgtcaaat aagaaaggaa ttttgtgcct gcggtatcgc aaaacttcgc cttaatgcgc
       61 ccgattgact agggatgggc ttcagatggc attgttttgc cggtttacgg gcggtattcg
      121 ggcttcatac cgttgggtag gagctgccag acgtaacccg tggttttctg tttgccggca
      181 agttcgccgg cttcgtcgcc gtatccccaa aaataatcca cgcgcaccgc gcctttaatc
      241 gcgctgccgg tatcctgcgc cataatcagg cggttgaggg ctttgcgggt aaccggatgg
      301 gcggtggcga caaataaggg cgcgcccaag gtaatgtagt gccggtcgac tgcgccggca
      361 tattccccca tcaacggcgt gcccagtgcg ccgacgggac cgtcattgct gcttccggca
      421 agctcgcgga aaaagatata gctggggttt tgacccaaaa cttcggcgag gcgttgcgga
      481 ttttgccgca tataggcttt gatgccctgc atggaggtct gcccgagctt gaggtagcct
      541 ttgtccgcca tatagcgtcc gatggaaacg tagggatgtt cgtttttgtc ggcatagccg
      601 atgcggatgt atttgccgga cggggttttc agacggcccg agccttggat gtgcataaaa
      661 aaaagttcga cagggtcttc ggcgtaaccg agtatcggtg ctttgccgtc aagcgcgccg
      721 ccgttgattt ggttgcgcgt gtggtagggg aggaagcggc ttccttcaaa cctgcctttg
      781 attgccgttg tgcgcgcagt gatggggaat tgggagaggt cggcggtatg tgtgccgccg
      841 gtattgtcga ttgtgccgct gttttttccc gtctgcctga tgcggacaag ggcttttccg
      901 ctccgcaaac cggcaggcag ggggacggag ataaaatcgt cgggaatacc gtaaatcggg
      961 aagcgggctt gtgccgtccg cctgtcgtcg cccttcagca ccggctcgta atagccggta
     1021 accgtaccgg caaggcttcc gttgcctgca acctgccacg gcgtgaaata gcgttcaaaa
     1081 aactgttttg cctgaaagga atggacgggg gtttgaaagg cttgggcgca cacatcctgc
     1141 cagccttggc ggtttttcaa attggcgcag ccgaggcgga aggattgcag gcttttggcg
     1201 aaatcctgcg ccgcccagtg gggcagggac aggtgcggca caacggtata aacggccccg
     1261 ccgccgccga ccgtcgttcc ggcggggtcg gggatgccga ccggccggtc cgggccgttg
     1321 atgacggatg tgtcgggttg cggaaaggtt tggatgctct tgctttggca ggcggcgagg
     1381 atggcggcgg cgatgccgca cagggcggcg cggaacaggt attttttcat aatggacaat
     1441 gttgccggca gtaataaaaa aaaaagatgg tttcgggcgg cgttgcggca gccgtggaga
     1501 ggggatttta acacagggcg cagctgcagc ctgcggaact ttccgccgcg cggcaatgca
     1561 gataaaaata acttgcattt gtatttacaa gcaatgaaaa tattccgata atatattatt
     1621 catcatcctt gttcgttcgc gtttatgctg gtcgcttttt taattatgtt gcgcgagggt
     1681 atcgaagccg cgctcatcgt cggcatcgtt gccggttttc tgaaacagtc cggacattcc
     1741 aaactgatgc ctaaggtctg gttcggggtc gtccttgctt ctttgatgtg tttgggcatc
     1801 ggatacggca tccattcggc aacgggcgag attccccaga agcagcagga gttcgtcgtc
     1861 ggcattatcg gtttggttgc tgtcgcgatg ctgacttata tgattttatg gatgaaaaag
     1921 gcggcgcgtt cgatgaagcg gcagcttcag gattctgtac aggcggcttt gaaccgtggc
     1981 agcggtcaag gatgggcctt ggtcggtatg gcgtttcttg ccgtggcgcg cgaaggtttg
     2041 gagagcgttt ttttcctgct tgccgtattc aaacagagcc cgacgtggca gatgccggcc
     2101 ggtgcggtag cgggggtttt ggctgccgcc gtgattggcg cgttgattta tcagggtggg
     2161 atgcgcctga atctggctaa gtttttccgt tggacggggg cgtttttgat tgtcgttgcc
     2221 gccggcctgc ttgccggctc gctgcgcgcg ctgcacgaag caggcatttg gaacgcgctt
     2281 caggacattg tgttcgactc atcaaaatat ttgcacgaag acagtccgtt aggtgtgctg
     2341 ctcggcggat ttttcggcta taccgaccat ccgacgcagg gcgaggcctt ggtttggctg
     2401 ctgtacctta ttcccgtcat gacttggttt ttgtgcggca gcaggccgtc tgaaacttta
     2461 acccgtaaag aggagctgaa atgagaaaat tcaatttgac cgcattgtcc gtgatgcttg
     2521 ccttaggttt gaccgcgtgc cagccgccgg aggcggagaa agctgcgccg gcagcgtccg
     2581 gtgaggcgca aaccgccaac gagggcggtt cggtcagtat cgccgtcaac gacaatgcct
     2641 gcgaaccgat ggaactgacc gtgccgagcg gacaggttgt gttcaatatt aaaaacaaca
     2701 gcggccgcaa gctcgaatgg gaaatcctga aaggcgtgat ggtggtggac gagcgcgaaa
     2761 acatcgcccc cggactttcc gataaaatga ccgtcaccct gttgccgggc gaatacgaaa
     2821 tgacttgcgg tcttttgacc aatccgcgcg gcaagctggt ggtaaccgac agcggcttta
     2881 aagacaccgc caacgaagcg gatttggaaa aactgtccca accgctcgcc gactataaag
     2941 cctacgttca aggcgaggtt aaagagctgg tggcgaaaac caaaaccttt accgaagccg
     3001 tcaaagcagg cgacattgaa aaggcgaaat ccctgtttgc cgacacccgc gtccattacg
     3061 aacgcatcga accgattgcc gagcttttca gcgaactcga ccccgtcatc gatgcgcgtg
     3121 aagacgactt caaagacggc gcgaaagatg ccggatttac cggctttcac cgtatcgaat
     3181 acgccctttg ggtggaaaaa gacgtgtccg gcgtgaagga aattgcagcg aaactgatga
     3241 ccgatgtcga agccctgcaa aaagaaatcg acgcattggc gtttcctccg ggcaaggtgg
     3301 tcggcggcgc atccgaactg attgaagaag tggcgggcag taaaatcagc ggcgaagaag
     3361 accggtacag ccacaccgat ttgagcgatt tccaagccaa tgtggacggt tcgaaaaaaa
     3421 tcgtcgattt gttccgtccg ctgatcgagg ctaaaaacaa agccttgttg gaaaaaaccg
     3481 ataccaactt caaacaggtc aacgaaattc tggcgaaata ccggactaaa gacggttttg
     3541 aaacctacga caagctgggc gaagccgacc gcaaagcgtt acaggcctct attaacgcgc
     3601 ttgccgaaga ccttgcccaa cttcgcggca tactcggctt gaaataagcc gcaagcgttc
     3661 agacggtatt tggcggcaga taccgtctga agttttatag tggattaaca aaaaccagta
     3721 cggcattgcc tcgccttgcc ttgccgtact atttatactg tctgcggctt cgtcgccttg
     3781 tcctgatttt tgttaatcca ctatatccgc catatattgc agggcaggat ttcaacctgc
     3841 cgctatcggt taatggaaaa acggcgtgca gggataccca tcctgctgca cggatattga
     3901 aggaaacacc atgagcaaaa accaacccgc acaaccgacc aggcgcactc tttttaaaac
     3961 cgcgatcgca gccggagcag tcggcgcaat cggaggttat ctcggcggca aaaaacaggg
     4021 cgaaaccgcc gaacgcaccg ccgaaagcca acactcgccc caagcctatc cctgctacgg
     4081 cgaacatcag gcaggcatcg ttacgccgca gcaggcgttt tcgattatgt gcgccttcga
     4141 cgtaaccgcg caaagtgcca agcagctgga aaacctgttc cgcacgctga ccgcccgcat
     4201 cgagtttctc acccaaggcg gcgaatacca agacggcgac gacaaacttc cacccgccgg
     4261 cagcggcatt ttgggcaaag ccttcaaccc cgacgggttg accgttaccg tgggggtggg
     4321 cagcagcctg tttgacggcc ggttcggact caaagacaaa aaaccggttc atttgcagga
     4381 aatgcgcgac ttctccaacg ataagctgca aaaaagctgg tgcgacggcg atttgagcct
     4441 gcaaatctgc gccttcaccc ccgaaacctg ccaagccgcc ctgcgcgaca tcatcaaaca
     4501 caccgtccaa accgccgtta tccgttggag tatcgacggg tggcagccta aatccgaacc
     4561 cggcgcgatg gcggcgcgca acctgttggg cttcagggac ggcacgggca accccaaagt
     4621 ttccgacccc aaaactgccg acgaggtttt gtggacgggt gtggccgcca acagcctcga
     4681 cgaaccggag tgggcgaaaa acggcagcta tcaggcagtc cgccttatcc gccactttgt
     4741 cgagttttgg gacaggacgc cgcttcaaga gcaaaccgac attttcgggc ggcgcaaata
     4801 cagcggtgcg ccgatggacg gcaaaaaaga agccgaccaa ccggattttg ccaaagaccc
     4861 cgaggggaat accacgccca aagacagcca tatacgcctg gcgaatccgc gcgatcccga
     4921 attcctcaaa aaacaccgcc tcttccgccg cgcctacagc tattcgcgcg gactcgcctc
     4981 aagcggacag cttgatgtcg ggctggtgtt cgtctgctat caggcaaacc ttgccgacgg
     5041 attcatcttc gtgcaaaacc tcctcaacgg cgaaccgctg gaagaataca tcagcccctt
     5101 cggcggcggc tatttcttcg tgctgcccgg cgtggaaaaa ggcggctttt tggggcaagg
     5161 gctgctgggc gtataaatcc gccatataaa aaacgccgtc tgaatccgat aaggggttca
     5221 gacggcattt tactgtttgg gcggtcaggc ttttttgacg aattcagatt ttaaattcat
     5281 cgcgctgccg tcgattttgc aggcgatgtt gtgatcgcct tcctgcaggc gtatgccttt
     5341 gactttcgta ccttgtttga tcgtcatcga gctgcctttt accttgaggt ctttgatgag
     5401 gatgatggtg tcgccgtttt gcagcaccgc gccgttggca tcgcgcactt cttcggcggc
     5461 ttcggctgcg gcggattcgg ttttattcca ttcgtgggcg cattcggggc agatgtattg
     5521 tccgccgtct tcgtaggtgt attcggaggc gcattgcggg caagggggta atgacatggt
     5581 ttgccgtcct tatcggatgt ttgttttggg gtgccgtctg aaacctgaaa ccggcttcag
     5641 acggcatagc tttattgttt gtctttttca ggacgcaccc agccttcgat gacggtttgg
     5701 cgggcgcggg cgagggcgag tttgttgtct tcgacattgc gggtaatcgt gctgcccgcg
     5761 cctgtggtta ctttgttgcc gagggtaacg ggggcgacta ggacgcagtt tgaaccgatg
     5821 cgcacttcgt cgccgatgac ggttttgtgt ttgtgcacgc cgtcgtagtt ggcaataatc
     5881 gtaccggcgc cgaagttggt tttgctgccg acttcggcat cgccgatgta ggtaaggtgg
     5941 ttggctttgg tgcctttgcc gatggcggcg tttttgattt cgacgaagtt gccgacgtgt
     6001 acgtcgtctg caaggcgggc ttgcggacgc aggcgggcgt acgggccgat tcggttgttt
     6061 tcgccgactt cgcagtcttc gaggtgggag aagggggcga ttttgctgtt tgcaccgatt
     6121 ttggcgtttt tgatgacgca gtttgcgccg atttcgacgt tgtcgccgat ttcgacttcg
     6181 ccttcaaaga tacagttcac atcaattacg acatcctgtc cgtgtttcag acggcctcgt
     6241 aaatcgaaac gtgccgggtc gtgcagggta acgcctgctt tgagcaattc ttgcgcctgt
     6301 ccggtttgga agatgcgttc gagttcggcg agctggcgtt tgttgttcac gccggcggcg
     6361 aggtgggagg cgcggacttg gacgggatga actttaatac cgtcggcaac ggctttggcg
     6421 atgaggtcgg tcaggtagta ttcgccttgg gcattgttgc tggaaaggct gttcagccag
     6481 ttttccagtt tggcgttggg caggacgaga atgcctgtgt tggtttcttt cacggctttt
     6541 tgggcggcac ttgcgtcttt ttcttcgaca atggcggtta cgctgccgct gccgtcgcgg
     6601 atgatgcgcc ccaaacctga cgggtcggcg ggcacgtcgg tcaacagccc gacttcgttg
     6661 cctgcggctt cgagcagggt ttcgagggtt tcaacgtcaa ttaaaggaac gtcgccgtac
     6721 aacaccagcg tgcggccttc ggcggcaagg tggggcaggg cggttttgac ggcgtggccg
     6781 gtgccgagct gttcggtttg ttcaacccaa acgacatcgc gtttgacagt attcaaaact
     6841 tgctctttgc cgtgtccgat gacgacgcag atgttttgcg gattcagtgc ggcggcggtg
     6901 tcgataacgc gctcaaccat gggcttgccg ccgatgcagt gcagcacttt gggcattttg
     6961 gaatacatgc gcgtgccttt gccggcggcg aggataacga tgtttaaagt gttttgcggc
     7021 atgacggttt cctgtgcaat gccgtctgaa agggcttcag acggcatagg gtgggtttat
     7081 cggttttgaa atttgggctt ttgccagttc tgtccgcgta cgtcttgttc ggaaccgtcg
     7141 gtttggtcgg gcagcacggc atggcgttcg gggcggtatt ggttgtagtt catatttttc
     7201 gagtagctgc cgtcttggta ataaacgggc gtgccggcgg gatatttttg acggacggcg
     7261 gtcttgccgt tgccgtcttg ataagtttcc cacgcgcagc ccgacaaaag agcggcggca
     7321 gtcatcagga taaggaaggt tttacgcata acattctttc gtattttcgg gggttgtgcg
     7381 agtattgtaa tgattttggc ggtgttctga caaagtttct gcataccgag ccagttgcgc
     7441 catatcgctt acggaagcat cgataaaggg caggtcgtgc acttttgcgc cgaaccggac
     7501 ggttttcatg cccaatgctt ttgcctgatg caggttgtcc gcgctgtcgt ccaccataat
     7561 gcagcattcg ggcggtacgt ccaaaaggcg gcagacattg agatacgctt gcggattggg
     7621 tttgtacagc agtccgaaat catctgtgcc gaacagtgcg tcgaaacggt tttccaaacc
     7681 gagtgcgccg acaatggcgc ggacgtaaaa cgacgggccg ttggaaaaaa ccgccttgcg
     7741 cccttttagg cggctcaggg tgttttgtgt tttcggcata ccgtgcagac gggtcaaaac
     7801 ctcgtcaatc ggatggcttt cgcgcaaaaa ttcggcgatg tcgatttcgg gatggtggat
     7861 ttgcagtccg gcgagcgttg cgccgtagcg gtgccaatag tcttgacgca ggtcggacgc
     7921 ggcggattcg gagagtttga ggcggcgtgc catatagcgt gtcatggcgc ggttgatgag
     7981 tgtgaagatg ccggcatcgg cattgtgcag cgtgttgtcg aggtcgaaca gccacacggt
     8041 cgggttttct tgcattttga accgtgaaaa tttgttagaa tgttatttta cagcgaatag
     8101 aggaggactc ggaatgaaac ggaaaatttg gctgctgccg ctgctggcgg tttcggcata
     8161 cctgcaggcg cagacggaag tcaggctggc ggtgcataag tcgttcagcc tgcccaaagg
     8221 gctgattgcg cgcttcgagc gggcaaacga tgcgaaggtg tcgattattc aggcgggcgg
     8281 cgcgaacgaa atgctcaaca aactgatttt gagccgcgcc aacccgattg ccgacgcggt
     8341 gtatggtttg gacaacgcca atatcggcaa ggcgcgggaa atgggcattt tggcggcggc
     8401 gcaacccgaa tccgcccccg tcgcggtcgg gctgccttcg gctttggcgg tcgattacgg
     8461 ctatgtgtcc atcaattacg acaaaaaatg gtttgaaggc aaaaagctgc ccctgccgca
     8521 aaccctgcag gatttgaccc gccccgaata taaaaaccta ttggtcgtgc cgtcccccgc
     8581 cacgtcgtcc ccggggctgg gcttcctgat ggcgaacatc agcgggatgg gcgaagaagg
     8641 cgcgttcaaa tggtgggcac agatgcggca gaacggcgtg aaggtcgcca aaggctggag
     8701 cgaggcgtat tacaccgact tttcgcacaa cggcggcgcg tatccgctgg tggtcggtta
     8761 tgccgccagt ccggcggcgg aagtgtattt ttccaaaggc aaatacagcg agccgccgac
     8821 gggcaacctg tttttaaaag gcggcgtatt ccgccaggtc gaaggcgcgg cagtcttgaa
     8881 gggcgcgaaa cagccgaagc tggcggcaaa actggtgcaa tggctgcaaa gtcgggaagt
     8941 gcagcaggcg gttccgtccg aaatgtgggt ttaccccgcc gtcaaaaaca cgcgcctgcc
     9001 cgacgtgttc cgcttcgccc aagccccgac gcacaccacc gcccccgcgc agcgcgatat
     9061 tgatgcgaac cagcgcggat gggtttcccg ttggattaga acggttttga aataaaacaa
     9121 acataccgcc ccgcagggct tcatacggca tttttacacc tgtgccgatt acgccgcacg
     9181 gggcggatgt tcgatcaaga ggaaaacaat ggacttcaaa caatttgatt ttttacacct
     9241 gatcagtgtt tccggttggg agcatctggc tgaaaaggcg tgggcgttcg ggctgaacct
     9301 tgccgccgcg ctgcttattt ttttggtcgg aaaatgggcg gcgaaacgca ttgtcgctgt
     9361 gatgagggcg gcgatgacgc gcgcgcaggt cgatgccacg ctgattagtt ttttgtgtaa
     9421 tgttgccaat atcggcttat tgattttggt gattattgcc gcattgggca gattgggcgt
     9481 ttccacaaca tccgtaaccg ccttaatcgg cggcgcgggt ttggcggtgg cgttgtcctt
     9541 gaaagaccag ctgtccaatt ttgccgccgg tgcgctgatt atcctgttcc gcccgttcaa
     9601 agtcggcgat tttatccgcg tcggcggttt tgaaggatat gtccgagaga ttaaaatggt
     9661 gcagacttct ttgcggacga ccgacaacga agaagtcgtg ctgcccaaca gcgtggtgat
     9721 gggcaacagc atcgtcaacc gttccaccct gccgctttgc cgcgcccaag tgatagtcgg
     9781 cgtcgattac aactgcgatt tgaaagtggc gaaagaggcg gtgttgaaag ccgccgtcga
     9841 acaccccttg agcgttcaaa acgaagagcg gcaggccgcc gcctacatca ccgccttggg
     9901 cgacaatgcc atcgaaatca cattatgggc ttgggcaaac gaagcagacc gctggacgct
     9961 gcaatgcgac ttgaacgaac aagtggtcga aaacctccgc aaagtcaata tcaacatccc
    10021 gttcccgcaa cgcgacatac acatcatcaa ttcttaaacg ccgtctgaaa ggggagtggg
    10081 aaatggacgc gctgcacacc atcgcccgaa acctgacgaa aaaacgtcaa accgtaagct
    10141 gtgccgaatc ctgcacgggt gggctgcttg cctccgtatt cacaagcgtt gcaggcagtt
    10201 cgcaatggtt cgatcaaagt tttgtaacat acagcaacaa agccaaagaa gaccgcttgg
    10261 gcgtgttgcc cgaaaccctg ctcgaacacg gcgcggtcag ccgccaaacc gtctatgaga
    10321 tggcgcgcgg cgcgaaagcc gtggcgcagg cggattacgc cgtcggtatt tccggcatcg
    10381 ccggtccggg cggcggcagc gaaagcaaac ccgtcggcac ggtttggttc gggtttgcct
    10441 ttccgggcgg aagttgcgaa gcaatgcgcc gttttgacgg caaccgcgaa tccgtccgcg
    10501 cgcaggcggt cgccttcgcg ttggaacggt tggcggggct gattgaaaac ggcggcgatg
    10561 ccgtctaaac aaaatctccg tctggacaaa atccccgccg gataaaaaat accgtctgaa
    10621 acgttgcagg tttcagacgg tattttgtcg ggttaggcgg cggtgcggct tatttcactt
    10681 tactcttcaa cgcgccgtag cctgccgcgt ccatttgttc cagcgggatg aatttcaaac
    10741 tcgcgccgtt gatgcagtag cgcagaccgc ctttgtcttg agggccgtcg gggaagacgt
    10801 gtcccaagtg cgaatcggcg gcgtggcttc tgacttcgat gcggcgcata ttgaagctga
    10861 aatcatcgtg ttcggtaacg gattttgcat caatcgggcg cgtgaagctc ggccagccgc
    10921 agccggaatc atatttgtcg gcggagctga acaaaggttc gccgctgaca acgtccacat
    10981 aaatgccggg tttgaacaaa tggtcgtatt cgtggctgaa ggcatattcg gtcgcgctgt
    11041 tttgggtaac ttggtattgc tcttcggtca gggtgcgttt gagttcggcg tcactcggtt
    11101 ttttatacgt tgccgcatcg aagcctttgc cttgcggggc ggctttggtt ttgcccggca
    11161 gcggttcgtc ggctttgcgg atatcgatgt ggcagtagcc gttggggttt ttaatcaggt
    11221 agtcctgatg gtattcctcg gcatcgtaga agtttttcag cggctcgttt tcaacaacga
    11281 ggggcagttg gtatttttgc tgctcgcgtt tgagggcggc ggcgatgacg gctttttcgg
    11341 cggggtcggt gtagtacacg ccgctgcggt attgcgtacc ggtgtcgttg ccctgtttgt
    11401 tgaggctggt cggatcaacg acgcggaaga aatattgcag gatgtcgtct aggctgagtt
    11461 tgtcggcatc gtaggtcact ttaacggttt cggcgtggcc cgtatggcgg taggacacgt
    11521 cttcatagct cggatttttc gtgttgccgt tggcgtagcc ggataccgcg tcaaccacgc
    11581 cgtcgatgcg ttggaaatag gcttccaagc cccagaagca gccgccggcg aggtaaatgg
    11641 tgcgcgtgtt catgattttt gaatcctttt tctgagtgtc gggtttgtag aacgaatgtt
    11701 tcaagctgcc caaatcggca ttcgggtcgc ggattaacgc caacgcctgc gcttcgttga
    11761 tgctgccttt gacgatgcgc tgcacgtcgc cgtctttacc gattaacgcc cacgaagggt
    11821 aaacgctgat attcaggctt tgggcgatcg tgccgccgtt gtcggttacg acgggcagct
    11881 tgggataatt caaaccggca taccattttt ggaagtcgcc gtcttttttc tcgtgcaaaa
    11941 agcccggaga cgcgacggta atcaggttgg cggagctgaa ttttgcatct tgcgcccatt
    12001 tttcggtctg tcccaattcg gacagacaca aaggacacca gctcgcccaa aatttaatca
    12061 gcgtcggttt gtcttttttc aagtaaacac tggcggggcg gttgtccgca gttttcaaag
    12121 tggataaagt gtgcggcacg gtcgcggctc cggcatcgac gattttgggc gaacaagcac
    12181 ccagcgcaag caggcagccg aacttggcgc aaagggaaaa gaaagtacgg tgtttcattt
    12241 tgagtttcct gtgtggacgg tttgcatgat tagacgtttg agatgccgaa accttacagc
    12301 tcggattttc agacaacctt gccgcgtaaa atacgctaca atacgccctg tttcaagttt
    12361 ctaaaattaa aaggaaaatt caatgttcag cttcttccgt cgcaagaaaa aacaggaaac
    12421 gccggctctc gaggaggctc aaattcagga aaccgcagca aaagcagagt ccgaaaccga
    12481 tcaagtaatt gaaaatatta aagaagatgc tgaatcttta gcagaaagcg tcaaagggca
    12541 ggtcgaatct gccgttgaaa ccgtcagcgg tgcggttgaa caggtaaagg aaaccgttgc
    12601 cgagatgctg tctgaagcag aggaagcggc ggaaaaagca gcggaacaag tcgaagcggc
    12661 aaaagaagcc attgccgaaa ccgtcggcga ggctgtcggg caagttcaag aagccgttgc
    12721 aacaactgaa gaacacaagc tcggttgggc ggcgcgtttg aaacaaggtt tgaccaaatc
    12781 gcgcgacaaa atggcgaaat cgctggcggg cgtgttcggc ggcggacaaa tcgacgaaga
    12841 tttgtacgaa gagctggaaa ccgtgctgat taccagcgac atgggcatgg aagccaccga
    12901 atacctgatg aaagacgtgc gcgaccgtgt cagcctcaaa gggctgaaag acggcaacga
    12961 attgcgcggc gcgttgaaag aagccttgta cgacctgatt aagcctctgg agaaaccttt
    13021 ggttttgccc gaaaccaaag agccgtttgt catcatgctt gccggcatca acggcgcggg
    13081 caaaaccacg tctatcggca aactcgccaa atatttccaa gcgcagggca aatctgtgct
    13141 gcttgccgcc ggcgatactt tccgcgccgc cgcccgtgag cagcttcaag cttggggcga
    13201 acgcaacaac gtaaccgtga tttcgcaaac cacgggcgat tccgccgccg tgtgcttcga
    13261 tgccgtccaa gccgccaaag cgcgcggcat cgacattgtg ctggccgaca ccgccggccg
    13321 cctgcccacg cagcttcatt tgatggaaga aatcaaaaaa gtgaaacgtg tgctgcaaaa
    13381 agccatgccc gacgcgccgc acgaaatcat cgtcgtgctt gatgccaaca tcgggcaaaa
    13441 cgccgtcaac caagtcaaag cctttgacga cgcattaggg ctgaccggtt taatcgtgac
    13501 caaactcgac ggcacggcaa aaggcggcat cctcgccgcg cttgcttccg accgccccgt
    13561 ccccgtccgc tacatcggcg tgggcgaaag catagacgac ctgcgcccgt ttgacgcgcg
    13621 cgcgtttgtg gacgcactgc tggattgagc cgaaatgccg tccgaaaaca gcagaccgat
    13681 gccgtcattc ccgcgcaggc gggaatctag accttgggat aacagcaata ttcaaaggtt
    13741 ataaaagacc cgtcattccc gcgcaggcgg gaatctagaa cgtaaaatct aaagaaaccg
    13801 tgttgtaacg gcagaccgat gccgtcattc ccgcgtaggc gggaatctag gccattggac
    13861 agcggcaata ttcaaagatt atctgaaagt ccgagattct agattcccac tttcgtggga
    13921 atgacgggat gtaggttcgt gggaatgacg cggtgcaggt ttccgtgcgg atggattcgt
//
LOCUS       DAWFBO010000039        13539 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFBO010000039 DAWFBO010000000
VERSION     DAWFBO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13539
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.039"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..79)
                     /locus_tag="WGO87_000830"
     CDS             complement(<1..79)
                     /locus_tag="WGO87_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0392212.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WGO87_000831"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WGO87_000831"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0392213.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPDLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERIRFTTSHPREFTDAIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGO87_000832"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WGO87_000832"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0392214.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVVDMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDGLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGO87_000833"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WGO87_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0392215.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQGLSQRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDSLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /locus_tag="WGO87_000834"
     CDS             4804..5868
                     /locus_tag="WGO87_000834"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0392216.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     ISLEKMATKYAKGELNQIVK"
     gene            6027..6848
                     /locus_tag="WGO87_000835"
     CDS             6027..6848
                     /locus_tag="WGO87_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ0392217.1"
                     /translation="MNRTTFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLD
                     HKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQLITLESGEFQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAF
                     NQLPSGKAEYHGKAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVELASAELKA
                     DEKSHAVILGDTRYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            6987..7664
                     /gene="tsaB"
                     /locus_tag="WGO87_000836"
     CDS             6987..7664
                     /gene="tsaB"
                     /locus_tag="WGO87_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0392218.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETRLFHQEVGSRQSELI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASMPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADITLPEGHVFSDG
                     IGNAFALENRPPFAGKPDMPTAADFLNLAEEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            7661..8101
                     /gene="rimI"
                     /locus_tag="WGO87_000837"
     CDS             7661..8101
                     /gene="rimI"
                     /locus_tag="WGO87_000837"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0392219.1"
                     /translation="MNIRRPSPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRLAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEGTQRLLLE
                     VRAGNIGAQTLYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8095..8832
                     /locus_tag="WGO87_000838"
     CDS             8095..8832
                     /locus_tag="WGO87_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0392220.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8894..9535
                     /gene="pyrE"
                     /locus_tag="WGO87_000839"
     CDS             8894..9535
                     /gene="pyrE"
                     /locus_tag="WGO87_000839"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0392221.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9609..10043
                     /locus_tag="WGO87_000840"
     CDS             9609..10043
                     /locus_tag="WGO87_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392222.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10040..11350
                     /gene="argA"
                     /locus_tag="WGO87_000841"
     CDS             10040..11350
                     /gene="argA"
                     /locus_tag="WGO87_000841"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0392223.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFHLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AVTLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11446..13461
                     /locus_tag="WGO87_000842"
     CDS             11446..13461
                     /locus_tag="WGO87_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0392224.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARTLS
                     DGILAQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCAAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPDRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPADCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGASWTDEYGNPQKYEVCKCRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
BASE COUNT         3023 a         4222 c         3597 g         2697 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatac agccgtcctg
      121 accgattaaa ttcatatttt aaaacaatat cctgcctcca aaacccacat cgtgctataa
      181 tccacaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gtaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcacagg aaaaagtgtt ctccgacttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaat cccgacctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtcgtcttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgacc gaaatcgcca
      841 accttgccca gcaaggtgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgt atcgtccacg
      961 aaatccccgg catcgaacgt atacgcttca ccaccagcca tccgcgcgaa tttaccgacg
     1021 caattatcga gtgctaccgc gacctgccca agctggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgcgccatcc gtcctgattt gtgcctcagc tccgacttta
     1201 tcgtcggctt ccctggcgag accgagcgcg agttcgagca aaccttgaaa ctggtcaaag
     1261 acatcgcctt cgacttgagc ttcgtcttta tttacagtcc gcgccctggc acgcctgccg
     1321 ccaacctgca cgacgacaca ccgcatgaag aaaaagtgcg ccgcctcgaa gctttgaacg
     1381 aagtcatcga agccgaaacc gcgcgtatca accaaaccat ggtcggcacg gttcaacgct
     1441 gcttggtcga aggtatctcc aaaaaagacc ctgaccaact gcaagcccgt accgccaaca
     1501 accgcgtggt caactttacc ggcacacccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaaattgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgcag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa acccaaaccg tccaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcgacga cggtggcgtt cagttttccg gcgaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg catccgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggc
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caatgccgac atcgaaataa cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat cgcgggcgtg atggcgatca aacgctcgtc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcgac gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgcct tctttaggca gcttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcagc gagtttgtag ccgttgcctt ttttggtgat gacgtgcaga
     2761 agctgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt gtagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggatgtgg aagaatgccc tacgccgaac gcgtcgtatt ccgactcgct gcgttttggg
     3301 aagccagcta aaccgccgta ttggcgcatg gtgtgcatct ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccatacca gcttgtcttc gggcgtatcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca aattgctggc gaaatgcccg
     3481 ccggtctgcc cgacagattc cagcagaaag gcgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggccaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gcttggtata aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccggtcagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagt tcgaacagcg cgtgatcgcg caccgccagc
     4201 gagtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt tgaccagcca ggcgcaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgttgc
     4441 gacaagccct gcggcacgcc gccttcagct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgct ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cccccgtcat cgttcaagca tccgccggcg cgcgcaaata cgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggct gtcgaagaat tcccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atctcctgat gtatgccaac gctccatcca actgggtttt
     5101 tcttcggtaa tgatggacgg ctcgctgctc gaagacggta aaaccccttc ttcttacgaa
     5161 tacaacgtca acgccacccg taccgtcgtt aacttctccc acgcctgcgg cgtgtctgtc
     5221 gaaggcgaaa tcggcgtatt gggcaacctc gaaaccggcg aagccggcga agaagacggc
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctga ccagcgtcga agatgccgta
     5341 cgtttcgtta aagataccgg cgttgacgca ttggctattg ccgtcggcac cagccacggc
     5401 gcatacaaat tcacccgtcc gcccacaggc gacgtattgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gctccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgacaccgac
     5641 ttgcgccttg cgtccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttt
     5701 gacccacgca aatacctgag caaaaccatt gaggctatga agcaaatctg cctcgaccgt
     5761 tatcttgcgt tcggttgcga aggtcaggca ggcaaaatca aaccgatttc tttggaaaaa
     5821 atggcaacca aatacgccaa aggcgaattg aaccaaatcg tcaaataaca ggttgcctgt
     5881 aaacaaaatg ccgtctgaac cgccgttcgg acggcatttg atttttgctt ctttgacctg
     5941 cctcattgat gcggtatgta aaaaaagata caataactag ggtgtttata tattatctat
     6001 atctgcgtat gactaggagc aaacctgtga accgaactac cttctgttgc ctttctctga
     6061 ccgccgccct gattctgacc gcctgcagca gcggaggggg cggtgtcgcc gccgacatcg
     6121 gcgcggggct tgccgatgca ctaaccgcac cgctcgacca taaagacaaa agtttgcagt
     6181 ctttgacgct ggatcagtcc gtcaggaaaa acgagaaact gaagctggcg gcacaaggtg
     6241 cggaaaaaac ttatggaaac ggcgacagcc tcaatacggg caaattgaag aacgacaagg
     6301 tcagccgctt cgactttatc cgtcaaatcg aagtggacgg gcagctcatt accttggaga
     6361 gcggagagtt ccaaatatac aaacaggacc actccgccgt cgttgcccta cagattgaaa
     6421 aaatcaacaa ccccgacaaa atcgacagcc tgataaacca acgctccttc cttgtcagcg
     6481 gtttgggtgg agaacatacc gccttcaacc aactgcccag cggcaaagcc gagtatcacg
     6541 gcaaagcatt cagctccgac gacccgaacg gcaggctgca ctactccatt gattttacca
     6601 aaaaacaggg ttacggcaga atcgaacacc tgaaaacgcc cgagcagaat gtcgagcttg
     6661 cctccgccga actcaaagca gatgaaaaat cacacgccgt cattttgggc gacacgcgct
     6721 acggcggcga agaaaaaggc acttaccacc tcgccctttt cggcgaccgc gcccaagaaa
     6781 tcgccggctc ggcaaccgtg aagataaggg aaaaggttca cgaaatcggc atcgccggca
     6841 aacagtagca tttgaaaaat gccgtccgaa cacgatagtt taccgttcgg acggcatttt
     6901 tgcgccatac tgccctagga cacgacggca cggcaggcaa gctgatttca ctatataatg
     6961 ccgtctgaac caacgagaac cataccatgc aagctgattt taaccgtccc gtcctggcca
     7021 tcgataccgg tacttcctat ttgtcgctcg cgctgcgcgc cgacggcgaa acccgtctgt
     7081 tccatcagga agtcggcagc cgccagtccg aactgattct gccggaaatc cgcacgttgt
     7141 ttcacgatgc aggcattacc gccgccgatt tgggggcgat tgtttatgcg aaaggtcccg
     7201 gcgcgtttac gggactgcgt atcggcatcg gcgtggcgca aggtttggca acgccgtttg
     7261 atacgccttt aatcggcatc cccacgctcg atgccgccgc ctcgatgccg ccgccgcaaa
     7321 gctgcatact cgccgctgcg gacgcgcgta tgggcgaagt gttttatgcg tggttcgaca
     7381 cgctgaaccg ccgccgtttg agcgattatc aagtcggtag ggcggcagac attaccctcc
     7441 ccgagggaca cgtcttttca gacggcatcg gcaatgcgtt cgcgctggaa aaccgtcctc
     7501 ccttcgcagg taaacccgat atgccgactg ccgccgactt tctcaatctg gcggaagaag
     7561 gcggttttcc cgccgtcgct gcggcacacg ccgaactgct ctacgtccgc aacaaaatcg
     7621 ccttgactgc caaagaacag gcggaacgga aagcccgaac atgaacatcc gccgcccctc
     7681 accgtccgac tgcgccgcac tcgccgcgct cgatgccgtc tgcaacccgt ccgcgtggac
     7741 gcaacgccaa tttgagtccg cactggtttc gccgtccgaa caggttttcc ttgcggaaaa
     7801 agacggcagg ttggccgcct ttatcgtttg gcagaacctg cccgacgaat ccgaactgca
     7861 cctgattgcc accgcgcccg aatgccgccg ccggggtgtt gcgtccgccc tgctcgaata
     7921 ctggttcgca catctgcccg aaggcacgca acgcctgctg ctcgaagtcc gcgcgggcaa
     7981 tatcggcgcg cagacgctgt acgcggcgca cggcttcagc attgcgggca ggcggaaaaa
     8041 ctattaccgt acagccgacg gtaaaaccga agatgccgtc ttgatggaga aaatatgtta
     8101 agcgcgcgct acctccacct gcacgaagct ttgggtttag gcccgatgtg gctgaaacag
     8161 gccgccgccg tcctgccgcc caaaaacaca cccgcaaccc cggcacaggc acgtcctcaa
     8221 accgtccgcg ccgccccgat ccgcccttcc caaccccata acggtcaggc gcggctcgaa
     8281 acgatgaaag cgttggaaac cgccgccgta cctacgcgca aacccgcgcc tgaaaccgaa
     8341 acgcctctgc ccggcctttc agacggcatc gcccccgttc ccgccgcttc gggcatcacc
     8401 aagcttgccg tcgtcagcct ttgcccaccg atcgaggatg cggtttacgg gcaactgttc
     8461 tacggcaaag cgggtgtcct gcttgacaat atactcaaag ccgtaggact ggatgccgcc
     8521 tatgtccaca aaacctgttg ggtgaaaacc gccgccgtcg gcaacccgat gccgtctgaa
     8581 gcggcaatcg aaaccgcgac ggtttccgtc atgcaggaac tcgacggctg ccgcgccccc
     8641 gccgtcctat tcctcggaca ggcttttgtc cagcctgaac ggcaaacgat gattgaaact
     8701 ttgtgcggca gccgtccctt cttcatcatc gaccatcccg cccggctgtt gcgccaaccc
     8761 gaacttaaag cccgcgcctg gcaggtgttg aaacagttga aacgcgcctt gcggcaaggc
     8821 ggcggcagtt gaagcgcacc gcacggggcg gtagaatcgc aactgcgtcc caatatctga
     8881 cagaaagcac aaaatgaccg atttccgcca agatttcctc aaattctccc tcgcccaaaa
     8941 tgttttgaaa ttcggcgaat ttaccaccaa agccggacgg cggtcgccct atttcttcaa
     9001 tgccggcctc tttaacgacg gcttgtccac gctgcaactg gcaaaatttt acgcacaatc
     9061 catcattgaa agcggcatcc gattcgatat gctgttcggc cccgcctaca aaggcattat
     9121 tttggcggcg gcaaccgcga tgatgctggc ggaaaaaggc gtgaacgtcc cgtttgccta
     9181 caaccgcaaa gaagccaaag accacggcga aggcggcgtg ttggtcggtg cgccgcttaa
     9241 aggccgcgtg ctgattatcg acgacgtgat ttccgccggc acatccgtac gcgaatcgat
     9301 caagctgatt gaagcggagg gcgcaacccc cgccggtgtc gccatcgcgc tcgatcgcat
     9361 ggaaaaaggc acgggtgagt tgagcgcggt tcaggaagtg gaaaaacaat acggtctgcc
     9421 cgtcgccccc atcgccagcc tgaacgattt gtttattctg ttgcaaaaca accccgaatt
     9481 cggacagttc ctcgaacccg tccgagccta ccgtcggcag tacggcgtag aataaaaaca
     9541 aagcatatgc cgtccgaacc gccttacgcc tcagacggca tcaaacctga cacacacgag
     9601 gaaataccat gcccgcctgt ttctgccccc actgcaaaac ccgtctctgg gtcaaagaaa
     9661 cccaactcaa tgtcgcccaa ggcttcgtcg tctgccaaaa atgcgaagga ctgtttaaag
     9721 ccaaagacca tctggcaagc acgaaagaac ccatattcaa cgatttgccc gaggctgttt
     9781 cggatgtcaa actcgttcac cgtatcggca cgcgcgccat cggcaagaaa cagatttccc
     9841 gtgacgaaat cgccggcatc ctcaacggcg gcacaaccca gcccgatatt ccgcccgcaa
     9901 ccgccgccac ccctgctgcc gcaccgcagg ttaccgtacc gcccgccgcg cccgcccgtc
     9961 aggatgggtt caactggacg attgcaaccc tgtttgccct tatcgtcctc attatgcagc
    10021 tttcctacct cgtcatccta tgatcgtgcc cgacctcttt gtcgcccact tccgcgaagc
    10081 cgccccctac atccgccaaa tgcgcggcaa aacgctggtc gccggcatag acgaccgcct
    10141 gctcgaaggt gataccttaa acaagctcgc cgccgacatc gggcttttgt cgcaactggg
    10201 catcaggctc gtcctcatcc acggcgcgcg ccacttcctc gaccgccacg ccgccgcgca
    10261 aggccgcacg ccgcattatt gccggggctt gcgcgttacc gacgaaacct cgctcgaaca
    10321 ggcgcagcag tttgccggca ccgtccgcag ccgttttgaa gccgcattgt gcggcagcgt
    10381 ttccgggttc gcgcgcgcgc cttccgtccc gctcgtatcg ggcaacttcc tgaccgcccg
    10441 tccgataggt gtgattgacg gaaccgatat ggaatacgcg ggcgttatcc gcaaaaccga
    10501 caccgccgcc ctccgtttcc aactcgacgc gggcaatatc gtctggctgc cgccgctcgg
    10561 acattcctac agcggcaaga ccttccatct cgatatgctt caaaccgccg cctccgtcgc
    10621 cgtctcgctt caggccgaaa aactcgttta cctgaccctt tcagacggca tttcccgccc
    10681 cgacggcacg ctcgccgtaa ccctctcggc acaggaagcg caatcgctgg cggaacacgc
    10741 cggcggcgaa acgcgacggc tgatttcgtc cgccgttgcc gcgctcgaag gcggcgtgca
    10801 tcgcgtccaa atcctcaacg gtgccgccga cggcagcctg ctgcaagaac tcttcactcg
    10861 caacggcatc ggcacgtcca ttgccaaaga agccttcgtc tccatccggc aggcgcacag
    10921 cggcgacatc ccgcacattg ccgccctcat ccgcccgctg gaagaacaag gcatcctgct
    10981 gcaccgcagc cgcgaatacc tcgaaaacca catttccgaa ttttccatcc tcgaacacga
    11041 cggcaacctg tacggttgcg ccgccctaaa aacctttacc gaagccgatt gcggcgaaat
    11101 cgcctgcctt gccgtctcgc cgcaggcaca ggacggcggc tacggcgaac gcctgcttgc
    11161 ccacattatc gataaggcgc gcggcatagg cataagcagg ctgttcgcac tgtccacaaa
    11221 taccggcgaa tggtttgccg aacgcggctt tcagacggca tcggaagacg agttgcccga
    11281 aacgcggcgc aaagactacc gcagcaacgg acgaaactcg catattctgg tacgtcgcct
    11341 gcaccgctga ccgcaacgga aagccgccgc agaaatgccg tctgaacccc gtttcagacg
    11401 gcatttcccc gattataaag acctgcccaa ccctcaagga ccccgatgaa atcctacccc
    11461 gacccctacc gccattttga aaacctcgat tccgccgaaa cgcaaaactt cgctgctgaa
    11521 gcgaatgccg aaacgcgcgc gcgtttttta gaaaacgaca aggcgcgcac gctgtcggac
    11581 ggcattttgg cgcagatgca ggacacgcgg cagattccgt tttgtcagga acaccgcgcg
    11641 cggatgtacc atttccatca ggacgcggag tatccgaagg gcgtgtaccg cgtgtgtgcg
    11701 gcggcgacgt atcgttccgg ctatcccgag tggaaaatcc tgttttcagt ggcggatttc
    11761 gacgagctgc tcggcgacga tgtgtatttg ggcggcgtgt cgcacttggt ggaaaagccc
    11821 gaccgtgcgt tgttaacact gagcaaatcg ggcggcgata cggcgtacac gctggaagtg
    11881 gatttggaag caggggagtt ggtagaaggc ggttttcact ttccggcagg caaaaaccat
    11941 gtgtcgtggc gcgatgaaaa cagcgtgtgg gtgtgtccgg cctgggacga acgccagttg
    12001 accgaatcgg gctatccgcg cgaggtgtgg ctggtggaac gcggcaaaag tttcgaggaa
    12061 agcctgccgg tgtaccaaat cggcgaagac gggatgatgg tgaacgcgtg gcgttacctc
    12121 gatccgcagg gttcgccgat tgatttgatt gaagcgtctg acggttttta caccaaaacc
    12181 tatttgcagg tgtcggccga aggcgaggcg aaaccgttaa acctgcctgc tgattgcgat
    12241 gtggtcggct atctggcggg gcatcttttg ctgacgctgc gcaaggactg gcaccgcgcg
    12301 aaccaaagct atccgagcgg cgcgctggta gcggtgaaac tgaatcgcgg cgaaatcggg
    12361 gcggcgcagc ttttgtttgc gcctgatgaa acgcaggcat tggaaagcgt ggaaacgacc
    12421 aagcgttttg tcgtggcgag tctgctggaa aacgtacaag gccgtctgaa agcgtggcgt
    12481 tttaccgacg gcaaatggca ggaagccgaa ctgccgcgcc tgccctcggg cgcgttggaa
    12541 atgaccgacc aaccgtgggg tggcgacgtg gtttaccttg ccgccagcga tttcaccacg
    12601 ccgctgacgc tgtttgcgct ggatttgaac gtgatggaac tgaccgtcat gcgccgtcag
    12661 ccgcagcagt tcgattcaga cggcatcaac gtgcagcagt tttggacgac ttcggccgac
    12721 ggcgagcgta ttccttattt tcacgtcggc aaaaacgccg cccccgacac gccgactttg
    12781 gtttatgcat acggcggttt tggcattccc gagttgccgc attatttggg cagcgtcggc
    12841 aaatattggc tggaagaggg caatgccttt gtgttggcaa acatccgcgg cggcggcgag
    12901 ttcggcccgc gctggcatca ggcggcgcag ggaatcagca aacacaaaag cgtcgacgat
    12961 ttgcttgccg tcgtgcgcga tttgtccgaa cgcggtatca gttcgcccca acacatcggc
    13021 ttgcagggcg gcagcaacgg cggcctgatt accgccgccg ccttcgtgcg cgaaccgcaa
    13081 agcatcggcg cgctggtgtg cgaagtgccg ctgaccgata tgatccgcta tccgctgctc
    13141 tccgccggcg caagctggac ggacgaatac ggcaacccgc agaaatacga agtctgcaaa
    13201 tgccggttgg gcgaattgtc gccgtatcac aatctttcag acggcatcga ttatccgcca
    13261 gcgctcatta ccaccagcct cagcgacgac cgcgtccatc ctgcccacgc gctcaagttc
    13321 tacgccaaac tgcgcgaaac ctccgcgcaa tcttggctct actcacctga cggcggcggc
    13381 cataccggca acggcaccca acgcgaatcc gccgacgaac tcgcctgcgt gttgctgttt
    13441 ttgaaagagt ttttgggcta agggcgggga gcggcactgc cgccgcgaat gaaaaaaggt
    13501 cgtctgaaac tgctttttca gacgaccttt tttaatggt
//
LOCUS       DAWFBO010000040        13383 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFBO010000040 DAWFBO010000000
VERSION     DAWFBO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.040"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(100..528)
                     /locus_tag="WGO87_000843"
     CDS             complement(100..528)
                     /locus_tag="WGO87_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0392225.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLGYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(593..2341)
                     /locus_tag="WGO87_000844"
     CDS             complement(593..2341)
                     /locus_tag="WGO87_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0392226.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSNIKKICETELTVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2668..4101
                     /gene="ccoN"
                     /locus_tag="WGO87_000845"
     CDS             2668..4101
                     /gene="ccoN"
                     /locus_tag="WGO87_000845"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0392227.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4128..4739
                     /gene="ccoO"
                     /locus_tag="WGO87_000846"
     CDS             4128..4739
                     /gene="ccoO"
                     /locus_tag="WGO87_000846"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0392228.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4744..4914
                     /locus_tag="WGO87_000847"
     CDS             4744..4914
                     /locus_tag="WGO87_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0392229.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4938..6035
                     /gene="ccoP"
                     /locus_tag="WGO87_000848"
     CDS             4938..6035
                     /gene="ccoP"
                     /locus_tag="WGO87_000848"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0392230.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6148..6273
                     /locus_tag="WGO87_000849"
     CDS             6148..6273
                     /locus_tag="WGO87_000849"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0392231.1"
                     /translation="MHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            6475..7884
                     /locus_tag="WGO87_000850"
     CDS             6475..7884
                     /locus_tag="WGO87_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0392232.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7986..11195
                     /gene="recC"
                     /locus_tag="WGO87_000851"
     CDS             7986..11195
                     /gene="recC"
                     /locus_tag="WGO87_000851"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0392233.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPHIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11265..12833)
                     /gene="mtrF"
                     /locus_tag="WGO87_000852"
     CDS             complement(11265..12833)
                     /gene="mtrF"
                     /locus_tag="WGO87_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0392234.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFELSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPILAGITQQAAQIIHPDYAVGPEAN
                     WFFMAVSTFVIALIGYFVTEKIVEPQLGLYQSDLSQEEKDFRHSNEITPLEYKGLIWA
                     GAVFVALSALLAWSIVPADGILRHPETGLVAGSPFFKSIVVFIFLLFALPGIVYGQIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LGGSVLFVGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMGYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13167..13379
                     /locus_tag="WGO87_000853"
     CDS             13167..13379
                     /locus_tag="WGO87_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392235.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIARDVSLTTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3350 a         3226 c         3552 g         3255 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgttttc ggcttgtaat cagacgcggg cgttgtccag
      121 ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga tgatttcgtc
      181 aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc gcgtaccggt
      241 ccggcaatag ccaaggacgg gatattcggc ttgtccgatg agttggcgga aggtttcgac
      301 atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag taacgcctgc
      361 ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt cgcggtcggg
      421 gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat cggctttggt
      481 cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt tgtttccttt
      541 actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa gtttaaccga
      601 acaaccagtt gtccaacagg tttcggtctg tgatatgtag gatggcagta tccgctgccg
      661 ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag tggatattga
      721 tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag gcataaccgt
      781 ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg ccgccgttga
      841 agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca aaatcgcctg
      901 ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg ggaagcggca
      961 ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa tcttcggtac
     1021 tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc tgacagcgga
     1081 tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga tagagtttgt
     1141 ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc agatcaaggc
     1201 acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg ctgttttcat
     1261 ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc tgcctcaaac
     1321 ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa taagattcgg
     1381 gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta ataccttcga
     1441 atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg aacgcggcag
     1501 gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt ccgagcaatg
     1561 tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg cggtcggcga
     1621 gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg acgtggagca
     1681 ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgtcagttct gtttcgcaga
     1741 tttttttgat attcgaaacc agcctgttgc ggtcgaaatc gggatatatg ccgcttaagg
     1801 caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag cccatctcga
     1861 caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc cctgtttccg
     1921 gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag cggtgcggca
     1981 gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa ccgcgttctg
     2041 tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag cggatttgcc
     2101 actcgccgcg tacggcggca gcgtgccagc ggttttttgc aatttgttcg agcggcatgg
     2161 acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc cgaatcagat
     2221 agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca tcttgtgtga
     2281 atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta taatgaatca
     2341 taactatcaa atatatcgtt aaacgggata gattaaatac ctttaaaatg cagcttaacc
     2401 tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg cggtcgggtt
     2461 gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc aaagggctcg
     2521 tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa atttagaaaa
     2581 aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga taaaaatctt
     2641 tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta caaagtggtg
     2701 cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt cggcgttatc
     2761 gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc ttggttccac
     2821 ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg cggttgcggc
     2881 ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct tttcggcggt
     2941 tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc cgtcgtcagc
     3001 ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc gatcgatatt
     3061 ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat tgccaaacgt
     3121 aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt ggcggtcgca
     3181 cttttacaca tcgtcaacaa tatcagcatc ccggcagggt tgatgaagtc ataccccgtc
     3241 tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataacgc cgtgggcttc
     3301 ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca agcagcccgc
     3361 cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt tacctatatg
     3421 tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca atctttgggt
     3481 atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa cggcatcatg
     3541 accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt tctgattgta
     3601 tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat taaaacggtc
     3661 aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc
     3721 tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct gttcggcaaa
     3781 gaacagatgc acagcaccaa gctggtagaa gcgcattttt ggattgcgac catcggcgtg
     3841 gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat gtggagttct
     3901 ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg caccatgcct
     3961 tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg cattatggcg
     4021 tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat ccctgcggtt
     4081 tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg aaattacaac
     4141 aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg
     4201 gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag ccggcaccgg
     4261 gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc cgtgagggct
     4321 gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc
     4381 attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt tccaaacgta
     4441 ccggtcctga tttggcacgt gtgggcggcc gctattccga cgaatggcac cgtatccacc
     4501 tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca tggcttgcac
     4561 gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta
     4621 ctccttacag tgatgaggaa attgcgaaag cacctgaggc tttggcaaac aaatccgagc
     4681 tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac
     4741 atcatggata ttaacggtat tcgtgctctc ttcacggtat ggatctttat ctgtttcctg
     4801 ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa tgccgcaaac
     4861 agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt
     4921 gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc tggaatatat
     4981 atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa
     5041 atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat gagtgggacg
     5101 gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt
     5161 ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac aaaggtctgc
     5221 tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat gagcaatacg
     5281 gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa gaccctcaag
     5341 ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac ggctctgatg
     5401 ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg ggcggtgatc
     5461 ccgataaaat tcacgagacc atcgaaaaag gccgtgttgc gactatgcct gcctggggcc
     5521 ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac
     5581 ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc agcggtccgc
     5641 ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt ttgggtccga
     5701 acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc gaaaccatta
     5761 ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa gacaaactgc
     5821 atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct ccggtgaaaa
     5881 aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct gctcctgcag
     5941 aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa
     6001 aagccgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt tgtgccgcct
     6061 gccatggcaa tgcggttccg ggtattcccc atgtaggcat caaagccgat tgggccgacc
     6121 gcatcaaaaa aggcaaggac acgttcgttg cacaaacacg cgatcgaagg tttcaatacg
     6181 atgcccgcaa aaggcggtcg cggcgatttg agcgatgatg aagtgaaagc tgcggttgac
     6241 tatatggtca accagtcagg cggcaaattc taacttgact gagtttccga tattatcggt
     6301 tatgaaaatc cagattcagt attgaatctg gattttttgt tttaacgggc atccggtttt
     6361 aaacgcagac tgcctatttg ttgtaatgaa ttttgacttt agtcataaag aattagtctt
     6421 tattgtttat aatcgcgcat catcacgata gccctatttt caaaaggacg attaatggat
     6481 acacaaatca aaactgaagc cgacaatcag agcaaccggc gttatctgac cgtttggcgg
     6541 tggcattttt atgccggtct gctggttatg ccttttctta ccctgcttgc cgttacgggt
     6601 ttgggtatgc tgctgtttgc caatattacc ggtaaggagg gcgagcggat tcatgttgtg
     6661 ccgcaggcaa cggtacaacc tctgtctgtt caggcggaag cggcacgcag tgccgttaat
     6721 ccggaaactt cgtccgtcgt tcagtatatt gcgccgcgtg ccgatgatat ggttgccgtg
     6781 ttccgtgtca acaatgaggg caaggcaacg atggtcgcgg tcgatcctta tacggcaaaa
     6841 gtggtcagta ccatgccgcg caatcagggt tggtattacg cgatggatga aatccacagc
     6901 gatatgatgc tcggttcgac aggtgattat cttttggaaa cggctgcatc gctgacgatt
     6961 atcatgataa tcagcggttt gtacctttgg tgggtgaaac ggcgcggcat caaggcgatg
     7021 ctgctgccgc caaaaggcag ggcgcgttct tggtggcgga atctgcacgg cgcgtttgga
     7081 acttgggtgt cgttgatttt actgttgttc tgcctgtcgg gtattgcttg ggcaggtatt
     7141 tggggcggca agttcgtgca ggcttggagt cagttcccgg ccggcaaatg gggtgtcgaa
     7201 ccgaaccctg tttcagtcgt gccgacccac ggcgaggtat tgaatgacgg caaggttaag
     7261 gaagtgccgt gggttttgga gcttacgcct atgcctgttt cagggacgac tgtgggcaaa
     7321 gacggtatta accctgacga gccgatgaca ttggaaaccg tcgaccgttt tgcgcgggaa
     7381 atcggtttca aagggcgtta tcagttgaat ttgcccaaag gcgaggacgg agtgtggact
     7441 ttgtcccaag actctatgag ttacgatatg attagtccgt ttgccgaccg cacggtgcat
     7501 atcgaccagt acagcggcaa aatccttgcc gacatccgct ttgacgatta caacccgttc
     7561 ggcaaattta tggcggcaag cattgcgctg catatgggta ctttgggctg gtggagcgtg
     7621 ttggcgaacg ttttgttctg ccttgccgtc atttttatcg gtatcagcgg ctgcgtgatg
     7681 tggtggaaac gccgtccgac cggagcggtg ggcatcgttc cgccggcgca gaaagtcaag
     7741 ctgccggttt ggtggatgat ggcattgccg ctattggcaa tcgcactgct cttcccgacc
     7801 gcactgcttg ccattgccgt gatttggctg ttggatacct tgctgctgtc gcggattcct
     7861 gttttgagga gatggtttaa atgacccgat gccgtctgaa gcgttcagac ggcattttgt
     7921 ttgaaggcgg gcgggggaaa ggctatataa tcccgaatac ttgaccacag cttctgtttg
     7981 aaatcatgtt ttatctgtat caatccaacc gtcttgaaac gctggcggca ctgtttgccc
     8041 gcattcaaaa agtcaaaccg ctgaaatcgg ttttacagcc cgaacagatt attgtgcaga
     8101 gtcaggggat gcgccgctac ctcaatacct gcctcgcccg cgatttgggc gtggcggcga
     8161 atttgtcgtt cagcctgccc gccggtctga cgtggaagct gatgaaaaaa ctgattcccg
     8221 gtattccgga actcagcccg tttgcgcctg aagtgatgcg ctggcggctg ttggatttgt
     8281 tccgcagcga ggcatttcgg aatacggcag aatttgaaga tgtgaggaat gtgctgcaag
     8341 actatctagg cagcggcgaa tcggcagatt atcagcttgc gggacagctt gcggacatat
     8401 tcgaccaata ccttgtctat cgtccgcagt ggatagacgc gtggcagcag ggcaggatac
     8461 tcagtttggg cgacgatgaa atctggcagt ccaaactgtg gcgttatctt gatgacggca
     8521 ggcagagcgc gccgcaccgt gtcgcgttgt gggaaaagct gcttgccgcg ttggacaagg
     8581 ataagctgcc cgagcgttat ttcgtgttcg gcatttccac gatggcgccg atgtatcttc
     8641 agcttttgca caagctgtcc gaacattgcg atgtgttcgt gttcgcactc aatccgagcg
     8701 ggatgtattg gggcaacgtc atcgaagcgg cgcaaatcct caaaggcggc ggcgatcccg
     8761 atttaactca ggcagggcat ccgctgctcg cctcattggg caagcagggg cgcgactttt
     8821 tcgacttttt gaacgaaatg gaaatagaag gagaaacgcc ggtatttgaa gaaggcgggc
     8881 gcgatacgct tttgcacgcc ctgcaaaccg atattcaaaa cctgaaaatg ccgtctgaaa
     8941 atgtgggaag cgtcaacacg ggcgacggct cgatacgcat cgtctccgca cacagcccct
     9001 tgcgcgaatt gcagatactc aaagacaagc tgttgaaaat tctgcatgaa catcccgatt
     9061 ggcagccgca tgatatcgcc gtattaaccc cgcacatcga atcctatacg ccttttatcg
     9121 aagccgtgtt cggacaggcg cagcccggtg cgcaggcatt gccgtattcc gtttcagacg
     9181 tgaaaatcag ccgccgacaa ccgctgtttc atgctttgtc atgcctgttc gacttgttgg
     9241 aaagccgatt tgaagtcgat aaagtgcttg tgcttttaga aaccgcaccc gtgttgcgcc
     9301 gtttcggact gactgaggac gatttgccgc ttttgcacga tatggttgcc gatttgaacg
     9361 ttcactgggg tttggacgga gaaatgcgcg gcggcacgga tcagctgttt acctggaagc
     9421 aggcggtaga acgcatgata ttgggctgga tgctgcccga aggcggcaac ccgatgtggc
     9481 aggatgtcag cgcgtggtat gccgacgtga accaaaccgc cctgttcgga cgttttgccg
     9541 ccctcctcga aaccctgtcg gatattgtac ggatatggcg gcagcccgca acgatcggcg
     9601 aatgggtggc gcgttgccgg gatttgcttg aaacattgtt ccaagctgaa gccgatgacc
     9661 aaaagtcagt ccaaaacctt gaaaacgaat gggtcaaatg gcaggcggaa acagaattgg
     9721 cgcaattttc cggacagttg ccgcaacaaa ccgtcatccg ccatatccga cgtttcctcg
     9781 acagcgaaag cgaggcaggc tttttacgcg gcggcatcac cttttgcagt atggtgccga
     9841 tgcggagcct gccgttcaaa gtcatctgcc tgttgggttt gaacgacgga gattttcccc
     9901 gcaataccaa agccgccgta ttcgacctga ttgccaaaca tcccgccaaa ggcgaccgcg
     9961 cccgccgcga tgacgaccgc tacctcttcc tcgaagccct catcagcgcg cgcgaaatcc
    10021 tctacctgtc ctacatcggg cgcgacatcc gcaaagacga agagcttgct ccgtcttcgc
    10081 tgttgggcga actcatcgac acagttgccg ctatggcggg catcggtagc cgccaacttg
    10141 cacaaaactg gatagaacag catccgctgc aagccttctc gcgccgatat tttcaagaag
    10201 gcggacgttc agacggcata ttcggcacgc gtaccgacta cgccactgcg ctcggacaaa
    10261 cgcccgaacc gccgcaaccc tttttcgacc aacccgtaga aaacgccgaa cctgttgccg
    10321 aaatcggaca ggacgaattt atccgtttct ggcgcaaccc cgtcaaagta tggcttcagc
    10381 agcagcttgc gtggagcgaa ccccatatcg gcgaagcctg ggagcctgcc gaacccttcg
    10441 agccgcaaca cgccgatcaa atcgccgaaa cctacatcga agcacggcgc gaaggacggg
    10501 attttgccca aaccgccgcc cgcatcgggg cggaaagcct cctgccgtcg ggagagttgg
    10561 gcagactttg gcagcagaac ttccaaactg ctgccaaaca aatcgacacg gcggttttaa
    10621 acagccccaa actgccaccg ctttcatatg ccataccgtc ggacgggcaa atcctgaaag
    10681 gcagcttggg caatctgtac cgctgcggac aagtgtttta cgcctacggc aaacccaacg
    10741 cgccgcaacg tatcgctttt ctgctggaac acctgatatt ttgcgccgtt atgccgtctg
    10801 aagccgaaac gcggcaaacc tttatcgtcc aatccggaga aaccgaaata ttggcggaaa
    10861 tcgcgcaaga cagggcattg cagctattgt cggaatggat ggcgtttttc aatatcgggc
    10921 aaaaccgccc gctgccgttt tttgccaaga cctcgcttgc cgctgccgaa gcgtttgccc
    10981 aaaaacaaga ttgggaagcc gccctgaaaa aagcccaaac cgcctattac ggcaacaaag
    11041 tcagcaaagg gcagaaagac tataccgaag tcgccctcgt gttcggcaac gcaagccaaa
    11101 acccactcga acaacccctg tttgaaaacc tcgcccgcct gcttgccgac acgcttgccg
    11161 cagcggaaaa aagggaaggg gccggagcag cctgacggga tggaaaggca aatgccgtct
    11221 gaaaacagca aaaaggtttt cagacggcat tttgtttatc gtgtttaagg tgcgggatag
    11281 aatgtgggcg cgccgggacc gacgggcagt cccaaaacaa atacccaaat gcagaataag
    11341 gcaatccaag caatcaaaaa gaaggcggaa tacggcaaca tcatagaaat cagcgtcccc
    11401 acgcccgcat cttttttgta tttgatcacc gtcgccataa tcagcccgaa ataacccatc
    11461 atcggcgtaa taatattggt aacggaatca ccgatgcggt aagcggcttg aatgacttcg
    11521 ggcgcgtagc cggccaacat cagcataggg acaaaaatcg gtgcggttac cgcccattgc
    11581 gcggaagcgg agcctatcat cagattgata aaggcacaaa ttaaaataaa accgacaaac
    11641 aacacgctac cgcccaggcc gacttctttt aagaacaccg cccctttaac ggcaatatat
    11701 tgcccgatat tcgtccaatt aaaaaatgcg acaaactgtg cggcaaaaaa gatgatgacc
    11761 aaataaagtc ccaaagtact catcgattcg gccatcgcat taacgacttc ccgttcgccg
    11821 cgcaaacttc gggttatctg tccataaaca atgcccggta gcgcaaacaa caagaaaata
    11881 aaaacaacaa tcgatttgaa aaacggcgaa ccggcaacca atcctgtttc aggatgacgc
    11941 aaaatgccgt cggcagggac gatgctccaa gccaataagg cggataaagc aacaaacacc
    12001 gcgcctgccc aaatcaatcc tttatattcc aaaggcgtga tttcattgga atgccgaaag
    12061 tctttttctt cttgtgacaa atctgactga taaaggccca attgcggttc gacgattttt
    12121 tcagtaacaa aataaccgat caaagcaatc acaaacgtac tgaccgccat aaaaaaccaa
    12181 ttggcttcag ggcctacggc gtagtcggga tggatgattt gcgccgcctg ttgggtgatg
    12241 cctgccaaga tcggatcgag cgtgcctaag aacaaattgg ccgaataacc gcccgaaacg
    12301 ccggcgaaag ccgcagccag gccggcgagc ggatggcggc cgagggaatg aaagatgatg
    12361 gcggacaaag ggattaggac gacatagccc aattcagaag cggtattaga taaaatccct
    12421 gtaaaaacaa ccataaaagt agtgagtttg cgtggagatt ttgtgagcaa taagcgcatt
    12481 aatgcggaaa tcaagcccga tttttccgca atccccacgc ccaataaaga aaccaacacc
    12541 gttcccaacg gcgcgaaacc ggtgaaattt ttaacggtat gcgtcaggat tttgatcaaa
    12601 ccgtcagcat cgagcaggct gacaacgtga atcaaaccgt catcggcacg tcctttcgca
    12661 ccaacagggc gcggatcggg gacggatagt tcgaaatacg caccggcggc agaggcaatc
    12721 agcaataaca caatgaaaat aataaaaagc gtaaccgggt gcggcaacat attgcccagc
    12781 cattcgactg tgcgtaaaaa tcgtccgtcc cgttgcgtat cggtttgact catctgcttc
    12841 tccttaaaaa aaatatattg agtgcattaa atttttgaat gtaacatgaa gtgttttgta
    12901 agcaaactaa aataatcgta tatgtatata accgtaatag attaaaacag aaaatgccca
    12961 catcgttatt ctcataaagg cggtaatccg aaaacttgac gttaaagcct tatcaggaat
    13021 gactggaact caaaaaaacg gattcccgcc tgcgcgggaa tgacgggatt ttcggttttt
    13081 gtttgcaaga atgccggaga agaatgcagt ttacattttg aatggtattg ttattaattg
    13141 taaataaata ttggaaggta aatgatatga aaaatgaaat tcaaaaaatc atggacaaat
    13201 ataatccatg gcatgaagat gattttgagt cgtatgaaga cattgccaga gatgtatcgc
    13261 tgacgacaga taaaacgttc attgaacatt atttgttaga agtttattca gaagaaaacg
    13321 gacattttga ccaagagaat gtccatgcaa tgatagaaga aattaaaaat gcaatttagt
    13381 gaa
//
LOCUS       DAWFBO010000041        13232 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFBO010000041 DAWFBO010000000
VERSION     DAWFBO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.041"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            142..309
                     /locus_tag="WGO87_000854"
     CDS             142..309
                     /locus_tag="WGO87_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392236.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYTQNIDFSQW
                     VKSIIKEKIKK"
     gene            582..767
                     /locus_tag="WGO87_000855"
     CDS             582..767
                     /locus_tag="WGO87_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392237.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            968..1168
                     /locus_tag="WGO87_000856"
     CDS             968..1168
                     /locus_tag="WGO87_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392238.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            1300..1908
                     /locus_tag="WGO87_000857"
     CDS             1300..1908
                     /locus_tag="WGO87_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HEZ0392239.1"
                     /translation="MKDNFTMNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSK
                     WIQRRIEEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERND
                     KGRQARRYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIG
                     KLLDMNPGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            1912..2127
                     /locus_tag="WGO87_000858"
     CDS             1912..2127
                     /locus_tag="WGO87_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392240.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(2288..2806)
                     /locus_tag="WGO87_000859"
     CDS             complement(2288..2806)
                     /locus_tag="WGO87_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0392241.1"
                     /translation="MAKTRIKQPAIEAAQDKAEVTAFIRKIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(2831..3319)
                     /locus_tag="WGO87_000860"
     CDS             complement(2831..3319)
                     /locus_tag="WGO87_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392242.1"
                     /translation="MKELPTQLHNAMIDGLTMLLTLRLSGSPAADTVAATAQTWSRVL
                     AHGRAWDEARDVPRFQTAFMVLANEMSRWPSPKDFLDKIPPPPEPLKLEHHYHPTAEE
                     KAKGKSALNRIQGVIKEVLKGKSLIPPPAETATGQILRNRAKVEALAKREREQGLSKP
                     KC"
     gene            complement(3316..3801)
                     /locus_tag="WGO87_000861"
     CDS             complement(3316..3801)
                     /locus_tag="WGO87_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392243.1"
                     /translation="MNVKCPNCGAVHSLDSLINDAEASAVLKAVLEMDVELGKAAIRY
                     IGLFRPAKSRLSWARTAKLLNELLPMIKAQEATRDGVCFHAPAEAWIHGFNETVNARD
                     QGRLKTPLKSHGYLYEILAGRVGQPSAGNQTNQPNRRAALPANPSQTLTAAASLQGLK
                     K"
     gene            complement(3798..3998)
                     /locus_tag="WGO87_000862"
     CDS             complement(3798..3998)
                     /locus_tag="WGO87_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392244.1"
                     /translation="MMFKLIYFGVVFVLGLGMTLVCVANFLKGRCSRKSEILGALVIV
                     FGSVATWLATWLSMMVLSGVIK"
     gene            complement(3995..4162)
                     /locus_tag="WGO87_000863"
     CDS             complement(3995..4162)
                     /locus_tag="WGO87_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392245.1"
                     /translation="MNAKEIAEWLEDRGELMVMKKDGEGFVIAARAPDGIWKTAEAET
                     LAQAITLWEDL"
     gene            complement(4361..4516)
                     /locus_tag="WGO87_000864"
     CDS             complement(4361..4516)
                     /locus_tag="WGO87_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392246.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARVAQKNREREIEEYQARFRRPA
                     EKKEEKK"
     gene            complement(4622..4861)
                     /locus_tag="WGO87_000865"
     CDS             complement(4622..4861)
                     /locus_tag="WGO87_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392247.1"
                     /translation="MYHLFCERKTKWIGLAFWLLFWAVLVGTMLHSCSKPKVSAAKLE
                     MSRRELLADLEAKARVEQYEAMSVEEKMKGIVYER"
     gene            complement(5128..6042)
                     /locus_tag="WGO87_000866"
     CDS             complement(5128..6042)
                     /locus_tag="WGO87_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0392248.1"
                     /translation="MKQINQALQQKLAEFKAKSGMNQTQLARGIGTSPASVSMYLNGT
                     YADKGGNYETIEPKIEAFLEMQDSKARREELVLGFVSTKTTRRIAEVMRDAHEAGEIA
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLAAAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSIPLNLGDSMPDEELFEIAKAALPDADKETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKIQAGIVKKYSSLIIR"
     gene            6088..6345
                     /locus_tag="WGO87_000867"
     CDS             6088..6345
                     /locus_tag="WGO87_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0392249.1"
                     /translation="MKEFPDLEKRLIMLEVAVQDLEDRSLADSLVLAWLLQRITRQEP
                     TSIGQVRRFLQAQAKTFEPDSVQREHLESLLEIVESAQEPA"
     gene            complement(6412..8388)
                     /locus_tag="WGO87_000868"
     CDS             complement(6412..8388)
                     /locus_tag="WGO87_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0392250.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRTAKVG
                     ERTLDGWISAYLKAEDATERLVALAPKTTKTVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHYGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFAGSGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAPDMLSA
                     EELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVNVFDMD
                     GKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNVIEAEYAVLPKTGTDDGIFLFEADM"
     gene            complement(8390..8641)
                     /locus_tag="WGO87_000869"
     CDS             complement(8390..8641)
                     /locus_tag="WGO87_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0392251.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            8689..9588
                     /locus_tag="WGO87_000870"
     CDS             8689..9588
                     /locus_tag="WGO87_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ0392252.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCDVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            9750..9962
                     /locus_tag="WGO87_000871"
     CDS             9750..9962
                     /locus_tag="WGO87_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0392253.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            10184..10762
                     /locus_tag="WGO87_000872"
     CDS             10184..10762
                     /locus_tag="WGO87_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ0392254.1"
                     /translation="MFRHNGEVLLDGINIKLKSGDSLLIRGPSGCGKTSLLRALAGLW
                     PFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELAEAMNTCRLGYL
                     IDKLDKTDDWQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPTEALLYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMHLDVGNVACG"
     gene            10867..11535
                     /gene="hda"
                     /locus_tag="WGO87_000873"
     CDS             10867..11535
                     /gene="hda"
                     /locus_tag="WGO87_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0392255.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            11532..12200
                     /locus_tag="WGO87_000874"
     CDS             11532..12200
                     /locus_tag="WGO87_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0392256.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            12556..12663
                     /locus_tag="WGO87_000875"
                     /pseudo
     CDS             12556..12663
                     /locus_tag="WGO87_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         3458 a         3572 c         2781 g         3421 t
ORIGIN      
        1 ctattcaaat tctcatcttg gtcgcagttt tcatgttggc aagtgctaat gcctactaaa
       61 gactgaccag ctgggggcgg tcgccgctgt ccccagtccc aatcttacaa aatacggttt
      121 aaaaaatcaa ggagtttgcc tatggttgat gaaaaactgg cagaaaaccg aaagcgttac
      181 gagcagaaac gggtgattaa gaaagtctcg ttcaatgccg aaacggaaaa agaacttttg
      241 gaatataccc aaaatatcga tttttcccaa tgggttaaat caatcatcaa agaaaaaatc
      301 aaaaaatagc ttgactaaat tctagaaata ctagataata cgcctatctg ataaagcaaa
      361 gccccgaagc ggcaacttcg ggggcttatc ggatagaaag gaggcttgtg gaaatgacaa
      421 aactggcgaa agttgttttt ttcatcattc tgcttttaag ctgtagtccg gcttactaag
      481 caagttgatt aaacacaagg ggtggcagaa caccgcgccc ctttcgccaa cctttctgca
      541 agccatttta ctacaacgat tttaaaaatc aaggattgcc tatgcctgat gaaaaaacgc
      601 taaaagcaaa acaagctgaa tatcagcgaa agtatgacca aaagcggctg atgaaaacag
      661 tttctttcca tttggaaaga gagaaagagt tggtggattt tatgcaaaaa gaaattcccg
      721 acttttcaaa ctgggttaaa tcagtcatga aggaaaaaat gaaataagaa aattccgccg
      781 atgggaaaac atcggcggaa caggtggaaa gcagagcagc aactctcttt atgtagggca
      841 tgaaaaaagg ccgtctgaaa caggttttaa accccatttc agacggcttt ttgttgtaat
      901 acatcttgat taaaatggtt tttagtatta ccataatcgt gttttaaaac ctatttaaag
      961 gaagattatg gactcgatta aaaaggctca agaaaaatac gcactcaagc gagtagtgaa
     1021 aacagtatcg ttcaattccg aaaaagaact tgatttaatt aaattttctg aaaatttaga
     1081 tttttcaaac tgggtaaaat cgaaaattag aggagatatg gaatcggaaa aattcaaaaa
     1141 aaaagcaaaa aagaatattg acaaatagaa ttaccataat ttaaaatttc catatcttgt
     1201 gcaacacaag atatggaaat ccccgataag tgacagctta tcggggatag tggaaaagca
     1261 acgttagagc gttcttttcc ttgtcatctc aaccttgaaa tgaaagataa ttttaccatg
     1321 aatacccaac tcatcccaac tgtttcaggt caattggaca atcaaacaca agcactggtt
     1381 gatgctcatg acctgcataa gttcttaggt gtggaaacac cattttctaa atggattcag
     1441 cgtcgcattg aagaatacgg atttacacaa gcccttgatt ttatcggcgt ggacaaaatt
     1501 gtccgcaccg aagcaggttt ctttggacag cgtgataaaa ctgttcaggg gtattattta
     1561 tccctcgaca tggcaaaaga gctgtgcatg gtggagcgca acgacaaagg acgccaagcc
     1621 cgccgatatt ttatcgaaat ggagaaacag gcaaaagccc tgcctgatgc cgtgctatac
     1681 cgcatcgatg cattggaaga cgcctatttc caagccgcgc ccgaaatgct cgcgctgctg
     1741 cgctaccgca gtatgggctt gaacctgacc gaaataggca aactcttgga catgaacccc
     1801 ggcgcggttt cctaccgtct caaaaaactc aacgatttgg gtttcttgga atatgtcccc
     1861 aagcccaaaa tggtggtgca acaacagtct ttgggattgg agggttaagc catgaatacc
     1921 acctataccc tgactttcga ccaagacagc ctcaaagccc tgaatctgct ggtgttcaca
     1981 ctcaaccatc tgaaagtttt ggagatggac atggaaggaa tcgaagacgg tttgtctgcc
     2041 gtgattgaaa ccgcctccga gaaagccgac aagctgtccg ccgctttcta caacgccgtg
     2101 tacgagcagg aagagaaagc agcctgaatc tagggcatga aaaaaggccg tctgaaacag
     2161 gttttaaacc ccgtttcaga cgacctttaa atcatgggtt aaagcctatt tgaaccggct
     2221 aggcaagctc ctgctccgta ggctcaatca cgtttcaaca cactgaattg aaatcaggct
     2281 gttgatttta tagaatttgt tcgctcggct caatcacaaa atcctccacg ccgctcacaa
     2341 tcttaatccc cggcacttgg ccgtctgaaa aacgctcttt ttgattcagg atggcgtctt
     2401 tgtcgatttc ctttttagtg cggacaaact cggcaaaggc ggatttttcc gagagccacg
     2461 ccaagacggc ggccacgccc gttaccttga cggatggcgg acggatgcgc catttaatca
     2521 ggccggtggt aaagtccacg gttttggttt taccgttttc cgtcagctcg tccttatgtg
     2581 cctcgcagta tgcggccaca cgttcggtca ggctcatgat ttcggcacac atcggcgcgg
     2641 ctttggcggc atattcttct tcgatggccg cttttttgtc tccggcttcg gtttccaggc
     2701 gtttgacttc gcgttgcaaa tcgccgattt tgcggataaa cgcagtaact tccgctttgt
     2761 cttgtgccgc ttcgatagcg ggttgtttga ttcgggtttt agccattttc tctttccttt
     2821 caggttgggt ttaacatttc ggcttgctca agccttgttc gcgttcgcgc ttggcaagtg
     2881 cctcaacttt cgcgcggttt ctcaaaatct gcccggtggc ggtttcagcc ggaggcggta
     2941 tcagcgactt gccttttaat acctctttaa ttacgccctg tatgcggttt aaagccgatt
     3001 tccccttcgc tttttcctct gccgtagggt ggtagtggtg ttccagcttc aacggctccg
     3061 gtggcggcgg aatcttgtct aaaaaatctt tcggactcgg ccagcggctc atttcattgg
     3121 ccaataccat aaaggccgtc tgaaagcgcg gtacatctcg cgcttcgtcc cacgcccggc
     3181 cgtgcgccaa tacacggctc catgtttgcg cagtggcggc cacagtgtcg gcagccggcg
     3241 aaccgctcag acgcagggtc aaaagcatgg tcaggccgtc aatcatggcg ttatgcagtt
     3301 gggtaggcag ttctttcatt ttttcagtcc ttgcagcgag gcggctgcgg tcagggtttg
     3361 gctgggattg gccggcaggg cggcgcggcg gttgggttgg tttgtctgat tccctgcgct
     3421 tggctggccg acccggcctg caaggatttc atacagatag ccgtgcgact tcagcggcgt
     3481 tttcagacgg ccttggtcgc gtgcgttaac ggtttcgtta aatccgtgaa tccaagcctc
     3541 ggcaggggca tggaaacaaa ccccgtcgcg tgtcgcctcc tgcgccttaa tcatcggcag
     3601 caactcattc agcagtttcg cggtacgcgc ccaagagagc cgggacttgg cagggcggaa
     3661 taaaccgata taccgtatcg ccgccttgcc caattccaca tccatctcca acacagcctt
     3721 taataccgcc gaagcctccg catcattgat taagctgtcc aggctatgca ccgcaccaca
     3781 gttcgggcat ttcacgttca tttgattact ccggacaaca ccatcatcga caaccatgtg
     3841 gccaaccatg tggccaccga accgaaaaca atcaccagtg cgcccaatat ctccgatttc
     3901 cgcgaacagc gacccttgag gaagttcgcc acacaaacca aagtcatacc aagacccaac
     3961 acaaacacca caccgaaata aatcaactta aacatcacaa atcctcccat aaagttatcg
     4021 cctgagccaa agtttccgcc tccgccgttt tccagattcc atccggcgca cgcgcggcaa
     4081 tcacaaagcc ttcgccgtct tttttcatga ccatcagctc gccacggtct tcgagccatt
     4141 ctgcaatttc ttttgcattc attttcaaat tccttttaaa tcaatatctt atattttcaa
     4201 caaggcaaaa aaatatagag caacatcaac gtcttaccgt tttaattgtc gtcatacccg
     4261 tcatggcctt caccgatcat gtgcaacacg aggattcggg ccggcagttc aagccaaatc
     4321 cccagcacca caaacaccgc caatccgaca acaaaccaaa tcattttttc tcctccttct
     4381 tctcggcagg ccgtctgaaa cgcgcctgat attcctcgat ttcgcgctct ctgttttttt
     4441 gcgccactcg cgccgtcgca cgcctgcggt gttgtcccca agcctgccaa tccgtattgc
     4501 gtcgtccgaa actcatttca cacctccttt cacaatcgcc ttatcgccat atttcgcgcg
     4561 gatttccttt accgcccgat ccaaagcctc ttgtttcgcc gcagggttca ataaaatcgg
     4621 cttatcgctc ataaacaatc cctttcattt tttcctcgac gctcatcgcc tcgtattgct
     4681 cgacccgggc ttttgcctcc aaatccgcca gaagctcgcg gcgcgacatt tccaactttg
     4741 ccgccgacac cttgggctta gagcagctgt gcagcatcgt gcctaccaaa accgcccaaa
     4801 acaacaacca aaaagccaaa ccgatccact tggtttttcg ttcgcaaaac aaatgataca
     4861 ttttcctatt tccttataaa tcaattactt aatattttct taaggcaaaa aaattattgc
     4921 gcacccaatc cgccctaacc ggcgcgcccc cttcgctccc tctccctgtg ggagagggct
     4981 gggggagagg gcaactcctt ctttgcgctt gccgcgtaca tcggcccgcc aaattttccg
     5041 ccgtttatgg gtttcataat cgtgccaagt gtcctcatag actccggcgt gtaccgcata
     5101 ttcgtgtctc atttcagacg gcctttctta tcggataatc agggagctgt atttcttaac
     5161 gataccggcc tgtatcttga tgccgttctt gttcgccgtg cgtaccgcgc cgcgcatcaa
     5221 tttgctcatc cggcgcgtat tgccgttact gtgtttcacc agttccaaga gcgtctcctt
     5281 gtccgcatca ggcaaagccg ctttcgcaat ctcaaagagt tcctcgtccg gcatcgaatc
     5341 tcccaaattc agcggaatcg acacgcggct gtaaagctgt accagctcgc catgcttacc
     5401 ccgcaaattc gccaccagtc ggggcatacc gctcaacacc aaaccgcagc ccgtctcgtc
     5461 gtgcagtcgg cggataatct caagggcgcg caagggcagg ttttccgctt catcgaccac
     5521 aatcagacgg cccgaatcgc gcaggcggtc agataccgat tcaaacaaat cattcaggct
     5581 gcccatcgcc gataccttcg ccgctgctgc cagcttgcgc atcagcacaa gtgccgtaaa
     5641 gctcggattg gcctcaatca ggatggcggc agggtttttc tcgcagtagt ttttgaccgc
     5701 ctgcgtcttg cccaagcctg cctgaccgta aatgacggca atctcgccag cctcatgcgc
     5761 atcgcgcatc acttccgcaa tacggcgtgt cgtcttggtc gatacaaacc ccaacaccaa
     5821 ctcttcgcgt cgcgccttac tgtcctgcat ctccaaaaac gcctcgattt tcggctcgat
     5881 ggtttcataa ttgccgcctt tatcggcata agtgccattc agatacatac tgacggatgc
     5941 cggcgaagta ccgataccgc gtgccagttg ggtttggttc atgcctgatt tggctttaaa
     6001 ttcagccagt ttttgttgca atgcttgatt gatttgtttc atttttaata tccttgagtt
     6061 ttaaacaacc tttaaaggcc gtctgaaatg aaagagtttc ccgatttgga aaaacgcctg
     6121 attatgctgg aagtcgccgt tcaagacttg gaagacaggt cgcttgccga ttccctcgta
     6181 cttgcctggc tgcttcagcg gattacccgt caagagccga cttcgattgg gcaggttcgc
     6241 cgcttccttc aagcgcaggc aaaaacgttt gagcctgatt ccgtccagcg ggaacacctt
     6301 gaatctttgc ttgaaatcgt tgaatccgcc caagagcccg cttgagtttc aatttttcaa
     6361 caagctcggc cgaaaacgcc gcatttttct gtttttccat catgttttcc tttacatatc
     6421 cgcctcaaac aagaaaatcc cgtcgtccgt acccgttttc ggcaataccg catactccgc
     6481 ctcgatgacg tttccgccca aatgtcccag ctcgtcccaa accgcagcct gttccaaagc
     6541 cggattgact tccgcattcg cgagcttgat tgcgttttcc gcccgcttga ttttgccttt
     6601 tcggcgtttt tccgccagtt ggtcgatacg tgctgtcggg aaagcctcgc gtgtattgcc
     6661 gttggcctgc gccttagtga taaacttgcc gtccatatca aacacattga ccgccgacgc
     6721 atcgctcaaa tcgtagctga cccgtacctc gtctttgtga tactccgcca gctcggttga
     6781 gaaataagag ttgttgaata aatccagcca accgcgctgt acctttcgca cctcttgcgg
     6841 cataaacatc gtcgccagct cttccgccga caacatatcc ggcgcaatcc cgtcctgttc
     6901 cagcctcatt tcccgataag ccttcggcga ataatgcccg ccgtccggat gtcggggcag
     6961 ctcgccgtgc gggcggttgt tgtattcatc gatacacttg accacatccg cgataaaacg
     7021 cgaccagctc ggcagttttt tcaaatattt ctgttgttcc tccgtcaaat ccttgccttt
     7081 ttccaaagca ttaaaagcac tttccatctt gcggtacatc aggttcttcg tgctgctgtc
     7141 catcccgctg cccgcaaacg tctcatactg ccgcgccatc tcaatcagat tgtctttcca
     7201 ccatcgctcg atgatgccgc gcccttgcgg gttgcccgcg atacccgttt catgccggat
     7261 acccagtcgg gacgtgatac ccgtgatttc atggtctatc gtcttgccgg tttgaccgcc
     7321 gccgttatcc gagtaataaa taatcggcaa accatagtgc ttgaccccga tacgcagagc
     7381 gtccgacact gctacgcaac tttcagccag cgacaccgaa aatcccacca caaaccgcgt
     7441 acatccgtcg ataatcaccg tcacttccgg cttaaacggc ctgccgtgta ccggatgcgc
     7501 caccttcgcc ttaaagctat ggccgtcgcc gatccaaaca tcgttcggct ttaaagcccc
     7561 ccaatcacgt ttcacataag gcagcagcga tttataagcc gcccccgttt tcctgccgcg
     7621 ctcctgcata atcagcggca gcttgtccca aacgcgccgc accatactca aattaggcac
     7681 atcattgacc ggcatatttt ccgcttccgc ccactgcaca aaccggcggt agctgtgtgc
     7741 cagtttcggc gcggacggga tattgtgaaa ctgcataaac atcggcaacc aaccgtagct
     7801 ctcaatcggc ttgactgtct tcgtcgtctt cggagccaaa gcaaccaacc gctccgtcgc
     7861 gtcttccgct ttcaaataag cagaaatcca gccgtctaaa gtacgttcgc caacctttgc
     7921 cgtccggctg cggtcattgg ccttttccaa gttcccaagc gtgaccgcgt ccaatttacc
     7981 ttctgccagc aagcccaaaa actgagccac cgcagccttg gcagagcaat cgtattggta
     8041 tttaatcccc aacaccgccg ccaccaccgc acatcgcgca tccgccaccg accgttgttt
     8101 ctcgttcaac agcttggccg cttcagccag tgcttgagcc gacatcgccg tccccggtct
     8161 gacttggggc agggttttcg gcatcttctc cgccagctcg tccgactgcc gtttcatgat
     8221 ggctgctcgg atttcggcag ggagggcata ttcaacacca ccacccttac cttgacgcct
     8281 tctcgtttgc caaccatccc tttgcgcttt ttttctcagc cctattactg ttcttggcat
     8341 atttggcaac ccaaaattaa gcaggctttt gagatccata tattccattt caacccactc
     8401 cgagcatctc tttcaattca gctatctttt tgtctcgaca atctgttttc ttgtaatgcg
     8461 gaaaaacttc aactaaatct ttttgaatca ctttcgcgat tgcttccgca attcttttgc
     8521 ttcctcgtcc atttcttatg acctcagaaa cagattggtt agttacccct aaagccgaag
     8581 ccaacattct tgccgatata tttttttctt ctaaggattt atagatttcg ttcgcttgca
     8641 taatgcctcc atgatagaat ccattactta cttaccttac aggctttaat gcctgcctta
     8701 actgttactt ggatgatata cacaaaatat ttggtatacc aaatatattt ggtaatattt
     8761 tgcgatgttt ttatggaacg cattgattta gcattgaatt tagttgaaga gagaagccga
     8821 cttggatact ctcgaaagaa ttttgctgag caaactggat gcagtgcaga aagtttgaga
     8881 ctgtatgaaa gcgggcaggt caacattcct gcggactttt tacttgctgc tgtccaatta
     8941 ggggtggata ttcaatttgt ctttacaggc attcattcgg caaatttaaa tgctgtttca
     9001 gacgacctta ataaaaaatt ggacgacgct attggcaaat caaacgttga gaatcagatt
     9061 aacggcaacg tcagtaacgc tgttatcgcc tcatctggtt cggttgttca tcaaattaat
     9121 acccaaaatc acgtcatcag aacccgtgtc gattcaaaac ccggcaagga acatataacg
     9181 cttgagcaag cttcaaagct gcaacaactt gttaaacaag ttgccgcagc agaagagatt
     9241 gccaaacggt cgccaaaatc aatccgtgca atttgggcta gtctgaatgc tcactgtaaa
     9301 gttccaagtt acaaactaat cgcattgagc gattacgaca aagcagaaac ttatctcaga
     9361 aaatggctgg gtaggttgag caatacagca acatcaaaga acaacgatcc cgactggcga
     9421 aaaaagaaat acgcttatat caaattaaat gtaaaacagc ttgagcttga agattggttg
     9481 aaaagttacc tcgaaaaaaa tttctcagta gaaagcctga ctgaattaag tgatgaagat
     9541 ttgcaaaaaa catatgcagc agtatcgact aaaaaacgga aaaaataggc ttaaacatga
     9601 aactccgcct ttaaattcaa gtttcatgtt tagcttatgg tcttcagatg cttttttaga
     9661 tgtatgatat taaaagattt tttcaaacat ggaactatta tacaagttcc atgttcagtt
     9721 tctagttaaa gccttttata aacattctaa tcaaccacgc cgaaaccgag ccgcgcgacg
     9781 gcctatatgt cttgcgtata ggcaatgacc tctttgtcaa acgtgtgcaa cgtataccgg
     9841 gcaaactgct tgtaacgtct gaaaatccac gttacgcacc gtttgaaata gatttgagta
     9901 atacccaaga cgacatcgcc atcataggcc gtgttgagtg gtatggccgg agtattgatt
     9961 aaatccattt taaaaacctc ttaaaacagt tttaaaaatt ccctaatctc gtcagcttta
    10021 aacagaaaac cgcgcattcc ggcgcggttt tgtgaaaaag ctggcgtaac ttttccggat
    10081 acaaaaaaac gccgaaatcc acgtcttccg aagatttcag cgttttttta ctctatttgt
    10141 tccttgtgca aaaactaaca gtcccccaca ggaaaacgtc gccctgttcc gccacaacgg
    10201 cgaagtttta ttggacggca tcaacatcaa actcaaaagc ggcgattcgc tgctgatacg
    10261 cggcccgagc ggttgcggca aaacctcgct gctgcgcgcg ctggctgggc tttggccgtt
    10321 cggcagcagc ggcaaagtca gccgtccgcc gcatcaagac atcctcttcc tgccgcaacg
    10381 cccgtacacg gcacagggca gcctgcgcga cgcggtttgt tatccggaca tcgacaaaca
    10441 gcatcccgag ttagccgaag ccatgaacac ctgccgcttg ggttatctga ttgacaaatt
    10501 ggacaaaaca gacgactggc agcacaaact ctccccgggc gaactgcaac gcgtcgcctt
    10561 cgtccgcgcc ctgctttcca agcccaaaat cgtcctgctc gacgaagcca ccgccgcttt
    10621 ggacgaaccg accgaggccc tgctctaccg cgcactgaaa caaaaactgc ccgacagcat
    10681 catcatcagc atcggccacc gcagcacgct caacgcgttc cacaatatgc acctcgatgt
    10741 cggcaacgtt gcctgcggtt gaacgatgcc gtctgaagcc gttttcagac ggcatttttc
    10801 aaattgataa agcacgcgaa aacaacgtac aataaacccg tttaccaccc ctcggaatgc
    10861 cgaaccgtga accagcttat tttcgacttt gccgcacacg actatccgag tttcgacaaa
    10921 ttcctcggca cggaaaacgc ggaactggtc tatgtcctcc gacacaaaca cggacagttt
    10981 atctatgtct ggggtgaaga aggtgcgggc aaaagccatc ttttgcaggc gtgggtcgca
    11041 caggcgctcg aagccggcaa aaacgccgcc tatatcgatg ccgcctccat gccgctgacc
    11101 gatgccgcgt tcgaggcgga atacctcgcc gtcgatcaag tcgaaaagct gggcaacgaa
    11161 gaacaagccc tgcttttttc catcttcaac cgcttccgca acagcggcaa aggctttttg
    11221 ctgctcggtt cggaatacac gccccagcag ctcgtcatcc gcgaagattt gcggacgcgt
    11281 atggcgtact gcctcgttta cgaagtcaag cccttaaccg accaagaaaa aatcgacgcg
    11341 ctcgccagta tggcggcggc acgccaagta accgtcgatt ccgaaatttt cgaatacctg
    11401 ctcaaacact ggcggcgcga tatggacagc ctgatgatga tgctcgacac gctggacaac
    11461 tacgccgtca caatgggcaa acgcatcacc ctgccgcttt tgcgccagct tttgaaacaa
    11521 caggaaaccc aatgaaaaac ctcgccatct tcgacctcga caacaccctc atcaacaccg
    11581 attccgacca cgcctggccg caatacctca tcaaaaaagg cttggttgac gctgccgaaa
    11641 ccgaagcgca aaacgaaaaa ttctaccgcg actaccaaaa cggctgcctc gacatcgacg
    11701 cattcctcaa attccacctc gccccgctcg ctcgttacag caaagaagag ctggcggaat
    11761 ttcaccgcga atttatggcg gaatacatca tcccccacat ctcgcctatg cagcgtatgc
    11821 tggtgcagag ccaccaaatg gcaggcgacg aaacccttgt gatttcctca accaacgagt
    11881 tcatcatcac ccccgtctgc caccttttcg gcatcaccaa catcatcggc acacaactcg
    11941 aaaccggttc tgacggccgc tacaccggca attacatcgg cacgcccagc ctcaaagaag
    12001 gcaaaatcac ccgcctgaac caatggcttg ccgaacgcgg cgaaacgctc caaagctacg
    12061 gcaaaaccta tttttacagc gactccaaaa acgacttgcc gctgctgcgc cttgtcagcg
    12121 aacccgtcgc cgtcaacccc gatgccgagc tggaaaaaga agccaaagaa aaaggctggc
    12181 cggttttgaa tttcaaatga cgcaatgccg tctgaagccc gattcggcgt tcagacggca
    12241 ttgaggctga aatccaaaaa actcaaagcc acaggaattt atccggaaaa acaaaaaaca
    12301 caggaattca tcgggaaaaa caacaatctt tccaccgtca ttcccacgaa agtgggaatc
    12361 ttgatccgtc cgcacggaaa cttatatttc gtcatttgca cccgacataa ttcaatactt
    12421 tattttgcaa taaattaacg gatgctgaca aattagattg gattaattac cctattatcc
    12481 atattgggtt atcgggttgc taattgagca gagcgtagca aaccataaag tgagtgtttc
    12541 cacccactct gcatttatag tggattaaat ttaaatcagg acaaggcgac aaagccgcag
    12601 acagtacaga tagtacggca aggcgaggca acgctgtact ggtttaaatt taatccacta
    12661 tactatgaag tggttattaa atatgataat gagacctatt aaatttagta tggtaaatac
    12721 gttattattt attgttatat gtagttcatt ttttgatctg ctcgttcaat tatgtacaat
    12781 tttatttcat agccaaaaaa tatactttat tacattattt ttattattta tttttaattt
    12841 tgttacaaaa tctatctata tggcaattat ttatcctatt ttatattttt ttacgataaa
    12901 aaaatattat ccttactcta ggaaagtgat aattctatta ttattagcat tatctatata
    12961 ttttagtttt atggactttt actttttttc catatattca gataacctta gctatgaaac
    13021 ggagccttta catttataca tccctattat tattaatttt ttctcacttt tagtttctaa
    13081 ttttatttta tcttttatca acaagtaaat gtatatacaa aatacaatcc aaaagatcct
    13141 aagcaaacca ccaatcccac accaccctta ccggttcaaa tccgcacaaa aacccacatc
    13201 ccgtcattcc cacgaaagtg ggaatctagg ac
//
LOCUS       DAWFBO010000042        12735 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFBO010000042 DAWFBO010000000
VERSION     DAWFBO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.042"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            332..1645
                     /locus_tag="WGO87_000876"
     CDS             332..1645
                     /locus_tag="WGO87_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0392257.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1863..2038
                     /locus_tag="WGO87_000877"
                     /pseudo
     CDS             1863..2038
                     /locus_tag="WGO87_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2034..3284
                     /locus_tag="WGO87_000878"
     CDS             2034..3284
                     /locus_tag="WGO87_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0392258.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3319..4812)
                     /gene="rng"
                     /locus_tag="WGO87_000879"
     CDS             complement(3319..4812)
                     /gene="rng"
                     /locus_tag="WGO87_000879"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0392259.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            5026..5283
                     /gene="grxC"
                     /locus_tag="WGO87_000880"
     CDS             5026..5283
                     /gene="grxC"
                     /locus_tag="WGO87_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0392260.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5304..5747
                     /gene="secB"
                     /locus_tag="WGO87_000881"
     CDS             5304..5747
                     /gene="secB"
                     /locus_tag="WGO87_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0392261.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5833..7875
                     /gene="recG"
                     /locus_tag="WGO87_000882"
     CDS             5833..7875
                     /gene="recG"
                     /locus_tag="WGO87_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0392262.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTATETLEQLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLSELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFANLEEDLHLLEQAREIAPLLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(8630..9673)
                     /locus_tag="WGO87_000883"
     CDS             complement(8630..9673)
                     /locus_tag="WGO87_000883"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0392263.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIWDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGGIPLLP"
     gene            complement(9670..9951)
                     /locus_tag="WGO87_000884"
                     /pseudo
     CDS             complement(9670..9951)
                     /locus_tag="WGO87_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10204..11358
                     /locus_tag="WGO87_000885"
     CDS             10204..11358
                     /locus_tag="WGO87_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0392264.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTATQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            11587..11940
                     /locus_tag="WGO87_000886"
     CDS             11587..11940
                     /locus_tag="WGO87_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392265.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            12001..12627
                     /locus_tag="WGO87_000887"
     CDS             12001..12627
                     /locus_tag="WGO87_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ0392266.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
BASE COUNT         2947 a         3129 c         3588 g         3071 t
ORIGIN      
        1 ccgccttgtc ctgatttttg ttaatccact ataaaatccc acgtgtacgg cacgccccgc
       61 gctccgcaat gataggggcg gtttcgtttg aatcggcgta ccgggatgaa tgcccccgcg
      121 ccgtcccgat accggtttgc cggttgttcc gtatcggttg ccgccgtgaa atgacggacg
      181 gatacaggaa tggcggcagg cgtaaatggg aggaaatgac cgattgactt gtttcaaaat
      241 aaaaaagtag aatacaaggc ttatcccgcg tctgtgggcg gttttgcctg cacgggcggt
      301 tttctgttta aaataaaata aaggagaaat gatgctgacg tttatcggac tgctgattat
      361 cggggtcatc gtatggttgc tgctgacgga aaaagtgtcg cccatcatcg cattaatctt
      421 ggtgccgctg tttggggctt tgctggcggg gtttgatgta tcccaattaa aagaatttta
      481 ttcgggcggc accaaatcgg tgatgcagat tgtgattatg tttatgtttt ccattttgtt
      541 ttttggaatc atgaacgatg tggggctgtt ccgtccgatg ataggcggtt tgattaagct
      601 gactcggggt aatatcgtgg cagtgagtgt ggggacggtc ttggtgtcgg tggtggcgca
      661 gttggacggg gcgggtgcga cgacgttttt attggtggtc cccgcccttt tgccgcttta
      721 caagcgtctg catatgaatc cttacctgct gtttttgctg ctgacttcca gtgcgggatt
      781 gattaacctt ctgccgtggg gcgggccgac tgggcgggtt gcaagcgtgt tgggcgcaga
      841 tgtgggcgaa ttgtataaac ctttgttgac ggtgcaaatt atcggtgtgg tgtttatcct
      901 tgcgctgtcc ctgcttttgg gtgtgcgtga aaaaaggcgg attgtccggg agttgggcgc
      961 gttgcccgcc gtggcggatt tgataaagcc ggtgcctttg tcggaagaag aacaaaaatt
     1021 ggcgcgtccg aaactgtttt ggtggaatgt cctgctgttt ttggcggcga tgagcctgct
     1081 tttttcgggc atcttcccgc cgggttatgt atttatgctg gctgcaacgg cggcgttgct
     1141 tttgaattac cgcagcccgc aggaacagat ggagcggatt tatgcccacg ccggcggcgc
     1201 ggtgatgatg gcgtccatta ttttggcggc aggtacgttt ttggggattt tgaagggtgc
     1261 ggggatgttg gacgcgattt ccaaagacat tgtgcatatc ctgccggacg cgctgctgcc
     1321 ttatctgcat attgccatcg gtgtgttggg cattccgctt gagttggttt tgagtacgga
     1381 cgcttattat ttcggactgt ttccgattgt ggagcagatt acctcgcagg cgggcgtggc
     1441 gcccgaagca gcaggttatg cgatgttgat cggcagtatc gtcggcactt ttgttacgcc
     1501 gctttcgccg gctttgtgga tgggcttggg tttggcgaaa ttgtcgatgg gcaaacacat
     1561 ccgttattcg tttttctggg cgtggggttt gtcgctggcg atattggcca gttcgatagc
     1621 ggcaggaatc gtgcctctgc cgtaaacggc gaagccgtct gaagcccgaa tcgttcctgt
     1681 ggtctgtcgc aggctctgcc tgcccgtgtg ttgcaatcgc gccccaatgc cgtctgaagg
     1741 cagttgccgg cgcggttttt tgtgccgcag ttttgaaaaa taccggcaag cgtaaaggcc
     1801 gctaaattgc ggctaatttt ggggtgttag aataggcacg gtttattttg aagggaaagt
     1861 tgatgcgtgt tttgctggtg gaagacgatg cgatgattgc ggaggcggtg tcggcaagtt
     1921 tgaaagacgg cggctatgcg gtggattggg tcaaaaacgg cgcgcggctg ccgctgccca
     1981 gccttatgac ttgatgctgc tggatttggg tttgctgggg cgggacggtt tggatgtttg
     2041 ttccgctggc aatgcttgca ggtatgtttt cctactacga aaccttccac gaaacggaag
     2101 cgttgcagga cgacctgctc cgtcaggcgg cattgtacgt cgcacccgat tccaaacccg
     2161 aaactttgcc cgagggcgac ggcgatacgc gcattttcgt gcagatgccg cagcaggaag
     2221 accctgttgt cagcctgcct gcgcatctgg cggacggtct gcacacgctt caggcggacg
     2281 gggacgacga ttattaccgc gtctatatcc gcacgaccga acagggacgg attgccgtta
     2341 tgcaggaaaa cgaataccgt gaagatttgg cggcggatgc ggcacggcaa agcgtgttgc
     2401 ccctgctggc ggcactgccg ctgatgatac tgctgaccgt gtggattacg cacaaagcca
     2461 tgcgccccgt ccgcaaattg tcgcaaagtc tcgaacaacg ccgaatcaat gacctgtctg
     2521 ctttgagtgt ggacaatatt cccagtgaaa tcagagggtt cgtaaccgcc atcaacctgc
     2581 ttttgaaacg tgcggatgaa gacatccgcc accgtcagcg gtttgtcgcc gacgcggcac
     2641 acgaattgcg tacgccgatg acagcccttt cccttcaggc ggaacggctc aacaatatgt
     2701 cgctcccacc cgatgcagcg cggcagtctg ccgttttgca gcagagcatc aggcgcaaca
     2761 aacacctgct cgaacagctt ttggcactgg cgcgttcgca gtcggatgaa acccctctga
     2821 cgaaaacgac attcggtctg caaagccgtt tccgccaagt gttgcaggaa ctgatgccgc
     2881 tggctttgga aaaacgtcag gacatcggcg tggcggtcgg aggcgatgtc gaagtgtctg
     2941 ccgacgaaac ggaaatctat acgctgatta aaacctttgc cgacaacgcg gtacgttaca
     3001 cgccgcccga aggcaggata gatttgggtt tcacggacga agggaaatat ctcgccgtgt
     3061 gggtggaaga taacgggaac ggcattcccg aatccgaacg cgcccgcgtc ctcgatccgt
     3121 tttaccgtat tttgggaacg gagcagcagg ggacggggct ggggctgtcg attgccgaca
     3181 cgcttgccaa aaaatacggc ggacatttgg aattgaccga cagccgacgt ttcggacacg
     3241 gtctgttgat acgggcgttg ttggacaagg aaaccctgaa atagacagat gccgtctgaa
     3301 ggcttcagac ggcatttttt acatcagcac aatatcgtat tgttcctgcg tgtaagcggt
     3361 ttcgaccgcc agagaaatcg gtttgccgat gaaatctatc agcattgcca aggattgcga
     3421 ttcttcgtcc aaaaacaaat cgatgacgtt gggggcggcg aggatgcgga aactttcggc
     3481 atcgtaacgg cgcgcttcgc ggacgatttc gcgctggatt tcgtagcaca cggtttgcgg
     3541 cgttttcaga cggcctctgc cttggcagga agggcagggt tcgcagagga cttggtttaa
     3601 gttttcgcgc gagcgtttgc gcgtcagctc gaccagcccc aggctggtaa aaccgtgcag
     3661 ggtaacgcgg gtacggtcga aggcgagggc tttggcaagc tcctgcaaca cggcttcgcg
     3721 gtggctttcc tgcgccatat cgatgaagtc gatgatgatg atgccgccga ggttgcgtag
     3781 cctcaattcg cgggcgatgg tgtggcaggc ttcgaggttg gtgcggaaga tggtttcgtc
     3841 gaagttgcgt gcgccgacga agccgccggt gttcacgtct atcgtggtca tggcttcggt
     3901 ggattcgata atcaggtagc tgccgaagtt gaggttgacg cgcggttgca gggcgcggct
     3961 gatttcctgt tcgacgttgt gggtttcaaa cagcgggcgt tcgcctttga acaattctat
     4021 cctgcccaat gcgccgtgga cgtattgttc ggcaaaacgc gtcatgcgcc cgtggtttac
     4081 ggtggaatcg acgaggattt tttgcgtgtc gcagccgacc atatcgcgca atacgcgcaa
     4141 actcaagggc aaatcctgat aaagcagggt ttccggcggc cggattttcg cctgttcttg
     4201 gatgtgttcc cacactttgg tcaggtagtc gatgtcggac tggagctgtt cgtcggtggc
     4261 gttttcggcg ttggtgcgga tgatgtagcc ccggcaggca ttttccggca ggagcttgtc
     4321 gaggcgttcg cgcaggctgc tgcgttcggc atcgtcttcg atgcgttggg acacgccgat
     4381 gtggtcttct tgcggaagat ggacgaggaa acgccccgcc agcgagattt gggtggaaag
     4441 ccgcgcgcct ttggtgttga tcgggtcttt gatgacctgc accaaaacag actgcccttc
     4501 aaacagcata tgttcgatgc gctgggtttc ttcggggttg cggcgttgtt cgaggacatc
     4561 gacgatgtgt aaaaacgccg cgcgttccaa gccgatgtcg ataaacgcgc tctgcatccc
     4621 gggcagcacg cggcgcacca cgcccagata gatattgccg accaggctgt gttcgctgtt
     4681 gcgctcgatg tgcagctcgc agatattgtt ttcctccaac accgctacgc gcgtttcctg
     4741 cggcgtgatg ttgaccaata tcgtttcggg cgggcgcgcg atgtctttgg ggatggggag
     4801 tcctgacaac atggtttttc ctgaaaatgt aataaaaata tttttgcacc cgttccgccg
     4861 cgcagcgcgg gtgcaaaaat atttttcaca atgcctatca tactttaaaa aagaaacttt
     4921 gacacattcc gcacatgccg tttgaacgtg catctgttcc gcacttgcca aacaggggaa
     4981 atgcccctat attgattatc attgcaaaac tttcggaaac ccaatatgca gaccgttacc
     5041 atgtacacag gtccgttttg tccctactgc gcgatggcga aaaggctgct gcacgcggca
     5101 ggtgtcggac atatcgacga aatccgtgtc gatgcaagcc ccgaagcctt tgccgaaatg
     5161 cagcagcttt cgggacagcg cagcgtgccg cagattttca tcggcgaaac gcacgtcggc
     5221 ggatttaccg acctctaccg cctccagcag gaaggcgggc tggacggact gctgaaccct
     5281 taacccccaa ctaggaaaac aaaatgagcg aagaactgca acccgtattc agcatcgagc
     5341 gactgtatgt caaagacttg tctttggaag tgccgcacgc gccgcaaatc tttttggaac
     5401 agggcgagcc tgaagtggat atgcgcgttt ccaccggcag ccaaaagctg gaagacggct
     5461 actacaacgt ggacgttacc gtaaccgtta ccgccaagct ggataacgag cgcacgatgt
     5521 ttttgaacga agtaacccaa agcggcatct tccgtttgga aaacatcccc gaagaagatg
     5581 tgcagctgct gctgggcgtg gcgtgtccga acatcctctt cccttacgcg cgcgaagccg
     5641 tttccggtac ggtaacacgt gccggcttcc cgcccgtcct gctcgcgccg attaattttg
     5701 aggcgattta ccaacaacag caggaagccg aagccgccgg ggcttgattc ctgcctgccg
     5761 atgccgtctg aatccgtttc agacggtatt tttgttttcg gataaaatag gtgcgttcaa
     5821 aggaatctgc ctatgatgtc gcccgaaacc cagaaacagc tcaaaatcac cgatgtttcc
     5881 gccaagaagc tcgacaaact caacctccat accgcgtggg atttggtgtt gcacctgccg
     5941 ctgcgttacg aggacgagac gcacattatg ccgattaagg acgcgccgat tggcgtgccg
     6001 tgccaggtcg agggcgaggt tatccatcag gaagtaacgt tcaaaccgcg caagcagttg
     6061 attgtccaaa tcgccgacgg ttccggcagc gtcctttttc tgcgcttcat ccacttttac
     6121 gccagccatc agaaacagac ggcggtcggc aaacgcatcc gcgccgtggg cgaaatcaaa
     6181 cacggctttt acggcgacga gatgattcat cccaaaatcc gcgatgccga gggcggcggt
     6241 ttggcggaaa gcctcacgcc ggtttacccg accgtaaacg gtttgaacca gcccactttg
     6301 cgccgtatta ttcagacggc gttggacgtt acgccgctgc acgacacgct gcccgatgcc
     6361 ctgctgtgcc gtctgaagct gccacacctc gccgaaagcc tgcgtctttt gcattcgccg
     6421 ccgccgagtt tcacgattca tcagctttca gacggcacgc tgcctgcatg gcaacggctc
     6481 aaattcgatg aacttttggc gcaacagctt tcgatgcgct tggcgcgaca gaagcgtatc
     6541 ggcggcacgg cggcggcatt gggcggcgac ggcacattga cccaagccct gcgccaagcc
     6601 ctgccgtttg ccctgaccga tgcgcaagaa aaagttgttt ccgaaatctg ccgcgatatg
     6661 gcgcaaacct acccgatgca ccgcctgctg cagggcgatg tcggcagcgg caaaaccatt
     6721 gtcgccgcct tgtccgcgct gaccgccatc gaatccggtg cgcaggtggc tgtaatggcg
     6781 cccactgaaa tccttgccga acaacatttt attaaattta aacaatggct cgaacctttg
     6841 ggcattgaag ttgtccgcct ttttggcagt ttgcgtaaaa aagccaaaga cgaagccaaa
     6901 gccaaactcg ccgacggcag cgtcaaaatc gccgtcggca cgcacgccct gttttcagac
     6961 ggcgtggcgt ttcacaattt gggcttgacc attgtggacg aacagcaccg tttcggcgtt
     7021 gcccaacgcc tcgccctcaa aaacaaaggg cgcgaagtcc atcagctgat gatgtccgcc
     7081 acgcccatcc cgcgcacgct tgcgatgagt tttttcgccg atttggacgt gtccgtcatc
     7141 gacgaattgc cgcccgggcg cacaccgatt aaaacgcgcc tcgtcaacaa cgtccgccgc
     7201 gccgaagtcg aaggcttcgt cctcggcact tgccgaaaag ggcggcaggc gtattgggtc
     7261 tgcccattga ttgaagagag cgaaaccctg caactgcaaa ctgctaccga aaccctagag
     7321 cagcttcaga cggccttacc cgaactcaac atcggcttgg tacacgggcg catgaaagcc
     7381 gccgaaaagg ccgaagtgat ggcgcggttt tcttcggggg ggctgaacgt cttggtcgcc
     7441 accaccgtca tcgaagtcgg cgtagatgtg cccaatgccg ccctgatggt catcgaacac
     7501 gccgagcgca tgggcttggc gcagctgcac caattacgcg gtcgggtagg gcgcggagcg
     7561 gcagaaagcg tgtgcgttct cctgtttgcc gagccattga gcgaactcgc caaagcgcgg
     7621 ttgaaagtca tctacgaaca caccgacggc tttgaaatcg cccgccaaga cctcaacatc
     7681 cgcggccccg gcgaatttct cggtgcgcgt caaagcggcg ttcccatgct gcgttttgcc
     7741 aatcttgaag aagacttaca ccttttggaa caagcgcgcg aaatcgcccc tttgctgatt
     7801 gaacaaaacc ctgaaatcgt cgaagcccat ttggcacgat ggctttccgg cagggaaggt
     7861 tatttgggcg tgtgagcgaa atgtcgccgg aaatattgga aaaaagtgca acggaatctg
     7921 attttgcatt atttttgaca cattttcatg attttaaatc tcgttattcc caagtgggcg
     7981 gaaatccggc aatgggaagc cgcagagact tatcgggaaa acggcaagcc ctccgccgtc
     8041 attcccgcgc aggcgggaat ctagaaatga aaaaccacgg tgttatcgga aatgattgaa
     8101 accggagaaa tgacggaatc gaagcgtgtg gaaatgtgtc ggaaattgct gaagctggat
     8161 aaactagatt cccgcttttg cgggaatgac gggattttag gtttttggtt tgttttcgcg
     8221 gcaatgatgg gattttaggt ttttggtttg ttttcacggc aatgatggga ttttaggttt
     8281 ttggtttgtt ttcacggcaa tgatgggatt ttaaattgca ggtatttacc gggaaaaacg
     8341 gcaagccctc cgccgtcatt cccgcgcagg cgggaatcta gaaatgaaaa accacggtgt
     8401 tatcggcaat gactgaaacc ggagaaatga cgaaattgaa gcgtgcggaa atgtgtcgga
     8461 aatcgctgaa gccggataaa ctagattccc gcttttgcgg gaatgacggg attttaggtt
     8521 tttgttttcg cggaataatg agccaaaagc cgtctgaacc taaaaaaccg ccgctttcag
     8581 atggcatcaa aaaccgcagg cacgatgcct gcggtttgcc gtttgcgctt caggggagca
     8641 gggggatgcc gcccaagccg tgtgtttcct tcagtccgaa catgatgttc atattttgca
     8701 ccgcctgtcc cgccgcgcct ttgacgaggt tgtcgatgac ggaaaggacg acccacacat
     8761 cggattgcgc cgcctgttgg atgctgatgc ggcagaggtt tgcgccgcgc acgctgcggg
     8821 tttcgggtgt cgaaccggcg ggcaggatgt ccacgaacag gctgtcgcgg taatagtcgc
     8881 gcaggatggt ttcggggcag atgccgtctg aaaggtggag gtaaacggtg gcgtgcatac
     8941 cgcgtatcat cggcgcgagg tgcggcgtga acacgaatcc ttcggcgatg ccgtcctgaa
     9001 gcccggcgat ggtctgcctg atttcgggca ggtggcggtg tccagctatg ccgtaggctt
     9061 tgaagttgtc gccgacttcg cacaacagcg aaccgacatt gcctttcctg cccgcgccgg
     9121 acacgccgga tttgcagtcg gcaatcagcg gcataccggg cttcagacgg cattgccgca
     9181 acagcggcac gagcggcagg gatacgcagg tcgggtagca gccggggttg gcgacgaggc
     9241 gcgcctgtgc gacggcttcg cggttgagtt cgctcaatcc gtacacggct tgggaaacga
     9301 ggccgggggc ggcgtgggtc atgccgtacc agtgttccca ggtcggaatg tcccatatgc
     9361 ggaagtcggc ggaaaggtcg atgacgcgca cgccctgttc aatcaggcgc ggcgcgtctt
     9421 tcatggcgat gccgttgggc gtggcgaaga agacgatgtc gcattgttcc aaacctgcct
     9481 cgtcgggcgt ttggaaggcg aggccgtaca cgccgcgcaa actcggaaag taatcggcaa
     9541 ctgcggttcc cgcttcgctg cggctggtta cggcggcgac ttcgacatcg ggatgggcgg
     9601 caagcaggcg cagcagttcc acgccggtgt agcccgtcgc gccgacaatg ccgactttga
     9661 tttttttact catggttttt cctttgtgtg gttggcgggt atgccgtccg aacgctgtcc
     9721 gacggggttt tgattggcgg cggaaggacg gtacacaaag cgtttgaaaa gcgtgtctaa
     9781 tttaatggca tccgcctctg tttttaaaac ctagccctgc cgtccggaat aaacataacc
     9841 gtaggcgcga aagccggcag gatatttcgg tgcctgcccc aaacccaaac cgtatgcggc
     9901 gaaagcggtg gtcgtggaga aggaaagcag gaggaggacg agggttttca taaggttcca
     9961 aaccgaacgg ggcgatgaag cccgattata gcaaaaggtc gtctgaaaac cgttttcaga
    10021 cgacctttta tttaattaca ataaaaatgt taacaacaaa aaacaaaccg cttttttccg
    10081 tttggagaca aatttttaac taaaattgtc aacaattcct tgatgcacac aaaaaaaaac
    10141 tttaatattt gtaaatatat gtaacattat aaattttaaa tattttctga gtaaggggaa
    10201 gtaatgagca tccatctcga cttcggcatt agtcctaaaa cgttccgaca gacttatctg
    10261 tatcaaaagc ccaagctctt taaaggagcg gttcggaatc tcgaatccgc atcttggaaa
    10321 gatatcaacg agatatacca acgagcagac ccaaccgcac cgctgtttca tctgcgtaaa
    10381 aaaggcgcaa tcgttcctaa agaagaatac gtcgaaagtt tcgacgattt gggcaaaact
    10441 cgctaccgtt ttattaaatc cgttatctac gaacatatga agaatggtgc gtcgttagtc
    10501 tataaccata ttaacaacga gccgttttca gaccatatcg cccgtcaagt cgcccgcttt
    10561 gccggcgcac atactattgt tagtggatat cttgcttttg gcagcgacga atcttataaa
    10621 aaccattggg acccccgcga tgtgtatgcc atccagcttt tcggcaagaa acgttggcaa
    10681 cttactgccc ctgatttccc tatgccattg tatatgcaac agactaaaga tactgatatt
    10741 tccattcctg aacatatcga tatggatatt atccttgaag caggtgatgt cctctacatc
    10801 ccacgcggtt ggtggcacag acctatcccg ctcggctgtg aaaccttcca cttcgctgtc
    10861 ggtaccttcc caccaaacgg ctataattac ctcgagtggc taatgaagaa atttcccacc
    10921 atagaaagtc tgcgccacag tttctcagac tgggagcaag ataggacgcg tatcaacgat
    10981 actgccacac aaattgctgc catgattgcc gaccccgtca attatgaagc cttcagtgaa
    11041 gactttctcg gcaaagaacg taccgatacc gcttttcatc tcgaacagtt cgcgaatccc
    11101 aacgctactc cgctttcaga cgacgtcagg ttgagattaa atgccaataa tttggatacg
    11161 ttggaaaagg gatatttgat tgggaatggg atgaagataa gcgtagatga gttggggaaa
    11221 aaagtgttag aacacatcgg taagaatgaa ccgttattgt tgaaaaatct actggttaac
    11281 ttcaatcagg gaaaatatga agaagttagg aagttgattt atcagttgat agagttagat
    11341 tttctggaaa ttttgtgagg gattctatga aaaactggaa gcaatttaca tttttcgtaa
    11401 tattagtaat agcttgttat caagtgctat attttttatc agatatgttt cttctcgact
    11461 acattaacaa atatagttgg aatttgaatt ttattcaggg tactttgaat tttttttcaa
    11521 tatatctgcc atatgttttt gttagccgaa tttttaggaa taccaaccaa gaaaaggagt
    11581 ataaaaatga ttgaacttca acttcatgaa ttgaagctgg tttcaggggg aggtcctgta
    11641 acagacaata tagctggaaa tgtagctaat gctgccacaa ccaaaggagg tcccacatgg
    11701 ggggatttgg ttgcaatacc tgctgccgca gcggcagttt attatctgcc gaaaaatgct
    11761 tacggtgctg ctggtgcaaa tggtgtatac aatgtgactc gtaattgggt aaatgatgcc
    11821 gttaatgcac ctccttataa cggaagacca atctttgaga ttgaacatgg attaactgct
    11881 cccgcaacaa aagcagataa atcaggaaac ggctacactg acggtacaga ttactgctga
    11941 tatttctcat cgtccagaca atctctaggg gtcgtctgaa actttcttaa cttcaatttt
    12001 atgaatagac ccaagcaacc cttcttccgt cccgaagtcg ccgttgcccg ccaaaccagc
    12061 ctgacgggta aagtgattct gacacgaccg ttgtcatttt ccctatggac gacatttgca
    12121 tcgatatctg cgttattgat tatcctgttt ttgatatttg gtaactatac gcgaaagaca
    12181 acagtggagg gacaaatttt acctgcatcg ggcgtaatca gggtgtatgc accggatacg
    12241 gggacaatta cagcgaaatt cgtggaagat ggagaaaagg ttaaggctgg cgacaagcta
    12301 tttgcgcttt cgacctcacg tttcggcgca ggaggtagcg tgcagcagca gttgaaaacg
    12361 gaggcagttt tgaagaaaac gttggcagaa caggaactgg gtcgtctgaa gctgatacac
    12421 gagaatgaaa cgcgcagcct taaagcaact gtcgaacgtt tggaaaacca gaaactccat
    12481 attttgcaac agatagacgg tcagaaaagg cgcattagac ttgcggaaga tcagtggcaa
    12541 aaacggcact gggcagacag gaattgtcgg gattgggcat ggtatcctcc gatttggcga
    12601 agagcaacga acctgtttat ctcgtgaaaa taaaacccga caaaccaacc atcactgcat
    12661 acggtgagga aaaaccgctg caaatcggca tgacgttgga agcagacatc ctacacgaga
    12721 aacggcgatt gtacg
//
LOCUS       DAWFBO010000043        12470 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFBO010000043 DAWFBO010000000
VERSION     DAWFBO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.043"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..206
                     /locus_tag="WGO87_000888"
     CDS             <1..206
                     /locus_tag="WGO87_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEZ0392267.1"
                     /translation="PRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEA
                     TVAYGLKHPELKDDFKELLKQFV"
     gene            289..1305
                     /gene="galE"
                     /locus_tag="WGO87_000889"
     CDS             289..1305
                     /gene="galE"
                     /locus_tag="WGO87_000889"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0392268.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     TDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVF
                     GDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAF
                     EAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNHPN
                     GYDD"
     gene            1401..2462
                     /gene="rffG"
                     /locus_tag="WGO87_000890"
     CDS             1401..2462
                     /gene="rffG"
                     /locus_tag="WGO87_000890"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0392269.1"
                     /translation="MKKILVTGGAGFIGSAVVRHIINDTQDAVVNVDKLTYAGNLESL
                     ESLANHPRYAFEQVDICDRAALDRVFAEHQPDAVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWHDMPSEKKAAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIEVVRTICALLEELVPN
                     KPQGVARYEDLITYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVEWYLNN
                     QTWWSRVLDGSYNRERLGS"
     gene            2675..3550
                     /gene="rfbA"
                     /locus_tag="WGO87_000891"
     CDS             2675..3550
                     /gene="rfbA"
                     /locus_tag="WGO87_000891"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0392270.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            3605..4144
                     /gene="rfbC"
                     /locus_tag="WGO87_000892"
     CDS             3605..4144
                     /gene="rfbC"
                     /locus_tag="WGO87_000892"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0392271.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            4306..6354
                     /locus_tag="WGO87_000893"
     CDS             4306..6354
                     /locus_tag="WGO87_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0392272.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPEINRL
                     VQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSIYFNAETPSRLEYILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYR
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQY
                     ADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK
                     "
     gene            6491..7750
                     /locus_tag="WGO87_000894"
     CDS             6491..7750
                     /locus_tag="WGO87_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ0392273.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKNTSDSSTPTT"
     gene            8119..9525
                     /locus_tag="WGO87_000895"
     CDS             8119..9525
                     /locus_tag="WGO87_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0392274.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            9743..10957
                     /gene="gltS"
                     /locus_tag="WGO87_000896"
     CDS             9743..10957
                     /gene="gltS"
                     /locus_tag="WGO87_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0392275.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            11056..12072
                     /locus_tag="WGO87_000897"
     CDS             11056..12072
                     /locus_tag="WGO87_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0392276.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            12083..12280
                     /locus_tag="WGO87_000898"
     CDS             12083..12280
                     /locus_tag="WGO87_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392277.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            12298..12447
                     /locus_tag="WGO87_000899"
     CDS             12298..12447
                     /locus_tag="WGO87_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392278.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTRRTIYKAL
                     PYVPS"
BASE COUNT         3190 a         3300 c         2982 g         2998 t
ORIGIN      
        1 tacctcgtgg tgcaggtggt gaaatccagc taactgatgg tattgcccgc ctacttgacc
       61 atgaatttgt cttggcgcat gcgtttgaag gtaagcgtta tgattgtggc agtaagttgg
      121 gctatctgga agccacggta gcttatggct tgaagcatcc cgaattaaaa gatgatttca
      181 aagaattatt gaagcaattt gtataatttt aaggccgtct gaaaaagaaa aggcatcatg
      241 ttttttacac tttcaggcgg ccttttaaac ataaagaagg aaaacgtgat gaacattctc
      301 gttaccggcg gcaccggttt tatcggctcg cacaccgttg tttctttgct gaaaagcggt
      361 catcaagtcg tgattttgga taacctatgc aattccagca tcaatatcct gccacgcttg
      421 aaaacgatta ccggccaaga gattccgttt tatcaaggcg atatccgcga tcgtgagatt
      481 ttgcgccgta tttttgcgga aaaccgcatt gattcggtga ttcattttgc cggcttgaaa
      541 gcggtgggtg aaagtgtggc cgagccgata aaatattatg ataataatgt ttccggcagc
      601 ttggtgttgg cggaagaaat ggcgcgtgcg ggcgtgttta gcattgtgtt cagttcttcg
      661 gcgacggttt atggcgatcc gggcaaagtg ccttataccg aggatatgcc accgggcgac
      721 accaccagcc cttacggcgc atcgaaatcg atggttgagc gcattctcac tgacattcaa
      781 aaagccgatc cgcgctggag catgattttg ttgcgttatt tcaatccgat tggcgcgcat
      841 gaaagcggtt tgattggcga gcagccaaac ggtatcccaa ataatttgtt gccttatatc
      901 tgccaagtgg cggcaggcaa actgccgcaa ttagcggtat tcggtgatga ctaccctacc
      961 cctgatggca cgggtatgcg cgactatatt catgtgatgg atttggcaga aggccatgtc
     1021 gcggctatgc aggcgaaaag caatgtagca ggcacgcatt tgttgaactt aggttccggc
     1081 cgcgcttctt cggtattgga aatcatccgc gcatttgaag cggcttcggg tttaaccatt
     1141 ccatttgaaa tcaaaccgcg ccgcgccggt gatttggcgt gcttctatgc cgacccttct
     1201 tatacgaagg cgcaaatcgg ctggcaaacg cagcgtgatt taacccaaat gatggaagat
     1261 tcatggcgct gggtaagtaa tcatccgaat ggttatgatg attaagtcat gaaattgaga
     1321 ggccgtctga agtttcagac ggcctttgtt tgcgatttat agtaaaattt accttatcat
     1381 tttggcgatt aatattcatc atgaaaaaaa tccttgtcac cggcggtgcc ggttttatcg
     1441 gttcggcggt tgtacgccat atcatcaacg atacgcaaga tgcggtggtg aatgtggata
     1501 agctcacgta tgcgggcaat ttggaatcgc tggaatcttt ggccaatcat ccgcgctatg
     1561 cgtttgagca agtcgatatt tgcgaccgcg cggcactgga tcgtgtgttt gccgaacacc
     1621 agccggatgc ggtgatgcac ttggcagcag aaagccatgt tgaccgcagt attgatggcc
     1681 cagcggcatt tattgaaacc aatattgttg gtacttacac gcttttggaa gcggcgcgcg
     1741 cgtattggca tgatatgccg tctgaaaaaa aagcggcttt ccgtttccat catatttcga
     1801 ctgatgaagt gtatggcgat ttgcacggca cggatgattt gtttaccgaa accacgcctt
     1861 acgccccttc cagtccttat tcggcttcca aagcctccag cgaccatttg gtgcgcgcat
     1921 ggctgcgtac ttacggcctg ccgaccattg tgaccaattg ttctaataac tacggccctt
     1981 atcattttcc tgaaaaactg attccactga tgattctgaa tgcgcttgat ggcaaaccgc
     2041 tgccggtgta tggcgacggt atgcagattc gtgattggct gtttgttgaa gatcatgcgc
     2101 gtgcgctgta tcaagtggtc acagaaggcg aaatcggcga aacttacaac attggtggcc
     2161 acaatgaaaa agccaatatt gaagtggtgc gcaccatttg cgccctgctg gaagaattgg
     2221 tgccgaacaa accgcaaggc gtggcgcgtt atgaagattt gattacctat gtcaaagacc
     2281 gccccggcca tgatgtgcgc tatgcgattg atgccgcaaa aatcggccgt gagttgggtt
     2341 ggcagccgca agaaactttt gaaagcggca tccgcaaaac agtggaatgg tatttaaaca
     2401 atcaaacttg gtggtcgcgc gtgttagacg gcagctacaa tcgtgaacgc ttaggcagtt
     2461 aaatagagac ctttgcaaac tccccatctt tggcacattt cttcgttatg cgctgctcga
     2521 aagcttgcct atctttgtga tatgtctgcg ctttctgcgc tgctataact tcgaactgta
     2581 ctcaaatctg tggctttgca aaggcctcaa ataataaaaa tacgttcaga cggcatgaag
     2641 gccgcctgaa aaactacata aggaaagtaa actgatgaaa ggcattattc tcgcaggcgg
     2701 cagcggtatc cgcctttacc cgattacccg cggcgtatca aagcaattac taccggtgta
     2761 caacaaaccg atgatttatt accccttgtc ggtgttgatg ttggccggaa tccgcgatgt
     2821 gctggtgatt accacccccg aagacaacga tgctttccgc cgcttactgg gtgatggcag
     2881 cgatttcggt attcaaattt cctacgccat ccaacccagc cccgatggtt tggcgcaagc
     2941 ctttttaatt ggtgaagaat ttatcggcga tgattcggtg tgtttggtgt tgggcgacaa
     3001 tattttctat ggccagcatt tcagccaatc cctgaaagaa gccgccgctt ccgtggaaac
     3061 caaaggtgct accgtattcg gctatcaagt gaaagatccc gagcgtttcg gtgtgattga
     3121 gtttgatgag aatttcaaag ccctgtcaat cgaagaaaaa ccgaaacaac ctaaatccga
     3181 ttgggcggta accggcctct atttctacga taatcgcgta gtcgagtttg ccaaacaagt
     3241 gaagccgtct gaacgcggcg aattggaaat taccaccttg aatcaaatgt atttggacga
     3301 tggttcgctc aatgtccaat tgctcggtcg cggtttcgca tggcttgata ccggcacaca
     3361 cgccagcctg ttggaagcag cctcgtttgt cgaaaccatt gaaaacattc aaaacctaca
     3421 agtcgcctgt ttggaagaaa tcgcatggcg taatggctgg ttgagcagcg aagaaatcag
     3481 aaacttggca aagccaatgt gtaaaaatga atatggccgc tatttattgc gcttggttgg
     3541 caaagattaa gaggttgctt ttaccagttt tcaggccgtc tgaaacgctt tttaggaaaa
     3601 catgatgaat atcatcgata cccgcattcc cgatgtcaaa ttgctcgaac ccaaagtctt
     3661 cggtgacgag cgtggttttt ttatggaaac cttccgtgat gaatggttcc gccaaaatgt
     3721 agccgagcgc actttcgtgc aggaaaacca cagcaaatcc acccaaggcg tattacgcgg
     3781 tttacattat caaaccgaaa atacacaagg caaactggtg cgcgtggtat caggcgaagt
     3841 ctttgatgtt gccgtggata tgcgtcgtga tagtcctact ttcggccact gggtcggtga
     3901 ggttttatcg gcagacaaca aacgccaatt atgggtgccg gaagggtttg cccatggatt
     3961 ttatgtgttg agcgatgaag cggaattcgt ctacaaatgc accgactatt acaacccgaa
     4021 tgcggaacat tccctaattt ggaatgatgc cacagtggga atcgactggc ctttattggg
     4081 agaaccaagc ctatcggcca aggatttggc aggcaagatg cttgctgaag cggccacatt
     4141 ttaattttca cttttaggtc atcgtgggta atttgtagca agatgaccta atttttacca
     4201 gctagcctac taactaaatt tctactatat ttaaatagtc agaaaattta cacaaaatct
     4261 gttaataaga tattatattg tcagtaatat tttttcctat ttattgtgat tcctgcctac
     4321 attcctcttc gctctttcgg tatacgtaaa attccgaata ttacgcaatt tctaccggaa
     4381 tattcactta acaaaaccgt attcaacgcc caaaccatca ttggctgggg cttacgcccg
     4441 accacccacc aagcacgtgc ttttgcacaa tcacatggct tgccgtttgt agccttggaa
     4501 gacggttttt tacgctcact gggcttaggc gtggccggtt atccgccctt cgcactggtt
     4561 tacgacgata tcggcatcta ttacgacaca acccgcccat cgcgtttgga gcagttgatt
     4621 ttagccgcaa acatgccgtc tgaaatgctt aaccaagcgc agcaggccat tgatttgatt
     4681 ttgacgcacc gtttgtctaa atataaccat gctctggatt tttcagacgg cctcgtacaa
     4741 gacagcaaaa acattgtttt ggtaattgat caaactttcg gcgatatggc gattcaatat
     4801 ggcggcgcag atgaagccac attccgccaa atgtttcaga cggccttagc cgaaaatcct
     4861 gatgcggaaa tctgggtcaa aacccatccc gatgtattga gcggcaagaa acagggttac
     4921 cttactgatt tggcacagca gccgcgtgtg cgtttattgg ctgaagacat taatccgatt
     4981 tcgctgctgc aagcggtgga caaagtgtat tgcgtgacct cgcaaatggg ttttgaagcc
     5041 ttgttgtgcg gcaagccttt ggtaacgttt ggtttgccgt ggtatgccgg ctggggtgta
     5101 agcgacgacc gccatcctga aatcaaccgc cttgttcaaa cccaacgccg cgccacccgc
     5161 aacttgctgc agctcttcgc cgcagcctat ctgcaataca gccgctacct caaccccaat
     5221 accggcgaag caggcagcct ctttgatgtc atcgactatc tggcgacggt caaacgtaaa
     5281 aacgacaaat tgcgtggcga gttatattgc gtcggtatgt ctttgtggaa acgcgcggtt
     5341 gccaaaccgt tctttaacgt accctcttgc cgtctgaaat ttatctcttc cacccaaaaa
     5401 ctggcaaggg tcaaactgtc cgacgatgca cgcatcctgg cttggggcaa cggcaaagag
     5461 gccatcgtcc gctttgccga acaacaccac atccccctgc tgcgcatgga agacggcttt
     5521 atccgctcgg tcggactcgg ctccaactta gtgccgccgc tgtcgctcgt taccgacgat
     5581 atgagcattt atttcaatgc cgaaaccccg tcccgtcttg aatacatcct acaaaaccaa
     5641 aacttcgacg atcaagactt tcagacggcc ttgaagctgc aaaaaatgct gaccgaaaac
     5701 cacatcagca aatacaacgt cggcagctca gacttcaccg ccccgtcaac cgacaaaacc
     5761 gtgatcctcg ttcccggcca ggttgaagat gatgcgtcta tccgctacgg ttcgccccaa
     5821 atctaccgca atctggattt gctccgtacc gtacgcgaac gaaaccccaa tgcctatatc
     5881 atctacaaac cgcatcccga tgtagtcagc ggtaaccgca tcggccatat ttcccctgaa
     5941 gatgctgcac gatatgccga ccaaaccgcc gaacaagccg acatcctgac ctgtctccaa
     6001 tacgcagacg aaatacatac catgacttcg ctgaccggtt ttgaagcctt gttgcgcggc
     6061 aaaaaagtca gctgctacgg cctgcctttt tacgcaggct gggggcttac ccaagatctg
     6121 ctccccatcc cgcgccgtag ccgcagactt gagctttggc agctgattgc cggcacgctc
     6181 atccactatc ccgactacat ccaccccgaa acccatcagg ccataaatgc agaaaccgca
     6241 gcccaaatcc tgatacgaca aaaaaatatg caaaaaaaca acaacggatt gcatcgcggg
     6301 tgctttgcca aaaaattagg taaaatcaaa caactatatc gatctttcaa ataaatacca
     6361 tcaaagttaa cgatgcgtca taaacttgcc tctattgcgg catcattgcc tttgcatcgt
     6421 taattctctt ggcgtatgct tgaaagttca acctaaaact attacataaa aaacaaaacc
     6481 acattgcaac atgaaacaga ccgtcctcaa aaataacctg caaaacctgc ttgaaagcgc
     6541 agaaaatatc ctgctgcttc aaggccctgt cggcgatttt tttctgcgcc ttgccgactg
     6601 gctgactgca aacggcaaaa ccgtacataa attcaacttt aatgcaggcg acgactattt
     6661 ttatccgccc actcaagcgc ataccgttgt ttttaacgac aactacgatg cctttcctga
     6721 gtttttgcaa gaatacatca ctcaacatca catccaggcc gttgtctgct ttggcgacac
     6781 acgcccttat cacgtcattg caaaacgcat tgcaaacgaa aaccaagcca gtttctgggc
     6841 gtttgaagaa ggctatttcc gcccctacta catcacctta gaaaaagacg gcgtcaacgc
     6901 attttccccg ttgccgcgcc gtgccgactt ttttcttgaa caattcccta agcttgccca
     6961 gcaagaatat aaagcgccaa cgccggtaca cggcggtttt acgcccatgg caaaaaacgc
     7021 tatccgttac tatatcgagt tgttccgcaa tccacgcaaa taccccgact acatccacca
     7081 ccgcgcaccc aatgccggcc attacctcaa accgtggtcg ctctccatcc tcaagcgttt
     7141 gaactactat attgaagaca tccaaatcgc caaacgtgtg gaagcaggca aatacggcaa
     7201 gttttttatt gttcccttac aggtattcaa cgacagccaa gtccgtatcc attgcgactt
     7261 tcccagcgtc cgcagcttcc tgctccatgt tttgagttca tttgccgagc acgcgcctgc
     7321 cgataccaac atcatcatca agcatcatcc tatggaccgc ggctttatcg actactggcg
     7381 cgacattaaa cgctttatca aagaacaccc cgaactcaaa ggccgtgtga tttatgtcca
     7441 tgatgtcccc ctgcccgttt tcctgcgcca cggtctcggc atggttacca tcaacagcac
     7501 cagcggcctg tccggactga ttcacaatat gccagttaag gttctcggcc gtgcctatta
     7561 tgatattccc ggcattactg accaaaatac cttggcagaa ttttggaatc atccgacacc
     7621 gcctgacaaa gagctgttcc atgcctaccg aatgtaccac ctcaacgtga cccaaattaa
     7681 cggcaacttc tacagtcagg tcttttttcc taacaaaaac acatccgact cttccacacc
     7741 aacaacctaa cttggcgaaa gaaaagctca ggccgtctga aaacatttca gacggcctga
     7801 aacaatcaat accttagcta ctgccatgta aataaaacac aaaaatctgc atttatcatt
     7861 aacaataaat tacaaaaaca gtataatgac cgagctgcca tgagcgcata ccgactcaac
     7921 ctgagccctt tgtaacacac aaaatatgga tatatcccta ggcaaaacaa tataacaagc
     7981 caaacatcct aaagataagc cggcaaggca atacactcta taaaactgtg ccgagcaaaa
     8041 tttttacaaa gccctcaacc ggtatcgccg cccatatgcc gcagcatccg tcttccactt
     8101 tatatccgcc cgcaaaccat gaccgccgct cctgatatcc tctaccggca agccgccgcc
     8161 cttttggaac aatccaatac cgcccaagcc ctgcccctgt tgcaacaggc ggcagagcaa
     8221 ggttatgcgg aagctgcttt cgtattgggc aaccatctgc tgcaaaacgg ccaaccggag
     8281 caggcacttt catggttgga agccgccgcg gcccaacgcc atcccaaagc actcttctcc
     8341 ctgctgcaac aacgcgaaca caacggcacc ccgaccggac agcttctcaa cgactatgcc
     8401 tggctgggtg agcaggggca ctcagaagcc caattaatcc tcatgcgtta ccacgcgcaa
     8461 cgcaacgatc cacaatcgct ctactgggcg gaacttgctg ccgcccgata tgccgcacct
     8521 gcgtattacc atctggcacg ccatcatcaa cgccaaggcg acgttgaaac agccatcgaa
     8581 caatacgaaa aagcggcagc actcggcgta actgccgcct gctggcaact tggtcaaatc
     8641 tacttctacg gtacaggtgt cagccccaac cacgcacaag ccgaacacta tctcgaacca
     8701 gccgcacaag ccggccacat cgccgcacaa acgctgctgg ctgaccttct tgccgcccaa
     8761 cgcaaacctg aagccttgga atggtatcgt cgtgccgccg ataaggaaca agcggaagca
     8821 cagtctaagc tggcccaata cgccctgacc ggcgaacttt ccgaacgcga tccgttccaa
     8881 gcggcacgat atgccaaagc cgctgccgag aaaaaccatc ctgaagccct gaaaatcatg
     8941 ggcgacctct accgctacgg tctcggtatc aaagccgaca accatatcgc gcaagattac
     9001 taccaccgtg ccgccgcgct gggttctgcc gccgcagcac aaaaactcat cagcgacgcc
     9061 gcgctgtacc atccgcaaca atacgaacaa atcaaaactg ccgccctgca acaacaacaa
     9121 accgaaacca tctaccgttt ggcggaagca caagcctgcg ccatcggccg tcccgccgac
     9181 tacaatgccg cgcgaaaaaa ttacatggaa gctgccgggt tccaccataa aaacgcagcg
     9241 gcagccttag gccgcatcta ccattacggc ctcggtacgg cgcaagatcc tcgggcggct
     9301 gcacactggt acgccattgc tgccgaacaa aaccaccctt ccgcccaata ccacctcgcc
     9361 tgtttttact atcacgggca aggtgtcggc tgtcatgttc cgaccgcctg ctactggctg
     9421 caggccgcca tcggcaacgg ccacacttcg gccgaatcat taatatccct attagaacaa
     9481 tggcgacgcg aagcacacca tgccatcgga caaaaggccg tctgaaaaga tttacactcg
     9541 cattttttga caatctttaa ctattcccct aatatttgcc agttattttt cacggacacg
     9601 ccattgtttt catttctttc tgaaaacacc ttgtccgcgc atcaatacca tgacactcgg
     9661 cggataacgc caagcgttga aacacactac atccggaaca aaaacggatg ctcggaaaaa
     9721 tatttctagg aggtgaaaca acatggaatg ggaattcaac agttattaca cactgattgc
     9781 cgccacgctc gtgttgctgg ttggtaaatt tctggttcaa aaaatcaaat tcttacgaga
     9841 cttcaatatt cccgagccgg tcgctggcgg tttggttgcc gcgattatcc tgtttgcgct
     9901 gcacgaggcg tacggcgtga gcttcaaatt tgagaaaccg ctgcaaaatg cgtttatgct
     9961 gatttttttc acgtccatcg gcttgagcgc ggatttttcc cgtttgaagg cgggcggttt
    10021 gccgctggtg gtttttaccg cgattgtggg cggatttatc ttggtgcaaa actttgtcgg
    10081 ggtcggactg gctacggctt tgggtttgga cccgctcatc ggtctgatta ccggttcggt
    10141 gtcgctgacg ggcggacacg gtacgtcagg tgcgtgggga cctaattttg aaacgcaata
    10201 cggcttggtc ggcgcaaccg gtttgggtat tgcatcggct actttcgggc tggtgttcgg
    10261 cggcctgatc ggtgggccgg ttgcgcgccg cctgatcaac aaaatgggcc gcaaaccggt
    10321 tgaaaacaaa aaacaggatc aggacgacaa cgcggacgac gtgttcgagc aggcaaaacg
    10381 cacccgcctg attacggcgg aatctgccgt tgaaacgctt gccatgtttg ccgcgtgttt
    10441 ggcgtttgcc gagattatgg acggcttcga caaagaatat ctgttcgacc tgcccaaatt
    10501 cgtgtggtgt ctgtttggcg gcgtggtcat ccgcaacatc ctcaccgccg cattcaaggt
    10561 caatatgttc gaccgcgcca tcgatgtgtt cggcaatgct tcgctttcgc ttttcttggc
    10621 aatggcgttg ctgaatttga aactgtggga gctgaccggt ttggcggggc ctgtaaccgt
    10681 gattcttgcc gtacaaaccg tggtgatggt tttgtacgcg acttttgtta cctatgtctt
    10741 tatggggcgc gactatgatg cggcagtatt ggctgccggc cattgcggtt tcggcttggg
    10801 tgcaacgccg acggcggtgg caaatatgca gtccgtcacg catactttcg gcgcgtcgca
    10861 taaggcgttt ttgattgtgc ctatggtcgg cgcgttcttc gtcgatttga ttaatgccgc
    10921 gattctcacc ggttttgtga atttctttaa aggctgattt tccgcctttc cgacaaagca
    10981 cctgcaaggt ttaccgcctg caggtgcttt tgctatgata gccgctatcg gtctgcaccg
    11041 tttggaagga acatcatgta tcggaaactc attgcgctgc cgtttgccct gctgcttgcc
    11101 gcttgcggca gggaagaacc gcccaaggca ttggaatgcg ccaaccccgc cgtgttgcaa
    11161 ggcatacgcg gcaatattca ggaaacgctc acgcaggaag cgcgttcttt cgcgcgcgaa
    11221 gacggcaggc agtttgtcga tgccgacaaa attatcgccg ccgcctacgg tttggcgttt
    11281 tctttggaac acgcttcgga aacgcaggaa ggcgggcgca cgttctgtat cgccgatttg
    11341 aacattaccg tgccgtctga aacgcttgcc gatgccaagg caaacagccc cctgctgtac
    11401 ggggaaaccg ctttgtcgga tattgtgcgg cagaagacgg gcggcaatgt cgagtttaaa
    11461 gacggcgtat tgacggcagc cgtccgcttc ctgcccgtca aagacggtca gacggcattt
    11521 gtcgacaaca cggtcggtat ggcggcgcaa acgctgtctg tcgcgttgct gccttacggc
    11581 gtgaagagca tcgtgatgat agacggcaag gcggtaaaaa aagaagacgc ggtcaggatt
    11641 ttgagcggaa aagcccgtga agaagaaccg tccaaaccca cgcccgaaga cattttggaa
    11701 cataatgccg ccggagggga tgcagacgta ccccaagccg cagaaggcgc gcccgaaccg
    11761 gaaatcctgc atcctgacga cggcgagcgt gccgataccg ttaccgtatc acggggcgaa
    11821 gtggaagagg cgcgcgtaca aaaccagcgt gcggaatccg aaattaccaa actttgggga
    11881 ggactcgata ccgacgtgca aaaagagttg gtcggcgaac aacgcaagtg ggcgcaggaa
    11941 aaaatcagca actgccgaca agccgccgcg caggcagacc ggcaggaata cgccgaatac
    12001 ctcaagctgc aatgcgacac gcggatgacg cgcgaacgga tacagtatct tcgcggctat
    12061 tccatcgatt aggggcaaac cgatgaatac cgtcccaaaa agcaggattc ccgtcaaacc
    12121 gctgcccgaa aaaaccacag acgaagccaa agtcgaaaaa tggcggcagc tcggtgcgga
    12181 acacggtttg tcgggcgaat gggcagttgc cgtcagattg ggcgaaaacg gttttaccga
    12241 agaacagatg gaaaatatcg ccaacctgtt cggcagataa agagaaaatt gacggaaatg
    12301 ccgtctgaaa ccctgttatc ggtttcagac ggcattttga ccaatacggt acgcaggcgc
    12361 aaaacagccg gcttttcctg tgttgcctat gctgatgttt caacacgcag gacgatatat
    12421 aaagcgttgc cctatgtgcc gtcctgattc ggaaagggtt acgcccctcc
//
LOCUS       DAWFBO010000044        12240 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFBO010000044 DAWFBO010000000
VERSION     DAWFBO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.044"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WGO87_000900"
     CDS             complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WGO87_000900"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0392279.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNMINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGINVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3163..3525)
                     /locus_tag="WGO87_000901"
     CDS             complement(3163..3525)
                     /locus_tag="WGO87_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392280.1"
                     /translation="MTDRPNLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILLARCPPL
                     SRLLCYPCPNHPMSKNSA"
     gene            3473..3703
                     /locus_tag="WGO87_000902"
     CDS             3473..3703
                     /locus_tag="WGO87_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0392281.1"
                     /translation="MISACPGFIHSRFGRSVMPSEPADHLFQKTPYRRNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            complement(3696..4337)
                     /locus_tag="WGO87_000903"
     CDS             complement(3696..4337)
                     /locus_tag="WGO87_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0392282.1"
                     /translation="MPSEKPMNRRIFLLGAGALLLTACGRKSARTHAKIPEGSSVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVLV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4327..4878)
                     /locus_tag="WGO87_000904"
     CDS             complement(4327..4878)
                     /locus_tag="WGO87_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0392283.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAV"
     gene            complement(4882..5400)
                     /locus_tag="WGO87_000905"
     CDS             complement(4882..5400)
                     /locus_tag="WGO87_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0392284.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5400..7061)
                     /gene="pqiB"
                     /locus_tag="WGO87_000906"
     CDS             complement(5400..7061)
                     /gene="pqiB"
                     /locus_tag="WGO87_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0392285.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7054..8514)
                     /locus_tag="WGO87_000907"
     CDS             complement(7054..8514)
                     /locus_tag="WGO87_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0392286.1"
                     /translation="MGSAVGGQPLEKIFLPDTNGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGGRADVPRLDRGEAAFCPR
                     CGHKLFRVGRHPFSGPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMVRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8864..9493
                     /locus_tag="WGO87_000908"
     CDS             8864..9493
                     /locus_tag="WGO87_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0392287.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            9668..12138
                     /locus_tag="WGO87_000909"
                     /pseudo
     CDS             9668..12138
                     /locus_tag="WGO87_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
BASE COUNT         2946 a         3006 c         3324 g         2964 t
ORIGIN      
        1 gaggcaacgc tgtactggtt tttgttaatc cactatattt gtttgtccgt gtggaaatga
       61 catcaaaggg tgtgaatagg cttttcatct ttgaaatctg tttgcacgat cggatgaata
      121 aaaagtagga tgataaaaaa gctgatgtat ggatactggt ttaactcatt tgttttaaag
      181 ttatagtcga gatacgtgta ttggtgcaac cagtctgccg ttgacgaaaa tgccgtctga
      241 aaatgtttca gacggcattt gtcttcgggg tttcaacggt cagtcttcat aattttccat
      301 cggtgggcag ctgcacacga gattgcggtc gccgtacacg tcgtccacac ggttgacgct
      361 cggccagaat ttgtgttcgc ggacgaatgg caacggaaag acggcttctt cacgggagta
      421 cggatgcgcc cattcgccgg ttacatcgga tgcggtatgc ggagcgttga ccagcggatt
      481 gtcctctttc ggccattcgc cgcgcccgac tttcagcact tcctgtttga tttgtttcag
      541 ggcggcgatg aagcggtcga gttcggcttt gctttcgctc tcggtcggct cgatcatcag
      601 cgtaccggca acggggaagg aaaccgtcgg ggcgtggaag ccgtagtcca tcaggcgttt
      661 ggcgatgtcg gtttcagtga tgccgctttc ggctttgagc ggacgcaaat cgacgataca
      721 ttcatgcgcg acgcggccgt ttttgcctgt atacagaatc ggatagtctt cgctcaggcg
      781 tttggcaacg tagttggcgt tgagcaatgc ccagcgtgtc gcctgttcca tgccttgttt
      841 gcccatcatg gtcaggtaca tccaagtaat cggcagtatg gacgcagaac cgaaggccgc
      901 agcagcaacg gcggtttggt cggcactggc gctgtgggtg tcggtcaaag cgtgtcccgg
      961 tgcgaacgga gccagatggg ctttcaagcc aatcggaccc atgcccggac cgccgccgcc
     1021 gtgagggata cagaaggttt tgtgcaggtt catgtgcaac acgtccgcgc cgacttcggc
     1081 aggctgcatg atgccgattt gggcgttgag gttggcgccg tccatgtaaa cctgtccgcc
     1141 gttttcatgg acgatgcggc agatgtcgcg gatgccttct tcgtacacgc cgtgggtgga
     1201 cggataggta atcatgatgg cggacaaagc gtcgcggtgt tgctcggctt tggctttcaa
     1261 atcgtcgatg ttgacgttgc cgtgttcgtc ggtatcgacg acgacgactt tcaaaccgag
     1321 catggcggcg gtggcggggt tggtgccgtg ggcggatttg ggaatcagac agatgttgcg
     1381 ctgcgcttcg ccttgcgctt cctgatagcg gcggatggca agcataccgc tgtattcgcc
     1441 ctgcgcgccg gagttgggct ggaaggaaat tgcatcaaaa ccggtgatgg atttcaggct
     1501 gttttccata tcggcgagca attcgcggta gccggcggtt tgcgcttcag gagcgtaagg
     1561 atggatgtcg gagaactccg cccaagtaat cggcaacatt tccgcagtcg cgttgagttt
     1621 catggtgcag ctgcccaatg aaatcatgct gcggttcatc gccaagtcgc ggtcttcgag
     1681 ttttttcagg tagcgcagca tttcgtgttc ggtgtggtaa cggttgaaca cgggatgttg
     1741 cagaatgtcg tcttgacgca gcagttcggc gttcagacgg cctttgacat catcggcaaa
     1801 tgcgaacgta tccttgccgg taaacgcgcg gtacaaatca accaaatctt cgcatgccga
     1861 tgtttcgtgg aatgcagccg caacttgagt atcgttaacg cggcgcaggt tatagcccga
     1921 ttccaaagca gcggcaaaca cttggtctgc tttctctttg ttgccgaaat cgacggtaac
     1981 ggtatcgaag aagactttgt gaaccacatt aatgccgtct gaaaccagcg catcggcaaa
     2041 agcagaagcc agcgcgtgaa tgcggttggc gatgcgtttc acgccttcag ggccgtggta
     2101 aacggcgtac atacccgcca agttcgccag caatgcctgc gcggtacaaa tattggatgt
     2161 cgctttttcg cggcggatgt gttgctcgcg ggtggacaac gccatgcgca aggcaggttt
     2221 gcccgatgcg tctttggata cgccgatgat gcggcccggg gcggaacgtt tgaactcgtc
     2281 tttaaacgcg aaataagcag catgcggacc gccgaagccc atcggtacgc cgaagcgttg
     2341 ggtgttgccc aaagcaatat ccgcgcccaa ttcggcaggc gatttcagca aaaccaagct
     2401 cataatgtcg gcggaaacgg ccacgattgt gcctttggct ttcagacggc cgataacgtc
     2461 ctgcaagtct tgcacatcgc cgtctttgcc gacgtattgg aacagcgcgc cgaagtattc
     2521 gccctcgtcg gcttgagcaa aatcgccgac caccaattca aagccgaaat atttggcgcg
     2581 ggttttcatc acgtccaaag tttgcggata cacgcgcgca tccacgaaga aacgctcgga
     2641 tttcactttg cccacgcggt gcgccatcgc catcgcttcg gcggcggcag tcgcttcgtc
     2701 cagcaaagac gcgcctgcca cggggaagcc ggtcaaatcg atacacactt gttggaagtt
     2761 caacaacgct tccaaacgcc cctgggcgat ttccgcctga tacggcgtgt aggcggtgta
     2821 ccaacccgga ttttccaata cgttacgcaa aatcacgttc ggcacgcggg tcgggtaata
     2881 gcctaaaccg atataggatt tgttgatcat gtttttcgac gcaatgcctt tcaatttcgc
     2941 caatgcgtcc gcttcggtca gggcttcggg caaatcgagt tcggacggca tacggatgct
     3001 ttgcggcacg gtattgccga caaaatcgtc catgcttttc tcgccgacag cggcaagcaa
     3061 cgccgcttcg tcgccgaaac tcaaatgccg cgcggcaaat tcgtcggggt tgaacagttc
     3121 ggacagtttc atttctactc cttcgggttt gtagggctgc catcaggcag aatttttaga
     3181 catcggatga ttcgggcaag ggtagcacaa aagacgggat aatggcggac aacgcgccag
     3241 cagaatgcag caggtcagga caagcagcgc cacaagcggc agcgataagg gaaagccgcc
     3301 gcccaaaacc atgaaacctg caggcaccag ccaagccaat ggatacgccg gaagcggaaa
     3361 aacaatattg agcagaacca aaatcacgac aaacagcggc agacccgcca tcaggttggc
     3421 acgcctgtat gttgcgtacg ggtcggatgc ttcttcaggt tcgcaatatt ccatgatttc
     3481 agcctgtccg ggctttatcc acagcaggtt cggacggtcg gtcatgccgt ctgaaccggc
     3541 agaccatctt tttcaaaaaa cgccataccg ccgcaacttc atgcggctgt atggcgtagg
     3601 ttttgcactt cagacggcat atgcgtcccg aagcagtttg gtgttccagt cggttcatga
     3661 ttatataaac aacttgggtg cggataaacc tttgtttatc taaacccctg ttttctcaaa
     3721 aattgattca aatcttcggc aaatttccga tagcctttgc cgttggcgtg gatttggtcg
     3781 gatttcagat tattatcgcc caaaatttcc gcccacgcgc cgccgaacag cggaatgccg
     3841 tattcctcgg acaaatcctc atacagcgga tgatcgctca aatgtccgaa caacgcgccc
     3901 agtgtgatgt gcggcacgcc gacgaggacg gcggggatgt tttccttctg cacggtttcg
     3961 atgattttcg cgatattggc gcgggtctgc tccttgggaa ctttgcgcag aaagtcgttg
     4021 ccgcctatgc cgacaagcac aagcttgggt ttgcgtgcca acagcgcggg caggcgcgac
     4081 agggcttggg cggatgtatc gcccgatacg ccgccgttga caatattcca acccgtcagt
     4141 ttttgcagtt gcgcggggta ggattcgcca gggtttgcgc cgtagccgaa ggtaagcgaa
     4201 tcgcccaagg caagtacgga gcttccttcg ggaattttgg cgtgggttcg ggcggatttt
     4261 ctgccgcagg cggtaagcag caacgcgcct gcgccgagga ggaagattct tctgttcatt
     4321 ggtttttcag acggcatcga gccatttcgg gtggtatttc agggtttcgc ggtaaaccgg
     4381 acagctttcg gcgtgcgcgc ctttgaggta gccggtactc atcagaaatt cgccgacgat
     4441 ttcgccgccg acgaatttga aatgtttttt aaagagtttg acccattcgt ctttgcttcg
     4501 cggatggtgc gcgtcaagcc agttcttgaa cgagccgtat tcttgttgca acgcttggat
     4561 ttgccgtgca ttgaaaatgg cggcatcgat tttcaggcgg ttgcggacga tgcccgcatc
     4621 ggcaagcagc cgctcaatat cggcttcgtc aaatgcggca accgtatcga tgtcgaaccc
     4681 ttcaaatgct gtctgaaacg cctgccgctt cttcagcatc agcgtccagc tcaatcctgc
     4741 ctgattgatt tccaacacca gccgttcaaa caattcattg tcgtcctcaa tcggaaaacc
     4801 gtattgcgtg tcgtggtaat gtttgttcgg gttgtcggtg ttttcgggaa gtgaggcggc
     4861 aaattcgcaa tagttcacgg tttactcgac catctgttgc gccgcctgtt tcagtccctg
     4921 ttcgagtgcg gcggtcatcg cggcgtagcc gtcaccctgc tgttcggttt cgatatggaa
     4981 gggtctgttc gtaccgtcgg gtaggacggc gtagccgctg atgagggttt tgcccgtgta
     5041 gctgccttgg aatgcgtcaa tatagaccgt ccatttttcg gtactgccgc tgcgtgaggc
     5101 aggaacaaag atgcgtgtgc tgtccaaacg gttgaatgca ttgctcaacg ccgcttcgag
     5161 catatcgtcc aaggtgtccg cccaaacgtg gttttgtgcg gtgttgaggc ggtaggggtc
     5221 ggtttgatag accagtccgc cgcgtttgag cggttcggca aggcggactt cgacggcggt
     5281 ttcgccgcct tgcgttgcag ggcggatgta gcggctgtcg ggcaacacga aatattgtgt
     5341 gctttgcaca gtaccgcagg cggcaagcgt cagggcggcg gcgatgggga agaggcgcat
     5401 tatcggcttc ctttcgggat agggtctttg ctgctgctgt tgaaaatcag cgcgttgggt
     5461 ttttctttca aagtgttaat gacgggttga acgtctttta aggttttgtc caaactttgc
     5521 agcgtatttt gtacgtcgcc gtagataggc gattgaggcg atacgccttg cagggttgtg
     5581 cgcaactctt tcagggtttg gttcagttcg ttcggaatgt tttgtgtctg cggtttgccg
     5641 accagtttgt cgatggagct tagggcggca ttggcagatt tgagtgtgga tttgagctcg
     5701 gcaagcgaac cgttcaattc ggcaaccgtc ttatctaaag gcagtttgtc gaacttgtcc
     5761 agcaaatccg ccaatttgac ctgcaaatcg tccaaaccgc cgccctgggt cgcgataacg
     5821 gtatcgcctg cataaacggt atgcggtcgc agcttgggcg aagacgaagg ctgatcgttc
     5881 aactcaatca ttttgctgcc ggtcagcagg ttgttgctgg agatggtggc ggtcaggcct
     5941 ttgtttaagg ccgtctgaaa ttgttgtttc caatgttctt tgctttgctc gtcggcattg
     6001 atttccaaac gggaaggctc gatgcggatg cgtacgggaa tccaaccgtt ttcaaacaga
     6061 tgcaggctgt cgttgcggtc gaaataaggg acatcggaaa ccacgccgac attcagccct
     6121 ttgtactcga cgggcgaacc gacggtcagg ccgcgcacgg attgtttgaa aaacgcggtg
     6181 tagtacagcg agcggtcgtc aggcaggttg gcgacttcgc tgcggctgtc gtaaagcgtg
     6241 aagctgtctt cgcttttgac gtttttactg tttttggttt tcggcgaatc aaatgaaatc
     6301 gcgcccgaca gcagggcagg cagaggggcg gaattgagtt tgatgccgct gcctgtggtt
     6361 tcgatattga tgccgctttc cagccagaaa cggctggcgg aatgaatcag tttgtcgttg
     6421 gggctttgga tgaagatggt gtaatgcacg ctttggtcgg acgggtcgaa atgcgcgctt
     6481 tcgacttgcc cgaccataaa gttttcatac aaaacagggc tgttgacgtt gaggatgcgg
     6541 tcgtttttac caatcaaatt caagcgcagc ccgctttgcc cgatggcggt aacgggcgga
     6601 atgtcctgca cttggaacac gtcttttgcc tcgtcgcttt tgccgggtgt aaaagcgatg
     6661 tacgaacccg aaagcagcgt acccaaaccg gttacgccgc tttggtcgat acgcggcttg
     6721 accacccaaa actgggtatc gctgcggatg aggccggata cgtccgcatt gagttgggcg
     6781 gttacttcca cgcctttttg gtcgtcgcgc agtttgattc gggtaacgcg tccgacatcg
     6841 atgctcaata ccttaatgac cgtattgttg acctcaatgc cttccgcgct gtccatcaag
     6901 agcgtaacca caggccccct gttgcggatt tccttaaccc aaagccagcc gccggcaatc
     6961 agcgcaatca gcgggaccag ccagacggcg gagagcaagg tgttgttttt gcggacgcgt
     7021 gcttgggcgt gtccgtttgg aggagggctg ttgtcagtca tatttttccg tttcattgaa
     7081 agcaatgccg tctgaagcgc gtttgtccca aagcaggcgc gggtcgaaat aataggcgga
     7141 cagcatcgtc agaatcacga ccaggcagaa atagactgcc gcactgcccg gaatgacgcg
     7201 cgcggcataa gtgtggaacg aacacatcaa aataataatc acaaaaatat caatcatcga
     7261 ccagcggccg actgcttcgg tgatgcggta gaggtgcgac aatttctttg cgcccgttgg
     7321 caaagcgaag cgggcggacg caatcaaaac cgacattgcc gcaatcttca gtaccggcac
     7381 caaaatactc gcgctgaaaa taaccgccgc aatcagcctg tcgccctcgt cccacatata
     7441 agcgatgccg ttaaggatgg tattgacctc tgtggcggca ggattggacg aaatcataat
     7501 cggcaggata ttggcaggga aatacaaaat aaccgctgcc gtcagaaacg ccgacgaaat
     7561 actcagactt ttcggccgtc ggcggtacag ttccgcgccg cacaccccgc agggggattc
     7621 ggcactgtcg cggaaataca ggcagcggct gcaacaggtt ttgccttccg atgccgtctg
     7681 aaccgcatta tcccccgtca gccgcccgat ttgaaaatac acccaatgct ggggaaccga
     7741 taccgaagtc cgaatcagca taaccgacag cgcgaacatc agataaaacg ccgacccgaa
     7801 gcgaacctct gccacagacg agagcttgat atacgccacc aaagtggaaa caaaaaacac
     7861 atccaccatc atcgcctgcc tcaagcgcac catcacacgc gttgccaaac gcagcgcagg
     7921 atacgcctgt ttccgtatca gcgcggcata gacatacagg cacagcagca gaaacagaac
     7981 cggcgcgccg aaagtcagca caaacatcac ttcggccaaa aaaccataat cctgaaacac
     8041 catcaggcgt accatttcgg gtagcgaaag gacggatgcc gcacccggta tcccgacctc
     8101 gatatacgtc ataccgtaag caaacgccat caaaatcagc gaagccgccg cataggcggg
     8161 cgggccggaa aaaggatgcc tgcccaccct gaagagtttg tgtccgcaac ggggacagaa
     8221 cgccgcctct cccctgtcca accggggcac atccgcgcgg ccgccgcatt ccggacaatc
     8281 taccgtatgt ggcggcaggg cttcgtcgcg gcagttgccg cgaaggctgt gggggatatt
     8341 ttcggcaaac ggcttcatcg ggaaagtggc aaatataagt ggcggcatta taaaggaaaa
     8401 aaggcgatac acccaatcag gatgcaaaac cggcaggttc ggcagtatga acaaaccatt
     8461 ggtgtccggc aaaaatattt tttcgagcgg ttgtccgccg actgccgaac ccatttgata
     8521 aaatcccgat tctgtaaaga atagtaaaat acctgcttaa aaacatcacg caaatgatat
     8581 tttttccaaa aatatgtgat aataaatttc gtttatgatt ctttattatt tttatagtgg
     8641 attaacaaaa atcaggacaa ggcggcgaag ccgcagacag tacaaatagt acggaaccga
     8701 ctcacttggt gcttcagcac cttagagaat cggtctcttt gagctaaggc gaggcaacgc
     8761 cgtactggtt tttgttaatc cactatataa tccactatat atgcgggcaa gtgcggattt
     8821 tgcccgcccc gcatccttat cccttaccga caaaggaaaa cttatgagtg aaaccgaaaa
     8881 tcaagccctg acgtttgcca aacgtctgaa ggcggatacc acggcggttc acgacagcgt
     8941 ggataatctc gttatgtctg tccaaccgtt tgtcagcaaa gaaaactaca tcaaattttt
     9001 gaaactccaa tccgttttcc acaaggctgt cgaccacatc tataaagatg ccgaattaaa
     9061 caaagccatt cccgagctgg aatacatggc gcgatacgat gccgtaacgc aagaccttgc
     9121 agatttgggt gacaaacctt acgaatacgg caaaccgctg ccgcatgaaa ccggcaacaa
     9181 agcaatcggc tggctttatt gcgccgaagg atccaatttg ggcgcggcat ttttgttcaa
     9241 acacgcccaa aaactcgatt acaacggcga acacggcgcg cgccacctcg caccccatcc
     9301 cgacgggcgc ggcaaacact ggcgcgcctt cgtcgagcat ctgaacgctt tgaacctgac
     9361 tcccgaagcc gaagcggaag ccatccaagg cgcgcgcgaa gcctttgcat tctacaaagt
     9421 cgtgttgcgc gaaaccttcg gcttggcagc cgatgccgaa gcaccggaag gaatgatgcc
     9481 gcacaggcac taaaaaataa tcgaaccaaa taaacaaggt ctcggcatag ctgtttgcag
     9541 ggacctttaa ttacacggcg cggctttgtt tacatggatt actgtcttat taaatattaa
     9601 tgattatcat aaaatctatt attcgctaac cgatggatga acaatccata catcttgagt
     9661 tgataatatg aaaccattac aaatgctccc tatcgccgcg ctggtcggca gtattttcgg
     9721 caatccggtc ttggcagcgg atgaagctgc aattgaaacc acacccgtta aggcagagat
     9781 aaaagcagtg cgcgttaaag gtaagcacaa tgcatctacc actgtggaac gagtaaaggc
     9841 taaaaccatt caagagcaaa tgattcggga tacacgcgat ttggtgcgtt attcatcaga
     9901 tgtcggtgta tccgatgatg gtcgccgtat gaaaggcttt gccatgcgcg gggtggaaga
     9961 caaccgtgtc ggtatcagta tcgacggcgt ggcattgccc agttcggaag aaaattcact
    10021 ttatgcacgt tacggcaact tcaataattc ccgtttaagc atcgataccg aattggttaa
    10081 gggtgtggat attacgaaag gtgcggattc atttgaaagc ggcagcggct ctttgggtgg
    10141 taatgtgaac taccgcacgc ttgatgcacg ggatattgtt ttgccggaac aaaatttcgg
    10201 tgcacttctt cgcagtggtt atgcaagtaa aaacagagag tgggcgaata ccgcaggttt
    10261 cggctatgcg ggcaatgttt ttgatgccgt ggttttgtat tcccgccgac acgggcatga
    10321 aatgaaaagc gggggggatg tcgttgattt tgtagggttt ggtgaattgg ataagcagga
    10381 tcaggcagag cgcggatcgg cacgcattca tccagatccg tccaaacaca ataaccacag
    10441 ttatttggtg aaattaggct ggcaaccggc cgactctcac cgcttcggtg tttctgttaa
    10501 tggacaaaat aacagcaatt acacttatga aaaaacttac tccctgacaa gttattggcg
    10561 ggaggcagac gatgtgcaga aacgcaccaa tattaacctg aaccacgagt ggacacctgc
    10621 atcttccgtc ctttctttgt tacgtacgga tttggactat cagaaaaccc gcaatggggc
    10681 gataaactat aaaggtgata tggccgtacc ggagactggc gcacaggtta tcagtatcac
    10741 aacggcttta tggctgatag agacgagcgg aatatggata ccaaatacaa gcgcattacc
    10801 ttccgtttgg atagccggcc tttctcactg tgggcaggtg aacacagatt atcttttaag
    10861 acttttgcca gtcgtcgcga ctttgaaaat gtaaatgatg atttagattt taattcaaca
    10921 ggcaatataa ctgaccgtaa acattacacc attatacgtc cgatgaagac ggatcaaatc
    10981 ggtttttcat tgcaggataa aatcgctttt gacgacaagt tatctgtaaa tgcaggcgtg
    11041 cgctacgact atgagaaggt aaaaccgcag gctttcaagc cagatacgcc ttgtctgagt
    11101 acctgtgtta atgacggtaa tccgtccgca agaagtttta atacatggaa cggcgtttta
    11161 ggtttggact acctctttaa cgatacttgg cgtgcagctt accaaatcaa tacggggcat
    11221 cgcgtgccga cagcatcgga aatgtatttt acttatacca gcccttacgg taattggatt
    11281 gctaacccat cgttgaaagc agagcgcagt ctaaaccaaa ctctgtcact gcgtggaaag
    11341 aatgacaaag gattgctgga ttttagcctg tatcagaccc gttaccgtaa tttcttattt
    11401 gaggaagaaa aaacgtatca ggtgccgaat gagtattata atgaacatag ttgctattat
    11461 aatcctaaat attgtaaccc tacgcttacc gaaatcggcc aacaaatggt caatattgat
    11521 aaagccaggg tgaggggagt tgaatttaaa ggtgagttga acctacatca agtgatgtct
    11581 gtgccggaag gttttaaagt atccggcgca ttgggttata gtaaaggcaa attgtccggt
    11641 gaacgcggca gtttgttgtc catccagccg ctgaagttgg tgcttggatt cgattatgag
    11701 tctcctgatg agcgttgggg tattttctcc cgtgtcagct atatgggcag gaaaaaagcc
    11761 aaagatgccc aagtaattga acaaaagagc tattgcacaa gctataaact cgatccttgg
    11821 acaggtaagg aaacagatga ttgtgctgtt gccttgcagt atcgtgacga taaaatcgac
    11881 ttccgttggt tgaataaagc ggcaactgtt gtggatgtat tcggctacta caaaccggta
    11941 aaacgtatga ctttacgggc gggcgtttat aatctgttta accggaaata ccatacttgg
    12001 gacagcttgc gcggcatcaa tccgagatcg actattaact ctttaagcgt atcgaatact
    12061 aaatcagcaa atcagggtct ggaacgctac tacgcccccg gccgcaacta tgccgtatcg
    12121 ctggaatgga agttttaatc tggtattatt gaattaatcg ccttgttgaa aattaaagcc
    12181 gtccgaattg tgttcaagaa ttcattcgga cggcttttta ccgtaaatct gtgtgatggg
//
LOCUS       DAWFBO010000045        12195 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFBO010000045 DAWFBO010000000
VERSION     DAWFBO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.045"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            207..944
                     /locus_tag="WGO87_000910"
     CDS             207..944
                     /locus_tag="WGO87_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0392288.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            947..1633
                     /locus_tag="WGO87_000911"
     CDS             947..1633
                     /locus_tag="WGO87_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0392289.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1792..2655
                     /locus_tag="WGO87_000912"
     CDS             1792..2655
                     /locus_tag="WGO87_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392290.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2717..3286)
                     /locus_tag="WGO87_000913"
     CDS             complement(2717..3286)
                     /locus_tag="WGO87_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0392291.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3444..4304
                     /locus_tag="WGO87_000914"
     CDS             3444..4304
                     /locus_tag="WGO87_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0392292.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTLETFDHI
                     LKQLGIDYRP"
     gene            4439..4777
                     /locus_tag="WGO87_000915"
     CDS             4439..4777
                     /locus_tag="WGO87_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392293.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5145..5498
                     /locus_tag="WGO87_000916"
     CDS             5145..5498
                     /locus_tag="WGO87_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0392294.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5488..6354
                     /gene="atpB"
                     /locus_tag="WGO87_000917"
     CDS             5488..6354
                     /gene="atpB"
                     /locus_tag="WGO87_000917"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0392295.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6411..6647
                     /gene="atpE"
                     /locus_tag="WGO87_000918"
     CDS             6411..6647
                     /gene="atpE"
                     /locus_tag="WGO87_000918"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0392296.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6718..7188
                     /locus_tag="WGO87_000919"
     CDS             6718..7188
                     /locus_tag="WGO87_000919"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0392297.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7193..7726
                     /locus_tag="WGO87_000920"
     CDS             7193..7726
                     /locus_tag="WGO87_000920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0392298.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKM
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7737..9284
                     /locus_tag="WGO87_000921"
     CDS             7737..9284
                     /locus_tag="WGO87_000921"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0392299.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9309..10184
                     /gene="atpG"
                     /locus_tag="WGO87_000922"
     CDS             9309..10184
                     /gene="atpG"
                     /locus_tag="WGO87_000922"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0392300.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10222..11619
                     /gene="atpD"
                     /locus_tag="WGO87_000923"
     CDS             10222..11619
                     /gene="atpD"
                     /locus_tag="WGO87_000923"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0392301.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVNNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11630..12052
                     /locus_tag="WGO87_000924"
     CDS             11630..12052
                     /locus_tag="WGO87_000924"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0392302.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         3001 a         3115 c         3146 g         2933 t
ORIGIN      
        1 ttttgttaat ccactatatt gtccgaaatg gttgcccgct tcctgcctta ttgacaaaaa
       61 aacgctttcc cgataatatc ctacgaaaat taacctgccg atttgacaca gcttgcgggc
      121 ataacagcta aagcgttccg acaatttcag ctttatcttc cgcgcccgtt gtgtccgaca
      181 tcgggctttg ttgtatggga aagacaatga ttattttgga caaggtttcc aagcattacc
      241 aaacgcgcga caagacccgt tttgccgctg tcgagccgac cagcctcgaa atccgcgacg
      301 gtgaaatctt cgggctgatg ggttattcgg gtgcaggcaa atccaccctg ttgcgcctga
      361 ttaacctgtt ggaacgtccc gacagcggca aggtcaacgt ctgcggacaa gagctgaccg
      421 cgctcgatgc cgccgcattg cgtcaggctc ggcagaatat cggcatggtg tttcagcagt
      481 tcaacctgtt gaacaaccgc accgtcgccg gcaacgtcgc cttcccattg gaaatcgccg
      541 gatggccgtc tgaaaaaatc aaagcgcgcg ttgccgaatg tctcgaaatc gtcggactga
      601 ccgaacgcgc cggccactat cccgcccagc tttcaggcgg gcagaaacag cgtgtcggca
      661 tcgcccgcgc actcgcgccc aaaccccaag tcatcctcgc cgacgaaccc acttccgccc
      721 tcgaccccgc caccacgcgc agcgtcttgg aatgtttgga agacatcaac aaacgattca
      781 acgtaaccat cgtcatcgta acccacgaaa tgagcgttat cagacgactg tgcgaccgcg
      841 ccgccctgtt ggataaaggc aaagtcgtcg aaatcgtcga agtacgcggc aaccaaatcc
      901 acgcccgatc cgacatcggg cgcgaactga ttcgggagga ctgaatatgg caggcttaac
      961 atttgaacaa accgtttcca acatcatcgg tatgaaaggc gaaatcgtcc gcgcattggg
     1021 cgaaaccttc gtaatggtcg gtttgtccac cacattcgcc gtcgttttcg gcacgttgct
     1081 gggcgtgttg ctcttcgtaa cttccagccg ccaactgcat tacaacaagc cggtgaactt
     1141 cctgctcgac aacctcgtca acctcatgcg cgccttcccc ttcgtcattt tgatgattgc
     1201 gatgataccc gccacacgcg ccatcgtcgg cagcaccatc ggcccggttg ccgcctcgct
     1261 ggtgttgagc gtgtcgggat tgttttattt tgcccgactg gtggaacaaa acctgcgcga
     1321 agtccccaaa ggcgtaattg aagccgccgc cgcgatgggt gcgccgccgg ttgccatcgt
     1381 ccgcaaagtc ctcttaaacg aagcgcgcgc gggcatggtt tccagcatta ccgtgcttgc
     1441 catcgggctt ttgtcataca gcgcggcggc agggatgata ggcggcggcg gcttgggcga
     1501 cctcgccatc cgctacggct actaccgcta ccaaaccgaa gtcatcgtct tcatcgtcgc
     1561 cctcctcgtg ctgctggtca tcctgattca aagcaccggc aacgcattgg cgcggaaatt
     1621 ggataaacgt taaacccgaa tgccgtctga acgccaaaac ccccaccgct atccgaaaaa
     1681 tgctataaaa tccccctgtt cgcggcaaat gccgtctgaa cgccgaatct ggacggcagg
     1741 actccctgcc cgtcattttt gtttgaaact gccacaacat caggagaaaa tatgaaaacc
     1801 ttcttcaaaa ccctttccgc cgccgcactc gcgctcatcc tcgccgcctg cggcggtcaa
     1861 aaagacagcg cgcccgccgc atccgcttct gccgccgccg acaacggcgc ggagaaaaaa
     1921 gaaatcgtct tcggcacgac cgtcggcgac ttcggcgata tggtcaaaga acacatccaa
     1981 cccgagctgg agaaaaaagg ctacaccgtc aaactggtcg agtttaccga ctatgtgcgc
     2041 ccgaatctgg cattggctga gggcgagttg gacatcaacg tcttccaaca caaaccctat
     2101 cttgacgact tcaaaaaaga acacaatctg gacatcaccg aagtcttcca agtgccgacc
     2161 gcgcctttgg gactgtaccc gggcaagctg aaatcgctgg aagaagtcaa agacggcagc
     2221 accgtatccg cgcccaacga cccgtccaac ttcgcccgcg tcttggtgat gctcgacgaa
     2281 ctgggttgga tcaaactcaa agacggcatc aatccgctga ccgcatccaa agcggacatt
     2341 gccgaaaacc tgaaaaacat caaaatcgtc gagcttgaag ccgcgcaact gccgcgtagc
     2401 cgcgccgacg tggattttgc cgtcgtcaac ggcaactacg ccataagcag cggcatgaag
     2461 ctgaccgaag ccctgttcca agaaccgagc tttgcctatg tcaactggtc tgccgtcaaa
     2521 accgccgaca aagacagcca atggcttaaa gacgtaaccg aggcctataa ctccgacgcg
     2581 ttcaaagcct acgcgcacaa acgcttcgag ggctacaaat cccctgccgc atggaatgaa
     2641 ggcgcagcca aataaggcag tcgtataaaa tgatgccgtc tgaaccgtat ccgtgttcag
     2701 acggcatttt tgtcctttaa tccgccattc cctgccattc cgccgaatcc ggcgtatcga
     2761 ttccgaacag cgacaaagcg tgtgcaacac tgtgcgccac tatgtcgtcc gccgtctgcg
     2821 gtttgcggta catcgcagga acagggggaa acaccacgcc gcccatttcc gttacccgct
     2881 tcatattgtc caaatgggca aggttcagcg gcgtttcgcg caccatcagc accagccgcc
     2941 gcctttcctt caaaaccaca tccgccgcac gcgtcaagag gttgtcgccg aagccgtgcg
     3001 cgaccgaggc aagcgtccgc atcgaacagg gggcgaccag catcccgtcc gttttaaacg
     3061 taccgctggc aatgcacgcc ccgatattgc cgatcggatg cacgaagtcc gccaaggcat
     3121 atacctcatc tctcgcataa gccgtttccg aagcgcgcgc catctccgca cctttcgata
     3181 ccacaaggtg cgtttcgata tcttgcgcgc gtaaaagttc caaagccttc acgccgtatt
     3241 ggaaaccgct cgccccactg atgccgatta tcaaacgccg taccatcatc cgcctttccc
     3301 ataaaaccgc ctgcaacggc aaaccggcta ttatagtgaa aaaacagaaa tccgataaat
     3361 acggatacaa attgtcggca acacccaata tccgataaaa tatccgattt aacatcctat
     3421 ctgaataggc acgggagggc ggtatggcaa aagtaaaagg cggattgggg cgcggtttgg
     3481 attcgctgct cgccaacggc gcagacaaca gcagcggcga ccgattgacc acggttgcgg
     3541 ttaaagatat ccggcccggc cgctatcagg cgcgtgttca aatcgatgac gaagccttgc
     3601 aggagctggc ggattcgatt aaagcacagg gagtgataca gcccgtcatc gtgcgcgaac
     3661 acggactgtc ccgatacgaa ctgattgcgg gggagcggcg ttggcgtgcc gcacagattg
     3721 ccggcctgac cgaaatcccc gccgttatca aaaccatcag cgacgaaacc gcgttggcaa
     3781 tgggtttgat cgaaaacctc cagcgcgaaa acctcaaccc catcgaagaa gcacaaggct
     3841 tgaaacgcct tgccgacgag ttcggactga cccacgaaac catcgcccaa gccgtcggta
     3901 aaagccgaag cgcgatttcc aacagcctgc gccttttaag cctgcccgaa cccgtgcagg
     3961 aaatgcttta ccaacgccgc ctcgaaatgg ggcacgcccg cgcactgctg accctgcccg
     4021 tcgtcgaaca gctcgaattg gcgcaaaagg ccgtcaaaaa cggctggtcg gtgcgcgaag
     4081 tcgaacgccg cagccaagcc gcccttcaaa acaaacgtcc cgagcccaaa aagactgccg
     4141 ccgccgacat cggccgcctg aatgatttgc tgactgaaaa actgggtgtc aacgccgaaa
     4201 tcaaaaccgc caaccataaa aaaggcaaaa tcatcctgca tttcgacacg ctcgaaacct
     4261 tcgaccatat cttgaagcag ttgggcatag attaccgacc ttaattttgc gggatatacc
     4321 gtctgaaata tagagaatag ctttccagat tttaagtggg aaatataatt ctattgacat
     4381 ttttctgctt cacgtaagaa tcgttttcct gttttcattt ttaattttcg aggaaattat
     4441 gaacacacgc atcatcgttt cggctgcgtt cgttgcgttg gcattagcag gttgcggctc
     4501 aatcaataat gtaaccgttt ccgaccagaa acttcaggaa cgtgccgcgt ttgccttggg
     4561 cgtcagccca aatgccgtaa aaatcagcaa ccgcaacaat gaaggcatac gcatcaactt
     4621 taccgcaact gtgggtaagc gcgtgagcca atgctatgtt accagtgtaa tcagcacaat
     4681 cggcgttacc acttccgatg caatttgttt gggaggcgga acgcacaaag gcaaaagtca
     4741 atgcaatgct ttgcttaaag cggcaggccg ttgctaatcc tttattcgga aaaggtcgtc
     4801 tgaaaatatt ttcagacgac cttttattta ttgagcaaat tccccaaact gccgacaatc
     4861 cgaccgataa actgctacaa tttcgcgttg attttttatt atttccacgt aaagcaaaca
     4921 aatcacgtaa gaacattaaa aatgtaaaga aattgcggac aaactcctta atatcctgta
     4981 attatatgta atgtgtgtta gaatgacacc tgtcgccgtt attcaggcgg cgagcgcggc
     5041 ggattgggat acgttgtttc acaaagatta actgtcgctt gacgtaataa ggcttctaaa
     5101 ttatattagc cgcgccccaa atccgaggcg gaaacattcc ctttatgaag cagattatca
     5161 tcctacaatc cgccgtattg tccatatgcg ccgcagttgc ctttgctgta tggggttttg
     5221 ccggattcct ttcggcggtc ggtggcggtt tgtcctacct gcttcccacg tttgttgcag
     5281 ttttactttt aaaacttttc aggggaaacc ccttcctgca aagcaggatg ttcgtcttcg
     5341 gagagatttt aaaagtagtg ctgtcgctgt tgtccatgct cgccgtattt gctgtatggc
     5401 atcaatcgtt ggtgttcgcc ccgtttctga tggggctgct cggtgtcagc catttggttt
     5461 ttttagtatt gttgagagtg aaagattatg gcaggtgaaa ccattaccgc tgccgactac
     5521 atcaagcacc acttgcagag cttgaccagt ttgtcggatg ttactcaggg tcagggactg
     5581 aaaaacattg ctgatttttc gtttattaac cttgatgccg tcttttttgc cgtcctgttg
     5641 ggcgtaatcg gcagcttcct gttgtggcgc ggtgcgaaaa aagcaacggc aggcgttccc
     5701 ggacgtttcc aggctgccgt ggaaatcttg tttgagtttg tggacgatat gtgtaagagc
     5761 atcattcaca acgaaaagtc tagaaaagcc gtcgcgccgt tgggtctgac gctgtttgtc
     5821 tggattttcc tgatgaacgc gatggatatg ctgccggtcg atttgctgcc gatggtatgg
     5881 cagggcatta ccggcaacca tcacgccctg ttgcgcgtcg taccgactgc cgatttgaac
     5941 accactttgg cactcgctgt cggcgtgttg ctgatttgta tctattacaa catcaaaatc
     6001 aaaggattgg gtggctggtt tcacgagttg ttcagcgcgc cgttcggtgc aaaactcgca
     6061 cctgcgaact ttctgttgaa tttggtcgag tttctttcta aaaccgtatc ccacggtatg
     6121 cggttgttcg gcaatatgta tgcgggcgag ttggtattct tgctgatagc cttgcttggc
     6181 ggtgcttggg cggcttccgg cagcgtcgaa gtcatggatc cgattctgtt tgtattccac
     6241 attattgccg gtttggcatg ggcgattttc catattttgg tgattaccct gcaggcgttt
     6301 attttcatgg cgttggcgtt cgtttatatc ggacaggcac atgatgcgca ctgattttcc
     6361 taagtagttt gtttttgtag tttaaccttt gtttctttaa ggagtttaaa atgggtttga
     6421 ttgctatcgc atgtggtttg atcgttgcat tgggtgcatt gggtgcctct atcggtatcg
     6481 caatggtcgg ttctaaatat ttggagtctt ctgcccgtca gcccgaactg atcggtccgc
     6541 tgcaaaccaa actattcctg attgccggtt tgattgatgc cgcattcttg attggtgtcg
     6601 ccattgcact gctgttcgcc ttcgttaacc cgtttgcagg tgcataatca ggacggattc
     6661 gtccgttccg tacaggcgca gcctgtttga ttaaccccag tacgaaggtt aagtaacgtg
     6721 aatatcaatg caacattatt cgctcaaatc atcgtctttt tcggtttggt atggtttacc
     6781 atgaaattcg tgtggccgcc gattgcaaaa gctttggatg agcgtgccgc aaaagtcgcc
     6841 gaaggcttgg ctgccgccga gcgtggtaaa agcgattttg agcaggctga aaaaaaggtt
     6901 gcagaactct tggcagaagg gcgtaatcag gtttccgaaa tggttgccaa cgccgaaaaa
     6961 cgtgccgcca aaattgtcga agaagccaaa gaacaggctt cttccgaggc ggcgcgcatt
     7021 gcagctcagg caaaggccga tgtggagcag gaattgttcc gcgcacgcga atccctgcgc
     7081 gagcaggttg ccgtgttggc tgtcaaaggt gccgaatcta ttttgcgcag cgaagtcgat
     7141 gcttccaaac acgcaaaact gctcgatacc ctgaaacagg agctgtaatc ttatggcaga
     7201 gttcgcaacg attgccagac cttatgcaaa ggcattgttc ggtctggctc aggaaaaaaa
     7261 ccaaattgag tcttggttgg gcggactgga aaaacttgcg gcggttgttc aggaagggaa
     7321 gatggcttca ttgattgacc gtcctgaaac gaatgcttca gaaaaagcag atatcctcat
     7381 cgatttggtc ggtttgaaag acaaggagtt gaaaaacttt gttatcgtct tggccgggca
     7441 gaaacgtttg tcgatattgc cggaagtata tgctcaatat caagacttga ccttatcatt
     7501 caaccatatc aaatctgccg tcatttacag tgcctatccg ttgaccgaca aacaggtcgg
     7561 cgagttggcg caaatgctga ataagcgttt cgacagcgag ctgaaaatct ctgtcgaaat
     7621 cgaaccggag ctgattggcg gcataaaagt tgaagtgggt gatcaggttt tggatttgtc
     7681 tgtacaaggc aaattgagtg ccttgtacac gactatgacg aattaggaga gttttcatgc
     7741 agcttaatcc tgctgaaatt agcgatttga ttaaagccaa gatcgaaaat ctgtctgtaa
     7801 atgccgaagt gcgtactcgt ggtacggtga tttcggtaac tgacggtatc gttcgcatcc
     7861 atggtttgtc agatgcaatg caaggtgaga tgctcgaatt cccgggtaac actttcggct
     7921 tggcgatgaa cctggagcgc gactccgtcg gcgccgtagt gttgggcgag tacgaacata
     7981 ttaaagaagg cgacacggtt acctgtaccg gtcgtatctt ggaagtgccg gtcggacgcg
     8041 aactggtcgg acgcgtcgtt gatgcattgg gtcggcctat cgacggcaaa ggcccgatta
     8101 atacaacttt gaccgcccct atcgaaaaaa tcgcaccggg cgtgattgcg cgcaaatcgg
     8161 ttgaccagcc gatgcaaacc ggtctgaagg cgattgactc tatggttcct gtcggtcgcg
     8221 gtcagcgtga gttgattatt ggcgaccgtc aaaccggtaa aacggccgta gcattggatg
     8281 ccatcgtcaa ccaaaaaggt acgggtgtta tctgtatcta tgtcgctatc ggtcaaaaag
     8341 catcttctat tgccaacgtg gtccgcaaat tggaagagca tggcgcgatg gagcatacca
     8401 tcgtggttgc tgcaactgca tctgaagcgg cggcattgca atatatcgca ccttactccg
     8461 gttgtacgat gggtgaattc ttccgcgatc gtggcgaaga tgccttgatt gtttatgacg
     8521 atttgtccaa acaggctgtg gcttaccgtc aaatttccct gcttttgcgc cgtccgcccg
     8581 gccgcgaagc ctatcccggc gatgtgttct acctgcactc ccgtctgttg gaacgtgcag
     8641 cacgtgtcaa tgaacacgaa gtggagaaat tgaccaacgg cgaagtaaaa ggcaaaaccg
     8701 gttctctgac cgcgttgccg attatcgaaa cccaagcggg cgacgtatct gctttcgttc
     8761 cgactaacgt catttcgatt accgacggtc agattttctt ggaaaccgac ctcttcaacg
     8821 ccggtatccg tcctgcaatc aatgccggta tttccgtatc ccgcgtaggc ggtgctgcac
     8881 aaaccaaagt gattaaaaag ctgggtggcg gtatccgttt ggcgttggcg caatatcgtg
     8941 aattggcggc gttctcgcaa tttgcatccg atttggatga agctacgcgc aaacaattgg
     9001 agcatggcga agtcgtaacc gaactgatga aacagaaaca gttcagtacg ttgaatacgg
     9061 ctgaaatggc tttgaccctt tgggcaatca acaacggttc gtattctgat gttccggttg
     9121 ccaaagctct ggcttttgaa tctgaatttt tgagctttgt ccgtacccaa catccggaag
     9181 ttttggaagc cgtcaatgct tcaggtgcaa tgtccgacga gagcgagaaa acgcttgaag
     9241 cagccatgaa atccttcaaa tcttcttacg cctatcaggc ataaggcttg aagtgaaagg
     9301 agtctgaaat ggcagtagga aaagagattc tcaccaaaat ccgcagtgtt cagaataccc
     9361 aaaagatcac taaagcgatg cagatggtgt caacctctaa aatgcggaag actcaggaac
     9421 ggatgcgttt ggcgcgtccg tatgccgaaa aagtgcgtat ggtgatgagc catcttgcgc
     9481 aaaccaatac cgatcatggt attccgttac tggaatctca tcgggaaatc agacgtgtcg
     9541 gttttatttt gattacgtct gataagggtt tgtgtggcgg tttgaacgcc aacgtgctga
     9601 aaaagttttt ggcacaagtt caagagtatc ggaatcaagg tattgaagag gaaatcgtat
     9661 gccttggcag taaaggtctg atggcgtgtc agagcattgg tctgaatgtg gttgccagtg
     9721 ccgtaaattt gggcgatacc ccaaaaatgg aaatgctgct cggacctttg acagaactct
     9781 tccaacggta tgagaaacat gaaattgaca gaatccatct ggtgtattcg ggttttgtca
     9841 ataccatgcg tcaagaaccg agaatggaag tattgctgcc tatcggtgag aacgtgattg
     9901 gcgattcagc tcccaaatca ccgttcagct gggaataccg ctacgaaccg actgcacttg
     9961 cagtgttgga atatctggtt cgccgctatt tagagtctgt ggtttatcag gcgttgagcg
    10021 acaatatggc atccgaacag gcagcccgca tggttgccat gaaagctgca acagacaatg
    10081 caggcaatgc catcaaagag ttgcgtttgg tatataacaa atcgcgtcaa gctgcgatta
    10141 ccacggaatt gtcagaaatt gtagcaggtg cggcggctgt ctgatgccgt ctgaatcctg
    10201 tacggaaatt aggatacgat aatgagccaa ggcaaaatcg tacaaattat cggtgcggtt
    10261 gttgacgtgg aatttccacg cgacatgatt ccgcgcgttt acgacgcttt gaaattagac
    10321 gaaaacggtc tgactttgga agtccaacag cttttgggcg acggcgtagt ccgtaccatt
    10381 gcgatgggca gctcggacgg tttgaaacgc ggcatgactg tgaacaatac cggtgcgccc
    10441 attactgtgc cggtaggtaa aggtacgttg ggacgcattg tcgatgtatt gggaacgcct
    10501 gttgacgaag caggtccgat cgataccgac aagagccgtg ccatccacca agccgcccct
    10561 aagtttgacg aactgtcttc cacaaccgaa ttgctcgaaa cgggcattaa agtgattgac
    10621 ttgctgtgtc cgtttgccaa aggcggtaaa gtaggtctgt tcggcggtgc cggtgtgggc
    10681 aaaaccgtga acatgatgga attgatcaac aacatcgcca aagcgcacag cggcttgtcc
    10741 gtgttcgcag gcgtgggtga gcgtacccgc gaaggtaacg acttctacca cgagatgaaa
    10801 gattccaacg tattggataa agtggcaatg gtttacggtc agatgaacga acctccgggc
    10861 aaccgtttgc gcgtcgcatt gaccggtttg accatggcgg aatacttccg tgacgaaaaa
    10921 gacgaaaacg gcaaaggccg cgacgtattg ttcttcgtgg acaacatcta ccgttacact
    10981 ctggccggta ccgaagtatc cgcattgttg ggccgtatgc cgtctgcagt gggctaccaa
    11041 ccgacattgg cagaagaaat gggtcgtttg caggagcgta ttacctctac ccaaaccggt
    11101 tctattactt ccatccaagc cgtatatgta cctgcggacg atttgaccga cccgtctccg
    11161 gcaacaacgt tcgcccactt ggacgcaacc gtcgtattga gccgcgatat tgcctctttg
    11221 ggtatttacc cggcagtcga tccgcttgat tccacttcgc gccaattgga tccgatggtc
    11281 ttgggtcaag aacactacga cgtggcgcgc ggcgtacagt ccaccctgca gaaatacaaa
    11341 gaattgcgcg acatcatcgc catcttgggt atggacgaat tgtctgatga agacaaactg
    11401 accgtaatgc gtgcgcgtaa aatccaacgc ttcctgtccc aaccgttcca cgttgccgaa
    11461 gtgtttacag gttctccggg taaatatgtc gccctgcgcg ataccattgc cggcttcaaa
    11521 gccatcttga acggcgaata cgatcatctg cccgagcagg cattctatat ggtcggcagc
    11581 atcgaagaag cggttgagaa agcgaaaacc ttaaactgag gaggtcggca tgagcatcat
    11641 gcaagttgag gtggtcagta gcgagcaaaa catctattcg ggcgaagcca gctttgtagt
    11701 ggttccgacc gttcaaggtg agcttggtat ttacccgcga cacgagccga ttatgagttt
    11761 ggtgcggccg ggggctttgc gtttgaccgt tccgggcgag gataaagagg ttttggttgc
    11821 tgtttccggc ggtgttttgg aagtacagcc tgataaagta actgtcttgg cggatgttgc
    11881 cgtccgcagt gcggagatgg atcaggcacg tgcggaagag gcgaaaaaag ccgcagaagc
    11941 gggcatttcc caagctaaag acgataaggc tttggcggaa gcacataaag cattggctgc
    12001 tgcgattgca cagctcaaaa ctttggacta tatccgttcg cacaagaaat aaacttgttt
    12061 ggtttggaaa agcacgaccg tttggtcgtg ctttttattt tccggattta aattaaagac
    12121 tgtgtttgga tatggcgttg gcaggcatag cctgctttaa ggtattcaat agggcagcct
    12181 gccgcctaac cgaaa
//
LOCUS       DAWFBO010000046        12148 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFBO010000046 DAWFBO010000000
VERSION     DAWFBO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.046"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            141..1538
                     /gene="aspA"
                     /locus_tag="WGO87_000925"
     CDS             141..1538
                     /gene="aspA"
                     /locus_tag="WGO87_000925"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0392303.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1610..2485)
                     /locus_tag="WGO87_000926"
     CDS             complement(1610..2485)
                     /locus_tag="WGO87_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0392304.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2487..3209)
                     /locus_tag="WGO87_000927"
     CDS             complement(2487..3209)
                     /locus_tag="WGO87_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0392305.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQQAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYLLGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNDGRAVAPEKWI"
     gene            complement(3232..3711)
                     /gene="ybaK"
                     /locus_tag="WGO87_000928"
     CDS             complement(3232..3711)
                     /gene="ybaK"
                     /locus_tag="WGO87_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0392306.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFSRLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3786..4148
                     /locus_tag="WGO87_000929"
     CDS             3786..4148
                     /locus_tag="WGO87_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0392307.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWCVEIKITAVKRDAATA"
     gene            4183..5046
                     /locus_tag="WGO87_000930"
     CDS             4183..5046
                     /locus_tag="WGO87_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0392308.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLAADGEEAGIVLDSVKDSESFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5144..6103
                     /gene="ttcA"
                     /locus_tag="WGO87_000931"
     CDS             5144..6103
                     /gene="ttcA"
                     /locus_tag="WGO87_000931"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0392309.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(6237..6791)
                     /locus_tag="WGO87_000932"
     CDS             complement(6237..6791)
                     /locus_tag="WGO87_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0392310.1"
                     /translation="MKPKFKTVLTALFLAASLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNIPVQPVKIRRVVAE"
     gene            complement(6856..7782)
                     /locus_tag="WGO87_000933"
     CDS             complement(6856..7782)
                     /locus_tag="WGO87_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0392311.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMEQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCPPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKFTDGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(7770..8246)
                     /locus_tag="WGO87_000934"
     CDS             complement(7770..8246)
                     /locus_tag="WGO87_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0392312.1"
                     /translation="MKTNFKQKIIDQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGAVAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            complement(8317..8772)
                     /locus_tag="WGO87_000935"
     CDS             complement(8317..8772)
                     /locus_tag="WGO87_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0392313.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKICQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETYRLADAGCRALLEKISK"
     gene            complement(8897..10012)
                     /locus_tag="WGO87_000936"
     CDS             complement(8897..10012)
                     /locus_tag="WGO87_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0392314.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(10126..11223)
                     /locus_tag="WGO87_000937"
     CDS             complement(10126..11223)
                     /locus_tag="WGO87_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0392315.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(11305..>12148)
                     /locus_tag="WGO87_000938"
     CDS             complement(11305..>12148)
                     /locus_tag="WGO87_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0392316.1"
                     /translation="AFNDPIYVNGFIEWANRHRLAYIGDADLHASFVSWMAEHTRERI
                     LALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESVAVEVIESLNFRPARG
                     ETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLTFDRMKINSQLLLQTIL
                     GRFSVSSDNAGKQFFEDHKTYVPARFTDYAAAFVEHGAEAFVRPANRYNESTPSFGYG
                     HLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEVYVEEILADLADRHFL
                     VSAD"
BASE COUNT         2951 a         3345 c         2973 g         2879 t
ORIGIN      
        1 cccgaaaaca acgtttcccg ttttcagacg gcatacgata tggacaattt catccggccg
       61 gtatagtgtt cgtcctgcgg cggcatcttt ccgatggcgc attttttcaa tattaattat
      121 ccgacttaca aaggtaaaac atgactgtcc gtatcgaaca cgatttattg ggcgaccgcg
      181 agattcccgc cgaagtgtat tggggcatcc acactctgcg cgccattgaa aactttaaaa
      241 tctccacaca aaaaatttcc gacgtgccgc agtttgtccg cagtatggtg atggtgaaaa
      301 aagcaaccgc gcaggcaaac ggcgaattgg gtgcagtaaa acccgaaatc gccgccgcca
      361 ttgaaaaggc ttgcgacgaa gttctgctga acaaccgctg cctcgaccaa ttcccctccg
      421 acgtgtatca gggcggtgcg ggaacttcgg ttaatatgaa caccaacgaa gtcatcgcca
      481 acctcgcatt ggaagccttg ggatacgaaa aaggccgcta cgacatcgtc aatccgatgg
      541 atcacgtcaa cgccagccaa tccaccaacg atgcctaccc taccggcttc cgccttgccg
      601 tgtattacag catcggcgaa ttgctcgaca aactgactgt cctgaaaaac gcctttgccg
      661 ccaaagccga agaatttaaa gacgttttga aaatgggccg cacccagctt caagatgccg
      721 tgccgatgac tgccggccaa gaattccaat ctttccaagt gttgttggaa gaagaaatcc
      781 tcaacctcga ccgcacccgc caactgctgt tggaagtcaa tttgggcgca acggcaatcg
      841 gcacgggcgt gaacacgccc aaaggctacg ccgaactggt ggtcaaaaaa ctctccgaag
      901 tcagcggctt gccttgcaaa ctgaccgaaa acctgattga ggcgacctcc gactgcggcg
      961 catatgtgat ggtacacggc gcattgaagc gcacggcggt caaactctcc aaaatctgca
     1021 acgacttgcg cctgctctct tccggcccgc gtgccggctt gaaagaaatc aacctgcccg
     1081 aattgcaggc cggttcttcc ataatgcccg ccaaagtcaa tcccgtgatt cccgaagtgg
     1141 tcaaccaagt ctgcttcaaa gtcatcggca acgacacgac gattaccttc gccgccgaag
     1201 ccgggcaact gcaattaaac gtgatggagc cggtcatcgc ccaatgtatg tttgaaacca
     1261 tttccctctt gggcaatgcc gcagtcaacc tgtccgacaa atgcgtcaaa ggcattacgg
     1321 tcaaccgcga aatctgcgaa cgttacgttt tcaactccat cggtttggtg acttatctga
     1381 atccgtatat cggacaccac aacggcgatt tggtcggcaa aatctgcgcc caaaccggca
     1441 aaggcgtgcg cgaggttgtc ttggaacgcg ggctgttgag cgaagaagaa ctcaaccgca
     1501 tcctctctcc cgagaacctg atgaaccccc atctgtaacg ttgcagacaa atgccgtctg
     1561 aagcagggat gctgcttcag acggcatttt gttatatcaa tcagccggat tatatgccga
     1621 acagtgcctt gacctccgcc caataaaaca ggcaggcgat ggaacacagt gccaacacga
     1681 ttcccgccgt attgattgtg ttgatttttt ccttaaaaaa cagtgcgccc gaaagcgtcc
     1741 ccaaaacaat cacgccgata ttcataccgg caaaaaccag cgtgggattg tccttcatca
     1801 tttggtgcgc ggtgatgtag gttacgatgt tcatgaagtt caaaccgccc aagagtatgc
     1861 cgccgaccac actctcaacg cgccatctga ccgatttggc aaacaggcag gcaaacatca
     1921 gcacacccgc cagcacaaat gcaaccagca ggttgcccgc aaatgccgtt ccgcttttgg
     1981 caagctgttt gaacaggata tcgataatgc cgtaacctgc ccacacgccc agcagcaatg
     2041 ccgcctgccg ccacgcgctt cctgattttt tgccaccgct gtgtttccaa agcaggcaga
     2101 acagtgcggc aaatgcgagg cacagcccga ttagtctgcc ttcgctgagt ttttcgccaa
     2161 acagcgtcaa ggcggcaaca atcggcaaaa acagcgacaa acgctgcgcc gcgtcggatt
     2221 tgacgatacc ggcggattct acggatttgc ccataatgac aaatacggac ggcagcagca
     2281 cgcctaatgc ggcaaacagc ggccacgtcg gcaaaaatgc gccgatattg ccgacatccg
     2341 gcttcaatac cagcaaagtc agtatgaccg cgaccacata attgacggcg accgcctgcg
     2401 cgatgtcgat ttttttcttc cttgccattt tcagcaaaat ggaaaccgac acgctgaaca
     2461 cgatgctgat taacaggtaa gccatactat atccatttct cgggtgcaac cgccctgccg
     2521 tcgttgaagc ggataaagcc ggcgatgatg cggtagaaat accagacgtt cgttgcaata
     2581 ataatcagca cgccgatacc caaaagggca gtcagcgcgc ccaaaagata aagccaaaat
     2641 gtgcgccaaa aggttttaat caggtattcg gtatgtgcgg catagacaat gctgtccaaa
     2701 ctgttccgtt tcatataggc gagaatcacg ccgactatgg gcatataggc gaaaatcacg
     2761 ccgactatgg gcatgaacag catgattgca cccaaaccga acaggatgta ggcggcgagg
     2821 acatagttgc gcccgcttcc gccgccggac ggttcgtaag catggcgcga acgtgaggcc
     2881 tgccgttcaa acgcctgtct gaacgcctgt tcgcggcgca tctgttcgcg gtaaaactgc
     2941 tccctgcgcg cctgttcgcg gcggaatgcc tcttcctgcc gcccgcgttc ctcatgccgt
     3001 ctgaaggacg cgtcgtattg catccgtttc gacaggtcgg aaagcgtatc gtaagcctgt
     3061 tggatttctt tgaacttttc ttccgcctgc ggattgcccg gattgcggtc gggatggtat
     3121 ttcatcgcca gcttgcggta ggcgcgtttg atttcgtccg ctcccgcctg cggcgacacg
     3181 cccaatacgg catacaaatc tttgtccata ttgttccttt atactttccc gtcaaaccgc
     3241 cgcctgtatt gttttcgggt tcaaaatatt taaatctccg ggacggatgc cgataatgaa
     3301 cccgcgtttt ccgccgttga tatagatggt ttccaaatcc atcaccgact gttcgacgta
     3361 aatatccaac tttgtccgga tgccgaacgg cgttgtgcct ccgaccagat agcccgtcca
     3421 cttgtttgcc tgtgcgggcg tagcgggttc gatgtgtttc gcacccaaat gccgagccag
     3481 attgcgcgtg gaaatctgtt tgtcgccgtg catcaagaca atcagcccct taccgttttc
     3541 atcttgcaaa acaatggttt taatgaccaa gtgttcgtct ttgccgaaca gtcgggaaaa
     3601 ctgcgccgtg ccgccgtgtt cctcataggc ataggtaaaa ggttcaaatt cgatgccgtt
     3661 ttcacgcaaa acgcgcacgg caggtgttat cggataagtg tgtttactca tttttcgtca
     3721 tgcaaaacat ggaaatcggc caagcgcaat gttaccatat atccccttta tcgaaggaaa
     3781 ccgatatgga tatccgatat ttcggcacaa cgccccgcta ttccgaagcc gtcggcgcaa
     3841 acggcttgat tttcctctcc ggcatggttc ccgaaaacgg tgaaacggct gccgaacaga
     3901 ctgccgacgt gcttgcccaa atcgaccgct ggctggcgga atgcggctcg gacaaagcgc
     3961 acgttttgga tgcggtcatc tatctgcgcg atatgggcga ctatgcggaa atgaacggcg
     4021 tgtgggacgc ttgggttgcc gccggcagaa cgcccgcccg cgcctgcgtg gaagctcggc
     4081 tcgcccgtcc ggagtggtgc gtcgaaatca aaataaccgc cgtgaagaga gacgcggcaa
     4141 ctgcataaac agatttcctt tatatttcag agtacgcaaa tgatgaaaac cttactgccc
     4201 tttttcggcg tggtgcgcgt cagcggcgaa gaccgccaaa ccttcctgca cggacagctt
     4261 tccaacgaca tcaaccacct tcagaccggg caggcgtgtt atgcgactta caacacgccc
     4321 aaaggccgcg tcatcgccaa tatgattgtc gtcaaccgag gcggcgattt gctgctgatt
     4381 atggcgcaag acctgcttga agcaacggtc aaacgtttgc ggatgtttgt gttacgcgcc
     4441 aaagccgttt ttgaaatcct tgaagattac gccgtcggcg cggaattaga agcatccgcc
     4501 gaaccgcttg ccgcgcaaga acccagcctc gcctttaccg ccgaatgcgg ttcagacggc
     4561 atctgttccg tcgtcctgcc acatcggggc attttgcaca tcgcccccaa aaacgccttg
     4621 ccgccttatg atgccgccgc cgaaaacgcg tggcggctgc acgaaatccg cagcggttac
     4681 ccgtggatat gcgcggctac caaagaaact gccgttgccc aaatgctcaa ccagcacatc
     4741 atcggcggcg tacacttcaa aaaaggctgc tatcccggtc aggaaatcat tgcccgtgcc
     4801 cagtatcgcg gtcaggtcaa acgcggcttg gcggtattgt cgggaaattc ggcagccgaa
     4861 gcaggcatcc tgctggcggc agacggcgaa gaggccggca tcgtgctcga cagcgttaaa
     4921 gattcggaaa gctttaccgc ccttgccgtg attaaattct ccgccgcaca aaaagaactg
     4981 accgccccaa acggcagtat cttcaaagcg gttcatccct tctttaaaac cgaaaatgca
     5041 gaataatccc tctaatgccg tctgaacctt tttcagacgg cattttaaaa ccgcccctgt
     5101 tttcagttac aatgccgcat ccccaatttt tcaaaccacc gccatgtcca aaaaaaccaa
     5161 acaagaactg gaaaacaaca aactcagcaa acgcctgcgc cacgccgtcg gcgacgcgat
     5221 taacgatttc aacatgatcg agccgggtga caaaatcatg gtctgcctct ccggcggcaa
     5281 agacagctac gccctgttag acatcctgcg ccggcttcaa gccagcgcgc cgattgattt
     5341 cgaactggtc gccgtcaatc tcgaccaaaa gcagccgggc ttccccgaag aagtattgcc
     5401 gacctatctc gaaagcatcg gcgttcccta caaaatcgtt gaagaagaca cctactccac
     5461 cgtcaaacgc gtattagacg aaggcaaaac gacttgttcg ctgtgcagcc gcctgcgccg
     5521 cggcatcctc taccgcactg caaaagaatt gggctgcacc aaaatcgcct tgggacacca
     5581 ccgcgacgac atcctcgcca ccttgttttt aaatatgttt tacggcggca aactcaaagc
     5641 catgccgccc aagctggtga gcgacaacgg cgaacacatc gtcatccgtc cgctggcgta
     5701 tgtaaaagaa aaagacttaa tcaaatacgc cgaattaaaa caattcccca tcatcccgtg
     5761 caacctctgc ggctcgcaac ccaacctgca acgccaagtc atcggcgata tgctgcgcga
     5821 ttgggacaaa cgcttccccg gccgcatcga atcgatgttc tccgccctgc aaaacgtcgt
     5881 cccttcccac cttgccgata ccgaactctt cgactttgtc ggattggaac gcggtcaaac
     5941 cctgaaacac ggcggcgatt tggcgtttga cagcgaaaaa atgcccgaac gtttttcaga
     6001 cggcagcgaa gaagacgaaa gcgaaatcaa aatcgagccg caaaaagccg aacgcaaagt
     6061 catcaacatt ctggcgaaca agccgaaaac ctgcggcgcg taaacctcca ccgtcaaaaa
     6121 tgccgtctga agcccgtttg gcgtttcaga cggcatttta aaatcccgaa gtagacactt
     6181 ttcaacccca aacgcctgct ttttccgttt cagctttctg aaggtttccc gttatattac
     6241 tcggcaacaa cgcggcgaat tttgacgggc tgtacgggta tgttttgata aaagccgcgc
     6301 gtggcggttt tgacgcgggc gattttggaa acggtgttca tgccgctttc gaccctgccg
     6361 aaaacggtat agccgtattg tccgtttttg tagttgagtg aatcattgtc ggccagattg
     6421 ataaagaatt ggctggtggc ggaatccgga tcggccgtcc gcgccatggc gatggtaccg
     6481 gcagtgtttt tcaagccgtt gccggattcg ttggcaacgg ccttatcggt tgccttttgt
     6541 accaagtctt cggtaaatcc gccgccctgg ataacgaaac cgtcgataac gcggtgaaaa
     6601 acggtgtcgt cgtaaaagcc ttttcgagca tagcgcacga aattagcaac ggttttgggg
     6661 gctttggact cgtccaaaac caaacggata ttgcccatgt cggtttccat caaaacatgg
     6721 gttgccgcca tagacggcag ggaagccgcc aaaaacagcg cggttaaaac ggttttgaat
     6781 ttgggtttca tcccgtcctc ctcagacctt cagacggcat tttcatttca tatgccgtct
     6841 gaaagcgcgt gaacactatt ccaatgcgtc tttaagtttt tgttcgatta aatccgcatc
     6901 aaacgatttg gcaatcaatt caaaacggga atcacgccgc caagacactt ggttggcgcc
     6961 ccattgcccg tccacccagt tgagccatac ccatgtaccg agcacttgga acacgccttt
     7021 ggcgcgaaca agaccgtctg tgaatttggg caagtcgttg aagaagttgg tcagcttttc
     7081 gccgtcgaaa tcccgtccgg cggggaatgt gaaaccttgc gactggaatc ccatggtgtt
     7141 gtccggcagg gctttgaggc ggtagcgtga tttttcgatg acgggaatat cgagccattg
     7201 gatgtcgagt tgtgcgtttt ggacttcgac cactttggct ttgggcggga acagttttgc
     7261 cgctttgtcg tgaaattcgg caagctgttc gggggggcat aaatcggttt tgctggcgac
     7321 caatacgtcg caaataccga tttggtcttt atataaggct tgttgcgcgt aatcgggatt
     7381 aatgaattgg cgcggatcga cgacggtaaa gactgcgccg atttccaaaa ggctgtccag
     7441 cggtttggtt ttcagttcat caataacact ggcggcgtgc gccagtccgc ttgcctcaat
     7501 catcaggcgg tcgggcttgg cgtcgcgcag cattttctgt acggttacgc ccatttgcgg
     7561 accggcggtg caacacaaac agccgccggc gatttctgcc acggggatgc cgttgtcgct
     7621 caataccgcg ccgtcaatgc cgatttcgcc gaactcgttg acgatgatga cccatttctc
     7681 gttcgggtct ttctgctcca tcaggctttt aagcgcggtg gttttgcctg ttcccagaaa
     7741 acctgaaatc aggtggactt tggttttttt catttctgac attttttaca gattccagtt
     7801 aaaacaacgt gttcttcttt cagcgcgaag ccgctttcgg caacgcctgc gcgcaatgcc
     7861 gcccactcgt ggctcagggt ttgctcgtcc gccgtgccgc attcggtgca gaccaaaata
     7921 aacgcgctgt ggtgcgcttc ggcttcttcg tggtcgtggc aatggtcgtt gcactcgtgc
     7981 tgcgcgtggc tgcacaaaat atagccgttg accgccgcca ctttgtgcaa aacgccctgc
     8041 tccgcccaaa aatcaagggc gcggtaggcg gtcggcggcg caaccgcgcc ctcgctttgc
     8101 tgctgcatct gcgacaagac gttgtaggct ttaatcacgc cgctttgctg caagacaata
     8161 tctaatacct gctcgcgcaa agcggttacc tgcaagcctt cgctgcgtgc ctgatcgatg
     8221 attttttgct tgaaatttgt tttcataaat tccctgttta tgccgtctga acgaccgata
     8281 cggcaggagg cggttttata tttgtattca attactttat ttggaaatct tttccaacaa
     8341 tgcccgacag cccgcatccg caagcctgta tgtttcttca aaatcgcccg tgtaccacgg
     8401 atcggggaca tggtcgtaac cgctttcggg tatcaggtcg gtcagcttga atattttttc
     8461 cggccgcctg ccgaaggttc tttccaattc ggacaaattc ttgccgtcca tcgcgatgat
     8521 gcagtcaaac gccgccgcat cgctttggca gattttgcgg ctggtaaagc ctgaagcatc
     8581 gataccgtgt tttttcaata tctttgccgt ctcgcggtgc atatcttcgc cgtcgtgcca
     8641 gcccgatgtc cccgcgctgt ccgcttcaag gggaatgccc gcttcggcgg cgcggcggcg
     8701 caaaatgtat tccgccatcg gcgaacggca gatgttgccg aggcagacaa aaaggatttt
     8761 cggttttttc atatcccctc cctgttccgg cgcgatgccg tctgaagcgg aaaccctttc
     8821 agacggcatc ggtcggttaa tcgtaaccgc ataaggcaga accagccaaa acatccatct
     8881 ggaagaaatg gtttggctaa atcttaggca tatttaataa gtgtccaata ttagaagccg
     8941 tatgctccaa atagagactg gcatttttca aactatcttc taaaggttca cttttctcca
     9001 aaatagaaaa ggcagcttgg atattttcaa atggcaggga aggcaaatct tcaacgagac
     9061 tgccacaaat agcgacaaca ggaactccga caggggttct ttttgctaca ccaataggcg
     9121 ctttccctgc taaactttga cgatctagtc ttccttcacc aatgataacc aagtcaacat
     9181 ctgacacttt cttatcaaag tcaatcaagt ccaggcaggt gtcaattcca gatacgatac
     9241 ttgcctgagc aaaggcgcac aaaccaccag cgatgcctcc accagctcct gctcctttaa
     9301 gttttaatgt tgcaggggag actttttcat aaaaatcttg tattgcctga tctacggcct
     9361 caaacatagt agaatccaac cctttttgct tgccaaacgt ataggtcgca ccttggtgtc
     9421 cacataaggg actcacaaca tctgctaaaa tacgaatgtg aacatcttca ggaatttcat
     9481 agcgattttc tgttgaaaca gaagctaggt ttagtaagga ttgaccgcaa acgggtaagg
     9541 tatttccatc ctcatcataa aattgataac ctaaaccagc agcaatccca atacctccat
     9601 cattactggc cgtaccgcca acgccaatat agatttcttt aatttcttga ctaatgaggt
     9661 ggcgaatcaa ttctccaata ccacgagttt ggatttgtaa tggatttcgt ttctctagcg
     9721 ggatttttcc aagaccaacc aaatcagcaa cttcgaatag ggctagttgt tctttttgaa
     9781 aatagcgcat gacttctttt tgtccaaaag gtcctgtcac ttgaagacat ttttcttcta
     9841 ggtcaagaga atgtcggata gcatctacag tgccttctcc cccatcaccg acaggacaga
     9901 ggagacattc cacatctgct atcgattgtt ggaagcctct ttttattgct tcagctacct
     9961 gttgagctgt caagctttcc ttaaacgaat ccggtgcaat tacaatcttc atatttataa
    10021 ttcatccttt cgtttcactc aaggcacaac acagaatgaa aaagtgttgt gctctttatt
    10081 ttgatttatt atataaatga gaaagcctat cactactaca aatcactatg cgctgaaaaa
    10141 cggattgtgc ccttcccgtt tcaatgcttc cgcatagctc gggatgcttt cctgttcgcc
    10201 caagggattg tgcaacaggt aaacgtgttc cacgcggcac tccgccatca ggtcgaggaa
    10261 attttgctgg atgacggcga tgttgtcggt aaaggaaagc tgccacgaaa acacctgcgg
    10321 cacgacttca aacgcgccgt cggtcgcatt aaacccgaat aaaacctgtc cgcccgcctt
    10381 tgccgcagcg accacgccga cgcgcggact ctgcatacgg acggcgcgta tcgcgtttgc
    10441 cgcaaacaca tccatcgcca tacgctggcg cgtgtggctg ccgtctgaaa gcgcgcgtac
    10501 cggagaatcc ggggaaagtg gattggcaaa cagcgtaaca ccttccgatg caagattttc
    10561 ctgccatacc tgcattaagg gcaggcctgc ctgaagaagg ttctgaccca atgccgtctg
    10621 aaccttgccg ctgccgaaac ccagcgcgta aacgacgtgg acggactgcc cttcgggcaa
    10681 cagcaaaggg cgcggggcga aacggcgtaa gtgttgcgcc gccgcttcgg tattttgttt
    10741 ggcacgccac cactcatccg gcgcgaccgc actcaaatcg gacgaatgca caagataagg
    10801 cagccattgt gtctcttgcg tcaacgcgcg caggtgcgga tagttttgca ggcaggcaaa
    10861 caatgcctcc gtcggcagct tcatctcgat tgcccgttct ttcttgcagc cggacaccag
    10921 cacgaccggc agggcgaacc actgcacttc gccttctttc tcgcaatcga gtaccgcgtt
    10981 cacactggaa agcagcgcgg cataagttcc ggcatcgggc gacatcgtca acgcgaggga
    11041 aaggttgata tagtggtttt gctcaagcat ccccctgatt tcggtttgaa gttggccgga
    11101 cgagagtttg cgcgaagcct gggaagaatt atgcgccaac tggtaggcat tgagcagcag
    11161 gtggttctta atcggatttt ggggatacgg gcgcgtatcg ggcaaggtaa atgtctggtt
    11221 catatgtgtc gtgtaccgct tttcgtagtg tgtgtttttc ctgtttgaaa atatatcgga
    11281 cggattgccg tcgggacttg cccgtcaatc cgccgaaacg agaaaatgcc tgtctgccaa
    11341 gtctgccaat atttcttcca catacacttc ggcaggcgga tggaatgtca aaccgtcggg
    11401 cgtggtgctg acgatgttca agttttcggc aaccgtttca atcaatgcct gtttgctcgt
    11461 cggccgcgac aattgccgca taatgtacaa atgcccatat ccgaatgagg gggtgctttc
    11521 gttgtaacgg ttggcaggcc ggacaaacgc ttccgcgccg tgttccacaa aggcggcggc
    11581 ataatctgta aaacgcgccg gcacataggt tttatggtct tcaaaaaatt gtttgcccgc
    11641 attgtctgat gaaacagaaa aacgcccgag aatggtttgc aataaaagct gggaattgat
    11701 tttcatacgg tcgaatgtca aaccgggaaa tcggcgggcg agattttccg caacatcttc
    11761 cgttttaaac ggctctccgg ttttcatcac atcgcgtatg cccgaaagca gggtatcgtt
    11821 ttcatcaaag ttgattgttt cccctcttgc cgggcggaaa ttcaaacttt ctatcacttc
    11881 gacggcaacc gactcgtcac gcctgacagt atccccgact tcctcacggc ataaaagcga
    11941 acggcggaat tggcggtcgg ataaaatatc gctgtaaaat tctttggcaa tataatcgcc
    12001 ccctgccaat gccagaatcc gctcccgcgt atgctccgcc atccaagaaa caaaagacgc
    12061 gtgcaaatcg gcatccccga tatacgccag ccggtggcgg tttgcccatt cgatgaagcc
    12121 gttgacgtaa atcgggtcgt taaacgcc
//
LOCUS       DAWFBO010000047        12143 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFBO010000047 DAWFBO010000000
VERSION     DAWFBO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.047"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(46..975)
                     /locus_tag="WGO87_000939"
     CDS             complement(46..975)
                     /locus_tag="WGO87_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0392317.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1106..1855
                     /locus_tag="WGO87_000940"
     CDS             1106..1855
                     /locus_tag="WGO87_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0392318.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            1978..2559
                     /gene="petA"
                     /locus_tag="WGO87_000941"
     CDS             1978..2559
                     /gene="petA"
                     /locus_tag="WGO87_000941"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0392319.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2578..3927
                     /locus_tag="WGO87_000942"
     CDS             2578..3927
                     /locus_tag="WGO87_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0392320.1"
                     /translation="MANQTNSKAKALLGWVDARFPLSKMWKEHLSEYYAPKNFNFWYF
                     FGSLSMLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKLKDENGVPLDGIPFFPYYVVHDILAVTIFLIVFCAVMFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFAGTQVWGVIGMGA
                     AVVLIALLPWLDKGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITVVGAYLFATNI"
     gene            3942..4727
                     /locus_tag="WGO87_000943"
     CDS             3942..4727
                     /locus_tag="WGO87_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0392321.1"
                     /translation="MKQTLKNWFAALLLAVPMSAAVASGGGHYEKVDIDLRDQVSLQH
                     GAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDAA
                     KWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQG
                     VQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVNY
                     LVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLNKEFSKDIH"
     gene            5101..6885
                     /gene="rmuC"
                     /locus_tag="WGO87_000944"
     CDS             5101..6885
                     /gene="rmuC"
                     /locus_tag="WGO87_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0392322.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     QGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(7091..7723)
                     /locus_tag="WGO87_000945"
     CDS             complement(7091..7723)
                     /locus_tag="WGO87_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0392323.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7815..8639
                     /locus_tag="WGO87_000946"
     CDS             7815..8639
                     /locus_tag="WGO87_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0392324.1"
                     /translation="MIKKTIGGIIPIFMAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISAAVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRARTDR"
     gene            8708..9271
                     /locus_tag="WGO87_000947"
     CDS             8708..9271
                     /locus_tag="WGO87_000947"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0392325.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9352..9789
                     /locus_tag="WGO87_000948"
     CDS             9352..9789
                     /locus_tag="WGO87_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0392326.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            9858..10127
                     /locus_tag="WGO87_000949"
     CDS             9858..10127
                     /locus_tag="WGO87_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0392327.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            10127..11902
                     /gene="ptsP"
                     /locus_tag="WGO87_000950"
     CDS             10127..11902
                     /gene="ptsP"
                     /locus_tag="WGO87_000950"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0392328.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIAKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
BASE COUNT         2977 a         3029 c         3395 g         2742 t
ORIGIN      
        1 tacatgaaaa aatagaaaaa ctgccgggta tggttgaata aggggtcaga ccggttccaa
       61 ttcgctcagt ccgggcaaat ctgcaaaacc gcgttcgcgt atgatttggc aaaaattgtt
      121 cagatagctc ttgtccgtat cttcggtacg gatggcggca tacagtttgc tttgcaatcc
      181 gtcggcagta atttggcggt ggacgacata gcctttttca aggtagggca tgactgtcca
      241 atagggaagg gcggcaatgc cacgtctgct ggcaaccagt tggataatgg cgatggtcag
      301 ttcgctgtgt cggcgcggcg ggttgatgtt tttcggaatc aggatttttt tgggcaaatc
      361 cagcatctcg tcgggaacgg gataagtaat cagggtttcc ccgataaagt cttccgccgt
      421 ccaaacgttt ttggcggcaa gcggatggtc tggtgcgcaa atgccgacca tttcgtaggc
      481 aaacagcggt tggaaagaaa tatcgttttg tttttccgct tcggaaacaa tggcaaggtc
      541 ggcacggtgt tgcagcagca gtccgacggg atccgcttgg aatcccgata cgatatccaa
      601 ttcgacttgg ggccacatcg ggcggaattc gcccatggcg ggcatcagcc agtcgaaaca
      661 ggtatggcat tcgacggcaa tccgcagctc tcccgcctct ccttccgtga ttcgcgccaa
      721 atcccattct gcaacagcaa cttgaggtat aagttcgtgg gcgaggcgca gcagcctttc
      781 gcccaccggg gtaaagcgca agggcgtgga tttgcgttcg aacagcggcg tgccgtagtg
      841 gttttcgagc atacggatct ggtgggaaag agcggattgg gtaaggaaaa cccgtttggc
      901 ggcaagggag acgctgccgg tttcttcaag tgccagcagg gtcttgaggt ggcgcagttc
      961 gataatggaa tccatggggc ttcagacggc atattgaacc ggcgcatatt aaaataattc
     1021 atatgcgggt gcaaatcgga aaaatgtgaa ttccggacat tttgcgatta gaatgcccgc
     1081 ttgtttaaag cgattaggaa aaaagatggt attgcgcagg gattttctga cttggtgtaa
     1141 tgaaacattg cagacagcgt tgtttaaaga ttacgcccct aacggtttgc aggttgaagg
     1201 gagggaatat atcgggaaaa tcgttacgtc ggtaacggca agcagggcag cgattgattt
     1261 tgctgtggag cagaaggcag atttgctttt ggtacatcac ggtatgttct ggaaaaacga
     1321 gttgccgacc gttactggtt ggaaaaaaga acggattgcc gcactgttac ggcacgacat
     1381 caatatggca ggctaccatc tgcccctgga tgcacatccc acactgggca acaatgccca
     1441 actcgccgac agattgggtt ttgcgacaga aaaacggttc ggcgaacaaa acctgctcaa
     1501 ctcgggcagc ctgaaacaag ccaagacact cggcgcattg gcggcgcata ttgaaacagt
     1561 tttgcaacgt aagcctgtcg tcatcggcaa acccgaacgc gaaatccgac gggttgcatg
     1621 gtgcagcggc ggggcgcagg ggttttttca gacggcaata gacgaaggtg tcgatctgta
     1681 tttgacgggg gaaatctctg aagcccaata ccaccttgcc aatgaaacgg gtacggcttt
     1741 catttcggca gggcatcacg cgacggaacg ttacggcgta cgcgcgctgg cagaatcggc
     1801 ggcagaggtt ttcgggttgg aagtgtgcca ttttgacgaa aacaatccgg cttgagtctt
     1861 gagaaatatc ataaaacttt accttatttt aaataatgct ttgagtatca ctgcaaactg
     1921 ggtaaaattg caacgtttaa tggctcggta ttcccaaaat attaggacga ctgaataatg
     1981 gataatcaag aaatcaacaa cggccgccgc cgtttcctga cactcgcgac ctgcggcgcg
     2041 ggcggagtgg cagcattggg tgtggcaacg ccgtttgtgg ccagtttttt cccttcggaa
     2101 aaagccaagg ccgccggtgc tgccgtcgag gtggatgtca gtaaaatcga agcgggtcag
     2161 ctgctgaccg ccgagtggca aggcaaaccg atttgggtgc tcaaccgtac agatcagcag
     2221 cttaaagacc tgaaaggcct gaacggcgaa cttaccgatc ccaattccga tgcggaacag
     2281 cagccggagt atgctaaaaa cgagacccgt tcgattaagc cgaacatcct tgtcgccatc
     2341 ggtatctgca cccatttggg ctgctcgccc accttccgtc ccgacattgc ccccgccgat
     2401 ttgggtgcag actggaaagg cggcttcttc tgcccctgcc acggttcgaa attcgacttg
     2461 gccggccgcg tatataaagg tgttcctgcc ccgaccaacc tggttgtccc gccatataaa
     2521 tacttgagcg acacaactat cttggtgggc gaagactaag aataaggaac gataattatg
     2581 gcaaaccaaa ccaatagcaa agcaaaagca ttgttaggct gggtagatgc ccgttttcca
     2641 ttaagtaaaa tgtggaaaga gcatctgtct gaatactatg cgcctaaaaa cttcaacttc
     2701 tggtatttct tcggctcatt gtctatgctg gtgctggtga ttcaaatcgt cagcggtatt
     2761 ttcctgacca tgaactacaa accggacggc aaccttaacg cctaccatct gcctgctgcc
     2821 tttaccgcag tagagtacat catgcgcgac gtgtccggcg gctggattat ccgctatatg
     2881 cactctaccg gcgcatcttt cttcttcatc gtcgtttatc tgcacatgtt ccgtggtctg
     2941 atttacggtt cgtacaaaaa accgcgcgaa ttggtgtgga ttttcggttc cctgattttc
     3001 ttggcattga tggcagaagc ctttatgggt tacctgttgc cttggggtca aatgtccttc
     3061 tggggtgcgc aggtaattat taacctgttc tccgccatcc ctgttatcgg tcctgatttg
     3121 tccacttgga tccgcggtga cttcaacgtt tccgatgtta ctttgaaccg attcttcgcc
     3181 ctgcacgtta tcgcggtacc tttggtattg ctcggcttgg ttgtggctca tatcattgcc
     3241 ttgcatgaag tgggttccaa caaccctgac ggtgtagaaa tcaaaaagct gaaagatgaa
     3301 aacggtgtcc ctctagatgg catacctttt tttccgtatt atgttgtgca tgatatattg
     3361 gcagtaacga tattcttgat tgtcttctgt gccgtgatgt tctttgcacc tgaaggcggc
     3421 ggctacttct tggaagcgcc aaacttcgat gcagcgaatg cgctgaaaac acctccgcac
     3481 attgcgccgg tatggtactt cactccgttc tacgcaattc tgcgtgcgat tccttccttt
     3541 gccggtactc aggtatgggg tgtaatcggt atgggtgcag cagttgtact gatcgccttg
     3601 ctgccttggt tggataaagg cgaggttaaa tctgtccgct atcgcggccc aatcttcaaa
     3661 accgcattgg ttctgttcat catttccttt atcggtttgg gtattttggg cgcgatggtg
     3721 gcaaccgata cgcgtacttt ggtcgcgcgc atcctgtcct tcgtctattt cgcattcttc
     3781 ctgggtatgc cgttctatac caagctggat aaaaacaaac cggttcccga acgcgtaacc
     3841 atgagcaccg ccaaacaaaa aatcatgttc tttgtttacg tcggtattac cgttgttggt
     3901 gcttacttgt ttgcaaccaa tatctgatga gggcagcgaa aatgaaacaa actctgaaaa
     3961 actggtttgc tgccttattg ctggcagtgc ctatgagtgc agccgtcgcc agcggcggcg
     4021 gacactacga aaaagtcgat atcgacctgc gtgaccaagt cagcctgcag cacggtgcgc
     4081 aaatctttac aaactactgt ttgtcttgcc actctgcaag cggtatgcgc ttcaaccgtc
     4141 tgaaagacat cggtttgact gacgaagaaa tcaagaaaaa cctgatgttt accaccgata
     4201 atgtcggcga tgtcatgcat tcggcgatga acccgaaaga tgcggcaaaa tggtttggtg
     4261 ctgctccgcc cgatttgacg ttgattgcgc gttccaaagg tgcagactac ctttacgcct
     4321 atatgcgcgg cttctataaa gatccgaccc gtccgagcgg ctggaacaat actgtattcg
     4381 ataaagtcgg tatgccccac ccgttgtggg agcaacaagg tgttcaagcc gttgagttgg
     4441 atgccaaagg tcagccggtt atggtaaaag acgaacacgg cgagatgaag cctaagctgt
     4501 attgggaatc taccggtttg cacagccgcc gcctgcctaa cggcaaagtg atccaaaaag
     4561 agtacgacgc atatgtacgc gatttggtca attaccttgt gtacatgggc gaacctgcac
     4621 aactgcaacg caaacgtata ggctatgtcg tgatgatttt cctatttgcg gttatgctgc
     4681 ctttggctta cttcctaaat aaagaatttt ctaaagatat tcattaatca ttaaaaaaga
     4741 atattggaaa gacgtacact aagcgtttgg aacaaaaggg caaatccttt agggtttgcc
     4801 cttttttcat tttgcctgcc gtttgaaaag cctgaatccg tatgccgtct gaacatagct
     4861 gcaacatttc agacggcatc attctaaaaa tgtcagacat cgggaactta atcagggttt
     4921 gtggcagatt gatattgaat taggaaaagg tctgaaaccg caagcagcat ggtaaggcga
     4981 tgtcgaacag gtttaaagcc aatccgctat attttttgga gctatgtcga acaaacaggt
     5041 agaatcggaa taagcggcgg ttttgtccgg tgtgaaaatg tttgaatatt aggatgattg
     5101 atggaactga tgactgtttt gctgcctttg gcggcgttgg tgtcgggcgt gttgtttaca
     5161 tggttgctga tgaagggcag gtttcagggc gagtttgccg atttgaacgc gcaactggcg
     5221 gaaaaggcgg caagatgtga ttttgtcgaa caggcacacg cagaaattgc atcggaattg
     5281 gccgttttgg acgggaaata ccgacatttg caggacgaaa attatgcttt gggcaaccgt
     5341 ttttccgcag ccgaaaagca gattgcccat ttgcaggaaa aagaggcgga atcggttcgg
     5401 ctgaagcagt cgtatatcga gttgcaggaa aaggcacagg gtttggcggt tgaaaacgaa
     5461 cgtttggcaa cgcagctcgg acaggaacgg aaggcgtttg ccgaccaata tgccttggaa
     5521 cgccaaatcc gccaaagaat cgaaaccgat ttggaagaaa gccgccaaac tgtccgcgac
     5581 gtgcaaaacg accttgccga tgtcggcaac cgttttgccg cagccgaaaa acagattgcc
     5641 catttgcagg aaaaagaggc ggaagcggag cggttgaggc agtcgcatac cgagttgcag
     5701 gaaaaggcac agggtttggc ggttgaaaac gaacgtttgg caacgcaaat cgaacaggaa
     5761 cgccttgctt ctgaagagaa gctgtccttg ctgggcgagg cgcgcaaaag tttgagcgat
     5821 cagtttcaaa atcttgccaa cacgattttg gaagaaaaaa gccgccgttt taccgagcag
     5881 aaccgggagc agctccatca ggttttgaac ccgctaaacg aacgcatcca aggtttcggc
     5941 gagttggtca agcaaaccta tgataaagaa tcgcgcgagc ggctgacgtt ggaaaacgaa
     6001 ttgaaacggc ttcaaggatt gaatgcgcag ctgcacagcg aggcaaaggc cctgaccaac
     6061 gcgctgaccg gtacgcagaa taaggttcag ggcaattggg gcgagatgat tctggaaacg
     6121 gttttggaaa attccggcct tcagaaaggg cgggaatatg tggttcaggc agcatccgtc
     6181 cgaaaagagg aagacggcgg cacgcgccgc ctccagcccg acgttttggt caacctgccc
     6241 gacaacaagc agattgtgat tgactccaag gtctcgctga cagcttatgt gcgctacacg
     6301 caggcggcgg atgcggatac ggcggcacgc gagttggcgg cgcatgtcgc cagcatacgc
     6361 gcgcatatga aaggcttgtc gctgaaggat tacaccgatt tggaaggtgt gaacacattg
     6421 gatttcgtct ttatgtttat ccctgtcgaa ccggcctacc tgttggcgtt gcagaatgac
     6481 gctggcttgt tccaagagtg tttcgacaaa cggattatgc tggtcggccc cagtacgctg
     6541 ctggcgactt tgcggacggt ggcgaatatt tggcgcaacg agcagcaaaa ccaaaacgcg
     6601 ctggcgattg cggacgaagg cggcaagctg tacgacaaat ttgtcggctt tgtacagacg
     6661 ctcgaaagcg tcggcaaagg catcgatcag gcgcaaaaca gctttcagac ggcatttaag
     6721 caacttgccg aggggcgcgg gaatctggtc ggacgcgccg agaaactgcg tctgttgggc
     6781 gtgaaggcaa acaagcagct tgcacgcgat ttgaccgaac gtgccaatga aacaacggcg
     6841 ttgtcggaat ctttggaata tgtggcagaa gatgaagcag tctgacttgt gcggaaaagc
     6901 ttgtttcagc aggggcggga atgccgaaag cgcggcgtaa ctgtaccggt tctgatttgg
     6961 ggtttgtttg aagtacttac caaagccttg tccggcgagg tacaccggca aggcggcggt
     7021 tgtctgagcc ttttgcgttt cagacggcat cagtgcagat gacgccgtct gaagaccgta
     7081 gtgaagtcgg ttagaaaaac ggattgtgcc gtttttcgtg tccgatggaa gtcatacgcc
     7141 cgtgtccggc gacaacttgc acggtttcgg ggagggcgaa taatttgttg cggatattat
     7201 tgattaagtc ggcgtggttg ccgcgcggaa aatcggttct gcctatggtt tcgtaaaaca
     7261 gcacgtcgcc cgcaatcagc aattccgcct cggcacaata aaagacgata tgtcccggcg
     7321 tatggcccgg aatatgcagc acttgaaagg catagcgtcc gaccgtgagc gtttcgcctt
     7381 cttcgagcca acggttcggc gcaaaggcgg gcgagacggg aaatccgtat tgcgcggtgg
     7441 tttgcggcag cgattggagc aggaattcat cgtccggatg cggcccgagg acgggaactt
     7501 tatgcgtttt caacatttcg accacgccgc ccgcgtgatc gagatggccg tgcgtcagcc
     7561 agattgccgt gagcgtaagt ttgcggtttg ccaacgcttg cagcaggaac ggcacatcgc
     7621 cgccgacatc ggtcaggacg gcttcgccac tttcgtcgtc ccaaatcagg gtgcagtttt
     7681 ggcggaaggg ggtaacgggg aagatttcgt aacgtagggt catcggcgtg ttcctaaacg
     7741 gtttttcaga cggcatcggg tttgccgttt gttttatggc ggtttgccgc ctgtttggat
     7801 attgggggaa tcgggtgatt aagaagacaa tcggcggcat cataccgatt tttatggcgg
     7861 ttttcatccc tgcatcggca ggcgcggcgg atttgatgct ggcgcaggaa tacaaagggc
     7921 aggatattgc cggctgggcg atgagcgaga aactcgacgg cgtgcgcgcg tattgggacg
     7981 gaaagcacct gataagccgt cagggctatg cgtttacgcc ccccaaaggt tttaccgctc
     8041 agtttccgcc ttatcctttg gacggcgaat tgtatagcgg acgcggtcag ttcgagcaga
     8101 tttccgccgc cgtgcgttct gcttcttcag attggcgcgg catccgcctg cacgttttcg
     8161 atgtacccaa ggcgcagggc aatctctacc aacgtttggc agtcgcaacg cagtggctga
     8221 aaacgcatcc gaacgcgccg attaccatca tcccgcaaat caaagtgcgc gaccggcagc
     8281 acgcgatgga ctttttaaaa caaatcgaag cgcagggcgg cgaaggcgtg atgctgcgtc
     8341 agcccgaatc ccgttacagc ggcggcagga gcagccaatt attgaagctg aaaagccaat
     8401 acgacgacga atgcacggta acgcggcact atgagggcaa agggcgaaac gccggacggt
     8461 tgggcgcggt cggctgcaaa aaccgacacg gcgaattccg catcggcagc ggcttcaaag
     8521 acaaagaccg cgacaacccg cccaaaatcg gtacgctgat tacctaccgt taccgtggct
     8581 ttacgcagaa aggcacgccg aaattcgcca cgtttgtgcg cgcgcgtacc gaccgttgag
     8641 caggacggtt cagacggcag ccgatacttt ggtttataat tttcctttta ccgaccgatt
     8701 ccgacatatg accgatttag aaaccaaacg ccttgaaaca caggcgatgc ttgaaaacgc
     8761 cgatcttttg ttcgaccaag gccaatgccg tgccgcactg caaaaagtgg cggacgagat
     8821 tacgcgcgat ttgggcggca aatatccgct gctgctgccc gtgatgggcg gcgcggtggt
     8881 gtttacgggg cagttgctgc cgctgttgcg ttttccctta gattttgatt atgttcacgt
     8941 ttcccgttac ggcgacaagc tggagggcgg cgcgttcaac tggaagcgta tgcccgatgc
     9001 ggaacaaatc cggggcaggc acgtcgtcgt gctggacgat attttggacg aagggcatac
     9061 gatgtccgcc attcaagcca aacttttgga aatgggtgcg gcaagctgcc gtgcggcggt
     9121 gttcgccaac aagctgatcg acaaacccaa gcctatccga gccgattatg tcggactgga
     9181 tgtgccgaac cgttatgttt tcggttacgg catggatgcg gcgggctgct ggcgtaattt
     9241 gggcgagatt tacgcattgg acggaaaata aggacgcgat gccgtctgaa ggcctttcag
     9301 acggcatcgc gcccatacgc cggcaggata atgaagaaca ggacgcataa tatgataggg
     9361 cttttaatca tcacacacga aaccataggc gaagcctacc gcaagctggc acatcatttt
     9421 ttcccgggcg ggctgcctga aaacgtccgc atactcggcg tgcagccgac ggaagaccaa
     9481 gacgacatca tcaacaacgc cattgccgcg cttcaggagt ttcccgacaa cgacggcgtg
     9541 ctgattatga ccgatatttt cggcgcgacc ccctgcaatg cagcccgccg cctcgtgcgc
     9601 gaaaacaaat cggcgatttt gaccgggctg aacgcgccga tgatggttaa ggccgtccaa
     9661 tattcgccgg cggcggaaga ccttgccgcc tttaccgaat gtgtcagaga ggcggctgta
     9721 aaaggcattt tcgccatcac gtccgcgccc gaagatttgg tgtgccggcg cagcggcgat
     9781 gccgtctgaa gaagcggcag ggcaggaaaa cattttaaac ggcgtctgct gccgatatac
     9841 ataacacggg aaccgaaatg ctcaaacaat ccatcgaaat catcaacaaa ctcggactcc
     9901 acgcccgcgc gtccaacaag ttcacccaaa ccgcgtccca attcaaaagc gaagtctggg
     9961 ttacgaaaaa cgacagccgc gtcaacggca aaagcattat ggggctgatg atgctcgccg
    10021 ccgccaaggg tacggtcatc gagctggaaa ccgacggcgc ggacgaggcg gaagcgatgc
    10081 gcgccctgac cgacttaatc aacggctact tcggcgaggg cgaataatga gtatcgtgct
    10141 gcacggcgtg gcggcgggca aaggcattgc cgtcggttgc gcccacctga ttgcgcgcgg
    10201 tacggaggaa gtgccgcagt atgatgttgc ggaggcggac accgatgccg aagccgaacg
    10261 tttcgatgcc gccgtcaaag ccacgcgcaa agagttggaa cagctccgca gcgcgattcc
    10321 cgaaaacgcc ccgaccgagt tgggcgcgtt catctcgctg cacctgatgc tcttgaccga
    10381 tgttaccttg tcgcgcgaac ccgtcgatat tttaagggaa caaaaaatca acgccgagtg
    10441 ggcattgaag cagcagagcg acaaactcgc cgcccaattc gacaatatgg acgatgccta
    10501 tttgcgcgaa cgcaagcagg atatgctgca agtcgtccgc cgcatccaca acaacctgat
    10561 cgggcagggc aacgagttgg aagttgccga caacctgttt gacgaaaccg ttctgattgc
    10621 aaacgacctt tcgcccgccg acacggtttt gtttaaagag cagcgcattg ccgccttcgt
    10681 taccgatgcc ggcggcccca ccgggcatac ggcgattttg ggcaggagct tggacatccc
    10741 gtccgtcgtc gggctgcaca acgcgcgcaa actgattacc gagggcgaaa cggtcattgt
    10801 ggacggtatc aacggcgtgt tgattatcgc gccggatgag tcggtgttga acgaataccg
    10861 ccgccgtgcc cgcgaatacc gcagccacaa acgcgatttg aacaagctca aaaaaaccgc
    10921 cgccgccacc gctgacgggg tctgcatcga gcttgtgggc aatatagaat ccgccgaaga
    10981 cgtgaaaccg ctgcacaacc tcggcgcaga cggcatcggg ctgttccgca gcgagtttct
    11041 ttacctgaac cgcgatacga tgccgtctga agacgagcag tacgaagtgt acagcgcgat
    11101 tgtcaaaaaa atgaaaggca aaagcgtaac gatacggaca gtcgatttgg gtgtggacaa
    11161 aaacccgcgc tggttcggga aaaacagcac gcccaacggc agcctcaacc ccgcgctggg
    11221 catgaccggc atccgcctgt gccttgccga accggtcatg ttccgcaccc agatgcgcgc
    11281 catcctccgt gcggcggtac acggccccgt gcggatgatg tggccgatga ttacctccgt
    11341 atccgaagtg cgccagtgcc tcatccacct cgacaccgcg caacgccagc ttgccgaacg
    11401 cggcgatgcc ttcggtaaag tcggcatcgg ctgtatgatt gaaattccgt ctgccgcgct
    11461 gaccgtcggc agtattttga aactggtcga tttcatctcc gtcggtacca acgacctgat
    11521 tcaatacatc ttgtccgtcg atcgcggcga cgacagcgtc agccacctct accagcccgg
    11581 ccatcccgcc gtgctgaaaa tgctgcaaca cgtcatccgt accgccaacc gcatggacaa
    11641 agacgtatcc gtatgcggcg agatggcggg cgataccgcg tttacccgcg ttttattggg
    11701 tatggggctg cgccgttttt ccatgaaccc caacaacatc ctgcccgtca aaaacatcat
    11761 tctgcacagc aatgtcggac agctcgaaag cgatattgcg aaagtcatcc gctgcgaaga
    11821 cgaagagaag tcggaaaagc tgatcaaaca gatgaacagc gtgtctgtcg aggaagaagc
    11881 cgacttcaag gggcggaaat aaatacggca ggtaaaaaat agaaatactt aacaatgccc
    11941 gcaatctgaa attttgccat tcttgcaaaa tagaaaaccg aaacagaaac ccaaaatcgg
    12001 tcattccctc aaaaacagaa aaccaaaatc agaaacctaa aatccgtcat tcccgcgcag
    12061 gcgggaatct aggtttgtcg gcacagaaac ttgtcgggaa aaacggtttc tttagatttt
    12121 acgttctaga ttcccgcttt tgc
//
LOCUS       DAWFBO010000048        12124 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFBO010000048 DAWFBO010000000
VERSION     DAWFBO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.048"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            15..286
                     /locus_tag="WGO87_000951"
                     /pseudo
     CDS             15..286
                     /locus_tag="WGO87_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            326..1360
                     /locus_tag="WGO87_000952"
     CDS             326..1360
                     /locus_tag="WGO87_000952"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0392329.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1566..2147
                     /locus_tag="WGO87_000953"
     CDS             1566..2147
                     /locus_tag="WGO87_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0392330.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2216..2398
                     /locus_tag="WGO87_000954"
     CDS             2216..2398
                     /locus_tag="WGO87_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0392331.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            2395..3156
                     /gene="kdsB"
                     /locus_tag="WGO87_000955"
     CDS             2395..3156
                     /gene="kdsB"
                     /locus_tag="WGO87_000955"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0392332.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            3179..3496
                     /locus_tag="WGO87_000956"
                     /pseudo
     CDS             3179..3496
                     /locus_tag="WGO87_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3601..3798
                     /locus_tag="WGO87_000957"
     CDS             3601..3798
                     /locus_tag="WGO87_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392333.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            3955..4047
                     /locus_tag="WGO87_000958"
     tRNA            3955..4047
                     /locus_tag="WGO87_000958"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3989..3991,aa:Ser,seq:gct)
     gene            4350..5135
                     /locus_tag="WGO87_000959"
     CDS             4350..5135
                     /locus_tag="WGO87_000959"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0392334.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGMLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            5172..6044
                     /locus_tag="WGO87_000960"
     CDS             5172..6044
                     /locus_tag="WGO87_000960"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0392335.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRREMKQRISG
                     LITLLCRQDKVSAA"
     gene            complement(6104..6715)
                     /locus_tag="WGO87_000961"
     CDS             complement(6104..6715)
                     /locus_tag="WGO87_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0392336.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHDK"
     gene            6824..7048
                     /locus_tag="WGO87_000962"
     CDS             6824..7048
                     /locus_tag="WGO87_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0392337.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            7049..7195
                     /locus_tag="WGO87_000963"
     CDS             7049..7195
                     /locus_tag="WGO87_000963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392338.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            7411..8445
                     /gene="pyrC"
                     /locus_tag="WGO87_000964"
     CDS             7411..8445
                     /gene="pyrC"
                     /locus_tag="WGO87_000964"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0392339.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAVLPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQDILFLVHGEVTDPEIDIFDREAAFIERVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(8812..9237)
                     /gene="nusB"
                     /locus_tag="WGO87_000965"
     CDS             complement(8812..9237)
                     /gene="nusB"
                     /locus_tag="WGO87_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0392340.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAIPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(9315..9791)
                     /locus_tag="WGO87_000966"
     CDS             complement(9315..9791)
                     /locus_tag="WGO87_000966"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0392341.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRILQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(9839..10162)
                     /locus_tag="WGO87_000967"
     CDS             complement(9839..10162)
                     /locus_tag="WGO87_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0392342.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            10329..11048
                     /gene="rnc"
                     /locus_tag="WGO87_000968"
     CDS             10329..11048
                     /gene="rnc"
                     /locus_tag="WGO87_000968"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0392343.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            11095..12018
                     /gene="era"
                     /locus_tag="WGO87_000969"
     CDS             11095..12018
                     /gene="era"
                     /locus_tag="WGO87_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0392344.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         2793 a         2911 c         3543 g         2877 t
ORIGIN      
        1 aaagatactg gaaaatgaaa gagatgaaac ctgtccgtta tcatattggc gatatgcccg
       61 agacttcaaa acaaaccgcc tcccgtcatg acgacagggc agtgggtgtt gacgatgatt
      121 gttttcatga ttcctttggt caatttttgt ttgggtgttc ggcagaggca acccgaaccg
      181 cgccaatttc tgtaaagcgc agttgcttat ttacctgatt ggttcgctta tcggtttggt
      241 cttcgcgttg tttataggtg ggtctgtatc gggtacgcat gattaatgcc ccgggctgat
      301 tttgcttcga ggatttgtat cgaatatgcc gaattgtttc aaatttcata ccgttatcga
      361 acggcattgg caaaaacctt atccggtttt gtcttttctg cttaagccgc tctccgggct
      421 gtttgccaaa attgcggcaa aacggcggac ggatttttta tcgggaaaac ggcaaagcga
      481 aaagctgccc gtgcctgtgg tcgtggtcgg caatattcac gcgggtggga cggggaaaac
      541 gccgattgtt gccgcgctgg tgtcgggttt gcaggaaaag ggcgtcaagg tcggcatcat
      601 cagccggggc tacgggcgca agagcaaggc ggttcatgta ttgaatgctg agagccgagc
      661 ggaagatgcg ggcgatgagc ctttgctgct gttccgcaaa accggtgcgc cgacggcggt
      721 gggcagcagc cgtgcagagg caggcagggc gttgctggcg gcgcatcccg acatcggact
      781 gattgtggcg gacgacggtt tgcagcatta cgccctgcgg cgagatgtgg aaatcgcggt
      841 gtttccggcg gcggatacgg ggcgcacgga tttggattta ctgcccaacg gcagtttgcg
      901 cgaacctttg ttgcggctgg attcggtgga tgcggtcgtc gtcagcggcg gcaaggcgga
      961 tgcgctgttt aggccgtctg aaaatatgtt tcacagccgt atcgaagcgg gacggattta
     1021 ccgtttgaac aatccgtccg aaatactgga cacaggccgt ctgaaaaatc aaaccgtcgt
     1081 cgccgtggca ggtattgcca agccggcgcg ttttttcgat tcgttgcgga atatgggcat
     1141 taccgtgaag cgaaccgtcg cgctgcccga ccacgccgac atttcggcgg cagatttgcc
     1201 cgatgcggac gcggtcatta ttacggagaa agatgcggtc aaattttcag acggcatttg
     1261 caccgataat gtttgggtgt tgcccgtttg tgcgataatc gaacctgatt tggcggcgtt
     1321 tgtgttggag cggttggaag atgtaccgaa ggccgtctga aagcacggtt tgggcggagt
     1381 gattacggat ttgaataaga acgcctcgcg ccgtcattcc cgcgcaggcg ggaatctaag
     1441 tctcgaattt tcaggaatgc ctaggaggct tcagaaatcc caaatctccg gatttccact
     1501 tggacaggaa tgagaaaatc ggtcgtattt tttatctgca ttaatcattc gttaaagtat
     1561 tgaatattaa actgaaaacc ttgttattgc ccttcgccac gctggcattg tgcaccaacg
     1621 cttttgccgc cccgcccagc gacgcgtcgt tggcgcgttg gctggatacg cagaattttg
     1681 accgggatat agaaaaaaat atgattgagg gctttaatgc cggatttaaa ccgtatgcgg
     1741 acaaagccct tgccgaaatg ccggaagcga aaaaagatca ggcggcagaa gcctttaacc
     1801 gttatcgtga gaatgttttg aaagatttga ttacgcccga agtgaaacag gctgtccgta
     1861 atactttatt gaagaatgcc cgtgagatat acacgcaaga agaaattgac ggcatgattg
     1921 ccttttacgg ttcgcctgtc ggtcagtccg tcgttgccaa aaatccgcgc ttaatcaaga
     1981 aatcgatgag tgaaatagca gtatcttgga ctgcattgtc agggaaaatc gcgcgacatc
     2041 atctgcccgc gtttacggaa gagttgcggc gcatcatctg cggcggtaaa aatcccgatg
     2101 cgggctgtaa acaagccgga caggttggga aaaggcatca gaaataaatg atagccgtct
     2161 gaaatattga agagggcatc cgattgattg aaccatcaaa cccgaaagca accctatgga
     2221 aaaaaaattc ttagacatcc tcgtctgccc cgttaccaaa ggcaggctgg aatatcatca
     2281 ggacaaacag gaattgtgga gccgtcaggc gaagcttgcc tatccgatta aagacggcat
     2341 tccctatatg ctggaaaacg aagcgcgtcc gttgggcgaa gaggaactcg aagcatgacc
     2401 gaattcgtcg tattgattcc ggcgcggctg gattcgtcgc gcctgcccgg aaaagccttg
     2461 gcggacatcc acggcaaacc gatggtcgtg cgcgttgccg aacaggcggc aaaaagtaaa
     2521 gctgtgcgcg tcgtcgttgc caccgaccat cccgatattc agacggcctg tcaggcgcac
     2581 ggcgtcgaag tcgtcatgac ttcaaaccgg cacgaaagcg gcacgacgcg ccttgccgaa
     2641 gcctctgccg cgctgaagct gccgccgcat ctggtggttg tcaacgtaca gggcgacgag
     2701 ccgctgattg cacctgaact catcgaccgc accgccgaag tactcgtcga aaacaacgtc
     2761 caaatggcga ccgccgccca cgaattgcac gatttcgacg aattgatgaa tcccaatgcc
     2821 gtcaaagttg tcctcgacaa aaaccgcaac gccatctact tcagccgcgc cccgattccc
     2881 tatccgcgcg atgcgatgcg tgcaggaaaa cgcgaaatgc cgtttgaaac cgccgtcctg
     2941 cgccatatcg gcatctacgc ctaccgcgtc ggcttcctgc aacgctacac cgaaatgagc
     3001 gtttcgccgc tggaaaccat tgaatcgctg gagcaactgc gcgtcctgtg gcacggctac
     3061 ccgattgccg tcgaaaccgc caaagaagct cctgccgccg gcgtagatac gcaggaggat
     3121 ttggacagga ttcgcgctgt atttcagact atataaaata gattcaaagg gaaaagatat
     3181 gcagcaacat attgaaaaat ggcaacactt gagccgggaa gaacagaaaa tccttgctga
     3241 agtatggggt ctcgtgcaaa acgacgatca ggaggttcac tatgaaatgc tcaaattgaa
     3301 cgcacccgac gaagccagtg gcgaattttg gttcagaatg gcagaaacac tcagcaccct
     3361 gccgcccaac cgttccctcg accttagaat gaacggcggc aggctggcga ccgccgtatc
     3421 catcctttcc gtcatgattg aagacaatcc cgacataccg cagctttggg cgcaaaaaat
     3481 taccgcgctc aattatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgtcg
     3541 tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
     3601 ttggcacacg gacacaaagc ccgtgccgac ggtttggcac aacagcccga caaagcggca
     3661 gaagccaacg aggaggaata cctgaccaaa gccctgtcgc aaaacctact gtcaacattg
     3721 gatgccgcgc ttgcacgttt tcctgaagac gcgtggtttc aggaaatcaa acaggatgca
     3781 caaaagcatt ttgcttgagg atgtggcagt caggaatatt tccattcagg aagaaaagaa
     3841 gtgcctgatt gggtataatc agggtaaatc ttattttatt tcaaaagatt aatatttgct
     3901 ttctgttttt ccttgacggt atcggaaaag ttgattatag ttacagcttc cttaggagta
     3961 atggctgaga ggctgaaggc acttccctgc taaggaagca tgtggggtca acctgcatcg
     4021 agggttcgaa tccctcttac tccgccaaga tgagaagacg ctgtgtttta cagcgtcttt
     4081 ttttacgcgc tttgttgcgg aagtgccgag ggctgtctgc cgtgcgccgc ctggatttgc
     4141 aatgtgcccg cccgtgcaaa atccgatttg accaacctga agggcaggtt tcggaagagc
     4201 ggggaatttt gataaacaaa aaactgcacg gcataatctt tcccggtttt cattttccgt
     4261 attcgcctat ccgtaccgca aatgctatac tgggaaaaat ttaccgattg tgttttacgg
     4321 catatttgcc gataggatgg aagagacaaa tgagcagaat ccggcaggct tttgccgctt
     4381 taaacggtga aaaggcattg attccttata ttacggttgg agacccggat attggggcga
     4441 ctttggcaat aatgcacagt atggttgcaa acggtgcgga tattttagag ttgggcgtgc
     4501 cgttttccga tccgatggcg gacgggccgg tgattcagcg tgcggcggag cgggcgttgg
     4561 caaacgggat ttcgctgcgc gatgtcttgg atgtcgtcag aaaattccgt gaaaccgaca
     4621 cgcaaacgcc ggttgttttg atgggatatt tgaaccctgt acataagatg ggttatcggg
     4681 agtttgctca ggaagccgca aaggcgggtg tggacggcat gttgacggtg gattcccctg
     4741 tcgaaaccat cgatccgctc tatcgcgagc tgaaggataa cggggtcgac tgtattttcc
     4801 tgattgcgcc gacgacgacg gaagaccgta ttaaaaccat tgccgagctg gcaggcggat
     4861 ttgtctatta tgtttcgctc aagggcgtaa cgggcgcggc aagtttggat acggatgagg
     4921 tttcgcgtaa aatagagtat ttgcatcagt atatcgatat tcccatcggt gtcggtttcg
     4981 gcatcagcaa tgcggaaagc gcgcgcaaaa tcggccgggt tgccgacgcg gtcattgtcg
     5041 gcagccggat tgtgaaagag attgaaaaca atgcgggcaa cgaggctgcg gccgtcggtg
     5101 ctttggtcaa agagttgaag gatgccgtgc gctgattctg tcacgcattc taaatatttt
     5161 aggagttgtc catgagctgg ttagataaaa tcctgccacc caaaatcaaa aaccgcagca
     5221 aagacggttc ttccaatgtt cccgagggtt tgtggcacaa atgtccgtct tgttcggcaa
     5281 ccgtttattc gaccgagttg cagcagaaca atcaggtttg tccgaaatgc aaccaccaca
     5341 atccgttgtc ggcacgacaa cgcctgaatc tgcttttgga tgaggatggc agagaggagg
     5401 ttgccggaaa tgtcaaaccg acagatcctt tgaagtttaa agacggcaaa aaatatccgg
     5461 atcgtttgag tgcggcacgc aagctgaccg gggaagatga tgctttggtg gtaatgaaag
     5521 gcaagatgaa cggtctgccc gttgtggttg ctgcgtttga gttccgcttt atcggcggtt
     5581 cgatgggttc ggttgtgggc gaacgattcg tacaaggtat ccgtcgggcg gttgccgaca
     5641 attgtccgtt cgtctgtgtg gcggcttccg gcggtgcgcg tatgcaggag ggtgtgaact
     5701 cgttgatgca gatgacgaaa accagtgccg cgctgcattt gctgacggaa aaacgcctgc
     5761 cgtttatatc ggtattgacc gatccgacta tgggcggcgt atccgccagc ttcgcatttt
     5821 tgggcgatgt cgtgcttgcc gaacccaatg cattaatcgg ctttgccggt ccgcgcgtga
     5881 ttgagcagac ggtgcgcgaa acgctgccgg aaggcttcca gcgtgccgag tttttgctgg
     5941 aaaaaggcgc aatcgaccag attgtcgacc ggcgcgagat gaagcagaga atcagcggtt
     6001 tgattacgct gttgtgccgt caggacaaag tttctgccgc ctgatggctg atgaatcgaa
     6061 taccgtctga aaccgatgtt tcagacggta tttttgtgtc tggttatttg tcgtgcggct
     6121 tcatcgacgg ggcatagcgt ccggcacgtt ctttcaggcg ttgtaccaaa cctttcgtgt
     6181 cggcgggtac accgccctcg cagaatgcct gatacaggac ggtgcgcagt gcgtcgttgc
     6241 ggcttaatgt accgcctatc ggtttccatt cggcgtttcg gggctgtatc cagcggcggt
     6301 tgaccgtgtc gccgtagccg aacactttat aggaggaagg tttgccgttg ccgaacctga
     6361 tggagaagcg ggcgcagaat atgccttcgg cagtcaggtt gtcgtagcct ttgtcggagc
     6421 ggatattgag aatgtagcgg atgctgccgt cgggcgcggg cataatttgc aggctgtcga
     6481 gcaggatttt cggctgtttg ccgtaatttt catccacata aatgtcaaac cagccgtccg
     6541 agtgcgtatc gggcagcggc ggcagtttgg cggtatgttc tttaaattcg cgggcggcgg
     6601 cttcttcggg cgtttcgcgg tagcgggtgt tgatcggcgt gtctttttgg ttgaagccgg
     6661 cagcaaggga cgtgccggcg gtgagtgcga gcagcaggag ggcggtgcgg cgcataagtt
     6721 tctccaaatt gaaaacggcg ttattttatg ggttggcaaa gggggctgca agcaactggg
     6781 gtataatctc cccccgattc ccatttttta aacggtacaa acgatgaaca gcgaaacttt
     6841 agacgtaacc ggattgaaat gtcccctgcc gattttgcgg gcgaaaaagg ctttggcgca
     6901 aatgcagcag ggcgacgtgt tgaccgttct ggcaaccgac ggcggcgcac cgggggattt
     6961 tgaggctttt tgccgccaaa ccggtcatgt gctgttggat gcttccgaac aggacggcgt
     7021 gttcacgctg gtcgtcaagc acaaataaat gccgtctgaa atgcggatgt cccgcccgac
     7081 ggcgttgttt ttgaatatcg tatgtgccgc gtgccgtttc aaaaacaaac cgtccggcgt
     7141 gcagtgtcgg catttcgggc gtatcgccgc cgatttgttc cggaatggct gttaaccgcc
     7201 ttgccgtcgg caaagaagca aaacccaagc acaatcaaaa tctaaaggct gtgtttgaag
     7261 attccgttga tgcaacccaa tcagtcggac aaaatgcctt tcatccaatg gaaccggttt
     7321 ccgaccggac ggaataaccg gctttccccc ggcaaacgga tggaatcgac cgggtattca
     7381 aacgcagcca aaacctaaaa aggaacaacc atgcaaaccc tgaccattat ccgccccgac
     7441 gatatgcacc tgcacctgcg cgacggcgac gcgctcaaag ccgttgcccc ttataccgcc
     7501 cgccaaatgg ggcgcgccgt cattatgccc aacctcaaac cgcccgtcgt cagtgtagcc
     7561 gacgcgcttg cctacaaagc gcgcattatg gcggtgttgc ccgaaggcag cgcgtttgag
     7621 ccgctgatga cgctttacct gaccgaccaa gccacgcccg agctggtgcg cgaagccaaa
     7681 gccgccggca tcgtcgcctt caaactctac cccgccggcg cgacgaccaa ttccgattcc
     7741 ggcgtaaccg acctattcaa gctcatcccc gtgttggaag aaatggcaaa acaggacatc
     7801 ctgttcctcg ttcacggcga agtaaccgac cccgaaatcg acattttcga ccgcgaagcc
     7861 gcctttatcg agcgcgtgat gaaacccgtt ttggcgcaag tgccgaatct taaagtcgtg
     7921 tttgaacaca ttaccaccgc cgaagccgcc cgcctggttt tggaagcagg cgacaacgtt
     7981 gccgcctctg tgaccccgca acacctcctg ctcaaccgca acgacctctt ggtcggcggc
     8041 gtgcgccccc atcatttctg cctgcccgtc ctcaagcgcg aaacccaccg tcaggcattg
     8101 gttgccgccg ttaccggcga gaaggcgcac aaattcttcc tcggcacaga ttccgcgccg
     8161 cacgccaaat ccgctaaaga aaacgcctgc ggttgcgccg gtatgttcag cgcgatgacc
     8221 gccatcgagc tttacgccga agtgtttgaa aaagcaggcg cgttggacaa actcgaagcc
     8281 tttgcctcga aaaacggcgc aaggttttac ggcattccgg aaaacgccgg cacgatcacc
     8341 ctcgtcaaac aaagccaaac cgtccccgca agtgttccct acggcgacgg cgaacttgtc
     8401 ccgatgcgcg cgggcggcga aatcggctgg acggtacagt attgattgaa cggtcaagca
     8461 aaatgccgtc tgaaaggttt ttcagacggc atttgtgttg ttgactgatt gatacgtcaa
     8521 cggcgtttga gtttgttacg tttcggttat ttccgataaa ttcccacaat tttcaaattt
     8581 cgccattccc acgaaggcag gaatccggaa attcgatgcg accagagttt atcaaaaacg
     8641 gcagcaactc aaaaaaccgg attcccgcct gcgcgggaat gacgagattg aagtttcaga
     8701 atttatttga aatacccaaa attcaaaaaa ccaaattccc acatgcgtgg gaatgacgaa
     8761 acaaagaaag cagaaataag gacatagaac tttctttaaa tttgtgatgc atcaacggcg
     8821 tttgggctcg tcggggcgga tttgggcagc gagtttgtcg aggatgccgt tgacgaattt
     8881 gtgcccgtcc gtaccgccga aggttttggt aacttcgatg gcttcgttga taatgacggg
     8941 gtagggcgtt tcgggtatgg cggacagctc gtggcaggcg gtcagcagga cggcgcgttc
     9001 gatggggttg aggtcttttt cgtccctgtc gagcagcggg cggatttgcc ggatgtattc
     9061 tgccgcattg gtttgcgtgc cgaagaaaag tttgttgaac aattcttcgt ctgccttggc
     9121 aaagtcggac atttcgcgga tgtttttagc aatctcgggt gctgcggtgc ggttgataag
     9181 ggattggtaa acggcttgta cggcaagctc gcgggaacgg cggcgggctg ttttcatgat
     9241 ttttccttga aacggttggg cggcacggta tgccgtctga aacggaaagg gcgtattggt
     9301 gtacgccctg tttgttattc ttcgtcttca aactgttctt cgagcagcag gttgacgagg
     9361 ttggcgcatt ctacggcgac tttggcggca tccgaggctt tttcttcaat ccgttcgatt
     9421 gcctgcgcgt cgttttcggt ggttaagacg gcattggcaa tcgggatgtt gtagtcgagt
     9481 gcgacgcggc taacccctgc tccggattcg ttggaaacca gctcgaaatg gtaggtttca
     9541 ccacggatga cgacaccgat ggcaatcagt gcgtcaaatt tttcggaaga ggcaagattc
     9601 atcagcgcga tggggatttc aagcgcgccg ggtacggtgg cgacggtaat gttttcgtct
     9661 gccacgccca attcttggag gatgcggcag cagactttga gcatttcgct gccgatttcg
     9721 ttggtgaagc gtgcctgtac gatgccgatg cggaggtgtt tgccgtcgag gttgggggcg
     9781 atggtgttca ttgggtgtcc tttggtattc ggaggtttcg gaatgccgtc tgaaggtttc
     9841 agtcttgcgg ctgccagtcg gcgacggttt ggaatgtgcc gtcttcggca agctcccatg
     9901 cgctgccttc gggttgggaa agcagtgcgg cggtttcagg gttggttttg gcgatgtcgg
     9961 cgaggctgac gatgctgaag ttgtccggat cgtcggtgta ttcgtcggtc tcgtcgccgc
    10021 tgaagaaacg ccagccgctg tcgttttcaa aaacgggggc ttcgcggtag aggaagccga
    10081 cgggccggtt ttgtttggcg acggtgttgg tggcgataca gcggtcgagt gccgaggaaa
    10141 gtgcttgtgc aaatgcgttc attacgggaa tacgttgggg gaaaacttac ggattttacc
    10201 acgattcgtg cgttgtcggc agacggcggc ggtttggtgg tacaatgtgc gccgtttgca
    10261 gccttaaggt gtttctgtat ttttggagta tggaaacgca ttcgggctgt tttttgcgga
    10321 agacggtaat gaaagacgat gttttgaaac agcaggcaca cgcggcgata cagaagaaac
    10381 tgggctacgc gttccgcgat atttcgcttt tgcggcaggc tttgacgcac aggagccatc
    10441 atgcgaagca caacgagcgg tttgagtttg tcggcgattc gattttgaat tatacggtgg
    10501 cgcggatgct gtttgacgcg tttccgaagt tgaccgaggg cgagttgtcg cggttgcggg
    10561 caagtctggt caatgagggc gtgctggcgg aaatggcggc ggaaatgaat gtcggcgacg
    10621 gtctgtactt gggggcgggc gagttgaaga gcggcggctt cagacggcct tcgatactgg
    10681 cggacgcgat ggaggcgatg tttgcggcgg tcagcttcga tgccgatttc aacacggcgg
    10741 aaaaggtggt gcgccatttg tttgccgacc gcgtccggtg tgccgatttc caaaatcagg
    10801 caaaagacgg caaaactgct ttgcaggagg cgttgcaggc acgccgcttt gctttgccga
    10861 aataccgtat cgaagagcaa atcggccatg ccaacgacag tatgtttgtg gtttcctgcg
    10921 atttgggcga actgggtttc gtgtgccgtg ccaaagggac gagccgcaag gcggcggaac
    10981 aggaggcggc gaaagaggct ttgaaatggc tggaagagaa gctgccgctg aagaagaaaa
    11041 agaaatgagg cggcgcgtga atatgccgtc tgaaatgtgg atatgaaagc gaatatggat
    11101 attgaaacct tccttgcagg ggaacgcgcc gccggcggat accgttgcgg cttcgtggcg
    11161 attgtcggtc gtccgaacgt gggcaaatca acgctgatga accatctcat cggtcagaaa
    11221 atcagcatca ccagcaaaaa ggcgcagacg acgcgcaacc gcgtaacggg gatttatacc
    11281 gacgataccg cgcagttcgt gtttgtcgat acgcccggct ttcaaaccga ccaccgcaac
    11341 gcgctcaacg acaggctgaa tcaaaacgtt accgaggcgc ttggcggcgt ggatgtggtg
    11401 gttttcgtcg tggaagcgat gcgctttacc gatgccgacc gtgtcgtgtt gaaacaactg
    11461 cccaagcaca cgccggtcat tttagtggtc aacaaaatcg acaaggacaa ggcgaaagac
    11521 cgttacgcgc tggaggcgtt tgttgcccaa gtgcgcgccg aatttgaatt tgcggcggcg
    11581 gaggcggtca gtgcgaaaca cggattgcgg attgccaacc tgttggagct gctcaagccg
    11641 tatctgcccg aaagcgtgcc gatgtatccc gaagatatgg ttacggacaa atcggcgcgt
    11701 tttttagcga tggaaatcgt gcgtgaaaaa ttgttccgct atttgggcga ggaattgcct
    11761 tatgcgatga acgtcgaagt ggagcagttt gaagaggaag acggtttgaa ccgcatctat
    11821 atcgccgttt tggtcgataa ggaaagccaa aaggcgattt taatcggcaa aggcggggag
    11881 cgtttgaaga aaatttccac cgaagcgcgg ttggatatgg aaaaactgtt tgataccaaa
    11941 gtatttttga aggtctgggt caaagtcaaa tccggttggg cggacgacat ccgcttcctg
    12001 cgcgagctgg gtttgtagtt tttcttgctg aactttacgc aaatgccgtc cgaacaggtt
    12061 tcagacggca ttttgtttca atcgggaata tctttgttaa aaacgggttg atattatctg
    12121 tgca
//
LOCUS       DAWFBO010000049        12102 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFBO010000049 DAWFBO010000000
VERSION     DAWFBO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.049"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(78..2378)
                     /gene="recQ"
                     /locus_tag="WGO87_000970"
     CDS             complement(78..2378)
                     /gene="recQ"
                     /locus_tag="WGO87_000970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0392345.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMCEGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKQMHGQSGIVYCLSRKKVEDVAQFLCENGLEAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGSEQTDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKIDRFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLTVLGQNAV"
     gene            complement(2443..3225)
                     /gene="trpC"
                     /locus_tag="WGO87_000971"
     CDS             complement(2443..3225)
                     /gene="trpC"
                     /locus_tag="WGO87_000971"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0392346.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVPLEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRTRGVHTFLIGETFMRADDIEAEVGKLF"
     gene            complement(3276..4232)
                     /locus_tag="WGO87_000972"
     CDS             complement(3276..4232)
                     /locus_tag="WGO87_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ0392347.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRDRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQASA
                     AISAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRRTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHAGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            complement(4365..5903)
                     /gene="murJ"
                     /locus_tag="WGO87_000973"
     CDS             complement(4365..5903)
                     /gene="murJ"
                     /locus_tag="WGO87_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0392348.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFFLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            5961..6068
                     /locus_tag="WGO87_000974"
                     /pseudo
     CDS             5961..6068
                     /locus_tag="WGO87_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6362..7060
                     /locus_tag="WGO87_000975"
     CDS             6362..7060
                     /locus_tag="WGO87_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0392349.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(7117..8121)
                     /locus_tag="WGO87_000976"
     CDS             complement(7117..8121)
                     /locus_tag="WGO87_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0392350.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            8191..10599
                     /locus_tag="WGO87_000977"
     CDS             8191..10599
                     /locus_tag="WGO87_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0392351.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            10704..11720
                     /locus_tag="WGO87_000978"
     CDS             10704..11720
                     /locus_tag="WGO87_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0392352.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKVAKVAKVAKVAAT
                     AQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKEAQISESELSR
                     QVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRDFADNIIAEKV
                     RQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAVGAE
                     STIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQ
                     VGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHSGAY
                     VDIR"
BASE COUNT         2807 a         3470 c         3386 g         2439 t
ORIGIN      
        1 cctgattttt gttaatccac tataaaaaca ggaattttta aatagaggca atgccgtctg
       61 aaacttggta acgggcttca gacggcattt tgtcccaaca ccgtcaacac cgccgcaccg
      121 taacgtgcgg ctttttcttc gcctacgcca tatacggcgg caagctccgc caaaccttcc
      181 ggctgtttgg cggcaatggc gcgcagggcg gctttgctga gaatgcggta gggttcggac
      241 tgttcgtgtt ttgccgtttc gccgcaccat tggatcaggg cgcgcatcag gcggcgtttg
      301 cgtttggcgg cttcatcgat gccgtctgaa aacggacggc agacggcgag gatgtcccgt
      361 ccgtatttgg cggcgcgtac gctgcccaag ccgtgtacgc cttcgaggtc ggtttcggtt
      421 tcaggcgtgg cggcaagcat atcggcaagg ctttcgtcgg agaggacggc atgcagggcg
      481 cagttttccg cccttgcctg ttcataccgc caggcttcga gtttttgacg cagttgctgt
      541 tcgcgttcgg tttgcggacg gatgacggca tcgtggctaa agtcggcagc gttttggcag
      601 actttgagta tgccgtgtcc gaaacggtcg attttggctt cgcccaagcc gtagatgtcg
      661 tgcagcccgt tgaggttttg cggcattttt tcgacaaggt cgcgcagggt tttgtcgccg
      721 aaaatcatat aggcggggat gccttcggct tctgcctgtt tcatacgcca aacgcgcaat
      781 gcctgccaca ggcgttcttc gcgttcggta cgcagccagt tgtctttgag ggtgcgggcg
      841 gcgggcttgt cgcgcttgag cggacgcagc atcacttcgg tttcgccttt gaggactttt
      901 ttggcggctt cggtcagttg caatgcctga tatcgggtaa tgttgacggt gagatagccg
      961 aggctgatgc actggcggat gacgctgcgc cattccttgt cggtctgttc gctgccgatg
     1021 ccgaaggtag acagttgttc gtgccggttg ccgcgtatcc aatcatcgct tttaccacgc
     1081 aaaaggttgg tgatgtaacc ggcggcaaaa cgttgtccgg cgcggtacac gcagctgagt
     1141 aatttttgta ccaacaccgt gccgtcaaac cgcacaggcg gatgcaggca gttgtcgcaa
     1201 tggccgcagg gttcggatgc ttcgccgaaa tgtttgagca gcagtacgcg gcggcaggcg
     1261 gcggtttcgc agacggaaag catggcatcg agtttttgca tttcgatttg cttttgcacc
     1321 tcgtcgctgt tgccctcggc aatccgttcg cgcagcaaca cccaatcgtt caaaccgtaa
     1381 cacagccaac tcacggctgg cagcccgtcc cggccggcgc gccccgattc ctgatagaaa
     1441 tgttcgacac tttggggcat atcgagatgg gcgacaaagc gcacgtcggg tttgtctatg
     1501 cccatgccga acgccacggt cgccaccacg ataatattgt cttcatgcgt aaagcggcgt
     1561 tggttttcct cgcgcacgtc catgctcaaa ccggcatgat acggaatcgc ctccaagcca
     1621 ttttcacaca aaaactgcgc cacatcctcg acttttttgc ggcttaggca atacacaatg
     1681 ccgctttgcc catgcatctg cttgcggatg aaatccagca attgtttttt accattattt
     1741 ttttcgataa cctgataata aatattcggg cggtcaaagc tggaaataaa ttcgggcgca
     1801 ttgtccaagt gcagataatg cttgatgtcg gcgcgcgtgg cggcatcggc ggtagcggtc
     1861 agggcgatgc gcgggacgtt cggatagcgt tcggcaagca tgccgagctg ttggtattcg
     1921 gggcggaaat cgtgtcccca ttggctgacg cagtgcgcct cgtcaatggc gaacaggctg
     1981 acggtttgtt ggtcgagaaa acgcaaaaag cggtcggtaa ctaggcgttc cggtgcgaca
     2041 taaagcagtt tcagacgacc ttgggcaagc ttgtcggcaa tctcgcgcgc cccatctgcc
     2101 gatgtgccgc tgttgactgc cgccgcttcg atgccggcgg cgtgcaggtt tgccacttgg
     2161 tcgttcatca acgcaatcag cggcgatacg acaaccgcca cgccttcgca catcagcgcg
     2221 ggaatctggt aacacaaaga cttgccgccg cccgtcggca tcagcaccgt caaactcccg
     2281 ccgcctgcca aagtattgat gacagcctcc tgcctgccgc gaaattcggg ataaccaaat
     2341 acttcgtgca gaatctgttt ggcggtcggt cggtgcatga tggttccgtg ctcggtaagg
     2401 gtgttggtcg gttggcggca atatgccgtc tgaaatcggg atttagaaga gtttgcccac
     2461 ttctgcttca atatcgtcgg cacgcatgaa cgtttcgccg atcaggaagg tgtgcacacc
     2521 gcgcgtccgc ataaattcca catccgcctt acctgtaatg ccgctttcgg taacgacggt
     2581 tttgccttcc agcgcgggca gcagcgacag ggtttggtcg agggagactt caaaagtcct
     2641 caggttgcgg ttgtttacac cccacaacgg cgtggtcagg ttgcggcatt tttccaattc
     2701 gctttcatcg tgcagctcga gcaggacggt catgcccaat tcgtgcgcca ccgcttcaaa
     2761 gcgttccaat tgttcccgtt ccagtgcggc ggcaatcagc aggacggcat ccgcccccca
     2821 ggcgcgcgcc tgataaacct gatattcgtc gatgatgaag tctttgcgca gcacgggcag
     2881 cgatacggct tcgcgcgcct gtttgaggta ttcgggcgaa ccttggaaat agggttcgtc
     2941 ggtcagtacg gacaaacacg ccgccccggc gttttcatag gcgcgggcaa tctcggcagg
     3001 tcggaagtcc ggacggatca ggcctttgct cgggcttgcc tttttgattt cggcaatgac
     3061 ggcgggcagg tttaggcggt gtttgccgcg tatcgaatcg atgaagctgc ggacgggcgc
     3121 ggcttctgcg gcaagtgcgc ggatgtgttc gaggggaacg gcggcttttt gggcggcaac
     3181 ttcctgtgcc ttggcggtaa ggattttatt gaggatgtcg gtcatattgt gttccgtatt
     3241 cgtccgggga aagggggaat attagcatca aaccgttaac gcctgtttgt gcggaagctg
     3301 tcgaaatagg acaggacggt ctgcggcagc cattgcaggt gcagcctgcc gccggtgctg
     3361 ccgacaaagc cggcgtgtcc gccgtgtgcc ggctggaaca gggtaacggc ttcggacact
     3421 tcgtccggtg tcggcagcgc ttcgggcggc aggaaggggt cgttggcggc attgagcagg
     3481 agcagcggtt tggcaacgtg tttgagcagc ggtttgcagg aagttcggcg gtagtagtcg
     3541 tgccggtcgg caaagccgtg cagcggtgcg gtgaagcggt cgtcaaactc gcccagtgtt
     3601 ttgcaccctg cggcaaatgc cgtctgaaaa ccgtggagcg accgtgcttt gggtatcagg
     3661 gtgcggagga agtagcgcgt gtagagcagc cgtgtgatgc cgctgtcgaa gcggctgcct
     3721 gccgcctctg catcgacggg ggcggagatg gcggccgagg cttgcggcag cgcgttttcg
     3781 ccctgttcgc ccaaatattt tgccagcgcg ttgccgccca gcgatacgcc gacggcgtat
     3841 atttcacggt aacgcgcggc gagcgtgtcc aaagtaaagg cgatttcggc ggtatcgccc
     3901 aagtggtaga acactggggc ggtgttcgct acgccgccgc agctgcggaa atggacgact
     3961 acgccgttcc aaccccgatc gcgtaccgca agcatcagtt caaccgcgta atggctgcca
     4021 ctgccgccct ccaacccgtg aaacagcacg accagcggcg catcgggcga aatgccgtct
     4081 gaaaagtcgt aggcgacttt ggttttaccc gtgctgtcgg gaagcagctc gcggcggtat
     4141 gtgggtgcgg ggcgttgcag gaatttggcg gcaatcgtgt cggcattgcc gttgcggagg
     4201 aaaaagggcg tgtccggcgg tgttaaaatc ataaggtatc ggttttcttg ttttcagacg
     4261 gcattgatga tgcggcagcc cgtccggctg gtgcggacgc gggggatgcg cgcccgaata
     4321 taggcgcgga aaaagcgttt tccgaaaaag gatatcggca tcggtcagct ttccacccgt
     4381 ttgaaatggc gcggacggaa gcccaaagcc gccagagatg cgaaatacag tccgccgccg
     4441 acggcaatca ggatgaagag ccgggcggcc ttttgcattc caccggcgtg tgcccagtcg
     4501 aacggcagcc agatttgggc ggcatacagg ccgcctccca tcacggcgag cgagagcagc
     4561 atttttgcta agaacgctgc ccaacccttg ccaggttggt aaataccgtg tctgcgtaac
     4621 aggaagaaca acaatccggc attgatgcac gcgcccagac cgatggcaag cgaaagtccg
     4681 acgtgtttca gtgggccgat aaaggcaagg ttcatcaact gcgtgcagat gagcgtgaag
     4741 atggcgattt tgacgggcgt tttgatgttt tgccgcgcat agaagccggg tgccaacact
     4801 ttaatcataa tcaaaccgat taagccgaaa gaataggcaa tcagcgcgtg ctgcgtcatc
     4861 tgcgcgtcaa acagcgtaaa ttcgcggtac ataaacagtg tcgccaccag cgggaacgac
     4921 aacaccgcca gtccgaccgc cgccggcagc gtcagcagca tacacaggcg caaaccccag
     4981 tcgagcaggg cggaaaactg ttccgtatct tggtttgccg agtgtttgga caaagtcggc
     5041 agcaaaatcg taccgagtgc cgcccccagc acgccgctgg gcagctccat catgcggtcg
     5101 gcgtaataca tccatgaaac gctgcccgat tgcagataag acgcgaaaat cgtgttgatc
     5161 accaaagaaa cctgcgccac gctcacgccc aaaatcgcag gcgccatctg tttcatcacg
     5221 cggttgaccg ccgcatcttt gaaattcagt ttgggcaatt tcaaaaaacc cagtttcgcc
     5281 aaccaaggga gctgaaagcc gagctgcaaa ataccgccga caaaaaccgc ccaagccagc
     5341 gcggtaacgg gaggatcgaa atacggcacg aaaaacagcg cgaatacgat aaacgacacg
     5401 ttcaggaacg tgggcgtaaa cgccggaatg ctgaatttat gataggaatt gagtaccgag
     5461 ccgacaaaag aggaaagtga aatcaataag atatagggga acgtaatccg cagcaaatcg
     5521 atggagagct gaaatttgtc ggcatctttg gcaaaaccgg gtgcggaaac ataaatcacc
     5581 caaggcgcgg caagtatgcc cagcgcggta acgatgacca gcacaaacga cagcatcccc
     5641 gccacatggc ggataaaagc ctccgtcgcc tctttagaac gcgtttcctt atattccgcc
     5701 aaaatcggca caaacgcttg ggcaaacgcc ccctccgcaa acacgcggcg aagcaggttg
     5761 ggcagtttga acgcgacaaa aaacgcatcc gtcgccatgc ccgcgccgaa tgcgcgcgca
     5821 atgaccgtat cgcgcacaaa tcccaaaacg cgcgacacca tcgtcaggct gccgactttt
     5881 gccaaagctc ccagcatatt catcgtattt ccttttcggt tgaaaccccg ccctttaggg
     5941 cggcaggatc agactttgat agtggattaa atttaaatca ggacaaggcg acgaagccac
     6001 agacagtaca aatagtacgg caaggcgagg caacgctgta ctggtttaaa tttaatccac
     6061 tatatttgaa aggggtgtaa cccttccgaa ccagggcaac gcatagggcg gtgctttatg
     6121 tgccgtcctg tgcgttggaa catcattagt tccccaacag ccgtatccgt ctggggcgac
     6181 ggcgcgtatt gtacgacaga aaccgcttca gacggcatcg ggtttgatgc cgtccgaagc
     6241 ggtttcccca aacgaaaacg tccttttccg gcggcaaact gtatcaacac gcggaaatgc
     6301 aataaaatag ccggattccg attgatttcc aacatctgtt tccaacatca cggagagccg
     6361 tatgaaatcc agacacctcg ccctcggcgt tgccgccctg ttcgcccttg ccgcgtgcga
     6421 cagcaaagtc caaaccagcg tccccgccga cagcgcgcct gccgcttcgg cagccgccgc
     6481 cccggcaggg ctggtcgaag ggcaaaacta taccgtcctt gccaacccga ttccccaaca
     6541 gcaggcaggc aaagtcgaag tccttgagtt tttcggctat ttttgtccgc actgcgccca
     6601 cctcgaacct gttttaagca aacacgccaa gtcttttaaa gacgatatgt acctgcgtac
     6661 cgaacacgtc gtctggcaga aagaaatgct gacgctggca cgcctcgccg ccgccgtcga
     6721 tatggctgcc gccgacagca aagatgtggc gaacagccat attttcgatg cgatggtcaa
     6781 ccaaaaaatc aagctgcaaa atccggaagt cctcaaaaaa tggctgggcg aacaaaccgc
     6841 ctttgacggc aaaaaagtcc ttgccgccta cgagtccccc gaaagccagg cgcgcgccga
     6901 caaaatgcag gagctgaccg aaaccttcca aatcgacggt acgcccacgg ttatcgtcgg
     6961 cggtaaatat aaagttgaat ttgccgactg ggagtccggt atgaacacca tcgacctttt
     7021 ggcggacaaa gtacgcgaag aacaaaaagc cgcgcagtaa gcccgtttga aaaatgccgt
     7081 ctgaaacttg gttttcagac ggcattttga ttgggtttaa aacgtaaagc ccgtttccag
     7141 ttcttcatcg ccgaccagtt cgaccaagag cgcgtagagc ggggcgagtt cggcataacg
     7201 gcgcgatacg cggcgcagat agtttaagaa acgcgggatt tcgggacggt atttgtcttt
     7261 gccgtcacgg tagtacaggc gtgcgaagat gcctgcaacc ttcaagtgcc gctgcacgcc
     7321 catccattcg aaccagcggt aaaactcgtc aaacgcttcg gggacgggca agccggcagc
     7381 ccgcgccttt tcccagtagc ggataaccaa gtccaagaca aattcttctt cccattcgat
     7441 aaaggcatcg cgcaacagcg acaccaaatc gtaggaaatc gggccgtaaa gcgcgtcttg
     7501 gaagtctaaa acgcccggtc tgccgcgcgt cagcatcagg ttgcggacga taaagtcgcg
     7561 gtgcacatag actttgggct gcgccaacag gggcggcagc agcgtatcga cggtttgctg
     7621 ccaaagttgg cgttgtttga atgttaattc gcgccccaat tcttttgcga caaaccattc
     7681 cgggaacagg ttgatttcgc gcagcatcgt ttcacggtca tattcgggca aaaccccttc
     7741 acggcttgcc ttctgcaatt cgaccagctc gccgattgcc tccaaaagca gggctttgtg
     7801 cgccgcttcg ccctgctcct gaagcatcgc ggtcaaaaac gtcgtattgc ccaagtcgtt
     7861 caataccaca aaccccaaat ccgtgtccgc gtgcaatacc tgcggtacat tgaccatgtc
     7921 aaacagtttc tgcactttca aataaggtgc gacactcatc ttgtcgggcg gtgcatccat
     7981 gcagacgaca ctgctgccgt ctgaaaacgt tgcacggaaa tagcggcgga aatcagcatc
     8041 cgccgccgcg aaggacagat cgaagtcccg ttcgggatag atggtctgaa gccaattttt
     8101 cagttcggtt tgccgttgca taacagtact aaagcatttc aggttacaat aaacgctatt
     8161 ctaactggca aaccgacttg aggggcgatt ttggctcgtt tattttcact caaaccactg
     8221 gtgctggcat tgggcttctg tttcggcacg cattgcgccg ccgccgatgc cgttgcggcg
     8281 gaggaaacgg acaatccgac cgccggagga agcgttcgga gcgtgtccga acccatacag
     8341 cctaccagcc tgagcctcgg ttcgacctgc ctgttttgca gtaacgaaag cggcagcccc
     8401 gagagaaccg aagccgccgt ccgaggcagc ggcgaagcat ccatccccga agactatacg
     8461 cgcattgttg ccgacaaggt ggaaggacag tcgcaggtgc aggtgcgtgc cgaaggcaac
     8521 gtcgtcgtcg aacgcaaccg gacgaccctc aataccgatt gggcggatta cgaccagtcg
     8581 ggcgacaccg ttaccgcagg cgaccggttc gccctccaac aggacggtac gctgattcgg
     8641 ggcgaaaccc tgacctacaa tctcgagcag cagaccgggg aagcgcacaa cgtccgtatg
     8701 gaaaccgaac acggcggacg gcggctgcaa agcgtcagcc gcaccgccga aatgttgggc
     8761 gaagggcatt acaaactgac ggaaacccaa ttcaacacct gttccgccgg cgatgccggc
     8821 tggtatgtca aggccgcttc cgtcgaagcc gatcgggaaa aaggcatagg cgttgccaaa
     8881 cacgccgcct tcgtgttcgg cggcgttccc attttctaca ccccttgggc ggacttcccg
     8941 cttgacggca accgcaaaag cggcctgctt gttccctcac tgtccgccgg ttcggacggc
     9001 gtttcccttt ccgttcccta ttatttcaac cttgccccca atctcgatgc cacgttcgcg
     9061 cccagcgtga tcggcgaacg cggcgcggtc tttgacgggc aggtacgcta cctgcggccg
     9121 gattatgccg gccagtccga cctgacctgg ctgccgcacg acaagaaaag cggcaggaat
     9181 aaccgctatc aggcgaaatg gcagcatcgg cacgacattt ccgacacgct tcaggcgggt
     9241 gtcgatttca accaagtctc cgacagcggc tactaccgcg acttttacgg caacaaagaa
     9301 atcgccggca acgtcaacct caaccgccgt gtatggctgg attatggcgg cagggcggcg
     9361 ggcggcagcc tgaatgccgg cctttcggtt ctgaaatacc agacgctggc aaaccaaagc
     9421 ggctacaaag acaaaccgta tgccctgatg ccgcgccttt ccgccgattg gcgcaaaaac
     9481 accggcaggg cgcaaatcgg cgtgtccgca caatttaccc gcttcagcca cgacagccgc
     9541 caagacggca gccgcctcgt cgtctatccc gacatcaaat gggatttcag caacagctgg
     9601 ggttacgtcc gtcccaaact cggactgcac gccacctatt acagcctcaa ccgcttcggc
     9661 agccaagaag cccgacgcgt cagccgcact ctgcccattg tcaacatcga cagcggcgca
     9721 acttttgagc ggaatacgcg gatgttcggc ggcggagtcc tgcaaaccct cgagccgcgc
     9781 ctgttctaca actatattcc tgccaaatcc caaaacgacc tgcccaattt cgattcgtcg
     9841 gaaagcagct tcggctacgg gcagctcttt cgcgaaaacc tctattacgg caacgacagg
     9901 attaacaccg caaacagcct ttccgccgcc gtgcaaagcc gtattttgga cggcgcgacg
     9961 ggggaagagc gtttccgcgc cggcatcggt cagaaattct atttcaagga tgatgcggtg
    10021 atgcttgacg gcagcgtcgg caaaaaaccg cgcaaccgtt ccgactgggt ggcatttgcc
    10081 tccggcagca tcggcagccg cttcatcctc gacagcagca tccactacaa ccaaaacgac
    10141 aaacgcgccg agaactacgc cgtcggtgca agctaccgtc ccgcacaggg caaagtgctg
    10201 aacgcccgct acaaatacgg gcgcaacgaa aaaatctacc tgaagtccga cggttcctat
    10261 ttttacgaca aactcagcca gctcgacctg tccgcacaat ggccgctgac gcgcaacctg
    10321 tcggccgtcg tccgttacaa ctacggtttt gaagccaaaa aaccgataga ggtgctggcg
    10381 ggtgcggaat acaaaagcag ttgcggctgc tggggcgcgg gcgtgtacgc ccaacgctac
    10441 gttaccggcg aaaacaccta caaaaacgct gtctttttct cacttcagtt gaaagacctc
    10501 agcagtgtcg gcagaaaccc cgcagacagg atggatgtcg ccgttcccgg ctacatcccc
    10561 gcccactctc tttccgccgg acgcaacaaa cgaccctgac cgtcggaaac ccggcaggag
    10621 caccgttccc gcacaagacg gcattccacc gacaacccca aacccgccat caaaggtagg
    10681 attcaaacga taaggaaaga atgatgaaaa tcaaagccct gatgattgcc gccgcattgc
    10741 tggcagcagc cgatgtccac gccgcaccgc aaaaggcaaa aaccgcatcc gccaaagttg
    10801 ccaaagttgc caaagttgcc aaagttgccg ccacggcgca aaaagaagcc gcacccgcac
    10861 aacagcaggg cggtatccgc ttttcagacg gcattgccgc cgttgccgac aacgaagtca
    10921 tcacgcgccg ccggcttgcc gaagccgttg ccgaagccaa agccaacctg cccaaagagg
    10981 cgcagataag cgaatccgag ctgtcccgac aggtgctgat gcagcttgtc aaccaatccc
    11041 tgattgtaca ggcgggcaaa cgccgcaaca ttcaagcaag cgaagcggaa atcgatgccg
    11101 tcgtcgcaaa aaattccgcc ctcaaaaacc tcagccccgc ccaacgccgc gattttgccg
    11161 acaacatcat tgccgaaaaa gtccgccagc aggcagtgat gcagaacagc cgcgtgagcg
    11221 aagctgaaat cgatgccttc ctcgagcagg cgcaaaaaca aggcatcacc ctgcccgaag
    11281 gcgcaccgtt gcgccaatac cgcgcccaac acatcctgat taaagccgac agcgaaaacg
    11341 ccgccgtcgg cgcggaaagc accatccgca aaatctacgg agaggcccgc agcggcacag
    11401 acttttccag cctagcgcgc caatattcgc aagacgcgag cgcgggcaac ggcggagatt
    11461 tgggctggtt tgccgacggc gtgatggttc ccgcctttga agaagccgtc cacgcgctca
    11521 aacccggaca ggtcggcgcg cccgtccgca cccaattcgg ctggcatatc atcaaattga
    11581 acgaagtgcg cgatgccggc acacctcagg aacgtatccg caattccgtg cggcaataca
    11641 tcttccaaca aaaagccgaa caggcaaccg tcaacctgtt gcgtgacctg cattccggcg
    11701 cgtatgtcga catccgctaa ggcggtttga agcaaaaagc cataccgatc ggcaaaaatc
    11761 cgggcggtat ggctttttgg atttcgagtt acttttacac cgtcattcat cattcccgcg
    11821 aaagcgggaa tctagaaacg aaaagtaaca ggaatttatc gggaatggct ggagtttaaa
    11881 ggactggatt cccgccgtcg cgggaatgac gggattttgg gttgtggtaa tttatcggaa
    11941 aaacaaaaaa acctatgccg tcattcccga gcaggcggga atccggttat ttaaaactgc
    12001 agaaatttat ccgaagcaac aacaatcttt ccatcgtcat tcccgcgtag gcgggaatct
    12061 agcaacgaaa agtaacagga atttatcggg aaaacagaaa cc
//
LOCUS       DAWFBO010000050        12005 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFBO010000050 DAWFBO010000000
VERSION     DAWFBO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12005
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.050"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..658
                     /locus_tag="WGO87_000979"
     CDS             <1..658
                     /locus_tag="WGO87_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0392353.1"
                     /translation="CDLWTNINTTGLEAEGNVELKNGKKPESLIETIIKLATNENDIV
                     LDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERMKKVIEGEQGGISKAVNWQGG
                     GEFVYAELAPFNETAKQQILACENSDGIKMLFEGLCERYFLKYNVSVNEFGQIIQEPE
                     FQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSRAFYQAEKKDGE
                     "
     gene            648..3428
                     /locus_tag="WGO87_000980"
     CDS             648..3428
                     /locus_tag="WGO87_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0392354.1"
                     /translation="MANNKTLFEVIENRKAVYLEDGDDEKCRLPEFVERNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDQVIPIRKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQGELDLEKEIND
                     RTSNAEIERKGWEHMVLELLLNKNGNPSENVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENIQADNFSFLTTFWASLNDSDNANEQGKTRTEQALKFMQDNK
                     FGFAHLANWVKQNYQKHNIIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFAANKDFRELLIWANKKIPNPNAKSNNADSLKANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLD
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVKDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(3572..4744)
                     /locus_tag="WGO87_000981"
     CDS             complement(3572..4744)
                     /locus_tag="WGO87_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0392355.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            5094..5540
                     /gene="iscR"
                     /locus_tag="WGO87_000982"
     CDS             5094..5540
                     /gene="iscR"
                     /locus_tag="WGO87_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0392356.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            5569..6783
                     /locus_tag="WGO87_000983"
     CDS             5569..6783
                     /locus_tag="WGO87_000983"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0392357.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7047..7433
                     /gene="iscU"
                     /locus_tag="WGO87_000984"
     CDS             7047..7433
                     /gene="iscU"
                     /locus_tag="WGO87_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0392358.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKGDDSVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            7520..7840
                     /gene="iscA"
                     /locus_tag="WGO87_000985"
     CDS             7520..7840
                     /gene="iscA"
                     /locus_tag="WGO87_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0392359.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(7800..8021)
                     /locus_tag="WGO87_000986"
     CDS             complement(7800..8021)
                     /locus_tag="WGO87_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0392360.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            8103..8603
                     /gene="hscB"
                     /locus_tag="WGO87_000987"
     CDS             8103..8603
                     /gene="hscB"
                     /locus_tag="WGO87_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0392361.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            8701..11451
                     /gene="gyrA"
                     /locus_tag="WGO87_000988"
     CDS             8701..11451
                     /gene="gyrA"
                     /locus_tag="WGO87_000988"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0392362.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            11608..12005
                     /locus_tag="WGO87_000989"
                     /pseudo
     CDS             11608..12005
                     /locus_tag="WGO87_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3592 a         3045 c         2700 g         2668 t
ORIGIN      
        1 atgcgattta tggacaaata taaatacaac aggactagaa gcagaaggaa atgtagagtt
       61 aaaaaatggt aaaaagccag aaagtttaat tgaaactatt attaaactag caaccaacga
      121 aaacgacatc gtcctagact accatttagg tagtggcaca accgctgccg ttgctcacaa
      181 aatgaaccgc caatatattg gtattgaaca aatggattat attgaaacgc ttgccgttga
      241 acgcatgaaa aaagtgattg aaggcgagca aggtggtatt tccaaagccg tgaattggca
      301 aggtggtggc gagtttgtct atgccgagct tgctccattt aacgaaaccg caaaacaaca
      361 aattttggct tgcgaaaatt cagacggcat caaaatgctg tttgaaggtt tatgcgaacg
      421 ctatttccta aaatacaatg tgagcgtaaa tgagtttggt caaatcattc aagagcctga
      481 atttcaatct ttgccattag acgaacaaaa acaaatggtg cttgaaatgt tggatttaaa
      541 tcaaatgtat gtttcattat ccgaaatgga cgatgaacaa tttgcaggct gcttgaatga
      601 tgatgataaa gccttaagcc gtgcgttcta tcaagcggag aaaaaagatg gcgaataata
      661 aaactttgtt tgaagtgatt gaaaaccgca aagctgtgta tttggaagac ggcgatgatg
      721 aaaaatgcag actgcctgaa tttgtagaac gcaatttaaa atatccgttt tttgaatggc
      781 aaaaatcggc tttagaaaat tttgtgattt ttgaccgcac ttcaaaatta aaggatttcc
      841 ctgacatcaa aaaccgccct acccatttgc tgttcaatat ggcaacaggt gcaggcaaaa
      901 cgatgatgat ggcggcgttg attttgtatt attttgaaaa gggttatcgg cattttttgt
      961 ttttcgttaa tcaaaataac atcgtggata aaacggaaaa taattttatt gacccggcgc
     1021 acgcaaaatt tttatttacc gagaagattt tgcaaggcga ccaagttatt cccattcgca
     1081 aagtagaaac gtttagccca cattcagacg gcattgaaat taaatttacc agtattcaaa
     1141 aactgtataa cgatatccac accgaacggg aaaaccaaac cacattggcg gatttgcaca
     1201 aattgaacct tgtgatgctg ggcgatgaag cacaccattt aaacgcacaa accaaaggca
     1261 aaaaacaagg cgaattagat ttagaaaagg aaattaacga ccgcactagc aatgccgaga
     1321 ttgaacgcaa aggctgggag catatggttt tggaattgtt actcaataaa aatggcaatc
     1381 ctagcgaaaa tgtgctgttg gaatttaccg ccacgctacc tgaaaatgct gacgtgcaac
     1441 aaaaatacgc ggataaaatc atcacaaaat ttggcttaaa agagtttttg caaaaaggtt
     1501 ataccaaaga aatcaatttg gtatccagta cgctgggcaa gaaagagcgg gtgttgcacg
     1561 ctttattgtt tgcttggtat cgccatcaaa tcgcgttgaa atacggcatt gccaatttca
     1621 agcctgtgat gttgtttaga agtaagacga ttgatgaatc aaaagcggat tattcggcgt
     1681 ttttaaattg ggcagaaaat atacaggcgg ataatttttc gtttttaacg acattttggg
     1741 caagcttaaa cgatagcgat aacgccaacg aacaaggtaa aacccgcacc gaacaagccc
     1801 taaaatttat gcaggacaat aaatttgggt ttgcacattt agcaaattgg gtaaaacaaa
     1861 attatcaaaa acacaatatc attattacca actccgaaac caacaaaacc aaaaccgaaa
     1921 aaaccgacag cgaaacggaa aaattgctga ataatttgga agcggctgat aatccgatcc
     1981 gtgccatttt tacggtggat agattaactg aaggttggga cgttctgaat ttgtttgata
     2041 ttgtgcgttt gtatgaaggg caaaatggcg gtggttcaaa taaaaaatca ggcaaaacgg
     2101 ctgccgctac cgtatcggaa aagcagttga ttggtcgtgg cgtgcgttat tttccatttg
     2161 cgtttgaagg taaacagccg aataaacgca aatttgacaa cgacatgcaa cacgaattgc
     2221 gtatcttgga agaattgttt tattacacgc acgatgagca atcccgctat atttcagaac
     2281 tgaaaaatga gttgcgaaaa gacggttatt tgcctgaaaa agacgatgat aaggtattgg
     2341 caacatttaa actcaaatct gaatttgccg ctaataagga ttttagagaa ttgttaattt
     2401 gggcgaataa aaaaatcccc aacccaaacg ccaaaagcaa taatgcagac agcctaaaag
     2461 ccaatccaca aacgctttca ttccaagttc acggtaatca actgttgcag gaaacgcaat
     2521 ttacagccga tgaaaatgat gaaacagccc gacaaatcgg cacacaaaat aattttactc
     2581 aaaccataaa aatgagtgaa atggagcggc acattttcaa taaagcatta catatcaaag
     2641 ggaaaaatag tcaatcttta ttccatttca accgtttgca aagcaagctc gacattcaaa
     2701 atcgcaatga attgcaaaat aacttgttga aagactggca aattgaattt ttaggattag
     2761 ggcaagacaa acaggttcgc ctggacgata aacttgcagg ctgcctgaaa atcttggaaa
     2821 tggttgaaaa acatctgaat gaaagtgata tgccgtttat cggtacaaaa gaatttacac
     2881 ctaaaaaatt atgggaaatt ttcggcacgc caaaacaaaa atgggtcaaa aaagatgatg
     2941 taaaaacgac tattgccacg caaaatgatt ggtatgtgat gaataatttt gccggaacgg
     3001 gtttggaaga agcgttaatt caatttattt cagcgcgttt gggcgatttg aaatctcaat
     3061 atgacattca tttaatccgt aatgaagaag tgtttaaatt gaataacttt gccgatggtg
     3121 aaggatttat gccggacttt attttattgc tgaaagataa acaaaaatct tcttccaatg
     3181 gcgtgaagga ctttttgcat taccaaattt tcattgagcc caaaggtgag catttagtgg
     3241 aaactgatgg atggaaagaa gaatttttag aagccatcac tgtggaatat gggatggata
     3301 agatcctgca aaaagataca ccgcattatc gtttgatcgg tttaccgttt tttactgatc
     3361 atcagaaaaa tggacaattt acggagtcat tccctttagg tgcggcatcg cttgaaaaaa
     3421 tgaagtagtg cattgcaggc aaccccgttt gacaaaactt cctttacaaa agggcgtttt
     3481 gtcagatatt taatcaacac attattaaaa tacagccaaa ttttaatgcc gtccgaaccc
     3541 tgtgttcaga cggcatcgta tttttcagta tctaaaccgt ttccctgccc caatctttgc
     3601 ctctcaaaat cgaagcatcg acatcttgaa tatcgcggtg tcccgtaaac gccatagata
     3661 tatccatttc tttatacagg atttccagcg cacgggttac gccttcttca ccatacgcac
     3721 ccaaaccata cagaaacgcc cgacctatca ttgtaccttt cgcgcccaaa gcccacgcct
     3781 tcaaaatatc ctgaccgctg cggatgccgc tgtccatcca aacttcgatg tcgctgccca
     3841 ctgcgctgac gatgtcgggc aaggctttga tggcagacac ggtatcgtcg agctgtcggc
     3901 cgccgtggtt ggaaacaatc aatgcgtccg cgccgctttt cgctgctttt tccgcgtctt
     3961 cgggttccat aatgcctttg ataatcagct tgccgcccca caaatcttta atgcgcgcca
     4021 catcgtccca gctcaggcgc gggtcgaatt gttcggaagt ccatgaagac agcgaagaca
     4081 aatcgccgac gttcttcgcg tgtccgacga tattgcggaa cgtgcggcgt tccgtgttca
     4141 gcattttcat gcaccattcc ggtttggtcg ccagattgat taaattggcg atggtcggtt
     4201 tcggcggcgc ggacaggccg tttttgatgt ctttgtggcg ttgacccaaa acctgcaaat
     4261 cggcggtcaa taccaatgcc gaacatttgg catccttcgc gcgcttaatc aggttttcca
     4321 taaactcgcg gtcgcgcatc acataaagct gaaaccaaaa tggtgcggaa gtgttctcgg
     4381 caacgtcttc aatcgagcag atagacatcg tggacaacgt aaacggaatg ccgaacttct
     4441 ccgccgcccg cgccgccaag atttcaccgt cggcgtgtgc cataccggta aaacccgtcg
     4501 gcgcaatcgc caccggcatt ttcacatcct gcccgatcat tttggtttcc aggcttcgac
     4561 cttccatatt gaccaatacc ttttgtcgga agcggatatc tttaaaatcc gaagtgtttt
     4621 cgcggtaggt agtttccgtc cacgaacccg aatcgatgta atcgtaaaac atacgcggca
     4681 ttttgcgctt ggcaacgcgg cgcaagtctt cgatgcaggt cattttgctc aaatcacgtt
     4741 tcatcttgtt tttccctgtt tgacagcctg atggaatatg ccgtccgaaa ccgcttgagc
     4801 cttcagacag catttcaggc aatacccaaa taaatcacta agatttagat aatctatatc
     4861 aatatcaaaa ataaatcaaa tcgttttgaa accgcttgcc ggcaaaatac cccgaccacc
     4921 gctcctcaaa aaacataacc aactgattta aaaaaccaaa caaataaagc aaaatcagac
     4981 tataccgcca tttaaacaaa caatcggcaa cagctccccc ttacttgact gaaacactca
     5041 gatattggac aattctcccc actataaaaa agccgacacg ggcaaccacc accatgagac
     5101 tgaccaccaa agggcgtttt gccgttaccg ctatgctgga tttggcgatg aacgcgcaaa
     5161 ccggcgccgt caaactcagt gccatcagcg aacgccaaaa catatccctc tcctatctcg
     5221 agcaattgtt cggcaaactc cgccgcgccg gactggttga aagcctgcgc gggcccggcg
     5281 gcggctacat cctcgccgca ccggcggcac gcatcaacat cgcccaaatc atcgccgccg
     5341 ccgaagaccg gctggacgcg acccaatgcg gcagcaaagc caactgccac cacggcgcgc
     5401 cctgcctgac gcacgatctt tgggagaatt taaacaaaac catcaacgac tacctcggca
     5461 gcgttaccct gcaaagcatc atcgaacaga aaaacaacgg cgacggcagc cgcgtcgtcc
     5521 aatttacaca catccattaa ataacacccg aaaaagaaag agcaaaccat gaccgtcaaa
     5581 acccccgttt acctcgacta cgccgccacc acccccgttg acaaacgcgt tgccgaaaaa
     5641 atgattccct atctgaccga aaccttcggc aacccagcct ccaacagcca cagcttcggc
     5701 tgggaagcag aagaagctgt agaaaaagca cgtgcagaca ttgccgccct gattaacgcc
     5761 gactctaaag aaatcgtttt caccagcggc gcaaccgagt ccaacaacct cgctatcaaa
     5821 ggcgcggcgc acttctacaa atctaaaggt aatcacctca tcactgtaaa aaccgaacac
     5881 aaagccgtac tcgacaccat gcgcgaactc gaacgccaag gttacgaagt aacttatctg
     5941 gacgtacaag aaaacggttt ggttgattta gacgtactga aagccgccat ccgcgaagac
     6001 accatcctcg tttccgtaat gtgggtaaac aacgaaatcg gcgtggttca agatattcct
     6061 gccatcggcg aaatctgccg cgaacgcaaa atcattttcc acgttgacgc agcacaagca
     6121 tgcggcaaag tgcctgtcga tgtcgaagcc gccaaaatcg acctgctctc gatgtccgcg
     6181 cacaaagtgt acggccccaa aggcatcggc gcgctgtacg tccgccgcaa accccgcgtc
     6241 cgcctcgaag cccaaatgca cggcggcggc cacgaacgcg gcttccgcag cggcacattg
     6301 ccgacccacc aaatcgtcgg tatgggcgaa gccttccgca tcgccaaaga agaactcgaa
     6361 caagatatgg cgcactaccg caaactgcgc gacatcttcc tcaaaggcat cgaaggcatc
     6421 gaagaagtct atgtcaacgg cgacctcgaa caccgcgccc cgaacaacct gaacgtcagc
     6481 ttcaacttcg tcgaaggcga aagcctgatt atggcggtga aagaactcgc cgtatccagc
     6541 ggctccgcct gtacttctgc cagtctcgaa cccagctacg tcctgcgcgc gctcggccgc
     6601 aacgatgaac tggcgcactc atccctgcgc atcaccttcg gtcgcatgac caccgaagaa
     6661 gaagtgcaat tcgccgccga actgattaaa tccaaaatcg gcaaactgcg cgaactgtcg
     6721 ccgctgtggg aaatgttcaa agacgggatt gatttgaact cgattgaatg ggcagcgcat
     6781 taaagcgtac caacatgccg tccgaacctt tagacggcat tccaaaaaca aagcaatcaa
     6841 gagaaaatat gaacgaacaa gatttagatt tggacaatct cgacaacctg cttgaagatt
     6901 ttgacggcgt taccgtggaa ggcggcgtcg attcggaaaa cgacgacggc tgcgaaggcg
     6961 gggcgtgcaa aatctaaact tcaggccgtc tgaaaatcgg caaacaaacc acttaaacca
     7021 tcaaaaaaca ttaaggaaac cacatcatgg catacagcga taaagtaatc gaccactatg
     7081 aaaatccgcg caacgtcggc acattcgaca agggagacga ttccgtcggc accggcatgg
     7141 tcggcgcgcc cgcctgcggc gacgtcatgc gcctgcaaat caaagtgaac gacgagggca
     7201 tcatcgaaga tgcgaaattt aaaacttacg gctgcggctc ggccatcgct tcgtccagcc
     7261 tgattaccga gtgggttaaa ggcaaaagcc tggatgacgc gctggcaatc aaaaacagcg
     7321 aaatcgccga ggagttggaa ttgccgccgg taaaaatcca ctgctccatc ttggctgaag
     7381 atgcggtaaa agcggccgtt gccgactacc gcaaacgtca ggaaaacaga taaagccctt
     7441 cagacggcat catcccgcaa tgccgtccga accacccgcc gcttcaggtc cgtccggggc
     7501 ggtacaacaa ggaagaaata tgattaccct taccgagaat gccgcaaaac acatcaatga
     7561 ctatctcgcc aaacgcggca aaggcttggg cgtacgcttg ggtgtgaaaa ccagcggctg
     7621 ctcggggatg gcgtacaacc ttgaatttgt cgacgaagcc gatggcgacg acctgatttt
     7681 cgaaggacac ggcgcgcgca tttatatcga tccgaaaagc ctggtttatc tggatggcac
     7741 gcaagtcgat tacaccaaag aaggtttgca ggaaggtttc aaatttgaaa accccaatgt
     7801 caaagactcc tgcggctgcg gcgaaagctt ccacgtttaa ggcataaaaa cggcgggacc
     7861 gtatcaaaac cgtcccgcca ttttttcgct tcctgcctgt tgtagctgcc tttgcctttc
     7921 cttttccgtt ccaccttgtg ccggaacaaa tcggatttca ctaaggcttt taaagcattg
     7981 tcgcgtattt tgcctttatt gtgctgcact ttgccgccca tattcagtcc tttcgtttaa
     8041 gaagcggcag attataaggc aaaaacagtt ttctgccaaa atcttacatt tatcatccta
     8101 ctatgtccca atatttcacc ctcttccgga ttgaacccgc tttcgatatc gacaccgaaa
     8161 acttggaaca aacctaccgc gccttggccg cccgtttcca tcccgataaa ttcgcttcag
     8221 cttccgcctt tgagcaaaag caggcagtga tgatgtcttc caccatcaac gatgcctacc
     8281 gcaccttgaa aaaccccatc gaccgcgccg cctacctgct gaaaacatcg ggcatcgatg
     8341 ccgacgcgcc ggagcatacc gctttcgccc ccgaattcct tatgcagcaa atggaatggc
     8401 gcgaaacgct gatggaggca cgggcaggca acgaccttga atccttgaaa aatctcgaca
     8461 acgaaatccg cgacgaacaa gaaaaactgt tctgcggtct gaaacagtcg tttgcccgac
     8521 aagattacga cacagccgca caacaagtcc gccaaggcag gtttctcgac aaactccgca
     8581 acgaaatttc ctcggcatta taatccgcac cgtgtttcag acggcgtaac cgccgcaccg
     8641 ttccgcgtca aaatatgcta aaataagcaa caattttttg ccatacgaaa cattgaaacc
     8701 atgaccgacg caaccatccg ccacgaccac aaattcgccc tcgaaaccct gccggtaagc
     8761 cttgaagacg aaatgcgcaa aagctatctc gactacgcca tgagcgtcat tgtcgggcgt
     8821 gcgctgccgg acgttcgcga cggtctcaag ccggtacacc gccgcgtact gtacgcgatg
     8881 cacgaattga aaaacaactg gaatgccgcc tacaaaaaat cggcgcgcat cgtcggcgac
     8941 gtcatcggta aataccaccc ccacggcgat accgccgtat acgacaccat cgtccgtatg
     9001 gcgcaaaatt tcgctatgcg ttatgtgctg atagacggac agggcaactt cggatcggtg
     9061 gacgggcttg ccgccgcagc catgcgctac accgaaatcc gcatggcgaa aatttcccac
     9121 gaaatgctgg cagacattga ggaagaaacc gtcaatttcg gcccgaacta cgacggtagc
     9181 gaacacgagc cgcttgtact gccgacccgt ttccccacac tgctcgtcaa cggctcgtcc
     9241 ggcatcgccg tcggcatggc gaccaatatc ccgccgcaca acctttccga taccgtcaat
     9301 gcctgcctgc gcctgctcga tgcacccgac accgaaatcg acgaactgat cgacattatc
     9361 caagcccccg acttcccgac cggggcaacc atctacggct tgagcggcgt gcgcgaaggc
     9421 tataaaacag gccgcggccg cgtcgttatg cgcggtaaga cccatatcga acccataggc
     9481 agaaacggcg aacgcgaagc catcgttatc gacgaaatcc cctatcaggt caacaaagcc
     9541 aagctggtcg agaaaatcgg cgatttggtt cgggaaaaaa cactggaagg catttccgag
     9601 ctccgcgacg aatccgacaa atccggtatg cgcgtcgtta tcgagctgaa acgcaacgaa
     9661 aatgccgaag tcgtcttaaa ccaactctac aaactgactc cgctgcaaga cagtttcggc
     9721 atcaatatgg tggttttggt cgacggacaa ccgcgcctgt tgaacctgaa acagattctc
     9781 tccgaattcc tgcgccaccg ccgcgaagtc gttacccgac gtacgctttt ccggctgaag
     9841 aaggcacgcc atgaagggca tatcgccgaa ggcaaagccg tcgcactgtc caatatcgat
     9901 gaaatcatca agctcatcaa agaatcgccc aacgcagccg aggccaaaga caaactgctt
     9961 gcgcgccctt ggcgcagcag cctcgttgaa gaaatgctga cgcgttccgg tctggatttg
    10021 gaaatgatgc gtccggaagg attggctgca aacatcggct tgaaagagca aggttattac
    10081 ctgagcgaga ttcaggcaga tgctatttta cgcatgagcc tgcgaaacct gaccggcctc
    10141 gatcaagaag aaattgtcga aagctacaaa aacctgatgg gtaaaatcat cgactttgtg
    10201 gatatcctct ccaaacccga acgcattacc caaatcatcc gcgacgaact ggaagaaatc
    10261 aaaaccaact atggcgacga acgccgcagc gaaatcaacc cgttcggcgg cgacattgcc
    10321 gatgaagacc tgattccgca acgcgaaatg gtcgttaccc tgacacatgg cggctatatc
    10381 aaaacccagc cgaccaccga ctatcaggcg cagcgtcgcg gcgggcgcgg caaacaggcg
    10441 gctgccacca aagacgaaga ctttatcgaa accctgtttg ttgccaacac gcatgattat
    10501 ttgatgtgct ttaccaattt gggcaagtgt cattggatta aggtttacaa actgcccgaa
    10561 ggcggacgca acagccgcgg ccgtccgatt aacaacgtca tccagttgga agaaggcgaa
    10621 aaagtcagcg cgattctggc agtacgcgag ttccccgaag accaatacgt cttcttcgcc
    10681 accgcgcagg gaatggtgaa aaaagtccaa ctttccgcct ttaaaaacgt ccgcgcccaa
    10741 ggcattaaag ccatcgcgct caaagaaggc gactacctcg tcggcgctgc gcaaacaggc
    10801 ggtgcggacg acatcatgct gttctccaac ttaggtaaag ccatccgctt caacgaatac
    10861 tgggaaaaat ccggcaacga cgaagcggaa gatgccgaca tcgaaaccga aatttcagac
    10921 ggcatcgaag atgaaaccgc cgacagcgaa aacgcactgc cgagcggcaa acacggtgtt
    10981 cgcccgtccg gtcgcggcag cggcggtttg cgcggtatgc gcctgcctgc cgacggcaaa
    11041 atcgtcagcc tgattacctt cgcccctgaa accgaagaaa gcggtttgca agttttaacc
    11101 gccaccgcca acggatacgg aaaacgcacc ccgattgccg attacagccg caaaaacaaa
    11161 ggcgggcaag gcaatattgc cattaacacc ggcgagcgaa acggcgattt ggtcgccgca
    11221 accttggtcg gcgaaaccga cgatttgatg ctgattacca gcggcggcgt acttatccgc
    11281 accaaagtcg aacaaatccg cgaaaccggc cgcgccgcag caggcgtgaa actgattaac
    11341 ttggacgaag gcgaaacctt ggtatcgctg gaacgtgttg ccgaagacga atccgaactc
    11401 tccgacgctt ctgtaatttc caatgtaacc gaaccggaag tcgagaactg aaaatcatct
    11461 cccgatgccg tctgaagatt cagacggcat ttattttatc cctcatccgt catccagctt
    11521 ctcacaatat agcggattat agtcaattaa aaacaagggg ctgtcctaga taactaggga
    11581 aattcaaatt aagttagagt tgcccctatg agaaaaagtc gtctaagccg gtataaacaa
    11641 ataaactcat tgaactgttt gtcgcaggtg taactgcaag aacgacagca gagttagtag
    11701 gcgttaataa aagtaccgca gcctattatt ttcatcgttt acgattactt atttatcaaa
    11761 acagtccgca tttggaaatg tttgatggcg aagtagaagc agatgaaagt tattttgctg
    11821 aacgacaaaa ccatattaat ggaattgaga acttttggaa ccgggcaaaa cgtcatttac
    11881 gcaagtttga cggcattccc aaagcgcatt ttgagctgta tttaaagggg tacgaacggc
    11941 gttttaacaa cagtgagata aaagttcaaa tttccatttt aaaacaatta gtaaaatcga
    12001 gttta
//
LOCUS       DAWFBO010000051        11970 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFBO010000051 DAWFBO010000000
VERSION     DAWFBO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.051"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            188..622
                     /locus_tag="WGO87_000990"
     CDS             188..622
                     /locus_tag="WGO87_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0392363.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(671..2014)
                     /locus_tag="WGO87_000991"
     CDS             complement(671..2014)
                     /locus_tag="WGO87_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0392364.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(2019..2672)
                     /locus_tag="WGO87_000992"
     CDS             complement(2019..2672)
                     /locus_tag="WGO87_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0392365.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2823..3113
                     /gene="gatC"
                     /locus_tag="WGO87_000993"
     CDS             2823..3113
                     /gene="gatC"
                     /locus_tag="WGO87_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0392366.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3176..4621
                     /gene="gatA"
                     /locus_tag="WGO87_000994"
     CDS             3176..4621
                     /gene="gatA"
                     /locus_tag="WGO87_000994"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0392367.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4618..5550
                     /locus_tag="WGO87_000995"
     CDS             4618..5550
                     /locus_tag="WGO87_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ0392368.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5593..7023
                     /gene="gatB"
                     /locus_tag="WGO87_000996"
     CDS             5593..7023
                     /gene="gatB"
                     /locus_tag="WGO87_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0392369.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGEIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7115..8125
                     /locus_tag="WGO87_000997"
     CDS             7115..8125
                     /locus_tag="WGO87_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0392370.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSSRVVHFYWGARHQDDLYALEEAQGLADRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8255..8887)
                     /gene="pdxH"
                     /locus_tag="WGO87_000998"
     CDS             complement(8255..8887)
                     /gene="pdxH"
                     /locus_tag="WGO87_000998"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0392371.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9109..10158
                     /locus_tag="WGO87_000999"
     CDS             9109..10158
                     /locus_tag="WGO87_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0392372.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKGETRKTATQAY
                     GQKASDGIKPQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(10227..11768)
                     /locus_tag="WGO87_001000"
     CDS             complement(10227..11768)
                     /locus_tag="WGO87_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0392373.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKSGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
BASE COUNT         3058 a         3653 c         2972 g         2287 t
ORIGIN      
        1 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatattttt gggaatctgt
       61 tttaccccaa tatataaagc accatattaa ggcggagttt cttccccact ttgaaccgaa
      121 cccggaaaag acaccgccca agccaatcct gatgctgccc cggcagccaa ccatcaagga
      181 aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt tcctgcccgt
      241 cccgcccgcc gaagccgccg tctttacttg gaaggacggc ggcggcaaca gctattcgga
      301 tgtgccgaaa cagcttcatc ccgaccagag ccaaatcctc aacctgcgga cgcgccaaac
      361 caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg gcgcggcaca
      421 ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa agaaaagaat
      481 tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa tgaacctgaa
      541 agcggtggga aattcaaatg caaaaaacaa ggatgatttg attcggaaat acaataacgc
      601 cgtaaacaaa tactgccgtt aatcggctct agcgcaaacc ccatgccgtc tgaaacggca
      661 cggggtttgt ttatttctgc cagtaggttt tgacgttgac gaactcgtac agcccgaatt
      721 cggacaattc gcgcccgtaa ccggaatctt tgactccgcc gaaaggcagg cgcaaatcgc
      781 tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca aaccgccaag
      841 cgcgttcggt atcggcggta taaatgcagg cacctagccc gaacggggaa tcattggcaa
      901 gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc ccgaatactt
      961 cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg ggataaaacc
     1021 agcctcgccc ttgtgggatt tttccgccgg tcaggcacac cgcgccgttt gaaacggcat
     1081 cttcaacctg cccgtgaacc cggtcccgca aatcttcgcg gtgcagcggt gcaagcgtgg
     1141 tatcgggatg tttcgggtcg cccattttca atttggcgca ttcggcgaga aacagctgaa
     1201 taaagcggtc ggctgcggct tcggttacga tgatgcgctt ggcggcgtta cacgattgcc
     1261 ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc gcgttccaaa tcggcatcgg
     1321 gcatcacgat aaaggcgttg ctgccgccga gctccaacac ggttttcttg aggtttgcgc
     1381 ccgcatgtgc cgcaagaatg cgccccgtat gcgtcgaacc ggtaaacgcc atcgcatcgg
     1441 tatcttcgac tgccttgagc gtgtccgcct catccagcca cgcgcctgcc agaggaatac
     1501 cgtctgaagc caaatcgaac aacgcccgac tgacgcgtgc cacgctgggc gcgggcttga
     1561 cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc tgccagacgg
     1621 gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcgaagcgc acctgactca
     1681 aactcgcctg cgtcgcgatg gttttgtggg caagcagttc gggggcaagg cgggcgtaat
     1741 agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcaag cagcgtccga
     1801 cttcctcaca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg tcggcaaatt
     1861 tttgcaggcg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg ccgcgcattt
     1921 tcaaatccgc cagccgccgt tcaaactccg cataatcttg agcggggcgg cggtaaagcg
     1981 tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag ttgcgggaat
     2041 atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat catacccata
     2101 atgcggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt cccggcaacg
     2161 attaaaatgg cataacaaat cgcactgacc accaaaccgg ccgcgataat caacgcgatg
     2221 tcgccgtatg ttttagccgc cgaagcgtaa ataatcacgg tcgaaatacc gcccgggccg
     2281 atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg ggcggggcgc
     2341 gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc gttgccgttc
     2401 atcatcgaaa tggcgatcag cagcaccaaa atcccgccgc cgacctgaaa cgaaccgacg
     2461 ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc aatcacggca
     2521 aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct gtgcccgttg
     2581 gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa aaaagccaca
     2641 atcagcttgc cgatttccat acccaatccc attatttccc cctccttcaa acccgtgcgg
     2701 caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa ttaaaaattc
     2761 aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca aggatgcttt
     2821 tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga ctgcacctga
     2881 ctgcggaaga aaaagaaaaa tcgcttcaag aattaaacga cattttcact atggtcgaac
     2941 agatgcaaac cattaacaca gacggcatcg aaccgatggc gcacccgcac gaggccgccc
     3001 tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat caggcgggtg
     3061 ctccggaagt acgcaaccgt ctgtacatcg taccgcaagt tatcgaagaa taatccgaat
     3121 atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa gatttatgac
     3181 ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt ccgccgtcga
     3241 actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca acggctatat
     3301 caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac gtatcgcgca
     3361 gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt tctgccaaac
     3421 cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc cctacaccgc
     3481 caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca ccaatatgga
     3541 tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggtgcagcca aaaacccatg
     3601 gaatcttgag cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg ttgccgcgcg
     3661 cctcgcccct gccgcgctcg gttcggacac cggcggctct atccgccaac ccgcatcgca
     3721 ctgcggcatt accggcatca aacccacata cggcacggtt tcccgcttcg gtatggtcgc
     3781 ctacgcctcc agcttcgatc aaaccggccc gatggcgcaa actgccgaag actgcgcgat
     3841 tctgttaaac gcgatggcag gtttcgaccc caaagactcc accagcctcg agcgcgaaaa
     3901 agaagactac acccgcgatt tgaaccaacc gctcaaaggt ttgaaaatcg gcctgcccaa
     3961 agaatatttc ggcgaaggca acagcgccga tgttctgacg gcattgcaaa acaccattga
     4021 tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa ccaagctgtc
     4081 catccccgcc tactacgtcc tcgcctccgc agaagccagc accaaccttt cacgttacga
     4141 cggcgtacgt tacggacacc gtgccgccca attcgccgat ttggaagaaa tgtacggcaa
     4201 aacccgcgcc gaaggtttcg gcagcgaagt caaacgccgc atcatgatcg gcacttatgt
     4261 actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc gccgcctcgt
     4321 tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc cgaccgcacc
     4381 cactgcagcc ccaaaaatcg gagcggatgc ttcgccggtt gaaacctact tgagcgatat
     4441 ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg caggcttcag
     4501 cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg aagccaaaat
     4561 cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac gacccgaatg
     4621 aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta tgcgccttta
     4681 tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa agaaatttac
     4741 gccttcgagc agtttgtcaa cgcctcccct atccgtcagg ggctgttcct ccactgcccg
     4801 caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg caaacgccgt
     4861 ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac agacatcctg
     4921 caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga cggcatcagc
     4981 ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt atctttgcgc
     5041 gacgaagcag gcaaccggct gtatatggcg actttcgcct ttgtcggcac acacctgctg
     5101 acagcctccg tacaagggcc tgcgggtgaa gaagccaaag acactgtccg ccgcataacc
     5161 aaacaacttc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca atatttcgcc
     5221 gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt caaactgcgc
     5281 tggaaactta aaaagcgtgt caaaatgaat tacgacgcat tctggcagga atacggcgca
     5341 agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa agaccttgcc
     5401 gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct ggacaacatg
     5461 gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga cggcatccaa
     5521 acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga tattttagag
     5581 aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt ccaattgaac
     5641 accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc caacgcgcac
     5701 gccggcgtgg tggaatgtgc tttgccgggc gtactgccgg taatgaaccg tgaagtcgtt
     5761 gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa aaacgtgttc
     5821 gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag ccagttggac
     5881 ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt gaaaaccatc
     5941 aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtacatga aggcttgaac
     6001 ggcgcgaccg gtatcgacct gaaccgcgcc ggcacgcctt tattggaagt ggtgtccgaa
     6061 cccgaaatgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca cagcttggta
     6121 acctggctga acatttgcga cggcaatatg gcggaaggtt cgttccgcgt cgatgccaac
     6181 gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat taaaaacctc
     6241 aattccttcc gtttcttgga gcaggcgatt aattacgaag cagaagcgca aatcgagatt
     6301 ttggaagacg gcggcaaagt acagcaggca acgatgctgt ttgatcccga aaaaggcgaa
     6361 acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc cgaccccgat
     6421 ttgctgcccg tcatcatttc agacgctcaa atgcaaaaag ccaaagcaga aatgcccgag
     6481 ctgccgaaag aaatggcagc gcgtttcgtg gcggactacg gcgtgtccga atacgacgcg
     6541 cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc caaagagagc
     6601 ggacaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc actgaacaaa
     6661 gaaggcatgg aacttgccga cagcccgatt accgctccgc gcctcgccgc gctggtaggc
     6721 aaaatcgccg acggcacatt aagcagcaaa ttagcgaaaa aagcctttga agccatgtgg
     6781 gcagaacccg aagccaccat tgccgaaatc atcgaaaaac acggtttgca acagatgacc
     6841 gacaccggcg agattgaagc catggtggac gaagtgctgg caaacaacgc caaagccgtg
     6901 gaacagttta aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca agtgatgaag
     6961 gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc caaactggcc
     7021 tgataagcaa gatgccgtct gaaacccaga tccgacgttc agacggcatt ttcacagtct
     7081 gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta ccctgcccga
     7141 ccaaaccacc tttgccgccg gcgacggtga aaccgttttg gccgctgccg cccgtcaaaa
     7201 cctcaatctg ccccattcct gcaaaagcgg tgtctgcgga caatgcaaag ccgaactggt
     7261 cagcggcgat attcaaatgg gcggacactc cgaacaggct ttatccgaag cagaaaaagc
     7321 gcaaggcaag attttgatgt gccgcaccac cgcgcaaagc gatatcagca tcaacatccc
     7381 cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg aaagtatggt
     7441 tttcaaacac gatgtcgccc tcctgaaact tgccctgccc aaagccccgc cgtttgcctt
     7501 ctacgccggg caatacattg atttactgct gccgggcaac gtcagccgca gctactccat
     7561 cgccaattcg cccgaccaag aaggcatttt ggaactgcac atccgcagac gtgaaaacgg
     7621 tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag gcattgtccg
     7681 cgttaaaggc ccgctcggtt cgtttacctt gcaggaagac agcggcaagc ccgtcatcct
     7741 gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc ttatccgcca
     7801 aggcagcagc cgcgtcgtcc atttctactg gggcgcgcgt catcaagatg atttgtatgc
     7861 cctcgaagaa gcacaagggt tggcagaccg tctgaaaaac gcctgcttca cccccgtatt
     7921 gtcccgcccc agagagtgct ggcaaggaag aaaaggccac gtacaagaca tcgcggcaca
     7981 agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg ctatgaccga
     8041 acaggcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt ttttctccga
     8101 cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg ctttccgttc
     8161 ggaacacaaa aaacttcccg tccgtatttt ccccgtgaaa aaatgccgtc tgaaacccga
     8221 ttcctgtttt cagacggcat atgttttttc ctgttcaagg tgacagccgc tcgcgtatcc
     8281 agccgccatc cagcaaacgg tattggatgc ggtcgtgcag cctgctcggc ctgccctgcc
     8341 aaaactcaat caaatcggga atcacaatat agccgcccca atgcggcgga cgcggcacgt
     8401 gcaaaggatg tttgagtccg accgccgccg cctttgccac caataccgcc ttgttcggaa
     8461 tcacttcgct ctgcgcgctt gcccacgcac ccaaacggct ctgatacggg cggctctcaa
     8521 aatattcgtc cgacaatttc tccgccagcc tttcaacacg cccttccacg cgcacctgac
     8581 gctccagctc cggccaaaaa aacgtcatcg ccgcaaatgg atgagcatcc agcgaacgcc
     8641 ccttgcggct gtgataattc gtaaagaaaa caaaaccttc agaattaact tccttcagca
     8701 gcaccatacg gctgttgggt ctgccgcgtc cgtcgaccgc cgccacatta accgccgtcg
     8761 gctcgttaac ctgtgcgcgt accgcctcgt ccaaccaccg ctcgaactgc tcgatcggat
     8821 tgtcggcgca atcggcttcc gacaattccc gtttgctgta atcttcccga atattgtgca
     8881 aatccatgtt accgctcctc ttgttatttg aaagattcta cccccgcacg caaaccgatt
     8941 tcaaccgttg cacaaacttt gtccccaaag ccgcagcgac gatttcatcc gcaaaaccgc
     9001 cgcatcaggt acaatatcga accgtccgac tgaggacggc attttatcaa cccgtcctgc
     9061 cgcatacgcc gcagaagaac cgctttatca agcgagttag gaaaaatgat gtccaaacag
     9121 cccaccagca aacgccaatg gcgcgacggc gcagccccgt ctgccaagaa aaccgccaaa
     9181 cctttcaaaa gcaaagcccg tcccaaaggc gaaacgcgca aaaccgccac ccaagcttac
     9241 ggacaaaaag cttcagacgg catcaagccg caaaacgccc ccaaacagcg cgccgccaaa
     9301 gccaaaaaac tcgtcgtccg caatcccaac caaaaaatta tggaacacgc gcgcgatttg
     9361 aaagaacgcc gcagcgacct gtcgcgcatg gaacccgaac gcctgcaaaa agtgcttgcc
     9421 gcgtccggcg tcggctcgcg ccgcgaaatg gaagaatgga ttaccaacgg ctggataacg
     9481 gtcaacggca aaaccgcgca actgggcgac aaagttaccc ccgacgacca cgttaccgtc
     9541 aaaggcagca tcatcaagct caaatgggcg gaccgcctgc cgcgcatcat cctgtattac
     9601 aaacaagaag gcgaaatcgt ttcccgtgac gacccgcaag gccgcgtcag catattcgac
     9661 cgcctgccgc aggccgccag cagccgctgg gtcgccatcg gacgcttgga catcaacacc
     9721 agcggacttc tgattcttac cacctccggc gaactcgtcc aacgtttcgc ccaccccagc
     9781 ttcgaagtcg aacgcgaata cgccgtgcgc gtcttgggcg ggctgaccgg cgaacaaatg
     9841 cgcgtcctca ccgaagaagg cgtgatgctc gaagacggct tggcaaaagt cgaacgcatc
     9901 tacgaacaag gcggcgaagg cgcgaacaaa tggtacaacg tcgtaattaa agaaggccgc
     9961 aaccgcgaag tgcgccgcat attcgaaagc caaggcctca ccgtcagccg cctcgtgcgc
    10021 gtcggcttcg gtcccatcgg actgcccaac cgcctcaaac gcgggcagtt ctacgaactc
    10081 aatcccgccg aagtcgccaa catcatcaaa tgggcggaca tgctgctgcc gggcgaacgc
    10141 cgccgcaaaa aagcctaaac ccgccaaaac acaaaaatgc cgtctgaaac atctactgtt
    10201 tcagacggca ttttgttcgg gcgttttcag gagaaaaggt cgagtgcttt gacaaagatc
    10261 atcaccacgc cgtaggcgag cggtatgacg gccaatgccc aacgccacca gaccgatatg
    10321 ccgccgccgg aaactttttc gcccacaagg taagcgtcgg atatggcggt ttcgtcatcg
    10381 gggttgccgc tgtgtgcggc ggttttgatg tctttttcgt ggtgtttttc gtgtacggat
    10441 ttgacggcga ggttgcacaa caaaccgata atcagcagac ccgccatgat gtacatggtt
    10501 acgctgtatg cctgtgccgc cggtatgccg ctgtcgattt ggctttggcg tatgtaattg
    10561 accagtaccg ggccgatgac ggcggcggtt gaccaggcca gcaggatgcg cccgtgaatc
    10621 gcgccgacct gataggtgcc gaacaggtct ttcaggtagg cgggaatggc ggcaaatccg
    10681 ccgccgtaca tggaaataat cacgcaaaag ccgatgatga acagggcttt gctaccaccc
    10741 tcgccgatgg agggaacggc gaaatacagc agcgaaccga gtacgaagaa aatggtgtag
    10801 gtgtttttgc gtccgatttt gtcggaaacg ctcgaccata aaaagcgtcc gcccatgtta
    10861 aacaggctaa ggaggctgac gaagcctgcc gccgcacctg caccgactgc cgcctgcctg
    10921 cctacggagg tttcggaaaa gagttcctga atcatcacgg atgcctgacc caatacgccg
    10981 atgccggcag ttacgttcag gcacaatacc cagaacaaca gccaaaactg cggcgttttc
    11041 atggcttggg acacgttgac atgattgctg ctgaccagct tgttttgcgt tttcggcgcg
    11101 gtatagcctt caggtttcca gccgtcggca ggtacgcgga tggtaaacgc gccaaacatc
    11161 atcagtgcga ggtaaagcag acccaatacg gcgaaggttt cggcaacccc aaccgaagca
    11221 gcgtttgaaa aggcgttcat cagtgatacg gaaagcggcg aggccagcat tgcgccgccg
    11281 ccgaaaccca taatcgccaa accggtcgcc atacccgact tgtcgggaaa ccatttcatc
    11341 agtgtggaaa ccggcccgat gtagcccaaa cccaagccta cgccgccgat gacgccgttg
    11401 cccaaataga gcaggaagag gttgtgcgta cgcacgccga atgcggatac gaagaagccc
    11461 aggctgaagc agcaggcggc ggcaaatatg gctttgcgcg gccctacccg ttccatccac
    11521 gtaccgaaca gggcggccga cgcgcccagc atcgcgagtg cgatactgaa aatccaacct
    11581 acggtcgtca gcttccaatc tccggccgcc gattcggtta tgccgataag tttggtcagc
    11641 ggcgcgttga atacggaata ggcgtaaatc tgcccgatgg caaggtgcac cgccaatgct
    11701 gcgggcggta cgagccaacg gttgaaaccc ggcttggcaa tgcttgcctc acggtctaaa
    11761 aacttcataa catcctcttt ctgtcagttg aaaaataaaa tttcatttgc tcaatggaaa
    11821 cttattgaaa actataaaaa aatatcggat cggtttttta tccgccccaa gatgccccgt
    11881 ctgaaacatt tcgggtgtac ggaaaggttt ctgttttttc cgacaaattc ctgcggcttt
    11941 tcgcttccgg attcccgctt ttgcgggaat
//
LOCUS       DAWFBO010000052        11743 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFBO010000052 DAWFBO010000000
VERSION     DAWFBO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.052"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            46..261
                     /locus_tag="WGO87_001001"
     CDS             46..261
                     /locus_tag="WGO87_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392374.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANAVAMICIVWIAVL"
     gene            complement(259..374)
                     /locus_tag="WGO87_001002"
                     /pseudo
     CDS             complement(259..374)
                     /locus_tag="WGO87_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            590..1891
                     /gene="bioA"
                     /locus_tag="WGO87_001003"
     CDS             590..1891
                     /gene="bioA"
                     /locus_tag="WGO87_001003"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0392375.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLHDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMAHFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGLLTELADKTVHILKEHNK"
     gene            1888..2535
                     /gene="bioD"
                     /locus_tag="WGO87_001004"
     CDS             1888..2535
                     /gene="bioD"
                     /locus_tag="WGO87_001004"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0392376.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKLVQTGC
                     QNIADDIAVHRKIMGIPMQEADKQGLTMPEIFSYPASPHLAARLDGRDLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2502..2732
                     /locus_tag="WGO87_001005"
     CDS             2502..2732
                     /locus_tag="WGO87_001005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392377.1"
                     /translation="MDGVGKNRRGIKIGKNMEHLYDEYGNTTPNRRTTNKKDETISSK
                     VTVAAIPVAAPFAIADIVDQDFIQAIMKITGH"
     gene            2756..3142
                     /locus_tag="WGO87_001006"
     CDS             2756..3142
                     /locus_tag="WGO87_001006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392378.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            3136..3579
                     /locus_tag="WGO87_001007"
     CDS             3136..3579
                     /locus_tag="WGO87_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392379.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            4037..4513
                     /locus_tag="WGO87_001008"
     CDS             4037..4513
                     /locus_tag="WGO87_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0392380.1"
                     /translation="MGYGFEEWLPDLPAPVSDGISLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            4543..5433
                     /gene="ubiA"
                     /locus_tag="WGO87_001009"
     CDS             4543..5433
                     /gene="ubiA"
                     /locus_tag="WGO87_001009"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0392381.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVLPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGATWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            5618..6067
                     /gene="ptsN"
                     /locus_tag="WGO87_001010"
     CDS             5618..6067
                     /gene="ptsN"
                     /locus_tag="WGO87_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0392382.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            6069..7031
                     /locus_tag="WGO87_001011"
     CDS             6069..7031
                     /locus_tag="WGO87_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0392383.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            7012..7866
                     /gene="rapZ"
                     /locus_tag="WGO87_001012"
     CDS             7012..7866
                     /gene="rapZ"
                     /locus_tag="WGO87_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0392384.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIAYLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPKLRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            7947..8888
                     /gene="ylqF"
                     /locus_tag="WGO87_001013"
     CDS             7947..8888
                     /gene="ylqF"
                     /locus_tag="WGO87_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0392385.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            8989..9591
                     /locus_tag="WGO87_001014"
     CDS             8989..9591
                     /locus_tag="WGO87_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0392386.1"
                     /translation="MTDKISPDALIEAALLTQTEPLNEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVIQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPTTVPSDTLPN"
     gene            9775..11448
                     /gene="recN"
                     /locus_tag="WGO87_001015"
     CDS             9775..11448
                     /gene="recN"
                     /locus_tag="WGO87_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0392387.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPTLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            11558..11634
                     /locus_tag="WGO87_001016"
     tRNA            11558..11634
                     /locus_tag="WGO87_001016"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11592..11594,aa:Arg,seq:acg)
     gene            11652..11726
                     /locus_tag="WGO87_001017"
     tRNA            11652..11726
                     /locus_tag="WGO87_001017"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11685..11687,aa:Glu,seq:ttc)
BASE COUNT         3049 a         2778 c         3041 g         2875 t
ORIGIN      
        1 ggctgtaacc ttcaatcgga atttgttgcc tgcgggatat gcggtatgaa tgtttgggat
       61 atatggggca ggatggtgga aatctatcat aagtataaga agccgtgcct ggttttggcg
      121 gtggattttg tgatgggtat ggtattcata gagccgaatg aggagccgtg catcggcaga
      181 tgctatgcgc caatgtcgga gtcccctgat tttgccaacg ctgttgctat gatctgtatc
      241 gtatggattg ccgttttata gtggattaaa tttaaaccag tacggcgtta cctcgccttg
      301 ccgtactatt tgtactgtct gcggcttcgt ctccttgtcc tgatttttgt taatccacta
      361 tatctatgac tgattgaagc gttgggcgga ggctgtgtga aacggtattg ggcgttgggt
      421 cgtctgattc caatcaggct tgaggaatgc gaaacggtgt gcgcttatgc tgaggacgat
      481 ttgtttcgcg gttttgcgcc cgaaacggat ggaaaggtgt gggaagcggt ctgtcggagt
      541 agaatacgcg tttgcgtttg aatacagtaa gaagaaaaga gagaaactta tgccgtctga
      601 acatcaacac acatcatcat tgcttgattt cgaccgtacc catctgcttc atccctatac
      661 ttccatgacc gatccgctgc ccgtttatcc tgtcaaacgt gcagaagggg tgtttatcga
      721 attggcggac ggcacgcggt tgattgacgg gatgtcgtct tggtggtgtg cgatacacgg
      781 ctacaatcat cctgttttga atcaagcggt tgagacgcag atgaaacaga tgtcgcatgt
      841 gatgttcggc ggtttgacgc acgaaccggc agtggagctg ggcaaattat tggttgggat
      901 tttgccgcag ggactggacc gtatttttta tgcggattcg ggttcggttt cggtagaagt
      961 cgcgctgaag atggcggtgc aataccagca ggcgcggggt ttgaccgcga agcagaatat
     1021 tgcgacggta cggcgcgggt atcacggcga tacttggaac gcgatgtccg tctgcgatcc
     1081 ggaaacgggg atgcaccata ttttcggcag cgcgttgccg cagcgttatt ttgtcgataa
     1141 tccgaaaagc cgtttcgacg atgaatggga cggggcggat ttgcagcctg tccgcgcctt
     1201 gtttgaagtg catcatgcgg atattgccgc ctttatttta gagccggtcg tgcagggcgc
     1261 gggcggcatg tatttttatc atccgcagta tcttcgcgga ttgcacgatt tgtgcgacga
     1321 gtttgatatc gttttgattt ttgatgaaat cgccaccgga ttcgggcgca cgggcaagat
     1381 gtttgcctgc gaacacgcgg aggtcgtgcc ggatattatg tgcatcggca agggtttgag
     1441 cggcggctat atgacgctgg cggcggcaat cacttcgcaa aaagttaccg aaacgatttc
     1501 gcgcggcgag gcgggcgtgt ttatgcacgg accgacattt atggcaaacc cactggcgtg
     1561 tgccgttgcc tgcgcttcgg tcaaactgct tttgtctcaa gactggcagg caaatatccg
     1621 ccgcattgaa agcatcttaa aaggccgtct gaaagccgcg tgggacattc gcggcgtgaa
     1681 agacgtacgc gttttgggtg ccatcggcgt gatcgagctg gataaaggcg tagatatggc
     1741 gcattttcaa gcggattgcg tggcgcaggg tatttgggtg cgccccttcg gcaggctggt
     1801 gtacctcatg ccgccttaca ttatttcaga cggcctcttg accgaacttg ccgataaaac
     1861 tgtacacatt ttgaaggaac acaacaaatg aaaggcgttt acttcgtcag cggcattgat
     1921 acggacatcg gcaaaaccgt cgccaccggc gtattggcaa aacaattgtt gcagcaaggc
     1981 aaaagtgtga ttacgcaaaa gctcgtgcaa accggttgcc aaaatatcgc cgacgacatc
     2041 gccgtccacc gcaaaatcat gggcataccc atgcaggaag ccgacaaaca agggttgacc
     2101 atgcccgaaa tcttcagcta tcccgcctcg ccccaccttg ccgcccgtct ggatggcagg
     2161 gatttggact tggacaaaat ccgtaccgcc acacaagaat tggcggcgca gtacgaagtc
     2221 gttttggtcg aaggcgcggg cggattgatg gttccgctga cggaaaacct gttaaccatt
     2281 gattatatcc gtcagcaagg ctatcccgtc atcctcgtta ccagcggacg gctcggcagc
     2341 atcaaccaca ccctgctcag tttcgccgcg ctcaaacaat acggcattcg cttgcacagc
     2401 ctcgtgttca accacatcca cgacagccgc gacgcacaca tcgcccaaga cagcctgagc
     2461 tacttgaaat gccgtctgaa agccgatttt tccgaagcgg aatggatgga gttggcaaaa
     2521 acagacgcgg tataaagatt gggaaaaata tggaacacct atatgatgaa tatggaaata
     2581 caacgccaaa tagaagaaca acaaataaaa aagatgaaac tatttcaagt aaagtaactg
     2641 ttgcagcgat accagtagcc gctccatttg ctattgctga tatagtcgat caggatttta
     2701 tccaagcgat tatgaaaatt actggtcatt gattatctat atattggtgg ttgttatgaa
     2761 atgctatatt aaagttgtca ttattgtttt tataatggta atgacaactt cttgtaatcc
     2821 ttgctttttt gcaacttctt cttgtgatta ttcttttaga aaaaactatc ctataaatag
     2881 cgacgatgaa tattatgtta agaataatac gagcaaagaa acaagaattg cggatttacg
     2941 ctattgtatt tcttatgcat gtccagaagt ttatcaaaaa tatcaactac cagatgaaaa
     3001 tggagattat agggagatta ttcgtagaaa atataaagat gattataaaa agggctataa
     3061 gaatgcagat gatgtcatga catgctacgg aaagattgga gaatgcatga aaaataataa
     3121 aggatattca acaaaatggt aatgttccgc ttattatcaa cagcccttgt attgttgata
     3181 atgcagggct gttattacac aactattgat aatgaaaaat gctttgttgc cccacaaagc
     3241 gttcattgtc ctgtaagttc tgatattgaa aatgttatta gaaaaaatcc taataaaagc
     3301 ataaatcaag ttaggagtga tattaaatat tgtgctaata tgtatttttc caatatacgg
     3361 gaattaaata ttccgcaaga ttttgataat tacattttca tgcttaaaga aaaaggtatt
     3421 ccaagaaatg ataaggaatt ggcttataat gcagttagaa atatgcacat tatttgtttg
     3481 agttctttgg gtggttatga ttattatcaa aaaaccgcag aatttcagaa aatagattat
     3541 aaaaaagaaa tggagaaatt gttaaatgaa aaccattaaa tttgctattg ccgatatagt
     3601 tgatagcgac tttatccaag tgattatgaa attaagcggg cattgattta tgattacgcg
     3661 ttgttttaaa gttgtcatta attgttttta taatggtaat gacaacttct tgtaatcctt
     3721 gattttttgc aacttcgtct tgtaattatc cttttgatga tatgacgata agagcaaaca
     3781 acgatattga atattttgtt aaaaataata cgactaacga acaacgaatt gatgatttca
     3841 gaagttgcct ttccgttgct tgtcctgaaa tatataaatt atatcaatta cctgatgaaa
     3901 agggtaatta tcaatatatt ataaaaaaat attatgataa taaatacaat gataatgcag
     3961 tcaaatcgac aaattgtatt ggggataatg caagatgtat gcttaataat aaaggatatt
     4021 caacaaaatg gtaataatgg gttacggctt tgaggaatgg ctgcccgacc tgcccgcccc
     4081 tgtttcagac ggcatcagcc tgccgatgag ccggttattg aaagcccggt cgctgaccgc
     4141 cgcattgcgc gccttgccgc atacattttc ggtggaactg ctgaaactgg gcgaattgga
     4201 gacggaatgc ggagggaggc tggtgcgcga agttttgttg aagctggacg gtatccctgt
     4261 tgtcgcggca aggagcgagt gccggatagg ttcggcgttt tggcaaaaca ttttggactg
     4321 cggcacgcgt cctttgggcg agcgtctgtt tcaagccgat ttggaagggg cgcgttcggc
     4381 gtttgagttt gccgtttccg gcgaaggatg cggacggtac tttgccgcgc ggcgttctcg
     4441 gttttcccgt cacggcgagg aaatgctgct gaccgagtat tttctgcccg aactgaaacg
     4501 ctttatcgga taaaataccg ttttttcaag ctgtgcggca atatgaatcc taaatcccct
     4561 ttatttttac gactgtccga tcgtttggat gtgtacctgc gcctgatgcg ggcggacaag
     4621 cccattggga cgctgctttt gctgtggccg acctactggg cattgtggct ggcttcagac
     4681 ggcattcccg atttggcggt attggcggcg tttacaatcg gcacgttttt aatgcggagt
     4741 gccggttgcg tcatcaacga ttttgccgac cgcgattttg acggtgctgt cgagcgcacc
     4801 aaaaaccgtc cgttcgcaca gggtcgggtc ggaaaaaaag aagcgctgct gctgacggca
     4861 tttttgtgcc tgcttgccgc attgtgcctg attccgctga atcatctgac atggctgatg
     4921 agcctgcccg cgctgtttct tgcgctgact tacccgttta ccaaacgctt ttttccgatt
     4981 ccccaactct atctcgggct tgccttttcc ttcggtatcc cgatggcgtt tgccgccgtt
     5041 gccggaaacg tgctgcctca agcgtggata ctctttgccg ccaatgtgtt atggactctg
     5101 gcgtatgaca cggtttatgc aatggcggac aaagaagacg atttgaaaat cggcatcaaa
     5161 acctccgccg tcacgttcgg gcgttacgac atcgccgccg ttatgctgtg tcacggaggc
     5221 tttaccctgc tgatggcagt attgggtgcg gttatcggtg cgacatgggc atattggacg
     5281 gcaatcccca tcgtcctgct gctgcaatac cgccaatatg ccgccatcaa aagccgcgtc
     5341 cggcaaatct gttttgaaac gtttttggca aacaacagaa ttggttgggt gtggtttacc
     5401 gccatttttg cccatacgtt tttcgcgaaa taaggcaggg caatgccgtc tgaagagccg
     5461 taaactgctt tggacggcat ttctatctgt gccgaaaagc gttaaaatat gtttttaaaa
     5521 cgctgtgtta tgtcagcccg tgccgtatgc gggattgaga tttgccccgg cagtcggtac
     5581 aatctttctg ttttgcgatg tctgaaaaga gaagcttatg agccttatcg gcgaaatttt
     5641 gcctttgtcc catattgttt tggatatgga ggtgggcagt aaaaaaaggc tgtttgagga
     5701 agcgggcctg cttttggaac gcgaatcctc attgtcccat gctgatgttt tcgaatgtct
     5761 ttttgcccgt gaaaaactcg gttcgaccgg tttggggcag ggcgttgcca tcccgcacgg
     5821 ccgtcatgcc ggcgtgaagc aggcgacggg cgcgttcatc cgcacgcgcg aacccgtcgg
     5881 atttgacgca ccggacggca aaccggtttc cctggttttt atcttgctgg ttccggaaaa
     5941 tgcgacggga gagcatttgg aagtattgtc caaactggcc ggcaagtttt cccaaaaaag
     6001 catcagagaa tcgctgatga ccgtttcttc tgcggaagaa gcccgcgcca tcctgactga
     6061 agaataatat gcccagtatc tctgtccgcc ggctgtttga cgacaaccaa tacaaactgc
     6121 aacttgcctg ggccgccggc aattcgggtg cggacaaccg tatcggcgta gaggcggaca
     6181 agcccgtcct cgccttggtc ggacacctga atttcatcca ccccaaccaa atccaagtgg
     6241 tcggtttggc ggagtcggaa tatctgaacc gtctcgaatc gggggaaacg ggttatcagt
     6301 tcggagacct gttcgacatt tctatgtctt tggtcattgt ggcaaacggc ttgccggttt
     6361 ccccgggtct gcgcgactat tgccataaaa acgatattcc actgctgact tccaaactcg
     6421 aaagccccta tctgatggat gtgttgcgga tttacctgca acgcaccctg gcggcatcgt
     6481 ccgtcaaaca cggcgtattt ctcgatgtgt ttgaaatcgg cgtgctgatt accgggcatt
     6541 ccggcctggg taagagcgaa ttggcattgg aactgatttc gcgcggccac agcctgattg
     6601 ccgacgatgc ggtcgagctg ttccgcatcg gcccggaaac gctggaaggg cgttgttcgc
     6661 ctatgctgcg cgattttttg gaagtgcgcg gcttggggat actcaatatc cgccatattt
     6721 tcggcgaaac ttccatccgc cccaaaaaaa tcctgcaact cattatcaat ttagtcgagg
     6781 cggacgacga gtatatgaag cagctcgacc gtttgagtat ccgtaccgaa accgaatcca
     6841 tcctcaacgt caacgtccgt tcggtaacgc tgcctgtcgc ggtcggacgc aaccttgccg
     6901 tattggttga agcggcggtg cgcaattata ttcttcagtt gcgcggtaag gacagtacgc
     6961 gcgaattttt ggaacgccat cagacacaac ttaaagaaaa cgaacagaat catgaaaatc
     7021 gtcctgatta gcgggttgtc cggttcgggc aaatccgtcg cactgcgcca aatggaagat
     7081 tcgggttatt tctgcgtgga caatttgcct ttggaaatgt tgcccgcgct ggtgtcgtat
     7141 catatcgagc gtgcggacga aactgaattg gcggtcagcg tcgatgtgcg ttccggcatc
     7201 gacatcggac aggcgcggga acagattgcc tatctgcgcg gactggggca cagggttgaa
     7261 gttttgtttg tcgaggcgga agaaagcgtg ttggtccgcc ggttttccga aaccaggcga
     7321 ggccaccctc tgagtaatca ggatatgacc ttgttggaaa gcttaaagaa agaacgggaa
     7381 tggctgttcc cgcttaaaga aatcgcctat tgtatcgaca cttccaagat gaatgcccaa
     7441 cagctccgcc atgcagtccg gcagtggctg aaggtcgaac gtaccgggct gctggtgatt
     7501 ttggagtcct tcgggttcaa atacggcgtg ccgaacaacg ccgattttat gttcgatatg
     7561 cgcagcctgc ccaacccgta ttacgatccc aagttgaggc cttacaccgg tatggacaag
     7621 cccgtttggg attatttgga cggacagccg cttgtgcagg aaatggttga cgacatcgaa
     7681 aggtttgtta cgcattggtt accgcgtttg gaggatgaaa gcaggagcta cgttaccgtc
     7741 gccatcggtt gcacgggagg acagcaccgt tcggtctata ttgtcgaaaa actcgcccga
     7801 aggttgaaag ggcgttatga attgctgata cggcacagac aggcgcaaaa cctgtcagac
     7861 cgctaattcc gtcaaaccat tatgccgtct gaaaccccgt cctccgtttc agacggcatt
     7921 tcaaatattg aaaagaaaga acagacatgg caatccaatg gtttcccggc catatgaaca
     7981 aggcgaaaaa agccatcgcc gagcgtgcaa aaagcgttga tatggtgatt gaaatgctgg
     8041 acgcgcgtat gcccgcctcc agcgaaaacc ccctgcttgc ccagctttcc aaaggcaaac
     8101 ccaaacttaa aatcctaaac aaacaagatc ttgccgaccc cgaacgcaca aaggtctggc
     8161 ttgaccacta caacagccgc cccgacacgc gcgccatcgc cctcgattcc tccgaaaccg
     8221 gcgcacacgg caaaatcacc caagcctgcc gcgcgatgat tccccaccgg caaggcatag
     8281 acaaacccct gcgcgttttg atttgcggca ttcccaacgt cggcaaatcc accctcatca
     8341 acggcatgat aggcaaaaaa tccgccaaaa ccggcaacga acccggcatc accaaagccg
     8401 aacaacgcct tttccttgcc gacgacttct ggctgtacga cacccccgga atgctgtggc
     8461 cgaaaatcat tgtcgaagaa agcggctaca acctcgccgc cggcggttca gtcggacgca
     8521 acgcattgga cgaagaagaa gtcgccctcg aacttttaga ctacctccgc cgccactacc
     8581 tccctatgtt gcaagaacgc taccaagccg acaaagaccc cagcagccac tgggacgaaa
     8641 acgtttggct cgaatggata gccaaaaaac gcggcgcagt cctcagcggc ggacggatca
     8701 actaccaaaa agccgccgaa aacatcctca ccgacttccg tgaaggcaaa atcggcagaa
     8761 tcaccctcga aacgccgaac caatgggaaa cttggctcaa aaaagcccgt cagaaagaag
     8821 ccgaactcaa agccatacgc gaagccagaa aagcagagcg taaagggcag aagccttcgg
     8881 aagcataaag aatgccgtct gaaaaatatt tttcaggcag cttctctcta ctacaaccga
     8941 tttcagacgg catatccaaa cccatgccgt ttcagcacgg atacccgtat gaccgacaaa
     9001 atttctcccg acgcgctgat tgaagccgca ctgctgaccc aaaccgaacc gctgaacgaa
     9061 aaatctatgc gcgaattgtg tgtgccgccg ttgtcgcaag acaaactgat tgatgtgttg
     9121 gcgcagttga aaacgcgttg gcaggatagg gcgttgcaac tggtgcatac gcaagagggc
     9181 tggcgttttc agattgttca gacggcattc gagcggctgg gcagcctgca agaacagcgt
     9241 gcgccgcgct actcccgcgc cgtgatggaa acactggcga ttatcgccta ccagcagccc
     9301 gtaacgcgcg gcgacatcga aggcatacgc ggtgtggcgg tgtctcagaa cgtgatccag
     9361 accttgcagg atcgggggtg gattgaagtc atcggacatc gggacacatt gggaaaaccc
     9421 gcattgtggg caacaacggc aacgtttctc agcgatttgc gcttggacgg tctagaagaa
     9481 cttcctccgc tgaccgaact gggcgaactg gttttgcccg atttaatgga aatgccgcct
     9541 acggatgaag aagagccgac aaccgtacca tccgataccc tgcccaactg aaattccaaa
     9601 tgccgtctga aacgcacatt gcttcagacg gcattgcaac aaataagcag ataaaaacaa
     9661 gcactaagaa aaattaagga aaaacttatt tttaatttaa aaaatcttag ttataattcg
     9721 tatatctaaa gttgatattg cttttgtcgg tagaattgct aaggaatcct cacgatgctt
     9781 ctaacacttt ctttgcgtga ttttgtcatt gttgaaaatc tgaatctgga ttttcaaagc
     9841 ggctttaccg tattgaccgg agaaactggc gcgggcaagt ccattacttt ggatgcgatt
     9901 ggtctgctgt tgggcgataa agccgattac agccaagtcc gcagcggcgc aaaagaagcg
     9961 cagttgtcgg cattgttcga tatttcccat ttgcctactt taaaagcaga attgtatgaa
    10021 caggggcttt taaacgacgg agaagaagaa ctcagtatcc gccgcattat cgatgccaaa
    10081 ggcaaaagcc gcagctttat caacaatcag gccgctacct tggcgcaact caaagccgtc
    10141 ggcagccagc ttatcgacat ccacgggcaa aacgcccatc attcgcttaa tcaggaagcc
    10201 gcccagcgcg aattgttgga cgcatttgcg ggcagcaggg cgcaggcgga aaccgtcagg
    10261 cagctttatc aaaactgggc aaactcaaaa aaagcattgc aggaagcgca ggaacaggca
    10321 gaaaacattg cactcgaaca agagcgtctg gaatggcagt ttaacgaatt gaatcagttg
    10381 gacattaaac aaggcgaatg ggaagccctc agccaaagcc acgacagcct tgcccattct
    10441 gccgagctgt tgcaggctgc cgaagaagtc ggaagcaaga ttgacggcga caacggcatc
    10501 caacgccata tctatcaatg tcaaaaacta ttggccaatc tgcaaaacat cgagccgcgc
    10561 tttgccgaga gcctgaatat gttggcaagc atcgaggccg aattgggcga aatcagtgcc
    10621 aatatgcgcg atgtggcagg acgcagcgac atcaacccca atgaattggc ggcacaggaa
    10681 cagcgtatgg gcgaactgat ggggatggcg cggaaatacc ggatcgagcc tgaagagttg
    10741 cctgccaagt tggcagaaat cgaagaacgc ctgcaaagcc tgcaagctgc cgccgatttg
    10801 gacgcgctcg agcataatgt tgcccacaat tttgccgaat atcaggaagc tgcccacatc
    10861 ctttctgcca tgcgccatca ggcggcagag cgtttgagcg gcgaaacgac cgagcatatg
    10921 caacaccttg ccatgaaagg tgcacgtttc gacatcgtcc tgttgccctc gtcgccgacg
    10981 gcacacggtt tggagcaggt tcaatttcaa gttgccgcca acaaaggcaa tccgccccgt
    11041 ccgctgaata aagttgcctc cggcggcgaa ttggcgcgta tcagccttgc cttacaggtt
    11101 gttgccagcc aatataccca agttcccacc ctgatttttg atgaggtcga taccggtatt
    11161 ggagggggag tggctgaaat ggtcggcaag gcattacgtg cgttgggcag aaaacatcag
    11221 gtgcttgccg ttacccatct tccgcaagtc gcatcctgcg gcgaaaacca ctggcgggtg
    11281 cgcaagcaca gcgagggaga gcaaaccgtc agcgaaatca gtatattgga tgaaatccaa
    11341 cgggtcgaag aggttgcccg tatgttgggc ggagaagtca ttaccgatac gacgcggcag
    11401 catgcggcag aattgctgca acttgcgtcg aaaaatagtt tattttaaaa tcaatcagtt
    11461 aaaaaataac taaaaataaa agtctaaaac aatagacaga actcagataa atccgtatta
    11521 tcacgctttc ttaatcactt gaacaagtga ttgtgctgca cccgtagctc agttggatag
    11581 agtatctggc tacgaaccag agggtcgggc gttcgaatcg ctccgggtgc gccagtaaga
    11641 aaatacaata tgcgcccatc gtctagcggt taggacatcg ccctttcacg gcggtaaccg
    11701 gggttcgatt ccccgtgggc gtgccaattc aaaatgcctc cga
//
LOCUS       DAWFBO010000053        11701 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFBO010000053 DAWFBO010000000
VERSION     DAWFBO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11701
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.053"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(15..218)
                     /locus_tag="WGO87_001018"
     CDS             complement(15..218)
                     /locus_tag="WGO87_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392388.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(196..429)
                     /locus_tag="WGO87_001019"
     CDS             complement(196..429)
                     /locus_tag="WGO87_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0392389.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            962..4396
                     /gene="dnaE"
                     /locus_tag="WGO87_001020"
     CDS             962..4396
                     /gene="dnaE"
                     /locus_tag="WGO87_001020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0392390.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELSLPVVATHPTQFMSSDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQSNRGRVIEYVREKYGAKAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITSARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMNNADQKAANTNQGGL
                     FDMMEDAIEPVRLVDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLS
                     SHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            4674..7922
                     /gene="cas9"
                     /locus_tag="WGO87_001021"
     CDS             4674..7922
                     /gene="cas9"
                     /locus_tag="WGO87_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ0392391.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            7989..8903
                     /gene="cas1"
                     /locus_tag="WGO87_001022"
     CDS             7989..8903
                     /gene="cas1"
                     /locus_tag="WGO87_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ0392392.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAKHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            8896..9222
                     /gene="cas2"
                     /locus_tag="WGO87_001023"
     CDS             8896..9222
                     /gene="cas2"
                     /locus_tag="WGO87_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ0392393.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   9286..10971
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9286..9321
                     /rpt_unit_seq="attgtagcactgcgaaatgagaaagggagctacaac"
     gene            11411..11701
                     /locus_tag="WGO87_001024"
                     /pseudo
     CDS             11411..11701
                     /locus_tag="WGO87_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3334 a         3072 c         2807 g         2488 t
ORIGIN      
        1 aagccgaacc gttttcagac ggcatcgtcc gaccaacccg cttctttcaa tttccgccgt
       61 tgcgcgtcgt agcgggcctt atccgcccaa taaatcgtct gaatacacgc ctcgccgcag
      121 tcgctgccgc agcattccca cgactcgggt cggacgggtt cgtctaaaag cggctcgccc
      181 aaaagggctt cggctttatg cttcagggtc atatccatcg gcgatttcca agcgcgcacc
      241 gtcaaactcg atgacttcgc cgccgcgtat tttggcggtt ttacgggttt cggtttcgcc
      301 gttgcgcgac accaaccctt cggcaatcaa cgccttcgcc tgtccgccgc tttcggcaag
      361 tccggccaat ttcaagaggt cgcacagggc gatgtattcg ttgtcttcga gatagacggt
      421 ggcttccata atgttccctt gcagaaagag gccgttattg tagcacctgc cgccgccgta
      481 cccaaaatta ccgcaaaaca ggcagcacat cccgccccgc ctgttccgta aaaatgccgt
      541 ctgaaacccc atatgccgtc atccgatcaa agaaatcctg tccaacggca gactgcaaat
      601 cctgttcccc gacgaatccg cattgacgct gatgcacatc ctcaaacgcg aactgcccga
      661 tacaccggca atcggcatca aaacgaaatc aaaaacctgt ttgaacgtaa aattaattta
      721 actgcttgcc atccgtccga ataaggtata tagttcctta taactagttt gatagtcctt
      781 tatatctatc aatactcctt gggaagcctc cgccatacgg caggaggcat ttttttgccg
      841 ccgcaaaagc tcaaaaacat ttgcaggtca tgccgtctga atccgaaaca gcattaccta
      901 caccgccatc taaagacaac cctgctacaa tacgcctttt attgtccacg ccgattttgc
      961 catgaccgag ccgacctaca tccccctgcg cctgcatacc gagttttcga ttaccgacgg
     1021 tatggtgcgg attaaaaaac tgattgccaa agcgcaggaa tacggtttgc ctgctttggg
     1081 catcagcgat ttgatgaacg aattcggctt ggtgaaattc tataaagcct gccgcagcgc
     1141 aggaattaag cccatcggcg cggcggacgt gtggataggc aatccgaatg cgcccgacaa
     1201 gccgttccgt gccatgctga ttatccgcaa cgatgcgggc tatctgcgtt tgagcgagct
     1261 tttgacagag gcctatgtcg gcaaagaccg caatatccat catgcggaac tcaaccccga
     1321 atggctggaa aacggcgaca acagcggctt gatttgtttg agcggcgcgc attacggcga
     1381 agtgggcgtg aatctcttga acggcaatga agacgcggcg cgtgcggcgg cgttgaaata
     1441 tgcggcatgg tttcccgatg cgttttattt agagctgcaa cgcctgcccg aacgccccga
     1501 atgggaggct tgcgtttccg gcagcgtgaa gctggcagag gaactgagtt tgccggtggt
     1561 ggcgacccac ccgacgcagt ttatgagcag cgatgatttc aacgcgcacg aggcacgggt
     1621 gtgtattgcg ggcggctggg tgctgacgga caaaaaacgt ccgcgcgatt tcacaccggg
     1681 ccagtttttc attccgccgg aaacgatgct ggagcgtttc gccgatttgc ccgaggcctt
     1741 ggaaaacacg gtagaaattg ccaaacgctg caatctgcac atcacattgg gcaaaaactt
     1801 cctgcccctt ttccccacgc ccgacggttt atcgcgcgat gactatctca tcaaactctc
     1861 aaacaagggt ttgcaggaac gcatggttca gctttatccc gacgaggcgg agcgtgcggc
     1921 aaaaatgccg gaatatcagg aacgtttgga ttttgagctg aacatcatca tccagatgaa
     1981 attccccggc tatttcctta tcgtacaaga ctttatcaac tgggcgaaaa cgcacggctg
     2041 tccggtcggg ccgggacgcg gttcgggcgc gggttcgctg gtggcgtatt cgctgaaaat
     2101 taccgacctc gacccgctga aatacgcgct gctgtttgaa cgtttcttaa accccgaacg
     2161 cgtctccatg cccgactttg acgtggactt ttgccaatcc aaccgggggc gcgtgattga
     2221 gtatgtgcgc gaaaaatacg gcgcgaaggc agtcagccaa attgtcacct tcggcacgat
     2281 gtcgtccaaa gcagtgattc gcgacgtggg gcgcgtactg gaactgccgt ttatgctgtg
     2341 cgacaaactg tccaagctga ttccgttgga agccaacaaa cctctgggtt tggacgacgc
     2401 gatgaaggcg gagccgcaga ttcaggaatt aatcgaagcg gaagaagcgg acgaactgat
     2461 tacgctggcg aaaaagctgg aagacctgac gcgcggtttg ggtatgcacg caggcggcgt
     2521 gttgattgcg ccgggcaaga tttccgatta cagccccgtg tatcaggcgg atgaatccgc
     2581 ctctcccgta tccatgtacg acaagggcga cgtggaagac gtgggtttgg tgaaattcga
     2641 ctttttaggt ctgcgtaacc tgaccattat cgaaatggcg cagaacaaca tcaaaaacac
     2701 caccggcgac atcatcgatg tcggcaaaat tccgctcgac gaccaggccg cctaccaaat
     2761 cttccgcgat gcgaacacca ccgccgtgtt ccagttcgag tcgaccggca tgaaaaaaat
     2821 gctgaaaacg gcgcatacca ccaagtttga agaactgatt gccttcgtat cgctctaccg
     2881 cccaggcccg atggacaaca tccccgactt cgtcgcgcgt atgaaggggc aagaattcca
     2941 atacatccat ccgctgctcg agggcatcct cgcgccgacc tacggcatta tggtgtatca
     3001 ggaacaagtg atgcaggcgg cacagattat cggcggctac tcgctcggcg gcgcggacct
     3061 gctgcgccgc gcgatgggca agaaaaagcc cgaagaaatg gtgaaacacc gcgaaatctt
     3121 cgccgaaggc gcggcaaaac aaggcatttc gcgcgaaaaa tccgacgaaa tcttcaacta
     3181 catggaaaaa ttcgccggct acggtttcaa caaatcccac gctgccgcct acgccctgat
     3241 ttcctaccag accgcatggc ttaaagccca ctatcccgcc gaatttatgg cggcgaccat
     3301 gtcgtccgaa ttggacaaca ccgaccagct caagcatttc tacgacgact gccgcgccaa
     3361 cggcatcgag tttctgccgc ctgacatcaa tgaatccgac taccgcttca cgccgtatcc
     3421 ggacatgaaa atccgctacg cgctcggcgc gattaaaggt acgggcgaag ccgccgtcga
     3481 atccatcacc tccgcgcggc aaagcggcgg caagtttacc ggcctcttgg acttctgcga
     3541 gcgcgtcggc aaagaacaca tgaaccgccg cacccttgaa gccctgatac gcggtggcgc
     3601 gttcgacagc atcgaaccca accgcgccat gctcttggcg aacatcgacc tcgccatgaa
     3661 caacgccgac caaaaagccg ccaacaccaa tcagggcgga cttttcgaca tgatggaaga
     3721 cgccatcgaa ccggtgcggc tcgtcgacgc gccgatgtgg agcgaatcgg aaaaactcgc
     3781 cgaagaaaaa accgtcatcg gcttttacct gtccggccac ccgttcggcc cgtatgccca
     3841 agaagtccgc caaatcgccc cgcagaaact gtcgaaactc aaaccgcaag acagcgtgcg
     3901 cctcgccgga ttcgttaccg ccgtgcgtac gatgatgggc aagcgcggca aaatcgcctt
     3961 cgtcagcctt gaagatttga gcgggcaggt tgaaatcatg gtcggcggtc agacgctgga
     4021 aaactgcgcc gactgcctca aagccgacca agtgctgatt atcgaatcca aagtcagccg
     4081 cgacgactac ggcggcggcg acggactgcg catcatggca aaccaagtca tgaccctgca
     4141 aacggcacgc gaacgctacg cccgcagcct cagcctcgcc ctcgccccgc atcacgacat
     4201 cggcggactg gtacggctgc tctcctccca ccaactgccc gacacgccgc gcatcccgct
     4261 gcaactgtcg tatgccaacg aaaaagcgtc gggcaggctt caagtgccgc cgaaatggac
     4321 ggttacaccg agctccgcat tgttcggcga actggaaaca ttgctcggca gccggtcggt
     4381 gcgcgtcaac tggtaaccca aaatataaat gccgtctgaa gcccaaaatc ggtttcagac
     4441 ggcatttttt aaccgaaata aacgatgccc cttaaagcag aagctttaag gggcagagcg
     4501 ttgcggcaca tcttttcaga cggccttatt gtagcaacgg ttctcatttc gcagtgcgac
     4561 aatatgtttg ggattctagc cgttgtgaga tgataagtca ataatctatt tgtatataaa
     4621 taagacaata agatatatta tcattatttg caacgttgtt tacggagtaa taaatggctg
     4681 ccttcaaacc taatccaatc aactacatcc tcggcctcga tatcggcatc gcatccgtcg
     4741 gctgggcgat ggtagaaatt gacgaagatg aaaacccaat ctgcctgatt gatttgggcg
     4801 tgcgcgtatt tgagcgtgcc gaagtaccga aaacaggcga ctcccttgcc atggcaaggc
     4861 gtttggcgcg cagtgtccgc cgcctgaccc gccgtcgcgc ccatcgcctg cttcgggccc
     4921 gccgcctatt gaaacgcgaa ggcgtattac aagccgctga ttttgacgaa aacggcttga
     4981 tcaaatcctt accgaataca ccatggcaac ttcgcgcagc cgcattagac cgcaaactga
     5041 cgcctttaga gtggtcggca gtcttgttgc atttaatcaa acaccgcggt tatttgtcgc
     5101 aaagaaaaaa cgagggcgaa actgccgata aagagcttgg cgctttgctc aaaggcgtag
     5161 ccgacaatgc ccatgcctta cagacaggcg atttccgcac accggccgaa ttggctttaa
     5221 ataaatttga gaaagaaagc ggccatatcc gcaatcagcg cggcgattat tcgcatacgt
     5281 tcagccgcaa agatttacag gcggagctga ttttactgtt tgaaaaacaa aaagaatttg
     5341 gcaatccgca tgtttcaggc ggccttaaag aaggtattga aaccctactg atgacgcaac
     5401 gccctgccct gtccggcgat gccgttcaaa aaatgttggg gcattgcacc ttcgaaccgg
     5461 cagagccgaa agccgctaaa aacacctaca cagccgaacg tttcatctgg ctgaccaagc
     5521 tgaacaacct gcgtatttta gagcaaggca gcgagcggcc attgaccgat accgaacgcg
     5581 ccacgcttat ggacgagcca tacagaaaat ccaaactgac ttacgcacaa gcccgtaagc
     5641 tgctgggttt agaagatacc gcctttttca aaggcttgcg ctatggtaaa gacaatgccg
     5701 aagcctcaac attgatggaa atgaaggcct accatgccat cagccgtgca ctggaaaaag
     5761 aaggattgaa agacaaaaaa tccccattaa acctttctcc cgaattacaa gacgaaatcg
     5821 gcacggcatt ctccctgttc aaaaccgatg aagacattac aggccgtctg aaagaccgta
     5881 tacagcccga aatcttagaa gcgctgttga aacacatcag cttcgataag ttcgtccaaa
     5941 tttccttgaa agcattgcgc cgaattgtgc ctctaatgga acaaggcaaa cgttacgatg
     6001 aagcctgcgc cgaaatctac ggagaccatt acggcaagaa gaatacggaa gaaaagattt
     6061 atctgccgcc gattcccgcc gacgaaatcc gcaaccccgt cgtcttgcgc gccttatctc
     6121 aagcacgtaa ggtcattaac ggcgtggtac gccgttacgg ctccccagct cgtatccata
     6181 ttgaaactgc aagggaagta ggtaaatcgt ttaaagaccg caaagaaatt gagaaacgcc
     6241 aagaagaaaa ccgcaaagac agggaaaaag ccgccgccaa attccgagag tatttcccca
     6301 attttgtcgg agaacccaaa tccaaagata ttctgaaact gcgcctgtac gagcaacaac
     6361 acggcaaatg cctgtattcg ggcaaagaaa tcaacttagg ccgtctgaac gaaaaaggct
     6421 atgtcgaaat cgaccatgcc ctgccgttct cgcgcacatg ggacgacagt ttcaacaata
     6481 aagtactggt attgggcagc gaaaaccaaa acaaaggcaa tcaaacccct tacgaatact
     6541 tcaacggcaa agacaacagc cgcgaatggc aggaatttaa agcgcgtgtc gaaaccagcc
     6601 gtttcccgcg cagtaaaaaa caacggattc tgctgcaaaa attcgatgaa gacggcttta
     6661 aagaacgcaa tctgaacgac acgcgctacg tcaaccgttt cctgtgtcaa tttgttgccg
     6721 accgtatgcg gctgacaggt aaaggcaaga aacgtgtctt tgcatccaac ggacaaatta
     6781 ccaatctgtt gcgcggcttt tggggattgc gcaaagtgcg tgcggaaaac gaccgccatc
     6841 acgccttgga cgccgtcgtc gttgcctgct cgaccgttgc catgcagcag aaaattaccc
     6901 gttttgtacg ctataaagag atgaacgcgt ttgacggtaa aaccatagac aaagaaacag
     6961 gagaagtgct gcatcaaaaa acacacttcc cacaaccttg ggaatttttc gcacaagaag
     7021 tcatgattcg cgtcttcggc aaaccggacg gcaaacccga attcgaagaa gccgataccc
     7081 cagaaaaact gcgcacgttg cttgccgaaa aattatcatc tcgccccgaa gccgtacacg
     7141 aatacgttac gccactgttt gtttcacgcg cgcccaatcg gaagatgagc gggcaagggc
     7201 atatggagac cgtcaaatcc gccaaacgac tggacgaagg cgtcagcgtg ttgcgcgtac
     7261 cgttaacaca gttaaaactg aaagacttgg aaaaaatggt caatcgggag cgcgaaccta
     7321 agctatacga agcactgaaa gcacggctgg aagcacataa agacgatcct gccaaagcct
     7381 ttgccgagcc gttttacaaa tacgataaag caggcaaccg cacccaacag gtaaaagccg
     7441 tacgcgtaga gcaagtacag aaaaccggcg tatgggtgcg caaccataac ggtattgccg
     7501 acaacgcaac catggtgcgc gtagatgtgt ttgagaaagg cgacaagtat tatctggtac
     7561 cgatttacag ttggcaggta gcgaaaggga ttttgccgga tagggctgtt gtacaaggaa
     7621 aagatgaaga agattggcaa cttattgatg atagtttcaa ctttaaattc tcattacacc
     7681 ctaatgattt agtcgaggtt ataacaaaaa aagctagaat gtttggttac tttgccagct
     7741 gccatcgagg cacaggtaat atcaatatac gcattcatga tcttgatcat aaaattggca
     7801 aaaatggaat actggaaggt atcggcgtca aaaccgccct ttcattccaa aaataccaaa
     7861 ttgacgaact gggcaaagaa atcagaccat gccgtctgaa aaaacgcccg cctgtccgtt
     7921 aaattaatct atccctgttt caggcggcct gaagcaggga ttttttatat aaaaataagg
     7981 ggaaaaacat gacttggcgc agcctgctta ttcaaaacgg cggcaaacta tccttgcaac
     8041 gccggcaact gctgattcaa caaaacggcg agtcccacac cgtaccatta gaggacatcg
     8101 ccgttatcat catcgaaaat cgcgaaaccc tgattaccgc cccgctccta tcagccttag
     8161 ccaagcatgg tgcaaccctg ctgacctgcg acgagcagtt cctgccctgc ggacaatggc
     8221 tgccttatgc ccaatatcac cgccaactta aaatcctcaa actgcaactg aacataagcg
     8281 aacccttaaa aaagcaactc tggcaacata tcgtccgcca aaaaatcctg aaccaagcat
     8341 ttgtcgcaga cgaaacaggc aatgatcttg cagccaaacg gctgcgcact ttggcatctg
     8401 aagtccgttc aggcgataca ggaaaccgtg aagcccaagc tgccgccctc tactttcaag
     8461 ccctctttgg cgaaaaattt acccgtaatg acaacaacgc cgtcaatgcc gcattgaact
     8521 acacctacgc cgtactccgt gccgcagttg cacgcgccct gaccttatac ggttggttac
     8581 ctgcattagg cttgtttcat agaagtgaac tcaatccatt taatctcgcc gatgacttta
     8641 tcgaaccctt acgcccattg gccgacctga ccgtgataca tttgtacgaa caaggccgtc
     8701 tgaaaactga actgacgctc ggcatcaaac aacacctcat caaaatcctc tactatcaaa
     8761 ccagcatcga acggcaacat ttcagcacct tggctgccat cgacaaaatg atttcgtcat
     8821 ttcaagccgg cgtaaccgat aaaaacgcca aacaactgaa actgcctgaa atcctaccat
     8881 tgaaagaata ccaatatgag tgaggccaaa tttatgagga ttatcgtctt cttcgaccta
     8941 ccggttatca cggcggcaaa gcgcaaagcc gccaatcaat tccgccagtt tttattaaaa
     9001 gacggatacc aaatgctgca actttccgta tacagccgta tcgtcaaagg ccgcgattcg
     9061 ttgcaaaaac accacaacag actgtgtgca aaccttccgc aagaaggctc aatccgctgt
     9121 ttggagataa cagaaaagca atatgccgcc atgaaactgc tgttgggcga gctgaaaacc
     9181 caagaaaaaa aggtcaattc agaccaatta ttgttatttt aagcccattt tttcataaca
     9241 aataaaacgg gaaaccctta tgaaataagg atttcccgtc gaagtattgt agcactgcga
     9301 aatgagaaag ggagctacaa cactatcgag ggtacattcc ctatcagtgt tattgtagca
     9361 ctgcgaaatg agaaagggag ctacaacgcc ccgaatgtga ttttacgctt tggaaaaatt
     9421 gtagcactgc gaaatgagaa agggagctac aacctttttg catcttcaat aacctcattt
     9481 acaattgtag cactgcgaaa tgagaaaggg agctacaacg gccggaaagg gtgggcgata
     9541 aatggattta ttgtagcact gcgaaatgag aaagggagct acaaccacct ttaaatttgc
     9601 ctgacacttt gacctattgt agcactgcga aatgagaaag ggagctacaa cccatgaaaa
     9661 cctcatgcat cgatctatga aattgtagca ctgcgaaatg agaaagggag ctacaacgga
     9721 ggcgaccagc gtaccaagta cgcaggcatt gtagcactgc gaaatgagaa agggagctac
     9781 aacattcggg tcgcaacaga aacctttacc aaaattgtag cactgcgaaa tgagaaaggg
     9841 agctacaaca caaaggctta taacttcttc aatgaagaga ttgtagcact gcgaaatgag
     9901 aaagggagct acaacaaatc atcaagtgct ttcatagaca atcgaattgt agcactgcga
     9961 aatgagaaag ggagctacaa ccctgtcagg agatgggcaa accttcagac gattgtagca
    10021 ctgcgaaatg agaaagggag ctacaaccat tttttacggc attttatata catcattatt
    10081 gtagcactgc gaaatgagaa agggagctac aacgtaaagt tagtgcggtt gtatcttcat
    10141 cagattgtag cactgcgaaa tgagaaaggg agctacaacc acatttgatt ggaacggcgg
    10201 agattgtgta ttgtagcact gcgaaatgag aaagggagct acaaccaacg atttggcgga
    10261 tgtatttgag gctgaattgt agcactgcga aatgagaaag ggagctacaa cgatgggaac
    10321 ttagttctta aatctttctc aattgtagca ctgcgaaatg agaaagggag ctacaactgg
    10381 gcgtaatgcc tgttgtcgct ggtagcaatt gtagcactgc gaaatgagaa agggagctac
    10441 aactcgtgca ggattcggtt ttccacctcc caaattgtag cactgcgaaa tgagaaaggg
    10501 agctacaacc agtatatcga gttgcggaat atcagggtga ttgtagcact gcgaaatgag
    10561 aaagggagct acaactcggt caggtcatag acggctttgt tgtcgattgt agcactgcga
    10621 aatgagaaag ggagctacaa ctcaaacctt cgttgtaaag tcggtctgat tattgtagca
    10681 ctgcgaaatg agaaagggag ctacaacagc agaagcagac caaaaaagaa acgcttaatt
    10741 gtagcactgc gaaatgagaa agggagctac aaccgaactg caagaactcc attcacgacc
    10801 cctattgtag cactgcgaaa tgagaaaggg agctacaaca aaccagccga tgccgccttt
    10861 aaaagtgata ttgtagcact gcgaaatgag aatgggagct acaacactca aattcagtct
    10921 gtcaacccgt tccgcattgt agcactgcga aatgagaatg ggcgctacaa ccagccgttg
    10981 cgataagcga acgtgcgtta aagtcattga gacacagtgc aattatggca agaaaggcga
    11041 cagatattgt gtcacagatt tgaaataaga aaaaaccgcc cactcacaga gtaggcggaa
    11101 aacttgcgtt tttttgattg tggtcgggtt agctactgca ttgacttaac agcaaagcaa
    11161 ccgctaaaat gaacttgtgg ttcacttcaa cggacagcag caactttttc ccacgcataa
    11221 ggaatttaag cattttagct tctcctagtg aagttaatca cacaggcgag agccgaacaa
    11281 aaatccccgt tgcagcgggg atttttgctg ttccctgttt gatgattaaa ctaaaatctt
    11341 gcaaaatgag acctttgcaa aattccccaa aatcccctaa attcccacca agacatttag
    11401 gggatttctc atgagcacct tctttcaaca aaccgcccaa gccatgattg ccaaacacat
    11461 cgaccgtttc ccgctattga agttggacca ggtgattgat tgacagccga tcgagcagta
    11521 cctgaaccgt caaaaaaacc gttaccttag agaccaccgc ggccgtcccg cctatcccct
    11581 gttgtccatg ttcaaagccg tcctgctcgg acaatggcac agcctctccg atcccgaact
    11641 cgaacacagc ctcattaccc gcatcgattt caacctgttt tgccgtttcg acgaactgag
    11701 c
//
LOCUS       DAWFBO010000054        11696 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFBO010000054 DAWFBO010000000
VERSION     DAWFBO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.054"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(308..730)
                     /locus_tag="WGO87_001025"
     CDS             complement(308..730)
                     /locus_tag="WGO87_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0392394.1"
                     /translation="MQVTSKWIDGMCFVGTTESGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(848..1519)
                     /locus_tag="WGO87_001026"
     CDS             complement(848..1519)
                     /locus_tag="WGO87_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0392395.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1535..2161)
                     /locus_tag="WGO87_001027"
     CDS             complement(1535..2161)
                     /locus_tag="WGO87_001027"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0392396.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2268..3074)
                     /locus_tag="WGO87_001028"
     CDS             complement(2268..3074)
                     /locus_tag="WGO87_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0392397.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     TKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3333..3773)
                     /locus_tag="WGO87_001029"
     CDS             complement(3333..3773)
                     /locus_tag="WGO87_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0392398.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3836..5242)
                     /locus_tag="WGO87_001030"
     CDS             complement(3836..5242)
                     /locus_tag="WGO87_001030"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0392399.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5412..6527
                     /gene="lptF"
                     /locus_tag="WGO87_001031"
     CDS             5412..6527
                     /gene="lptF"
                     /locus_tag="WGO87_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0392400.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6524..7594
                     /gene="lptG"
                     /locus_tag="WGO87_001032"
     CDS             6524..7594
                     /gene="lptG"
                     /locus_tag="WGO87_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0392401.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7904..10489
                     /gene="acnB"
                     /locus_tag="WGO87_001033"
     CDS             7904..10489
                     /gene="acnB"
                     /locus_tag="WGO87_001033"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0392402.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10631..11626
                     /locus_tag="WGO87_001034"
     CDS             10631..11626
                     /locus_tag="WGO87_001034"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0392403.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2685 a         3258 c         3041 g         2712 t
ORIGIN      
        1 aataaattaa tatatggctt aaaataacgg gatgcccgcc ttccgcccgc ccgcagaagc
       61 aggcgggtat cattttaaaa cgcggcattt aaaatttgac cgaaaattgt tgacaatccg
      121 gaatcaagtc tgcacaatac cccgacaagt ccaagtatta taaaggctga ataaagagga
      181 aacagcaggc agatatattc gggaggtgca gtccgaatat atctgctttt ttatgcgcct
      241 ccggattgcc tgccgcacct ttcccttcag acggcatcag ccgtttcccc ataatgccgc
      301 ccgatgccta tttatctgcc ccggcaattt caaaactgtg ggtaatcttt gccgctttgc
      361 ccaacataat cgaagccgaa cagtattttt cggcagacat ctgaacggcg cgctcaatgg
      421 ccgattcttt caaatcatgc ccgaatactt tgaaatggat gtggatttcg gtaaacacgc
      481 gcggcgcatc gtccgcccgt ttcgccgtaa ccgtcgcacg gcagtcagtc actttctgac
      541 gctgtttttc ggcaatcatc accacatcga tgctcgaaca gcccgccacg cccaacagca
      601 gcatttccaa agggctgggc ccgcgcttgg ccgcaccttc tgccgccgaa ccctccataa
      661 cgacgctgtg cccgctttcc gtcgtgccga caaagcacat tccgtctatc cattttgatg
      721 taacctgcat ggtgtcattc ctgaaaatag cgttaaaacc gctttgcata tggcgttatt
      781 gtaaacaatt tcaagcggct tatgcagaaa tatggacaaa acggcgaaaa aacgcctgaa
      841 aaccgattta cggtttggcg gactggccat tgatctgcac cgatttgagt ttcagcgtat
      901 aggttttgcc gtcgtcggta tagccgattt gtgccggaat attgttcagg gacggtgcga
      961 agaaatacat taccgtatcg tcgccgcgcc gcacccgata tttgacgact tcggtttcca
     1021 cgccgcctat gctgtatttt cctgtacccg ccttattcaa accgccgacg gaataaagtt
     1081 ttttgccgtt ggtgattttc agccccgggg ggagtttcgc gtcatttgcc gccaactgcc
     1141 aggcaagcgt gaacaaatcc atagccttgg ggctttgctc ggttttgctc tcgcccgctt
     1201 tgccgtaggt tacgctgccg tcggcgaatt tggcttccgc atacagtttg cccctgcgta
     1261 tgtctctata gtaggtaggg tgcagggtat tgccgacaac cgtaccgccg gactcgaaac
     1321 ggatattgta tagcggcact ttaatcgtcg aaacgatttt gtaagcattg ccgctgcgtt
     1381 caaatgtcat cgtggcggga atgccgtagc tgccggaata gtgcagcacg gcggattggg
     1441 gcagccctgc cgcatacgcg cacggcaggg cggcggacaa aatggcggcg gaaaatatat
     1501 ttttaaaagt cttcatcatt tgctcccgcc cggtttacgc cgtcagaaaa cgggcggcat
     1561 cggcgttttc cgaatttctg acgcggtttc cctcgataat caggcggccg gcggcaaaat
     1621 cggcaacggc tttcggatag agtttatgct cgacagccaa aacccgggcg gcaacatcgt
     1681 ctgccgtatc gccgtcgagt atcggcacaa ccccttgcga tacaatcggg ccgcaatcca
     1741 gttcggcagt aacgaaatgg atggtgcagc cggcaacgcg acaacctgcc tccaaagcgc
     1801 gttcgtgcgt atgaagtccg gtaaacgagg gaaggatgga cgggtgaatg ttcatcagcc
     1861 tgccttcgta atgggcacag aactcagggg tcagaatccg cataaaacct gccaaaacca
     1921 ccaagtcggg ttgatatgcg tcgatttttt ccatcatggc ggtatcgaag gcaagtcgtg
     1981 attcaaagtt tttatgattc aggctgtcgg tcggaatacc ctgaccggcc gcccattgca
     2041 aaccggcagc cgtttcgctg ttgctcaaca cggcggcaat acggacgttg ggaatggcgg
     2101 cattgacgat tgcctgcata ttgctgccgc gtccggaaat caggatgacg atgtttttca
     2161 taatggtgcg cttttgaaag ggatgccatc tgaaccgctg tttggtggtt tcagacggca
     2221 tttgccgtaa aaatgcccga aaacctgttt cgggcatgga ttcggactta atttactttt
     2281 ttgatgtcga cttgagccgg ctgcttggcg ggcgcgtttt cgggtgcgcc gattttgacc
     2341 agtttcacat caaataccaa agtggcgttc gggccgattt tgtcgcccgc accctgttcg
     2401 cggtaggcaa ggttggacgg gatgtagaac gtggcttcgc cgccttcttt cagaagctgt
     2461 acgccttcgg tccaacccgg aatcacttgg ctcaaaggga aggtgaccgg gccgccgttg
     2521 gctttgctgc tgtcgaatac cgtaccgtca atcaggcggc cttcgtattc cacggtaacg
     2581 atgtcgtctt tggtcggctg tttgccttcg ccctgtttgg tgattttgta ttgcaggccg
     2641 gaagcagtgg tcttcacgcc gtctttggcg gcattttctt tcagaaaggc ttcgcctttt
     2701 tctttattgg ccttcgcgtc cgccttgtgt ttttctacgg ctttagcctg ttgttcctga
     2761 aggaatttca tcatgacttc ctgagcctgc tcttcagtca ttttgatttc tttgccgtca
     2821 tacactgcct gcatggcttc ggtaaagact ttcaaatcga tttccgcacc ctgttccttc
     2881 atttgcttca gggagcgtcc gatgtccacg cccatcgcat agcttgcctg ctgcatcgtg
     2941 ctgccgatcg aagaggtgtc gccctgcgcg gcagaagcgg cggcaggttc ggatgcggct
     3001 tcttttttgc cacaggcgga aagtgccaaa gcggcggaaa gggtcagtgc gctgattttg
     3061 aaaatggtgt tcatgatgga tcttcgctgt cgataaggtc ggaaaaacgg gattatagcc
     3121 gagtttgaat gtttcaacac acaggatgac acataaagcg tcaatcgtgt gttgccctgt
     3181 tttggaaggg attgaacctt ccaaaataag ttttgattct accgccccga gggacagatg
     3241 tccaagtggc ggggttcaac cgataaggaa attttaatca aatagaatca agcctgttta
     3301 aattttgtaa atgcggcatt tcagacggca ttttatgcct tgccctccat gccgtgatgt
     3361 tcgatggcaa aaccgcttcg gcggtaggcg gtaaagcgtt cgcgcgcgtc ggcgagatct
     3421 tccaagctgt tgccgacgat ttccaaaacg cgcgcgggca agaccggagc ggtgttccaa
     3481 aaaccgtcgg acaggttcaa aacagtcatg ccttcgggaa ttcggggcgg attgccgccg
     3541 caagcaagca ggacggatgt ttcagacggc atggcttctt ccatttccca aatttcgtgc
     3601 ggaatgaaac tctcggcttc gtattgccaa agcattttgt ccaattcctg aagccgcccg
     3661 aacgaatcgg accacaccag tatcctgccg ccgtcccgaa tcgcacgggc aatcaggcgg
     3721 caggtgaaaa tcggaacttg ggcaacgtgc gtgtaaaagg tggctttcgg catattgttt
     3781 gagcatttgg caggacgatg ccgtctgaaa ggcttcagac ggcattgtgg caaaattaaa
     3841 gattccgcag atagttcagc agcaagggaa cgggacggcc agtcgcacct ttttccgctc
     3901 cggatttcca cgccgtaccc gcgatgtcaa ggtgcgccca cgggtagcct tcggtgaagt
     3961 aagacaggaa tgttgcggcg gtaatcgtgc ccgcgccggg cgtgccgatg tttggaatgt
     4021 cggcaaagtt ggatttgagt tggtctttgt aggtctcaaa gagcggcagt tgccatgctt
     4081 tgtcgtccac gttgtaggaa gcggcaagca ggctgtcgat caaatcctga ttgttgccca
     4141 tcacgccgct gacatcgtgc cccaaggcaa caatacacgc gccggtcagg gtggcgacgt
     4201 cgatgacggc tttgggtttg aactgctcgg cgtaagtgag cgcgtcgcac aaaatcagac
     4261 ggccttcggc atcggtgttc aacacttcga tggtcagacc tttcatactt ttcacgacat
     4321 cgcccggttt gtttgccgcg ccggaaggca tattttcaca agtggcgacg acggcaatca
     4381 ggttaatcgg cagttgcagt ttgacggcgg cgcagaaggt gctgatgacg gttgccgcgc
     4441 cgcacatatc aaacttcatt tcgtccatgt tcaggccggg cttgagggag atgccgccgg
     4501 tgtcgaaggt aatgcctttg ccgaccaata ccacaggcgc ggcttctttg tcggctgcac
     4561 cgaaatagct cagttcgacc aaataggggg cttccgcgct gcctttggcg accgaccaaa
     4621 acgaacccat gttttctttg atgtagtctt tttcgatgat tttggcgtgc gcgcccagtt
     4681 tttcggcttc ggctttggcg gtgcgcgcta aaaattcggg cgtgcattcg ttgggcgcgg
     4741 cgttgcccaa gtcgcggcag aggctttgtc cgtaaacttg cgcttcggcg acgcgcaagg
     4801 cttctttaac gacggcttcg tgtgcgctgt ggaacacggc ggtttcaaat ttggcgggct
     4861 tggcttcttt tttgtagcgg tcgaaacggt aggcggcgtt gccgaacgca atcgcaaacg
     4921 cttcggctac ggcggcggct tgcgcttcat cgaaggcatg gatgtccaca ttgaccgttt
     4981 cctgattttg cgcccatttg gcggcttcgg cggcggcctt gttcaatgcg gcgcggccgg
     5041 tgcttttcag acagcatacg gcaacagcct gcaaaccgtt gcctgtcggg atttttgtgt
     5101 cggcaaaatt ttgaccttct tcaagcgaag acaaaagggc aagggcggtc ggatggttta
     5161 attgcgcctc ttcggtgcag acaaataact gtgcgcctgc ctgctgttcc tgcaagattt
     5221 cggtttttgt gctaaattcc acgtttattc tcctgattga gacggttgtc ggtagttttc
     5281 ggacggcctt tcgctcaaaa gaccgtctga agacggctgg cacgattgta ccccatttga
     5341 agcaccgtct gaaaccttgc gcggacaatc cgcctgcgcc gaaccgctta ccgccccctg
     5401 accgcgattc tatgatttat caaagaaacc tcatcaaaga actctctttt accgccgtcg
     5461 gcattttcgt cgtcctcttg gcggtattgg tctccacgca ggcaatcaac ctgctcggcc
     5521 gtgccgccga cgggcgtgtc gccatcgatg ccgtgttggc attggtcggc ttctgggtca
     5581 tcggtatgac gccgcttttg ctggtgttga ccgcatttat cagtacgttg accgtgttga
     5641 cccgctactg gcgcgacagc gaaatgtcgg tctggctatc ctgcggattg gcattgaaac
     5701 aatggatacg cccggtgatg cagtttgccg tgccgtttgc cgttttggtt gccgtcatgc
     5761 agctttgggt gataccgtgg gcagagctac gcagccgcga atacgctgaa atcctgaagc
     5821 agaagcagga attgtctttg gtggaggcag gcgggttcaa cagtttgggc aagcgcaacg
     5881 gcagggttta ttttgtcgaa accttcgata ccgaatccgg catcatgaaa aacctgttcc
     5941 tgcgcgaaca ggacaaaaac ggcggcgaca acatcatctt cgccaaagaa ggtaacttct
     6001 cgctgaacga caacaaacgc acgctcgaat tgcgccacgg ctaccgttac agcggcacgc
     6061 ccggacgcgc cgactacaat caggtttcct tccaaaaact caacctgatt atcagcacca
     6121 cgccaaaact catcgacccc gtttcccacc gccgtaccat tccgaccgcc caactgattg
     6181 gcagcagcaa cccgcaacat caggcggaat tgatgtggcg catctcgctg accgtcagcg
     6241 tcctcctact ctgcctgctt gccgtgccgc tttcctattt caacccgcgc agcggacata
     6301 cctacaatat cttgattgcc atcggtttgt ttttaattta ccaaaacggg ctgaccctgc
     6361 tttttgaagc cgtggaagac ggcaaaatcc atttttggct cggactgctg cctatgcaca
     6421 tcatcatgtt cgtcatcgca atcgtacttc tgcgcgtccg cagcatgccc agccagccct
     6481 tctggcaggc ggttggcaaa agtctgacat tgaaaggcgg aaaatgaacc tgatttcacg
     6541 ttacatcatc cgtcaaatgg cggttatggc ggtttacgcg ctccttgcct tcctcgcttt
     6601 gtacagcttt tttgaaatcc tgtacgaaac cggcaacctc ggcaaaggca gttacggcat
     6661 atgggaaatg ctgggctaca ccgccctcaa aatgcccgcc cgcgcctacg aactgatgcc
     6721 cctcgccgtc cttatcggcg gactggtctc tctcagccag cttgccgccg gcagcgaact
     6781 gaccgtcatc aaagccagcg gcatgagcac caaaaagctg ctgttgattc tgtcgcagtt
     6841 cggttttatt tttgctattg ccaccgtcgc gctcggcgaa tgggttgcgc ccacactgag
     6901 ccaaaaagcc gaaaacatca aagccgcggc catcaacggc aaaatcagta ccggcaatac
     6961 cggcctttgg ctgaaagaaa aaaacagcat tatcaatgtg cgcgaaatgt tgcccgacca
     7021 taccctgctg ggcattaaaa tctgggcccg caacgataaa aacgaactgg cagaggcagt
     7081 ggaagccgat tccgccgttt tgaacagcga cggcagttgg cagttgaaaa acatccgccg
     7141 cagcacgctt ggcgaagaca aagtcgaggt ctctattgcg gctgaagaaa actggccgat
     7201 ttccgtcaaa cgcaacctga tggacgtatt gctcgtcaaa cccgaccaaa tgtccgtcgg
     7261 cgaactgacc acctacatcc gccacctcca aaacaacagc caaaacaccc gaatctacgc
     7321 catcgcatgg tggcgcaaat tggtttaccc cgccgcagcc tgggtgatgg cgctcgtcgc
     7381 ctttgccttt accccgcaaa ccacccgcca cggcaatatg ggcttaaaac tcttcggcgg
     7441 catctgtctc ggattgctgt tccaccttgc cggacggctc ttcgggttta ccagccaact
     7501 ctacggcatc ccgcccttcc tcgccggcgc actacctacc atagccttcg ccttgctcgc
     7561 cgtttggctg atacgcaaac aggaaaaacg ctaaaccaat gccgtctgac ccccttcgga
     7621 cggcattggt ttttattatc atattcctgt agacagatag ccgttcccta ttatagtgga
     7681 ttaacaaaaa ccagtacggc gttgcctcgc cttgccgtac tattgtactg tctgcggctt
     7741 cgtcgccttg tcctgatttt tgttaatcca ctatacatta cctgtcataa cagttccatt
     7801 tttgttaaaa ctagtctatg atagcggtac aaatattgtt tacaatattt aacgcaaatc
     7861 atttgcaacc cgacaaaaga aaaacagaaa aaggaacaaa gagatgttag aagcctatcg
     7921 taaagccgcc gccgagcgcg ccgccctcgg cattcccgcc ctccctttga acgcgcagca
     7981 aaccgccgat ttggttgagc tgctgaaaag cccgcccgca ggcgaaggcg agttcttggt
     8041 cgaactgctt gcccaccgtg ttccgcccgg tgtggacgat gccgccaaag tcaaagcctc
     8101 attcctggct gccgttgccg aaggcagcgc gtccagcccg ctgatctccc ccgaatatgc
     8161 gaccgaactc ttaggtacga tgctcggcgg ttacaatatc cacgccttaa tcgaactctt
     8221 ggacgacgac aaactcgcgc ccattgccgc caaaggcttg aaacatacgc ttctgatgtt
     8281 cgattccttc cacgacgttc aggaaaaagc cgaaaaaggc aacaaatacg cgcaagaagt
     8341 tttgcaatct tgggcagatg ccgaatggtt cgcctcacgc gccaaagttc ccgaaaaaat
     8401 caccgttacc gttttcaaag ttgacggcga aaccaataca gacgacctct cccccgcgcc
     8461 cgacgcgtgg agtcgtcccg atattccgct gcacgcgctg gcaatgctga aaaacccgcg
     8521 cgacggcatc acgcccgaca aaccgggcga agtcggtccg attaaattgt tggaagaact
     8581 caaagccaaa ggccatccgg ttgcttacgt cggcgacgtg gtcggtactg gttcttcacg
     8641 caaatccgcg accaactccg tcatttggca taccggcgaa gacattccgt tcgtgccgaa
     8701 caaacgcttc ggcggcgtat gtttgggcgg caaaatcgcg ccgattttct tcaataccca
     8761 agaagattcc ggcgcgctgc cgattgaagt cgatgtatct gctctaaaaa tgggcgatgt
     8821 cgtcgatatc ctgccttatg aaggcaaaat cgtgaaaaac ggcgagactg ttgccgagtt
     8881 tgaattgaaa tcacaagtat tgctggacga agtgcaagcc ggcggccgta tcaacctgat
     8941 tatcggccga ggtctgaccg ccaaagcgcg cgaagccctg aaactgcctg cctctactgc
     9001 attccgcctg ccgcaagcgc ctgccgaaag caaagccggt ttcaccttgg cgcaaaaaat
     9061 ggtcggccgc gcctgcggtc tgcccgaagg acaaggcgtg cgcccgggta cttactgcga
     9121 accgcgtatg acgacggtcg gctcgcaaga cacgaccggc ccgatgaccc gcgacgagtt
     9181 gaaagacttg gcttgtttgg gcttctccgc cgatatggtg atgcagtctt tctgccacac
     9241 cgccgcctat ccgaaacctg tcgatgtaaa aacccataaa gaactgcccg cctttatttc
     9301 cacccgtggc ggcgtgtcac tgcgtccggg cgacggcgtc atccactcgt ggctcaaccg
     9361 cctgctgctg cccgataccg tcggcaccgg cggcgacagc catacccgtt tccccatcgg
     9421 tatttccttc cccgccggct ccggcttggt tgcctttgcc gccgcaacgg gcgtaatgcc
     9481 gctcgatatg cccgagtctg tattggtacg cttcagcggc aagctgcaac cgggcgtaac
     9541 cctgcgcgat ttggtgaacg ccatcccgct gtacgcaatt aaacaaggtt tgctgaccgt
     9601 tgccaaagcc ggtaagaaaa acatcttctc tggccgcatc ctcgaaatcg aaggcctgcc
     9661 cgatttgaaa gtggaacaag cctttgaatt gaccgacgca tccgccgaac gctccgccgc
     9721 cggctgtacc gtgaagctca ataaagagcc gattatcgag tacatgaaat ccaacgtcgt
     9781 gttgatgaaa aacatgattg ccaacggcta tcaagacccg cgcactttgg aacgccgcat
     9841 caaagccatg gaaaaatggc tggcaaatcc cgagttgctc gaagcggata aagatgccga
     9901 atacgccgcc gtgattgaaa tcaacatgga cgacatcaaa gagccgatta tcgcctgccc
     9961 gaacgacccg gacgacgtgt gcttcatgtc cgaacgctcc ggcaccaaaa tcgacgaagt
    10021 attcatcggt tcgtgtatga ccaacatcgg ccacttccgc gccgcctcca aacttttgga
    10081 aggcaaggca gacacccccg tccgcctgtg gattgcgccg ccgaccaaaa tggacgcgaa
    10141 acaattgtcc gacgaaggac actacggcgt actcggacgt gccggcgcgc gtatggaaat
    10201 gccgggttgc tccttatgta tgggtaatca ggcgcaagta cgcgaaggtg cgaccgttat
    10261 gtccacctcc acccgcaact tcccgaaccg tttgggtaaa aacacctttg tttacctcgg
    10321 ttcggcggaa ttggcagcga tttgctccaa actgggtaaa atcccgaccg ttgaagaata
    10381 tcaagccaat atcggcatca tcaacgaaca gggcgataaa atctaccgct atatgaactt
    10441 caacgaaatc gacagctaca acgaagtagc cgagaccgtg aacgtttaat ccccgtcatc
    10501 cgtatgaagt aagggattga ccgcaatgcc gtctgaacaa ccttcagacg gcattgcaac
    10561 attccgctaa cccttctttc cgcaaacgct gcaaatacgg cgttcacgcc cccacataaa
    10621 ggaaacgaca gtgaacctga aaaaccgcca ttttctgaaa cttttagact tcacgccgga
    10681 agaaatcacc gcctacctcg accttgccgc cgaattgaaa gccgccaaaa aagcagggcg
    10741 cgagattcag cggatgaaag ggaaaaacat cgccctgatt tttgaaaaaa cctctactcg
    10801 gacgcgctgc gcgtttgaag tcgccgcgcg cgatcaaggc gcgggagtga cttatttaga
    10861 gccgtccgcc agccaaatcg ggcataagga aagcatcaaa gacaccgccc gcgtgttggg
    10921 caggatgtac gatgccatcg aatatcgcgg tttcggtcag gaagttgttg aagaattggc
    10981 gaaatacgcg ggcgtacccg tgttcaacgg gctgaccaac gagttccatc ccacacaaat
    11041 gcttgccgac gcactgacta tgcgcgaaca cagcggcaaa cctttgaacc aaaccgcgtt
    11101 tgcctacgtc ggcgacgcgc gttacaacat gggcaattcc ctgctgattt taggggcaaa
    11161 attggggatg gacgtgcgta tcggcgcacc gcaaagcctg tggccgtctg aaggcattat
    11221 tgccgccgca cacgccgccg ccaaagaaac cggcgcaaaa attaccctga ccgaaaacgc
    11281 gcatgaagcc gtgaagaatg ttgattttat tcataccgat gtgtgggtca gcatgggcga
    11341 gccgaaagaa gtctggcagg aacgcatcga tttgctgaaa gattaccgcg ttacgcccga
    11401 actgatggcg gcatcgggca atccgcaagt caaattcatg cactgcctgc ccgccttcca
    11461 caaccgcgaa accaaagtcg gcgaatggat ttacgaaacc ttcgggctga acggtgtgga
    11521 agttacagaa gaagtattcg aaagccccgc cagcatcgtg ttcgatcagg cggaaaaccg
    11581 tatgcacacg attaaagcgg taatggtcgc ggctttgggc gactgacaga actgtgcctg
    11641 tttaaattca tccgcaacac agataccgtc tgaacacgat gttcagacgg tatcca
//
LOCUS       DAWFBO010000055        11680 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFBO010000055 DAWFBO010000000
VERSION     DAWFBO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.055"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            125..1117
                     /gene="pheS"
                     /locus_tag="WGO87_001035"
     CDS             125..1117
                     /gene="pheS"
                     /locus_tag="WGO87_001035"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0392404.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            1294..2358
                     /locus_tag="WGO87_001036"
     CDS             1294..2358
                     /locus_tag="WGO87_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0392405.1"
                     /translation="MIKSFELEKFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQATWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            2365..2883
                     /locus_tag="WGO87_001037"
     CDS             2365..2883
                     /locus_tag="WGO87_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392406.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVLLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            2919..5282
                     /locus_tag="WGO87_001038"
     CDS             2919..5282
                     /locus_tag="WGO87_001038"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0392407.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     CVFEIARVFSKGSDGQFVQNERIGGLWYGAVMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGETRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            5356..5658
                     /locus_tag="WGO87_001039"
     CDS             5356..5658
                     /locus_tag="WGO87_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0392408.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            5642..5950
                     /locus_tag="WGO87_001040"
     CDS             5642..5950
                     /locus_tag="WGO87_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392409.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(6229..6384)
                     /gene="rpmG"
                     /locus_tag="WGO87_001041"
     CDS             complement(6229..6384)
                     /gene="rpmG"
                     /locus_tag="WGO87_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0392410.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(6416..6649)
                     /gene="rpmB"
                     /locus_tag="WGO87_001042"
     CDS             complement(6416..6649)
                     /gene="rpmB"
                     /locus_tag="WGO87_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0392411.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(6899..8425)
                     /gene="farB"
                     /locus_tag="WGO87_001043"
     CDS             complement(6899..8425)
                     /gene="farB"
                     /locus_tag="WGO87_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0392412.1"
                     /translation="MDYPPLKGAALAWITLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVIFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(8449..9621)
                     /gene="farA"
                     /locus_tag="WGO87_001044"
     CDS             complement(8449..9621)
                     /gene="farA"
                     /locus_tag="WGO87_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0392413.1"
                     /translation="MATRGDEVKIQNGQEADSAKAKRKRRLTALTLLFALAAVAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            complement(9665..10024)
                     /locus_tag="WGO87_001045"
     CDS             complement(9665..10024)
                     /locus_tag="WGO87_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0392414.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     regulatory      10239..10283
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            10288..10761
                     /gene="queF"
                     /locus_tag="WGO87_001046"
     CDS             10288..10761
                     /gene="queF"
                     /locus_tag="WGO87_001046"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0392415.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFETLARKRLFEHDSQ"
     gene            10836..11522
                     /locus_tag="WGO87_001047"
     CDS             10836..11522
                     /locus_tag="WGO87_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0392416.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT         3118 a         2857 c         2825 g         2880 t
ORIGIN      
        1 agaaaattta tccaatggat tgaccgtgaa gaaaataagg tcgtctgaag agtctgatat
       61 gtcaggctat acaggcggcc tcgttgtttc aggtggcata tcattaattg acagacttga
      121 tattatggaa aatgtaaacc gcatcgttgc agaaggcatt gccgcagtag aagctgcgca
      181 agacttcaac gctctagaac aaatcaaagc ccgttatctt ggcaaaaccg gcgagttgac
      241 cggacttctg aaaactttgg ggcaaatgtc gcctgaagag cgcaaaacca taggtgcgca
      301 tatcaatgaa tgcaaaaacc ggtttcagac ggcttttaat gccaaacgcg atgccctcaa
      361 cgaagtcaag ctgcaagccc gacttgccgc cgaagccctc gatattaccc tgcccggacg
      421 cgctcaggaa ggcggcagcc tgcatcccgt aaccctgacc ttgcaacgtg tggtcgaact
      481 ctttcacgga atgggtttcg aagtggcgga cgggcctgaa atcgaagacg attttcacaa
      541 tttccaagcc ctgaacatcc ctgcaaacca tcctgcccgt gcgatgcagg atacgtttta
      601 cgttgaaaac ggcgatgttt tgcgtacgca cacttccccg attcaaatcc gctatatgct
      661 cgataaaaaa gagccgccca tccgcattat cgcccccggc cgcgtttacc gtgtggacag
      721 cgatgccacg cactcgccta tgttccatca ggcggaaggt ttgtgggtag aagagggcgt
      781 aacttttgcc gatttgaaag cagtgttcac ggattttatc cgtcgcttct ttgaacgcga
      841 tgatttgcaa gtacgtttcc gcccgtcttt cttcccgttc accgaaccgt ctgccgaaat
      901 cgacattatg ggcgaaaacg gcaaatggct ggaagtcggc ggctgcggca tggtgcatcc
      961 caacgtgttg aaaaatgtga atatcgattc tgaaaaatac accggtttcg ctttcggtat
     1021 cggtctcgac cgtttcgcga tgctgcgtta caacgtgaac gatttgcgct tgttttttga
     1081 taacgatttg aattttttga agcagtttgt aaaatgattg tgtaggctgc ctaaaaatga
     1141 aaaaagcagc ctgtgcctac ctcagaaagc taaatcatga aacgctatgt cgctccatta
     1201 tccgcctgct atgcgtttac tatggtgctg tggttgctgt ctttttacaa tgtggcttgt
     1261 acaaatattg ttgatatata ttagggggat tatatgatta aatcatttga gcttgaaaaa
     1321 ttcggaccga ttagcttatt gaaggctgat aatttaggga aaataaattt aattattgca
     1381 gaaaatagca aagggaaaac ttttatttta aaggcattat atagtgtttt aaaaagtcat
     1441 gaggaagctc ataaaggaaa gaatattcgt gatttttctg aggaattgcg agataagcta
     1501 tattggacgt tccaagtaga ggaaattggt gatttagtta caagaggaaa agaaaatcca
     1561 aacgaacggc ctcttaagtt aagtatgact ttagaagata gttcttcagt tttgttttct
     1621 tttggtagaa ctaccaaaaa gctaattaaa ccggagctat acgaactttc tcctagaata
     1681 aacgctaact ctattttttt accacccaaa gaagttctat cactatttga tgtaattaaa
     1741 aaatcagagg aagaaaagag atttggtttt gatgctactt acattgattt ggtaaaagca
     1801 ttggatatta aaccaacaaa agggaggaat taccctgagg cagcggaagc tagaaaggat
     1861 ctagaagcat tgtttggcgg ttatgttagc tacaatgata aaaagaaggc atgggtatat
     1921 gaaaaagata gacaaacttt ctctatcaat tctacagcag agggggtaaa aaagattggg
     1981 atattagaca ctctattagg aaatcggttt ttgagtcctg gatcaatctt atttattgat
     2041 gagccagaag ctaatctaca tccgaaagca attaatcaat ttctagaaat tctgttttcc
     2101 ttatctaaat ccaaattggg aatacagatt tttatgagta cgcattcata ttttgttatc
     2161 aagaagctgt gtttattagc tagaaagcat agtgaaagta ttcctgtttt tatgcctggc
     2221 aaagaaaagg aacaggctac ttgggtgcag gaaaatttac aaaatagcat gccggataat
     2281 gaaattattg gtgagtctat tcgattgttc agagaagaaa taggtgtttt atctccggaa
     2341 acagcatgga ttggttaggt ttttatgagt gaaataataa atctcactga atcaggtata
     2401 acttttaatc tagtaaaaaa taacctgttt tatatagaac catatatccg gaagagaagg
     2461 caagaaggta ttaagacagt agagtttatc tatctgcaag aagataattt gttatttgtt
     2521 gaggcaaaac aaagtattcc aaatcaagaa agatctcctg agcgttttga tgaatatatt
     2581 tcagagattt atcaaaaatg gtgtaatgca ttaaatgttg aaattttggg aatattaggc
     2641 agagaagata tcaaagaaac aattatgcct agtgcttttt caaatttgca atggggtagc
     2701 atagaaatca aattattact ggttatcccg gatgtgctgc ttaattatct tgggcaattg
     2761 aatgaattaa taaagcaaga atttaataag aaagatactt tgcggttgat ttctttatgg
     2821 aacatttctg tagaagtaat taatagggac ttagccattc aaaaaggttt agctagctct
     2881 tgaaaatcta aagttaatta gttaattgag aacataacat gcaattctcc tactcatggc
     2941 tgaaaaccca agccgatacc gaactttcct ccgataagct ggaacatctg ttaaccatgt
     3001 ccggcttgga agtggaagaa gccgagactg ccgcgcctgc gtttgcgggc gtggttgttg
     3061 ccgaagtgaa atccgttgaa aaacatccgg atgcagaccg tttgaacgtt acccaagtcg
     3121 atgcgggtac gggtgggttg gttcagatcg tgtgcggtgc gccgaatgtg aaagcgggca
     3181 tcaaagtgcc gtgttcgctg ccgggtgccg ttttgccggg taatttcaaa atcaagccga
     3241 ccaaaatgcg cggcgtggtg tcggacggga tgttgtgttc caccgacgaa ctcggtctgc
     3301 ccgacgacgg tgtgaacggc ctgcacattc tgcctgaaga tgcgcccgtc ggtaccaata
     3361 tccgcgaata cttggatttg gacgatacgc tgtttacgtt gaaaattacg cctaaccgcg
     3421 ccgactgctt gagcatcaaa ggcattgcgc gcgaagtgtc cgcattgacg gggtgcgcgt
     3481 tcaggcagcc cgaaatccat accgcgccga tcacgggcag tcgaaaacag cccgtgcaga
     3541 ttaacgcgcc tgccgattgc ggccgtttta tcagccgcgt tattgaaaat gtcaatgcga
     3601 aagcggctac tcccgattgg atgaaacaac gtttggagcg cagcggcatc cgcagtattt
     3661 ccgcgctggt ggacatcggc aattatgtga tgctggaaat cggtcagccg atgcacgttt
     3721 ttgatgccga caaactttcc ggcagcctgc acatccgccg cgcgcgcgaa ggggaaacgc
     3781 tggaatgcct gaacgagaaa accgtttccc tgtctgaaaa cacgctggtc gtggcggacg
     3841 aaaaaggcgt gttgagtttg gcgggcttaa tgggcggcgc ggcgagcgcg gtttcagacg
     3901 gcacgcaaaa tatcgtgctg gaagcggctt ggtttgcgcc cgaaatcatc gccggcaaat
     3961 cgcgccaata cggtttcggt tcggattcgt cgttccgctt cgagcgcggc gtggattacc
     4021 gtttgcaggc ggatgccatt gagcgtgcta ccgaattggt gttgcagatt tgcggcggtg
     4081 cggcaggcga aatggtggaa gcgcaaggcg aattgcccga agcgaagcag gtcggattgc
     4141 gtttgggccg tctgaaaacc gtgttgggcg tggacattcc tgccgaacag gtggaaacca
     4201 ttttgcaaca cttgggcctg cagcccgaga aaacggcgga aggcttccgc gttaccgcgc
     4261 cgagcttccg ttttgacatc gaaattgagg ctgatttgat tgaagaaatc ggacgcgttt
     4321 acggctatga aaacatcccc gacgattaca cgtcaggccg tctgaaaatg ctggaactgc
     4381 ccgaaacacg ccgcccgcgt tttgccgttt acaacgaaat ggcggctcgc ggttaccgcg
     4441 aagtggtcag ctatgccttc gttgacgagc agtgggaaca agattttgcc gccaacgccg
     4501 accccatccg cctgcaaaac ccgctggcgg cgcagtatgc cgtgatgcgt tccacgctca
     4561 tcggcggctt ggtggaaatt ctgcaaaaca atctgaaccg caaacaaaac cgcgtgtgcg
     4621 tgtttgaaat cgcccgcgtg ttcagcaaag gttcagacgg ccagtttgtc caaaacgaac
     4681 gcatcggcgg attgtggtac ggcgcggtca tgccggaaca atggggcggg aaaacgcgca
     4741 atgcggattt ttacgacatc aaggcagatg tggaaaatct gttgaaaaac aaagcagtcg
     4801 agttcgttaa aaccggacat cctgccctgc atcccggacg cgccgccaat atcgtttcag
     4861 acggcaaagt catcggcttt gtcggcgaac tgcatccgaa atggctgcaa aaatacgacc
     4921 tgccgcaagc gccgctggta tttgaaatcg atatggcggc cgtgttggaa tgcggggaaa
     4981 cgcgctatcg ggccgtatcg aaattccagc cggtgcgccg cgatttggcg tttgtgatgc
     5041 cggaagctat gagccatgat gatttgctgc ttgtcttgaa aggcgcggca aacaagttgg
     5101 tacaggaaat cagcgtgttt gacgtgtatc gcggcacggg actgcccgaa gggatgaaga
     5161 gcgtggcggt caaagtgatt ttgcaggata tggaaaacac gctgacggat gaggcagtcg
     5221 agccgcttat cggaaaactg attggcgcgg caacggcggc aggggcgcgg cttcgcagtt
     5281 aaaaaataaa tattcgcttg aattttaaat aaaaattggt aataatccac aactgttaca
     5341 acagaaggta atcatatgac tctcactaaa gcagaactgg ccgatatttt ggtagacaaa
     5401 gtcagcaacg tcaccaaaaa cgatgccaaa gaaatcgtcg aactcttttt tgaagaaatc
     5461 cgcagcactt tggcaagcgg cgaagaaatc aaaatttccg gtttcggaaa tttccagttg
     5521 cgcgacaagc cgcaacgccc gggtcgtaat cctaaaaccg gcgaggaagt gccgattacc
     5581 gcccgccgcg tggtaacttt ccatgccagc cagaaactca aaagcatggt ggaacactac
     5641 tatgacaaac aacgttgatt tgaaggttcc cgcaaaacgc tatttcacgc tggacgagtt
     5701 gtgcggactg ttgcaaatca gcccctatgg ttttgcgcaa tggcagcatg atcacggtgt
     5761 ggttgtcggt tacggcggcg aacgctacac ccgtttggat gtggtgaaac tgttgaaatt
     5821 gcagagcacg tttgcaccgt atgcagaagg tgcggaatcg ggttcggacg gcaaccgtcc
     5881 ggttacgctt caggaaatcg gagacggtct gaaagacctg ttggcggatt tggataagga
     5941 attgtgctga tttgaggccg gttgcaggta tgcagccggt tttgttttac acgctaaaaa
     6001 ataattatag tggattaaca aaaatcagga caaggcgacg aagctgcaga cagtacaaat
     6061 agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc tatccttggc
     6121 agacggtttg cagcagattc gcatacggca gatgtttcaa gcggaaaaga aacacgataa
     6181 aaataaaaaa gcctccgaac agtcggaggc tttgctttca aactgaaatt atttcagttt
     6241 ggtttctttg tacactacgt gtttgcgggc aaccggatcg aattttttaa tttccagttt
     6301 gccgggcata gtgcgtttgt ttttggtagt ggtgtagaag tgaccagtac ctgcactgga
     6361 ttccagtttg attttatcgc gcattgcagt aatccttaat taaatagtgt ttaaattaag
     6421 cttcgccgcg agcacgcaaa tcagccaata cgacatcaat gcctactttg tcgatggtac
     6481 gcagtgcagc gttggaaacg cgcaggcgaa cccagcggtt ttcactttct acccaaaaac
     6541 gacgtgattg caagttgggc aaaaaacggc gtttggtttt gttgttggcg tgcgatacgt
     6601 tgttgccgga catcgggcgt ttgccggtca ctttgcaaac tcgtgccata atcagtctcc
     6661 aaacttctaa aatagtaaaa cgcgatttat accacgaaaa acagtgttag ttcaagtgtt
     6721 ttaaagaaaa acggctgttt tgcgcggata atccttccga ttgtcgcatc tgtggaacgt
     6781 ttgcaggtgc gaaacagatg gggcgcgatt atagcggatt tcgggaactg ctgcattcaa
     6841 aatgccgtcc gaacatattt ccagtatttt cagacggcat ttcaagtttt caaatccctc
     6901 aatgtccacc gccgccgccg ttgtggaacg gcggttttgc cagccatatg acgggtatca
     6961 tgataatgaa taacaagctg cccgccataa agatttcgtt cgagccgata ataaaaccct
     7021 gctgggtaat ggtattgttg atgatgccta gggtttgaat gtcggaaacg ccgtgctggg
     7081 acaaatgagc ggccgtttcg tgcaatgttg cggaataggg cgtgatgtgt tcggcaaagc
     7141 gtgtatggtg caacgcttcg cgccgttccc acagggtgct gacgacggat acgccgacac
     7201 cgcccatcag cacgcgcaag aaattcgaca ggctgcctgc ggcggcaatc tgtccgccct
     7261 tcatgtgcga cagtgtgatg gtggtcagcg gcaggaagaa catggcaacg cctgccccct
     7321 gccaaaactg cggccagatg acgttgccga tatccatgtc ggcataaaaa tcggtgcgcc
     7381 aatagaaagt aaaggcaaag gtcaggaagc tggcggttac gagcaggcgc atatcgattt
     7441 tattgccgaa cctgccgatt aacggagaca ggaagacggg caggatgccg acgggtgcgg
     7501 cggcaagccc cgcccacgtg gaggtatagc ccaggttggt ctgcaacact aacggcagca
     7561 aggtcagcgt ccccatatac accataaaac ccaatgatgt ggcaattacg ccgacggtaa
     7621 aattccgatc tttaaacagc gataaatcga caatcggata tttttctccc aattcccaaa
     7681 caataaaata cgacaaacac accagtgcga ctacgcccaa ggtaatgatt tctccagagg
     7741 cgaaccagtc gagttcctta cccctgtcca gcatcatctg taacgcgccg ataccgacta
     7801 ccatcaatgt aagcccgaca tagtcggtcg gcattttaac ggtttccgtt tcccgatatt
     7861 tcaaatgttt ccatgtaatc catgccgata tgataccgat agggatatta atgaagaaaa
     7921 tccaacccca atgccagttt ccggaaatcc aaccgccgag tatcggcccg agaacagggg
     7981 cgacaacgac ggtcattgcc cacaatgcca gtgccagcgt ccgttttgcg ggcggatagg
     8041 atgccattaa caggctttgc gacaggggaa tcagcggccc ggcgataaag ccctgcaaga
     8101 tgcggaaaat taccaaagcc tgaaggttgg gcgcgatgcc gcacagccac gatgtgatga
     8161 cgaaaccgac agcggcggcg gtaaacaatt tgacctcgcc gatacgtttt gccaaaaagc
     8221 ccgttaacgg cacggaaacg gcgtttgcca cagcaaaaga agtaatgacc cacgtcccct
     8281 gagtggttgc cgcaccgagg ttgccggcga taacgggaac ggcgacattg gcgatagtcg
     8341 tatctaaaac ttccataaat acggcaagcc ccaaagacag cgtaatccat gccaatgccg
     8401 cacccttaag cggtggataa tccattgtgt cctccgtttc agacggcatc agggcgcgga
     8461 ttgcccgagg atttcatcga ccgtccgatc gacttccgac cagtcggtac tttccatttc
     8521 cggcaatgcc gcacccggcg tttttgaaac aggcgcgcct gcggcggaag tatccacttt
     8581 aaccgtcatc gacaaaccga tacgcaacgg atgcctgtcc acatcttcgc ggttcagcac
     8641 gatacggacg gggacgcgct gcaccacttt aatccagttg cccgttgcgt tttgcgccgg
     8701 aatcagcgaa aacgcgctgc ccgtacctgc cgaaaaacct gccaccctgc cgcgataaac
     8761 aatttgtttg ccgtacaaat cggacaccag ctcggcaggc tgtccgattt tcatatgccg
     8821 caactgcgtc tctttaaaat tggcatccac ccacacatcc gacagcggca ccaccgccat
     8881 cagcggcgcg cctgccgcca cctgctgccc gacctgcacc gaacgcttcg ccacctgacc
     8941 gtccgccggc gcgcggattt gcgtccgctg aaggttcaac cacgcatctt tcaacctgcc
     9001 gattgccgtc tgaaccgccg gctgttcgcg caaagaaacc tgaccgccca aagccgcgcg
     9061 tgccgaagac tcttccgcca aagccgcctt aactgccgcc tgcgcctgag acactgccgc
     9121 acgggcgtgt gccagctctt cggcggacac cgcgcccgat tccgccaaag cagaccggcg
     9181 gcgcaaatca tcctgcgcgc gtgccaaatc cgcacggcgc aaggcaacct gcgcccccgc
     9241 ctgcgaagtg gcggcatttt gccggcggtt ttgccgcacc gcctgaacca gctcgttttt
     9301 tgcccgctcg taagccagca catcattatc gtcgtccaat accgccagca cgtcgccttt
     9361 tttcacggca tccgtatcgt cgtgcaaaac cttccgtacc gtaccgccct tttgcggcgt
     9421 aacctgaacc acgcgtccgg caacataagc gtcttccgtt tcctcttcgt gctgccacca
     9481 taaaaaaaac gccgacccag cggctacggc ggcaagcgcg aacagcagcg tcaatgccgt
     9541 caggcggcgt ttgcgtttgg cttttgcgct atcggcttct tgcccgtttt gaattttcac
     9601 ttcgtctccg cgtgttgcca ttattccaat tccgaatact tctaatacta tgtttttgat
     9661 ttaattagca ctgataatct tcaaattccc acgaaaaaaa acgaagtaaa taagtcaatg
     9721 acttttccca agtttctttt gaacattctt taagaatttt ctcaatttcc gatttaataa
     9781 cagaatgatt aaattcattc ataatcatca tacccgctcc ccatttaacc ctttgatttt
     9841 ggaaacaatt atgcaaaatc catttaggag agcatatgcg aacagaaaat atatctgcag
     9901 catcactatc atcagttcct atgtctaaat caattcccac acaaaaattg tctttgattt
     9961 cgggaacgaa atcttcaaag gcacaatcgt aaagattgat ggctttcaat tctaggttaa
    10021 tcattttata ttcaaaataa ataaaaccaa ttaaagattg gttaggattc tataccctgg
    10081 caataactca atcaagttaa tgatttagaa tgaatattca aataaactgt atcagcccgg
    10141 cctcacagaa ctatttatat ccacacatca ccgagtaaag caattaccca tactaaccga
    10201 agaaaactac acaaataccg ccacttatat tacaatcgcc gctcgtggtt cgaaaacctc
    10261 ccacattaaa aaactaagga aaccctcatg tcccgcaaca acgaagagct gcaaggcatc
    10321 tctcttttgg gcaatcaaaa aacccaatat ccgaccggct acgcgcctga aattctcgaa
    10381 gcattcgaca acaaacatcc cgacaacgac tatttcgtca aattcgtctg tcccgagttc
    10441 accagcctct gccccatgac cgggcagccc gacttcgcca ccatctacat ccgctacatt
    10501 ccgcacatca aaatggtgga aagcaaatcc ctgaaactct acctcttcag cttccgcaac
    10561 cacggcgatt ttcatgaaga ctgcgtcaac atcatcatga aagacctcat tgccctgatg
    10621 gatccgaaat acatcgaagt attcggcgag ttcacaccgc gcggcggcat cgccattcat
    10681 cctttcgcca attacggcaa agcaggcacc gagttcgaaa cattggcgcg taaacgcttg
    10741 ttcgaacacg acagccaata aaccatcctt cccatatttc agacggcatt ccttcgatac
    10801 gatatgccgt ctgaacgcct ttccgaaaga tttttatgta cgcatttact gccgcacagc
    10861 aacagaaggc actcttctgg ctggtgcttt tccatatcct catcatcgcc gccagcaact
    10921 atctggtgca gttccccttc caaattttcg gcatccacac cacttggggt gcgttttcct
    10981 tccccttcat cttcctcgcc accgacctga ccgtccgcat tttcggttcg cacttggcac
    11041 ggcggattat cttttgggtg atgttccccg cccttttgct ttcctacgtc ttttccgttt
    11101 tgttccacaa cggcagttgg acaggcttgg gcgcactatc cgaattcaac acctttgtcg
    11161 gacgcatcgc cttggccagc tttgccgcct acgcgctcgg acaaatcctt gatatttttg
    11221 tattcaacaa attacgccgt ctgaaagcgt ggtggattgc cccaaccgca tcaaccgtca
    11281 tcggcaacgc cttggatacg ttggtatttt tcgccgttgc cttctatgca agcagcgatg
    11341 aatttatggc ggcaaactgg cagggcatcg cttttgtcga ttacctgttc aaactcaccg
    11401 tctgcggtct gtttttcctg cccgcctacg gcgtgattct gaatctgctg acgaaaaaac
    11461 tgacgaccct gcaaaccaaa caggcgcaag accgccccgc gccctcgctg caaaatccgt
    11521 aaaacagccc tgccgggaat cgggacggtt gggttttctt ttatttgcaa acactgtcgg
    11581 aacggataaa tccggattcc tacctacttc gtcattcccg cgaaagtggg aatccggtgc
    11641 gttgggtttc catcatttcc gataaactcc tgccgcgttg
//
LOCUS       DAWFBO010000056        11611 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFBO010000056 DAWFBO010000000
VERSION     DAWFBO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.056"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(174..866)
                     /locus_tag="WGO87_001048"
     CDS             complement(174..866)
                     /locus_tag="WGO87_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0392417.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGAPKYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(974..2167)
                     /locus_tag="WGO87_001049"
     CDS             complement(974..2167)
                     /locus_tag="WGO87_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0392418.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2296..3033)
                     /gene="pxpA"
                     /locus_tag="WGO87_001050"
     CDS             complement(2296..3033)
                     /gene="pxpA"
                     /locus_tag="WGO87_001050"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0392419.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3020..3949)
                     /locus_tag="WGO87_001051"
     CDS             complement(3020..3949)
                     /locus_tag="WGO87_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0392420.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3946..4593)
                     /gene="pxpB"
                     /locus_tag="WGO87_001052"
     CDS             complement(3946..4593)
                     /gene="pxpB"
                     /locus_tag="WGO87_001052"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0392421.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4777..6984
                     /gene="uvrD"
                     /locus_tag="WGO87_001053"
     CDS             4777..6984
                     /gene="uvrD"
                     /locus_tag="WGO87_001053"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0392422.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQMPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNATLESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7341..7880
                     /locus_tag="WGO87_001054"
     CDS             7341..7880
                     /locus_tag="WGO87_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0392423.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8070..8285)
                     /locus_tag="WGO87_001055"
     CDS             complement(8070..8285)
                     /locus_tag="WGO87_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392424.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8293..8496)
                     /locus_tag="WGO87_001056"
     CDS             complement(8293..8496)
                     /locus_tag="WGO87_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392425.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8409..8768)
                     /locus_tag="WGO87_001057"
     CDS             complement(8409..8768)
                     /locus_tag="WGO87_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0392426.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8821..9198
                     /locus_tag="WGO87_001058"
     CDS             8821..9198
                     /locus_tag="WGO87_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0392427.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            9397..10017
                     /locus_tag="WGO87_001059"
     CDS             9397..10017
                     /locus_tag="WGO87_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0392428.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            10040..11491
                     /locus_tag="WGO87_001060"
     CDS             10040..11491
                     /locus_tag="WGO87_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0392429.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSIAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT         2852 a         3076 c         2836 g         2847 t
ORIGIN      
        1 ctctttcagt tacagcagct tgatcccttt cccttatcca acgggggaag gctaggatag
       61 ggtggcttgc aaatatacag aacaagggac aagagccacc ctctctccaa ccctctccct
      121 ccgtacggga gggggtggat tctcgcgggc gaagcccacg ctacggttag cctttacccc
      181 agcacaaaca attcccgccc gtgcgccttc agccaacttt tagcattgtc ggtatgcggc
      241 gtcagcgtgt tcaccaaatg ccaaaagcgc ggactgtggt cggggtggcg gaggtggcag
      301 agttcgtgga tgcagacata gtcggcaacg tacttgggcg cgccgatcag ccgccagttg
      361 aggcggatgc cggtgtgcgg gcggcatacg ccccaaaagg ttttggcgtt gctcaggtct
      421 gtggcggtgg gcgtcagtcc tgtttcggct gcgtgttttt caaggcgggg cagcaggtat
      481 tcgcgggcgc gttcgttcaa caggcggcgc aggtggtcga tttgtgcggc ggtttctttt
      541 cggggaagca ggatttcaga cgacgtgata cggatatggc tttggctgtg ggtatccagc
      601 ttggtcttta ttccccgata ccaaatccac tcgggtaagt ttgggtggga aacaggatgc
      661 acgggcgttt tggcaagcgt gttccgcaaa atcgtttcgt ttgccgccag ccagtttgct
      721 aacgcgtggt cttgaaaaaa gggtgggacg ttgatgctga ccgtctgcat attgacgggg
      781 cgcagaatca gatttttctt ggcactgcgt ttgagttcga tttcgatgca caaaccgtcg
      841 gaaagagtat aggtgaagcg tttcatagtt gtgaataggt ttcagaccgg atacatcgtc
      901 tgaaacagga attttccata tcaggcggca aacttcggat aatatacaaa atcaaacatc
      961 tgcgctacaa ggttcagccg aacaagccgc cgatatattt gctgatggta atggcgctga
     1021 gtactgccat caaaccgacc acaatcacgc cggaaacggt gagccacagc gggtgtttgt
     1081 agtcgccgac aattttggtt ttgtaggcgg caatcagaat cagaccgagg gaaatcggta
     1141 aaatcaggcc gtttaatgcg cctacgaaca ccagcacctg cgccggtttg ccgatggtgg
     1201 aaaatacggc ggtggacacg gcgataaagg caataatcca tttgttttta ttgcgttcga
     1261 tagacgggct gagaccggag aagaacgaca ccgaagtata agccgcacca atcaccgaag
     1321 taatcgaagc cgcccaaatc accacgccga aaatcagcag gccgatgtat cccgccgcat
     1381 attcaaacgg tgtggaagca gggttgtcgg gattgagctg tacgccttgg ctgaccacgc
     1441 ccaaaaccgc caaaaacaat acaatccgca taatcgaggc aatcaggatc gcccgcaccg
     1501 agctttggct cacttccggc aacgccgatt tgcctttgat gcctgcgtcc agcagacggt
     1561 gcgcaccggc gaaggtgatg tagccgccga ccgtgccgcc caccagtgta acaatcgcca
     1621 ttgcatcgag tttttccggc ataaaggtat gcacggcggc atctgccagc ggcggattcg
     1681 cctgccatgc cacataaacc gtcagcgcaa tcattacgaa acccatcact tgggcgaatt
     1741 tgtccatcac tttgcctgct tctttaaaca gaaacacacc gatggcaatc acgccgctga
     1801 tcacggcacc ggtttccggc gacagtccgg tcagcaggtt cagacccaag cctgcgccgc
     1861 cgacgttgcc aatattgaac gccaaaccgc ccatcacaat cagcacagcc aagaaatagc
     1921 ctgcgccggg caagacctga ttggcaatat cctgcgcctg tttttcggaa acggcgacaa
     1981 tccgccaaat attgagctgc gccccgatgt cgagcagaat cgagagcaga atcacaaagc
     2041 cgaaacttgc cgccagtgct tgggtgaagg tggcggtttg ggtcagaaag cccgggccga
     2101 tggcggaagt cgccatcagg aatgcagcgc cgattaaggc atttctgcgg tttttttgat
     2161 tagacataat cgcttatcct ctataaaatt ggttgttgct gtgtttgggc gaaacctgcg
     2221 gttttagcta cgcagaaact cgctttgctc gttttggcga aacctgcggt tttcagacgg
     2281 cctatgaact gtttttcaag cagaaacttt gatgcctgcc gccagtagtt cctgccggat
     2341 tttttcggca aacaccacgg cgtgcggccc gtctccgtgc agacagatgc tgtcggcttg
     2401 cacggcaacc aggctgccgt tcactgcttt gacccgcccg tcccgcacca tctgcaatac
     2461 ttgggcgatg gcttcttcgt cgctgtccac ctgcgcatcg gggcggctgc ggggaaccag
     2521 cgtaccgtcg ggcatatagc ggcggtcggc gaatacttcg gaaatcacac ccaagcctgc
     2581 ggcttttccg gcttccaaga gcaggctgcc ggaaagtgcc atcaatttca atttcgggtc
     2641 gaaatccgcc acaattcggg caacggtatc cgccagcgca cggtttttcg ccgcttggtt
     2701 gtacattgcg ccgtgcggtt tgacataagc catttccaaa ccctgatcac ggcacaaggc
     2761 ctgcaatgcg cccaactggt aattcagaca cgcccgcaaa tcggcttcgg acagattcat
     2821 ttcggtacgg ccgaagtttt cccgatcggg atagccgggg tgtgctccga tgcgcacgcc
     2881 gttttgttgg gcatacgcca atgccgcccg aatatcggca atgctgccgg cgtgttgggc
     2941 gcaggcgatg ttggccgaag taatcagctg caacaaggct tcgtcgctgc cgcagccttc
     3001 ggcgagatcg gcgtttaaat caacctgctt catgggtgat tctccgtatt tggttcagat
     3061 aggcttggtt tttgcgccgc agggcggtgg cttctttcaa gccgattatt ttgaatttga
     3121 ctttgctgcc gaagcgcacc tgtgccagcc tgcccaaatc ggcggcggca acggtagcga
     3181 ttttcggata accgccggtg gtttgtgcat cggccagcag gataatcggt ttgccgccgg
     3241 gcggcacctg cacggttcct gcctgaacag cgtgggacag catttccaaa ggttgcgaca
     3301 gggtcagcgg ctgtccgtcg aagcggtagc ccatgcggtt gctatcgctt tgcagcgtcc
     3361 acgtttcccg ttccagattc agacgccctt tttcactgaa agcggcatat tccgacgaag
     3421 gaacaaggtg gacggtatcg gtaaacggta tcggggcaat gccgactttg gacaattcct
     3481 gcgcaccttt gccgatgggg agacaatcac ctttttgcag cattctgccc tgatggccgc
     3541 cgaaaccggc tttcaggtcg gtgcttctcg aacccatcac ttccggcaca tcaaatccgc
     3601 ccgccacgca cacatagccg tacatgccct gcacggcacg caccattttc aaggtctgcc
     3661 ctttgcgggc ggtataacgc caatacgaat agaccggttc gccgtccaat tccgcctgat
     3721 acacggcacc ggtgagacaa aacggcgtat cccgttcaaa caccagcatt atcccgccca
     3781 aagcgatttc gattgcggcc gtgccttcgt cgttgcccaa taaaatatta cccgccgcca
     3841 aagcaaccgt gtccatcgca ccggcatgac cgatgccgta acgccggtgt ccgtagcgtc
     3901 cggtatcctg aatatgcgcc ggtgcctgca ctgccgaaac gtgaatcatg gctcaatcct
     3961 ttctgcaaca aagcggactt ggtcacccgc cgccagcagg gtcggcggat tcaaatcggc
     4021 tcggaacaag ggtaattcgg ttctgccgat aatctgccag ccgccgggcg aagcgaacgg
     4081 atacacaccg gtctgactgc cgccgatacc gaccgaaccg gcaggaacgg acgttctcgg
     4141 cacggcacgg cggggcgtgt gcaatgcttc gggcaagccg cccagataag ggaaaccggg
     4201 ctggaagccc atcataaata cggtataagt ttgcgccgta tggcggcgga cgatttcgga
     4261 aataaccgtc tgatggaaag cagcgacttc cgccaaatcc gggccgtatt cgccgccgta
     4321 gcagacggga atttccacca gtttgccctg atggtctgta acggcggtgt gttcccacac
     4381 atattgcaat tcatcggcaa gcgtcgccaa atcggtatcg aaacgggtga acacggtcag
     4441 attgttcatg ccgaccacca cttcctcaat cctgtcgtgc tgcccgagcg cagcggcaaa
     4501 cgcccacaac ttttgctgtt tgcccagttc ggaaggcgca ttcagtcggc agaccaaagc
     4561 ggattcgctg attggtgtga tctctattct catttgttgt tcattttggt tatgttttaa
     4621 tgaatctata tgcaggggcg gcggtttgtc aatatcttct gtgctgcatc atcaaaccgt
     4681 cgattggaaa agtgctgccc tgccgctgca ctttttcaga cgaccttaaa ccgtttctat
     4741 taaaatagcg cattccactt ttcagacggc atccttatgt ttcccgacca atccgccccc
     4801 aacctgctgc aaggcttgaa tcccgaacaa ctctccgccg taacctggcc gccgcaatcc
     4861 gcccttgtgc tggcgggcgc gggcagcggc aaaacgcgcg tgctgaccac gcgcatcgca
     4921 tggctgttgc aaaccggtca ggcaagcgtg cacagcatta tggcggtaac gtttaccaac
     4981 aaagccgcca aagaaatgca aacccgactc ggcgcgatga ttcccatcaa cgtccgcgcc
     5041 atgtggctcg gcacgttcca cggtctctgc caccgctttt tgcgcctgca ccaccgcgac
     5101 gccggtctgc cgtcttcctt tcaaatcctc gacggcggcg accagctttc cctcatcaaa
     5161 cgcctgctca aaagcctcaa catcgccgaa gaaatcatcg cgccgcgttc gctgcaaggc
     5221 tttatcaacg cgcaaaaaga atccggcttg cgcgcttccg tcttgagcgc gcccgacccg
     5281 cacacaagcc gcatgatcga atgctacgcc gaatacgaca aaatctgcca acgcgaaggc
     5341 gtggtcgatt ttgccgaact catgctccgc agctacgaaa tgctgcaaag caacgaaatc
     5401 ctgcgccaac actaccaaaa ccgcttcaat cacattctgg tcgacgaatt ccaagacacc
     5461 aacaaactgc aatacgcctg gctcaaactc atggcgggcg gcaacgcggc ggtatttgcc
     5521 gtcggcgacg acgaccaaag catctaccgt ttccgtggcg caaacgttgg caatatgacc
     5581 gcgctgatgg aagaattcca catcgacgcg cccgtcaaac tcgaacaaaa ctaccgctcc
     5641 gtcggcaaca tccttgccgc cgccaatgcc gttatcgaaa acaacgacga acgactcggc
     5701 aaaaacctgc gcaccgacgc cgaagcaggc gacaaaattc gctactactc cgcctttacc
     5761 gacctcgaag aagcccggtt catcgtggac gaaaccaaag ccctcgaacg cgaaggctgg
     5821 gatttggacg aaatcgccgt cctctaccgt agcaacgccc aatcccgcgt tatcgaacaa
     5881 agcctgttcc gcagcggcat cccctacaaa atctacggcg gcttgcgctt ttacgaacgc
     5941 caagaaatca aacacgccct cgcctacctg cgcctcgccg tcaatcccga cgacgacaac
     6001 gccctcttgc gtgtcatcaa cttcccaccg cgcggcatcg gtgcacgtac cgtcgaaaat
     6061 cttcagacgg cctcaaacga acaaggcatc accctctggc aagccgcctg caacgccggc
     6121 gcgaaagccg ccaaagtcgt cgccttcgtc cgcctgattg aagccctgcg caaccaagtc
     6181 ggacaaatgc ccctgtccga aatcatcgtc ggcatcctca aagacagtgg cttgaccgaa
     6241 cactaccgca cccaaaaagg cgacaaccaa gaccgcctcg acaaccttga cgaactcgtc
     6301 aacgccgcca tcgagttcaa accagaagac agcaactttg aaaccctgcc tgaaaacatt
     6361 tcagacgatc ccgccttccc catcctcgct ttcttgagca acgccaccct cgaatccggc
     6421 gaaaatcaag caggtgcagg cgaaaaagcc gtccaactca tgaccgtcca cgccgccaaa
     6481 ggcttggaat ttaacgccgt cttcctcacc ggcatggaag aaggccgctt ccccagcgaa
     6541 atgagtctcg ccgaacgcgg cggcctcgaa gaagaacgcc gcctcatgta cgtcgccatc
     6601 acccgcgccc gcaaacgcct ctacatcacc atggcgcaac aacgcatgct gcacggacaa
     6661 acccaattcg gcatcgtctc ccgcttcgtc gaagagatcc cacccgaagt attgcactac
     6721 ctgtccgtca aaaagaccgc ctacgacagc tacggcagcc cgcgccaaac cgccgcatcc
     6781 aaagacaaaa tcatcgacga ctacaaacag ccccaaacct acgcaggctt ccgtatcgga
     6841 caaaacgtcc gccacgccaa attcggcacc ggcgtgatta tcgatgccgc agataaaggc
     6901 gaatccgccc gactgaccat caacttcggc aaacagggcg tgaaagagtt ggataccaag
     6961 tttgcgaaat tggaagcgat gtaaatttga aatgcaggct ggatactcgt atccgacaaa
     7021 aatatttgac gcgtctatcg tttccgaaaa accgctgttg gaaaatgtcg gattcgagaa
     7081 tccgacctat ggcaaaaaac tgtagcaagg acaaggcaaa aggccgtctg aatccggtaa
     7141 caacaaattt tgccgaaacc tccgccgtca ttcccgcgca ggcgggaatc cagacctttc
     7201 ggcacgggaa cttatcggat aaaaggtttc tttagattcc acgttctaga ttcccgcctg
     7261 cgcgggaatg acggacggta aaagccgggt atagatacct aacccttgtt tatagctgaa
     7321 aatcaataag gaagaacatt atgtcccaag tttttaaaga ttttgatttg tcctccgtat
     7381 gggaatccga cagttgggca gatgaaaact acaaagaagc cccgtttacc cctgaaattt
     7441 tggctgccgt agaaagtgaa ctgggctata aattgccgca aagttttatt gaattgatgg
     7501 cggtacaaaa cggcggaata tttgtcaaaa actgttttcc gaccacacag agaaattcgt
     7561 gggcgaaaga tcatgtgcaa atttgcgagg tatcgggcat cggctttgaa aaagaaggga
     7621 gtttgtgcgg cgaaacaggg caaaaacttt ggatggaaga atgggaatat ccgcccatcg
     7681 gcgtgtattt tgccaacgac ccgtcaggcg gtcatgccat gtttgccttg gactatcggg
     7741 agtgtggaaa agacggcgag ccgaaagtgg tgtttgtcga gcaagaatcg gattttgaaa
     7801 tcgtcgaact tgcccccgat tttgaaacct ttatccgcag cttgcggcat gaagatgagt
     7861 ttattgacga ggaaatataa aacggcggtt gaaaaaccgg aatcatcaag ggaaaacggg
     7921 cgaaataacg gataatcgct tgaatccgta aggaaaacgg tttggtggaa cgcgccatcc
     7981 aagacctttg caaagaaaaa ctgtctccga cagcattgac attattaaca gaacttatca
     8041 agtttggagc tatctcaaat ataattcggt tatccttttg tatccattaa atcatatgct
     8101 tcaattaatt gttgttctag ctcttgtacc aattttgaat tgcgaattcc tgacacaatc
     8161 tcaaattctt ctgcatctat gcaaacacct gcataaattt caataacaag ggaacgcaat
     8221 aattgaagct cttctcttgt taaagaaata ataatgtcat cacctgtgta attgattata
     8281 ttcataataa ttttattttt gtttgtcaaa gtaagttttg cctaaggttg gtctaaatgc
     8341 agttccacca tcttttgaat ttgggtctct gattacaatt gctccagact tatcatccca
     8401 aattgctctt atgtgtttgg attgtaatct tcgaattccc aagaaaaaaa tcgtaataag
     8461 tttgaaagtg tcaaatccca agtttctttt gagcaatatt ctaatatttt atcaatttca
     8521 cttttaataa tcttatgatc aaactgttct aatattaatg cattagacca aaaaaaacct
     8581 tctttattac aatgatggga aatccattta ggagaacaaa tgcaaagtga aaaaatagat
     8641 gagccttgtt ctccttcgat tccgatatcc aaatctatcc atctatggaa attatctgga
     8701 atttcggggg taaatttttc aaaatcaata tcatataaat ttatgctttt taaatccaat
     8761 ttaatcatta gggctgtcct agataaatag ggaaattcaa attaagttag aattatccct
     8821 atgagaaaaa gtcgtctaag ccggtataaa caaaataaac tcattgagct atttgtcgca
     8881 ggtgtaactg caagaacagc aacagagcct gacagcattg tttatacgga ttgttatcgt
     8941 agctatgatg tattagatgt gagcgaattt agccatttcc gtatcaatca cagcacacat
     9001 tttgctgaac gacaaaacca tatcaatgga attgagaatt tttggaaccg ggcaaaacgt
     9061 catttacgca agtttaacgg cattcccaaa gcgcattttg agctgtattt aaaggagtgc
     9121 gaatggcgtt ttaacaacag tgagataaaa gttcaaattt ccattttaaa acaattagta
     9181 aaatcgagtt tatcttagtt gtccaggaca gccccattat ttttataaca ccgtgaagcc
     9241 gcacagcagt ttgaacagtg atacgccgtt tgcgggctta cgagtttatt ttcccggcct
     9301 gcagtttgag caatacggtg atttcctacg gttaatacaa atgtttacac attgatacat
     9361 ttcatttata gttccgccta tttgaaaata gaaaatatga attcgaccgc aagtaaaacc
     9421 ctgaaaggat tgtcgctggt gtttttcgcc tctggattct gcgccctgat ttaccaggtc
     9481 agctggcaga ggcttctatt cagtcacata ggtatcgatt tgagttcgat tactgtcatt
     9541 atttctgtat ttatggtcgg cttgggtgta ggtgcgtatt tcggtggacg cattgctgac
     9601 cgttttcctt caagtatcat ccccctgttt tgcatcgctg aagtatccat cggtctgttc
     9661 ggtttggtaa gcaggggtct gatttccggc ttggggcatc ttttagttga ggctgatttg
     9721 cccatcatcg ctgctgccaa tttcctctta ttgctgcttc ctacctttat gatgggcgcg
     9781 accttgccct tgctgacctg tttttttaac cggaaaatac ataatgttgg cgagtctatc
     9841 ggtaccttat attttttcaa cactttgggt gcggcactcg gatcgcttgc cgccgccgaa
     9901 tttttctacg tcttttttac cctctcccaa accattgcgc tgacagcctg ctttaacctt
     9961 ctgattgctg cttcagtatg gctgcgttac agaaaggatg gatatagtga acactaaacc
    10021 gaatactagt ttgatttata tgctttcttt ccttagcggc ttattgagct tgggtataga
    10081 agtcttgtgg gtaaggatgt tttcgttcgc agcacagtcc gtgcctcagg cattttcatt
    10141 tactcttgcc tgttttctga ccggtatcgc cgtcggcgcg tattttggca aacggatttg
    10201 ccgcagccgc tttgttgata ttccctttat cgggcagtgc ttcttgtggg cgggtattgc
    10261 cgactttttg attttgggtg ctgcgtggtt gttgacgggt ttttccggct tcgtccacca
    10321 cgccggtatc ttcattaccc tgtctgccgt cgtcagaggg ttgattttcc cgctcgtaca
    10381 ccatgtgggt acggatggca acaaatccgg acgacaggtt tccaatgttt atttcgccaa
    10441 cgttgccggc agtgcattgg gtccggtcct tatcggcttt gtgatacttg atttcttgtc
    10501 cacccaacag atttacctgc tcatctgttt gatttctgct gctgtccctt tgttttgtac
    10561 actgttccaa aaaagtctcc gactgaatgc agtgtcgata gcagtttccc taatgttcgg
    10621 catcctcatg ttcctactgc cggattctgt ctttcaaaat attgctgacc gtccggatag
    10681 gctgattgaa aacaaacacg gcattgttgc ggtttaccat agagatggtg ataaggttgt
    10741 ttatggggcg aatgtatacg acggcgcata caataccgat gtattcaata gtgtcaacgg
    10801 catcgaacgt gcctatctgc taccctccct gaagtctggc atacgccgca ttttcgtcgt
    10861 tggattgagt acaggttcgt gggcgcgcgt cttgtctgcc attccggaaa tgcagtcgat
    10921 gattgttgcg gaaatcaatc cggcataccg tagccttatc gcggacgagc cgcaaatcgc
    10981 cccgcttttg caggacaaac gtgttgaaat tgtattggat gacggtagga aatggctgcg
    11041 tcgccatcct gatgaaaaat tcgacctgat tttgatgaat acgacttggt actggcgtgc
    11101 ctattccacc aacctgttga gtgcggaatt tttaaaacag gtgcaaagcc accttacccc
    11161 ggatggtatt gtaatgttta ataccacgca cagcccgcat gcttttgcta ccgccgtaca
    11221 cagtattccc tatgcatacc gctatgggca tatggtagtc ggctcggcaa ccccggtagt
    11281 tttccctaat aaagaactgc tcaagcaacg tctctcccgg ttgatttggc cggaaagcgg
    11341 caggcacgta tttgacagca gcaccgtgga tgctgcagca caaaaggttg tctctcgtat
    11401 gctgattcag atgacggaac cttcggctgg ggcggaagtc attaccgacg ataatatgat
    11461 tgtagaatac aaatacggca gagggattta accgtcttaa agggtttcag gcaacgcagg
    11521 ttttaggtaa cgtcctgcta gttcaaaaaa accgcatcac agcagtcggg acaaaatggt
    11581 ttaaacattt tgtcccgaat tcttattcct a
//
LOCUS       DAWFBO010000057        11553 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFBO010000057 DAWFBO010000000
VERSION     DAWFBO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.057"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..622)
                     /locus_tag="WGO87_001061"
     CDS             complement(<1..622)
                     /locus_tag="WGO87_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEZ0392430.1"
                     /translation="MSFSIALPLVYLFIGILIGKTPFRKIKGTFSVILSRWIIPFVIV
                     YNITTYRDGMQYVMLWMIISMGLIILIGKRVDKDPVWNLCLCYLNIGWLGLPIVATLF
                     GDEAAQVLISAYVGSSLFGNSVGVGLLLNNSNFISGLKKTLKSPPVVALVIGMFGIPL
                     GHFVADYFGEVYFFSKIAMGILGMVILGIWLAEIKLNLNDLKKSIKP"
     gene            complement(917..1222)
                     /gene="nuoK"
                     /locus_tag="WGO87_001062"
     CDS             complement(917..1222)
                     /gene="nuoK"
                     /locus_tag="WGO87_001062"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0392431.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLNKLKG"
     gene            complement(1219..1890)
                     /locus_tag="WGO87_001063"
     CDS             complement(1219..1890)
                     /locus_tag="WGO87_001063"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0392432.1"
                     /translation="MTFSAILFYILATIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTINPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(1902..2381)
                     /gene="nuoI"
                     /locus_tag="WGO87_001064"
     CDS             complement(1902..2381)
                     /gene="nuoI"
                     /locus_tag="WGO87_001064"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0392433.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(2462..3538)
                     /gene="nuoH"
                     /locus_tag="WGO87_001065"
     CDS             complement(2462..3538)
                     /gene="nuoH"
                     /locus_tag="WGO87_001065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0392434.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            complement(3541..5802)
                     /locus_tag="WGO87_001066"
     CDS             complement(3541..5802)
                     /locus_tag="WGO87_001066"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0392435.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVHSAIKCIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAVHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQIAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(6090..6527)
                     /locus_tag="WGO87_001067"
     CDS             complement(6090..6527)
                     /locus_tag="WGO87_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392436.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            complement(6527..7828)
                     /gene="nuoF"
                     /locus_tag="WGO87_001068"
     CDS             complement(6527..7828)
                     /gene="nuoF"
                     /locus_tag="WGO87_001068"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0392437.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALAQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(8218..8691)
                     /gene="nuoE"
                     /locus_tag="WGO87_001069"
     CDS             complement(8218..8691)
                     /gene="nuoE"
                     /locus_tag="WGO87_001069"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0392438.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVAEYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(8691..9947)
                     /gene="nuoD"
                     /locus_tag="WGO87_001070"
     CDS             complement(8691..9947)
                     /gene="nuoD"
                     /locus_tag="WGO87_001070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0392439.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKKQPYEVYGKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     YHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(9937..10530)
                     /locus_tag="WGO87_001071"
     CDS             complement(9937..10530)
                     /locus_tag="WGO87_001071"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0392440.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRHILTDYGFVGHPFRKDFPISGY
                     VEMRYDEAQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(10543..11025)
                     /locus_tag="WGO87_001072"
     CDS             complement(10543..11025)
                     /locus_tag="WGO87_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0392441.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(11016..11372)
                     /locus_tag="WGO87_001073"
     CDS             complement(11016..11372)
                     /locus_tag="WGO87_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0392442.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         2899 a         3015 c         2816 g         2823 t
ORIGIN      
        1 acggtttaat acttttcttc aaatcattca aatttagttt tatctcagcc agccaaatac
       61 cgagtatcac catccccaat attcccattg ctattttcga gaaaaaataa acttccccaa
      121 aataatctgc tacaaaatgc cctagcggaa taccgaacat tcctattacc aatgcaacaa
      181 cgggagggga ttttaaagtc tttttcaatc cggatatgaa attactatta ttcaataaca
      241 acccgacccc gaccgaattt ccgaataaag aacttcccac gtatgccgaa ataagcactt
      301 gcgccgcctc atctccaaac aaggttgcaa ctatgggaag ccccaaccat ccgatgttca
      361 aatagcacaa acacaaattc caaacaggat ctttatcaac tcttttcccg attaggataa
      421 tcaaccccat cgaaataatc atccataaca taacatactg cataccatca cgatatgtcg
      481 ttatgttata gacaataaca aaagggataa tccatctgct caatattaca gaaaaagtgc
      541 ctttaatttt tctaaaaggg gttttcccaa tcaaaatgcc aataaacaga tataccaatg
      601 gaagagcaat tgaaaatgac ataatcactc tccactttct gaaattctaa aattttcaaa
      661 aacccattgt tttacccaat tacccgccat acagcccaat ggctgccaat gagcccttga
      721 aattgtaaac attctctttc actcatacta atccacaatt tatgcatata tgggcgtgtg
      781 gagaccaaag tgccttgata tggcaaacaa gagtttttac ctgtaactgt ttgattaagc
      841 taactcctgc tgcacctaat ccaacagcag caatatcaaa cattatctat ctcctttttt
      901 ctttaaaaat tccaaatcaa cctttaagtt tgttcaaatc ggcaacgttg attgtttgtc
      961 ggttgcggta caccaatacc ataatcgcca aaccgatggc agattcggca gcggcaacgg
     1021 tcagtacgaa gaatacgaaa atctgtccgg cggtatcgcc caaatgttgc gagaaggcga
     1081 taaagttgaa gttcaccgcc aaaagcatca gctcgatgga catcagcaag accagcacgt
     1141 ttttgcggtt cataaagatg cccatcgcgc tgataccgaa caggagcgcg cccaatacca
     1201 gataatgcgt caaggtaatc atgctttgcc ctccccttcc ggcttgagac cgtctgaaac
     1261 ttcgctttct tcggcagatt cgacttgcgg tttgaccgct tccattttca ccagacgcat
     1321 acggccttgg tcggcgcgta ctttaacctg gtcggcagga tccatgcgtt tcggattgat
     1381 ggttttacgg tgaaccagcg caatcgccgc caccataccc aacaacagca ataccgccgc
     1441 caattcaaac ggcaacagat agtcggtata aatacggctg cccaaatcgc ggatattgtt
     1501 gtaatcggca ggaatgtctt tcatcagacc aaatgcggca aggtcggttt tcgggttgac
     1561 cagaatcagg atcagcgcaa ccgccaacaa tgtgccgacc acaccggcaa caggcgcgtg
     1621 ccgccagaaa ccggcacgca tttcttcaat gtcgatgttc aacatcatca cgacgaacag
     1681 gaacaacacc atcacggcgc cgacgtaaac caccaccagc gtcacgccca aaaactcagc
     1741 ctgcatcagc atccaaagca tcgcgctcac gcagaaggtc agcaccagat gcaaagcggc
     1801 gtggacaggg tttttagcgg taacggtacg cagcgcgccg tataagacga tggtggcaag
     1861 gatatagaac agaatcgcgg aaaaagtcat tcgtctgctc cttaacggta cggcgcgtca
     1921 gcggctttgc gtttggcgat ttcggcttcg tatttgtcgc cgattgccaa gaggatcggc
     1981 ttggtcatgt gcaagtcgcc ttttttctcg ccgtggtatt caaaaatatg cgtttccaca
     2041 atcgcatcgg ttgggcaggc ctcttcgcag aagccgcaga agatgcactt ggtcaggtcg
     2101 atgtcgtaac gcttggtgcg gcgggtgccg tcttcgcgtt cttccgattc gatattgatc
     2161 gccattgccg gacacaccgc ctcgcacaac ttacacgcaa tgcagcgttc ctcaccgttc
     2221 ggataccgcc gctgcgcgtg caggccgcgg aaacgcacgg attgcggcgt tttctcttcg
     2281 ggaaaataaa tcgtgtcttt gcgggcgaaa aagtttttga gcgttacgcc catacctttt
     2341 accaattcgc caagcagaaa ggtttttact aagttagcca tattatgttc cctcaaaaca
     2401 gggatttcgt taggtattca aaatcgcttt gttcagacgg cctcaagatg ccgtctgaaa
     2461 cttatttcca caaattcagc ggtgaaatca tccacacgcc caaaaccacg atgtaggcga
     2521 agccgatcgg aatcagcact ttccagccca aacgcatgat ttggtcgtaa cggtagcgcg
     2581 ggaaggttgc acggatccac aggtaccagt acagaaccgc cgccattttc acgaacatcc
     2641 agaatgcgga aggcgtaccg ataaagcccc agctttgcgg gaacggagac aaccagccac
     2701 cgaggaacat caacgatgtc agcgcggcaa tcagaatcat gaaaatgtat tcggcaagga
     2761 agaacagcgc gaacgcgaag ccggaatatt cgacgtggtg accggcaacg atttcagact
     2821 caccctctgc cacgtcaaac ggcgcacggt tggtttcggc aacggcggaa atcagataaa
     2881 cgatgaagat ggggaagagc ggcagccagt tccacgagaa taccgaaccg ccggcaatac
     2941 ctttggcttg cgcggcaacg atgtcggaga agttcatact gcccgatacc atcacgacgc
     3001 acaccagcgc ggcactcatg gcgatttcgt aagaaatgct ttgcgcggag gcacgcattg
     3061 cgcccaggaa cgaatatttg gagttggaag cccagcccgc gatgatcacg ccgtaaaccg
     3121 acagcgaggt aatcatcagg atgtacaaaa ggccgatatt gatattggtc agcacccatt
     3181 cttcgttgaa cggaatcact gcccatgccg caaaagacgg ggcgagcgac ataatcgggc
     3241 cgatgtagaa cagggctttg tttgacaact tcggacgggt tacttcttta aacaagagtt
     3301 tgaacacgtc ggcaaacggc tgaatcagac cccacgggcc ggttacgttc gggccgacac
     3361 gaagctgcat aaagccgatg actttacgtt cgaaatacgt caggtaggca acggtcagaa
     3421 tcagcggaat caggataatc acaattttga cgatgacgga taccaccaag cctacggtaa
     3481 tacccaaatc gcccagaccg agcgttgcgg caaagaggtt ttggaaccat tcttgcatga
     3541 tcaagctccc gccagttcaa tagtgtccat caacgcaccc agcgcggcat tttcggtatg
     3601 cagcggcaga tgcaccacgt tttcaggcaa accggcatcg gctttgacgg caaccgatac
     3661 gcttgcgccg ttttgtttgg cgacagcaat ttgtccgtct tgcaggccca agcgtgccaa
     3721 agtgttcgga tttacacgcg cggcaggcac ggcggcatgg ctggtttctt gcaacggtgc
     3781 ggaacggcgc acgatagaat cagtgtgata aataccgacg ccgccgacac ggacaaggcg
     3841 gtctgaggcc gtctgaacgc cctcccctgt ccatgtgcta cggttgtcca gtttggacgg
     3901 caggctttct gcatccagcg cgtctttcag aatcgcagcg gtatcgtgat attcaaaacc
     3961 tttcaggtca aacagattgc ccaatacgcg caatactttc cacatcggac gcgaatcgcc
     4021 gaagccttgt accacgccgt ggaaagattg cagacggcct tccatattga tgaagctgcc
     4081 tgaggtttcg gtaaacggtg caatcggcaa caacacgtcg cacacgtcca gcagcgtttc
     4141 gctgacaaac ggcgtaaacg ccatcacgct ttttgcctgt ttcaacgcgg ctacggcttt
     4201 tgcaccgtca accgtatcaa tttccggctc gacgttgagc aacaagactg cctgtttcgg
     4261 cgcgtttgcc atttcggcaa cgctctcgcc ggagtttaca ttcaagacat ccgcaccaac
     4321 actgttggcg gcttgcggca aaatacccag cactgcgccg gtcgcgtccg ccagctcttg
     4381 cgcggcggca taaatggcgg cgtaatcagg atggttttgc acttccgcgc ccaaaatcac
     4441 cgctgctttt tcagcgtttt tcaggctggc ggtaacggcg tgttccgcat cggcagacag
     4501 gtttttcaga cgacctgccc actcgtcggg atgtacggct tcttgagaca gaagcggcat
     4561 aaacaattct tctttactgc ttgccaatac gctcaaagcc atgcggtctt tagcggcgcg
     4621 gcgcaggcgg gcggtcagaa gcggctgttc tttgcgcagg ttcgcaccga ctaccaatac
     4681 ggcatcgttg tccgccagcg attcaatgct ttgtcccaac cattgcgcac ctttaatgcc
     4741 gtctgaaaga cgtttgtctt gttggcgcag acgggtggca aagtttttaa cacccaagcc
     4801 gtcggcaagc tttttggcca aatacagctc ttcaaccgta ttcatcggat tggcccaaac
     4861 gccgacttga ttttggttgc cgtctttggc gatacattta attgcgctgt ggacatattc
     4921 caacgcggtt ttccaatcca cgtccatcca ctcgccgccc tgtttgattt tcgggttttt
     4981 caaacggctt tcgtgataca ggccttcata ggcaaaacgg tcgcggtcag acagccaaca
     5041 ttcgttaatc gcttcgtttt ccagcggcaa cacgcggcgg acggtatggt ctttggtttg
     5101 tataatcagg ttgctgccca aagcgtcatg ggcggaaacg gatttgcggc ggttcaattc
     5161 ccaagtacgc gcgttgaagc ggaacggttt gctggtcaat gcgccgacag gacacaaatc
     5221 gatgacgttg cccgacagct cggtttccac cgttttgccg ataaagggca tgatttcgga
     5281 gtgttcgccg cgattcacca tcgcaatttc ctgcaaaccg gcgatttctt cagtgaaacg
     5341 aacgcagcgg gtgcagtgga tacagcggct catttcctcg gcggaaacca aaggccccat
     5401 gtctttgccg acgacggaac gtttttcttc ggtgtagcgg ctggtggttt ttccgtagcc
     5461 caccgccaaa tcctgcaact ggcattcccc gccttggtcg caggtcggac aatcaagcgg
     5521 atggttgatg agcaggaact ccatcacgcc ttcctgcgcc tctcgggctt ttgccgaatg
     5581 cgtacgcaca atcatgccgt ctgtaaccgg cgtggcacag gcaggcaggg gttttggggc
     5641 tttttctacg ttcaccagac acatacggca gttggcggcg atggaaagtt ttttgtggta
     5701 acagaaatgc ggaatataag taccgagctt gtgcgcggct tcaatcaccg tcgcgccctg
     5761 ctccacagat acttgtttgc cgtcgatttc gatttgtaac atggttcgtt cctagttacg
     5821 ggtatttgat aaataatctt atggaggtcg tctgaaaaat gggtttcaga cggccttttg
     5881 agtttcattg acaatcaata ctgtttaaaa cgtttactgc caccttgccg tcattcccgc
     5941 gtaggcggga atccattttt tgaatttcgg caactgcttt tcaaatatcg gtttctttag
     6001 attccactat ggattcccgc ctacgcggga atgacggcaa agttaaattt ttagcatttt
     6061 gcttttaacc aatataaaac caactaaaac tataacttat ttattatatt tttaaatgac
     6121 ttaaataact ctttaacagc acgctcattg tcttcatctg taggctcaaa cttttcacac
     6181 caattaatta tatttacatc gccatgcaat aactctgcat aacatcctac tgcatcgcaa
     6241 ctaccacccc gtgtccttga cgtgccaaaa ataaaatcac aattatgcga ataaagtttg
     6301 tataaatttc tcctaacctc gtattcatta tcaccagatg aataaaaacc aattctctga
     6361 ccttcatgat caaatactgc aacaaaatct aagccgtttc catgtctttc aaaaaagact
     6421 agaaatttag aaaatttccg acaaatctga ttaaaaagcg tattgagtgt cgtactctta
     6481 cctttgttgg ctgcaccgta tagaataaaa attttagctt ccaccattac caccctcccc
     6541 acttatgctc tttcatcggt ccgccgtgtt cgatgtaatg cacaaactca tcacggaaat
     6601 gcttggtaaa gctgcggaca gggaagacgg cggcatcggc gagggcgcag atggtgcggc
     6661 ctgccatttg gttgccgaca gaatccagca aatccaaatc ttccatttta cctttgcctt
     6721 ccacgatacg gtggacgatg cggtagagcc agcccgtacc ttctcggcaa ggcgtacatt
     6781 ggccgcaaga ctcgtcgtag tagaagtagc tcaggcgctc aagggctttc accatgcaca
     6841 cgtcttcgtc catgacgata atcgcgccag aacccagcat cgagcctgct ttggaaatcg
     6901 agtcgtagtc catatttgtc tgcatcatga tgtcggcagg caatacgggc gcggacgaac
     6961 cgccaggaat aacggccttg agttttttgc cgccgcgcat accgcctgcc attttcaaaa
     7021 cttcggcaaa cggcgtgccc aacggcactt catagttgcc cggacgctcg acatgaccgg
     7081 aaatacagaa taatttggta ccgcctgcat tcggaatacc tttatcggca aatgcctgtc
     7141 cgccatcacg gataatgaag gggacggaag agaacgtttc ggtattgttg atggtggtcg
     7201 gcttgccgta caggccgaac gaagcaggga acggcggttt gaagcgcggc tggccttttt
     7261 tgccttccaa cgattcgagc aatgcagttt cttcgccgca aatatatgcg ccgtagccgt
     7321 ggtgggcgaa aagttcgaat tcaaaatccg aacccaaaat atttttaccc aaaaaacctg
     7381 cggcacgcgc ctgcgccaaa gcggcttcaa aacgttggta gccttcgaag atttcgccgt
     7441 ggatgtagtt gtagccggct ttcgcaccca tcgcgtaacc ggcaataatc atgccttcaa
     7501 tcaaggcatg ggggttgaac ataatgatgt cgcggtcttt aaatgtaccc ggctcgcctt
     7561 cgtcggtatt gcaaaccaca tatttttcgc ccgggaagga acggggcata aagctccatt
     7621 tcagaccggt cgggaagccc gcaccgccgc gaccgcgcaa accggaggtt ttgacttcgt
     7681 caatcacatc ggtttgcgag atgttttcgg acaggatttt acgcagggcg gtataaccgc
     7741 cacgtttgac gtattcgtcc aatgtccagc aatcgggatt ggcggtatcc acttggtcaa
     7801 aaatcacgcc tgattggtaa atagccattt ttggtgtgcc tgtttgtttt cgtatcggtt
     7861 gcggccgctg tttcagacga ccttaagatg tctttgtgta ccggcttgta acgtcgtctg
     7921 aaataaaatc tagtttatca aatcgatatt ttcagacgac ctttaatcgt ttttgttgga
     7981 tctcgaccac ttgcttgtct gtcgtcattc ccgcgcaggc gggaatccat cctcaatggt
     8041 aagcaatgtc ttattaaatt cagaaaccga atcttaccgg tggattcccg cctgcgcggg
     8101 aatgacggca tttcggtatt tcagtagggc ggattcttaa atccgacatt ttgccttttt
     8161 acccactctg tcggctacaa gtatccgacc tacgtttaaa tcgtcgtttc agacgaccta
     8221 ctccaactcc gccagtttct tctcaatcgc ttcttcggtc ataaagctgc acatgctgtg
     8281 gttgttgacc agcataacgg gagcgtcgcc gcacgcgccc atgcattcgc cttcgacaag
     8341 agtaaatttg ccgtcgggcg tggtttcgcc gtagccgata ccgagtcttt gtttgaggta
     8401 ttcgccggta gccataccgc cgcgcagggc gcagggcagg ttggtacaaa cggtcaattt
     8461 gtatttgccg acaggctcaa ggtcgtacat attgtagaag gtagcgactt cgtaggcttg
     8521 tgcaggcgag atgccgatgt attcggcaac aaaagcgatg gtttcgggag cgagccagcc
     8581 tttttcggtc tgggcaatac gcaatgcacc cataatggcg gagcggcgtt ggtcggcagg
     8641 gtatttcgcc aactctatgt cgatttgttt taaggattct gcggataaca ttatcggtca
     8701 acctccccga atacgatgtc ctgcgtaccg atgatggcaa cgacatcggc gagcatgtgg
     8761 ccttttgcca tttcgtccat gccttgcagg tgggcgaagc cgggtgcgcg gattttcagg
     8821 cggtagggtt tgtttgcgcc gtctgaaatg atgtaaacgc cgaactcgcc tttcgggtgt
     8881 tcgacagcgg tgtaggtctc gccctcggga acgtgcatcc cctcggtaaa gagtttgaaa
     8941 tggtaaatca ggtcttccat acctgttttc atttcggtac gtttgggcgg agcaaatttg
     9001 tggtttgtgg taatgaccgg acccggatta acacgcaacc actcggaaca ttgtttgatg
     9061 atgcgtacgg attgacgcat ttcttccata cggcagaggt agcggtcgta gcagtcgccg
     9121 ttgacaccga cggggatgtc gaaatccatt ttgccgtaca cttcgtaagg ctgtttctta
     9181 cgcacgtccc attccacgcc cgaaccgcgc aacatcacgc cggtaaagcc tttttgcatg
     9241 gcgcgttcgg gggagacgac gccgatgccg acggtacgct gtttccaaat acggttgtcg
     9301 gtcaggaggg tttcgagtgt gtcgatattt ttcgggaaac gttcgcagaa ggcatcgata
     9361 aagtcgagca tcgtgccttc gcgggattcg ttgagctgct tcaatacttt ggcattgcgg
     9421 aatttgctgc cctcgtattt aggcatgaag tcgggcaggt cgcggtaaac gccgccggga
     9481 cggaagtagg cggcgtgcat acgcgcgccg gaaacggctt cgtacaagtc catcagctct
     9541 tcacggtcgc ggaaggcgta aagaatggcg gtcatcgcgc cgatgtcgaa ggcatgcgaa
     9601 ccgatgccca tcaagtgatt gaggatgcgc gttacttcgg caaacatcac gcggatgtat
     9661 tgggcgcgga taggcacatc gataccggca agtttttcta ccgccaagca atacgcctgc
     9721 tcgttgacca tcatggaaac gtagtccagc ctgtccatat aaggcagggc ttgcaggtag
     9781 gttttggttt ccgccagttt ctctgtgcct cggtgcagga ggccgatatg cgggtcggca
     9841 cggacgattt gttcgccgtc cagctccaaa atcatacgca atacgccgtg cgccgcaggg
     9901 tgttgcgggc cgaagttgat ggtgtagttt cttaatttat tggccaccgt agttctcctc
     9961 acggacgata cgcggcgtga tctcgcgcgg ctcaatggta acaggttggt aaatcacgcg
    10021 tttttgcgct tcgtcgtaac gcatttccac atagccggaa atcgggaagt ctttgcggaa
    10081 cggatgtccg acgaagccgt aatcggtcag gatgtggcgc aggtcgggat ggttgttgaa
    10141 catgatgccg tacatatcaa aggcttcgcg ttcgtaccaa tccgcgctgt tgtaaatatc
    10201 gactacggat tcgactacgg ggaagtcgtc gtctgaaacc cagacgcgta cgcggatgcg
    10261 ttgattgttt ttaacggaaa gcaactgact gacgacggca aagcgtttgc cctgccatac
    10321 ttcgtttttg taagtgctgt aatcgacacc gcacaagtcg accagaagct cgaaatgcaa
    10381 ctcttcatgg tcgcgcaatg cggtcatgac tgaaatatag tgttcgggca gacactcgac
    10441 ggtaatctca cccaaagcgg aaatgacttt gcctgcctga ttgcccaaaa cgcggctgac
    10501 ggtttcgtat aagttttgaa tgcttgccat atcgtcctct ccttactcgt cacgcgcgat
    10561 ggtggaagtg cgcttgattt tttgttggag ctgaatcaga ccgtaaatca gggcttccgc
    10621 agtcggcgga caacccggca cataaacgtc taccggcacg acgcggtcgg caccgcgcac
    10681 aacggaataa gaatagtgat aataaccgcc gccgttggca cacgagccca tagacaatac
    10741 ccagcgtggc tcggcgagct ggtcgtatac gcggcgcagg gcgggcgcca ttttgttggt
    10801 cagcgtaccc gccacaatca tcaggtcggc ctgacgtggg gacggacgga aaatgatacc
    10861 gaaacggtca aggtcataac gcgccatacc cgcatgcatc atttccacgg cgcagcaggc
    10921 caagccgaaa gtaaccggcc acaacgaacc ggtacgcata tagttcaaca ccgtatccgc
    10981 gctggtggtg atgaaacctt ttttcaaaac gccttctatt cccattccag cgcacctttt
    11041 ttccattcgt aaacaaagcc taccgtcaga acgacgataa acaccaacat agaccagaag
    11101 ccgtacgcgc ccaaatcttt gaacacaact gcccacggca acatgaacgc gacctccaaa
    11161 tcaaacagga tgaagaggat ggcgacgagg taatagcgca cgtcaaactt catcctcgca
    11221 ttttcaaagg cttcgaaacc gcattcgtaa ggcgcgtctt tttcggcata gtggcgtttc
    11281 gggcctaaaa tcgtgccgag caggataaac agtacgccgg ccgcgaggcc gacgaggata
    11341 aagacaaaga cgggaaagta agcggacaac atggttacac ccaaatccgt taacaaaatt
    11401 tctacaataa tttcgtattt tagcgaattt caaaaaccat taaaaggtaa atatcggcaa
    11461 aacgcccaaa aaaacccaat aaatacaatc atgttatgat aacgattctt atttgatttt
    11521 ataaggtcat ataaattttt acaccgtaat tcg
//
LOCUS       DAWFBO010000058        11516 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFBO010000058 DAWFBO010000000
VERSION     DAWFBO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.058"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            122..544
                     /locus_tag="WGO87_001074"
     CDS             122..544
                     /locus_tag="WGO87_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392443.1"
                     /translation="MKKTFLSAVLLLSAATQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGWGRGRWATMKFQNGEFMYTVDAGFDSV
                     THAVGSGVLVERRGKEIARFDCTSKTAQANFDDDDFAW"
     gene            562..1323
                     /locus_tag="WGO87_001075"
     CDS             562..1323
                     /locus_tag="WGO87_001075"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0392444.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEMAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            1338..2672
                     /locus_tag="WGO87_001076"
     CDS             1338..2672
                     /locus_tag="WGO87_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0392445.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLEIAVSGLPLILDGVLKPSVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAETAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTTAENQEAEQATSEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            2669..3886
                     /locus_tag="WGO87_001077"
     CDS             2669..3886
                     /locus_tag="WGO87_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0392446.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            3961..5025
                     /locus_tag="WGO87_001078"
     CDS             3961..5025
                     /locus_tag="WGO87_001078"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0392447.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     MQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            5211..6590
                     /gene="radA"
                     /locus_tag="WGO87_001079"
     CDS             5211..6590
                     /gene="radA"
                     /locus_tag="WGO87_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0392448.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMSELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(6866..7345)
                     /locus_tag="WGO87_001080"
     CDS             complement(6866..7345)
                     /locus_tag="WGO87_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0392449.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTIGE"
     gene            complement(7477..7836)
                     /locus_tag="WGO87_001081"
     CDS             complement(7477..7836)
                     /locus_tag="WGO87_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0392450.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(7875..11489)
                     /gene="recB"
                     /locus_tag="WGO87_001082"
     CDS             complement(7875..11489)
                     /gene="recB"
                     /locus_tag="WGO87_001082"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0392451.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSNDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRAYACEKDGITMLKRNWQRVTDNAPADTDFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEMWLPAVAEM
                     AEACRKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLGADASAYTQQAMDEAVTHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGTDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         2487 a         3045 c         3429 g         2555 t
ORIGIN      
        1 gtgccgccct atgtgttgcc ctaatttgga aggggttaca cccttttcaa ataaaatctg
       61 atgctgctgc cacgaaggac ggatgtccaa gtggcggggt tttaaccatt aaggaaatac
      121 gatgaaaaaa acgttccttt ctgccgtatt gcttctgtca gctgcgaccc aaaccgtgtg
      181 ggcagatacg gtgttttcct gtaaaacgga caacaacaaa tatatagaag ttcaaaaaat
      241 taaccgcaat ctttacgaat attcgttcgg cagtgcggca aaaaaagaaa ttgccatacg
      301 caacagcaaa gctgatctgt tggggcgttc cgacaggtgg caaggctggg gcagaggccg
      361 ttgggcgacg atgaaattcc aaaacggcga atttatgtac accgtagatg caggcttcga
      421 ttccgtgacc catgcagtag gcagcggtgt ccttgtggag cgtaggggca aggaaatcgc
      481 acggttcgac tgtacgtcga aaaccgcgca ggcgaatttc gacgatgacg attttgcctg
      541 gtaatcgggg cggataaggc gatggaaaca gtgaaaccca tcatgctgat tgtccgaccg
      601 tcgggcaggg cgaaggacga tgtggaaacc tgccgtcgtg ccggatggca ggcggaagtg
      661 ttgagcccga ttgaaatcga agcagatgct gccggactgg aacttttgtc cgaacaatat
      721 gcccgtgcgg atgccgtgtt ttgggtcagt ccgaccgccg ttgaaaccgc cgtcccgtac
      781 cttaaccttt cagacggcat aaaggcgcag attgccgtag ggcagggcag ccgccgcgca
      841 ttggaacgct gtttggtcag aacggtcatc gcgcctgatg acggcaacga cagcgaggcg
      901 gttttgcgcc tgccggtttg gaacagtctg cccgaaggtg cgcgcgtatt gtttgtgcgc
      961 ggacacggcg ggcgggattt tctgatgaat gccttgcagg agaaaggttt tcggacggag
     1021 atggcagaag tctatttcag acggcataaa cctttgaact ttcaaaattt ccaaaccgaa
     1081 aatattgccg ccgcctatat tacgtcgacc gagctggtgc gcttgctgtt cgggcagctt
     1141 ccgccgcaat tttcccgatt cttcaaatcc ttgctatact ttacccatca tccgcgcatt
     1201 gcggaggcat tgaagcgcga aggcgtgtgt tcggtcgaaa ccgtccctac gctggaagcc
     1261 gcgctttccc attcttccat ttccgtttca gacggcatgg tctttcccgg aacctcaaat
     1321 taataaggag caaaacggtg ggcgaacctg aaaacaaatc atccgaaccc gtacgcgaga
     1381 tacaggcatc aaaagaaatg ccgtctgaaa cctcttcccc acgcaaagaa aacgaaacag
     1441 aagtacacat tcctgccgct ccttttatcg tcaaacagtc cggcagcaac gctttggcag
     1501 tctgcgccct ggtattggcg gcattgggtt tgggtacaag tggttttttg tttgtccaag
     1561 gacagaatgt cttgaaaaac caagagctgg cattcaacca aaaaatcgac aaagccgctt
     1621 tgggcgagtc ggaaaacgcc gccctgctca aagacaacct caaccggcaa gccgccatac
     1681 aatcagagct cgaccgtttg gacggaaacg tcaaagcaaa cggcgaacaa atcttggaaa
     1741 tgcaaaaatc ctatcgcgag ttgaccaaag gacgcgccga ttggctggtg gacgaaaccg
     1801 agaccatact caatctggcg gcgcaacagc tggtgttgac tggcaatatc caaacggcag
     1861 tcggcgtatt ggagcatatc gacagccgcc tgtcccgttt caatcaggca gagcttctgc
     1921 cgatcaagca ggcggtcagc agcgacttgg cggaactgaa aaaccgtccc tatgtcgata
     1981 tttccggcac ggcattgcgc ctcgacaggc tggaaatcgc cgtatccgga ctgccgctga
     2041 ttttggacgg cgtattgaaa ccgagcgtac aggcacgcaa cgaagccgtt tccgcttcat
     2101 ggtggcagaa cgtatgggaa aaatccctcg gcacattgaa ggggctggtc gaaatccgcc
     2161 gtttggaaaa caacgatgcc atgctgattt ctcccgaaca ggcctatttt gtgcgtgaaa
     2221 acctgcgtct ccgccttttg gatgcgcgga ctgcattaat gcagcgcaac agcgaagtct
     2281 atcagggcga tttgaacaat gccgaaaccg ccgtcagaca gtattttgat gccaaatccc
     2341 ccgccacgca gtcgtggttg aaggaattgg cggaattgaa ggcgttggat gtgcgcatga
     2401 cggcggatga cggtttgaaa aacagcctaa atgccgtccg cgcctatcgc gacggtacgc
     2461 gcatgacgac ggcggaaaat caagaagcgg aacaggcgac ttccgaaccg gcaaacgaaa
     2521 acacagcttc cgaaccggct gccgcatcgg atgtgaagac catagaagca ccgtccctgc
     2581 cttcggaacg caaaccggaa cagcctgcaa aaaaacagcc cgtaccggaa aaggcagggc
     2641 gttcgccgtc cgctaaagga gaacgcgcat gaaaacggta gtctggattg tcgtcctgtt
     2701 tgccgccgcc gtcggactgg cactggcttc gggcatttac accggcgacg tgtatatcgt
     2761 actcggacag accatgctca gaatcaacct gcacgccttt gtgttaggtt cgctgattgc
     2821 cgtcgtggtg tggtatttcc tgttcaaatt cattatcggc gtattcaata tccccgaaaa
     2881 gatgcagcgt ttcggttcgg cgcgtaaagg ccgcaaggcc gcgcttgcct tgaacaaggc
     2941 gggtttggcg tattttgaag ggcgttttga aaaggcggaa ctagaagcct cacgcgtgtt
     3001 ggtcaacaaa gaggccggag acaaccggac tttggcattg atgttgggcg cacatgccgc
     3061 cgggcagatg gaaaacatcg agctgcgcga ccgttatctt gcggaaatcg ccaaactgcc
     3121 ggaaaagcag cagctttccc gttatctttt gttggcggaa tcggcgttga accggcgcga
     3181 ttacgaagcg gcggaagcca atcttcatgc ggcggcgaag atgaatgcca accttacgcg
     3241 cctcgtgcgt ctgcaacttc gttacgcttt cgacaggggc gacgcgttgc aggttctggc
     3301 aaaaaccgaa aaactttcca aggcgggcgc gttgggcaaa tcggaaatgg aacggtatca
     3361 aaattgggca taccgccgcc agctggcgga tgctgccgat gccgccgctt tgaaaacctg
     3421 cctgaagcgg attcccgaca gcctcaaaaa cggggaattg agcgtatcgg ttgcggaaaa
     3481 gtacgaacgt ttgggactgt atgccgatgc ggtcaaatgg gtcaaacagc attatccgca
     3541 caaccgccgc cccgagcttt tggaagcctt tgtcgaaagc gtgcgctttt tgggcgaacg
     3601 cgaacagcag aaagccatcg attttgccga tgcttggctg aaagaacagc ccgataacgc
     3661 gcttctgctg atgtatctcg gtcggctcgc ctacggccgc aaactttggg gcaaggcaaa
     3721 aggctacctt gaagcgagca ttgcattaaa gccgagtatt tccgcgcgtt tggttctggc
     3781 aaaggttttt gacgaaaccg gagaaccgca gaaggcggag gcgcagcgca acttggtttt
     3841 ggcaagcgtt gccgaggaaa accgaccttc cgccgaaacc cattgaaacc ttcagacggc
     3901 atcgttgaaa tgccgtctga aaaccatttc acttgacaaa cagactgaca gaaaacccgt
     3961 atgactcttt taaaaaacga taccttcctc cgcgccctgc tcaaacagcc cgtcgaatac
     4021 acgccgattt ggatgatgcg ccaggcgggg cgttatctgc ccgaatacaa agccacacgc
     4081 gcgaaagcgg gcagcttcct cgatttgtgc aaaaacaccg aattggcgac cgaagttacc
     4141 atccaacctt tggaacgctt cgatttggac gcggcgattt tgttttccga catcctgacc
     4201 gtccccgacg caatgggctt gggactgtat tttgccgaag gcgaaggccc gaaattcaaa
     4261 cgcgccatgc aacacgaggc cgacatcgcc aagctgcacg ttcccgatat ggaaaaactg
     4321 caatacgttt tcgacgcggt aacttccatc cgtaaagcat tggacggccg cgtaccgctt
     4381 atcggctttt ccggcagccc gttcacactc gcctgttata tggtcgaagg cggcggcagc
     4441 aaagaattcc gcaccatcaa aaccatgatg tactcgcgcc ccgatttgct gcacaaaatc
     4501 ctcgatacca acgcccaagc cgttaccgcc tacctcaacg cccaaatcga cgcgggcgcg
     4561 caggcggtgc aaattttcga cacttggggc ggcgtgttga gcgacgcggc gtttaaagaa
     4621 ttcagcctca aatacatccg ccagatcgtc gccggactca aacgcgaaag cgaaggccgc
     4681 cgcgtgcctg ttatcgtatt tgccaaaggc ggcgggctgt ggctggaaag tatggcccaa
     4741 atcggcgcag acgcattggg cttggactgg acgtgtaaca tcggcgaagc acgccgccgc
     4801 gtcggcaagc aagtcgccct gcaaggcaac ttcgacccgt tcgccctctt cggtacgccg
     4861 gaatccatcc gcaccgaggt cgcacgtatc ctagccgact acggacacgg cagcggccat
     4921 gtcttcaacc tcggacacgg catcaaccaa cacgccgacc ccgaacacgc caaaatctta
     4981 gtcgataccg tacacgagct gtctcggcag tatcacggcg ggtaagccgg caggaaaccg
     5041 cccgatatgc cgtctgaagc cgagagatgg ccggttaggg taaaaataag gcaatgcggc
     5101 aatatccgcc gtgtacggat agtacatgac ggcggcgttg tcgtattggc gcaatcccaa
     5161 ccgtccctat gttcagacgg catttttgtt ttcagatgca gggaaaaccg atggcaaaaa
     5221 cgcttaaaac cctttaccaa tgcaccgaat gcggcggcac ttcgccgaaa tggcaaggca
     5281 aatgcccgca ttgcggcgag tggaacacgc ttcaagaaag cttagccgcg cccgagccga
     5341 aaaacgcccg cttccaatct tgggcggcgg atacctcgac cgtccaatcc ctctccgccg
     5401 ttaccgctgc cgaagtgccg cgcaacccga ccggcatgag cgaactcgac cgcgtattgg
     5461 gcggcggttt ggtcgatggc gcggtcatcc tactcggcgg cgatcccggc atcggcaaat
     5521 ccacgctgtt gttgcaaacc atcgccaaaa tggcgcaaag ccgcaaagtg ctgtacgttt
     5581 ccggcgagga atccgcccaa caagtcgccc tgcgcgcgca gcgtttggaa ctgcccaccg
     5641 aaggcgtgaa cctgcttgcc gaaatccgca tggaagccat tcagacggcc ttgaaacagc
     5701 atcagcccga agtcgtggtt atcgattcca tccaaaccat gtattccgac caaatcactt
     5761 ccgcccccgg ctccgtgtcg caggtgcgcg agtgtgcagc ccaactgacg cgcatggcga
     5821 aacagatggg catcgccatg atactggtcg gacacgtgac caaagacggc gcgattgccg
     5881 gcccgcgcgt gctggaacac atggttgata ccgtgctgta tttcgagggc gaccaacatt
     5941 ccaactaccg catgatacgc gccatcaaaa accgcttcgg cgcggcaaac gaactgggcg
     6001 tgttcgcgat gacggaaaac ggtttgaaag gtgtgtccaa cccgtccgcc atcttcctcg
     6061 ccagctaccg cgacgatacg cccggctcgt gcgttttggt tacacaggaa ggcagccgcc
     6121 cgcttttggt cgaaattcag gcattggtcg atgacgcgca cggcttcacg cccaaacgcc
     6181 tcaccgtcgg actggaacaa aaccgtcttg cgatgctgct tgccgtgtta aaccgacacg
     6241 gcggcatcgc ctgtttcgat caggatgtgt tcctcaacgc cgtcggcggc gtgaaaatcg
     6301 gcgaaccggc ggcggatttg gcggtcatcc tcgcgatgct ttccagcttc cgcaaccgcc
     6361 ctatgcctga aaaaaccgtg gttttcggcg aaatcggctt aagcggcgaa gtccgccccg
     6421 tcgcacgcgg gcaagagcgg ctcaaagaag cggaaaaact cggcttcaaa cgcgccatcg
     6481 ttcccaaagc caatatgccg cgcaacgcca aagagtttcc gaacctgaaa atctacggcg
     6541 tttcgagttt gcaggaagcc atcgatattt gccgtgacag cagggaataa acggaaatgc
     6601 cgtctgaaat caggtttcag acggcatttg gtttgtggcg gattgaaaca agaaggcata
     6661 ccggcgacag ataagatttg tggcaaagtt gcctgtgatg tggcaaaaac acacacgccc
     6721 gtcatccccg cgagggcggg aatccggaat cgtccgtttc ggcaatgatt gaaaatcacg
     6781 gcaatccaac cgcttggatt cccgacttcg cgggaatgag gggcgtgtgc gtttgatttc
     6841 catccgccat atgtcggcaa cgggcttatt cgcctatggt tttttgtatc agtttttcgg
     6901 cgtttgccaa agtgtttgcc acttcgtcga aaccgatgcg gctgccggcg atgagggcgc
     6961 gcgtatcggt ataggcggcg cgtactttgc cgtccgtttc ggtaacgagg acgcgcaggg
     7021 gcagttgcag ggcgaaggcg gggtctttga ccatcagcgg cgtaccggct ttgggcgtgc
     7081 cgaagacgat gacttttgcc ggctgcatcg ttaagccgtt tcggcgggcg gcttcctgat
     7141 ggtcgatgac ggcaaaaatg tccatccctt tgctttttat ggcggtttca aggcggctga
     7201 cggtttcgtc aaaactgtat tttgaggtga gggtatgcgt ggtcatagcg gtttcgtttt
     7261 gggtggacgg ttcgctggca ggatgtgccg aagcggttga aatgcagagt gcggatgcgg
     7321 caatcagggg gagtatgtgt ttcatcgtat ttcctttttc cttttcggtt tgtttatcag
     7381 gcttcggacg gggcggcacg cgccgcgccc gatgttgcgc cgcgtgccgg aacgccgtat
     7441 gccgtctgaa agcctgtcct ttcagacggc attgcgtcat ttcatccctt ttttgagcag
     7501 gtcttcataa ccgccgtgat tggcaacatt tgtataacct gcttttttca gctcttgaag
     7561 ggcggcttcg gcacgccgtc cgctgcggca gtagaggttg acgggcgtgt ctttgtcggg
     7621 cgcggcttcg tgtatgcggc ggacgatttg gtcgacgggg atgttgaccg cgttgtgcaa
     7681 atgcccttcg ctaaattcct gttcggaacg gacatcgatc caaacggccg gatgttgcgc
     7741 ggtttgggcg gcggatacgg gtttttgcgg ggctgcctgc gcggcaaagg ctgctgaggc
     7801 aatgagtgcg gcggtaatca ggtgtttgat attcataggg ttctcctgcg gttgtcgttt
     7861 taagggacgg gaagttattt tatctgttcc aaagcggcgg catctatgtc ccaacgccaa
     7921 acgccgccgc ctttgccgtc tgtgccgcgc aaaaacaggt agcggacgga aacggcggcg
     7981 ggcggttgtc cgcgcagttt gaagtagcgt gcggcggcaa cggcgtaaat cagcgcctga
     8041 aggtaatagt ggtggtgcgt gacggcttcg tccatcgcct gttgcgtgta ggcggatgcg
     8101 tccgcgccga gatggtttga tttgtagtcg atgatgcaga tattgccgtc ggggtcttgg
     8161 cagaccatat cgataaagcc gtttaaaaag ccgttgacgg tgtggaagtc gagcgtttcg
     8221 gcggcggcac ggcagacttc gggcagcctg atgtcgtcgc gggcaaacca gtcgcgcagc
     8281 cgtttgaggc cgaagtcttc ggtatggagg gtaaagccca tttcgggaca gcggcactcg
     8341 ggcggaatgt cggacaggca gtatgccccc gtcagcggcg ttttacggca ggcttccgcc
     8401 atttcggcaa cggcgggcag ccacatttct tcaaacccgt atttcttcag cttgtcggca
     8461 atgagggttt cctgtccggc ggctgcttgt ccgaatttga aatcttcaag aatttcgtgc
     8521 aggcacagcc ccgcctgcgt gcctttcgga aaatcgtgta tcgatatgcc gtctgaagcc
     8581 gtcggcgttt cagacggcat cgccggcacc gaggtttcgg cggcatccaa ggacgggcag
     8641 gcatcttctt cgccgccgtc gggcgtttgg gtatggcggc ttaaagcggt aaagctggtg
     8701 tggcggacaa atcggaatct gcgttcggga atgctgtttg cggcaaattc ggcggtttga
     8761 ccggcgttgc cgcgataggc ggcagggggc ggcgcatctt ctgtgaaggc gaaatccgtg
     8821 tcggccgggg cgttgtccgt cacgcgctgc cagttgcgtt tgagcatcgt tatgccgtct
     8881 ttttcacacg cataggcacg gcggacggtt tcacggctgt cttgcggcaa gccttcaatc
     8941 aggtaggcga gggggttgtc ggcggtatcg gaagaatagg cggcgtagat gttgagctgt
     9001 tcctcggcac gcgtcagcgc gacataaagc aggcgcaggc gttccgccat ttcttcgtcg
     9061 gcgtattgct tctgttcgtc ttccgacagt tgcgcctttg ccaacagttc ggttcggttg
     9121 gcgctttggt ggaggatttg ccagtcggac ggcccggtat cttgcgcgtc ccacgcaaac
     9181 gggcagtaca ccagcggata ctgcaaacct ttcgaggcgt gcatggtaac gattttgacc
     9241 aaatcttcgt cgctttccag acggatggcg cggttgtcgt tgctgttgtt ttcggcaagg
     9301 ctgatttggt cgcccagcca cttgtgcaaa gcggcggggt tgcggttttg ggcgtcttcg
     9361 gcggcaagca gttcgagcag ttggaaataa ttggtcaggc tgcgcccgtt gttccggctt
     9421 aagaggcgcg tttcgatgcc gtgtgtttgg gaaaattgct gcatggcggc gaaaatgccg
     9481 tattgattcc agttgtcgag cgcggttcgg gcagattccg cccaatgcaa aatctcgctt
     9541 tcgttttggt tgaagtcgtg caattgctgc gcgtcataac cgaatatgcc gcttgtcagg
     9601 acaaagcgca gcgttccggc gcggcgcggt tcgagccaga agccgatgag tgcggaaagg
     9661 gcggcggctt cgggcgaggc gaacacggat tcgcgcgaaa gcaggacgct ttgcacctgc
     9721 cgttttttca gggcggcgga aaccatcacc gcctcgttgt gcgtgcgtac cagcacggca
     9781 atatcgcccg actgcaacgg gcagcctttg aaattcagac ggcctccggc ggcttcgttg
     9841 agcgcgtggg cgatttcgtc ggcgcaatag tcggcggcac gtcggcgcaa aacgtctttg
     9901 ttggcttttt cattgtcgtt ttcgtgcagc caacgaacct gtacggcagg acgtttgggc
     9961 gacaatctgc tttcggcacg cgccgcaccg acttccgaat agccgatgtt ttccaaaacg
    10021 aacgggcgtt ctttgaggcg gaacagcgcg cctatgctgc cgataagcgc ggcgtggctg
    10081 cggtagttgg tggcgagcgt gtagcggtgc cgcgcgtctt ccgccgcctg aaggtaggcg
    10141 taaatgtccg ctccgcgaaa gctgtaaatc gcctgtttgg gatcgccgac gaggaacagc
    10201 ggtcggtttt gggcgatgaa gattttttgg aagatttcgt attgcagcgg gtcggtgtct
    10261 tggaactcgt cgatcagcgc ggtttcccag ttttcggcaa cggcgcgggc gagggtttcg
    10321 gcgtgcggat tgtcggtcag cgcggtgtgg acatcgagca gcaggtcgtc gaaaccgcgt
    10381 tcgcggcgcg attttttcat ctcggcaagg ctgcggttga ggtattcgat taaatccagt
    10441 tgcagccgga tcattgttgc ttcttccgct tcttcgagtg cgttcaaatc gcgcccgaag
    10501 tctgccagtt tctgcaattc ggcaaatact gccgcatcgg gcgttttgcc ttttttcagt
    10561 ccggcttcga gtttgtcgga tgcaagtttc aagagtctgt cgtgtgtgtc tttgtccaga
    10621 aagggcagtt gtccggcggc ggatttttgt gccagttctt taaaaaggtt gccgaagctg
    10681 tttttgcggt aactgttgcc gttgaggtcg ggatgaatgc gccaaaagcc ggcttccagt
    10741 tctggcagca ggcggcagat ggtttgccat gaggtttcgg cgttgcgctg cgcctgtttc
    10801 aaatccgcct gcggacggcg gaaattcagg tacgggcggg aaagatagcg cgaaatttgg
    10861 gcaaggacgg tttgcggcac agctttgcgt ttaagcgcca atgcggcaag caccggatca
    10921 ttgctgacgc gttcccgcca aaaatcttgc gccgggataa gcaggcggtc gccgtcttct
    10981 tcggtcattt cgacatcgaa cggtgcttgg cacaggaagg cgtagtcgcg caggatgcgc
    11041 tggcagaagc cgtggatggt atagatggcg gcgttgtcga attgcccgat ggcggccttg
    11101 aggcggacaa tcagacgcgt ccggccctct ttttgcaaag cctgttttaa gagttcgggc
    11161 aggaaggtgt cgccttcgtg gtgttcggcg cagtaggcgg caatgccgtc tgaaagcgtg
    11221 tcgtctccaa gtttggcaat tcctttgctt tctaaaactt gtaacacatc gtccaaacgc
    11281 ccgcgcaggc gtgttttcag ctcggcggtg gcggctttgg tgaaggttac gaccaatacg
    11341 cgttcgacgt ttttttgttc taatacgatc aggcgtgtaa acagggcggc aatgccgtag
    11401 gttttgccgg tgccggcaga ggcctcaatc aggttggtgc cggaaatggg gacggttagc
    11461 gggtcgaatg cttggatgct tgcagacata gtgcgcgctc ggaaaacggt tggacg
//
LOCUS       DAWFBO010000059        11491 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFBO010000059 DAWFBO010000000
VERSION     DAWFBO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.059"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            271..1107
                     /locus_tag="WGO87_001083"
     CDS             271..1107
                     /locus_tag="WGO87_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0392452.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLDLRLINGVEISVTWRGRTIHVVGLDFDERDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
     gene            complement(1159..2451)
                     /locus_tag="WGO87_001084"
     CDS             complement(1159..2451)
                     /locus_tag="WGO87_001084"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0392453.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(2507..4414)
                     /gene="pglD"
                     /locus_tag="WGO87_001085"
     CDS             complement(2507..4414)
                     /gene="pglD"
                     /locus_tag="WGO87_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0392454.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFRILSTALAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEV
                     VKQMREYSAAFVDDDPKLWHTVICGLAVYRPDDISALIERYGVEKILLAIPSATQEQR
                     RRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSADI
                     EGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLNT
                     EILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLECA
                     LAATASGVRTFVLISTDKAVRPTNTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGNV
                     LGSSGSVVPLFEKQIAEGGPITLTHQDITRYFMTIPEAAQLVIQAGAMGTGGDVFVLD
                     MGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRKT
                     GHPRIMTANETMLPWRELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDLL
                     WVRETHRKNAV"
     gene            complement(4462..5637)
                     /locus_tag="WGO87_001086"
     CDS             complement(4462..5637)
                     /locus_tag="WGO87_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0392455.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKEHIEAVLAEATR"
     gene            complement(5715..6956)
                     /locus_tag="WGO87_001087"
     CDS             complement(5715..6956)
                     /locus_tag="WGO87_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ0392456.1"
                     /translation="MSKFFKRLFDIVASASGLIFLSPVFLILIYLIRKNLGSPVFFFQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDGERLTPFGKKLRAASLDELPELWNILK
                     GEMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNAASWDERFACDIWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPTFTGKRKLAVVGAGGHGKVVAELA
                     AALGTYGEIVFLDDRAQGSVNGFSVIGTTLLLENSLSPEQYDIAVAVGNNHIRRQIAE
                     KAAALGFALPVLIHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVTGNPAKPLAGKNTETLRS"
     gene            complement(7124..8541)
                     /locus_tag="WGO87_001088"
                     /pseudo
     CDS             complement(7124..8541)
                     /locus_tag="WGO87_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
     gene            complement(8736..9845)
                     /gene="ribD"
                     /locus_tag="WGO87_001089"
     CDS             complement(8736..9845)
                     /gene="ribD"
                     /locus_tag="WGO87_001089"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0392457.1"
                     /translation="MFSDTDISMMETALRLAALGRFSTSPNPRVGCVIAHGSQVVGQG
                     FHVKAGEPHAEVHALHQAGEMAQGATAFVTLEPCSHYGRTPPCAEALVRAGVSRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYRKHAHVRILMPSETAGGK
                     IDLNSLMRLLADEGIGELIVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLFSLP
                     ENRAALSAPPLWMPVSTEILGHDIKTVFRKNGNAF"
     gene            complement(9878..10342)
                     /gene="nrdR"
                     /locus_tag="WGO87_001090"
     CDS             complement(9878..10342)
                     /gene="nrdR"
                     /locus_tag="WGO87_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0392458.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            complement(10371..11213)
                     /locus_tag="WGO87_001091"
     CDS             complement(10371..11213)
                     /locus_tag="WGO87_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0392459.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT         2349 a         2947 c         3652 g         2543 t
ORIGIN      
        1 cacgttttca gacagtcttt tatagtagat taaaattgaa aacggtcata tcgccattcc
       61 ctcgaaagca ggaatccgga agtttgaaat agcggttaac cttaaacatt tccaataaat
      121 caaagtctgg attcccgcct gcgcgggaat gaccgcatag cggcttttcc tttgcatttg
      181 ccatacacct gttttttgaa atcaccgccg ccacaccgcc cgacaatatt gaaacaccct
      241 gcccaccatt taccacgcac ataacgcctt atgatagacc tgcactgcca ttccaccgtt
      301 tccgacggta tgctctcccc cgccgaagtc gtccgcctcg cgcatcaaaa cggctgcacg
      361 ctgctggcgt tgaccgacca cgaccacacc ggcggcatcg ccgaagcgcg tgccgaagcc
      421 gacaggctcg atttgcgcct gattaacggc gtggaaatct ccgtcacttg gcgcggacgc
      481 accatacacg ttgtcggttt ggatttcgac gaacgggacg aaaacctgca aaacctgttg
      541 gcgcaagtgc ggcaaggccg tctgaaacgc ctcgaagcca tcgccgccaa gcttgaaaag
      601 aaaggcatcg gcggcgcata cgacggcgcg ctcgcgctgg cggcaaacaa agaaatggtc
      661 agccgcacac acgtcgccga gttcctcatt cgggtgggac acgtcaaaaa caagcagcag
      721 gcgttcacca aatacttggg cgacggcaaa tcctgcgccg tccgccacga atgggcgacg
      781 ctggaagact gcgtctccgc cgtcaacggc gcgggcgggt tggcggtcat cgcccatccg
      841 atgcgttatg atttgtccgc caccgccaag cgcaatctgt ttgaagaatt taaaaacctc
      901 ggcggcgcag gcatcgaagt ccacagcggc aactgctgca aaaacgaccg cctcaactac
      961 gcgcttttgg cagaacgctt cggtatgctc gccagcgcgg gcagcgactt ccaccgttta
     1021 aacgacttta gcggcggcat tctcggcgcg tgtcccgaac tgccggaaaa ctgcaggccg
     1081 gtgtgggcgc atttttcccg atattgaatg cagataaaaa tgccgtctga aaacctttca
     1141 gacggcattt tttgcggttt aaacgttgtc gtacagtttt cggacggttt tgccgatggc
     1201 ggcgatgcct ttttccagcg tttgagcgtc ctgcgcgatg ctcatgcgga tgcactcgcc
     1261 cgcgtgcggg taatcccgcg tgtcgatgcc gacgaagaaa tgctcgcccg gaataatcag
     1321 cgtaccttcg gctttgagca tttcgtacag ggtttgcgaa gaaacgggca ggttttcaaa
     1381 ccagagccac aggaaaatcg cgccttcggg tttgtggatt ttcatcgggt acgcgcccag
     1441 ctcgcgcttg agcagcgaga cggcggtttg cgcctgattg cggtaaaacg gccggatgac
     1501 ttggtcggca agccgtttca tctcgccgct ttccagcagc ggggcggcga tggccgcgcc
     1561 gaagcgcgtg ggggcaaggt tcacaatcgc gttcaggctg ctgacggctt tgacgacttc
     1621 gggcgcggcg acgatgatgc cggtgcgaac gcccggcagg ccgactttgg aaaggctgaa
     1681 gcagaggatg atgttttcgt gccaattcag cgttacgtcg ctgtaaatga tgttggggaa
     1741 cggcattccg taggcgttgt cgataatcag cggaatcccg tgttcacgcg ccaaagcgtc
     1801 caaacgtgcc atttcgccgt cggtcaacac attgccggtc gggttggtcg ggcgcgaaca
     1861 gcaaatcgcg ccgattttgc ccgctttgag ttcgggcagg ttttccagtg cgtcaaagtc
     1921 cacgcggtat ttgaagaagc ctgcttcgcc ttcgtgttcg acgttttcga ttttgggctt
     1981 gacagaaacg aagtgctgcc cttcgatatg tacgtcggca tagccgatgt attcgggcgc
     2041 gagcggcaac aaaatggctt tttctgcgga tgtgccgtct gaaagcttga atttgccgcc
     2101 gaagaggttg aataaataga aaaacgcgtt ttgcgaaccg ttggtcagcg cgatattgtc
     2161 ggcggtcagg ttccagccgt attcgcggtt gaggaaggcg gtcaacgcgt caatcagcgc
     2221 ggcatcgcct tgggggttgg agtagttgcc gatgttttcg acggcgtgtt ctgccgccag
     2281 tttggagaat atgtcggcaa acgcccgatc gatttccgga atgcgcgccg ggttgccgcc
     2341 gccgagcatg ttgacgggct tgtcgctttt gagcgcgtcg ccgaggtcgt ccatcagttg
     2401 gaggatgccg ctgtgttgcg tgaatttttc gccgaatgcc gaaaactgca tgtcaaactc
     2461 cgtgtgtgta aggtaggttg gtttgtacgt tatgccgtct gaaggttcag acggcatttt
     2521 ttctgtgtgt ttctcgtacc caaagcaggt cgcagatacc gtcgctcggg gcaaagccgg
     2581 tcggggcgtt gatgagcagg gtgcggattg cctgctggtc gtaacggtcg caggccgcac
     2641 ggatgcggtc gagcagggcg gagagctcgc gccacggcag catggtctcg ttggcggtca
     2701 tgatgcgcgg atggccggtt ttgcggacgt tgtcgccgat gagcagctct tcgtagagtt
     2761 tttcgccggg acgcagtccg gtaatgagga tttcgatgtc gccgtcgggt tgttcgggtg
     2821 ttttgggttt gaggccgctt aaggtaatca tttggcgggc aaggtcgatg attttgacgg
     2881 attcgcccat atcgaggacg aatacgtcgc cgcccgtacc catcgcgccg gcttggataa
     2941 ccagttgcgc cgcttcggga atggtcatga agtagcgggt aatgtcttgg tgggtcaggg
     3001 taatcgggcc gccttctgca atctgttttt caaacagcgg gacaacggag ccggacgaac
     3061 ctaaaacatt gccgaaacgc accatgctga agcgggtttt ttgtccgggt tcggcggcga
     3121 gcgcttgaag gcagagttcc gccatgcgtt tgccggcacc catggtgttg gtggggcgga
     3181 cggctttgtc ggtggagatg aggacgaaag ttcttacgcc cgatgccgtg gcggcaagcg
     3241 cgcactcgag tgtgccgaag atgttgttgc gtatgccttc gacggtgttg aattcgacca
     3301 tggggacgtg tttgtaggcg gcggcgtgat agacggtcgc aacggaaaag gcggtcatga
     3361 cgtgttcgag cagggtgcgg ttttgcaccg aaccgagaaa gggcaggatt tcggtgttga
     3421 ggcgtttttg gatgcaggtt tcgcgcaatt ctttttcgat ggcgtacagg gcgaattcgg
     3481 ataactcgaa cagcagcagc ttttcggggc ggcggcggat aatctggcgg cagagttccg
     3541 aaccgatgga gccgcccgcg ccggttacca tgacggtttt gccttcgatg tcggcactca
     3601 tgaggcggtt gtcgggcgcg acggaatcac gcccgagcag gtcggacacg gagatttttt
     3661 tgagcgtgcc gatgctgatt ttcccgtcca tcaggtcttt cattccggga atggtcaaca
     3721 cttcgcaggg ataggcttcc agtttgttga tgattcggcg gcgttgttcc tgagtcgcgc
     3781 tgggaatggc aagcaggatt ttttccacgc cgtagcgttc gatgagggcg gagatgtcgt
     3841 cggggcggta aacggcaagg ccgcagatga cggtgtgcca cagtttgggg tcgtcgtcca
     3901 caaaggcggc ggaatattcg cgcatttgtt tgacgacctc gagaagctgt ctgcccgaac
     3961 gccccgcgcc gtaaataatg acggggatca tctgtttttt ggggtgttcg gacaacagtc
     4021 cgcgcaaaac catacgcgag ccggtcacgg aaacaaacag cagtaagaaa tagacaatcg
     4081 gcagggcgag gcgcagcctt tcttcaaaaa tcaatgtgtt gaggaagaac aggacggcgg
     4141 aaacaatgct gcctgccagc gcggtggaga ggatgcggaa gctgacgaag cgtgtaacgg
     4201 cgcggtaaag ccccattcgg ataaataatg tgatggtcag caaggcagtc agcaaaaaag
     4261 actgccagtt ggcaaaatcg aaccattcgt ccgagtagtc ggcctttagg ctttgggtga
     4321 accaaaaggc aatgaaaatc atcagaaaat cgtgtatgag gaaacagatt ttcttgatgt
     4381 tgcgcggcag ggcgatcagg gtttccagag tcatgttgtc ggcggcgtgt atttttcaga
     4441 cggcatatgc cgtctgaatg gttatcgtgt ggcttctgcc aatacggctt cgatgtgttc
     4501 tttgcagaac gcgatttcgt cgtcggtcag cgtcgggtgc accaagaaca tcaggctggt
     4561 gtcgcccagt tcgacagcat ttttcaaacg ctctttcggc cgccaaggcg tgtcgtcgaa
     4621 agatttttcc aaatagactt cggagcagct gccttgatag caggggactt tgcgcgcgtt
     4681 cagttcgctg acgatgcggt cgcgcgtcca gccgtctttg aggtgttcgg gtttgacgaa
     4741 agcgtagaac ttatattgcg cgtgtccgat gtagtcggcg acttcaacca agcggatact
     4801 tttgaatttg cctaggcttt ccgccagctt ggcggcgtgc gcttgacggc aggcagtcca
     4861 ttcgggcagg cgtttgagct ggatgcgccc gattgccgcc tgcatttcca tcatacgcca
     4921 gtttgtgccg aaactttcgt gcagccagcg gaaaccgggc gcgtgttcgc ggtggtacac
     4981 ggcatcgtag cttttgccgt ggtctttgta cgaccacatt ttttcccaca gggttttgtc
     5041 gttggtcgta accatgccgc cttcgccgcc cgtggtcatg attttgtctt ggcagaacga
     5101 ccatgcgccg acgtgtccga tgctgccgac ggatttgcct ttgtatttcg cgccgtgcgc
     5161 ttgggcgcag tcttcgatga cccaaagatt atgttctttt gccaaagcca taatgccgtc
     5221 catttcggcg ggcataccgg cgaggtggac gacgatgacg gctttggtag tcggtgtcag
     5281 cgcggctttg acggtttccg cgctgatgtt ttggctgttc aaatccacat cggcaaacac
     5341 cggatttgcg cccgcgttca caatgcagga cgcggaagcg aggaaggtgc gcgaggtaac
     5401 aatcacatcg tcgcccgcgc ctatgcccat tgctttgagc gcgacatcga gtgccagcgt
     5461 gccgttggca agggcgacgg cgtaccgcgt gccggcaaag gcggcaaatt ctttttcaaa
     5521 ttcgcggcat tcgttgcccg tccagtagtt gactttgttg gacagcagga ctttggaaac
     5581 ggcatcggct tcttcttggg tgaagcaggg ccacggggaa aggaaagtgt tcagcatgat
     5641 ggtttgtccg tcggttttca gacggcattt cctcatttct gccgtctgtt gcttaggctg
     5701 tttattttaa tcgtttacga ccgcagggtc tcggtatttt tgcctgccaa tggctttgcc
     5761 ggattgcccg tgacggtcat gccgtctgaa acgtcgcgca cgacgactgc gcccgctcca
     5821 atggttgcgc ggctgccgat acggatctgc tggcggctgc acgcgcctgt gcctatccag
     5881 ctttcttcgc cgatacgcgt gttgcccgac aggtgcgcgc ccgggctgat gtggacgaaa
     5941 gcatcaagca ggcagtcgtg atcgacggtg gcggcggtgt tcacaatcac gccgtctttc
     6001 aatacgctgc cagcctgtac gacggctttc gccataacga cgctgccttg tccgactgtt
     6061 gcagaaggcg agacggtcgc gtcgggatga atcagaacgg gtagggcgaa gccgagcgcg
     6121 gcggcttttt cggcgatttg gcggcggatg tggttgttgc cgacggcgac ggcgatgtcg
     6181 tattgttcgg gcgataaact gttttcaagt agcagtgtcg tgccgatgac ggaaaagccg
     6241 ttgacgctgc cttgggcgcg gtcgtccaga aaaacgattt cgccgtatgt gccgagtgcg
     6301 gcggcaagct cggcaacgac tttgccgtgt ccgcccgcgc cgacgacggc aagtttgcgt
     6361 tttcctgtga aagtgggcat ggtagcttcg ccctgtgctg aaatcccttc cttgattaat
     6421 acttttttga ctgtcagcag taggattttg atgtcgaggc acaggctgaa gtggtcgata
     6481 taccagatgt cgcatgcgaa gcgttcgtcc catgaggcgg cgttgcgccc gttgacctgc
     6541 gcccagccgg taatgcccgg tttcatttcg tggcggcggt tttggaagtt gtcgtacagc
     6601 ggcagatatt gcatcagcag cgggcgcggg ccgaccaggc tcatctcgcc ttttaagata
     6661 ttccataatt caggcagttc gtccaaactg gcggcacgca gttttttgcc gaacggtgtc
     6721 aggcgttctc cgtcgggcag cggaatgccg tctgaatcaa gcgcgtcgcg catggaacgg
     6781 aatttgacca ttttaaaagg ttttccgtcc tttccggggc gttcctgaaa gaagaagacg
     6841 ggcgaaccta gattcttgcg gatgaggtat atcaaaatca aaaatactgg cgagaggaaa
     6901 atcagtcccg aggcggaggc aacaatgtca aacaggcgtt tgaagaattt actcatgggc
     6961 taatctttca atcaggttga cgattttccg ataggaaatg tcgcgcctga agcggcggac
     7021 gatttcgtcg gactgaacgg ggtcgttttt gcacgccatt tggcaatcga tgatatcgtg
     7081 cccgtgccgc ccaagcctgc catagatgtg gtcaaaacga ttttcataat gggaaacctt
     7141 gttttttcaa ataatgaaac agtttgtgca aatctttccg gtggcgcagg atgcagcctg
     7201 ccagatatgc cgcccatatg ccggcaaaca gggggtagtt tgccggcgtg ccgaagcagg
     7261 tgtaggccgc cgaggaggcc aggcagaaca atgtgtgcag ataaagcggc aggcgtttga
     7321 gcggctgcca caggcggcag gagctttcgg tcttgaaggc aaaaaacagc cagaatgagg
     7381 cggcacaggc aaccgccgcg ccgcgcgcgc cgccggacgg tacggcaagc cccagcagca
     7441 gcaggtttgc cgccagcgcg cccaaggtgg cgagcgcgat cgggcgtgtt tttcggacga
     7501 cgttcaaacc gatgccgctg atttctacca gcgtgcaaaa cagcggcggc agcatacacg
     7561 atacgacgat aaaccggacg gcggcgtagt tttccggcag caggagggag gcgaggggcg
     7621 agaaaatgcc ggtcaggcag acggcggcgg caagcagggc ggaggcagat tctgccgttg
     7681 ccgagaggcg ggcggggtgt ttgcttcgat tgcgcggaaa atatacggtg tccagaccgt
     7741 tgaaaagatg ctttggaaca ataatgccgc cccgccgaac gaaataccca tcgaataaac
     7801 gccgagctgt tctaggccgg catatttttt caggaacaaa cggtcggcgg atgccagccc
     7861 ccaataggcg atgctgctta gtgcgatcgg tatgccgtag cgcaggccgc gatgcaggac
     7921 ggcggatgaa aacggtgcgc gccggacggc cttcagacgg catcggtttt gaaacagcaa
     7981 aaaggcggcg gcggcaaggt ttgccagcgc gtaaacggcg gtcaggacgg cggtgttcgc
     8041 cggaaagtgc agcagcccga ccgtcagcgg cagcagcagc aggatggcga gcttggacac
     8101 gagttgcgcg gacgaaaagg caagggcgcg cccttccata cgcaaaacca gtaagagaaa
     8161 gcggatgggc aggaagctca gttcaaacag caccagcccg atgccggcgg cggcatcgtc
     8221 gagcgaaaac aggatttcag acggcaggga cgggcgggaa agcagcaggg cggctatcgc
     8281 ggcggcagac agcagcggcg gcaggaacag ggttttgaac aaggtgtctt tgtcggcggc
     8341 ggcatagtat tcgcggacgt atgcctgatc cagcccgagg cacaataccg acacagtcag
     8401 tcccgccgcc gtctgcatca gcacgatgcg cccgatgtcg tcggcgggga aataccacga
     8461 cagcagcggc aggatgatga cggctaaaac cgcgctgccg atcgagcctg ccgcgtagcc
     8521 gaggatttct tttgtgtcca tttttgatgt ccgggcggcg gcgggatgct gcctgtgtcg
     8581 tctgaagcct ttcttgatcg gaatttgacg gctttcagac cgtcgcggct gccggcgggg
     8641 tgcggcagtc cgggtcgcgc ttttccgggc gggcggcggt ctgaacgggg ccgtttttta
     8701 tcggcgttat tatatagtga aacggcgcaa accttttaaa aggcgttacc gttttttcgg
     8761 aacacggttt tgatgtcgtg tccgaggatt tcggtggaaa cgggcatcca caagggcggt
     8821 gcggaaaggg cggcgcggtt ttcgggcagg gaaaacaggt ctttgccgct gccgaggatt
     8881 ttgggcgaac ggtacagcac gatttcgtct gccaaatctt ctgccaaaaa tgcagatgtg
     8941 agttcggagc ctgcttcgac tatgagttcc cctatgcctt cgtcggcaag caggcgcatc
     9001 aggctgttca aatcgatttt gccgcctgcc gtttcagacg gcatcaggat gcggacgtgg
     9061 gcgtgtttgc gataggggcg gagtttgtct tcatcgcgtt ccaaggtggc gatgtaggtt
     9121 ggagattgtc cgtcggtcag aaggtggctg tccaagggca ggcgcaggcg gctgtctaaa
     9181 acgatgcgtg cgggttggcg caaagttgga aaagcgcgga cgttgagccg gggattgtcc
     9241 gccaacaccg tgccgatgcc ggtcagcacc gcgcagcttt cggcacgcaa aacctgtacg
     9301 tcggcacgcg cgtcttcgcc ggtaatccaa aagctgctgc cgtctgaaag ggcggttttg
     9361 ccgtccagcg aaacggcgca tttgaggcgg acaaaggggc ggcggcgttc gatgcgcgac
     9421 aggaagcctc ggttgagttc ccttgcctga tgttcgagta aaccgcattc cgtcttgatg
     9481 cctgctgctt cgagcagggc aagccctttg cctgcaacca gcgggttggg gtcgcgcatg
     9541 gcggcaacga cgcgggacac gcccgcccgc accagtgctt cggcgcaggg cggtgtgcgt
     9601 ccgtaatggc tgcatggttc gagggtaaca aaggcggtcg cgccttgagc catttcgccc
     9661 gcctgatgca gggcgtggac ttcggcgtgc ggttcgcccg ctttgacgtg gaagccctgc
     9721 ccgacaactt ggctgccgtg tgcgataacg cagccgacgc gcggattggg cgaagtggaa
     9781 aaacgcccca aagcggcaag tcggagggcg gtttccatca tggatatatc tgtgtccgaa
     9841 aacatgggga taccgtatca gtatgggttg gggagaatca ggctttgcgg tctgttttga
     9901 cggcttgcgc cagccacgag gcaaagtctg ccggattgtc gaagcgtttg tgcagggcgg
     9961 cgaaacggac ggcggcttcc gtatcttgtc ggagcagttc ttttagcacc atgtcggcaa
    10021 gtgcggcaga ggggatgtcg cgctgacccg aagtgtagag cctgtgttcc gtcaggcgga
    10081 cggtttcgtc gatctgttcg ggtgtcaggg cggattttcg ggcggcggcg gtcaggtcgt
    10141 tgcggaggcg ttgtgcatta aagggcgagc gttttttgtc cggaccgatg acggcgggca
    10201 ttttgagttc ggcggtttcg agcgtgccga agcgtttgcc gcagctgggg cagtggcggc
    10261 ggcggcgcac ggcgttgcgt tcttccatca ggcgcgaatc ggcgacgcgg gtgtcggggt
    10321 gggcgcaaaa cgggcatttc atagcgtttc ttcctgatgc cgtctgaacg tcaaaccgat
    10381 acgccggcgg cgcgggcgat ttccagacct tcttcggcac tcatatagac ggggttttcg
    10441 ggacggtcgt gtcggacgat gttgccttcg cggaacatga ccagttcgtc cacggcaagt
    10501 tgggaccatg attcgtcgcg ggtcagcggc agggtggcga taacggcgac gcggtcggag
    10561 ggcgtggtta cttcggcaaa atcgaccatc acgtcgtcgt cgagcaggcg cgccttgccg
    10621 aacggggctt ggcggacgat gtagtgcagc agtgtgctgg cgtgggcaaa cagggcaatg
    10681 ccgtctgaaa gcataaagtt aaacagcccg aacttgcgga tttcgtgcgt cagccccgca
    10741 atcgcgtcaa acagcgtgtc gtcgtcggga cgggcggcaa agcgggtgcg caggcggttg
    10801 aggatgtggc agaacgcgcg ttcggaatcg gttgtgccga cggggtggaa aaattcgccc
    10861 tgttcgggaa aaaaatcaat caaatgtccg ttgtgggcaa acagccagta gccgccccac
    10921 atttcgcgca taaagggatg ggtgttcgcc agcgaggttt gtccttgtga tgctttgcgg
    10981 atatgggcga cgacgttttc cgatttaatc tggtaggcgc gcacgaggtc ggcgacgggc
    11041 gaattggcac tgggtttgtc gtcatggaac aggcgcacgc ctttgccttc gaaaaagccg
    11101 ataccgaaac cgtcggcgtg atggtcggta atgccgccgc gccggcggaa gccttcaaag
    11161 gaaaacataa tatcggtcgg cgtattgcag ttcatgccca gcagttgaca catggtttgt
    11221 ccaaatgatt cagatggtcg caagtattcg gattataccc cgaactgaaa atgccgtctg
    11281 aaatacggct tgttccccat tattccctcg aaaacagaaa acaaagacgg aaacttaaga
    11341 ttccgtcatt cccgcgcagg cgggaatcta gaatttcaat gtctcaagaa tttatcggaa
    11401 aaaaccaaaa cccttccgtc gtcattccca cgaaagtggg aatctagaaa tgaaaagcaa
    11461 cgggaattta tcggaaacga ccgaaaccga a
//
LOCUS       DAWFBO010000060        11059 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFBO010000060 DAWFBO010000000
VERSION     DAWFBO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11059
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.060"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1..405)
                     /locus_tag="WGO87_001092"
                     /pseudo
     CDS             complement(1..405)
                     /locus_tag="WGO87_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(638..2695)
                     /gene="ppk1"
                     /locus_tag="WGO87_001093"
     CDS             complement(638..2695)
                     /gene="ppk1"
                     /locus_tag="WGO87_001093"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0392460.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2864..3307)
                     /locus_tag="WGO87_001094"
     CDS             complement(2864..3307)
                     /locus_tag="WGO87_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0392461.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAINAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(3314..3829)
                     /locus_tag="WGO87_001095"
     CDS             complement(3314..3829)
                     /locus_tag="WGO87_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0392462.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            4099..6729
                     /locus_tag="WGO87_001096"
     CDS             4099..6729
                     /locus_tag="WGO87_001096"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0392463.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVIAVGDNAFDANQWQEWYGDKENGV
                     LVNSGDLDGMNFQTAFDAIAAKLQSQDAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVSVPADQLPVVLPENVVPDGMGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            6964..7776
                     /locus_tag="WGO87_001097"
     CDS             6964..7776
                     /locus_tag="WGO87_001097"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEZ0392464.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEQYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKEGFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            7769..8578
                     /locus_tag="WGO87_001098"
     CDS             7769..8578
                     /locus_tag="WGO87_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0392465.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFADPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            8575..9129
                     /locus_tag="WGO87_001099"
     CDS             8575..9129
                     /locus_tag="WGO87_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392466.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRTLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYSINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            9781..10035
                     /locus_tag="WGO87_001100"
     CDS             9781..10035
                     /locus_tag="WGO87_001100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392467.1"
                     /translation="MPRGRGCFGFHARLRRALLSGYAVCTAAVSAWFDAVGHLCGYAL
                     IFSSAVLPVLAGIFSSASIVNLLILPFAKSATQWLRYPCF"
     gene            complement(10004..10315)
                     /locus_tag="WGO87_001101"
     CDS             complement(10004..10315)
                     /locus_tag="WGO87_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0392468.1"
                     /translation="MKYKSEIFAAIHETMQGLHDIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(10312..10680)
                     /locus_tag="WGO87_001102"
     CDS             complement(10312..10680)
                     /locus_tag="WGO87_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0392469.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLIFFRYGERAFFMTAFAKNDRENITDKELAELKKAAAIILA
                     MTETDIEQAKSNGTFTEIQP"
BASE COUNT         2670 a         2808 c         2859 g         2722 t
ORIGIN      
        1 tgctcagact atcggtataa acaatactgt ccggcatgat tttctttttg atgacaggga
       61 gtaacgtttc agacttggca ttatccacca caacggtata gacccgtccg ttgcgtttca
      121 gaatgccgaa gacaaccact tttcctgccg cccgcgacca cgtctgcctt tacgccgtcc
      181 gccgaaatcg ctttcgtccg gctcgacagg gccctcaaaa acctcatcgg cagccaaggc
      241 caaatgatgg ttgataaccg tgcggatttt acggtagaac agtgctgccg aattgggatg
      301 gatacccaaa atatcggcgg cagaacgggc ggtaacttcg agtacaaaaa aacggagcag
      361 ttctttctgt acttttttct ttcatttgca gtgcgttatc ttcatatttc gagggtaaca
      421 tatctgctaa tctagtacag cccctaaaat ttaatccact ataatgccgt ctggaatgcg
      481 gcggattggg gttgggagcg tataggggaa gtgcttatgc ggaaatggcg aaggtgtgtt
      541 tttgactccg gtccgccctg tttagattgc tgtaaaacca tgccgtctga aacggaaagg
      601 tttcagacgg catggttttt gattggggcg gtgcgggtca gcctccgagc agtgtccaca
      661 aatcgttttg caggtcggct tcggattcgc cctcggcagg tgcggcgcgg atatagccgc
      721 cgtcgggctg catcagccac gcgtgcgtat tgtctgccaa tgccatttcc aaaccttcgc
      781 ggatgacgcg ttttttgagt tcgggcgcgg taatcggcgc ggcggtttcg atgcggcgga
      841 agaagttgcg ccccatccaa tccgcgctgg agataaaggt atcgtccgcg ccgttgttgt
      901 ggaagcaata cacgcgcgcg tgttcgagct gcctgccgat gatggagcgg acgcggatgt
      961 tttcggacaa gccttttaca cccgggcgca aggtgcacat accgcgcaca atcaaatcga
     1021 tttgtacgcc tgccgcgctt gcccgataca gggcttcgat gacggtcggt tcgatgagcg
     1081 aattcatctt ggcggtaatc cgcgccggtt tgccggcttt tgcgtgttcg gtttcgcgtg
     1141 cgatgcggtc gataaccatt ttgtgcaggg taaacgggct ttggtagagt ttgttcagcc
     1201 gcccgggttt gcccaagcct gtgatttcca taaacaatgt gttcacatcg gcggtgattt
     1261 gttcgtcggc ggtaatgatg ccgaagtcgg tgtagatgcg cgatgtgcct tggtggtagt
     1321 tacccgtgcc gaggtgggcg taacgcttga gtacgccgtc ttcgcggcgg atgaccagtg
     1381 ccatcttggc gtggactttg tagccgaaca cgccgtacac gacgtgcgcg cccgcctctt
     1441 cgagctgctt cgcccagttg acgttgttgg cttcgtcaaa acgcgccatc agttcgacga
     1501 cgacggttac ttgtttgccg gcgagtgccg ccttcatcag ggcgcggacg agttcggaac
     1561 gcgtgccggt gcggtaaatc gtcattttga cggcaagcac gtcggggtcg gcggcagctt
     1621 cgcgcatcat ttcgacaacg gggtcgaacg attggtaggg gtggtgcagc aggatgggcg
     1681 attggcgcac caaatcgaat atggacgcgg ttttgcccaa ggctttcagt ctgcccggcg
     1741 tgtgtgtggg aaatttcaaa tcggggcggt tgactaggtc ggggacggcg ttgaggcgca
     1801 cgaggttgac cgggcctttg acctgataga gttcggcggc ggtcagtttg aattgcgcga
     1861 gcagaaagtc gcggatgtag gcgggacagg tgtcggcgac ttcgagccgc acgccgtcgc
     1921 cgtattcgcg gtcgtgcagt tcgttttgga tggcggcgcg gaggttttgc aggtcttctt
     1981 cgtcaacggt caagtcgctg tcgcgcgtca ggcggaactg gtggcagcct ttgacgttca
     2041 tgcccgggaa gagtttgccg acgtgggcgt gcaggatgga ggagaggaag acgaagccgt
     2101 gtccgccgcc acacagttcg gacggcaggg gaacaacgcg cggcaggatg cgcggtgcct
     2161 gcacaatcgc catccccgaa ggcctgccga acgcgtccgt gccgtcgagt tcgacggcga
     2221 agttgagcga tttgttcagc ggtcgcggga aggggtggga cgggtcgagt ccgatggggg
     2281 tcaggatcgg cagcagttcg cggtcgaaat agtcttcgat ccattttttc tgcgcgcccg
     2341 tccagttgcg gcggcggtaa aaatggatgc tttcccgcgc caactcgggc tgaaggacgt
     2401 tgttgaacag gtcgtactgg tgccgtatca gggagcgcgc cgcttcggta acgtcggcga
     2461 tggtttcaga cggcattttg ccgttgtcca gcctgcgccg ggggtgcagc ttgttttcac
     2521 gcttgagcca cgccatgcgg acttcgaaaa actcgtcaag attggacgac acgatgcaca
     2581 ggaagcgcag gcgttccaaa agggggacgt ttttgtcttc cgcctgtgcc aacacgcggc
     2641 ggttgaatgc cagcaggctt agttcgcggc agaggatgcg gttttgttcg ggcataaggt
     2701 tctcccaaag atggttgttg ttcggtcgga gcgggcaggc aatgccgtct gaaggcttgg
     2761 cggcatctgc gccggtttca gacggcatga cgtggcggac agcctgtttc aggcatcggg
     2821 gcaatgtgaa aacagatacc ggcgcgtgcg gtatccgtct gtttcaaatc acttccaaca
     2881 caaaataatg acgcagtttt tcgtacaccg catcgctgac gttgatgcag ccgttggtca
     2941 taatcctgtc ggacacggac ggggaggcga tgcgttcgtt ccgcctttcc gacggtatct
     3001 gattccaaac gcggtgcagg gcgaaaagaa aatcaccctc ctgcttgaaa ccgatgactt
     3061 cgccgccgta tccaggcttg tcggtactgt tcagcgtcag cccgaacgtc cccttgggcg
     3121 tggcaacacc gatgaggacg gggtggcact gacggtcgtc agcgaaacag agttccgctt
     3181 tggatgtatc gacgatgact tttttctttt ggatataggc attgatcgca tccggttgcc
     3241 cctgtgcata tacgggggca accattgaca ggccgcataa tatccctaaa aacaggcgga
     3301 acggcatgtc ggattattgg cggatacgtt tcggttttgc cggcacgatt tcgcggatga
     3361 tgacttgcgg ctcggctttg ggtgcgggcg gcggacaggc ggcatctttc gggaagacgg
     3421 ggttccagta gaagctgcgg gcaaatttgt ctttatcgaa aatcactttg tattggcagg
     3481 tagtaacatc ttctacgccg gaagtgtttt cagggtcgat acctacgccc ggggtatgga
     3541 agtggaacag gtaatcccat tcgcgcacgc cgtacatacc ttcgtcgtaa tgcgggcggc
     3601 ccaggatttt gtagatgtcg tctttggtca ggccggggcg catctgatcc agttcgtcat
     3661 aagtcgggaa tgtgccgcgc ttgttgtcga gcgttacgga atagggtttc gggaaaaccg
     3721 gattgtcggt cgtgccgtcg gctttgacgt tgcttttggt tgcgcaggca gacagaacgc
     3781 ccgccgccaa tactgccaag cccggtttga cgatttgttt gattttcata tgcagaatcc
     3841 tcttttaccc gatgcccgtc tgccttttgt tcggaagagc cgcatcagga gagttttaaa
     3901 catttgattt tggtttgtaa tattagcata aaaaacgtgc gtatcaataa acgcggtgta
     3961 tttgtacggc atacggaata atgcgcgtgc gaatttacgc atcctgccgg caatctgccg
     4021 attcgccgct tccggcaacc tgttataatt gatgccgact ttatttttcc aacgttttta
     4081 agcgaaaaca gaatgactat gcaagaacaa tatcaacccg ccgccatcga gcctgcggcg
     4141 cagaaaaaat gggacgacgc ccgtattttc aacgtctccg aagacgcttc caaacccaaa
     4201 tattattgcc tctccatgtt cccctatccc agcggcaagc tgcacatggg gcatgtgcgc
     4261 aactacacca tcggcgatgt attgagccgc ttcaaacttt taaacggctt caacgtcatg
     4321 cagcctatgg gttgggacgc gttcggcatg ccggcggaaa acgcggcgat gaaaaacaac
     4381 gtcgcccctg ccgcttggac gtacgataac atcgaataca tgaaaaccca gctcaaaagc
     4441 ctgggttttg cgattgactg ggcgcgcgaa accgcaacct gcaaacccga atactaccgc
     4501 tgggaacaat ggctgtttac caagctgttt gaaaaaggca tcgtttatcg caaaaacggc
     4561 acggtaaact gggacccggt cgaccaaacc gtccttgcca acgagcaagt tatcgacgga
     4621 cgcggttggc gttcgggcgc gttgatcgaa aaacgcgaaa tcccgatgta ttacttcaaa
     4681 atcaccgatt acgccgaaga gctgctcaac gacttggaca agctggaaca ctggcctgaa
     4741 caagtcaaaa ccatgcagcg caactggatc ggcaaatctc gcggcatgac cgtgcgcttc
     4801 gccgtttcag acgacagcaa acaaggcttg gaaggcgatt acgcgaaatt cctgcaagtt
     4861 tataccaccc gccccgacac gctgatgggc gcgacttatg ttgccgttgc cgccgagcat
     4921 ccgctggcaa ccgcagccgc cgccgacaaa cccgaattgc aggcatttat cgccgaatgc
     4981 aaagccggtt cggttgccga agccgatatg gcgacgatgg agaaaaaagg cgtgccgacc
     5041 ggtcgctacg tcgtcaaccc gctcaacggc gataagctgg aagtgtggat tgccaactat
     5101 gtattgtggg gctacggcga cggcgcggta atggcggttc cggcgcacga cgaacgcgat
     5161 ttcgagttcg ccgccaaata cagcctgccg aaaaaacagg tcattgccgt cggcgacaac
     5221 gcatttgacg caaaccaatg gcaagagtgg tacggcgaca aagaaaacgg cgtattggtc
     5281 aatagcggcg acttggacgg tatgaatttt cagacggcat tcgacgccat cgccgccaag
     5341 ctgcaaagcc aagacgcggg cgaaccgaaa acccaatacc gcctgcgcga ctggggtatt
     5401 tcccgtcaac gctactgggg ctgcccgatt cccatcgtcc attgcgaaaa atgcggcgac
     5461 gtatccgtcc ccgccgacca actgcccgtc gtcctgcctg aaaacgtcgt acccgacggc
     5521 atgggttcgc cgctggcaaa aatgcccgag ttttacgaaa ctacctgccc atgctgcggc
     5581 ggcgcggcga aacgcgaaac cgacaccatg gacaccttca tggaatcgag ctggtatttc
     5641 ttccgctata tgtcgcccaa gttttcagac ggcatggtat cggctgaagc cgcaaaatac
     5701 tggggcgcgg tcgaccaata catcggcggc atcgaacacg cgattttgca cctcttgtac
     5761 gcgcgtttct tcaccaaact gatgcgcgac gaaggtctag tcaatgttga cgaaccgttt
     5821 gaacgcctgc tgacgcaagg catggtcgtt tgcgaaacct actaccgcga aaacgacaaa
     5881 ggcggcaaag attggatcaa ccccgccgat gtcgagctga cgttcgacga caaaggccgc
     5941 cccgtttccg ccgtcctcaa agccgacgga ctgcccgtcg tcatcagcgg cacggaaaaa
     6001 atgtccaagt ccaaaaacaa cggcgtcgat ccgcaagagc tgattaacgc ctacggcgcg
     6061 gacaccgccc gcctgttcat gatgttcgcc gcaccgcccg aacagtccct cgaatggagc
     6121 gacagcggcg tcgaaggcgc gcaccgcttc ctgcgtcgct tgtggcgtac cgtttacgaa
     6181 tacctgaaac aaggcgaagc agtcaaagca tttgcaggca gccaagacgg tttgtctaaa
     6241 gaactcaaag acctgcgcca caaactgcac gcgaccactg ccaaagtcag cgacgactac
     6301 ggccgccgcc agcagttcaa caccgccatc gccgccgtga tggaattgct caaccaatac
     6361 gacaaaaccg acaccggcgg cgaacaagga cgcgccgtcg cccaagaagt attggaaacc
     6421 gccgtacgcc tgttgtggcc catcgtgccg cacatctgcg aaaccctgtg gagcgaattg
     6481 aacggcgcga aactgtggga agcaggctgg ccgacagtcg acgaagccgc cttggtcaaa
     6541 tccgaaatcg aagtcatggt tcaagtcaac ggcaaactgc gcggaaaaat caccgtcgcc
     6601 gccgacgcat ccaaagccga cctcgaagcc gccgcactcg ccaccgaggg cgcagtgaaa
     6661 ttcatggaag gcaagcccgc caagaaaatc atcgtcgtgc cgggcagact ggtgaacatc
     6721 gtcgtctaac cgcttttaag gtttatccac acggataaag gccgtctgaa acttggaaac
     6781 agggtttcag acggcctttt taaggcagat ggagttgata agtccaataa catattggaa
     6841 tggaaaggtt ctattggatt tagactcact ctacacacgc ccccaaatat aaatccattg
     6901 tattcctcac gatgttttgg cacaattcca agccaatcaa ccacgcttgt actcggcatc
     6961 ctaatggctt caaaaccttt tctaaaatgg gcaggcggca aacataaact tgtccccttt
     7021 atcgaacaca atctacctac tcccacacga aaacgcctga ttgaaccctt ttgcggttct
     7081 gccgctttgt ctcttgcatt ggatttcgaa caatatctgc tcaatgatat taacgctgac
     7141 ttaatcggct tattccgtat cctaaaagaa gaaaaatccg gattcatcga ctacgcccgc
     7201 tctttcttta tttccgaaaa caatagcgat tcaagattct atgaattgcg ggaacaattc
     7261 aacttcagca aaaatctgca cgaacgctcg gcactattta tttatttgaa ccgtcatgct
     7321 tttaacggtt tatgccgtta caacagcaaa ggggtattca atgtgccgtt cggacgctac
     7381 aaatcgccgt actttcccca acaagaaatg gaaggtttca ttcagaaatc tgacagagtt
     7441 gaattaatgt gcggagattt tcaaatgatt ctatctcaga cagacaatac cgatacggtt
     7501 tattgcgatc ccccttacgc gcctttaagc ccaaccgctt catttaccac ttatgccaaa
     7561 gaaggtttca atttagacaa tcaaatccgt cttgcccagt cagcccaaca aattgcgccc
     7621 gattcccaag gcgttttgat ttcaaatcat gatactgaat tcacaaggga tatttattct
     7681 tttgctattc tgaaaacaat tgaagtacaa cgcaatattg ccgcaaaagg cagcagccgt
     7741 aaaaaagtcg gagaattatt ggcaatttat gattgatttt gacaaaccgg ctgaagaaga
     7801 cgccatttat caaagtcgtt tgaaaaaaat cgtttcagac gaccttgtcc tctacaacga
     7861 aaattccctt aacgtcatgc ggaagatatt ggaaaaatat ccaaacggct gttttgatat
     7921 gattttcgca gacccgcctt actttctttc caatgacggc ttcagttgtc aaaacggaca
     7981 aatggtttcc gtcaataagg gcaactggga taaatccaaa ggaatggcgg cagatttgga
     8041 attctacgaa gaatggctcc gactgtgtta cgccttatta aaaccaaacg gcacaatttg
     8101 ggtttgcggc acattccata atatttattt aatcggctac ctgatgcaaa ctatcggata
     8161 ccatattttg aacaatatta cttgggaaaa acccaatcct ccccctaatt tgtcctgccg
     8221 tttttttacc cattcgacag aaacaatctt atgggcaaag aaaaataaaa aagccaaaca
     8281 cacgtttcat tatgaaatga tgaaagcaca aaatgatggc aaacagatga aatgtgtttg
     8341 gacatttcct cctccaaaca aagccgaaaa aacattcggc aaacatccga cacaaaaacc
     8401 actccccttg cttgaacgct gcatactctc agcttcaaac atcggagatt taatttttga
     8461 ccctttcatg ggcagcggca caacaggcgt tgccgcctta aaacatggtc ggaaattttg
     8521 tggttgcgaa ctggaagaag atttttttga attagcaaaa aaaaggttag aaaaatgatt
     8581 attggcggaa tcggtggtgc aaggacacaa acaggactca gattcgaaga acgtacagac
     8641 ttacgaacgt tgtttgaaga aattcccggg tacgacttaa gaaaaacata tgataatgcg
     8701 ggttatgaag tttggttcaa tggagaattg aaggcttatt gttttaaaaa atatgagttt
     8761 taccgatttt tgaaaagacc gggatacagt attaattgga aagaccatct gtctaaaaga
     8821 ttactacccg acaatggctt atttatcatc atccgtgata ccttatttat tattgagatc
     8881 aaattccaac aaactcccgg ttcagtagat gagaaattac aaacttgcga ttttaagaga
     8941 aaacaatata caaaattggt tcactcttta ggttggcggg ttgaatatgt ctatgttttg
     9001 aatgattggt ttacaaagcc ggaatataaa gatgttttag attatattat cagtgttaac
     9061 tgccattacc aatttaacac gattccctta aggtggtttg gactgcctga tggtgaaaca
     9121 aatgaataaa taccatattt aaaacacagc cgttcaaggt cgtctgaata tcatttcaga
     9181 cgacctttca ccaatccccg ccgttttcca tctttctgct attgtgataa agtagcccga
     9241 ccttttgttc aaaatacgcg gattccgcga cactgaatgc agcatctgtc aaagattatt
     9301 tgaaaacggt cgagttagac aagtctgccg acaatgtcga taccacatcc aaaactatcc
     9361 gcagggttta ggcggtattc cagagtacgg tgtcttatgc tgatgctgta aacaatgccc
     9421 gtatagaaaa tctaaaacgg ttgttgccga ccgttatgcc ggtttatgag caaagtgtca
     9481 gaaataaggg gcgcgttaat atagtggatt aaatttaaat caggacaagg cgacgaagcc
     9541 gcagacagta cagatagtac ggcaaggcga ggcaacgctg tactggtttt tgttaatcca
     9601 ctataaaaaa cgtcggcgtt aagggaaggg gatcgagaat ttgagccgtt gtttcaaaat
     9661 gccgtctgaa atcgtttgag attcagacgg catttaaaca gttctgcgcc cgcccgtttt
     9721 gccttcaagc ccgcgccgct taaatccaat cccccctgca acgggcgcaa aaaaatctcc
     9781 gtgcctcgcg gtcggggatg tttcgggttt cacgcccgtc tgcgccgtgc cttgctgtcg
     9841 ggctatgccg tctgcaccgc cgccgtttcg gcttggtttg atgcggtcgg gcatttatgc
     9901 ggctacgccc tgatattcag ttcggcggtc ttgcctgttt tggcaggcat attcagcagc
     9961 gcgtctattg tgaacttgct gattttgccg tttgccaagt cggctacgca atggcttcga
    10021 tacccttgtt tttgacgatg gtcagcagct tcaacgccgc gccgctgggc tttttaacgc
    10081 cccgctccca agccgaaacg tgattttttc ccacgttgag atagatggcg aaagcggctt
    10141 gcgatagtgc ctccttctcc ctgattgcct tgatgtcccc gccgctcaac ggtttgattt
    10201 cggtcaggca ggacttgtca aagccgcgca tagtcttttt gtcgattgcg ccgatgtcgt
    10261 gcagcccctg catggtttca tgtattgctg cgaaaatctc acttttgtat ttcatggctg
    10321 tatctccgta aaagtgccgt ttgatttggc ttgctctatg tcggtttctg tcattgccaa
    10381 gatgatggcg gcggctttct ttaattctgc aagttctttg tccgtgatgt tttcgcggtc
    10441 gttcttggca aaggcggtca tgaaaaacgc ccgttctcca tacctgaaaa atatcaggct
    10501 gcgataaccg ccgcttctgc cttgtccttg ccttgctatg cgctgcttaa taacgccacc
    10561 gccaaggtct gcgtctatca gcccgttatc cgcccgctct accgcctcca gcagctcgga
    10621 atcactgatt ttatgtttct tggcaaattt cactatccat tggtttttga atatccgcat
    10681 tgtgtagccg ttgattagtg atacttggaa ttataccaga gacagacaac acggcaagct
    10741 gcctttttta tgccccgcaa tttctccgtt tatgccgcct gaaacactgg cggctaacct
    10801 gattattttt taatcaatcg cttttaatgg ctcaaattgc gtttttagcg cgtttcgctg
    10861 tcttcctatg tcccccatat ataaccaagc ggtttaaccg ccattcctgc tcaaaatagg
    10921 cgcggttccg tgcgttttgg cttttcattc cgttacgctc ccactgccaa gacggtgaaa
    10981 aaccggaaaa ccccaaaagt tgttaccgat tcgttaccat ttgttaccgc atttgttacc
    11041 aattttttat ctttttctt
//
LOCUS       DAWFBO010000061        10979 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFBO010000061 DAWFBO010000000
VERSION     DAWFBO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.061"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(14..604)
                     /locus_tag="WGO87_001103"
     CDS             complement(14..604)
                     /locus_tag="WGO87_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0392470.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            complement(665..1246)
                     /locus_tag="WGO87_001104"
     CDS             complement(665..1246)
                     /locus_tag="WGO87_001104"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0392471.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAELYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            1324..1557
                     /locus_tag="WGO87_001105"
     CDS             1324..1557
                     /locus_tag="WGO87_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392472.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            1730..2458
                     /gene="rpsB"
                     /locus_tag="WGO87_001106"
     CDS             1730..2458
                     /gene="rpsB"
                     /locus_tag="WGO87_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0392473.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            2587..3441
                     /locus_tag="WGO87_001107"
     CDS             2587..3441
                     /locus_tag="WGO87_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0392474.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPATVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            3658..4377
                     /locus_tag="WGO87_001108"
     CDS             3658..4377
                     /locus_tag="WGO87_001108"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0392475.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            4561..5523
                     /gene="mafA"
                     /locus_tag="WGO87_001109"
     CDS             4561..5523
                     /gene="mafA"
                     /locus_tag="WGO87_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0392476.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIQPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            5563..7008
                     /gene="mafB"
                     /locus_tag="WGO87_001110"
     CDS             5563..7008
                     /gene="mafB"
                     /locus_tag="WGO87_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0392477.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     SGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYHIKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDVRGIVQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            6974..7357
                     /locus_tag="WGO87_001111"
     CDS             6974..7357
                     /locus_tag="WGO87_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392478.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            7574..8290
                     /locus_tag="WGO87_001112"
                     /pseudo
     CDS             7574..8290
                     /locus_tag="WGO87_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            8295..8654
                     /locus_tag="WGO87_001113"
     CDS             8295..8654
                     /locus_tag="WGO87_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0392479.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            8730..8942
                     /locus_tag="WGO87_001114"
     CDS             8730..8942
                     /locus_tag="WGO87_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392480.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            9039..9884
                     /gene="mafB"
                     /locus_tag="WGO87_001115"
                     /pseudo
     CDS             9039..9884
                     /gene="mafB"
                     /locus_tag="WGO87_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            9894..10100
                     /locus_tag="WGO87_001116"
     CDS             9894..10100
                     /locus_tag="WGO87_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392481.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            10232..10483
                     /locus_tag="WGO87_001117"
     CDS             10232..10483
                     /locus_tag="WGO87_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392482.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            10480..10851
                     /locus_tag="WGO87_001118"
     CDS             10480..10851
                     /locus_tag="WGO87_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0392483.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT         3381 a         2516 c         2392 g         2690 t
ORIGIN      
        1 tgaaaatact gcctcacacc tgcacgccat accctgccaa cctgccggtc aggatttccc
       61 tgtttttgca ccaatcttcc ctcagcatac tgtacacgac cgtatcgcgc acactgccgt
      121 ctttacggag catatgcata cgcagcacgc cgtctttttc cgcacccagc cgttcgatgg
      181 cacgttgcga ggcaaggttc agaatatccg tgcgccatcc cacgcaacgg caagccaaaa
      241 catcaaatgc gtaatccaac agcatgattt tgcaacaggt gtttatccgt gtccgccgtg
      301 ccgatgccgc ataccatgtg aatccaatat ccaaacgcgg aatctgcggt tcaaaatgat
      361 aatacgccgt tgtcccgacc accctgcccg cctcttcatc gacaaccgca aacgccaaac
      421 gcgttgccaa tgctgtcccg atatagtctg ccaccctatc cggatggggc gcggacgtta
      481 ctcccagctt ccaaacttcc ccatcgcaaa ccgcctcgcg caaacccgtt tcatgatgca
      541 catccaacgg ttcgagccgt acgccaccca acgacaagac cggcagtatt atcttttccg
      601 acatcctttt ctcccaatat tccaccttca gacggcattt ccgttcaaaa tgccgtctga
      661 acggctaaaa acacaatatc cccgcctccg acacaaaacc gtccagcggc aggtcgtgcg
      721 cctcgaccga cagtctgtcc accaactggc aggcaaagcc cacgcccacg gtttttgcct
      781 gcaaacggta tttcatcgct gcaagcgtcg catcgtaata gccgcctgcc tgccccaaac
      841 ggtagccttc gcggtctatg ccgacaagcg gaacgagcaa taccgacaaa ccgtgcacgc
      901 ggattttgcg ccctgcaaac tgcggcacgt tcaacctcgc cctgccccgt atgcgctccc
      961 gttccattcc gctttcagga tacggcgtaa accacatccg ccgcgaacgc ggttcgatat
     1021 aaggcaggta gagttccgca ccgcgttttt gcgccgcgcg gacaaagccg tccaaacgca
     1081 attccttgcc catcggccaa tacacgccga ttttccgccc ttttttaata taacgtttga
     1141 gcaggtggtt gatttttacc gttgccgccg cccgcacgtc ccgccccatt tgcgaacgcc
     1201 gcccgcgcaa ttcgcggcgc agggcgcgtt tttcctcatt cctcatttca gacggccttt
     1261 caggattgcg gtagaatgtt gcgattataa cgattttgtt aacattcaaa caggacgcac
     1321 acaatgtggc acatcgtcgc catcggctat ctttttgttg ccgttatgta ttccgccgcg
     1381 cagccgagta ttgcgcgcgc cttgatttat ttggtttttt gggcggtgct gcccaccgtg
     1441 ttcacggttt tcaccattac cgtccgccgc cgcaaccacc tgatgaggca gcaggaacag
     1501 gcggaatccg aacagcagcg cgcacaacgg caaaaagaca gcggcacaaa accctgaatc
     1561 ccttttcaga cggcatctta tccgctataa tccgtcagtt ttccatttcg gaaacacact
     1621 attttttaaa acttatgccc actttcgccg aagggtgctt gacaataggc gtgacctatc
     1681 aagttctatg cgattgaatg tgtgctctta accctttcaa ggaaataaaa tgtctcaaat
     1741 tactatgcgt cagatgattg aagccggtgt tcacttcggc caccaaaccc gtttctggaa
     1801 cccgaaaatg gcacaataca ttttcggtgc gcgcaacaaa atccatatcg taaacttgga
     1861 aaaaaccctg ccgatgttcc aagacgcgca agaagccgta cgccgtttgg ttgccaacaa
     1921 aggtacagta ttgttcgtag gtaccaaacg ccaagcccgc gacatcatcc gcgaagaagc
     1981 gacccgtgcc ggtatgcctt tcgttgatta ccgctggttg ggcggtatgc tgaccaacta
     2041 caaaaccgtt aagcaatcca tcaaacgcct ggaagaaaaa accgcagcct tggaaaatgc
     2101 tgccgaaagc ggtttcagca aaaaagaaat tctggaaatg caacgcgatg ttgaaaaact
     2161 ggaacgttct ttgggcggta tcaaaaacat gaaaggcctg cctgacgcga ttttcgttat
     2221 cgataccggc taccaaaaag gtactctggt tgaagctgaa aaattgggca tccctgttat
     2281 cgccgtagtc gataccaaca acagccccga cggcgtgaaa tacgttatcc ccggcaacga
     2341 cgactccgcc aaagccatcc gcctgtactg ccgcggcatc gctgacgcag ttttggaagg
     2401 caaaaaccaa gcgctgcaag aaaccgtagc cgctgcccaa gaagccgctg ccgagtaatc
     2461 cggcaaaccg aagaggggcg ttatgcccct tttctcaaat atgccgtctg aacgtccgtt
     2521 cgcggcacac gattcccgaa tgcggaaaat cctttccgta tttcccaaaa atctaggaga
     2581 ttcaaaatgg cagaaattac tgcaaaaatg gttgccgatc tgcgcgccgc taccggcctg
     2641 ggcatgatgg aatgcaaaaa agccttggtt gaagccgaag gcaacttcga caaagccgaa
     2701 gaaatcctgc gtatcaaatc cggtgcgaaa gccggtaaac tggccggccg taccgctgcc
     2761 gaaggcgtat tggcttacgc gatcaacggc aatgtcggcg cattggtcga agtaaactgc
     2821 gaaaccgact tcgtcgcgaa agacgccggc ttcgtagaat ttgccaactt cgttgctaaa
     2881 actgctgccg agaaaaaacc ggctaccgtt gaagaactga gcgaactggt tgaagcagaa
     2941 cgcaaagcca tcatcgctaa attgggcgag aacatgtctg tccgtcgctt ccaagtgatc
     3001 gacactgcca accaactggt tgcctacatc cacggcgcat tggcgaccga aggcgtattg
     3061 gttgagtaca aaggctctga agacgtagca cgcaaaatcg gtatgcacat tgttgccgct
     3121 aaaccacaat gcgtaagcga agccgaagta gatgccgaaa ccgttgaaaa agaacgccac
     3181 atctacaccg aacaagccat cgcttccggc aaacctgccg acatcgccgc taaaatggtt
     3241 gaaggccgta tccgcaaatt cttggctgaa atcactctga acggccaagc attcgtgatg
     3301 aaccccgatc aaactgttgc ccaattcgcc aaagaaaacg gcactgaagt ggtcagcttc
     3361 gtccgctaca aagtaggcga tggcatcgaa aaagccgttg tcgactacgc agccgaagta
     3421 gccgcagccg ccaaagtgta aggcacttat gaaaaagaaa gcacctggat tccaaacgaa
     3481 tcagggtgct tttttttgag aaaaccgttt gcggtaccta ttttaagacg accgaatatt
     3541 cagaccgtct taaaacaaaa caataataaa ccgacacacc ctatcattaa tattccgacc
     3601 gttggaaatt cagacggccc aactccgacc gacgacattc aagaaagcaa ggtatccatg
     3661 acacagcaaa tcaaatacaa acgcgtatta ctgaaactct ccggcgaatc cctgatgggt
     3721 tccgatccgt tcggcatcaa tcacgatacc atcgttcaaa ctgtcggcga aattgccgaa
     3781 gtcgttaaaa tgggcgtgca agtcggtatt gttgtcggcg gcggcaatat tttccggggc
     3841 gtatccgccc aagcaggcag catggatcgc gccaccgccg actacatggg catgatggcg
     3901 accgtgatga acgcgttggc actcaaagac gcatttgaaa ctttaggcat caaagcgcgc
     3961 gtacaatccg cactgtctat gcagcaaatc gctgaaacct acgcccgccc caaagccatc
     4021 caatatttgg aagaaggcaa agtcgtgatt tttgccgccg gtaccggtaa cccgttcttc
     4081 acgaccgaca ctgccgccgc attgcgcggt gcggaaatga actgcgacgt gatgctcaaa
     4141 gccaccaacg ttgacggcgt gtacaccgca gaccccaaaa aagactcatc cgccacgcgc
     4201 tacgaaacca ttacttttga cgaagccttg ttgaaaaacc tcaaagtgat ggatgccacc
     4261 gctttcgccc tctgccgcga acgcaagctc aatattgtcg tattcggcat tgctaaagaa
     4321 ggctcgctca aacgtgtagt aaccggtgaa aacgaaggta cgctggttca ctgctgattg
     4381 accatagtgt cggcagatat agtcgcatat gggcttcaga cagccattta ttatatggag
     4441 attatagtgg attaaattta aaccagtacg gcgttgcctc gccttgccgt actggtttaa
     4501 atttaatcca ctatatttac aattttgata caatttgttt ttcatcaaag gagaaaatct
     4561 atgcaagcac ggctgctgat acctattctt ttttcagttt ttattttatc cgcctgcggg
     4621 acactgacag gtattccatc gcatggcgga ggtaaacgct ttgcggtcga acaagaactt
     4681 gtggccgctt ctgccagagc tgccgttaaa gacatggatt tacaggcatt acacggacga
     4741 aaagttgcat tgtacattgc aactatgggc gaccaaggtt caggcagttt gacagggggt
     4801 cgctactcca ttgatgcact gattcgtggc gaatacataa acagccctgc cgtccgtacc
     4861 gattacacct atccacgtta cgaaaccacc gctgaaacaa catcaggcgg tttgacaggt
     4921 ttaaccactt ctttatctac acttaatgcc cctgcactct ctcgcaccca atcagacggt
     4981 agcggaagta aaagcagtct gggcttaaat attggcggga tgggggatta tcgaaatgaa
     5041 accttgacga ctaacccgcg cgacactgcc tttctttccc acttggtaca gaccgtattt
     5101 ttcctgcgcg gcatagacgt tgtttctcct gccaatgccg atacggatgt gtttattaac
     5161 atcgacgtat tcggaacgat acgcaacaga accgaaatgc acctatacaa tgccgaaaca
     5221 ctgaaagccc aaacaaaact ggaatatttc gcagtagaca gaaccaataa aaaattgctc
     5281 atcaaaccaa aaaccaatgc gtttgaagct gcctataaag aaaattacgc attgtggatg
     5341 ggaccgtata aagtaagcaa aggaattaaa ccgacagaag gattaatggt cgatttctcc
     5401 gatatccaac catacggcaa tcatacgggt aactccgccc catccgtaga gactgataac
     5461 agtcatgagg ggtatggata cagcgatgaa gtagtgcgac aacatagaca agggcaacct
     5521 tgattcacac gaccataacc gcttgctacc aaggaaaaca aaatgaattt gcctattcaa
     5581 aaattcatga tgctgtttgc agcagcaata tcgttgctgc aaatccccat tagtcatgcg
     5641 aacggtttgg atgcccgttt gcgcgatgat atgcaggcaa aacactacga accgagtggc
     5701 aaataccatc tgttcggcga tggtcgcggc agtgttaaaa atcgggttta cgccgtccaa
     5761 acatttgatg caactgcggt cggccccata ctgcctatta cacacgaacg gacaggattt
     5821 gaaggtgtta tcggttatga aacccatttt tcagggcacg gacatgaagt acacagtccg
     5881 ttcgatcatc atgattcaaa aagcacttct gatttcagcg gcggtgtaga cggcggtttt
     5941 actgtttacc aacttcatcg aacagggtcg gaaattcatc cggaggatgg atatgacgga
     6001 ccgcaaggca gcgattatcc gccccccgga ggagcaaggg atatatacag ttaccatatc
     6061 aaaggaactt caacaaaaac aaagactaat attgtccctc aagccccatt ttcagaccgt
     6121 tggctaaaag aaaatgccgg tgccgcctct ggttttttca gccgtgcgga tgaagcagga
     6181 aaactgatat gggaaagcga ccccaataaa aattggtggg ctaaccgtat ggatgatgtt
     6241 cgcggcatcg tccaaggtgc agccaatcct tttctaacgg gttttcaggg attgggagtt
     6301 ggggcaatta cagacagtgc ggtaaacccg gtaacctatg cggcagcacg gaaaacttta
     6361 cagggtattc acaatttagg aaatttaagt ccggaagcac aacttgcggc cgcaaccgca
     6421 ttacaagaca gtgcttttgc ggtaaaagac agtatcaact ctgccagaca atgggctgat
     6481 gcccatccga acattacagc tactgcccaa actgcccttt ccgtagcaga ggctgcaggt
     6541 acggtttgga gaggtaaaaa agtagaactt aacccgacca aatgggattg ggttaaaaat
     6601 aacggctata aaaaacctgc tgttcgcctt atgcagactg tagacgggga gatggctggg
     6661 ggaaataagc caccgaaacc aagtacgcag caacactcta cacactctga taacaatata
     6721 ggcttacctg ccccatatgt taaacctgat acatctattt ctccgacagg aacaatttat
     6781 gtagacagca aaggaaatgc aataccaaca ccaataggcg gttatataac aggttcgcct
     6841 gatgctcgtt ttatccaagt ccgagatagt aatggaaact tgactggagc cagaatagat
     6901 gatgctcatc atgcaaatac ccatcaagac ccacgcgcac ttacaggaca cgcacatgta
     6961 ccaggagtta caaatgatga cggaacccca tggttaccca tcaaataatt tacctgttaa
     7021 aatcataaat gatattattt atgcaaccca gttagttgaa gatttggttt taggaaaaat
     7081 aaaaattgtt gattttttaa aatcatataa caatttttat tattggcttg gttttgatga
     7141 gttacctcaa tctgagaaaa taaaatttct aaactatctc aatatattaa gtattcataa
     7201 ggaaatacaa gataaaactg tgaatagggt ttataccgat tgttttgata ttgataaatt
     7261 acattcgctt ggtagaatta caactaatga atgtattaat ggtataaaaa aaatctatca
     7321 aaaaaacaaa ttagaatttg acaatatact caaataacta gcctatgttt tgtaaaataa
     7381 aaatattctt gctgagtgta gagccagaga taaaaagatt tgatagtagt ttgcagttgt
     7441 agaattatat aaacaagaaa atagtcagca gccgtaggtc agattctcga atccgacatt
     7501 ttccaacagc ggcatttcgg aaacgataga tgcgtcaaat atttttgtcg gatacaaata
     7561 tccgacctac atctctgcgc agcagactct acaaggtatt aatgatttag gaaatttaag
     7621 tccggaagca caacttgccg ccgtgagcct attacaggac agtgcttttg cggtaaaaga
     7681 cagtatcaac tctgccagac aatgggctga tgcccatccg aatataactg caacagccca
     7741 aactgccctt gccatagcag aggccgcagg tacggtttgg ggcggtaaaa aagtagaact
     7801 taacccgact aaatgggatt gggttaaaaa taccgattat aaaacacctg ctgcccgacc
     7861 tatgcagact ttagatgggg aaatggccgg tgggaataaa ccgcctaaat ctataacgtc
     7921 tggaggaaaa gccaatgctg caacttatcc tcaattagtt aatcaattaa ctgggcaaaa
     7981 cttaaaaaac attgcggctc aagatccaag attgagtcta gctcttcata agagtgaaaa
     8041 aaattttcca ataggaactg caacttatga agaggcagat aggctaggta aaatttgggt
     8101 tggtgagggt gcaagacaaa ctagtggagg cggatggtta agtatagatg gcactcgaca
     8161 atatcggcca ccaacagaaa aaaattcaca atttgcaact acaggtattc aagcaaattt
     8221 tgaaacttat actattgatt caaatggaaa aaggaataaa attaaaaatg gacatttaaa
     8281 tattaggtaa ttatatgaga ttaaaattaa aaaattatga ttgttctgac tttgatttgg
     8341 aagatttccc acaagacaag ttagaaaatt tttgtatact tttgacatta tcaataggtt
     8401 ttgatgaaag taatggtgct gattattttt atgtttatat atatagcact gaatggttac
     8461 tttcaaatat acatagaccg atgtcattaa aaaactcaat tgttacaaac cgttttaata
     8521 ttgaacacat attaaaattg ataaatgata tattggaaat atgtaattca acgtctgaag
     8581 ataagtctat atcaaatttg gcaaaatatt ttgactggga atttgacgat tataatctaa
     8641 atatccaaga ctaaagatgg tggacagcat ttagaccaaa aacaggcaaa cggtattttg
     8701 atgacgaatt aaaataaaag gaattattca tgaaattaat taattttgaa gagagttcag
     8761 ttaaaattag tctcgataaa gatgagttgt atattattca agcaattgta ggagaaattt
     8821 attcaggcgt atgcgtggat tgtagagatt ttgagataat tcatggtgtt gaaaaaataa
     8881 agttctttct ttggataaag aacttaaaaa aatatatgat acttgggata aatgttagct
     8941 aggtaaggtg ggcacttgtt gcccaccttt tgccgtatat taaaaatcct tttcaggctg
     9001 cctgaaactg ggtgggcatc aatgccaatc ctaactgtgc tgatgaagca ggaaaactga
     9061 tatgggaaaa cgaccccaat aaaaattggt gggctaaccg tatggatgat attcgcggca
     9121 tcgtccaagg tgcggttaat ccttttttaa tgggttttca aggagtaggg attggggcaa
     9181 ttacagacag tgcggtaagc cctgtcacag atacagccgc tcagcagact ctacaaggta
     9241 ttaatgattt aggaaaatta agtccggaag cacaacttgc tgccgcgagc ctattacagg
     9301 acagtgcctt tgcggtaaaa gacggcatta attccgccag acaatgggct gatgcccatc
     9361 cgaatataac agcaacagcc caaactgccc ttgccgtagc agaggccgca actacggttt
     9421 ggggcggtaa aaaagtagaa cttaacccga ccaaatggga ttgggttaaa aataccggct
     9481 ataaaacacc tgctgttcgc accatgcata ctttggatgg ggaaatggcc ggtgggaata
     9541 gaccgcctaa atctataacg tccaacagca aagcagatgc ttccacacaa ccgtctttac
     9601 aagcgcaact aattggagaa caaattagta gtgggcatgc ttataacaag catgtcataa
     9661 gacaacaaga atttacggat ttaaatatca attcaccagc agattttgct cggcatattg
     9721 aaaatattgt tagccatcca acaaatatga aagagttacc tcgcggtaga actgcgtatt
     9781 gggatgataa aacagggaca atagttatcc gagataaaaa ttctgacgat ggaggtacag
     9841 catttagacc aacatcaggt aaaaaatatt atgatgattt ataggaaaaa gccatgaata
     9901 tactatccat aaataatcaa aactcaacta tttcactaac tcaagatgaa gtttttgttt
     9961 tacgagctat cttgaatgag atatatgcgg gcgtatgtgt agattcaaga gaatttgaaa
    10021 atgtatctgg tgttagaaaa catgaagtag ataatttaca acaacagttt gctggaattt
    10081 ataaaaaaat gacaacttaa caacccaaat ttttgtcaga gcctagtgca aattacaact
    10141 atgattctat tgtagccgaa atgaaagaaa aaaatcatgg gttggcgaca gggttgatgt
    10201 tgttaatatg cctgatggag cacctactag tatggataac acgcgtatta tggcagcacg
    10261 tgaagcagga gtaaaagtgg aagcgaatgt tcataatttt aatgaccgat tatcatcaaa
    10321 agagagaatc aggtttaagc atgatggtat tgagcctcaa acttggggag aagctatcca
    10381 gctacgaatt agaaagcaag aaacacaaaa aggagttcca gaagggtgga gcaaaagatt
    10441 tcctaacgga agtatttatg atgtaaaggt acttaggaaa tgataaaaca aaatagtttt
    10501 gttccgtatc ctgaagcaat gcttcctaaa ggatttaaat atccgcaaag ttatttaaaa
    10561 ttagctcaat ccactcatgc cattaactac gatgaacaat attcttttcc ttggtggttt
    10621 gaaaatgcag aaagcaatat atcagaagta attgacattt attttgaaat aactggcatt
    10681 ccaaacctat taccttttgc tagaaaccaa gagtgggctg cctgttttga tatttcagat
    10741 aaatcaggta atcctaaaat tatagtagtt aatttagata atacaaaata ttacgagact
    10801 tttgaaaatt ttgatacttg gctaaaagaa gctgaaaatg atggttggta gcagccgtag
    10861 gtcggattct cgaatccgac attttccaac agcggcattt cggaaacgat agatgcgtca
    10921 aatatttttg tcggatacaa atatccgacc tacatctctg cgcagcaaac tttacaaga
//
LOCUS       DAWFBO010000062        10684 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFBO010000062 DAWFBO010000000
VERSION     DAWFBO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10684
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.062"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(14..1216)
                     /gene="trpB"
                     /locus_tag="WGO87_001119"
     CDS             complement(14..1216)
                     /gene="trpB"
                     /locus_tag="WGO87_001119"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0392484.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTAAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPQMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1299..1988)
                     /locus_tag="WGO87_001120"
     CDS             complement(1299..1988)
                     /locus_tag="WGO87_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0392485.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLRERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            2185..2964
                     /gene="rsmA"
                     /locus_tag="WGO87_001121"
     CDS             2185..2964
                     /gene="rsmA"
                     /locus_tag="WGO87_001121"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0392486.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVIEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNNIEGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGRADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKVV"
     gene            3137..3865
                     /locus_tag="WGO87_001122"
     CDS             3137..3865
                     /locus_tag="WGO87_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0392487.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENIALAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            3938..4297
                     /locus_tag="WGO87_001123"
     CDS             3938..4297
                     /locus_tag="WGO87_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392488.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMSLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            4302..4748
                     /locus_tag="WGO87_001124"
     CDS             4302..4748
                     /locus_tag="WGO87_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0392489.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRKLKVWVLWRQPKKTAETDTSD"
     gene            4779..6053
                     /gene="folC"
                     /locus_tag="WGO87_001125"
     CDS             4779..6053
                     /gene="folC"
                     /locus_tag="WGO87_001125"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0392490.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPKSLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACALTVLECLNAKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALHRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6066..7091
                     /gene="ftsN"
                     /locus_tag="WGO87_001126"
     CDS             6066..7091
                     /gene="ftsN"
                     /locus_tag="WGO87_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0392491.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLESSAKPKQEEAAEKPQQAETAKTAPKQAKQRAAEKVPATADS
                     TDTVAVEKPKRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSD
                     SAVKEAKKADKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGY
                     AEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGI
                     AGQVTNE"
     gene            7084..7581
                     /locus_tag="WGO87_001127"
     CDS             7084..7581
                     /locus_tag="WGO87_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0392492.1"
                     /translation="MNNLPVADLLVSAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAASFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLKHADNRDG
                     ALEDD"
     gene            7790..9334
                     /gene="purF"
                     /locus_tag="WGO87_001128"
     CDS             7790..9334
                     /gene="purF"
                     /locus_tag="WGO87_001128"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0392493.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTIITRQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRVEHSISIS
                     DAGDEE"
     gene            9434..9925
                     /gene="greB"
                     /locus_tag="WGO87_001129"
     CDS             9434..9925
                     /gene="greB"
                     /locus_tag="WGO87_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0392494.1"
                     /translation="MSTETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            9984..10610
                     /locus_tag="WGO87_001130"
     CDS             9984..10610
                     /locus_tag="WGO87_001130"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0392495.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         2665 a         3099 c         2764 g         2156 t
ORIGIN      
        1 cagacggacg aggttacagt ttaatccctt tgagcttcgc cacggtattg atgtctttgt
       61 cgccacgacc tgagaggttg accaaaatca cttggtcttt gcccatttgc ggcgcgtttt
      121 tcaccgccca agcaacggca tggctggatt cgagagcagg aataatgccc tcgaagcggc
      181 agagcaagtc aaaggcttcg agtgcttcgt cgtctttggc ggcggtgtat tcgacgcgct
      241 tgatgtcgtg cagatggctg tgttccgggc cgatgccggg gtaatccaag cctgcggaaa
      301 cagagtgcgt acccaaaacc tggccgtttt cgtcctgcat cagatagctg cggaaaccgt
      361 gcaatacgcc aatcggtgcg cccgaagtaa tcggcgcggc gtgatcgggg gtgttcacgc
      421 ccaaaccgcc ggcctccacg ccgacgaggc gcacgttttc ttcgccgata tacgggtgga
      481 acaaaccgat ggcgttcgat ccgccgccca cgcaggcaac ggcaacgtcg ggctgtctgc
      541 cgatggcttc caacatttgt tctttggctt cattgccgat cacgcattgg aaatcgcgca
      601 ccatttcggg atacggcgcg gggccagcgg cggtaccgat gatgtagaac gtgtcgtcca
      661 cgcgggcgac ccattcgcgc atggcttcgt tcatcgcgtc tttcagcgtg cggctgccgc
      721 tttctacacc gaccacgttc gcacccaata atttcatacg gaacacgttg ggcatttggc
      781 gttggatgtc gtccgcgccc atatacacgt cgcaagtcat accgaagcgt gcggcaacgg
      841 tggcactcgc cacgccgtgc tgacccgcgc cggtttcggc gatgacgcgt tttttaccca
      901 tgcgttttgc caacagtgcc tgaccgatgg tgttgttgac tttgtgcgcg ccggtgtggt
      961 tcaagtcttc gcgcttcaac cagatttgcg cgccacccag atgttcggac aaccgcgcgg
     1021 cgtggtaaac ggggctgggc ctgccgacgt agtgtttcaa atcgcggtgg aattcttccc
     1081 aaaacgacgg gtcgtttttc gctgccttat aggcatccgc cagctcttgc aaagcgggaa
     1141 tcagggtctc ggacacgaac agcccgccgt gttcgccgaa aaagcccttc tcgtcgggcg
     1201 cgtggtagtt cttcatcgga ttcttccttg tgcttcaggt tttcagacgg catttccgtt
     1261 ttttcaaaca tgccgtctga acgggaaatt gtcggcaatt ataactgttt ttcacacaag
     1321 cccgccattt caaaaaacgc ccgatacgcc gccgcttttt tcgccaaact caaaggatag
     1381 gcgcgcgaag tcgaaggcag gcgcgtcagc gtcaaatccc gtccggcaaa cggaaacggc
     1441 acggtttcgc ccgttttcgg cattttgata ccgccaccct gaatatcgag caggatttcc
     1501 gtcgccttgc cgcctgtcgt acaaatatgg cggcactcgg gtatttttgc caacaccgcc
     1561 gccaaatcga cggtttcaac tacctttaaa aacttgtcgg acgcattgcc gtgctgacgt
     1621 accgccttca acacggtcgg acaggacgca attccccgtt cgcgcaaaaa cgccttgatt
     1681 ttctcggcat caaacgcttt ttcagacaac ctttggaaat gcgccgcatc attaaaaaac
     1741 accagcccgt aaacgcgcca catatcgttt tggaaattcg gataatgaaa ctgcatcgcg
     1801 cgtttgtctt ccttgggcgg aaacgtcccc atcatcatga ccgcagcctt cggcggcaac
     1861 acggaatcga agggatgggt ttcgatttca agcagatttt cagacacgtt caatcctcca
     1921 acaggcaaaa cggcagacag ccggtttttg caccaaaccg ccaaacacgg caagccttca
     1981 aacagcatta tcacgccctg ccgttttcgg ttacaatgcc gacatcaaac ggatactgta
     2041 aacacattta tgtttcaaca cacaggacga cacttaaagc accgccctat gtgccatcct
     2101 gatttggaag gggttacgcc cctcccaaat agagtctgat tctaccgccc taaagggcgg
     2161 ggtttcaacc gaaaaggaaa cacgatgaaa gaacacaaag cccgcaagcg tttcgggcag
     2221 aattttttgc aggacacgcg gattatcagc gatattgtca acgccgtgcg cccgcaggcg
     2281 gatgatgtcg tgattgaaat cggcccgggt ttggcggcga ttaccgaacc tttggcgaaa
     2341 aaactgaacc gcctgcacgt tatcgaaatc gaccgcgaca tcgtatgccg tctgaaaacg
     2401 ctgccgtttg cggataaact cgtcattcat gaaggcgacg tgttgcagtt tgatttcaac
     2461 aacattgaag gcaaaaagaa aatcgtcggc aacctgccgt acaacatttc cacgccgctt
     2521 ttgttcaagc tggcggaggt ggcggacgat gtcgtcgata tgcactttat gctgcaaaaa
     2581 gaagtggtcg agcgcatggt tgccgcgccg aaaagcaacg actacgggcg tttgggcgtg
     2641 atgctgcaat atttcttcga tatggaaatg ctgattgacg tgccgcccga atcgttcgac
     2701 cctgcgccga aagtggattc cgccgtcgtc cgtatgattc cggtgaaaca ccgcatcggc
     2761 agggcggacg atttcgagca ttttgccaaa ctcgtgaaac tcgccttcca ccaacgccgc
     2821 aaaaccatac gcaacaatct gaaagagctt gcaggcgacg atgatttgca ggcagtcggc
     2881 atcaatccgc aagaccgcgc cgaacatatc gcgccagaga aatacgtggc gttgagcaat
     2941 tatctggcgg gcaaggtcgt ctgaaaaggg agtaagccga tgacgaaata cctgctttta
     3001 cccctgatac tgccgcttgc agcctgcctg accaatccga acgaccgttt ttttgacggc
     3061 agacgctaca cgcttcatca tatggagctc ccgtaacgac aacctgcaac ggcaaatgcc
     3121 gtctgaaaga aaagacatga tcaaattcaa aaacgtacac aaacatttca aagacctgca
     3181 cgtcatcaac ggcgtgaact tggaaatcaa aaaaggcgaa gtggtcgtcg tctgcgggcc
     3241 ttcgggcagc ggcaaatcca cactcatccg caccgtcaac cagcttgaaa gcatcgaaag
     3301 cggcgagatt tgggtggacg gtgtcaacgt tgccgaccca aaaaccgatt tgaacaaaat
     3361 ccgcgaagaa gtcggctttg tgtttcaaag tttcaacctc tacccgcacc tgaccgtgtt
     3421 ggaaaacatc gccctcgcac cgatgaaggt taaggggcaa aatgcggaac aggcggagaa
     3481 aaaggcaatg gagcttttgg aacgcgtcgg actggcacac aaaaaagacg ccttcccttc
     3541 ccaactttcc ggcggccagc agcagcgcgt ggcgattgca cgcggtttgg cgatggaacc
     3601 gcgcgtaatg ttgtttgacg aaccgacctc cgcactcgac cccgaaatgg tcggcgaagt
     3661 gttgaaagtg atgaaagact tggcagaaag cggcatgacc atgatgtgcg taacccacga
     3721 aatggggttt gcccgcgaag ttgccgaccg cgtgattttc gtcgataaag ggcaaatcct
     3781 cgaagacgaa acgcccgaag cgttttttac caacccgaaa cacgaacgcg ccaagcagtt
     3841 cctgcaacag gtcatgaccc actgatgcta taccgtctga aaaccgaacg ttcagacggc
     3901 atagcccgac accgcaagat gattgagaaa gaaaaccatg tattttgttg accgcaccgc
     3961 cgtcgtcctc aagccgaccg tccgcttttt ggaatggctc aaaagcaccg acgaaaatat
     4021 gcccgacctg accatagagc agcttcgtgc caactgctcc gtcttcctcg ttccgcagtt
     4081 cgacgagccg gaagccgtcg tttcctattt cgacgaacgc taccgccaga ttttcgaggc
     4141 ggagctggca ggttgggaca tcgataagga taaatggccg caagatatga gcttgaaagc
     4201 gttttgggag tttttcgaca tcgaaatcca cgatatggtt ttagacatgg aagaagcgga
     4261 attgaacatt acccccgtgt tcgacaacat gatgtaaggc tgtgcgcgcc tttcagacgg
     4321 cattgaggcc gtcccgcatc ctgaacatcc tgaccgtatc gctccactcg gcttcccttg
     4381 ccgtctgcct gacctggttt tacggccgga tgatgtggtt cggtttggcg gctttggcgg
     4441 caagttacgc atactcgctc aggataacga atctgaaaca ccgccatgcc ataaccgcca
     4501 ttaccataga ccgcgacgga caggcggaaa tcgtatccgg caaagaaaaa acggcaaagg
     4561 cggcggcact gtcgggcagc agtatggtta cgccttatgc cttattctta caatgggaca
     4621 ccggcggcaa aaccgtccgc cactgcatca cccacgatat ggcggacccg gaatcctacc
     4681 gcaagctgaa agtctgggta ctttggcggc agccgaaaaa aaccgccgaa accgatacat
     4741 cagactgatt tcagacggca tttgaaaacg aaacactcat gaaaacacta caagactggc
     4801 tctcccattt ggaaaccgcc cacagcggcg gcttgatcga tatgggcttg gaacgcgtaa
     4861 gcgaagtgaa aaaacggatg aagctcacgc cgcaatgccc cgtcgtcgtt gtcgcgggaa
     4921 caaacggcaa aggttcggtc tgcgcctacc tgacacagat ttacaaacag gcgggctaca
     4981 aaaccggcac actgaccagc ccgcatttat tgcgttacaa cgaacgcatc gccatcaacg
     5041 ccgaacccgt ttcggacgat acgattattg cctctttcga gcgcatcgaa gccgcgcgcg
     5101 gcgaaatatc actgacttat tttgaattca atacgttggc ggcggtcgac atcttcatgc
     5161 gcgaacaggt tgacgtgatg atattggaag tcggcttggg cggacgcttg gacgcggtca
     5221 acgcgtttga cggcgattgc gcggtggtta ccagcgtgga tttggaccat caggcatttt
     5281 tgggcgatac ggtcgagcag gtcggctttg aaaaagcagg cgtgttccgc agcggcaaac
     5341 ccgccatctg cggtcaaaac cccgcgccca aatcgttgct ttcacacgcc gaagccatag
     5401 gcgcgaaact gctgatggtg cagcgcgatt tcgagtttca cgcgatggaa aacatccaat
     5461 ggaactaccg cttccggccg caacattcag acgaccccgc acgcaaccgc aatgccctgc
     5521 ctttccctgc attacgcggc acataccagc tttccaacgc cgcctgcgcg ttgaccgtat
     5581 tggaatgctt gaacgccaaa ttgcctgtgg acatcggcgc catcaaacgc ggattgctgc
     5641 tggttgaaaa tcccggacgc ttccaagtcc tgcccggccg tccgctgacc gttttggatg
     5701 tcggacacaa tccccacgcc gcccgcgccc tgcaccgcag cctgattaat ttggcgtttg
     5761 cgcaaaaacg caccgccgta ttcagtatgc tgtccgacaa agacatagac ggcgtgttgg
     5821 aaatcgttaa agaccagttt gacgaatggt atatcgcgcc tttggacgtg ccgcgcggca
     5881 tgacgctcga gagcctgcaa aacaaactgc acgaacacag catcgaaaac atacagacat
     5941 tcgccgccgt ccgcgaagca taccgggcgg cagcctcaaa agcgggcgag aacgacagaa
     6001 tcgtcgtttt cggctcattt cataccgttg ccgatgtcat gtcggtactg taaaaggaaa
     6061 cttctatgtc cgaaaacaaa caaaacgaag tcctgagcgg ttacgaacaa ctcaaacggc
     6121 gcaaccgccg ccgcctcgta acggcaagtt gcctggttgc cgcctcctgc atcctgctgg
     6181 cagccgccct cagttccggc cctgccgaac agactgccgg cgaaacaagc ggcgtagaaa
     6241 acaaagcggc aggtgcggca caaacccctg ccttgaaatc cgccgccgac aaaccgcagg
     6301 acttggcagg cgaagacaag ccttctgccg ccgacagcga aatcagcgag cctgaaaacg
     6361 taggcgcgcc gctggtgctg attaacgacc gcctcgaaga cagcaatatc aaaggcttgg
     6421 agtcatccgc aaaaccgaaa caggaagaag ctgccgaaaa accgcagcag gcagaaactg
     6481 ccaaaaccgc accgaagcag gcaaaacaac gcgctgccga aaaagtgccg gcaactgccg
     6541 acagtacgga tacggtagcg gttgaaaaac cgaaacgcac tgccgaaaca aaaccgcaaa
     6601 aagcggaacg cactgccaaa gccaagccca aagccaaaga aaccaaaacc gccgaaaaag
     6661 ttgccgacaa accgaaaact gccgccgaaa aaaccaaacc ggatacggca aaatccgaca
     6721 gcgcggtaaa agaagcgaaa aaagccgaca aggctgaaag caaaaaaaca gccgaaaaag
     6781 accgttcgga cggcaaaaaa cacgaaacgg cacaaaaaac cgacaaagcg gacaagacca
     6841 aaaccgccga gaaggaaaaa tccggtaaaa aagccgccat tcaggcaggt tatgccgaaa
     6901 aagaacgcgc cttaagcctc cagcgcaaaa tgaaggcggc gggtatcgat tcgaccatca
     6961 ccgaaattat gaccgacaac ggcaaagttt accgcgtcaa atcaagcaac tataaaaacg
     7021 caagggatgc cgaacgcgat ttgaacaaat tgcgcgtaca cggtatcgcc ggtcaggtaa
     7081 cgaatgaata acctccccgt tgccgacctc ctcgtctccg ccatcatcgc cgcctgcatc
     7141 gtcatttcca cgatgcgcgg cgtgattgcg gaggcaggtt cgatggcggc atgggtggtt
     7201 tccttctttt tcgccaaact ctttgccgcc tccttcgccg acctcgcctt tgcctcgttc
     7261 caaccccgcc tgtttgcatt ggctctgtcg ttcatttccc tgttcgtcat tgcctgtctg
     7321 atccagaaaa tgctccgttc gctgctgacc ggcgcagttt cggcggtcgg tttgggcttt
     7381 gccaaccgca ttttgggcgg cgtattcggt gcattgaaag gcgttttgat tgttaccctg
     7441 ctgatcatgc ttgcttcaaa aaccgacctg cccgataccg aagaatggcg gcaatcttac
     7501 acactgccgt ttttcgtatc gctttccgaa gcagtgttaa agcatgcgga caacagggat
     7561 ggagctttgg aagacgattg attgccatac ggatatttca gacggcattc tcttttatgc
     7621 cgtctgaaat gtttttttcc tttcctcagc catatcacag attaaatctt atcctccata
     7681 aacccaccta tctgtccttc gctcaaatat tgtcaacaat tcaggcaaaa aaccaccgtt
     7741 tgctttataa ttccgttcta cccttaaacc tcgaatcgga gaagacgata tgtgcggcgt
     7801 attaggtttg gtcagtcatg aacccgtgaa ccagcttctg tacgacggtt tgcagatgtt
     7861 gcagcacagg gggcaggatg cggcgggcat tgcaacggcg gaaggcggca ccttccatat
     7921 gcacaaaggc aaagggatgg tgcgcgaagt gttccgcacg cgcaatatgc gcgatttggt
     7981 cggcaacgcc ggcatcgccc acgtccgtta tcccacggcg ggcaacgccg gcagcagcgc
     8041 ggaggcgcag cctttctacg tcagctcgcc gttcggcatc gttttggcgc acaacggcaa
     8101 cctcaccaac actgccgaac tgtatgaaaa cgtgtgcaac aaacacctgc gccacgtcaa
     8161 caccagctcc gattccgaag tcttgctcaa cgtattcgcg cacgaattac gccgcgaagt
     8221 ctctaaaaac gccgacccgc accggctcaa tgccgacaat attttcaacg ccgttgccga
     8281 agtccaccgc ctggtgcgcg gcgcatacgg cgtggttgcc atgattgcgg gctacggtat
     8341 gcttgccttc cgcgaccctt acggcatccg cccgctggta ttgggttcgc aaaccgacaa
     8401 cgaaggcagg aaatcctatg cagtcgcctc cgaatccgtc gccttcaacg cgcttaccta
     8461 cgatttggaa cgcgatatcc ggccgggcga agcggtattt gtcggctttg acggcacaat
     8521 catcacccga caatgcagcg accgcgccaa actcagcccc tgcctttttg aatatgttta
     8581 ttttgcccgc cccgactccg taatcgacgg cgtatccgtt taccagtcgc gcttggatat
     8641 gggcgtgtcc ttggcggaaa aaatcaaacg cgagctgccc gtggacgaca tcgatgtcgt
     8701 gatgcccatt cccgacacca gccgccccag cgcgatggag cttgccgtcc acctcgacaa
     8761 accctaccgc gagggcttga ttaaaaaccg ctatatcggc cgcaccttta ttatgcccgg
     8821 ccaggcgacg cgcaaaaaat ccgtgcgcca aaaactcagc ccgatggaaa ccgagtttgc
     8881 aggcaaaagc gtgttgctgg tggacgactc catcgtgcgc gggacgacca gccgcgaaat
     8941 cgtcgaaatg gtacgcgcgg cgggcgcgcg caaagtctat atcgcctccg ccgcccccga
     9001 agtgcgctat cccaacgtat acggcatcga tatgcccacg cgcgaagagt tgattgccaa
     9061 cgggcgcagc gcggcggaaa tcgccgccga aatcggcgcg gacggcatcg tgttccaaaa
     9121 tttgagcgat ttggaagccg tcgtcaaagc actcaatccg aaaatcgaat ccttcgattc
     9181 gtcctgtttc aacggcattt atcagaccgg cgacatcgat gatgcctacc tcgaccgcct
     9241 gtctgccgag aaatccggct gcggcggcct gaaaatccac ccgagcaggg tggaacacag
     9301 catcagcatc agcgatgcgg gcgacgaaga ataaggcttc ccaaaccgcg atgccgtctg
     9361 aaatccgaac gccgggtttt cagacgacat ttcccatatc ggcatcacat ttcccatttc
     9421 atccgcacgc aatatgagta ccgaaaccaa aaactacatt acacccgccg gctggcaggc
     9481 attgaaagac gaactttacc ggctggtcaa caaagaacgc cccgaaatcg tccaaatcgt
     9541 caattgggcg gcaggcaacg gcgaccgcag cgaaaacggc gactatcttt acggcaaacg
     9601 ccgtatgcgc gaaatcgacc gccgcatccg tttcctgacc aagcgtttgg aggctgccgt
     9661 cgtcgtcgat cccgaattgc gcgaagcgac cgaccaagta tttttcggcg cgacggtcgg
     9721 gctgttgcgc ggcgacgggc gcgagcaaac cgtcaaaatt gtcggcatcg atgaaatcga
     9781 taccgcgcaa aacaaaattt cttggatttc gccgttggcg cgttgcctga ttaaagcgcg
     9841 cgaaggcgac gaagtggttt tgaacacgcc ggaagggcgt gaggaaattg aaatcctttc
     9901 tgtcgaatac atcaaaatcg actgatcggg aatgccgtct gaaacggcac aaacgcatcg
     9961 tttcccaaat aaaggaatac cgaatgagga aaatccgcac caaaatctgc ggcatcacca
    10021 cactggaaga cgcagcggcc gccgcagcgg caggtgcgga tgccgtcggg ctggtctttt
    10081 tccaaggcag cagccgggcc gtcgatattg cccgcgcaaa aaaaatcacc gccgcactgc
    10141 cgccgtttgt cagcgttgtc gcccttttcg tcaacgaaag cgcgcaaaac atccgccgca
    10201 tccttgccga agtgccgata cacatcatcc aattccacgg cgacgaagac gacgcattct
    10261 gccgccagtt cgaccgcccc tatatcaagg ccattcgtgt tcagacgaca tcagacatcc
    10321 gaaacgccgc cgaccgcttc cccgacgctc aggcactgct tttcgatgcc taccatcctt
    10381 cggaatacgg cggcaccgga caccgcttcg actggacgct gctggcggaa tattcgggca
    10441 aaccgtgggt acttgccggc gggctgaccc ctgaaaacgt cggcgaagcc gtccgcatca
    10501 ccggagcgga agcggtcgat gtatccggcg gcgtggaagc gtctaaaggc aaaaaagacc
    10561 cagccaaagt cgccgccttt atcgcaaccg ccaaccgcct atcccgttaa agcaacaaaa
    10621 attgccgccg gaatgactta tagtggatta acaaaaacca gtacggcgtt gtctcgcctt
    10681 agct
//
LOCUS       DAWFBO010000063        10638 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFBO010000063 DAWFBO010000000
VERSION     DAWFBO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.063"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(120..1337)
                     /locus_tag="WGO87_001131"
     CDS             complement(120..1337)
                     /locus_tag="WGO87_001131"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0392496.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEAHRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1539..2267)
                     /gene="rph"
                     /locus_tag="WGO87_001132"
     CDS             complement(1539..2267)
                     /gene="rph"
                     /locus_tag="WGO87_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0392497.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2349..2813)
                     /locus_tag="WGO87_001133"
     CDS             complement(2349..2813)
                     /locus_tag="WGO87_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0392498.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNSARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3030..3806
                     /locus_tag="WGO87_001134"
     CDS             3030..3806
                     /locus_tag="WGO87_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0392499.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMATYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKAKMRVPCVFSTENITPPQSGVAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            3944..4471
                     /locus_tag="WGO87_001135"
     CDS             3944..4471
                     /locus_tag="WGO87_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392500.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKIYILRISFT
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRAS
                     TDYIVSRNLKFRHLY"
     gene            4642..5496
                     /locus_tag="WGO87_001136"
     CDS             4642..5496
                     /locus_tag="WGO87_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0392501.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFISLLELAKEGLVRIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5537..6745
                     /gene="pncB"
                     /locus_tag="WGO87_001137"
     CDS             5537..6745
                     /gene="pncB"
                     /locus_tag="WGO87_001137"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0392502.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6837..8555
                     /gene="argS"
                     /locus_tag="WGO87_001138"
     CDS             6837..8555
                     /gene="argS"
                     /locus_tag="WGO87_001138"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0392503.1"
                     /translation="MNLHQTVEREAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALADNAVIESAEVAGPGFINLRLRPDFLAQNIQTALNDT
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEAPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDADAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9121..10539
                     /locus_tag="WGO87_001139"
     CDS             9121..10539
                     /locus_tag="WGO87_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0392504.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPTPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2523 a         3002 c         2632 g         2481 t
ORIGIN      
        1 tttaatccac tatatttttt gttccaaagt caaaatatgc cgtccgaaca ttcgggcggc
       61 agacaaaacg gcactgcccg ataaaggcag tgccgttgtc cgtttcaaac cgtgaaacat
      121 cagcccaaat taaaggcttt atgcaatacc ctggttgcca gttccatgta tttttcatca
      181 atcaatacgg aaactttgat ttcggaggtg gaaatcattt ggatgttgat accctcttcg
      241 gcgagcgtgc ggaagatttt ggcggctaca ccgacgtgcg aacgcatacc caaaccgact
      301 gcggagactt tgcatacggt gtcgtcgcca tcaatagaag ccgcgccgat actgtcttgg
      361 cgttccgaca ggatttccaa agtctgcttg taatcgccgc gcggtacggt aaaggaaaaa
      421 tcggttgtgc cttcgctgcc gacattttgg ataatcatat cgacttcaat gttggcatcg
      481 gcaaccgcgc ctaaaatctg ataggcaacg ccgggtttgt cgggtacgcc gcgcacgttg
      541 atgcgggctt ggtttttatc gaatgcgata ccggttacgg cagctctttc catgttgtcg
      601 tcctcttcaa aggtaattaa ggtgccattg ccgccgtctt gcaggctgct cagtacgcgc
      661 aggcgcactt tgtattttcc ggcgaattct actgaacgga tttgcaaaac tttcgaaccg
      721 aggcttgcca gttcgatcat ttcttcaaat gtaaccgtat ccatgcggtg cgcttcgggt
      781 acgacgcggg ggtcggttgt gtaaacgccg tccacgtcgg tatagatttg gcattcgtcc
      841 gctttgaggg ctgcggcaag cgcaacggcg gaagtgtcgg aaccgccgcg tccgagcgtg
      901 gaaatatcgc cttcactgct gatgccttgg aagccggcaa cgatgacgac tttgccggcg
      961 gtaaggtcgg cacgcatttt ttcgtcatca atgctttcga tgcgggcttt ggtgtgggcg
     1021 gtatcggttt tgagggcgac ctgccagccg gtgtagcttt tggcttccac gccgatgtct
     1081 ttcaatgcca tcgccaaaag accgatggtg acttgttcgc cggtggacaa aaccacgtcc
     1141 aattcgcgcg gatcgggatg ctcctgcatt tcgtgcgcca acgcgaccag gcggttggtt
     1201 tcaccgctca tggcggatac gacgacgacg atgtcgtgtc cttcggcgcg ggctttggcg
     1261 acacgtttgg ccacgttttt aatgcgttcg ggcgagccta cggatgtgcc gccgtatttg
     1321 tgtacgatta acgccatgtt tcgtgctttc ttgtgtggga ttggtcgggc agcatggttt
     1381 gctggaaaag cggctattat tactattttt tacatgaagt tcaagaacgg actgcgcttt
     1441 cccgcctgcc gtttgacagc ggtcagcgaa aaacctgttc tttcagattg ttgacaaaat
     1501 gccgtctgaa cggttttcag acggcatccg gacgacaatc aggcggcgga caacgcattt
     1561 tgctggtgtt gcagcagttc gcctatgcct ttttgcgcca gtgcaaccag tttgcccaat
     1621 tcgtccaaac tgaacggcgc gtcttccgcc gtaccttgga tttcgatgat ttttcccgat
     1681 gcggtcatga cgatattcac atcactgtcg caaccggagt cttcgggata atccaaatcc
     1741 aaaagcggca cgccgttcac tacgcctact gacacagcgg caacggcttc gcggatgggg
     1801 ttttcactca aaatgccgtc tgaaaccagt ttgccgacgg cgatttgcag cgcgacaaac
     1861 gcaccggtaa tcgaagccgt gcgcgtaccg ccatctgcct gaatcacatc gcagtcaatc
     1921 aagatttgtc gttcgccgag tttttccata tccacgaccg cgcgtagcga acgcccgata
     1981 aggcgttgga tttcttgtgt gcgcccggac tgtttgcccg ccgaagcttc gcggcgcatc
     2041 cgggaagcag ttgaggcagg cagcatcccg tattccgccg ttacccagcc ttggttttta
     2101 ccgcgcagaa acggcgggac gttttcatct atggaagcgg tacaaatcac tttggtattg
     2161 ccgcattcaa taaggcacga accgtccgta tgcggcagga aatgaggggt gattttgata
     2221 tcgcgcaggc tgtcggcggc gcgcgagatg cggatgtaat caggcatact gccctcccgt
     2281 taaaaacaga taaattaaaa agccttaaat atgaaaaatc acatttaagg ccttcaaact
     2341 gaaaatttct acgcctcttc ggctttgctg cggataatca aaagcggcag gtggctttgg
     2401 cgcattaccg tttcggcaaa actgcccatt aaaaggtgca tcagcccggt acgtccgtgc
     2461 gtacccaaca ccagcaggtc ggcaccgttt tcatcggcat aatcaaccaa atcctgcgcc
     2521 atttcacgcg cacccttatt ggcaaccagc aggtgtttga cggtattttc cacacccagt
     2581 tcccgggcgg tgcgctcggc ggcatccaaa acttcgttgc cttgcgcgac ggcggcggct
     2641 tcgtagcttt cgtgttgcaa aaattcgggg gcgagtgcca tatattcggc aggattggca
     2701 acgtgcacca aagtcaggcg cgcgctgttg actcctgcaa gttcggcggc atgtttcagg
     2761 gcattgatgg acgtttcact gccgtcaacg gcaacgacca aatgtttgta catatcgtat
     2821 tctccttttg caccgcctcg cggtgccctc ttgtcggatg ggcgcaggga cagtttgcac
     2881 tgtttcatta tagacccgcc gtcgggcttt atacaacagc cgaacagccc gaccgctttc
     2941 cagtataata tgccgcttcc gtgcagtcag gcattttttg ccggctttcg ttcacttttt
     3001 gatttgacgc aatcttgcag gattcgacca tgtccgacaa cgctttgacc tcttcgcgac
     3061 gcttcggcgg catcgccaga ctctacggag actctgcctt ggcgcacttt tcacaggcac
     3121 acgtctgcgt agtcggcgtg ggcggtgtcg gctcgtgggc ggtcgaggct ttggcgcgga
     3181 cgggcatcgg acgtttgact ttgattgatt tggacaacgt tgccgaatcc aatgtcaacc
     3241 gccagctgca cgccctaact ggcgacttcg gcaaagcaaa agttaccgcc ttgcgcgaac
     3301 gcattacaca aatcaatccg caatgcgaag tgtttgaaat tgaagatttc gttaccgaag
     3361 acaatttgcc ggaatacttc ggaaaaggtt ttgatttcgt catcgacgcg atcgaccaag
     3421 tgcgcgtcaa agcggcaatg gcgacttatt ttgtggaacg caaacaaccg tttgtcctca
     3481 gcggcggcgc gggcggacaa aaaaatccgg cgttaatcca aaccgccgat ttgagccgcg
     3541 taacccacga cccgctgctt gccaacctgc gctacacctt gcggaaacgc tacggcttta
     3601 gccgcgatac gaaagccaaa atgcgcgtgc cgtgcgtgtt ctcaaccgaa aacatcacgc
     3661 cgccgcaatc gggcgtggcg tgttctgccg atgccgcgcc gcagggcttg tcgtgcgccg
     3721 gctatggcgc aagtatgctc gttaccgctt cgttcggtct atattgcgcg caggcggcgg
     3781 tggaacacat cgcgggcaaa aaataagcaa tgccgtctga aacaggattc agacggcatt
     3841 tgaacaaact atggttatga tttaagacaa caaaggatac ggataaaaaa taacataaaa
     3901 tatatgattc ctaataatat accaagtatc ggagagctat ttaatggaat tcgttaataa
     3961 tttagttatt ttttcatttt tattactaat gcttattccg atattttttg tagtatatgg
     4021 tatataccat aagatacgtt atcgcaaaat atatatccta agaataagtt ttacattatt
     4081 agtggtaata ctttgcagta tgtattacat atattgccgt tatcttgacc aacaaaaagt
     4141 agcttattat tgcatagatg aacaatgtat ttctattgtt catctataca aagattatgg
     4201 tataaactct cccacatatg cgagaattta cgcaggaaaa atattgttta gatttcaagt
     4261 aagagctaaa aattacgctg aattacttat ggaagatgat atatcaatta gtaaaaaaat
     4321 tttggggaat aaatttatca tttatgggtc gctacctgta atatacggta atgtagataa
     4381 tattgaagta aaagaagcta ctggttatat agatagagcc agtactgatt atattgtctc
     4441 aagaaactta aaattcagac atttatatta attaagaggt tttagcaaga ccgtattcac
     4501 aacctgctcc ttttcaaaac atttgcattt aaaagccgtt ataatgccgt ctgaacatct
     4561 gcccgaccac attatgcgtg aatgccggca gattgttttc ttttgtaaac ttatattaaa
     4621 atccacttac cgattcacgc catgccgccc atccctgccc catccgcacc atccgagcac
     4681 actgtcgcat gggtattcgg ccaacccgtt accgatttgc cccaggattt gtttattccg
     4741 cccgatgcat tgaaagtcgt attgggcagt tttcaaggcc ctttggatct actgctgtat
     4801 ctgatccgca agcagaacat cgatgttctc gatattccga tggtgaagat taccgagcag
     4861 tatctgcact acatcgccca aatagaaacc tatcagtttg atttggcggc ggaatatctt
     4921 ttgatggcag caatgctgat tgaaatcaaa tcgcgcctgc tgctgccgcg taccgaagcc
     4981 gtcgaagacg aagaagccga cccgcgtgcc gagttggtgc gccgcctgtt ggcttatgaa
     5041 cagatgaagc tggcggcaca agggttggat gcgcttcctc gtgcgggccg ggatttcgca
     5101 tgggcgtacc tacctctgga aattgccgtc gaagccaagc tgcccgaagt ctatattacc
     5161 gacttgacgc aagcgtggct gggtattttg tctcgggcaa aacatacgcg cagccacgaa
     5221 gttatcaaag aaaccatctc cgtgcgcgcg caaatgacgg caatcctgcg ccgtttgaac
     5281 ggacacggga tatgcaggtt tcacgacctg ttcaatcccg aacagggcgc ggcttacgtg
     5341 gtcgtcaact tcatctcact gttggagctt gccaaagaag gattggtcag aatcgtgcag
     5401 gaagtcggtt tcggagaaat ccgaatcagc ctcaatcatg agggggcgca ttcagacggc
     5461 atttccggca cacggggcgg gcgcgatgtg ttctaatacg ccccaagccg ccaccaaaaa
     5521 tcgggagaca cgccatatga ccggcatcat acattcgctg cttgacaccg acctctacaa
     5581 attcactatg ctgcaagtgg ttctgcacca gtttccgcag acgcacagcc tttacgaatt
     5641 ccgctgccgc aacgcctcga ccgtctatcc gcttgccgac atcaaggaag acttggaagc
     5701 cgaactcgac gcgctctgcc ggctgcgctt cacccacgac gaactcggct atctgcgctc
     5761 cctgcgtttc atcaaaagcg actttgtcga ttatctcgaa ctcttccagc tccaacgccg
     5821 ctttgtcgaa atcggcacag acgataaaga ccgtctgaac atccgtatcg aaggtccgat
     5881 gatacaggcg atgttttttg aaatcttcat cctcgccatt gtcaacgaac tttacttccg
     5941 ccggctggaa acccctgccg ttatcgaaga aggtgaacgc cggcttcaag ccaaagccgc
     6001 gcgcctcaaa gaaatcgccg ccgcacaaaa ccccgacgat ccgcccttcc tgatttccga
     6061 cttcggcacg cgccgccgct acaagctcgc gtggcaggaa cacgtcatcc gcaccctgct
     6121 cgaagccgcc ccaagcatcg tgcgcggcac cagcaatgtc tttctcgcca aaaaactcgg
     6181 catcaccccc atcggcacca tggcgcacga attcctccaa gccttccagg ccctcgacgt
     6241 acgcctgcgg aatttccaaa aggccgcgct cgaaagctgg gtgcacgaat accggggcga
     6301 tttgggcgtt gccctgaccg acgtggtcgg tatggatgcc ttcctgcgcg atttcgacct
     6361 ctatttcgcc aaacttttcg acgggctgcg ccacgacagc ggcgaccctt acgtttgggg
     6421 cgacaaagcc tacgcccact atcaaaagct caaaatcgac agccgcacca aaatgctgac
     6481 cttctccgac gggctggaca tcgaacgctc ttgggcatta caccaatatt tcaaagaccg
     6541 cttcaaaacc ggcttcggca tcggcaccaa cctcaccaac gatatggggc atacgccttt
     6601 gaatatcgtg ttgaaactgg tcgaatgcaa cgggcagtcc gtcgccaagc tgtccgactc
     6661 tccgggcaaa accatgacca acaacagcac cttcctcgcc tacctgcgcc aagtgttcga
     6721 cgtacccgaa cccgaaacgc cgtaaaccgg cagaaaaagc gcacaattcc tgtttctgcc
     6781 gcataaaatc ttttaaaata ccgcctgatt tgaatttaac cgaaagaccg aacttcatga
     6841 acctacatca aaccgtcgaa cgcgaagccg ccgccgcctt tgccgccgca ggcatcgccg
     6901 acagtcccgt tgttttgcag ccgaccaaaa atgccgaaca cggcgatttc caaatcaacg
     6961 gcgtgatggg tgcggcgaaa aaagccaaac aaaatccgcg cgaattggcg caaaaagtgg
     7021 ctgaagcatt ggcggacaat gccgtgattg aaagcgcgga agtagccggc ccgggcttta
     7081 tcaacctgcg tctgcgccct gatttcctcg cacaaaacat tcagacagcc ttgaacgaca
     7141 cgcgtttcgg tgtggcaaaa accgacaaac cgcaaactgt cgtcatcgac tattcctcgc
     7201 ccaatctggc caaagaaatg cacgtcggcc atctgcgttc cagcatcatc ggcgacagca
     7261 tttcgcgcgt attggaattt atgggcaaca ccgtcatccg ccaaaaccac gtcggcgact
     7321 ggggtacgca gttcggcatg ttggtcgctt atttggtcga gcagcaaaaa gacaatgccg
     7381 cgttcgagct ggcggatttg gagcagtttt accgcgccgc caaagtgcgc tttgacgaag
     7441 cccctgcctt tgccgacacc gcgcgcgaat acgttgtgaa gctgcaaggc ggcgatgaaa
     7501 ccgttttggc attgtggaaa cagtttgtcg atatttcgct ctcgcacgcc caagccgttt
     7561 acgacacgct gggcttgaag ctgcgccccg aagacgtggc gggcgaatcg aaatacaacg
     7621 acgatttgca gcccgtagtc gatgatttgg ttcaaaaagg tctggcagtc gaagacgacg
     7681 gcgcaaaagt cgtgttcttg gacgagttta aaaacaaaga aggcgaaccc gccgcattta
     7741 tcgtgcaaaa acaaggcggc ggcttccttt atgcttccac cgatttggcg tgtctgcgct
     7801 accgtgtcgg cacgcttcat gccgaccgcc tgctgtacgt cgtcgaccac cgccaagccc
     7861 tgcactttga gcaactcttt accacttcac gcaaagccgg ctatctgccg gaaaacgtcg
     7921 gcgcggcatt tgtcggcttc ggcacgatga tgggcaaaga cggcaaaccg ttcaaaacac
     7981 gcagcggcga caccgtgaaa ctggtcgatc tgctgaccga agccgtcgaa cgcgccaccg
     8041 ctttggtgaa agaaaaaaat cccgaattgg gtgcagacga agcggcaaaa ataggcaaaa
     8101 ccgtcggcat cggcgcggtc aaatacgccg acttgagcaa aaaccgcacc agcgattatg
     8161 tgttcgactg ggacgccatg ctctcgtttg aaggcaacac cgccccctat ctgcaatacg
     8221 cctacacccg cgtgcaaagc gtgttccgca aagcaggcga atgggacgcg gacgcgccaa
     8281 ccgttttgac cgaacctttg gaaaaacaac tggccgccga gcttttgaaa tttgaaaacg
     8341 tgctgcaaag cgtggcggac acggcgtatc cgcactacct cgccgcctac ctctatcaaa
     8401 tcgctacctt gttcagccgc ttctacgaag cctgcccgat tttgaaagcc gaaggcgcaa
     8461 gccgcaacag ccgcctgcaa ctggcgaagc tcaccggcga cacgctgaaa caaggcttgg
     8521 atttgctggg catcgatgtg ttggacgtaa tgtaaaaccg caccgcccga ttgcggacaa
     8581 cagccccgca atcaaccggc ggaccatcct tatccgaatc tgaaaaaagc ggcgcgatac
     8641 accgtatccg ccgcctccca aaatgcgaaa cagacaaacg ccaagcaagc aagcaagcaa
     8701 gcaagcaagc aagcaagcaa gcaagcaaaa aattataacc cccttcctgc cgacgcacgc
     8761 actttccgcg cggcgcgttc cccttttccc gctcctcaaa cccgcctttt cttcaggcag
     8821 ggtttcagcc cgcctccttt ccctgttttc cttccctcaa cacgcgtgcg cgccccctgc
     8881 cgcaccgtgc cgcactttcg cgcccggacg gcatcggtct gtcatccggt tctctgtttt
     8941 aaatacccct gtttcagaaa gaaattgcag atgtttcaac acacaggacg acacataaag
     9001 caccgcccta tgtgttgccc tgatttggaa ggggttgcgc ccctcccaaa taaagtctga
     9061 tcctgccgcc ccgaaggaca gatgtccgag tggcggggtt tcaaccgaaa aggaaatacg
     9121 atgaatattc acgccctgct ctccaaacaa tggacgctgc cgccattcct gccgaaacgg
     9181 ctgctgctgt ccctgctgat actgctggcc cccaatgcga tgttttgggt tttggcactg
     9241 ctgaccgcca ccgcccgccc gattgtcaat ttggactacc ttcccgccgc gctgctgatc
     9301 gccctgcctt ggcgtttcgt caaaattgcc ggcgtattgg cgttttggct ggcggttttg
     9361 tttgacgggc tgatgatggt gatccagctt ttccctttta tggacctcat cggcgccatc
     9421 aacctcgtcc ccttcatcct gaccgccccc accccttatc agataatgac cgggctgttg
     9481 ctgctgtata tgctggcgat gccgtttgtg ttgcagaaag ccgccgccaa aaccgacttc
     9541 cggcacattg ccgtctgcgc cgccgttgtg gcggcagccg gctatttcac cggccatttg
     9601 agttactacg accgggggcg gatggccaat atcttcggcg caaacaactt ctattacgcc
     9661 aaaagtcagg cgatgctcta caccgttagc cagaatgccg actttattac cgccggcctg
     9721 gtcgatcccg tcttcctccc cttgggcaat cagcagcgtg ccgccacgca tctgaacgag
     9781 ccgaaatctc aaaaaatcct ctttatcgtc gccgaatctt gggggctgcc ggccaatccc
     9841 gaacttcaaa acgccacttt tgccaaactg ctggcgcaaa aagaccgttt ttcggtttgg
     9901 gaaagcggca gttttccctt tatcggcgcg acggtcgaag gcgaaatgcg cgaactgtgt
     9961 gcctacggcg gtttgcgcgg gtttgcactg cgccgcgcgc ccgacgaaaa atttgcccgc
    10021 tgcctcccca accgtttgaa acaagaaggt tacgccacct ttgcgatgca cggcgcgggc
    10081 agttcgcttt acgaccgctt cagctggtat ccgagggcgg gctttcaaga aatcaaaacc
    10141 gccgaaaacc tgatcggtaa aaaaacctgt gccattttcg gcggcgtgtg cgacagcgag
    10201 ctgttcggcg aagtgtcggc atttttcaaa aaacacgaca agggactgtt ttactggatg
    10261 acgctgacca gccacgccga ctatcccgaa tccgacattt tcaaccacag gctcaaatgc
    10321 accgaatacg gcctgcccgc cgaaaccgac ctctgccgca atttcagcct gcacacccaa
    10381 ttcttcgacc aactggcgga tttaatccaa cgccccgaaa tgaaaggcgt ggaagtcatc
    10441 atcgtcggcg accatccgcc gcccgtcggt aacctcaatg aaaccttccg ctacctcaaa
    10501 caggggcacg tcgcctggct gcacttcaaa atcaaataac aacaatgccg tctgaacgca
    10561 ccaacagcct tcagacggca ttttgcagac agaccgaccc ttcaagccca cttttttcat
    10621 catctccgat aaattgct
//
LOCUS       DAWFBO010000064        10602 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFBO010000064 DAWFBO010000000
VERSION     DAWFBO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.064"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(159..1268)
                     /gene="mltB"
                     /locus_tag="WGO87_001140"
     CDS             complement(159..1268)
                     /gene="mltB"
                     /locus_tag="WGO87_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0392505.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1177..1428)
                     /locus_tag="WGO87_001141"
     CDS             complement(1177..1428)
                     /locus_tag="WGO87_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392506.1"
                     /translation="MPLDGIFGGGRQISGGAKGNARHSAKPPIAADTAFRAAPLRRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1469..2896)
                     /locus_tag="WGO87_001142"
     CDS             complement(1469..2896)
                     /locus_tag="WGO87_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0392507.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3082..6780
                     /gene="mfd"
                     /locus_tag="WGO87_001143"
     CDS             3082..6780
                     /gene="mfd"
                     /locus_tag="WGO87_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0392508.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHTLPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYMGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     NIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRF
                     TAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            6929..7312
                     /locus_tag="WGO87_001144"
     CDS             6929..7312
                     /locus_tag="WGO87_001144"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0392509.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7334..8176
                     /gene="kdsA"
                     /locus_tag="WGO87_001145"
     CDS             7334..8176
                     /gene="kdsA"
                     /locus_tag="WGO87_001145"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0392510.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCTHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPYQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEADNGKL
                     ILCERGSSFGYDNLVVDILGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8191..8631
                     /locus_tag="WGO87_001146"
     CDS             8191..8631
                     /locus_tag="WGO87_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0392511.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            8679..9965
                     /gene="eno"
                     /locus_tag="WGO87_001147"
     CDS             8679..9965
                     /gene="eno"
                     /locus_tag="WGO87_001147"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0392512.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYNLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            9980..10258
                     /gene="ftsB"
                     /locus_tag="WGO87_001148"
     CDS             9980..10258
                     /gene="ftsB"
                     /locus_tag="WGO87_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0392513.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10299..10589)
                     /locus_tag="WGO87_001149"
     CDS             complement(10299..10589)
                     /locus_tag="WGO87_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0392514.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         2542 a         3091 c         2707 g         2262 t
ORIGIN      
        1 atgcaaaaat gccgtccgaa ccgaaacgtg gttcagacgg cattttatag tggattaaca
       61 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcaaggcaa
      121 cgccgtactg gtttaaattt aatccactat aaaaaacatt acaatcccgg gccgccaagc
      181 gaattggcaa tgtccctgac cgccgttaca tacatccgac tgtgattgta ctgccatacc
      241 gtataaaaat tgttcaagcc caaataatat tcaaacacgc cgggtgcggt ttccagtttg
      301 aacaaaaccg ccttttcatc atctgcaagc tcttcgccgg ggatgatgcc gtacgccttt
      361 aaatccgcca ccgtccgcgt cagggcggtt ttttcgccaa tgattgcctg aacatccgca
      421 cccggcgcca atgttgcaga caccagtatt ttcccgcccg tgcgccaacc gtgctgcttc
      481 atataattgg caaccgatgc cgcgacatcg ccgatgttgc cccatatgtc ccgatgtccg
      541 tccccgtcat aatccaccgc ccatttccgg tagctcgaag gcataaattg cggcatcccc
      601 attgcgcccg catagctgcc tttaaaggcg aaaacatcgc cgccttcttc ttttgccagc
      661 tttaaaagct cgaccaattc tttttggaaa aacccggcgc ggcgggggta atcaaagcct
      721 aaggtcgcca atgcgtccgc cacacggaaa ctgcccgtat ttttgccgta attcgtttca
      781 atcccgataa ccgccacgat aagttcggca ggcacgccgt atttttgcgc cacatcatcg
      841 ataagcgcgc ggttttccgc ataaaaccgg cgcgcgccgc gaaatttcgc cttgcccgaa
      901 tttcccgtgc ggaacacata ccacggacgc gatgtggagg ggcggtgcat aatcttgacg
      961 atgtccgcct tgtaagccgc tttgtcaaaa aaatcctgcc attccgcccg ggaaaaatcc
     1021 cctttcccga cttcatcgtc cacaaaacgg cggacatttg cattggcggc aaacccgctg
     1081 tcggataccg gtacggctgc cgcgtcaaat acggctgccg cgtcaaacgc ggggcggctt
     1141 tcttttttca tttcatccgc gcggggggct tgggcttcat ttgcccgggg cgggcgtgcc
     1201 tccatcgccg tacaggcaga caaagccgcc aaacaaattg ccagcggcag tatttttctc
     1261 tttttcataa atatgttccg aacaaatagg gtaagtggga aagcggcgca agggcgcggc
     1321 gcgaaatgcg gtatccgccg caatcggcgg ctttgcagaa tgccgcgcgt tgccttttgc
     1381 gccgcccgaa atctgcctgc cgccgccgaa aatgccgtct aaaggcactt cccctttcag
     1441 acggcattgc ctgcgccgta tttgcccgct atccgcaata tcggcagtcc aatatgtctt
     1501 tgcggatatc gttcagcagg caggctgccg cgtcttcgtg ttccgcctgt acgaagacaa
     1561 agagtcgggc gatgattttg ccgataaaga ctttttgcag ggcgcaggcg tcggtcagcg
     1621 tgtgttcctg caggaagctg cggatgtctt cgggtaaggt gtaatcacgg gcaacggcgg
     1681 caaagcgggc atcggtttgc aggcggtcga gcagggtttg gtttttgtcc aacttcatgc
     1741 ctgcttcttt ttcttcggcg gtggcgcgga caaactggtc gtaaatttcg gcataaagca
     1801 tatcgttcgg gccttcaaaa atcgtgaagg ggcggatgtc gatagcgata ttgccggcgg
     1861 tgtgtccgcg ttcaaaaccc ttcgcgccca agagtttttg caacatttgc gcggcggcgt
     1921 aagtgtattc cgtggcgagg gttttgacga tgttcgcctc catcagctga tgggcgacgg
     1981 gggcaacagg cgaaacggaa tggcagacgt agcggtaaag aatctcggaa acctgatggc
     2041 ggcgccggat ttcgcggcgt tcgtaatcga cgaatctgat gtcgttgcgg acgtatcgtt
     2101 ccaggttttc aaggatgtat tccataatgc cgtgcgtcat gccgatcagt tgcaggcggc
     2161 tgcggataaa gatgttttgg aacgcgcgca aaccggcagc gtcgctctgg gagagtttca
     2221 tcacggcggt tgcaggcatt tcggcatcga tgcggttgac ggcgtaacgg acggcgcgca
     2281 agccttcgga tgcgagggtt tcgcagcgga tgtatgtttt ggggacgagc agcaggtcga
     2341 tgactttggc gagtttgccg tttttgcgtt ctttggcggc aacgaggagg aagtcgcttt
     2401 gcgagttgcc ctgccagtat tttgcggcgt tgacgtaaat ggtttgtccg tcggtatatt
     2461 cgtagtaaga ctgcatttct cgggcaatcg ccgcgccgga ggtttcgggt tcggtaacgc
     2521 ccaaaccacc gccctcgcct ttgaaaacca tgtccaaacc ctgtgcgatt tgcgcttcat
     2581 cgccgaactc ttgcagtggc tgcaacacca gcgcgccttc gatgccggta cgcagcgtaa
     2641 cgggcacgcc gtaatgcccc gcaatccgca agacttcctg aatttcaaac tggctgccct
     2701 tgcgcccgcc gtgttttttg tcgaggaagg gcaacagcaa acccgcctgc ttcaaggcaa
     2761 gccatttgtc ttcgggcagg tatcgcatca ggtcgatacc gtctgaaaaa atgcggcgga
     2821 atgcggattc gatgtgcttt aaaaaagcag ccgtgtccat agttgacggc tgcgcgctcg
     2881 gttcggtgtg tatcatcggc ttcctctgtc ggttcccatt aatcggcggc cggtcaaacc
     2941 gcctgccaca gtttagagtt gattttctaa actttaccac aaagtgcgcc gggcaacaat
     3001 ccgccgacct ttcagacggc atcgcgcccc cctcccgtgc taaaatgacc gtttgcatca
     3061 ctgtccgccg attgccgcac tatgacctac cccatcccca aaccccgtga aaaatcccgt
     3121 tggtccaatc tttcgcaagg ctcgctgccc ttggctttgg cgcgttatct gccgcataag
     3181 cggctcaagg tcgtgctgac ccaagatgcg gaacaggcgc tacgccttca gacggcatgg
     3241 cggtttttcc gtccgcacga cacggcggtg ttcctgccgg actgggaaac gctgccttac
     3301 gagcgttttt cgccgcatca ggatttggtg tcggaacggc tgtcggcgtt gtggcagatt
     3361 aagagcggcg cggcggacgt gttgttcgtg ccggttgcca cggcgatgca gaagctgccg
     3421 cccgtgccgt ttctggcagg gcgcacgttt tggctgaaaa cagggcagac tttggatata
     3481 ggccgtctga aaaccgattt ggtagatgcg ggctacaacc atgtttccca tgttgttgcg
     3541 gcgggcgagt ttgccgtgcg cggcggcatt gtcgatttgt tccccatggg cagcgaaatg
     3601 ccctatcgca tcgatttgtt tgacgatgaa atcgacagca tcaaaacctt tgatacggac
     3661 acgcagcgca ccatctcccc cgtttccgaa atccgcctgc tgccggcaca cgagttcccc
     3721 accgacagcg aggcgcaaaa gattttccgc agccgcttcc gagaagaagt cgacggtaat
     3781 ccgaacgatg cggctgtata caaagccgtc agcaacggcc atttcggtgc aggcgtagaa
     3841 tattatctgc cactgttttt tgaaaacgag ctggaaacgc tgtttgacta tatcggcgaa
     3901 gatgcgctct ttgtctcttt gggcgatgtt catgccgagg caaaccgctt ttggagcgac
     3961 gtcaaatcgc gttacgccat ggcgcagggc gacgaaacct atccgccttt gcttccacag
     4021 catttgtatc tctctgctga tgtttttgca ggccgtctga aaaactacgg acaagtgctg
     4081 cccgatgttt ccggcaagga acacaccctg cctgaccttg ccgtcaaccg ccaatccgac
     4141 gagccgttgc aggcattgaa ggattttcag acgacctttg acgggcggat tctgctgtgc
     4201 gccgaaagct tgggacggcg cgaaaccatg ctcggcttct tgcagcaaaa cggcttgaaa
     4261 gccaaacctg tgtccgactg gcagggcttt ttatcggcgc atgagccgct gatgattaca
     4321 gtggcgccgt tggcttacgg gtttaaactg ggcggactgc aatcgccgaa ccaacagcaa
     4381 actacttccg cctccgaagg agaaggcgat gcggttaccg accaaaccga attttccgca
     4441 tccgcaacaa accctctccc tagccctctc ccacaggaga gggaacaaag tgcagccgcc
     4501 gtttcagaca gtctgaaagc agccgccgtt tcaaccgaaa gcagcctgcc ccccggtaaa
     4561 agtaatctgc acgggcaaat ccaacagcaa cctgccccct cccccgtggg ggagggttgg
     4621 ggagagggca aagcagttgc cgctcaaagt gccatcgccg tcatcaccga atccgatctc
     4681 taccaatacg tcgcccgttc gcgcgtccac aaccgccgca agaaacacgc cgccgtttca
     4741 gacggtctgt tgcgcgacct tgccgaaatc aacatcggcg accccgtcgt tcacgaagaa
     4801 cacggcatcg ggcggtatat gggcttggta acgatggact tgggcggcga aaccaacgaa
     4861 atgatgttgc tcgaatacgc aggagaagcg cagctttatg tgcctgtttc acaactgcat
     4921 ttaatcagcc gctactccgg tcaggcgcat gaaaacattg ccctgcacaa gctcggcagt
     4981 ggcgcgtgga acaaggcgaa gcgcaaagcc gccgaaaagg cgcgcgatac cgccgccgag
     5041 ttgcttaacc tttacgcccg acgcgctgcc caatcgggac acaagtttga aatcaacgag
     5101 ttggattatc aggcgtttgc tgacggcttc ggctacgagg aaaccgaaga ccaggccgcc
     5161 gccatcgccg ccgtgattaa agatttgacg caagcgaagc cgatggatcg ccttgtgtgc
     5221 ggcgatgtcg gcttcggcaa aaccgaagtt gccctgcgcg ccgcgtttgt ggcggtgatg
     5281 ggcggcaaac aggtcgccgt actcgccccg accacgcttc tggtcgagca gcacgcgcaa
     5341 aactttgccg accgtttcgc cgatttccct gtgaaagtcg ctagcctttc gcgtttcaac
     5401 aacagcaaag ccactaaagc cgcgctggaa ggcatggcgg acggcacggt cgacatcgtt
     5461 atcggcacgc acaaactggt gcaggacgac atcaaattca aaaacttagg tttagtgatt
     5521 atcgacgaag aacaccgctt cggtgtgcgc caaaaagagc agctcaaacg cctgcgtgcc
     5581 aatgtcgaca tcctcaccat gaccgccacg ccgattccgc gtactttaag tatggcgttg
     5641 gaaggactgc gcgacttctc gctgattacc accgcgccca gccgccgcct cgccgtcaaa
     5701 acctttgtca aaccctttag cgaaggcagc gtgcgcgaag ccgtgttgcg cgaactcaaa
     5761 cgcggaggac aggtattttt cctgcacaat gaagtagata cgattgaaaa tatgcgcgag
     5821 cggctggaaa ccctgctgcc cgaagcccgc atcggcgtgg cgcacggaca actgcgcgag
     5881 cgcgagctgg aacaagtcat gcgcgacttt ttacagcaac gattcaacgt gttgctctgt
     5941 tccaccatca tcgaaaccgg tatcgacatt cccaacgcca acaccatcat catcaaccgc
     6001 gccgacaaat tcggactggc gcaactgcac cagcttcgcg ggcgcgtcgg acgcagccac
     6061 caccaagcct acgcctacct gctcacgccc gaatacatca ccaaagacgc agaaaaacgc
     6121 ctcgacgcca tcgcggcagc agacgaactc ggcgcaggtt tcaccctcgc catgcaggat
     6181 ttggaaatcc gcggcgcagg cgaaatcctc ggcgaagggc agtccggcga aatgatacag
     6241 gtcggcttca cgctctacac cgaaatgctc aaacaagccg tgcgcgatct caaaaaaggc
     6301 cgccaacccg acctcgacgc gccgctgggc atcaccaccg aaatcaaact gcacagcccc
     6361 gccctactgc ccgaaagcta ctgccccgac atccacgagc gtctcgtcct ctacaaacgc
     6421 ctcgccgtct gcgaaaccgt gcagcaaatc aacgccatac acgaagaact cgtcgaccgc
     6481 ttcggcctgc ccgaacaacc cgtcaaaacc cttatcgaaa gccaccacct gcggcttatg
     6541 gcaaaagaat tgggtatcga tgccattgat gcggccggcg aagcggtaac ggtaaccttc
     6601 ggtaaaaaca ataatgtcga tccaaccgaa atcatcctgc tgattcagaa cgacaaaaaa
     6661 taccgccttg ccggcgccga taagctgcgg tttaccgcag agatggaaaa tatcgaggtc
     6721 agaatcaaca ccgtaaaaaa cgttttaaaa accttgcaaa acagatgcct gcccaaataa
     6781 agccgacacc gcaatgccgt ctgaaacacc gttttccttg tccgaaagcc gccattatga
     6841 atttgaagga aactccacta taatacggca ttcagatttc cagacggcat cgcgcccgtc
     6901 agaccgcaca caaaccaaaa ggaaatacat gttccgtact atgcttggcg gaaaaatcca
     6961 ccgcgccacc gtaaccgaag ccgatttgaa ctacgtcggc agcattaccg tcgatcaaga
     7021 tctgttagac gcggcaggca tctaccccaa cgaaaaagtc gccatcgtca acaacaacaa
     7081 cggcgaacgt tttgaaacct ataccatttc aggtaaacgc ggcagcggcg tgatttgtct
     7141 gaacggtgct gcggcaaggc tggtgcagaa aggcgatatc gtcatcatca tgtcttacgt
     7201 ccaactctcc gaacccgaaa tcgccgcgca tgaacccaaa gtcgtcttgg tagacggaaa
     7261 caacaaaatc cgcgacatca tctcctacga gccgccgcac accgtgctgt aaccctgaaa
     7321 acggacattg atcatggata ttaaaattaa cggcattacc gtcggtaacg atgctccttt
     7381 cgtactgttc ggcggcatca acgttttaga agatttggat tccaccctcc aaacctgtac
     7441 gcattacgtc gaagttaccc gaaaactcgg tattccctat atctttaaag catctttcga
     7501 caaggcaaac cgttcctcca tccattctta tcgcggcgta ggcttggaag aaggcttaaa
     7561 gatttttgaa aaagtcaaag cggaattcgg catccccgtc attaccgacg tacacgaacc
     7621 ctatcagtgc caacccgtcg ccgaagtgtg cgatgtcatc cagcttcccg cattccttgc
     7681 gcggcagacc gatttagtgg ttgccatggc aaaaaccggc aacgtcgtca acatcaaaaa
     7741 acctcagttc ctcagcccct ctcaaatgaa aaacattgtg gaaaaattcc acgaagccga
     7801 caacgggaaa ctgattttat gcgaacgcgg cagcagcttc ggctacgaca acctcgttgt
     7861 cgatatactc ggtttcggcg tgatgaaaca gacttgcggc aacctgccgg ttattttcga
     7921 cgttacccat tccctgcaaa cccgcgatgc cggttctgcc gcatccggcg gtcgtcgcgc
     7981 acaggctttg gatttggcac ttgcaggcat ggcaacccgc cttgccggtc tgttcctcga
     8041 atcgcacccc gatccgaaac tggcaaaatg cgacggcccc agcgcgctgc cgctgcacct
     8101 tttagaagat tttttaatcc gcatcaaagc attggacgat ttaatcaaat cacaaccgat
     8161 tttaacaatc gagtaacacg gtttcgcctt atgatgcaga ctttccgaaa aatcagccgg
     8221 tatgtcgcaa ccttgtggct cggtatgcag attatggcgg gttatatcgc cgcaccggtg
     8281 ctgttcaaaa tgctgcccaa aatgcaggcg ggcgaaattg ccggcgtatt gttcgacatc
     8341 ctctcttgga gcgggcttgc cgtttggggc gcggtgttgg ctgccgcctt tgccatcctg
     8401 acccgactgc aaaccgccct gctgcttttt ttattgtccg cccttgccgc caaccaattt
     8461 ttggttaccc ccgttatcga ggcactgaaa tacgggcatg aaaattggct gttgtcgttt
     8521 gtaggcggat ccttcggaat gtggcacggt atttcaagca tgattttcat ggcaaccgcc
     8581 ctactttcag cagttttaag ttggcggctt tccggcaaag atgccgtctg aagccctccc
     8641 atttttttac ctcccttcac ttcacttgga gaacattcat gagcgcaatc gttgatattt
     8701 tcgcccgcga aattttggac tcacgcggca accccacagt cgagtgtgat gtattgctcg
     8761 aatccggcgt aatgggacgc gcagccgtac cgagcggcgc gtccaccggt caaaaagagg
     8821 ctttggaact tcgcgacggc gacaaatccc gttattcggg caagggcgta ttgaaggcgg
     8881 tcgaacacgt caacaaccaa atcgcccaag ccctcattgg tatcgatgcc aacgagcaat
     8941 cttatatcga ccaaatcatg atcgaattgg acggtactga aaacaaaggc aatttgggtg
     9001 cgaatgcgac tttggcggtt tctatggcgg ttgcacgcgc cgctgccgaa gactcaggcc
     9061 tgccgcttta ccgctacttg ggcggcgcag gcccgatgtc cctgcccgta ccgatgatga
     9121 acgtcatcaa cggcggcgaa cacgccaaca acagcctgaa catccaagag tttatgatta
     9181 tgcccgtcgg cgcaaaatct ttccgcgaag cgttgcgctg cggtgcggaa attttccacg
     9241 ccttgaaaaa actgtgcgac agcaaaggct tcccgaccac agtcggcgac gaaggcggtt
     9301 tcgcccccaa cctgaacagc cacaaagaag ccctgcaact gatggtcgag gcgaccgaag
     9361 ccgccggcta caaagcgggc gaagacgtat tattcgcatt ggactgcgcc tccagcgagt
     9421 tctacaaaga cggcaaatac aacttggaag ccgaaggccg ctcctacacc aacgcggaat
     9481 ttgccgaata tctggaaagt ctggtcaacg agttccccat catctccatc gaagacggca
     9541 tggatgaaaa cgactgggaa ggctggaaac tgctgaccga aaaactgggc ggcaaagtcc
     9601 aactcgttgg cgacgacctc ttcgttacca acccgaaaat ccttgccgaa ggcattgaaa
     9661 aaggcgtggc aaacgcacta ttggtcaaag tcaaccaaat cggtactttg agtgaaaccc
     9721 tgaaagccgt cgatctggca aaacgcaacc gctacgccag cgtgatgagc caccgctccg
     9781 gcgaaaccga agacagcacc attgccgact tggcagtcgc caccaactgt atgcagatca
     9841 aaaccggttc tttgagccgt tccgaccgca tggcgaaata caaccaactg ctgcgtatcg
     9901 aggaagaatt ggcggaagcc gccgactacc ccggcaaagc cgcattctac caactgggca
     9961 aataagaaaa ggttaaggta tgaagtgggt aactgtcgtt ttatccttcg cacttgtctg
    10021 ttgccaatac agcctctggt tcggcaaagg cagcatcgga cgcaacagca gtctgagaga
    10081 acagattgcc gttcaagaag aaaaaaacca gacactcgcc ctacgcaatc attcccttgc
    10141 cgccgaagtc tatgatttgg aaaacggtca agaagccatt tcggaaatcg cccgggtaga
    10201 actgggttat atccaagacg gtgaaacctt ttaccgactc atcaggcata accggtaata
    10261 ccgtcaaaaa gccgtccgaa ccaatgttcg gacggctttt atttcaacaa actgtcagac
    10321 agcccctcat cctcccccga caaaccgcaa tccagcctga catccccctc gacgcaacag
    10381 cagcacggca gtatctcgtc ccgccccaaa aaagccaaag gcggctcccg ataagtaacg
    10441 cttccctcca aaatcttcac ccggcacgat ccgcaatatc cgcttcggca ctgatattcc
    10501 accatatgcc ccgtccgttc caagccttcc aacagggtct cgccctccaa gagctcaaaa
    10561 aaacccttat tcgtaccaat gcgcgccatt tccgaccaat ca
//
LOCUS       DAWFBO010000065        10434 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFBO010000065 DAWFBO010000000
VERSION     DAWFBO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.065"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            140..322
                     /locus_tag="WGO87_001150"
     CDS             140..322
                     /locus_tag="WGO87_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392515.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(384..1385)
                     /locus_tag="WGO87_001151"
     CDS             complement(384..1385)
                     /locus_tag="WGO87_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0392516.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1462..5295)
                     /locus_tag="WGO87_001152"
     CDS             complement(1462..5295)
                     /locus_tag="WGO87_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0392517.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGNEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALIREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHNGVEFVELAGLDGKHPIIHCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKDDESCFIAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELADIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKVLRAAMIQTGFPAQNFAYKIGKLLPVGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5556..5652
                     /gene="ffs"
                     /locus_tag="WGO87_001153"
     ncRNA           5556..5652
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGO87_001153"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5820..7511
                     /gene="dld"
                     /locus_tag="WGO87_001154"
     CDS             5820..7511
                     /gene="dld"
                     /locus_tag="WGO87_001154"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0392518.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGRALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNQQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            7643..7750
                     /locus_tag="WGO87_001155"
                     /pseudo
     CDS             7643..7750
                     /locus_tag="WGO87_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7806..8177
                     /locus_tag="WGO87_001156"
     CDS             7806..8177
                     /locus_tag="WGO87_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0392519.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8332..8880
                     /locus_tag="WGO87_001157"
     CDS             8332..8880
                     /locus_tag="WGO87_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0392520.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            9010..10101
                     /locus_tag="WGO87_001158"
     CDS             9010..10101
                     /locus_tag="WGO87_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0392521.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGKVLAGTGMSNTVKHLAGIEKHNLQAERVEGGYTVNGALPWVSNIGEEHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLAGLAWSGTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT         2416 a         2875 c         2825 g         2318 t
ORIGIN      
        1 attttgcaaa ggtctctatt tgtactgtct tcggctcgcc gccttgtcct tatttaaatt
       61 taattcacta tatttgcccg tgcgtttgtg ataaattcgg cactgtattt gatatgattt
      121 taacaaatcg ggaggggcaa tgttttggta tgtaatcggc ttttgctcct ttgtcgtcgc
      181 gctgctgtcg ctgtgggtca atgtcggcgc attcggtatg caggaagacg ataccccgca
      241 atcggattat gaaaaacgtt tgggcttggg tgcgaagctg aaaaacaaaa atacgccgga
      301 tacggaagaa aagcagcaat aaagatatga aatgccgtct gaaagatttt cagacggcat
      361 ttgctttgaa gcatgggaga cagtcaaaca tttatctcat gctcaaatgc gttcaggatt
      421 cggcggactt catccgcatc cttaaccgtt cggacgcggt caaacagcgt ttgcgccggg
      481 tcgaacgttt ttttcatcat gcccaaccat tgcttaagac gggcgatcgg gtatttattg
      541 tttgcctctt ttgtcaggca cagctcgaaa aactgccgta tccatttgga aacttcggca
      601 aaatccgtgt ctttgaccgg cccgccgttc tcgtattgct tgatttgccg cgccaaatcg
      661 gggcggatga ccgcgccgcg accgagcatc acgctgttgc agccgctgat tgttttgatg
      721 ccgatatagt cttgcaggct gaaaacgtcg ccgttggcgg taacgggaat attgacgctg
      781 tctcggattt tccttatcca ttcccaatgc gccggcggtt cgtaaccctc ggctttggta
      841 cgcgcgtgta cggtcagtcc gcatgcgccc ccttcggcaa tcgcacaggc gcattccaaa
      901 gcccggcttt tgtcttcgta accgagccgc atttttgcgg tgagcggaat atgtgcgggc
      961 aaacgtccgc gcagcgtttt gacgatgtgg aatatcagtt ccggctcttt taaaaggatt
     1021 gcgccgcctt tgtgtttgtt gacggtgggc gcggggcagc cgaagttcaa atcgattttg
     1081 ttcgcaccga agcggacggc ttccagcgca ttcgccgcca tattgtccgc atcgctgccc
     1141 aaaagttgga cggtgcaggg cgtgccggaa aacgttttgt ttccgttggc gatttcgggg
     1201 acatatttta accatatgga tcgggaatgc acggtatggg taatgcgtac aaattcgctg
     1261 acgcattcgt cgtagccgcc aatacgcgtc agcaggtcgc gcatcacgtc atcgaccaga
     1321 ccctgcatag gggcaaggat gattcgggtt ttttgtttgg gttcggttgt ttgtccgtca
     1381 atcatgattc gggtaggtat ttgattcaaa caatgccgtc tgaaggcttt cggcgttcag
     1441 acggcatttc cgtgtccgtt gttacagcaa tactttttcc acgccgccgt tgttggcttt
     1501 tttgacgaac tcgtccagcc agttttcgcc gagaatgtgt ttcgccattt caatgactat
     1561 gtagtcggca ggcatattgt tgtcgtcggc gtagcggctc aagccttgca ggcaggcggg
     1621 gcaggaggtc agcattttga cgggttcgcc ctgcggcagc tctttgaggt ttttctcgat
     1681 ttcctcttgt ttgcggaact tgacctgagt ggcgatgtcg ggccgtttga cggcgaacat
     1741 accggactcg ccgcagcagc ggtcgcttaa gacgactttc tgccccatca ggctgctggc
     1801 catttgggtg gcgttcatgg ttttgatggg ggtgtggcag gggtcgtggt agaggtattg
     1861 ctgaccttgc acgccgtcga gtttcacgcc tttttcgagc aggtattcgt ggatgtcgat
     1921 gatgcggcag ccggggaaga tttcttcaaa gcggtatttt tcgagctggt cgtaacaagt
     1981 gccgcaactg acaacgacgg tcttaatgtc gaggtagttg agggtgtttg ccatacggtg
     2041 gaaagccacg cggttgttgg tgctcatttc ttcggcttta gccttgttgc cgcccgcgtc
     2101 ttgcggatag ccgcaacaca tatagccggg cggcaggacg gtttgtacgc cgacgtgcca
     2161 gagcatggct tgaacggcaa gtccgatttg gctgaacagg cgttccgaac cgcaacccgg
     2221 gaagtagaac acggcttcgg catcttcggg cgcggcgggg ttgcggatga tggggatgct
     2281 tttgccgtct tcgatgccca ataaggagcg cggtgttttg gcgggtacgt ttttgggcaa
     2341 aggacggttg atgaaatgga taacctgttc tttaattggg gctttgccga cggttgcctt
     2401 gggttcggct ttttgctttt tcgtgccgac gggcagcagt ttgccgattt tgtaggcgaa
     2461 attctgcgcg gggaagccgg tctgtatcat ggcggcgcga aggactttga tggttttcgg
     2521 gccggtcgcg tttaggaatg ccatacccat tgaagctgcc ggcgcaaagc gtttgtggcc
     2581 ggaatcggca aggtagttgc ggacggctac ggttacgtcg ccgaagtcga tattgacggg
     2641 gcagggtttg acgcagcggt ggcacacggt gcagtggtcg ccgatgtctg ccagttcttc
     2701 aaagtgtttg acggaaacgc cgcgccgggt ttgttcttcg tataagaagg cttcggttaa
     2761 cagccctacg ccgaggattt tgttgcgcgg actgtacagc aggttggcgc gcggaacgtg
     2821 ggtggagcag acgggtttgc atttgccgca gcgcaggcag tctttgacgg aatcggcaat
     2881 cgtgccgagg tttgattttt ccataatcag cgattccgcg cccaacagct cgaaggacgg
     2941 cgtgtaggcg ttgcgtaagt ccgagccttt catcagtttg tggcggttga aggtgtgttt
     3001 ggggtcgact tggtttttgt agtcccaaaa cggctgcaag tcttcatcgg tcaaaaattc
     3061 gagcttggtg atgccgatgc cgtgttcgcc cgaaatcacg ccgccgagcg aacgggcgat
     3121 tttcataatg cgttcgaccg aacggtaggc tgtctgaagc atttcggcat cgtctgagtt
     3181 gaccggaata ttggtgtgaa cgttaccgtc gccggcgtgc atatgcaggg cgacaaagac
     3241 gcggctgcgt acggttttgg cgtggatttt gcccaagcct tggataatct tggtatcggt
     3301 tttgccgctg aagatttcgg caagcggctt cattacgtct gctttgacag aaacgcgcag
     3361 gcggaaatcg cggaaagcaa taaagcaact ttcatcgtct ttggcttcgg gcgcggcgtg
     3421 gacggctgcg ccgtagcggg ctttgtagtc ggcaagcggc gtgtcgagat tggcgagcag
     3481 ccattcccaa cgcgttttga cggcggaaac gtgggctagg gcgtgtttgc cgcgttcgcc
     3541 caacagttcg gcggtcggca ggtcagtgcc cattttgtcg atggggagtt tgcccgaaag
     3601 atattgctcc aaggcggcac agagtttgag cttgttttgg atggaaagct caatgttgat
     3661 gcgttcgatg ccgtccgaat actcgccgag cctttcgagc gggatgacca cgtcttcgtt
     3721 aattttaaag gcgttggtgt gcttggcgat ggcggcggtg cggctgcggt cgagccaaaa
     3781 ggttttgcgc gcttcgggag acacggcgat aaagccttcg ccatcgcggg cgcgggcaag
     3841 ttcgcaaatg tgttcggcgg ctgcctctac ggcggcttcg tcgtcggaaa ccacgtccgc
     3901 cagcaagacc attttcggcc gtcccttgcc cgccgctttg gttgcgtagc cgacggcgcg
     3961 gacgtaacgc cagtccaaat gttccaaacc tgccagccga acgctgtcgt gggcgagcag
     4021 gaaatcgcgg atttcgacga tagaaggcgt agcggtggcg accgtgccga aaaactccat
     4081 acacacagtg cgcgtgtatt tcggcatttt gtgcaacacg aaagcaacgc cggtgatgat
     4141 gccgtccgtg ccttctttct gtacgccggg caggccgctc aagaatttgt cggtaacgtc
     4201 tttgcccaaa ccgactttgc gaaatttgtg gccgggaatt tccaaacgtt cggttttaac
     4261 gatattgagg ccgtctgaat ccagcgtgtg aacgtcgaac acggcggttt cttcgtcgtg
     4321 gattttgccg aaattgtggc gcacgcgctc gatgcgcagc cattcgccct gcgggttgac
     4381 catctgccag taggcgaggt tgtccaacgc cgtcccccac agcacggctt ttttaccgcc
     4441 cgcgttcatc gccacattac cgcccacaca ggacgcatcg gcggaagtcg gatcgacggc
     4501 gaacaccaaa cctgcctgat gcgcggtttc ttccacccgc cgcgttacca cgcccgcgcc
     4561 gcaatggata atcggatgtt tgccatccaa gcccgccagt tcgacaaact ccacgccatt
     4621 atgtttatcc agcttttccg tattgatgac cgcgctcatc gcgtccaaag gaatcgcgcc
     4681 gccggtataa cccgtaccgc cgccgcgcgg gataataacc aaatccagct cgattaaggc
     4741 gcgcaccaaa ggcgcgatct ctgcctcggt atcaggattg acgacgacaa acggatattc
     4801 gacgcgccag tcggttgcat cggtaacgtg cgttacccgc gccaatccgt caaacataat
     4861 attgtgcggc ttggtgattt tgctcaaacg ctccaaaatc tgccgccgct tttggctggt
     4921 ttcatcaaaa ctgctatcaa aacgctcgac tgctttttct gctgcggcaa ccaagacatc
     4981 cacttgccga ttatcgtcgc ggcgtttgcg gatttcgttc aggcggtggc gcatttcgcg
     5041 tatcaacgcg gcgcggcgtt tcggatgctc cagcaaatca tcgaccagat acggattgcg
     5101 cacgaccacc caaatatcgc ccaacacttc aaacagcatc cgcgccgaac gtccagtttt
     5161 acgctgtccg cgcaagtctt gcagaatctg ccacgcttcg ttgcccaaca gtcggatgac
     5221 gatttcgcgg tcggtataag aagtatagtt atagggaatt tcccgaatac gctgcggggc
     5281 ggtagtcgtg gtcatggtgt gtccgtgttt ggcagttttt atttgaatgc cgtctgaaaa
     5341 gtcggtttca ggcgagtttg aaaaggcgat aatgtagttc aatttttgat atttttcaat
     5401 gccgaaacag gaaataccct tatagaaaca aaataacgct ttgagaaatt gttgacaata
     5461 ttatcaaata taaattttag gtggtgtttc cggcgtatga ccgtatcaaa acggttgagt
     5521 ttttcagaaa acccgttata atgccgtttc tctgaggcgg gtctccccgc atggcaaatc
     5581 ggaacaccgg gtcaggggcg gaagccagca gcccactccg atgcgccagt gccgggggtt
     5641 tggcccgccg ccctatttga aacgccggag cttcgatgtt ccggcgttcc cgtatcgcac
     5701 cgatgccgtc tgaaagccgt tcagacggca ttttctttac tttgccataa cgtataacta
     5761 caatgggaaa acaatttcaa tgtgcatatg cacctgccga caattttaag gaaactgcca
     5821 tgagcgcatc acaattactg tcaagactga cccaaaccgt aggcgaaaaa tacatcatta
     5881 ccgaccccgc gaaaaccgaa caataccgcc aaggctaccg cttcggcgaa ggcagggcgt
     5941 tggcggtggt gcgccccgga agcattctgg aaatgtggaa aattctgcag gcgtgcgtcg
     6001 aagcagacgt gattgtgatt acgcaggcgg cgaataccgg actgaccggc ggttcgaccc
     6061 ccgacggcaa cgattacgac cgcgacatcg tgattgtgaa caccatgcgg atgaacatca
     6121 tccaaaccat caacaacaac caacaagtcg tctgcctgcc cggctcgacc ctgaaccagc
     6181 ttgaattgct gctgaaacct ttggggcgcg aaccgcattc ggtcatcggc tcatcctgta
     6241 tcggcgcgtc cgtcttgggc ggcgtgtgca acaactccgg cggcgcgttg gtacagcgcg
     6301 gcccggccta caccgaaatg gcgttgttcg cccaaatcaa cgaagaaggc aggttggaac
     6361 tggtcaacca cttgggcata gacttgggcg atacgccgga agaaatcctg accaacctgc
     6421 aaggtcatca ttatcaaaac aaagacatca aacaagacgc gggcaaagga cacgaccacg
     6481 cctattgcga acacgtccgc caagtggacg aaccgaccgc cgcgcgtttc aacgccgacc
     6541 ccgcgcgcca ttacgaagcc tcgggctgcg cgggcaaact gatggtgttc gccgtccgtt
     6601 tggacacctt cccgcaagaa aaacaaaccg ccgtgttcta catcggcacc aacgacatca
     6661 acgagctgac cgacatccgc cgcgccgcct tgggcgaatt tgaaagcctg cccgtttccg
     6721 gcgaatacat ccaccgccac gctttcgata ttgccgacgt gtacggcaaa gacacgtttt
     6781 acgtcatcaa aaaattcggt acgcaccaac tgccgaaatt atttgacttg aaggcgcgcg
     6841 tggacaggtt cggcaaaaaa gtcagcttcc tgcccaaaca tttttccgac aaggcaatgc
     6901 agttcgtcag caaattcctg cccgaccacc tgcccaaatc catgcgcgat taccgcgaca
     6961 aatacgaaca ccacctgatt ctgaaaatgg gcggaaaagg cgtggatgaa gcgcgcgcgt
     7021 tcttaaaaga atattttgca caccacggcg gcgcgttttt tgaatgcaac gccgaagaaa
     7081 cccaagccgc gatgctgcac cgttttgccg tcgcttccgc cgccatccgc taccgcgccg
     7141 tgcatgatga tgaagtggaa gatttggtcg cgctcgacat cgccctgcgc cgcgacgacc
     7201 gcgattggtt tgaaaaactg ccgccggaaa ttgacaataa aatcatccat aaattgtatt
     7261 acgggcattt tatgtgccac gttttccatc aggattacat catcaaaaaa ggcaacgact
     7321 gcatggcgtt ggaacacgaa atgctgcatc tcttagacca acgcggcgcg caatatcccg
     7381 ccgaacacaa cgtcggtcat ttgtatgaag ccaagcccgc gctcaaacag ttttaccgca
     7441 aactcgaccc gaccaacagc ttcaacccgg gtatcggcaa aaccagcaaa aagaaaaact
     7501 gggcggaata agcgcgtccg ctttgaaggc aggcaatgcc gtctgaaggc aaaacgccgt
     7561 tcagacggca ttgtcgggtt tgaagttccc cgtcgggtaa tcgctttcaa accgtatgcc
     7621 gacacacggg cgcgcccctc ccaaatatag tggattaaat ttaaatcagg acaaggcaac
     7681 gaagccgcag acagtacaga tagtacggca aggcgaggta acgccgtact ggtttaaatt
     7741 taatccacta taaagtctga tcctaccttc ccaaagggca gggtttcaac cgaaaaggaa
     7801 acacgatgaa accatacaaa atctacaccc atcccgccct gccgccccaa gccgtcaaac
     7861 aaggctggtc atggccgggg ctcttgttcg gcacgctgtg ggcgtgtttc aaaagaatgt
     7921 ggggtttggg gctggggctg acgggcgcga tattcgtgct ggctgtgttt gcccaactgg
     7981 tttacggcga cacgcccgcc acagactcgg cgtttaacgt attgggcttg gcggtttccg
     8041 tctggttcgg cgcaaagggc aacagccttt atgcacgcca ccttttatcg cgcggctata
     8101 ccgaattgcc cgaaacggtt gaggcagcca atccccaagc cgcattggcg caatatttcg
     8161 ggcgcggggg ccgataagcg cgttttcttt tccatcgtgc cgtctgaaaa atgttcagac
     8221 ggcatttctt ataacctttc gtttgcagca cataaccaat cagtctttcc tattccgttc
     8281 cgaaatgcat accatgcgcc cgtacccaat atttgcgaac aaggaaagaa aatggcacgt
     8341 ttaaccgtac acaccctcga aaccgccccc gaagccgcca aagcgcgcgt cgaggcggta
     8401 cttcaaaaca acggctttat ccccaacctt atcggcgtat tatcaaacgc ccccgaagcc
     8461 ttggcgtttt accaagaagt cggcaagctc aacgccgcca acagcctgac cgccggcgaa
     8521 gtcgaagtaa tccagattat tgccgcccgc accaaccaat gcggcttctg cgtggcaggg
     8581 cacaccaaac tcgcaaccct gaaaaaactc ctttccgaac aatccgtcaa agccgcgcgc
     8641 gctttggcgg caggcgaatt tgacgatgct aaactcggcg cgctcgccgc ctttacccaa
     8701 gccgtaatgg caaaaaaagg cgcggtatcc gacgaggaac tcaaagcatt ttttgatgcg
     8761 ggctacaacc agcagcaggc agtcgaagtc gtgatgggcg tagccttggc aactttgtgc
     8821 aactacgtca acaacctcgc ccaaaccgaa atcaaccccg aattgcaggc ttacgcctga
     8881 tacggcaaac cgcccgaata tcgggcggtt tttcaacccc cctcaaacaa aaacaagccg
     8941 ccaaacggca gggaagcagc cgcgcatcag gcgtgcgccc gatttccccg caaaccttaa
     9001 ggaacaaaga tgaacgccca aaccctgatt gccaacgttg ccgaactcgt caaaaccaag
     9061 ctcaaaccca tagtggacga catcgaccgc aaaggatact accccgaagc atttatgcgc
     9121 gaactcggcg caatcggcgg attcggcgca gtcggcacag aagccgaagg cggcaacggc
     9181 ttgggtttgg caacgcaaat cgccgtattg cgcgaaatcg gcaaagaatg cggcgcaacc
     9241 tccttcagcg cgtggtgtca ggcggcttgc gcgtggtatc tgcatcaaac gcccaaccaa
     9301 gccgtcaaag acaaatacct cgccgacatc ctgcaaggca aagtattggc aggcaccgga
     9361 atgtccaata ccgtcaaaca ccttgccggc attgaaaaac acaaccttca agccgagcgc
     9421 gtggagggcg gttatacagt caacggcgcg ctgccgtggg tatccaacat cggcgaagag
     9481 catatttggg caaataccgc ccaaatcggc ggcggctacg tcatgtttat caccggcgga
     9541 caatgggaag gcgtaagcct gcaaaactgc cccgaatttt gcgccctcga aggcacgcgc
     9601 accttcagcc tgaacttcaa agacgtattt atccccgacg aagacatcat cgccgcgccc
     9661 gaacagtttg ccgactacat ccaaagcatc aaagcaggct ttatcctcct gcaaatcggc
     9721 atcggcgcgg gcgtgataga cggcagcctc ggcatcatcc gcctcgccaa catcgtcaac
     9781 gccgaagtca acagctatct cgacgacggc tacgacagcc tcaaagcaag gctggacggc
     9841 gcgtgggcag aaaccgaacg gctcgccgga ttggcttgga gcggcacgcc cgacaacctt
     9901 gctaccctca agttgcgcga agccgccgcc gtactggcac ttgccgccgc ccaatccgcc
     9961 gccctgcatt ccggcgcaaa aggctacctg atgcgcagtc ccgcccaaag gcgcgtccgc
    10021 gaagcaatgt ttgtcgccat cgtaaccccc gcgatcaaac acctccgaaa agaaatagcg
    10081 gcaatcgaag ccgcaaaata aacaggcgga aaaaaccgaa acccccaccg cgtcattccc
    10141 gcgaaagcgg gaatccagcc cccaacgcaa caggaatcca tcggaaaaaa cagaaacccc
    10201 caaaccgtca ttcccgcaaa agcgggaatc cagcccccta acgcaacagg aatccatcga
    10261 aaaaaccgaa acctccaaac cgtcattccc acggaagtgg gaatccagta accgaaaaac
    10321 cacaggaatc tatcgaaaaa aaccccaccc cccaaaaaaa ccgggcggat acctcaccat
    10381 cctcccaaac cctgatttat agcggatgaa caaaaatcag ggcaaggcgg cgaa
//
LOCUS       DAWFBO010000066        10284 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFBO010000066 DAWFBO010000000
VERSION     DAWFBO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.066"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(92..1234)
                     /locus_tag="WGO87_001159"
     CDS             complement(92..1234)
                     /locus_tag="WGO87_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0392522.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQVNPAFAPISGSDNDRRIVQTALDNARSA
                     EVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1283..1705)
                     /locus_tag="WGO87_001160"
     CDS             complement(1283..1705)
                     /locus_tag="WGO87_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392523.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1698..2507)
                     /locus_tag="WGO87_001161"
     CDS             complement(1698..2507)
                     /locus_tag="WGO87_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0392524.1"
                     /translation="MTRDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2581..2958)
                     /locus_tag="WGO87_001162"
     CDS             complement(2581..2958)
                     /locus_tag="WGO87_001162"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0392525.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYAPALSEWLEEQG
                     IRHVNLSMADEGDTVLAFAVAEK"
     gene            complement(3158..3925)
                     /locus_tag="WGO87_001163"
     CDS             complement(3158..3925)
                     /locus_tag="WGO87_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0392526.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3935..4714)
                     /locus_tag="WGO87_001164"
     CDS             complement(3935..4714)
                     /locus_tag="WGO87_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0392527.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQSIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFLDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPA
                     SFDKKQGVRWGNILSVNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLVNVIKNAS
                     DIQEFSAQDDDLIIIDNVNGLHAGTDYTDKNRHYIRARITV"
     gene            complement(4713..4820)
                     /locus_tag="WGO87_001165"
                     /pseudo
     CDS             complement(4713..4820)
                     /locus_tag="WGO87_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4866..5594)
                     /gene="pdxJ"
                     /locus_tag="WGO87_001166"
     CDS             complement(4866..5594)
                     /gene="pdxJ"
                     /locus_tag="WGO87_001166"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0392528.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5647..6390)
                     /gene="recO"
                     /locus_tag="WGO87_001167"
     CDS             complement(5647..6390)
                     /gene="recO"
                     /locus_tag="WGO87_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0392529.1"
                     /translation="MSEHRINHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(6424..7551)
                     /gene="pheA"
                     /locus_tag="WGO87_001168"
     CDS             complement(6424..7551)
                     /gene="pheA"
                     /locus_tag="WGO87_001168"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0392530.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7590..8822)
                     /locus_tag="WGO87_001169"
     CDS             complement(7590..8822)
                     /locus_tag="WGO87_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0392531.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(9342..10196)
                     /locus_tag="WGO87_001170"
     CDS             complement(9342..10196)
                     /locus_tag="WGO87_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0392532.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         2472 a         2781 c         2660 g         2371 t
ORIGIN      
        1 atcctgattt ttgttaatcc actataatat cggccggata ttaaaaaaag ccgtctgaac
       61 atttttcaga cggcattttt gtacactttc ctcaacggtt cgatccctgc accatatcaa
      121 tcaggaacag gcggcaatcg aggttgccgt tataaagcgg aattttccgc ttgggcgaca
      181 ggcgcatgaa tttgggcatt tccctgtcgc cggtaaacat accgacccgc cagccggcat
      241 agtgtttttt caaccacgtc cccaactgcg gataaagtgc ctgcaaagcg cggacttcct
      301 caaggcgcac accgtagggc ggattggaca ccatgatgcc gttttcgccg ttcggacgga
      361 cggattgcgc gtcggcaacg ctgaaggaaa cgatgtcatc cacttcggca ctgcgtgcgt
      421 tgtccaatgc cgtctgaacg atgcggcggt cgttgtcgct gcccgaaatc ggtgcaaaag
      481 cggggttgac ctgcgcttcg gcgcggcggc gcaaatccgc ccacagcgtt ttatcgaaat
      541 tttgcagttt ttcaaaaccg aagcggcgca tcatacccgg tgcgcggccg gcggcaatcc
      601 aagcggcttc gatagcaatc gtgccgctgc cgcaaaacgg gtcttgaaac ggctgcgtgc
      661 cgtcgtagcc tgccgagagc agcaatccgg cggcaaggtt ttcgcgcagc ggggcttcgc
      721 cggtatcgag acggtagccg cgtttgaaca gggcttcgcc cgaagtgtcg atgaagattt
      781 cgacattgcg ttcgtccaaa aaggcgtgga tgcgaacatc gggcgcggct ttgtccacgc
      841 tcggacgtgc gtcgtaaatg tcgcggaaag cgtcgcagac ggcatctttg acggtcaaac
      901 caacaaattg gatgctctta acgttggcac gctttgcctc gactttgact ttgaacgtct
      961 gctgtaaagt aaaccaatta aaccagttga tatttttggc gagtttgtag atgtcgcgct
     1021 cattgcggta tgtccctttg gtcaggcgca gcaggatacg gctggcagta cgcgaatgca
     1081 ggttggcggc gtaaacctgt tccaatccgc cccggcagga aacgccgccg tcaaacactt
     1141 gtacatcggt acagccgagg ctttcgagtt cttgagataa aacggtctcc aagccgcgcg
     1201 ggcaggtggc gaaaagtgta tataaagtat tcataacgtg tcctttccgg acggatgttc
     1261 cggcaatggc ggattgaaag gtttattcgg cggtaaatgc cgtctgaaat gtccgactgc
     1321 cgattgtaat gtccgccgta aaatcgcgcc tgccttcgac acagatgggc aggcggatgc
     1381 gtactgcctg ccaagtcccc gacggttgcc gctcgaacat atagcggttg aaacccatat
     1441 ccatattttt catactgaag ctgatgctga cctgttccgt gccggcgggc gcgtgttcga
     1501 tataaatatc aaacggtttt ttggttgaaa cggcggcggc gcggacgcgg cttccgtccg
     1561 gcagcgtgca accctcggtc aaatcgcatt gcgccgccac agcttgcggc tgctgcgcct
     1621 gccaccattg caacaaaacg agcttgacgg cggcaaaggc aatcagcagc agtgcggcaa
     1681 gcagtaattt acggttttta ttcatgattc ttccctattg tttcatccct taccaccacg
     1741 tgcgccccca atccgagcca tagttttcca taacgtgcaa ccgtctgtcc gtttgctgca
     1801 agtacgagcg gcacggatac gccctgccgc agggcttctg ccgcctcttg tgcctgaaca
     1861 tcatcgcaca cgcgccaaac gaccgcatcc gcgccctttt cctgcgcccg ccgccattgt
     1921 tcgcgggtct gcaccaccat ccacacgctg tcggcgtgtt cgggtctgtc ctgccattgc
     1981 gccgcctcca tcaaaacgtt cgcaccgctt ccctctgccg aacccaaagg caggacgcgg
     2041 tacgcgccca tactgttttc tccgtgcaaa cccgttttca ggctgccgta caaacggcgc
     2101 gggacggaca gcgaacgcaa aagcgcgccg ttggcgggca gcatgggggc aacggtaaaa
     2161 tcacccgcct tctgccaaga ccattcctgc ccttcgcggg attgcggttt gcccgtccat
     2221 tggtcggggt tgacccatag gaatttcagg cagacgcggg cgtgttcgta ggaatggatt
     2281 ttggtcagcc aaggcgtggc ggcgaggatg cggatgccga gttcttcttc aaactcgcgt
     2341 tgcaaggctt ggaagtcggt ttcgcccgct tcgaccttgc cgccggcaaa ttcccaatat
     2401 ccggcatagg gtttgccttc ggggcgcgag ctgagcaggt agttgccgtc tgaatcgagc
     2461 aggatgccgg caacgacgcg gataaggggt cgggtgtctc gggtcatagg aaagatggga
     2521 aaagtttttc cgaacattag aaaagggcgg aacggtcaat ccggcccggc ggctgccgcc
     2581 ttatttttcg gcaacggcaa acgccaatac cgtatcgcct tcatccgcca tactcaaatt
     2641 aacgtggcgt atgccttgtt cctccagcca ttcggacagg gcgggggcgt agaaaaattc
     2701 cggtttgccc aacgcgtcgt gcccgatgcc gatgttgcgg aaggaaaccg caccgcgtat
     2761 gcccgtgccg acggctttgg cgaaggcttc tttggcggca aagcgtttgg caaggtagtt
     2821 gacgggtttg cccgcttgcg gaaattcaag cagctcttcc ggagtgagga tgcgcccggc
     2881 aaacgcctgt ccgaattttt tgtttaagcg gatgatgcgc ttgagggaaa caatgtctgt
     2941 gccgatgccg taaatcatat ttgcgctcct tcgcccttgg ttgcgggtag tgatgaatgt
     3001 gatgatgaat gccgtcaatt tcttggggcg ttaaaatggc gtgaatatcc acttttgggg
     3061 ttttgtcggt aacaattttc actgtaacct tttgcatttg aaattctaaa acatcgtgcc
     3121 aattatttaa taaggagtta aggcatttaa taatgaatta aagtttgatt aaacccgaat
     3181 gcccgatttt cagagccaat accaataaaa caataaaaat taacatttta aaaaaatttg
     3241 ggctaatctt agtccttaac cgaattccaa catacaatcc aataacagac aatacggaca
     3301 gtaaaaatat taaaccgtat tcactcttat ttaataacca atactggtct cttagcatat
     3361 atatttgaac aattttcgcc aaaagatagc atagattgct tgattttacg atacgatttt
     3421 tattttctgt ttcgctaagc aaaaatatta acaatatggg agacatggca ttggttgaac
     3481 cgccgatgat gcctgccaaa aacccaaaaa gaaccatatt cttattattg gcaactactt
     3541 gaatattttt tgcttttgca catacattta aaataccatt gacagaataa tacaatgtaa
     3601 tgattgccat cagtaaaagc agccaagaca ctggaagtat caaaagcaac ttcaccccca
     3661 aaatgctgcc aacgacgctg ccgatagcaa gcaatttata ggtttttaaa taataaacaa
     3721 tctcttgcca aaaacccttt ttgttattgc tgcatagaac caacaagctc attaacaggc
     3781 ttggtaatgc caccaaggca acaaccttag acaatggcat gttaaaagcc aatgcggttg
     3841 taccgagcat cggaaatccc atgcctgtaa ttccgtgcag tattgcggca gcaacaaaaa
     3901 cgatagattg cattatttct tgcataaccg tcctttatac agtaattctt gctctaatat
     3961 aatgcctgtt tttatccgta taatcagttc cggcatgcaa accattgaca ttgtcaataa
     4021 taatcaaatc atcatcttgt gcagaaaatt cttgaatatc actcgcattt tttataacat
     4081 ttacaagata atcaagtgca agtaccattt cctttgatac tttatttcta ttttcttcaa
     4141 tacctttata gatacaatca cttctaaatc taatcatttg agtattgacc gataagatat
     4201 taccccatct cacaccttgt tttttatcaa atgatgctgg tgttttaaat ggatataaat
     4261 tgcccgttaa tgtttttaag tgttttttac cggtttctgt tttagataac tgattgacaa
     4321 tatctgatga acttaaaaat agggaattac ctccatcatt ggctgatttt actacataca
     4381 taaccaaata actttccggg ttttcactaa aagatgaatc actatgaagt ggacattctc
     4441 caacatcttc cgagaaagtt atatcattat ttattacata gtcatcgccg cgatatttaa
     4501 tatccaaaaa tatagattta aaaccatcgt ctttgtggtt tgtcagcatt cctaattgag
     4561 atagaaagtc aaataatttc ttgtttctcc ggctgtcatc aagatggttt agattaagac
     4621 cgcgaacaat acaataccca atactctgta ctgtattgtg aatttcttct atcaaactat
     4681 tattgtttgc agataaaata tacttttgat tcatatagtg aattaaattt aaaccagtac
     4741 ggcgttggct tgccttgccg tactatttgt actgtctgcg gcttcgccgc cttgtcctga
     4801 tttaaattta atccactata acatatccct gtcgtgaaat gctgtctgaa agggtttgcc
     4861 tgcccttacg gcagcagcct tgccctgaac atcgcctcct tcatctggcg cacggcttcg
     4921 ggcagtccga ggaagagggc ttgggcaatc agcgaatgcc cgatgttcag ttcgcggatg
     4981 gcgaggattt gggcgatggg ggtaacgttg tgtatggtca gtccgtgtcc ggcgttgacg
     5041 accaagccca aatcgccggc aaaatgcgcg ccgttttgca gacgctcgaa ttgcttgagt
     5101 tgttcggcgt gactgcgtgc atcggcatat gcgccggtgt gcagctcgac aacgggcgcg
     5161 ccgacatcat aggcggcttg gatttgcctg tcgtcggcat cgataaacaa agacacgcgt
     5221 atgcccgcgt cggtcaggat tttggtgaat tcggcgattt tttcctgttg cgccaatacg
     5281 tccaaaccgc cttcggtcgt gatttcctgc cgtttttcag gcacgatgca cacgtcttcc
     5341 ggcatcactt taagcgcgtt ttcgagcatt tcttccgtca acgccatttc aaggttcagg
     5401 cgcgtgcgga tggcgttttt gacggcaaac acgtccgcgt ctttgatgtg gcggcggtct
     5461 tcgcgcaggt gcatggtaat caggtcggca ccgtgcgttt cggcaaccag tgccgcctcc
     5521 acggggctgg gataagtcgt gccgcgcgcg ttgcggacgg tggcgatgtg gtcgatgttg
     5581 acacccaaaa gcataatgtt tccttttctc ttcgtttttc tgtttggcgg catggtgtgg
     5641 gcgcagctac gcggtattgc ggtcaaactg ccttatctgt tccaacacct gccgcgattt
     5701 caagccttcg ggaagcagcg tgccgatcag cagccgtgtg attttcaatg cctgttgcag
     5761 gctttcggcg gtgcggaaac tgccttcgcg caaatcgatc aggctttgcc cttcgacaat
     5821 tgcgccggcc tcgtgcgaaa gcgcgtctgc gtcctcgcat acgggcatga ccgcctcttc
     5881 gggcatcagc cggtaggttt tgtccgccaa aatatcgccg cccgtgccgt cggcgtgcaa
     5941 atcgggggca acgcccaagg cattgagcag cttccactcg aagcggcgca aatcggcaat
     6001 gtggttggct tcacgacaca ccgcctccat cacttttgcc aacgcgtcgt acagttcggg
     6061 catcggatct tcgcgcgcgg tcagtttcaa caccagttcg ttcacataca gaccgctgaa
     6121 caacgccctg ccctgcggct gccgccatcc gccgacccat tcggcacggt gaagggtttt
     6181 gagttcctga ctgccgtacc acgacgcact gacgggcacg aaaggaacca atacgccgcg
     6241 caattcgctc tgccgcttgc gcgcgcttct tgccaaaagt gccacacgcc cgtaacggcg
     6301 gctgaaggct tcgacccgca ggctgctttc gcgccagggc gatgatgcca acaggaagac
     6361 gggttcgtgg ttgatgcggt gttcggacat catgcctgaa atgccgtctg aacgctgccg
     6421 ccgctacaaa acggcggtcg ggtacgaacc gatgactttg acgaacgaag cgcgttcgcc
     6481 caagcgttcc aatgccgtct gaatctgcgc gtcccggcgg tgtccttcga tgtcgatgaa
     6541 gaacaggtat tcccacaaaa cggatttgct cggacggctc tcaaacttgg tcatggaaat
     6601 acccgactcg gtcagcggtt gcagcagcga ggcaaccgca ccggcgcggt tgggcgcgga
     6661 aacggccagc gaagtcttgt cgctgctgct tgcaccggtt tcgtgatgtc ccatcaccaa
     6721 gaagcgcgtg gtgttgttcg gttcgtcttc gatgcactcg gcaaccatat cgagtccgta
     6781 gatttccgcc gccgtgcgtc cggcgatggc ggcaaccgta ccgtcgtccg attcggcaac
     6841 cagccttgcg gcttcggcat tgctggacac ggcaatccgt tcggcgttgg gcaggtgtct
     6901 gcccaaccag tcgttgcact gcgccaacgc ctgcgcgtgg gaaaagactt tggcaatgcc
     6961 ttcggtgctg ccgttgtttt tacgcaaaag gttgtggtgg atgcgcaaaa cgacttcgcc
     7021 gcacgcctgc aacgcggtaa cggcaagcaa atccaacgtg cgcccgaccg agccttcggt
     7081 cgagttttcc acgggggcga ccagatagtc tgcctgacgg gtttcaacct gcttgaagca
     7141 gttgtctatg gtcggacacg ccatggtgtg cgcggcgtgt ccgaaatgct taatcgccgc
     7201 ctgctgggta aacgtgccct gcggccccag ataggcgatg gtcagcgggc gttcgacggc
     7261 gaggcactcg ctcatcactt cccgaaacag gcgtgctacc gattcgtcgg gcagcgggcc
     7321 tttgttcaaa tcctgaatgc ggcgcaacac ggcgacttcg cgttcggggc ggtacactgc
     7381 gcccgtgcct ttcagctcgc cgatggcgtg ggcgtgttgc gcacgttcgt tgagcaggcg
     7441 caggatttcg gcatcgatgg tgtcgatggc gttgcggtgg ggaaggagga gttcgtcgat
     7501 agtttgggac ataatttcgc ctgaacagta atttatcgtg cattctaaca tcaaatgccg
     7561 tctgaaacgg tttcagacgg cattttccgt taaaggtatt cgctctgttc gttttccttc
     7621 cacgcccggt gcgaacacag ccacagaagc gcaatgccgc aagaggtgga cgcggtcatc
     7681 gttgccgcca tcacggttgc cgaaccgtcg tgcaagaagg tcgccgccat acccaccccc
     7741 gcgccgatta aggactggaa cacgcccaac acggcgttcg cgctgccgcc ctcttctttg
     7801 aaataagaca taaagcatgc ctgcgtgttc gcgccgacca agccctgcgt gccgacggaa
     7861 aacatcacgc acgcaaccaa gagccagaac ggaggcaacc cgaaaaacag cacggcggcg
     7921 agttgggaca ggttggcggc aaattggacg acaatccccc acagcaggat gctttgcgga
     7981 tgcgcgccgg ttttgagccg ccacgcggta atgcggttga aaaacatcat cgtgatgatg
     8041 ttgagtgcaa acgcccaagc gtattgatga ggcgtaacac ggtaaagctg ctggtacacg
     8101 aaggaagatt cggtcagaaa ggcgaacatc gaaccgaagc tgaatgcctg aaaaaacaga
     8161 taacccatcg cggcacgggt tttcaatacg cgtttgaacc gcccagccac cagcccgaac
     8221 acatcccgtc cgattttgcc gccgacggca ggcttgggca ggaaatactg caccaaaccg
     8281 agcagcacca gcgaatacgc cgccagaaaa acaaaaatcg cctgccagcc acccaagccc
     8341 tgcaacagtg cgccgaccat gggtgcgacc agcggcacaa ccatcaaaat gatgccgata
     8401 agggcaaaca tctgggcggc tttgcgtccg gaataataat cgcgcaccat tgcgccgacg
     8461 atgaccacag tcatgcccgc accgaatgcc tgcacgacgc gcaggttgag gagctgttcg
     8521 gcactcgaag caaatacgat ggcggcaacg gcaaggcagt agacggccag tccggtcagc
     8581 gcgacgggtt tgcgcccttt gatgtcggac accgaaccgc cgaccacctg tccgaacgcc
     8641 gtgccgaaca taaacaaact caggctctgt tcgatgcggt ggacatccgc gttcagcgac
     8701 tgcgccattt cgggaatcgc gggcaggtag gcatcgatgg aaaacggcat cagcgtaacc
     8761 agcatcgcca tcaaaaccgc catcagtttt tcgctcattt caggataatg ggcagaaggc
     8821 ataaattctc gtccgattcg caaacaaacc gcccggcggc ggaaaacaca atacgcggaa
     8881 acggaaaatt ttaacacacc ggcacaaaca ggcggcaaca aaagccccgg caaaaccgga
     8941 cgaaccggaa cgatatagtg gattaacaaa aaccagtaca gcgttgcctt gccttagctc
     9001 aaagagaacg attctctaag gtgcttaagc acccagtgaa tcggttccgt actatctgta
     9061 ctgtctgcgg cttcgtcgtc ttgtcctgat ttttgttaat ccgctataaa atcccgtcat
     9121 tcctgcgcag gcgggaatcc ggacatccaa cgctgcagca atttgtcgga aataaccgaa
     9181 actcaaaaaa ccggatgccc gcctgcgcgg gaatgacgat gcaaagatgg tcgttatttc
     9241 ggataaatat ccgtcttttc gttccggata cccgctttcg caggaatgga gatggaaaga
     9301 tttcagccta ttcggcaatc atcaaatacc gcttcccaac tttatttttg atgcgacagc
     9361 cggccggcaa ccaaggtggc ggcgatgacg acaaacacgc ccaaggcgga cacgggcgac
     9421 aggtgttcgc ccaaaatcaa aaccgccagc agcacgccga cgacgggttc gagcgaaatc
     9481 aacagtcccg aaacgttggc aggaacacgg ctcatcccct tgttccacag ccaataggcg
     9541 taccagccgc accccaaacc caaatacagc agcgacaata ccatcccgac gctccagtcc
     9601 acggtataac tttgcgccaa agcaagcgaa aacggcaggc acatcaacga tgcggcggca
     9661 atggaaacag atgtgaatgc cggtgcgccg atgcgtgcaa tcagcctttg cgtcggacgc
     9721 atagcggcac aaaagcccgc gcccgccaac aacaccagca ggcagccgaa ccagccgact
     9781 tcgccgccct cttccgcacc gcccgccatc agcagcgcga caccggcaaa tgccgccgcg
     9841 ccgcatatcc agtggtaggc acgcgctttg tcgttgaaga aaaagtgtcc gacaaacacc
     9901 atcagcagtg gctcgagtcc gacaatgacc gatgcgctgg cggcggaagt gtatttcaac
     9961 ccgacaaact gaagcagcag ggtcagcaca tagttgacga acgacacaat cagcaacggc
    10021 ttccattcct cacgcggaat cttgccgaca tgacggcggc aggcgggcag tgcaggcagc
    10081 gcagcaatca gcaggcgcac gccgaccatc aatgcgggat cgatgccgcc atagacatat
    10141 ttggcggcaa taaacgagct gctccagata atcagggcaa ggatttggta aaacatagtg
    10201 agtgataaag tcagatggtc atacaaaagt atagcggatt aacaaaaatc aggacaaggc
    10261 gacgaagctg cagacagtac aaat
//
LOCUS       DAWFBO010000067        10273 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFBO010000067 DAWFBO010000000
VERSION     DAWFBO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.067"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(76..1497)
                     /locus_tag="WGO87_001171"
     CDS             complement(76..1497)
                     /locus_tag="WGO87_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0392533.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1857..2216)
                     /locus_tag="WGO87_001172"
     CDS             complement(1857..2216)
                     /locus_tag="WGO87_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0392534.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFYRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2213..2818)
                     /locus_tag="WGO87_001173"
     CDS             complement(2213..2818)
                     /locus_tag="WGO87_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0392535.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2865..3347)
                     /locus_tag="WGO87_001174"
     CDS             complement(2865..3347)
                     /locus_tag="WGO87_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0392536.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3551..3648
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3859..4959
                     /gene="gcvT"
                     /locus_tag="WGO87_001175"
     CDS             3859..4959
                     /gene="gcvT"
                     /locus_tag="WGO87_001175"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0392537.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5121..5507
                     /gene="gcvH"
                     /locus_tag="WGO87_001176"
     CDS             5121..5507
                     /gene="gcvH"
                     /locus_tag="WGO87_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0392538.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANVADYDGLLTAEQYAGEVD"
     gene            5652..6899
                     /locus_tag="WGO87_001177"
     CDS             5652..6899
                     /locus_tag="WGO87_001177"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0392539.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFSAAVLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     AAASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7175..7600
                     /locus_tag="WGO87_001178"
                     /pseudo
     CDS             7175..7600
                     /locus_tag="WGO87_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            7606..8835
                     /locus_tag="WGO87_001179"
                     /pseudo
     CDS             7606..8835
                     /locus_tag="WGO87_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            8893..9513
                     /locus_tag="WGO87_001180"
     CDS             8893..9513
                     /locus_tag="WGO87_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0392540.1"
                     /translation="MTTHHVELRNVTKRFGAQKAVNQVDLVLKAGESVGLAGYNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            9602..9733
                     /locus_tag="WGO87_001181"
                     /pseudo
     CDS             9602..9733
                     /locus_tag="WGO87_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9730..10224
                     /locus_tag="WGO87_001182"
     CDS             9730..10224
                     /locus_tag="WGO87_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0392541.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVSFDDMPDT
                     YIFK"
BASE COUNT         2624 a         2527 c         2815 g         2307 t
ORIGIN      
        1 tgtcggaaag gaaaaatatt gatttgatta tgccggaatt taaaatgccg tctgaatgtt
       61 cagacggcat agcgtttaca gcagtttgaa aacgaaaaag ataagggtat gtacgatgaa
      121 gacgggtgtc aggaaggcga ccgaccacat catatagccg aagaaagtcg gcatcggtac
      181 gccgcgctgt tcggcaatgg ccttgaccat gaagttcggt gcgttgccga tgtaggtcag
      241 tgcgcccatg aataccgaac ccatagaaac cgccagcagc gaatgaaaca gggtacccgt
      301 catcaaggct tgggcatcgc cgcccgccat attgaaaaaa acgagataag tgggcgcgtt
      361 atccaagaat gccgacaata tgccgctcat ccaaaaatac atcacattaa tcggatgacc
      421 tgccgtatcg tgaaccagcg ataccacccc gcccagcgcg cctgcctcgc ctgctttcag
      481 aatgctcagg acgggaaaga tggtgatgaa gatgccgagg aagagtttgc ccacttcggc
      541 gatgggttca aagttgaatt cgttgcctgc gcggacttgt ttgggcgtga ttgccataga
      601 tacggcggtc aatgcaatca ggatgacatc gcggacgagg ttttgcaggg cgtaacggct
      661 gccgaggatt tcaaatcccg ggtgttcggg tttccaaagg ccggacatta gaaccgcgcc
      721 gaccacgccc gaaagcagga ggaagttcca tttgccgaag atggcgattt tttcgggttt
      781 ttcctgttgt gccggcgtat cttgtgcaat gctttcctgt ttgaagaaac ggttgtcgat
      841 gaaatagaag gcggtcaaca ggacagcggt gctgatcagg acgggggcga acatatgttt
      901 gaccgtccac atgaaatcta cgcctttgag gaagccgagg aagagtgggg ggtcgcccaa
      961 aggggtcaga ccgccgccga tgtttgcaac caggaaaatg aagaagatga cgatgtgcac
     1021 gcggcgggta cggttttggt tggctttcag cagcggacga atcatcagca ttgctgcgcc
     1081 ggtcgttccc atgatagagg caagtgccgt accgacggca agcagggcgg tgttgagctt
     1141 gggtgtgccg ttcaagtcgc cccaaaccaa aatgccgcct gaaatggtgt acagggcaag
     1201 cagcagcagg atgaaaggga tgtattcttc aacgagtgcg tgtgcgacgg tatggatacc
     1261 ggcggacgcg ccaaaaacca aactgaacgg gatgaggaag agcaatgtcc aaaaggcggt
     1321 aattttgccg taatggtgat gccaggtatg cgaaaaaaac aagggaccca atgcgataga
     1381 cagcaaaatc agggcaaagg gcaggcccca cagcaggttt aggtttgcgc cgtccaaatc
     1441 tgcggcgtaa accgatgctg ggaaaagcat tagtgaaaac aggggtaggt ggcgcatcgt
     1501 gtttcctcga ttcaagcact gccttgcgcg gcgcgtggga gtgatacagg caccgtgccg
     1561 cccggacata ggcggactgt gctgcctgtt tgtttttgaa aaaagttttc cgattgattg
     1621 taaagtaaat aatcaggata aaccagtttc ccaaaccgga aggcggcggg aaggcggatt
     1681 gctgtgcttg ggaatatatg ttctttttga taaataattt tatttaaaac aaataaatat
     1741 atgtctttaa taaataatct atcgaaaacg aaaaatgaat ttattttaac atatatttgc
     1801 aatgaaacag gtttgccccc cccgtttgtt tgcccttatc cctttcagta cggcattcaa
     1861 gattcgggcc tgcgccacat ccatatggcg acaagggaac aaaaaaccga tgaaaccgcc
     1921 ccgacccacc agcgttgggg aaactgccaa aacattatca ggcaggatgc ggtcatcata
     1981 ctgatggcga atattttggc tttgcgcggc actgcgccgt tttgttccca gttatgaacc
     2041 atcgggccga aatagcggtg ccggtgcagc cagcggtaaa agcgcgggga tgcctttgcc
     2101 cagcaggcgg cggagagcag tacgaacggc gtggtcggca acagcggcaa aaaaatgccg
     2161 atgataccca acagcaggga gatgcagccg caggcaatta aaagataacg tatcattttg
     2221 aaatattttt cttattgtgc ggataagggc aggatgtgat accgagtttt gcccagcctt
     2281 catgtcccat tttttccagc agggcgatat tgcgttcgaa tattgccgaa gcgtcgggaa
     2341 aggcttgtgc ggctttggca atgctgtctt cgcggatgag gtgcagcgtc ggatagggag
     2401 aacggttggt gtagttgccg atgtcgtcgg gttcggtatt ctgaaagcgg aattggggat
     2461 ggaacggtgc gatttggacg atgccttcca agccgttttc gataacggcg gcatcggcaa
     2521 tatcgagcat atcgttgaat acgtcgaaat cggggaatag ggtcgggtga accagcaggg
     2581 tggtttccag ttcggtggcg ggtgtattgc ccagtcgctg cagttcttcg tccaagtctt
     2641 ccaaaaaact gtcaaggtgt ttggcttcgc tgattgcgat gcggacaagg tttttaacgt
     2701 ggggggcttt ggcaaaggga cacaggttca gaccgatgac ggctttttcc aaccattgtc
     2761 cggtgtgttc ggcaacagca tctttatttt cggaagtatt gatattcatt attgtcatgt
     2821 aaatgtgttt gcagattgca cgtgcgggaa aatcgggaag ggcactattc cttcagcagg
     2881 tggttgagcg gcagggaggt ggtgtgtttg atttctttta aaacaaagct cgattgcgca
     2941 tcttgtacgc cgtggtggga caggagcgta tccaaaacaa aatgggaaaa cgcgttcata
     3001 tcggtaaaaa acgcctgaag caggtagtcg gtttcccctg tcagggcgaa gcagctcaag
     3061 acttcaggcc attttcgaac cgatgcggca aagtcttccc gcgcgtcttt tgctttgcgg
     3121 atggaaacgc ggataaatgc ctgaagcccc aggttgacgg attccggaga cagcagcgcg
     3181 gcatattggc ggacgatgcc ggcatcttcc aactgcttca gacggcgcag gcacggagaa
     3241 ggcgaaagtg cgacacgttc ggacagttcg acattggtca gcctgccgtt ttcctggaga
     3301 acctgtaaga ttttaatatc ggttttgtct aaagtgagtt ggggcatatt tgcgttccgt
     3361 tttaaggaat tcggattgtc tgtccgtatg tttgcggcaa tccgcacaga tggagaccat
     3421 attaacatat aaaaagttat accgtcatcc gggacaaatt ttgttttcgg aaaatcatgt
     3481 gaaaacagag gcggtcggtt tgcatctctt ttaagacggc ttgcccaaac cgccgattca
     3541 agacataatc gggaaatgtg caggagagtg ttacacccaa ctacaatgta accaccgaag
     3601 gcgcagacac ccttaaatcg ctcaggtatc agggactgca cattgaaaca aacaatctgg
     3661 agagcggcgt tggaataacg tccaccgaag gggagaaggc cgtctgaacc accattcaga
     3721 caaccgcgca aagcagtgag cagactggtt tgccatcatg cggatacggc cgaaaatctc
     3781 aggttcaagg acagataggg tcatccgcgc acaggtgcgc gggtggcatc tgaacaaaaa
     3841 atccggagaa acttgagaat gattactctg aaaaccaccc catttcatca agcccatcaa
     3901 gatgcaggcg cgaagccggt agattttgcc ggctgggagc tgcccatcca ttatggttcg
     3961 caaatcgccg aacacgaagc cgtgcgcacc gacgccggta tgtttgacgt atcccatatg
     4021 cttgttaccg acgtagcagg cgcaaatgcc aaagcctttt tccgcaaatt gattgccaac
     4081 gatgtcgcca agctcgcttt tgtcggcaaa gccctttatt ccgctttgct caacgacaac
     4141 ggcggcgtga ttgacgactt aatcgtttac cgcaccaacg aagccgaaac ccaataccgc
     4201 atcgtgtcca acggagcgac tcgcgaaaaa gacacggcgc aattccacaa agtcggacaa
     4261 gagttcggcg ttgcctttaa tccgcgctac gacctcggca tgctcgccgt acaaggccct
     4321 aaagccatcg aaaaactcct gaccgtcaaa cctgaatggg cggatgtgat tcacggcctc
     4381 aaaccgttcc aaggtgcaga cttgggcaac gattggtttg tcgcccgcac cggctatacc
     4441 ggcgaagacg gcgtcgaagt gattctgccg ggcactgaag ccgtcgcatt cttcaaagcc
     4501 ctgcaagcag caggcgtaca accttgcggt ctcggcgcgc gcgacaccct gcgcatggaa
     4561 gccggcatga acctctacgg caacgatatg gacgacgata ccagcccgct cgaagcaggc
     4621 atgggttgga ccgttgattt gaaagacgaa agccgcgatt ttgtcggcaa agctgccttg
     4681 ttggcattga aagaaaaagg cgttgccgtc aaacaggtcg gtttgctgct cggcaaaggc
     4741 ggcatcctgc gcgcgcatat ggaagtgttg accgacaaag gcaaaggcga aaccaccagc
     4801 ggcgtattct cgcccagcct gaaacaatcc atcgccatcg cccgcgtacc gaaagacttt
     4861 gacggcgata ccgctaaagt gctgatgcgt ggcaaggaag tggacgtgcg cgtattgaag
     4921 ttgccgttcg tccgcaacgg tcaaaaacag tttgattaat atcggttcag acggcatttt
     4981 catttcatat gccgtctgaa agcaggtttt aattgttgtc cgatacggac gtttgtagaa
     5041 agccttgaac aaggtatctg tggatattga ttcatataga tgccgtctga aaataacccc
     5101 taccaatgga gaatcaaacc atgagcaaca acatcccagc agaactgaaa tatgttgcca
     5161 gccatgaatg gctgcgcctt gaagaagacg gtaccatcac cgtcggcatt acccaccacg
     5221 cgcaagagct gttgggcgac atcgtgttcg tcgagctgcc cgaagtcggc gcaaacctgg
     5281 ctgccgaaga gcaagccggt gtggttgagt ctgtaaaagc cgcatctgac gtgtacgcgc
     5341 cgattgcggg cgaagtcgtt gccgtcaacg atgacttgcc cggcgcacct gaaactgcca
     5401 acagcgaccc ttacggcgca ggctggttct tcaaaatcaa accggcaaac gttgccgact
     5461 acgacggtct gctgactgcc gaacaatacg cgggcgaagt ggactaaacc ctatcctgat
     5521 aatgggatgc ctgatttgag aaactgcact ttcagacggc aaggatgcgt ttggaagtgc
     5581 ggtttttgtc tgtgtcttca aggtcagtta ggctataata acggtctata tccatcttta
     5641 ccgatttttt catgcaactt accgctgtcg gactcaatca tcaaaccgca cctttaagca
     5701 tacgggaaaa gctggcgttt tccgccgccg tcctgcccga agccgtccgc aatcttgccc
     5761 ggagcaatgc ggcaacggag gcggtaatcc tttctacctg caaccgcacc gagctttact
     5821 gtgtcggcga ttcggaagaa atcatccgat ggcttgccga ttaccacagt ttgccgattg
     5881 aagaaatccg tccgtatctg tacacgctgg atatgcagga aaccgtgcgc cacgccttcc
     5941 gcgtcgcctg cgggctggat tcgatggtgt tgggcgagcc gcagatttta ggacagatta
     6001 aggatgccgt cagggttgct caagagcagg aaagtatggg taagaaactc aatgccctgt
     6061 tccaaaaaac cttttccgtt gctaaagagg tccgtaccga tactgccgtc ggcgagaact
     6121 cggtttctat ggcggccgct tccgtcaagt tggcggagca gatttttccc gacatcggcg
     6181 atttgaatgt cttgtttatc ggcgcaggcg aaatgattga gctggttgcc acttattttg
     6241 ccgccaaaaa tccccggctg atgacggttg ccaaccggac gctggcgcgt gcacaggagt
     6301 tgtgcgacaa gctcggtgtc aacgccgaac cgtgcctgct gtccgatctg cctgccattt
     6361 tgcatgagta cgacgtggtg gtttcttcaa cggcaagcca gttgcccatt gtcggcaaag
     6421 gtatggtgga gcgcgcattg aaacaaaggc agagtatgcc gttgtttatg cttgacttgg
     6481 ccgtgccgcg agacattgag gcggaagtcg gagatttgaa cgatgcctat ctttatacgg
     6541 tggacgatat ggtcaatatc gtccaaagcg gcaaggaggc aaggcagaag gccgccgccg
     6601 ccgccgaaac gctggtgtcc gagaaggttg ccgaatttgt caggcagcag cagggcaggc
     6661 agagtgtccc gttaatcagg gcattgaggg atgagggaga gaaagcgcgc aaacaggtct
     6721 tggaaaatgc gatgaaacag cttgctaaag gcgcaacggc agaagaggtt ttggaacggc
     6781 tgtccgtcca actgaccaac aagctgctgc attcgccgac ccaaactttg aataaggcgg
     6841 gggaagaaga taaggatttg gttcatgccg tcgcgcagat ttatcatttg gacaaataac
     6901 ggtgcgccgg gaaaaatgcc gtctgaagag gtttcagacg gcattttttt gtgccgcctg
     6961 acaacatcgt aaaatcccac attatatcga tgtaatcaca aagtatagtg gattaacaaa
     7021 aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg
     7081 ctgtactggt ttaaatttaa tccactatat tatcccgtat gcggattggt tttaagattt
     7141 gtaaatttaa tttgcatcaa aaaatcgccg atagatgatt catataatat caatattaaa
     7201 gagtatcggt atatcgggga tagtcatgtc ctgtttttca atcaaacgta tgtccgcgtt
     7261 tcgggcgcgg ataacggcgt tttttgccgc ctttgtcttt ttgacggcgg cactgcccgc
     7321 ttatgcggag catctgcctg attttctggc gaaaatacag ccttcggaaa tttttccggg
     7381 tgcggaccgt tacggcaagc cggaaggtaa gcctatggtt gcccgcgttt acaaaggcga
     7441 tgagcagttg ggcttggtct atatcacgac cgatgcggtc aatacgcgcg gttattcgag
     7501 caaaccgatt gatacgctga tggcgttggc aaacgacgga acgatagccg gagcgaaatt
     7561 gattgatcac catgagtcga ttatgctgat cggtatcccg catttgcccg cgcccgggcg
     7621 ggcgatacgc tcaaactggc ttccggcgta tatcaaacca aactttatat cgacaaaccg
     7681 attacgattg aaggacctgc cgaccgttcc gcaaccatcg aaggggacag gagcggtcgt
     7741 acgatagctg tgcatgcgcc ggacgtaacg ctccgcaacc tgaccgttac acgttccggt
     7801 atgagcctgc cagcaatgga tgccggtatt tatctcgaag aaaccgcccc acgcgccctg
     7861 attgaacaca acaatatttt tgataattcg gtcggcgtat atctgcatgg ttctgccgaa
     7921 gcgatggtgc gcgagaataa aatcgtcggc gacgcgactt tgcgcgtgaa cgagcgcggc
     7981 aacggcgtta ccgtttggaa cgcacccggt gcgcaggtcg tcggcaacga catttccaaa
     8041 gggcgggacg gcattttttc caacaccagc acgcacaaca cctataaaaa caaccgcttc
     8101 agcgatttgc gtttcgccgt ccactatatg tacaccaacg acagcgaagt cagcggcaat
     8161 atttccgtgg gcaacaatat gggctatgtg ctgatgtttt ccgaacggct caaagtattc
     8221 gacaatattg ccgtcggtag tcgcgactag ggcatcatgc tcaactatgt caactattcc
     8281 gatattcacg acaatattat caacaaagcg ggcaagtgcg tttttgccta caatgccaac
     8341 tacgataaac tgtccgccaa tcattttgaa aactgccaaa tcggcataca ctttaccgcc
     8401 gccatcgaag gcacgtctct gcacgacaat tcctttatca acaacgaaag ccaggtcaaa
     8461 tacgtcagca cgcgctttct cgactggagc gagggcggac acggcaacta ttggagcgac
     8521 aacagtgcgt tcgatttgaa cggcgacggc ttcggcgaca gcgcgtaccg gcccaacggc
     8581 atcatcgacc aaatcatctg gcgcgcgccc gtattgcgcc tcttgatgaa cagtctcgca
     8641 atcagcatcg tcaaatgggc gcaggcgcag tttcccgccg ttctgcccgg cggcgtggtg
     8701 gacagcaaac cgctgatgaa gccttatgtc cccaaaattc aaacccgcta tcaggcaatg
     8761 aaggacgagt tgctcaaaga agccgaaaca cggcagtcgg aatggggcag ggcggaaaac
     8821 ggttctttga actagtctgc ttcagacggc atccggatcc aaatgccgtc tgaaaacaca
     8881 aaaggaacaa ccatgaccac acatcatgtc gaattgagga atgtcaccaa acggttcggg
     8941 gcgcaaaaag ccgtcaacca agtcgatttg gttttgaagg caggagaaag cgtcgggctt
     9001 gccggataca acggcgcggg caagtccacc attatgaagc tgatactcgg gctgattacc
     9061 ccgaccgaag gcgaagtgat gcttttgggc gaacgtaccg gcagcaaagc gggggcgcgg
     9121 cttcgcagcc aaatcggcta cctgcccgaa actgttgcgt tgcacccttc gctgaccggc
     9181 atcgaaacgc tggattttta tgccaaactt aaaaaacagc cgctcacgca gaatcggggg
     9241 ctgctcgagc gcgtcggcat ttcacaggcg gcacaccgcc gcgtcggcac ttattctaaa
     9301 gggatgcgcc aacgccttgc cttggcacaa gccctgctgg gcgagcccaa agtcctgctg
     9361 tttgacgaac cgacaaccgg cctcgaccct gcatcaaggc aaatgtttta cgaagtcgtg
     9421 cgcgaactca acgggcgcgg cgcgaccgta ttgctcagta cccacgccct tgccgagttg
     9481 gacggacacg ccgaccgcat tatcgtggat taaatttaaa ccagtacggc gttacctcgc
     9541 cttaccgtac tatttgtact gtctgcggct tcgttgcctt gtcctgattt ttgttaatcc
     9601 actatatgcg ggtatggcgg gtttgagcgg acaaatcagc ctgaccgtcc ccgttttgct
     9661 gaccgctcag gttttatggg ttatcattcc gcttgttttg gcagccggaa tttttagaaa
     9721 gcgacaaata tgaaaaaaac cctgttggca attgttgccg tttccgcctt aagtgcctgc
     9781 cggcaggcgg aagaggggcc gccgccttta ccccggcaga ttagcgaccg ttcggtcgga
     9841 cactattgca gtatgaacct gaccgaacac aacggcccca aagcccagat tttcttgaac
     9901 ggcaaacctg accagccagt ttggttctcc accatcaagc agatgttcgg ctataccaag
     9961 ctgcccgaag aacccaaagg tatacgcgtg atttacgtta ccgatatggg taatgttacc
    10021 gattggacaa atcccaatgc cgacacgaag tggatggatg cgaaaaaagc cttttatgtc
    10081 attgacagcg gttttatcgg cggtatgggt gcggaagacg cactgccgtt cggcaacaaa
    10141 gagcaggctg agaaatttgc aaaggataaa ggcggtaagg ttgtcagttt cgacgatatg
    10201 cctgatacct atattttcaa ataaatccaa acaaaaatgc cgtctgaacg ttgaagccaa
    10261 ggttcagacg gca
//
LOCUS       DAWFBO010000068        10055 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFBO010000068 DAWFBO010000000
VERSION     DAWFBO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.068"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            15..689
                     /locus_tag="WGO87_001183"
     CDS             15..689
                     /locus_tag="WGO87_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0392542.1"
                     /translation="MQADLAYAAERITHDYPQATGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFGDWRIAADYASYRKWKESNFSTKKVTKEINDNYQETKTEHQGNGSFHASSSL
                     GLSAIYDFKLNDKFKPYIGARVAYGHVKHQVHSVETKTTTVTPKPKNGSPQGGAVPSR
                     EPSKPPYHESHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSL
                     GVRYRF"
     gene            complement(663..872)
                     /locus_tag="WGO87_001184"
     CDS             complement(663..872)
                     /locus_tag="WGO87_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392543.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKCA
                     GKGLMPSEGSDGIGIGESEAVAHAQ"
     gene            complement(890..1447)
                     /locus_tag="WGO87_001185"
     CDS             complement(890..1447)
                     /locus_tag="WGO87_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0392544.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1523..3157)
                     /locus_tag="WGO87_001186"
     CDS             complement(1523..3157)
                     /locus_tag="WGO87_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0392545.1"
                     /translation="MIKPNLRPKLGSSVLIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGDIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3562..4668)
                     /gene="serC"
                     /locus_tag="WGO87_001187"
     CDS             complement(3562..4668)
                     /gene="serC"
                     /locus_tag="WGO87_001187"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0392546.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            5033..5464
                     /gene="rimP"
                     /locus_tag="WGO87_001188"
     CDS             5033..5464
                     /gene="rimP"
                     /locus_tag="WGO87_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0392547.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5493..6995
                     /gene="nusA"
                     /locus_tag="WGO87_001189"
     CDS             5493..6995
                     /gene="nusA"
                     /locus_tag="WGO87_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0392548.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            7008..9896
                     /gene="infB"
                     /locus_tag="WGO87_001190"
     CDS             7008..9896
                     /gene="infB"
                     /locus_tag="WGO87_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0392549.1"
                     /translation="MNNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAATRAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAGKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRVGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKT
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKEAANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELAAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQLTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGE
                     LSSLKRYKDDVKEVRMGFECGLMIKGFNEIMEGDQLECFDIVEVARTL"
BASE COUNT         2693 a         2900 c         2539 g         1923 t
ORIGIN      
        1 gcagcccgta ttatgtgcag gcggatttag cctacgccgc cgaacgcatt acccacgatt
       61 atccgcaagc aaccggtgca aacaacacaa gcacagtaag cgattatttc agaaacatcc
      121 gtgcgcattc catccacccc cgggtgtcgg tcggctacga cttcggcgac tggaggatag
      181 cggcagatta tgccagttac agaaaatgga aagaaagtaa tttttctact aaaaaagtta
      241 ctaaagagat aaacgacaac taccaagaaa ccaaaacaga acatcaagga aacggcagct
      301 tccacgcctc ttcttctctc ggcttatccg ccatttacga tttcaaactc aacgataaat
      361 tcaaacccta tatcggtgcg cgcgtcgcct acggacacgt taaacatcag gttcattcgg
      421 tggaaacaaa aaccacgact gttaccccta aaccaaagaa cggctctcca cagggaggag
      481 ctgtcccatc aagagagccc agcaaacctc cctatcacga aagccacagc atcagcagct
      541 tgggtcttgg tgtcatcgcc ggtgtcggtt tcgacatcac gcccaagctg accttggaca
      601 ccggataccg ctaccacaac tggggacgct tggaaaacac ccgcttcaaa acccacgaag
      661 tctcattggg cgtgcgctac cgcttctgat tccccgatac cgatgccgtc tgaaccttca
      721 gacggcatga gacctttgcc tgcgcacttg gtgcgctggt cgcctccgaa catggcgcga
      781 cacccgacat ttccgccgaa cgcatcgggc gtttcatgaa tccggtttaa aacgcatgga
      841 aaaatgccgt ctgaaagcct ttcagacggc attgtgcttg agattccgtt taccaatgac
      901 tgacaaacgc ttccaaatcg gtattcttgg gcttatgcac ttcctctgtc ggcgtgccga
      961 ccatcatcag cccgatgatt ttatccttat ccgcacatcc gaaagcctca cgcaacaggg
     1021 ggctgttgac ccacatcccc gtaatccaga cattgtcgaa accttgcgcc gttgccgcca
     1081 gttgcagcgc atacgccgca caacccgccg tcagcatctg ctcccattcc ggtttcggct
     1141 taggcacatc gcggttcggc gcaaacgtta ccccgataac catcggcgcc atattgccca
     1201 ctttttccgc ctttttcatc gcatcgtcgc cgaaattcaa ttcggcaacc gtttgcttca
     1261 acacatcgcg aaaacgttgc aatcctacct cgccttgaat cacggtaaaa cggaaggggc
     1321 gcatattgcc gtgatcggga acttgggttg ccgcctgaaa tatttgttcc aactccgccg
     1381 catcgggggc ggggtgcttc agctttttgg aagatcggcg gttcgtcaat aattttaaag
     1441 catccatatc gttatactcc ggtcatcggt cgggtgttcg gacacatatg ccgtccgaag
     1501 gcttcagacg gcatatccgg catcagcgcg gacggcggca ggctgccaat atatccattt
     1561 ccttccgata ggtttggctg ttggaaatgt ccatcagccc caataccgtg ctgaaatagt
     1621 ggtcgtgcga atattcgttt tccgccgctt tttgtttgag gcattggaaa tctatgtccc
     1681 cgtgttggcg gaaggctttg gaaaaccaca taaccatcgg gatatgcgtc tgcccagaag
     1741 gcgcgatggc gtaaggcgcg gcgtgcaggt acatcccgtt ttcgcccaaa ctttcgccgt
     1801 ggtcggaaac ataatgcacc acgctttcca aatcgtcgcg gttttcaagt ttgcggataa
     1861 ccttgtcgat aaactggtcc acatacaaaa ccgtattgtc gtaagtgttg accagcgtgg
     1921 cgcgggtgca tttgttgatt tcgttggtgt cgcaggtcgg cgtgaatttg cgttcggctt
     1981 cggtatagcg ttcgtaatac gtcggcccgt ggctgccgat ggtatgcagg attaaaaccg
     2041 cgtctttatc gtttttgttg aggacttcgt cgaacttagt cagcaggata ttgtcgagac
     2101 actcgccgtt gcggcagtat tcgggcaggt tgagcgaggt aacgtcggta ttcggcactt
     2161 tgccgcacac gcccttgcag ccggaatcgt tttccaacca ggtaacttcc acgccggcgc
     2221 gctgcacgat gtccagcagg ttgtcttggt gttcggcttt gatttcgtca taatccgtgc
     2281 ggtcgaaggt tgagaacata cacggcaggg agtgcgcggt cgatgtgccg cagcttctga
     2341 cctgcgggaa attgacaatt tcatcgccgc gcgcggcaag cagcggcgta gtttggcggc
     2401 tgtaaccgtt caaaccccag ttggcggcac gcgtggtctc gcccacgacc aaaaccacga
     2461 aacggcgcag gctgccggcc ggccggtttt gcacgaccgc catatccaat tgcgtataag
     2521 gaatattgga acgcttccaa tctttgtatt tcgacacgcc cgcgccgatg aaattagacg
     2581 gcacaatcag atgggttact gatttattgt tgcggaaaaa cgaggcgtaa tcctgatatt
     2641 gcaacattgc gatgcccaac gcgcacaaaa aggaaacggc ggcaagcaca aggcgcgtca
     2701 aaagctcctt ataccaaacg cggtatttaa ccttgacggc gatatacgcc agcgcgggca
     2761 atacgcccaa acatacaatc cacagcacat agcccggcgt aatcaggcgc gcgctttcgg
     2821 cagccgtagt ttgcaagaca ttattcaaca tcgacttgtt gaaatagata ttgaaaaata
     2881 tttcttggta agacaccgcc gcactgataa ccaatatcaa tggaatcaat accttatgca
     2941 cgaaaggcag ggcaatgacg tgaaaaacga aattacttaa aaaaaacagc accaccggca
     3001 tcgtatagag gaagatatcc gccccggtgc cgttaaaagg atgaagctcg acaactttgg
     3061 caaaaaaggc gtaattcaat accagcgagg aatacaggga aaggaaggca atcagcacgg
     3121 aagagccgag cttcggcctc aggttcggtt ttatcatttg gaatgtgtcg gataagggtt
     3181 ggaaaaggca tccggcattt ggaatccgga ttattgaaaa agattcttaa ttataaggca
     3241 acggagcaaa gcagggcaag aaaacggcgg cggcgcgagg tttccgcccg ccattcaaac
     3301 gtccggcaga cataaaaaca tcgtaagcaa gattcgaacc ggtctgcaac cgcccctgcc
     3361 agaaaaacgg gcaaagcatt tcatattgga aaaacccggc cgcgccgccg acgggacagt
     3421 ccggcacaaa cagcatcacg cccggattga aaaggacgga tagccgccgg cagcacagtt
     3481 ttaccccgca tcccaaaacg ccgccggaga acgccgaaac agccgatgcc gtctgaagcc
     3541 gcttcagaca acatcgggac atcaaccgta acgccgttgg aaatcgcgca taaaatctgc
     3601 caaagcccgc acgccttcga gcggcatcgc attgtagatg ctggcgcgca tcccgccgac
     3661 agacttatag cccttcaaca ggcacaagcc ctgcaattcg gcttccagca caaaacggcg
     3721 gtcaagctcc tcatcccccg tttggaacac gacattcatt ttagaacgcg cattcggacg
     3781 gatacggttg atataaaaac cgccactgcc gtctatcgtc tcatacaagg tttgcgcctt
     3841 cagccgattg accgcttcaa tttttttcac accgccctgc gcctgcagcc agcggaacac
     3901 cagccccgac atataaatcg cgtaagttga cggcgtgttg tacataccgt cgcggttgat
     3961 gtgcgaacgg tagttgaaca catcgggaat atcgttcgga caacgctcga gcaaatcctc
     4021 acgcacaatc accaccgtaa ctcctgccgg cccgatgttt ttctgtgcgc ctgcgtaaat
     4081 cagtccgtag tcggcaacat caaactcgcg cgacaaaatc tcgctggaca tatcgcacac
     4141 cagcggcggc atgccttctg aaaggcgcgg cacttcacgg tattgcagcc cgttgaccgt
     4201 ttcattgacg gcaaaatgga caaacgccga atcgggtgca acatcccacg tttccacagg
     4261 cggcaggtcg agatagtcga actgctcgcc gccatgcgcc gccaaacgga tttccgtatc
     4321 ggtcaaacgg ctcatctgtt cataagcgat acggctccag ttgcccgtta ccaccgcgtc
     4381 ggcagtgcgg aaaccgtgtg ccagattcat ggctgccata ttaaattggg ttgttgctcc
     4441 gccctgcaga aacaatatct tatagttgtc aggcactttc aaaagctgcc tcaaatcctg
     4501 ttccgcatga tgcaggatgc tcaaaaacat ttccgaacgg tggctcatcg ccatcacagg
     4561 aaaacccgta ccgttgtagt ccaacatttc ctgccgcgcc gtttccaaca cggcttcggg
     4621 caatacggca gggccggcgg aaaaattgta aatcggataa agagacatga tgcagccctg
     4681 attctgaaca ataacccgcc tgattttagg cttgcagcag gctttgtgca aggatgggaa
     4741 atacctgtcc tccgcccggc ttcatgccgc ccgaacacgg aaaataatat caatatattg
     4801 atttacaaac ataaaaatca tgtacgcgac aaatagatac atttgttttg tcaacaatat
     4861 tcacgatttc ccattacaaa cctcccttac acccgctttt ttccgtccca aaaacacaaa
     4921 ataaatcaac actttcattt ctccgcaaaa gcggttataa tcacgccgat ttttcaactt
     4981 tgacgaaaaa tggccacggg gccatttttt tattatgtac atagggagca gcatggatat
     5041 ccaaaccatc ctcgaaaaaa ccctgcccgg cctgggctac gaactggtcg attttgaact
     5101 gaccgcgcaa ggaacattgc gcgtgttcat cgacaaagaa ggcggcatta ccgtcgaaga
     5161 ctgcgcaacc gtcagcaacc acttgagccg cgtcttcatg gttgaagaca tcgactacaa
     5221 aaacctggaa atttccagcc ccggactcga ccgccccttg aaaaaagccg ccgacttcgt
     5281 gcgctttgcc ggtcagaatg ccaaaatcaa aacccgcctg ccgatagacg gtcagaaaaa
     5341 ctttatcggt aaaatcgaag gctgcgaaaa cgataccgtt accgtatcct tcgacggcaa
     5401 aaccgtacaa atcgaattgg gcaacatcga caaagcccgt ctgcgccccg aattcaaatt
     5461 ctaaaacaca acaataattg gagatgttca aaatgagtcg tgaaatgtta cagctggcag
     5521 aagcactggc aagcgaaaaa aacgttgatg ccgaagtcgt cttccaagca ctggaattcg
     5581 ccctgtctac cgccgccaag aaaaaggcag accgcgaaca catggacgtg cgcgtccaaa
     5641 tcaaccgcga taccggcgaa taccaaacct tccgccgctg gctgattgtc gccgatgaag
     5701 actataccta tcccgatgtc gaaaaaacca tcgaggaaat ccaagaggaa attcccggca
     5761 ccgccatcca aatcggcgaa tactatgaag agcagctgcc taacgaaggc ttcggccgcc
     5821 aagccgcaca aaccgccaaa caaatcatcc tgcaacgcat ccgcgatgcg gagcgcgagc
     5881 aaaatctgaa cgagtttctc gccgtcaaag aagacatcgt gtccggtacg gtcaaacgcg
     5941 tcgaacgcca cggcatcatc gtcgaagtcg ttgccggcaa actggacgcg ctgattccgc
     6001 gcgaccaaat gattccgcgc gaaaacttcc gcagcggcga ccgcatccgc gccctcttcc
     6061 tgcgcgtcga agaaatcggc aacaccggcc gcaaacaagt catcctgagc cgtacttccg
     6121 gcgatttcct cgtcaaactg tacgccaatg aagtacctga aattgcagac ggcatgcttg
     6181 aaatccgcgc tgtcgcccgc gacccgggcc agcgtgccaa agtcgccgtc aaagccaacg
     6241 accagcgcat cgatccgcaa ggcacctgta tcggcgttcg cggttcgcgt gtcaatgccg
     6301 tcagcaacga attgtccggc gagcgcatcg acgttgtcct ttggtcgccc gagcctgccc
     6361 aattcgtcat gagcgcgctc tcacccgccg aagtcagccg catcgtcatc gacgaagaca
     6421 aacacgccgt cgatgtcatc gttgccgaag accagctcgc gctcgccatc gggcgcggcg
     6481 gtcaaaacgt gcgccttgct tccgacctga ctggctggca gctcaacatc atgacttccg
     6541 ccgaggcaga cgaacgcaat gcggcagaag atgccgccat ccgccgcctg tttatggatc
     6601 acttgaacgt ggacgaagaa accgccgacg tactggttca ggaaggtttt gcaaccttgg
     6661 aagaagtcgc ctatgttcct gccgccgaac tgcttgccat tgaaggattt gacgaagaaa
     6721 tcgtcgatat gctccgcaac cgcgcccgcg atgccatcct gaccatggcg attgccgccg
     6781 aagaaaaact gggcgaagtg tccgacgata tgcgcaacct cgaaggcata gatgccgata
     6841 tgctccgcag ccttgccgaa gcaggcatta ctacccgcga cgacttggca gagcttgctg
     6901 tggacgaact gattgaaatc accggtgtaa acgaagaaac cgcaaaagcc gtcatcctga
     6961 ccgcacgcga acactggttt accgaagaca aataaaaggg ggtacagatg aataacacaa
     7021 ccgtagaaca atttgccgcc gaactgaaac gccccgtcga agacctgttg aaacagttga
     7081 aagaagccgg cgtcagcaaa aacagcggca gcgattccct gacgctggac gacaaacagc
     7141 ttctgaacgc ctacctgacc aagaaaaacg gcagcaacgg cggcaccatc agcatccgcc
     7201 gcaccaaaac cgaagtcagc accgttgacg gcgtaaaagt cgaaacacgc aaacgcggac
     7261 gcactgtcaa cattccttct gccgaagaat tggcagcaca ggtaaaagcc gcccaaaccc
     7321 aagccgcacc tgtccggccg gagcagacgg cagaagacgc ggcaaaagcc cgagccgaag
     7381 ctgccacacg tgcagaagcc cgtgccaagg cagaagcgga agcggcaaaa ctgaaagcgg
     7441 caaaagcagg caacaaagcc aaacctgccg cgcagaaacc caccgaagca aaagccgaaa
     7501 ccgcacccgt tgcggcggaa accaaacccg ccgaagaaag caaagcggga aaagcccaag
     7561 ccgacaaaat gccgtctaaa aaacccgccg agcccaaaga aaaagccgcc aagccgaaac
     7621 acgagcgaaa cggcaaaggc aaagatgcca aaaaaccggc gaaacctgcc gcacctgccg
     7681 tgccgcaacc cgtggtcagc gcggaagaac aggcgcaacg cgatgaagaa gcacgccgtg
     7741 ccgccgcact tcgcgcccac caggaagccc tgttgaaaga gaaacaggaa cgccaggcac
     7801 gccgcgaagc catgaaacaa caggcagaac aacaggcaaa agccgcacag gaagccaaaa
     7861 ccggcagaca gcgtcccgcc aaacctgccg agaaaccgca ggcagccgcg ccggccgtcg
     7921 aaaacaaacc tgtcaatccg gcaaaagcga aaaaagaaga ccgccgcaac cgcgatgacg
     7981 aaggtcaagg ccgcaacgcc aaaggcaaag gcggcaaagg tggtcgcgac cgcaacaatg
     8041 cgcgcaatgg cgacgacgag cgcgtacgcg tcggcaaaaa aggcaaaaaa ctcaaactcg
     8101 agccgaacca acacgccttc caagcgccga ccgaacctgt cgtacacgag gttttggttc
     8161 ccgaaaccat taccgttgcc gatttggcac acaaaatggc ggtcaaaggc gtggaagtgg
     8221 tcaaagccct gatgaagatg ggcatgatgg taaccatcaa ccaatccatc gaccaagaca
     8281 ccgccctgat tgtggtggaa gaactcggac atatcggcaa acctgccgca gcggacgatc
     8341 cggaagcatt cttggacgag ggcgcggaag cggtggaagc cgaagcattg ccgcgtccgc
     8401 cggtggttac cgtgatgggg cacgtcgacc acggtaaaac ctcgctgctg gactacatcc
     8461 gccgcgccaa agtggtgcag ggcgaagcag gcggcattac gcagcacatc ggcgcgtacc
     8521 acgtcaaaac gccgcgcggc gtgattacct tcttggatac cccgggccac gaagcgttta
     8581 ccgccatgcg cgcacgcggc gcgaaagcga ccgacatcgt gattctcgtg gttgccgcag
     8641 acgacggcgt gatgccgcaa accatcgaag cgattgccca cgccaaagcg gcaggcgtac
     8701 cgattgtggt tgccgtcaat aaaatcgaca aagaagccgc caaccccgaa cgcatccgtc
     8761 aggaactgac ccaacacgaa gtcatccccg acgattgggg cggcacggtt caattcatcg
     8821 atgtttccgc caaaaaaggg acaaacatcg acgcgctgct cgaagccgtc ttgctcgaag
     8881 ccgaagtttt agaactggcc gccccggtcg atgcgcccgc caaaggcatc atcgtcgagg
     8941 ctcgtttgga caaagggcgc ggcgcggttg ccacattgct ggttcaaagc ggtacgctga
     9001 aaaaaggcga catgctgctg gccggtacgg cattcggcaa aatccgcgcg atggtcgatg
     9061 aaaacggcaa atccattacc gaagccggcc cgtccatccc ggtcgaaatc ctcggcttgt
     9121 ccgacgtacc caatgcgggc gaagacgcga tggtattggc ggacgagaaa aaagcgcgcg
     9181 aaatcgccct cttccgccaa ggcaaatacc gcgacgtgcg ccttgccaaa cagcaggcgg
     9241 cgaagctgga aaatatgttc aacaatatgg gcgaaaccca ggcccaatct ttgtcggtca
     9301 tcatcaaggc agacgttcag ggctcttacg aggctttggc gggcagcctg aaaaaactgt
     9361 ccaccgacga ggtgaaagtg aacgtgttgc acagcggcgt gggcggcatt accgaatcgg
     9421 atgtcaacct tgccatcgct tcgggcgcgt tcattatcgg ctttaacgtg cgtgcagatg
     9481 cctcttcgcg caaacttgcc gaaaatgaaa acgtggaaat ccgctactac aacatcatct
     9541 acgatgccat cgacgacgtg aaagcggcga tgagcggtat gctttctccc gaagagaaag
     9601 aacagcttac cggcacggtc gaaatccgtc aggtcatcag cgtttccaaa gtcggcaaca
     9661 ttgcaggctg tatggttacc gacggcgtgg tcaaacgcga ttcccatatc cgcctcatcc
     9721 gcaacaacgt ggtcatccat accggcgagc tgtcgtcttt gaaacgctac aaagacgatg
     9781 ttaaagaagt ccgtatgggc ttcgagtgcg gcctgatgat taaaggcttc aatgaaatta
     9841 tggaaggcga ccaactggaa tgcttcgaca tcgtcgaagt tgcacgcacc ctgtgatttt
     9901 gatgcggaaa aatgccgtct gaagcgttca gacggcatac gaaacgggtt ctgtatcata
     9961 cagaacccgt tttttgtcgc aaatcggctt cagacagccc tcttgcctta tcccgatttg
    10021 aatctgactt gccatacaaa caggcttcag acggc
//
LOCUS       DAWFBO010000069         9797 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFBO010000069 DAWFBO010000000
VERSION     DAWFBO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.069"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(12..1307)
                     /locus_tag="WGO87_001191"
     CDS             complement(12..1307)
                     /locus_tag="WGO87_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0392550.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1433..2320)
                     /locus_tag="WGO87_001192"
     CDS             complement(1433..2320)
                     /locus_tag="WGO87_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0392551.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2320..3285)
                     /locus_tag="WGO87_001193"
     CDS             complement(2320..3285)
                     /locus_tag="WGO87_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0392552.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3301..4425)
                     /gene="potA"
                     /locus_tag="WGO87_001194"
     CDS             complement(3301..4425)
                     /gene="potA"
                     /locus_tag="WGO87_001194"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0392553.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4620..4727)
                     /locus_tag="WGO87_001195"
                     /pseudo
     CDS             complement(4620..4727)
                     /locus_tag="WGO87_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="WGO87_001196"
     CDS             complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="WGO87_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0392554.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5298..6233)
                     /gene="secF"
                     /locus_tag="WGO87_001197"
     CDS             complement(5298..6233)
                     /gene="secF"
                     /locus_tag="WGO87_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0392555.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6237..8090)
                     /gene="secD"
                     /locus_tag="WGO87_001198"
     CDS             complement(6237..8090)
                     /gene="secD"
                     /locus_tag="WGO87_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0392556.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRVGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="WGO87_001199"
     CDS             complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="WGO87_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0392557.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8696..>9797
                     /locus_tag="WGO87_001200"
     CDS             8696..>9797
                     /locus_tag="WGO87_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0392558.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAVAEYRLNRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRAFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2511 a         2581 c         2429 g         2276 t
ORIGIN      
        1 gcctgcgacg cttaacggct gtctttccac tgataatatt tcgagccgag gaaagaaccg
       61 agtttgcgca aaaacggttg caggataata ttcggctggc gcaaccgctc aaacggcttc
      121 agacggcatt cgtcccctaa aattgcttcg gcaaccgcca gacctgcaat gcctgttatc
      181 gccatcccgt gtccggaata accttgtgca taaaaaacat ttgcggccaa acgtccgaaa
      241 tgcgggacaa ggttggcggt aatgtcgcac tccccgcccc acgaatattc gattttgaca
      301 tcgtcaagct gcggaaaaac tttaagcata tcttggcgga caagctcggt catacgcgcg
      361 ggattgtcga taaactcgtt atccttaccg ccgaaaagca gtctgccgtc cgcgctgagg
      421 cggtaataat ccaaaatatg gcggttgtcg catactgcca tattgttacg gataagccct
      481 tttgcgcgcg cccccaaggg ttcggtcgca ataataaagg tgctaacggc aatcgccttg
      541 cgttccaaag gctggaatat cgggttcaaa cctgcatagg tattgacggc atacaccaga
      601 tttttgcatt cgacgctgcc ttcgggggtg taaaccagcc aaccgttttg ataaggttca
      661 atgcgtatca tgggagaatg ctcaaaaatc ttcgtaccag cttcggcagc ggcgcgggcg
      721 atgcccaacg tgtaattgag cggatggaga tgcccggaca agggatcgaa ctgtgcgcct
      781 tggtacatat cgctgtcaag ctgctgcttc agttcagtgt tatcccagag ttgataatga
      841 gttgcaccgt aatatttttg ggcgtgctca tgccattgtt gcaattcttc ccaatgctgc
      901 gaacggatgg caaccgtggc ataaccgcgc tgccaatcgc aatcaatggc atgtttgcgg
      961 acgcgttcgt ccaccagttc gaccgcctgc aaagactgtt gccaaaacca ttgcgcttgc
     1021 tccaaaccga cctgtttttc aatttcctcc ataccgcagg cgtaatcgct gataacctgc
     1081 ccgccactcc gtcccgacgc gccgaaaccg atacgcgcgg cttccaacac aaccgtttca
     1141 tgtccctgct ccgccaaggg caatgcagtg cacaaaccac tcaatccgcc gccgatgata
     1201 caggtatcgg ttttcagacg gcattgaagt ttcggataaa cagtatgagg attaaccgaa
     1261 ctgaaataat aagaaggcag atattcttga aaatcagggc gaatcattgt gtttgcttta
     1321 tcgggtatat tttcggacgg aatgatacag gctgtcgagc catatcgtcc aaacagaaaa
     1381 tcggttgaag aaaacaggct gacccagtca tgcggtcagc ctgccttatt aattaatttg
     1441 ctttctcggc agccaatttt tcttggcggt aggcttctgc cgcttctcgg tcacgcttgg
     1501 ttgcctgcct catcatccaa taattgacga tgatgaccaa tgttccgatg atgccgatca
     1561 ggatggttgc caaaacgttc atctgcggat caagacccag cttgattttg gagaaaatca
     1621 cctgcggcaa tgtggatgaa ccggggccgg agaggaatga ggtaatcacc aaatcatcca
     1681 aagacagggt aatgccgagc agaaagcctg aagcgatggc aggggcaatc aaaggcaaag
     1741 tgatgacaaa aaagattttc agcggacgcg cgcccaaatc cattgcggct tcttcgagcg
     1801 attggtcaag ctcaaccaga cgcgaacgga taacaacggt aatgtatgcc atgcacagcg
     1861 tcgtatgtcc taagaaaata gtgaaaaagc cacgatcgaa gtagagatgt tgtaaccatt
     1921 cgctgccctg caaaaatatc tgtacctgaa taatcagcag cagcatagac agaccggtaa
     1981 tcacgtcggg catcaccata ggtgcggaaa tcataccggc gaacaaggta ctgccgcgaa
     2041 aacgtttaat ccgcgccatc gcatagcctg ccagcgtgcc caaaacgacg gcggcaagcg
     2101 aagacacaac ggcaatccgc agcgacagcc aagcggcttc caagatggtg tcgttttcca
     2161 gcaatgcgcc gtaccacttg gtcgaaaagc cgccccaaac ggttaccagc ttggattcgt
     2221 taaacgaata gatgaccaaa acaaccagcg ggatatacag aaacgccagc gacagtgcca
     2281 acatcagttt caagaaccaa gataatttgg atttctgcat tatttggctc cttcttccaa
     2341 ttcgcggttt tcataatgct gaaacagggc aatcggcacg accagcagcg cgaccatcac
     2401 gacggcgacg gcggaagcca gcggccagtt gttttgatcg aagaacgcct gccacaagac
     2461 tttaccaatc atcaggtttt ccgaaccgcc gaccagctcg ggaatgacga actcgccgac
     2521 agcagggacg aaaaccagca tggagcctgc aataatgccg gttttcgaca aaggcagggt
     2581 aatcgtcaag aacgatttga ccggccccgc gcccaaatcg gaagccgctt caagcaggcg
     2641 gttgtcgagt ttcaccagtt gcgtgtatag cggcagaatc ataaacggca gataggcgta
     2701 aaccatcacc aaattgagcg aaaaggcatt gtagaacaaa tccaaaggct cgctgataat
     2761 acccatttta atcaacaggt tgtttacaat accgttatgc ccgagcagac ccatccacgc
     2821 atagacgcgc aacaggaacg atgtccaaaa gggcagcata atggcaagca gcaaaccatt
     2881 gcggacagaa ggattggcac gagaaatcgc ataggcggtc ggataaccga ccaacaggca
     2941 aatcactgtc gtcatcaatg cggttttgat tgaagaccag taggtcatca gatagatatt
     3001 gctgttttca ctgtcgccga acgggttaag ggtatgccaa aaattttgga aaatatcggc
     3061 atagttttga taactgatgg tgatattcag acgacctaaa tcttcgtcga tgctggtcag
     3121 cggcgtaaac ggcggaatgg cgatttcttg ttcggcaaag ctgattttca gcacgatggc
     3181 gaacggaatc agaaacagca ccaaaagcca aatatacggt acggcaatca ccacacgctg
     3241 ccccgggcgg cggaacagtt tgtttttcag tttattaagg ttcattgcat tccccttaaa
     3301 tcaacggaac agtggcgtag gctggttttc cggccagctg atatacacgg tttcgtccca
     3361 agttggcggt gtaatgttgc gtacatacca gtaaggtgca ggaacttggc ttttgacgac
     3421 gcgcccgttg gcgagtttga tatggtatat ggcaaagctg cccaaatagg cgatttcttt
     3481 taccgtgcct ttcgcccagt tgtagtcgcc caaatattcg ggtttttctt tatataaatc
     3541 aatatcctct ggtcgaatac taacccaaag gtcctgctcg ctcggaccac ccaaaccgtg
     3601 atcgatgcgg acgtggtttt ccaaaccttc gcattcgata acggcatagt cggcatgatc
     3661 ttcaatcacc acaccgtcaa agatgttggt ttcgccgata aactcggcag tgaagcggct
     3721 gttgggatag tcgtacacgt cgctgggtgt gccgacttgc tgcaactgac cgtcagacat
     3781 aatggcgatg cgggtcgcca tcgtcatcgc ctcttcttgg tcgtgcgtaa ccataataca
     3841 ggttacgccg acttgttcca gcgtattgac caactcaagc tgggtttgtt ggcgcagttt
     3901 tttgtccaat gcaccgaggg gctcatccag cagtagaatt ttcggacgtt ttgccagact
     3961 gcgtgccaaa gcaatgcgct gctgctgacc accagaaagc tggtgaggtt tgcgtttggc
     4021 aaatttggtc atctgaacca ggcggagcat ttcttcgacg cgcgcggcga tttcgccttt
     4081 aggcattttg tcctgtttca gaccaaaggc aatgttttgt tctacggtca tatgcggaaa
     4141 aagcgcgtaa ctttggaaca tcatattgat ggggcggtca tagggtgcaa gtttggtaat
     4201 atcctgacca tcaaggataa ttttcccctg attggggctt tccatacccg ccagcatacg
     4261 cagcagcgtg gattttccgc tgccggaact gcccaaaagg gcgaagattt cgtgttgata
     4321 aatgtccaag tcgatgttat cgacagcgta attgtcacca aactttttca ccaagccttg
     4381 gatttgcaga taaggtttgg ctgaagacgc agtggttgcg gtcataatgg caatactcca
     4441 ataaaaaaac gagtaccggc aaaacggatt ttcgaatggg tgataaaaag ctgtttgatt
     4501 gctggcggga gttaaacgtt tgatgccgtc tgaaactctt gtaaagcgca cgggcagcat
     4561 gaaatggaac aagattccaa agaactttat attatattag tttatgcggt tttcgggcaa
     4621 tatagtggat taaatttaaa ccagtacagc gttgccttgc cttgccgtac tatttgtact
     4681 gtctgcggct tcgtcgcctt gtcctgattt aaatttaatc cactataaat atggatttga
     4741 gcttgtcgga aagcaacaga aaagaaaaca ccgcccattt ttctgggcgg tgtcggaaag
     4801 cgtaattatt tacgcagacc caagcgggta atcaacgcgc gatacgtatc gggctgggta
     4861 cggcgcaagt aggccagcag gcggcggcgt tggctgacca ttttcaacag gccgcgacgg
     4921 ctgtggtggt cttttgggtt ggctttgaag tggggggtca ggtcgttgat gcggaaagtc
     4981 aacagagcga cttgtacttc ggaagagccg gtgtcgcctt ctttgcgttg gaaatcttta
     5041 acgatttgtg ctttttgttc tacggtcagt gccataatga aaactccaaa aatataagaa
     5101 tccccatagg gattcagaca agtttgccaa gcctgaagac aacggcaaac tccctatgcc
     5161 caagatagga caacgtggca ttatgacaca attcccgcat ttctgcacaa tattttaaga
     5221 ccgtggaagt aaccgttaaa aatgccgtct gaagcattgt ctgcttgaga cggcaatgtt
     5281 cagacggcat atgcgcttca aaccacgatt tcttctttct tcttcggttc tttaccgata
     5341 ttgtcgcggc tcaaaccgaa cattagcaga agcgggctgg caaccaatac ggaagaataa
     5401 atgccgaaca cgatgccaat ggtcaacgcc atagaaaagc cgtgcaaggc cgcaccgccg
     5461 aacaccagca tagaaacaac catcgcctcg gtcgaaccgt gggtaatgat ggtgcggctc
     5521 atcgttgcgg taatcgcgtt gtcgatgact tccggcacgg catgtccgcg catcgccggc
     5581 ttgcggaagt tttcacggat acggtcgaag acgacgacgg attcgttcac agaatagccc
     5641 aatacggcaa ggatacccgc caagacggtc agcgaaaatt cccattggaa gaaggcaaag
     5701 cagccgagaa taatcacgat gtcgtgcata ttggcgataa tggcagatac ggcaaaacgc
     5761 cattcaaaac gcatcgacag gtaaataatg atgccgataa cgacaaaacc taaagccatc
     5821 aatccattac ttaccaattc ctcaccgact tgcgggccga taaattcgac ttggcgcaag
     5881 gtaacgtcgg gactgtcttt tttcagcaaa tccataacct gattggacaa ctgtgcggaa
     5941 gtaacacctt ctttgttcgg caggcggatc atgatgtgtt tgttcgtacc caatgcctga
     6001 acctgtacat cacctatttt cagcgtatcg aggcgttcgc gcatcttatt gacatccgca
     6061 ccctgctgat attggacttc cattaccgta ccgccggtaa attcgacaga gaaattcaga
     6121 cctctggtaa ccaaaaagaa tacggcggca ataaaggtaa ccaacgaaat aaaggtcgtc
     6181 agtttgccgt agctcataaa cggaatatcg cgtttgattt taaagagttc catagcttac
     6241 tccttgcctc ctgccatttc ggctttcggc ttccacaccg aaccgatgga aatattctgc
     6301 aatttgcgtc tgcgtccgta ccacagattg accaacgcac ggaatacgac gacggacgaa
     6361 tacatcgaag tcagaatacc caaacagtgt acgaccgcaa aaccgcgtac cgggccggaa
     6421 ccgaatacca aaagcgcgat accggcaatc agcgaagtca ggttggaatc gacaatggtc
     6481 gcccatgcgt gttggaaacc gagattgatt gcctgctgcg gcggcacgcc gacacgcaat
     6541 tcttcgcgga tacgttcgtt aatcaagacg ttggagtcga ttgccatacc caaagtcaac
     6601 gccagcgcgg ccatacccgg taacgtcaac gttgcctgca tagcagacaa aataccgatt
     6661 aggaacagta tgttggcact caatgcaatg gtagaaaaga aacccatcag acgatagtaa
     6721 accaccatga atgcagcaac gatggcaaaa ccccataaag tcgaatggaa gcctttttcg
     6781 atgttctcct tacccaaaga cggaccgatg gtacgttctt cgacaatctg catcggcgcg
     6841 gcaagagaac cggcacgcaa cagcaaagac gtatcattgg cttcggctgt cgtcatgctt
     6901 ccggaaattt ccacgcgtcc gccggtaatg gcagtacgga taaccggcgc ggttacaacc
     6961 tcggattttc cttggtcgat caaaaccatc gccatgcgtt tgccgacatt tgcggcagtc
     7021 agttcgccga aaatgctgcc gcccgcgctg tccaagctca gactgacggc aggtgcgccc
     7081 atttggtcga aactcggttg cgcatcgttg atgttgtcgc ccgtcagctc gacctgtttg
     7141 ctgattagca gagtttcggg acgatctccg ccgcttgaaa gcagctcata accgctcggc
     7201 acgttgcctt ccaatgcctc gcgcaacttg gcaggatcgt cctccaccat acgcaattcc
     7261 aaagtcgcgg tacggccgat gatgtctttt gccttggcag tatcctgaac gcccggaagc
     7321 tgcacgacga tacggtctgc acctgactgc tggatgacgg gttcggccac gcccaactcg
     7381 ttcacacggt tgtgcagggt agtgatgttc tgtttgaccg catcggaacg cactttattg
     7441 accgcctctt ccgaaagcgt caagacgata ttgctgccgt ctgaattcag cgttgcttca
     7501 ggaaacagct tgcgcaactg cggcagtgcc ttttgcacat cacctgcatc ctgcaaaggg
     7561 acggtcaggc tgtttccagc ctgacgcacc gtgccgctgc ggattttttc gcggcgcagt
     7621 tcgcggcgga tgtcgcccga ataacgttca aacgttttct gcatcgccgc tttcatatcg
     7681 acctgcatgg tgaaatgcac gccgccgcgc aagtccaaac ccaaaaacat cggattggct
     7741 ttgattttcg ccatccattc ggggctgtcc gccaacaggt tgagcgcggt aatataccct
     7801 tcgcccaaag tgttttcgat gacgtcgcgc gctttaagct gcgtttctgt gtctttgaaa
     7861 cgcactttca gtgaattgtc cacaacaaac atcccgtcgg tctgaatacc tgcgtttttc
     7921 agcgcggcat ccactttgaa ttgagtctgt tcgttgatga tgatggcttg tcggttggtc
     7981 gatacctgca cggcgggtgt ttcgccgaat aggttgggca gcgaatacac tgcggcaacc
     8041 gcaatcgtga acacaatcag cagatatttc cataaaggat aacggttcat cattgttcct
     8101 taatggttgg aaccccaccc tttcggtggt gtcggaatcg ggctatttca gaagaggcaa
     8161 aaacccttcc cagccaggca agaccggaag gcggcatctt gaatatgccg ccctgcgtgt
     8221 cggaacatgg tcaagccttc ggttggaatt caaaacaaag tgccgcattc gggctttcca
     8281 gatgcggctt gtcggcacaa atcaatcgac ttttgcggca atcgcattgc gttctacttc
     8341 gacctcgatt tttgtaccct gtccgatatc cacggtaaaa aactgttcgc cgactctggt
     8401 taccttaccc ttgaaacctg ccgccaagac cactttgtcg ccgactttca aggcggcaag
     8461 cattgcctga tgcgctttga atttcttttg ctgcggacgc atgatcagga agtagaacac
     8521 caccataatc aacactaaag gagcaaattg tgcaacagct tgattcataa tttatccgtt
     8581 ctttctaata tggttgaaaa tcgagagggg tatataataa cataagaccg taaacaatat
     8641 atcgggtttg ccgtccgtac cgaccgtata ccgcagcctg cccgtccaca aacccatgtc
     8701 cttgacccgc ctaatcttga aattctatgc actgttgcgc ctttttttgg gcaaaaacgc
     8761 ccgcaccgca tggatttcgc atcccgcctg tgccgggcac gaacccggcg caaaccatcc
     8821 cgattcgccc gaccgcatcc tctgcatcga gcaggcattg cgccgcgccg gtatttggca
     8881 gcacctccaa accatagagg cggaagaaat cagcgatacg cgcctcgcac ttgtccactc
     8941 gagcaaatat ctgaaccgtt tggaatcttg cctgccccaa aaaggcaaga tttcccgcct
     9001 ggataacgac actgcaatca gcacaggctc gctgtctgcc gcacgctttg ccgccggttc
     9061 ggcagttcag gcagtcgaca tggtcatgaa ccgtaaagca tggcatgcct tttgcgccgc
     9121 ccgcccgccc ggacaccatg cgggcagcgg caaagccggc ggattctgcc tgctgaacaa
     9181 cgttgccgcc ggcgtcatgc atgccgttgc cgaataccgg ctgaaccgca tcgccgtcat
     9241 cgatttcgat gtacactacg gcgacggtac ggcagaaata ttcaaagacg atccgcgcat
     9301 cctgtttttc aacctgtttg aaaccgacct tttccccttc cccgaaaaca acgatatgcc
     9361 cgacggcggc aatatggtgc acctgccctt gccgccggga acgggcagcc gcgcattccg
     9421 cgaagccgtc cgcagacagt ggctgccccg gcttgccgca ttcaaacccg aactggtgct
     9481 gctgtcggca ggattcgacg cacaccgtct ggacgaatcg ggcaggctta acctgcacga
     9541 ggcggatttt gcctggctga cacacaaaat cattcagacg gcatcgggct gccctggcaa
     9601 aatcgtatcc gtactcgaag gcggctacac ccttgaacct ctggcacaat ctgctgccga
     9661 gcatatccgc gtgctggcag ggctgggcaa atccgatgcc gcaaccgcct atcagaaaac
     9721 actggaccca acaaaaaaac ggtttgccaa accgaaaacc ggacaggtgc gccaacctac
     9781 ccaatccgac cgttacg
//
LOCUS       DAWFBO010000070         9770 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFBO010000070 DAWFBO010000000
VERSION     DAWFBO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.070"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(313..546)
                     /locus_tag="WGO87_001201"
     CDS             complement(313..546)
                     /locus_tag="WGO87_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0392559.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(712..912)
                     /locus_tag="WGO87_001202"
     CDS             complement(712..912)
                     /locus_tag="WGO87_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0392560.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="WGO87_001203"
     CDS             complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="WGO87_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0392561.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGSVDLDRG
                     CITALGKGDKQRMIPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2011..2451)
                     /locus_tag="WGO87_001204"
     CDS             complement(2011..2451)
                     /locus_tag="WGO87_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0392562.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2772..3710
                     /locus_tag="WGO87_001205"
     CDS             2772..3710
                     /locus_tag="WGO87_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0392563.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKKLLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3839..4132
                     /gene="hfq"
                     /locus_tag="WGO87_001206"
     CDS             3839..4132
                     /gene="hfq"
                     /locus_tag="WGO87_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0392564.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4217..4783
                     /locus_tag="WGO87_001207"
     CDS             4217..4783
                     /locus_tag="WGO87_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0392565.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNNPPYLLAFEKLRQK"
     gene            4837..5211
                     /locus_tag="WGO87_001208"
     CDS             4837..5211
                     /locus_tag="WGO87_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0392566.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5221..5715
                     /gene="folK"
                     /locus_tag="WGO87_001209"
     CDS             5221..5715
                     /gene="folK"
                     /locus_tag="WGO87_001209"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0392567.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPCAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(5842..6321)
                     /locus_tag="WGO87_001210"
     CDS             complement(5842..6321)
                     /locus_tag="WGO87_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0392568.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(6541..7278)
                     /gene="ubiE"
                     /locus_tag="WGO87_001211"
     CDS             complement(6541..7278)
                     /gene="ubiE"
                     /locus_tag="WGO87_001211"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0392569.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7321..7689)
                     /locus_tag="WGO87_001212"
     CDS             complement(7321..7689)
                     /locus_tag="WGO87_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0392570.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7809..9428
                     /gene="mnmC"
                     /locus_tag="WGO87_001213"
     CDS             7809..9428
                     /gene="mnmC"
                     /locus_tag="WGO87_001213"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0392571.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAQLNPTLAESLFAANPPPQKHQGHAAIRCDSPDHLPVVGAL
                     GNIAAMQQTYAKLALDKNYRIDSPCPYLPNAYANTAHGTRGLATAPICAAAVAAEILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9425..9574)
                     /locus_tag="WGO87_001214"
     CDS             complement(9425..9574)
                     /locus_tag="WGO87_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392572.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT         2606 a         2979 c         1966 g         2219 t
ORIGIN      
        1 ctaccgatta cacaaccaca tatcatcttt acacaatcat gcttccatca acagcaaaac
       61 atgatatgat tgctgctaat aacatctcac aataaatttt ctaattttta ttgaaaaaat
      121 caataaataa gaattctccc aaccgacaaa taaaataaag aaagggtcaa tatgcaacat
      181 cgaagaagac tggcaattta ccaggcatcc aaacgtgctt cctttaccgg caggtcatcc
      241 gccccgcaaa aacgtaaaga acgttgattt gaaaaaaatg ccgcctgaag tcctgcttca
      301 gacggcattt ttttaccgtt cgagaaactg tttcaacctg tcctcatcca aaaaccagtg
      361 gcagatttcc tcatctcccg ccaacaaaac gggaaccagc tcattgtatt tttcttccaa
      421 aacaggattt tcatccacat cgaccacttc cagcccgaac ccgtattcat tttgaaaagg
      481 tttgagtgca tcgcgcattt tgtggcacaa gctgcaatat tcacgaaaca tcaaggtcaa
      541 tttcatccgc gtttccttat ctgtcaattt gcacacgcca aagccttaga cgcagcagaa
      601 tcatggtcta tttgggaaaa aacaatgttt tcaaggaaga tgatactcaa gtcctgccaa
      661 aaacagcaaa aatgccgtct gaacagttca gacggcattt ccaaaaccgt tttacgcttg
      721 aacattgata cccgccgtaa ccgtcggttg cgcattgtgc gccgtcagaa acgaagcggc
      781 aagctcccca cagcagccgc agcagctcgc tacgcccaat tgcgactgca aatcgcccat
      841 tgtggtcgcg ccggcggcga tggtttcctt gatttgatgg tcggtaacgg cattgcagat
      901 gcagacaaac attttgtgct ccgtgtgttc tcaaactatc gtaccgatag cggcttttat
      961 tatcgtatgc aaatataaat aaaaacggtt cgcattgcaa ggtcggcata cacggtttgt
     1021 cttggtaatt ttttatcaag ttttcatttc cgattgattt cttattaaac cgaagcagaa
     1081 gcctcagttt cgggaatggt gttccttcac tacaccctgc aaccatacgt tggcaacatg
     1141 ggtataaatc tgcgtcgtat tcaaatcggc atgtcccaac atatcctgaa ccacgcgcaa
     1201 atccaagccg tgccgcacca gatgcgtggc aaaggcgtgg cgcaggctgt gcgggctgat
     1261 gtgcccgatg cctgcctgac ttgcatattc tttgacaatc atccatgcca actgacggga
     1321 aatgcccgtc tttttctgac tgacaaacaa tgcgtcgcaa ttcctgcctt tcagcagaag
     1381 tgggcgtgcc tccgtataat agcgttccac ccaatacgcc gactcctgcc ccatcgggat
     1441 catcctctgc ttgtcgccct tgcccagcgc ggtaatacag cccctgtcca aatccacact
     1501 gccgaaattc agcccgaccg cctcgctgac gcgcaagccg gtcgcgtaca tcaattcgag
     1561 cagtgccttg tcccgcaaac cgtgtggcgt atcagtatcc ggagtggcaa gcagccggga
     1621 aatctgctgt tctgtaatca gtgtcggaat attcttgtcg attttgggcg gtttcagcaa
     1681 acgggtggga ttgtccgtcc ttatgccttc acgctccatc catatataca ggcgtttgca
     1741 cgccgataat gcgcgcgcct gcgaactccg ttgctctccg tcaacataaa ccgccgccgc
     1801 caaatccgct tcgtccgcat ccttcagcat tctgcccgat tgggacaggc ggcgggcgat
     1861 tttttccaaa tcgcgccggt aaccgtttaa agtattctga ctgagccgcc tgtccaacca
     1921 cagcgtttca agcagcctgt cgatcaaacc ttcttccata ccgttccaaa caaatgccgt
     1981 ctgaatcttc ttcagacggc atggttcaca ttatcgggaa agcgtttcca atacttcctg
     2041 cgcgtgaccc gccactttga ctttccgcca ttcatgggcg atttctccat ccttattcaa
     2101 gacgaacgta ctgcgctcga tacctaacga ctctttcccg tacagtttct tcaatttgat
     2161 gacatcaaac aggcggcaca ctgtttcatc cttgtcgctc aacagctcga accggaaacc
     2221 ctgcttggcg caaaaattct gatgcgcctt tacgccgtcg cgggaaatac cgaccacggt
     2281 ataacccaat gcctcaaact gttccaaacg cgcattgaaa tccaagcctt ccgtcgtaca
     2341 gcccggcgta ctgtctttcg gataaaaata cacgaccaaa ggcagatgtt ctgccgaatg
     2401 aaaatccgca ccgctgctcg aaggcagggt aaattcatat ttcacatcca tagtcctact
     2461 cccgatattc ccattattca aaacgacacg cagacgcacc gccgcaattg ccaaaccaac
     2521 cccgattcta ccgccccaaa ggacagggat tcaaccgccg gaaacatcca aaccgacaca
     2581 cgacggcatg aaaaatatcc atgtcaaacc acaaaatatg ttccgattta aaaacagaat
     2641 gttataaaac ccaatcccca aaacacaaca agaccgcccg cctacgggca gtctcctgtc
     2701 agacgacata ctttacagat ggctgttttt tcaacaaaat aacgccaata ctcaaaaata
     2761 tggaatcaaa aatgtccatc catactctga aacgcctgcc ctcatcgctg ctgctcggtc
     2821 tctgcctttc cctgccgtca gcccaccttt ttgccgacaa cgacatttta gggcaatttt
     2881 tagaacagaa catgcttacc tcctccgatc cgatagaaat attcgccgaa agcacgatac
     2941 accccaccaa cacccaagcc attacaggcg gtctgattct ctcctcacag tctgccctgg
     3001 tcgtcaacaa caaaaccgga cagatactgt atcagaaaaa cgccgacagg attatgccca
     3061 tcgcctccat ttccaaactg atgagcgcga tggtcgtttt ggatgcaaac ttggacatga
     3121 acgaaaccgt taccattacg cccgacgaaa tcgaccgcat caaagggacc ggcagccgtc
     3181 ttgccatagg tacggcactt acacgcaaaa aactgctgca cctgagcctg atgagcagcg
     3241 aaaaccgcgc cacccatgca ttgggcagaa cctaccccgg cggcatgggc gcatttgtcg
     3301 ccgccatgaa ccgcaaagcc caaagcctcg gtatgtacgg cagccgcttt tacgaaccga
     3361 ccggactcaa cttccaaaac gtttctaccg ccaaagacct gagccttatg gtcaacgccg
     3421 ccgcccaata tccgcaaatc cgcaccaact cgacttccaa ctacgcctcg gtacagacca
     3481 aaaacgggca gcagaactac aaaaactcca atgccctggt cagagaaggc atgtggaaca
     3541 tcgaattgca gaaaaccggc tacatacgcg aagcaggcag gtctatggtt gtcaaagcca
     3601 acattcaaaa ccaacccgtt accatcgtat tgctgaactc gcccacatcc gccacacgcg
     3661 tcaacgacgc ccgcaaaatc gaatcgtgga tgctgcagca acgctcctga catacaaatg
     3721 cccggcggaa aaccgcccaa acacaaaaaa aattggtaat ttttcctatt caaggctaca
     3781 attcgacacg cacagggcgt ctgcccgatt tcacaatcat aataacggag ttaaaaatat
     3841 gacagctaaa ggacaaatgt tgcaagatcc cttcctgaac gccctgcgta aagagcacgt
     3901 tccggtttcg atttacttag ttaacggtat caaattacaa ggtcaggttg agtctttcga
     3961 tcaatacgtt gttctcctga gaaacacttc cgtcacccaa atggtttaca aacacgccat
     4021 ttccaccatc gtaccggcac gctccgtcaa cctacaacat gaaaacagac cccaagccgc
     4081 accgacttcg accctcgtcc aagtggaaac cgtccagcag cctgccgaat aatccgcacg
     4141 aagcatgacg tgtcatatct ttcaatacct taccggacaa cggtaaggta tttttatttt
     4201 cagacagcat ttaaaaatgt tattgcaaaa catccttcca ttcgcccatt gccttttgcg
     4261 gaaggcactt cccgaaggtg gcaatgcttt ggacggcacc gccggcaacg gacacgacac
     4321 ccttttcctc gcacaaaccg caggcatccg ggggaaagtg tgggcattcg acatccagcc
     4381 gcaagccctg agccgcactc gatgccgtct gaaagaagcg agatacgaga atgtgcaact
     4441 catcttagac ggacacgaaa acctgaaaca atatgtttca gaaccactaa atgccgccat
     4501 tttcaacttt ggctggctgc ccggcggaga taaaagcctt accacccata cggaaaccag
     4561 cattcccgcc ctttgcgcaa ccctctccct gctgaaagaa ggcggtatgc ttattgccgt
     4621 cctctatccg ggacacgaaa acggcaaaca ggaggcagaa gcaatcgaac aatgggcaaa
     4681 aaacctgcct caagaacagt ttgccgtttt gcgttacggc tttaccaacc ggaaaaacaa
     4741 cccgccctat ctgttggcat ttgaaaagtt acgtcaaaaa taaatgtttg cggtaaaata
     4801 agccgattct aacccttatt ttgatacgag ccgaccatgc agtatctgat tgtcaaatac
     4861 agccatcaaa tcttcgttac catcaccatt ttggtattca acatccgtgt tttcctactt
     4921 tggaaaaatc cagaaaagcc cttggtcggc ttttggaagg cactgcccca ccttaacgac
     4981 accatgctgc tgtttacggg attgtggctg atgaaaatta cccatttctc cccgttcaac
     5041 gcgccttggc tcggtacaaa aatcctgctt ctgctcgcct atatcgcatt gggtatgatg
     5101 atgatgcgcg cccgtccgcg ttcgaccaag ttctacaccg tttacctgct cgccatgtgt
     5161 tgcgtcgcct gcatcgttta ccttgccaaa accaaagtcc tgcctttctg aaacaccgtt
     5221 atgaacaaca gacattttgc cgtcatcgcc ctgggcagta atcttgaaaa ccctgcccaa
     5281 caggtacgcg ccgcattgga cacgctgtcg tcccatcctg acatccggct tgaacaggct
     5341 tcctcactgt atatgaccgc ccccgtcggt tacgacaatc agcccgattt cgtcaatgcc
     5401 gtctgcaccg tttccaccac tctggacggc attgccctgc ttgccgaact caaccgtatc
     5461 gaagccgatt tcggacgcga acgcagcttc cgcaacgcgc cgcgcacatt ggatttggac
     5521 attatcgact ttgacggcat ctccagcgac gacccccgac tcaccctgcc gcatccgtgc
     5581 gcgcacgaac gcagtttcgt catacgccct ttggcagaaa tcctccctga ttttgtttta
     5641 ggcaaacacg gaaaggttgc cgaattgtca aaacggctgg gcaatcaagg catccgtctt
     5701 ttaccggata agtaattccg cacgcggatg ccgtctgaat gccggtttca gacggcattt
     5761 ttcctttgcc gccaacacgc gtgcaaaaaa atcgcccctt ggaaaagggg cgcaaaagga
     5821 acacaaacca ctaccaaaac tttaaatctg caacattgcc tgccgcatac tgtatccgac
     5881 aggatataaa gccctcacta aatcgtttcg agaaatccaa acttcttcat cgccgacaga
     5941 aaatctgcct ttctccggca ccagctccaa caaaaacggt tgaaccgccg tatgcagcct
     6001 gtccttacac cgccccagct ttctggacaa ggcggacagg gcacgcttat agacactatc
     6061 cttgcggtca agttcccgcg cactgccgac ccagctcaaa cgaaggttgc ggctttcatc
     6121 ctcaatcagc aaaatgcctg ttttgacttc ccccacctct ggctcgcatg aaagcgaaat
     6181 ataatatttg ccgtcgatgt tgacatacgc cgcctcatgc acctgacagt tgcagccgga
     6241 agtggcaagc agcgaataaa cagaatcccg atgcagtttc aaaatatcaa gggaaatctg
     6301 tttgaaaata ctttccggca tttttccctc ctttcgtctg ctgcgttgtt tactggattg
     6361 actgcattat acagacatct cccaatcttg caaatgataa ttgttcctat tttttaaaaa
     6421 atttataaat tattgataga tatgtgaatt aattttaaga tttaaaatct ctttctcaaa
     6481 aaaacacaaa aatgccgtct gaaacgtgtt cagacggcat tggctgcaca gccagtccgt
     6541 ttaaaatttc acgcccttat gcagcgcaac gatccccgca ctcatattat gatagtccac
     6601 actgtcaaat ccggcatcca gcatcatttg ctttaaagtt tcctgatcgg ggtgcatacg
     6661 gatggattcg gcgagatact ggtagctgtc cgcatctttg gcaatcagtt tgcccatgac
     6721 cggcagcagc ttgaacgaat agaaatcata cgcgccttcg agcggcttgt agattttgga
     6781 aaactccaac accagcaacg tgccgccagg tttcaaaaca cgatacatct ctttcagcgc
     6841 ggcatcttta tgcgtcatgt tccgcaagcc gaacgccacg gaaactaagt tgaaataatt
     6901 gtcagggaaa ggcagttttt ccgcatcggc aagcgacacg ggcaaaatca tgccttcgtt
     6961 caacagacgg tcgcgcccga cggtcagcat agaggaatta atatcggtca gccaaacctc
     7021 gccttccttg ccgacccgtt tcgcccaacc gcgcgacaaa tcgcccgtac cgcccgcaat
     7081 gtccaacact ttatcgcctt ttttcaggtg cgccgtgttg atggtgaaat gcttccacac
     7141 cctgtgcagc cctgccgaca tgacatcatt cataatgtcg tagtttttgg cgacggagtg
     7201 gaacacttcc gccactttgc cggctttttc atcttcgttg accgtgctaa acccgaaatg
     7261 cgttttttgt ccgcccatct tctgaccacc tccctgatgc cgtcctccgg catcccaaac
     7321 ttaacaatct gattgctttt caaaacgcga cgcaccagcc gccgccaatt tgtccaaata
     7381 ttcctgccac atcgcatcgt atccgtatgc cagtctgtgc agatacgccc aatcgtaaag
     7441 accgctgtcg tgcccgtccg aaaaactgat tttcagcgcg tactgtccga caggctgcaa
     7501 atccgtaatt tgcacatctg ccttaccggt ctgcaaaaca tcctgtccca caccgtgtcc
     7561 gcgcacttcc gcactcggcg aatagacgcg taaaaattcg gcaggcagat ttttaggttc
     7621 gtccccataa gtcaaaacca acacggtacg gtttttcaac aaacggattt caacaggaat
     7681 attattcaaa aaacacctcg gttcggcata caatatccgg caggcgcagg cgtgccttca
     7741 aacggcgcgt attttaacag ctttatcccg ataccgatat tcaaacccac atttcagacg
     7801 gcattgatat ggacaatctc gtatgggacg gcattcccga catccgcaca ctcgaccaaa
     7861 ccatccgcaa acacgcacac ccgctcaacc tgattgtctg cctccccgat aatcagattc
     7921 ccgattttca aaccgcacaa gatgcttcgg acgcggaatg ccgtctgaag caccgtttgg
     7981 atcaggcaac gcagtgcctc cagttcgaca gcatcaacct catcgaacac atcctgcccg
     8041 atgtccgctt ctggctggtt cccccttcac gcacccgccg cctgcacgaa cacttccacc
     8101 acatttcctg gcagaccgaa gccatcccgc aaaccgaaag taagcccgac aaaccctggt
     8161 ttgcacttcc acaaacatcc gaacggcaaa aaccggaaca catcctcgtt atcggcgcgg
     8221 gcatatccgg cgcggcaacc gcccacgcct tagcatcata cggcatttcc gttaccgtat
     8281 tggaagcccg aaaagccgcc caagccgcca gcggcaaccg ccaagggctg ctctacgcca
     8341 aaatctcgcc gcacgacacc gaacaaaccg aactgctgct tgccggctac ggctacacca
     8401 aacgcctgct cggacacatc ctgcccgaat ccgacacttg gggcggcaac ggcatcatcc
     8461 acctcaatta cagccgcacc gaacaacaac gcaatcacga attgggtttg caaaaacacc
     8521 ataaccacct ctaccgcagc atcacgcagg cagaagccga aaaaatcgcc ggtatcccgc
     8581 tgtccgtccc atacgaccac ccttcatgcg gactctactg gcaacacggc gtatggctca
     8641 atccgcccgc attcgtccgc gccctcctcc gccatccgct gatcggactg cacgaagaca
     8701 caccattaac cgacatttcc cacgacgggg aaaagtggat tgcaagcacg ccaaacggca
     8761 catttaccgc cacacacatc atctactgca ccggtgcgaa cagcccctac ctacccgaaa
     8821 ccaacctcgc cgccctgcct ctcaggcaaa tacgcggaca aaccggcctc acaccgtcca
     8881 ccccgttttc cgaacaactg cgttgcgccg tttcaggcga aagctacatc agcccgtcgt
     8941 ggcacggact gcactgctac ggcgcgagtt ttattcccaa cagcagccat actggatgga
     9001 acgaagccga agaagcctca aaccgccaag cattggcaca acttaacccc acccttgccg
     9061 aatcattgtt tgccgctaac ccaccccccc aaaaacacca agggcacgcc gccatacgct
     9121 gcgacagccc cgaccatctg cccgtcgtcg gcgcactcgg caacattgcc gctatgcagc
     9181 agacctacgc caaactcgcg ctggacaaaa actaccgcat agattccccc tgcccgtacc
     9241 tgcccaatgc ctacgccaat accgcccacg gcacacgcgg gcttgccacc gcccccatct
     9301 gcgccgccgc cgttgccgcc gaaatcctag gcttgcccca tcccttttca caacgcctgc
     9361 gccacgccct acaccccaac cgcaccatca tccgcgccat cgtcagaagg aaggatctaa
     9421 ccccttaagc ctttgccgca tgccgtcccg accacagaaa atcatattca aataaacata
     9481 ccaatcctga cggtaaaata tcgaaaacac ccataacgtt ttcttcccaa aataaggagt
     9541 aaggaatgcc taacaaattg cctttgctgt ccaagatgaa tattccgtaa cattcgaaga
     9601 cttccttaac cctgccagcc gtttcggttt ctgtaccccc tgttagataa ggaaggaatg
     9661 aaaaagccct gttgattgta tgaagtaaag ggcgttaatt tttgttaatc ttcccttctc
     9721 aggaactcaa tatataagga tgttaatttt tatttccctt ctcgaggact
//
LOCUS       DAWFBO010000071         9642 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFBO010000071 DAWFBO010000000
VERSION     DAWFBO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.071"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..547)
                     /locus_tag="WGO87_001215"
     CDS             complement(<1..547)
                     /locus_tag="WGO87_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0392573.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIP"
     gene            complement(579..962)
                     /locus_tag="WGO87_001216"
     CDS             complement(579..962)
                     /locus_tag="WGO87_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0392574.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1331..2035
                     /locus_tag="WGO87_001217"
     CDS             1331..2035
                     /locus_tag="WGO87_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0392575.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASYGVEGTDSVA
                     PGVEEISISVNGTVQF"
     gene            complement(2100..2666)
                     /locus_tag="WGO87_001218"
     CDS             complement(2100..2666)
                     /locus_tag="WGO87_001218"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0392576.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2732..3147)
                     /locus_tag="WGO87_001219"
                     /pseudo
     CDS             complement(2732..3147)
                     /locus_tag="WGO87_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3209..4108)
                     /locus_tag="WGO87_001220"
     CDS             complement(3209..4108)
                     /locus_tag="WGO87_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0392577.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(4139..5596)
                     /gene="der"
                     /locus_tag="WGO87_001221"
     CDS             complement(4139..5596)
                     /gene="der"
                     /locus_tag="WGO87_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0392578.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5746..6375)
                     /locus_tag="WGO87_001222"
     CDS             complement(5746..6375)
                     /locus_tag="WGO87_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0392579.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQNEINAELAKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6376..7671)
                     /gene="hisS"
                     /locus_tag="WGO87_001223"
     CDS             complement(6376..7671)
                     /gene="hisS"
                     /locus_tag="WGO87_001223"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0392580.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRTQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(7798..8340)
                     /locus_tag="WGO87_001224"
     CDS             complement(7798..8340)
                     /locus_tag="WGO87_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0392581.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
     gene            complement(8736..9272)
                     /locus_tag="WGO87_001225"
     CDS             complement(8736..9272)
                     /locus_tag="WGO87_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392582.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9440..9640)
                     /locus_tag="WGO87_001226"
     CDS             complement(9440..9640)
                     /locus_tag="WGO87_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392583.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2254 a         2309 c         2456 g         2623 t
ORIGIN      
        1 ccggtatttt cttaccgtat tctgcgattg cctgtcggaa aatgccgatc aacctgccta
       61 taacggcatt ttcgccaaat tcgttcagac agttttccct aagtcgggca ggttcaaaat
      121 cagagtggtg ttcatacatt ttgatgagtg cgtcggcaag ggcatcgtcg tcgtcaacag
      181 gaaccaaata tccgttgccg tctgaaacaa tagattccgc accgccgcag cgtgtcgcaa
      241 tgacgggcaa tccttgggac agtgcttcga tatagaccac gccgaaggtt tcggtgcggc
      301 tggcaaggac gaatgcgtcg ctgttcctca tcaaatccaa gactgcttcg ggctgcaatg
      361 cgcccaaaaa tgtaacggca tgggtaatgc ccaagtctgc cgcctgctgt ttcagccgct
      421 gttcttcctg tccgctgccg ccgatgttca ggcgcagttg cgggcattgt gccaacgccc
      481 gggcaaaggc agtgagtagg acatcgtgtc ctttgagacg gcgaaggtgc gagacggtgc
      541 agaacacgaa atgcggtttg ttgttttttt ctctttgttc aaacggtcgg ttgaatattc
      601 cgcccagtat gttgggggag gtactgccat tcgcagccgt atttgtgttg caggacgtgt
      661 gcgaaatggc ggctgacagc gagcagtgcg gaggcgtgcg ccgctgcttt ttccatcggc
      721 tgccattggt ggggacggat cagaccgcgc gtgatggtgc tgctgtgttc cgtgacgaca
      781 taggggatgc cgtatttttg ggaaattttt tgggcgagta tgccggcata gttcatgcgg
      841 tgggcgtgaa tcaggtcggg cagcccgttt tcgcggatgt agtgtttgaa agctttcaaa
      901 cccgcacaca cccagcggat gcagtcgatg tcgatgaacg gaaagcgggg gaagaaatat
      961 atgccgtgcc atgcatagat gtccaaaccg ctttgccgat attttgcaaa accgtaaggc
     1021 cggtcaggat gcttgctgtt tctttccgca agtagcggaa catcggtgca agcacggcgg
     1081 ttttgatgcc tttcctctgc aatgccagtg cctgattttg aaaaaaatcc cgtccacatc
     1141 ctgttcggat tgcggatacc atgaggggat gacgaggacg tgcaagggtt cgggcatagt
     1201 gggattccgt atcggaaagg cggttattat aagacagacg cagaccgaat atttaaattg
     1261 ttgccttacg ctaatgcaat ttagcgcgcc gatatgctag attggcggtt ttatgagtga
     1321 ggatacggat atgttgcgtt ctattttggc ggcttcccta ttggcggtat cttttccggc
     1381 agcggctgaa gcattgaatt acaatattgt cgaattttcc gaatcggcgg gtgtcgaggt
     1441 ggctcaggat acaatgtccg cacgtttcca agtggcggcg gaaggacggg acaaaaatgc
     1501 cgtcaatgcc gagtttgtta aaaaattcaa cagttttaac agaaaatcga aaaacggcag
     1561 ctttaaaacc gaattggtat cgcgcagtgc gatgccgcgc taccagtaca ccaacggcag
     1621 acgcattcaa acaggctggg aggagcgtgc ggaatttaag gtcgagggta gggattttga
     1681 tgcgttgaac cgttttattg ccgatgttca ggcagatgcc gcgttggaat atacggattt
     1741 ccatgtgtcg cgcgaacgcc gcaacgaggt catcgatcag gtcagcaagg atgccgtttt
     1801 gcgtttcaag gcgcgtgccg aaaagttggc gggcgttttg ggtgcgtccg gttataaaat
     1861 cgtcaaattg aatttgggac acatcggcag ccatatcgcg ggtgggggag ctgctcaggc
     1921 aaaaatgctt cgtgccatgc cgatggcggc aagttacggc gtggagggta cggattccgt
     1981 tgcacctggt gtggaggaaa tcagcatcag cgtcaatggg acggttcagt tctgatttga
     2041 ggtgaacggt aaatgccgtc tgaaacccga cgataagggt tcagacggca ttttgtgttt
     2101 caggctttgg gcagggttac gccggtttgt cccatgtatt tgccgttgcg gtctttgtat
     2161 gaagtttcgc acacttcgtc gctctcgaaa aacaggactt gcgccacgcc ttcgccggcg
     2221 tagattttgg cgggcagggg ggtggtgttg gaaaattcga gggtaacgta gccttcccat
     2281 tccggttcga acggggtaac gttgacgatg atgccgcagc gtgcgtaggt cgatttgccc
     2341 aagcagacgg tcaggacgtt gcgcgggatg cggaaatatt cgaccgtgcg cgccagtgcg
     2401 aaggaattgg gcgggatgat gcagcagtcg tcttcaacgg taacgaagtt ttttgggtcg
     2461 aagtttttgg gatcgacgat ggtgctgttg atgttggtga agattttaaa ttcgtttgcg
     2521 cagcggatgt cgtagccgta gctggacgta ccgtaggaga tgatgcgttt gccgtcggct
     2581 tctttgattt ggttcggctc gaaggggtcg atcatgccga attcttcgct catgcggcgt
     2641 atccatttgt cggacttgat gctcataatg ttttccttgt ttcttgcagt gttcggacaa
     2701 agcattgggg gatgccgtct gaaaacgggg cttatttgtt tttaggcagt ttcacttctt
     2761 taatcatgcc gttttcgcat ttcataatga agagggtttc gccgttttgc gagacggtaa
     2821 atctgccgtg tgccaagcct tttttgaacg tgcccgagag taccatgttg cggaatttgg
     2881 tactgtcgga attgaacggt tcgataaata tttcgcggtt ggcggcaacg gtataaacgc
     2941 cttgtccgtc gaatttgcca tttttaaacg aaccggtata gttgcgcccg tcttggcagc
     3001 gcatgtgcct ttgcctgaag gaagcccgtc ttttccgact ccgccttcgt aggtgcagcc
     3061 gggttcttga taggaagtca gaacggcggc cgaagtgggg agggcgaaca tcatggcggg
     3121 cagtaggaat gcgagatgtt taagcataag ggttattcca ttggattttg gttgacggta
     3181 ttttgtcgtg aaaaagccgt ctgaaaaatc aatcttgcca gccgcccaaa taggaaacca
     3241 gttcttccaa catggtgctg acggattccg ccatcagaat ttgcgaggcg aaggcaaggc
     3301 cggcggcatc gtcgccgttg ctttcggctt cttcctgcaa tacgtcgagg tattggatgc
     3361 gcttgagtgt gaagtcttga gtgaggataa aggcaatttg ttcgcgccac accaagccca
     3421 gttgggtaac ggttttaccg tttttgacgt gttgaaccac ttcgtcggcg gttaaatctt
     3481 gtttggatac tttgacgacg ggaacaatat cgcccgtacc tttgagttcg caatcgctgt
     3541 ctaattcaaa accgccttcg caatgccctt gcaacagcca gccggtcatc aaggaagagg
     3601 gcgattgctt ggtattcggc agcgaggctt ccaaaccgcc caaagcttcg cgcagcttgg
     3661 tcaggatgtt ttctgctttg gcggaagccg cgttattgac gagcaggtag ccgtggcggg
     3721 tgttaaacac cgcttctgta cggctgctgc gggtaaacgc tcggggcagc aggtcgtctg
     3781 taatttgctc tttaagctct tgtttttctt tgcgaccgac attgcgggct tcattgtttt
     3841 ggatttccgc taccttctct tccaaaatat cgcggatgac gccggcaggc aggacttttt
     3901 cttctttttt cagggcgacg cgcaaggtaa agtcggcagg gaaaacgagt tcaggggaga
     3961 atgaaaccgg tgcggtaaag ccttcgctga accagtctaa gccttggcaa tgggtaaatt
     4021 cagcttcagc aagtttgtcg gcaagtacgt ctgcctcagg cagcttttct ttgttgagcg
     4081 gataaaaact aatctgcttg aaccacataa tgtttcctat tgtttgaaat gtcgggaatt
     4141 atttgctgaa ttgttttttc acactgactt tggttttctt cttgaagcgg tttttctctt
     4201 cctttcggcc ttcgcgtttc tcaatacggt tgctcaggct gacgcggcgc agcggttttt
     4261 tcttcggttt gtcttccgca ttttcatacg ggttttccga aacattgtat tgaattctga
     4321 gcggcgtgcc ttgcagattg aaggctttgc ggaacgtttg ggtcagatag cgcgtatagc
     4381 tgtcggaaat cgcgtgcagc gaattgccgt gtaccacaat tacgggaggg ttcatgccgc
     4441 cttggtgggc ataacgcatt ttcggacgca ccaagccggc acgcggcggt tgctgacgct
     4501 cgatcgcgct ttgtaatacg cgcgtgattt tcggcgtcgg catcttaatc atcgccgcgt
     4561 tgtaggcagc ctgaatgctg tcaaacaaac cgtctatacc gcgctctttc aatgcggaaa
     4621 taaagtggaa cttggcaaaa tcgaggaaat acagtttgcg gttgatatcg cgtttcactt
     4681 gctcgcgacg ctcttcgctg atgccgtccc atttgttgac ggcaaccacc aaggcgcggc
     4741 ctgcttccaa agcaaaaccg gcaatcgtcg catcttggtc ggcgatgtcc tgctgcgcgt
     4801 ccaataccaa aacagcgacg tttgccgctt caaccgcctg catcgctttg ataacggaga
     4861 atttttctac tgcttcatcc actttgccgc gacggcgcac gcctgcggta tcgatgatgg
     4921 taaacggttt gccttcgcgt tcgaaatcga tgtggatgct gtcgcgtgtc gtgcctgcca
     4981 tatcgaaagc aatcacgcgc tcttcgccga ggatggcgtt aaccagtgtg gatttgccga
     5041 cgttcggacg gccgatgacg gcaaagacgg gatgttttgc atcggcttct tcggcttccg
     5101 gctctgggaa agtttccaaa atatcttcaa tcagataata cacgccatcg ccgtgcgcac
     5161 ctgaaataac ataagggtcg cccaaagcaa gttcgtagaa ctcggcggca agcacggccc
     5221 tgttgccgcc ttcgcctttg tttaccgcca gataaacagg gcgcgggctt tggcgcaggc
     5281 ggtcggcaat gattttgtct tgcggggtca ggccggtacg gccgtccacc aaaaacacaa
     5341 ccgcatcagc ttcatcgaca gcctgcaagg tttgttttgc catttcgtgc aaaatgccgc
     5401 tgtccacaac cggctcgaaa cctccggtat cgatgaccaa ataaggtttg ctgccgactt
     5461 tgccgtgtcc gtaatggcgg tcgcgcgtca gaccgggcag gtcgtgtacg agcgcgtctt
     5521 tggtgcgcgt caaacggttg aataaagtag atttgccgac gttggggcgg ccgacaagcg
     5581 cgatggttgg tttcatgatt gggtctttct gtgtcaagtg ccgttcggga gaactgaaca
     5641 caagcaggtg tccgttggga cacggcagat ggttttacgg gaattgccgt aggatagtgt
     5701 tgtctgaaat gccgtctgaa gagagggtgg catttcagac ggcatttatt tcagcgaatc
     5761 gagtttcatt tgaaccaatt cacgaccgac agaatcttga ggcatttttt ctaaagcctg
     5821 accgtagttt tttaaggctt cctggctttt tccctgtgcg gcatagacat cgcctttggt
     5881 ttccatcagc aggggggaga agtccgcttc aaccggcgtg tcgagcgcgg caagtgcggc
     5941 atcgtatttt ttttgttgca acaacacaac gccgagacgc tgtgccgcca acgcttgaat
     6001 caggctgtct ttttggttgg acaacaccca tttcaaatgg ccttcagcaa catcgtaacg
     6061 ctgcgcgtca aattcggttg ccgccgccat cagcgtggct tgggcggcgg aaatggaatg
     6121 cgggtagctt tgttggagtt tggccaattc ggcattgatt tcgttttgcg gggctttgtt
     6181 ttgcgccttt tccacgatgt ttgccagcac cgccgccgct tcctgatttt gggaagccgc
     6241 acggttttgg taaaccgtgt atcccaagta gccgagtgcc gccaaaatca gcaaggcaaa
     6301 cagccatttg cccgtggttt tccaaaaata tttaaagttg tctaactctt gttgttcttc
     6361 gagatgggct gccatttatg cgttcttcca ttgttgtaaa gtatgggtta aatcctcggc
     6421 ggcgacagtt tgctgaccgt gtgcgccgtt catgtctttg agcgtaaccg taccgttcgc
     6481 cagttcgtct tgcgcgacaa tcagggcaaa gcgcgcgccg ctgttgtcgg cttttttcat
     6541 ttgtgctttc aggctttgat agccggaatg ctgcattaca ttgaaacctt gcgtgcgcaa
     6601 ggcttgcgcg tatttcatca cctgcaagtc cgccctttcg ccttggtgca ttgcatagac
     6661 atcaggcgca gcgttcactt ccagcgagcc gtattcgctc accaaaagca gcagccgttc
     6721 gatgcccatc gcaaagccga tggacggcgc aggtttgccg ccaagttcct caatcaaacc
     6781 atcgtaacgg ccgccgccgc acacagttgc ctgcgcgccg agtttgtcgg tcgtccactc
     6841 aaaaaccgtc tgattgtaat aatccaaacc gcgaaccaag cgcggatttt caatatattg
     6901 aatacccaaa ccatccaaca tcgccttgaa gcgtgcatag tggttttgcg aagcctcacc
     6961 caagtaatcc accagacgcg gcgcggcgtt gcagatttcc tgcaaatcag ggtttttcgt
     7021 atccaaaacg cgcaaaggat tggttttcag acggcgtttg ctgtcttcgt ccaatttatc
     7081 ttcataacgg gtcagatatt caaccaatgc cgcacggtgt gccgcgcgtt cctcacggtt
     7141 gcccaaactg ttgatttcca aagtcagata ttcgcggata cccaattttt cccataagtc
     7201 ggcagacatc gcgatgattt ccgcatcaat atccggacct tcaaaaccca aagcttcgat
     7261 accgacctga tggaactgac gataacggcc tttttgcgga cgctcgcggc ggaacatcgg
     7321 ccccatatac cacagctttt gcgggctatt gtacagaagg ttgtgttcga ccaccgcacg
     7381 cagacaagaa gccgtacctt caggacgcag gctcaaactc aaagaatcgt ttgaatcgga
     7441 gaaggtgtac atttccttgc cgaccacatc ggtttcttcg ccgatggagc ggacaaacaa
     7501 accggtttgc tcgacaatcg gcgtacggat ttgctgataa ccgtaagcgc gtgtccagcg
     7561 gccgaccgta tcttcaaacg cctgccaaaa cgcagccgtc agtttgaaat ctttttgctc
     7621 gacaggcaga aggtcgttca tgcctttgac ggattggatt ttttgtgcca tttcaagtaa
     7681 gaatgcttaa atcaaattgc gggcgattat agcggatttt taagggcttg tgaggataga
     7741 gatggtttgc ggacgggtaa aaatgccgtc tgaaacagtt ttcagacggc atttgactta
     7801 ctctgcacgt gcttgcctga tttgtccgac tgcaaactct gtctgccgct tgggatgatg
     7861 tgtgaaaaac aatttatttg aaattgtctc ggcttttttc ggcacgaccg ccaaaatctt
     7921 acctgccaaa tttccctcac gggtttgcca agcatccaaa aactgcgccc tgctcattga
     7981 aacatgcccc agcgacgggt cggcaagcaa aaccgtattg ccgtctatcc cgcgcaatac
     8041 cgagaaatga tcatccttgc ggtatttcag atacacgatg acggggattt tcaactgtgc
     8101 gagctgctcg aaagacaggg catagccttt cgcttcaaaa cccaaatctg gcataatgcg
     8161 ccgcatatcc tcaaacgacg cgcgcatctg ctccttatcc agctttttca acacttcttc
     8221 ttccgtcagc gtttgcccgt aaaaattgtt caaaagcgtc gccaccgaag ccgccccaca
     8281 ggaaaaatcc aaatcctgct ttacaatatt gaaatcccgc ctttctttcc aactctgcac
     8341 tttgattttt ccgtaaacaa caggattatc gttaaacatc ggtgcagcat tcaaacgata
     8401 agataaagaa acgacaacac acgccaacag aaaaacatat ttgaacttca tcatattgtc
     8461 cacataaagg gcagcctgaa aatctttcag gctgcccttg tcaaattatt cctagctttc
     8521 ggcttttttg gcaaaccaaa caatccgatt acccgcataa tactttccat ttattgaaat
     8581 ccgacaagcc gcgcccaaaa aatgccatgc actgtcgatt tccgcagcaa tctttgtacc
     8641 gttttcttca aattccaaat attcacccaa taataaactt gaaacagaac gcgtgggcag
     8701 caagtgccca ccctacgctt attcaaataa tttgattaaa taaagaataa agaaaatgca
     8761 tcaggaacaa aattataatc tgccacctga ctcacaccgc ttttaaagta aggggcatca
     8821 aaatcaaaac cgcaaaaaaa ataatttttg cattgatttt taatagattt aaaattcgaa
     8881 tatagtgttt ctccagattt aaaaagttta tctttatcta tatatttgat gctttcccta
     8941 tccaaaataa atatttcaaa cattaaaaaa tcattacatg accaagccaa atcataaatt
     9001 tgattcaaat gggtatcata aagataaaaa taataaggtt tgggaacagg taaaatattt
     9061 aatggaaaag gaggactaat tttcttaata tctgatgact gatatccata tctttctata
     9121 ttcttaaaaa tttcatcttt cttaagataa caagacattc tgacaatcac aacctgttca
     9181 tcggatatat tatctatttg catggcgcgt ataacacgcc atgcctgatt aaaattagtc
     9241 tcccttacct tagaatctat tatctttttc aaagatgaaa atgcccctta tcttgaactc
     9301 tccagttaga acctggaagt ttcgtacctc ttaaatgtgt attaatattt cttatagttt
     9361 cattaaaatg ccacgcgctg ccgatttcaa cggtaatttt cgtaccgctt tcttcaaatt
     9421 ccaggtattc ccccattagc taacgcaaag aagcagacgc catttcggct tcgttatgat
     9481 aaacccgcct tccgttgata tagacttccg cccctgtccg ctccaaattc caaaaattcc
     9541 gtactgaaat ttccatatcc cgatattgtg cagaccatgt tttttcgaag gttttcataa
     9601 aatttcctat acctgtccaa tcggcacata tcaattgcat ta
//
LOCUS       DAWFBO010000072         9512 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFBO010000072 DAWFBO010000000
VERSION     DAWFBO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.072"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            134..343
                     /locus_tag="WGO87_001227"
     CDS             134..343
                     /locus_tag="WGO87_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0392584.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            432..1646
                     /locus_tag="WGO87_001228"
     CDS             432..1646
                     /locus_tag="WGO87_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0392585.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1733..4312)
                     /gene="clpB"
                     /locus_tag="WGO87_001229"
     CDS             complement(1733..4312)
                     /gene="clpB"
                     /locus_tag="WGO87_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0392586.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLSSLPKVSGQGGDIMPSRELQAVLNLMDKAATKRG
                     DVYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4572..5582
                     /gene="trpS"
                     /locus_tag="WGO87_001230"
     CDS             4572..5582
                     /gene="trpS"
                     /locus_tag="WGO87_001230"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0392587.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANSENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAAKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5650..6195
                     /locus_tag="WGO87_001231"
     CDS             5650..6195
                     /locus_tag="WGO87_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392588.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENTAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLDLGQSSEGKTNDGKKQISYPIKGLPEQNVVRLTGKHTNDLEAVVGKCME
                     TDGKGAPSGWAANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            6514..6837
                     /locus_tag="WGO87_001232"
     CDS             6514..6837
                     /locus_tag="WGO87_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392589.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(7046..8554)
                     /gene="ppx"
                     /locus_tag="WGO87_001233"
     CDS             complement(7046..8554)
                     /gene="ppx"
                     /locus_tag="WGO87_001233"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0392590.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALSERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(8671..9402)
                     /locus_tag="WGO87_001234"
     CDS             complement(8671..9402)
                     /locus_tag="WGO87_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0392591.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLRLSLGYKNR
                     SVTRSFGIVPFIGQNLLGGSRYNFVGGFNADFSRRLSERWRLTLNVGNMWKRYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTLVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNMQIFMSVEKDFK"
BASE COUNT         2213 a         2473 c         2492 g         2334 t
ORIGIN      
        1 tgcggcttcg tcgccttgtc ctgatttttg ttaatccgct atatcgattt gaatttttca
       61 gaaaatgaag cggacgtttg ggcggaggca acttgtttga taagatagca atatttcaaa
      121 acggagaaag atcatgcctt atgtcaatat taaagtaacc ggcggcaagg aagcaccgac
      181 tgccgcgcaa aaagcggaac tgatcggcgg cgtaaccgaa ttgctggcac gcgtgctggg
      241 caaaaatccc gaaacaacgg ttgtcgtgat tgatgaagtg gataccgata actggggaat
      301 aggcggcaaa agcgtcagcg aacggcgcaa agagggcagg taaaaagcct gaaaatctcg
      361 gtttgatgct ttaaattccg cgtgaaaaag agtacattcc cacccattgc ccaaaattta
      421 cggaacacat catggataaa tttcccaagt ctgcaaagct cgatcacgtc tgttacgaca
      481 tacgcgggcc ggttcacaaa aaagcccttc agttggaaga ggagggcaat aaaatcctta
      541 aactcaatat cggcaaccct gcgccgttcg gctttgaagc ccctgatgaa atcttggtcg
      601 atgtcatccg caacctgccg acttcgcaag gctattgcga ttctaaaggg ctgtattccg
      661 cccgcaaagc cattgttcac tactatcaga ccaaaggttt gcgcgatatt acggttgatg
      721 atgtctatat cggcaacggc gtgtccgagc tgattacgat gtctatgcag gcattgctca
      781 acgacggcga cgaaatcctg attcccgcgc ccgactaccc tttgtggacg gcggcggcaa
      841 cgcttgcggg cggtacggta cgccattatc tgtgcgacga agaaaacggc tggttcccca
      901 accttgccga tatggaagcc aaaatcacgc ccaaaaccaa agccatcgtc gtcatcaatc
      961 ccaataatcc gacaggcgcg gtgtacagca gggaaatcct gttggaaatc gccgaactgg
     1021 cgcgcaagca cggtttgatt attttcgccg acgagattta cgataaaatc ctttatgacg
     1081 gcgcggttca ccaccacatc gccgcgcttg cccccgacct tttgacggta acgttcaacg
     1141 gtttgtccaa atcctaccgc gtcgccggct tccgccaagg ctggatggtg ctcaacgggc
     1201 cgaaacatca cgcaaaaggt tacatcgagg gtttggatat gctctcgtct atgcgcctgt
     1261 gtgccaatac gccgatgcag cacgcgattc agacggcatt gggcggctat cagagcatca
     1321 acgaatttat tttgcccggc gggcggcttt tggaacagcg caacagggcg tgggaactgg
     1381 tcagccaaat ccccggcgtg tcctgcgtca aaccgatggg cgcgatgtat atgttcccaa
     1441 aaatcgatac cgaaatgtac cgtatccacg atgacatgaa attcgtttac gatttgctgg
     1501 tgcgcgaaaa agtcttgctg gtacagggaa cggggtttaa ttggattaaa cctgaccatt
     1561 tccgcattgt cacgctgcct tacgtccatc agattgaaga ggcgatgggc aggttggcaa
     1621 gattcctgca aacctaccgc caataagggg acggtttgtc tgccgaggat aaaaaatgcc
     1681 gtctgaaacg gagattcccg tttcagacgg cattttcaac agcaggaacg aatcaggcaa
     1741 atttcagtct gtcgccgtcg gcttctaccc tgatttcgct ttcgggcgca tagtttccgg
     1801 caagcagggc ttttgccagc gggttttcga tttccgactg gatggcgcgt ttgagcggac
     1861 gtgcgccgta aatcgggtcg aaaccggctt tggcgatgat gtccagtgcg gcatcggaaa
     1921 cagtcaggcg caggttttgt ttttccaaac gtttttccaa acctttgagc tggattttcg
     1981 caatgctgcg gatgtttgcc tgatccaaac cgtggaacac gaccacttcg tcgatgcggt
     2041 tgatcatttc aggacggaaa tgttctttca catcctccat cacaacttct tttaccgctt
     2101 cgtaatcctg aatgcccatt tgttggatgt gctggctgcc gatgttggaa gtcatcacaa
     2161 tcacggtatt tttaaaatct accgtcctgc cttgcccgtc ggtcaaacga ccgtcatcca
     2221 atacttgcag caggatgttg aacacatcgg gatgggcttt ttccacttcg tccagcagaa
     2281 tcacgctgta cggtttgcgg cgcacttgtt cggtcaggta gccgccttct tcgtagccga
     2341 catagcccgg aggcgcaccg atcaggcgtg caacggcgtg tttttccata tattcggaca
     2401 tatcgatgcg aatcagatga tcttcgctgt cgaacagaaa gcctgccagg gctttacaca
     2461 actcggtttt acccacgccg gtcgggccca agaacaggaa gctgccgtaa ggcttgttcg
     2521 gatcggcaag accggagcgg ctgcggcgga tagcgtcgga cacggcacgc acggcttcgt
     2581 cctgtccgac cacgcggcgg tgcaatactt cttccatttt cagcagtttg tcgcgttcgc
     2641 cttccatcat tttggatacg ggaatgccgg tcatacggga aaccacctct gcgatttcct
     2701 ctgcgccgac attattacgc aagagtttgt ttgccggttt tgtgctgtcc gtatctgccc
     2761 gttcggcggc tgcacgctgt ttttccaaat gctccaaatc ttcatacatc aattttgaag
     2821 ccagtgccaa atcgccttgc cgttttgcct gttcgatttt aattttgact tcgtcaattt
     2881 gtttcttaat attagcagca ccgtctgaaa ttgctttttc ggctttccag atttcgtcta
     2941 aatcggcgta ttctttttgc agaccgttga tttcctcgtc tatcagttcc aaacgttttt
     3001 tgctggcatc gtctttttct ttttcaacgt gcgccttttc catccgaagc tgaattagac
     3061 ggcggtcgat tttgtccatt gcttccggct tggtttcttt ttccatcttg acacggctgg
     3121 cggcttcgtc aatcaaatca atcgctttat cgggcaggaa gcggtcggtg atgtagcggt
     3181 cgctcaactc cgctgcggca acgatagcag ggtcggtaat atcgataccg tggtggattt
     3241 cataacgctc ctgcaaaccg cgcaagatgg cgatggtgtc ttccacgctg ggctcgccaa
     3301 ccaatacttt ttggaaacgg cgttcgagtg ccgcgtcttt ttcgatgtat tggcggtatt
     3361 cgtccaaggt ggtcgcaccg atacagtgca gttcgccgcg tgccaaagcc ggtttcaaca
     3421 tattgcccgc gtccatcgcg ccgtcggttt tacctgcgcc aaccaaggta tgaatttcgt
     3481 cgatgaaaat cagagtgttg ccatcgtctt tcgccaagtc gttcaacacg cctttcaagc
     3541 gttcttcaaa ttcgccgcga tatttcgcgc cggcaatcaa agccgccaag tccagaacca
     3601 gcaggcgttt gttgcgcagg gattcaggca cttcgccgtt gacgatacgt tgcgccaagc
     3661 cttcaacaat agcggtttta cccacacccg gctcaccaat cagcacgggg ttgtttttgg
     3721 tacggcgttg caatacctga atcgcgcggc ggatttcatc atcacggccg ataacggggt
     3781 caagtttgcc gtcgcgggcg cgctgggtca gatcaagtgt gtattttttc aaagcatcgc
     3841 gttggtcttc ggcattcgca tcattcacgt tttgtcctcc tcgtaccgca tcaatcgcgg
     3901 cattaatgtt ttgttcggtc gcaccggctt ctttcaaaat tttgccggtt gcatcgttct
     3961 gctgcaccaa ggcaagcagg aaaagttcgc tggcgatata gacatcgccg cgcttggttg
     4021 cggctttgtc catcaagttc aatacagctt gcagctcacg gctaggcata atatcgccgc
     4081 cctggccgga cactttcggc aggctgctca aatgctgctg caaacgctgt ttcacctgcg
     4141 gcacgttcac gcccgaatgc gccagcaacg cggcggcacc gctgttttgg tcgtcaagca
     4201 gggcttttaa cacaaagcct gcttccagat agctgccgtc cgcagccaac gccaaactct
     4261 gagcttctgc aagggcttgt tggaatttgg cggttaattt gtcgtatcgc atttttgttt
     4321 ccttttcaaa atgtccgctg tcgaagccta tatgtgcata attgtggata actcaagttc
     4381 tgttttctgt ttttctatat ttaattcgat atatcattga atttaaagta tataaaaatg
     4441 tataataatg tgtataacta tatcttctta atatggaaaa gtctgttgtc ggctggatgt
     4501 aggtggcaaa tcgggtataa tcggcacatc tttttccctt tcagacggca ttgatgccgc
     4561 aaggacattt tatgagcaaa aaacgagttc tgaccggcgt aaccaccacc ggcatcccgc
     4621 atctgggcaa ctacgtcggt gctatccgcc ccgccgtccg cgcggcgcaa aaccctgata
     4681 ccgaatcctt cctcttcctc gccgattacc acggtatcat caaatgccac gagccggaga
     4741 tgattcacca atccacccaa gccgttgccg ccacttggct tgcctgcgga ctcgaccccg
     4801 agcgcaccac cttctaccgc caaagcgaca ttcccgaagt gatggaattg aactggattc
     4861 tgacctgcat caccgccaag ggtttgatga accgagccca cgcctacaaa gccgccgtgc
     4921 aggcaaattc agaaaacaat caggaagatc ccgatttcgg cgtggaaatg ggtttgttca
     4981 gctatccgat tctgatgacc gccgacatcc ttatgttcaa cgccaacgaa gtgcccgtcg
     5041 ggcgcgacca aatccaacac gtcgaaatgg cgcgcgacat cgccggccgc ttcaaccacc
     5101 gctttaaaga aatctttacc ctgcccgaag tgaaaatcga tgaaaacgtc gaactcttgg
     5161 tcggtttgga cggacgcaaa atgtccaaat cctacggcaa caccattccg ctttgggaaa
     5221 acgacaaaaa aacccaaaaa tcggtcaaca aaatcatcac caatatgaaa gagccgggcg
     5281 agccgaaaca gcccgacgaa agcccattgt ttgaaatcta caaagccttc tccacgccgt
     5341 ctgaaaccgc agcgttcacg caaatgcttg ccgaaggctt ggcttggggc gaggcaaaaa
     5401 aacttttggc ggcgaaaatc aacgccgaac tcgccgaacc gcgcgaacgc tacaacgagc
     5461 tgaccgccga cccttcgcaa atcgaagaga ttttgcaggc aggcgcggcg aaagcgcgta
     5521 aagaagcacg cgaattgttg gacaaagtac gcgatgcggt cggcatccgc ccgttgaaat
     5581 aaatccatgc cgtctgaaca tccattgtcc gtgtttcaga cggcattttg aaaccatcag
     5641 gagtgtggaa tgctgaaaac atcttttgcc gtattgggcg gctgcctgct gcttgccgcc
     5701 tgcggcaaat ccgaaaatac ggcggaacag ccgcaaaacg cggtacaaag cgcgccgaaa
     5761 ccggttttca aagtcaaata tatcgacaat acggcgattg ccggtttgga tttgggacaa
     5821 agcagcgaag gcaaaaccaa cgacggcaaa aaacaaatca gttatccgat taaaggcttg
     5881 ccggaacaaa acgtcgtccg gctgaccggc aaacatacca acgacttgga agccgtcgtc
     5941 ggcaaatgta tggaaaccga cggaaagggc gcgccttcgg gctgggcggc aaacggcgtg
     6001 tgccatacct tgtttgccaa actggtgggc aatatcgccg aagacggcgg caaactgacg
     6061 gattacctga tttcgcattc cgccctgcaa ccctatcagg caggcaaaag cggctatgcc
     6121 gccgtgcaga acggacgcta tgtgctggaa atcgacagcg aaggggcgtt ttatttccgc
     6181 cgccgccatt attgaggtat tcggacatcc cggaatatat ttgggttttt caaaccctgc
     6241 aggaaaaagt ccgcaccgtc gggaaactca aaaggagggg gatatgtgtt acaattttcc
     6301 gaactgtttt cataaaatag ttttcggacg tgtttcaata tggcattgat gccgccttat
     6361 tgttcgagaa aaaaccttta tatttaaata taatgggttt taactaaacg ggaaaccgtt
     6421 ttctctccgg ccgatgggca aaatcagccg attgatggaa cacggtccga tttttaagca
     6481 aaaccttaat caacaactca agaaggataa gacatgaaaa aattattgat tgccgcaatg
     6541 atggcggctg ccttggcagc ttgttcgcaa gaagccaaac aggaggttaa ggaagcggtt
     6601 caagccgttg agtccgatgt taaagacact gcggcttctg ccgccgagtc tgccgcttct
     6661 gccgtcgaag aagcgaaaga ccaagtcaaa gatgctgcgg ctgatgcaaa ggcaagtgcc
     6721 gaggaagctg taactgaagc caaagaagct gtaactgaag cagctaaaga tactttgaac
     6781 aaagctgccg acgcgactca ggaagcggca gacaaaatga aagatgccgc caaataattt
     6841 gttgccttgg caaaaatgat gggatgccgc ctgccggcaa cccaaacgaa accgcctgaa
     6901 gattttcagg cggtttttga gttgtatgcc ccttgttttt tacgcctgca tgaccgttcc
     6961 gagcagatgt gccgcgcata tcgggatgtt gcggacaggc aaatgccgtc tggacagggt
     7021 ttggacggca tttgttccgt aaggttcaga cgacctcgac tttaaacggc atattgattt
     7081 tttgccattg gacgctttca tattccaatg cgtcggcaat caggggatgg cgttccagcc
     7141 attccctgtc aatacgcagg atgaagccgc agctttccgt atccgtgcgc aactgcatat
     7201 ttttcgggaa agacaggtct tggcgcgaac ggcagaacag ggcagcaagg cgcagggaca
     7261 aaacggcata ccacaacatt tcgttggtgc cgatgatgcc gctcattttt ttcatatcgc
     7321 cgcgatgacc gatgaccagt tgggcaagta tggtctgttc tttgcgtgag aaacccggca
     7381 tatcggcgtt ttcgaggatg taggcggaat gcttgtgata gccggtgtgg gcgatgtcca
     7441 aaccgatttc gtgcagcgcg gcggcgcgtc cgagatactg ttgccacaag gcaagctctt
     7501 gaactgtaac gtttttagcg tggcagaggc tgtccataaa ggtttgcgcg gtctcggcgg
     7561 tgcgtttcgc ctgattgagg ctgacgtggt agcggtgttg gaactcggca accgtttgtc
     7621 cgcgcatatc ttcgtttaaa ccgcgcccga tcaaatcgta aaacacgccg tcgcgcaggg
     7681 cggcttcggt tacggtcatc ctgtcgagtt tcatttcctc aaacgccgcc atcatcacgg
     7741 caagtccgcc ggcaaaaact tcgatgcgtt ccggtttcag gttttcaaat ttggcttttt
     7801 tgaccgaacc ggcttcgatg atgcgttcgg agagggcgcg catacctttg taggtaatgt
     7861 ccgcctcttg gggcatttcg gcggcaagca cgtcgcggat ggattttgcc gaacccgatg
     7921 tgccgacggc gaaatcccag ccttcgcgcc tcatattttt gctgatgcgc tggatttcgt
     7981 tgcgggcggc ggaaatggcg gcttggaagt ctttggcggt gattttgttt tggaagaagc
     8041 gcaggctgta ggtaacgcag cccaagggca ggctttcggt aatgtcggga ttcagcgtcg
     8101 aaccgatgac aaattctgtc gaaccgcccc cgatgtcgat aaccagcatt ttgccgccgc
     8161 ccggggggag ggtgtggatc acgccggtat aaatcagccg cgcctcttcg cgcccggcga
     8221 tgatttcgat ggggaaaccc agtgccgctt cggctttggg aaggaaatct gcgatgtttt
     8281 tggcgacacg gaatgtattg gttgccacgg cgcgtacctg ttcagggcgg aagccgcgca
     8341 ggcgttcgcc gaattttgcc agacagtcca aagcctgttc ttgggaagcg gcactcaggt
     8401 ttttctgttc gtccagaccg gcggcgaagc gcaccatctg tttgaacgaa tcgataactt
     8461 tcaactgtcc gttgttgttt tcgcaaatct ggaggcggaa actgttggaa cccaaatcga
     8521 cggaggcgag gatgtttgcg ggggtggtgg tcatggcgga tatcggtggg ggaaaaacgc
     8581 aatgttactc tgacggcgca ggcgttgaca ataaatgatg cggctgtttt tgattctgct
     8641 cgcggatgtt gccgacggca ttttttgcgc ttatttgaaa tccttttcca cgctcatgaa
     8701 aatctgcatg tttttgcgtg tgtaaaaact tttcatattg ctgtcgattt tcagatagcg
     8761 gaaattgagt tgcggcgtaa agcccttcca agagattttg tcgtgccaca acgacaggtt
     8821 tgcctgatat tcgtggtctt tgcgcgggaa gcggtacaca agggtcccgg gtgcatcaaa
     8881 agtcctgcgg gtgtagcgca ggtttgcccg cagacccaag ccgccgtcga acgttttgac
     8941 cgctccgaca cgcaaaccct tgcggatgga agcctgttcc gcctccttcg tcaagtcgtg
     9001 cgaccagtcc gcaccgccgt aaagcagcca gtctttcggc gcggaataca tcagcgtcgc
     9061 gcccgccagc ggcatatggc tgtcgtatcg ggcggcggtg cggtcttcct gataacgctt
     9121 ccacatattg cccacgttca gcgtcagccg ccagcgttcg ctcaagcgtc gggagaaatc
     9181 ggcattgaag ccgccgacga aattgtatcg gctgccgcct aagaggtttt gcccgataaa
     9241 cggcacgatg ccgaacgagc gcgttaccga acgattttta tagccgagcg acaggcgcag
     9301 gcactgttcg ctgaaatctt tgttatccca ataatgcacg ccgccgccgc tgatgccgcc
     9361 gtagaggaaa tgatgcccgc ccgcattgat ttcgcgcgac acgcctaagc cctagcgcaa
     9421 accgtgtgcc ttttgcggca ggctgtcggc ggttttcgtc cagcttctgc ccgcaaattc
     9481 aatcgttttt ttggacgaag cgttgtttat ag
//
LOCUS       DAWFBO010000073         9421 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFBO010000073 DAWFBO010000000
VERSION     DAWFBO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.073"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(115..1557)
                     /gene="aldA"
                     /locus_tag="WGO87_001235"
     CDS             complement(115..1557)
                     /gene="aldA"
                     /locus_tag="WGO87_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0392592.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVST
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1592..1792)
                     /locus_tag="WGO87_001236"
     CDS             complement(1592..1792)
                     /locus_tag="WGO87_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392593.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(1801..2190)
                     /locus_tag="WGO87_001237"
     CDS             complement(1801..2190)
                     /locus_tag="WGO87_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392594.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2398..3315)
                     /locus_tag="WGO87_001238"
     CDS             complement(2398..3315)
                     /locus_tag="WGO87_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0392595.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFAGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITTSEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3488..4288
                     /locus_tag="WGO87_001239"
     CDS             3488..4288
                     /locus_tag="WGO87_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0392596.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4336..5112
                     /gene="mlaE"
                     /locus_tag="WGO87_001240"
     CDS             4336..5112
                     /gene="mlaE"
                     /locus_tag="WGO87_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0392597.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5163..5657
                     /gene="mlaD"
                     /locus_tag="WGO87_001241"
     CDS             5163..5657
                     /gene="mlaD"
                     /locus_tag="WGO87_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0392598.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGNSGKTYTVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5694..6284
                     /locus_tag="WGO87_001242"
     CDS             5694..6284
                     /locus_tag="WGO87_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0392599.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6341..6619
                     /locus_tag="WGO87_001243"
     CDS             6341..6619
                     /locus_tag="WGO87_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0392600.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6616..7443
                     /locus_tag="WGO87_001244"
     CDS             6616..7443
                     /locus_tag="WGO87_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0392601.1"
                     /translation="MKKTAYAALLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7568..7930
                     /locus_tag="WGO87_001245"
     CDS             7568..7930
                     /locus_tag="WGO87_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0392602.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7927..8313
                     /locus_tag="WGO87_001246"
     CDS             7927..8313
                     /locus_tag="WGO87_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0392603.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8310..8798
                     /locus_tag="WGO87_001247"
     CDS             8310..8798
                     /locus_tag="WGO87_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0392604.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKMANYYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8881..9048)
                     /locus_tag="WGO87_001248"
     CDS             complement(8881..9048)
                     /locus_tag="WGO87_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0392605.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9015..9395)
                     /locus_tag="WGO87_001249"
     CDS             complement(9015..9395)
                     /locus_tag="WGO87_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0392606.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
BASE COUNT         2178 a         2624 c         2372 g         2247 t
ORIGIN      
        1 aaagacaaaa acatcgatgt cggtcatgac tgattgccgg tgaaactgta aaaatgccgt
       61 ctgaacctga aaacgggttc agacggcatt ttctatcggg gttttaaagc ggcattaaat
      121 gtcggtttcc aaataaacga cttgggtttg cagatattct tccaaaccgt gtttgccgtc
      181 cgcgccgccg ataccggatt ttttccaacc ggcgtggaaa ccctgcatcg cttcaaagtt
      241 ttcgcggttg atgtaggttt cgccgaattg caggcggcgg gtaacgtaga aggcttcgtt
      301 taaattagtc gtataaacag aactggtcag accaaactcg caatcgttag ccagtgcgat
      361 gacttggtcg agcgtgtcga aagtggaaac aggcaggacg gggccgaagg tttcttcttt
      421 cataatgtcc atactgttgt cggtgtcggt cagcagggtc ggctcgaaga aatagccgcg
      481 tccttcggcg cgtttgccgc cgcaaaccaa tttcgcacct tgtttgactg cccgttccac
      541 tttttcggca acggctttga cggcgcgctc ttcaatcagc gggcccattt ccagcgcgcc
      601 ggcttcggct tcggcagggt tgccgtagcg cacgcctttc atcgcggcgg tcattttttc
      661 gataaacgcg tctttcaggc tgctgtggac atagacgcgc tcggcgcagt tgcagatttg
      721 accagtgttg ccgacgcgcg aagccaagat ggatttcacc gccaaatcca aatccgcgtc
      781 tttcaaaacg atggcggggg ctttgccgcc gagttccagc gaaactttgg tgatgttggc
      841 ggaagcggct tccatcactt ggcggcctgc ttcgacggag ccggtcaggc tgaccatatc
      901 gacttgcgga tgggcagaca aggcattgcc gatttccgca cccggaccgt tcaccacgtt
      961 gaacacgcct gcgggcaatc cgaccgcatc gacgatttcg gcgaagatgt ggcagttgat
     1021 cggggttacg ctgctgggtt tgacaacgat ggtgttgccc gttactaagg cgggacccat
     1081 tttgcgggca atcaggaaga aagggaagtt ccacggcaaa atgccggcaa ttacgcccag
     1141 cggacgtttg aacaataaaa tattttcacg cgggcggtcg ctttggatga tttcgccttc
     1201 gtaacggcgc gcccattcgg cttggtaatc gagatagtcg gcggtgaaca tcacttctac
     1261 gcgcgccaag tctttggttt tgccgccttc ggcaacgatg gtgtcggtca gctcgtcggc
     1321 gcgtccgcgt atgccttggg cgattttgcg caaatacgcg ccgcgttcga ccgcaggcag
     1381 gcgttcccaa gccggttgcg ccgcgcgtgc cgccgccaca gctcggtcaa cgtctgcttt
     1441 tccgcctttg ggttcgcggg cgatggtttc ttcggtggaa gggttcaata cgtcgcgcca
     1501 ttcgccgttg aagtcgtttt caaagcgtcc gttgatgtac atggccaatt gtttcatttc
     1561 gggttctcca gttttgtagt cagatgtagt tttagtttat tcccaaataa attaccgtac
     1621 aagtttcttt tgaaatgttt agacgagaag ctactatgtc tgcatattgt tctgctaatt
     1681 tatattcact cgaatgcaac tgccgattat tccaatctgc attcgcatca gtaaaagccc
     1741 cagcattgcc gcctgtggca taaccaatcg ctaaactgcc aacggtatcc acaacaggtt
     1801 tcaggctgcc tgaatttctc taataaatga aacacttatt ctatcagccg ttaatgtaac
     1861 caaacaatta tcaagataag agtagtttat taaatactca tcttcttcaa attgtatatt
     1921 ttgtagggtt agatgattat tttttaattc tgccgttatt tcatacttga aactatcatc
     1981 tatttttcta aatccaatat ttgaagttga tttatccagt atgtaatcat taaagatttc
     2041 ataggataat tccacttcat aaaattgatt ttcaaatatt ttgaatgggc agtacgatat
     2101 aaaacaatgt agttcataac catttatttc aatcaatact gcctcttcaa tggtgttatc
     2161 taactttttg attttacatt tatataacat ttatcttgcc tttggataaa ttgttttgtt
     2221 ttttggattg ttattcaaag ttctccaaaa gtcttttcct gattttgtat gtatcgtggt
     2281 aatactacca ttttcattgg ttccaacaaa aataaattta gattgacctt tggaatcagt
     2341 acccaaaaac tgaacatatc cagacacgcg gatttcgggt tttaaatcaa atgcggctta
     2401 ccggttttct tttttggttt ccgcaggcgt gctgccgaaa tattgcctga atgcctgagt
     2461 aaagctggaa acgtggcggt agccgcataa atatgcggtt tcgcctatgc ttttgccccc
     2521 gttttgcaac agataaagcg cgtgctgcat ttgtttgtgg tgcagccact cgcttgtggt
     2581 aatgccgaaa tggtcgcgca tacggcgttg cagcgtcctt tcgctgatgt tcagcgcgtc
     2641 tgtcagccgg ttgacttggt gtgcgccgcc ggcaaacgcg gcattcaggg tgcggctgaa
     2701 gtcttcagac ggcatagcgt ctgcttccgc cgtttgcccc gccgccggcc cgatgccgtc
     2761 tgaaaccgtg tcccacaagt ccgacagcag ccgcaacacg tccgcctcgc ggcgcaatgt
     2821 ttcgcccaaa tgcccctttg ggacggcttt caggcaggat gccgccaagc cgcgcaggtt
     2881 cgggggcaaa tcccatatcc tgaccggttc gcggtaaagc aggggtgcga aacgcgcgta
     2941 ttccggacgc agcagccatt gttccatacc tttaatggtc attttgaccg ttttgccgcc
     3001 tcggtaaaga tagcggctga acaggacttc ttccccgaca gcaattagga caatcttgcc
     3061 gccgtccgca tcgatttgga agcggcagcg gttgatgccg aagtccaaac tgccttccag
     3121 caagagcacg aacgacacat aaggttcggc gaggcggctg ctgcaaaaat cacaccgtgc
     3181 cgttactgtg ccgccgtgga tggaaatgcc gttggacaaa gtatcgaaac ggtagctgcc
     3241 ctccacatag ctgccgccgc cgcttttgac tagattgacg aaccggctgc tgttcaaatg
     3301 ttctgctttt tccatagttt tctgataaag gttatcattt gaaagataac atttttcgcc
     3361 acagcaaaca atccgccgcc gacaacggca ttttcacaac atttaccgtc cgtatgccgt
     3421 ctgaaaattg cccgtcttcc cgataagcct taaaatacac gccgtcaaac ctaccgtccg
     3481 tccgtacatg agcccatccc cctttatcga aatgaaagac gtcgccttcg cgtatggcga
     3541 ccgcccgatt ctgaagaaca tcaatttcag cattccgcaa ggcaattttg ctgccgtaat
     3601 gggcggttcg ggcagcggca aaaccacgct gatgcgactg attacaggac agattcgtcc
     3661 gcagtccggg caggttttga ttgaaggacg ggatttggcg ggcttttcgg ctgacgagtt
     3721 gtatgaacac cgccgccgta tgggcgtatt gttccaacac ggcgcgctgt ttactgattt
     3781 gtcggtattc gacaatatcg cttttccgat gcgcgaactg acgcgcctgc cggaagcggt
     3841 gattcgggat ttggttttgt tgaaattgaa cgcggtcggt ctgcgcggtg tggaaaacct
     3901 gatgccgtcc gagttgtccg gcgggatgtc gcgccgcgtc gcgcttgccc gcacgattgc
     3961 gctcgaccct gaaattatgt tgtacgacga gccgtttacc ggcctcgatc cgatttcctt
     4021 gggcgtgatt gcccacttga tcagccgcgt caacaaggct ttgcgttcga ccagtattat
     4081 ggtaacgcac gacattgaaa aatctttgga aatcgtcgat caggtgattt tcttggcgca
     4141 cggcgaaatt atgttctccg gctcgccgca ggaaatgcgc gaactggatt cgccttgggt
     4201 gcgccagttt gtcggcgggc tggcagacgg ccccgtagca taccgttatc cggcgcaaac
     4261 gtcgttgcag caggacctgt tgggataaat gagttgatgc cgtctgaaac cgttgaactt
     4321 tacacataga aatttatgaa ctttatccgt tccgtcgggg cgaaaaccct cggccttatt
     4381 caatccctcg gcagtatcac gctgtttctg ctgaacattc tggcgaagtc cggtacggct
     4441 ttcgtccgtc cgcgcctgag cgtgcgccaa gtgtatttcg ccggtgtgct gtcggtgttg
     4501 attgttgccg tttcggggct gtttgtcggc atggtcttgg gtttacaggg ctatacgcag
     4561 ttgtcgaaat tcaaatccgc cgatattttg ggctatatgg tcgcggcttc gctattgcgc
     4621 gagctgggtc cggtattggc agcgattttg tttgccagca gcgcgggcgg tgcgatgacc
     4681 agcgaaatcg gtctgatgaa aacgaccgaa cagctcgaag cgatgaatgt gatggcggta
     4741 aaccctgtcg cccgcgtggt tgcgccgcgc ttttgggcgg gcgtgttttc catgccgctt
     4801 ttggcttcga ttttcaacgt ggcgggcatt ttcggtgcgt atttggtcgg tgtaagctgg
     4861 ctgggcttgg acagcggcat tttctggtcg caaatgcaga acaacatcac gatacattac
     4921 gatgtaatca acggtctgat caaatccgcc gcgttcggcg tggcggtaac gctgattgcc
     4981 gtgcatcagg gcttccactg cgtcccgacc tcggaaggca ttttgcgcgc cagcacgcgc
     5041 acggtggttt cgtccgccct gacgattttg gcggtcgatt ttatattgac cgcgtggatg
     5101 tttacagatt gacagggcgt ctgaagacga aacgcggaac atcggatatt caaggaactt
     5161 taatgaaaaa gaacatattg gaattttggg tcggactgtt cgtcctgatt ggcgcggcgg
     5221 cggttgcctt tctcgctttc cgcgtggcgg gcggcgcggc gttcggcaat tccggcaaga
     5281 cttataccgt atatgccgat ttcggcgaca tcggcggttt gaaggtcaat gcccccgtca
     5341 aatccgcagg cgtattggtc gggcgcgtcg gcgctatcgg acttgacccg aaatcctatc
     5401 aggcgagggt gcgcctcgat ttggacggca agtatcagtt cagcagcgac gtttccgcgc
     5461 aaatcctgac ttcgggactt ttgggcgagc agtacatcgg gctgcagcag ggcggcgaca
     5521 cggaaaacct tgctgccggc gacaccatct ccgtaaccag ttctgcaatg gttttggaaa
     5581 accttatcgg caaattcatg acgagttttg ccgagaaaaa tgccgacggc ggcaatgcgg
     5641 aaaaagccgc cgaataaacg tatgccatca tattcaaact aggacactta atcatgaaaa
     5701 aatcctccct catcagcgca ttgggcatcg gtattttgag catcggcatg gcatttgccg
     5761 cccctgccga cgcggtaaac caaatccgtc aaaacgccac tcaagtattg agcatcttaa
     5821 aaagcggtga tgccaacacc gcccgccaaa aagccgaagc ctatgcgatt ccctatttcg
     5881 atttccaacg tatgaccgca ttggcggtcg gcaacccttg gcgcaccgcg tccgacgcgc
     5941 aaaaacaagc gttggccaaa gaatttcaaa ccctgctgat ccgcacctat tccggcacga
     6001 tgctgaaatt aaaaaacgcc aacgtcaacg tcaaagacaa tcccatcgtc aataaaggcg
     6061 gcaaagaaat catcgtccgc gccgaagtcg gcgtacctgg gcaaaaaccc gtcaacatgg
     6121 acttcaccac ctaccaaagc ggcggtaaat accgtaccta caacgtcgcc atcgaaggcg
     6181 cgagcctggt taccgtgtac cgcaaccaat tcggcgaaat catcaaagcg aaaggcgtgg
     6241 acggactgat tgccgagttg aaggctaaaa acggcagcaa gtaatccata agccatgccg
     6301 ccagactgaa gatggcatgg atttttccct accgaacact atgcatacag aactcaacaa
     6361 cggaacactg cacatcagcg gcgacgtaac cgtcaaaacc ctgactgccg ccgcctttac
     6421 ccgcttcaga cagcaatgtc gtctgaaaga cacccgcgcg gttgatttga gcggcgtcgg
     6481 acgcgccgat tccgcctgcg tgtcgctgct gctcgaagtg ctgcgcggtt gcaaaggcag
     6541 cgtcaggctg accggcattc ccgaatccgt gcgcgcgctg tccgaactgt acgaaatcaa
     6601 agactggttg aaatcatgaa aaaaaccgcc tacgccgccc tcctgctgat cgggttcgct
     6661 tccgcccctg catttgcaga aacccgcccc gccgaccctt atgaaggcta caaccgcgcc
     6721 gttttcaaat tcaacgacca agccgaccgc tacattttcg cccctgccgc gcgcggctac
     6781 cgcaaagttg cgccgaaacc cgtccgcgcc ggcgtgtcca atttttttaa caacctgtgc
     6841 gacgtggtca gcttcggcag caatatcttg cgcttggaca tcaaacgcgc aagcgaagac
     6901 cttgtccgcg tcggcatcaa caccactttc ggtttgggcg ggcttatcga catcgccggc
     6961 gcgggcggca ttcccgacaa taaaaacacc ttgggcgaca cgtttgcttc gtggggatgg
     7021 aaaaacagca attatttcgt gttgcccgtc ttagggccgt ccaccgtccg cgacgcgctc
     7081 ggcacgggta ttacctccgt ttattcgccc aagaatatcg tcttccgcac ccctgtcgga
     7141 cgctggggca cgactgccgt atccgccgtc agtacgcgcg aaggtctgct cgatttgacc
     7201 gacagtctgg acgaagccgc catcgacaaa tacagctaca cgcgcgacct ctatatgaaa
     7261 gtccgtgcgc ggcagaccgg tgcaacacct gccgaaggta cggaagataa catcgacatc
     7321 gacgaattgg tcgaaagtgc cgaaaccggc gcggcggaaa ctgccgttca agaagattcc
     7381 gtatccgaaa cacaggcaga agcagcaggg gaagccgaaa cgcaacctgg aacacaacct
     7441 taaagccatg ccgtctgaaa atccgtgttc ggacgacatt cccttctgtc ggaacggaca
     7501 gtccgaaacc acagattggg ctataatctc ttttttgcgc ataccgcgcg acagcaaagg
     7561 aaaaaccatg tacgaagtca accgcagcgt attcgtcctc atccctttag aacctttttg
     7621 gaactggctg caaaccctgc cgggcaacca cctcgacgga ctgactcttg aagacatcca
     7681 agccgacgcc aattcctacc ttgtccgccc gtgcgaaacc gccgacgaag tatgggacga
     7741 aatcgaagcc cgttttgaag acattttcgc cgccgaactt gccgactggt gcgaagacga
     7801 acgcgagtgg cctgcgctcg atgccgacat tttcaacgaa tggttcgaca tccagctttc
     7861 taccgtcatc accgacctcg aacacgaacc gcttgcccgc gaagccttcc aacccatcaa
     7921 cctgaactga tgaagctgac cgtccgcaac taccatctcg acggctacgg ccatgtcaac
     7981 aatgcgcgct acctcgaatt tttcgaagaa gcgcgctggg cgttttttga agaacgcgga
     8041 ctgctgcacg agttggcagg cctcatactg attgtcgccc gcatcgacat ccgatacagc
     8101 cgccccgccg tcgaaggcga cgtattgcag ttttcatgcc gcctgaaaac ccccggcacg
     8161 cgccgtattg tgctgaccca gacgattaca ctgccaaacg gcaaaaccgc cgcagaagcc
     8221 gacatcaccc tgatgcccgt ccacgccgcc acacaacgca ccgtcagcct gcccgccacc
     8281 cttgcccgcg cattggaagc cttgtccgaa tgaaaaaaat actcaccgcc gccgtcgtcg
     8341 cactgatcgg catcctcctt gccatcgtcc tcatccccga cagcaaaacc gcgcccgcct
     8401 tctccctgcc cgacctgcac ggaaaaaccg tttccaacgc cgacctgcaa ggcaaagtaa
     8461 ccctgattaa tttttggttt ccctcctgtc cgggttgtgt gagcgaaatg cccaaaatca
     8521 ttaaaatggc aaattactat aaaaacaaaa acttccaagt ccttgccgtt gcacaaccta
     8581 tcgatccgat agaaagcgtc cgccaatatg tcaaagacta cggtttgccg tttaccgtca
     8641 tgtatgatgc ggacaaagct gtcggacagg cgttcggcac acaggtttat ccgacttccg
     8701 tccttatcgg caaaaaaggc gaaatcctca aaacttatgt cggcgaaccc gatttcggca
     8761 aactctacca agaaatcgat accgcgctgg cacaatagcg atgccgtctg aagcgcaaaa
     8821 aaaacagaaa cgatgttcct gttttttcag atcatccgcc ccgacacgtt cagacggcat
     8881 tcaaccttcc ggcagattct ttttcaccac tgccggattg cctgccgcca aagagtaggg
     8941 cggaatgtct tttgtaacca ccgcgcctgc gcccacgacc gaaccgcgtc cgacggttac
     9001 gcccgccatt acaatcaccc tgtgccccgg ccagacatcg tcctccaacg taatcggacg
     9061 gatttccgtg tagccctcaa agcgtttttt gaacggtcaa acttgtggtt atttgaataa
     9121 aacagacatt ccggctccat catcacattt ttgccgacca ccagcccacg gcagatttca
     9181 cagtttgccc cgatgcccga gccgtcgccc aaaaccgtat ccggaaacac atacgccccg
     9241 cgttcgatat tgaccccgcg tccgatatgc ggagaaaccc gccgcgccaa aaaaccgcgc
     9301 acccgtctgc cgacgatgcc gatgccgcgc atatgtgacg gcggcaaccc cgaaccggca
     9361 aaacgcagca gcaggaaaaa gaattttgat cccatcaaac ctccctcaaa agaacttttt
     9421 a
//
LOCUS       DAWFBO010000074         9345 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFBO010000074 DAWFBO010000000
VERSION     DAWFBO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.074"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..329
                     /locus_tag="WGO87_001250"
     CDS             <1..329
                     /locus_tag="WGO87_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ0392607.1"
                     /translation="IATTVPAQQTFLDAAELMQWSIETEGLGLNVISHKILGKDHAQV
                     EFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGK
                     YLKPVA"
     gene            complement(548..1342)
                     /locus_tag="WGO87_001251"
     CDS             complement(548..1342)
                     /locus_tag="WGO87_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0392608.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1446..2171
                     /locus_tag="WGO87_001252"
     CDS             1446..2171
                     /locus_tag="WGO87_001252"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0392609.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFNGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGERMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
     gene            complement(3215..4057)
                     /locus_tag="WGO87_001253"
     CDS             complement(3215..4057)
                     /locus_tag="WGO87_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0392610.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
     gene            complement(4099..4194)
                     /locus_tag="WGO87_001254"
     CDS             complement(4099..4194)
                     /locus_tag="WGO87_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0392611.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            complement(4206..5045)
                     /locus_tag="WGO87_001255"
     CDS             complement(4206..5045)
                     /locus_tag="WGO87_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0392612.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHTVLWKQALDESLPYIAVFEDDVLLGEGAEKFLA
                     EDGWLKERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPSEGLHPVDWMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            complement(5087..6133)
                     /locus_tag="WGO87_001256"
     CDS             complement(5087..6133)
                     /locus_tag="WGO87_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0392613.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIARRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRPEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTPPAGAWLDFAADGRMRRLFTL
                     RQYFGILRRLLKNR"
     gene            complement(6150..8213)
                     /locus_tag="WGO87_001257"
     CDS             complement(6150..8213)
                     /locus_tag="WGO87_001257"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0392614.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNKTLGHRFLSNGEITIENADSYAAQMREQGKVVA
                     PFAERKAAIQTALERQARRLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTADPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALDADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTTFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            complement(8330..9226)
                     /gene="glyQ"
                     /locus_tag="WGO87_001258"
     CDS             complement(8330..9226)
                     /gene="glyQ"
                     /locus_tag="WGO87_001258"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0392615.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAVEEASLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLIKK"
BASE COUNT         2150 a         2530 c         2378 g         2287 t
ORIGIN      
        1 tcatcgctac taccgttccg gcgcaacaaa cattcctcga tgccgccgaa ctgatgcaat
       61 ggagtataga aaccgaaggg ctgggcttga acgtcatctc gcacaagata ctcggcaaag
      121 accacgccca agtcgaattt gaagcctact tccgagacgg acaacaccga tccgcgcatc
      181 acgaactgtc cggcttcgtc aacatcggcg gacaatggta ttttatcgat cccaccgttc
      241 cgcatcctgc gatgaaacaa ccctgcattt gcggatcggg caaaaaattc aaagcctgct
      301 gcggcaaata tctgaaacct gtcgcataaa aatgccgtct gaacgttcag acggcatttt
      361 caacgtgcaa aaaaaaacca ttcataccaa gggtaagtat gaatggtcaa tacattgcgg
      421 gaaaacgtct tacttgctgc actgccgaaa agggagaaac ggcagcggta atcagcggaa
      481 atgattgtac ccgaattaat gttaagaaac gttaatcgcg aaaatatatt aacaaacctg
      541 ttgaaaccta ttggttttcc cgtatccacc cgacccagcg ttcaaacagc ttcggttcga
      601 gcgcggcaac gaccgagcgt ttgaacacgt gttcaccact ccaaaacccg tcgccttcca
      661 aagtcgtcag cctgccgccc gcctcctcga aaatcaacgc acccgcagca tagtcccaca
      721 gcttctgccc accgtgaaca taaacatcat aacgcccgca cgccagataa caccaatcca
      781 acgtactgct gcccatactc cgtatcgttc caaaaggcgc gagcgtactc atacggctgg
      841 aaagtttgcc cgaacgcaaa tatttgattt ccacgcccgc aatcgcctca ttgagttttt
      901 tatccacgag acgcaaaggc aggcgggttc cattaagaaa cgcaccctgc ccccgttcgg
      961 cataaaaaca ttcgccgctg accggattgt aaatcacacc caattcggcg cgcccgttgc
     1021 ggacaaacgc caccgacacg gcaaaatgcg gcagcccgtt gacaaaattg ttcgtcccgt
     1081 ctatcggatc gacaatccac agcccctttt cccccgaata ttgttcccac aaagccgact
     1141 gttcctgccg cgacatttcc tcgcccaaca tcggactgtc gattaaaagc ggcaacgcgg
     1201 cggcaaaagc cgtctgcgcg gcaatgtccg cctcgctcaa catcgaaccg tcttccttgc
     1261 ggcaagacgg cgtattcaaa aaacgcggca taatttcggt ttgcgcgata tggcgcacga
     1321 ctttctgcaa acggtgcaac acttcctact gtcctcatat tttgaacttg cggcgcgcaa
     1381 acgtataatg tccgtttcca tcacgccgct gcgacggatt ataaccgtcc gaaccgccaa
     1441 aaactatgcc ccgattctac ctgcccgaaa acctttccgt cggacaaacc gtcgccctgc
     1501 ccgacaacat cgtccgccac ctcaacgtcc tgcgcgtccg ccccaacgaa aacatcaccc
     1561 tcttcaacgg caaaggcaag gcacacgccg cacagctgac cgttttggaa aaacgccgcg
     1621 ccgaagccga aatcctgcac gaagacacaa ccgacaacga gtccccgctc aacatcacgc
     1681 tcatccaatc catatcttcc ggcgaacgta tggactttac cctgcaaaaa agcgtcgaac
     1741 tcggcgtaac cgccatacag cccgtcatca gcgaacgctg catcgtccgc ctcgacggag
     1801 aacgcgccgc caaacgcctc gcacgctggc aggaaatcgt catctccgcg tgcgaacaaa
     1861 gcggcaggaa caccgttccc cccgtgctgc ccatcatcgg ctatcgtgaa gcactcgaca
     1921 aaatgccgtc tgaaagcacc aagctgatta tgagcatcaa ccgcgcccgc aaactcggcg
     1981 acatccgcca cccgtccggc gcaatcgtct ttatggtcgg gcccgaaggc ggctggacag
     2041 aacaggaaga acaacaggca tttgaagccg gctttcaggc ggttacactc ggcaaacgga
     2101 ttttacgcac agaaaccgcc ccactcgccg ccctcgccgc catgcagacg ctttggggcg
     2161 atttcgcata aacagaaatg ccgtctgaaa cccgttcaga cggcattttg cagccgatta
     2221 agatagtagg ttcaaataag atttcccccg ccgtcattcc cgcgaaagca agaatctaga
     2281 aacgaaaaac tacagggatt tatccgaaac aacaaaccct ctccgccgtc attcccgcgc
     2341 aggcgggaat ctagaaattt aacgttgcgg tgatttatcg gaaatgactg aaactcaacg
     2401 gactggattc ccgcctgcgc gggaatgacg aattttaggt tgctgttttt tggttttctg
     2461 tttttgcggg aatgacgaat tttaggtttc tgtttttggt tttctgttct cgcgggaata
     2521 acggaatttt aagttttagg aatttgtcgg aaaaacagaa atcccgccgc cgtcattccc
     2581 gcgaaagcgg gaatctagaa acgaaaaact acagggattt atccgaaaca acaaaccctc
     2641 tccgccgtca ttcccgcaaa agcgggaatc tagaaattta acgttgcggt gatttatcgg
     2701 aaatgactga acctcaacgg actggattcc cgcctgcgcg ggaatgacga attttaggtt
     2761 tctgttttca ggtttctgtt ttcaggtttc tatttttggt tttctgtctt cgtagaaatg
     2821 atgaaatttt aagttttagg aatttgtcgg aaaaacagaa atccccccac cgtcattccc
     2881 gcgcaggcgg gaatctggaa atttaatgtt gcggtgattt atcggaaatg actgaaactc
     2941 aacagactgg attcccgcct gcgtgggaat gacgagattt taggtttctg tttttggttt
     3001 tctgttttcg cgggaataac ggaactttaa gttttaggaa tttgtcggaa aaacagaaat
     3061 cccaccaccg ccattcccgc gcaggcggga atctagaaat ttaacgttgc ggtgatttat
     3121 cggaaatgac tgaaactcaa cggactagat tcccgcctgc gcgggaatga cagtgtatcc
     3181 atttctaatt ttaatccgct atattttaca caaactattt gaacgatatg acccgcctgc
     3241 cgtaagcttt ctcaagctcc gcctgccttt gacgctccat tcttttcttc ttttccctac
     3301 cgaatttacc caaagcacgc ttcaagtcaa acatcacctt caacgaacgg cggtgtcttc
     3361 tttcttgttc cctatctttt tccaaatcgc tacccaacat actgttttta ctgaggaact
     3421 tggcataatg caattcttgg gtacataagg cgggattaac ctgataaaca ggcatcccct
     3481 ccttatcaaa gaaataagta aacatcatcc aatctaccgc tttaatccac tctgccggca
     3541 aaacggcaaa cctttccaag aaaaaccgca tcgcctcacg cgaaatgata tagccagccg
     3601 tcccccaatg ttcgctctcc agcaaaggaa atgaccggtt ctcataattc aggactttat
     3661 ccggtctgac aataactttc gcaaacatcg tttccaaacg aacgataaag gcagaatcct
     3721 tatcaaaacg ctcttccaac caagtatctt cggcaaggaa cttttctgcg tctttgccaa
     3781 gcaggacatc atcctcaaat acggcaacat agggcagacc ttcatccaat gcctgtttcc
     3841 acaatacggc gtggctcata aagcaggctt tttccacttc gctcaacagg tgctgttttg
     3901 ccaatcccgg caccaattcc gccatcatcc gattcagttc ttcagacggc atcagtgcgt
     3961 cgaaaaactg aaacgggatg ccgtgcctgc cgaaggtatc ggcaatgtgc gccctgcgtt
     4021 ctgcggcgga agccaagctg ataacgtggt tttgcataat ttatcctgtt ttttgtctgt
     4081 tggataaaac ggcgtttttc aacggttttt cagcaatcgg tgcaaaatgc cgaagtattg
     4141 cctcaaggta aacagccgcc gcatcctgcc gtctgctgca aatacgatgt ccatcttttc
     4201 tccttttatt ggaaaggcac aataattttg ccgattaact gttcgcgcct ttgccggcgt
     4261 ttttcccttt ccctgctgat tttggtcaag gcgcggatca ggcggtgttt gaatgtgttg
     4321 gcgggggaat cgcgcctttg ctgtttgcgg ttcaggaggc ggtcgtgttc gatcaggctg
     4381 cccaatgcgc tgttttggtc gtgaaacttg gcataatgca gctcttgggc gcacaaggcg
     4441 ggattgagct ggcaaaccgg cattccttcc ctgtcgtcag ggttgccgaa catcatccaa
     4501 tcgacggggt gcagcccttc agacggcagg gcggcaaacc tgtccaggaa aaaccgcatc
     4561 gcttttcggg aaatgatata gcccgccgtc ccccagtgtt cgctttccaa aagcgggaag
     4621 gcacgcccgc agtagtccgc cacgccggag ggcgaggtca ggacgtgcat aaacatcgtt
     4681 tccaaacgga cgataaaggc ggaatccggg tcaaaccgct cttttagcca gccgtcttcg
     4741 gcaaggaatt tctccgcgcc ttcgccgagt aaaacgtcgt cctcaaatac ggcaatatac
     4801 ggtaagcttt cgtccagcgc ctgcttccac aatacggtgt ggctcataaa gcaggctttt
     4861 tccactccgc tcaaataggg gtgcgccgac aaaccgggga cgagttccgc cattgcctgc
     4921 tccagccttt cagacggcat cagtgcgtcg aaaaactgaa acgggatgcc gtgcctgccg
     4981 aaggtatcgg caatgtgcgc cctgcgttct gcggcggaag ccaggctgat aacgtggttt
     5041 tgcataattt atcctgtttt ttgtctgttg gataaagcgg cgtttttcaa cggtttttca
     5101 gcaatcggcg caaaatgccg aagtattgcc tcaaggtaaa cagccgccgc atcctgccgt
     5161 ctgccgcaaa atccagccac gcgccggcgg gcggcgtgtc cgtccgtttg aaacattgat
     5221 acaaaaaccg gcgggcgcgt tcaaaatctt cttccggcaa atgtttctcc agcaattcat
     5281 acgctactgc ttttatttgg cggtattcaa ggctgtcgaa ccgggtttta aaacccatag
     5341 actgcaaaaa atcgtttctg gcggtttttt ggatgccttg cgcgatttcg tgttggcgga
     5401 tgctgtattt ggatgaaacc tgattggcgt gaaggcggta tttgaccaag gcttcgggat
     5461 aataagccag cctgcccaat ttgctgacat cgtaccaaaa ttggtaatct tccgcccaat
     5521 cccgctcggt gttgtaacgc aaaccgccgt caatgacgct gcgcctcata atcatcgtgt
     5581 tgttgtgtat ggggttgccg aaagggaaaa agtcggcaat atcttcgggt cgggtcggct
     5641 ttttccaaat tttgccgtgc ctgtgatgcc gcgccagccg gttgccgtcc ttttcttccg
     5701 acaaaacttc cagccacgcg cccatcgcga tgatgctgcg gtctttttcc atctcgccca
     5761 cgattttctc aatccagtcg ggggcggcaa tatcgtcggc atcggtgcgt gcaatatatt
     5821 cccccattcc tgactttgcc aattcgtcca gcccgatgtt taaagaggga atcaggccgg
     5881 aattgcgcgg ctgcgcgagg atacggatgc ggccgtcctg ttcttggaaa cgccgggcaa
     5941 tggcaagcgt accgtctgtc gagccgtcat cgacaatcaa aatatccaag ttgcgccaag
     6001 tctgattcac gacggcggct aatgattggg cgaaatattt ttctacgttg taggcgcaaa
     6061 tcaatacgct gactaaaggc tgcaatttat tctcccgata ggcacgatgc cgtctgaagg
     6121 cttcagacgg catttggact gtacaacggt tactcgccca aaagcgcgat gtcggctacc
     6181 gcgttcattt gttctgccaa acggttcaac aggttcaaac ggttttgttt tacggcggca
     6241 tcttccgcca tcaccatcac gccgtcaaag aaggcatcga cttgcggctt gacggaagcc
     6301 agttcggaca aggcggtttg gaaattgcct tcggcgacgg cggcggcgat tttcggctgc
     6361 aagccttgcg cagcagcaaa aagggctttt tcttcgtcct gttgcaacag gctttcgtta
     6421 accgcgccca actcggcatc ggcttttttc agcaggtttt gcacgcgttt gttggcggcg
     6481 gcgagcgcgg cggcttcggg cagttgtttg aacgcggcaa cggcctgcag tttggcggtc
     6541 aaatcgtcca aacggcgcgg ctgtttggcg agtacggcgg caacgatgtc ttgcggataa
     6601 tcgttttgca gcaacacggc aaggcgcgcc tgcataaagt cggcggtttc agacggcgtt
     6661 ttttcgttga gcaaaccttt ggggaagctg tcgaaggtcg tctgaatcag ttcattcacg
     6721 tccaaaccat actgcatcag catccgcaaa atacccaagg cggcgcggcg cagggcgtag
     6781 gggtctttat cgccggtcgg aatcaggccg atgccccaaa ttccgaccag ggtttccagc
     6841 ttgtccgcca gtgccacggc ggcggcaatt ttgctttcgg gcagcttgtc gccggcaaaa
     6901 cgcggctggt agtgctgctc gacggcttcg gcgatttctt cggtttcgcc gtccaagcgg
     6961 gcatagtatt tgcccatcgt gccttgcaat tcggggaact cgccgaccat ttcggtaacc
     7021 aaatcggctt tggcaagacg cgcggcgcgc tcggctgcgg cggcatccgc gtccaacgct
     7081 ttagcgatgt gggcggcaat gctttgcaaa cgttcgatgc gttcggcctg cgaaccgatt
     7141 ttgttgtggt acaccacgtt tgccagcttg ggcaggcggc tttccagagt cgctttttgg
     7201 tcttgcttgt agaagaactc ggcatcagac aggcgcgcgc gcaatacgcg ttcgttgcct
     7261 tggatgatgt gcgacgggtc tgcggtttgc aggttggaga ccagcaggaa gcggttcatc
     7321 agcttgccgt tttggtcgag cagcgggaag tatttttggt tttgctgcat cgtcaaaatc
     7381 aagcattctt gaggcacggc gaggaagtgt tcctcaaaac cggcttccaa taccacaggc
     7441 cattcgacca gcgcggttac ttcgtccaac aaggcttcat cggcggcgac ggcggcgttc
     7501 agacggcgcg cctgcctttc caatgccgtc tgaatcgcgg ctttgcgctc ggcaaacgga
     7561 gcgacgactt tgccttgttc gcgcatttgt gcggcgtagc tgtcggcgtt ttcaatggtg
     7621 atttcgccgt tggagaggaa gcggtgtccc aaggttttgt tgccgctttg cagacccaaa
     7681 acgctgacgt tcacgacatc gccgccgtgc agcacaatca gcccgtggac ggggcgcacg
     7741 aaggtaaatg tgctgctgcc ccaacgcatt acttttggaa tcggcaattt tttcactgcg
     7801 gcattgataa tgtcttccaa aagttcgccc aacggtttgc cggtttggac gtattcgtag
     7861 gcgtacacgt cctgcttgcc gtcgtggacg atggtcaagt cttcgatttt cgcccctgca
     7921 ccgcgtgcga aaccttccaa agccttggtc ggcgcaccgt cttttacggc attcgccacg
     7981 gcagggcctt ttttcacgat tttctgatcg gcttgaacgg ctttgacgtt tttgacttga
     8041 accgccaaac ggcgcggcga ggcataagcc gtaaattcgg ctgcgccgtc aaccagttgc
     8101 gctttttcca agccttcggc aacggcggcg gcaaaatggt tgcccagatt attaagggct
     8161 ttgggcggaa gttcttcggt aaggagttcg attaaaaggg tttgggttgt catatgagac
     8221 tttctgtttg tccgtaggcc ctgcttataa agcctgcata taccgtacgg cctgtattaa
     8281 tggatacgca aaaaaggccg tctgaaaacc tgtttcagac gacgtatcgt tattttttaa
     8341 tcaacgggaa gcccagtttt tcgcggcttt cgacatattt ttgcgccacg gcgcggctca
     8401 acgcgcggat gcgcccgatg taggtggcgc gttcggttac ggaaatcgcg ccgcgtgcgt
     8461 ctaaaaggtt gaacgtatgc cctgctttga gaaccagctc gtaggcgggc agcgcaaggc
     8521 ttgcttcttc aacggcgagc aggcgtttgg cttgcgcttc gtagtcgttg aactggcgca
     8581 gcagccagtc ggcatcgctg tattcgaagt tgtaggtgga ttgctcgact tcgttttggt
     8641 ggtacacgtc gccgtaagtt actgtattcc cgtccagcgt ttttgcccaa acgaggtcat
     8701 agacgttttc tacgccttgc aggtacatcg ccaagcgttc gatgccgtag gtaatttcgc
     8761 cgagtacagg cgtgcagtca atgccgccga cttgttggaa ataggtaaac tgggttactt
     8821 ccatgccgtt gagccagact tcccagccca aaccccatgc accgagggtg gggttttccc
     8881 agtcgtcttc gacaaagcgg atgtcgtgga ctttgggatc gatgcccaat tcgcgcaggg
     8941 agtcgagata aaggtcttgg atattggcgg gggcgggttt aagggcgact tggaattggt
     9001 aatagtgttg caggcggttg gggttgtcgc cgtagcggcc gtctttgggg cggcggctgg
     9061 gttggacgta ggcggcaaac caaggctcgg ggccgagtgc gcgcaggcag gtggcggggt
     9121 gggaagtacc tgcgccgact tccatgtcga aaggttggat gacggtgcag cctttgtctg
     9181 cccagaatgt ttgcagtttg aagatgattt gttggaaggt aagcatggct tatgattcga
     9241 taaaataaag ggtttatttt actgtttcca ttgctgtttg gataggttta tctcaaagac
     9301 agactgattt gaaaacacgg catacatgat atagtggatt aaatt
//
LOCUS       DAWFBO010000075         9321 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFBO010000075 DAWFBO010000000
VERSION     DAWFBO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.075"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            164..1507
                     /locus_tag="WGO87_001259"
     CDS             164..1507
                     /locus_tag="WGO87_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0392616.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1510..2742
                     /locus_tag="WGO87_001260"
     CDS             1510..2742
                     /locus_tag="WGO87_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0392617.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2735..3511
                     /locus_tag="WGO87_001261"
     CDS             2735..3511
                     /locus_tag="WGO87_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0392618.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3511..4137
                     /locus_tag="WGO87_001262"
     CDS             3511..4137
                     /locus_tag="WGO87_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0392619.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTIFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4141..4734
                     /gene="nqrE"
                     /locus_tag="WGO87_001263"
     CDS             4141..4734
                     /gene="nqrE"
                     /locus_tag="WGO87_001263"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0392620.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4748..5965
                     /gene="nqrF"
                     /locus_tag="WGO87_001264"
     CDS             4748..5965
                     /gene="nqrF"
                     /locus_tag="WGO87_001264"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0392621.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMEIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6117..7172
                     /locus_tag="WGO87_001265"
     CDS             6117..7172
                     /locus_tag="WGO87_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0392622.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQTTADKPLRIS
                     SDFAHVTAEAIRLNRLTHGALDVTVGPLVNLWGFGPDKSVTQEPTPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            7198..7413
                     /gene="nqrM"
                     /locus_tag="WGO87_001266"
     CDS             7198..7413
                     /gene="nqrM"
                     /locus_tag="WGO87_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0392623.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7537..8115)
                     /gene="grpE"
                     /locus_tag="WGO87_001267"
     CDS             complement(7537..8115)
                     /gene="grpE"
                     /locus_tag="WGO87_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0392624.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAESAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8299..9117
                     /gene="cysE"
                     /locus_tag="WGO87_001268"
     CDS             8299..9117
                     /gene="cysE"
                     /locus_tag="WGO87_001268"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0392625.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2260 a         2605 c         2312 g         2144 t
ORIGIN      
        1 aacggcttcc gcaaggaaaa aagcagcaaa tctgacaaac aggtacaatt cctgatagaa
       61 tctaacactt tgctacatta atttattcaa tgcttttgat gccacgcgac acggtatcaa
      121 aacacgccgt ttttacctta accatcacaa gtaatgcaaa tatatgatta aaatcaaaaa
      181 aggtctaaac ctgcccatcg cgggcagacc ggagcaagtc atttatgacg gcccggccat
      241 taccgaagtc gcgttgcttg gcgaagaata tgccggtatg cgcccctcga tgaaagtcaa
      301 ggaaggcgat gccgtcaaaa aaggccaagt gctgtttgaa gacaaaaaga atccgggcgt
      361 ggtgtttacc gcgccggttt caggcaaaat cgccgccatc catcgcggcg aaaagcgcgt
      421 acttcagtcg gtcgtgattg ccgttgaagg caacgacgaa atcgagttcg aacgctacgc
      481 gcccgaagcg ttggcaaact taagcggcga agaagtgcgc cgcaatctga tccaatccgg
      541 tttgtggact gcgctgcgta cccgtccgtt cagcaaaatc cctgccgtcg atgccgagcc
      601 gttcgccatc ttcgtcaatg cgatggacac caatccgctg gcggcagacc ctgtggttgt
      661 gatcaaagaa gccgccgagg atttcagacg aggtttgctg gtattgagcc gtttgaccga
      721 gcgtaaaatc catgtgtgta aggcagctgg cgcagacgtg ccgtctgaaa atgctgccaa
      781 catcgaaaca catgaattcg gcggcccgca tccggccggt ttgagtggca cgcacattca
      841 tttcattgag ccggtcggtg caaacaaaac cgtttggacc atcaattatc aagatgtaat
      901 tgccatcgga cgtttgtttg caacaggccg tctgaacacc gagcgcgtga ttgctttggg
      961 tggttctcaa gtcaacaaac cacgcctctt gcgtaccgtt ttgggtgcga aagtatcgca
     1021 aattactgcg ggcgaattgg ttgacgcaga caaccgcgtg atttccggtt cggtattgaa
     1081 cggcgcgatt acacaaggcg cgcacgatta tttgggacgc taccacaatc agatttccgt
     1141 tatcgaagaa ggccgcagca aagagctgtt cggctgggtt gcgccgcagc cggacaaata
     1201 ctccatcacg cgtacgaccc tcggccattt cctgaaaaac aaactcttca agttcacgac
     1261 agccgtcaac ggtggcgacc gcgccatggt gccgattggt acttacgagc gcgtaatgcc
     1321 gctagacatc ctgcctaccc tgcttttgcg cgatttaatc gtcggcgata ccgacagcgc
     1381 gcaggcattg ggttgcttgg aattggacga agaagacctc gctttgtgca gcttcgtctg
     1441 cccgggcaaa tacgaatacg gcccgctgtt gcgtaaggtg ctggaaacca ttgagaagga
     1501 aggctgatta tgggcttgaa acattttctg gaaaaaatcg aaccgcactt cctgccgggc
     1561 ggcaaacatg aaaaatggta tgccctctac gaagctgcgg cgacgatttt ctatacatcc
     1621 ggtgcggtaa cgcgcaaagc ggcacacgtc cgcgatgcgc tcgactccaa acgcatgatg
     1681 attttggtgt ggctggcttt gttccctgcc atgttctacg gtatgtacaa cgtcggcgca
     1741 caggcattcg gtgccttaac gcccgatttg ctgcaacaaa gcatcgccaa cgactggcat
     1801 tacgcccttg ccaacgcttt gggcatcaat atgtcgtctg aagcgggcgt gttgggcaaa
     1861 atgctgttcg gcgcgattta cttcctgccg atttacgcga ccgtatttat tgtcggcggt
     1921 ttctgggaag ttttgttcgc caccgtgcgc aaacatgaaa tcaacgaagg tttctttgtt
     1981 acctcgattc tgtttgcctt aatcgttccg cccacgctgc cgctgtggca ggcagctttg
     2041 ggtatttctt tcggcgttgt ggttgcgaaa gaagtattcg gcggtacagg taaaaacttc
     2101 atgaaccctg cgctggcagg ccgtgccttc ctgttcttcg cctaccctgc caacttgagc
     2161 ggcgatgcgg tttggacggc ggttgacggc tattccggcg caaccgcgct ggcgcaatgg
     2221 gcggcacacg gtgcagacgg cctgaaaaac gccgtaaccg gtcaaaccat cacttggatg
     2281 gatgcgttta tcggcaaact gcccggctcc atcggcgaag tctccacttt ggcactctta
     2341 atcggcggcg cgtttatcgt gtttgcccgc atcgcttctt ggcgcattat tgccggcgtg
     2401 atgatcggta tgattgccat gtcttcgctg ttcaacttca tcggttcgga caccaacgct
     2461 atgtttgcta tgccttggta ctggcatttg gtcgtcggcg gcttcgccat cggtatgctg
     2521 tttatggcga ccgaccccgt ttccgcttcc tttaccaatg tcggcaaatg gtggtacggc
     2581 gcactgatcg gcgtgatgtg cgtattgatc cgcgtggtca accctgctta ccccgaaggc
     2641 atgatgttgg cgattctgtt tgccaacctg tttgccccga ttttcgacta ttttgtcgca
     2701 caagcgaaca tcaaacgcag aaaggcgcgc agcaatggct aagaaattcg ataaagacag
     2761 cttcagcggc acgctgattg tggtgttggc ggtcagcctg atttgctcgg tcatcgttgc
     2821 cggtgcggtc gtcggcttga aacccatcca agagaaacaa aaactccaag acaaacaagg
     2881 ctatatcttg agcgtagccg gtttgatgga taaggacacc gacatcggta aaacctttgc
     2941 cgagcgtatc gagcaacgtg ttgtcgattt ggcgaccggc gaatacgtcg ccgatgcgcc
     3001 taaagacttc agcgcgcgca tcgcaggcaa agaccctgcc caaagcatcc gcatcaaacc
     3061 cgaagacgat ttggcaggca tcaaaagccg tgccaaatac accgaggttt atttggtaaa
     3121 aggcgaagac ggcaaaatcg ggcaaatcat cctgcctatg cacggtaacg gtttgtggtc
     3181 ggtcatgtac ggctttgtcg ccatccagcc cgacggcaac accatcaacg gcattaccta
     3241 ctacgaacaa ggcgaaaccc cgggcttggg cggcgaaatc ggcaatccgt tgtggcagca
     3301 aaaattcgtc ggcaaaaaac tgtttgacgg acaaggcaaa ctcgccctgc acgtcggcaa
     3361 aggcgcgggt tcggacaaag aacacggcgt agatgccctc tccggcgcgt cgctgacatc
     3421 caaaggcgtg caaggttcgt tcgcctactg gttcggcgaa aacggctata tcccctacct
     3481 gaacaaattg aaatcagcag gagcgcaata atggctgata tgaaacgctt gaaacactta
     3541 atgttttcac cctttatcga caacaacccg attgccttgc aggttttggg tatttgttcg
     3601 gctttggcgg ttaccaccaa acttcagacg gccatcgtga tgggtatttc cgtagctttg
     3661 gtaaccggtt tttccagctt cttcatttcg ctggtacgca actacatccc caacagcatc
     3721 cgcatcatcg tgcaaatggc aattatcgcg tcgctggtta cgctggtcga ccaactcttg
     3781 caggcatttg cctacgaatt gtccaaacag ctttccgtat tcgtcggtct gattattacc
     3841 aactgtatcg tgatgggccg cgccgaagca tttgcgatga aagagccgcc gctggaaagc
     3901 ctgatcgacg gtatcggcaa cggcgcgggc tacgggatga tgctgcttgt cgttgccacc
     3961 gtccgcgaac tgatcggctc gggcaaactc ttgggctaca ccattttcca aaccgtgcag
     4021 gacggcggct ggtatcagac caacggcttg ttcctgctcg cccccagcgc gttcttcatc
     4081 atcggctttt tgatttgggg actgcgtacc tggaaacccg aacaggcgga ggaataagct
     4141 atggaacact atttgagcct cttcatcaaa tccgtcttca ttgaaaatat ggcgctatcc
     4201 ttctttttgg gtatgtgcac gtttttggcg gtatccaaaa aagtatccac cgcattcggt
     4261 ttgggtgtgg cggtaatttt cgtactcggg ctgtccgtcc ctgtcaacca actcgtttac
     4321 tcgctgctca aagacggcgc gattgtcgaa ggcgtggatt tgaccttttt gaaattcatc
     4381 accttcatcg gcgtgattgc cgctttggtg cagattttgg aaatgttctt ggataaattc
     4441 gtccccgccc tctacaacgc attgggcatc tacctgccgc tgattaccgt gaactgcgcg
     4501 attttcggcg ccgtatcgtt tatggcgcaa cgcgaataca acttcggcga gtccgttgta
     4561 tacggcttcg gcgcgggctt gggctggatg ttggcgattg tcgctttggc gggcattacc
     4621 gaaaaaatga aatattcgga cgctcccaaa ggcctcaaag gcttgggcat tacctttatc
     4681 gccgccggcc tgatggcgat ggcgtttatg tcgttctccg gcatccagtt ataagaaagg
     4741 gatcggtatg gagattattt taggtatcgt gatgtttacc gtcatcgttt tggttttggc
     4801 actgatgatt ctgtttgcca aatccaaact ggtcagcgaa ggcgacatca ccatcaaagt
     4861 caacggcgaa aaagagctga cgatgcccgc cggcggcaaa ctcttgggcg cgcttgccaa
     4921 cgaaggcatc tttatcccct ccgcctgcgg cgggggcggt tcgtgcggac aatgccgcgt
     4981 cgtcgtaaaa agcggcggcg gcgacatcct gccgaccgag ctgtcccaca tcagcaaacg
     5041 cgaagcacgc gaaggctgcc ggctgtcgtg ccaagtcaac gtcaaaaccg atatggagat
     5101 tgaagtaccc gaagaagtgt tcggtgtgaa aaaatgggaa tgcaccgtca tctccaacga
     5161 caacaaagcc acgttcatta aagaactcaa gctcgccatt cccgaaggcg aagaagtccc
     5221 cttccgcgcc ggcggctaca tccaaatcga agccccgccg cacaccgttg cctacaaaga
     5281 ctttgacatt cctaaggaat atcacgaaga ctgggacaaa tacaatttgt ggcaatacgt
     5341 ttccaaagtg gacgagccga ttttgcgcgc ctactctatg gcttcgtatc ctgaagaaaa
     5401 aggcatcatt atgctgaacg tgcgtatcgc cacgcctccc ccgcgcgtac ctgacgcgcc
     5461 tccgggacaa atgtcgtcct acatctggtc gctcaaaccc ggcgacaaag ttacgatttc
     5521 cggcccgttc ggcgaatttt tcgccaaaga caccgatgcc gaaatggtat ttatcggcgg
     5581 cggtgcgggt atggctccga tgcgttccca cattttcgac cagttgaaac gtttgaactc
     5641 caaacgtaag attaccttct ggtacggcgc acgttccaaa cgcgagatgt tctatgtcga
     5701 agactttgac caactcgcgg cagagttccc gaacttcacg tggcacgtcg ccctgtccga
     5761 cccgctgccg gaagacaact gggacggcta cacgggcttc attcacaacg tggtttacga
     5821 aaaccacctg aaaaaccacg aagcaccgga agactgcgaa ttttatatgt gcggcccgcc
     5881 gattatgaac cagtccgtca tcaaaatgct caaagacttg ggcgtggaag acgaaaacat
     5941 cctcttggac gatttcggcg gttaaatatt cggcttcaaa tacaaaagga agaaacctcg
     6001 gtttcttcct ttttctatcc gatgccgccc gaaccccctt cagacggcat cggcatagaa
     6061 acatatcggc cgttttgcga tataattcaa accatttgcc aaaaccgtca aacactatgc
     6121 cgtctgaaac acgcctgccg aactttatcc gcgtcttgat atttgccctg ggtttcatct
     6181 tcctgaacgc ctgttcggaa caaaccgcgc aaaccgttac cctgcaaggc gaaacgatgg
     6241 gcacgaccta taccgtcaaa tacctttcaa acaatcggga caaacttccc tcccctgccg
     6301 aaatacaaaa gcgcatcgat gacgcgctta aagaagtcaa ccggcagatg tccacctatc
     6361 agcccgactc cgaaatcagc cggttcaacc aaaccacagc cgacaaaccc ctccgcattt
     6421 caagcgactt cgcacacgtt accgccgaag ccatccgcct gaaccgcctg acgcacggcg
     6481 cactggacgt aaccgtcggc cctttggtca acctttgggg attcgggccc gacaaatccg
     6541 ttacccagga accgacaccg gaacaaatca aacaagcagc atcttatacg ggcatagaca
     6601 aaatcatttt gaaacaaggc aaagattacg cttccttgag caaaacccac cccaaagcct
     6661 atttggattt atcttcgatt gccaaaggct tcggcgttga taaagttgcg ggcgaactgg
     6721 aaaaatacgg cattcaaaat tatctggtcg aaatcggcgg cgagttgcac ggcaaaggca
     6781 aaaacgcgcg cggcgaaccg tggcgcatcg gtatcgagca acccaatatc atccaaggcg
     6841 gcaatacgca gattatcgtc ccgctgaaca accgttcgct tgccacttcc ggcgattacc
     6901 gtattttcca cgtcgataaa agcggcaaac gcctctccca tatcattaat ccgaacaaca
     6961 aacgacccat cagccacaac ctcgcctcca tcagcgtggt cgcagacagt gcgatgacgg
     7021 cggacggctt gtccacagga ttattcgtat tgggcgaaac cgaagcctta aagctggcag
     7081 agcgcgaaaa actcgctgtt ttcctgattg tcagggataa aggcggctac cgcaccgcca
     7141 tgtcttccga atttgaaaaa ctgctccgct aaacatacaa accgattagg aaacaccatg
     7201 aaaaccctgc tccttacctt cggcatcttc ctgaccgtca tcatcggtat ggcggtcggc
     7261 tatattttct ccaaacgcag catcaaaggc agttgcggcg gcattactgc cttgggtatg
     7321 aaaaaaatgt gcgactgcga cacaccttgc gacaccctgc aaaagaaact ggatgaagaa
     7381 aaacaggcag gcggcatacg ggttgaccgt taacgccgaa acaaaatgcc gtctgaagcc
     7441 cgacaggcgg gttcagacgg cattgccgct ttacttcgat cggtttatac gcaaaacgcg
     7501 cttcaggaaa taaatcagat tattccccag acgccttcag gcttccttct gtgccaccgt
     7561 aaccatagcc gggcgcaaca cgcggtcgga cagcgtataa cccttcttca tcacgcccac
     7621 cacggtattg ggttcctgtt cgcttgccac cgcctgcatc gcctgatgga tattcggatc
     7681 gagcttatcg cccgctttag gattgatttc cttgatttgc gtagcatcaa atgctttctg
     7741 caactcgttc aaagtcatct gcacgcccat tttcagcgca tcgaaattgc cgctctgatc
     7801 caaaagcgcc atttccaaat aatccttgac cggcagcatt tccacggcaa acttctgtcc
     7861 ggcgaacttg tgcgtatccg caatttcctg ctggtggcgg cggcgcaggt tttgctcgtt
     7921 tgccaaagcg cgcagctgct cgtctttcaa ctgcgcttcc agctcggcaa tccgcgcctg
     7981 caaatcctca taagccgact ctgcggcagc ctgttcctgc acaccgtccg catttcctac
     8041 tgtctcgacg gtttccaccg cctccacatt ttcaaccgct tcttcactgt tttgctgctg
     8101 tgtctgttcg ctcatatcgt atcccttaaa acaaattgga aatcaaaatc cagttattac
     8161 ccgcgcaaga taaggacatt ttcaagaaac ttcaagcaaa agcagagaat ttcaaattca
     8221 ttttcaaatc ccctaccgaa aaaataatat agacggtaag atactgccga ccatatcgat
     8281 tttcaaacgt catcactcat gaaaaaagac catttaaaca caaccggttt cgacctctgg
     8341 cacaccatcc gcgaagaaac cgcggccgcg gtctccgccg aaccgatgct ggcaagtttt
     8401 ttgcaccaaa ccgtgttgcg ccacgagtcc ctcggctccg tccttgccta ccacctttcc
     8461 agcaaactcg gcagcccgat tatggacgtg cgtgcgctgt ttgaaatcta ccagcaggcg
     8521 ttgggtagcg acacccaaat cggcaaatgc gtcgaagcag acttaaaagc catctacgaa
     8581 cgcgatcccg cctgcgacga atattcgctg ccgcttttat atttcaaagg cttccacgcg
     8641 attcaggcac accgcatcaa ccaccggctg tatctcgacg ggcgcaaaac gctggcgtat
     8701 ttcctgcaaa accgtatgtc cgaagtattc ggcgtggaca tccatcccgc cgcccgtttc
     8761 ggatacgggc tgatgctcga ccacgccacc ggctttgttg ccggcgaaac cgccgtgttg
     8821 ggcaacaata tttcgatttt gcacggcgta acgctcggcg gttcgggcaa agaaggcggc
     8881 gaccgccacc ctaaaatcgg cgacggcgtg atgatcggcg caaacgcctc gatattgggc
     8941 aatatccgca tcggcagcaa tgccaaaatc ggggcgggca gcgtcgtggt ttcagacgtg
     9001 ccgccgtcca tcacggttgt cggcgtaccc gccaaacccg tggcgcgatc gctcaaaaca
     9061 ccgtcggcgg atatggatca aaatatccag tttaccgaaa tcgactttat gatttgaata
     9121 tccgcaccaa tgccgtctga aacgccaaac gggcttcaga cgacattgcc cgcttcaagc
     9181 ctgcgccttg atatgccgcc gatgtcggca cttgccagtc ggacaaaatg ccttttattc
     9241 aatacacccg attcccacgc aaccggaaat atagtggatt aacaaaaatc aggacaaggc
     9301 gacgaagccg cagacagtac g
//
LOCUS       DAWFBO010000076         9299 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFBO010000076 DAWFBO010000000
VERSION     DAWFBO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.076"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(91..1371)
                     /locus_tag="WGO87_001269"
     CDS             complement(91..1371)
                     /locus_tag="WGO87_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0392626.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRSCVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1593..2213)
                     /locus_tag="WGO87_001270"
     CDS             complement(1593..2213)
                     /locus_tag="WGO87_001270"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0392627.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WGO87_001271"
     CDS             complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WGO87_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0392628.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3352..3924
                     /gene="ampD"
                     /locus_tag="WGO87_001272"
     CDS             3352..3924
                     /gene="ampD"
                     /locus_tag="WGO87_001272"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0392629.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4118..5404
                     /locus_tag="WGO87_001273"
     CDS             4118..5404
                     /locus_tag="WGO87_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0392630.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5552..8077
                     /gene="ligA"
                     /locus_tag="WGO87_001274"
     CDS             5552..8077
                     /gene="ligA"
                     /locus_tag="WGO87_001274"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0392631.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGLITEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTVVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDIVVVRRAGDVIPEVVRVIFERRPMQETDVAASDGIGHRQDDLFAET
                     PSANQTQTVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQTPSED
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSILSEAELLTLLG"
     gene            8110..>9299
                     /locus_tag="WGO87_001275"
     CDS             8110..>9299
                     /locus_tag="WGO87_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0392632.1"
                     /translation="MSTFPNHTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFPAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLARLGRVHN
                     GKEALTAIDTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGTLEGAGFIHYETSAFAQNNKQCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPAAMLQERTGVPSAKIMAQIETARQKGLLETNPAVFRPTEKGRLFLN
                     DLLQ"
BASE COUNT         2205 a         2816 c         2322 g         1956 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactatatc aatctgacag caaaatgccg cctgaaaaca
       61 gttcagacgg cattttaaac cgcaaacggc ttacagcttc cattcgctca gcttggcagc
      121 gtaagcttcc aaatctgcaa tcggacgggt tgccacgccg ctttccatcg ctgctttggc
      181 ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac ggagtcggaa tcaggtattc
      241 cgcgccgaat tcaaatttct taccgtaagc ggcaaccact tcttcggtta cttcttccat
      301 cgccaaatcc gccaaagcat acacgcagga gcgtttcatt tcttcgttga tggtcgtcgc
      361 gccgacatcc aacgcgccac ggaagatgaa tgggaagcac aatacgttgt tcacttggtt
      421 cgggaagtcg gaacggccgg taccgataac cacgtccgga cgggtttctt ttgccagcgg
      481 cggcaggatt tccggattcg ggttggccat ggcgaacacg atgggttttt cgttcatcgt
      541 gttcaacatt tcgggggtca gcaggttcgc gccggagagg cccaagaaga tgtctttacc
      601 tttgacggca tcggcaagta cgcgctggcc attgtcttca acggcgtaga attttttgga
      661 ttcgtccatg cgatctttgt cttcgcgggt ttggtaaatc acgcctttgg agtcgcaaac
      721 ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg ttcaagcagg caatcgcggc
      781 cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt ttacggccgg taaaacgcag
      841 ggcgttcaat acggcagcgg cggtaatgat ggccgtgccg tgctggtcgt cgtggaatac
      901 ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag aaacactcgg gtgctttgat
      961 gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg gcgatgatgt ccacgagttt
     1021 ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc acaccggcga attttttgaa
     1081 caatacgcct ttgccttcca tcacgggttt gcccgccagc gcgccgatgt cgcccaagcc
     1141 caaaacggcc gtaccgttgg aaatgacggc gaccaagttg cctttggcgg tgtatttgta
     1201 ggcattttgc ggatcggcat ggatttccat acaaggagcg gctacgcccg gagagtacgc
     1261 caacgccaaa tctttgtcgg tcgccaaaga tttggtcggg gtaacggaaa ttttgcccgg
     1321 aacgggtaat tcgtggaact tgagggcggc ttctttcaat gagttttcca tgtgtcaatc
     1381 ctgttgcgag aaaaagattt gaatcggact gtccgaaacg ctttcgggtc atcatgttac
     1441 gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa ccgagcgtaa ttctatcatg
     1501 ccgaaagttt ggcgcatacc gccattttca cctgcggcac tgccgtttga ttttcggcgc
     1561 gggtggatgc gcttgtttca atatttgccg cattaacagc cgaaatgtcc gtccaacact
     1621 ttttctatgt tgtttctgac ttcatccaag ctgcggttac tgtcgataat ggcgtaccgt
     1681 tcgggacagg cggcggcacg gttcagataa acgctgcgca cacgcatgaa gaaatctgcc
     1741 tgttcctgct cgaaccggtc tttctcgcgc gactgtccga tacgcgccat cgacacttcc
     1801 agcggcacat ccaacagcag ggtcaaatcg gggcgcaaac cgccctgcac ccaatgttcc
     1861 aaaatttcaa tgtcttcaga cggcatcccc cgcccgccgc cctgataggc gaaggttgcg
     1921 tcggtaaaac ggtcggacac gacatggatg ccgtctgaaa gtgcaggcaa tatcacgtct
     1981 tcaatatgct gcattctggc ggcgaacatc atcagtgttt ccgcacgcaa accggctttg
     2041 gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga ccggcgttcc gcccggttcg
     2101 cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg ccttgatgac ggcaaggttg
     2161 gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga attgcggttt catgcttgca
     2221 gttccggtaa gggtatgccg tccgaaaaca caaacttttc agacggcatg gtttattttt
     2281 tcaaaatata tttgcgaacg gcggcgttgt gttcggtcaa atcatggctg aactggctca
     2341 agcccgtacc gtccattttg gacacgaaat acaggtattt ttcaccggac ggatgggcgg
     2401 cggcatcgag tgccgccttg ccgggcagcg cgatcggggt tggcggcaga ccgccgcgcg
     2461 tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt acggattttg cccttgtatg
     2521 ccgcacccat gccgtaaatc acggacgggt cggtttgcag gcgcataccg attttcaggc
     2581 ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc atgccctgtt tccttttcga
     2641 tcaggctcgc cataatcagc atttcataag ggtttttata aggcagcccg tcctgcctgc
     2701 tttcccatgc ctcattcagt cggcgttgca tcgccttgta ggcgatttgg taaatccgta
     2761 aatcgctgcc gcccgcatcg atttcgtagc tgtcggggaa aaactgccct tcaggattgc
     2821 cgctgaaggc atcaggggca acttccgcca tcagtttttc attgctccag cctttggtgt
     2881 cgtgttcgat gtcgggcgtt gcgtcgatga ctttcctcat atgcgaaaaa cgcgaacctt
     2941 cgataatctg cacggtaacg gaatccggcc tgccgccgcg cattttctgc aagatatccc
     3001 aagcagacac ttccgaaggc agtctgtacg tccccgtatg cagcctgttg tgcacaccca
     3061 aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac gatgcggtct tcggcaagtt
     3121 tcctgccgac cgacgaaata ccctggtttt tggcaatttt aatcctgtat gccctgccgt
     3181 tgtctttagg gacgaaaagc agcgcggcga aaacggctgc cgatacggtc aaaaaaacgg
     3241 cagaccattt caacaatttt ctcaacatgg taggattccc agcattcgga atacgtctta
     3301 aaattcgggc gacagtataa cccaaattgc ccgccggaca ggaagaacat catggacaat
     3361 cacgccgaag cacactggca aaacggctgg cttcaaagca tacgccatac cccgtcgccc
     3421 aatttcagcc cgagggaaac gggggaaacg gtttccctga tcgtgttgca caacatttcg
     3481 ctgccgccgt tcgaatatgg tacggatgcg gtggaaaagc tgtttgccaa ccggctcgac
     3541 cccgacggac atccgttctt cagcctgata cacactttgc gcgtatccag ccatttttta
     3601 atcaaacgcg acggcgaaac ggtacagttc gtttcctgcg acaatatggc ataccatgcc
     3661 ggcgtatcct cgtttcgcgg acgggaaaaa tgcaacgcat tttccatcgg catcgaactg
     3721 gaaggctgcg atttcgaacc ctttaccgaa gcgcaatacc gttcgctcga agccttgttg
     3781 gacgcaatct gccgccgcta ccccatcacg gcggtaacgg ggcaccaaga catcgcgccc
     3841 ggccgcaaaa ccgaccccgg tcattttttc gactggcggc ggatacgggg gaaagggttt
     3901 cccgtggaca gaaatgccgt ctgaaaacca cagattttag atggcatttc ggaaaatttc
     3961 cgcttatttc gacacactgc tgtcagaccc tgcaattttc gttataattc aaacgttata
     4021 accggccgtt ccgtcagact gccggaaacg caagcccgat ttaaccgcag tacccgcact
     4081 ttgcgcatcc tgcatcccga aatttaaagt gattgccatg atttacatcg tactgttcct
     4141 cgccgccgtc ctcgccgttg tcgcctacaa tatgtatcag gaaaaccaat accgcaaaaa
     4201 agtgcgcgac cagttcgggc actccgacaa agatgccctg ctcaacagca aaaccagcca
     4261 tgtccgcgac ggcaaaccgt ccggcgggcc agtcatgatg ccgaaacccc aaccggcggt
     4321 caaaaaaacg gcaaaacccc aagaccccgc catgcgcaac ctgcaagaac aggatgccgt
     4381 ctacatcgcc aagcagaaac aggcaaaagc ctccccgttc aaaaccgaaa tcgaaaccgc
     4441 cttggaagaa agcggcatta tcggcaactc cgcccacacc gtttccgaac cccaaaccgg
     4501 acattccgca ccgaaacctg ccgacgcgcc ggcaaaacct gcacccgttc cgcaaacacc
     4561 tgcaaaaccg ctgattacgc tcaaagaact gtcaaaagtc gaattaccct ggtttgacgt
     4621 gcgcttcgac ttcatctcct atatcgcgct gaccgaagcc aaagaactgc acgcactgcc
     4681 gcgcctttcc aaccgctgcc gctaccagat tgtcggctgc accatggacg accatttcca
     4741 gattgccgaa cccatcccgg gcatccgcta tcaggcattt atcgtgggta ttcaggcagt
     4801 cagccgcaac ggacttgcct cgcaggaaga actctccgca ttcaaccgcc aggtggacgc
     4861 attcgcacaa agcatgggcg gtcagacgct gcacaccgac cttgccgcct ttatcgaagt
     4921 ggcttccgca ctggacgcat tctgcgcgcg cgtcgaccag accatcgcca tccatttggt
     4981 ttccccgacc agcatcagcg gcgtagaact gcgttccgcc gtaacgggcg tgggtttcgt
     5041 tttggaagac gacggcgcgt tccactatac cgacacgtcg ggctcgacca tgttctccat
     5101 ctgctcgctc aacaacgagc cgtttaccaa tgcccttttg gacaaccagt cctacaaagg
     5161 cttcagtatg ctgctcgaca tcccgcactc tccggcaggc gaaaaaacct tcgacgattt
     5221 gtttatggat ttggcggtac gcctgtccgg ccagttgaac ctgaatctgg tcaacgacaa
     5281 aatggaagaa gtttcgaccc aatggctcaa agacgtgcgc acttatgtat tggctcgtca
     5341 gtccgagatg ctcaaagtcg gtatcgaacc gggcggcaaa accgcattgc gcctgttctc
     5401 ctaaaatcga atgcaatcaa agcggatatg tcgttacggc atatccgctt tttttcgggt
     5461 aaaattcagc tgttttcaac ctatcatttt tcagacgacc tttgcccgtt tcatcccgta
     5521 tcagacaaag gtcgtctgaa aaccttctct catgaatcca aactcaaaac ataacactaa
     5581 ctttaccaat cttcttaaac cgtctgatag tgatataaaa caatttgccg cacaacacat
     5641 ccgccacctc accgacctgc tcaaccgcta tgcctacgaa tactacaccc tcgacgcgcc
     5701 cagcgtaccc gatgccgaat acgacaaatt attccgcgag ctcgaagcgt tggaacgaaa
     5761 ccatcccgag ctcaaactgc ccgacagccc gacccagcgc gtcggcggcg agcctctggc
     5821 gggatttgcc gaagtacgcc acgaagtgcc gatgctgtcg ctgaccaacg ccttttcccc
     5881 gcaagatgaa aacggcgtgt tcgaccatgc agaaatgtac gctttcgacc aacgcgtgcg
     5941 cgacggtttg gacggcggca aacccgaata cgttatcgaa cctaaattcg acggcctcgc
     6001 catcagcctg ctctaccgcg acggcgtatt ggtacaggcg gcaacgcgcg gcgacggcac
     6061 aacgggcgaa gacgttaccc aaaacatcaa aaccgtatcc aacatcccct tgcggctgca
     6121 cggcgaaaat acgcccgaac tcatcgaagt acgcggcgaa gtactgatgc aaaaagccga
     6181 ttttgccgcc ctcaataaaa gacaagccga aaacgggcaa aaaccctttg ccaatccgcg
     6241 caacgccgcc gccggcagcc tacgccaact cgattcgcgc atcaccgcgc agcgcaaact
     6301 gcactttttc ccctactccg ttgcccgcca gcaaggcggt ttgattacgg aagaacacat
     6361 ccaagaactc gcctatttcc aagcactcgg cttcagtctg cccaatggca atttcggctg
     6421 tttcaaaaat atcgatgaag tattggcgtt ttacgaacaa atgcagcaaa aacgccccga
     6481 actgccctac gaaatcgacg gcatggtcgt caaagtcaat agcttggcac aacagcgcga
     6541 actcggcttc atctcccgcg cgccgcgctg ggcgattgcc cacaaattcc ccgccgaaga
     6601 agccctgacc gtcgtcgaag cgattgacgt gcaaatcggg cgcaccggcg ccgttacccc
     6661 cgtcgcccgc ctgcaacccg tattcgtcgg cggcgtaacc gtaaccaacg ccaccctgca
     6721 caatcaggat gaagtatcgc gtaaagacgt tcgcgtcgga gacatcgtcg tcgtgcgccg
     6781 cgccggagac gtgattcccg aagtcgtgcg cgtgattttt gaacgccgcc cgatgcagga
     6841 aaccgacgtt gccgcttcag acggcatcgg gcatcggcag gacgatttgt tcgccgaaac
     6901 gccgtccgcc aatcaaaccc aaaccgttcc gctccacaag ccctaccgcc tgccgaccca
     6961 ctgccccatc tgccgcagcg aaatcgaacg cgaagaaggc gaagccgtcg cccgatgcag
     7021 cggcggcatg ctttgtcagg cacaacgcgc gcaaggctta atccacttcg cctcgcgcaa
     7081 agcgatggac atcgacggct tgggtgaaaa acaaatcgaa cagcttgtcg cccaagacct
     7141 cgtccgccac ttcgccgacc tctaccgcct cgatattccg accctgcaaa aaatgaagga
     7201 aacggcggac aaaaccgttg ccgaatcgga tcaaacgccg tctgaagaca gttcggtcgg
     7261 cgcgtcgggc aaacataaaa aacaaccgac caagtgggcg gaaaacatcc tcgcaggcat
     7321 agaatccggc aaaacacccg aactcgcccg cttcctgttc gcgctcggca tccgccacgt
     7381 cggcgaacgc accgccaaaa cgctggcgca ggcattcggc acattggaac gcgtccgccg
     7441 cgcccccgaa cccgtcctcg cctgcctgcc cgacatcggt acggtagtgg cgcattccat
     7501 tgcccacttc ttcgcccaag ccgaacagca ggcgatgata gacgaactgc tcgccgcagg
     7561 cgttgccccg caaacccaag ccgttaccat cccgcccgcc cgccacgccg aaccgcaacg
     7621 ctggatcgcc cgcctgcccg acttcaaaat tagcgaaaac aaagcccaag ccttatggga
     7681 actcgctggt aaaaacatag aaggtctaca aaacgacaaa gccctccccg ccgactggca
     7741 agcatggcgc agcgaaccac aaaacgccgc cctactcgaa aacctgaaaa ccttcttcgc
     7801 gcaaatgccg tctgaagacg aggcggcgca aggttcagac ggcatcaata aagccgtagc
     7861 gggcaaaacc ttcgtgttaa ctggcacact gcccaccctc aaacgcgacc aagcccaatc
     7921 cctaatcgaa gccgcaggcg gcaaagtttc cggcagcgtt tccaaaaaaa ccgattacgt
     7981 cgtcgctgga gaagccgcgg gcagcaagct ggaaaaagcc aatgccttgg gtgtttccat
     8041 cctcagcgaa gccgaactgc ttaccctgct cggctgaacc tgtgccgtct gaaccgcctt
     8101 cagacggcat tgtccacttt tccgaaccac accatgcaca ccatttcctt tccaaaccgc
     8161 acccgactca ccgccctgcc gcccctgtcg ctttacatcc acatcccgtg gtgcatcaaa
     8221 aaatgcccgt attgcgactt taattcccac agcctgaaaa acggattgcc cgaagccgcc
     8281 tatatcgacg cgctgctgac cgacttgcag cttgaattgc ccaatatttg gggcagaccg
     8341 gtggaaacga tctttttcgg cggcggtacg cccagcctgt ttccggcgga atcgattgac
     8401 cgtttgttga gcggcgtgcg ttcgctgttg cgcttgcagc cggaagcgga aattacgttg
     8461 gaagccaatc cggggacgtt tgaaatcgag aagtttcaag gatttaaaga cgcaggtatt
     8521 acgcggcttt ctattggcgt gcagagtttc aacgacgata tgcttgcgcg gctggggcgc
     8581 gtccacaacg gcaaagaagc cttaaccgcc atcgacaccg ccttgaaatt atttgataaa
     8641 gtcaatatcg acttgatgta tgccctgccg aaccagaccg ttcagacggc attggacgac
     8701 gtacaaaccg ccatcgctac gggcgcgacc cacatcagcg cgtatcatct gacgatggag
     8761 ccgaacacgc cgttcggtca tacgccgccg aaaggtttgc cacaagacga agccgccctc
     8821 gacatcgaag acgcagtaca cggcacgctg gaaggcgcgg gttttatcca ctacgaaaca
     8881 tcagcctttg cacaaaacaa caaacaatgc cgccacaatt tgaactactg gcagttcggc
     8941 gattatttag gcataggcgc gggcgcgcac ggcaaaatct cctatcccga ccgcatcgag
     9001 cgcaccgtcc gccgccgcca ccccaacgac tacctcgcct taatgcaaag ccaaccgagt
     9061 gaagccgtcg aacgcaaaac cgttgccgcc gaagatttgc cgttcgaatt catgatgaac
     9121 gccctgcgcc tgaccgacgg cgtgcccgcc gcgatgttgc aggaacgcac gggcgtaccg
     9181 agtgccaaaa tcatggctca aatcgaaacg gcaaggcaaa aaggtttatt ggaaaccaac
     9241 cccgccgtgt tccgcccaac cgaaaaagga cgcttgtttt taaacgattt gctgcaatg
//
LOCUS       DAWFBO010000077         9264 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFBO010000077 DAWFBO010000000
VERSION     DAWFBO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.077"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(45..3077)
                     /locus_tag="WGO87_001276"
     CDS             complement(45..3077)
                     /locus_tag="WGO87_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ0392633.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGSSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3242..3922
                     /locus_tag="WGO87_001277"
     CDS             3242..3922
                     /locus_tag="WGO87_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0392634.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3951..4310
                     /locus_tag="WGO87_001278"
     CDS             3951..4310
                     /locus_tag="WGO87_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0392635.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            complement(4963..5499)
                     /locus_tag="WGO87_001279"
     CDS             complement(4963..5499)
                     /locus_tag="WGO87_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0392636.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAESVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5533..5889)
                     /gene="folB"
                     /locus_tag="WGO87_001280"
     CDS             complement(5533..5889)
                     /gene="folB"
                     /locus_tag="WGO87_001280"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0392637.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGIPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            5945..6547
                     /gene="plsY"
                     /locus_tag="WGO87_001281"
     CDS             5945..6547
                     /gene="plsY"
                     /locus_tag="WGO87_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0392638.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILLLLRHKSNILNLIKGKESKIGEKR"
     gene            6635..7480
                     /locus_tag="WGO87_001282"
     CDS             6635..7480
                     /locus_tag="WGO87_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0392639.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYWRVTETLKSALKRFESGCYLIWYPCLSREESRKLPEELKKLVPD
                     NYLHAELHIHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            7729..8703
                     /locus_tag="WGO87_001283"
     CDS             7729..8703
                     /locus_tag="WGO87_001283"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0392640.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(8862..9080)
                     /locus_tag="WGO87_001284"
     CDS             complement(8862..9080)
                     /locus_tag="WGO87_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0392641.1"
                     /translation="MFPEYRDLISRLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2153 a         2705 c         2156 g         2250 t
ORIGIN      
        1 gccgtctgaa acagtcttca gacggcattt ccatttgcaa aaaatcaagc attgtccaag
       61 gggacgagaa tcgtacggtt gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc
      121 atctggtcaa tcagacgcgc ggcgcggttg tagccgatac gcaaggcgcg ctgtacgccc
      181 gaaatactgg ctttgcgcgt tttcaggaca acggatacgg cttcatcata catcggatcg
      241 gtttcgccgt cgccgctgcg cccgatgccg ggcagctctt cgctgctgcc gccgctcaaa
      301 atatcgtcaa catagtccgg ctcgccaaac tgtttcaaat attcgaccac gcggtgcacc
      361 tcttcatccg aggcaaacgc gccgtgaacg cgctgcggat aggcagtacc cggcggcagg
      421 aacagcatat cgccctgacc gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg
      481 ctgtcgattt tgctggacac ttggaacgcg atacgcgtcg ggatgttcgc cttaatcaga
      541 cccgtgatga catcgacgct ggggcgttgt gtggcaagga taagatggat gcctgccgcg
      601 cgggcttttt gggcgaggcg ggcaatcagt tcctcgattt tcttgcctgc cgtcatcatc
      661 aaatcggcaa actcatcgac cacgaccacg ataaacggca gtttttccaa aggttcggga
      721 tcgtcgggcg tgaggctgaa cggattgccg attttttctc cccttgctgc ggcttcggcg
      781 attttttgat tgaagcccgc aagattacgc acgcccataa agctcatcag gcggtagcgt
      841 ttttccattt cgttaacaca ccagttcagc gcgtttgccg ccagcttcat atcggtaacg
      901 acaggggcga gcaggtgcgg gatgccttcg taaatgctca attccagcat tttcggatcg
      961 atcataatca tacgcacgtc ttccggcgcg gctttgaaaa gcatagacag aatcatcgcg
     1021 ttgacaccca ccgatttgcc cgaaccggtc gtgccggcaa ccaacaaatg cggtgctttg
     1081 cccaagtcgg ttacgacggg ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg
     1141 gatttggatt cggcaaactc gggcgaattg aagatttcgc tcaggcgtat catttggcgt
     1201 ttcgggttcg gaagttccaa acccatacag gttttgccgg ggatggtttc gacaacgcgg
     1261 atggaagcca cgccgagcga acgcgccaag tctttttcca gatttagaac ggaattgccg
     1321 cgcacgccga catcgggttc gatttcataa cgcgtaatca cggggccgga ataagaatcg
     1381 acaaccttga ccttgacttt gaactccgcc aatttttctt cgatggtgat gctgttttcc
     1441 aacagttctt cttcggtttg cgtcgcctcg ggattgaaca gcggcggcag aagcaggtcg
     1501 gttgtcggca ggtgttcgta taccgcatcg gtttcttcag attggaacgc cccttcatcc
     1561 gcttccgtat ccgatacccg gcaggacggg cgttcagacg gcacatcttc aaacggacaa
     1621 atcgcctgac tgtcatgccc gaacgcttcg gtttccgaca gatattgccc gtttgaccgc
     1681 tctgccgcac cttcactgcc gtcatctgca atagcggcgg tttcctcctg ccaacctcca
     1741 ttcaaaacat catcggcaag atggtcggtt tcagcaatgc ggctgcgttg cgcctgctcg
     1801 aatcctgtcg gcggctcata ggtacggttg tagatttccg atacgggagg cggcggcgga
     1861 atatcgattg cgggcatggg aactttcggc acttcgggtg gttcgaccac ccaagcatcg
     1921 ggcgcagcag gttcttcaat atagatatcg gcagcttcat cataaaccgg accgccgtat
     1981 cccacagacg aaacggtttc cgtgaaaaca ttttcggttt cagacggcat ttcgacatcc
     2041 cgtttcccga caaccgtccg actttccgga atcaggcgcg aaatccgcgc ctccgcagaa
     2101 accgttgcct tgttcggttc tttcaaattg acggaaacgc ggcggcaggc atcgcggacg
     2161 gctgtgccgt ctgaaacgga ctgtccttgg gaaacatccg gggattttgc ctccgcctgc
     2221 cctgcatgac ggcggaacgg cgcattccgt gcattttcgg ctttgactgc agaaaaggca
     2281 gacggatgct gcttctcaaa atacggctca aatccgtaat ccgcagactc ggaaaacgct
     2341 tctttgtccg catcgaacat acgggaatac gtgcgttgaa gcacagggtc gtccaaaccg
     2401 ataatctgta gcccttccat cggggtatcg gacacgcgga ctttggggac cgctgtttcg
     2461 tttttctcaa atgcatcgat atagcgtttt ttcgtttccc ttaaagccgc gctgtttaaa
     2521 gcacgcgttg cttcttccaa agtgatttcc ttaaaaaccg gacggacggg cgaaatttcg
     2581 ctttcagacg gcatcaaccc ttcagcaata ctccggtcga atgggatgcg gcggttgtcg
     2641 attacggcag ttgcaatgtc ttccgtatcg gcagcctctt cctccgcagc ttctgcttct
     2701 tccgtttccg cttcttccgt cccgtttcca ctgtctgaat atccgtcgga tgcggtcgga
     2761 acatccgcac ggtttgcaga agcaggcgga acgggcctgt ccggttcgga ttcgtgcgcc
     2821 agatagtttc ggcagaaacg gacgacaccg acaaacagcc aatacactgc cgtcttgacg
     2881 gcatggaaaa gcatcagggc aagttcggga aaatcgggca taccgtcttt gatttcaggc
     2941 agctccgcct gtttttcccc tttcttttcc tgccacgcag aaacctcgcg catccactcg
     3001 cgttcggatt gtgcgcggac aaaaaacaag ccggcaagcg caagcaacaa aataacgatc
     3061 ataactatcc aaaacatatg ccgtttcccg caagacaaac gattaaacgg gtttatttta
     3121 acgtgtttcc atcccaaaaa aaacaaccgg tcggcaattc tgccgaaacc gattcggctt
     3181 tcggtttgcc cccgccgaca gtataatagc cgtcctttcc cttaatcttt gcggcattgc
     3241 catgattttc caaacagtct ggttttcaga tatggtactg tcggtttcgt ggattgtcct
     3301 tatcctgata ctggcagctt ccgcgccgtc ggcattccgc tcgcttgccc gctaccgttc
     3361 cgcccttcca ctgtgtaccg taattttttc cgcagcatgg tgtctgaacg cctctgccgg
     3421 cggcggacag cttgcacaga tgaactacca cctgctcgcc gtcaatctgg ttgcactgat
     3481 ggtgggcact tccgccgcct tttggcttgc cgcgctgctg atgctgccct actgcctgct
     3541 gtttgccggt tctgccggcg catatccgcc gaacgcttta gtgctgattc tgcccgcgct
     3601 tgccgtcaac cgcctgtcgc gtatgctggt caaccgcctg ccgcccaata ttttcatctt
     3661 tattttcgtc aacggctttc tcgcttccgc cgccggcatt ctgttgaccg ggctggtgct
     3721 gaccggtatt ttggatgctg caaacgcttt tccgtccgaa atattgtgga cgaccgccct
     3781 gcccgtcttt attttgctgg cgtgggcgga agccttcctc agcggcattt caactgccat
     3841 atttgttgca ctgaagcccc attggattaa cactttcgac gacaaccgct atcttaagtc
     3901 cgaccgcggc atatggcggt agccttcaga cggcatagac aggcaaaacc atgctttcca
     3961 acatcatccc cctttccatc ggtgcggcac tcggtgcaac ggcgcgttgg ctgctcaacc
     4021 tcgccgttcc cgcatcgttg tctcccgcca cgggcaacct gtttgccaac tggacgggcg
     4081 cgctgctcat cggcatcttt gccgaaaccg tcaaccatcc gcaatggaag ctgctgctga
     4141 ttaccggttt tctcggcagc ctgaccacgc tttccggatt ttcactggaa accgtaaccc
     4201 tgctccaatc gaatcgtcct gcttcagcac ttgccaatat tttcctgcac acggcaggtt
     4261 cgctgctgct gacttggctc gggctgaaga tagggacggc agtcaaataa ccgcaacaaa
     4321 ttgcctccct ttgttttgac ataatctata aattagcttc gcttgtttaa atactggctg
     4381 tgccaattat agtagattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
     4441 atagtacgga accgattcac ttggtacttc agcaccttag agaatcgttc tctttgagct
     4501 aaggcgaggc aacgccgtac tggtttttgt taatccacta taccgttctc aaattcaaat
     4561 tgggctttta ggtctataac aaagaaaaag ccttggtagg ataaacctac caaggctttt
     4621 tcttaaaaaa acgctctatt accgtgccta aaaatgcaaa aaagtaaccg tcataaccct
     4681 tttgccaaaa tcaatatcat catccgatga atgaattgac ggtatataag aaagaattaa
     4741 taagctcata aaccggtaag aaaaatcagt aacaaagata ttcattatta accattttaa
     4801 aaattatcat gttcgtttgg gtaattatta tacccttttc cctcttatta ctaaagcacg
     4861 aatgacttta aaggacgcgc ccagagacaa gccgaaaagg catatctcat ccgccaaaca
     4921 aaatgcccac cgacttactc cggcaggcag ttcaacaccc tgtcaatcct tcatcagcca
     4981 gtattgcaaa ccgattaatg tcttgccgtc tttaatttca tcgtttgcca atgcctgacg
     5041 gacttcttct ttcgacatca atacggtttc cgtaatctcg tcttcgtcat tggcaagcgt
     5101 actgcccaaa cgcacgcttt ccgcttcgaa cagatacatt ttttcgttgc aaaaaccgac
     5161 cgccgtataa aaactgtaaa gcaggcgtac gctgtcggcg gtataaggcg tttcctccgc
     5221 caattctcgc agcgcacacg ctgccatatc ctcacccgcc acatccagct tgcccgcagg
     5281 aagttccaat gtcgcctgat ttgccgcata acgccactgc cgcaccaaaa cgaccttttc
     5341 atcatccgtt accgccaaca cgcacgccgc acccggatgg cggatgacga tacgttgccc
     5401 ttcattgccg ttgggcaacc tgaccttatc cctgctgatg ctgacgaaac cgccctcgta
     5461 aatggtttcg ccgcctaatt ttacttccct caaatccact tcatatctcc tttccgtttc
     5521 ctattatgcc gttcaatctt cacgcttgcc gcgctcgatt tccacgccaa cctcgcgcac
     5581 gccttctaaa atacccggct tgacgatttt cacgcgtacc cacaccgcgc cgaaatatcc
     5641 caaaaccaaa tcggcaatat attccgccaa cgcttccaaa agcaggaaat cctgttcttt
     5701 cagatgtcgg cgcaatgttt cgcatacctc ggcataatgc accgtattgg caatatcgtc
     5761 gtccgaaccc gctttctcgg gaatgccgat gtccaaatcg acaatcaggg tctgcaaccg
     5821 ttcgcgttcc cagccgtaca caccgatgag cgtatccgcc ttcatgccgt gcaaaaagat
     5881 tttatccata atccgccccg ttttttgcta aaataaccgc accattctaa gtaaagtacc
     5941 gcaaatgttc aacataccgg ctgttgccgt ttcctatctg atcggctcac tttctttcgc
     6001 cgtcatcgtt tccaagtatt acgggatgga cgacccgcgc acctatggtt cgggcaatcc
     6061 gggggcaacc aatgttttac gcagcggcaa aaaaaaggcg gccgcgctga cgctcttggg
     6121 cgatgccgcc aaaggtttgg ttgccgtttt gcttgcacgc gtgcttcaag aaccgctcgg
     6181 tttatccgac agcgcaatcg cggccgtcgc actcgccgcg ctggtcgggc atatgtggcc
     6241 ggtgtttttc ggatttaagg gcggcaaagg cgtggcaacg gcattgggcg tgcttctggc
     6301 actctctcct gcaactgcct tggtctgcgc gttgatttgg cttgtgatgg cattcggctt
     6361 caaggtgtcc tcccttgccg cattaaccgc cacaatcgcc gccccccttg ccgcactgtt
     6421 ttttatgccg catacttctt ggattttcgc aaccctcgca atcgccatat tgctgttgct
     6481 ccgccataag agcaacatcc tcaacctgat taaaggcaaa gaaagcaaaa tcggcgaaaa
     6541 acgctgattc ggcatttgtt ttccattacg tcatgccgtc tgaaaccatg ttcccgcgtt
     6601 cagacagcat ttccgcacaa acggctttca taccatgctc agttacagac acgcattcca
     6661 cgccggcaac cacgccgata tgctcaaaca ctttaccttg tttttagtgc tgcaatattt
     6721 caaccgcaaa gacaagccct actggtacat cgacacgcac ggcggcgcgg gtgtgtacaa
     6781 tctcgaaggc agcgaggcgc agaaagtcgg cgaataccgg caaggcatcg ccctgctccg
     6841 acaggcgcaa aacctgcctg ccgaactctc tgactttgcc gcgcacatac aaaaaatcct
     6901 gccctcgccc gaactttact gcggttctcc gtggctggca caatcgctga cccgcgtcgg
     6961 cgacaaatta cgcctgtttg agctgcaccc caccgacttt gtccatctgc aaaacaatat
     7021 gggcgaagca ggattgggca aacgcggaca agtgttgcgc gaagacggtt acaaagggct
     7081 gatttccctg ctgccgcccc cgccgcgccg tgccgccgtc ctcatcgacc cgccttacga
     7141 ggaaaaacag gactactggc gtgtaacgga aacgctgaaa tccgctttaa aacggtttga
     7201 gtccggctgt tatctcatct ggtatccctg cctaagccgc gaagaaagcc gcaagctgcc
     7261 cgaagaactg aaaaaactcg ttcccgacaa ctaccttcac gccgaactgc acatccacgc
     7321 gccgaaagcc gacggtttcg gtatgcacgg cagcggaatg ttcgtcatca atccgcccta
     7381 tctgttggcg gaacaattgg ctgcaaacct ccccgccctg acgcggctgc tggcgcaaga
     7441 cgaaggcgcg cgctacctgt tggacagcaa aatccgctga ccccgatgcc gtctgaaaca
     7501 acccttcaga cggcatctcc cgtcaaacaa acaaaaccgg acgctatacc ggcagtcggc
     7561 acttttaaga ataaatatcc cctcatccgc ctgccgcgtt ccgacttgcc ccatgccgtt
     7621 tttttacact acaatcccct agactatcct gcaaaacgca ccaaaggaat acgccacaat
     7681 aacctgccgc ctttcagacg gcacaaacct ttccgaaacg agcaaactat ggacacactg
     7741 acccgattcc tccccgaaca cctgcaacaa aaccagctgc ccgaagcact cggcggcgtt
     7801 ttactgtccg ttgtttccgc ctgcaccgaa atcaacgcca aagtccgcct cggcgcactg
     7861 gccggcgtat tgggtatggc gggcacgggc aatattcagg gcgaagacca aaaaaaactg
     7921 gatgttatcg ccaacaacat catgattgac acactcaaag ccaaccctgc cgttgccggt
     7981 ttggcaagcg aggaagaaga ctctttcgta agtgccggtg aaaacggacg ctatctcgtc
     8041 ctattcgacc ctttagacgg atcgtccaat attgatgtca acatttccgt cggtacaatt
     8101 ttctccatcc ttgaaaaacc cgaaggcgca ttggcaaccg aatcattcct tcaaacgggc
     8161 agacagcagc ttgccgcagg ctatatcctc tacgggcctc aaacccagct cgtattcaca
     8221 ttcggacacg gcgtatatat gttcacgctc aatgccgaaa acgaatttgt gctgaccaaa
     8281 gaaaaaccga aagtacccga aagtaccaaa gaatttgcca tcaatatgtc caaccgccgc
     8341 cactggctgc cccccgtcca acaatacatc gacgagctct tggcgggcga aaccggcacg
     8401 cgcggcaaaa actacaatat gcgctgggta gccagcatgg ttgccgaaat ccaccgcata
     8461 ctgatgcgcg ggggcgtgtt catgtatccg caagacaaac gcgacccgtc caaacccggc
     8521 aaactgcgcc tgatgtatga agccaatcct atggccctga tactcgaaca agccggcgca
     8581 tcggcaagca acgcccgtca ggacatactc ggcatccgtc ccgaaagcct gcaccagcgc
     8641 gtcgccgtca ttatgggcag cagcgaagaa gtggattacc tcaaccgcct gcattcaaaa
     8701 taaaaggcag cctgaccgat aaagaaaggc ggcagcgcgc atccgacgct gccgccgata
     8761 tttcgtttat agccttaaaa agatagaaaa ccacctttcc gaacggcaaa aatgccgtct
     8821 gaaaagtttt cagacggcat tttaaaaatt aaagcccgaa tttattttcc cgctgctttt
     8881 tgcaggatgg cgtacaactc gtctttcagt ttcaatttgg cttttttcag ctcatcgatg
     8941 gtttccgcac cgctggtaac cggattgttg accagaccgg taattttatc gtccagctcg
     9001 ttgtgttcgt cgaacagacg ggcgaagcgg gaattttcct gtttcaatct ggaaatcaaa
     9061 tcacggtatt ctgggaacat atcgctttcc tctcttggtt tacggttaaa aagtcaaact
     9121 gaatctttac attaattata acaaagataa tatttttatc cagtgtttta tcggatacgg
     9181 caaaattttc ttttaacacc gccgtccggt tgataaaaac gttgacaata tcacgcttta
     9241 cgcataaatt ttcagccttt ccta
//
LOCUS       DAWFBO010000078         9192 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFBO010000078 DAWFBO010000000
VERSION     DAWFBO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.078"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WGO87_001285"
     CDS             complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WGO87_001285"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0392642.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WGO87_001286"
     CDS             complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WGO87_001286"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0392643.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(3195..3896)
                     /locus_tag="WGO87_001287"
     CDS             complement(3195..3896)
                     /locus_tag="WGO87_001287"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0392644.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            4082..5194
                     /locus_tag="WGO87_001288"
     CDS             4082..5194
                     /locus_tag="WGO87_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0392645.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            5228..6205
                     /locus_tag="WGO87_001289"
     CDS             5228..6205
                     /locus_tag="WGO87_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0392646.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            6209..6559
                     /locus_tag="WGO87_001290"
     CDS             6209..6559
                     /locus_tag="WGO87_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ0392647.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            6564..7343
                     /locus_tag="WGO87_001291"
     CDS             6564..7343
                     /locus_tag="WGO87_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0392648.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRYTAEHIAKLRNQTLEQVAAYTTENFYRLFQKVPDMRTV"
     gene            7369..7584
                     /locus_tag="WGO87_001292"
     CDS             7369..7584
                     /locus_tag="WGO87_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0392649.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            7685..7996
                     /gene="grxD"
                     /locus_tag="WGO87_001293"
     CDS             7685..7996
                     /gene="grxD"
                     /locus_tag="WGO87_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0392650.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            8102..8728
                     /gene="upp"
                     /locus_tag="WGO87_001294"
     CDS             8102..8728
                     /gene="upp"
                     /locus_tag="WGO87_001294"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0392651.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLATELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            8751..9071
                     /locus_tag="WGO87_001295"
     CDS             8751..9071
                     /locus_tag="WGO87_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0392652.1"
                     /translation="MNFQDYLATFSSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
BASE COUNT         2237 a         2373 c         2389 g         2193 t
ORIGIN      
        1 gtaggaaaat ccgacaacgt tagacccgcc tgcaaaggta aggaatagca aatgccgtct
       61 gaaactattt tcagacggca ttcttggctt cctggcctaa cggattgccg taccggccct
      121 gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg ccgacaacca gcttgtcatc
      181 cacaaatccc cagtagcgag aatcggcact gttgtcgcgg ttgtcgccca tagcgaaata
      241 gcgtccttcg ggaactttgc acacgaaacc gctgccgtcg tcggcatatt ggcagtgttc
      301 caaaccgctt tgctctatgg aatatccgtt ttcagacata atatcggagg tatatttgcc
      361 caatacgggc agggaaacgg caggctgtcc ttcttttttc agaatattga aggatttgcc
      421 gtccaaaccg ctgtggaaca tatccgtatt gtggatttcg gatgggtcgg tgtcgtcggg
      481 ataacggtat gtgccgttag gaatatcgga agtgggtttg ccatttaccg tcaaaatctt
      541 atcccgatat tcgaccacat cgcccggaat gccgacaata cgcttgatgt aggtcatctc
      601 cggctgcaga ggataattaa aaacaacgac atcgccccgt tcgattttgc ctgtggggat
      661 aaatatattg tttaaaacgg gtacgcgcaa gccgtaggaa aatttgccga ccaaaatgaa
      721 atcgcccttg atcaggcccg ggcgcatgga gctggatgga atttggaacg gttcggcaat
      781 aaacgaccgg atgagaaaca ataccaaaac ggtagggaag aaactgccga aataatcgcc
      841 gaagtggctg ctttccgaga tttcgggatg agtcttcagg cggtatttgt atacccccca
      901 agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg gtaaagctca taaacaggga
      961 caaagcggca aacacgccga ccgctgtcag gatataggcg tattcgaggc cggaactcca
     1021 ttcaccgttt tcctgccgct tcttgtcgct tttgaaataa aggatgatgc cggcaagcag
     1081 cgcggcagcc gcgcccgaca ttagcattgt gttcattgtt gttccttaat gcttaaaaac
     1141 ccgcctgtcc gtgcaaccgt tttaaggcgg caaattgcaa aatttgtttg cgggcgcgtg
     1201 cccctgaaat tagggcggtt tgaggggtgt tcccgacgcg ccgccctgtg tgccagagtt
     1261 atttgtcgct cacctgcaaa atcgccaaga acgcgctttg cggaatttcc acattgccca
     1321 cttgtttcat acggcgttta cctgcctttt gtttttcaag cagttttttc ttacgcgtaa
     1381 tatcgccgcc gtaacatttc gctaagacgt ttttacgcag tgctttgacg ttttcgcggg
     1441 cgataatctg gctgccgatg gcggcttgga cggcaatgtc gaacatttgg cgcggaatca
     1501 gctcgcgcat tttcgatgcc agctcgcggc ctcggtgaac cgcgctttga cggtgcacaa
     1561 tcaggcttaa tgcatcgact ttttcgccgt tgaccataat atccagctta atcaaatcag
     1621 acggttggaa ctctttgaaa tggtagtcca acgaagcata accgcgcgaa gtggatttga
     1681 gtttgtcaaa gaagtccatg acgacttcgt tcatgggcaa atcgtaagtc agcatcactt
     1741 ggcggcccat gtactgcata ttgacctgaa cgccgcgctt ttggttacac aaagtcatga
     1801 cgttgccgac gtattcctgc ggcacaagga tggtcgcggt aataatcggc tcgagtatgg
     1861 tttcgatgct gccgatgtcg ggcagtttgg acgggttttc aacttcgatt ttctcaccgt
     1921 ttttcaacac gacttcataa accaccgtcg gcgcggtggt aatcaaatcc atatcgaact
     1981 cgcgctccaa gcgttcctgc acgatttcca agtgcaacag acccaagaag ccgcaacgga
     2041 agccgaagcc caatgcctgg gaaacttcag gctcgaattt caacgaagcg tcgttaagct
     2101 gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg gctttccacg ggataaagtc
     2161 cggcgaatac ttggctttgt acttcttgga agccgggcag cggctcagtg gcagggttgg
     2221 caaccagtgt aaccgtatcg ccgactttcg cctgacccaa ttcttttacg ccggtaatca
     2281 aaaagcccac ttcgccggct ttgagttctt gtttttgaac tgattttggt gtgaatacgc
     2341 ccagctgctc gacctgcgtt tccgccttgg tgctcataaa gcgcactttg tctttcagtt
     2401 tgatggtgcc gtttttcact cgaatcagca tgaccacgcc gacatagttg tcaaaccacg
     2461 aatcgacgat aaccgcttgc agcggcgcgt tttcgtcgcc ggtcggcgca gggattttgg
     2521 caacaatttc ttccaaaaca tcttccacgc cgataccgct tttggcggaa cactgtactg
     2581 caccgacggc atcgatgccg atgatgtctt cgatttcctg ttccacacgc tcggggtcgg
     2641 cggcgggcag gtcgattttg tttaaaacag gcacaacctc cacgcccaaa tcaatcgcgg
     2701 tgtaacagtt cgctacggtt tgcgcttcca cgccctgcga cgcgtcaacg accagaagcg
     2761 cgccttcgca agccgacagc gaacgggaaa cttcgtaaga gaagtcgacg tgtcccggcg
     2821 tgtcaatcaa attaagctga tacacctgcc cgtcgcgcgc tttatagttg agcgcggcgg
     2881 tttgcgcttt gatggtaatg ccgcgctctt tttcgatgtc catggaatcg agtacctgcg
     2941 tactcatttc gcgcaaatcc aaaccgccgc aatattggat gaagcggtcg gcaagcgtcg
     3001 atttgccgtg gtcgatgtgg gcaatgatgg agaaatttcg gatatttttc attagagttg
     3061 ttttgaatgt cggacagtgg gtttgggaaa tgccgtctga acaaacggcg ttgcgtccga
     3121 atatcgggtg caacgtggaa atagcccgat attctaacgg aaaaccgctg ttttggcatg
     3181 atttgataaa ggttttataa agatttgacg atttctgcca ccatttttgc agaacttgcc
     3241 gccgccgttt tcaaaaattc gtcaaagctg atgtctgcct tttcgtccgc cgaatcggaa
     3301 accgcgcgga tgatgacgaa aggcgtttcc aactgatgac aggtttgggc aatcgccgcc
     3361 gcttccattt ccactgcttt gactttgggg aagtgcttgc ggatttccgc cacgccttcg
     3421 ctgctgtgga cgaagcggtc gccgctgaca atcaggcctt gttctaccgc cgcgccttca
     3481 aacacttccg ccgcctgttt tgctttgccg accaagaggc cgtctgaaac aaatacggcg
     3541 ggcagttgcg gcacttgtcc ccaaacatag ccgaatgcgg ttacatcgac atcgtggtgc
     3601 gcgatttctg tgccgaccac gacatcgccg actttcaaac ccttgcccaa gccgcccgcg
     3661 ctgccggtgt tgataacgca gtccggtgcg aattgatgga taagccaagc cgttgcaacc
     3721 gccgcgttga ccttgccgat gccgctcaat gcaagcacca tgcgttttcc cgctaattcg
     3781 ccttcatagg cggcaaactt gccgaaagag acggctttga cattttccat catctcgcgc
     3841 aaaagctcga tttcttgttc cattgcgccg ataacggcta ctgttttcaa agacatattg
     3901 ctgacctgtt gtgaatttcg gatagaatgc ctgattatac acgctaacac ggcaggattg
     3961 agtggaggtg gtttgtccgt gccgtctgaa acggtttcag acggcacggc gggtttttgg
     4021 tagaatggga aggtacagat tgtttgaaga ttaggggacg aggatgttta ccgatgaaaa
     4081 tatgaccgca aaggaagaac tgttcgcatg gctgcgccat atgaacaaaa acaaaggttc
     4141 cgacctgttt gtgacgaccc atttcccgcc cgcaatgaag ctggacggca aaatcacccg
     4201 catcacggac gaaccgctga cggcggaaaa atgtatggaa atcgcctttt cgattatgag
     4261 tgcgaagcag gcggaagaat tttcatcgac caacgagtgc aacttcgcca tcagcctgcc
     4321 ggacaccagc cgcttccgcg tcaatgcgat gatacagcgc ggcgcgacgg cgttggtatt
     4381 ccgtgcgatt accagcaaga ttcccaagtt tgaaagcctg aacctgccgc cggtcttgaa
     4441 ggatgtcgcg ctgaaaaaac gcgggctggt tatttttgtc ggcggcaccg gctcgggcaa
     4501 atcgacttcg cttgcctcgc ttatcgatta ccgcaatgaa aattcgttcg gacatatcat
     4561 caccatcgaa gacccgatcg agtttgtcca cgaacacaaa aactgcatca tcacccagcg
     4621 cgaggtcggc gtggatacgg aaaactggat ggcggcgttg aaaaacacgc tgcgtcaggc
     4681 gccggatgtc atccttatcg gcgaaatccg cgaccgcgaa acgatggact acgccattgc
     4741 ctttgccgaa acggggcatt tgtgtatggc gacgctgcac gccaacagca ccaatcaggc
     4801 actcgaccgc atcatcaact ttttccccga ggagcggcgc gaacaattgc tgacggattt
     4861 gtcgctcaac cttcaggcgt ttatttcgca acgcctcgtt ccgcgagacg gcggcaaggg
     4921 cagggtggcg gcagtcgagg tgctgctcaa ttcgcccctg atttcggagt tgattcacaa
     4981 cggcaacatc catgaaatca aagaagtgat gaaaaaatcc actaccctgg gtatgcagac
     5041 cttcgatcaa cacctttacc aattgtatga aaaaggcgat atttccctgc aagaagcatt
     5101 gaaaaatgcc gattccgcac acgatttgcg tttggcggta cagttgcgca gccgccgcgc
     5161 gcaaagttcc agccccgatt tggaactgct ctgatggcgg tatggatttc cggacggatg
     5221 gtttgaaatg atttatccgt ggcataatga gcaatggcgg cagattgcgg aacattggga
     5281 gcgtcgtccc aatgcatggc tgtttgccgg caaaaaagat acggggaaaa ctacatttgc
     5341 ccgctttgcg gcgaaggcac tgttgtgcga aacccctgca ccgggctgca aaccctgtgg
     5401 cgaatgtatg tcctgccatc tgtttggaca gggaagccat cccgattttt acgaaatcac
     5461 ccccttgtcg gacgaacccg aaaacggacg caaactgttg cagatcaaaa tcgatgccgt
     5521 cagggaaatc atcgataatg tgtacctgac ttcggtacgg ggcggtttgc gcgtgattct
     5581 gattcatcct gcggaaagta tgaatgtcca agccgccaac agtttgttga aagtgttgga
     5641 agagccgccg ccacaagtgg tctttttgct ggtcagccac gcggcggaca aggttttacc
     5701 gaccattaaa agccgctgcc ggaagatggt tttgcccgcg ccttcccatg aagaggcatt
     5761 ggcatatctg cgtgaaaggg gtgtggcgga acctgaggaa cgtctggctt tccattccgg
     5821 agcgccgctg tttgatgagg cggacggtgt ccgtgcgttg cggattaaac tgttggatat
     5881 tttggcagaa ccaaggttgt tgaagatttt ggattacgcc gcgcttttcg ataaggaaaa
     5941 acttccgctc gccgtatttg tcgggtggat gcagaagtgg ctggtcgatt tgggattgtg
     6001 cctgcaacac atgaaacccg tctattatcc cgcttatgaa gacaggctgc ttcagacggt
     6061 atccggtttc cgtccgcgca atgtatttgc ggcggaggat atgctcaaac agcttgcccc
     6121 ctacgggttt catactttaa atgtcaaaat gcagatcgag catctgctca tcaactattt
     6181 ggaattgaag aaagagaacg ggtgaattat gtcagacgga caaaatattc cggcaaaaat
     6241 gatgtcgttg cagctgaaag acatgaatct gctgtacagc tcctacatgc cgtttttgga
     6301 acacggcggt ctgtttgtgc agaccaacga cgtattttcc atcggggacg atattctgct
     6361 tgccgtagaa atcctcaact tccccaaact gttcctgccg accaaagtcg cctggatcaa
     6421 tcctgcgcgt acttcctcca aacccaaagg ggtagggctg gcattcacaa aacacgaaaa
     6481 ctgcctgaaa gtcaaagatc agatcgaagt cgaactgggc aacacaatcg gcggcagcag
     6541 acctacgttt accatgtaac gccatgcata tcatcgattc gcactgccac ctcaattttg
     6601 aaggtttgaa agaacgcctg cccgaagttt tgtccaacat ggaagcaaac ggcgtggggc
     6661 aggcgctcgc catcagcgtc agcagggaaa gcttctccga agtctttgcc atcgccgaag
     6721 cgcacgaaca catctattgc accataggcg tacatcccga cagcaaggaa gccgaagaat
     6781 tttctattgc ggaaatggtt gccgcagccg cccatccgaa agtggtcggc attggcgaga
     6841 cgggcttgga ttattactgg tgcaaaggcg atttgtcctg gcagcacaaa cgctttgcag
     6901 accacatcga agcagccaat caaaccggac tgcccgttat cgtccatacg cgtgatgcgg
     6961 cggcggacac cttgtctatc ctgaaagaat gccgggttaa ttcgggcgtt atccactgtt
     7021 tttccgaaga catcggtttt gcccgtgcag caatggattt ggggctttat atttctttct
     7081 cgggaatcgt tacctttaaa aacgcaccct tggttcagga ggcggcgaaa tacgttccgg
     7141 acgaccgcat tttagtggaa accgatgcgc cgttcctcgc ccccgtaccc aagcgcggca
     7201 ggcagaacga accggctttc gtgcgttata ctgccgaaca tatcgccaaa ttgcggaacc
     7261 aaaccttgga acaggttgcg gcatacacga cggaaaactt ttaccggctg tttcaaaaag
     7321 tacccgatat gcggaccgtc tgaccctgta ccgacgataa ggaaaaccat gaaggcaatt
     7381 catccgtatg catgtccgcg ctgctgccgg ctgcctgcca acacgtttcg gacaggcatg
     7441 gcaaattccg cttccaaatt ctgcattgcc aaaggcggca gacgggaagt aaaaaaagac
     7501 gaaagcggcg gcggatatgc cctgtgccat ttgccggaca gcaggattgt cgaggagtgg
     7561 gaatatttcc gttcacaaca ttgatactgc gcgatatacg gcaaatattg tgggaagttt
     7621 ccgcttttgc gtataatgcg ccctacctga caaattttgt caactttatc aaaaggaata
     7681 agtgatggct tccatccacg accaaattaa agaagtagta acgacacacc gcgtcgtatt
     7741 gtttatgaaa ggtacgaagc agtttccgca atgcggtttc tcttcccgcg ccgtgcaaat
     7801 cctgaacgcg gcgggctgca ccgattacgt taccgtcaac gtattggaaa atcccgaagt
     7861 acgccaaggc attaaggaat acagcgactg gccgaccatc ccccaacttt atgtgaacgg
     7921 cgagtttgtc ggcggttcgg acatcctgat ggaaatgtat gaggcaggcg agctgcaaga
     7981 gctgctgaaa gcctgatgga ttcggcaatg ccgtctgaac gtgtttcaga cggcattttc
     8041 ttttccggca aatcaaaaaa aagtataatg gcgcgtctca aaatcacact ggaacaccgc
     8101 gatgaacgtt aatgttatca accatccgct cgtccgccac aaattaaccc tgatgaggga
     8161 ggcggattgc agcacctaca aattccggac gcttgccacc gagctggcgc gcctgatggc
     8221 atacgaggca agccgtgatt ttgaaatcga aaaatacctt atcgacggat ggtgcggtca
     8281 gattgaaggc gatcgcatca agggcaaaac attgaccgtc gttcccatcc tgcgtgcagg
     8341 tttgggtatg cttgacggcg tactcgacct gattccgact gccaaaatca gcgtagtcgg
     8401 actgcagcgc gacgaggaaa cgctgaaacc cgtttcctac ttcgagaaat tcgtggacag
     8461 catggacgaa cgtcctgccc tgattatcga cccgatgctg gcgacgggcg gttcgatggt
     8521 tgccaccatc gaccttttga aagccaaggg ctgcaaaaac atcaaggcac tggtgctggt
     8581 tgccgcgccc gagggtgtga aggcggtcaa cgacgcgcat cccgacgtta cgatttacac
     8641 cgccgcgctc gacagccact tgaacgagaa cggctacatc atccccggct tgggcgatgc
     8701 gggcgacaag attttcggca cacgctaact gactgatttt cggatttgat atgaatttcc
     8761 aagactatct cgccacattt tcttcaatcg accatctggg cggtttggac gttcaggatg
     8821 ccgacggcaa aacggttcac cacattcccg ccgttcaggg taagctcggt tcgctcaagc
     8881 tgtacaatgc tttggcagaa cgttttgacg gaaaattgga caaagaagcg gcagaacagg
     8941 gtttgatatg gtttgccgaa cacgttgccg acgcgcgcgc ccatcccggc aagcatccca
     9001 atatcgacct gcttgaaaaa gttgtgcaaa gcggcgaaac cttgctgctc aagccgcttg
     9061 ccgcgcaata attttcgacc atgccgtctg aaatccgttt cagacggcat tttgtcggaa
     9121 agaagaccgt aaaacgggca ttttcttttc tatttcagga tacgggcaat gatgtttcaa
     9181 cacacaggac ga
//
LOCUS       DAWFBO010000079         9077 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFBO010000079 DAWFBO010000000
VERSION     DAWFBO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.079"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            214..993
                     /gene="pgeF"
                     /locus_tag="WGO87_001296"
     CDS             214..993
                     /gene="pgeF"
                     /locus_tag="WGO87_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0392653.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1045..1524
                     /locus_tag="WGO87_001297"
     CDS             1045..1524
                     /locus_tag="WGO87_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392654.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1526..2524
                     /locus_tag="WGO87_001298"
     CDS             1526..2524
                     /locus_tag="WGO87_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0392655.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSVQSVRNSLR
                     LWGEKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2645..3064
                     /locus_tag="WGO87_001299"
     CDS             2645..3064
                     /locus_tag="WGO87_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392656.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3140..3655
                     /locus_tag="WGO87_001300"
     CDS             3140..3655
                     /locus_tag="WGO87_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0392657.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAVGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3739..4623)
                     /locus_tag="WGO87_001301"
     CDS             complement(3739..4623)
                     /locus_tag="WGO87_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0392658.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLESGIREKISSHLARGKVECKIQIQDTENGNQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTDALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIGALSEIFPALVEAHKEKIRARLAEAVGSIDNDRLQQEFALFVQKS
                     DVDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            4902..5048
                     /locus_tag="WGO87_001302"
     CDS             4902..5048
                     /locus_tag="WGO87_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392659.1"
                     /translation="MLIHYINSANLSEKQLMRQCLVHVCFLISAIAMVWTDKIVYGTT
                     HKPH"
     gene            complement(5107..5970)
                     /gene="rpoH"
                     /locus_tag="WGO87_001303"
     CDS             complement(5107..5970)
                     /gene="rpoH"
                     /locus_tag="WGO87_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0392660.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6234..7772)
                     /gene="lnt"
                     /locus_tag="WGO87_001304"
     CDS             complement(6234..7772)
                     /gene="lnt"
                     /locus_tag="WGO87_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0392661.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(7841..8209)
                     /locus_tag="WGO87_001305"
     CDS             complement(7841..8209)
                     /locus_tag="WGO87_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0392662.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(8109..8567)
                     /locus_tag="WGO87_001306"
                     /pseudo
     CDS             complement(8109..8567)
                     /locus_tag="WGO87_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8763..8999
                     /locus_tag="WGO87_001307"
                     /pseudo
     CDS             8763..8999
                     /locus_tag="WGO87_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
BASE COUNT         1992 a         2261 c         2674 g         2150 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatat aaagctcgga gaaggtatat aaggacatca
       61 ttcctgcacc ggcaagaatc cgaacccgaa cgtttgaaaa caatcccgaa tctccgaatt
      121 cccgcctgtg cgggaatgac gaaaaaacaa gcatccattt gccccgaagg cagttaatca
      181 accctttccg ccacacacct attccaatat ccaatgaaaa ccatcacaga aaccctaaat
      241 ctcgccccga aaggcaaaaa cttcctgacc gccgattggc ccgcgcccgc caatgtgaaa
      301 accctgatta ccacgcgcaa cggcggcgtg agccaaggtg cgtatcagag tttgaacctc
      361 ggtacgcacg tcggcgacaa tcccgaagcc gtgcgccgca accgcgaaat cgtgcaacag
      421 caggtcggat tgcccgttgc ctacctcaat caaatccaca gtaccgtcgt cgtcaatgct
      481 gccgaagctt tgggaggcac acccgatgcg gacgcttccg tagacgacac gggcaaagtt
      541 gcctgtgctg tcatgactgc cgactgtctg cccgttctat tttgcgacag ggcgggtacg
      601 gcggttgccg ccgcacacgc gggctggcgc ggtttggcgg gcggcgtatt gcaaaacacc
      661 attgccgcga tgaaggttcc acccgtcgaa atgatggcgt atctcggtcc cgccatcagt
      721 gcggatgcgt ttgaagtcgg acaggatgtg tttgatgcgt tctgcacgcc catgcccgaa
      781 gccgccgccg catttgaagg cataggcggc ggcaaattcc ttgccgacct ttacgcgctc
      841 gcccgcctga ttctgaagcg cgaaggcgtg ggcggcgtat atggcggcac gcattgtacg
      901 gttttggaac gggatacttt cttttcctac cgccgcgacg gagcgacagg gcgtatggcg
      961 agcctgattt ggctggacgg caatgccgtc tgaacacgcc gctgatataa tctaccgact
     1021 ttgtgctttt gagaaaggca agccatgaac aaactgtttc ttactgccgc agtgctgatg
     1081 ctgggcgcgt gcggtttcca cctgaaaggt gcagacggca tttctccgcc gctgacctac
     1141 cggagctggc acatcgaagg cggacaggca ttgcagtttc ctttggaaac cgcgctgtat
     1201 caggcttcgg gcagggtgga cgatgctgcc ggcgcgcaga tgaccctgcg tatagacagc
     1261 gtttcccaaa acaaggaaac ctacaccgtt acccgtgcgg cagttatcaa cgaatatctt
     1321 ttaatcttga cggttgaagc gcaggtattg aaacgcggcg agccggtcgg caaaccgatg
     1381 accgtgtccg tccgccgcgt ccttgcttat gccgacaacg agatcttggg caaacaggaa
     1441 gaggaagcgg cattgtgggc ggaaatgcgg caggatgccg ccgaacagat tgtccgccgc
     1501 ctgacctttc tgaaggcgga atgacgtggc ggcacatatc ggacacattg atacggacgc
     1561 gcctttgaaa cccctgtacg tcatccacgg cgaggaagaa ctgttgcgta tcgaggcatt
     1621 ggacgcattg agggcggcgg cgaagaaaca aggttacctt aatcgggaag tttatacggc
     1681 agacaatgcc ttcgattgga acgaactgtt gcaaaccgca ggcagtgcgg gtctgtttgc
     1741 cgatttgaag ctgttggaac tgcatatccc caacggaaaa cccggcaaaa ccggcggcga
     1801 ggcgttgcag gattttgccg cccgcctgcc ggaagatacg gtaacgctgg ttttgctgcc
     1861 caaactggag aaaacccagc tccagtccaa atggtttgcc gcattggcgg caaaggggga
     1921 agtgtgggaa gccaaaccgg tcggcgcggc ggctttgccc caatggatac gcggacggct
     1981 ggacaaaatc ggtttgggta tcggggcaga cgcattggca ctgtttgccg agcgcgtgga
     2041 aggcaatctg ttggcggcgc gtcaggaaat cgacaagctc gggctgctgt atccgaaagg
     2101 gcataccgtc aatatcgatg aggcgcaaac cgccgttgcc aacgtcgccc gcttcgacgc
     2161 gttccaactg gcaggcgcgt ggatgaaggg cgatgtcctg cgcgtatgca ggcttttgga
     2221 cggattgcgg gaagagggcg aagaaccggt gctgttgctg tgggcggttg ccgaagacgt
     2281 gcggacgctg atccggcttg ctgccgccct gaagcagggg cagagtgtcc aatccgtacg
     2341 caacagtctc agactttggg gtgaaaagca gacgctcgca ccgcttgcag tcaagcggat
     2401 ttccgtcgtc cgcctgcttg acgcgctcaa aacctgcgcc caaatcgacc gaatcatcaa
     2461 aggtgcggaa gacggcgacg catggacggt attcaaacgg cttgtcgtgt cgctggcgga
     2521 ataaagcggt aatccccaaa atccgaaaat actgtaaaat accgttaatc ctgaaaagta
     2581 ttcaccaatc cgtccgaaaa catttcagac ggcacgacca cctcaataaa ggaacattaa
     2641 ccctatggac aataagacca aactgcgctt gggcggcctg attttactga ccaccgccgt
     2701 tttaagcctc attatcgtat tgattgtcga ctcctggccg cttgccatcc tgcttgccgc
     2761 cgtcatcgtc gccgccgctg cgggcggttt tgtttggaca tcccgccgac agcaacgcca
     2821 gtttatcgaa cgcctgaaaa aattcgacat cgatcccgaa aaaggcagaa tcaacgaggc
     2881 aaacctgcgc cgtatgtacc acagcggcgg acaacaccag aaagatgcga ttaccctgat
     2941 ctgcctgtcg caaaaatgtt cggtggacga ggcgcacgct atgttcaaaa aacgcccgac
     3001 acgtcaggaa atcaatcaaa tggcggcaaa acagtcgcgc ggtcagaaac gtccgcaccg
     3061 ttaaccgccg caaggcatct ttgcataaat gccgtctgaa gcctgttggc gtttcagacg
     3121 gcatattctg attgaaaaga tgatgacact gaaaaccgcc ccgctcaaac gccgctttgc
     3181 cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag cattgtttgc
     3241 cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat tggtaacgag
     3301 tatcctgata atgggatcat ggtggcttta ttttcgcgcc aactggcacg gtcaggggca
     3361 gaccttggcg atgaggacat ggaaaatcgg cttgtgcgac cttaacggca tacagccgtc
     3421 tttgcacctg ctgcgcctgc gctttatttg ggcgtgcata ttcatcgtct tcattcctat
     3481 gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg cggtcggtgc
     3541 ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg atcggcagtt
     3601 tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc cttaagcctt
     3661 tataccgcaa aggtttcaac ctgaaaaaat gccgtctgaa agggctttca gacggcattt
     3721 gcttatcggg gaaaccgatt attcgatatt ctgcacctgt tcgcgcatct gttcgatcag
     3781 gactttcagc tcgaccgagg cttgggtgca ttcggcggca atggatttgc tgcccaaagt
     3841 gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgttttccgc tgctgccttt
     3901 gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgcaggcggc tgaattcttc
     3961 atcgacatcg gatttttgga caaacagggc aaactcttgt tgcaggcggt cgttgtcgat
     4021 gctgccgacg gcttcggcaa ggcgggcgcg gattttttct ttatgcgctt ccactagcgc
     4081 ggggaagatt tcgctcaatg ccccgatgat ttcttccata gactcgaggc gttgcagcag
     4141 gtgttcgccc aattttttcc cttcgcgtcg gcgtgcggcg gtaaagtctt tcagtgccga
     4201 atcggtcaaa tcggcaataa cctttgccaa tgcgtccgta ttttcccttt ggcttgccaa
     4261 tacgccgggg aaacgcagga tgtcggcaac gcccagtttt gccaaatcgt gatgcttgcg
     4321 gaggtctttg ttgatttcgg caagctgtcc gaccagatcg cgattcagtt ccaaagactg
     4381 attgccgttt tccgtatctt gaatttggat tttgcattcg actttgccgc gtgccagatg
     4441 ggatgaaatt ttctcgcgga taccgctttc caaatggcgc aaatcatccg gcatcctgat
     4501 ttgaatgtcc aaaaagcggt ggttgacggc gcggatgtcg aggttgatgc gtttgctgcc
     4561 gcattccgcc gccgcgttgg caaagccggt catgctgtgg atgtggatat ttccgctgct
     4621 catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt atggcggcaa
     4681 gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc acgcgctttt
     4741 gttgctgcaa ggaagggaaa tgtatagtgg attaaccaaa accagtacgg cgttgcctcg
     4801 ccttagctca aagagaacga ttctctaagg tgttgaagca ccaagtgaat cggttccgta
     4861 cgatttgtac tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc cactatatca
     4921 attccgccaa tctgtcggaa aagcagctga tgcggcagtg tctggtgcat gtctgctttt
     4981 tgatttcggc gattgcgatg gtttggacgg ataaaatcgt gtacggcacg acgcacaaac
     5041 cgcattgatg tttaccaaat aaaatacccg acaaaacaat ttgtcgggta ttttattgcg
     5101 tatatttcaa accgcttcgg cttcttcggt caggaaacca cgcagtttct gcatggcttt
     5161 tgcttcgatt tggcggatgc gttcggcaga tacgccgtat tcggcggcaa gctggtgcag
     5221 cgtcagcccg ccgtcgtctt gaagccagcg gctttccaca atacggcggc tcctgtcatc
     5281 cagttgcgcc aaagcgtttt gtaaaccttc tgtttgcagg gcgtaatgcg cctgtttcga
     5341 tagttgtcgg ctcggttcgg aatcgtggtc ggcaagccag tcgatggggg cgaaactgtc
     5401 ctcgtcgtcg ctgttgtctg ccatgatggc gatgtcgtgt cccgtcatgc gctgttccat
     5461 ttccagaact tcggaaagtt tgacacccaa atcgtcggcg atgtcttgtg cctctttggg
     5521 agacagggcg ttgaggtttt tacgcatact gcgtaaattg aaaaacagct tgcgttgcgg
     5581 tttggtggtg gcaacgcgga caaggcgcca gttcctcagg atgaattcgt gaatttccgc
     5641 tttaatccaa tgcacggcaa atgaaaacag gcgtgcacct ctaccgggct cgtagcgttt
     5701 gaccgccttc atcagtccga tattgccttc ctgaatcagg tctgcctgat tcagcccgta
     5761 gccgtcatag ccgcgcgcga tggaaacgac gacgcgcagg tgggacagga tgagttgttt
     5821 ggcggcgttg aggtcgccct ttatccggcg ttcggcgagg cgggtttctt cttcctggga
     5881 cagcatcggg atgctgttga cggtgtggat gtattgttcg aggctgccgt tgccgctttg
     5941 gatggcgggt aatgcgaaag cgttattcat ttggggcatt tcctttcggc tgaaactgcg
     6001 tatcggcggt ttgctgtgtt gggatgcagt atatcactgc ttggcttgta ttttgtatgt
     6061 ttggcaggag atatgcgcta aggtttgaaa gacaggaaaa ttttgtaagg caggtttgat
     6121 tgattttgta aacctgatgg ctcaatccga tttcggaatt atattacata cgtagttgta
     6181 tgtaaacagc cggtttgaaa aaagacagcc cgtccggacg ggctgtgcag gtatcagtgt
     6241 tctttgtttc ggaagatgaa aagaatcagt gcggctaggg tcaatatgcc catcaaccac
     6301 catgaactgc cggttttcat atagggtgtt tcgccgacat agcctttgat gtgtccttcc
     6361 aatacggttt ccgtatcggg ttgggcttgg gcgatgatgt tgcctttggg ggagatgatg
     6421 gcggttgcgc cggtgttggt ggcacggacc atatagcgtc cgagttccat agcccgtgcc
     6481 tgcgattgtt ggaggtgctg gtacatggcg ttggattttc cgtaccacgc catattgctg
     6541 gcattggcaa gcagggtggc atctttggcg gcggcaatca gttcgtcgcc gaatccgtct
     6601 tcgtaacaga tgttgaaggc gattttttgg tttttcatca gcagggcgga ttgcttaccg
     6661 ccgcctttgc ggaagtcgga aaggggcata ttcatcattt tgtaaagcgg cgtggtcagg
     6721 aagggcagcg gtttgtattc gccgaagggg acgaggtggt ttttggcgta gtaggggatg
     6781 ccgtcctgat tgttttcctg ataaccggtc aggttgatga cggcgttttc gtaaccgttg
     6841 ccgtccgaag tgtattggct gatgccgacg gcgagcgcgc tgccgttgtt ttgcgcctgt
     6901 tcggcaaatt ttgccagtat gttttccggc aggttttggc gcataacggg gatggcggtt
     6961 tcgggcagga tgacgatgtc ggcagtggtt ttgccgacct gttcgtaata tttctgtatg
     7021 gtcgggatga cttggtcttc acgccatttg agggtttggt cgatgttgcc ttgaagcagg
     7081 gcgacggtgc tgcggctgcc gtcggggcgg gtgaagtcgg tttgtcgggc ggtgtagcct
     7141 gcggcaagca gggcggcaat caggataatt ggaagcaggc gtttgcccga acgtgcggtg
     7201 ttatcactcg ccaaaaccag ccagacaccg agaaaggcgg ttgccagtgt aaccatgtgg
     7261 atgccgccca atggggcaaa gccggcgagc gggctgtccg gggtgatttg ggagtagccg
     7321 attgcgcccc agccgaatcc ggtcaggaaa cgttcgcggg caaactcggt cagtgtccac
     7381 aggatgggca gtaccaaacc gatttttatg ccccgaggca gggtaaattt tttccacagc
     7441 cagaaacaca gtgccggata aagggcaagg taggcgggga gtaggaaggt cagcggtacg
     7501 gcatagaggt cgggcaggcc ggaaacgtcg tgcagggcgg tgtgtatcca gtagaactgt
     7561 gtcgtgtatg cggtcaggcc gaacaggtag gcggaagaga cagcaaaacg cggacgcagt
     7621 tcgatgaggc ggacgaaggc accgaaaatc aagggcatca gccaaaagtg gtagtagggt
     7681 gcgaaggtaa agggggtggc ggcggcaaaa aggatgagca aaggccagta gagggcgggg
     7741 tgctgccagt atttgtccag tttggaaacc atattcatct gtctgttcgg aagataccgt
     7801 ctgaacatct ttcaaacggc atcggtattt gaaaaagaaa tcaatgcctg ccgaaacgat
     7861 tcatcagcgg caaggcgggg gcgcaggcaa tcgaaccgga aagcagcccg acaacgaggt
     7921 tggcgaagta ctccgcccag ccagcgtccc agtgttgcgc gtgcaggaaa tcgtgcagaa
     7981 ggccgaggtt gtgggcgacc agtacgccgc cgataagaaa catggcaagc gtgccgacca
     8041 cgctcaaacc gcgcataaag caaggcataa aggcagtcag catttgcccc aaactgcgcg
     8101 aaaaggtttg tgggcggcgc atcagcagca tgcctaagtc gtcgagtttg acgatgacgg
     8161 caacgattcc gtacaccaaa acagtcatgc cgatgccgat tgccgccatt acgagcatgc
     8221 gcgtcatgtc ggtgcagaaa cttgtgcagc agcttttcta cgccttcaaa gcacagataa
     8281 atgccgcctg ccgtcaaaag cggcgtaatg agttgcggca ggaaggcgga aagcagcagg
     8341 gccgcaggca ccaaaaccgg cttgttggaa aaagaacctt tcgccatcga ccaaacaatc
     8401 ggcaactcgc gttctgccga tacgcccgta acacggttgg cattgggtgc caaatcgtcg
     8461 ccgaccacgc cggcggtttt ctttgcggcg gctttggtca tcagggcaac atcgtccaaa
     8521 acggcggtga tgtcgtccgg cagggtaaat agtgaggcaa atgccattaa agaatcctga
     8581 aatgcggcgc aaagtccgac attatatagg agaacgcgga tttgggcggt ttcaggcggc
     8641 atgaaacagg aaaatgccgt ctgaacgctg tggcggacgt gaagtaaagt ttcgtgaaaa
     8701 gaaaatgccg tgttacagtc tttcgatttt aatttaatga attttaaggg agaatcgtta
     8761 gcgtggattg gatgggcagt ctgttcctgc cgggtggcgc actgttgttt ctgagcgtgg
     8821 tttcgaccac tttacccgca cgtttgggaa tgcctttgct gctggtttct cccgccggcg
     8881 tgttggacgg ggcggcggaa gccttggcga ttgcggcgtt cctgatgctg gtcgcgcgtc
     8941 cgccggcagt gttcggcggt ttgtggaaat tcaattacag cctgcgtgaa aaggcgtata
     9001 gccgaatcga aatgcagtcc gacaccgtgc ttcaggcggg gatttggcgt ggtacatcct
     9061 gcccgacggc aaggtcg
//
LOCUS       DAWFBO010000080         8980 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFBO010000080 DAWFBO010000000
VERSION     DAWFBO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.080"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(100..297)
                     /locus_tag="WGO87_001308"
     CDS             complement(100..297)
                     /locus_tag="WGO87_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392663.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWSITFLNHPF"
     gene            complement(294..1349)
                     /locus_tag="WGO87_001309"
     CDS             complement(294..1349)
                     /locus_tag="WGO87_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0392664.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1455..1937)
                     /locus_tag="WGO87_001310"
     CDS             complement(1455..1937)
                     /locus_tag="WGO87_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0392665.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1984..3120)
                     /locus_tag="WGO87_001311"
     CDS             complement(1984..3120)
                     /locus_tag="WGO87_001311"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0392666.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKSWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGKPDWFPLQS
                     RPDARLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            complement(3117..3662)
                     /locus_tag="WGO87_001312"
     CDS             complement(3117..3662)
                     /locus_tag="WGO87_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0392667.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKNREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTHQTGIRANT
                     QTDFSKFKGCLKDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3659..5134)
                     /locus_tag="WGO87_001313"
     CDS             complement(3659..5134)
                     /locus_tag="WGO87_001313"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0392668.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTSDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERNDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSNPESEWQETQNKARAVIHAAQMVQEGLDK"
     gene            complement(5193..5354)
                     /locus_tag="WGO87_001314"
     CDS             complement(5193..5354)
                     /locus_tag="WGO87_001314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392669.1"
                     /translation="MPSEALPAGKRARSLKPPPAKCPLRVPDGHLKTRRAASRTLQTA
                     SRSDCHQFR"
     gene            complement(5344..7254)
                     /locus_tag="WGO87_001315"
     CDS             complement(5344..7254)
                     /locus_tag="WGO87_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0392670.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVVLAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSL
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTASAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPEIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7273..7917)
                     /locus_tag="WGO87_001316"
     CDS             complement(7273..7917)
                     /locus_tag="WGO87_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0392671.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8081..8899)
                     /gene="opc"
                     /locus_tag="WGO87_001317"
                     /gene_synonym="opcA"
     CDS             complement(8081..8899)
                     /gene="opc"
                     /locus_tag="WGO87_001317"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ0392672.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         1920 a         1974 c         2573 g         2513 t
ORIGIN      
        1 cgctgtactg gtttttgtta atccactata aatgttccga tacgaactgc aaaatattgg
       61 ttttgtttct gacaggcaaa agcactgttt atttggctgt caaaagggat ggttaaggaa
      121 agttatgctc cagataagga tggttgcgcc gacggcttca gacggcattt tggcggcggt
      181 gttgggtttt gtatccggtt tgccgttgtg tcttgtgatg atgattttgg gcgcggtttt
      241 tctgttttga tgtgtgaaat gccgtctgaa aggcggttca gacggcatag cggtcatttt
      301 tgtgcggtca ggcggtcgaa tatgccgccg tcggcgaagt aggttttcat gatgttgtcc
      361 catccgccga atttttcttc gggagagaat gagtctaagt ctgggaagtc ggctttgtgt
      421 cttgccaata cttcggggtt gcgggggcgc aggtagagtg aggcggcgag ttcttgcgcc
      481 ggttcgctcc aaaggtattc gagataggcg cgggcggttt tttgcgtgcc ttttttcgcg
      541 acgacgctgt tgacgacggc gacggggctt tcggcggaaa tggtgtagct cggatagacg
      601 atttcaaact gtccttgggt cagttttttg ctgacgtagt tggcttcgtt ttcaaaagtg
      661 atgagtacgt cgccgatgtt gcgttgtgtg aaggtggtgg tggcggcgcg tccgccgttt
      721 tcaaaaacgg gggtgttttt gaggatggat gcgacgagtt tttgggcttc ctgttcgttg
      781 ccgttggtgg ttttcagacc gtaaccgtat gcgccgagga aggcgtagcg tccgttgccc
      841 gaggttttgg gattggcgat gacgatgtta acgccgtctt tggcaaggtc gttccaatcg
      901 cggatctgtt tggggttgtt ttttcggaca aggaaaacca tagtgctggt gtagggcgcg
      961 gcgtggtcgg ggagggcttg ttgccagcct ttttctacca gtcctttttt ttcgagcagg
     1021 tcgatgtcgg aggattggtt catggttacg acatcggctt gaaggccgtt ggctacggat
     1081 aatgcctgtt tgctggagcc gccgtgggac tgttggatgc tgacggatgt gccggggtgt
     1141 tcggattggt atgttttgat aaataagggg ttgtattctt tgtaaaaatc ccgtgccaca
     1201 tcgtatgagg cgttgagcag ggtaatgttt tttccgtcgg attcggtatt ggccggggca
     1261 ttttgtccgg acggatgatt tgaatcggct gcggggctgc aggcggtgag cagggctgcg
     1321 gtatagagtg ccggtgcgta ggttttcata tgcttgtcct gtcggttggt agatggggca
     1381 actttatacg gctgtctgcg cttgtggaaa taatgtttga tttgaagatt atcagttttg
     1441 gttataagga cggatcagag gtgtttccgc atcagttcgc atttgatttt gatgctgggg
     1501 tcaagctgca atactgccga accgagcgat tcgtagcggt tgagaaggta aaacgggacg
     1561 gagttgagcg atgcgtagag tttcagaaag ctcaaaccgg atttgtgggc gatggtttcc
     1621 gcctgatgaa gtagggcagt gcccagtccg aggttgtgga acagggggtg gacgtaaagc
     1681 gcatcgagtt gtgcttcttg gcagtcgatt tggaaaaatc cctgtatgtt gcctttgtat
     1741 tcggcaaccc aaagtgcttt gtcgggatcg gaaatggtcg gcaggtagct ttctgtgttt
     1801 agcaagcctt cccatacttt tagggcgtgt tcgttgtagc tgaggatgca ggtgtattgg
     1861 acggagtgca ggtggacttt gaagatgtct ttgcagtctt gcacggtggc ggggcgtaac
     1921 agtgtcagca ggttcatggc agtatgtcgg cggcttcaga cggcatctgt gccgttggtc
     1981 ggattatagg gactgatgca gtttttttgc ttcttgaaat gcggtgtccg aatcggtggt
     2041 taaaacggta aagtgtccca ttttccgccc tttgtgcgcg gtttttttgc cgtaaaggtg
     2101 caggcgcgcg tccggccggc tttgcagggg aaaccaatcc ggtttgccgc cgtcttcctg
     2161 ccaaacgtcg cccaaaatat tcgccataca gcaagaactc agcaatttgg tgtcagcagg
     2221 tggcaggttg cacatcaggc ggacctgttg ctggaattgg tctgccgcgc aggcatcgat
     2281 ggtatggtgg ccggagttgt gcggacgcgg cgcgatttcg ttgacgacca attcatgcgt
     2341 gtcgccgaca acaaacattt ctaccgccaa tacgccgacg tagttcaatt catcggccaa
     2401 acgctgcgcc atttgtcgcg cctgttgctg aatgtcggca ctcagtcggg ctgggacgat
     2461 ggagtaggcg aggataccgt tttcgtgaat gttttcggca ggatcgaaag tttgcacgtt
     2521 gtcattgttc agacggcata cgataacgga aatttcgccg cgcaagtcca ccattttttc
     2581 caaaacgcaa tccacgccgc ggtgttcggc aaacgcggct ttgagttcat ccaccgtttt
     2641 gacgcggatt tggcctttgc cgtcatagcc caatgtagcg gttttcagga tgccgggcag
     2701 aaattgtatg ctttcttcag tgatgtcttc ggctttgcaa attgcttgat acggcgcggt
     2761 ttgcaggcct gctttgcgta tccatgactt ttcctgaatg cggttttgcg cgatggcaac
     2821 gcagtcgccg ctgggggaaa cattggtgtg tttggcgaga aaacgcatcg catcggcatt
     2881 gacgttttca aattcagtgg tcaccgccgc gcattttgcc aattcgtcca aagcagcttg
     2941 gtcgttaaac ggcgcgcata aatgacagtc ggcaaattct gctgccggcg cgtctggatc
     3001 ggggtcgaga acggtgactt tgtagcccat ggttttggcg gcaacggtaa acattctgcc
     3061 taattgtccg ccgccaagaa tgccgagcat ggcgggcgga gaaagagata tgtttttcat
     3121 gctgactctt caaattgtac aagttgatag ctataactaa ttcttgacgg atgtcttgta
     3181 tcgctggaat taccagtttc agaaatacag aatacttttt ccataaattt ttctgctttt
     3241 agaaattcca gtattctgtt tttttcatcc ttataagcac cgcggtctgt accccatgca
     3301 agaataatca tatcggcatc tttcaaacat cctttaaatt tggaaaaatc ggtttgggtg
     3361 tttgccctaa ttcctgtttg atgtgtagag taactggaaa aaatatttaa cattttgaag
     3421 ttggtaaaac cgtacatatc caagaaacgt gcaagttggg taagggtttt gtcgcttctt
     3481 tcatcatttg ctttacttgg attaattcct atagcgacag ctgagaaatt tttaggattt
     3541 tctgtgttgc cgctccatct aaccgttagg atttctcgat ttttttcatt atctgtatag
     3601 agaccgtctt tggtagtcgg tctgagtgtt tggcgaagct cataaagttt ttcataagtc
     3661 atttatccaa cccttcctgt accatttgcg cggcgtgtat gacggcacgg gctttgttct
     3721 gcgtttcctg ccattctgat tcaggattgg aatcggcaac cacgcccgcg ccgctttgga
     3781 cgtatagtgt gttgtttttt actacggcgg tgcggatggc gattgccaaa tccatgtcgt
     3841 tgttgaaacc ccatacgccg acggcaccgc cgtagatgcc gcgtttgctc ggttcgactt
     3901 cttcgatgat ttccatggcg cggactttgg gtgcgccgga gagtgtgccg gcagggaagg
     3961 tagcggcgag gatgtccatg ttggtcatgc cgtctttcag acggccttcg acgttggaaa
     4021 cgatgtgcat tacatgggag tatttttcaa tcaccatttt gtcggtaact ttgacttcgc
     4081 cggttttact gatgcggccg acgtcgttgc gtcctaagtc aatcaacatg acgtgttcgg
     4141 cgatttcttt ggcatcgctt aacaaatctt gttcgttggc aaggtcttcg gcgggggttt
     4201 tgccgcgcag gcgcgtgccg gcgatggggc ggacgataac gtcgttgcgt tcgcgtcgga
     4261 cgaggatttc gggcgaggag ccgacgatgt ggaaatcgcc gaaatcgtag taaaagagat
     4321 aaggcgaagg gttgagcgta cgcagggcgc ggtagagggc gagcgggctg tcggtgaatt
     4381 ccatgctcat gcgctggctg gggacaacct gcatgcagtc gcctgcaaag atgtagtctt
     4441 tgattttgtt aacgcaggct ttgaacggct cttcgccgaa ttcgctgacg gcttcggtgt
     4501 gtttgctgcc gagcgagagc gggatggcgc agctttggcg caactgggtg cggatgtctt
     4561 cgaggcgttc gcgggcgcgt tcgtagccgt cgggctgcga cggatcggcg taaacgacga
     4621 gatggatttt gccgctcaaa ttgtcgatca ccgccaattc ttgcgacagc atcagcaaga
     4681 tgtcggacgt gccgagcggg tcggctttgg tggtgttttt caggcggtgg gcgaagtgtt
     4741 cgaaattgta gatggtttcg taaccgaagt agccgaccag tccgccggta aagcgcggca
     4801 ggcttgggat ttcgggtgtt ttgaagcggt tgtggaaggc ttcgataaag ggcagggggt
     4861 tgccgtcgtg ttgctcgaca atttcgccgt tttgataaac atcgacgtgt ttgccgctgg
     4921 ctttgagata gtggctgcaa ggcaggccga taaaagaata gcggccgaaa cgttcgccac
     4981 cgacaacgga ttcgagcagg taggtatagg ggcggttggc gagtttgaga tagagggaaa
     5041 gcggcgtatc caagtcggca aggagttctt gcacgagcgg gatgcggttg tagccttggg
     5101 cggcttgggc ttggtattct tgtttgctga tcatttctgc tttcccaaaa ggcggtttcg
     5161 gacggcgcgg taatgggcgc gagtatagca ttttatcgga attggtgaca gtctgaccga
     5221 gaggccgtct gaagggtgcg gcttgcggcg cggcgtgttt ttaaatgccc gtcaggtacg
     5281 cggagcgggc attttgcagg cggcggcttc aggcttcggg cgcgttttcc cgccggcagg
     5341 gcttcagacg gcattgccgt tttgtttggt ttccagcagc tcccagcgtt ccagcttttc
     5401 caaaagcagc atttcgattt cttcggcgcg gttttgcaat gcgccggctt tttcgtaatc
     5461 tttgaaaatt tcaggatcgg aaagttgcgc gttgatttct gcctgttcgg tttccaaggc
     5521 ggcgatttca tcgggtaggg catcaagttc gcgctgctct ttgtaggaca gtttgaccgt
     5581 gcggttggct ttgggttttt ctttcaccgg ttcggcatcg gctgttttgg gtgcggatgc
     5641 cgtctgaatc ttcgcttcgc gtgattttgc gtcgatgtag tcctgatagc cgccgatata
     5701 ttcttttaac cttccatttc cttcaaaaac aaggctttgg gtaatcacgt tatcgaggaa
     5761 catacggtcg tgcgagacaa ggaataccgt gccttgataa tcgcgcaaca ggtcttcgag
     5821 cagctcttgg gtgtcgatgt ccaagtcatt ggtcggttcg tccaagacca aaatattggc
     5881 gggacgggta aagagttttg ccagcaaaag gcggttgcgt tcgccgccgg agagtgaaga
     5941 aacaggactt tgagcgcggg caggggggaa caggaaatct tccaaatagc tcatcacatg
     6001 ctttttctta ccgccgactt caacgtaatc attgccctgt ccgagcgtgt aaaacacggt
     6061 gtcgttttca ttcaacgcgc tgcggaactg gtcgaaatag gcgacttcct gcttgctgcc
     6121 gatacggatt ctgccgtagg tcggctgcaa ttcgcccaaa atcagtttta aaaatgtggt
     6181 tttgccgatg ccgtttggac cgattaagcc gattttgtcg ccgcgctgca agatggcgga
     6241 gaatttgtcc ataatgactt tgtcgccata agcaaacgag gcgtgttcca gctcggcgat
     6301 gattttgccg cttttctcgc cgctgtcgag cttgaaattg acctgccctt gtacgttgcg
     6361 gcgttcggca cgctggcggc gcagttcttc caaacggcgc acgcggcctt cgttgcgggt
     6421 acggcgcgct tcgatgcctt tgcgtatcca cgcttcttcc tgcgcgtgga atttgtcgaa
     6481 gaggcggttg tgttccgctt cgactgccaa ctcttgcgct tttttctcgc tatatttgga
     6541 gaacgagccg ggataggaac gcaaaatacc gcgatcgagt tcgacaatcc gcgtggcgat
     6601 attgtccaaa aaacggcggt cgtgggtaat cacaaccaag ctgccttcaa acgctttgag
     6661 cagattttcc agccaaataa tcgcgtcgat atccaaatgg ttggtcggct cgtccagcag
     6721 caatacgtcg ggcttttgca cccaagcctg cgccaaaacg acgcgctttt tctgaccgcc
     6781 ggagaggttg ccgatttttt cgttttccgg caaaccgagt tcccccaaag tttgcttgac
     6841 tgccgcatcc agtttccagc cgtccttcgc ttcgatttca agttgcaatt cgttgagctc
     6901 tttcaacaaa gcctcactcg aaccattttc caactcatgg ctaacatgat gataacggcg
     6961 caataaatcg cgaatttcgc ctaaaccttc ggcaacggta tcaaatacgg ttgcgtcctt
     7021 atcaaaaaag gattcctgcg gtacataaac gattttgagg ttgttttgaa caataatctg
     7081 cccgtcgtcg agcttttgca caccggcgag gatttttaaa aacgaagact tgcctgcgcc
     7141 gttgcgtccg atcaagccga ctttttcgcc gctgtcgagt tgaaaagaag ttttgtcgag
     7201 caaggcaacg tggccgatgg caaaagaagc gttttctaca gataatatat tcatgataca
     7261 aattctcaac agttaccgtt tggattttac cgcaagtttg gcgcgggcaa tttcaaccgc
     7321 acccggcagg acggaaacaa taatgatgcc gcccatcacc aagcccagat tgttttttac
     7381 gacggggaag ttggcaaaga aatagcccgc gtaagaaaac aggataaccc acaacaagcc
     7441 accgatgatg ttgtagcgga taaatttggc atagtgcatt ttccccatac cggcgacaaa
     7501 gggggcgaag gtgcggacga tgggtacaaa acgggcaatg atgatggttt tcccgccgtg
     7561 tttttcgtaa aaacggtggg ttttatcgag atattcgcgt cggaagattt tagaatcggg
     7621 attggcgaac agcctgccgc cgaaatattt gccgacggta aaattgagcg cgtcgccgag
     7681 tatggcggca aggcttaata atgcaaccat caaatgaata tccataccgc ccagcgcggc
     7741 aatcccgccg gcggcaaaca gcagcgaatc gccgggcagt aagggcgtaa caatcaggcc
     7801 ggtttcgcaa aaaacaatca aaaacagaat cgcataaatc cacacaccgt attgcgccga
     7861 cagcgcaagc aggtgttggt cgatatggag gatgaagtcg atggcggagg caagcacggc
     7921 gcgttcctaa aaaaaacaaa ccgcgtattt taaccgattg gaaaaatgcc gtctgaaatt
     7981 ttcagacggc atcggctatt caaattcatt tcacgtaaaa accgcaaacc aaaatagttt
     8041 gcggtttggc atttaaagtg acaatgatga tttcaaatca tcagaatttt atgccgacgc
     8101 gcaagccgta ttcacgaata ctggttttcg ggatggtgag cgatacgtcg ccactcttgg
     8161 ttgttacact aaactcgccg gattctttgt aagtgcgttg tttgtagaac ggccccgcct
     8221 cgatgctggc ggattcgccc agttttttac caatatttgc acccaatcca aagccccagc
     8281 cgttggtttt ttgattgatg tcttttttga gattggtaac gccggtgttt aatttatagc
     8341 gggaattgag gtcaaatttc acttcagacc aagggttgat ataccagccg ttacccagtt
     8401 gggaaagcaa atccgcgtga actttggcta accacgactg acggctgctg tgaagcgtat
     8461 gcttggtggt tttaatgctg tcttttgaag attcaaaacc caagccggca cccacacgga
     8521 aatttaaaga atcacttaac gtttgggtgt aggtgtagcc tgtgtaaaga tcgatacggt
     8581 tttcaggaac gccggtgggc agttttacat ttttagtctt acccagcttg ttctcatctg
     8641 tttccaaatt aataatattt tttttgctgc gcccgaaacc ggcttccaag cggatgcctt
     8701 ggttggcatc aaaaggaata tcagcacgta cgccgatgtg tttggcagct ttgtgttttt
     8761 ctttcaggaa agcacgagtt gaagaaatgg aagagaggtc ggtgtggacg gtaaactcat
     8821 tagcggtttg aagctcttgt gcagcggcgg cagtaccggt cagggcaatc atggcacatg
     8881 taaaaactgt ttttttcata gttaaaatct ccaaaatttg gattgtagtc ggatatggta
     8941 acataacgta aataatcgtt acgcttacaa ttatattctt
//
LOCUS       DAWFBO010000081         8701 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFBO010000081 DAWFBO010000000
VERSION     DAWFBO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.081"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            191..1726
                     /locus_tag="WGO87_001318"
     CDS             191..1726
                     /locus_tag="WGO87_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0392673.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRRHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="WGO87_001319"
     CDS             1723..1815
                     /locus_tag="WGO87_001319"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0392674.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="WGO87_001320"
     CDS             complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="WGO87_001320"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0392675.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3106..3276)
                     /locus_tag="WGO87_001321"
     CDS             complement(3106..3276)
                     /locus_tag="WGO87_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0392676.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3348..3671
                     /gene="cyaY"
                     /locus_tag="WGO87_001322"
     CDS             3348..3671
                     /gene="cyaY"
                     /locus_tag="WGO87_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0392677.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3698..4714
                     /locus_tag="WGO87_001323"
     CDS             3698..4714
                     /locus_tag="WGO87_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ0392678.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKVSVAIAIV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENINPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4740..5159
                     /locus_tag="WGO87_001324"
     CDS             4740..5159
                     /locus_tag="WGO87_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0392679.1"
                     /translation="MAQLPLYRTAEIENFTVGTPEVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAQGEWPKVTDRVLDDELQDKILSRKPT"
     gene            5181..5687
                     /gene="luxS"
                     /locus_tag="WGO87_001325"
     CDS             5181..5687
                     /gene="luxS"
                     /locus_tag="WGO87_001325"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0392680.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVANVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5833..8628
                     /gene="polA"
                     /locus_tag="WGO87_001326"
     CDS             5833..8628
                     /gene="polA"
                     /locus_tag="WGO87_001326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0392681.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGLESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESRMHEAADGDLFGSDTIGEQAALDMETSSERLPEKAVAPEKLDYQA
                     VTTEAQFATLLDKLSQAEKIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGHLKPYLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         2039 a         2252 c         2295 g         2115 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaggcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttacag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta ttcgttgccg tccaccaaca cctctgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggccccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tccccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcgcaaatc
     2101 ggaccgacga tgttggcggt cagaggctca atgtctttgg tttcgaccgc ctcgatgtga
     2161 tgataggcat cataaagcgc cgggcgcatc aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgta tttgacgaat tcgacgcgtg tcagcagcga acctgcgttg
     2281 ccgaccaagc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaacggctt gggcatacgc gcccaaatca ggcacttttt cgttttggta aacaatgccg
     2401 acgccgccgc ctaagtccaa atgttccaaa acaatgcctt cggcggcaag cgcgtcaacc
     2461 aaaatcaaaa tgcgttcgca ggcttcgacc agcgggctta agtcggttag ttgggagccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg gcggtaggct
     2581 tcgagcgcgt cggcgtaggc gataccgaat ttgttggctt tcaaacctgt ggagatgtaa
     2641 ggatgggttt tcgcatcgac atcgggattg acgcgcaggg agacgggcgc ggttttaccc
     2701 aaacgcgcgg caactttctg aatacggtcg atttcgggga tgctttccat attgaagcat
     2761 ttcacgcctg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac gcctgaaaat
     2821 atggtttttg ccgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
     2881 atgtcaaaac cgctccccaa agaagcaaag tgtttgatga tgctcaggtt gccattggct
     2941 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaatagg gtcatatttt cgttttcatt tttgggttgg
     3121 aggggcggat tgcggtttgc cttgaagctg caaaccggtt tggattacgc cgaagcgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtag ccgcaggcag agagcagcag
     3241 agccgttgct gctgcaaaaa atacgccgta tttcatcagt aaaactttct tcatcattac
     3301 gaatgtggca agattcggta tcttaaacaa aaaccacaca aaaagccatg atgaccgaaa
     3361 gcgagtttat ccgcgcgagc gaagcattat ttgaacacat cgaagaccaa atcgacgaaa
     3421 acggctggga tttcgactgc cggtttgccg gaaacgtcct gaccatcgaa gccgaaaacg
     3481 gcacgcaaat catcgtcaac cgccacacgc ccaaccaaga attatggatt gccgccaaaa
     3541 gcggtggcta ccatttcgcc gaacaaaacg gcaaatggct ggcaacgcgc gacggacggg
     3601 atttttacga cgttttaaac gaagccctga gcgcggcttc gggcgaagcg gtagagattg
     3661 aggaattgtg atttgggtgt tatcacggaa agaaaaaatg aacacacgtc ccttttattt
     3721 cggacttata tttatcgcga ttattgctat acttgctaac tatttaggaa acactgattt
     3781 ttcccatcat tatcatatca gtgctttaat tattgctatc ttgctgggaa tggcaatcgg
     3841 caataccatt tatccgcaat tttcaacaca agtggaaaaa ggtgttttgt ttgccaaagg
     3901 cgcgcttctt cgcactggca ttgtgttgta tggttttcgc ctcacttttg gcgatattgc
     3961 cgatgtagga ttaaatgcgg ttgtcactga tgcaatcatg ctaatttcaa ccttcttttt
     4021 taccgcactt ttaggcattc gttatctaaa aatggataaa caattggttt atctcactgg
     4081 ggcaggttgc agcatttgcg gtgcggcagc ggttatggcg gcagagtctg ttaccaaagc
     4141 agaatcccat aaagtttcag tggcgattgc catagtggtc attttcggga cgcttgctat
     4201 ttttacttac cccttgttct acacgtggtc acaagattta attaacgccc atcaattcgg
     4261 tatttatgtt ggttctagtg tacacgaagt ggctcaagtg tatgcgattg gggaaaatat
     4321 taatcctatc gtggcgaata ctgccgtcat ttccaaaatg atccgagtga tgatgctcgc
     4381 ccccttttta ttaatgcttt cttggttatt aacacgtagt gatggggtat cagaaaatac
     4441 ctcacacaaa attacaattc cttggtttgc tgtacttttt attggtgttg ccatttttaa
     4501 ttcttttgat ttattaccaa aagaactcgt gaaattattc gttgaaatcg attctttctt
     4561 attaatttca tcaatggctg cgcttggctt aacgacgcaa gcaagcgcaa tcaaaaaggc
     4621 aggattaaaa ccgtttgttt taggaatact aacttattta tggctagtgg ttggtggatt
     4681 tttagtgaac tatggaatat caaaattaat ataaaattca ctaaagagag cgttacccaa
     4741 tggcacaatt acccctgtat cggactgccg aaatcgaaaa ttttactgtc ggcacgcccg
     4801 aagttttaga atcattttcc aaacatatcc cttatggtgt tgtctttgaa gacgacggcg
     4861 acacaggcta cttctatgcc gcttcgcaag acgggatttt agatgccttg cacatttaca
     4921 atgtcgaaga tgtatccgat aaacatatcc ccaatcatgt cttgatttta tgggatgatg
     4981 cctgcaccat agccgcattg tgtatcaacg actacattca tgccgtctat gattttgtcg
     5041 aacaggcagg atattgccgc aacggcttcc ctgaagcaca aggcgaatgg ccgaaagtca
     5101 cagaccgcgt attggacgat gaattgcagg acaaaatcct atcccgaaaa cctacataac
     5161 ctactcaaaa ggataaccca atgcccctac tagacagttt caaagtcgat cacacccgta
     5221 tgcacgcccc cgccgtacgc gtggcgaaaa ccatgaccac gcccaaaggc gacaccatta
     5281 ccgtgtttga cctgcgtttc tgcattccca acaaagaaat cctgcccgaa aaaggcatcc
     5341 acacgctgga gcatttgttc gccggtttta tgcgcgatca cttgaacggc aacggcgtgg
     5401 aaatcatcga catttccccg atgggctgcc gcacaggttt ctacatgagc ctcatcggta
     5461 cgccttccga acagcaggtc gccgatgcgt ggctggcttc gatgcaggat gtggccaatg
     5521 tcaaagacca aagcaaaatc cccgagttga acgaatacca atgcggcact taccaaatgc
     5581 actcgctcgc cgaagcgcaa caaatcgcgc aaaacgtgtt ggcgcgcaaa gtggcggtga
     5641 acaaaaatga agagctgacg ctggatgaag ggctgctgaa cgcctaatcc gccaaaaatg
     5701 ccgtctgaac aagggtttca gacggcattt gccttttccg ttataatctg gggttgtgcg
     5761 ggggcgggtt ttaagccggc atcgtccttc cctatttttt tctgtccctt atcggtttta
     5821 agcgggtttt ttatgtccaa cagacctaca ctcctcctcg ttgacggatc gtcctacctc
     5881 taccgtgcgt atcacgcgat ggggcaaaac ctgaccgcac ccgacggtgc accgacgggt
     5941 gcgctgtatg gcgtattgaa tatgttgcgc cgtttgcggt cggaatatcc gcacgattat
     6001 tgcgcggtgg tttttgatgc gaaaggcaaa aatttccgcc atcaaatgtt tgaagaatac
     6061 aaggcgacgc gcccgccgat gcccgacgat ttgcgtccgc aggcggaagc tttgccggat
     6121 ttggtgcgcc tgacgggctg gccggtattg gtgattgggc aggtggaagc ggacgatgtg
     6181 atcggcacgc tggcaaaaca gggggcggaa catggtttgc gagtcattgt ttcgaccggc
     6241 gataaggaca tggcgcagtt ggtggatgag cgcgttacgc tggtgaacac gatgagcgga
     6301 gaaacgctgg atattgaagg cgtgaaggca aaattcggcg tgcgccccga ccaaatccgc
     6361 gattatctcg cgctgatggg cgacaaggtg gacaacgtgc cgggcgtgga aaaatgtggc
     6421 ccgaaaacgg cagtgaagtg gctggaagcc tacggctcgt tgcaaggcgt gatagaacac
     6481 gcttcggaaa tcaagggcaa agtgggcgag aatctgcgtg ccgccctgcc gcgcctgccg
     6541 ctgtcgtatg atttggtaac gattaaaacc gatgtggact tgcacgccga gctttcagac
     6601 ggcctcgaaa gcctgcgccg taccgcgccg aaatgggcgc agctggttgt cgatttcaaa
     6661 cgctggggct tccgcacctg gctgaaggaa gcggaaagcc gtatgcacga agcggcggac
     6721 ggcgatttgt tcggcagcga tacgataggc gaacaggcgg cgttggatat ggaaacgtcg
     6781 tctgaaaggc tacctgaaaa agccgttgcc cccgaaaagc tggattatca ggccgttacc
     6841 accgaagcgc agtttgccac tttgttagac aaattgtcgc aggcggaaaa aatcggcatc
     6901 gacacggaaa ccacttccct agacgcgatg aacgccgcgc tggtcggcat cagcatcgcg
     6961 ttccaagcgg gcgaagcagt ttacatcccc gtaggacaca gcctgaccgc cgcgccagaa
     7021 cagcttgatt tgcaagacgt attaggccat ctgaaaccgt atttggaaaa tcctgccctg
     7081 aaaaaaatcg ggcaaaacct caaatacgac caacacgttt tcgccaatta cggcatcgcc
     7141 ctgaacggca tcgccggcga cgccatgctc gcttcctaca tcatcgagag ccatctcgga
     7201 cacggcttgg acgaattgtc cgaacgctgg ctcggcttgg aaaccattac ctacgaatcg
     7261 ctgtgcggca aaggcgcgaa gcaaatcggt tttgccgatg tcgccatcgg gcaggcgacc
     7321 gaatacgccg cccaagacgc cgatttcgcc ctgcgcctcg aagcgcacct gcgcgcgcaa
     7381 atggacgaaa aacagcttga aatgtatgaa aaaatggagc tgcccgtcgc gcaggtattg
     7441 tttgaaatgg aacgcaacgg tgtgcaaatc gaccgcgccg aactcgcccg ccaaagcgcg
     7501 gaactcggcg ccgagctgat gaagctcgaa caagaagcct acgccgccgc agggcagccg
     7561 ttcaacctca actcgcccaa acagctgcaa gaaatcctgt tcgacaaaat gggcatcccg
     7621 acaaaagggc tgaaaaaaac cgccaagggc ggcatttcca ccaacgaggc cgtgctcgaa
     7681 cagctcgcgc ccgactaccc cttgcccaaa atcatcctgc aaaaccgcag cctggcgaaa
     7741 ctcaaatcca cctacaccga caaactgccc gacatgattt ctccccggga taaccgcgtg
     7801 cataccacct acgcccaagc cgtcgccatt acaggacgct tggcaagcaa caaccccaac
     7861 ctgcaaaaca tccccatccg caccgccgaa ggccgccgcg tgcgccgcgc ctttaccgcg
     7921 ccaccgggca gcgtcatcgt ttccgccgac tattcccaaa tcgaactgcg catcatggcg
     7981 cacctctctg gagacaaaac cctcattgcc gcattccaaa acggcgaaga cgtacaccgc
     8041 cgcaccgccg ccgaagtgtt cggcactgcg cccgaaaacg tctcgcctga gcaacgccgc
     8101 tacgccaaaa ccatcaactt cggcttaatc tacggtatgg ggcaatacgg ccttgccaaa
     8161 tcattgggca tcgacaacct ttccgccaaa aactttatcg accgctactt cgcccgctac
     8221 cccggcgtcg ccgaatacat gcagcgcacc aaagaacaag ccgccgccca aggcttcgtc
     8281 gaaaccctgt ttggccgccg cctctacctg cccgacatcc gcaacaaaaa cgccaacgcc
     8341 cgcgccggag ccgaacgcgc cgccatcaac gcccccatgc aaggcaccgc ctccgacctt
     8401 atcaaacgcg ccatgataga cgtgtcccgc tggctggttt cagacgacct gaagagcaaa
     8461 ctgattatgc aggtgcatga cgaactggtg ctggaagtcg ttgaaaccga actggatttt
     8521 gtcaaagaaa aactgccgca gattatggcg aaagtgggcg gcggattatt ggatgtaccg
     8581 ctggtggctg aggttggcgt aggggagaat tgggaagagg cacattgatt gaaaggtgtt
     8641 attatgctat ctttatttaa ataaaattta atttttggta tattttttct aaatgttcct
     8701 a
//
LOCUS       DAWFBO010000082         8576 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFBO010000082 DAWFBO010000000
VERSION     DAWFBO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.082"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(111..938)
                     /gene="fghA"
                     /locus_tag="WGO87_001327"
     CDS             complement(111..938)
                     /gene="fghA"
                     /locus_tag="WGO87_001327"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0392682.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(947..2056)
                     /locus_tag="WGO87_001328"
     CDS             complement(947..2056)
                     /locus_tag="WGO87_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0392683.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLDCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            2204..2611
                     /locus_tag="WGO87_001329"
     CDS             2204..2611
                     /locus_tag="WGO87_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0392684.1"
                     /translation="MAYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(2811..3125)
                     /locus_tag="WGO87_001330"
     CDS             complement(2811..3125)
                     /locus_tag="WGO87_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0392685.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(3136..4821)
                     /gene="rpsA"
                     /locus_tag="WGO87_001331"
     CDS             complement(3136..4821)
                     /gene="rpsA"
                     /locus_tag="WGO87_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0392686.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(4977..5633)
                     /locus_tag="WGO87_001332"
     CDS             complement(4977..5633)
                     /locus_tag="WGO87_001332"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0392687.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            complement(5877..6922)
                     /locus_tag="WGO87_001333"
                     /pseudo
     CDS             complement(5877..6922)
                     /locus_tag="WGO87_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(7059..7307)
                     /locus_tag="WGO87_001334"
     CDS             complement(7059..7307)
                     /locus_tag="WGO87_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0392688.1"
                     /translation="MKRHPTLQPLSRQHHLGLVIANKAKSVTDDDKLTQHQAFILTVA
                     GATVGFGATWRFPYLVGENGGARMCFYSVSRCWLSASR"
     gene            complement(7401..8564)
                     /locus_tag="WGO87_001335"
     CDS             complement(7401..8564)
                     /locus_tag="WGO87_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0392689.1"
                     /translation="MQRKFDLAQKPVLLSYGIDYEQEKNRQGATGYDLTTFMQSNGLA
                     FKDTGNRYTYGPDTTLKTYGIFANANVALTDDLKISAGVRHQIIDSKTDGFVPPSESL
                     LADLLNQNKVPYQAGTVNSGKVKHHKTLFNLGANYSLTPNHSIFANFGQGYSLPDLQR
                     VLRDVGTGFAVNSDNVEPISVNSYDAGWVGVFGGSRAKLTGFYNTSDKVTQFLKDYSV
                     VVADTDERIYGAEFELNHKINDNWAVGGSIAYTRGQYKDASGQYRELGAFRISPTKAT
                     AFAQYTTPSEHKLRLQLLAIDGTQRAYKDSLIAKTNQKVRKTPEARISGYVVADLLGE
                     IKLPKGQLNFGIYNLTNKKYKSVFSQAAKVTYGELSSLSAQGRSYGISYTLNY"
BASE COUNT         2152 a         2051 c         2030 g         2343 t
ORIGIN      
        1 ccgtagagtc ggctccttcc ggctcagacg gcatacgttt atgccggcac ttcttaccgc
       61 cctcgtgccg gctatcccaa aactgctttg aacactgaac gctctttggt ttacttcaaa
      121 aacgcggcgt gataagcaat atgctcgcca ataaaactgg cgatgaagta atagctgtga
      181 tcgtagcctt tatggaaacg cacatcgacc ggctggtttg ccgcacggca ggtttcgata
      241 aaatcttcgg tacgcaattg tgtcggcaaa aactcatctt ccaagccttg atcgatgcgc
      301 ataccttgca ctttatagcc ttgttgaatg agtgagttcg catcatattg ctgccatttt
      361 tcacggtctt tccctaaata agcagtaaag gctttttctc cccacggcac gaggcttggc
      421 gataaaatag gcgaaaaggc agaaacactt tgataacgtt cctgattccg cagcgccaat
      481 accaatgcgc cgtgtccgcc cattgaatgc cccataatgg aacgtttgcc gttggtagga
      541 aagtgtttct caatcagacg gggcagctcg ttcaaaatgt aatcatacat ttgataattc
      601 gccgcccaag gctgttcggt cgcattcaaa taaaagcctg caccctgtcc taaatcgtaa
      661 gcatcatcgt tcggcacttg ctctccgcga gggctggtat cggaggccac cacaattact
      721 tgatgttctg ccgcataacg ctgaaagcct gatttggtaa tgaaattttg ttccgtacac
      781 gtcaagccgg aaagccaata aatcacacca agcggtcgat tttctggatt atttggcaaa
      841 tagacggcaa atttcatttc gcattgcagc gtttgggcat gatgcgccca aacttgttgc
      901 gaaccgccaa aaatttgatg ttgttcaatc agtttcatcg cataccttag tagtgaataa
      961 cggcgcggat tgatttacct tcgtgcatta actcaaaggc ttcattgatt tgatcgagtg
     1021 tcattgtgtg ggttacaaac ggttctaact caatgtcgcc tttcattgaa tcttccacca
     1081 ttttcggcag ttcagagcga cctttcacac cgccaaatgc tgaacctttc caaacacgac
     1141 ctgttaccaa ctggaacgga cgcgttgaaa tttcttgtcc tgcacctgct acgccgatga
     1201 taatggattg tccccaacca cggtgcgcac tttctaatgc ctgacgcatt acgtttacgt
     1261 tgccgataca ttcaaaggtg tgatcaatgc cccatttatt gatgtctaac aacacatctt
     1321 taatcggttt atcgtaatcg ttcgggttca aacaatccgt tgcaccgaac tgttttgcca
     1381 actcaaattt tgatggattg gtatcaatgg cgataatgcg gccggctttg gcttgacgcg
     1441 caccttgcac caccgccaaa ccaatcgccc ccaaaccaaa cacggcaaca gagtcgcctt
     1501 cttgcacttt tgccgtatta tgtaccgcac caatacctgt ggtaacgccg cagtcgagca
     1561 aacatacttg ttcatggttg gcttcagggt tgattttcgc cagtgaaact tcggcaacaa
     1621 cggagtattc actgaaagtc gaacagccca tatagtgata aatcggctga ccttgataag
     1681 aaaaacgcgt cgtgccgtcc ggcattaagc ctttaccttg tgtatcacgc actgagacgc
     1741 acaagttggt tttacctgaa caacaaaact cacattcgcc acattcggcg gtgtaaagcg
     1801 gaatcacgtg atcacccggt tttacgcttg acacaccttc gcccacagca acgaccacac
     1861 ccgcaccttc gtgtccaagc accacaggga atacgccttc aggatcgctt cctgataacg
     1921 taaacgcatc agtatggcac acgccagtgt gggtattgcg gattaacacc tcgcctttac
     1981 gcggcatttc tacgtcgatt tccacaattt gtaagggttg gttaggggcg aatgccaccg
     2041 ccgcacgaga tttgatggtt gaatcggttt gtttcatttc cattgtcttt cctcttaatt
     2101 cattgacaaa gttgccttac ctgagcaaca tagcaacagt ttacacttta gagttaactc
     2161 taaagcaagt gttttttact gtttttgttt agaaggaggg aaaatggctt ataccactgc
     2221 caaagccgcc gaaaaaatag gcatctccgc ccacacccta cgtttttacg acaaagaagg
     2281 cttgttgcct aatatcggac gtgatgaata cggtaaccgc tgttttaccg ataacgattt
     2341 gcaatggttg ggcttattgc aatgcttgaa aaatacgggg atgagcttaa aagacatcaa
     2401 acgctttgcg gaatgtaccg tcattggcga cgataccatt gaagaacgcc tttccttgtt
     2461 tgaaaatcaa atagaaaatg tgaagtgtca aattgccgaa ttaaaacgct atttagattt
     2521 gcttgaatac aaattggcgt tttaccaaaa agcgaaagca ttaggctcgg taaaagctgt
     2581 aaatttgccg caaattcctg aaacggctta gttttagtgc aataagcagg atttttgtcc
     2641 tgttcgtaat agaaatcaaa aatttgttgt ttaaaaagcg gattgtaagc aatgataaaa
     2701 aaatccacgc tctaatccgt tgcttgcttg tcttaaggta ttgaagcagc ggatgaagtt
     2761 aaaccacaga aaaaaaacgc cgcttggaag cggcgttttg atgctaaggt ttaattggca
     2821 ttttctttta aagccaaatc gacccgctcg cgcaattctt taccgggctt gaagtggggt
     2881 acatgttttt caggtacttc cacacgctcg ccggttttgg gattgcgacc gatgcgggca
     2941 ggacgatggt tcaaatcgaa gctgccgaaa ccgcggattt cgatgcgttg acctcgggca
     3001 agcgatctag tcatggtgtc aaccaagact tttacgctgt actctacgtc ttttgccaga
     3061 agatgcgtgc cgtttttggc ggcaaacact tctgccaaac gaaccattaa ctcagacttt
     3121 gtcatgtctg caaccttatt cttgttcgcc ggagagtttg gctttcagca ggtcgcccaa
     3181 gctggtggtg ccggcattcg cattggcggc ggcattgacg gagttcagtg cttcgcggct
     3241 ttctttggca tctttggctt taacggaaag tttgatgctg cggtttttgc ggtcaacggt
     3301 aacgatgacg gcttcaactt cgtcgccttc tttcagtttg gtggtcaaat cttcaacgcg
     3361 gtcggctgca aattcggaag caggcaggta gccttctact tcgtcagaca gggcgataac
     3421 agcacctttg gcgtcaacag atttcacgga acctttaacc aaagaacctt tgtcgttcac
     3481 gctgatgaag ttgccgaacg gatcgccttc cagttgtttg atacccaagg agatgcgttc
     3541 tttttccacg tcgattgcca atacgacggc ttcgacttct tcgccttttt tgtatttgcg
     3601 tacggcttct tcgccggatt cggtccagga caggtcggac aggtgaacca aaccgtcgat
     3661 gccgccaggc agaccgacga atacgccgaa atcggtaatg gatttaaccg cgccggagat
     3721 tttgtcgcct ttgttgtggt tggcggcaaa ttcttcccaa ggattggctt ggcattgttt
     3781 catacccaaa gagatacgcc ggcggctttc atcgatttcg aggatcatta cttcgacttc
     3841 gtcgcccaat tgtacaactt tgctcgggtg tacatttttg ttggtccaat ccatttcgga
     3901 aacgtgcacc aagccttcga tgccttgttc gatttcaacg aacgcaccgt agtcggtcag
     3961 gttggatact ttaccgaaca ggcgggtacc ttgcgggtaa cgacgggtca ggccgctcca
     4021 aggatcttcg cccagttgtt tcatacccag agaaacgcgt tgtttgtctt ggtcgaattt
     4081 caggacttta gcttctactt cttgaccgac ttccagaact tcgctcgggt gtttcacacg
     4141 gcgccatgcc aagtcggtga tgtgcagcag accatcgatg ccgcccaagt caacgaatgc
     4201 accgtaatcg gtaatgtttt taacgatgcc tttgatgacg gagccttctt gcaggttttc
     4261 cagcagggct ttgcgttctt cacccaaggt agcttccaga acggcgcggc gggacacaac
     4321 aacgttgttg cgttttttgt ccagtttgat cactttgaat tcaatttctt taccttcgaa
     4381 gtgagaagtg tcttttacag gacgtacgtc gaccaaagaa cccggcagga atgcgcggat
     4441 gctgctaatc ataacggtca ggccgccttt gactttgccg ttgatgatgc cggacaggat
     4501 gttgccgttt tccatggctt cttccagggc aatccaatcg gcagcgcgtt tggctttttc
     4561 gcgggacagt ttggtttcgc cgaagccgtt ttcgacggat tcgatggtaa cggtaacgaa
     4621 gtcgccgact ttaacttcaa tttcgccttg agcgtttttg aattcagcta catcaatcag
     4681 ggattctgat ttcagacctg cgtttacggt aacgaagttt tggtcgattg ccactacttc
     4741 agcggtaatc acctcacccg ggttcatttc ttgcagggta aagctttctt ccaacagctg
     4801 agcaaaattt tccatagaca tatataactc ttttcggtac accgccaagg ggtgcggggt
     4861 gggttggtgg atatctgccg tccttggcag ggcagacggg tacataaggt ttcagacggc
     4921 atatggggga tttttatgcc gtctgaaacg taatttgtgc gattatacct gaaaatttaa
     4981 acttcacgat accaatcaag cacttttttt acagtttctt ctatagtcag gcggcttgtg
     5041 tccaaaagca gggcatcggg ctgttgtttc aggggggcaa ctttgcggtt tcggtctgcc
     5101 tcgtctctgg cttcgatgtc ggacaggatg cgctcgaatg ccaaaccttc gcaggggatg
     5161 ccgatttgtt tggcgcggcg ttcggcacgg attttggatt ctgccgtcag gaagatttta
     5221 agttcggctt gggggaagac gaccgatccg gtgtcccgtc cgtcggcaac cagtcctttt
     5281 tcggtcagaa aatcgcgttg gcgttgcagc agggcggcgc ggactttagg caactgtgcg
     5341 actgcggatg cgcccatgcc gatggcttct gtccggatgc cgtctgaaac gtcttcgccg
     5401 ccgagcagga tgcggctgcc tgaaaatacg gcgggcagtt tttttgccag ttcggaaacg
     5461 ttttcttcat cgtgccatcc cacgccttgt ttttgtgcat atagggcagt caggcggtag
     5521 agtgcgccgg tatcgagata atcgtatccc aatgcggcgg caacgcgggc ggcaaccgtg
     5581 cctttgcccg atgcgcccgg gccgtcgatg gcgatgactt tttgtctgtt cataaggggg
     5641 attcctgatg gtttggggta tgggttttgc cgtctgaagg atgtgtttcc cgttggggcg
     5701 gattctacct gttttaaagg acgattgtct aagcagacgg aacgccgcct gcccgaaaac
     5761 atccgacaga agccggcaag ccggttcgga ttccggcgtt gcgggtattg tggcgggcat
     5821 caaggcaatg ccgtctgaaa gaggtatggc cttcagacgg catcgattgc tgcagattag
     5881 aacaggttgc tgacaaaaat cagcaaaatc accaacggca caagatattt cacataagca
     5941 aaccaaatat tgaccgtcgt atggttgcct ttgtaaagca attcttcctt cgcttcgtcc
     6001 ttcatcacaa aaccgacaaa cagcgcgaaa ccgagcgcgg tcagcataaa caagatgttg
     6061 ccgctgatgt agtcgaaggc atcgaaaata tttttgccga acacggaacg tctttccacg
     6121 aaccatagct cagaatggac gggatgttgc cgaaaatgaa gatggcggct aatacaatcg
     6181 taatcgcggc ggtacggcgg attttggttt tttcctgaat ggtcgtaatc aacacttcat
     6241 aaatggtcag cgaagtcgtc aatgcggcaa tcaggagcag cgagaagaaa atcacggcga
     6301 acacagatcc tgcccacata tgcgagaaca caatcggcaa actttggaat accaaagtcg
     6361 ggccggaatc gggggcaacg ccgaagctga agagcgacgg gaaaatcata aagccggcca
     6421 gtacggcgat gatggtattg gtaatggccg tgatgaccgc ggtttgcacc agattttcgt
     6481 tcttatccag atagctggac aaggtaatca tcacgccgaa acccaagctc aaggtaaaga
     6541 acacttgtcc taaaacgaag acgaacagtt cggctgtaat tttgctgaag tcaggtttca
     6601 gatagaaggt aatgccttcc attgcgcccg gcagggtaac gttgcgcaca accatggcaa
     6661 tcaggaataa aaacagcaaa ggcatcaggt attttgctgc tttttcaata ccgccgatca
     6721 cgccttttac caagatccat tggttcacaa ccacaaatag gaaggtgtag aaagcaattt
     6781 cccaaggact gttttcgatg tgttcggtaa agaaactttt ggtgacttcg ctggaaacag
     6841 gactggaaat gtccaaatta ccgccgatga tattaacaat gtagctgata acccagccgc
     6901 cgagcaccat gtaataagcc atgatgccga acgcgccgag cagcccatcc agccgaccag
     6961 tttccaaatt ttggcaatgg gtttgccgtt catcgggccg ccgaacgcat ccagcgcgtt
     7021 cacgcctttg cgccgtccga tgacattttc caccaaaatc atcgggatgc cgataaccag
     7081 catcgcgata cagaataaaa acacatacgc gcaccaccgt tttcaccgac caaatacggg
     7141 aaacgccacg tcgcgccgaa accgacagtc gcgccggcaa cggtcaggat aaaggcttga
     7201 tgttgggtaa gtttgtcatc atcggtgacg gatttggctt tgttggcgat gaccaagccc
     7261 aaatggtgct ggcgagatag gggctgtaag gtggggtgtc gtttcatggg ggttcctgtt
     7321 ggtaaattaa attttattat aaaataaaaa aaccacaaac actagggcga tgtttgtggt
     7381 attgtggcac ataatgctga tcaataattt aaggtataac ttatcccata acttctcccc
     7441 tgtgcagaca agctggataa ttcaccataa gtcactttgg cagcttgact aaatacactt
     7501 ttatattttt tatttgtcaa gttataaatc ccaaagttta actgaccttt aggtagtttg
     7561 atctcaccca ataaatccgc cacgacatag cctgaaattc tagcctccgg ggttttgcgg
     7621 accttttgat tggttttggc aatcaggctg tccttgtaag ctctttgtgt accatcaatg
     7681 gcaagtagtt gtaagcgtaa cttatgttca cttggtgtgg tgtattgagc aaaagcggtg
     7741 gctttggtag ggctgatacg aaacgcccca agttctcgat attgaccgct tgcatctttg
     7801 tattgacctc gtgtataggc aatgctacca ccgacagccc agttatcatt aatcttgtga
     7861 tttaattcaa actccgcccc ataaatacgc tcatcagtat cagcaaccac cacgctataa
     7921 tcttttaaaa actgggttac tttatctgat gtattataaa aacctgtcaa cttggcacgg
     7981 ctaccgccaa acactcctac ccaccctgca tcataactat tgactgaaat tggctcaaca
     8041 ttatcagaat taaccgcaaa acccgtccca acatctctta gcaccctttg taaatcaggc
     8101 aaactatatc cttgcccaaa attggcaaaa atactgtgat ttggagttaa tgagtaattt
     8161 gctccaagat taaataaggt tttgtgatgc ttcactttgc cagaattgac agttcctgct
     8221 tgatacggca ctttattctg gtttaataaa tcggctaata gcgactcact gggtggcaca
     8281 aaaccatctg ttttactgtc tataatttga tgccgtacac ctgccgatat ttttaaatca
     8341 tctgtcaaag caacattagc attggcaaat atgccgtagg tttttaaggt ggtgtcaggt
     8401 ccataagtgt aacggttgcc agtgtcttta aatgccaagc cgttgctttg cataaaggta
     8461 gtcaaatcat agcctgtcgc cccttggcgg tttttttctt gttcataatc gataccataa
     8521 ctaagtagta caggtttttg agcaaggtca aattttcttt gcaaagccag tcttgc
//
LOCUS       DAWFBO010000083         8457 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFBO010000083 DAWFBO010000000
VERSION     DAWFBO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.083"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            79..1263
                     /gene="coaBC"
                     /locus_tag="WGO87_001336"
     CDS             79..1263
                     /gene="coaBC"
                     /locus_tag="WGO87_001336"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0392690.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPIIVANDVSIAMGKPTNQITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1330..3486)
                     /locus_tag="WGO87_001337"
     CDS             complement(1330..3486)
                     /locus_tag="WGO87_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0392691.1"
                     /translation="MPAPQPSAPYDPLTVEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTREMDAVAEGGIAGHWSYKSDSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPIDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDSE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3578..3784)
                     /locus_tag="WGO87_001338"
     CDS             complement(3578..3784)
                     /locus_tag="WGO87_001338"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0392692.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3844..4461)
                     /gene="gmk"
                     /locus_tag="WGO87_001339"
     CDS             complement(3844..4461)
                     /gene="gmk"
                     /locus_tag="WGO87_001339"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0392693.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSQEVIQRRLLKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            4589..5188
                     /locus_tag="WGO87_001340"
     CDS             4589..5188
                     /locus_tag="WGO87_001340"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0392694.1"
                     /translation="MALKTSNMEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5234..6022)
                     /locus_tag="WGO87_001341"
     CDS             complement(5234..6022)
                     /locus_tag="WGO87_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0392695.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAATGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDSL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVKREGLKTVRWHEEAVDLLPAGASKMDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6347..7702
                     /gene="yegQ"
                     /locus_tag="WGO87_001342"
     CDS             6347..7702
                     /gene="yegQ"
                     /locus_tag="WGO87_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0392696.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHDRNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPGGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7836..8312
                     /locus_tag="WGO87_001343"
     CDS             7836..8312
                     /locus_tag="WGO87_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0392697.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPIANGRNLVTRQAANSVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT         2145 a         2313 c         2000 g         1999 t
ORIGIN      
        1 tccaaacggt aactaatgta tccgcacttt gttatagaat ggctgctgtt tttttcttcg
       61 taattagaaa ttgtcaaaat gggtaaacat attcttttag gtgtaacggg cagtattgcg
      121 gcgtataagt cttgcgagtt ggttcgtctg ctgaaaaaac aggggcattc ggttacggtg
      181 gttatgagcc gctcggcaac tgaatttgtt tctccgctga cttttcaggc tttaagcggc
      241 aatcctgtcc tgaccgacac gcacggcggc aacggttcaa acggtatgga acatatcaac
      301 ctgacccgga atgcggatgt ttttctgatt gcgccggcaa gtatgaatac cgtggcaaaa
      361 atctgtaacg gcgtggcaga taacctactg accagtctgg cagccgcacg gaaatgtcca
      421 ctcgccatcg ctcccgcgat gaatgtggaa atgtggctca accctgccaa ccaacggaat
      481 atcgcacaac tggtttcaga cggcattact gtctatatgc cgggcttagg cgaacaggct
      541 tgcggagaaa atggtatggg aaggatgccg gaacctgccg aattgctgga tttgcttccg
      601 gacttgtgga caccgaaaat tttaaggggc aagaaaatct tgattaccgc aggtgcgaca
      661 tttgaagcca ttgaccctgt caggggcatc acaaatacct ccagcggaaa aatgggggta
      721 gctttggcgc gggcgtgccg tgccgccggt gcggaagtca gcctgattca cggacagctt
      781 caaaccgcgc tgcctttcgg catatccgat acggttcaag ccgtcagcgc tgaagatatg
      841 catcgcgcag tgcatcgttt gattgaaaag caagatgctt ttatttctgt tgccgccgtc
      901 tcagactata aggttaaaaa caagagtacg caaaaattca aaaaagataa aaatgccaaa
      961 ccgttatcca tcgaattgga tgagaacccc gatattttgg cttctattgc ctcattgcca
     1021 aacccgccgt tctgcatcgg ttttgccgct gaaacggaga atgtaatggc atatgcacga
     1081 gaaaaacgta ttaagaaaaa aataccgata atagttgcca atgatgtttc aatcgcgatg
     1141 ggcaaaccga ccaaccagat taccattatc ggggacgacg gggaactatc ttttcccgaa
     1201 acaagtaaag atgaagcggc gatgcagatt gttgaaaggc ttgccgtata tttgaacaaa
     1261 taagcaattg aacggataaa ccataaagcg ggttgcctgt taatcaaaag gcaacccgct
     1321 ttacctgctt caacttctga tgactttgcg gatatatgga atactatgca gattttgaat
     1381 aatctgattc aattgattca gattcttgac tttcaataag aatttgaatt cgacaaaacc
     1441 ttccgttccc gactgggatt tagacggtgt ttcgaccgac tcaatgtccg caccggaatc
     1501 ggaaatcgct tgcgccatta atgccaacag gccgtggctg tcttccgatt ggacttgaag
     1561 cccgacacgg tagttctgcc cattcatatt ttcccagtct gcatccagct gctgttcgga
     1621 atcggacttc aacaacgtcg ggcaggtatc cctatggata atcatgcctt ttcccttaac
     1681 caacagcaaa cggatggaat caccaggaac aggatggcag cactctgcaa aatgaatatg
     1741 cccgctttcc tgaccatcga ctttaatgga actgagcctg acctcgctgc cgaaatgctc
     1801 ccctgccaac tcggcaatgt gcatggcgac ataaacaggc agggtatgcc ccataccgac
     1861 gttgtacagc acttcctcaa atgaagtctg tttgtcgttg agatcggcaa ggtatttttc
     1921 tttgatgccg tctgaaagca ggacatcttt gggcagcaaa ctcgacaggg ctttttgtaa
     1981 gaggctctct cccaaaacga ccgcatcgtg ccggttaagg tttttaatat attggcgtat
     2041 ggcactgcgc gccctgccgg acacggcgaa attcaaccat gcaggatttg gtttggcatg
     2101 ttcggatgta ataatttcga cggaatcgcc ggttttgagc ttcgtacgca acggcatcat
     2161 gatattgttg atacgtgcgg caacggtttt gtgcccaata tcagtatgca ccgcataagc
     2221 aaaatcgata ggtgttgcac ccttgggcaa tgttaggatt tttccttttg gcgtaaggat
     2281 atagatttcg tttggaaaca aatctacctt gacgtgttca agaaactcaa tagcattggc
     2341 actgctggcc tgcaaatcta agatattttt cagccaccgg tttgtgtgaa gcaccgcctg
     2401 atcgaccgtc ttagaatctg atttatagct ccaatgtccg gcgataccgc cttcggcaac
     2461 agcatccatt tcccgcgtgc gtatctgaac ttcaatcggc aagccgtaag ggccgaccaa
     2521 agtcgtatgc agactttgat aaccgttgct tttcggaata gcgatgtagt ctttgaaccg
     2581 cccgggcttg ggctgataaa gggtgtgcaa tgcgccgagt gcggcataac aggcgggaat
     2641 gctgttgaca atgacgcgga aaccgtaaat atccataacc tcggcaaagc gcagcttttt
     2701 cgccatcatt ttctgatgga tgccgtacag gtttttttcc ctgcctttga ttttggcctc
     2761 tatattcgcg cctaccagcc tctgaccgaa tgcgcgcaag actttgccga caacgtcctg
     2821 ccggttcttc cggctcttgt ccattgcttt ttttaaagtc tcgtaacggt tgggatgaag
     2881 gttttggaac gataaatcct gaagctcccg atacgcatta ttcaaaccta tacggttggc
     2941 aatttgcgcg tagatttcaa gggtttccct tgcaatccgg cggcgtttgt ccgggcgcat
     3001 cgaaccgagt gtccgcatat tatgcagacg gtcggcaagt ttaacgacaa tcacgcgtac
     3061 atctttggtc attgccaaaa tcagtttgcg gaaactctcc gcctgatgct ctgcatgatc
     3121 ttcaaatttg agtttttcaa gcttggacag cccgtccacc atctcggcaa ccgtattgcc
     3181 gaacaccgcc gccatttccc cttttgtcac gcccgtatct tccaatacgt cgtgcatcac
     3241 gcctgcacaa agaccctgta tgtccatatg ccaaagggcg agctgcgtcg caacggcaat
     3301 cggatgcgtg atgtagggct ccccgctttt ccgggtttgc ccgtcgtggg cacgaaacgc
     3361 ataggcgaca gctttttcaa gctccgcctg ttcctcgggc ttgaggtagg aggcggtatg
     3421 gaaaagcagg gcacgcgctt cgacggtcag ggggtcgtaa ggggcggaag gttggggggc
     3481 gggcatttca gacggctttc ggtatgtatg tgtttttcat ttcaaaccgt cggactgcac
     3541 ggcggcaaag tgttccggcg tgcgtttccg gcagaattta tttattgcgc gtcaacagtt
     3601 ctgtaccgat atgtccggcg gcgatttccc ttaaggcggt aacggtcggt ttgttattgc
     3661 ggacatcgtc cacaagcggc gtattgccat tttcaagctg gcgggcccga cgggccgcta
     3721 ccaatgtcag gtcaaaatgg ttggaaattt ttccggtaca gtcttcggtg gtaatacgtg
     3781 ccatattatt tgctttcttt caaaaatatt taaattggga aaccaggtat tttcgcggtt
     3841 tttttaggaa ttttccaaca aatctgcaat aaatcccagt tgccgcgacc ttttcagacg
     3901 gcaggcattc acaatatgga gcaaatcctc ctccgcccgc gccaagtcgt cattgaccac
     3961 gacaaagtcg aacaatacgg actgttcgat ttcatgcctt gccttcaaca gcctcctttg
     4021 gataacttcc tgactgtccg tcccgcgtcc gttgaggcgc gcggcaagta cgtcgaaaga
     4081 aggcggcagg ataaagatgc cgacggcttc cggcagcgcg tcgcgaacct gcgccgcgcc
     4141 ctgaacgtcg atttccaaaa tcacatcata gcctgccgcc gccaacgcat tcacgccctc
     4201 cgcgcttgtg ccgtaatagt tgccgaatac gtctgcgtat tccaaaaaag cttcctgcgc
     4261 gataagcgac tcaaactctt ctttggaaac aaagtgataa tgtacgccgt ttgcttcgcc
     4321 ttcacgcggc gggcgcgtcg tgtgcgacac ggaaacgcgc aaaccgttat ggtttgccaa
     4381 tagccgagaa accagcgtgg ttttgcccgt gccggaagcg gccgaaatga taaagatgtt
     4441 gccttttcga taagcggaca tattttttac ctgtatattt tccagccgat tgtatcacaa
     4501 cggacactcg gtttcctatt tgccgatgcc catattttgc cgctattgtt ttgattgatt
     4561 tggcaagcgg caggctgacg gctacaatat ggcgttaaaa acatcaaaca tggaacacgc
     4621 aatgctggtt catcccgaag ctatgagtgt cggcgcgctt gccgacaaaa tccgcaaaat
     4681 cgaaaactgg ccgcaaaaag gcatcttatt ccacgacatc acgcccgtcc tgcaaagcgc
     4741 ggaatacttc cgccttttgg ttgatttatt ggtttaccgc tatatggatc agaaaatcga
     4801 catcgttgct ggtttggacg cgcgcggctt cattatcggc gcggcactcg cctaccatct
     4861 caacgtcggt tttgtcccca tccgcaaaaa aggcaagctg ccttttgaaa ccgtatcgca
     4921 aagctacgcg ctcgaatacg gggaagctgc ggtggaaatt cacaccgatg ccgtcaaacc
     4981 cggttcgcgc gtgctgctgg tcgatgattt ggttgccacg ggcggcacga tgcttgccgg
     5041 actggagcta atccgcaaac tcggcgggga aattgtcgaa gccgccgcca ttttggaatt
     5101 taccgacctt caaggcggca agaatatccg tgcaagcggc gcgcccttat ttaccctact
     5161 tcaaaacgaa ggctgcatga agggctgaaa accgaccctg ccgtctgaaa ccggcagggt
     5221 tgttattatg cgttcaaatc acacccaaat cttgcaagcc cctcaacacg ccgtcttcat
     5281 caacgccggg gcaaacatat ttcgccgctt ctttcgccgc ctgttccccg ttgcccattg
     5341 ccacgccgaa cccgacttct gacagcattt ccacatcgtt caaaccgtcg ccgaacgcca
     5401 tcacgtccgc catttccaaa cccaatgctt caaccacgct tctgataccg tccattttcg
     5461 acgcacccgc aggcagcaga tcgaccgctt cctcgtgcca gcgcaccgtt tttaagcctt
     5521 cccgtttcac aatatccgac cagagcggca tttcgttttc ctccgcgaac accagcatct
     5581 gatacaccgg tttgcttgaa aaataatcct tatcggcaaa aaaatcgctg gcgatatgct
     5641 tcaaggcgcg gcacacgcat tccgacagcg cggacacggc gatcccctct ccgccgacaa
     5701 acgcataatc catacccaag ctatccaaat gcgcgcaaac cctgcccatc aaaccggcat
     5761 ccatcggtac ttcgcgcaca gttttaccgt gcagcagcgc aaactgtccg tttatcgtta
     5821 ccacggcatc catacccgtt gccgccatca tgtccctgac cttttcgggt atcgtcgcca
     5881 aagaccgccc cgttgccaac gccgtcaata tgcctttgcc gcgcaaagcc gccaccgccg
     5941 ttttcacgga aggacgcaag gtatccgtat atttgcggta cagcgtatcg tcaatatcga
     6001 aaaacacgat tttgggattc atcatattct ctctcgcact caaactgccg cattgtatcc
     6061 caatcaggca aatacttgat aaatccttat aaatttcccg gcaaaattga ccgcgaacac
     6121 gaaaaggcgg ataatccgcc catcttcaaa cacccttcag acggcatttg cagcaatgcc
     6181 gtctgaaaca tttttacaaa gcacacaaat catgtttcaa cacacaggac gacacataaa
     6241 gcgtcgccct atatgttgcc ctgattcgga aagggttacg tccctcccaa ataaagtctg
     6301 attctaccgc cctaaagggc ggggtttcaa ccgaaaagga aacacgatga aagcacccga
     6361 acttttatta ccggcaggcg gtctcgaacg tatgcgcgcc gcctacgatt acggcgcaga
     6421 tgccgtttac gccggcagcc cgcgttactc cctgcgcgcc cgcaataacg aatttgccaa
     6481 actcgacgtt ctcgagcaag gcattaaaga agcgcatgat cgcaacaaga aattcttcct
     6541 taccgtcaat acgctgccgc acaactccaa actcaaaacc ttcgtttccg atatggagcc
     6601 cttgattgcc atgaaacccg acgcgctgat tatggcggat ccgggcttga ttatgaccgt
     6661 gcgcgaaaaa tggccggaaa tgccgatcca cctgtccgta caagccaaca ccaccaacta
     6721 ctggggcgtg aaattctggc agaatatcgg cgtcgaacgc atcatcctgt cgcgcgaatt
     6781 gagtatggaa gaaatcgccg aaatccgcca acaatgcccc gacatcgaac tcgaagtctt
     6841 catccacggc gcattgtgca tcgcctactc aggccgctgc ctgttgtcgg gctatttcaa
     6901 ccaccgcgac cccaatcaag gcacctgcac caactcctgc cgttgggact acaaagtcca
     6961 caacgctacg gaaagtgatg caggcgatgc ccagcttctg caaggtttta actttgaaaa
     7021 agcccaagaa gaagccaacc aaaactttga aggcatcaac ggtcaaaaac gccatcccta
     7081 cgccgacaaa gtcttcctga ttgaagagtc caaccgcccg ggcgaaatga tgccgattat
     7141 ggaagacgaa cacggcacct acatcatgaa ttccaaagac cttcgcggca ttgaagtggt
     7201 tgaaaaactc gccaaaatcg gcgtggacag tctcaaagtc gaaggccgca ccaagtcgct
     7261 ttattatgtt gcacgtgtcg cccagtccta ccgcaaagcg attgatgatg ccgttgcagg
     7321 ccgtccgttt gattacagcc tgttgagcga actcgaaggc ctcgccaacc gcggctacac
     7381 cagcggcttc ctcgaacgcc accaaaccca agactatcaa aactacctga ccggccactc
     7441 tattgccaaa caaagccaat acgtcggaca cgttaccgaa atcgacgaaa acggctgggc
     7501 gaccatcgaa gtcaaaaacc gcttcgccgt cggcgattca ctcgaaatca tccacccggg
     7561 cggcaaccaa accatcaaat tggaacaaat ggcccgcaaa ggccagcctg tcgatgtcgc
     7621 acccggcaac ggcattcagg tcaaaatccc cgatatgcag ggcaaagaaa aagccctcat
     7681 cgcacgcgta ttgaacccct gagccacaat gccgtccgaa ccccgttttc agacggcatt
     7741 ttgttgaatc ttacggtaca atggcaatga tttcccacag caaacaagga catccgacat
     7801 gaaaaaaaat cctcatactc tccgccgccg ccctgatgct ggcagcctgc caatccggca
     7861 gcgcacctaa aaacgcgcac acggcatccg ccgacacacc ggccggagaa tcatgcaaag
     7921 tcatccggga cgacaccgca ggaagcggca aaaacatcat ttaccgttgc tgcaacggtc
     7981 aggcggcaat aaatgcagcc attgcagacg gcgtattgga atcagggatt cccgtcagct
     8041 tcggcggcgg gggcgtaccg attgcaaacg gcaggaatct ggttacacgg caagcagcaa
     8101 acagcgttgg caaatccgac tccgaggcct gcgaacacgc cctaatcaat gccgcccgca
     8161 aattccagca gaccgccggc aaactcggcg ggcgcagtgt taccggcttc cacagctact
     8221 tcgacaagca accgctgcaa ggaggacaat acgactgtca ggcaggctcg ttccacgtcc
     8281 gcgtggtcat gcgcgccaat gtcgtccgtt aacatatcgg cgaccaaaaa attaggctgt
     8341 gttttagtaa tctgttgatt tcaattattt gcaaaggaaa agacaattat tttttcggtt
     8401 aggaagagac ctatcctatt gaatacattt aagttaaata cgtctatcaa cactata
//
LOCUS       DAWFBO010000084         8245 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFBO010000084 DAWFBO010000000
VERSION     DAWFBO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.084"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WGO87_001344"
     CDS             complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WGO87_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0392698.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFSKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1658..2041)
                     /locus_tag="WGO87_001345"
     CDS             complement(1658..2041)
                     /locus_tag="WGO87_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0392699.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2045..3586)
                     /locus_tag="WGO87_001346"
     CDS             complement(2045..3586)
                     /locus_tag="WGO87_001346"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0392700.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGWAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQPPSKKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            3876..4709
                     /gene="serB"
                     /locus_tag="WGO87_001347"
     CDS             3876..4709
                     /gene="serB"
                     /locus_tag="WGO87_001347"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0392701.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLSDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4773..5996
                     /locus_tag="WGO87_001348"
     CDS             4773..5996
                     /locus_tag="WGO87_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0392702.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVARASEGRRLGVLILCGMLAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6157..7737
                     /gene="purH"
                     /locus_tag="WGO87_001349"
     CDS             6157..7737
                     /gene="purH"
                     /locus_tag="WGO87_001349"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0392703.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIHPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1830 a         2261 c         2334 g         1820 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 tcactgcaaa ccttcataaa cggggtttgc agtgattttt tcaaatcaaa cagattgaaa
      181 acctgcgccg aattgttcag acggcattat tttttcagtt cggacagaat gtcatccacg
      241 gttttcttcg catctccgaa acacattacg ctgttttcgt tgaagaacag cgggttttgt
      301 acacctgcgt agccggtatt catcgagcgt ttgaagacga cgacttcttt tgccttccat
      361 acttccaaca cgggcatacc cgcaatcggg ctgttcgggt cggtttgggc ggcaggattg
      421 acggtatcgt tcgcaccgat gaccaagacc acatcggttt cggggaaatc gtcgttgatt
      481 tcgtccattt ccaacacgat gtcgtagggg actttggctt cggcaagcag tacgttcata
      541 tgaccgggca ggcggccggc gacggggtgg atgccgaagc gcacttctgt gccgttttta
      601 cgcaaaagct cggtgatttc ggcaacgggg tattgcgctt gtgcgactgc cataccgtag
      661 cccggggtaa tgatgacact gtttgcgcct ttcagcattt cggcaacatc ggcagctttg
      721 acttctcggt attcccctat ctcttggctg ccggaagatg atgtgccgct gtcgctgccg
      781 aaaccgccgg cgattaccga gacaaacgac cggttcatgg ctttgcacat aatgtaggac
      841 agaatcgcgc cgcttgagcc gaccaacgcg ccggtaacga tgagcaggtc gttggagagc
      901 ataaagcctg ccgctgcggc cgcccagccg gagtaggagt tgagcatgga cacgaccacg
      961 ggcatatctg cgccgccgat ggaggcaacc aagtgccagc cgaatgcgag ggcaatcagg
     1021 gtcataatca gcaggatgaa gccgctgccg tcaatgccga cgaatacgag cagcaacaca
     1081 aacgatacgc caagtgccag tgcgttgagc ttgtgtttgg cgggcagttg cagcgggctg
     1141 ctgctgattt tgccgttgag tttgctgaat gcgaccagcg agccggtaaa ggttaccgcg
     1201 ccgatgaaga tgcctaaata cacttcgacc agatggatgg tgtgcatatc gtgcgaaacg
     1261 ttgcccggcg cgatatagct gttgaagccg accaaaaccg ccgctaggcc gacgaagctg
     1321 tgcagcaggg caatcagttc gggcatttcg gtcatttcca cctttttggc tttgtagatg
     1381 ccgattgccg cgccgatgag catggcgatg atgatccagc ccagtccgtg ggtattgtcg
     1441 gaaaaaacag ttacaaaaag ggcgaccgcc ataccggcga taccggaata gcagccctgt
     1501 ttggcggttt cctgtttgga cagccccgcc agtgagaaga tgaataaaat tgcggcaacg
     1561 atatacgccg ctgttacgag tcctgaagac atggaaattc tccgattttc gatgatttgt
     1621 tttcaatgcc gtccgaactg ttcagacggt atctgtttca agcagccgcg acaaacagcc
     1681 cgacggtgca ggcaaatccg cctgcccaca tcagtgagcg catagcggct ttgtcggcga
     1741 tatagcacca gataaaggcg aggcggaaca ggatgaacag gcaggcaagc gtgttgatgg
     1801 tcgattgcgc cgcattgccg gttgcgtgtg ccgtcaaaac ggcggcggca aacggtgcaa
     1861 aggcttcaaa accgttttgc tgcgcggcgt gggcacgggc ggctgtgcct tgcgtgtgcg
     1921 ccagaaaatc gcgcggattg tggttgtctt taaaccggaa tccgcccgct tttttggcat
     1981 atgccgcaca aaaaagcggc aataggcagg caatcaggat gcaccaatag gcgaaagtca
     2041 tggcttaccc tttcttaaac atattcagca tacgccgtgt aaccgcaaag ccgccgaaga
     2101 tgttgatgcc ggcaatcagg atggcaacaa acgacagcag cgaaacaaag ccgttgccct
     2161 gaccgatttg cagcagcgcg ccgacgacga tgatgccgga gatggcgttg gtcacggaca
     2221 tcagcggcgt atgcagcgag tggctgacat tccaaacgac gtagtacccg atgacgcagg
     2281 cgaggacgaa cacgataaag tggttcaaga atgctgcggg tgcgaccgcg ccgacccaca
     2341 gcactaatac ggcggcgatg acggcaggcg cgagtttttt ccacagggga acgggtttcg
     2401 gctcgggctt ggcggcaggc gcggcttttt tagacggcgg ttgctgcggc tgggcggaaa
     2461 cttgaatcgg cggaggcggg aaggtgattt cgccgtcgcg ggtaacggtc atgttgcgga
     2521 taatcacgtc ttcaaaatcc agcgtgattt cgccgtcttt gttcgggctt aacagcttgg
     2581 tcaggttcac caagttggtg gcgtaaagct gggaagactg tccggcaagg cggtttgcca
     2641 tgtcggtgta gccgatgatt ttcacgccgt tgccggttac ggacaattcg cccgggcggg
     2701 tgagttcgca gttgccgccc gtcgccgccg ccaaatcgac gatgacggag ccggatttca
     2761 tgctttccac catttctttg gtaatcagct tgggcgcggg tttgcccgga atggcggcgg
     2821 tggtgatgat gatgtccact tctttcgcct gctcggcaaa gagcttcatc tcggctgcga
     2881 taaattcgtc gctcatcact ttggcgtagc cgtctccgct gccgcccgat tcttgtggga
     2941 agtcgagttt caggaacttg ccgcccatcg attcgatttg ttccgccact tccaagcggg
     3001 tatcgaacgc gcgcaccact gcgccgagcg agtttgccgt accgatcgcc gccaaacctg
     3061 ccacacctgc gccaatcacc aaaacctgcg cgggcggcac tttgccggcg gcggtgattt
     3121 ggccggtgaa gaaacggccg aaggcgttgg cggcttcaat tacggcgcgg tagccgctga
     3181 tgtttgccat cgaagacaaa gcgtccaaag cctgcgcgcg cgaaatgcgg ggcaccatgt
     3241 ccatcgccag cgcgttcact ttcttggcgc gcaaggcttc gaccaaagcc tcgttttggc
     3301 gcggccacag gaagctgacg atggtttgac cttcgttcaa aagcggcagc tcgccttcgg
     3361 acggcgcatt gaccttataa atcaaagggc aggcccaaac cgccgctttg tcggcaacgg
     3421 tcgcgcctgc tgtttggtaa gcggcatcgt ccaaacttgc cgcccaacct gcaccgcttt
     3481 cgacaacggt ttcaaagccc agtttgccca gcagggcgac ggtggcgggc gtacaggcga
     3541 cgcgggtttc gccggataat gactcgcgtg ggataccgat tttcatctct gaatcctttt
     3601 tcgggttgtt tatatgtatc gtgggttaaa tttaaatcgg ggcggggcgg agcaacgccg
     3661 taccggttta aaaccgattc acttcaaatg ttaatatatt ttagataatc cccttataac
     3721 gaattttcat caggctggca atagttgcgg cattttcccg tgttgtccga cacatactgc
     3781 cgtttgcgtt tgaagtcatt ttccgattgc agtcccgccc gccgtaacat tctttgccgt
     3841 ttcactatat aatccgcatt ttttgagccg ccgttatgcc gcacgccctc gtcctccaat
     3901 ttccctccgc cgaagccctg ccttccgact tcccctcccg cctgcccgaa cctgattgcg
     3961 ctgatgaaaa gcgtatgcgt tttattgttg aagaaggatt ttctttaagc gaaaaagacg
     4021 cggcgttact tggcagccgt caaatcgacc acgccgtgtt gtcggatatg gattttggcg
     4081 aactcggttt gattgtcagc gatatggatt cgacgctgat taccatcgaa tgcgtcgatg
     4141 aaattgcggc aggcgtgggt ttaaaagaca aggtagcgga aattaccgag cgttcgatgc
     4201 gcggagagtt ggatttcgag cagtcgttgc gcagccgcgt tgcgctgttg gcgggattgg
     4261 acgaacagat tttggcggac gtttatgaaa acgttttgaa gctctcgccc ggtgcggaat
     4321 ttttgttgga cgaatgcaaa aggcacgatg tgaaattcct gctggtgtcc ggcggcttca
     4381 cgttttttac cgaacgtctg caacaacgcc tcggcttcga ataccaacac gccaatgttt
     4441 tggaaattga aaacggcagg ttgaccggcc gtctgaaagg cagaatcatc gacgcgcagg
     4501 caaaggcgga tttattgcgc gaataccgca gccgcctcgg attgcagcca catcaggtgt
     4561 tggcgatggg cgacggtgcg aacgatattc cgatgctcaa agaagcgggc ataggcgtgg
     4621 cttaccgtgc caaaccgaaa gcgcgggccg ccgccgatgc ctgtatcaat ttcggcggtt
     4681 tggagcgtgt acgcggcctg ttcggatagg cggataggaa acggatgtcg tccgaaaggt
     4741 tttcagacgg catttgaacg gcaggaacga cagtgggacg cagaaaactt tggtttgccc
     4801 tggcagcagc aggcgttatc ggcggtttgg tcggcattgt gctgacggaa ctgatgcact
     4861 tcatacagca tacggcatac ggttatggcg cggacggtgc gtacatctcg ttccgcgaag
     4921 gtgtggcccg tgcttcggaa gggcggcgtt taggcgtatt gattctgtgc ggtatgttgg
     4981 caggcggcgg ctggtggttg ctcaaacgtt tcggtaagcc gcaaatcgaa atcaaagccg
     5041 ccttgaaaca gccgttgcag gggctgccgt ttctgacgac ggttttccat gttctgctgc
     5101 aaatcataac ggtcggactc ggttcaccgc tcggacggga agtcgccccg cgtgaaatga
     5161 ctgccgcgtt cgcttttgcc ggcggcaggc ggttgggttt ggatgaagac gaaatgcggc
     5221 tgctgattgc ttgcgcttcg ggtgcgggtt tggcggccgt gtataacgtg ccgctcgcct
     5281 ccacactttt tatcctcgaa gccatgctgg gcgtgtggac gcagcaagcc gtcgccgccg
     5341 cattgctgac ttccgtcatc gccaccgccg tcgcgcgcat cggcttaggc gacgtgcagc
     5401 aatatcatcc cgccaacctt gccgtaaaca cttcattact ttggttttcc gccgtcatcg
     5461 gcccgatact gggcgcaacc gccgtctttt tccagcgcac cgcccaaaaa ttccccttca
     5521 tcaagcgcga caacatcaaa atcattccct tggcagtctg catgtttgcg ctcatcggcg
     5581 tgattgccgt ttggtttccc gaaattttgg gcaacggcaa agcaggcaac caactgacct
     5641 ttggcggttt gaccggttgg caacacagcc ttgagctgac cgccgtcaaa tggctggttg
     5701 tcttgatggc gttggcggca ggcgcatacg gcggactgat tacgccgtct atgatgctcg
     5761 gcagcaccat ttcttttgca gccgccgccg cgtggaacag cgttttcccc gaaatgccgt
     5821 ctggaagcgc ggcagtcgtc ggagcggcgg tgtttctcgg cgtatcgctc aaaatgccgc
     5881 tgaccgccat tgtgttcgta ttggaactga cctacgcgcc gctgtccctg ctgctgcctt
     5941 tgtgcatcgg catggcgggg gcattgggaa catcgggaaa aatgggcttc aaataaatgc
     6001 cgacaaatgc cgtctgaaag gttttcagac ggcatttctt tgtaaattgt cagcaatatc
     6061 tgcaaaaacc tactgccaaa aaactagaaa tagcggataa taccgccccg aaaaagttcc
     6121 cctttcccca tccaactgtc agaaaaggaa gccccaatgt ccgtcatcaa acgcgccctg
     6181 atcagcctgt ccgacaaggc aggcgcggtc gaatttgccc aaactctgca caagctcggt
     6241 gtcgaaatcc tctccaccgg cggcacggcg aaactgcttg ccgatgcggg cgttcccgtg
     6301 attgaagtcg ccgactatac cggcttcccc gaaatgctcg acggccgcgt gaaaacgctg
     6361 catccgaaaa tccacggcgg tattttgggt cggcgcgatt tggacgaaca cgttgccaag
     6421 atggaagaac acggtatcgg caacatcgac cttgtgtgtg tcaacctcta ccccttcgcc
     6481 gccaccatcg ccaaaccaaa ctgcacactg gaagacgcga ttgaaaacat cgacatcggc
     6541 ggcccgacta tggtgcgttc cgccgcgaaa aactggaaac acgtcgccat cgttaccgac
     6601 accgccgact tccccgccat tgccgccgaa ctcgaagcca acaacggcgc attgagcgac
     6661 aaaacccgtt tcaacctctc acgcaaagca ttcagccaca ccgcccaata cgacggcatg
     6721 atttccaact acctgacctc cctttcagac gacgttttga gcggccagcc ccaaatcggc
     6781 gaattcccaa gccagttcaa ccaaagctgg attaaagtac aagacatgcg ctacggcgaa
     6841 aatccgcacc agcgcgccgc gttctaccgc gatattcacc ccgccgccgg cagcctcgcc
     6901 gcctacaaac agctgcaagg taaagaattg tcttacaaca acatcgccga tgccgatgcc
     6961 gcttgggaag ccgtcaaatc cttcgacgcg cccgcctgcg tgattgtgaa acacgccaat
     7021 ccgtgcggcg ttgccgttgc agccgatacc ttgaccgcct acaaactcgc ctacgccacc
     7081 gacaccacca gcgcgttcgg cggcatcatc gccttcaacc gcgaagtgga cggcgaaacc
     7141 gtcaaacaga ttaccgacaa ccagtttatg gaagtcttga tggcgccgaa gtttaccgcc
     7201 gaagccctcg aaatcgccgc cgccaagaaa aacgtgcgcg tattggaagt gccgcttgag
     7261 gcaggcgcaa accgcttcga actcaaacgc gtcggcggcg gactgttggt acaaacgccc
     7321 gacatctgcc gcatcagccg cgccgatttg aaagtcgtct ccaaacgcca accgaccgag
     7381 caggaatgga acgatttgct gttcgtctgg aacgtcgcaa aatacgtcaa atccaacgcc
     7441 atcgtgttcg gcaaaggcgg acaaacctac ggcatcggtg caggccaaat gagccgcgtg
     7501 gacagcaccc gcatcgccgc ccgcaaagcg caggacgcca atttcgacct caacggcgcg
     7561 tgtgccgcct ccgatgcctt cttccccttc cgcgacggcg tggatgtgat tgccgaacag
     7621 ggcattaaag ccatcatcca tccggcaggt tcgatgcgcg atcaagaagt cttcgacgcg
     7681 gcagacgaac acggcattgc gatggtggta accggcatcc gccacttccg ccattaaggc
     7741 agatgaacaa ggcaatgccg tctgaagggc tttcagacgg cattttgcgc tattttgtga
     7801 aggtagggat gacggttcgg gtattcctga cagggtggat tttcaaggtg ttgtataggg
     7861 tgtaggagga ttcgtaaaag gtgggatgca gggtgtgctt cagcccgctg catcaaaaat
     7921 ttttggagaa ccggcgggag tcggcggttt tggtttcggc ggggacggtg gaaatgggta
     7981 acattgacgg aatcgacgga agcggtgggc tgaagcccac ccttgtatat tggaatcact
     8041 gtatcatagc aacaaaccgc ctggccgcca cccgcgccca cccaaggcac acaaccgttg
     8101 cgtagctcag ggagcggcag ggcaacccat cgacacaacc ggacagttgc cggacaacac
     8161 aaccgaatgc aaggcaggtt tatgatgagt acccaatacc attacgcagg tatagtgaat
     8221 taaatctaaa ccagtacagc gttgg
//
LOCUS       DAWFBO010000085         8196 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFBO010000085 DAWFBO010000000
VERSION     DAWFBO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.085"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(95..1027)
                     /gene="cysK"
                     /locus_tag="WGO87_001350"
     CDS             complement(95..1027)
                     /gene="cysK"
                     /locus_tag="WGO87_001350"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0392704.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVPNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1221..1826)
                     /locus_tag="WGO87_001351"
     CDS             complement(1221..1826)
                     /locus_tag="WGO87_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392705.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1823..2674)
                     /gene="dapF"
                     /locus_tag="WGO87_001352"
     CDS             complement(1823..2674)
                     /gene="dapF"
                     /locus_tag="WGO87_001352"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0392706.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAEWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVEQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2811..3107
                     /locus_tag="WGO87_001353"
     CDS             2811..3107
                     /locus_tag="WGO87_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392707.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPTVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3121..3789
                     /locus_tag="WGO87_001354"
     CDS             3121..3789
                     /locus_tag="WGO87_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0392708.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3862..5982)
                     /gene="pnp"
                     /locus_tag="WGO87_001355"
     CDS             complement(3862..5982)
                     /gene="pnp"
                     /locus_tag="WGO87_001355"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0392709.1"
                     /translation="MFNKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6219..7082)
                     /locus_tag="WGO87_001356"
     CDS             complement(6219..7082)
                     /locus_tag="WGO87_001356"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0392710.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAEEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7281..8144
                     /gene="rfbD"
                     /locus_tag="WGO87_001357"
     CDS             7281..8144
                     /gene="rfbD"
                     /locus_tag="WGO87_001357"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0392711.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1954 a         2368 c         1902 g         1972 t
ORIGIN      
        1 gttttcggca tatatgcaaa aacgcatttt ccggcacggc gttacaccga taccgaaaaa
       61 tgcgtctgaa tgtttcgaca atccgattaa agcattatgc caaatctgca aaaagtggcg
      121 tagagagata gcgttcgcca taagaaggca gcagcacgac tatcagcttg ccttcgtttt
      181 caggctgttt ggcaagctgc aacgcactcc aaaccgccgc accggaagaa atacccacca
      241 aaatgccttc tttttccgct attgcgcggg cggtttcaaa agccgcttcg ttcggcactt
      301 tggtaatgct gtcgtaaatt ttggtattca aaacggtcgg aataaagcct gcgccgatgc
      361 cttgaatcgg gtgcgggcct ttttcgccgc cgctcaatac gggtgaagct tcaggctcga
      421 cggcaaccac tttaacttcg ggtttgtatt ttttcaacac ttcgcccacg ccggtaatcg
      481 taccgcccgt gccgacgccg gcaacgaaga catcgacttt gccgtccgta tcccgccaaa
      541 tttcctcggc ggttgttttg cggtggactt cggggtttgc ctcattgtcg aactggcgcg
      601 gcataaaata agtgtcggga tgcgcgtcca ccaaggattt cgctttggca atcgcgcccg
      661 ccataccttc ggcggcaggg gtcagaatca gctccgcacc aaacgcgcgc aacagcattt
      721 tgcgctcctt actcatgctt tccggcatgg taatcgccag cttgtagccg cgtgcggcac
      781 ataccattgc caaaccgaca cccgtattgc cgctggttgc ttcgacaatg acggtgtttt
      841 tgttgatttt gcccgctttt tcggcaccct cgatcattgc ttcggcaatg cggtctttga
      901 cgctgctgcc tggattgaaa aattccagtt tcacggcaac ctccgccttc aaaccttcgg
      961 tcagacggtt cagtttgacc aaaggcgtgt tgccgattag ttcggtgatg ctgtttgcaa
     1021 ttttcatctt ctgctcctga ataataaggt ttcgacagta aggcgcaatc cccataggca
     1081 tcctgtccga ttgcagggga tttgcgccga aacataaaca aatttcggat atggctttat
     1141 cctaaaccta tgccgcctaa aaaaccaata tccaaaattt ctttctttat aactttttgt
     1201 tccgatacgg ccgcaccgta ttatcttcca atttctgaaa gcagcagcac cagtaccgag
     1261 acctgcatca acgctgccgt caacccgccg agtcgcagct gcgagccgcc gaacgttgac
     1321 ggcggctgcc ggtctatacc gcagcggtgg acataggcaa ccgccatcag cacaaactgc
     1381 aacacgaacc aataaaccgg cttcagcgca taaagtttga gcagtgccgc aaacacaaac
     1441 acttgaaccg ttcccgaaaa gcaataatgc accaacgcac atattccgac aaaagcaagc
     1501 gtcatcgata cattactgac ggcaaaaagc ccgagcagcg gatgttcggg gtctgcctgc
     1561 catccgtttc catctgtttt ccggttccaa tgcccgatga aaaaaaatat gctgcccaaa
     1621 gtcaggtaaa aatgggggat gaacaagggc gcggcgcggg tcattcccca tatgccgggc
     1681 atcagctttg cccccaaaac cgatatgccc agccacagca taatactcgc ccaaaaccat
     1741 tgcaacattc ctgcgcggaa tatcagaaac agaaaaaccg ccgcatacag taaggcagac
     1801 aaaaaatcca aatgcagcaa aatcatgaat acgccaactc gccttcaaac accgtttccg
     1861 cagggccggt catcatcaca tcgccgccgc aggcccattc gatatataaa gttccgcccg
     1921 gcaaaatcac ctctaccgtt ttcccttcat ccaacaaccc cagacggata cccgccacca
     1981 cagccgcaca cgcgcccgta ccgcacgctt gggtttcgcc cacgccgcgc tcgaacacgc
     2041 gcaaacgaat cgcggttcgg ctgacaatct gcataaagcc gacattgacg cgttcgggaa
     2101 actgcctgtg cggttcgata agcgaaccgg tttcgcgcac cggcgcgcat tccacgtcat
     2161 cgaccacaat caccgcatgg ggattgccca tattgacgca gctgacaggc tgaatgccgg
     2221 attcgagatg caccccgtaa atacaggcat catccccctc gcccgattcg gggacaaacg
     2281 gtatttcaga cggcataaac ctcggtttgc ccatattgac cgtaaccatg ccgttatcgg
     2341 acaatttcgg aaaaataacg ccatttgccg tttcaacaca aatttctttc ttatcggtca
     2401 aacccttgtc tgcaacaaaa cgggcaaaac aacgcgctcc gttgccgcat tgctcgacct
     2461 cgctaccgtc ggcattgaaa atacggtaac ggaaatccac accttcggtt tccgaacgcc
     2521 cgaccaccaa aagctggtcg aagcccacgc cccggaagcg gtccgcccat tccgcaatcg
     2581 gcgcgtcctc gggggtaaaa tcctgactga ccgcgtcaat caccataaaa tcgttgccca
     2641 aaccgtgcat tttggtaaat ttcaaagtgt tcatattgtt ccgaaatccc gtatcgtctg
     2701 ttgcttccga atccgaaatc ccccgcaccg aagggaaatg cttccatctc ccgccgcctg
     2761 aggattaaaa tcagccatta tactgtttgt taccccttac aagaaacacc atgttccgca
     2821 tattactgtt gcttttccct tgtgcaatcc ttgccgcctg cacccgaaac ataccggcaa
     2881 acccgaccgt ttcaaattgc gcgcatcttc aaaaccgcac catcctgtat tttgacgcac
     2941 acggcaaacc cgccgacggc gcagtaaagg gcggatttta caaaattatc gaaatacagg
     3001 gtgcaaactg catccttatt caagacttta acagcacggg caatgccctc ggcggatcct
     3061 atgtgccgga ctgccgcaaa tcatcccttt tcccgaaaaa tatttgactt caaactgcct
     3121 atgaaccacg acatcacttt cctcaccctg ttcctactcg gtttcttcgg cggaacgcac
     3181 tgcatcggta tgtgcggcgg attaagcagc gcgtttgcgc tccaactccc cccgcatatc
     3241 aaccgctttt ggctgatcct gctgcttaac acaggacggg taagcagcta tacggcaatc
     3301 ggcctgatac tcggattaat cggacaggtc ggcgtttcac tcgaccaaac ccgcgtcctg
     3361 cagaatattt tatacacggc cgccaacctc ctgctgctct ttttaggctt atacttgagc
     3421 ggtatttctt ccttggcggc aaaaatcgag aaaatcggca aaccgatatg gcggaacctg
     3481 aacccgatac tcaaccggct gttacccata aaatccatac ccgcctgcct tgcggtcgga
     3541 atattatggg gctggctgcc gtgcggactg gtttacagcg cgtcgcttta cgcgctggga
     3601 agcggtagtg cggcaacggg cgggttatat atgcttgcct ttgcactggg tacgctgccc
     3661 aatcttttgg caatcggcat tttttccctg caactgaaaa aaatcatgca aaaccgatat
     3721 atccgcctgt gtacgggatt atccgtatca ttatgggcat tatggaaact tgccgtcctg
     3781 tggctgtaag ccccattcat tcccgatacc cgtaaaaaat accgtctgaa acctgttcag
     3841 acggcacttt caccctaagc gttactcggc ggcattttcc tcacgggcag gcgcgtccag
     3901 cagggctttg atggacagac ggacacggcc tctgtcgtcc acttccaatg ctttcacgtt
     3961 caccacctga ccgacttgca ggtagtcgcc gacattgcgt acgcgctcgt gggcgatttg
     4021 gctgatgtgt accaaaccgt ctttgcccgg catcacactg acaatcgcgc cgacattgtt
     4081 gtcgaggatt ttcaccacag tgccttcgta cactttgccc acttccactt cggcagtaat
     4141 ctgctcgatg cgttttttcg ccgcatcgcc ggcttcttga gtggttgcgg caatggtaat
     4201 cgtaccgtct tcggcaatat tgatttccgt accggtttca gcggtaatcg cacggatggt
     4261 ttcaccgccc ttaccgataa cttcgcggat tttgtcttgg ttgattttca tcgtgaacaa
     4321 gcgtggcgcg tgtgcggaca gctcttgcgg gcccgcaacg gcggctttca tctgatccaa
     4381 gatgtgcaga cgcgcttctt tggcctgcgc caaagcgatt tgcataatct ctttggtaat
     4441 gccttggatt ttgatgtcca tttgcagcgc ggtaacgcct tcggtcgtac cagccacttt
     4501 aaagtccata tcgcccaagt ggtcttcgtc gcccaaaatg tcggtcagga cggcaaactt
     4561 attgccttcc agaatcagac ccatcgcgat accggcaacg tgtgctttca aaggcacgcc
     4621 ggcggacagc aggctcaagc agccgccgca gacggaagcc atagaggaag agccgttgga
     4681 ttcggtaatt tcggagacca cgcgcatggt gtagctgaaa tcttcaggtt tcggcaatac
     4741 ggccaacaat gcacgtttag ccaaacgacc gtgaccgatt tcacggcgtt tcggcgcgcc
     4801 catgcggccc acttcgccgg tagagtacgg cggaaagttg tagtgcagca taaagcggtc
     4861 ggtgtattcg ccggacagcg cgtcgatgat ttgctcgtcg cgcgaagtac ccaaagtcgc
     4921 tacggcaagg gcttgggttt cgccacgggt aaacaatgcg gaaccgtgcg tgcgcggcaa
     4981 tacgccggtt tggatgttca gcggacggac ggtgcgggtg tcgcggccgt cgatgcgcgg
     5041 ttggccgtcc aaaatttggc tgcggacgac atcggcttcc aagtgtttga aaatgccttt
     5101 gatttcgttg gctgccagcg tgtcggtttc ttcggtaatc aaggcttctt ttaccgcact
     5161 ccaagcttcg tccaatttgg cagaacgcgc ttgtttttga cggattttga acgcttcttt
     5221 aatggtttcg ccggcaatcc cgcggacttt ggcaaccagt tcctcattgg tttcaggtgc
     5281 tttccaatcc caaagttccg gattgacttc gtcggcaaat tcattgattg cattgatggc
     5341 aacctgcatt tgatcgtggc cgtaaaccac cgcgcccagc atcacgtctt cgggcaggat
     5401 tttggcttcg gattccacca tcaacacggc ttttgaagta ccggcgacca ccaagtccaa
     5461 ttgcgatttc gccaattcgg ctttagtcgg gttcaaaacg tacacgccgt ttatataacc
     5521 cacgcgtgcc gcaccaatcg gaccggcaaa cggtacgccg ctcaacacca gcgcggcaga
     5581 cgcgcccaac attgcaggaa tgtcggaatc gatttcggga tcgacggaca cgaccatcgc
     5641 tacgatttgg atgtcgtggt agaaaccttc agggaacagc ggacgaatcg gacggtcgat
     5701 cagacggctg gtcaggattt ctttttcgct ttgtttgcct tcgcgtttga agaaaccgcc
     5761 gggaattttg cctgcggcgt aagtgcgctc gaggtaatcg acagtcagag ggaagaagtc
     5821 ttggccttct ttcacttctt tgttggtggt aacggcaacc aaaaccacgg tgtcgcccat
     5881 agagacttta acggcagcgg cggcttggcg ggcgatttcg ccggtttcca aagtaacggt
     5941 ttgattaccg tattggaagg tcttaacgtg tttattgaac attattgttc ctttcaaaat
     6001 accgcactgc taaaacacta ataatgcaca ctaaaatccg aatgtgcata gttagggttt
     6061 cagaccgtgc ggcaggttgt aaacaagctt tcagacgaca tttcggacgc tgaaagcaat
     6121 tccagattat aaaggcaacc atccttaaaa tccagtattg atacaataaa aaggccgtct
     6181 gaaccatatt tctttcagac ggccttcaaa cttaaaaatc aatcctgagt cagcaaagtc
     6241 aatgcttccc gatacttctc gacagttttc tgaatcacat cggcaggcac tttcggcgca
     6301 ggggcttttt tgttccaacc gctttgttcc agccagtcgc ggacgaattg tttgtcaaaa
     6361 gacggcggat tggtgccgac tttgtattgg tcggcaggcc aaaaacggct cgaatcggga
     6421 gtcaatacct catccatcag cgtcagcgta ccgtttgtat ccaaaccgaa ttcaaatttg
     6481 gtatcgcaaa tgatgatgcc gcgcgatttg gcatattccg ccgcttctgt ataaagctga
     6541 atcgctttgg cgcgcacttc ttccgccaat tctttgccga taatgcgttc gcattcttca
     6601 aagctgatgt tttcatcgtg atcgccgact gcggctttgg ttgagggcgt aaaaatcact
     6661 tcaggcagtt gttgcgcttc ctgcatacct tcaggcagtt gaataccgca aaccgagccg
     6721 gttttttgat aatctttcca accgctgcct gccagataac cacgcacaat cgcctctact
     6781 ttcaccggag tgagcttttt agccacgacg gcgcgtttct ctaaagcttt ggcttcgttt
     6841 tcaggcaaaa catcgtaaac cgtttgaccg gtaaagtggt tgggcataat atgcgccagt
     6901 tttttaaacc aaaaattgga aatctgcgtc agaatctccc ctttgctcgg aatcgggtcg
     6961 tccaaaatca catcaaacgc ggacaggcgg tcggaagcga ccatcagcat acgtttatcg
     7021 tcgatttcat ataaatcgcg cacttttcca gaatagatct ttaccaaacc aatctcactc
     7081 atttcgcccc ccctgaaaat atcttgaaaa taccgacccg acacccgaca ggtttgaatc
     7141 acaaaccgat attctaaccg aagtcggcgc aaaacaatac ccatggcaca aaaagccaac
     7201 ccgtcaaccg tcggcaaagt tttggcacta taataccgac agcaagtcct acaatacact
     7261 ttcaccaaag gaaatacctc atgagaatcc tattgacagg ctcgaaaagc caactggcac
     7321 gctgcctgcg cgaccgtctt ccggaagact gggaaaccat tgcgacggat tccgcatccc
     7381 tagacattac cgatgccgat gccgtctgca acatggtcaa aagtttccaa cccgacgcca
     7441 ttgtcaacac ggctgcctat actgccgtcg acaaagcgga aggcgatgcg gcagcggcat
     7501 ttgccgtcaa tgcttccgcc gtttacaacc ttgccttggc agcacatcgc gcccatgccc
     7561 gattcatcca catctcaacc gactatatct ttgacggtaa agggaaaagg ccctatcagg
     7621 aaagcgactt taccaatcct tccaatgtat acggacaatc caaaaccgcc ggcgaactgc
     7681 tcgcactgtc tgccaatccc gacagcctta tcctgcggac ttcttggctg tttagcgaat
     7741 acggggacaa ctttgtccgc accatgctga accttgcgcg ggaacgttcc ccgctgtccg
     7801 ccgtccacaa ccaaatcggc tgcccgacct atgccggcga cttgtccgcc gccatcatcc
     7861 gcctgttgca gcagtccaat cccgttcgcg gcatttacca ctacgtcggc ggcaaatccg
     7921 tatcctggta cgaatttgcc caacatattt tccaagcggc atcgcaacag cagacatcct
     7981 tccccgttcc cgaattgact gccgtttcag acaaggaata tccgaccgcc gcccccaggc
     8041 ccgcatacag cattttggac tgccgcaaaa tcgaaaacga cttcagcatc aaaccgtcag
     8101 actggcaaaa agcccttgca caggtcgttt ccaagctgct ctgatgccgc ccgcctctcc
     8161 gtttccgccg tcaagcaccg ccttggcggt tttctt
//
LOCUS       DAWFBO010000086         8147 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFBO010000086 DAWFBO010000000
VERSION     DAWFBO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.086"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            235..1167
                     /gene="pip"
                     /locus_tag="WGO87_001358"
     CDS             235..1167
                     /gene="pip"
                     /locus_tag="WGO87_001358"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0392712.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIQHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
     gene            complement(1224..2420)
                     /gene="bamC"
                     /locus_tag="WGO87_001359"
     CDS             complement(1224..2420)
                     /gene="bamC"
                     /locus_tag="WGO87_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0392713.1"
                     /translation="MPSEAFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKISQDSIRRLFDTVGLGGIYSTGERDKFIIRIEQGQNGVSDIFFAHK
                     AMKEVYGGKNKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPILPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAITE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2429..3304)
                     /locus_tag="WGO87_001360"
     CDS             complement(2429..3304)
                     /locus_tag="WGO87_001360"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0392714.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMTNDTILRLSEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDQLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            3342..3596
                     /locus_tag="WGO87_001361"
     CDS             3342..3596
                     /locus_tag="WGO87_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392715.1"
                     /translation="MLPIVAYFTAGVKSGGSGMHGAAWRRMRRVCIFVFEIRLLRFAN
                     DIPAATVRNLFAGRARMPSEIEIEAVWRIRYNPRFGFFNI"
     gene            3611..5002
                     /locus_tag="WGO87_001362"
     CDS             3611..5002
                     /locus_tag="WGO87_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0392716.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(5064..5816)
                     /gene="rlmB"
                     /locus_tag="WGO87_001363"
     CDS             complement(5064..5816)
                     /gene="rlmB"
                     /locus_tag="WGO87_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0392717.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(5882..6370)
                     /locus_tag="WGO87_001364"
     CDS             complement(5882..6370)
                     /locus_tag="WGO87_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HEZ0392718.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWLVGLISLAGILTDYVAGIWGTKYTGAGKLAVRGALIGSIIGMFFSLPGL
                     VLGPLIGAAAGELISCRNMIQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(6375..7094)
                     /locus_tag="WGO87_001365"
     CDS             complement(6375..7094)
                     /locus_tag="WGO87_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ0392719.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQTGAVQTFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMAQAHKARLLAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIKRVIYGAVEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRSVLSCFFQNKRKG"
     gene            complement(7098..8039)
                     /gene="miaA"
                     /locus_tag="WGO87_001366"
     CDS             complement(7098..8039)
                     /gene="miaA"
                     /locus_tag="WGO87_001366"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0392720.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSHAERSFVPHHLIDIITPVQTYSAARFVEDCTHLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDGQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFHMMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTHLIEAAKRFFGV"
BASE COUNT         1491 a         1898 c         2661 g         2097 t
ORIGIN      
        1 gtacggcgtt gcctcgtctt agctcaaaga gaacgattct ctaaagtgct caagcaccaa
       61 gtgaatcggt tccgtactat ctgtactgtc tgaggcttcg tcgccttgtc ctgatttttg
      121 ttaattcact atatcgtgat ttttcggcat ttgcctttcg gggcggcttg tgtttcgtgc
      181 gtgatgttgc gtgtgggaat gttcggattg tcagaggcaa tatgggagaa gatgatgtat
      241 gagataaaac agccttttca tagcggatac ttgcaggtgt ctgaaattca tcaaatttat
      301 tgggaagagt cgggcaatcc cgacggtgtg ccggtcattt ttttacacgg cggaccgggg
      361 gcgggggctt cgcctgaatg tcggggtttt ttcaatcccg atgtgttccg catcgtcatt
      421 attgaccagc gcggttgcgg acgttcgcgc ccgtatgctt gtgcggaaga caatacgact
      481 tgggatttgg tggcggatat tgaaaaagtc cgtgaaatgc tgggtatcgg gaaatggctg
      541 gtgttcggcg gttcgtgggg cagtactttg tcgctggctt atgcccaaac ccatccggaa
      601 cgggtaaagg gattggtgtt gcgcgggata tttttgtgca ggccgtctga aacggcgtgg
      661 ctgaacgagg cgggcggtgt gagccggatt tatccggaac aatggcaaaa atttgtcgcg
      721 ccgattgctg aaaatcggcg gaaccggctg attgaggcgt atcacgggtt gctgtttcat
      781 caagatgaag aagtgtgcct gtctgccgca aaggcttggg cggattggga aagctatctg
      841 atccgtttcg agccggagga agtggatgaa gatgcttatg cctcgctggc aatcgcgcgt
      901 ttggaaaacc attattttgt caacggcggc tggttgcagg gtgatagggc gattttgaac
      961 aatatcggca aaatacagca tatcccgacc attatcgtgc aggggcggta tgatttgtgt
     1021 acgccgatgc agagtgcgtg ggcgctgtcg aaagcctttc cggaagcgga attgagggtg
     1081 gttcaggcag ggcatcgtgc gttcgatccg cctttggcgg atgcgttggt tcaggcggtt
     1141 gaggatattt ttcctcggtt gttgtaaaaa gttccgcaca aaaaagcagc ttctgtttgg
     1201 aagctgcttt tgttttgaac ggtttaacgc agttcggaat ggagtttgcc caataatgcg
     1261 gatgcgtctt tgccggcata tgcgctgccg tctttgttga gcaggacgat gcgcgagccg
     1321 ttggcgacag gctcggcata gacaatcagt tccggctgtt cggcaggttt ctccgctttg
     1381 cctttgccca gcaggcgttt gaacaggccg ggtttttgtt cggtaattgc attgctttcg
     1441 ttcggggctt tttgaaccag gaaggcgtgg cgttcggtgt tttgaccgac gacggtcagc
     1501 ccgatgcggt cgagggcgag cgcggtgcgc cgccagtttc tgccgtagtc gccaaagaca
     1561 atcaggcttt tgccttcgat acgcgccatt tcgttggcgg cgggaaggat cggttttttt
     1621 gccgatgcgt tttccgcctg ctgtccgtca acgcccaaat attgcataaa gcgcgtcagg
     1681 aaagcggctt cgaggttggg atcggacggg gagggctgcc atacggtcgt gtctttgttt
     1741 ttgccgccgt acacttcttt catggctttg tgggcgaaga agatgtcgga aacgccgttt
     1801 tgaccctgtt cgatgcggat gatgaacttg tcgcgttcgc cggtggagta gatgccgccc
     1861 aagccgactg tgtcgaagag gcggcggatg ctgtcttgcg agattttggc gcggttttcc
     1921 gcccactcgg tttccatttg tccgatggcg ggttcttcgg atttgatgtc gaagccgttt
     1981 tcctgccaaa aggctttcag gagcggccag atttcggcat gagacttgcc gtcgacaacg
     2041 agccagcgtt ggctgccgtc gcgctcgagg cggacacctt tgacgctttt caatacttcg
     2101 gcatcggcag gctgttggac ggcgggtgtg cggcgttttt ccaaatcgct ggcgcggacg
     2161 gcgcccgaac cggcaggcag gcggtagagg ttgccttggt cggggttgtt caaatcaggt
     2221 gggacttcga gtttgatcag gcggtgcgac cggctttggt agtcgagctt gggctgttcg
     2281 gttttgctgc cggagcaggc ggcaagcccg atgagtgcga gcgcggcaat gacgggtttg
     2341 atatgggtca tcgtgtcatc ctgtgtgatg gatattaaag tgtttgttgc gttatgccgt
     2401 ctgaacgctt cagacggcat ggctatattt aaagttgtcc tgaggctttc agggcggcgc
     2461 ggacttttgc ctgtccgcct tccgtcagcg gaacgagcgg caggcggacg tgcggttcgc
     2521 atctgcccag ggcggatacc gcccatttcg gtgcggcggg gctgggttcg cagaacatgg
     2581 tgtcgtaaat cggaatcagc tggtcgttga gttcgcgtgc aagggcgata tcgccttgaa
     2641 gcgcggcgcg gcacatatcg gcaaagagct tgggcgcggc gttggcggct acggtaatca
     2701 cgccgtgtcc gccgcagagc atgaacggta gggcggtgtg gtcgtcgccg gacaggacga
     2761 cgaagccttc gggggcgcgg ttgatgagtt cgatgttgct gccgacgttg ccgctggctt
     2821 ctttaacgcc cacgatattg gggatttctg aaaggcgcag gatggtgtcg ttagtcatgc
     2881 tgacgacggt acggccggga acgttgtaga gtatcatcgg aatcgcggcg gcttcggcaa
     2941 cggctttgaa atggcggtac atgccttctt gcgaaggttt gttgtaatag gggacgacgg
     3001 agagggtgta gtccgcgccg gctttttcgg cggcttggga aagggcgatg gcttcgacgg
     3061 tgttgttcgc ccctgtgccg gcgatgacgg ggacgcgttt ggcaacgtgt ttgacgacgg
     3121 cttcgataac ggcggtgtgt tcttcgacgg agagggtggc ggattcgcct gtcgtgccga
     3181 cggcgacgat gccgtccgtg ccgttttcaa tgtgccagtc gattaagtcg cggagttgtt
     3241 cgtaatggat gctgccgtct tgattcatcg gggtaatcag ggcaaccagg ctaccttgta
     3301 acatacagaa ccttttatca gttgtggtgt agggggcggt aatgcttccg attgtagcct
     3361 actttaccgc aggtgtgaaa tccggcgggt ccggtatgca cggggcggct tggcggcgga
     3421 tgcgtcgggt ttgtattttt gtgtttgaaa tcaggttatt gcgttttgca aacgatattc
     3481 cggcggcgac ggttcggaat ctgtttgccg gacgggcgcg tatgccgtcc gaaatcgaaa
     3541 ttgaagcggt atggcggatt cgctacaatc cgcgctttgg ttttttcaac atttgaagca
     3601 aggaaacatt atgagcggtc atatgggaaa aggggcggat gcgcctgatt tggtgtacgg
     3661 tttggaggat aggccgccgt tcggtaatgc gctgttgagc gcggttaccc atcttttggc
     3721 gattttcgtg ccgatgatta cgcccgcgct gattgtgggc ggcgcgctgg aactgccggt
     3781 ggagatgacg gcgtatctcg tgtcgatggc gatggttgcg tcgggtgtcg gcacttattt
     3841 gcaggtcaac cgcttcgggc cggtcggttc ggggatgctg tctatccagt cggtgaattt
     3901 ctcgttcgtt accgtcatga ttgcgctggg cgcggggatg aaagagggcg gtttgactaa
     3961 ggatgcgatg atttcgacgc tcttgggcgt atcgtttgtc ggcgcgtttt tggtgtgttt
     4021 ctcggcgtgg cttctgccgt atttgaaaaa agtgattacg ccgacggtca gcggtgtggt
     4081 ggtgatgctg atcggcttga gtttggtaca cgtcggtatt accgatttcg gcggcggctt
     4141 cggcgcaaag gcggacggca cgttcggctc gatggaaaac ttggggctgg catcgctggt
     4201 gctgctgatt gtgctggtgt tcaactgcat gaaaaacccg ctgctgcgga tgagcggcat
     4261 tgcggtcggt ctgattgccg gctatatcgt cgcgctgttt ttgggcaagg tggatttttc
     4321 ggcactgcaa aacctgccgc tggttacgct gcccgtaccg tttaaatatg gttttgcttt
     4381 tgactggcac gcatttattg tggcgggtgc gattttcctg ttgagcgtgt ttgaggcggt
     4441 cggcgatttg accgcaacgg caatggtgtc cgatcagccg attgaaggcg aggaatacac
     4501 caaacgcctg cgcggcggcg tgttggcaga cggcttggtg tcggtgattg cgacggcttt
     4561 gggttcgctg ccgctgacaa cgtttgcgca aaacaacggc gtgattcaga tgaccggcgt
     4621 ggcttcgcgc catgtgggca aatatattgc cgtgattttg gtgctgttgg gtctgttccc
     4681 cgttgtcgga cgcgcgttta cgacgattcc gagtccggtt ttaggcggcg cgatggtttt
     4741 gatgttcggc ttgattgcga ttgcgggtgt gcggattttg gtcagccacg gcatccgcag
     4801 gcgcgaggcg gtgattgcgg cgacttcggt cggcttgggc ttgggcgtgg cgtttgagcc
     4861 ggaagtgttt aaaaacctgc ctgtcttgtt ccaaaactct atttccgccg gcggcattac
     4921 ggcagtcttg ctgaatttgg tcttgcccga agataaaacc gaggcggcgg tcaagtttga
     4981 taccgaccac ttggaacact gattttgaaa acgaatgccg tctgaaacag aatccctgtt
     5041 tcagacggca ttgtttttga ggcttacgct ttttcgtttt ttaatacgcg ctgccggcgg
     5101 gtttcgctta ataccattcc ggcagacacg gagacgttca tgctttcgac cgtgccgaac
     5161 atgggtatgg acaccagcat gtcgcaatgt tcgcgtgtca ggcggcgcat accgtcgcct
     5221 tcgttgccca tcacccatgc cgcgctgtcg ggcagatcgc aatggtaaag gtcggcgttg
     5281 ccgcccatat cggtgccgat gatccaaatg ccgtattctt tcagctcgcg cagggtgcgg
     5341 gcgaggttgg ttacggtgat gtaggggacg gtttccgccg cgccgctggc gactttgctg
     5401 acggtggcgt tcagccccgc gcttttgtct ttcggtgcga tgacggcatg tacgcccatt
     5461 gcgtcggcgg tacgcaggca cgcgccgagg ttgtgcggat cggtgatgcc gtcgagtatc
     5521 agcagcagcg gcggttcgct taagttttcc aatacgtctt cgaggtggac gtggtttttg
     5581 gaggcatcga taaatccgac cacgccctga tggcgcgcac ctttgctgat ggcgttgagg
     5641 cggtcggcat cggcaaaata tacgcggatg ttttcgtttg ccgccttttc caacacttcg
     5701 cgcgtgcgta catcgttttt gccttcttgg acatagagtt cgacgatgga tttggggttt
     5761 tgccacaatc gggcgttgac ggcatggaag ccgtagatag gtctttggtt tgccatatgg
     5821 tgctttgtaa aaagggttca gacggcatta tagcaatttg ccggtatgcc gtctgaaggg
     5881 gttaaaacag gtaggcgatg tatttcacca acaggataaa caagatggat acggcgcagc
     5941 cgattttgaa ggccgttccg acgacaagcc ccaacagcgt acccaagccc gctttacctg
     6001 cctgtatcat gttgcggcag ctgatgagct ctcctgcagc cgcaccgatt agcgggccga
     6061 gtacaaggcc gggaagggag aaaaacatgc cgatgatgct gccgatcaat gcaccgcgaa
     6121 cggcgagctt gcccgctccg gtatattttg ttccccatat acctgccaca tagtccgtca
     6181 gtatgccggc aaggctgatg agtccgacca gccacaggat gcctgcgccg tagatttggt
     6241 atccgccggc ataggcaagc agccatgttc cggcaaacat caatgctaat ccggggaggg
     6301 cggggtaaac gatgcccgcc gtaccgacgg ctatcagggc gagggcgagg atgacgagca
     6361 aggcggtcat aggttcaacc ttttcttttg ttctgaaaaa aacagcttaa tacggagcgg
     6421 cattcttctt gcaggattcc gccccgtatg gcggtgtgcg tattgaggcg tttgtcggca
     6481 aacaggttga cgatgctgcc tgctgcgcca gttttgggtt ctaccgcacc gtatatcacg
     6541 cgcttgattc gtgcctgtat cagtgcggac gcgcacatgg cgcagggttc gagggtgata
     6601 tatatgtcgc atccgtcaag gcggtagttt tgcatttcgc tgcctgcctg tgccaaggcg
     6661 ttgatttcgg cgtgtcggct gacattgcag tcggcaatgc aggtgttgtg tgccgatgcg
     6721 atgattttgc cgtcggaaac gatgactgcc ccgacgggta tttcgccgtc ggcggcggat
     6781 tgttctgctt ggcgcagtgc ttcgcgcatg aagtgttcca tttcttcctg cggcggaaag
     6841 gcggcgacgg gcggatggtt tttcaattcg gcaagaaggc gggctttatg cgcttgcgcc
     6901 atttcttgcg gcggtgtgcc gtccagcagc gactcgagtt gccacagcgt gcttttcgtg
     6961 agggtcaaac ctgatgcctt caataacaag aaggtttgaa ccgcgccggt ttgacgcagt
     7021 tcttcgagcg tacggatacc gagcctgtgt agggcggcga cggttttggg ggcgagcggc
     7081 ggcgtggtca gcatagttta tacaccgaaa aaccgttttg ccgcctcaat cagatgtgtg
     7141 cctgaagtgc cgtctgaaaa cgggtcggca acgcagtcta aaggtgtttt gcgcaaccaa
     7201 gtcagttggc gtttggcaag ttggcgggtg gcggcaatgc ctttctcgac aaatgccggg
     7261 aaatcggttt ttccatccag atatttccat gcctgacggt agccgacgca gcggatggcg
     7321 ggggagtcgg cggtcaggcc gggatagcgg cggcgcaggt tttctacttc gccgataaag
     7381 ccctgttcga gcatcatgtg gaaacgcagg gcgatgtttt catgcaggcg ggcgcggttt
     7441 tcgggaatca gggcggcggt atgcaaatca aaagggagcg tatgggaggt tgggctgccg
     7501 aggtgcgtac tcatcggttt gccggttaaa taataaactt ccaaagcgcg tccgatacgc
     7561 tggccgtcgt tcggtttcag acggcatgct gtttcagggt cgactttttg cagggtgcgg
     7621 tagagaaaat ccaagccgta catttgtttt tgttcgtcca agtcggcacg caggcaggca
     7681 tcggcttcgg gcaaatcgtt caaaccttgg gtcagggcgc ggaaatacat catcgtgccg
     7741 ccgacaatca gcggatattt cccacgcgcc gtgatttcgc caatcagatg cgtgcagtct
     7801 tcgacaaaac gggcggcact gtatgtctga acaggcgtaa tgatgtcgat aaggtggtgc
     7861 ggtacaaagg aacgctcggc atgagacggt ttcgccgtgc cgatgtccat atcgcggtaa
     7921 accagcgcgg aatcgaggct gatgatttcg acaggcaggg tttcggcaat tttgagggcg
     7981 agcgcggttt tgcctccggc ggtcggcccg agcagggcaa aggctttcgg ggtcggcatg
     8041 atgtttcagg gttgcgaaaa tgcggattat agcggaaagc gtgccgacgt tatattttag
     8101 tttgcggaag cacgccgacg gcaagggggc gtgtttaccg tatgcct
//
LOCUS       DAWFBO010000087         8028 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFBO010000087 DAWFBO010000000
VERSION     DAWFBO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.087"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(303..2339)
                     /locus_tag="WGO87_001367"
     CDS             complement(303..2339)
                     /locus_tag="WGO87_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0392721.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     TRTDWANTVERLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVVRPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(2427..3299)
                     /locus_tag="WGO87_001368"
     CDS             complement(2427..3299)
                     /locus_tag="WGO87_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0392722.1"
                     /translation="MEIWNRLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3569..5959
                     /gene="gyrB"
                     /locus_tag="WGO87_001369"
     CDS             3569..5959
                     /gene="gyrB"
                     /locus_tag="WGO87_001369"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0392723.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            6058..7443
                     /locus_tag="WGO87_001370"
     CDS             6058..7443
                     /locus_tag="WGO87_001370"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ0392724.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETKIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7514..7612)
                     /locus_tag="WGO87_001371"
     CDS             complement(7514..7612)
                     /locus_tag="WGO87_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0392725.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         1849 a         2219 c         2233 g         1727 t
ORIGIN      
        1 cctgattttt gttaatccac tataaaatgc cgtctgaagc gttgtttgac cgtttcagac
       61 ggcattttta tcaaattcaa aaatattccg tcattcccgc gaaagcggga atctagaacg
      121 taaaatctaa agaaaccgtt ttatccgata agtttccgca ccgacagacc tggattcccg
      181 cctgcgcggg aatgacggga ttttggctta ctgtttttgt gaaaatggcg aaaccgtgca
      241 aatgctgatg ccgtctgaaa cactgtttga ttgtttcaga cggcattttt tgttcacatc
      301 gatcaagccg cgttgtcgaa accgctgtgg cgcaagagtg cgtctatgcg cggttcgcgt
      361 ccgcggaagg ctttgaagga ttctgccgcg ctgcgcgatc cgccgacggc gaggatttcc
      421 tgccaaaagc gtttgcctgt ggcggcgaca tcgtcgcttt cttcaaaggc ggcgtatgcg
      481 tccgcgctca atacttccgc ccatgcgtag ctgtaatagc ctgcggagta gccgccggcg
      541 aagatgtggc cgaagctgtt ggcgaagcgg ttgtattcgg gcggtcggac gacggcgact
      601 tctttgcgca cgctgtctaa aacctgttgc cagtttttca gacggccttc gtcgtcttcg
      661 ctgtaaatca tcatatcgaa gagggcgaac tccatctggc ggacgaggaa cattccgcgt
      721 tggaagtttt tggcggcgag cattttgtcg aagagttctt tcggcagggg aacgccggtt
      781 tcttcgtggg cggacatttg tgccaagaca ttgtattccc agacgaagtt ttccatgaac
      841 tggctgggca gttcgaccgc gtcccattct acgccgttga tgccggatac gcccagttcg
      901 tccacttggg taagcaggtg gtgcagcccg tgtccggttt cgtggaagag ggtgaggatt
      961 tcgtcgtggc tcaaacgcgc ttctctgccg ccgacgggcg gggcgaagtt gcagacgagg
     1021 taggcggtgg gcagttgcag cgtgccgtct gaaaaacggc ggcgaccttt gtagtcgttc
     1081 atccacgcgc cgccgcgttt gccttcgcgt gcgtacaaat ccatataaac gccgcctatg
     1141 gtttcgccgt tttgttgcaa ttcaaaatag cgcacgtctt tgtgccagac ggggacggtt
     1201 ttttcggtaa atccgatgcc gtaaagtttt ttgatttggg cgaacagtcc gtttaatact
     1261 ttgccgacgg ggaagtattt tttgacttcg gtttcgctga acgcgtattt ggcttcgcgc
     1321 agtttttcgc cagcatagcc caagtcccac ggttgcaaat cggcgagacc gaggctttcg
     1381 cgggcgaagg ctttgacttc ggcgaggtct ttttcggcgt agggtttggc gcggcgggcg
     1441 aggtcgtgca ggaagtttaa aacttgttcg ggggtgtccg ccattttggt tgccagcgac
     1501 aattcggcgt agtttttgaa gccgagcagt ttggcggttt gcagggcgtt tgcgagcgtg
     1561 cggtcgatgt tggcggtgtt gtcgaatttg ccgtcgtctg aaagttcgct ggcgcgggta
     1621 acgtaggcgc ggtagatttg ttcgcgcagt tcgcggttgt cggcgtattg gatgacggcg
     1681 aggtagtgtg gaatctgcaa gccgattttg tagcctgttt tgctttcgct ttgcgcggcg
     1741 gcggcaaaca tggcgagcgc gtcttcggga atgccggcaa gcggtgcggc atcgtcaaag
     1801 taaatgccga acgcgtcggt cgcgtctagg acgttttggg agaatttggc ggaaagttgc
     1861 gcgccttcgg tttgcagttt tgccagttct gcctgctgtt cgggcggcag ttccgcgccg
     1921 ctgaggacga aatcgcgcag atcgtggttg agtttggttt tttgtgcggg ggagagggtg
     1981 tcgaattcgg gggaattttt gatggttttg aagcggttgt acagctcgat gtcttgtccg
     2041 atttcggtga agaagacggt gatttcgggc atcagttcgt tatagacggc gcgcagttcg
     2101 ggcgtgtcgg cgacggagtt gaggtgcgac accacgcccc aaatcctgcc gacgcgctcg
     2161 gtgatgccgg tcagacgctc gacggtgttc gcccagtcgg tgcgcgtttg ggctttgatg
     2221 gcggcgattt gttcgcgcgc ttcggcgatg gcggtttgca gggcgggttt gatgtcttcg
     2281 gttttgattt ggtcgaaacg gggttcttcg cccaagtgga gcagtgcgtt gtcagtcata
     2341 agatgggttt cctttgcgtg ttgtgcttca gacagcatat cggcaatatg ccgtctgaag
     2401 ccggaaatgg ggtcggtcag ggaaagttaa agtgtttcgg agccggcggg gtaatttaac
     2461 aggcggtatt gtacgcgcaa aaattcgtcc ataaccgcct gttggatttc cagccgcttt
     2521 gaaacggggg aggcgaagcg gacgatgatg cggtatgcct tgtcatcgta cggcacgcgg
     2581 gtaacgcgcg gtctggcggc gggcgtgata aacagttttt ccgcctgcac gttttccaaa
     2641 tgccgttgga tggcggggat gtagggcgcg cacaagggct cgagtacggc tttcagacgg
     2701 catacggctt catccgaatc caaatggatg ggaacgggga tttcgaccgt atggatgaca
     2761 tagtcgccca aaatattgtc gcggcgcacg gggtggctca acaacaggct gttggggaaa
     2821 gaaacggtgg ttcccgcaag ctgtccgacc agggggttcg gaccgacctg catcatcagc
     2881 gtgttcaaca ggttgatgtc gaccacgcgc ccgcgcaggc cgttgatttc gatatagtcg
     2941 ccgaccgagt attgctgggt ggcagacctt aaaatactgc ccgacagaca cataatcagt
     3001 tcctttgtcg ccacgacgac cgccgccgcc accgcaaaca tcgacaaagc cagcgtttgg
     3061 atttgcgccg accagataaa tgccagcgaa aacagcacca aaagcagcgt tatattgcgg
     3121 ctggcaacca aaaaccgccg cttgctttcg atgccgaaat ccggatgccg tttgaagtgg
     3181 atattcaaca gaagggcgcg cgccagcagc aaagccgcaa ccgccgccac ggattcgacc
     3241 gcctccgcac gtatcgggac ggcaccgagc caagtgtcca acctattcca tatttccatt
     3301 tccgcgcccc tgtccgaaaa gtatagggtg tagattttag tggcaaaaaa acgcttttgc
     3361 cactttacgg gacaatcccc gacctgatgc cggaggcaaa cgtaccgttt catcttttcc
     3421 cggcggcaca ctgatgcgga taacgctgaa ttaggcggca aaatcggcta taatacacaa
     3481 ttatatatgt cgtcccgccc gctttttcta caaggtgcgg acttgttttg gcggcatcgc
     3541 aaaatctttt caaaatccgg caaaaaatat gactgaacaa aaacacgaag aatacggcgc
     3601 cgacagcatt caggtgctcg aaggcttgga agcggtacgc aaacgccccg gcatgtacat
     3661 cggcgacacg caggacggca gcggcctgca ccatatggtg tttgaagtat tggacaacgc
     3721 catcgacgaa gcgctcgccg gacattgcga caaaatcacc gtaaccatcc atgccgacca
     3781 ttccgtcagc gtggccgaca acggccgtgg catgccgacc ggcatccacc cgaaagaagg
     3841 ccgctccgcc gccgaagtca tcatgaccgt cttgcacgca ggcggtaaat tcgacaacaa
     3901 cagctacaaa atctccggcg gcttgcacgg cgtgggcgta tccgtcgtca acgcgctgtc
     3961 cgactgggtt acgctgacca tctaccgcga cggcaaagaa cacttcgtcc gcttcgtgcg
     4021 cggcgaaacc gaagagccgt tgaaagttgt cggcgattcc gacaagaaag gcacgaccgt
     4081 acgcttcctc gccagcacgg aaactttcgg caacgtcgaa tacagcttcg acatcctcgc
     4141 caaacgcatc cgcgaacttt cgttcctgaa caacggcgtg gacatcgaat tgaccgacga
     4201 gcgcgacggc aaacacgaaa gcttcgccct ttccggcggc gtggctggat tcgtgcaata
     4261 catgaaccgc aaaaaaacgc ccttgcacga aaaaatcttc tacgcgttcg gcgagaaaga
     4321 cggcatgagc gtcgaatgcg caatgcaatg gaacgacagc tatcaggaaa gcgtgcagtg
     4381 cttcaccaac aacatccctc agcgcgacgg cggtacgcac ctgaccgcgt tgcgccaagt
     4441 gatgacgcgc accatcaaca gctacatcga agccaacgaa gtcgccaaaa aagccaaagt
     4501 ggaaaccgcc ggcgacgata tgcgcgaagg tttgacctgc gtgttgtccg tcaaactgcc
     4561 cgaccccaaa ttttcgtccc aaaccaaaga caaactggtt tccggcgaaa tcggtcccgt
     4621 cgtcaacgaa gtcatcagcc aagccctgac cgacttcctc gaagaaaacc ctgccgaagc
     4681 caaaatcatc accggcaaaa tcgtcgatgc cgcccgcgcg cgcgaagccg cccgcaaagc
     4741 ccgcgaaatc acccgccgca aaggcgtaat ggacggcttg gggctacccg gtaaactcgc
     4801 cgactgccaa gaaaaagacc ccgcattgtc agaactttat ctggtcgagg gcgattctgc
     4861 aggcggttcc gccatgcagg ggcgcgaccg caaattccaa gcgattttgc cgcttaaggg
     4921 caagattttg aacgtcgaaa aagcgcgctt tgaaaaaatg ctcgccagtc aggaagtagc
     4981 cgctttgatt accgcgttgg gcgcgggcat cggcaaagaa gaattcaatg ccgagaagct
     5041 gcgttaccac cgcatcatta tcatgaccga tgccgatgtg gacggcgcgc atatccgcac
     5101 cttgcttttg accttcttct accgccaaat gcccgaactg gtcgagcgcg gctacatcta
     5161 tatcgcccag ccgcctttgt ataaagcgaa atacggcaaa caggaacgtt acctcaagga
     5221 cgagttggaa aaagaccaat ggctgctcgg tcttgccttg gaaaaagcca aaatcatttc
     5281 agacggccgc accatcgaag gcgcagaact tgccgacacc gccaaacaat tcctgttggc
     5341 aaaaaccgtc atcgaacagg aaagccgctt cgtagacgaa ctcgtcctgc gcgccatgct
     5401 acacgcgtcg cccattgatt tgacgtcgtc tgaaaacgcc gataaagccg ttgccgaact
     5461 ttccggtctg cttgacgaaa aagaagtcgc cctcgaacgc atcgaaggtc atgaaggaca
     5521 ccggttcatc aaaatcacgc gcaagctgca cggcaacgtc atggtcagct acatcgaacc
     5581 caagttcctc aacagcaaag cctaccaaac cctcacccaa accgccgccg cgctcaaagg
     5641 catggtcggc gagggtgcca agctttacaa aggcgaaaac gggtacgacg cggacagctt
     5701 tgaaaccgct ttggacatct tgatgagcgt tgcccaaaaa ggtatgtcca tccaacgata
     5761 caaaggcttg ggcgagatga accccgagca gctgtgggaa accacgatgg atcccgccgt
     5821 gcgccgcctg ttgaaagtgc gcatcgaaga tgccattgcc gccgacgaag tgttcgttac
     5881 gctgatgggc gacgaggtcg agccgcgccg tgcctttatc gaaaacaacg cgctgattgc
     5941 ccaaaatatc gacgcataag tgccgtttta aaaaaggaga cgggcatcgt gccgcgtctc
     6001 cttttggttt gtcaaacgga agccatgccg tctgaaaccg cctttcggag caaaaccatg
     6061 gtcagcattt ttgatatttt caaaatcggt atcgggcctt ccagttcgca tacggtcggc
     6121 ccgatgaagg cagccgccgc ctttgcggca ggtttggatg cacaggctgt tcgcatcgtc
     6181 atcgacattt acggctcgct cgccctgacc ggatacggac acggtacatt tgacgcgctg
     6241 atgctcggtt tggaaggcag cctgccgcac gacatcccgc ttgtcggcat tcccgaacgc
     6301 ctcgaacgca tccgcacgca gcacatcctc cggctcaacg ggcaagaaat ccgcttcatc
     6361 cccgaccgcg acctgaacat actcggcaat caagtgctgc ccaaacaccc caacggcctg
     6421 cgttttaccg cctatgcttc agacggcacg gtattgaatg aacaggttta ttattcggtc
     6481 ggcggcggct ttgtcgttac cgaagaagat tttgaccggc aggcggaaac ggaaaaagcc
     6541 gttccctatc cctataccag ttgcgccgaa ctgcttgccc gatgccgtct gaaccggctc
     6601 gacatctccg aagtcgtgtt ggcaaacgaa gccgcgcttg ccggatgcgg cgaagccgaa
     6661 atccgccgcc gcgccgctgc cgttgccgag gttatggaag gctgcatcaa acgcggcttg
     6721 ggtgcggacg gcgaactgcc cggcggattg aacgtccgcc gccgcgcccc gcagcttgcc
     6781 gccaagctca aagtcctgcg cgaaaccaaa atcgtcaaca cccagctctg gccgatggtg
     6841 tacgccatgg cggtcaacga agaaaacgcc gccggcggac gcgtcgttac cgcaccgacc
     6901 aacggcgcgg caggcatcat tcccgccgta ttgcactatt tccgcaagtt caatccgcac
     6961 gccacacagg aacgcgtcga aaacttcctg ctcaccgcag gcgcaatcgg catcctctac
     7021 aagaccaacg cctccatttc cggtgcggat gtcggctgtc agggcgaagt cggcgtagcg
     7081 tgttcgatgg cggcgggcgc atacgccgaa gtcatcggcg gcacgcccaa acaagtggaa
     7141 aacgccgccg aaatggcgat ggaacaccat ttggggctga cttgcgaccc cgtcggcgga
     7201 ctggtgcaaa tcccctgcat cgagcgcaac ggcatcgccg ccgaaaaagc cctcaaactc
     7261 ggcaccctcg cgctcttgga agacggcacg gacaaaaaag tctcgctcga cgaagtcatc
     7321 cgaaccatgc tgcaaaccgg ccgggatatg aaggcgacct acaaagaaac ctcgcttgcc
     7381 ggactcgccg ccacgctccg gaaaaaagcc gtccccgtat ccgtgcgcgt ggtcgagtgc
     7441 tgaggatgga cagaaacgaa aatgccgtct gaaaacagtt ttttagacgg catttcattt
     7501 atttcttgcg gctttaccgt ttcagtgcca gcgtcaacac gcccgccgta accagcccca
     7561 agcctatcca ttcctgcgtg ttcgggcgtt cgtctaagaa aaccaccgcc atcaggaatg
     7621 caaaccggcg ttgtttgcag catccggcaa gaatttttaa aaggtctttt ctaaaatatg
     7681 cagatattcg aatgtcgaat ttgccttatg attacggttt tctgtcttat ctgccttaaa
     7741 ccgccggtat tccgtttgaa ccaaatcata tttgccgaaa cgctcaaaaa tctcccttac
     7801 ctgcccgaca gacatcaagc cttcgttgtt gtagctgaga aaatatattg aaagtccgca
     7861 tccctaatca aagcctcaaa taccggaatc acatgcgatt ttgagcaagt ccgcgctaaa
     7921 acacatcgta cagcgcaaaa ccggatggga atggcaaaat ccggaaacaa acatttttca
     7981 aagcatacaa acaaaaaaat accaaccgga ggttggtatt ccttaact
//
LOCUS       DAWFBO010000088         8020 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFBO010000088 DAWFBO010000000
VERSION     DAWFBO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.088"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(68..814)
                     /locus_tag="WGO87_001372"
     CDS             complement(68..814)
                     /locus_tag="WGO87_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0392726.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WGO87_001373"
     CDS             complement(993..1664)
                     /locus_tag="WGO87_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0392727.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WGO87_001374"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WGO87_001374"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0392728.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLMMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WGO87_001375"
     CDS             complement(2481..2963)
                     /locus_tag="WGO87_001375"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0392729.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WGO87_001376"
     CDS             complement(2995..3684)
                     /locus_tag="WGO87_001376"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0392730.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPIADTLKCADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIHPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WGO87_001377"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WGO87_001377"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0392731.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVAHTETKRSGKLKVIRADENELAEHG
                     RYLDGLGEACIWRKTQMPSENGGTEA"
     gene            complement(4571..5881)
                     /locus_tag="WGO87_001378"
     CDS             complement(4571..5881)
                     /locus_tag="WGO87_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0392732.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WGO87_001379"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WGO87_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0392733.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WGO87_001380"
     CDS             complement(6056..6355)
                     /locus_tag="WGO87_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392734.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="WGO87_001381"
     CDS             6706..7902
                     /locus_tag="WGO87_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0392735.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAAVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1886 a         2405 c         1958 g         1771 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcac gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatctctga accggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tattccgttt tcgagtacgc cggtgatttt gttgatttcg tcttccatca
     1861 tcagcggttt gtcgatattc aagccgtgga catcgacgat ttggttgccg taaaaagtgg
     1921 tgtagccgat acgcaattca ggctgtccgc ccatagcgag cagtttgcgc gaaacaaggg
     1981 aacgcgcgct ttcgacgact tccacgggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcgtc cgcaatgcag acgaattttt ctgatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatccgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggtggat gcccaatttt tcgacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3241 gtccgcgcac ttgagcgtat cggcaatggg aattgccaaa atcccacctt cggcagcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcaac gcgcggcatc
     3361 gtgtaccaga atattgtcgg tttccgccgc caaaccggtt tccaacagtt ttgccacacc
     3421 gttgcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaag cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatac tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagggcgata tttttgcgtt tcatgcctcc gtcccgccgt tttcagacgg catctgcgtc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatc gcccgtgctc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcgccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaattg ccgccgcgtc
     3901 atcgctaaat aaacttcgcc caaaagctcg cagtcgatca gcgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca gaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tttctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgctg ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgata gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcacgca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg ggtatcggca gccttggcgg
     5281 gtacggacgg cataaacagg ctgccgaccg tgcctcctac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat acccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgttagaat agcctgcttc ttttatcaac cgtttcagac
     6601 ggccccacta ctttcccgcc taggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaataaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 gccgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac taacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aaggttttat ccgcacacga
     7921 actgcctccg gaaatggagg cagttttttt atccggcttt ccatgcttaa acagcactgc
     7981 ctcttttcag acattgacgg ttgcagccgc ttacctgaac
//
LOCUS       DAWFBO010000089         7983 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFBO010000089 DAWFBO010000000
VERSION     DAWFBO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.089"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WGO87_001382"
     CDS             complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WGO87_001382"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0392736.1"
                     /translation="MSDNRLDAARRHSLFLARNLDSGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDAIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRDTKSPQHLSVVAMGKAGGYELNVSSDIDLIFTYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGNSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAELGIMP
                     SENVETLLAAYRFLRDIEHRLQYWDDQQTQTLPTSPEQQQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLANYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWRDVFGEEAATA"
     gene            complement(3151..3354)
                     /locus_tag="WGO87_001383"
     CDS             complement(3151..3354)
                     /locus_tag="WGO87_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392737.1"
                     /translation="MGFWNGVAKAVGEGMIEAANEHKALKMEYAEKSSEELHEIVKSD
                     GFFKNSTREKGAAYAVLKERGEV"
     gene            complement(3491..3964)
                     /locus_tag="WGO87_001384"
     CDS             complement(3491..3964)
                     /locus_tag="WGO87_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0392738.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4017..4298)
                     /locus_tag="WGO87_001385"
     CDS             complement(4017..4298)
                     /locus_tag="WGO87_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392739.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4385..4708)
                     /locus_tag="WGO87_001386"
     CDS             complement(4385..4708)
                     /locus_tag="WGO87_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392740.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5041..6048)
                     /locus_tag="WGO87_001387"
     CDS             complement(5041..6048)
                     /locus_tag="WGO87_001387"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0392741.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGKDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6280..6516
                     /gene="acpP"
                     /locus_tag="WGO87_001388"
     CDS             6280..6516
                     /gene="acpP"
                     /locus_tag="WGO87_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0392742.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6672..7919
                     /gene="fabF"
                     /locus_tag="WGO87_001389"
     CDS             6672..7919
                     /gene="fabF"
                     /locus_tag="WGO87_001389"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0392743.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1842 a         2120 c         2069 g         1952 t
ORIGIN      
        1 tagaatgcgc tattttaatg gaaacggcgg ggttttaaac ggttcttatt tgtttgtttt
       61 gatttttgct tgggtaaaaa atcccatcat tcccacaaaa acagaagcct gaaatcccgt
      121 cattcccgcg aaagaggaat ccggtttttg ggtttcagcc attcccgata aatcgcttta
      181 gctctgccgc aaccttaaaa cggcacggta cgaaaatacc gtctgaaacc cagattgtca
      241 ggcttcagac ggcatttttt gttcaggcgg ttgccgcttc ttcgccgaac acgtcccgcc
      301 acaatttcct gacattgccg taatgtgcca acagttcgcc ggttacttcg gtttttgccg
      361 cgtcgcgcag tttggtgttg tgctgctgcc ggcggtagaa gcggtaggcg gtgcggcttt
      421 gttcggcgag gtttttgtcg ataaggctgc agtcggcggc gatattcaag agggcgatgt
      481 tgccgtagtt ggcgagaagt tgcggatatt ttcgggcatg ggcaaggatg aggtattgga
      541 cgataaattc gacgtcgacc acgccgccgc gtgcgtattt gacgttgctg tcggcaggcg
      601 gatgggtggg gaacattttt tcgcgcattt cgatgatttc gcctgacaag gcggtttggt
      661 cgcgttcggc ggtgaggatt tcggtgcgga tgcggtcgaa ggccgtctga atttcgggcg
      721 tgccgcagat gaagcgggcg cgggtaaggg attggtgttc ccacgtccag gcgttttcgc
      781 gctggtattt ttcaaaggcc gcgatgctgt gggcgaggaa accggcgtcg ccattagggc
      841 gcaggcgcag gtcggtttcg tagaggctgc ctgcgccggt ggcggcggaa agccagttgg
      901 tcaggcgacg ggcgaggcgg ctgtacacgt cgcctgcgtc ggggtgggga tcgtcgtaga
      961 gatagacgag gtcgaggtcg gaggcgtagc cgagttcttt gccgccgagt ttgccgtaac
     1021 cgacgacggc gaattgcggg gtgtcgcggt gttttttggg catgtccgcc cacgcgcaca
     1081 gcagggcggc ggcgaggatg gtgtcggcga gggcggagag ttggtcggag agggattcta
     1141 ccgtccacag tccggcgagg tcttggacgg cgagacggaa gacttgggcg tgctggaagc
     1201 ggcgcagggt gtccatttgc gcttcagtat cgccgccgca ggctttgagg tcgtctgaaa
     1261 gggcggcggc gagtgcctgc caatcaaacg cggtatccaa aagctgcgcg ctgatgagtt
     1321 cgtccaacaa aatcggatat ttgttcagat acgccgccac ccaagaactt tggcccataa
     1381 tctctgccag ttgcgccaag gtttgcggat gttcgttgag gaaggcgaga taggcggatc
     1441 ggcggctgat gttttcgaga aaatccaaca gccgcatcaa tgtatcggtc gggttgcttt
     1501 gctctgccgc cgcctgtacg aacagcggca caaccgcatc gaaacgcggc tgggcgtgtg
     1561 cggaaagatg gcggtattta tggccgtggc ggatttggtc gagccttgcg gcgacggttt
     1621 cggcatcgaa cccgtgcgcc ttcagacggc atcgccgccc ttcttcgtcg ggcttgtcct
     1681 gccatgccca ttgccattcg ctgttgtctt gcgtttgctc ttcgggttcg ctcaaaattt
     1741 cgttgaacaa ctgattgact ttgttccgat gaacattgag accgtctgaa aaagcggcat
     1801 agctgtcgaa gcccatgctt tcggcaagca gttgctgctg ttcgggcgag gtcggcaggg
     1861 tttgggtttg ctggtcgtcc cagtattgca ggcggtgttc gatgtcgcgt aggaagcggt
     1921 aggcggcaag cagggtttcg acattttcag acggcatgat gcccagctcg gcaagttttt
     1981 tcagcgtttc ctgcgtgcct ttcagttgca gcgcgcgcat ttgtccgccg cgtatcatct
     2041 ggaaaatctg ggcgataaac tcaacttcgc ggatgccgcc cgcgccgagt ttgatgttgt
     2101 ccgccatgcc ttttttgctg acttcgctgc ggatttggcg gtgtaggttc cgcatcgcct
     2161 catacgcgcc gtaatccaga tatttgcgga acacgaaggg gcgcaccagc gatttgatgt
     2221 cgttcggata cggcgtaacc acacggcctt tgcaccacgc gtagcgttcc cattctcgcc
     2281 cctgtgtaat caaatattgc tccagcgcgg tttcgctcaa taccaacgcg cccgaattac
     2341 cgtccggccg cagccgcata tcgacgcgga acacctgtcc gtcggcggtg atgtcgttca
     2401 gcagcgcaat cagtttctgc ccgactttgg tgaaaaactc ttgattgccc cgttcgcgcc
     2461 tgccgtcggt gtcgcccgat tcgggatagg tgaaaatcaa atcgatgtcg gaagacacgt
     2521 tcaactcata gccgcccgcc ttgcccatcg ccaccacgct caaatgctgc ggcgatttgg
     2581 tatcacgccc gatcggcgtg ccgtacatgt cccgataata ggcgtaggca aaatccagcg
     2641 cggtattgac ggcaaaatcg gcaaacagcg taatcgtgcg ggttacttcg ttcaaatcgc
     2701 tgatacggtt gatatcgcgc acgataatct gcgacaccac ataacggcgc aacccgcgca
     2761 actgccgcgc caactcttcc tcgttttctt ccgcacggat tgcgtcccag tcggcaaagg
     2821 cttggaaatc cgcttcggtc aacaccttgt ccaacatcgg caggaaaatt tcgggcttga
     2881 gtttgccgga atcaaggttg cgggcgagaa acagggaatg gcggcgggcg gcgtcgaggc
     2941 ggttgtcgga catttcagat tccgtttgga aggatgacgg gaagatgatg gcggattcac
     3001 ttcaaatcag gacagggcga ggggatgccg taccggttta aagtcaagcc actatatcat
     3061 agcaaccttc gatgccgtct gaaaccttac cccgacatcc agacggatgc ctgccgataa
     3121 aaaacagcgg caaatgccgc cgtttcctgt tcacacctcg ccacgctctt ttaaaacagc
     3181 ataagccgca cctttctccc gtgtggaatt tttaaaaaaa ccatcactct ttacgatttc
     3241 atgcagctcc tcacttgatt tctccgcata ttccattttc aacgccttat gctcattggc
     3301 tgcttcaatc attccctcgc ctactgcttt tgccacacca ttccaaaaac ccataatcat
     3361 gtcctccaat cgtttcaaat aaataaggtc gttgtttctg tcaagaaaca acgacagttt
     3421 ataaactgtc ggaaaacaaa atgccgtctg aaggccggat aatgcttcag acggcatcgg
     3481 attgccggtt ttaaccctgc cctccgccgt tgttcaaata gtgttcccag tgagaatata
     3541 accgggcaat attttcttca taggtgtggg cagaaggaat ataaccgtct ttgggcggca
     3601 tatacctgac aaatgattct atttcttccg gataatcaaa atctgcataa attttttcaa
     3661 cttcccccaa aggatcctca aattcttctt ttctatggaa tacatcgctt aagaggacgt
     3721 acagccaatt tttaggggaa taatccctca tttccgaagc caaatcttcc agaacaggct
     3781 ttaattcaag aatattggat ttgttgatta aagatagttt gaacacacgt tcatcatgat
     3841 ctgaaagcgt catttttcgg gcataagctg ccacatcagc ccaacccaag tatttattcc
     3901 cataccccca cagaatatcc ttccaagaaa gacggacttt ttgcgcggtt aaatcaaggt
     3961 tcatttttta ctccgattgc ctgccccgtc attccgcccc ttccgattga cgcctgttac
     4021 gaaaagtctt ccaaatgacg gctgattgcc tcttcaacag tcggatgcca atccataaat
     4081 gaagcctgca attcctctaa aaccaactca ttgaataaat catccaaatt ccgccaatcc
     4141 gttttttcat ccggatagat gaaatttgcc ataacgacag tatgtttgtt ttgggaataa
     4201 tccgataagg cgcaaatcat cagataccgc cccgaatgct ccagataaaa aattcgtttt
     4261 tccaaaggaa accggttatt ccgttgcccg ttttccattt ttcatccttt ttaacgtatc
     4321 ggcttccgcg tccttcaaat tgagttacct gccttccgcc aagcaaacgc ccgcgcccct
     4381 ttcatcattt gaatatccgt ttgaccatat ttaccagata actatcttct ttatataaat
     4441 caatcagcaa aggcacgata cccggctcat aaaataattc tgtcgtatca gatgtttctt
     4501 ttaacgcgga aattatctcc aacagttcat acccgtatcg gggataagca aacaaatagg
     4561 ggactgcttc ccgcaaacat gaaaatgctt cgtaactttg catttcccat atatgttcat
     4621 ctttaaaatc actcaaatac cattcatcca attcactttc cggatcggat atcaatcgga
     4681 taaattcatt tagttcgtcc ttaaacatat caaatcctta aatataaaat cttttgatta
     4741 tccgccgtat ccttattttc cacatcatga atttcaacgc cgttcatatt ttgcgttttc
     4801 ccaagccgtc cgccacagta taaaattatt ttttgtatca attgcttata tatttaacaa
     4861 cttaccgccg gacttgcttt ggctgcgtat caagattgtc cggttcgcgg cgggcgtttg
     4921 ctttgtttca aacagggcgg cgcgtaaggg attttcaagg ggcggcaggc ggaaaaagga
     4981 aatgaaaatg ccgtctgaac ggcaaaacgt gcgtttagac ggcattttgg gcgggacgcg
     5041 tcatcgcgcc aaagccttca aacattcttt gaccaatgcc ggacctttgt atatcaatcc
     5101 gctgtacact tggacggcgg tcgcgcccaa gcggatttta tctgccgcat ccttgccttc
     5161 cataatgccg cctacgccga taatcggcag cttgccgtcg atgtggtctg ccaacagctt
     5221 caacacccga ttactttttt catgaacggg cagcccgctc aaaccgccct gctcgcctgc
     5281 gagcggatgg ctgccgagac ttgatttgtc gatggtggta ttggtagcga tgatgccgtc
     5341 catttcgacg gatttgacaa cgtgggcgat gtcttcgatt tgtgcttcat ccaaatcggg
     5401 ggcgattttg acggcgagcg gaacgtattt cccgtataca gaggcaagct gtgcctgttt
     5461 gtttttcaaa gcctcaagca atgcgctcaa ctcgtcgcca ccttgcagcg cgcggaggtt
     5521 tttggtgttg ggcgaggaaa tattgacggt aatgtaactt gcgtgtgcgt aggctttttc
     5581 aaggcagatt aaataatcat cggcagcgtt ttcgatgggt gtaaccgcgt ttttaccgat
     5641 gttgatgccc aatacgccac tgaatttact tttttcgatg ttgcgtatca tggtgtcgat
     5701 accgtggttg ttgaaaccca tgcggttgat gatgccttgg tgttcgggaa cgcggaagag
     5761 gcgcggctgc gggttgccgg gctgcggttt gggcgttacc gtgccgattt cgatgaaacc
     5821 aaagccgagc gcgcccaatg cgtcgatgta ttcgccgttt ttgtcgagtc cggcggcaag
     5881 tccgacaggg ttgggcaaat ccatacccat caattttaca ggtttggtac ggttgccggt
     5941 tacgggaatc agacccaatt tataaaccgt gtagagcgcg tcgagcgtga agtggtgggc
     6001 tttttcggca tcgagtgcaa acaggatgcg gcgggcaagt ggatacatga cagggctttc
     6061 tgcttcagat ggaaatgccg ccattttaac cgaaatcccg ccaaacatac ggcacacagg
     6121 tcaaacttga acgacaaaac ccgccgcacc caaaaatgta caaatcggca aatattgata
     6181 taatagtcat tagatagaaa actctattaa aagtcggtac aataccaccg atttatttcc
     6241 cttaaacaac ctgccgcaag gcataaagga acgactgata tgtcaaacat cgaacaacaa
     6301 gttaaaaaaa ttgttgctga acaactgggc gtaaacgaag ccgacgtgaa aaacgaatct
     6361 tctttccaag acgacttggg cgcggattct ttggacaccg tggagctggt tatggctttg
     6421 gaagaagcat tcggctgcga aatccccgac gaagatgccg aaaaaatcac caccgtccaa
     6481 ctggctatcg actacatcaa tgcccacaac ggctaaccgg tcgtcgcccg acacaacagc
     6541 ctctgctgca ccgcgcaata gaggcttttc ccttatatgg caaactgttt gaacccctgc
     6601 cgcaaattac agacggcatc tgcacgacag ccgcatatgc ccccattccg acaaccaaca
     6661 gcgaggttat catgagtcag agaagagtag tcattacagg cttaggtcag gtttcccctg
     6721 tcggcaacac tgtcgcagag gcttgggaca ccctgctcgc tggcaaaagc ggcatcggcg
     6781 cgattacccg ctttgacgca tccgacatca acagccgtgt cgccggcgaa gtgcgcggtt
     6841 tcgacatcgg acaatacatc agcgcgaaag aagcgcgccg gatggacgta ttcatccact
     6901 acggcattgc cgccgcattg caggcaatcg ccgattcggg cttggacgat gtggaaaacc
     6961 tcgacaaaga ccgcatcggc gtgaacatcg gttccggcat cggcggactg cccagcatcg
     7021 aggtcaccgg caaagccgta atcgaaggcg gcgcgcgcaa aatcaaccct ttctttatcc
     7081 ccggttcgct gatcaacctg atttccggac acgttaccat cctcaaaggc taccgcggcc
     7141 cgagctacgg gatggtttcc gcctgtacca ccggcgcgca cgccatcggc aattccgccc
     7201 gactgatcaa atacggcgac gcggacataa tggttgccgg cggcgcggaa ggcgcaatca
     7261 gcactttggg cgtgggcggt tttgccgcga tgaaagccct ctccacccgc aacgacgacc
     7321 ccgccaccgc ttcccgtccg tgggacaaag gccgcgacgg cttcgttatc ggcgaaggcg
     7381 cgggcatatt ggtgttggaa gaattggaac acgccaaaaa acgcggcgcg aaaatctacg
     7441 ccgaaatcgt cggcttcggc atgagttccg atgcttacca tatcaccgcg ccgaacgaag
     7501 aaggccccgc ccttgccgtt acccgcgcgc tgaaagatgc cggcatcaat cccgaagacg
     7561 tggattacgt caacgcgcac ggcacgtcca cccccttggg cgatgccaac gaaaccaaag
     7621 ccctcaaacg cgcgttcggc gaacacgcct acaaaaccgt cgtcagctcg accaaatcca
     7681 tgaccggcca cctgctcggc gcggcgggcg gcgtggaggc cgtgtacagc attttggcga
     7741 tacacgacgg caaaatcccg ccgaccatca acatttttga acaagacgtt gaagccggct
     7801 gcgatttgga ctactgcgcc aacgaagcgc gcgacgcgga aatcgacgtt gccatttcca
     7861 actccttcgg cttcggcggc accaacggca cgctggtctt caaacgcttc aaaggctgat
     7921 tccgcaaagc cgccgccgac atcgaaatgc cgtctgaaac cgtttcagac ggcattttta
     7981 tag
//
LOCUS       DAWFBO010000090         7881 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFBO010000090 DAWFBO010000000
VERSION     DAWFBO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.090"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..140)
                     /locus_tag="WGO87_001390"
     CDS             complement(<1..140)
                     /locus_tag="WGO87_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0392744.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="WGO87_001391"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="WGO87_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0392745.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="WGO87_001392"
     CDS             1619..2668
                     /locus_tag="WGO87_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0392746.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /locus_tag="WGO87_001393"
     CDS             3356..4777
                     /locus_tag="WGO87_001393"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0392747.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEADDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4860..5639
                     /gene="xth"
                     /locus_tag="WGO87_001394"
     CDS             4860..5639
                     /gene="xth"
                     /locus_tag="WGO87_001394"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0392748.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5626..5970
                     /locus_tag="WGO87_001395"
     CDS             5626..5970
                     /locus_tag="WGO87_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392749.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5981..6511
                     /locus_tag="WGO87_001396"
     CDS             5981..6511
                     /locus_tag="WGO87_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0392750.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6740..7855)
                     /gene="asd"
                     /locus_tag="WGO87_001397"
     CDS             complement(6740..7855)
                     /gene="asd"
                     /locus_tag="WGO87_001397"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0392751.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2069 a         1951 c         2037 g         1824 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
       61 gcacgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcagacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggt agcgtctggg cggaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgttaaa cgctaccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 accctcatcc gcgacctcga caccgacgaa atcgttgccg acctcactta ccacggacag
      721 cgcgtctgcc tagccaaagg cggcaaaggc ggcttgggca acatccactt caaatcctcc
      781 gtcaaccgcg ccccaaaaca atccacgccc ggcgaagaag gcgaagcccg ttccctgcaa
      841 ctcgaactca aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccaa acttaggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg cgcggcagaa ggcgcgggtt tggggcatcg tttcctcaaa
     1081 cacttatcgc gcaccggatt attgctgcac gtcgtcgatt tggcgccctt cgacgaaacc
     1141 gtcaaccctg ccgaagaagc ccttgctatc atcaacgaat tgcgcaaata cgatgaagaa
     1201 ctttacggca aaccgcgctg gctggtgctg aataaactcg acatgcttga cgaagaagaa
     1261 gcccaaacgc gcactgccgc cttcctcgaa gccgtcggct gggactaccc caaacccgac
     1321 gaccgtttcc aatttgacat ggaaaccccg cgcctcttcc aaatcagcgc gctgacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgaccgagaa aaaacgtatc
     1441 gaagctgaaa aagccgaagc ggagaaagca gcggcaaatg ttgaaattat cgaacagcag
     1501 cctaaaacgg atacgggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggc aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatgcctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggcggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctt tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgacgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgag tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagcaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctagt ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcagccata ttaataatcc gcaactgcgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga ttaatccata atcccatgac
     3361 caccatctac aacaccctta cccgccaaaa agaacccttt gcccccatcg accctgaaaa
     3421 cgtgcgtatg tacgtttgcg gcatgactgt ttacgattat tgccacttag gccatgcccg
     3481 tgtgatggtt gtgttcgaca tgattgcccg ttggttgcgt gagtgcggct atccgctcac
     3541 ttatgtacgc aacatcaccg acatcgacga taaaatcatt gcccgtgcgg ctgaaaacgg
     3601 cgagaccatc ggcgagctga ctgcgcgttt cattcaggct atgcacgaag atgccgatgc
     3661 tttgggtgtg ttgcgtcccg atatcgagcc gaaggcgacg gaaaacattc cgcaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa atggcgacgt
     3781 ttactacgcc gtgcgtgagt ttgccgctta cggacaactg tcgggcaaat cgttggacga
     3841 cctgcgcgcg ggcgaacgcg tggaagtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 attgtggaaa gcggcgaaag caggcgaacc ggcgtgggaa agcccgtggg gcaacggccg
     3961 tccgggttgg cacattgaat gctctgccat gagtgaaaac ctgttcggcg ataccttcga
     4021 catccacggc ggcggcgcgg atttgcaatt cccgcatcat gaaaacgaaa tcgcccaaag
     4081 cgtcggtgcc agcggtcata cctgcggcca cgaccacgca caaacccacc acggtcaaag
     4141 cattgccagc cacgtcaaat attggctgca caacggcttt atccgcgtgg acggcgaaaa
     4201 aatgtccaaa tcgttgggca acttcttcac tatccgcgaa gtgttgaaac aatacgaccc
     4261 cgaagtcgtg cgcttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gacgacgcaa aaggcgcgtt gacccgtcta tacaccacct tgaaaaatac
     4381 gccacccgcc gatccgatgc cgtctgaagc cgacgatgac tacacgcgcc gcttttatgt
     4441 tgcgatgaat gatgattttg atacggtgaa agcggtggcg gtgttgttcg aacttgccgg
     4501 cgaagtgaac aaaaccaatg acgcgcaact ggcaggctgc ctgaaagcct tgggcggcat
     4561 catcggactg ctgcaacgcg acccgaccga gttcctgcaa ggcggcgcgg cttcagacgg
     4621 cctctccaac gaagaaatcg aagacttgat tgcccggcgg aaacaggcgc gctccgataa
     4681 aaactgggcg gaatccgacc gcattcgcga ccttctgaac gaacacaaaa tcattttgga
     4741 agacaacgcc ggcggcacga cttggcggcg cggttgaaat ccatcgaaaa gggtaaatgt
     4801 gccgcatttt gccctttttt gttcagacgg catttttatt gtgagaactt atttttatta
     4861 tgcttaaaat catttccgcc aacgtcaacg gcatccgctc cgcctacaaa aaaggttttt
     4921 acgaatatat cgccgcatcg ggcgcggaca ttgtctgcgt gcaggaactc aaagcgcagg
     4981 aggctgattt gtctgccgat atgaaaaatc cgcacgggat gcacggccat tggcattgcg
     5041 ccgagaagcg cggttacagc ggcgtggcgg tgtacagcaa acgcaaaccc gacaatgtgc
     5101 aaatcggtat gggcattgaa gaattcgaca gggaagggcg gtttgtgcgt tgcgatttcg
     5161 acaggttgag cgttatttcg ctttacttgc ccagcggcag cagcgcggaa gaacgccagc
     5221 aggtgaaata ccgtttcctt gatgcgtttt accctatgct cgaagcgatg aaaaacgaag
     5281 gacgcgacat tgtcgtttgc ggcgactgga acatcgccca ccaaaacatc gacctgaaaa
     5341 actggaaagg caatcagaaa aattcaggtt tcctgccgga agaacgcgag tggataggca
     5401 aggttatcca caagctcggt tggacggata tgtggcgcac gctttatccc gatgtgccgg
     5461 gctacacgtg gtggagcaac cgcgggcagg cgtatgcgaa agatgtcggg tggcgcatcg
     5521 attatcagat ggttacgccc gaacttgccg ccaaagccgt gtccgcacac gtttataaag
     5581 atgaaaaatt ttccgaccat gcgccgctgg tcatggagta tgactatgct gccgaataaa
     5641 gttttgggta aatatgattg gaatgtggac ggaaaaacag gaataggcgc agcatgggtt
     5701 gtcgctgcgt ttatcctgcc catattggtg tgggcgatat ttatgttgtc gcggatgcaa
     5761 ggctggcttg cgccgaccaa ggcaaacccg acatgggcgt tggtgtggct gctgatctgc
     5821 atgccctgcc tgctgattgc gtccaaatgt ttgggttgga aaggctggcg gcgggtggtg
     5881 aatatttttg cctgcctgac cgtctgcgcc atattgagcg tacccgcttc gctgctcatc
     5941 gcctttaccc tccgggacct gctgaaatag gcggggcggt atgcagggtt ttgaactcga
     6001 agatttgacg ctttggctga tacgcgatgc cggagaggat cagatgtgga tagaccgttg
     6061 ggcgatcagt tatcccgtcg tgcagatgtc cgaagcgtcg gccggtcaaa gcatagggga
     6121 atggcaggca gggcttcgga cggcatttga acgcatacgt ggcaaatacg tcgccgttgt
     6181 cgcacacggt gcgggcgcgg ccgcattttt ggcgtggctg tatcaggcag acatcctgac
     6241 acggaagaaa attgccaaca tcatccttgt accgcagcgt cccgatattt ttcccgacga
     6301 tgcggaacac actttccaac gcgtccgctg tccctgccgt gccgcattgg tggtgtccga
     6361 acacggggat gtgccgcgag attgggcgca aaaacaggcg gatacgtgga acgcgcgcct
     6421 gttggtttcc ccgcattcgg gcagtttgaa cggtatgctc ggcggctggc agtggggtat
     6481 gaagctgatg caggaaatgt tgctggcgtg aacgcttgcc ttatcggaat ttcggctgta
     6541 gcaggaagtc cgcatatcgg gctgttttca ttgacgtgca ggcgcatcag gttcggataa
     6601 agtcgggctg ccgcataccg ccggaaatgg aatcctttat tttttacgtc ggcagtgccg
     6661 gcagggcgtt atccggcagg gttgcaagga aaatgccgtc tgaaacaggc ttcagacggc
     6721 atttccattc aaacagggct tacaggctgc ccaacacgat acgcaatacg cggcgcagcg
     6781 gctcggccgc gccccacaag agctggtcgc cgacggtgaa cgcgctgatg tattcgccgc
     6841 ccatgcccag tttgcggatg cgtccgacag ggacggacag cgtgccggta actttggcag
     6901 gagtcagctc gtggatgctg gcttcttttt cattggggat gactttcacc cagtcattcg
     6961 cgcctgccaa aatcgtttcg atttcggaaa caggcaggtc ttttttcaac ttcagagtga
     7021 tggcttggct gtggcagcgc atcgcgccga cgcggacgca caggccgtca atcacggttg
     7081 gattgtcgct gcggccgagg attttgttgg tttccacgcc gcctttccat tcttctttgg
     7141 actggccgtt gcccaaatcc acgtcaatcc acggaatcag gctgccggcg agcggtacgc
     7201 cgaagttggc tttcggatag tcttcgctgc gcaggaaatc ggatactttg cggtcgatgt
     7261 cgagaatcga gccggcagga tcggcaagtt cgtccgccac ttgggcgtga accgcgccca
     7321 taccgctgat gagttcgcgc atgtttttcg cacctgcgcc ggaagcggct tggtaggtca
     7381 tgctggttgc ccattcgacc aaatcgtttt ggaacaggcc gcccaaagcc atcagcatca
     7441 gggagacggt gcagttgccg ccgatgtagt ttttcacgcc gtttttgagg ccgttgtcga
     7501 taacgttgcg gttgacaggg tcgaggacga taatcgcgtc gtctttcatg cgcagagaag
     7561 atgccgcgtc aacccagtag ccgttccagc cgctgtcgcg cagggcttgg aagacggatt
     7621 tggtgtaatc gccgccttgg caggtaacga tgatgtccat tttggcgagt tcggcaacat
     7681 tgttggcatc taataatgtt ttagccgcct gaccgaaatc aggggctgcg ccgccgacgt
     7741 tggaagtggt aaagaaaaac gcttcaggaa tgtgggcgaa gtcgttttct tctttcatac
     7801 gctgcatcaa aaccgaaccg accataccgc gccagccgac gaaacctact ttcattgtgt
     7861 tgctccttga ggaaaaatcg t
//
LOCUS       DAWFBO010000091         7860 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFBO010000091 DAWFBO010000000
VERSION     DAWFBO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.091"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..1334)
                     /locus_tag="WGO87_001398"
     CDS             complement(<1..1334)
                     /locus_tag="WGO87_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HEZ0392752.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDATRLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
     gene            complement(1396..2745)
                     /locus_tag="WGO87_001399"
     CDS             complement(1396..2745)
                     /locus_tag="WGO87_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0392753.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRFLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKTNDRT"
     gene            2888..3514
                     /locus_tag="WGO87_001400"
     CDS             2888..3514
                     /locus_tag="WGO87_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0392754.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3578..4996)
                     /locus_tag="WGO87_001401"
     CDS             complement(3578..4996)
                     /locus_tag="WGO87_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0392755.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            5323..6051
                     /locus_tag="WGO87_001402"
     CDS             5323..6051
                     /locus_tag="WGO87_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0392756.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6110..6598)
                     /locus_tag="WGO87_001403"
     CDS             complement(6110..6598)
                     /locus_tag="WGO87_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0392757.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFGWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(7356..7820)
                     /locus_tag="WGO87_001404"
     CDS             complement(7356..7820)
                     /locus_tag="WGO87_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0392758.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPVASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2023 a         2127 c         1980 g         1730 t
ORIGIN      
        1 cccgacaggc ggatttgaag tttctccaca tatccggcgc gttcggcgcg gctggtttcg
       61 gggcggtgcg tattgagcga cgaccatttc caatgactgc gggctttgtt gagttcgggc
      121 gggagtctgg tggcgtccca cggggctttg cggctgtgca gctcgatatc cgactgtgcc
      181 gcgtgtccgc gcgtttgcag gacgtggagc aaatcgaggg cgcgggcggc gagcagggtc
      241 agggtttcag ggtcggtgtg caaggtttgg cggccggaga gtttgtcgga aatggtgcgg
      301 gtattgggca ggatgccgcc caaaaagccg ccgattgccg tacccaatcc gagcgagccg
      361 ccgagtgtgg cgatgtccag ccccaagccg atgagtgcgc cggttgccgc gcccgtgccg
      421 gtgcggatgc cgtattgttt gagcaattcg ctgtcgaacg ggtcttggcg gaaggcttgc
      481 ggcatccagt cgccgccgtc gatttcgctg tggtagaaac ggtagagggc aaacagccgc
      541 tgctgcatct gccgttcgag ttggcgtatt tccgcctgca tggtttgcag cacggtggcg
      601 gtatcctcgt tttcgtccac ttcctgcctg aaggcggcgg catcgagcag gaaatcggcg
      661 atttcgcggc gcgcttcgcc gtccagccgc tgccattcgc gccggcgcat ggctgtcagg
      721 cggtcaagtg tgctgcgttc gggcaacatg gtggcgaggt tttcccacag gcgtagttcg
      781 ccttcaaaat caaaggcgac ggtgtcgaac cctgcgaaaa cgtgcaggtt tctcctcgcc
      841 agcatggttg tccacgattc gggaggctgt ccgccggtaa agttgaacac gggcataacc
      901 ggtttggcgc accatgaaag gataatcaat tcatcccgat atttgtcgag aacgggttcg
      961 cgcgcgtcga tgacgtacat tgccatatcg ctttgcaaga cttgccgtaa gactttggct
     1021 tcctgattga aatcatggtg cgcgccgtgg ctgccgagaa actgttgcag ccgttcgatg
     1081 ccgtctgaac gattgtccgt atggttttcc agccattcca gcacgccgcc cgcgtcttcg
     1141 agtccgggcg tgtcgtacag gaaaaccagc gtgtctgcgc cgtcgctgat ggcggcttct
     1201 tcgacatgac gcgtggtcga tggggcgttt ctgacttcgc cgaaaccgct gtcgcgcaaa
     1261 agggtacgca ggagcgaggt tttgccggtg ttggtgtgtc cgacgacggc gagggaaagg
     1321 ggttgtttgt tcatgatgtt tttgaagaat ggattttcag acgacctttt aaggatggcg
     1381 gctttatggg gcgtttcaag tgcggtcgtt ggttttcaga cggccttcct gcgccgctct
     1441 gtcgggttcc agccacgccg cgccgcattc ggccagcgcg ttacgccaat gttccagctt
     1501 ttccgaaagg tcgtctgaaa gcccctgttc cgccaaaagc tgcaccaccg cgccgccctg
     1561 cgccgcttcc gaaagtcgga cgatctgccg caacacgccg cggtcgggca cagtttgggc
     1621 gcgcacgccg ataagcagtt gcgccggttt ctgcttcagc tctgtctcca gcgcggcaac
     1681 ctgttcccga ttggtggcaa cgcccttatc cagccattcc tgcgccagcc tgccctcgaa
     1741 ccattcgccg tcctgccatt cggtctccag catgaccgcc catttcggcg catcgttcaa
     1801 gacgattttc ggcgaaacgg cggacacggt ttcccgacgc gtatccgcat cggtgatttt
     1861 gttctgccaa cggcggatga ccgcctgata atagggtttt tccaaatcca atccgttttc
     1921 gcttgttttc aaaaggattt tgcataccgc ccaagccaag aagcgcggca ggatgccgta
     1981 gcaggcgata ctgccgacca gcagccccga ccaagcccgc gcatcggcaa tattgccgtt
     2041 cagacgacct tcgatgaccg cccgcgcatc aggcacggga aaacccagtt tcgcaggcag
     2101 ccatgccaac atttccacca gccgtaccga agacgaatcg cccaacagcg tgctttccca
     2161 gttgaacgta tattgccgca ccaaaagcag cagcaatacc gacaccagca ttccgagcag
     2221 cgtgcagagc cacaggctgt gcgacgttgc gcctattttc caacgtaccg aaggttgccg
     2281 ccactcgtcc gcatacagcc gcaacaccgc ctgatttaca gggtctttgc cccgaaacca
     2341 cgtcgccgga ctgctgaaaa aacgccccac tttcacgcgc aggaacaaca ttgccaacca
     2401 tactgccagc atcagcgtat tcatgcccaa cacgcccgcc aaaaccaaaa agaaattcag
     2461 accctgattg tccattagaa gataagttcc tgaaaaaccg gtaaaaaacg caaacgtcgc
     2521 cgccgccacc cacaaccaga acgaccccgc acgcacacgt tccaacgtct cccgcagcat
     2581 acggttcctg tcgatcatct ccgcccgacg gatgattttt tcctccgtac tgccgtccac
     2641 gcggcgcaaa gcctccgtcg cctgtacggg atcgccgctg aaaataaaac cgccttcgtc
     2701 caaaatacgg accagctcaa ccagttttcg ggatggattc aacataaaat gccgtctgaa
     2761 aataaaaaac agattttaac acacgcattt tcaagaatat tcacagtgta ggcaaagagt
     2821 aaatcccaca cagaggcaaa agtatcggcg taaactgact gcctctactt tcccgaaaga
     2881 ttgtgcgatg tatacaggcg aacgcttcaa tacttacagc catttgagcg gtttgattct
     2941 ggcggcggca ggtttggcgc tgatgctgct gaaaaccata ggacacgggg acggctaccg
     3001 tatcttcagc gtatcggttt acggcatcag ccttcttctg ctctatttga gttcctcgct
     3061 gtaccacgga attgcagccg gaaaactgaa aagcattttg aaaaaaaccg accactgcat
     3121 gatttatgtg ctgattgccg gaagctacac accgtttgca ctggtttctt tgagaaacgg
     3181 gccgggctgg acggtatttt cactgtcctg gctgctggcg gctgcaggaa tcgcacaaga
     3241 actcaccatt ggacggaaaa gcgaaaaacg actgctgtct attgcgattt atatcgtaat
     3301 gggctggatg gtcttggcgg taatgaaatc cctgacagcc tcactcccgc cggcaggact
     3361 ggcttggctg gcggcaggcg gtatgctgta cagcgtcggc atttactggt ttgtaaacga
     3421 tgaaaaaatc cgacacgggc acggaatctg gcatctgttc gtattgggcg gcagcatcac
     3481 ccaatttgtc agcgtgtacg gttacgtaat ctgaatgccg tctgaaaagc aaaacctccc
     3541 gatcctgaag attgggaggt tttctgtttg ccggacatca gcccttgtcg tggaactcgt
     3601 ggaattcata ctgataggac aaatcccgac ccgctttttt ctgtgccaaa taatcatcat
     3661 aaatggcacg gatttcctta cgcaacaaaa acagggctat caagttcggg ataaccataa
     3721 agccgttgaa catatcggac aggctccaaa ccaaatcgac tttgccgagc gtacctaaaa
     3781 cgatggcaag cagaaccaat gcgcgataga tgcccaagtg tcttcccctg aaaagaaaac
     3841 ggatgttgga ctcgccgaaa taataccagc cgataatggt cgtcaaggcg aaaaaggtca
     3901 gacacacggc aagcaattgc gaaccgaagc ccggaaatgc cttgttaaag gcaaattgag
     3961 taaccgccgc gccctgttcg cccgaaaggt tggcatcggt cagcaggata atcaatgccg
     4021 tagccgtaca taccaaaatc gtatcgataa acacaccgac aaatgccgcc ataccttgct
     4081 gcacaggatg cttcacatcc gcagtcgcgt gggcgtgcgg ggtagaaccc atccccgctt
     4141 cgttggaaaa cagaccgcgc gccacgccga aacgtatcgc ttcgcgcata ccgatacccg
     4201 ccgcaccgcc caaaacggct tcaggattga aggcggcggt aaagatgtgg ttgaacatcg
     4261 gcacaatata gtcggaaaat tcaaacagga taacgacggc gcacaaaata taaacaaccg
     4321 ccataaacgg cacgacaaac tgggcaatat tggcaatacg gttcacgccg ccaatcacaa
     4381 ccatgcccgc aaggacggca agcacaatac cgactgccaa agaaggcaca tcaaatgcaa
     4441 tggtaacggc agaagcaatg gagtttgcct gtgtcgcatt accgataaag cccaatgcga
     4501 taatcaacgc aatggagaaa aagcccgaca ggaaacgcgc cgcgcccctg ccgattttcg
     4561 gagtcagacc gtgcgtaata taaaacgccg gcccaccgat gtatttgccg tggctgacga
     4621 cgcggtattt ctgcgccagc agtgcctccg caaaaatcgt ggacatcccc aaaacggcag
     4681 aaacccacat ccaaaaaatc gcgcccggcc cgcctgcggt gatggcggtc gccacgccgg
     4741 cgacgttgcc cgtaccgatt tgcgcagata tggcaaccgc caacgcctga aactgcgata
     4801 aagacttgtc gtctttatcg cctttggcaa acaagccgcc gaatacagat ttgaatcccg
     4861 cacccagctt ggtaatctgc ggcgcaccga gatacagcgt aaaaaacagg ccgataccca
     4921 aaagcgcgta aatcagcagg tagtcccaaa ggaaccgatt gactgtaccc accagaacag
     4981 acaatatatt ttccataaaa taaaccttat cttacaatta aaatgactgc cttccaaaag
     5041 acattccaat aaggaaacac ggcgagcaga ccgtatttgc cgcaacagat gccttaaatt
     5101 gtcaacaatc ggggagaagc tgcgccatca taccgtaaaa tatcgttaat taaaacattt
     5161 ctttattttt aagcggaaag cggaggaaat cgctttcaga cggcatcgac aacggcacgg
     5221 cataaaacag gatattttgg gtacttgcaa cttatgttaa aatgccgacc gtaaaaaatc
     5281 tgacaaaaac agattaatta tttgaaataa gaaaggaaat ttatggcagg ccatagcaag
     5341 tgggcgaata tccagcataa aaaagcccgt caggatgcca aacgcggcaa aatcttcacc
     5401 cgtttaatca aagaaatcac cgttgcggcg cgtatgggcg gcggcgatcc cggtgcaaat
     5461 ccgcgcctgc gcctggcttt ggaaaaagca gccgaaaaca atatgcccaa agacaatgtg
     5521 caacgcgcca tcgacaaagg cacgggcaac ttggaaggcg tggaatacat cgaattgcgc
     5581 tacgaaggct acggcatcgg cggcgcggct ttgatggtgg actgcctgac cgacaacaaa
     5641 acccgcaccg ttgcggacgt acgccacgcg tttaccaaaa acggcggcaa cttgggtacc
     5701 gacggctgcg tggcgttcaa cttcgtgcat cagggctatt tggtattcga acccggcgtt
     5761 gacgaagacg cgctgatgga agcggctttg gaagccggtg cggaagacgt ggttaccaac
     5821 gacgacggtt ccatcgaagt cattaccgcg ccaaacgatt gggcgggcgt aaaatccgct
     5881 ttggaggcgg caggttacaa atccgttgac ggcgacgtta cgatgcgcgc ccaaaacgaa
     5941 accgaactct ccggcgacga tgccgtcaaa atgcaaaaac tgattgacgc gctggaagac
     6001 ttggacgacg tgcaagacgt ttacacttcc gccgtattga atttggactg atacgcagca
     6061 cagcagacat acaataaaat gccgtctgaa cctttcagac ggcattgatt tatttagccc
     6121 ttgcccacgc ccaccaaacc gtcaggacaa ccgccagaaa atacgccgcg ctccaaacag
     6181 gcaaagcaac ccccagcaga taatccggtt cggcacaatt tccgaacccg cgcacgacag
     6241 gctcgaacca accaaacaaa ggccagccct tcaatcggaa cgtccacggc gcaccgcacg
     6301 aaggagccgt acccggcggc agcgactgca accacaactg atatgccgca acagaaatac
     6361 ccgtaacggc cgggatgctg ataaagacag caccgaacaa accgcccgcc cttcttcttg
     6421 gtctgcacat caggacaatt gccgtacaca atgcggttgc caaaacgcat aaccgctgac
     6481 tgatacacaa aacgcaaggc tccataccca aaacatactg tgccgccaaa gaaccggcaa
     6541 atgcacagac cgaaacggca aacagcagcc aaacggcttt tctaaataac ggggtcattt
     6601 tctcaacaca ccaatcaaaa taccgatatg ccgattttgc tggatatatc ccgagatggc
     6661 aagggacgaa tcgacaccgg ctcaggtgcg gacgatttcg gttttcccga tatacacaag
     6721 acctaacaag ggacaaaacg gcgatttgcc cgcacaatgc ccgcaaaaaa atacatctag
     6781 aggatttgaa tttcggcaaa tttagtgaat caaaagttta tttcaatgaa atcatatgat
     6841 ttttttgaat aagcggattg acaggttttt gaaggaattt gttaccggat agccatcggg
     6901 caagtttttt gcaaaatttg aatcggtcgg gtaaattttc aaaaaataat tgacagcgga
     6961 taagaaacgg cggataattc ccgccgtcga gttgcttgat gcagcttgat ttttctcctc
     7021 tatttctcct ttgtagactt ggcacacatt caaccggatg tgtgcatttt tttatctccg
     7081 ctttttttga aatttaatta cttttattgt ttgaaattcc attctttaag aaatttaaca
     7141 agaaaaacgc ttgctttttg accggaaaag ctgcataatt cgttccgtta gctggttcga
     7201 gtagtcagtt aatagtttct cctctatttc tcctttgtag acttggcaca cattctattg
     7261 aatgtgtgca tttttttatc tgaagcaaca agcctctgtg cgtgatgttg ttatgtttca
     7321 tttaggtgtc aaaccgcata gccagcctga aatattcaat ccaaatccaa aaccggattt
     7381 tctttgacct cctccatcac aacataactc ctactctccg aagcgacagg cagttgcaat
     7441 aggatattgc ctagcatatc ccgataggca gacatatcgg gcaaacgtac tttaatcagg
     7501 tagtcgtatt cgcccgacac caagtggcat tccataattt gcggaatttt cagcacttct
     7561 tttttgaaat cttcgaaaat attgcccgat ttggattgca gcttcagctc gacaaaaacc
     7621 aatagcggtt tgcccaacag atggggattg aggtgggcgt gatagccgga aatataatgt
     7681 tcccgctcca aacggcgcac cctctctgta acgggcgtgg tggacaagcc taccttctcg
     7741 gcaagctccg tcatcgggat gcgggcattc tgttggagga tcttaagaat gcggaaatcg
     7801 attttatcta gttctttcat ttggattgcc ttgtatttat tattgatttt aacaaataga
//
LOCUS       DAWFBO010000092         7846 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFBO010000092 DAWFBO010000000
VERSION     DAWFBO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.092"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(90..1886)
                     /locus_tag="WGO87_001405"
     CDS             complement(90..1886)
                     /locus_tag="WGO87_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0392759.1"
                     /translation="MNTVSNYLSALREAMRAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVAGKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLIDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2344..3597
                     /locus_tag="WGO87_001406"
     CDS             2344..3597
                     /locus_tag="WGO87_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0392760.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3752..5263
                     /gene="lysS"
                     /locus_tag="WGO87_001407"
     CDS             3752..5263
                     /gene="lysS"
                     /locus_tag="WGO87_001407"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0392761.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNQGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRAVNGTANITYNGKEVDLESPFERLTILDAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDSKPGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5340..5528)
                     /locus_tag="WGO87_001408"
     CDS             complement(5340..5528)
                     /locus_tag="WGO87_001408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0392762.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(5532..6409)
                     /locus_tag="WGO87_001409"
                     /pseudo
     CDS             complement(5532..6409)
                     /locus_tag="WGO87_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            complement(6439..7566)
                     /locus_tag="WGO87_001410"
     CDS             complement(6439..7566)
                     /locus_tag="WGO87_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0392763.1"
                     /translation="MSNWKPNIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
BASE COUNT         1700 a         1993 c         2208 g         1945 t
ORIGIN      
        1 cttgcggcaa gatgaaaacg gtgcgggatg ttttgggaaa ccaatgccgt ctgaagggct
       61 ttcagacggc attttttgcg ccgtgctgtt taacgcgcca gcggttcggt gcgtttgatc
      121 agccacgctt ttgccgcgcc ttcggtcagc ggctcgaggc ggcggcggac ttcggcgtgg
      181 tagcggttga cccagtcgat ttcgccgtcg gtcatgaggg cggtgtctat caggcgggtg
      241 tcgatggggc agagggtcag ggtttcaaaa cagaggaagc tgccgaattc ggtttcttga
      301 ggggcggcga cggcttggtt ggcggcaagg ttttcaatgc ggatgcccca ttttcccggg
      361 cggtagagtc cgggttcgat ggaggtaacc atgccttttt tcatggcggt ttcgggcgtg
      421 gcgggggcgg cgaaggcgat gcgctgcggg ccttcgtgga cgttgaggaa atagcctacg
      481 ccgtgtccgg tgccgtggcc gtagtcgcat tgcgcctgcc acaggggttt gcggcaaatc
      541 gcatcaatca gcggcgaggg gatgttttct gggaacacgg cttcggcaag cgcgatatgg
      601 gctttgagaa cgagggtgtt gtcgcgtttt tgttcggcgg tgggtgtgcc gacagggacg
      661 acgcgggtga tgtcggtcgt gccgcctttg tattgcgcgc cggagtcgat gagcagcagc
      721 ccgttgccgc tgatggtgct gtggctttcg ggcgttgcgc tgtaatgcgg cagggcgccg
      781 ttggcgttga agcctgcgat ggtgtcgaaa ctcaatgaca caaaacccgg gcgcacgctg
      841 cggtggcggt aaagcatggt gtccacgtcg atttcggtca ggctgccgcc gttgccgatg
      901 atgtcttcaa actcggcgaa gaaaccgcac aacgccgcgc cgtcgtgttc catcgcttcg
      961 cggatgcggg cgatgtcggc ttcggatttg caggatttga acagcgtgga cgggttgatt
     1021 ccctcgataa ggcgcacgct ttcgggcagg cgcacaagcg tgctgacggc ggttttgttc
     1081 ggttcgataa gcaacgcgcc gccgatttgc gcgagtttgc cggcaacttg ggcgtaaggc
     1141 tcgaccgtga tgcctgcggt ttgcagcgcg gcggcggctt cggcgttcag acggcatcgg
     1201 tcggtaaaca ggacggcgtt gtctttgcca atcagcagga aggacacgaa aacgggattg
     1261 aaaggcacgt cgctgccgcg caggttggtc agccaggcga tgtcgtcaag cgaggaaacc
     1321 aagtggtaat ccgcgccttt ttccgccatc acggcgcgca cgcgggcgag tttttcggcg
     1381 gcggtttcgg agacgtaggc ggggtcgtgg atgaacacgg tttcggcagg aatggccggg
     1441 cggcttgtcc acacttggtc aagcagatcg tttgggtgtt cgatgcggat atttttggcg
     1501 gcgagtgatt gcgccaaagt gcgtttgccg gtgagcgaga ccatatcgga agggatgccg
     1561 acggcggcgt tttcgggcag gtttgccgcg agccattcgt tgtacggcgg cacttgaccg
     1621 cttttttgca gctcgatgcc gctgccggaa agctgtttgg cggcttgttc ccaatagcgg
     1681 ctgtccaccc atacacccgc ttcatcggtg gtcaggacaa atgtgccgac cgagccggta
     1741 aagcccgaca attcgcggcg cgcctgccag tgtgcgggca ggtattcgga caggtggggg
     1801 tcggcggaag ggatgacgag tgcgtccaag ccttgcgccc tcatggcttc gcgcaatgcg
     1861 gacaggtaat tcgatacggt attcataaca gttcctccaa tcgggttttg cggcttcaga
     1921 cggcatggac ggaaatctga aatgccgtct gaaaaacata accatcttat caaatcgcca
     1981 gcctgctgca aaacggacgg gctgatcgga tggtgcgtgt ttgcgcgaaa aaagcgatgt
     2041 aaggttgttt gttgtaaaat tttaggcata aaatgtcgtt tcatgccttt ttttggaagt
     2101 aatatggaaa atatagtcaa gtttgagcat gaagttctaa tagtgtgtgt tggcaggtat
     2161 ttggattacg ttacaatacg cgctttcatc aaaatttccc taccgagcgg tatcctgttt
     2221 ttgaagacgg taggatttgg gtttgtttgg gggcaatccc gcccaaattg gagcaataga
     2281 tagggctgtg tccgaatatg cggctctgtg tgttgaaaca cggttaaaaa tacggaagac
     2341 tttatgtccg aacaacatat ttcgacttgg aaaagtaaaa tcaacgcatt gggaccgggg
     2401 attatgatgg cttcggcggc ggtcggcggt tcgcacctga ttgcctcgac gcaggcgggc
     2461 gcgctttacg gctggcagat cgcgctcatc atcatcctga ccaacctctt caaatacccg
     2521 tttttccgct tcagcgcgca ttacacgctg gacacgggca agagcctgat tgaaggttat
     2581 gccgagaaaa gccgcgttta tttgtgggta ttcctgattt tgtgcatcct ctccgccacg
     2641 attaacgcgg gcgcggtcgc cattgtaacc gccgccatcg tcaaaatggc gattccctcg
     2701 ctgatgtttg atgccggcac ggttgccgcc ttgattatgg catcctgcct gattattttg
     2761 gtgagcggac gttaccgcgc tttggatcgc gtttccaaaa tcatcatcgt tactttgagt
     2821 atcgccacgc ttgccgccgc cggcatcgct atgtcgcgcg gtatgcagat gcagcccgat
     2881 tttatcgagc cgacaccgtg gacgcttgcc ggtttgggct tcctgatcgc gctgatgggc
     2941 tggatgcccg cgccgattga aatttccgcc atcaattctt tgtgggtaac cgaaaaacaa
     3001 cgcatcaatc cttccgaata ccgcgacggg atttttgatt tcaacgtcgg ttatatcgcc
     3061 agtgcggttt tggctttggt tttccttgca ctgggtgcgt ttgtgcaata cggcaacggc
     3121 gaagcagtac agatggcggg cggcaaatat atcgggcaat tgatcaatat gtacgccgtt
     3181 accatcggcg gctggtcgcg cccgctggtg gcgtttatcg cgtttgcctg tatgtacggc
     3241 acgacgatta ccgtcgtgga cggctatgcc cgtgccattg ccgaacccgt gcgcctgctg
     3301 cgcggaaaag acaaaacggg caacgccgaa ttctttgcct ggaatatttg ggtggcgggc
     3361 agcggtttgg cggtgatttt ttggtttgac ggcgtaatgg cgaatctgct caaatttgcg
     3421 atgattgccg cttttgtgtc cgctcctgtg tttgcctggc tgaattaccg tttggtcaaa
     3481 ggtgatgaaa aacacaaact cacatcaggt atgaatgccc ttgcattggc aggcttgatt
     3541 tatctgaccg gttttaccgt tttgttctta ttgaatttgg cgggaatgtt caaatgataa
     3601 cgatgccgtc tgaaaccgca aaccgctttc agacggcatt gccgcgttta tggaaaaaaa
     3661 tgccgacatc gcgtaaaata cgcgcaaatt ttgtgattcg gtcaggtcgt ctgaaacaga
     3721 ttgcgcctga tttatttttt cggaaaaccc tatgagcgaa caaaaccatc cgcaaaccga
     3781 gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactgc acaacatccg
     3841 ccaacagcgc aacgcctatc ccaacgactt caaacgcgac agcttcgccg ccgatttgca
     3901 cgcccaatac ggcgaaatcg gcaaagaaga actcgatccg caaggcattc ccgtcaaagt
     3961 ggccggccgc atgatgctga agcgtcaaat gggcaaggcg agctttgcca ctattcaaga
     4021 cgtgtccggg caaatccagc tttatctgaa caacaaaggc gtgagccaag aagttttgga
     4081 cgacttcaac cattgggatt tgggcgacat cgtcggcgcg gaaggcactt tgttcaaaac
     4141 caaccagggc gaactgaccg tacgcgtgtc cggcatccgc ctgctgtcca aatccctgcg
     4201 ccctctgccg gacaaacata aaggcttgag cgatcaggaa accaaatacc gccaacgcta
     4261 tgtcgatttg attgccaacg aagaatcgcg caataccttt atcaaacgca gccaaatcat
     4321 ccaatccgtg cgtaatttta tggtgggcga gcattatctc gaagtcgaaa ccccgatgat
     4381 gcacccgatt cccggcggcg cgacggcaaa acccttcgtt acccaccaca atgccttgga
     4441 cattccgctc tacctgcgca tcgcccccga gctgtatctg aaacgcttgg ttgtcggcgg
     4501 tttggaacgc gtgtttgaaa tcaaccgcag cttccgcaac gaaggtatgt ctgtgcgcca
     4561 taaccctgaa ttcaccatga tcgaattcta cgaagccttc tccgactacg aacgcatgat
     4621 gcaaatggcg gaagacatca tccgcaacgc ttcccgcgcg gtaaacggca cggcaaacat
     4681 cacttacaac ggcaaagaag tcgatttgga aagcccgttc gagcgtctga ccattctcga
     4741 tgccatcaaa aaatacaatc cgcactacac cgacgagcag ttgaacgatg cggagtggct
     4801 gaaaaaagaa atcgtcaaac acggcgaaag cctgcctccg tctccgggca tcggcagcct
     4861 gcaacttgcc ctgtttgaag gttgcgccga gggcaaattg tggaacccga ctttcattat
     4921 cgactaccct gtcgaagtgt ccccgttggc acgcgcatcc gacagcaaac cgggcttgac
     4981 cgaacgcttc gagcttttcg ttgtcggccg cgaactggca aacggctact ccgagttgaa
     5041 cgatccggaa gaccaagccg aacgcttcaa ggcacaagtg gcgcaaaaag acgcgggcga
     5101 cgacgaagcg atgcactacg atgccgacta catccgcgcg atggaattcg gcctgccgcc
     5161 gaccggcggc tgcggcatcg gcatcgaccg cttggtcatg ttgctgaccg attcgcaaac
     5221 catccgcgac gtgattctgt ttccgcagat gcgccccgag tagtcataaa aacagttgaa
     5281 atggcaatgc cgtctgaacc cgattggatt cagacggcat tttgtatggc ggtacggatt
     5341 tattcgcttt ctaactgacc gacccattcg gacaaggcag tcaggcgttg ttcggatttc
     5401 gccacgaacg ggttttcggt atcccacgcg tagcctgcca aaatcgaaga aagcatacgg
     5461 ctgatttccg ggctgcggtc gggcgcgtac acgacgtttt ggaagcggaa atcgtaccaa
     5521 ccgtccacac atcaggggtg gtaatccgaa tcaacacgcc ttcttgcctg tgcggcggac
     5581 ataaaccgtt ttttgactcc agcaccacac ctaaaatcgt gctgccttcg ggcaggtcaa
     5641 aggcttcaga cggcctgccc gtctgataaa tcagcatcct gcccgcaata tcgataacaa
     5701 ggtgcgcgac gaccagccgc ttgtcggaca gttcaatcag cacgatgtcc tcgtccgcag
     5761 ggcgcatacg cggctcgacc accaccaaat cccctttttc gataaccggc gacatggacg
     5821 tgtcgggcat ggcaacggca aacgtgtact gcgagggggc gaccaccgaa gcaaacaagt
     5881 cgaaactgcc tgtgagtttg cctgtacggg cgaaagccgc cgcctgacgg tctgacaaca
     5941 gggggcgtgg tgtgagcctc atcgacagtg ctttgccgtc ggcatcgctg tggttgtgcg
     6001 tatgcgtatg atggttgttg atggtcgtat cgccttggac atcgttaagg actatgctgt
     6061 ttgaacctgc cacggatttg gtcatctcat tgtaggcttg cgtcgtgatg cgctcttgct
     6121 tggtcatacc tgttacagcc tgtttcagca tttctaacgc tatgtgattc ttcctaaggg
     6181 tcgctatacc ggcttttgca gcttgctcat taccacaaga ggggcaaccc cagctacttc
     6241 ctatgaacat attggagtac ctcgaacagg tttggtttcc atgtttaggg cagttgatta
     6301 cacacggctt ggctgtaccc tcaaactcga ccaacttaat gccacgatga ccgaaacgtt
     6361 cctcaacctc agcagcagct tcttcaaatg tttttgacct tcttgccata ttgacctccg
     6421 gattattgat tattttggct atgctttaac gagggattgg aatgaggtaa agctgttgcc
     6481 ctctccccgc aatagttcca ttaggcgcgg atgaatgaat agtttgtccc tgccgatggc
     6541 gatttcttgc agcacaccta tgtctgaaag ctctttcagg tacttagagg ccgtctgccg
     6601 tttggctatc cctgccgctt ctaggttggc aatgcgtgta tatggctgct caaacagaag
     6661 atttaccagt tcgtgcgtgt agattccttg tgcgtgtgtc cgtatgtgtt gacgtgtctg
     6721 ctcaaacagg cggcgtatcg catctatttt cgctaccgtc caatcggcgg tgtcggctac
     6781 gccgtctaag atgtagatta tccagctttc ccagtcctgc cgttcggtta cgcctaaaag
     6841 caggcggtaa tagtccgccc tgttttcgat aatatagcgg ctcaaataca aaataggcaa
     6901 atccaaaagc cctttttcaa tcaatagcag gctgttcaat atgcgccccg tccgcccgtt
     6961 gccgtccgta aacggatgga tggcttcaaa ttggtaatgt gccgccgcca tgatgataag
     7021 cgggtctaaa tcgccgcttt cgtgaataaa ccgctcccaa tttgccagct tgccgcgtat
     7081 ggtttcttct ccttcgggcg gggtatagac aacatttccg ctgttgcctc cttttagggc
     7141 tgtgccgcct gttttgcgga tggccatttc gtaggggtgc ttgatggcgt tgcagaccat
     7201 gatggcggtt tgtgtgcata aagggcggct cgtcagtgat tcatagcctg caaacagggc
     7261 ggtgcggtat tgcagggctt ctttcgtggc agggtcttgc cgttccgtat ccatttgcag
     7321 ggattgaaac agcttgtccg tggtggttac gatgttttca atttccgaac ttgcacgggc
     7381 ttccataaca ggaagggtgt taatcagcat ggcttgattc ggtatcaatt ctgccgcctg
     7441 ctttaaacgg gcaagggatg cacgggcggc tatacaacgt ttcaggatgg ttttgctttc
     7501 aatatcctgt tttggcggca ggggtggtaa atcgttatag ggaatattgg gtttccagtt
     7561 gctcatattt aaaatttcgg aaaatttaaa gatgtttcca gtatatgttt acgccgtgta
     7621 tatatcaagg atatatgttt aaaaatttgg cttttgtaaa tatatgggcg gtaaaaccgc
     7681 attattttat gttgttacct attttacacc ttgtctgcac acgatttcca aacaacctaa
     7741 aaagaaacct gccgaaaggc aggtttaaag aggtcatttt aaatgtgaac taaagcttac
     7801 accctcgtag ggtatcgtgt atatcagacg tacttaatat acacta
//
LOCUS       DAWFBO010000093         7643 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFBO010000093 DAWFBO010000000
VERSION     DAWFBO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.093"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(9..425)
                     /gene="dksA"
                     /locus_tag="WGO87_001411"
     CDS             complement(9..425)
                     /gene="dksA"
                     /locus_tag="WGO87_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0392764.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(572..1363)
                     /locus_tag="WGO87_001412"
     CDS             complement(572..1363)
                     /locus_tag="WGO87_001412"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0392765.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1412..1819)
                     /locus_tag="WGO87_001413"
     CDS             complement(1412..1819)
                     /locus_tag="WGO87_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0392766.1"
                     /translation="MGMRTIKYLAMALLLMFTVAGCQLAGWYPCPSLSGWCMPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSECVRW"
     gene            complement(1801..2505)
                     /locus_tag="WGO87_001414"
     CDS             complement(1801..2505)
                     /locus_tag="WGO87_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0392767.1"
                     /translation="MTVLQQRYREVSDRIGKLVLQAGREPHSVRLIAVSKTFPSDGIR
                     KVYAAGQRDFGENYIQEWYGKTEELADLPDIVWHVIGDVQSNKTKFVAERAHWVHTIC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVVLAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNTAGVKADVLSMGMSDDMPAAIACGATHVR
                     IGSAIFGKRSRLECVQ"
     gene            2624..3667
                     /locus_tag="WGO87_001415"
     CDS             2624..3667
                     /locus_tag="WGO87_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0392768.1"
                     /translation="MQITDLLAFGVKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPRIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTRDGNG
                     RVASHEILISNPAVRNLIRENKIAQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARKRAQNSESMSF"
     gene            3831..5057
                     /locus_tag="WGO87_001416"
     CDS             3831..5057
                     /locus_tag="WGO87_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0392769.1"
                     /translation="MSTNNLHDILDEMVQVYSQKKQSRSETPAEIGAHLHPLLDRLCE
                     TAEAQSASDILISKGFPPSLKINGALTPQPQKALTGEETAAITASTMNAEQSEIFQRD
                     GEINYSVQSRSGTRYRANAYHSQGHAGLVLRRINHIIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRENMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHKQILIDIALNLSGIVCQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNINKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5343..7493
                     /gene="yccS"
                     /locus_tag="WGO87_001417"
     CDS             5343..7493
                     /gene="yccS"
                     /locus_tag="WGO87_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0392770.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1895 a         2535 c         1655 g         1558 t
ORIGIN      
        1 tgccgccatc agtcggcaaa ctgttttttc atcctctctc ggcgttcttg ggactcaaca
       61 gataaagtgg ctgtcgggcg tgccagcagc cgcttcagac cgataggctc tcccgtatcg
      121 gcacagaatc cataatcccc ttcatcaata ttgcggatgg tcgcctgtat tttactgaga
      181 agttttcgtt cccgatcgcg ggtacggagt tccaatgcgt actcttcttc ctgtgtggca
      241 cggtcggcag gatcgggggc tgattcgtgt tcttggagat gccctgtcgt agcggaagca
      301 ttttcgatga gttcgtcttg catttttacc agcaattcgc ggaaaaaagc caaatgctcg
      361 tcattcatat aatcgtcttc cggcccgttc caattcaaaa tatcttgttc tgtcagcttt
      421 gccataatgt atatcctctc ggttcaccga aaaaaacaaa gcaccacaaa gtggctttat
      481 tcctttgatt tttataaggc ttattgccta gggcgttttt gaaaacgcgc atcataacac
      541 gttttggatt ggttttttta ttttctttac attattgata ttgccgttcc atttcctgcg
      601 aacggcgcac acaggcacaa acgccctcgc ttatggcttc ggcgacacga tgccgcctga
      661 aagcttccac ggcttcgtgg gttgtcccgc cttttgacgt tacatttttt tgaagcttct
      721 cgaaatcttc acccgtctgc tcggcaaggg caaccgctcc tttaaacgtt gccagactga
      781 gcgcgcgtgc ttctgccata tcaaaccctt gtcggatggc agcattttgc aatgcgtcca
      841 gcagataaaa cacataagcc ggtccgctgc cgctgatacc ggtaatgctg tgcatttgcg
      901 cttcatcttc caaccaaacg gtcaaaccga ctgatttcat gattcgatcg gcaatcctgc
      961 ggtctgtttc cgatacttcc gcttcggcaa acataccgga tacgcccagc ccgatttttc
     1021 cgggtgtatt cggcataacc cggacaatgc ggcgtgttcc cccgaggtaa cggctgagcg
     1081 tatcaaccga caatccagct gcaacagaaa gcaccaatgc gccgttggtg cggatatttt
     1141 tgcacgcggc ttccatatcc tgcggcttga cggcaaggat taaaacatcg tcggaatgaa
     1201 gctccggcaa ggtttcagaa gtttcgaccc ccaactcttt ttgcaaacgt tcgcgctttt
     1261 ccgcaccccg gttggcaata tggatgcggt aaccgccttg tttgaccaat ccgcccgcaa
     1321 cggcagccgc catattgccg ccgccgagaa aataaacatt cattatttct accctattca
     1381 aaattacctt actaaattca ttcccaattt tttaccatcg gacacactcg gacagaccgt
     1441 tgcgccgcaa cccctgcttt ttcaaacaat caaaaccttc ctgtttgaac gtctcaatca
     1501 aaggtttgcc gtcacgcgta cgccaatcca acccgcaggc ttcaaacttc cctattttcc
     1561 tgtaaaagta agattgttgt gcggattcat attcgtttgc cctaacggaa cgattgccgt
     1621 ctgaaagcgg tatctcgtag tcctctaaag acggcggact ctcgccgcca atatcccaaa
     1681 aatcgatggc agccggtttt ctcggcatac accagccgga caacgacgga catggatacc
     1741 aacccgccaa ctgacagccg gcaaccgtaa acataagcaa taacgccatt gcaagatatt
     1801 ttattgtacg cattccaatc tgctcctttt cccgaaaatc gcactgccga tacggacatg
     1861 tgttgcaccg cacgcaatgg cggcaggcat atcgtccgac atccccatag acagcacatc
     1921 cgccttaacg ccagccgtat tgaggtcggc aagcagtttc cgcatcgttt gaaattgcac
     1981 cttcaactcc gtttcactgc tgttggcttt ggcaacacac atcagtccgc gtacgacgat
     2041 attcggcaac ttcgccactt cgaccgcaag cacgaccgct tcttcgggcg cgacaccgtg
     2101 tttcgccgcc tcgcccgcaa tgttcacctc gatacacacc tgcaaaggcg gcattgagga
     2161 aggacgttgc ccgctcagcc ggacagcggt tttcagacgg catatggtat gcacccaatg
     2221 cgcgcgttcg gcgacaaact tggttttgtt ggactgcaca tcgccgatga cgtgccacac
     2281 gatgtcgggc aaatccgcca actcttccgt tttaccgtac cactcctgaa tatagttctc
     2341 gccgaaatca cgctgtccgg cagcataaac tttgcggatg ccgtctgaag ggaaagtctt
     2401 actgacggca atcagtctga cagaatgcgg ctcccttccc gcctgcagaa ccaattttcc
     2461 gatacggtcg gacacctccc gataacgctg ttgcaacacc gtcatagatt atcccctatt
     2521 aaaaatgatt aaacagttga aacccctcca gtcagggcgg tacaatcaag gttgttagaa
     2581 tcattccaac caatcgaaac attatactaa acagagccgc attatgcaga ttaccgactt
     2641 actcgccttc ggcgttaaaa acaaagcatc cgaccttcac ctgagttcgg gcatatcccc
     2701 tatgattcgg gttcacggcg atatgcggcg catcaacctt cccgaaatga gcgcggaaga
     2761 ggtcggcaat atggttacct ccgttatgaa cgaccaccag cggaaaatct accagcaaaa
     2821 cttggaagtc gacttctcgt tcgaactgcc caatgtcgcc cgattccgcg tcaacgcctt
     2881 caacaccggc cgcggccccg ccgccgtatt ccgcaccatt cccagcaccg tcttatcgct
     2941 ggaagaattg aaagccccgc gcattttcca aaaaatcgca gaatcgccgc gcggtatggt
     3001 attggttacc ggccccaccg gttcgggcaa atccaccacg cttgccgcga tgatcaacta
     3061 cattaacgaa acccagccgg cacacatcct gaccatcgaa gacccgatcg aattcgtcca
     3121 ccaaagcaaa aaatccctga ttaaccaacg cgagctgcac cagcacactc tcagcttcgc
     3181 caacgcgctg agttccgcat tgcgcgaaga ccccgacgtt atccttgtcg gcgagatgcg
     3241 cgaccctgaa accatcggct tggcactgac cgccgctgaa accggacact tggttttcgg
     3301 cacgctgcac acgaccggcg cggcaaaaac cgtcgaccgt atcgtggacg tattcccggc
     3361 gggggaaaag gaaatggtgc gctctatgct gtccgaatca ctgaccgccg tcatttccca
     3421 aaacttgctg aaaacgcgcg acggcaatgg ccgtgtcgcc tcgcacgaaa tcctgatttc
     3481 caaccccgcc gtccgcaacc tcatccgcga aaacaaaatc gcgcagatta actccgtcct
     3541 gcaaaccggg caggcgagcg gtatgcagac gatggaccaa tcgctgcaat cactggtgcg
     3601 ccaagggctg attgcaccgg aagtcgcacg caaacgcgcg caaaacagcg aaagtatgag
     3661 tttctgatac acaaccgcct tccggccata ccggcgggaa aacaaggtgc aaacacgccg
     3721 ggcgggacgc gacatccagc ccagcatacc cttccgaata ggaagcgtcc gccttcctgt
     3781 tgaaacctgc cgccgcaaac tgcaaggctt aaactgaaaa gaagttaacg atgagtacca
     3841 ataacctgca cgacatcttg gacgaaatgg ttcaagtgta ttctcaaaaa aaacaaagcc
     3901 gatccgaaac cccggccgaa atcggcgcgc acctccaccc gctgctcgac cgcctgtgcg
     3961 aaaccgcaga agcgcaaagc gcgtccgaca tccttatcag caaaggattc ccgccctcgt
     4021 tgaaaatcaa cggcgcatta accccgcagc cgcaaaaggc tctgacgggc gaggaaaccg
     4081 ccgccatcac cgcatcgacg atgaacgccg aacaatcgga aatattccag cgcgacggcg
     4141 aaatcaacta ctccgtccaa tcgcgcagcg gcacgcgcta ccgcgccaac gcctaccaca
     4201 gccaaggcca cgcaggtttg gttttgcggc gcatcaacca catcatcccg caaatgcagg
     4261 aattgggcct gcccgaaaaa ctcaaagacc tcgccgtcgc gccgcgcggg ctgttgatta
     4321 tcgtcggacc taccggttcg ggcaaatcca ccacgatggc gactatgctc gaacatcgca
     4381 acaaaaccct gcccggccat atcgttacca tcgaagaccc gatagagttt atctacaaac
     4441 cgcgccgctg catctttacc cagcgcgaaa tcggcgtcga caccataaac tggcagacgg
     4501 cggtacaaaa cgctatgcgc cagtcccccg acgtggtctg catcggcgaa gtccgcagca
     4561 gggaaaatat ggaatacgcg atgcagctcg cccaaaccgg ccacctgtgc atttttacgc
     4621 tccacgccaa caccgcgccg cagtcgctcg aacgcatact caacttctac cccaaagaac
     4681 agcacaaaca aatactgatc gacatcgccc tcaacctgtc cggtatcgtc tgccaacgcc
     4741 tcgccctcaa aaaagacaaa acgggcagga cggcggttgt cgatttgctc atcaacacgc
     4801 ccgccatcca agacttcatc ctgaagggcg acctgatgaa catcaataaa atcatggaaa
     4861 ccgccaaaac cgacggaatg cagacgatgg accaaaacct tttcgagctg taccgtcacg
     4921 gcatcatcag ttacgaagaa gccctgcgcc agtccgtttc cgccaacaac ctgcgattgc
     4981 acatccaact gcacaaagaa ggcaaaacgc ccgaactcct ttacgacagg gtcaacggtc
     5041 tcaacctcat ttcctgatcc gcaaaaccca atgccgtctg aaaaccgcat ccccgttttc
     5101 agacggcatg attttatccg ccccattcat gtgctacact ttatagtgga ttaaatttat
     5161 agtggattaa caaaaatcag gacaagacga cgaagccgca gacagcacaa atagtacgga
     5221 accgattcac ttggtgcttg agcaccttag agaatcgttc tctttcagct aaggcgaggc
     5281 aacgccgtac tggtttttgt taatccacta tattcacttc ataacaataa accggtaaaa
     5341 ccatgaaaac cccactcctc aagcctctgc tcattacctc gcttcccgtt ttcgccagtg
     5401 tttttaccgc cgcctccatc gtctggcagc taggcgaacc caagctcgcc atgcccttcg
     5461 tactcggcat catcgccggc ggccttgtcg atttggacaa ccgcctgacc ggacggctga
     5521 aaaacatcat cgccaccgtc gccctgttta ccctctcctc gctcacggca caaagtaccc
     5581 tcggcacagg gctgcccttc atcctcgcca tgaccctgat gaccttcggc tttaccattt
     5641 taggcgcggt cgggctgaaa taccgcacct tcgccttcgg cgcactcgcc gtcgccacct
     5701 acaccacact tacctacacc cccgaaacct actggctgac caaccccttc atgattttat
     5761 gcggcaccgt actgtacagc accgccatca tcctgttcca aatcatcctg ccccaccgcc
     5821 ccgtccaaga aagcgtcgcc aacgcctacg acgcactcgg cggctacctc gaagccaaag
     5881 ccgacttctt cgaccccgat gaggcagcct ggataggcaa ccgccacatc gacctcgcca
     5941 tgagcaacac cggcgtcatc accgccttca accaatgccg ttccgccctg ttttaccgcc
     6001 ttcgcggcaa acaccgccac ccgcgcaccg ccaaaatgct gcgctactac ttcgccgccc
     6061 aagacataca cgaacgcatc agctccgccc acgtcgacta ccaagagatg tccgaaaaat
     6121 tcaaaaacac cgacatcatc ttccgcatcc accgcctgct cgaaatgcag ggacaagcct
     6181 gccgcaacac cgcccaagcc ctgcgcgcaa gcaaagacta cgtttacagc aaacgcctcg
     6241 gccgcgccat cgaaggctgc cgccaatcgc tgcgcctcct ttcagacagc aacgacaatc
     6301 ccgacatccg ccacctgcgc cgccttctcg acaacctcgg cagcgtcgac cagcagttcc
     6361 gccaactcca gcacaacggc ctgcaggcag aaaacgaccg catgggcgac acccgcatcg
     6421 ccgccctcga aaccggcagc ctcaaaaaca cctggcaggc aatccgtccg cagctaaacc
     6481 tcgaatcagg cgtattccgc catgccgtcc gcctgtccct tgtcgttgcc gccgcctgca
     6541 ccatcgtcga agccctcaac ctcaacctcg gctactggat actactgacc gcccttttcg
     6601 tctgccaacc caactacacc gccaccaaaa gccgcgtccg ccagcgcatc gccggcaccg
     6661 tactcggcgt aatcgtcggc tcgctcgtcc cctactttac cccctccgtc gaaaccaaac
     6721 tctggatcgt catcgccagt accaccctct ttttcatgac ccgcacctac aaatacagct
     6781 tctcgacatt tttcatcacc attcaagccc tgaccagcct ctccctcgca gggttggacg
     6841 tatacgccgc catgcccgta cgcatcatcg acaccattat cggcgcatcc cttgcctggg
     6901 cggcagtcag ctacctgtgg ccagactgga aatacctcac gctcgaacgc accgccgccc
     6961 ttgccgtatg cagcaacggc gcctatctcg aaaaaatcac cgaacgcctc aaaagcggcg
     7021 aaaccggcga cgacgtcgaa taccgcgcca cccgccgccg cgcccacgaa cacaccgccg
     7081 ccctcagcag caccctttcc gacatgagca gcgaacccgc aaaattcgcc gacagcctgc
     7141 aacccggctt taccctgctc aaaaccggct acgccctgac cggctacatc tccgccctcg
     7201 gcgcataccg cagcgaaatg cacgaagaat gcagccccga ctttaccgca cagttccacc
     7261 tcgccgccga acacaccgcc cacatcttcc aacacctgcc cgaaaccgaa cccgacgact
     7321 ttcagacagc actggataca ctgcgcggcg aactcgacac cctccgcacc cacagcagcg
     7381 gaacacaaag ccacatcctc ctccaacagc tccaactcat cgcccggcag ctcgaaccct
     7441 actaccgcgc ctaccgccaa attccgcaca ggcagcccca aaatgcagcc tgaaaaagtt
     7501 tcggcatttt gtaagagagg gcagattgtc agacaggttg caagatagtg gataaggctt
     7561 tgcgcagggt aaatgcgtag caactgaacc gtcattccca cgaacctaca tcccgtcatt
     7621 cccacgaaaa cagaaaacca aaa
//
LOCUS       DAWFBO010000094         7597 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFBO010000094 DAWFBO010000000
VERSION     DAWFBO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.094"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="WGO87_001418"
     CDS             complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="WGO87_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0392771.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RTATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1555..1710
                     /locus_tag="WGO87_001419"
     CDS             1555..1710
                     /locus_tag="WGO87_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392772.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1707..2138
                     /locus_tag="WGO87_001420"
     CDS             1707..2138
                     /locus_tag="WGO87_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0392773.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2272..6621
                     /gene="app"
                     /locus_tag="WGO87_001421"
     CDS             2272..6621
                     /gene="app"
                     /locus_tag="WGO87_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0392774.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYKPDNSHPYNGDYHM
                     PRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGHHYWRYDDDKHGDLSYSGA
                     WLIGGNTHMQGWGNNGVVSLSGDVRHANDYGPMPIAGAAGDSGSPMFIYDKTNNKWLL
                     NGVLQTGYPYSGRENGFQLIRKDWFYDDIYRGDTHTVFFEPRSNGHFSFTSNNNGTGT
                     VTETNEKVSNPKLKVQTVRLFDESLNETDKEPVYAAGGVNQYRPRLNNGENLSFIDYG
                     NGKLILSNNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRL
                     SKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVSGRGTVQLNA
                     DNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNATTTSTVTITGNESI
                     TQPSGKNINRLNYSKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNG
                     NITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQG
                     GQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKV
                     IASLTKTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGNLSLVGN
                     AQATFNQATLNGNTSASGNASFNLSNNAAQNGSLTLSDNAKANVSHSALNGNVSLADK
                     AVFHFENSRFTGQLSGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAA
                     GAQTGSVSDTPRRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRS
                     DKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGA
                     WRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKT
                     ESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARR
                     DLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVW
                     TSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNS
                     ARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGGKIRRRVLHYGIQARYRAGFG
                     GFGIEPYIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPY
                     LSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLE
                     AQHSAGIKLGYRW"
     gene            complement(6937..7098)
                     /locus_tag="WGO87_001422"
                     /pseudo
     CDS             complement(6937..7098)
                     /locus_tag="WGO87_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1947 a         2194 c         1868 g         1588 t
ORIGIN      
        1 accaaacgga tcggattccc gtttttatgg gataacggaa tgttcagccg gacaccgcca
       61 cgatgctttc gatccgttta ttttccgatg cagaatctcg aaaaaatcac gccgagcagg
      121 tcgtccgccg taaactcgcc cgtgatttcg ccgcatgcga cctgagccaa gcgcaagtgt
      181 tcggcaaaca gctcgatttg atggttgccg cacaatgccg ccagcgacaa ttcttcctgc
      241 gctgctttga gtgcgttgac gtgccgcgtc cgcgccaaaa acagcccttc gctttcgccc
      301 tgccaaccgg cctcacgcaa caacgtccgt ttcagcgcgt ccaagccgtc gccagttttc
      361 gccgacagcg cgatgacggt ttccgcgccc gtaccgaacc cgcctgccgc gtgtgcgtgc
      421 aaatcggatt tgctgtggat ttcgatgcgt ttcaactccg gcggcaacgc gtccaaaatc
      481 gcccgtgtct tttcattcaa accctcgcgc ggatcgacca acaccagcgc gacatcggct
      541 tcggatacgg ctttgcggct gcgttcgatg ccgatgcgtt cgaccacgtc gtccgtctcg
      601 cgcaaacctg ccgtatcgac aatatgcacc ggcacgccgt caatcaggat acgttccctg
      661 accgcgtcgc gcgtcgttcc ggcaatatcg gtaacaatcg ccacttcgtc gcccgccagc
      721 gcgttcaaca ggctggattt gccgacattc ggcgcgccga ccaatacgac attcagacct
      781 tcgcgcaaaa tcgcgccctg ctgcgcgttg gcaagcacat catccacggc gcggcgcaag
      841 ccgtccagtt tgccgcgtgc gtctgccgct tcgagaaaat caatgtcttc ctcgggaaaa
      901 tccagcgtcg cttcgaccag catccgcaag gtaatcaaat cttcgaccag accgtgtatc
      961 cgccgcgaaa aatcgccctt gagcgagcgc aacgccagac gcgccgccga acggctggat
     1021 gcgtcaatca aatccgccac gccttccgcc tgtgccaagt ccagtttgtc gttcaaaaac
     1081 gcacgtttgg taaactcgcc cggttcggca aggcgcgcgc ccaattccaa acagcggttc
     1141 agcagcatat ccatcaccac cggcccgccg tgtccctgaa gctcgataac atcttcgccc
     1201 gtaaaacttg ccggtgcggc aaaaaacagc aaaagcccgc tgtcgattgc ctgaccgtcc
     1261 gcgtccgtaa aatcagcata ggttgcggta cgcggtttgg gcgttttccc acacaaagcc
     1321 tctgccatcg gcagcaggtt tttccccgat atgcgtatca cgcccacgcc gccgcgcccg
     1381 ggtgcggtag cgactgccgc aatcgttgga acgttatccg acataaaacc cccgaaaatt
     1441 caaaacagcc gcgattatag caaatgccgt ctgaagtccg acggtttggc tttcagacgg
     1501 tataaaaccg caaaaatgct tgataaatcc gtccgcctga cctaatataa ccatatggaa
     1561 aaacgaaaca catacgccct cctgctcggt ataggctcgc tgttgggcct gttccatccc
     1621 gcaaaaaccg ccatccgccc caatcccgcc gacgatctca aaaacatcgg cggcgatttt
     1681 caacgcgcca tagagaaagc gcgcaaatga ccgaaaacgc acaggacaag gcgcggcagg
     1741 ctgtcgaaac cgtcgtcaaa tccccggagc ttgtcgagca aatcctgtcc gacgagtacg
     1801 tgcaaataat gatagcccgg cgtttccatt cgggaccgtt gccgccgccg tccgacttgg
     1861 cgcaatacaa cgacattatc agcaacgggg cagaccgcat tatggcaatg gcggaaaaag
     1921 aacaagccgt ccggcacgaa accatacggc aagaccaaac cttcaacagg cgcgggcaac
     1981 tgtacggctt catcagcgtc atcctgatac tgctttttgc cgtcttcctc gtatggagcg
     2041 gctaccccgc aaccgccgcc tcccttgccg gcggcacagt ggttgccttg gcgggtgctt
     2101 tcgtgattgg aagaagccga gaccaaggca aaaattaatg acaaatccta gggcgtgctt
     2161 catatccgcc cgaacgccga accgcacata taggcacatc ccgcgcgccg cccggaagcg
     2221 gaagccgcgc cctcccaaac aaacccgaat cccgtcagat aaggaaaaat aatgaaaaca
     2281 accgacaaac ggacaaccga aacacaccgc aaagccccga aaaccggccg catccgcttc
     2341 tcgcctgctt acttagccat atgcctgtcg ttcggcattc ttccccaagc ctgggcggga
     2401 cacacttatt tcggcatcaa ctaccaatac tatcgcgact ttgccgaaaa taaaggcaag
     2461 tttgcagtcg gggcgaaaga tattgaggtt tacaacaaaa aaggggagtt ggtcggcaaa
     2521 tcaatgacaa aagccccgat gattgatttt tctgtggtgt cgcgtaacgg cgtggcggca
     2581 ttggtgggcg atcaatatat tgtgagcgtg gcacataacg gcggctataa caacgttgat
     2641 tttggtgcgg aaggaagaaa tcccgatcag caccgttttt cttaccaaat tgtgaaaaga
     2701 aataattata agcctgacaa ttcacaccct tacaacggcg attaccatat gccgcgtttg
     2761 cataaatttg tcacagatgc agaacctgtc gaaatgacga gtgacatgag ggggaatacc
     2821 tattccgata aagaaaaata tcccgagcgt gtccgcatcg gctcaggaca ccactattgg
     2881 cgttatgatg atgacaaaca cggcgattta tcctactccg gcgcatggtt aattggcggc
     2941 aatacacata tgcagggttg gggaaataat ggcgtagtta gtttgagcgg cgatgtgcgc
     3001 catgccaacg actatggccc tatgccgatt gcaggtgcgg caggcgacag cggttcgcca
     3061 atgtttattt atgacaaaac aaacaataaa tggctgctca acggagtttt acaaaccggc
     3121 tacccttatt ccggcaggga aaacggtttc cagctgatac gcaaagattg gttctacgat
     3181 gacatttaca gaggcgatac acataccgtc ttttttgaac cgcgcagtaa cggacatttt
     3241 tcctttacat ccaacaacaa cggtacgggt acggtaacag aaaccaacga aaaggtttcc
     3301 aatccaaagc ttaaagtaca gacagtccga ctgtttgacg aatctttgaa tgaaactgat
     3361 aaagaaccag tttacgcggc agggggtgtt aatcagtacc gtccaaggtt aaacaacggt
     3421 gaaaaccttt cttttatcga ttacggcaac ggcaaactca tcttatcaaa caacatcaac
     3481 caaggcgcgg gcggtttgta ttttgaaggt gattttacgg tctcgcctga aaacaacgaa
     3541 acgtggcaag gcgcgggcgt tcatatcagt gaagacagta ccgttacttg gaaagtaaac
     3601 ggcgtggcaa acgaccgcct gtccaaaatc ggcaaaggca cgctgcacgt tcaagccaaa
     3661 ggggaaaacc aaggctcgat cagcgtgggc gacggtacag tcattttgga tcagcaggca
     3721 gacgataaag gcaaaaaaca agcctttagt gaaatcggct tggtcagcgg caggggtacg
     3781 gtgcaactga atgccgataa tcagttcaac cccgacaaac tctatttcgg ctttcgcggc
     3841 ggacgtttgg atttaaacgg gcattcgctt tcgttccacc gtattcaaaa taccgatgaa
     3901 ggggcgatga ttgtcaatca taatgccaca acaacatcca ccgttaccat tacagggaat
     3961 gaaagtatta cacaaccgag tggtaagaat atcaatagac ttaattacag caaagaaatt
     4021 gcctacaacg gttggtttgg cgagaaagat acgaccaaaa cgaacgggcg gctcaacctt
     4081 gtttaccagc ccgccgcaga agaccgcacc ctgctgcttt ccggcggaac aaatttaaac
     4141 ggcaacatca cgcaaacaaa cggcaaactg tttttcagcg gcagaccgac accgcacgcc
     4201 tacaatcatt taggaagcgg gtggtcaaaa atggaaggta tcccacaagg agaaatcgtg
     4261 tgggacaacg actggatcaa ccgcacgttt aaagcggaaa atttccatat tcagggcggg
     4321 caggcggtga tttcccgcaa tgttgccaaa gtggaaggcg attggcattt gagcaatcac
     4381 gcccaagcag tttttggtgt cgcaccgcat caaagccata caatctgtac acgttcggac
     4441 tggacgggtc tgacaaattg tgtcgaaaaa accattaccg acgataaagt gattgcttca
     4501 ttgactaaga ccgacatcag cggcaatgtc agccttgccg atcacgctca tttaaatctc
     4561 acagggcttg ccacactcaa cggcaatctt agtgcaaatg gcgatacacg ttatacagtc
     4621 agccacaacg ccacccaaaa cggcaacctt agcctcgtgg gcaatgccca agcaacattt
     4681 aatcaagcca cattaaacgg caacacatcg gcttcgggca atgcttcatt taatctaagc
     4741 aacaacgccg cacaaaacgg cagtctgacg ctttccgaca acgctaaggc aaacgtaagc
     4801 cattccgcac tcaacggcaa tgtctcccta gccgataagg cagtattcca ttttgaaaac
     4861 agccgcttta ccggacaact cagcggcagc aaggatacag cattacactt aaaagacagc
     4921 gaatggacgc tgccgtcagg cacggaatta ggcaatttaa accttgacaa cgccaccatt
     4981 acactcaatt ccgcctatcg ccacgatgct gcaggcgcgc aaaccggcag tgtgtcagac
     5041 acgccgcgcc gccgttcgcg ccgttcccta ttatccgtta caccgccaac ttcggtagaa
     5101 tcccgtttca acacgctgac ggtaaacggc aaattgaacg gtcaaggaac attccgcttt
     5161 atgtcggaac tcttcggcta ccgaagcgac aaattgaagc tggcggaaag ttccgaaggc
     5221 acttacacct tggcggtcaa caataccggc aacgaacccg taagcctcga tcaattgacg
     5281 gtagtggaag ggaaagacaa caaaccgctg tccgaaaacc ttaatttcac cctgcaaaac
     5341 gaacacgtcg atgccggcgc gtggcgttac caactcatcc gcaaagacgg cgagttccgc
     5401 ctgcataatc cggtcaaaga acaagagctt tccgacaaac tcggcaaggc agaagccaaa
     5461 aaacaggcgg aaaaagacaa cgcgcaaagc cttgacgcgc tgattgcggc cgggcgcgat
     5521 gccgccgaaa agacagaaag cgttgccgaa ccggcccggc aggcaggcgg ggaaaatgtc
     5581 ggcattatgc aggcggagga agagaaaaaa cgggtgcagg cggataaaga cagcgccttg
     5641 gcgaaacagc gcgaagcgga aacccggccg gctaccaccg ccttcccccg cgcccgccgc
     5701 gcccgccggg atttgccgca accgcagccc caaccgcaac ctcaacccca accgcagcgc
     5761 gacctgatca gccgttatgc caatagcggt ttgagtgaat tttccgccac gctcaacagc
     5821 gttttcgccg tacaggacga attggaccgc gtgtttgccg aagaccgccg caacgccgtt
     5881 tggacaagcg gcatccggga caccaaacac taccgttcgc aagatttccg cgcctaccgc
     5941 caacaaaccg acctgcgcca aatcggtatg cagaaaaacc tcggcagcgg gcgcgtcggc
     6001 atcctgtttt cgcacaaccg gaccgaaaac accttcgacg acggcatcgg caactcggca
     6061 cggcttgccc acggcgccgt tttcgggcaa tacggcatcg gcaggttcga catcggcatc
     6121 agcacgggcg cgggttttag cagcggcagt ctttcagacg gcatcggagg caaaatccgc
     6181 cgccgcgtgc tgcattacgg cattcaggca cgataccgcg ccggtttcgg cggattcggc
     6241 atcgaaccgt acatcggcgc aacgcgctat ttcgtccaaa aagcggatta ccgctacgaa
     6301 aacgtcaata tcgccacccc cggtcttgcg ttcaaccgct accgcgcggg cattaaggca
     6361 gattattcat tcaaaccggc gcaacacatt tccatcacgc cttatttgag cctgtcctat
     6421 accgatgccg cttcgggcaa agtccgaaca cgcgtcaata ccgccgtatt ggctcaggat
     6481 ttcggcaaaa cccgcagtgc ggaatggggc gtaaacgccg aaatcaaagg tttcacgctg
     6541 tccctccacg ctgccgccgc caaaggcccg caactggaag cgcaacacag cgcgggcatc
     6601 aaattaggct accgctggta accgccggat atgccgaaag gggtctgacg atgccaccgt
     6661 gcgctgtcaa accctttttc tgccgccgcc ttgtgcctgc tccattgtct gataaccgtc
     6721 aaattgattt ttagcccatg tttggtgggt cggggaaatc tatatcttcg tccgtgccga
     6781 aatagtctga gacctttgca aaattccttt ccctcccgac agccgaaacc caaacacagg
     6841 ttttcgtcta ttttcgcccc aaatacctcc taattctacc caaatacccc cttaatcctc
     6901 cacggacacc cgataatcag gcatccgggc tgctttttag gcggcagcgg gcgcacttag
     6961 cctgttggcc gctttcaaaa ggttcaaaca catcgccttc agatggcttt gtgcactcac
     7021 tttgctcagc ccgaaatagg ctgcccgcgc atagcggaat ttacggtgca gcgtaccgaa
     7081 gctctgttcg accacatata gtggattaaa tttaaaccag tacggtgttg cctcgccttg
     7141 ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttaaat ttaatccact
     7201 ataacgggtc ttcgataaat atcggttacg tttggtttgc gtttccgtca gcggacggtt
     7261 gcggcaggct ttgcgcataa tgccgttctg caaccgatgt tctttcagat gttgccggtt
     7321 ttccttgctg tcgtagcctt tgtcggcata gacggtcgta cctttgggca gtccttccaa
     7381 caacggcgac aggtgtgtgc actcatgggc attggtggga gtgatgtaca gtttctcgat
     7441 atagccttcc gcatcggtac gggtatgttg tttgtaaccg agtttgtaga ggctgttttt
     7501 ctttgtccaa cgggcatttt tgtccttact cagtgtggtt tgaccgctga cttgtccctc
     7561 ttcatcgact tctatggcct gacgctgttc gctgccg
//
LOCUS       DAWFBO010000095         7546 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFBO010000095 DAWFBO010000000
VERSION     DAWFBO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.095"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            24..419
                     /locus_tag="WGO87_001423"
     CDS             24..419
                     /locus_tag="WGO87_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0392775.1"
                     /translation="MFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAGHGI
                     LYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLF
                     AAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WGO87_001424"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WGO87_001424"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0392776.1"
                     /translation="MFQKHLQKASGSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKVIGCLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIAATLADMAELFPERRLMLAREITKTFETFLSGTPGELQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1375..1722
                     /locus_tag="WGO87_001425"
     CDS             1375..1722
                     /locus_tag="WGO87_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0392777.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGIILFVEVKYRKNGRFGGSAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIKNITG"
     gene            1727..2320
                     /locus_tag="WGO87_001426"
     CDS             1727..2320
                     /locus_tag="WGO87_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0392778.1"
                     /translation="MTTLPERVAAHFAESIRAKQEAGEVLAEPAAQAAEMMLQCLMND
                     GKILICGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDYIF
                     SKQVRALGRAGDILVGISTSGNSANVAEAVKAAHEHDMRVVALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2384..2992
                     /locus_tag="WGO87_001427"
     CDS             2384..2992
                     /locus_tag="WGO87_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0392779.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3000..3644
                     /locus_tag="WGO87_001428"
     CDS             3000..3644
                     /locus_tag="WGO87_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392780.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3684..4463
                     /gene="map"
                     /locus_tag="WGO87_001429"
     CDS             3684..4463
                     /gene="map"
                     /locus_tag="WGO87_001429"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0392781.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4618..4917
                     /locus_tag="WGO87_001430"
     CDS             4618..4917
                     /locus_tag="WGO87_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0392782.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5155..5529
                     /locus_tag="WGO87_001431"
     CDS             5155..5529
                     /locus_tag="WGO87_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0392783.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5717..7183)
                     /locus_tag="WGO87_001432"
     CDS             complement(5717..7183)
                     /locus_tag="WGO87_001432"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0392784.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1923 a         2296 c         1858 g         1469 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcgccgcgcg attggttgcc
      661 gtccgccgcc aatgccgtct gaagctcccc cggcgtgccg cttaagaacg tttcaaacgt
      721 tttggtgatt tcgcgcgcca gcattaatcg gcgttcgggg aacagttccg ccatatcggc
      781 aagcgtcgcc gcaattcggt gcggcgtttc aaacatcacg acgggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgttctcc cgatttcggc ggcacaaaac cgttgaaata
      901 aaaatcggat ccctccacac cggccacgct caaagccgcc attaccgcgc ttgcgcccac
      961 gacggggaca actttaaacc ccgcctcgcg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacagcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagaca
     1081 gccgataacc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga cgagtttgcc
     1141 ctgaatgccg tacgcactca aaagctgcgc agtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcacg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgccg gaggctttct gcaagtgttt
     1321 ctgaaacata agaatgccgt ctgaaaaaca aacattataa aggttaaacc gattatgcgc
     1381 ttaaaccaca aacagggcga ggcaggcgaa gatgccgcgc ttgccttcct ccaatcccaa
     1441 ggctgcacgc tgcttgcccg caactggcac tgcgcctacg gcgaaatcga cctgattgtc
     1501 aaaaacggcg gcataatact gttcgttgaa gtaaaatatc gcaaaaacgg gcggttcggc
     1561 ggttcggcat acagcatttc gccgtccaaa ttattgaaac tgcaacgaag tgtagagtat
     1621 tatctgcaac agaacaggct gaccaacgta ccgtgccgcc tcgacgcggt actcatcgaa
     1681 ggcagccgac cgcccgagtg gataaagaat attacaggtt gacgatatga cgacattacc
     1741 agaacgcgtt gccgcccatt ttgccgaaag catccgcgcc aaacaggaag ccggggaagt
     1801 gctcgccgaa cccgccgcgc aggctgccga gatgatgctg caatgcctga tgaacgacgg
     1861 caaaatcctg atatgcggca acggcggttc ggctgccgac gcacagcatt tcgccgccga
     1921 aatgaccggg cgttttgaaa aagaacgtat ggaactcgcc gccgtcgccc tgaccaccga
     1981 cacgtccgcc ctgaccgcca tcggcaacga ctacggcttc gattatatct tcagcaaaca
     2041 agtgcgcgcc ctcggacgcg ccggcgacat cctcgtcggc atttccacct ccggcaattc
     2101 cgccaacgtc gccgaagccg tcaaagccgc ccacgaacac gatatgcgcg tcgtcgcact
     2161 gaccggccgc gacggcggca aaatcgccgc gatgctcaaa gacaccgacg tactgctcaa
     2221 cgtcccccat ccgcgcaccg cccgcattca ggaaaaccac atcctgctga tacacgccat
     2281 atgcgactgt atcgactccg tactgctgga aggaatgtaa cccttttcag acggcatggc
     2341 gcaaagcaat gccgtctgaa acgcccaaga aaggaagccc ccgatgaaac ccaaaccgca
     2401 caccgtccgc accctgattg ccgccatttt cagccttgcc cttagcggct gcgtcagcgc
     2461 agtaatcgga agcgccgccg tcggcgcgaa atccgccgtc gaccgccgaa ccaccggcgc
     2521 gcaaaccgac gacaacgtta tggcgttgcg tatcgaaacc accgcccgct cctatctgcg
     2581 ccaaaacaac caaaccaaag gctacacgcc ccaaatctcc gttgtcggct acaaccgcca
     2641 cctgctgctg ctcggacaag tcgccaccga aggcgagaaa cagttcgtcg gtcagattgc
     2701 acgttccgaa caggccgccg aaggcgtata caactacatt accgtcgcct ccctgccgcg
     2761 cactgccggc gacatcgccg gcgacacttg gaacacatcc aaagtccgcg ccacgctgtt
     2821 gggcatcagc cccgccacac aggcgcgcgt caaaatcgtt acctacggca acgtaaccta
     2881 cgttatgggc atcctcaccc ccgaagaaca ggcgcagatt acccaaaaag tcagcaccac
     2941 cgtcggcgta caaaaagtca tcaccctcta ccaaaactac gtccaacgct gactcggcaa
     3001 tgccgtctga accgccttca gacggcattg cccgacaccc caaaagcaca atcaaaatgg
     3061 caaaaaaacc gaacaaaccc ttcaggctga cccccaaact cctgatacgc gtcgtattgc
     3121 tcatctgtat caccgccatc ggcgcattgg caataggcat cgtcagcaca ttcaacccga
     3181 acggcggcaa aaccctccaa accgaaccgc aacacaccga cagcccccgc gaaaccgaat
     3241 tctggctgcc aaacggcgta gtcggacaag atgccgccca acccgaacac caccacgccg
     3301 cctcatccga acccgcacag ccggacggca cagacgaaag cggcagcgga ctgccgtccc
     3361 ctgccgcacc caagaaaaac cgggtcaaac cgcaacctgc cgacacagct caaaccgaca
     3421 ggcagccgga cgacgccgga acacaagctg aaaacacact caaagaaacc cccgtactgc
     3481 ccacaaacgt cccccgtccc gaaccccgaa aagaaacacc cgaaaaacag gcgcagccca
     3541 aagaaacgcc caaagaaacg cccaaagaaa accataccaa accggacacc ccgaaaaaca
     3601 cgccgcccaa accccataaa gaaattctcg acaacctctt ctgacccggc acggcaggca
     3661 cacccgcaat ccaaggaagc attatgaacg gcatcatcat caaaaccccc gaagaaatcg
     3721 aaaaaatgcg cgagctgggc aaactcgtcg ccgaagccct cgactacatc ggacaattcg
     3781 tcaaacccgg cgtaaccacc gacgaaatcg acaaactcgt ttacgactac cacgtcaacg
     3841 tccaaggcgg ctatcccgcc cccctgcact acggcaaccc gccctacccc aaatcctgct
     3901 gcacctccgt caaccacgtc atctgccacg gcattcccga cgacaagccg ctcaaagaag
     3961 gcgacattat caacatcgac ctcaccatca aaaaagacgg cttccacggc gactccagcc
     4021 gtatgtttac cgtcggcaaa gtctccccca tcgcccaacg cctgatcgac gtaacccacg
     4081 cctccatgat ggcgggcata gaagccgtca aacccggcgc gacactgggc gacgtaggtt
     4141 acgcctgcca acaggttgcc gaaaacgccg gctattccgt cgtacaggaa ttctgcggac
     4201 acggcatcgg gcgcggtttc cacgaagccc cgcaagtgtt gcactacgga aaaaaaggac
     4261 agggctccgt tctaaaaccg ggtatgattt ttaccgtcga accgatgatc aaccaaggca
     4321 aacgccacct gcgtatcctc aacgacggct ggacggtggt taccaaagac cgctccctct
     4381 ccgcccaatg ggaacacgaa gtcttggtga ccgaaaccgg ctacgaaatc ctcaccgtca
     4441 gccccgcctc cggcaaaccc tgaagccgga cgtatccgcc ccataaaaac aaacaatgcc
     4501 gtctgaaaga aacggcagat atgatatata atataaaaac aggcttgacc cggcacatta
     4561 cgaaaacaaa gcaaatcgga atttgccccg caaccagaca aacttaaagg aagttttatg
     4621 aaaatatttg aaaatataga agatgttaaa gccatccgta aaaagaccgg gctgaaccag
     4681 atagacttct ggggcaaggt cggcgttacc cagtctggag gctcgcgcta cgaaaccggc
     4741 cgcaaaatgc ccaaacccgt acgcgaactg ctccgcctcg tccatatcga atgcatcgat
     4801 ttggcgaaag tcaacaaaaa agatatggaa atcgccgccc tgttgaaaaa acaccatccc
     4861 gacctgtatg ccgagttgtc caaacagacc aagtccgaaa gaaaaaaaca aagttaaacc
     4921 gcaacctccg gatgcccgac agtttttcat ttccgaaaaa cgcaaacaat gccgtctgaa
     4981 acaccggaca ggccgccgta tcccgcctgc cgcccctgcc tcaaaccgcc gaaccgcccg
     5041 aacccgcctt tttacaaact ttatccaatt tcctgtttat ttcaggatac gccgacatta
     5101 gaatgtcaaa cagctcgaaa cgggcaaact ccacatccat ccaaaggaat aaaaatgaaa
     5161 cttctgacca ccgcaatcct gtcttccgca atcgcgctca gcagtatggc tgctgctgcc
     5221 ggcacgaaca accccaccgt tgccaaaaaa accgtcagct acgtctgcca gcaaggtaaa
     5281 aaagtcaaag taacctacgg cttcaacaaa cagggtctga ccacatacgc ttccgccgtc
     5341 atcaacggca aacgtgtgca aatgcctgtc aatttggata aatccgacaa tgtggaaaca
     5401 ttctacggca aagaaggcgg ttatgttttg ggtaccggcg tgatggatgg caaatcttac
     5461 cgcaaacagc ccattatgat taccgcacct gacaaccaaa tcgtcttcaa agactgttcc
     5521 ccacgttaat caggcaacaa aaaacagcgt tttcagaaat gaaaacgctg tttttttgac
     5581 cgttccatta ttcgcaaaag ggaaaaaacg attacctgcc ccgtgtatca aaacctgccc
     5641 tgccggatga agggcataac cggcagggac ggcgtcaaca ccatatgggg gtacggcttt
     5701 tcttgaaaga ttcggcttaa atatccaata ctttcgcggt ataggcgata atttcatccg
     5761 ccctttcagg gttttcgttc aacttgatgc cgtaacccgg taccagctct ttcagacggt
     5821 cttcccaaga cggggcgcgc tcggggaagc attggtgcat cagccggatc atcagcggca
     5881 cagcggtcga tgcgcccggc gacgcgccca gcaatgcggc gagtgagccg tcggcgtggg
     5941 cgacaatctc cgtaccaaac tggagcacgc cgcctttttc ggagtctttt ttaatgattt
     6001 ggacgcgttg ccctgcggtg atgagttccc agtcgtcggg gtttgcctcg gggtagtatt
     6061 ccagcaggga ggcgaagcgt tcttctttgg ttttacgcaa ttcgcccagc aggtatttgg
     6121 tcagcggcat attcgcccag ccggcgcaca gcataggata gaggttgtcc atatggatgg
     6181 acagcggcaa atccataagc gagccttgct tgaggaagtt ggaacggaag cctgcgtaag
     6241 ggccgaacat aaggtggcgt ttgccgtcca cgttgcgtgt gtcgaggtgc gggacggaca
     6301 tcggcggcgc gccgacggaa gcctgcccgt acactttggc gttgtgttgt tcggcggttt
     6361 cggggttgct gttgcggaag aacaggccgg acacggggaa gccgccgtag cctttgcctt
     6421 cggggatgcc ggatttttgc agcagggtca gcgcgccgcc gcccgcgccg aggaagagga
     6481 agcgggtacg gagggtgagc tgcccgtcgg ggttgcgggt atcggcggtt ttgagcaccc
     6541 acgcgccgtc ggattcgcgt ttgatgtctt cgacgtggcg gttgaactcg gtttttacgc
     6601 ccttgccctg caaatatttc accatttggc gcgtcagccg tccgaaatcg acatccgtac
     6661 cttcggcgga gtagttggcg gcgacgggtt ggttttcgtc ccggccgcgc atcatcagcg
     6721 gagcccaatc ggaaattttg ttccgatcgg tggaaaattc catattttca aaaagttttt
     6781 gggttttaaa cgcgtcataa cgtttttgaa gataagaaca atggtcttca ttcatcacca
     6841 aagacatatg cggcacggca ttgatgaagg aattgtcttc caacttgcct tccgcgacca
     6901 gcgtcgccca aaactggcgg ctgacatgaa actgttcggc aatattgagg gcgcgcgccg
     6961 gatcgataat cccatttgca cccaacggcg catagttcaa ttcgcacagc gcggaatgcc
     7021 ccgtgccggc gttgttccac gcgtttgacg attccaacgc cacatcttcc aagcgttcaa
     7081 tcagggtgat ttcccaagac ggttcgagtt ctttgagcaa aacgcccaaa gtcgcgctca
     7141 taatgccgcc gcccaccaag acaacgtctg tcgcttcagc catggtttac tcctaaaaaa
     7201 caggcatctt ctgcccttat ggttatttgc cgtactacaa acgcctgaat cgcaaaagca
     7261 gggaaaaccg gcaatggtgt gtgtccgagt atgctgtttc ggggttggaa tgcgttgcaa
     7321 gtatggcttc cgacaccgct tcaggggctt gtaatatgtt atcgtgaatg tagtggattt
     7381 tactgggaaa tgcaaagttt ttctgtcgcc cgccaagtcg ggaaactgcg aaatgaaaaa
     7441 taaaaatagt tatttatcta tatatatcaa atttttaata gataaaaaat caaaattgtt
     7501 tatatattaa tttttaaaag attgtcagca tattgcgtta agtttt
//
LOCUS       DAWFBO010000096         7453 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFBO010000096 DAWFBO010000000
VERSION     DAWFBO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.096"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            155..328
                     /locus_tag="WGO87_001433"
     CDS             155..328
                     /locus_tag="WGO87_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0392785.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
     gene            392..1675
                     /gene="hemL"
                     /locus_tag="WGO87_001434"
     CDS             392..1675
                     /gene="hemL"
                     /locus_tag="WGO87_001434"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0392786.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGSAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAE"
     gene            complement(1891..2163)
                     /locus_tag="WGO87_001435"
     CDS             complement(1891..2163)
                     /locus_tag="WGO87_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ0392787.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2174..2737)
                     /gene="orn"
                     /locus_tag="WGO87_001436"
     CDS             complement(2174..2737)
                     /gene="orn"
                     /locus_tag="WGO87_001436"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0392788.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERTTP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2755..3642)
                     /gene="prmA"
                     /locus_tag="WGO87_001437"
     CDS             complement(2755..3642)
                     /gene="prmA"
                     /locus_tag="WGO87_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0392789.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGETIEDQDWVR
                     LTQAQFDPIRISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            complement(3760..5121)
                     /gene="accC"
                     /locus_tag="WGO87_001438"
     CDS             complement(3760..5121)
                     /gene="accC"
                     /locus_tag="WGO87_001438"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0392790.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEEERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5234..5689)
                     /locus_tag="WGO87_001439"
     CDS             complement(5234..5689)
                     /locus_tag="WGO87_001439"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0392791.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5671..5928)
                     /locus_tag="WGO87_001440"
     CDS             complement(5671..5928)
                     /locus_tag="WGO87_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0392792.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6045..7085
                     /gene="queA"
                     /locus_tag="WGO87_001441"
     CDS             6045..7085
                     /gene="queA"
                     /locus_tag="WGO87_001441"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0392793.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         1551 a         1875 c         2217 g         1810 t
ORIGIN      
        1 gacggaagag ggaatcgcac aaacggttgt taaaggcaaa acgactcggg cggaggtaga
       61 ggcacgtttc gggaagcgca accctttcgg ttgttatgcc tatcatgaag tcagcctgcc
      121 gatttataat tttttgccga ccaatttcat ttatatgaaa tcagaacgcc ggcattggga
      181 gtggtgtgtg gattacgact gggaaggagt tgtccgggac taccgcttta cacataaagt
      241 ggaaaaagac gagcgttccg tcatccggga tgcggttggc gtcatccgca aagaagcagg
      301 caaatccttg tcgcaacctg aaaaatgata aaatgggact ttctgcttct aagcccgaaa
      361 cctgccgttc agacggcatt tgaggataaa tatgaaccgt aacgaaattt tattcgaccg
      421 cgccaaagcc atcatccccg gcggcgtgaa ttcccccgtc cgcgcattcg gcagcgtcgg
      481 cggcgtaccg cgcttcatca aaaaagccga aggcgcgtat gtttgggatg aaaacggcac
      541 gcgctacacc gactatgtcg gctcgtgggg gtctgcgatt gtcggccacg cgcaccccga
      601 agtcatcgaa gccgtgcgcg aagccgcgtt gggcggtttg tcgttcggcg cgcctaccga
      661 gggcgaaatc gtcatcgccg aagaaatcgc caaaatcatg ccgtccgtcg aacggctgcg
      721 cctcgtcagc tccggtaccg aagcgaccat gaccgccatc cgtctggcac gcggctttac
      781 cggccgtgac aaaatcatca aatttgaagg ctgctaccac ggccattccg acagcctgtt
      841 ggtgaaagca ggcagcggtc tgctcacctt cggtaatccc tcttccgccg gtgtgcctgc
      901 cgactttacc aaacacactt tagtcctcga atacaacaac atcgcccaac tcgaagaagc
      961 ctttgctcaa agcggcaatg acattgcctg tgtgattttg gagccttttg tcggcaatat
     1021 gaatctggtt aggccgtctg aagccttcgt caaagccctg cgcgaattga ccgaaaaaca
     1081 cggcgcagtg ttgatttacg acgaagtgat gaccggtttc cgcgtcgcgc tcggcggcgc
     1141 acaatcgctg cacggcatca cgcccgacct gaccacgatg ggcaaagtca tcggcggcgg
     1201 tatgccactt gccgcgttcg gcggacgcaa agacattatg gaatgtattt ccccgctggg
     1261 cggcgtgtat caggcaggca ccttgtcggg caacccgatt gccgtcgccg ccggtttgaa
     1321 aacgctggaa atcatccgcc gcgaagggtt ttatgaaaac ctgacggcgc gtaccgaaca
     1381 gttggttcaa ggttttcgga cggcagcgga tgcggcgggc atcgagttca ccgccgacag
     1441 cgtgggcggt atgttcggtc tgtatttcgc cgcacacgcg ccgcgaaact atgccgatat
     1501 ggcgcgttcc aatattgaag gcttcaaaca gtttttccac gggatgctcg accgcggcat
     1561 tgcctttggc ccgtccgctt atgaagcagg tttcgtttcc gccgcgcata cgcccgagct
     1621 gattgacgaa acggttgcgg ttgcggttga agtgttcaag gcgatggctg aatgatgttt
     1681 tgacggacag ggtttctctg ttcgatctgt ttggcagatt gaagtaagaa tgcacaccgc
     1741 cgtcattccc gcgcaggcgg gaatctagac ctttcagttt ctgtaatgat tgaaaataac
     1801 gggccgaaat cagccgcgcc cggcaggaag ccgccgccac ctgaaacaat gccgtctgaa
     1861 gccttcagac ggcattttat cggaagcgca tcagaaaaaa cggatagcga catatgcggt
     1921 aaaagcaagg gcaattacat agataaaccc ctttataaat tcaaaagtct catctatatc
     1981 gctatatttt tctttcagtt cgtcagattt tcgataacgt ttcattttca agaccacttc
     2041 atgaccaaca agcccgacct tccggattcc cgcctgcgcg ggaatgacgg gcgtgtatat
     2101 ttttgatttc aatctgctgt aaaaatgccg tctgaaatgc aggatttgcg cttcagacgg
     2161 cattttttgt ttcttattgc gcttcggctc tcggggcgga aatcagaaag tgttcgcggt
     2221 agtggcgcat ttcttcgatg ctctccaaaa tgtcgtccaa tgccttgtgc gaaccgcgtt
     2281 tgaccacgct tttggcaacg ggcggattcc agcgtttggc aagctctttg agcgtggaaa
     2341 cgtcgaggtt gcggtagtgg aagtagtttt ccagtttcgg catatatttg accataaaac
     2401 gccggtcttg gtggatggaa ttgccgcaca tcggcgtggt gcgttcgggt acccattccg
     2461 acataaagtc cagcagtttc tgttcgactt cggcttcggt atgcgacgat tcgcgtacgc
     2521 gctgcgtcag ccccgtcctg ccgtgtgtgg cggtgttcca ctcgtccata ttgtcgagca
     2581 gctcgtcact ttggtggacg gcgtaaactt cggattgcgc caatacattc aaatccgagt
     2641 cggtaataat catcgcgact tcgataatgc ggtcggtttc gggattaagc cccgtcattt
     2701 ccatatcgag ccagcagagg ttgtttttat cctgcacggt gtttcctttc cgtttcagcg
     2761 ttttacgccg ctcaatcgcg cccatccttc ttcggtttcc gcagggtcga ggtcgaacca
     2821 ttggctgtaa atgccgccga gttcttcggc ttgttcgtcc aacaaaccgg ataacacgat
     2881 gcggccgcct tgtttggtgc gggcggcgag catttcgccg agcatacgta aagggttggc
     2941 gaggatgttg gcgacaacta cgtcgaattg cccttgaggc agaccgtcgg gcaggaagaa
     3001 ttgtgcatcg acgttgttct gttcggcgtt gtccttgccg gcgcggacgg cttgttcgtc
     3061 aatatccacg ccgacggcag aacctgcgcc gagtttgagg gcggcgatgg tcaggatgcc
     3121 cgaaccgcag ccgtagtcga ggacgctttc gccgtttttg agttgcgtat ccagccattt
     3181 gaggcagagg cgcgtggtcg ggtggctgcc ggtgccgaag gcgaggccgg gatcgaggcg
     3241 taggttgacg gcagtgcctt cgggggcttc gtgccaagag ggggtaatcc acaggcgttc
     3301 ggaaattcgg atggggtcga attgcgcttg ggtcaggcgt acccagtctt ggtcttcgat
     3361 ggtttcgccg gtgtatgcca agtcttttaa cccgcattct tgtgcggcgg tttggatgat
     3421 ggcggcggct tcgtcgtgtt cgccgaacag ggcgatgact ttgctctgct gccagatttg
     3481 ttcggcgggc ataccgggtt cgccgaaaat cgcctgttcg ttttgcgtac cggcgtaggc
     3541 atcttcgatg gcggcggaga gtgcgccgtg ttccatcagc gcgtcggcga ggcgttcggc
     3601 gacggcatcg ttgacgttga cggtgatttg ttggtagggc atagcgggct ttcttaacag
     3661 accttcggaa tagggtcgta ctgtataagg ggatgggtgt gtaacggaaa tgccgtctga
     3721 aaacggacaa actgtttcag acggcatatc ggggacggct tatttgtcct gtttggcttt
     3781 gcgatcttcc agccagtgtt ccaaatagtg gatgctgacg ccgccttttt ggaaacccgc
     3841 atcggcgaac aggtcgcggt gaagcggcgt attggttttg atgccggtta ccgccagctc
     3901 ggcgagtgcg acgcgcattt tcgccattgc ctgttcacgc gttttgccgt gtacgcagat
     3961 tttgccgatc aggctgtcgt agtacggtgg gatgcggtag ccttggtaaa tgtggctgtc
     4021 cacgcggatg ccgaagccgc cgggcaggtg gcagctttca atcggccccg ggctggggat
     4081 gaagttgtac gggtcttcgg cgttgatacg gcactcaaac gcgtggcctt cgacttgaat
     4141 atccttttgt ttgtattgca acggcaggcc ggcggcgatg cgcaattgct cttgtacgat
     4201 gtccacgccg gtgatgagtt cggtaaccgg atgctcgacc tgaacgcgcg tgttcatctc
     4261 gataaagaaa aattcgccgt cttcgtacag gaattcaaac gtacctgcgc cacgatagcc
     4321 gatgcgtttg caggcgtcgg cacaggcgtt gccgattttg gcgcgttctt cttcagtgat
     4381 gaacggagcc ggtgcttcct cgatgacttt ttggtggcgg cgttgcagcg aacagtcgcg
     4441 ctcggcaaga tatacggcgt tgccgtgttc gtcggcaatc acttggattt cgacgtggcg
     4501 cggacgttgc agatagcgtt ccatgtaaac catcgggttg ccgaatgccg cgcctgcttc
     4561 tgccttggtc atttcgacag attggaggag gtcttctttt ttctcgacca cgcgcatacc
     4621 gcgaccgccg ccgccgccag aggctttgat aatgacggga taaccgactt tatcggcgat
     4681 tttgaggatt tcttcgccgt cgtcgggcaa tgcgccgtca gaaccgggga cgcagggtac
     4741 gcctgccgct atcatcgcgt gtttggcgga gactttgtcg cccatcaggc ggatggtgtc
     4801 gggtttcggg ccgataaagg taaagccgga ctgctcgacc tgttcggcga aatcggcgtt
     4861 ttcggcaagg aaaccgtagc ccggatggac agcgtccgcg cagcttactt cggcggcggc
     4921 gataatggcg gggacgttaa ggtagctttg cgcggaagcg gcagggccga tgcacacgga
     4981 ttcgtcggcg agtttgacgt gcaggctgtc tttgtcggcc tcggaatgca cggcgacggt
     5041 ggcaatgccc atttcgcggc aggcacggag tacgcgtaat gcgatttcgc ctcggttggc
     5101 gattaaaact tttttcagca tgatgactgt tcctgtggtt ccggcaaccc gtccgtaaaa
     5161 aaaaggtgcg ggaacgctga agggggaaag catttcagac ggcatcggaa gtttatgccg
     5221 tctgaaaaca ggattatccg atgatgaaga gcggttcgcc gaattcgacg ggcgtaccgt
     5281 tttcgaccaa aatttctttg accgtgccgg atttttcggc ttcgatttcg ttcatcagct
     5341 tcatcgcttc gatgatgcac agcgtgtcgc cggctttaac ctgttggccg acttcgacaa
     5401 aaggcgcggc attcgggccg ggtgcgcggt agaacgtgcc gaccataggt gatttttgcg
     5461 cgtcggacaa atcgcgggcg gcaggtgcgg cggcaggcgc ggatgccgca acgggtgcgg
     5521 cggcaggcgt tacggccggc gcggcggcag gtacgggcgc ggcgtaaacg ggtgcggcgg
     5581 cgatggttcg ggtgatgcgg actttttcct cgccttcggt tacttcgatt tcggcgatac
     5641 ccgattcttc aaccaaatca atcagttttt ttaatttgcg caaatccatt tctgttcctt
     5701 taaaggctgc cggtgttccg gcgggttgtt gcgttggttt ttcagaaaac agccggcttg
     5761 cggcaacggc gcaaaccggg cggaatggtg ttcggatacg gttttcctgt ggtggaaaac
     5821 aaccgtgaga atgtgtttat tttcccgaag ttgtcggtaa atgtccagta aaatatcaaa
     5881 aaacggcggt ttttggggga aatgtgcagg aagtttgatt ttgcgcacaa gatgttccag
     5941 gttaagagtg tttattctaa tctgttggtt tttcggacaa agatgccgtc tgaaaaaggg
     6001 ctaaagtgcg tataatggcg gcttgcccga acgagagtgt aaaaatggat atttcagatt
     6061 ttgattttac cctgcccgaa aagctgattg cccagcatcc gcccgaagtg cgcggcagca
     6121 gccgcctttt ggttgcgttg cccgatatgc cgctgcaaga ccgggtgttc ggcgatttgc
     6181 cggattatgt cgaggcgggc gacgttttgg tgttcaacaa caccaaagtc atgaaggcgc
     6241 ggctgtttgg gcagaaagac agcggcggca ggatcgaagc cctgattgag cgcgtgttgg
     6301 acaaccatac cgcactggcg cacatccgtt cgtccaagtc ccccaagccc ggtatggggc
     6361 tggtgtttga aggcggcatc cgtgccgtga tggtcgggcg tgagggcgaa ctgttctgcc
     6421 tgcgttttga aggcggtcaa accgtttacg aacttttgga acagaacgga cacctgcccc
     6481 tgccgcctta tatcgaacgc gccgccgacg cggacgacga cagccgttat caaaccgttt
     6541 atgccaaata tcagggtgcg gtcgccgcgc cgacggcggg tctgcatttt acggaagaac
     6601 ttttgcgccg cctgaaagac aaaggcgcag taaccgcaga agtaaccctg cacgtcggtg
     6661 cgggaacgtt ccagcccgtg cgtgtggaaa aaatcgaaga acacaaaatg cacagcgaat
     6721 ggtttgaagt gccgtctgaa accgccgccg ccgttgaggc ggcaaaagca cgggggaaca
     6781 aagtctgggc ggtcggcacg acttccatgc gcgccctcga atctgccgcg cgtgcgacgg
     6841 gtcgtctgaa agcggggcag ggcgacaccg atattttcat cacgccgggc taccgtttta
     6901 atgttgtcga caggctggtt accaattttc atttgccgaa atcgacgctg ctgatgcttg
     6961 tcagcgcgtt ttcgggtatg ggtcatatcc gcgccgcgta tcgttatgcg gtcgaacgcg
     7021 aataccgttt cttcagctac ggcgatgcga tggttttggg gcggaacgaa ggggtggtac
     7081 gttagaacag ccccaagtct attgggggaa gacaagtgcc gtctgaacgg acgttcggac
     7141 ggcattcttt tgttgatttg aatgaaagga actgtatgac atagattcag gcgcgagagg
     7201 cgagaaactc agtcattgtt gatgacgcgc aatacgttaa aattcattcc gttttggtcg
     7261 tataaacata ttacatacgc catttttttg tattcaaggc tgcctgaaaa cagtgggcaa
     7321 gaatatctaa cccaacgaaa agctgaaaaa gcaacaaaaa ataatctgca ccttagtcgg
     7381 ttggttagtc caaccttagg ggtgcagacc attcaatctt tcaggctgcc tttgtttgtt
     7441 tataagataa att
//
LOCUS       DAWFBO010000097         7272 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFBO010000097 DAWFBO010000000
VERSION     DAWFBO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.097"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(108..2711)
                     /gene="pepN"
                     /locus_tag="WGO87_001442"
     CDS             complement(108..2711)
                     /gene="pepN"
                     /locus_tag="WGO87_001442"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0392794.1"
                     /translation="MSKIVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLTHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPPEAELWDGT
                     ENIDPLCYHQAHEALLDTIAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2847..3641)
                     /locus_tag="WGO87_001443"
     CDS             complement(2847..3641)
                     /locus_tag="WGO87_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0392795.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4391..5260)
                     /locus_tag="WGO87_001444"
     CDS             complement(4391..5260)
                     /locus_tag="WGO87_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0392796.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAKRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKGSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPGEDAKADAQRMNRFIEDRVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5311..5847)
                     /gene="ruvC"
                     /locus_tag="WGO87_001445"
     CDS             complement(5311..5847)
                     /gene="ruvC"
                     /locus_tag="WGO87_001445"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0392797.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5850..6089)
                     /locus_tag="WGO87_001446"
     CDS             complement(5850..6089)
                     /locus_tag="WGO87_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0392798.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6119..7129)
                     /gene="dusB"
                     /locus_tag="WGO87_001447"
     CDS             complement(6119..7129)
                     /gene="dusB"
                     /locus_tag="WGO87_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0392799.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLIS
                     DPTLRNTKKTLHRSDFADEGGVVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDSAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
BASE COUNT         1614 a         1906 c         1927 g         1825 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataaaacg atggggaagt gatgataaaa
       61 cgtgtgtgta actatatcag acggcattgt ttttctgttt gacggcctca atccaaaatt
      121 ttgccgacga tttcgcccac gtctttcgac aatccttcct gcgcccgaat gcgctgcaat
      181 gcttgtttca ccaagttttt gcggtgcggc tcgagcttat tgcagagatt gaacgcctgc
      241 actaggcggg cggcgacctg cgggttgaag cggtcgattt caatgacttt gtcggcgata
      301 aagcggtagc cgctgccgtc ttctgcgtgg aaatgcggga cgttgcggct gaagctgccg
      361 atgagcgaac gggctttgtt ggggttttcg aggctgaatt tcggatgctg caaggcggtt
      421 tgaacctgtt gcagggtgtc gctgcgacgg cttgagccga cgagggcgaa atatttgtcc
      481 atcaccagcg cgtcgtctga aaacttgtcg gcaaactgcg ccagcaggcg gttgcgcgta
      541 tcgctttcgt tgccgttgac ggcggacagg atgccccatt cgtgggtcat gttttgcgcc
      601 atttcgccgt atttttcggc aacggtttcg atgtgcgcgg ggtcggcgcg caggacgaag
      661 gcgcggcaga cgttgcgcag cgtgcgccag ccggcagctt cggggctgta ctcgtagctt
      721 tggttttcct gcttcgccgc ctgacggttc aattcgtgcc atttcggcag gaagcggacg
      781 gcgattgtat ccaacaaggc ttcgtgcgcc tgatggtagc acaacgggtc gatgttttcc
      841 gtgccgtccc acagctcggc ttcaggcggc acgcccaaaa gcagggcttt gaaggcgttg
      901 tctaagaggt cgtctgaaat gactttttcg acggcggcaa gcagtttttc gtgtttcggc
      961 aacccgatgc cgtctgaaag cgcggcaagg ttggcggcga cggcgcggcg gtagagcgtt
     1021 tgggcggctt cccagcgcgt gaaggcgtcg ctgtcgtggg tgagcaggag cagcaggtcg
     1081 tcgtcgctgt acggatagtt cagatgcact ggcgcgctga acccgcgcag cagcgaggga
     1141 acgacggctt cggttacgct ttcgaactgg aaggtctgtt cggcttcggt cagcagtaac
     1201 acggcttcgg ttgcgcgttt gccctgataa tcgaatgcca ccgcttcgcc gttgcggttc
     1261 agcagcccga ctttgacggg aatcatcatc ggctgcttgt ccgccatatc gggcgtgggc
     1321 ggcacggttt gtttgatggt taactcgaaa ctattgtttt tcagacgacc ttccgcttcc
     1381 aagacgggcg tacccgcctg gctgtaccac aaggcgaact ggtcgagatt gatgccgttc
     1441 gcgtccgcca tcgccgcgcg gaaatcgtcg caggtcacgg cctgcccgtc gtgtcgttgg
     1501 aaatagagct tcatgccttt ttggaagccc tcttcgccga gcaaggtgtg atacatccgc
     1561 accacttccg cgcctttttc ataaacggtc atggtgtaga aattgttcat ctcctcatag
     1621 ctggcggggc gcaccggatg ggcggtcggg cctgcgtctt cggggaactg gtgctggcgc
     1681 agcaggcgga tgttctcgat gcggcgcacg gcgcggctgg cgcggtcgcc ggaaaattct
     1741 tggtcgcgga acacggtcaa cccttctttc agcgaaagct ggaaccaatc gcggcaggta
     1801 acgcggttgc ctgtccagtt gtggaaatac tcgtgtccga ccacggattc gatgccttca
     1861 aaatcggtat cggtggcggt acggctgtcg gcaaggacga acttggtatt aaaaatgttc
     1921 agccccttgt tttccatcgc gcccatattg aaatcgccca cggcgacgac catgaaaata
     1981 tccaagtcgt attccaaacc gaagcgcgtt tcgtcccact tcattgcgtt tttcagcgat
     2041 tccacggcaa agccgacctt gggcttgtcc gcttcggtgg tgtaaaactc gattttgacg
     2101 tttctgccgc tcatggtggt gaaatagtct tccgtgaccg ccaaatcgcc cgcgaccaaa
     2161 gcaaacagat aactcggttt ggcaaacggg tcttcccatt tcacccaatg gcggccgtct
     2221 gaatactcgc cgccgtcgat tttgttgccg ttggagagca aaacgggata gcgttttttg
     2281 tccgcgacga tggtggtcgt gaacttggac atgacatccg gacggtcgat atagaacgtg
     2341 attttgcgga agccctccgg ctcgcactgg gtaaacaggt taccggcgga cgcatacagc
     2401 cccatcagcg atttgttttc cgccggcagg atttcggttt ccacttcgac ggtgaagcgt
     2461 tcggacggca cgtctgcaat cgtcagcgtc tcaccttcca acacataatc cgccgccgcc
     2521 ccgttgattt tgacggacaa gagtttcgcc gaaccgtcca acaccagcgg ctcccctacc
     2581 ctctgcggct caaccgtcaa acgcgacttc acgacggttt gcggttcggc aatatcaaaa
     2641 tgcaggtcgg tttcaaggat gcggtaggca ggcgtttggt aatctttcag ataacggacg
     2701 attttgctca tttttttctt tcaatattgt ttggtttgac tggaaaaggc ttcagacggc
     2761 ataggcaaac cctgcaccga tatgccgtat gaaaccgaaa catcagtgcg gacgcaccgt
     2821 caaacaaccg gttttaattt caatctttat ttccacgctt ggacaaattc tgcccaatgc
     2881 ggtttgtctt ctgtttgggc ggaaagatag tttctgatcc gcttgatttc catttcgtat
     2941 tcgtccgcgt cgaatctgcc gctgaccaga cagaaacgca gatacatcaa atacgtattc
     3001 acggcatcgg tttcacaata attgcggatt tccttcagcc tgcccgcgtg gaacgcatcc
     3061 cacaccttgc tgccgtccat acccagcttg cctggaaaac cgcacagttt cgccatatcg
     3121 tccagcggca cgtttgccct cggctggtaa agcgcgagca aatccatcaa atcgcaatga
     3181 cgttggtggt agcggctgat gtagttgttc cacttgaaat cgcggctgtc gccgaaatcg
     3241 ccgtcaccca tatcccaata gcgcgcggcg ttgatgccgt gtagcagcga acggtaatgc
     3301 agaaccggca ggtcgaaacc gccgccgttc caactgacca gttgcggcgt atgtttttca
     3361 atcaactcga aaaatttggc aatcacgact tcttcgccat cgtccatctc gccgattgta
     3421 ccgacatgga ctttatcctg cccccaacgc atgcagcacg aaatcgccac aatctgatga
     3481 agatgatgct gcataaaatc cccacccgtc tgagcacggc gtttttgctg ggcaaacagc
     3541 accacttcat cgtcgggcag cgaggacggc agctcgtaca atgttcggat accctgcaca
     3601 tcgggtacgg tttcaatatc gaaagccaaa atcgtggtca tgacagcacc ttgtatttaa
     3661 aacagacttg cgcctattgt gtcattaaaa ggcggataaa aaaagaggca accccccaca
     3721 ggattgcccc aatacctcaa atcagagatt tacgcttcac aaacaataca ggctttcgcc
     3781 tgcggctttg cccgcgtagc tcaactctac gccggcaaac ttttgtttca ccgtttccga
     3841 tgaaaccccg accaatcgca agactgaccg gaaaatcctt tcagacggca tttcctgcct
     3901 gtcgtgtaat tccatgtagc gaaatgtacg ccattttcta cgctttgcca agcatttttt
     3961 acaatataaa tgtcaaaaca ttaattttat aaaattgctg aaaatattaa atatatggat
     4021 ttttattttt tatatttcaa taaatataaa tttaattttg atttatattt aaatttaagc
     4081 ataaaatgtc aaatattaaa ggaaacatga aaggcatata ttaaatattt atttataacg
     4141 ctatgttttt aaagaaaatt aattttaata tattaactag attgtctgca tatattcata
     4201 ggtttgcggt atttcttcca aaacctgctt cgaatttccc gaccaagtct taaaaatatt
     4261 gttttttgag atacttaaat agcagcgatt atcaaatgaa atctgttcat ataatctgcc
     4321 attttgcatt taaaaaaaca atcaggagtt tcgactcgaa acgcctgata tgttttgtaa
     4381 ttttacgtag tcagtaaaaa tcggggctgc cttccggacg ggttttaaaa cgcttgtgca
     4441 gccaaaaata ttgttccgga tgttcgcgca ccctgtcttc gataaaacgg ttcatgcgct
     4501 gcgcgtcggc tttcgcgtct tcacccggaa aggatttcca agcagggtag aaatgcaatg
     4561 taaccgtatt gtctgcctcg cggacgggaa tggcgggtat cacttttgca tttgcaagcg
     4621 cggcaatgcg gctcaatccg gtaatcgttg ccgtctgaat accgaaaaaa tccacaaaaa
     4681 ccgaatcgtt gcgtccgaaa tcctgatcgg gcagatacag aaacggcgcg ctgcctttgc
     4741 ggaactgttt gacgagggcg cgcagccctt cggtgcgccc gataaggaaa acgttgtgat
     4801 aacggttgcg gcctttcaaa atctgttcgt ccaatatctt gtttttttgg tgggaataca
     4861 tactaattag cggaacatcc tgattaagcg cgtacaccgc catctcgaac gcggtaaagt
     4921 gcggatacag gatgatgact ttttcccccg ccgccagcgc gtcgtccaaa taatgcttat
     4981 tgcggtaacg caccagcgat ttcaggcgtt tggcaggcgc ataccaatat aagccgtatt
     5041 caagcatcag tttcgccata tgtttgaaat gctgcttcaa tacggtttcg cgcttttttc
     5101 cgtcccactc gggaaagcat tttgccaaat tgatttcgcc gatacggcgg cggggtttga
     5161 ccaaaaggta ggcgagcaaa cccgtcaggt cggcaagttt gtgcagcagc gcaaacggca
     5221 gaaactgcaa aacatacagt acaaaaaata taaatttcat ctcgatacac attttctttt
     5281 cagacggcaa aatacaaatg ccgtctgaaa ctattgaaac ctgccgcgct tgacctgcat
     5341 ccccgaagga ttgagtttgg cggcaagccc gtggttgcgt aaggcgtggg tcagcgcgac
     5401 ggcaagaccg tccgccgcat ccgcctgcgg cgttcccgat aagccgagca tctgcaccac
     5461 catatgctgc acctgttctt ttgccgcctt gcccttgccg actaccgcct gtttgacctg
     5521 caaggccgtg tattccgaaa cgggcagctt atggctgacc aatgccgcca atgccgcgcc
     5581 cctagcctga ccgagcatca gcgtcgatgc cggattgacg ttgacgaaca cctgttccac
     5641 tgccgcctgt tgaggcttgt aaacggtaac gacttcgccg atgtgccgga cgatgacggc
     5701 aatcctgtct gccagaggcg catcggcagg cgttttgatg cagcctgagg caacgtaaaa
     5761 atgatcgcgc cccctgacat cgatgatgcc gaaacccgtt acgcggctgc ccgggtcaat
     5821 gcccaagata cggacggttg ccgacatatt cacagcaaac cgtgttgaat cagtttttta
     5881 cgcaaggtat tgcggttcag tcccaacatg acggaggctt tggactggtt gccgccgcat
     5941 tgctccatca cgcacaccag cagcggtttt tccacctgat gcaataccat atcgtacacg
     6001 ccgcaaggtt cggtaccgtt caggtctttg aaatattgtt ctaaattttg tctgatgcat
     6061 tgggaaatat cgggaagggt atggggcatg attgcacttt caaaggataa tcaagtgttc
     6121 agaaggcatt tgggcggtag gcgcacgccc aactgtcggt tttttcggca agtctttcaa
     6181 gataacctgc aagcatgtcg tattgcgccg ccgcactgtc caagcggttg atttcacgac
     6241 gtgtctgttc gccgtcgggc atttcgtcga tgtaccagcc tatgtgtttg cgtgcgatgc
     6301 gtacgcctgc actatcgccg taaaacgcgt gtatggcgcg gatgtggttc aaaatagcgg
     6361 cggcgcattc tgccaaactc aaggcaggcg gtaaaacacc gtgttcggcg taatgtttca
     6421 aatcgcggaa gaaccacggt ctgccttgcg cgccgcgccc tatcatgatg ccgtcggcgg
     6481 cggtttgttt gaggacggcg gcggcttttt gcggcgaagt gatgtcgccg ttgacccaga
     6541 ccgggatgtt cagacggcat ttggtttcgg caatcaggtc gtaagccgcc tcgcctttgt
     6601 acatttgcgt gcgcgtgcgt ccgtggacgg caagggcggc aatgccgcaa tcttcggcga
     6661 ttttggcgat gacgggcagg tttttgtggt cgtcgtgcca acccaaacgg gttttgaggg
     6721 taacgggtac acctgccgca cggacgacgg cttccaaaat ggcggcaacc agcggctcgt
     6781 tctgcatcag cgcgctaccg gcttggacat tgcagacttt tttagcggga cagcccatgt
     6841 tgatgtcgat aagctgcgcc ccgaggctga cgttgtaacg cgcggcatca gccatctgct
     6901 gcggatcgct gccggcaatc tgcacggcaa cgacaccgcc ttcatcggca aaatcgctgc
     6961 ggtgcagggt ttttttggta tttctgagcg tcgggtcgct gatcagcatt tcgcacaccg
     7021 cccaacccgc gccgaaatcg cggcagaggc ggcggaaggg tttgtcggta atgcccgcca
     7081 tcggtgcaag tgcgatgggg ttgtcgataa aatagccgcc gatgtgcata atgggcccgc
     7141 gtttcaaaaa agtgcgacat tgtacatttt ttaagcagga tttccaatct ccggacgcgc
     7201 ccgcgattgg ggcgggcacc gttttatggc ataatccgca cacagatttc ctgccccgcc
     7261 actcacaggc gg
//
LOCUS       DAWFBO010000098         7264 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFBO010000098 DAWFBO010000000
VERSION     DAWFBO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.098"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..821
                     /gene="lpt3"
                     /locus_tag="WGO87_001448"
     CDS             <1..821
                     /gene="lpt3"
                     /locus_tag="WGO87_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ0392800.1"
                     /translation="VKQSYSAGFMTAVSLPSFFNVIPHANGLEQISGGDTNMFRLAKE
                     QGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLGYGNGDNMPDEKLLPLFDKINL
                     QQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDKYDNTIHKTDQMIQTVFEQLQK
                     QPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPLVLYSPNKAVQQAANQAFAPCE
                     IAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGDAGSLSIRDGKAEYVYPQ"
     gene            887..4201
                     /locus_tag="WGO87_001449"
     CDS             887..4201
                     /locus_tag="WGO87_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ0392801.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMCEWRELHHQLAQT
                     AIEMGLTTKETAFRRPPEIRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRTAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPEMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSR
                     QNENHGNTVGWVENPTSAATAKTVGFDNPTYATQQPTPSLAGEGRGEGKTVAAQTNFS
                     ATSVSPLPNPLPQEREQSAAASTVSDGLHPANSQQTAPSLVGEGWGEGKTVAAQTNFS
                     ATSASPLPNPLPQERKQSAAASKVSDDPKPKKRPASPKGRLKPLPLADIRTFEAWLKT
                     AERDNPRLLFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLT
                     LPLTVLNRLHAPSLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDR
                     QAAIIPQLAHFIAKSAGDMRILEQIDQDAWTAQELPEHCYLNLRIIDDGGQELAGGRK
                     LHELQQQLGQAAAVTFRDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKE
                     KDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINA
                     DTLCDDLTQAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAY
                     AELNGKLGKHPLTHLLKLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNP
                     ARDAAREADIQELEQMWQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPY
                     PVSVKRLLKEWERLNK"
     gene            4260..5867
                     /locus_tag="WGO87_001450"
     CDS             4260..5867
                     /locus_tag="WGO87_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0392802.1"
                     /translation="MKSENNYERIVQNSKKFQHSQKNEMQKRITDLLQQLTNGIHERD
                     QIMAVSLLGAIAGHNTFLFGPPGTAKSLISRRLACAFENPQYFEYLMNRFSTPEEVFG
                     PVSIKALKEDRYLRKIEGYLPTADFAFLDEIWKSSPAILNNLLTIINEHIFKNGDERF
                     NVPLKALIAASNEIPPENQGLEALYDRFIIRLLVPPIEQEENFNRLLDSKPSSDKPEI
                     SEDLRISYHELAKWREQLHKVRLSTDTLLIIKYIREELAAQFDKLGVYVSDRRWQRAA
                     VLLKASAFCNGRKETNHSDVILLKYCLWTTPENRESVEQIVMNTIKSCGFDTGIDLAE
                     LDREKESLDKEINKELYHSQDVYETVEMSGKKYFPVQAVFKNVHNSHANKELKIYVPY
                     SKFRSKEKFYPIDSTGNEIQEIECEFDGQGCCKLELKVRGGHWNEQYKPFKFKPNVLF
                     HKGDKKQSINDRLIWSLSRSVMEINKALLDVLKLVTKKHAEHQQFLQSPFITQDDIDI
                     AVSGILDQIDKLNLRIKDCERLESLCR"
     gene            5858..>7264
                     /locus_tag="WGO87_001451"
     CDS             5858..>7264
                     /locus_tag="WGO87_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEZ0392803.1"
                     /translation="MSLNSKLSEQIRWIKASLQIDYADLFKQHGSLNGQIQQRLSDWQ
                     YQTKLALLQKNPFVLHEEKLKKHQQTEIFNQQSVDDYRYFCKLAGVPFEKEFWQQELT
                     QSKNSKPDKQNLPLKLLTEKWQQQLDYAKSQWYLEQLNQLRQEFIDKLKQELDTIRQL
                     AEQLEQFGFEAGLWFDNSIGNLSQQNIEEMKRWLNYLTQDKNAQQIAELLGKMRQIEQ
                     SEKIEQVKQTVYTKSPQIDVNSREEIIGLRLGKDLEYVLPSELALMADEETSTLFDLK
                     FLESKLMCFELQGMTYCDAPTEIIVEQKSQEDEKLGPMILCVDTSGSMVGLPENIAKA
                     MALFLGAKAKSENRSCFVINFSTGIETFELTSKTGISNLIAFLRQSFHGGTDAAPALR
                     HALKMMEQEIYQKADLLMISDFVMNSLPDDLLASIEIQRETGNQFNSLVIGDAFMSKR
                     LKTHFDREWIYNPNVQTIQ"
BASE COUNT         2201 a         1791 c         1634 g         1638 t
ORIGIN      
        1 ttgtgaaaca aagttattcc gcaggcttta tgacggcagt atccctgccc agtttcttta
       61 acgtcatacc gcatgccaac ggcttggaac aaatcagcgg cggcgatacc aatatgttcc
      121 gcctcgccaa agagcagggc tatgagacgt atttttacag tgctcaggct gaaaaccaaa
      181 tggcaatttt gaacttaatc ggtaagaaat ggatagacca tctgattcaa ccgacgcagc
      241 ttggctacgg caacggcgac aatatgcccg atgagaagct gctgccgctg ttcgacaaaa
      301 tcaatttgca gcagggcagg cattttatcg tgttgcacca acgcggttcg cacgccccat
      361 acggcgcatt gttgcagcct caagataaag tattcggcga agccgatatt gtggataagt
      421 acgacaacac catccacaaa accgaccaaa tgattcaaac cgtattcgag cagctgcaaa
      481 agcagcctga cggcaactgg ctgtttgcct atacctccga tcatggccag tatgtgcgcc
      541 aagatatcta caatcaaggc acggtgcagc ccgacagcta tattgtgccg ctggtgttgt
      601 acagcccgaa taaggccgtg caacaggctg ccaaccaggc ttttgcgcct tgcgagattg
      661 ccttccatca gcagctttca acgttcctga ttcacacgtt gggctacgat atgccggttt
      721 caggttgtcg cgaaggctcg gtaacgggca acctgattac gggtgatgca ggcagcttga
      781 gcattcgcga tggcaaggcg gaatatgttt atccgcaata agcgacgtga aaaccaataa
      841 aaacaaattt agatgatgtc ggggaagatg cccgaccgac aagactatgc aaaatatgaa
      901 aaaccaagta cgcggatcag gcatggatgc acgatccaat ccggccaatg tttcagacgg
      961 cctgcaaaac agttcgggtc atatcggtac caacacgcgt taccgcctga ccaaactcgg
     1021 cgaacaaatg gcgcgcctgc ccatcgaccc gaaaatcgcg cgcattttgt tggcggcgaa
     1081 gaaacacgac tgcatggcgg aaatattggt gatcgcgtcc gcactgtcga ttcaagaccc
     1141 gcgcgagcgg ccgctggaag cgcgtgatgc cgccgccaag gcgcacgagc gttttaccga
     1201 caagcaatct gactttcttg cctatctgaa tatttgggac agctttcagc gcgagcgcga
     1261 caaaggtttg tccaacaagc agctggtgca gtggtgccgc caatattttc tgtcgcacct
     1321 gcgtatgtgc gaatggcgcg agctgcacca ccagcttgcc caaaccgcga ttgaaatggg
     1381 tttgaccacc aaggaaaccg ctttcagacg acctcccgaa atcaggcagc tcacgtcgtc
     1441 tgaaaatgcg ggtgaccaag acttatccgc caagctcaaa caaaaacaac tggataaaaa
     1501 gcaacaccgc gcccaaatcc gcaccgccaa agaagcgggc tacgaacaaa tccaccgcgc
     1561 cctgctgacc ggccttatcg ccaacgtcgg catgaaatcg cccgacggca acgactacac
     1621 cggcgcgcgc ggcagccgct tccacctttt ccccgcctcc gccctgttca aagccaaacc
     1681 caaatgggtg atggcggcag aactcgttga aacgacaaaa ctttatgcgc gcgacgtcgc
     1741 cgttatccag cccgaatgga tagagcagga agccccgcac ctcgtccgct accactattt
     1801 cgagccgcac tgggaacaaa aacgcggcga agtcgtcgcc agcgaacgcg tgacgcttta
     1861 cggtctgacc gtattgccgc gccgccccgt gtcttacggc aaagttgccc ccgaagaagc
     1921 gcgcgaaatc tttatccgca gcgcgttggt ggcgcaggaa tgcgatttga aagcggattt
     1981 ttttgtccac aacaaaaagc tgattaaaga aattaccgaa ctcgaacaca aatcgcgcaa
     2041 gcaagacgtg ctggtcgatg acgaagccct gtttgcgttt tataacgaac gactgcccga
     2101 aatggcttgg aaagacgcgc aaggcagcgt ttggggaagt gaagattccg tacggattat
     2161 tgaatctgac aaagccgaga ggtcgtctga aaatgagcgc aacgagtttc gtaaaaacaa
     2221 gcgtaatggg tctcgccaaa atgaaaatca cggcaacacc gtaggttggg ttgaaaaccc
     2281 aacatcagcc gcaactgcaa aaactgttgg gtttgacaac ccaacctatg ctactcagca
     2341 acccaccccc tccctcgcgg gggagggtcg gggagagggc aaaacagttg ccgctcaaac
     2401 taacttttct gcaacttcag taagccctct ccctaaccct ctcccacagg agagggaaca
     2461 gagtgccgcc gcttcaacgg tttcagacgg cctgcatcct gcaaattccc agcaaaccgc
     2521 cccctccctc gtgggggagg gctggggaga gggcaaaaca gttgccgctc aaactaactt
     2581 ttctgcaact tcagcaagcc ctctccctaa ccctctccca caggagagga aacagagtgc
     2641 cgcagcttca aaagtttcag acgaccccaa acccaaaaag cggccggcat ccccaaaagg
     2701 ccgtctgaaa cctttgcccc ttgccgatat ccgcaccttt gaagcctggc tcaaaaccgc
     2761 cgagcgcgac aatccgcgtc tgctgttcct cagccgcgac gatctgatgc aacacgccgc
     2821 cgcgcacatt accgaagagc agttccccaa attttggcaa accgcagacg gcaagttcaa
     2881 actttcctac cgcttcgagc cgcaccatcc gctcgacggc gttaccctca ccctaccgct
     2941 gaccgtcctc aaccgcctgc acgcgccgtc gctcgaatgg ctggtgcccg gtatgttgcg
     3001 cgaaaaaatc cagctgctga ttaaagccct gccgaagcaa atccgccgca tttgcgtgcc
     3061 cgtacccgat ttcatcacca aattcctcga aagcaatccc gaccgccaag cggccatcat
     3121 tccccagttg gcgcacttta ttgccaaaag cgcaggcgat atgcggattc tcgagcaaat
     3181 cgaccaagac gcatggacgg cgcaagaatt gcccgaacac tgctatctga atctgcgcat
     3241 tatcgacgac ggaggacaag agcttgccgg cggccgcaaa ctgcatgaat tgcaacaaca
     3301 actcggccaa gccgccgccg ttaccttccg cgacaacacc caagaattcg agcgcgacaa
     3361 cgtgaccaca tgggacattg gcaccctgcc cgaatccatc aaattcgcac gcggcaaaca
     3421 acagctcacc ggctacctcg gtctgcaaaa agaaaaagac ggccgcatcg ccctgcgcct
     3481 gttcgacacc accgaagccg ccgagcaggc acaccgtcaa ggcgtgatcg aattgatgaa
     3541 actgcaatta aaagagcagg taaaagattt gaacaaaggc atccaaggct tcacccaagc
     3601 cgccatgctg ctcaaacaca tcaacgccga caccctgtgc gacgacctca cccaagccgt
     3661 ctgcgaccgc gcctttatcg gcgaagacga gctgccgcgc aacgaaaaag ccttcaaaga
     3721 acaaatcaaa cgcgcccgca gccgcctgcc cgccgtcaaa gaagccctca gccgctactt
     3781 gcaggaaacc gccgccgcct acgctgaact caacggcaaa ctcggcaaac acccgctcac
     3841 ccatctccta aaactacgcc tgcaaaccct gctcgccgcc ggcttcgcca cccgcacccc
     3901 gtgggcacaa tggccgcgcc tccccatcta cctcaaagcc atgaccctgc gcctcgaaaa
     3961 atacagcagc aaccccgccc gcgacgcagc ccgcgaagcc gatatccaag agctggaaca
     4021 aatgtggcag gaaaaaacag acagcctgat taaacaaggt ctccccattt cagacggcct
     4081 cgccgcgttt aaatggatga ttgaagaatt gagggtgtcg ctgttcgcgc aggaattgaa
     4141 gacaccgtat ccggtgtcgg tgaagcggtt gttgaaggag tgggagaggt tgaataagta
     4201 gtgatattat ccaatacatt tacaggaaaa tttctaactt acataaaagg tacaactcaa
     4261 tgaaaagcga aaataattat gaacgtattg ttcaaaattc taaaaaattc caacactctc
     4321 agaagaatga gatgcagaaa cgcataacag atttgctgca acaactcaca aatggcattc
     4381 atgaacgcga ccaaattatg gcggtttccc tacttggtgc gattgccgga cataatactt
     4441 tcctatttgg accgccggga accgcgaaaa gtctgatttc ccgccgtttg gcatgtgcat
     4501 ttgaaaatcc gcaatacttt gaatacttga tgaaccgttt cagcacacct gaagaagtat
     4561 tcggacctgt atctattaag gcattgaaag aagaccgata tctccgaaaa atcgaaggct
     4621 atttgccaac tgccgatttt gcatttttgg atgaaatttg gaaatccagt cctgcaattt
     4681 tgaataacct gctgaccatt atcaacgagc atatttttaa aaacggcgat gagaggttta
     4741 atgtgccact taaagccctg attgcggctt ccaacgaaat tccacctgaa aatcaaggtt
     4801 tagaggcact gtatgaccgc tttattatcc gtctgcttgt gccgccgatt gaacaagaag
     4861 agaacttcaa ccgtttgctg gatagcaagc caagcagcga caaacctgaa atcagtgaag
     4921 atttacggat tagttatcat gagcttgcca aatggcgtga acaactccac aaagtcaggc
     4981 taagtacaga tacattactg attattaagt atatcagaga agaattagca gcccagtttg
     5041 ataagttagg cgtttatgta tccgaccgac gttggcaacg tgcagccgta ttattaaagg
     5101 cttctgcttt ttgcaatgga cgcaaagaaa ccaatcatag cgatgtgatc ttgctgaaat
     5161 attgtctgtg gacaacacct gaaaatcgag agtcagtgga acaaattgtg atgaatacca
     5221 ttaaatcctg tgggtttgat acaggtattg atctggctga attagataga gaaaaagaga
     5281 gtttggataa ggaaataaat aaggaacttt atcattcaca agatgtttat gagacggttg
     5341 aaatgagtgg aaaaaaatat tttccagtac aggctgtttt caaaaacgta cataatagtc
     5401 atgctaataa agaacttaag atttatgttc catatagcaa atttcgtagt aaagaaaaat
     5461 tttatccaat agatagtaca ggaaatgaaa ttcaagaaat tgaatgtgaa tttgatgggc
     5521 aaggttgctg taaattggaa ttgaaagtgc ggggtggtca ctggaatgag caatataagc
     5581 cattcaaatt taaacctaac gttttattcc ataagggaga taaaaaacaa agcatcaatg
     5641 ataggttgat ttggtcttta tcacgttctg ttatggaaat caataaagca ttattagatg
     5701 tattgaaatt agtcaccaaa aaacatgcag aacatcagca attcctacaa agccctttta
     5761 ttacccaaga tgatattgat attgcggtat caggaatttt agaccaaatc gacaaactca
     5821 atttacggat taaagactgc gaacgtttgg agtctttatg tcgttaaata gcaaattatc
     5881 cgaacaaata agatggatta aagcttctct acaaatagat tatgcagatt tatttaaaca
     5941 gcatggttcg ttaaatggac agattcagca acgtttatca gattggcaat atcagaccaa
     6001 actggctttg ttgcagaaaa acccgtttgt attacatgaa gaaaagctaa aaaaacacca
     6061 acaaacagaa atttttaatc aacagtctgt tgatgattat cggtattttt gcaaattagc
     6121 aggcgtgcca tttgaaaaag aattctggca gcaggaatta acacaaagca aaaattcaaa
     6181 acccgataaa cagaatttgc ctttgaaatt gctaacagaa aaatggcagc agcaattaga
     6241 ctatgccaaa tctcaatggt atttagagca gcttaatcaa ttacgacagg aatttataga
     6301 taagttaaaa caggaactcg acactatccg acagcttgca gaacagttag agcaatttgg
     6361 ttttgaagca gggctgtggt ttgataacag tatcggcaat ttaagccagc aaaatattga
     6421 agaaatgaaa cgctggctga attacttgac ccaagataag aatgcccaac agattgctga
     6481 attactaggc aaaatgcgcc aaatcgaaca atctgaaaaa atcgaacagg taaaacagac
     6541 tgtttacaca aaaagcccac aaattgatgt gaattcgcgt gaagaaatta tcggattacg
     6601 tttaggtaaa gatttagagt atgtattgcc gtctgaattg gctttaatgg cagatgaaga
     6661 aacatcgact ttatttgatt tgaagttttt ggaatccaaa ttaatgtgtt ttgaattgca
     6721 aggaatgacc tattgcgatg ccccaactga aattatagtt gaacaaaaaa gccaagagga
     6781 tgaaaagctc ggaccaatga ttttatgtgt agataccagt ggttcaatgg ttggtctacc
     6841 tgaaaatatt gccaaagcaa tggctttatt tttaggagca aaagctaaaa gcgaaaaccg
     6901 ttcttgtttt gttatcaatt tttccactgg aatagaaact tttgaattaa caagtaaaac
     6961 agggatttcc aatttgattg cttttttacg tcaatccttt catggtggta cagatgctgc
     7021 cccagcttta cgtcatgcgc taaaaatgat ggagcaagaa atctatcaaa aggcagactt
     7081 attaatgatt tccgattttg tgatgaacag cttacctgat gatttgttgg catcaatcga
     7141 aatacaacga gaaaccggta atcagtttaa ttctttggtt attggtgatg cttttatgag
     7201 taaacgccta aaaacacatt ttgatcgtga gtggatatac aatcctaatg ttcagacaat
     7261 tcaa
//
LOCUS       DAWFBO010000099         7245 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFBO010000099 DAWFBO010000000
VERSION     DAWFBO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.099"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(47..802)
                     /locus_tag="WGO87_001452"
     CDS             complement(47..802)
                     /locus_tag="WGO87_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0392804.1"
                     /translation="MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPRAGE
                     LKQPVTNVSWFAANAYCTAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(980..2158)
                     /gene="nirK"
                     /locus_tag="WGO87_001453"
     CDS             complement(980..2158)
                     /gene="nirK"
                     /locus_tag="WGO87_001453"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ0392805.1"
                     /translation="MKRQALAAIIASMFALAACGGEQAAQAPAETPAASAEAASSAAQ
                     AAAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYHYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGSIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            2540..4795
                     /locus_tag="WGO87_001454"
     CDS             2540..4795
                     /locus_tag="WGO87_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0392806.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSKEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEIPAEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IVISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            4974..5507
                     /locus_tag="WGO87_001455"
     CDS             4974..5507
                     /locus_tag="WGO87_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0392807.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRDKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5570..6754)
                     /locus_tag="WGO87_001456"
     CDS             complement(5570..6754)
                     /locus_tag="WGO87_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0392808.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDTQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILGKQRAIVFGNFRMGNIRDVYDSSYDFSTVVNHISRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNGSYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6794..7198)
                     /locus_tag="WGO87_001457"
     CDS             complement(6794..7198)
                     /locus_tag="WGO87_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0392809.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPKLRVITVHNDSGSPAFCRVIWHE
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1605 a         1770 c         2033 g         1837 t
ORIGIN      
        1 aggcaacgcc gtactggttt ttgttaatcc actataattg aaggggttat cggcttgtgc
       61 aacggaagcc caagttgtgc aagacatatt tggattgcag gctggtacgg atgccgtagc
      121 ggaggaaggc ggcatagttg gacgagtcgc tcgacccgat agacgcgccg ctgcaaaaca
      181 tttgcgcgtt ggcattgccg gaagaaagca ggctgctgtt gaaatcttcc gtccattccc
      241 aaatcagccc gtgcatatca taaacgcccc agtagttcgg gcggcctttg ccgacatcgt
      301 gcaggccttt ccgtccgccg tcggcatacc aatcgagaat agtgcggttg tagccgggtt
      361 cgtttgagcc gtttttctgc gtggcggaag caagtccggc aaattcccat tcgtcaatgg
      421 tcggcaggcg tttgccttgt gcggtgcaat aggcgttggc ggcaaaccag gaaacattgg
      481 ttaccggctg ttttaattcg cctgccctgg gtgcgtagct gcggctgccg tttttcatcc
      541 aatgcttcag ataagcgggt tctgcctgtt tggaaccgat cctgcctttt tgccattggg
      601 ggtggctgtt gacaaattcg gcaaactcgg cattggtaac gggatattta tccagtttga
      661 acggtttgac tttaatcagg ccggtatctt ttttcagata aagcgggcgg tagctgccgc
      721 cttcgatttg aaccatttcg gcagccgccg cttgagtgcc ggcgagtgcc gcaccgagga
      781 aaaataaccg gacgtacttc ataaagcctc ctgacaggcg gttagcattg atttttcaga
      841 cggcatttta aacgaaaagc tgcgaatgcc gtctgaagaa tcaatcttcc gaaaggaaag
      901 attggttgtt aaaaaaccac tgccgtgcgt aatgaagtac ggcggcagtg gtttgtcccg
      961 ctaatgacgg gtatccggtt taataaacgc ttttttcgga tgcagaggct gccggggcag
     1021 aagcagccgg tgcggaagca gcaggggcag aagccgcgcc gctgccggcg taagcggtat
     1081 cactcaattt ttgagtcata atttcagggt tttccgcacc ctctactttc aattggccca
     1141 acgcgccttt gttgaatgcg cggaagatgg agtggtcgac caaagtgtag ctgcccggga
     1201 tgtcgacttt gaattcgaca atggcggagc ctccggcagg aacgatggtg ctttgtacgt
     1261 tttcgttaat cagcttgccg ccttcaacat aaactttgtc gaagatttcg ccgatgacgt
     1321 ggaaggaaga caccaggttc ggaccgccgt taccgacgta catacgcacg gtttcgcctg
     1381 ctttggcttt cagcgcatta tcgccggcga tagaacctac atgaccgttg aatacgacgt
     1441 attcaggctg ttcggcaacg gctttgtcca tgtcgaacgg ttgcaggcct tgcgcgcctt
     1501 ttttgccttt ggtgtagaag tcgccttgaa cgatgtagaa ctctttatcc actttcggca
     1561 ggccttcttt aggctcgacc aaaatcagac cgtacatacc gttggcgatg tgcataccga
     1621 ccggcgcgac ggcgcagtgg tagatgtaca ggcccggttg cagggctttg aagctgaatg
     1681 tggaagtacg gcccggggcg gtaaaggtcg cggctgcacc gccgccctga ccggttgcgg
     1741 cgtggaagtc gacgttgtgc ggaacggtag aagacgggtt gttggagaat tcgacttcta
     1801 cagtatcgcc ttcgcgtacg cggatcatgc ggcccggaac gtcgccgtca aatgtccagt
     1861 agtggtattc caccccgtcg tccattttca tggttttttc gacggtttcc attttcacgc
     1921 ggactttggc aggatagtcg cggtcgattg cgggaggtac ttcgggagcg tgggtggtaa
     1981 ccgcatcgat gacgggcagt tcgcctgcag gcgtttcggc ggcagcttgt gcggcggaac
     2041 ttgcggcttc tgcggaagcg gcaggggttt cggcaggagc ttgagcagct tgttcgccac
     2101 cgcaggcggc taaagcaaac atagaagcga taattgcagc taaggcttgg cgtttcatga
     2161 tgttttcctt ttgtaagaaa agtaaattgt cctgaaagga ttggttaaga acttggtgtg
     2221 attatgcggt tgccccattg cgggcatttt gatttaagtc aaataattca taaaatatga
     2281 acttaaaatg acacttctaa tactttataa ctactaaaaa ttacaatgaa taggctgatt
     2341 tgccagggct tcggcagagc aggcaaaggc agggcaattt ccgagtaaag aataatgata
     2401 gttattatca tatatgtatt tgttttatat gtgagtttgt ttgaacaaat gtttatttgc
     2461 gcggtaaacc gtgctacaat ctttaacatt catattctgt gaattttaaa caactgcttt
     2521 attaaaagga gctctcaaaa tgggacagta caagaagctg tggtacttgc tgtttgccgt
     2581 tctggcagta tgctttacca ttcttggcta tatgggcagc gaggtttata agaaagcccc
     2641 gccttacccc gaacaggtcg tttccgcatc aggcaaggtc ttgatgacga aagacgatat
     2701 tttggcaggt cagtctgcgt ggcagagtac cggcggtatg gaagtcggtt cgattctggg
     2761 ccacggcgca tatcaggctc cggactggac ggccgactgg ctgcatcgtg agttgtccgc
     2821 ttggttggat ttgaccgcgc aacagactta cggcaaaaaa ttcgacgagg tttcccccga
     2881 agaacaagcc gttctgaaaa cccgcctggc tgacgaatac cgcaaccaaa gccgcgtgaa
     2941 ggaagacggt tctgtggtca tcagtgatac gcgcgtgaaa gccatcgaaa gcatcctgcc
     3001 gtactaccac ggtgtttacg gcgacgatcc cgcactgcaa accacccgcg aacactttgc
     3061 aatgaaaaac aacacattgc caagcaaaga agcgcgtgaa aaactgttcg acttcttctt
     3121 ctggacttct tggtctgctt cgaccaaccg tcccggcgag gtctttacct acaccaacaa
     3181 ctggccgcat gagcctttaa tcaacaacgt accgactact gaaaactata tgtggtcgtt
     3241 taccagcgtt gtattgctct tgatgggcat cggtttgttg atgtggggtt attccttcct
     3301 gaccaaacac gaggaagtgg aaatcccggc tgaagatccg atttccaaaa tccaactgac
     3361 cccttcgcaa aaagcattgg gcaaatacgt cttcctgacg gttgccctgt ttgtggtaca
     3421 agtattgctg ggcggcctga ccgcgcacta caccgtcgaa ggtcagggtt tctacggcat
     3481 cgatgaggcg ttgggctttg aaatgtccga ctggtttccc tacgccctga cccgtacttg
     3541 gcacatccaa tccgccatct tctggattgc aaccggcttt ttgacggcag gtttgttcct
     3601 cgcgccgatt gtcaacggcg gtaaagatcc caagttccaa cgtgccggcg taaacttcct
     3661 ctatatcgcc ctcttcatcg tggtcggcgg ttcttacgcg ggcaacttct tcgcgttgac
     3721 gcacatcctt cccccggaat ttaacttctg gttcggacac caaggttacg aatacctcga
     3781 tttgggacgt ttctggcaac tccttttgat ggtcggactg ctgttatggc tgttcctgat
     3841 gttgcgttgc acggtttccg cctttaaaga aaaaggcgtg gacaaaaacc tgttggcaat
     3901 ctttgtcgct tctatggtcg gcgtgggcgt gttctacgcg ccgggcctgt tctatggtga
     3961 aaaatccccg attgccgtta tggaatactg gcgttggtgg gtggttcacc tgtgggtgga
     4021 aggcttcttt gaagtatttg ccactgccgc ctttgccttt gtcttctaca atatgggctt
     4081 tgtccgccgc agtaccgcca ctgcctccac tttggccgct gccgccatct ttatgttggg
     4141 cggcgttccg ggcacgctgc accacctgta cttctccggc tctacctccg cctctatggc
     4201 aatcggcgcc tgcttctccg ctttggaagt cgtgccgctg gtgttgctgg gccgtgaagc
     4261 atacgagcat tggtcttacc aacacctgtc cgactgggca aaacgcctgc gttggccgct
     4321 gatgtgcttc gtggcagtcg ccttttggaa tatgatcggt gccggcgtat tcggcttcct
     4381 gatcaatccg ccgatttccc tgttctatat tcaaggtttg aacacttctg ccgttcacgc
     4441 gcacgccgcc ttgttcggtg tgtacggttt cttggcattg ggcttcgtat tgttggttgc
     4501 ccgttatttg aaaccgaacg cgcagtttga tgacaaactg atgacttggg gcttctggct
     4561 gctcaacggc ggcttggtcg gtatgattgt tatcagcctg ctgcctgtcg gcgcgattca
     4621 ggcatacgcc tccatcacgc acggtctgtg gtatgcccgc agcgaagagt tcctgcaaat
     4681 ggaaatcctc gataccttgc gttgggtacg tacggcagct gacttgatct ttatcggcgg
     4741 tgcaatctgc gttgccatcc aagccactaa aatcgtattc ggccgcgaca aataagtttt
     4801 gcggtatgaa atgagcaact gcacccgttt tcgggtgcag ttttttattg cggattggga
     4861 cgggaatgga aaaggatgtg gattttttat tttaatcggc tatacaattg ctgctttcat
     4921 gccgtctgaa gcacgtttca gatggcatcg gtttacatca gataaggata aaaatgggta
     4981 tttacgattt tcaaatgaaa gatgcagaag gcaatgcggt tgatttgtca ggctatcgcg
     5041 acaaggttct gctgattgtc aacacggcaa cgcgttgcgg tctgaccccg caatacgagg
     5101 ctttgcagaa gctgtatgca caatataccg cagaaggctt ggagattttg gattttccgt
     5161 gcaaccagtt ccgcgaacag gctcccgaaa gcagcggaga aattgcccaa gtgtgtatga
     5221 tgaagtttgg cacgaagttt aagattttcg acaaaatcga agtcaatgga gcaaataccg
     5281 cgcccttgta tgcctacctg aaatccgtca aaccgcaaga taaaggcaat catctgttta
     5341 aagatttcgt gttgaagctc gcagctttgg gagagaagcg cgacgaaggc gacatcaaat
     5401 ggaactttac caaattcctc gtcaaccgcg acggcgaagt ggtcgagcgt tttgcgccta
     5461 gtgttacgcc cgaagaaatc gaagccgata ttcgggcatt gctgtaatct gccgccgtct
     5521 tgggaaatgc cgtctgaagg cttcagacgg catttgtccg tttgcgggtt tattccgaga
     5581 tatcggcaac accgctttcg gggaagatgg gcaaatccgg cggtgggagc aaggtatcca
     5641 aagtcagggc ggacgcatcg agtgtggggt agccttcaaa cgcgaccgaa taactgccgt
     5701 ttccgttcat acggattcgg gcgtgcgcgc ccagagggaa agtaattttg tcggcaatat
     5761 gtccgaacgg gaaacctgtc agcacaggaa tttttgccgt acgagaaata tggttgacca
     5821 cggtagaaaa atcataagac gaatcgtata catcgcggat attgcccata cggaaattgc
     5881 cgaacacgat ggcgcgttgt ttgcccaaaa tacccgaaag atacagcgta ttgagcatac
     5941 gttcgatgcg gtagggctgt tcgccgacat cttcgaggaa caaaatgccg ccgtcgatgt
     6001 cgggcatata aggcgtgccg gcgagcgagg cgaggacgct taagttgccg ccccacaata
     6061 tgccttcggt ttcgacatcg gcgcgttgga tataaggaac atcaacggtc aggcggtttt
     6121 gggttgcacc cttgataaag gcatccatcg taaacgcacc gggggcgggt ttgccaaaat
     6181 cgctataagc catcgggccg gcaaaactca tcatattgcc ttttgccaac aatgccagct
     6241 ggacggcgca tacgtcgctg aatccgaaaa acagcgtgcc gtgttcgcgc atccttgcgc
     6301 cgagcgaagc aaaatcgata tgcggcagaa tccgcgccgc accgtaaccg ccgcgcaagc
     6361 ccatcagcac tttaggcgtg gcgacgcggc cggaagcgac ctcttggaaa tcggcggcac
     6421 gttgcgtgtc cgtgccggca aaccgttgga aacggcggct gcccgcctgt tggttggtta
     6481 cggtaaaacc gacattgtaa aggcgggtta aggctgtgtt gacgcggttg gtgtcttcgg
     6541 caaatcctga cgaagcgaca acgcgcagaa ggttgccgtc cgaactttga cggcgtggcg
     6601 tttggagagg cacggttcgg gctttcacaa cggaatggga agagggaagg ggcggaacgg
     6661 atgtggcgga tgtgccgcaa gcctgaagca gccccgcgcc ggcagcggcg gtgcaggttt
     6721 tcagaaaacg gcggcgggag gtcggttcgg tcataatttc cctttcatac ggtttcagac
     6781 ggcattggcg ggcttaaagc agctttttaa gctgttccgt ccaatcttgc ggcagggtca
     6841 taccgtgttt ccggacgggg tttgcccaaa tcggcgcggg gaaagacgca tcgttttcaa
     6901 agcgggcgat aatatgccaa tgcaggtgcg gcacgacatt gcccaagctg gcgaggttga
     6961 tttttgccgg ccggaacact tggcgcatag cggcttcgac tttgtacacc atttccatca
     7021 attcgccgcg ttccgctgcc gaaaggtcgg tcatttcggc gatgtgttca tgccagataa
     7081 cacggcagaa tgcaggcgaa ccgctgtcgt tatggacggt gatgacgcgc agcttggggg
     7141 tttgcagcaa aacgtcttca ttttgcgccg tgcagatggg gcagggttgg gcggtcattt
     7201 ttccttattc ctgaaagtgt ttaaaaaacg gtaggataat ttctg
//
LOCUS       DAWFBO010000100         7230 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFBO010000100 DAWFBO010000000
VERSION     DAWFBO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.100"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(36..521)
                     /gene="purE"
                     /locus_tag="WGO87_001458"
     CDS             complement(36..521)
                     /gene="purE"
                     /locus_tag="WGO87_001458"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0392810.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEACVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(561..1277)
                     /locus_tag="WGO87_001459"
     CDS             complement(561..1277)
                     /locus_tag="WGO87_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0392811.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGRIVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFAAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRVRMGNCESVIEIGRRYANRFKGTPAAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQTYPGSPAAKRAAAAVRKR"
     gene            complement(1277..1945)
                     /locus_tag="WGO87_001460"
     CDS             complement(1277..1945)
                     /locus_tag="WGO87_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0392812.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPMPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(1956..2141)
                     /locus_tag="WGO87_001461"
     CDS             complement(1956..2141)
                     /locus_tag="WGO87_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0392813.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            2284..4260
                     /gene="mutL"
                     /locus_tag="WGO87_001462"
     CDS             2284..4260
                     /gene="mutL"
                     /locus_tag="WGO87_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0392814.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELDGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSDNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFNRASDYPTGNKPDTRNAFGASGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDADLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4355..6484
                     /gene="dnaX"
                     /locus_tag="WGO87_001463"
     CDS             4355..6484
                     /gene="dnaX"
                     /locus_tag="WGO87_001463"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0392815.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAATSCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPETETAQTSVQTASAAAMPSESKTAEPVSNQENNDVPPWEDAPDEAQTAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSETPSENPIQATPNNEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWQDEAGRETPAMQDKRVQIEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5544..5583
                     /gene="dnaX"
                     /locus_tag="WGO87_001463"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6565..6900
                     /locus_tag="WGO87_001464"
     CDS             6565..6900
                     /locus_tag="WGO87_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0392816.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGL
                     PPGVGDFFR"
BASE COUNT         1786 a         2216 c         1830 g         1398 t
ORIGIN      
        1 aatttaagcc actatatatt cgcggacggc gggttttaaa tctgttccaa ttccatactc
       61 aaaacagcct gttcctgttt ggctcggaag tctgccagtt tttgcgccag ttcgggggtt
      121 tcgttggcga gcatggaaac ggcgaacaat gcggcatttg ccgcgcctgc ctcgccgatg
      181 gcgaatgtgg cgacgggtac gcctttgggc atttgtacaa tcgataaaag cgaatcttcg
      241 ccgcgcaggt atttgctggg gacgggtacg cccaaaacgg ggacggtggt cttggcggca
      301 accataccgg gtaaatgcgc cgcgccgccc gcacccgcga tgatggcttt gatgccgcgc
      361 gcccgtgcgg tttcggcgta ttggaacatt aaatccgggg tgcggtgtgc ggaaacgacg
      421 cacgcctcat attctacgcc gaactcttca agaaactgcg ctgcctgccg cataacgggc
      481 caatcgctgt tgctgcccat gatgatgccg atttgaatca taaatcctcc ttggtgcgga
      541 tggggtaaaa agcggaaaaa ctatcgtttg cgtacggctg ctgcggcgcg ttttgccgcc
      601 gggctgccgg gataggtctg tatcaggctg cgccaagtcg cccttgcaat gtctttttgc
      661 tgcaacctgt attggcattc gccgattttg aatatggctt cgggtgcggc aggggtgcct
      721 ttgaaacggt tggcgtaacg ccgcccgatt tcgatgacgg attcgcagtt ccccatacgc
      781 accctgcttt gcagcaacag gtacatactg cgttgcgcga tgctgccgcc gtcgcctccg
      841 tccgcgcctt tcaacagggc ggctgcagcg gcgaacctgc cgcttttata gtgtttgagt
      901 gcctgattgt agaggttttg tgcggtttcg acggtatgtg cggatgcgct gccgccttcg
      961 gtattgaggt aatgctcttt caacttgcgg tcgtcgagtt tttggacgta tgccctgctg
     1021 gaagggtgtg ttttcgcatg ctccagtgct ttgactttgc cgtttaaggt ttccacttcg
     1081 ttcgacagcc ggacgattct gccttccaga tagtccaaac ggtcttgcaa ggtcggaacg
     1141 ggatagggaa tagcgtccga actacctgcc tctgcctgcg gttcgagccg catatcctga
     1201 atattgcggg aaacagggga agaacaggcg gcggatacgg acagccaaat gataaaaagc
     1261 ggtaatttgg tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg
     1321 tgatggggac aatccgcgta tcgttcggca ggttttgatt aaaatctttg aggatgccga
     1381 cgctgggcgg cgcatcggaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt
     1441 cgatggcaat gatgccgcct tgccggacaa gcttgaggca acgctcgaaa tattgcggca
     1501 tgggcggttt gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa
     1561 tcaaatcatc caatgtcagc aatgcgggtt gcaggtgcag gctgatttta tgtgccacac
     1621 cggcctcgtt ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg
     1681 taatccgccc gtgttcgggc aacgccaatg caagcgcggt gctgctgtat ccggtaaata
     1741 cgccgatttc cagatatttt tccgcacgga tcagctttgc cagccaaacc aaaaccgccg
     1801 cctgttcgcg cgcaatcgcc attttgccca tacggtgatg cccggtcttc tcacgcagcc
     1861 gcgtcaaaac gggatgttcg ggttcgccga tggcgttcaa atagttttgc aggtccggtg
     1921 caacattgga cagatgggtc gtcatttcgg cgggttcagt cttggtaata ggtataaggt
     1981 tttttcgcta cttttgccgc ctcgaagttt tcctgttctt cgggattgag ttcgacatcc
     2041 cacaaaagcc cccggttttc caaacgctgc tgttccaact caggtttttc ttcaatcagg
     2101 cggttgagga attgtgtggc ttcggattgg tagtgataca tctttgcgct ccaattttac
     2161 gggatacggc gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat
     2221 acaggcagaa agataagggg ttttattata gaataaagcg tttttgcaac ggaagcctac
     2281 cttatgccac gcattgccgc cctgcccgac catctcgtca accaaatcgc cgccggcgaa
     2341 gtagtcgaac gccctgccaa cgccttgaaa gaaatcgttg aaaacagtat cgatgcaggc
     2401 gcaacggcga ttgatgtcga gctggacggc ggcggcatcc gcctgattcg cgtcagcgac
     2461 aacggcagcg gtatccatcc ggacgacatc gaactcgcgc tccaccgcca cgccaccagc
     2521 aaaatcaaaa ccttaaatga cttggaacac gtcgccagca tgggctttcg cggcgaaggc
     2581 ttggcaagca tcgcctccgt cagccgcctg accctgacca gccgtcaaga agacagttcg
     2641 cacgcaaccc aagtcaaagc cgaagacggc aaactcagca gccccaccgc cgccgcccac
     2701 cccgtcggca ccaccatcga agccgccgaa ctcttcttca atacccctgc gcggcgcaag
     2761 ttcctcaagt ctgaagccac cgaatacgcc cactgcgcca ctatgctcga acgcctcgcg
     2821 ctggcgcatc cgcacattgc cttctcgctc aaacgcgacg gcaaacaagt gttcaaactc
     2881 cctgcacaaa gcctgcatga acggattgcc gccattgtcg gcgatgattt tcagacggca
     2941 tcattggaaa ttgacagcga caacggcgca ttgcggctct atggcgcgat tgccaaaccg
     3001 actttcgcca aaggtaaaac cgacaaacaa tactgcttcg tcaaccatcg ttttgtccgc
     3061 gacaaagtca tgctccatgc cgtcaagcag gcataccgcg acgtattgca caacgcactg
     3121 acgcctgctt tcgtcctctt tctcgacctg ccgcccgaag ccgtggatgt caacgtccac
     3181 ccgaccaaaa ccgaaatccg cttccgcgac agtcagcagg tgcaccaact tgtgttccac
     3241 acgctcaaca aagcccttgc cgacacacgc gccgacctga ccgaaagcgt cagcaacgca
     3301 ggcgaagtgt tgcatgacat taccggcgtt acgcctgcac caatgccgtc tgaaaacgac
     3361 agcgaaaatc tgtttaatcg cgcatccgac tacccgacag gcaacaaacc cgatacgcgc
     3421 aatgcctttg gtgcatcagg caaaaccgcg cctatgccct atcagtccgc atatgcgccg
     3481 caacaacgca gcctgtccct gcgcgaaagc cgcgcggcaa tgaacaccta tgccgaactc
     3541 tacaaaaaaa ccgacgatgc cgaccttgag ttaagccgat tcgagcaggc acgtttcggc
     3601 aatatgccgt ctgaaacgcc tgctccccaa acagatacgc cgctttcaga cggcatcccg
     3661 tccccatccg aactgccgcc gctcggtttc gccattgccc aattgctagg catctacatc
     3721 cttgcccaag ccgaagacag cctgctgctc atcgatatgc acgccgctgc cgaacgcgtc
     3781 aactacgaga aaatgaaacg ccaacggcag gaaaacggca agctgcaaag ccaacgcctg
     3841 cttattcccg taacctttgc cgcgtcccac gaagaatgcg ccgccctcgc cgattatgcc
     3901 gaaacgctgg caggcttcgg cttggaattg tccgatatgg gcggcaacac cctcgccgtc
     3961 cgcgccgttc ccgccatgct cggcaaagcc gatgtcgtct ccctcgccaa agacgtatta
     4021 aacgaactcg cccaagtcgg cagcagccaa accatcgagg aacacgaaaa ccgcatcctc
     4081 gccacgatgt cctgccacgg ctcgatccgc gccggccgcc ggcttaccct gcccgaaatg
     4141 aatgcgctcc tgcgcgatat ggaaaacacg ccgcgcagca accagtgcaa ccacggccgt
     4201 ccgacttggg tcaaactgac tttgaaagaa ttggacgcac tgttcttgcg cggacaataa
     4261 gccgaaagtg ctagaatacg ccgcccgaga ccgccgttca gacggcattc cgacgcaccg
     4321 acagaaacat cacgaccgaa accaagagaa aaatatggcc tatcaagttc tcgcccgaaa
     4381 atggcggccc aaaacctttt ccgacctagt cggtcaggaa cacgtcgtca aagccctgca
     4441 aaatgccttg gacgaaggca ggctgcacca cgcctacctg ctgaccggta cgcgcggcgt
     4501 gggcaaaacc accatcgccc gcatccttgc caaaagcctc aactgcgaaa acgcgcaaca
     4561 cggcgaacct tgcggcgtat gcgaaagctg tacgcagatc gatgccggac gctatgtcga
     4621 cctgctcgaa atcgacgccg cctccaacac aggcatcgac aacatccgcg aagtcttgga
     4681 aaacgcccaa tacgcgccga ccgccggcaa atacaaagtc tatatcatcg acgaagtgca
     4741 tatgctttcc aaaagcgcgt tcaacgctat gctcaaaacg ctggaagagc cgcccgaaca
     4801 cgtcaaattc atccttgcca cgaccgatcc gcacaaagtc cccgttaccg tattaagccg
     4861 ctgcctgcaa ttcgtcttac gcaatatgac cgcgcagcag gttgccgacc accttgccca
     4921 cgtcctcgac agcgaaaaaa tcgcctacgc cccccccgcc ctgcaacttt tgggacgcgc
     4981 cgccgccgga tcgatgcgcg atgccttgag cctgctcgac caagccatcg ccatgggttc
     5041 gggcagcgtt gccgaacaag acgtccgcca aatgatcggt gcggtcgata aacaatacct
     5101 gtacgaactg ctgacaggca tcgtcaacca agacggcgaa gccctgctgg caaaagcaca
     5161 ggaaatggct gcgtgtgccg tcggctttga caacgccttg ggcgaacttg ccatactgct
     5221 gcaacacctc gccctgatac aggcagtgcc gaatgccttg gcgcacgacg accccgattc
     5281 cgatattttg caccgcctcg cccaaaccat aagcggcgaa caaatccagc tttactacca
     5341 aatcgccgtc cacggcaaac gcgacctgag cctcgccccc gacgaatacg ccggctttat
     5401 gatgaccctg ctgcgtatgc tggcgttcgc acccttggcg gcaacatcgt gtgatgcaaa
     5461 cgccgtgatt gaaaataccg aacttcaatc cccatcggca caaaccgccg aaaaggaaac
     5521 cgccgcaaaa aagccccaac cgcgccctga aacggaaacc gcccaaacat ccgttcagac
     5581 ggcatccgca gcggcaatgc cgtctgaaag caaaactgcc gaacccgttt ccaatcaaga
     5641 aaacaacgat gttccgcctt gggaagacgc gccggacgaa gcccaaaccg cagccggcac
     5701 ggcgcaaacg tcggcaaaaa gcattcagac ggcatccgaa gccgaaacgc cgcccgaaaa
     5761 ccaagtttcc aagaacaagg cagccgacaa cgaaaccaat gcccccttgt ccgaaacgcc
     5821 gtctgaaaac cccattcagg caacaccgaa taatgaagcc gttgaaacgg aaacatttgc
     5881 acacgaagcc cctgcagaac ctttctacgg ttacggcttt ccggataatg actgccccgt
     5941 agaagacaac gcagaaatcc cgccgcccga ttgggaacac gccgcccctg ccgataccgc
     6001 aggcggaggc gcggacgaag aagcagaagc gggcggcatc ggcggaaaca acacgccgtc
     6061 cgccccgccg cccgaatttt ccaccgaaaa ctgggcagcc atcgtccggc atttcgcccg
     6121 caaactcggc gcggcgcaaa tgccggcgca acactccgcg tggacggaat accatcccga
     6181 caccggcctg atggttttgg cgatgaccgc cgaagcacgc gccaccgccg acaaaaaacg
     6241 cctcgacaaa atccgcgaca cccttgccca agcctacggg ctgcaactca ccctgcaaac
     6301 ccaagactgg caggacgaag ccggcagaga aacccccgca atgcaggaca agcgcgtcca
     6361 aatcgaagac aggcaaaaag cacaagcatt gctcgaagcc gaccccgccg cacaaaaaat
     6421 cctccaagca ttcggcgcgc aatggcagcc cgaatcactg gaattggcgg caaaccggcc
     6481 ataaacagat ataatgccgc ccgaaccctt cggacggcat tgccgtttcc ctcattcatt
     6541 caaacacaaa caggagtatt cagtatgttc ggaaaagccg gattaggcgg cctgatgaaa
     6601 caggcgcagc aaatgcagga aaatatgaaa aaagcgcaag ccaaacttgc cgaaaccgaa
     6661 atcgaaggcg aagcaggcaa cggcttggtc aaaatcacaa tgacctgcgc gcacgaagta
     6721 cgcaaaatcg acatcagccc cgatttgatt caagaagccg ccgacgacaa agaaatgctc
     6781 gaagacctca tcctcgccgc cctcaaatcc gcccgtgaca aagccgaaga aaccgcaaac
     6841 aaaacaatgg gcgcattcac gcaaggtcta ccccccggag tgggcgactt cttccgctga
     6901 tacccaaccg tcattcccac gcaggcggga atctagaacg tagaatctaa gaaaccgttt
     6961 tactcgataa atttccgtgc cgacgggtct agattcccgc gttcgcggga atgacggcat
     7021 attttttgca tttgatataa agggtcgttt gaattttgtt cagcaagtgc aaagtgttgc
     7081 acataaaagg gcgcaggata gaggcaaagc gggcgtaggt cgggctgtag caactgtatt
     7141 tttcaccccg tcgggcaaaa atatagtgga ttaacaaaaa ccagtacggc gttgcctcgc
     7201 cttagctcaa agagaacgat tctctaaggt
//
LOCUS       DAWFBO010000101         7201 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFBO010000101 DAWFBO010000000
VERSION     DAWFBO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.101"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(86..955)
                     /locus_tag="WGO87_001465"
     CDS             complement(86..955)
                     /locus_tag="WGO87_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0392817.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1050..1967)
                     /gene="hisB"
                     /locus_tag="WGO87_001466"
     CDS             complement(1050..1967)
                     /gene="hisB"
                     /locus_tag="WGO87_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0392818.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(1964..3061)
                     /locus_tag="WGO87_001467"
     CDS             complement(1964..3061)
                     /locus_tag="WGO87_001467"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0392819.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3107..4396)
                     /gene="hisD"
                     /locus_tag="WGO87_001468"
     CDS             complement(3107..4396)
                     /gene="hisD"
                     /locus_tag="WGO87_001468"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0392820.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4393..5292)
                     /locus_tag="WGO87_001469"
     CDS             complement(4393..5292)
                     /locus_tag="WGO87_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0392821.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5396..6049)
                     /locus_tag="WGO87_001470"
     CDS             complement(5396..6049)
                     /locus_tag="WGO87_001470"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0392822.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6128..6781)
                     /locus_tag="WGO87_001471"
     CDS             complement(6128..6781)
                     /locus_tag="WGO87_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0392823.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7058..7142
                     /locus_tag="WGO87_001472"
     tRNA            7058..7142
                     /locus_tag="WGO87_001472"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7092..7094,aa:Leu,seq:caa)
BASE COUNT         1476 a         1717 c         2125 g         1883 t
ORIGIN      
        1 tcataatcgg gactgatttt aagcgtgggg tgataaaaat gccgtctgaa atatgttcag
       61 acggcattgt gtttggaaaa ggcaatcagt gttctgccaa tttcaggtaa acgccggaaa
      121 cgtcctgttc gccgtagccg gcttcgactg ctttgcggta gctggcagca acggtttcga
      181 cggcgggcag ggtgttgcct gcctgttcaa gctctttgac ggcgaggttg aggtctttgg
      241 aggcgtgttt gagggcgaag gcgggtggga attcgcggtt tgcccacagg gattttttgg
      301 tttggaacat gggcgagtcc attgccgagc cgccgatggc ttcgacgatg gtgtcggtat
      361 cgatgccgaa ctgccgcgcc atcagcatgg cttcgctgta agcttcgccg aaaatgccca
      421 agagcgagtt caagacgagt ttcgcgcccg aacctttgcc gacatcgccg aaatggaagg
      481 tttttttgcc gacgagggaa aatatttttt gcagcgggtt taaaacggct tcgctgccgc
      541 cgaacagaat cagcagcgtg ccgttggtgg cgggcccgac cgatccggaa acgggtgctt
      601 cggcaaactg tccgcctgcg gcttcgacaa gtgctttgac ggcgaggttt tcggtcgggg
      661 agatggtgct catgttgacg atgattttgc cggccaatcc gtcgcggact ccgttcagga
      721 tgtcgcacac ggcggcatag tcggaaacca tcaggaaaat gacgggatag tcgcggacga
      781 gttcggcggt gttgccgtaa acttttgcgc ctttggcgga gatgggggca gttttgtcgg
      841 gcgagcggtt gtatacgccg acttcgatgc cgccgtccaa gagccgcgtt accataggca
      901 gacccatttg ccctaagcct atccagccga tttgtgtgta ttcgtttgcg gacatgatgt
      961 ttactccttt tctgtttgta gtgtagtgta atcttatctg ttccgatacc gtctgaaaag
     1021 cctgcgggtg tttcagacgg tatggttttt tatgcggtca gcgtgccttt ggtcgagggg
     1081 gtctgtcctg ccatgcgcgg gtcgtgttcg actgccatac gcagggcgcg cccgaaggct
     1141 ttgaatacgg tttccgcctg atggtgggcg tttttgccgc tgaggttgtc gatgtgcagg
     1201 gtcatcatac tgtggttgac gatgccgtgg aaaaattctt caaacaaatc gacatcgaaa
     1261 cgtccgatta gtgcgcgggt aaattcgatg ttgtacacga gtccggggcg gccggaaagg
     1321 tcgatgacga cgcggctgag ggcttcgtcg agcgggacgt aggaatgtcc gtaacggcgg
     1381 atgccttttt tgtcgccgag tgcctgccgg attgcttgtc cgagtgtgat gccgatgtct
     1441 tcggcggtgt ggtggtcgtc gatgtgcagg tcgcctttgc agctgatgtc gatgtcaatc
     1501 atgccgtggc gggcgatttg atcgatcatg tgttcgagga agggtacgcc ggtatccagc
     1561 ctgcttttgc cgctgccgtc gaggttgatg gagacggtga tttgggtttc gcaggtattg
     1621 cggttgacgg taacgctgcg gcagccggca gcagatgcgg tttcggcaat ttgggtttcg
     1681 gcagttccgg tgctttctgc ggcggtttcg gggacagtgc gttcgcggtg ttttctgtcg
     1741 agccagcctt tgggtttgcc ggtgtgtttt tcgagttttg ccatcaggct gggacggatg
     1801 ccgcgtgcat ctgggtcttc tgcctgcttt tcaaggcgtt gtttgagttt gtagagtgcg
     1861 acgggggtac ggtagccgca gagtttggcg agcttggaca gcgaacctgc ttcttgggcg
     1921 agggtcagaa agttgtgcag ttggcgttgt gttttagtca aattcatagg aaatccgttg
     1981 gttggcagta aagttggcga atgatgttga gaacggcatc gttttgtgcg gggctgccta
     2041 cggtaatgcg caggcagtgt tccaaaagcg ggtgcgcgcc atgcagtttt ttaaccaaga
     2101 tgcggttttg tttgagcgtg tcaaacaaca aatcggcatc gggtacgcgt atggtaatga
     2161 agtttgcctg acttggaaag gtgttcagac ggcatatttt gcccaattcg gcgaacatcc
     2221 gttcgcgttc gtttttcagg ctgtcgatgt tggcagagat gatgccgtag tgttgcaggg
     2281 cgagtttggc ggtggtcagg ctcagttggt tcatattgta gggcggcagg attttttgca
     2341 gttcgccgat gacttcggga cagcctgccg catagccgat acgcagtccg gcaaaaccga
     2401 ttttgctgac ggtacgcatg acgatgaggt tgggaatgct gcccgcctgc ggcaggaagc
     2461 tgtcgccgtt gaatgcgccg taggcttcgt cgacgacgac gatgccgtct gaagcttcga
     2521 tgacggcttc gatttcggca cgcgtgaaac atacgccggt ggggttgttg gggtaggcga
     2581 taaaggtcag ggcagggcgg tgcttcctga cggcttcgag gacggcgggc aggttgaggg
     2641 tgaaatctcc gttcagtgga acgccgacat aatccatgcc gtacagcgcg gcgttgtggc
     2701 ggtacatgac gaaactgggt tcggctgcca acattgccgc gcccggtttg gcggtcagca
     2761 tcgtgatgaa ctgtatcagc tcgtccgagc cgttgcccag cgcgatgtcg gcgcagtcgg
     2821 gaatgtcgaa cgccgaacgt aatgcttcct gtaaaccgct gccggaggga ttggggtaaa
     2881 gatggatggg cgcggcggca agccgtgcct gccattcctg caacagcgtt tcatgtccgg
     2941 caaaagggtg gacgggactt tccatcgaat cgagtttggc aaagccgggc ggaacgtcgg
     3001 caatctgata tgccgacata gcttgtatgt cgtcgcggat gaaggagcgg acggatttca
     3061 tcgtgtttcc ttaatggttg gaatatgcct gtacgccgtt tcggcattat ttcatacgga
     3121 actctgccgc gcgggcgtgg gcggtcaggc tttcgccgtg tgccagcacg ctggcggttt
     3181 cgcctaattt ttgcgcgccc tgttccgaaa cctgaatcag gctggagcgt ttttggaaat
     3241 catatgtccc caaaggcgag gaaaagcggg cggttcggct ggtgggcaat acatggtttg
     3301 gaccggcgca gtagtcgccg aggctttcgc cggtgtagcg tcccatgaaa atcgcaccgg
     3361 cgtggcggat ttttttcgcc cattcctgcg gattttcgac tgacagttcc aagtgttcgg
     3421 gggaaatgta gttggcgatt tcgcaggctt cgtccaagtc tttggcgagt atcatcgcgc
     3481 ccctgttgcc gagcgaggct tcgatgatgt cgcggcgcgg catagtttcg atcaggcggt
     3541 ccatagcggc ttctacttcg tcgagatacg cttgcgacgt gccgatgagg atggcttggg
     3601 caatttcgtc gtgttcggcc tggctgaaca aatccatcgc cacccaatcg gcaggtgtcg
     3661 tgccgtcggc gatgaccagg atttcagacg gccccgccac catgtcgatg ccgaccacgc
     3721 cgaacacgcg gcgtttggcg gcggcgacga aggcgttgcc cggaccggtg attttatcga
     3781 cttgggggat ggtttccgtg ccgtaggcga gggcggcaac cgcctgcgcg ccgccgacgg
     3841 tgaagacttt ggttacgccg gcgacgtatg cggcggcaag tacgatgtcg ttgcgttcgc
     3901 cttttggtgt cggcacgacc atgatgattt ctttcacacc tgcgacgtgg gcgggcatgg
     3961 cgttcatgat gacggaactc ggatacgccg ccttgccgcc ggggacgtaa atgccgacgc
     4021 ggtcaagcgg tgtgatttgt tgtcccaaca gcgtgccgtc ttcatcggtg tagctccacg
     4081 attccatttt ttggcgttgg tggtagcttt cgacacggcg ggcggcggtc tgcaatgccg
     4141 tctgaacgtc gttcggaatg cgctcgaacg ccgcgttcaa atcggcttgc gtgagtatta
     4201 aatcatcgat gcttttagcg tttgtctgat cgaatttgtt ggtgtattca atcaaagccg
     4261 catcgccgcg cttttgcacg tcggcgcaaa tgtcggcgac gatgcgttcg gtttcggggt
     4321 tttgcgcggt ttcaaaagcc agcagggctt tgagtccggc ttggaaatcg ggcgattggg
     4381 tgttgagttt tttcatgatt tggttttttc cttttttatt tttggattgg gggtcgtctg
     4441 aatattctct tgttcagttt ctgtctctgt tttgggtctg atatacgctt tttctccccc
     4501 gctaccaaac ttcaattcgg gcaggcattt ttgttgcaga ccgagtttgg ctaatacctc
     4561 ttcgctacga gaggtagtgc ggaaacggta acgggattgt tttctaccgt ttttggtttc
     4621 ctcaaccatt gtttcaaaac cgtccacttc gtatgcactg acaacagcgt tttgcagacc
     4681 ggtgtgaacg ccgataacgt atttcacttt tgaagcaaca tctttaccga taacccagtt
     4741 gcccaacgta cgcgatttga ggttggcatc gtcttggttg tcgaaaaggt agtctaattc
     4801 ttcgtcagta tctaaatcga aagcattgtg gattttgatg gcgagaatca gcccgtcggg
     4861 gttaatctcg ttaagtggga gagaagagaa tccaaagcgg cgatctagtt cttctacgct
     4921 aataccaccc ggaccatgcc cggcaatttt atttttgagt tttttaccca agacagattt
     4981 aacaaaatga attaaggcag attcggcggc gagtgcttcg acttcagtca gatgatagct
     5041 gatgatatag cgaccgagtt tcttgcattt ggagatggct ttgagtttcc gatcgataat
     5101 ctcgccggag actgggtctt gtgaacgact agcaacccat tcatgctcga agatacgatt
     5161 accgcagcct ttgccgatgt agaggatttt gtcttttttc aggtcggtca gacaatagac
     5221 ataaaaccga cgctcgccgt tattcaaaac ggaaagggtt gaatctgaga atttttttat
     5281 ttttgcaacc attttaactt ctcctataca gtttactgcc ggtgatattt tcagacgacc
     5341 tgtcggcggg aacggatagg gtcgtctgaa aacgcatctt tattcaaatg gatgcttact
     5401 tcgccttcac tgcgccgccg aacgcctgaa taatcggctc cagcagcgcg tatttcgttt
     5461 tcaaagcagc cttgttgacc accaggcggc tggaaatgtc gacgatgtgt tcgactgctt
     5521 ccaagccgtt tgccttcaag gtgttgcccg tcgaaaccaa gtccacaatc gcatcgctca
     5581 agccgaccag cggcgcaagt tccatcgagc cgtacagttt gataatgtcc acatggacac
     5641 ccttgccggc aaaatgagat gccgcgattt caggatactt cgtggcaatc ttcagacggc
     5701 atccgggttg cgaagctgct tcgtaatcaa accctttacg cacagcaacc atcatgcggc
     5761 acttggcaat ctccaaatcc aaaggccggt aaagccccgt gccgccgtgt tcgatcagca
     5821 cgtctttgcc cgcaatgccg aagtccgccg cgccgtagcg gacataagtc ggcacatcgg
     5881 ttgcgcggac aatgacaagg cggatgtttt catggttcgt cccgataatc agcttgcgcg
     5941 atttttcagg ctcttcagtc ggaacaatgc cggcagcggc aagcagcggc agcgtctcct
     6001 caaaaatgcg ccccttggat aaggcgatgg tcaaagcatt atcctgcatg gtgtggatgt
     6061 cctttgtcgg ttgaaacaat aatgccgtct gaataggcgc cgacggtgca gacggcatac
     6121 ggttttatca gagcagagtc cttacatcgg cagcaatcgt ttccggctcg cttccgtaag
     6181 gcgagaaaat ggcaacctca ccgtttttgt cgatgagata cgcaccggaa gagtggtcga
     6241 ccaaatagtt ttcgctgtcg tctttttgat tgactttggc agaaaccacg cggtattgct
     6301 gcttgatgac cggcaggttt tggtcgcccg ttgccgtcag accgataaag tccggattga
     6361 actgtttggc atacttgccg atgatttcag gcgtgtcgcg ttccggatcg atgctgacga
     6421 acaccacttt cacgtcctta gcctgcccgc ccaactgctt caacgtgtcg ctgtacgtca
     6481 aaagctctgt cgggcagaca tcgggacagt gcgtaaagcc gaaagacaga atcacgacct
     6541 tgcctttcaa atcgctcagg ctgaaaggct tgccttcgcc gtcggtcagc gtgaaatcgc
     6601 cgccgatgtc ttccttacgc atatccgtac cgcgcgtttg cggctttgcc gcattttccg
     6661 cagctgacgc ggatgcactt gaagaagcgg cttgcgccgc actgttgtct tgaggtttgc
     6721 aggcggcaag cgcggcaagt acgaaaacgc ccggcaaaaa ggaacgcggt acggaaaaca
     6781 tgacaatctc ccaagaataa atagcaaaac ctgattcgta cggaatttga atatttccat
     6841 cttaacgcgt ttggcagatt taatgtagta tttttttacg ttgtttgcac ggcaggaatt
     6901 gccgaatcat acagaatctt tggagcggaa agaacagtaa gattaaaata ataaaaattc
     6961 tatttaaaat caaaaataaa ataaaaacag ataaaataat ttcaaaaaaa aggtttacag
     7021 aatttcaaaa gccattataa tgacgacttc ttcaccagcc caggtggcga aattggtaga
     7081 cgcaggggac tcaaaatccc ccgccgcaag gtgtgtcggt tcgagtccga ccctgggcac
     7141 cacaaccgct tttagagcgg ttattttttt gtctattgct gtttaatgaa atagataaaa
     7201 c
//
LOCUS       DAWFBO010000102         7088 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFBO010000102 DAWFBO010000000
VERSION     DAWFBO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.102"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(69..428)
                     /gene="rnpB"
                     /locus_tag="WGO87_001473"
     ncRNA           complement(69..428)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGO87_001473"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WGO87_001474"
     CDS             complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WGO87_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0392824.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1713..2606)
                     /locus_tag="WGO87_001475"
     CDS             complement(1713..2606)
                     /locus_tag="WGO87_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0392825.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2948..3397
                     /locus_tag="WGO87_001476"
     CDS             2948..3397
                     /locus_tag="WGO87_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0392826.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3471..3779
                     /locus_tag="WGO87_001477"
     CDS             3471..3779
                     /locus_tag="WGO87_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392827.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3895..4737
                     /locus_tag="WGO87_001478"
     CDS             3895..4737
                     /locus_tag="WGO87_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0392828.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4727..5473
                     /locus_tag="WGO87_001479"
     CDS             4727..5473
                     /locus_tag="WGO87_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0392829.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5495..6082
                     /locus_tag="WGO87_001480"
     CDS             5495..6082
                     /locus_tag="WGO87_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0392830.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6088..6267
                     /locus_tag="WGO87_001481"
     CDS             6088..6267
                     /locus_tag="WGO87_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0392831.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6499..6939
                     /locus_tag="WGO87_001482"
     CDS             6499..6939
                     /locus_tag="WGO87_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392832.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1807 a         1884 c         1616 g         1781 t
ORIGIN      
        1 acacaaccta aataaaaatg ccgtctgaac catatttcag gtttcagacg gcatttgcgt
       61 atcggatgca caccggacgg gcggtaagcc gggttctgtc tcggacagtc attcctctag
      121 gcataccgtt accggtatgc tcaagcaacc tacccgaacg ctcggcgggc agcgtcattg
      181 cgttctgttt ggtcttgctc cgaatggggt ttggcctgcc gcatattgtt accaaatgcg
      241 cggtgcgccc ttaccgcacc ttttcaccct tgcctgtgct gccaaagcag ccatcggcgg
      301 ttttgctttc tgttccactt tccgtcgcgt taccgcgccc ggccgttaac cggcattcta
      361 ccctgcggag cccggacttt cctccccgta tgccttacgc gatacgcggc gactgtctgc
      421 tcgtcccgtg tgcggcgcgg attataacac gaaacgcaaa aatgccgtct gaaacggtac
      481 aggtttcaga cggcatacgg ttcaaactac acgccctgtt tcaggctggc ttcgataaag
      541 ccgtccaaat cgccatccaa tacggctttg gtgttgccga cttcgtagcc tgtacgcaag
      601 tctttgatac gtgaggaatc caaaacatac gaacggattt ggctacccca acctacatcg
      661 gatttacctt cttccaacgc ctgtttctct tcattgcgtt tgcgcatttc caattcatac
      721 agtttggact tcaacatttc catcgcggcg gctttgttgg cgtgttgcga acggtcgttt
      781 tgacattgca ccacaatccc cgtcggctcg tgggtaatgc gcacggcgga gtcggtttta
      841 ttgatgtgct gaccgcccgc gcccgatgcg cgataggtgt cgatacgcaa atcggcgggg
      901 ttgatttcga tttcaatgga atcatcgatt tcagggtaaa cgaacacgga ggcaaacgaa
      961 gtatggcgtt tgttgttcga gtcaaacggt gaatagcgca ccaagcggtg aacgccggtt
     1021 tcggtacgca gcaaaccata ggcgtattcg ccttccactc ggatagtcgc gcggttgatg
     1081 cccgcgattt cgccgtcgtc ttcttcgagg atttcgattt tgaagccttt gcgctcggcg
     1141 tagcggctgt acatacggaa cagcataccc gcccagtctt ccgcttccgt accgcccgcg
     1201 cctgcggtga tgtcgataaa gcagttgttc gggtcggcgg gctggttgaa catccgtttg
     1261 aactccaaat ccgccatctg tttttccagc cccgccacat cttcctgcac ggcggcaaaa
     1321 ccttcttcgt cgttttcttc gacggtcatt tcaatcagca tgcggttgtc ttcgatgccc
     1381 gaagcgatgt tgtcgagcgt caacacgatg ccttcgagga ttttgcgctc tttgccgatt
     1441 tcttgggcgc gtttcgggtc gttccaaagt tcggggtctt cggaaagacc gataacttct
     1501 tccaatcggt ctttcttacc ctgataatcc atataaactc ggatgtcttc gctgcgcttt
     1561 tccaaatcat tcagggtatt gttgagctgg ttgattactt cggcttccat gattcttttg
     1621 ttctttcaaa attttagggg cgtattgtac gggattcggg tatttttttc tatggataaa
     1681 gccttctaga aacacgttcg gacggcatag cgtcaataac ggtatgccgc cagtttgcgt
     1741 ttgatttcag gcaatgcggc acgtgctgcc tcctcaccca actggatggc acgttttttc
     1801 tgatcgaatc cgccgactgc acccaaatcc aaaacctgcg gtttgataac cacatccgcc
     1861 tgccccaact cattttgcaa cgcggaaacg ctcattacgt tcagcgtctg atcgagataa
     1921 gagaagaagc cttggctgat gtttttgccc ggacgggcgg aaatatcgac ggcaatcacg
     1981 aaattcgccc cctgccgccg ggcggcactg acgggcacgg gctgcgacag accgccgtca
     2041 acatatgtat gcctgccgat gataacgggt tggaacacat tgggaatggc ggcggaagcg
     2101 cgcacagcct gcccggcatt cccttgattg aaagcgacgg ccttgccggt ttcaaaatca
     2161 gtagcaacag cggcaaattt gatgggaaac tgctgaatct gcctgccgcc gacttttcgg
     2221 ttgatgtaat tttgcagctt ttcgcctttg ataaaaccac tggtggacaa ggttaaatcg
     2281 actaaatcgg ttttgcctaa aatttcggct tccaattcga ggcggtcggg cgacataccc
     2341 gatgcaaaaa ggctgccgac aatcgaacct gccgatgtgc cggtaaccac cttcacagga
     2401 ataccgtttt ctttcaaaac cttaataata cctacatggg caaatccttt agatgcgccg
     2461 ccaccgagtg ccaaaccgac cactgcggcg ggtttggcgg tttgcaccgg cttgcgggcg
     2521 gcattatttc ccgccgtgcc gcaggcggca agcaacgcgg cggcggcgat tgccaaaagc
     2581 ggtctgattt ttgaaaacgt taccatattt tccattcctt tatatatcgc accccgtcaa
     2641 aaagagggat tgcttttctt aacaaccccc tttgacagcc aagcaaatgg gggctttgtt
     2701 aagtcatcat caaaattaat atttcttttt ttcctttacg gaaattatat ttgaaggcat
     2761 actatccaag gtgggaatta tctcacaaca ccgccgttat ccaaatatcc cgcctttttc
     2821 ccttcctttc catcaaaata ctttcttttt atattcatta acttgttaaa tcattggctg
     2881 ccgggtgtca gtttttccga caaaatccgt ctaatggggt atcaacagaa ccaaaacagg
     2941 aacacttatg aaaatcggaa caacttggca gacggcatcc gctatgctgg ttttgcgtct
     3001 gtttgccgca tatgaatttt tggaatcggg tttgcaaaaa tggaacgggg agaattggtt
     3061 ttctgaaatc aacgatcaat tcccattccc gttcaacttg ctgccggacg cgttaaactg
     3121 gaatctcgct atgtatgcgg agcttttact gcccgtactg ttgcttttgg gtttggcaac
     3181 gcgtctgtcg gcattagggc tgatggtcgt taccgccgtc gcttgggctg cggttcacgc
     3241 cggttcgggt tacaatgtct gcgacaacgg ttataaaatg gctttaattt atatcgtggt
     3301 attaatcccg ctgcttttcc agggtgcggg cggatggtcg ctggatacgc tgctgaaaaa
     3361 acggttttgc ccccaatgcc gtctgaagca agattgattc agtcgtggaa tctgacttta
     3421 aacattccaa ccttatctcg ttaacttgat attctgaaaa ggaaatagaa atgaacaaaa
     3481 acattgctgc cgcactcgcc ggtgctttat ccctgtcttt ggccgccggt gcagttgctg
     3541 ccaacaaacc ggcaagcaac gcaacaggcg ttcataaatc cgcccatggc tcttgcggcg
     3601 cgtccaaatc tgccgaaggt tcgtgcggcg cggctggttc taaagcaggc gaaggcaaat
     3661 gcggcgaggg caaatgcggt gcgaccgtaa aaaaaaccca caaacacacc aaagcatcta
     3721 aagccaaggc caaatctgcc gaaggcaaat gcggcgaagg caaatgcggt tctaaataat
     3781 cccacccctt caaaccaagc cgcgtttttc agtaaaatgc ggctttttta acggcaaaca
     3841 aagatttttt aacaagcaca tcattctttt gtgccatccg aaccgggtaa aaatatgatt
     3901 caacacgcag gcttgggcta ccgccgcgac ttggcggaag actttctctc gctttccgaa
     3961 aacagcccga tatgctttat cgaagccgca ccggaaaact ggctgaaaat gggcggctgg
     4021 gcgcgcaaac agtttgaccg tgtggcggaa cggctgccgc tggcgttgca cggattgtct
     4081 atgtcgctgg gcgggcaagc accgctggat actgatttga tagacggcat caaagaaatg
     4141 atgcgccgtt acgattgcac gtttttctcc gaccatttga gctactgcca cgacggcggt
     4201 catctttacg atttgttgcc gctgcccttt accgaggaaa tggtgcatca tacggcgcgg
     4261 cgtatccgcg aagtgcaaga ccgtttgggc tgccgcatcg ccgtggaaaa cacgtcctac
     4321 tatctgcatt ccccgcttgc cgagatgaac gaggtcgagt tcctcaacgc cgtcgcacgt
     4381 gaggccgatt gcggcattca tctggatgtg aacaatatct acgtcaacgc cgtcaatcac
     4441 ggcctgttgt cgccggaggc ttttttgaaa aacgttgacg cagatcgcgt gtgctatatc
     4501 catattgccg gacatgacgt ggaaacgccg gaattgttga ttgatacaca tggcgcggca
     4561 gttttgccga ctgtttggga cttgctcgaa cttgcctatg ccaagctgcc gacgattccg
     4621 cccaccctgt tggaacgcga ttttaatttc ccgccttttg ccgaactcga agccgaagtc
     4681 gccaaaatcg ccgattatca aacgcgtgcc ggaaaggaat accgccgtgc agcctgaaac
     4741 ctccgcccaa taccagcacc gtttcgccca agccatacgc gggggcgaag ccgcagacgg
     4801 tctgccgcaa gaccgactga acgtctatat ccgcctgata cgcaacaata tccacagctt
     4861 tatcgaccgc tgctataccg aaacgcggca atactttgac agcgaagaat ggagccgtct
     4921 gaaagaaggt ttcgtccgcg acgcgcacgc ccaaacgccc tattttcaag aaatccccgg
     4981 cgagttccta caatattgcc aaagcctgcc gctttcagac ggcattttgg cactgatgga
     5041 ttttgaatat acccaattgc tggcagaagt tgctcaaatt ccggatattc ccgacattca
     5101 ttattcaaat gacagcaaat acacaccttc ccctgcggcc tttatccggc aatatcgata
     5161 tgatgttacc gatgatttgc atgaagcgga aacagccttg ttaatatggc gaaacgccga
     5221 agatgatgtg atgtaccaaa cattggacgg cttcgatatg atgctgctag aaataatggg
     5281 ttcctccgcg ctttcgtttg acaccctcgc ccaaaccctt gtcgaattta tgcctgagga
     5341 cgataattgg aaaaatattt tgcttgggaa atggtcaggc tggactgaac aaaggattat
     5401 catcccctcc ttgtccgcca tatccgaaaa tatggaaggc aattccccgg gccaaaacca
     5461 tctatccgca taaaattacc ttgttcccga tactatgccg ctacccgacc tgaccgatgc
     5521 cgaattaata gagtcgcgta aactgcttct gcattttgcg cggcttcagt tgcccgacca
     5581 ccctgatttg gctgaagatt tagtgcagga aacattgctg tccgcataca gcgcaggcga
     5641 cagttttcaa ggcagggcac ttgtcaacag ctggcttttt gccatattga aaaacaaaat
     5701 tattgacgca ttacgtcaaa tcggaaggca gaggaaagtc tttaccacac tggatgacga
     5761 gctactggat gaagcatttg aaagccattt ttcccaaaac gggcattgga cgccggaagg
     5821 gcagccgcaa cattggaaca ctccggaaaa atcattaaac aacaacgaat tccaaaaaat
     5881 tctgcaaagc tgcctataca acctgcctga aaacaccgca cgggtattta ccctgaagga
     5941 aatactcggt ttttcatccg acgaaataca acaaatgtgc ggtatcagca cgtccaacta
     6001 ccacaccatt atgcaccgcg cccgagaatc attgcgccaa tgcctgcaaa tcaaatggtt
     6061 caaccaagaa aacccgaagt aaacgttatg aaaaaatgcc gcgatatcgc cctgcttctt
     6121 tccaaacatc aggaccggga aaccaccccg ggcgagaaga tttccatata cacacacctg
     6181 ctgttctgtc cgtattgccg tgaatataaa agacaacttc aaaccatcaa aagatcactg
     6241 gcaaaaacaa ccagaacttc aaaataaatg ccgtctgaaa aggcttcaga cggcataagc
     6301 tgacggaaaa aaatcaaacc gatttactgt tatcggcagt tcatccataa tacacgcttc
     6361 aaaagcagca tattcctcca tacggaatat ataaatacgc aaaacacgaa ggctgcatca
     6421 atttgcttta tttgccttat ttcacagacg gcgctacccc tcccgcccaa cccgttcttt
     6481 ctgaatgagc agatttcaat gattaaggaa accctaatgc gcccaatctt cctatctttc
     6541 gttttactcc ctattttgat aaccgcctgc agcacaccgg acaagtctgc ccgatgggaa
     6601 aatatcggca caatctcaaa cggcaatatt catacatata tcaataaaga cagcgtgaga
     6661 aaaaacggaa atctgatgat tttccaagat aaaaaagttg ttaccaatct aaaacaagaa
     6721 cgttttgcca acacccccgc atacaagact gccattgccg agtgggaaat ccactgcaac
     6781 aacaaaacat accgcttaag ttcgctacag ttgtttgata caaaaaacac ggaaatttcc
     6841 acacaaaact acacagcctc ttccctccgc ccgatgagca tcctgtccgg gacattaacc
     6901 gaaaaacaat atgaaaccgt atgcggaaaa aaactctgat tgcaacttat acacaaactt
     6961 acccacaaac cttatcataa aaatgccgtc tgaaatactg aaatatcagc atttcagacg
     7021 gcattttgcc attccctgaa aattatccac aaagttatcc acattatttt ttaaaaccgg
     7081 cttccatc
//
LOCUS       DAWFBO010000103         7054 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFBO010000103 DAWFBO010000000
VERSION     DAWFBO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.103"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            140..721
                     /locus_tag="WGO87_001483"
     CDS             140..721
                     /locus_tag="WGO87_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0392833.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(783..1637)
                     /gene="folD"
                     /locus_tag="WGO87_001484"
     CDS             complement(783..1637)
                     /gene="folD"
                     /locus_tag="WGO87_001484"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0392834.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1860..1937
                     /locus_tag="WGO87_001485"
     tRNA            1860..1937
                     /locus_tag="WGO87_001485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1895..1897,aa:Pro,seq:tgg)
     gene            1962..2038
                     /locus_tag="WGO87_001486"
     tRNA            1962..2038
                     /locus_tag="WGO87_001486"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1996..1998,aa:Arg,seq:tct)
     gene            2066..2141
                     /locus_tag="WGO87_001487"
     tRNA            2066..2141
                     /locus_tag="WGO87_001487"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2099..2101,aa:His,seq:gtg)
     gene            2277..2783
                     /locus_tag="WGO87_001488"
     CDS             2277..2783
                     /locus_tag="WGO87_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0392835.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2780..4558
                     /locus_tag="WGO87_001489"
     CDS             2780..4558
                     /locus_tag="WGO87_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0392836.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4671..5495
                     /locus_tag="WGO87_001490"
     CDS             4671..5495
                     /locus_tag="WGO87_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0392837.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6257..7045)
                     /locus_tag="WGO87_001491"
     CDS             complement(6257..7045)
                     /locus_tag="WGO87_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0392838.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         1503 a         1826 c         2120 g         1605 t
ORIGIN      
        1 atgtatggcg taaaggaagc gttttaacgc cgtattggga aaatgtaaag ttctttatcg
       61 ggatttcttt aagtattggc gttttcggtg tcgtggcggt agaatcgggg tcagttaaga
      121 acaattatca ggaacggata tggaagaaaa aaacgactac accgacgcgg catccgacaa
      181 ccgcaacggg caggaaattg aagtcggaat tgccggcgca ggcgaccgga tacttgccgc
      241 gctgctgaac caattgttta cctttttggt tttgttggtg ccgtttgccg gactgattgc
      301 ttttgccatc aagaatgaag gcaggattgg cggcagtgag gaaatattcg ggctgctttt
      361 gggcatgact tccttttggg taggactggc gggcattttg gtttataccg tcgttcaaat
      421 ctactatatg agtcgggacg ggcagtcatt gggtaagaaa atcatgagaa tccgcgtgtt
      481 gaaaaccgac ggccgcaatc ccggttttgt cggcacggtt ttggtacgcg aaatcgcatg
      541 gtcggttttg gttgccatta ttgccgccgt tatcggtctt gcagtaggtg acaacggaga
      601 aaacgccatc aacctgctgg cattccttgc caactttgtc ctgctcttta tggtcaaacg
      661 cgaccgccgc acgctttacg acatactggc ggatacggtt gtcgtcaagc tgcccaaata
      721 aacggacacg gcaaaatgcc gtctgaaagc ttttcagacg gcatttttat cttcagaacc
      781 gctcaagcat cgtgcagtga agccgcgtgc agggtgtttt ccatcaatgt ggcaatcgtc
      841 atcggaccca cgccgccggg aacgggcgta atcatcgccg cccgttcttt tgccgtttca
      901 aattccacgt cgccgcacag gctgccgtcg tccaaacggt tgatgcccac atcgataacg
      961 accgcgccgg gtttgatcca ttcgcctttg acaaagttcg gaatgcccac gccgaccacc
     1021 aaaatatcgg ctccggcaac ctcgtctgcc agattttcgg ttgcgctgtg gcagaccgtt
     1081 accgttgcgc gcgccagcag caattccaac gcctgcgggc ggccgacgat attcgacgcg
     1141 ccgaccacga ccgctttttt ccccttcgga tcaatgccgt aagcttccaa aagcgtcatc
     1201 acgcccttgg gcgtacacgg gcgcatcagc ggcattttga ccgccagcct gccgacattg
     1261 taaggatgga agccgtccac gtccttatcc ggcgaaatac gttccaaaac cgcctggctg
     1321 tcgaggtgct tcggcagcgg tagctgaacc agaataccgt ccacttcgga atcggcattc
     1381 aggcggtcga ccagtgccag cagttcttcc tgcgatgttg attcgggcag ctcgtaagac
     1441 agtgatttga tgccgcattt ttggcaggca gttttcttgt tgcggacata aaccgcgctg
     1501 gcagggtcgc caccgaccaa aaccacggcc aggcaaggca tgtgcagatt gtcctgttgg
     1561 cgttgcgcta ccgcttcggc aaccgcctgc aggcgttttt gcgaaacttc tttaccattg
     1621 atcagttgtg ccgacatatc cgtccttttg ttaaacaggt agaaaatgct gaaaattatc
     1681 gcattttccg tttgttttta cagcaggatg ccgaaaataa atttgccctg aaataaaata
     1741 cggctgtttg gacggattaa atattttttc aaatcaaaaa actatatttt tcatcttggt
     1801 gtcttgaaaa taaatattga cgcatctttc cctgagttgt atagttcgct tcttcaagtc
     1861 ggggcgtagc gcagcccggt tagcgcatct gctttgggag cagagggtcg tgagttcgaa
     1921 tcccaccgcc ccgaccaatc aatcagcacg aatcggcatg agcgcccgta gctcaaccgg
     1981 atagagcacc gaccttctaa gtcgggggtt acaggttcga ttcctgtcgg gcgtgccaag
     2041 aatttgattg aaaaccgttt ttatggtggc tgtagctcag ttggtagagc cccggattgt
     2101 gattccggtt gtcgtgggtt cgagccccat cagccacccc agataaaggc ccgtactttt
     2161 cggtacgggt cttttctcgt ttgcgcgtac tgtccgcgcc gccggctttg cctttcgcat
     2221 atgcttctgt atcatgtggg tttttctgtc ttttatggtt tttgtcggag gctttgatgg
     2281 ttgacgcttg gtctgtccct gattttgaat cgaaaatctg tccgcccgag gcgttggcgg
     2341 cgcgtttggc gttgttgccg cgcccgctgg tgtttaccaa cggctgtttc gacatcctcc
     2401 acagggggca cgttacttat ctggcgcagg cgcgttcgat ggggggcgcg ttggtgctgg
     2461 cgttgaatac cgatgcttcg gtgcggcgtt tgggcaaggg cggcgaccgc ccgattaatc
     2521 ctttggagaa ccgtgccgcc gttgccgccg ctttggaaag tgtggatttg gtaacgtggt
     2581 ttgacgggga tacgccggcg gcgttgattg aggcggtcaa acctgagatt ttggtcaagg
     2641 gcggcgattg ggctgcggat aagattgtcg gtgcggcaga aacgttggcg cgcggcggtc
     2701 aggtgttttc aattccgttt ctgcaccaga cttcgacaac gaagactttg gcaaaaatcc
     2761 gtgcggcaga gggcggaaaa tgacggtttt gaagctttcg cactggcggg tgttggcgga
     2821 gcttgccgac ggtttgccgc aacacgtctc gcaactggcg cgtatggcgg atatgaagcc
     2881 gcagcagctc aacggttttt ggcagcagat gccggcgcac atacgcgggc tgttgcgcca
     2941 acacgacggc tattggcggc tggtgcgccc attggcggtt ttcgatgccg aaggtttgcg
     3001 cgagctgggg gaaaggtcgg gttttcagac ggcattgaag cacgagtgcg cgtccagcaa
     3061 cgacgagata ctggaattgg cgcggattgc gccggacaag gcgcacaaaa ccatatgtgt
     3121 gacccacctg caaagtaagg gcagggggcg gcaggggcgg aagtggtcgc accgtttggg
     3181 cgagtgtctg atgttcagtt ttggctgggt gtttgaccgg ccgcagtatg agttgggttc
     3241 gctgtcgcct gttgcggcag tggcgtgtcg gcgcgccttg tcgcgtttag gtttggatgt
     3301 gcagattaag tggcccaatg atttggttgt cggacgcgac aaattgggcg gcattctgat
     3361 tgaaacggtc aggacgggcg gcaaaacggt tgccgtggtc ggtatcggca tcaattttgt
     3421 cctgcccaag gaagtagaaa atgccgcttc cgtgcaatcg ctgtttcaga cggcatcgcg
     3481 gcggggcaat gccgatgccg ccgtgctgct ggaaacgctg ttggtggaac tggacgcggt
     3541 gttgttgcaa tatgcgcggg acggatttgc gccttttgtg gcggaatatc aggctgccaa
     3601 ccgcgaccac ggcaaggcgg tattgctgtt gcgcgacggc gaaaccgtgt tcgaaggcac
     3661 ggttaaaggc gtggacggac aaggcgttct gcacttggaa acggcagagg gcaaacagac
     3721 ggtcgtcagc ggcgaaatca gcctgcggcc cgacgacaga ccggtttccg tgccgaagcg
     3781 gcgggattcg gaacgttttc tgctgttgga cggcggcaac agccggctca agtgggcgtg
     3841 ggtggaaaac ggcacgttcg caaccgtcgg tagcgcgccg taccgcgatt tgtcgccttt
     3901 gggcgcggag tgggcggaaa aggcggatgg aaatgtccgc atcgtcggtt gcgctgtgtg
     3961 cggagaattc aaaaaggcac aagtgcagga acagctcgcc cgaaaaatcg agtggctgcc
     4021 gtcttcggca caggctttgg gcatacgcaa ccactaccgc caccccgaag aacacggttc
     4081 cgaccgctgg ttcaacgcct tgggcagccg ccgcttcagc cgcaacgcct gcgtcgtcgt
     4141 cagttgcggc acggcggtaa cggttgacgc gctcaccgat gacggacatt atctcggggg
     4201 aaccatcatg cctggtttcc acctgatgaa agaatcgctc gccgtccgaa ccgccaacct
     4261 caaccggcac gccggtaagc gttatccttt cccgaccaca acgggcaatg ccgtcgccag
     4321 cggcatgatg gatgcggttt gcggctcggt tatgatgatg cacgggcgtt tgaaagaaaa
     4381 aaccggggcg ggcaagcctg tcgatgtcat cattaccggc ggcggcgcgg caaaagttgc
     4441 cgaagccctg ccgcctgcat ttttggcgga aaataccgtg cgcgtggcgg acaacctcgt
     4501 cattcacggg ctgctgaacc tgattgccgc cgaaggcggg gaatcggaac atacttaaaa
     4561 aatacaccct ttttcaacac ggggcggcat ccgtccgaat ccgtccggat tctgccgccc
     4621 gacagactgc cgcccaggcg ggcggggttt caaacattga ggaaattcta atgaaatggc
     4681 tcttcaccct tttggcggta ctcaatattg ccgtattcgg cggcacggtg ggctataaat
     4741 tggccgttaa agcgacgggc ggcgtgccgg aaaaccgggc cgtcgaaaac acgccccccg
     4801 caacgcccgc cgccgacaat gcggcggcat ccgtggagga tacggcggca ctgctcaaac
     4861 cgggcgacat cctgaccgaa gagcaggccg agcagttgcg cttgaaaaag gaagcggaac
     4921 agaaaaaact gagggagaaa aaacagcgtg aagaaaaagc ccgccgcgaa aaactcgccg
     4981 ccgaaaaggc gcaggcggaa cgcgaaaacg gcgcggcgga tgccttatgc gccgcgcagg
     5041 caagcctcac gatggacgaa gacgactacc accgcatcaa agggcttttg ggcaaatggt
     5101 cgcacgttgc cagcaggagc gttgaaaaac gcaccgccaa agccaaacct gccgacaaaa
     5161 cctaccgcgt cgtcctgccc gtttccgccg atgccgaaaa tcaggcggcg gagctgtctg
     5221 ccaaaggttt caaccccata ccgtttgacg gcgcattgag tttgggtgtc ggcaacagtc
     5281 gggaaaacgc ccaagccctg caaaaccggc ttgccggcgc cggattcggc ggggcgcata
     5341 ttgtcgaaca ctttgccgaa gccgacaggc aggacgattc tttgagcgtg tcgcgtatga
     5401 cggttttgtt taccggcgtg aatgccgccg atgcggacga aatccgtaaa atcacgtccc
     5461 tatacggcaa actgaacctc aagtcttgca aataggcgcg aaagccggac gataaacgcc
     5521 aaacccaccg cgaaggtggg tttggcgttt cggttttgag tatctgatat ataaaaaggg
     5581 aatgtatgtc tcgtatatgc cgactccaag tgtgaaagtg atgatgggga aatatggtgt
     5641 ttaatggttc ggaacggacg gcttccgagt ggttttgatt atacgctaat aataataatt
     5701 atcaataaaa atttaaaaat aaatgcagat tttttgtaat acgatgaaaa ataaacaaat
     5761 taacctgtcc gccgatgttt gtgattcggg tcggcaaact ttcattgtgt cggacaaatc
     5821 ggggtcggca ggcatagctg agggcttcgt gcctgacgga aataagcctg ccgtgtttga
     5881 acatccgtcc gatttggcgc ggacagggtt gtcattcggg ggtgtttgag cgtggattcg
     5941 gggatgcaaa agcggtaaaa ccgttgcgga tgaaggctta cggacggctt taggtatttt
     6001 taagtgtatt caaacaacaa caaaacgctt cttctattgc cgtatttagg gcttgcgcgg
     6061 cagcctgcac accgtggagg ccgagtgttt cgttggttgc ggcatcggga cgggcggcaa
     6121 ggttttccgg ccggtttttc cattttttcc gacagagccg tgatgcttgg gaatggcggt
     6181 ttgcccgttt cagaccgccg ccgttttggg ctgcgatgcc gtctgaaccg ttcagacggc
     6241 atttttgccg cctttttcaa tattccgccg aatgccaaaa cggctgtccg actgtcggcg
     6301 tgctggcttg cgctttgtcg cgtgcttcga ccggtccggc ttcaaatgcc agccgtccgg
     6361 attcgacggc gagtgcgaag gcgcgtgcca tattgaccgg atcgccgctg cgggaaacgg
     6421 cagtattcaa aagcacgccg tcaaaacccc attccatcac ttgtgccgcc tgtgagggca
     6481 aacccaagcc cgcgtcgata atcagcggcg tgtcgggcag gcgttcgcgc aggacgttca
     6541 acgcgtaggc gtgaaccgcg cccaaacccg tgccgatcgg agccgcccac ggcattaacg
     6601 cctgacagcc cacatcgagc aggcggcggc aggcaatcag gtcttcggtg caataaggca
     6661 gcactttgaa gccgtcttta atcaggattt ccgccgcttc gacaagttgg aacacatccg
     6721 gctgcaaggt gtcgtcgtcg ccgatgagtt cgagtttaat ccaatcggtt tcaaacactt
     6781 cgcgcgccat ttgcgccgtc gttaccgctt cctgcacgct ttggcagcct gccgtgttcg
     6841 gcaggacggg aacgccggtt tcttgaagca gcgaccaaaa cccctgaccg tgcgcctcgc
     6901 cgccgcttcc cgtgcggcgc agcgagacgg taatcatcgc gggttgggcg atacggacgg
     6961 attgtttgag gatttcaggg gtcgggtagg cagccgtgcc gagcagcagc cgtgaaggga
     7021 aagtttctcc gtatagggtg agcataatgg gttc
//
LOCUS       DAWFBO010000104         6770 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWFBO010000104 DAWFBO010000000
VERSION     DAWFBO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.104"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(130..489)
                     /gene="rplT"
                     /locus_tag="WGO87_001492"
     CDS             complement(130..489)
                     /gene="rplT"
                     /locus_tag="WGO87_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0392839.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(502..699)
                     /gene="rpmI"
                     /locus_tag="WGO87_001493"
     CDS             complement(502..699)
                     /gene="rpmI"
                     /locus_tag="WGO87_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0392840.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(846..1367)
                     /gene="infC"
                     /locus_tag="WGO87_001494"
     CDS             complement(846..1367)
                     /gene="infC"
                     /locus_tag="WGO87_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0392841.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1385..3298)
                     /gene="thrS"
                     /locus_tag="WGO87_001495"
     CDS             complement(1385..3298)
                     /gene="thrS"
                     /locus_tag="WGO87_001495"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0392842.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPITEDSSVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWDKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3351..3427)
                     /locus_tag="WGO87_001496"
     tRNA            complement(3351..3427)
                     /locus_tag="WGO87_001496"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3391..3393),aa:Val,seq:gac)
     gene            3606..4721
                     /gene="tgt"
                     /locus_tag="WGO87_001497"
     CDS             3606..4721
                     /gene="tgt"
                     /locus_tag="WGO87_001497"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0392843.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQAQFHENRARGTD"
     gene            4731..5813
                     /locus_tag="WGO87_001498"
     CDS             4731..5813
                     /locus_tag="WGO87_001498"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0392844.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            6005..6406
                     /locus_tag="WGO87_001499"
     CDS             6005..6406
                     /locus_tag="WGO87_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0392845.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(6469..6759)
                     /locus_tag="WGO87_001500"
     CDS             complement(6469..6759)
                     /locus_tag="WGO87_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392846.1"
                     /translation="MAKYFTETGIGVREHFDFFGEFVVSPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVPR"
BASE COUNT         1718 a         1822 c         1582 g         1648 t
ORIGIN      
        1 aatttaatcc actatatttt aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa
       61 aatcaatcac atagaatttc tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa
      121 ttttttggat taagcagcca aagcagcttt ggctttttca accaattgtg caaaagcggc
      181 tttatcgaac acggccaaat cagccaatac tttgcggtcg atttcgatag aggcgcgttt
      241 cagaccattc ataaatttgc tgtaagacaa cccgttttca cgcgcacctg cattgatacg
      301 gacaatccac aattgacgga attggcgttt gcgttggcgg cggtcgcggt atgcgtattg
      361 gccggctttc attaccgcct gcttggcaac gcggtaaacg tttttacgac ggccgcggta
      421 gcctttggct aacgcgaaga ttttttggtg acgggcacgg gcggtaacac cgcgttttac
      481 gcgtggcata ttctaaactc cttaagcgta gggtaacatt ttagcaacag aagccaaatc
      541 gcgatcgttt accatagagg taccgcgcag ttggcgtttg tttttggtgg tctttttagt
      601 caagatgtgg cgtttgaacg catgagcgcg tttcacaccg ccgttaccca gtactttaaa
      661 gcgttttttc gcgctagact tggttttcat tttaggcatg ggaaaactcc attcgttatc
      721 ggataaggca ttagggggtt ttaaaacatc agtttcaaac acttgaaccc acaataccac
      781 ggttttttgc cgcaattgaa aacttactcc gaagcggcaa tcggagtaag cggagaatta
      841 tagctttatt ttttcttcgg cgcaatcatc atcaccattt ggcgaccttc cattttggga
      901 aaggactcga tttgcgccac ttcagccaaa tcttctttta cacgttccaa aagttgcgcg
      961 ccgagttgct ggtgagccat ttcacggccg cggaaacgca atgtcacttt gactttatcg
     1021 ccgtcggcaa ggaagcggtt aatgttgcgc atcttgattt gataatcgcc ctcatccgta
     1081 cccggacgga atttaatttc cttgatttgt acctgctttt gatttttctt ggcttcgtcg
     1141 cgcttcttgg cttgttggta tttgtattta ccgtaatcca tcagtttgca tacaggcggt
     1201 ttagcagttg gggaaatctc taccaaatcg acatcctgcc cttcggccat agccaaagct
     1261 tcacgaactg aaacgacacc aagctgttcg cctgactcac tgattaaacg cacttctttg
     1321 gcggtaattt cgccgttgat tcgtgcttcg cgttcttgag cgatgatgaa tactcctata
     1381 aaaattaatg attgacgagg gcatcagtga tttcttgctg caattgcgca atgaaatcat
     1441 ccaaatccaa agaacccaaa tcttctgctt tgcggcgtac cgccactttg ttttcctgct
     1501 tttctttatc gccgacaacg atttgataag gaaaacggta ttggctgttg tcgcggattt
     1561 tgtaaccgat tttttcgtta cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt
     1621 tggcagccac ttcacggcag taatccgctt gattttcggt gatattcata atcaccattt
     1681 gaaccggcgc cagccataac gggaaagagc ctgcatggtt ctcaatcaga atgccgataa
     1741 accgctccaa agaacccaaa atggcgcggt gcaacattac agggcgagcg cggtcattgt
     1801 tttcggttac atattctgca tccaagcgtt ccggtaagac gaaatctagt tgcaatgtac
     1861 cgcattgcca agaacgaccc aaggcatctt tgacgtgata ttcgatttta ggcccgtaaa
     1921 acgcaccttc gccaggcaac tcttcccact ctacgccaca agcggtcaat gcatcacgca
     1981 agccttgttc ggccttatcc caaacttcgt cagaacctgc acgttgttca gggcggaggg
     2041 acaacttcac agcaacatca tggaaaccga actgtttgta gatgcgaacc aacaattcat
     2101 tgaatgcgcg cgcttcgctg acgatttggt cttcggtaca gaaaatatgc gcatcatctt
     2161 gcacaaaacc gcgaacacgc atcagaccat gcaatgcacc gcttggttca ttgcggtggc
     2221 aagaaccgaa ctcggccaaa cgcatcggca aatcgcgata tgagcgcaaa ccgttattaa
     2281 aaatttgcac atgacccgga cagttcatcg gtttcaccgc gtattcgcgt ttttctgaac
     2341 tggtcacgaa catattgtct ttgtagttgt cccaatggcc ggatttttcc caaaaggttt
     2401 tatccataat ttgaggcgtt ttgacctctt tataaccggc ggcgttcagc tctttacgca
     2461 tatgctgttc aatcacttgc cacaaagccc agcctttagg atgccaaaac accatgcccg
     2521 gcgcttcgtc ttgcagatgg aacagatcca attgcttgcc aagtttgcgg tggtcgcgct
     2581 tttcggcttc ttcgatacgt tgaatatagg ctttcaactc gtcttttgtc gcccaagccg
     2641 taccgtatat gcgttgcagc atttcattgt tgctgtcgcc gcgccagtat gcgcccgcca
     2701 acttggtcag tttgaagttt ttcaggaaac gggtattcgg aacgtgcggt ccgcggcaca
     2761 tatcgacata ttcctgatga tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa
     2821 tcaggcgcag tttgtattct tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg
     2881 gagtcatgat tttgaccaca tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa
     2941 tggcggcaac atcttccggt gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct
     3001 cttcaatgac ggggccgata accatttttg cattaggata gagttgcttg acggcatgcc
     3061 cgacaagatg cgcgcaggaa tggcggatga tttcgatgcc ttcctgatct ttcggagtaa
     3121 tgatttggac gcttgaatct tcggtaatcg gatcgcacgc atcgaccaat ttgccgttta
     3181 ccttgcctgc cactgccgcc ttcgccaaac cggcaccgat agacgcagca atttgagcca
     3241 cggtaacggg ggattcgtat tggcggactg agccgtccgg caaggtaata ttcaacatca
     3301 aacgctccga aagattaaaa aataacggca taaatgccgt tatggggatt tggtaggcac
     3361 gattggattc gaaccaacga cccccaccat gtcaaggtgg tgctctaacc aactgagcta
     3421 cgtgccttca aagaattttt gcattttatc gggtcgtttt aatttttgca agaggtatgt
     3481 gcatttcttt atgcaacatt gtggcaagat ttggggatgt cgtctgaaaa accgtgggaa
     3541 ttcatttata atgccgtctt ttaaaacata tttcgccccc tgtcgcccaa ctgacattca
     3601 atcttatgct taaatttact ttacacaaaa aagacggata cgcccgacgc ggcacgctgg
     3661 agttgaacca cggcaaaatt gaaacgccgg tatttatgcc tgtcggtact tacggctcgg
     3721 ttaaggcgat gaacccgcaa aacctgcacg acatcaaggc gcaaattatt ttgggcaaca
     3781 cttatcattt gtggctgcgt ccgggtttgg aagttatcgg gcaattcggc ggcctgcacg
     3841 gttttatcgg ctgggacaaa ccgattttga ccgactcggg cggttttcag gttttttctt
     3901 tgtcggatat gcgcaagctg acggaagaag gctgtacgtt caaaagcccg attaacggcg
     3961 acaagctgtt tttatcgcct gaaatttcta tgaaaatcca aacggttttg aactcggaca
     4021 tcgcgatgca gttggacgaa tgcacgccgg gcgaggcgac gcgcgaacag gcgcgaaaat
     4081 cgctgcaaat gagcctgcgc tgggcggaac ggagcaaaaa agcctttgaa gatttgaaaa
     4141 acccgaacgc actgttcggc atcgtgcaag gcgcgatgta tgaggatttg cgcgaagaat
     4201 cgctgcgcgg tttggaacag tttgattttc ccggccttgc cgtcggcggt ttgtccgtcg
     4261 gcgaacccaa gcccgaaatg taccgtatgc tgcgcgccgt cggtccgatt ctgccggaac
     4321 acaagccgca ttacctgatg ggcgtaggca cgccggaaga cctcgtgtac ggcgtggcac
     4381 acggcatcga tatgttcgac tgcgtgatgc ccacccgcaa tgcacgcaac ggctggctgt
     4441 tcacccgttt cggcgatttg aaaatcaaaa acgccaaaca caagctcgac aagcgtccga
     4501 tagacgaaag ctgcacctgc tacgcctgcc aaaacttcag ccgcgcctac ctgcaccatc
     4561 tgcaccgcgc cggcgaaatc ttgggcgcac agctcaacac catccacaac ctgcatttct
     4621 accaagtcat tatggcggaa atgcgcgatg ccatagaaca aggcaaattt gccgactggc
     4681 aggcacaatt ccacgaaaac cgcgcacgcg gcacagactg attcccggca atgccgtctg
     4741 aagccgcttc agacggcatt ttgccgcttt accctacccg cactattttt cagaaaccga
     4801 tcatgcttcc attccttcca gaaccctccc tttcctatac gcaacaaaac cgtaccgccg
     4861 ttttgctgtt gaacctcggc acgcccgacg cgccgaccgc gcaggcggta cgaccttatc
     4921 tgaagtcctt tctgaccgac cggcgcgtcg tcgaactgcc caaatggctg tggtatccca
     4981 ttctgcatgg tctggtactg acgttgcgcc ccaaaaaaag cgcgcacgcc tatgaaaaaa
     5041 tctggtttaa ggaaggttcg cccctagaag tttacacagc acgccaagct gccgcgcttg
     5101 ccgaacgtat gcccgacctc atcgtccgcc acgccatgac ctacggcaat ccttcggtgg
     5161 cagacgtatt gtcggaactg aaggcgcaag gcgcgggcag actgctggtc atcccgatgt
     5221 acccgcaata tgccgcttcc agcagcggcg cggcagtaga taaggtgtgc gaacaattac
     5281 tgctgcaacg gaaccaaatg agcgtccgca ccgtttcgcg cttttatgac gataccggct
     5341 acattgatgc gatgaaaaac cacatcctcc gatattgggc ggaacacgga cgtgggaaaa
     5401 aactgatgtt gagttttcac ggcgtaccgc agaagcacta cgacctcggc gacccctatc
     5461 ccgacgagtg ccgccacacc gccaaactgc ttgccgaagc actggaactg accgaagacc
     5521 aatatgtcgt atcgttccaa agccaattcg gcagggcaaa atgggttaca ccgagcacgc
     5581 aggatttgtt cggcaaactg cccaaacagg gggtaaccga gctggacgta ttctgcccgg
     5641 gctttttggc agactgtttg gaaaccatgg aagaaatcgc cctgatgggg cgggaacagt
     5701 tttacgaagc aggcggcaaa agctaccgct acatcccctg cctcaacgac aaccccgact
     5761 ggatagacgc actcgtcgca cttgccgaag aaaaccttgg cggctggcgt tgaaactgtt
     5821 tgacccatgt ctaaaatgcc gtctgaacgc cgtgttcaga cggcattttc ccgctacttt
     5881 agcgtatgat ggcatctatg ccggacaaca acatcaagtg caaccttact accgattcgg
     5941 caaaaaaatc tttcacccgc tacgcaaaat caacaacaag gtttcaaaca ttaaggaaac
     6001 accaatgaac acaacccgac tgccgaccgc cctcgtcttg ggctgcctct gcgccgccgc
     6061 ttctgccgcc gacaacagca tcatgacaaa agggcaaaaa gtgtacgaat ccaactgcgt
     6121 cgcctgccac ggcaaaaagg gcgaaggccg cggaaccatg tttccgccgc tctaccgctc
     6181 cgacttcatc atgaaaaaac cgcaggtgct gctgcacagc atggtcaaag gcatcaacgg
     6241 tacaatcaaa gtcaacggca aaacctacaa cggattcatg cccgcaaccg ccatcagcga
     6301 tgcggacatt gccgccgtcg ccacttatat catgaacgcc tttgacaacg gcggcggaag
     6361 cgttaccgaa aaagacgtaa aacaggcaaa aggcaaaaaa aactaaacag acaaaatgcc
     6421 gtctgaaacc ggcaatccgg cttcagacgg cattcaaatc aaacctttct aacgcggtac
     6481 tttcagcccc atagacccga tattgccgcc accgtcaacc tccttcgcgg tcagcataaa
     6541 gccgccgatg tcaatacggc cgcactcgac cggctcctga ctgtcggaac gcttatcgaa
     6601 aagctcggca aggctcaaac cctcttcgcc cgcttccagc ctcaaaccgt aagcaagtgc
     6661 caaatcaccc gaacgtgctg ccggcgaaac gacaaactcg ccgaaaaaat caaaatgctc
     6721 acggacaccg atacccgtct cggtaaagta tttcgccatt ttatagtgaa
//
LOCUS       DAWFBO010000105         6712 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFBO010000105 DAWFBO010000000
VERSION     DAWFBO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.105"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(42..809)
                     /locus_tag="WGO87_001501"
     CDS             complement(42..809)
                     /locus_tag="WGO87_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0392847.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WGO87_001502"
     CDS             complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WGO87_001502"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0392848.1"
                     /translation="MPELPEVETTLRGIAPRIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1776..2441)
                     /locus_tag="WGO87_001503"
     CDS             complement(1776..2441)
                     /locus_tag="WGO87_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEZ0392849.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSVDMLLFPHTLEGGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPEWDGYLGGKALAAGAAR
                     VAD"
     gene            2620..4596
                     /locus_tag="WGO87_001504"
     CDS             2620..4596
                     /locus_tag="WGO87_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0392850.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDIYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIVRLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSSQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4664..5356
                     /locus_tag="WGO87_001505"
     CDS             4664..5356
                     /locus_tag="WGO87_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEZ0392851.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            5853..6455
                     /locus_tag="WGO87_001506"
     CDS             5853..6455
                     /locus_tag="WGO87_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0392852.1"
                     /translation="MPTLSQGLPNNYIYRENGVEFGLVQIRYPEHIELLHRWMNMPHV
                     IPQWQLNKSLSELSVYFEKMLADDHQRLYLVSINGEWVGYAEVYEGARDRLSGYYTAD
                     KNDLGWHLLLAEAQAVGKGYLRAIMRLLSFFIFEHSPAKKIVGEPDASVKPYEVVAQE
                     LNYMPQYQIVMPEKTAMLYFCDKEGFYCKFAHYLNASIKE"
     gene            6460..>6712
                     /locus_tag="WGO87_001507"
     CDS             6460..>6712
                     /locus_tag="WGO87_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392853.1"
                     /translation="MPIFLNAIQNPNSSLPVFLLDFSGEISQDAFDDWLIAMSDLLAS
                     EQSFALIYHSRTGLTLPNNYRQIEAVWYKTHKESFFKYCK"
BASE COUNT         1829 a         1974 c         1428 g         1481 t
ORIGIN      
        1 ccacaaaagc gaatcatact gccacattcc gacaaccggt ttcagacggc aatatccgca
       61 tcgtcggcga caacggcacg gatgctttct tcgattttat cttttaaagc ataacggtct
      121 tcgctttccg ccgcgtccgc cacgcaaacg aaatcgactt ttatcgttaa ttttttcata
      181 gacacaatgc gccacagaca ggtcggcaaa ccgacatcgg catatgaggg acgagccgtc
      241 ctttttcccg tttcgtcata ataacgcagc gcgaccgcca aaacctttgc ccccgcatca
      301 atggcggatt ggaacagcgc ggctttgaac ggcaaaagcc ccaatccgga ggaagtccgc
      361 gcttcgggga aaaaactgac gttttgaccg cgttgcaagg tttcgcagac ggcgcggtta
      421 atcggttcga tgtcgcgccg cgaattgcgg ttgatgaaca ccgttcccgc gttctgcccc
      481 atcttgccca ataccggcca gcttttgatt tcctgcttgg cgataaagct gctcggataa
      541 accgcgctca tcgcgaaaat atccagccag gacacgtggt tggcggcaac caagacaccg
      601 ttcggatgtt cgggtgcggg tctgcccacc tccaatccga tatccaaagc cgccaaaacc
      661 cccctgccca actcgattac cgcccgattg cgcgactcgg ggcaaccgcc gtcaataccg
      721 cgcaggtttt tcccggtttt gaacagccag accgccaaac ggcacaagcg gcgcagacgt
      781 gtaaaaaatg aagctttatt tgaagacatg agcaattctt tcccgataat cggtgtttgt
      841 tgttgaaacg ttcggacggc ataccgataa aatgccgtct gaaaccgttt tcagtcctat
      901 ttctgacagt tcgggcaata aaacgtgccg cgctgcccca aagtttcttt cacaaccaaa
      961 ccgccgcacc gggggcacgg ctgattgtgc cgcccgtaca ctgtatattc ctgttggaag
     1021 tagccgcttt tgccgtcgct gtccacaaaa tccctcaagg tactgccgcc cgtttcaatg
     1081 gcgcgctgca acaccgcttt gacggtttca accaaaagcg cgcactcttt ctttttcagg
     1141 cggttggcag gacggtgggg cgaaatgccc gctctgaaca ggctctcgtt ggcataaatg
     1201 ttgcccacac cgaccacgac cgcattgtcc atcagggcaa gtttgaccgc gcgcttctgc
     1261 gccttcagcc ttgcatacag ataatccgca caaaatgcct ccgacaaagg ctccggcccc
     1321 agtttttcca acagcggatg atgttcttcg attccctcat accaaagtat cgcgccgaac
     1381 tttctcggat cgcggtaacg catgaccgtg ccgtctgaaa acacaatatc gacgtgatcg
     1441 tgtctgtccg gcctgccgat acgtccgtcc gacggcgtaa aaatccgcaa gctgcccgac
     1501 atccccaagt gaatcagcaa aatccccgtt tggaaacgga caatcagata cttcgcccgc
     1561 ctgccgcagg acaacacctg ccggccggac aaaatctccc ccaaatcggg attaatctgc
     1621 cagcgcagct tcaattggcg caataccacg gcttccaccg ttttcccttc aatacgcggc
     1681 gcgatgccgc gcaacgtcgt ttccacttcc ggcaattcag gcataacccc tcccgacatt
     1741 tcttctgaca gatgccgtct gaaagacggc tgtccctaat ccgcaaccct tgccgcaccc
     1801 gccgcaaggg ctttgccgcc caaatacccg tcccactcgg gcaggggcgt tacgcccgcc
     1861 gcccttttga ccaaaaccaa accgtacact gcggcacact gcggccacca acggtcgccc
     1921 gccttttcca taaaccgcca aaagcgtatt tgcccgagcg acgaaacggg cggcagatac
     1981 accataaatt tcccaaattc aatatcgaaa ccggcatcgg caagccgcct tttcaactcg
     2041 ggcaacggca gacaaaaccg tttttccggc aggcgttcgc cgtcaaacca acggctgaat
     2101 ccccagagcg aatacggatt gaaacccgtc agcatcaaac gtccggacgg tttcaatatc
     2161 cggtgcgcct cggacaggat ttgcgaagga acaccgcctt ccaaagtatg cgggaaaagc
     2221 agcatatcca cagaaacatc cgccaaagcc atattttccg ccgacatcga catatctcgc
     2281 ggcacacaga caacatcttc agacaggctc agccacctgc cgcccacctg aaccgcacac
     2341 attcccgaaa aacggtatga atccagatac cgcccgaaga aatcctgttc caattttgca
     2401 acataccgcc ccatcgccgt atcttcaaac catgcatcca tattgcgtcc gtttcaaaca
     2461 gattgcctgc cgatattcta ttccaaacag gatttctgtc aaaaaacaca tcggccgccc
     2521 atttccgaat ccgcataaag ttcctgtaaa acttgacgct ttttcagtca aacagtacca
     2581 tcggacgata aaatatgttt attccgccga atataaatca tgtccaaact caaaaccatc
     2641 gctctgaccg catcaggtct gtccgtttgt ccgggtttcc tatacgccca aaacacctca
     2701 tcacaccaaa tcggtttggc gattatgcgc ttaaactctt caatactcga cctgcccccg
     2761 acaaaacaat atttccaatc cggcagcctg tggagcgagc tgcgccaagg cttccggatg
     2821 ggcgaagtca atcccgaact ggtacgccgc cacgaaagca aattcatcgc aagccacagc
     2881 tatttcaaca gggtcatcaa ccggagtaga ccctatatgt accatatcgc caacgaagtc
     2941 aaaaaacgca atatgcccgc cgaagccgcc ctgcttccct tcatcgaaag cgcgttcgtc
     3001 accaaagcca aatcacacgt cggcgcatca ggattatggc agtttatgcc cgctaccggc
     3061 aggcattacg gcctggaaaa aacaccggtt tacgacggca ggcacgacat ttacgccgcc
     3121 accgatgccg cactcaacta tctgcaatac ctctatggac tgttcggcga ctggccgctc
     3181 gcctttgccg cctacaactg gggtgaaggc aacgtcggac gcgccatcaa ccgcgcccgc
     3241 gcccaagggc tcgaaccgac ctacgaaaac ctgcgtatgc ccaacgaaac gcgcaactat
     3301 gtccccaagc tgctcgccgt gcgcaacatt attgccactc cccaatcttt cggcatgaat
     3361 atcagcgaca tagacaacaa accctatttt caggcagtcg aaccggatcg tccgctcgac
     3421 aacgaagcca tcgtccggct tgccggcatc acgcaaagcg agctgctcgc cctaaacccc
     3481 gcattcaacg tccccgcgtt tatccccaaa agcaaacgca aactgctgct tcctgtcgcg
     3541 tccgtacaaa ccttccaaag caactacctc aacgccgcac ccgacagcct gttttcatgg
     3601 gaagtctata cgcctgccgc caaaaccagc ctgtccgaca tctcgacggc aaccggcatg
     3661 agcattgccg acatcaaacg cctcaacaac ctgaacggca accttgtcaa cgcaggacgc
     3721 agcatccttg tcgccaagaa cggcaaaacc cttcagacgg catcggaatc cgtcgtttcc
     3781 atcgacatcg acaatacgcc cgacacctac cgttccaata tgccggcagg cacggtgaac
     3841 gtcggcattg cccgaatccg acccgccgcc gcacagacag cggacattac cgtcgcacct
     3901 ttgccgcaga aaaccgtccg tacggaaccc gatccccttg tccgcattgc cgaacctgcc
     3961 cttgcgacag ccgcagcgca acctcaaacc gaaaaacaga ccgccatgcc gtctgaaacc
     4021 caaaccgcaa cactcgcgca gatcatcccc caaaacgaca tgcaggcggc agacgaactc
     4081 atgcagcttg ttgcccgaaa caacctgcgc cgacaggctg aagaaaccat ctccgccgtc
     4141 atcggcacgc ctgacacagt tgccgaacac aacatttcct catcttcgca acataccgtt
     4201 gccgccgacg gcaaacgccg ggcacgtttg gaaacgcgcg tagccaaagc tgccgacggc
     4261 gaagccgaaa tctccccgct ccatgccagc atccaccgcg ttgtagaagg cgacaccctg
     4321 ttcaacattg ccaaacgcta caacgtcagt gtagccgacc tgattgtcgc caacaacatc
     4381 aaaggcaaca ccatccaaaa aggacaggta ctccgcctgg cgcaggcagc ccctgcccaa
     4441 acccgtattg aaaaagtatc ctacaccgcg cgcaaaggcg acaccttcaa aagtatcgcc
     4501 gcgcgcttca atatccatat cgacgacatc cgccggctca atcccaacct gaacaccatc
     4561 aatccaggac agagggttaa actgattgga agctgattcg gatacggcac atgacaggac
     4621 tttctcagtc ctgtcttttt catcccacat ttcccatacc atcatgaaac tcatcaaata
     4681 cctgcaatat caaggcatag gaagccgaaa gcagtgccaa tggctgatta ggggcggcta
     4741 cgtctccatc aacggaacct gtatggacga caccgatgca gacatcgatt cctcatccgt
     4801 cgaaacgttg gatattgacg gggaagcagt aaccgtcgtt cccgaaccct atttctacat
     4861 catgctcaac aagcctgaag attacgaaac ttcgcacaaa cccaagcact accgcagcgt
     4921 attcagcctg ttccccgaca atatgcggaa catcgatatg caggcggtcg gcaggctgga
     4981 tgcggatacg accggcgtat tgctgattac caacgacggc aaactgaacc acaacctgac
     5041 ttcgccgagc agaaaaattc ccaagctata cgaagtaacg ctcaaacacc ccacaggaga
     5101 aacgctctgc gaaaccttga aaaacggcgt gctgctccac gacgaaaacg aaaccgtttg
     5161 cgccgctgat gccgttttgg aaaacccgac caccctgctg ctgaccatta ccgaagggaa
     5221 ataccaccaa gtcaaacgca tgatcgccgc cgccggcaac cgcgtgcaac accttcatcg
     5281 ccggcgattc gcacatctgg aaacagaaaa cctcaaaccc ggggaatgga aatttatcga
     5341 atgtccaaaa ttctgaaata acatccaaga attccattta tattcatcca catactcatt
     5401 aaatatatgg tttaacccaa tttaacgcaa aaaatcaatt aatgagttat caaagattat
     5461 tgatgaattt caccatcaac atccagagct aaatgagagg attgctttat ttgatttaaa
     5521 aagaccaaaa tttgaaaagg tgtgcctaaa tcgtgttcgc ttttttacaa gaggttatca
     5581 agataacgcc aaccgtccag aacccgtggt ttgcgaacca atttgcaatc caatatcacc
     5641 acagtttcta aggtgtgttg aacattgata acattatttg taaaaatgat gaaaatttta
     5701 acgcttttat tagtttttgc atgaaaaacg tgcgattttt ctcatttttc attgcaaata
     5761 ccaaatattc aacacaccct aatgagagaa tagaaatgct agaaatctat accgattctt
     5821 ttaatcaaga tcaatgcagt aaaatcaatc gattgccaac tttatctcag gggttgccaa
     5881 acaactatat ttacagagaa aatggggttg aatttgggtt ggtgcaaata cgctaccctg
     5941 aacatattga attgttgcat cgttggatga atatgcccca tgttatccct caatggcaac
     6001 tcaataaatc attgtctgag ctgtctgttt attttgaaaa aatgttggct gatgatcacc
     6061 agcgtttgta tttggttagt attaatggcg aatgggttgg ttacgccgaa gtttatgaag
     6121 gggcaaggga tagactatca gggtattata cagctgacaa aaatgatttg ggttggcatt
     6181 tgttactagc agaggcacag gctgttggca aagggtactt gcgtgccatc atgcgtctac
     6241 taagcttttt tatttttgaa cattcacctg ctaaaaaaat tgtgggtgaa cctgatgcaa
     6301 gtgtaaaacc ctatgaggta gtcgctcaag aattaaacta tatgccccaa tatcaaattg
     6361 taatgcctga aaaaactgca atgttatatt tttgtgacaa agaagggttt tattgtaagt
     6421 ttgcccatta tttaaatgcc agcattaaag agtgatgtta tgcctatttt tctaaatgct
     6481 attcagaacc caaatagctc cttacctgta tttttactcg atttttcagg cgagattagt
     6541 caagatgcat ttgatgattg gttgattgct atgagtgatt tgttagccag tgagcagagt
     6601 tttgccttga tttaccatag tcggacaggg ctgacactcc ctaataacta ccgccagatt
     6661 gaggcggttt ggtacaagac gcacaaagaa tcgttcttta aatattgcaa gg
//
LOCUS       DAWFBO010000106         6677 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFBO010000106 DAWFBO010000000
VERSION     DAWFBO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.106"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(97..1230)
                     /gene="nrdB"
                     /locus_tag="WGO87_001508"
     CDS             complement(97..1230)
                     /gene="nrdB"
                     /locus_tag="WGO87_001508"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0392854.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1291..2478)
                     /locus_tag="WGO87_001509"
     CDS             complement(1291..2478)
                     /locus_tag="WGO87_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ0392855.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(2471..3484)
                     /gene="dcm"
                     /locus_tag="WGO87_001510"
     CDS             complement(2471..3484)
                     /gene="dcm"
                     /locus_tag="WGO87_001510"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0392856.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(3542..5821)
                     /gene="nrdA"
                     /locus_tag="WGO87_001511"
     CDS             complement(3542..5821)
                     /gene="nrdA"
                     /locus_tag="WGO87_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0392857.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTSQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPCVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            6350..6661
                     /locus_tag="WGO87_001512"
     CDS             6350..6661
                     /locus_tag="WGO87_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ0392858.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         1782 a         1493 c         1418 g         1984 t
ORIGIN      
        1 aacaataacc gatcctaccc gctgtttttc ccatcataca acatacaaat gccgtctgaa
       61 acatccggct tcagacggca ttttttcaaa aaacgtttac aactcaaaat cgcccaaatc
      121 atccgtattc acttcagaat cgatctgacc gatcaaataa gaggatattt ccacttcctg
      181 cggcgcgacc tgtacgttat cggacgacag ccacgcatta atccacggaa tcgggttttg
      241 atttgcgcct tcaaatccgg ccggcaaccc caccgcctgc atacgcagat tggtaatata
      301 ttcgacgtat tgggataaga tttctttgtt caaaccgatc atcgaaccgt ctttaaacaa
      361 atatgccgcc cattcttttt cctgttccgc cgcttttttg aagagttgga aacattcgtc
      421 ctgcaactca gcggcaattt ctgccatttc agaatcatcg acacccgaac gcatcagatt
      481 aagcatatgc tgcgtgccgg tcaggtgcag ggcttcgtcg cgggcaatca gtttgatgat
      541 tttggcgttg ccttccatca actcgcgctc ggcaaaagca aacgagcagg cgaatgaaac
      601 gtagaaacgg atggcttcca acacgttgac gcacatcagg cagagataga gttttttctt
      661 caacccgcgc aaagacacag taatgggttt tccgccgaca ttgtgcaccc cttcgcccaa
      721 cagattgtaa tactgggtgt attcgattaa gtcatcgtaa tagcaggcaa tgtcttcggc
      781 gcgggcggta atgtattcgt tttcgacaat atcatcaaac acgaccgacg gatcattcac
      841 aatattgcgg atgatgtggg tatagctgcg cgagtggatg gtttcgctga agctccacgt
      901 ttcaatccac gtttccaact cgggaatcga aaccaaaggc agcaaagcaa cattcggact
      961 gcgcccttgg atggaatcga gcagtgtttg gtatttcaga ttgctgatga aaatatgttt
     1021 ttcgtgttca ggcaggttgg cgtagtcgat acggtcgcgc gacacgtcga tttcttccgg
     1081 ccgccaaaaa aacgacaatt gtttttcaat cagtttttca aatacctcgt atttctgctg
     1141 gtcataacgg gcgacattca caggctgacc aaaaaacatc ggctcattca gcgcgtcgtt
     1201 tttggttttg ggaaaggtgc tgtatgacat gactaggtgt tcgcaggaca taatctattc
     1261 tctatattaa ttaaaaatga tctgtaagtt ttaactaacc tgctgagact ttatatattt
     1321 aataaaattt tctaaagtta aaacatgggt tttcccaaaa gttccaaatg tttttacgga
     1381 ttctttaggg acaactaagg ttaaattctc atcactcatt tcctgaattt gagtatcaga
     1441 tataccttct tgtagagtaa ataaatgctt atgaggaatt cgatctgctt catttaaaac
     1501 ttggcgccag cgatccttac aagtagtctt tgaacctagc attgtcagct tttcagtttg
     1561 aaatatgaaa ttccctgatt catccatcgc atggtactct tcacttccag gaaataaaaa
     1621 atctggtttc tttttccctt ctgtcttcgc ttgcgtctca aattttaagg aaaattcgtc
     1681 aaatattctt gataaatgta actctaaaga ttttcctgct cttgcttttc tgcgatttgt
     1741 aaaagaatgt gcaaaattaa tgaagctatc caaatcagta atttttgatt taataatttc
     1801 aaattcacgt ttttcaaaaa tggaaaataa ttcatattcc tttgaaaccc attgttttaa
     1861 cacattacta acgttagttt tatttaatgc aatattttcc cttgccaaaa ctgccatttc
     1921 ttcagttttg gggaagttag gaaatagtga aaataatttt gttaggttat cctctacttc
     1981 ttgtttttta ggtaaatata aacttcctgg taaaatgtta gtttctgcga taaatatttc
     2041 aatatcttca tctgaagata aaacaaaagc atggaataat aaatctttat aacaagctct
     2101 gcacaatacc aataaggaac cactatattt atcacttaaa aattcaaaat tcttgccaaa
     2161 acccgtaatt ctcgcctcat ttctcgtgcc ttggccgtaa tatttaaaat tgctattagt
     2221 gacgcttcca tcttgccaat taattttgat tgaaatagtt ttatttgttc ccttttggca
     2281 aactacatca aagaggtcgt ctgaaaaatg tttttgaata taaaatcctg attggtgact
     2341 accagtagta ccaacatcat tgggacgaat ataacgacag tatactgcaa ttgatttatt
     2401 tgctgtctgt attgctgaag ctactaagtc atcggattta tttactactt gtattgtctg
     2461 agctgccaag tcattcattt tttaactcat ttataatttg tttagcaata gcttgaacca
     2521 acggtacagc aattgaattg ccaaactgct tgtatgcagc tgtcttggat actgcatcaa
     2581 taacaaaatc tttaggaaat cccattaaac gcgagcactc cctaggtgtc agcttcctag
     2641 gattttttcc tttctgaggg atgagtattt cggaaccatc tttgtaatat cgtgcagata
     2701 gagttcgtga tattccatct aaatcaacta atccaaaacc aaatccatta ccctttgcct
     2761 tatgtttttt agcgtaattt tgaaggtaaa gccataagtt atcagaaaga gtaaaagaat
     2821 tatctacatc atcttccaaa atttgcttta attttggttg tgattctggt ggggaaggaa
     2881 aattgaaatt tatttcctta ttaaaatatt gtctatcaaa acctacaata aaaatacgct
     2941 ccctattttg aggaacataa tattttgcat tcataacttg ataaaatatc tgatagtcaa
     3001 gctcttctaa agtcccttta attactttaa atgtatttcc tttgtcatgc gaaacaaggt
     3061 ttttcacatt ctctaaaaga aaaattttag gtcgatgttt tccaataatt tcagcaacat
     3121 caaaaaatag agttccctgc gccttatcta agaagcctgt ttctcgtcct aggctttttt
     3181 tctttgaaac accagctata gagaatggct gacacgggaa tcctgctgtt aatacatcaa
     3241 acttacttgg aatagctgct ttggtttcct ttaatgtaat atctccataa ggaatatcat
     3301 taaaatttac ttggtaggtt tgacgggctt tatcatccca ttcactagaa aatacacatc
     3361 gcccaccaac attctccatt gcaatgcgaa aaccacctat tcccgcaaat aaatcaataa
     3421 aagtaaattt ctcattattt aaggtaacta tattatctaa ttgattttgt attttatttt
     3481 tcatatttta tatttcagat gatttattaa tctaaaggcc atctgaaaac tccccctttc
     3541 atcaaatctt acaagccccg cccgcgcagc cgtcatcctg aatatcggtc tgcgtatcgt
     3601 ccgcaccgtc gcgggtgtta tggtagtaca gggttttgac gccgtatttg taggcggtca
     3661 gcaggtcttt gagcatttgt ttcatagaaa ctttgccgcc ttcgaatttg cccgggtcgt
     3721 aggcggtatt ggcggaaatc gattgatcga cgaatttttg catcacgccg acaagtttca
     3781 ggtagccttc gttgccggga agctgccaca gggtttcata ggcatttttc agggtttcaa
     3841 actccggcac gacttgtttc aaaatgccgt ctttcgatgc tttgaccgtt accaatccgc
     3901 gcggcggctc gatgccgttg gtggcgttgg cgatttgcga actggtttca gacggcatga
     3961 gcgcggtcag ggtggagtta cgcaggccgt atttgacgat gtcggcacgc aggctttccc
     4021 agtcgtaatg caaaggctcg ccgcagacgg catccaaatc ttttttgtaa gtgtcgatgg
     4081 gcagtttgcc ttgcgaataa acggtttggt taaagagcgt gcacgcaccg tattctttgg
     4141 caaggtttgc cgatgctttg agcaggtaat actgtatggc ttcaaaggta cggtgggtca
     4201 gaccgagcgc ggagccgtcg ctgtagcgga caccgttttt cgccagataa taagcatagt
     4261 taatcacgcc gatgccgagc gaacggcgac ccatagtaga ggtacgcgcg gcttctaccg
     4321 gatatccctg ataatctaaa agtgcatcga gcgcacgcac ggtcaagtcg gcaagccctt
     4381 ccaattcgtc caagctgttt aatgcgccca agttaaaggc agacagtgta cacagggcga
     4441 tttcgccgtt cggatcgttg atattgtcca gcggtttggt cggcagggcg atttccatac
     4501 acaagttgga ctgatgaaca ggcgcgacgc acggatcgaa cgggctgtgc gtattgcagt
     4561 gatcgacgtt ttgaatgtag atgcgcccgg tcccggcacg ctcctgcatc agcgtggaaa
     4621 acaggtcggc agccggaatg atacgcttgc gtatgttcgg gtcttgctcg tatttcgtat
     4681 agagccgctc aaattcgtct tggtcggcaa aaaacgcttc gtacaacccc ggaacctcgt
     4741 tgggcgaaaa cagcgtaatg ttgccgccct taatcaggcg ggtgtacagc aggcggttga
     4801 tttgcacgcc gtaatcaagc tgacggatac ggttgtcttc cacaccgcgg ttgtttttca
     4861 acaccagcag gctttcggct tcgatatgcc acaaggggta gaacaaagtt gccgctccgc
     4921 cgcgcacgcc gccttgcgaa caggatttga ccgccgcctg aaacattttg aaaaacggaa
     4981 tgcagccggt atgccgcgct tcgccgcccc ggatttcgct gtccaaaccg cggatacgtc
     5041 cggcattgat gccgatgccc gcacgctggg aaacgtattt cacaatcgcg ctggtggtgg
     5101 cgttgatgga atccaggctg tcgtcgcatt cgatcagtac gcagcttgag aactggcgcg
     5161 taggcgtacg cacgccgctc ataatcgggg tcggcagcga tactttaaat gtggaaacgg
     5221 catcgtaaaa ccgtttgacg taatcgaggc gtgtttcttt gggatatttg ctgaaaagac
     5281 acatcgccac cagaacatat aagaactgcg gtgtttcata aatctggctg gtaacgcggt
     5341 tctgtaccag atatttgcct tcgagctgtt tgacagcggc ataggaaaag gacatatcgc
     5401 gttcgtggtc gatataggcg ttcagttcat caaattcttc gcggctgtaa tcctcaagga
     5461 tatgcctgtc gtattttccg gcatcggtga gctttttaac gtggtcgtaa aggtgcggcg
     5521 gctcgtattc gccgtaggct attttacgca gatggaaaat cgccaaacgc gcggcaaggt
     5581 attggtagtc cggagtatct tccgaaatta aatcggcagc ggccttgatg atggtttcgt
     5641 ggatgtcgtc ggtgcggatg ccgttgtaga actggatgtg cgatttcaac tcgacctgcg
     5701 acacggaaac attttccaat ccttccgccg cccaagtaac gacacggtga atcttatcca
     5761 aatcaatggc ttctaatctt ccgtctcgtt tggttacttt caaatcagtc ggtgtattca
     5821 tcgcttcctc ttccactctt gatattcaag acacggtctt ttcaaacaaa ttaagacaga
     5881 caatatagtg gatttttggc attttctcca gtcttgacaa cggttgcatt tttcagtttc
     5941 aggcaatgcg cgataaaacc cgcctgtcgt atttttccta taatatttgt tttatctgat
     6001 aattctttac cgataaaaaa cgggtaaatt ttttgccttt tgaccggctc cggctacaag
     6061 gcggtgaaat aaggattttc cgacgaaaaa ggaaagcttc cggttttctg cccctgcgaa
     6121 ttgttaaatt ttgcaaagta tgattttgcc gcgccgccgc cgacaaattc cattttctta
     6181 ccgattgaaa tttattattg agattaatgt gttatttgaa tccgcatatc aaacggcaag
     6241 ttttgtcggc tgaatgagtc tgaaactgcc gacaatttgc ccgttccatc cttccatcct
     6301 atactggaag gaatgacaaa ctgaaaggat cgtcatatct gtcaaaaaga tgcttgccat
     6361 actgttgtct gcaatattgg gactggtatc gacaactgcc gctgccggta cgtcagaacc
     6421 cgcccaccgc cataccaaac atatccgcaa ggcaaacaag cagatgctgc accccgaatg
     6481 caggaaatat ttggaacgcc gtgccgcgtg gtaccgatcg caaggcaacg tgcaggaatt
     6541 gcgcgaaaac aaaaaggcgc gcaaagcatt ccgctccctg ccttatgcgg aacagaaaat
     6601 ccaatgccgg gcggcttatg aggctttcga tgatttcgac ggcggcagtt tccgccgtta
     6661 atcccatata aaatatg
//
LOCUS       DAWFBO010000107         6659 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFBO010000107 DAWFBO010000000
VERSION     DAWFBO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.107"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            730..2178
                     /gene="gnd"
                     /locus_tag="WGO87_001513"
     CDS             730..2178
                     /gene="gnd"
                     /locus_tag="WGO87_001513"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0392859.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFIEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2241..3512
                     /gene="waaA"
                     /locus_tag="WGO87_001514"
     CDS             2241..3512
                     /gene="waaA"
                     /locus_tag="WGO87_001514"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0392860.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRGERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGVLMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3553..4002
                     /locus_tag="WGO87_001515"
     CDS             3553..4002
                     /locus_tag="WGO87_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0392861.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            4035..5009
                     /locus_tag="WGO87_001516"
     CDS             4035..5009
                     /locus_tag="WGO87_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0392862.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFVFLKYGLA
                     FVLGFIGVKMLIMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            5078..5153
                     /locus_tag="WGO87_001517"
     tRNA            5078..5153
                     /locus_tag="WGO87_001517"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5111..5113,aa:Lys,seq:ttt)
     gene            5372..6625
                     /gene="murA"
                     /locus_tag="WGO87_001518"
     CDS             5372..6625
                     /gene="murA"
                     /locus_tag="WGO87_001518"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0392863.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVVEGA
                     GELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
BASE COUNT         1525 a         1619 c         1937 g         1578 t
ORIGIN      
        1 gcgggaatga cggcagagcg gtttctgttt tttccgataa atgccgcaat ctcaaatccc
       61 gtcattccca cgaaagtggg aatgacggaa tgtaggttcg tgggaatgac gcggtacagg
      121 tttccgttac gtatccccat ccgaaagttt atttatacac aagcaatcca aagttatctg
      181 actgaaatat tgtgagattt acccgcaaat ctcatttttt ccaccactta tacacaaatt
      241 cggatgcgca ctctgccgat ttgcagaaat aagacattcg tgccgacacc tgaccatttc
      301 cacaagttcc aaacacgctt ccggcttaaa tctccacagc attcccgttc ttaaaggcac
      361 cggcagccat tcggtaaaga aaagtaggat gccatctgca caacaagcaa actatccaca
      421 aaacttattc cccataaaaa acaatccaca ggtatgcaca aaagggaatc cgccttaccg
      481 acaccctaaa aaaaggtaat cctgatgatt tttaaaataa aaaaaagtta cccacagaaa
      541 atttctccta tcaatataat catcgttttt ttatttaatt tttatatttt atttataagc
      601 cttcagggaa acgcacggta aagcgtaaag gcatcttttc aaaaaattaa aaagttaagg
      661 attgttaaac aagtgtaaat caggtagagt tgcggtcgat aaattaaatc tataacggag
      721 cttcctgcaa tgaaaggcga tattggcgta atcggtttgg cggttatggg gcaaaacctg
      781 attttgaata tgaacgattg cggatttaag gtggttgcct acaaccgaac aatcggtaag
      841 gtggacgaat ttttaaacgg cgcggctaag ggaacggata ttgtcggcgc gtattccctg
      901 caagacttgg tcgataagct ggcaaaaccg agaaaaatca tgatgatggt tcgcgcaggt
      961 tcggtcgtcg acgactttat cgaacaactg cttccgttgt tggaagaagg cgacatcatt
     1021 atcgacggcg gcaatgccaa ttaccccgac acgacacggc ggacacatta ccttgccgaa
     1081 aaagggattt tgtttgtcgg ggcgggcgta tccggcggcg aagaaggcgc gcggcacggc
     1141 ccgtccatta tgccgggcgg ggataagcgc gcttgggaag ccgtcaaacc gatttttcag
     1201 gcgattgccg ccaaaacgcc gcagggcgaa ccgtgttgtg actgggtcgg cagggacggc
     1261 gcggggcatt ttgtcaaaat ggtgcataac ggcatcgaat acggcgatat gcagttgatt
     1321 tgcgaagcgt accagtttat gaaagacggc ttggggctgt cttacgacga aatgcaccgc
     1381 gtgtttgccg agtggaacaa aaccgagctg gattcttatc tgattgaaat cactgcggca
     1441 attttgggct ataaggacga aggcggcgaa ccgctggtcg agaaaatcct cgatacggca
     1501 gggcaaaaag gcacgggcaa atggacgggc atcaatgctt tggatttggg tattccgctg
     1561 acgctgattt ccgaagcggt attcgcccgc tgcgtttcgt cgttcataga acagcgcgtg
     1621 cagaccggca agctgttcgc ccgaaccgtt acgcccgtcg aaggcggcaa acaagaatgg
     1681 gtcgaggcgt tgagacaggc cctgcttgca tctaaaatca tttcctacgc acaaggcttt
     1741 atgctgatcc gcgaagcggg cgaaagctac ggctgggatt tggactacgg caacactgcg
     1801 ctgctgtggc gcgaggggtg catcattcgc agcgcattct taagcaatat ccgcgacgcg
     1861 tatgaaaaca atcccgattt ggtgttcttg ggtgcggatc cgtatttcaa aaatattttg
     1921 gaaaactgcc tgccggcgtg gcgcaaggtg gttgccaagg cggtcgaatg cggcatccct
     1981 atgccctgca tggcatcggc gattacgttc ttggacggct acacgaccga acgtctgcct
     2041 gccaacctct tgcaggcaca gcgcgactac ttcggcgcgc acacgtacga acgcaccgac
     2101 aaaccgcgcg gcgagttttt ccataccaac tggacgggca agggcggaga taccgcttcg
     2161 acaacctacg atatttgatt ccgttgccga aatgccgtcc gaaccgttcg gacggcattt
     2221 gttttgcagg aaatagaaag atgttccaat ggctttatga tgtattgtgg ctgcttgcgc
     2281 cgatatggat acggcgttat ttggacaaac gctccggaag tgccccggca tatcgggcgc
     2341 atcggggcga gcgtttcggc aagccgtatc cgaatcccgt taccggcgcg gtttggatac
     2401 acgccgtttc ggtcggggaa acgcgtgccg cccaaccttt gatacgcgag ttgcggcagc
     2461 gttttcccga tgcgccgctg ctgatgacgc agatgacccc gacgggacgg gaaaccgcgc
     2521 aagttctgtt tcccgatgcg caatgccgct atcttccgta tgacaaaaaa acgtgggtac
     2581 ggcagttttt gcgcgaacac cgcccgatgt tcggcgtatt gatggaaacg gaaatctggc
     2641 ccaacctaat gaaggaatgc cggcgcgcgg gtgtgccgct gtttttggcg aatgcgcggc
     2701 tgtcggaaaa atcgttgaac ggttatctga aagtccgccg cctgatccgt cctgccgccg
     2761 cttcgctgac gggttgtctg gcgcagacgg aggcggatgc ggcgcggttg gcgaaattgg
     2821 gcgcggcatc cgtgcaggtg tgcggcaata ccaaatacga cctgatgccg tcggaacaga
     2881 tgaaaacgct ggcggggcag tttgaaaaac gcatcggagg ccggccggtt gccgtgtgcg
     2941 gcagcacgcg cgtttatcgg ggcgaagacg aggcggaaaa actgctggcg gcgtggcaac
     3001 aatatcgcgg cgatgcgctg ctggtcgtcg tgccgcgcca tcccgagcat tttcagacgg
     3061 tatttgaaac ggcaaaacgc ttcgggttta aggttcagcg gcgcagcgac ggtttgccgg
     3121 tcgaacctga tacgcaggtg tggataggcg acagtatggg cgagctgtat gcgtattacc
     3181 tgtgcgccga tgttgctttt gtcggcggca gtctggtcga ttcgggctgt cagaacatca
     3241 tcgaaccgct ttcctgcggc gttccgacga tattcggctt ttcgacctac aatttttccg
     3301 aagcctgccg gcacgccttg gcatcgggtg cggcggttca agtcgaatcg gcggatgcgt
     3361 ggcgtgaagc cgttgaaaaa accttatcgt ccgagggggg cggaatgcag atgcaggcgc
     3421 gcgtggacgg ctttatcgca caacatcgcg gagcgggcgc gagaatcgcc gaggcggtgc
     3481 gggaagcggt atgcggacat cgggggcgat agtgatacaa tccgtatccg agttttccgg
     3541 ttggagtacc gtatgagtat ttatgccgtc gcgcacatcg tccacctgta ttgcgccatt
     3601 gcctttgtcg gcggcgtgtt ttttgaagtg ctggttttgt ccgtcctgca tacgggacgg
     3661 gtgtcgcgcg aggcgcggcg cgaagtggaa aaggcaatgt cttaccgcgc cgtcagggtg
     3721 atgccgtttg tggtcggact gctgttcgcc agcggcatcg tgatggcggc aaaccgctat
     3781 ctttctatat tgggcgaacc gtttgccact tccttcggta cgatgctgac gctgaaaatc
     3841 ctgttggcgt tcagcgtgtt ggcgcacttc gccatcgccg tcgtcaaaat ggcgcgttcc
     3901 acactgaccg tcggctggtc gaaatacata cacaccgtcg tctttaccca tatgctgctg
     3961 attgtctttt tggcaaaagc gatgttttat atcagctggt aacacaacta catttcaaat
     4021 caggagattg aaccatgaca gaatatcccg gcatcgggtc gccgttgttt tacggcgttt
     4081 tttttgcggc agtgctggtc atgattgcct tggatatgtt ttcgctgaag aaaaacagca
     4141 gccataaagt cggcgtcaaa gaagccttgg catggagcgg cttgtgggtt gccgtatcct
     4201 gcctgttcgc aggctggctg tatttcaaac tcgccggcaa tcccggctac ggcgcggcag
     4261 tcgcaaaaga aaaagtattg gaattcttta ccggctacat tttggaaaaa tcgttggcag
     4321 tcgacaatat tttcgtgttc ctgatgatat tcggctactt caaagtcgca ccgcagtttc
     4381 agcaccgcgt gctgctgtac ggcgtattgg gcgcattggt attgcgcact gtcatgattt
     4441 tcgtcggcgc ggcactggtt cagcagttcg agtggattct gtatctgttc ggcgcgttcc
     4501 tgctctatac cggcatacac atgatgaagc ccgaaggcga tgaggaaggg gatttggcaa
     4561 acagcaggct gctgaatgcc gtcaagaaag tcgttccggt cggcacggaa tttcacggcg
     4621 agaaattttt tactgtcgaa aacggtaaaa aaatcgccac gccgctgttt ttggtgctgg
     4681 tcatgattga attgagcgat gtcgtttttg ccgtggacag catccccgcc gtctttgccg
     4741 ttaccaccga tccgtttatc gtgctgactt ccaatatttt cgcaattctg ggcttgcggg
     4801 cgatgtattt cctgctggcg gatgtggcgg aacgcttcgt attcctgaaa tacggcttgg
     4861 cattcgtgtt gggttttatc ggtgtgaaaa tgctgataat gcattgggtg catatcccga
     4921 tttccgtttc gctgtcggtc gtgttcggcg cattgggtgc atcggtgttg acttcattgg
     4981 tttatacgaa aaaacagtcc gataaataaa atgccgtctc aacgccgaca aaatggttga
     5041 cagcatcagg cttgatgtat agaatttcga gcttgtcggg tcgttagctc agtcggtaga
     5101 gcagcggact tttaatccgt tggtcgagcg ttcgaatcgc tcacgaccca ccagataaaa
     5161 caaaaaagct gagttttcaa aactcggctt tttttgtttt ccgacaatcc ttgttcggaa
     5221 agcgcggcat ttcgtcaaat tctttggtgc ggcttccgta tttttcccga aaatccctta
     5281 taattccgcc tctgcaacct tcatcgggtg atgctttatg cgttgtcccg agtattgaaa
     5341 tattacaagt acccaccagg aaagaacaaa cgtggacaaa ctgaaaatct ccgcaaacgg
     5401 cccgctcaac ggggaaataa cggtctcggg cgcgaaaaac gcggcattgc cgctgatgtg
     5461 cgcgggtttg ctgacatcgg gtacgttgcg cctgaaaaac gtccctatgc tggcagatgt
     5521 gaaaaccacg caaaagctgc ttcaggggat gggcgcgcgc gtcctgactg acaatatcag
     5581 cgaatttgaa atcaacggcg gtacggtaaa caatacctgc gccccttacg aattggtacg
     5641 gacgatgcgg gcctcgattt tggtgctggg gcctactttg gcacgtttcg gcgaggcgca
     5701 agtcagcctg ccgggcggct gcgccatcgg ttcgcgcccc gtcgatcagc atttgaaagg
     5761 cttggaagcg atgggtgctg agattgttat cgaacacggt tacgtcaaag ccaaaggcaa
     5821 actcaaaggt acgcgcgtgg cgatggatgt cgttaccgtc ggcggcacgg aaaacctgct
     5881 gatggcggcg acgctggcgg aaggtacgac ggttttggaa aactgcgcca ttgagcctga
     5941 agtggtcgat ttggcggaat gcctggtcaa aatgggcgcg aaaatcagcg gcatcggtac
     6001 gtccacgatg gttgtggaag gcgcgggcga gctacacggc tgcgaacaca gcgtcgtccc
     6061 cgaccggatt gaagcgggga cgtttctgtg cgcggtggcg ataaccggcg gcagggtggt
     6121 tttgcggaat gccgccccga aaacgatgga agtggtgttg gacaaactgg ttgaggcagg
     6181 tgcggtgatt gaggcgggcg atgattggat cgccatcgat atgcggcagc gtccgaaggc
     6241 ggtggacatc cgcacggtcg tccaccccgg cttccccacg gatatgcagg cgcagtttat
     6301 ggcattgaat gccgtggcgg agggaagctg ccgcgtggtg gaaacgattt ttgaaaaccg
     6361 ctttatgcac gtccccgagt tgaaccggat gggggcgaac attacaaccg agggcaatac
     6421 ggcatttgtg cagggtgtgg aacggctttc cggcgcagtc gtcaaggcga cggatttgcg
     6481 tgcgtccgca agcctcgtta tcgccggttt ggcggcgcgc ggcgaaaccg tggtcgaaca
     6541 gatttaccac ttggatcgcg gttatgaaaa tattgaaaaa aaactcggca gcgtcggcgc
     6601 gaaaatcgag cgcgtatccg gctgatcggg ttgcgatgcc gtctgaagcc ttcagacgg
//
LOCUS       DAWFBO010000108         6469 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFBO010000108 DAWFBO010000000
VERSION     DAWFBO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.108"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(141..1529)
                     /locus_tag="WGO87_001519"
     CDS             complement(141..1529)
                     /locus_tag="WGO87_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0392864.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLIDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            1857..2804
                     /locus_tag="WGO87_001520"
     CDS             1857..2804
                     /locus_tag="WGO87_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0392865.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="WGO87_001521"
     CDS             complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="WGO87_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0392866.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            3487..3960
                     /locus_tag="WGO87_001522"
     CDS             3487..3960
                     /locus_tag="WGO87_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0392867.1"
                     /translation="MTDFSVWETAPFGATVDYILQRYNNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLAS"
     gene            complement(4030..4851)
                     /gene="nadE"
                     /locus_tag="WGO87_001523"
     CDS             complement(4030..4851)
                     /gene="nadE"
                     /locus_tag="WGO87_001523"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0392868.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTADLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            5048..6403
                     /locus_tag="WGO87_001524"
     CDS             5048..6403
                     /locus_tag="WGO87_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0392869.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTAVQGAGSEFQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASISKLAQTLSYSMTQNVRT
                     HTARFERQTQALQHYRPDISVCKKNIDRFQTALPAAFSQLLARRRQSLSAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT         1470 a         1708 c         1743 g         1548 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatattgc agattaatcc tttgggtatc aaagccgggc
       61 ggtgttgaaa cattgcaaaa atgccgtctg aaactcgaaa tcggtttcag acggcattgg
      121 tttatgaggc aggttggggc ttagcggtct ttgtaacggc gttcgctttt gcctgcaaaa
      181 gaatcgccgg ctttacgttt gcctttgaag ccttcgccgg tttttttgaa accgtctttc
      241 ttaaagcctt ctttcttgaa accttcgccg cgcgttttgc cgccgaagcc ttctttgccc
      301 ggtttatgat cgccgcgcca accgccggat ttacgatcgc cccagccgcc tttgcctttc
      361 ggcttgccgc ctgcggattt gcgtttgcgg gtcggttcca tgccctcgat ggtcagttcg
      421 ggcagttttc ggttaatgta tttttcgatt ttgtggactt tgacgtattc gttcacttcg
      481 gcaaacgtaa tcgcaatacc cgtgcggcct gcgcggccgg tgcgcccgat gcggtggacg
      541 tagtcttccg cctgtttcgg caggtcgtag ttgataacgt gggtaatggt cggtacgtcg
      601 ataccgcgtg cggcgacgtc ggtggcaacc aaaattttgc agcggccttt acgcaaatcc
      661 atcagtgtgc ggttgcgcca gccttgcggc atatcgccgt gcaggcagtt ggcggcgaaa
      721 cctttttcgt acagttcatc cgcaatgact tcggtcatgg ctttggtgga cgtgaaaatc
      781 acgcattgat cgatgtcggc atcgcgcagg atgtggtcga tcaggcggtt tttgtgacgc
      841 atatcgtcgc agtacagcag ttgttcttcg attttgcctt ggtcgtccac gcgttcgact
      901 tcgatgattt cagggtcttt ggtcagtttg cgtgccagtt tgccgaccgc gccgtcccaa
      961 gtggcggaga acaataaagt ctgacggtcg ctcggcgttg cttccacgat ggtttcgatg
     1021 tcgtcgataa agcccatatc caacatacgg tcggcttcgt ccagaatcag cacttccaaa
     1081 cgttcaaaat cgactttgcc gctttgcatc aagtccatca ggcggcccgg cgtggcgaca
     1141 atcagatcga ccggtttgct cagggcgcgg gtttggtagc cgaacgatgc gccgccgacg
     1201 atgctgacgg tacggaacca acgcatattt ttggcatacg ccagcgcgtt tttctcgact
     1261 tgagccgcca attcgcgggt cggggtcaac accaaagcac gcgggccttt gcccggtttt
     1321 tcgctgcgtt tggtcagtcg ctgcaaagtc ggtaacagaa aggcggcggt tttgccggag
     1381 ccggtttgcg ccgaagccat gatgtcgcgg ccttccaaag caaacggaat cgcctgcgcc
     1441 tgaatcggcg tcgggctttc gtaaccctcg ctgctgacgg cggacaaaat gtttttatca
     1501 aggttcaaat cggcaaattt aatagacatg gctatccttg gaagacaaca acgcccgcgt
     1561 ccgaaaggtt cagacggcct gaagcaggga agggtaacga tacggggaat gtgaagttac
     1621 agggttgtcg caagcggctt gcttgtggtt tacatcgacg gcaacctgca cagatacgct
     1681 ttgcaaaatc aaagcaacag aagaaacgcg cccgtaaggg cggtttaggt tggaaggcga
     1741 tggcttcgta tgacaagata agcggtggat ggtatagggt ttgcccgaaa cggtcaagca
     1801 atcgggcgtt aattacagta aaattgccgt tttccacttt attccgcctg aaaccaatga
     1861 ccgacatcac accgtttgcc aaccgcttgg gcaaaaacat caaacacctt atgaaatggg
     1921 cgaaacgcaa cggcatcgaa gcctggcgca tttacgaccg cgacatcccc caattcccct
     1981 ttgccgccga tgtttacggc gatcgaatcc accttcagga atacgatacc ggctggctga
     2041 tgcggcccga agaatacgaa gcgtggcttg ccgaagtctt ggaagccgtc gccttcgtta
     2101 ccggttttgc gcccgaacaa atccgcctca aacgccgcga acgccaaaaa ggtttgcagc
     2161 aatacgaaaa aaccggcaag gcgggcgacg atttcgtcat caccgaaaac ggccgcaagt
     2221 tttgggtcaa ccttgataag tatttggata cggggctttt tttggatcat cgaaacacgc
     2281 gcaaaaaagt cggcgaaacg gcggcgggca aacgtttttt aaacctgttt tcctacaccg
     2341 gcagcttcac tgtctatgcc gccaccggcg gcgcggcatc cagcgaaact gtcgatttgt
     2401 ctaacactta tctcgattgg gcgagacgca attttgaatt gaacggcatc gatacggaac
     2461 ggcataaaat cgtccgcgcc gacgtgttcc aatatcttca gacggcatat ggggaaggca
     2521 ggcggttcga cctcattgtg atggacccgc ccagtttttc caacagcaag aaaatgtccg
     2581 acatcctcga catccagcgc gaccacaaaa agctgattga cggcgcggtg aagctgctcg
     2641 cttcagacgg cattttgtac ttttccaaca acctgcgcag cttcgtctta gacgacttgg
     2701 tatcggaaca atacgccgtc aaagacattt ccaaacaatc cgttcccgaa gatttccgca
     2761 acaaaaaaat ccataggtgc tgggaaatcc gccacaaatc ttaagggtgc gaaaaagccg
     2821 ccggaatctt ccggcggctt tttcgattgc gctttatcag gcgatagagg cttcgacaaa
     2881 ggcggtcagc tgaccttttg ccaatgcgcc gactttggtg gcgacgactt cgccgttttt
     2941 gaacaccatc agggtcggaa tgccgcgcac gccgaaacgg gacggggtgg cttcgttgtc
     3001 gtcgatgttg attttgacca ctttcagacg gccttcaaat tcggcggcaa tgtcgtccaa
     3061 aatcggggca atcattttgc aggggccgca ccacggagcc caaaagtcca gcaggacggg
     3121 gatatctgca tttaaaacgt ctttttcaaa agcggcatcg ctggtgtgta caatcaattc
     3181 gctgctcatt atttttcctt ttcggttggg attggcacga tagcgtgcag gatagggctt
     3241 gggcataaaa atttcaagtg ggcgggcggg ataatagttt ttctgtaccg ccccaatcgc
     3301 ctttgcaaat tctaaaaaac ggttcggaca gtattttatt tttgaaaatt acttttatta
     3361 tcaaagtgta aggaatgttt ttgaagctgt acagaaacgc gcgggcggtg tatgctccgt
     3421 ttaaatagat tcttaaagta tgaatgttca aaaccaaacc cgaaacccta taaaaggaac
     3481 actgcaatga ctgatttttc cgtttgggaa acagccccct ttggcgcaac cgtcgattat
     3541 atcctgcaac gctacaacaa tgtccaccgc gcacaatttg aagaattggt gccgctggcg
     3601 cagaaagtgg cccaagtcca tgccgatacc tttccggcgg aaattgccgg gctgcttgcc
     3661 tatatgcaga acgaactgct gatgcatatg atgaaagagg aaaggatgct gtttccgatg
     3721 attaatcagg gtgtcggacg cggtgcggca atgcccatca gcgtgatgat gcacgaacac
     3781 gaagaacacg accgcgccat cgcacggctc aaagagctga ccgacaattt ccaaccgccc
     3841 gaaggggctt gcggaagctg gacgcggctt tacgcgctgg cgaaagaaat ggcggacgat
     3901 ttgaacgacc acattcattt ggaaaacgat attctgtttg cccgcgtact tgcttcttaa
     3961 ggaacggaag ccggatatgt caatgccgtc tgaaacacgg ttttccgctt cagacggcat
     4021 tttcatgttt cagcccaaca attcgggcgg aatgcggcac acgggaatcg ggttgatttt
     4081 gtgctgcatg gcgcggtgaa gtcgcgtata gatttctaga acttcgcgct gccgcccttc
     4141 aaaatcttcg ggtttgcgcg tgccgtacac gcccattgcc cactccagtt cgggatagct
     4201 tgcgcccatc tgttcttcgt cggtgcgttc cgtatcccac aggccgtcgg tcggcggggc
     4261 tttttgaatc gcctcgtcca cgcccaatgc ttcggcaagc cggtaaacct gcgttttggt
     4321 caggtcggca atcgggctga tgtccacgcc gccgtcgccg tatttggtaa aaaatcccac
     4381 gccgaaatct tcaattttat tacctgtccc cgtaaccagc agtccgtgta tctgcccgta
     4441 gtagtacagg gtcagcatac gcaggcggct tctggcgttg gcgagggaaa gcggctgatt
     4501 gtcaaatgcc gtctgatgaa tgccgacggt ttgttcaaag gtctggaagg tgtcggtcaa
     4561 atcggccgtt tgcgcgctta cattggcata ttgccgttgc agattgcgga tgtgcagcct
     4621 tgcccgctca agctggtcgg ggtgttggcg tatcggcata tccagaagca gcgtggtgcg
     4681 gccggtgcgg gcggcgagtg cggagacgac ggcggaatcg atgccgccgg aaacgcctac
     4741 gacgaaccct tttgcatttg cccgggcggc gtattcgtcc aaccatcggg cgatatgtgt
     4801 gataactgcc tgcgtatcca tttttcctcc tgtcgaaaat atccgcatca tagcctgccg
     4861 gcggtgcaat ttgaatagat tgcaggcagg ttgatgaata tttttcccat tccccgctta
     4921 aaaaagtcat acgggaaaag gcgggcggca agatggcgcg tgttataata ttgttgacaa
     4981 tttaggcggc ccgatgccgc cttgggcttc aaaacatatt catatttttt attatttgat
     5041 tgattcgatg tctgattttt ttcattctga cgtgttgtcc gtttccgagt tgaacgcatt
     5101 tgccaaaagt attttggaaa accaccttgc cggtttgtgg attgccggcg aagtgtccaa
     5161 cctgacccgc gccgccagcg gacattatta cttctcgctt aaagacagcc gcgcgcaggt
     5221 gcgttgcgcg atgttcaaag gtgcggcggc gcgtttggcg aagcctttga aagaaggcga
     5281 ccatatcgaa gtatcaggga aaatcagtat ttatgaaacg cggggcgaat ttcagattac
     5341 cgtgaacgaa gtgcggctca agggcttggg gcagctttac gaagcctatg agcggttgaa
     5401 ggcgcagttg caggcggaag gcgcgtttgc ggcggaacgc aaaaaacctc tgcctgcccg
     5461 accgcaatgt atcggcatcg taaccagttt ggcggcggcg gcattgcgcg atgttgtaac
     5521 caccttgaaa cgccgcgcgc ccgaaattcc cgtcatcgtt tatccgactg ccgttcaggg
     5581 cgcgggcagc gagtttcaga ttgcccaagc gattaaaacc gcatcgcaac gcgccgaatg
     5641 cgacgtgttg attgtctgtc gcggcggcgg cagcattgaa gatttgtggg cgtttaacga
     5701 agaaccggtc gtacgcgcca ttgaagcctg cacgattccg gtcgtcagcg gcgtaggcca
     5761 cgaaaccgat ttcacgcttg ccgattttgt tgccgacgta cgcgcaccga caccgaccgg
     5821 cgcggcggag ctggtcagtc ccaaccgcca agaatcgtta catcgtctag cccaagcgca
     5881 aggccgtctg aaaactgttt tggagcaacg ctatttcgat gccagccaaa aactcgactg
     5941 gctcgcacgg caaatccgtc atccgcgcca aaaacttgac gagcaacgcg cttcaatcag
     6001 caaactggcg caaacgctgt cttactcgat gacgcaaaac gtccgtaccc acaccgcccg
     6061 ttttgaacgc caaacccaag ccctgcaaca ttaccgtcct gatatttccg tttgcaaaaa
     6121 aaacatcgac cgctttcaga cggcattgcc cgccgccttc agccaattgc tcgcccgccg
     6181 ccgccaaagc ctgagcgccc aagcagcatt gctcgaagcc gtttcgccgc agcacattct
     6241 ggagcgcggc ttttccgtcg tcaaaaacac acgcggacaa gtcatccgca acgccgatgt
     6301 tttgaagcag gggcagaagc tgcacattat cttcgccgac ggcgaaaccg acgtgcgcgt
     6361 aagcaaagaa caagggcagc aggatttgtt cgattgcatt taaacccaat cggaaaaacc
     6421 gctccccctc cgccgtcatt cccgcgaaag cgggaatcca gtcccttga
//
LOCUS       DAWFBO010000109         6441 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFBO010000109 DAWFBO010000000
VERSION     DAWFBO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.109"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            116..1657
                     /locus_tag="WGO87_001525"
     CDS             116..1657
                     /locus_tag="WGO87_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0392870.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGWQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRACSDKAVSPVMPSEPQSVGEYVLL
                     AFAAAVLGICCLFPLAAVVVKACSAGESWQVLAERETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTAYRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     QTETL"
     gene            1686..2285
                     /locus_tag="WGO87_001526"
     CDS             1686..2285
                     /locus_tag="WGO87_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0392871.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVELIRRHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2282..2887
                     /gene="nadD"
                     /locus_tag="WGO87_001527"
     CDS             2282..2887
                     /gene="nadD"
                     /locus_tag="WGO87_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0392872.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            2946..3332
                     /gene="rsfS"
                     /locus_tag="WGO87_001528"
     CDS             2946..3332
                     /gene="rsfS"
                     /locus_tag="WGO87_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0392873.1"
                     /translation="MNEQELQDLQKMVEVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3386..3856
                     /gene="rlmH"
                     /locus_tag="WGO87_001529"
     CDS             3386..3856
                     /gene="rlmH"
                     /locus_tag="WGO87_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0392874.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRIFEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFI
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            3931..4197
                     /locus_tag="WGO87_001530"
     CDS             3931..4197
                     /locus_tag="WGO87_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0392875.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4354..5037
                     /locus_tag="WGO87_001531"
     CDS             4354..5037
                     /locus_tag="WGO87_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0392876.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5161..5673
                     /gene="coaD"
                     /locus_tag="WGO87_001532"
     CDS             5161..5673
                     /gene="coaD"
                     /locus_tag="WGO87_001532"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0392877.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5705..6358
                     /locus_tag="WGO87_001533"
     CDS             5705..6358
                     /locus_tag="WGO87_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0392878.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1412 a         1699 c         1849 g         1481 t
ORIGIN      
        1 ctatatttca gacggcggga ggaaggcgga tacggtatgt agtcggattg aaatatacgg
       61 tatgtggggg cttttgggta caatcgcaac atcagtgttt caagtgggga aggcgatgga
      121 tggacggcgt tgggtggtat ggggtgcttt tgccctgctg ccttcggctt ttttggcggt
      181 aatggtcgtt gcgcctttgt gggcggtggc ggcgtatgac ggtttggcgt ggcgcgcggt
      241 gctgtcggat gcctatatgc tcaaacgttt ggcgtggacg gtatttcagg cagcggcaac
      301 ctgtgtgctg gtgctgcctt tgggcgtgcc tgtcgcgtgg gtgctggcgc ggctggcgtt
      361 tccggggcgg gctttggtgc tgcgcctgct gatgctgccg tttgtgatgc ccacgttggt
      421 ggcgggcgtg ggcgtgctgg ctctgttcgg ggcggacggt ctgttgtggc gcggctggca
      481 ggatacgccg tatctgttgt tgtacggcaa tgtgtttttt aacctgcccg tgctggtcag
      541 ggcggcgtat caggggtttg tgcaagtgcc tgcggcacgg cttcagacgg cacggacgtt
      601 gggcgcgggg gcgtggcggc ggttttggga cattgaaatg cccgttttgc gcccgtggct
      661 tgccggcggc gtgtgccttg tctttttgta ttgtttttcc gggttcgggc tggcattgct
      721 gctgggcggc agccgttatg ccacagtcga agtggaaatt taccagttgg tcatgttcga
      781 actcgatatg gcggttgctt cggtgctggt gtggctggtg ttgggggtaa cggcggcggc
      841 agggttgctg tatgcgtggt tcggcagacg cgcgtgttcg gataaggcag tttctcctgt
      901 gatgccgtct gaaccgcagt cggtcgggga atatgtgctg ctggcgtttg cggcggcggt
      961 gttgggcata tgctgcctgt tcccgctggc ggcggtcgtg gtaaaggcct gttctgcggg
     1021 agaatcttgg caggtgttgg cggaacgcga aacttggcag gcggtgtgga atactttgcg
     1081 cttctcggcg gcggcggtgt ttgcggcggc ggttttgggt gtggtgtatg cggcggcggc
     1141 gcggcggttg gcgtggatgc gcgggctgat gtttttgccg tttatggtat cgccggtttg
     1201 tgtttcggca ggcgtgctgc tgctttatcc gcagtggacg gcttcgttgc ccttgctgct
     1261 ggcgatgtat gcgctgctgg cgtatccgtt tgtggcaaaa gatgttttgt cggcctggga
     1321 cgcactgccg ccggattacg gcagggcggc ggcgggtttg ggcgcaaacg gctttcagac
     1381 ggcataccgc atcacgttcc cccttttgaa accggcgttg cggcgcggtc tgaccttggc
     1441 agcggcaacc tgcgtgggcg agtttgcggc gacattgttc ctgtcgcgtc cggaatggca
     1501 gacgttgacg actttgattt atgcctattt ggggcgtgcg ggtgaggata attatgcgcg
     1561 ggcaatggtg ttgacattgc tgttgtcggc atttgcggtg tgcattttcc tgctgttgga
     1621 caacggcgaa ggcggaaaac agacggaaac gttataatgt gaaccctttt tcagaggacg
     1681 gcaaaatgag cgagttggat aacatccttg cccataaccg gcagtttgtc gagtcgggcg
     1741 aatatgaaaa atactttacc gacaaatacc ccgaacgcgg gctggcagtt ttgtcctgta
     1801 tggatgcgcg gattatcggg ctgctgcccg acgcgttggg tttgaaaaac ggcgatgcca
     1861 agctgattaa aaatgccggc gcgctggtta cgcacccgtg gggttcggtg atgcggagtc
     1921 ttttggttgc cgtgtttgaa ttgaaggtca gagagattat ggtcatcgcc catcacgatt
     1981 gcggtatgca ggggctgaat gccgaagaat tcctcgggcg cgtccgggaa agccagattc
     2041 ccgaagaccg tatcgaaacc ctgcgttatg ccggtatcga cctcgacggc tggctgaccg
     2101 gtttcgacaa cgtcgaagac agcgtgcgcc atactgtcga actcatccgc cgccatccgc
     2161 tgatgccgcg ccatattgcc gttcacggac tggtcatcca tcccgttacc ggcaaactga
     2221 cgctggttgt ggacggcagt gtttcagacg gcatggactt atcggaagga atggaaacat
     2281 catgaagaaa atcggactgt tcggcggcac tttcgacccg atacacaacg ggcatctcca
     2341 catcgcccgc gcctttgccg acgaaatcgg cttggacgcg gttgttttcc tgccggcagg
     2401 cggcccgtat cacaaagacg cagcctccgc ttccgccgcc gaccgccttg ccatggtcga
     2461 actggcgacg gcagaagacg cgcgttttgc cgtcagcgat tgcgacatcg tccgagaagg
     2521 tgcaacctat acttttgata ccgtccaaat cttccgccag cagttcccat ccgcgcaact
     2581 ctggtggctg atgggcagcg acagcctgat gaagctgcac acatggaaaa aatggcagat
     2641 gctcgtgcgc gaaaccaata tcgccgtcgc catgaggcag ggcgacagcc tgcaccaaac
     2701 cccgcgcgaa ctgcacgcgt ggctgggcaa gtcccttcag gacggcagcg tccgcatctt
     2761 gtccgccccg atgcataatg tgtcgtcaac ggaaatccgc cgcaaccttg ccggccaagg
     2821 cgtttcagac ggcatcccgc ctgccgccgc acgctacatc cgcgaacacg gtttgtatga
     2881 aaaataaagt caaatcagta agaaacggct ataatgccgt ctgaaaacat cccgttagga
     2941 acataatgaa cgagcaagaa ctgcaagacc tgcaaaaaat ggtcgaggtc gccgtcaacg
     3001 ccctcgaaga catcaaagcc aaagatattt ccgttctcga aacgcaagac aaaacttcgc
     3061 tgtttgccag aatgattatc gccagcggcg acagtacgcg ccaagtcaaa gcccttgcca
     3121 acaacgttgc cgtcgatttg aaagaagccg gttttgaaat cctcagtacc gaaggcgaca
     3181 gcggcgaatg gacgttggtt gatgcaggag acctcgtcgt ccacgtcatg ctccctgccg
     3241 tgcgcgactt ctacgacatc gacaccctct ggggcggcga gaaaccgagt ttccacgccg
     3301 gaatgcagaa gccgtggcac gcggcagact gattcccgat gccgtctgaa tgttcagacg
     3361 gcattttcct gtaagggaga aagcattgaa cattaccgtt ttggcagtcg gcaccaaaat
     3421 gccgcgctgg gttgatgagg ccgtcgccga atacgccaaa cgcttcggac gcgacgtcgc
     3481 ctacgcactc aaagaaatca aacccgaaaa acgcggcgcg ggcgtgaatg ccgcccaagg
     3541 tatggcggcg gaagaaaaac gcatcttcga agccataccg caaggcgcgt tcctcgtcgt
     3601 tcttgacgaa cgcggcaaag caccgacctc cgtcgagctg gcggaacacc tcaaaagctg
     3661 gcagcaaaac ggcgaacacg tctgcttcat catcggcggc gcggacggca tgaccgaccg
     3721 cctcaaacaa caagcccgca tgatgatgcg cctgtccagc ctcaccctgc cgcacggcat
     3781 ggtgcgcgtc cttctgaccg agcagctcta ccgggctgtt tccatcctgc acaaccatcc
     3841 ttatcatcgg gaataagaag gcttttgtct gtacccctaa tcggttaaaa tcaccccgtt
     3901 atttctgaac acacagcaaa ggaatccgct atggcacgca tggtattctg cgtcaagctc
     3961 aacaaagaag ccgaaggcat gaaatttccg ccgcttccca acgaattggg caagcgcatt
     4021 tttgaaaacg tatcgcaaga agcgtgggcg gcgtggacgc gccaccaaac gatgctgatt
     4081 aacgaaaacc gtttaagcct cgccgatccg cgcgcgcgcg aatacctagc tcagcagatg
     4141 gagcagtatt tcttcggcga cggcgcggat gccgttcagg gatacgttcc gcaataacgg
     4201 ttttccgttt tgaacacagg ctgtccgaaa ctgcttcaga cggcctttaa aatacgccgg
     4261 acaactttat cttcacgcaa ccggacaaat tttgacattg ggcaatgtta aaatccccca
     4321 ttattcccaa ccgcgctttc aggagcagat gatatgcaac acgacgttta cgactacacc
     4381 gcgcatacgg tttctaaaaa caccgtcctg cagaaaacct accgcctgct cggattttca
     4441 ttcattccgg cagccgcagg cgcggcactt gccgccaatg ccggtttcaa tttttacgcc
     4501 gccttcggtt cgcgctggat agggtttgcc gtcgtgttgg cgtttttcta cggtatgatc
     4561 cacttcatcg agaaaaaccg ttacagcaat accggcgtta ccctgctgat ggtattcaca
     4621 ttcggtatgg gcgtattgat cggccccgtg ctgcaatacg cgctccatat tgccgacggt
     4681 gcgaaaatcg tcggcattgc cgccgcgatg accgccgccg tctttttaac gatgtccgcc
     4741 ttggcgcgcc gaacccggct cgatatgaac gcgctcggac gcttcctgac cgtaggtgcg
     4801 gtcattctga tggtcgccgt ggttgccaat ctgtttttgg gtattcccgc actcgccctg
     4861 accatttccg ccggttttgt cttgttcagt tccttaatga ttatgtggca ggtacgcacc
     4921 gtcatcgacg gcggcgaaga cagccacatc agcgcggcac tgacactgtt tatctcgctt
     4981 tacaacatct tcagcagcct gctgaacatc ctgttgtctt taaacggcga ggattgatgc
     5041 cggtaaaacg ccgtccgact atgccgtctg aaaatgcttc agacggcatt ttactttggg
     5101 catacaatac ggatgcacac gcaccaacac acagccgact atgaagccgg gaggaatcaa
     5161 atgttaccga atacaccgcg ccgcgccgtt tatgccggca gtttcgatcc gcccacattg
     5221 gggcatctgt ggatgatacg gcaggcgcaa tctatgtttg acgaactcat cgtcgccatc
     5281 ggcattaacc ccgacaaacg cagcacctat accgtcgctg aaaggcagga tatgttgtgc
     5341 gatattacta aaatgtttcc caacgtcaga accgatgtat ttgaaaaccg atttctggtg
     5401 cattacgccc gtgaggtaga tgcaggattc atcgtgcgcg gcatccgttc tgcttcggat
     5461 tacgaatacg aacgttccat gcgccatatc aacagcgacc tcgcccccga aatatccacc
     5521 gtattcctca tgccgccgcg cgaaatcgcc gaagtgtcgt ccactatggt caaaggactg
     5581 gtcgggccgg aaggctggac ggaaacgatc caccgctacg tgccgcaagc tgtgtacgaa
     5641 aaaatccttg ccgaacatca acacgaaaat tgaacccttc tatttttgca aggcatatat
     5701 ggatatgttg gaaattttgt tccgccatca agacttcgtc gccatcaaca aaccgggcgg
     5761 catatccgtc caccaagaca gcggcgagac cggactggcc cgaacactcg ccatacagtt
     5821 gggcgtagag cgtgtgtggc tgctgcaccg attggacaaa cagaccagcg gcattttact
     5881 gtttgccctc aacagagaaa gcgcgtccgc ccttagcgga cagtttgccg gcaaaagcat
     5941 caaaaaaacc tatttggcac tatccgaccg caaaccgtcc aaaaagcagg gttggattaa
     6001 aggcggaatg gaaaaatcca gacgcggaat gtggaagttg acgcgcaata cagaaaatat
     6061 cgccgttacc cgattccaca gcatcagtat cgccgaaaaa ctgcggctgt tcatcctgga
     6121 accgcatacg ggcaaaacgc accaattgag agtggcgatg aaaagtttgg gcagtccgat
     6181 tctgggtgac agcttatacg gcggaacaga atccgaaacc atgtttctgt atgcatggaa
     6241 aatacaattt gcctaccaaa accgacaaat cgaaattgtt gcacccttaa aaaacgaatg
     6301 gcagactgaa aatatctctc atgcgctgga agaattttat atggcaaaag caggttaata
     6361 aatataaatt aatttataaa caatatgata aatattttta taaaaatttt tcaagaaaac
     6421 ggttgactgc aaatctcttc a
//
LOCUS       DAWFBO010000110         6406 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFBO010000110 DAWFBO010000000
VERSION     DAWFBO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.110"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WGO87_001534"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WGO87_001534"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0392879.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1297..2652)
                     /locus_tag="WGO87_001535"
     CDS             complement(1297..2652)
                     /locus_tag="WGO87_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0392880.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(2667..2939)
                     /locus_tag="WGO87_001536"
     CDS             complement(2667..2939)
                     /locus_tag="WGO87_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0392881.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3065..3802)
                     /locus_tag="WGO87_001537"
     CDS             complement(3065..3802)
                     /locus_tag="WGO87_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0392882.1"
                     /translation="MKKLSRIVFSIVLLGFSASLSAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAYAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGSKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WGO87_001538"
     CDS             complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WGO87_001538"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0392883.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5127..5720
                     /locus_tag="WGO87_001539"
     CDS             5127..5720
                     /locus_tag="WGO87_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392884.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5832..>6406
                     /locus_tag="WGO87_001540"
     CDS             5832..>6406
                     /locus_tag="WGO87_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ0392885.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIATAAMKA
                     AIPDYMKFGEGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVAKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVDRPANPDAVSTCFKADKSAEAVNNDTEHNTA
                     YFSHFPSKQKSTDGGRCPFLYRFPYFLAAGRA"
BASE COUNT         1752 a         1613 c         1468 g         1573 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctaaaa actgctgctt
       61 cggcttgaag atacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgtttgg taaggaatga atttatgcgc
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcggat tttgaaggaa
      241 atgcacggag gcaaactgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcgg aatacgaagc ggtcgaacaa tactcgcttt ggccggcagt cggaaaccaa
      481 tggcagcttg aggctttgct gatccgccat tggaaaaaac ccgtcaaagt ctggttgaaa
      541 atggattcgg ggatgcaccg taccggtttt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaaac agtcggaata tgtggacagt attgtcaagt tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat gacggaaata cagatggaag cattcgattt gggtacgaaa
      721 gggctggaag gcgaagaaag ccttgccaat tcggcggcta ttttgaatgt tcccgaagca
      781 cgcagggatt gggggcgcgc gggcttggcg ttatacggca tttccccgtt cggaggcagc
      841 gatgacaggc tgaaacccgt gatgaggctt tcaacccgta tttttggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgcgtc
      961 ggtctgattg cctgcggtta tgcggacggt tatccgcgcc gcgccccaag caattccccc
     1021 gtcgccgtcg acggtaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg acgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca atactgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taatcaagtc caaacaaaaa tgccgtctga
     1261 agcctttcag acggcatttc cccatcaaaa ccgcaatcag tttttcatcg attggaccgg
     1321 agcgggaatt ctgccgcccc gtttgacgaa cacttcgcac gaaccttctt ttaccggcat
     1381 cacaggtgcg tagcccaaca ggccgccaaa ctcgacgctg tcgccgacgg ttttaccggt
     1441 taccggaata atgcgcacgg cagtggtttt gctgttgatc atgccgatgg cggcttcgtc
     1501 ggcgatgatg ccggaaatgg tatgagcagg cgtatcgccg ggaacggcaa tcatatccaa
     1561 accaacggag cagacagcgg tcatcgcttc aagtttgtcc aacgtcagca caccggcttc
     1621 ggcagcggca atcatgccct cgtcttctga aacggggata aacgcaccgc tcaaaccgcc
     1681 gaccgcgctg gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     1741 cgccgtcgtg ccgtgtgtac cgcagacgct caaacccatt tcttcaagaa tgcgcgccac
     1801 cgagtcgccg acggcagggg tcggtgccaa cgacaagtcg agaataccaa acgggatatt
     1861 cagcattttt gaggcttcgc ggccgatgag ttcacccacg cgggtaattt tgaaagcagt
     1921 tttcttcact acttccgcaa cttcggtcaa tgtcgttgca tctgaatttt ccaacgcggc
     1981 tttgaccacg cccggccccg atacgccgac attaatcaca gcatccgctt cgcctgagcc
     2041 gtgaaacgcg cccgccataa acggattgtc ttccaccgca ttgcagaaca cgacgatttt
     2101 cgcgcagccg aaaccttcgg gcgtaatttc agccgtgcgt ttgatggttt cgcctgccag
     2161 cttgaccgca tccatattga taccggcgcg cgtactgccg acattgatgg agctgcacac
     2221 gatgtcggtg gtcttcatcg cttcgggaat ggaacggatt aacacctcgt ctgaaggcga
     2281 catgcctttt tgcaccaacg cggaaaagcc gccgataaag gacacgccga tggctttggc
     2341 agccttatcc aaagtttgcg ccacgctgac ataggaatcg gcatgggtag ccgccgcgat
     2401 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcaga
     2461 cagatatttt gccgtcgcca ccaagtcttt gccgaccgtg gtaattttgt tgtaaatgtt
     2521 ttggttcaac acattgatat cgctgctgat gcagtcgtgc agatcaatgc cgatggtaat
     2581 ggtgcggaca tcaaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     2641 ttggatactc atcacattcc tccgactcaa atgcggtgca tcgcttggaa gatttcttca
     2701 ttttgcatac ggatatcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaacc
     2761 tcctgccgag atttggggca tttcgaggta tccaccaaga taatcatggt aaaaaaatca
     2821 tccatcagct gttggctgat attgaggatg ttgattcggt tttccgccaa aattttggaa
     2881 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     2941 ggcttactcc ttgcagatat ccgttaaagt ccgaaattat accaccgttg gattttgaag
     3001 aaatattgtc aacaatatat acatacaaaa tgccgtctga aactatttca gacagcatca
     3061 agattcaggg ttcgattaaa taaccatcct tatcccactg ggttttcctg accaacttgt
     3121 catcctgata aacagcttca ctctttttag aaccatcttc ataccactct aagactactc
     3181 cattacgttt atgatgatgg atagacagtt ccgagagtaa tcgaccgctt tcatcccatg
     3241 tcagaatctt ggccggctca tcattgacca taaccatttc cgttttgata ttgccatcag
     3301 cataccattg tttccatacg ccgtttgcct tattttgttt aaactggatt tcgctttcct
     3361 tgctgccatt acggtaatag cggtaccccg taccttcact caaaccattt ttataaggca
     3421 taaccgcaga ttttttaccg ttcggatacc agttgaccca ctcaccgtcc ggcttaccct
     3481 tgctgaagcc ccccgccatt tttttctgac cattaaaatg ccacaaaatc aacataccgt
     3541 tttgcagggt aggcacaaaa gatttgattt gcgttgaagc aacgatataa ggttcggaat
     3601 atttcttcat cgacggataa taaaaatcct gcgcgtacgc aataccctcc gccacactat
     3661 attgcctgat ataagcggca gaagacatcg tcgccgtcag tttcccgttc tgattaaaat
     3721 aaacagaata ggtctgtgcc gataaagaag ccgaaaaacc caacaggaca attgaaaata
     3781 caatccgaga taattttttc attgcaatag cgatataaaa acaaggctgt gttttagtaa
     3841 tctgttgatt tcaattattt gcaagggaaa agacaattat tttccggtta ggaataaacc
     3901 tattctactg aatatattga agccaagtac gcctatcaac actatattaa aacactgcca
     3961 aaaacaatta acttataaac aatatggtaa ggatttctct gccaagcatc aaacccgaga
     4021 caacgtatcg taaaaatgcc gtctgaaaac aaatcgtctt cagacggcat ttccccttca
     4081 actcactctt cacccaataa ctgctcgcgc gtcaagagga aaacaaaacc gtcgcccccg
     4141 ctggtttcca accaagtaaa aggcaactcc ggatacgctg cttccaatac atccctgtta
     4201 tgcccgattt ccaccagcaa tacacctttg ggattcagaa actttgccgc attcagaaga
     4261 atctgcctgg tggcatccaa cccgtccgcc ccgctgccca atgccaattc cggttcgtgc
     4321 aaatactctt caggcaataa ctcaaccgat tccgcatcca cataaggcgg attggaaaca
     4381 atcaaatcat aagtgccttc caatccttca aacaaatccg tatgaataag ccggatgcgt
     4441 tcttccaaac cataatcttc gacattaatc cctgccactt ccaaagcatc caagctcaca
     4501 tcaaccgcat caatttgggc atcaggataa tgatgcgcca tctgaatggc aaggcaaccg
     4561 cttccggtgc aaagatccaa agcattatgc accaactcat cgtattctat ccaaggacga
     4621 agtccgtcac ccaacaattc ataaataaaa gaacgaggta tgattacgcg ctcatccaca
     4681 tagaaatcaa actctccctg ccatgcctgg tgtgtcaaat aagcggctgg aatgtgttcg
     4741 acagcacgac gctcaataac cgtcagcact tcctcttttt cagcttccaa gagttttgca
     4801 tcaagatatg gggcaagcat atccaaaggc aaattcaaag tatgcagaat caaataagct
     4861 gcttcatcat gcgcattatc cgttccatga ccaaaaaaga gccctgcctc attaaaacgg
     4921 ctgactgcaa aacgtaaaac atcgcggata gtcgtcaatt cttgtgctgc ctgattaaac
     4981 ataatatgaa ccattctgcg tatagatact tttaattata acagaaacaa caagcaaacc
     5041 ttttcatatc gccaaataac cacccaatct acccatacaa ctacataaat gcccgcgcga
     5101 aaaccatcgc ccgaacggaa acgacaatgg ccgacggtat gggcaacctg attggctggg
     5161 aaaaaacggg gcttgttgcc ggtaagcagt ggataaccgc aaaagacgac aaggtgtccg
     5221 atgtctgcaa tgccaacggc gagatgggcg taatcgggct ttacgagcct ttctcacacg
     5281 gcgcattgac gatacccggt catccgaact gccgatgcga ggttgtttcc gtatcgggtg
     5341 gcgaattggg ggaatttgcc gaaaaaaagg agcttcgtaa agcggctatg cagtatgcgc
     5401 gggataactt tatcggcaaa agctatgtca atgaaaacag cgggcatgaa ctgaaggtaa
     5461 cttggcaagg tgtgaaacac gctgcgtcaa aggcaaatca ggcggaatta tccatcatga
     5521 caaaacttga tgacttattg cgctacgcaa aatatgaggg ttcttattcg gataggaaag
     5581 gtcatcctaa tattattgca gcacataagt atcgtgccgt tgccaaggtt gggaatgagt
     5641 ctttaaatat cggtgtgatt gtaagggaat ttccagacga ccataaacat tacgaccatt
     5701 tcatcttgaa ggatgaataa agcccttttg cagtgtcgtt ctggagcgga tagcgttaag
     5761 gcaagtacac ttccagcctt gaaaaagggc tttaaattca gcatgccatt tatacaggca
     5821 ggagtaaacc catgacaaag ttatacgcag aaatcgccaa gatggagacg caggacgacg
     5881 acacggtcaa ggtttggggt tacgcttcaa gcgaggaaat cgattcggac ggcgaagtca
     5941 tcgcgacggc agctatgaag gcggcgattc ccgattatat gaagtttggc gaggggcgcg
     6001 agatgcacgg ctcaaacgct gcgggaacgg caattgaaat caacgttgaa gacgacggca
     6061 taaccttttt cggtgctcat atcatcgacc ccgttgtcgt ggcgaaagtc aaaacaggcg
     6121 tttacaaagg cttttccatc ggcggcagcg ttaccgcccg cgatgatttg aacaagtccc
     6181 aaatcacggg cttgaagctg acggaaatca gccttgtcga ccgccctgcc aatcccgatg
     6241 cggtgtctac ctgctttaag gcggacaaaa gtgcggaagc ggtaaacaac gatacagaac
     6301 ataatactgc atattttagc catttccctt ccaaacaaaa aagcaccgac ggcggccgat
     6361 gccccttcct ttacaggttc ccctattttt tagctgcggg cagagc
//
LOCUS       DAWFBO010000111         6347 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFBO010000111 DAWFBO010000000
VERSION     DAWFBO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.111"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(61..1515)
                     /locus_tag="WGO87_001541"
     CDS             complement(61..1515)
                     /locus_tag="WGO87_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0392886.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(1512..2213)
                     /locus_tag="WGO87_001542"
     CDS             complement(1512..2213)
                     /locus_tag="WGO87_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0392887.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(2210..2989)
                     /locus_tag="WGO87_001543"
     CDS             complement(2210..2989)
                     /locus_tag="WGO87_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0392888.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            3273..4679
                     /locus_tag="WGO87_001544"
     CDS             3273..4679
                     /locus_tag="WGO87_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0392889.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLVGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            4791..6317
                     /locus_tag="WGO87_001545"
     CDS             4791..6317
                     /locus_tag="WGO87_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0392890.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPKYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
BASE COUNT         1370 a         1831 c         1599 g         1547 t
ORIGIN      
        1 atacaaatgc cgtctgaagc cctgtttcgg gtttcagacg gcatttttgc gaacaacttt
       61 ttatgcctgt tctttttggc gcattttttc tgccatcagt tcgtgcaggg ttttcttcgc
      121 cggtttcatc ggcacgcggt tttgcgtcca acccaactgt ttgcgcgggg tcaggttgcg
      181 gaacttggtg gctgcccaac cgaaggcgcg gtaggttttg ctgccgctga aaataccgtt
      241 gaatgtgcgc cacgccattt gttcgccgaa ggtatgcgat gcgccttgcc ctcggatggg
      301 gtgcggcacg gtttcggtcg gcgaacgttg cgcttcaacg cgcaaacgct gcatttgttc
      361 ggtaatcggg atgcgtaccg gacaaacttc cacgcacgcg ccgcacatcg tgcaggcggt
      421 tggcaagtcg cgggtggcat ccagacccaa caggtgaggg gaaataatct cgccaatcgg
      481 gccgggatag gttgtgccgt atgccgcgcc gccgatacgg gtataaaccg ggcaatggtt
      541 catacacgcg ccgcaacgga tacattgcaa ggtgcggcgc atttggtctt cggcataggc
      601 ttggctgcga ccgttgtcaa gcaggaccaa gtgcatttct tgaggaccgt ccagctcttc
      661 gctacggcgc gggccggtaa tcatattgaa ataagtggta atgttctgac caatggcaga
      721 acgcggcagc aggctgtaca agggtgggat gtcggacaat ttcgccacca ctttttcaat
      781 gccggtaata gcgatatgca cgggcggtac ggtggtactc aagcgaccgt tgccttcgtt
      841 ttccaccaga cacagcgtac ccgtttcggc aacagcaaag tttacgccac tcaaaccgac
      901 atcggcagtg ctgtaaatat cgcgcagggc tttgcgggcg aagccggtca gttggtccac
      961 gtcgtccgta agcggagttc caaggttttg gtggaacagt tcactcacct gttctttggt
     1021 tttgtgaatg gcgggcatca cgatatgggt cggtttttcg cctgccattt ggacgatgaa
     1081 ctcgcccaag tcgctttcta ccgctttaat gccttttgct tcaagataat ggttcagctc
     1141 gatttcctcg ctgaccatcg atttgccttt gaccatcagc ttgccgtttt tggctgtgat
     1201 gatgtcgtgg ataatttggc aggcttcggt cggggtttct gcccagtgca ctttcacgcc
     1261 caacttagtc aggttttctt ccagctgctc cagcagggct ggcaatttag acaatgagcg
     1321 ctgacggacg tgttcgcaca aatcgcgcag gctttgcagc tcttcttcgt cggtcaaaac
     1381 ggctttgcgt ttggtcatca gcatatccat cgcggtacgc aggcttttgc gcaaaggctt
     1441 gtcttgaagg gaaattgcgg cgttttgctt gaaagtttcc ggcttcatgt gaaatttgat
     1501 ggtttgcgta gtcatgcgtt ttcctccaaa tcggcagggg aaatgtggtc gggcaggatg
     1561 gcgaggatga ccaaatcgcg cgggccgtgc gcgccgtaag caagcgtcag ttggatgtct
     1621 gcggttttgg acgggccgga aatcaggaat acattggtcg gcataccgtt ttccaccagt
     1681 ttttcgcctt cgacggcatt atgaaactcg ttgtacatct tggacgtatc gaacaggcag
     1741 aaatgcacgg gcggaacgag actcaaagta cgcggttctt cggggctgga aaacagcatc
     1801 agcgtgccgg tgcgggcgat gccgcattgc gcgccgctga agcccgcatc gatgttcgtg
     1861 aaaaactcgg ttttccaagt atcgatttcg cgctcgaagg caatcggttc gatattgctg
     1921 tccgccaatg cggcacgggc aatttgtccg tgttcggtcg ccaagggcag caggatgttt
     1981 ttcaaaccct tgccttctgc cgcttcgcgg aaaacctgca tccaattgct tttcgtcacc
     2041 caataaattt cggttttgac cgcacgcata gcggcagccc aatgtttcag acgctcaact
     2101 tcgctgcccc aagaaacacc catttcacgg taataatcaa aaaccgcagg ttcttccatc
     2161 ggcaatgcgt cggctttttt cagttttgcc aaaatatttt cgcgcgcgct catgctttgc
     2221 ctccggtacg ttccaacaag aaggatgcga tatgtttcgg acgcggcata ttcggttcgt
     2281 ccttagcaat tttgccgccg atgttcatca tacagccgca gtccgcgctg atgatttcgg
     2341 ttgcgccggt ttctttcagc gcggcgactt tgtcggttac catcgcgccg gaaatatcgg
     2401 cttgtttgac ggagaatgtg ccgccgaagc cgcaacattc gctttcgtgg tcgtggacga
     2461 tgcgttcgac gttttccata ccgtcaatca gttgccagcc cgagagatgg acattcattt
     2521 cgcggcgggc ggcgcaggaa gtgtgaacgg cgactttgag cggttcgccc ttgtcttcgg
     2581 gtttgaaacc gatggcaagc aggaaatggg taaactcgat gatgcggccg gcgcaatcca
     2641 cagccctttc ctcgtactcg ctgcctttaa acagcgtcgg ccaatggtgc ttcatcatac
     2701 cgccgcacga accggacggt acgacaatcg gccactcctc ggggaaaagg tcgagttgcg
     2761 ctctggcgac atcaaaggct tcggtcggat ggccggatga ataggcgggc tgaccgcagc
     2821 agctttgcgc catcgggaaa tggacgcgta tgccctgttg ctcgattagg gtaatggcat
     2881 ccatgcctgc ttcgggcata aaaagatcaa ggacgcaagt gccgaaaaaa tagacatcgg
     2941 tcggattgct gtcgtatcgg atgatttttg ggggaatggt tgcgctcatg ttttttgata
     3001 acgggaaacc cgtttttttc tgtagaaagg taagcgttta ctttaagtaa ttgactgttg
     3061 cgggtcaagt ctaattttaa aaaataatcc ggtttttctt acaaactacc ccataacgct
     3121 tactgtacct taatctgata gttttcgata ataattatca ttacaatgca atgccggttc
     3181 gtttgcttgt gaacattcaa gatgccgact ctgacggcat tcagacagca tctgaaaaca
     3241 ataacggcac aaggatgggg tacaatcgct ctatggaaaa aaacacccta tccgcccgcg
     3301 caccatcccc ttggctgccg cttctgctgg caattgccat ttttatgcag atgttggatg
     3361 cgaccatttt aaataccgca ctgcctgaaa ttgccgccga cctgaatgag tcgccattga
     3421 acatgcagct ggcggttatt tcctacacgc tgacggttgc cctgctgatt cctttgagcg
     3481 gttatttggc ggacaggttc ggaacgaaaa aagtcttttt cggttcgatt gccgttttta
     3541 tgctcggatc ggcattgtgc gccgcatcgg gttcgctgtt tgaattgacg ctttcccgtg
     3601 tcgttcaggg catcggcggt tcgatgctgg ttccgatacc gcgtctgacc atcttgcgtg
     3661 tgtacgacaa gtccaagctg ctcaacgcca tcaattatgc ggttatgccc gcattaatcg
     3721 gaccggtttt agggcctttg gcgggcggtt atctggtcga atacgcttcg tggcactgga
     3781 ttttcctgct caacctgccc atcggtcttc tgggtttcat attgggacgc aacatcatgc
     3841 ccgatattaa aggcagcaat atctctttag acttcaaagg ctatctgatt ttttctgccg
     3901 ccgcgtgcct cttgttactt tcggcagaaa gcctgtcgca cgcgctgcct ccgtattttg
     3961 cactgttgcc gctgtgcggc ggactgctgt ttgcacgccg ttatttccga catatgaaaa
     4021 ccgcgtccaa accgatttat tccgccgacc tgtttctgat acgcactttc cgtctaggac
     4081 tggtgggcaa tctgttcagc cgtctcggca tcagctcgat tccttttctg atgcccctga
     4141 tgtttcaagt cgctttcggc ttcggcgcaa gcctgtcggg ttggctggtc gcccccgtcg
     4201 ccctgtcttc gctgctggtc aaaccgctga ttgcaccgct catgaaacgt ttcggctacc
     4261 gcacggtact gctttggaac accaagctgc ttgccgcctt catcatgctg ctcgccctac
     4321 ctgatggaaa ctcgccgctg tggatttgga ttttcctctc gctggcgatc ggcgcgtgca
     4381 actccctaca gttttctgcc atgaacacac tgaccctcgc cgatttgcgc ccgcaacaga
     4441 ccggcagcgg caacagcctg atggcggtca accaacagct tgccatcagc atgggcattg
     4501 ttgtcggcgc attaatcctt aaaaactgga catttctgat accggcttct tcaggtctgc
     4561 attccgcctt ccgtatgacc ctgctcagta tcggcggcat tacccttgcc tcgtcgttgg
     4621 ttttcaagcg tctgcacgtt tcagacggtg caaacctgac acggaacaca ccgtcctaaa
     4681 gcggtccgca cgcaaaactt ttacccgttt caacgtttgg attatgattc cgcacttcca
     4741 tgcgcaccaa ccccaaaaca caggcaatgc cgtctgaaac catatccctg atgaaaacac
     4801 gcagcctaat ttccctttta tgcctccttc tctgttcatg ttcttcatgg ttgcccccac
     4861 tggaagaacg gacggaaagc cgtcatttca atacttccaa acccgtccgc ctggacaaca
     4921 tcctgcaaat ccggcacacc cctcatacca acgggctatc cgatatctat ctgttgaacg
     4981 acccccacga agcctttgcc gcccgcgccg cccttatcga atctgccgaa cacagcctcg
     5041 atttgcaata ctacatctgg cgcaacgaca tttccggccg actgctgttc aacctcgtgt
     5101 accttgccgc agaacgcggt gtgcgcgtac gcctgctgtt ggacgacaac aacacgcgcg
     5161 gattggacga cctcctgctc gccctcgaca gccatcccaa tatcgaagtg cgcctgttca
     5221 accccttcgt cttacgaaaa tggcgcgcac tcggctacct gaccgacttc ccccgcctca
     5281 accgccgcat gcacaacaaa tcctttaccg ccgacaaccg cgccaccata ctcggcggac
     5341 gcaatatcgg cgacgaatac ttcaaagtcg gtgaggacac cgttttcgcc gacctggaca
     5401 tcctcgccac cggcagcgtc gtcggcgaag tatcgcacga cttcgaccgc tactgggcaa
     5461 gccattccgc ccacaacgcc acgcgcatca tccgcagcgg caacatcggc aagggtcttc
     5521 aagcactcgg atacaacgac gaaacgtcca gacacgcgct cctgcgctac cgcgaaaccg
     5581 tcgaacagtc gcccctctac caaaaaatac agacaggacg catcgactgg cagagcgtcc
     5641 aaacccgcct catcagcgac gaccctgcaa aaggactcga ccgcgaccgc cgcaaaccgc
     5701 cgattgccgg gcggctgcaa gacgcgctca aacagcccga aaaaagcgtc tatctggttt
     5761 caccctattt cgtccccaca aaatccggca cagacgcact ggcaaaactg gtgcaggacg
     5821 gcatagacgt taccgtcctg accaactcgc tacaggcgac cgacgttgcc gccgtccatt
     5881 ccggctatgt caaataccga aaaccgctgc tcaaagccgg catcaaactc tacgagctgc
     5941 aacccaacca tgccgtccct gccacaaaag acaaaggcct gaccggcagc tccgtaacca
     6001 gcctgcatgc caaaaccttc attgtggacg gcaaacgcat cttcatcggc tcattcaacc
     6061 tcgacccccg ttccgcacgg ctcaatactg aaatgggcgt tgttatcgaa agccccaaaa
     6121 tcgcagaaca gatggagcgc acccttgccg ataccacacc caaatacgcc taccgcgtta
     6181 ccctcgacag gcacaaccgc ctgcaatggc acgatcccgc cacccgaaaa acctacccga
     6241 acgaacccga agccaaactt tggaaacgca tcgccgcaaa aatcctatcc ctgctgccca
     6301 tagaaggttt attatagaaa tgtagcggat taacaaaaac cagtacg
//
LOCUS       DAWFBO010000112         6293 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFBO010000112 DAWFBO010000000
VERSION     DAWFBO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.112"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(549..1133)
                     /gene="rnhB"
                     /locus_tag="WGO87_001546"
     CDS             complement(549..1133)
                     /gene="rnhB"
                     /locus_tag="WGO87_001546"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0392891.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDTLAEMIKDQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLVVHPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1201..3096)
                     /gene="mnmG"
                     /locus_tag="WGO87_001547"
     CDS             complement(1201..3096)
                     /gene="mnmG"
                     /locus_tag="WGO87_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0392892.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTYEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3421..4848)
                     /locus_tag="WGO87_001548"
     CDS             complement(3421..4848)
                     /locus_tag="WGO87_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0392893.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5008..5997
                     /gene="pdxA"
                     /locus_tag="WGO87_001549"
     CDS             5008..5997
                     /gene="pdxA"
                     /locus_tag="WGO87_001549"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0392894.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAAEAVEAGRLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFCIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRRERINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
BASE COUNT         1322 a         1638 c         1829 g         1504 t
ORIGIN      
        1 gtcattcccg caaaaacaaa aaaatcaaaa acacaaaact gaaatatcgt cattcccgcg
       61 caggcgggaa tctaggtctg tcggtacgga aacttatcgg gaaaaacggt ttttccaacc
      121 ctgagactcc ggattcctgt tttcgcagga atccggtttt ttgagtttca gtcattttga
      181 taaattcttg cagctttggg tttctagatt cccgcttttg cgggaatggc ggtttggaag
      241 ttacctgaaa ttcaaaaaaa cggaaaccgg acggactgga ttcccgtttt cacgggaatg
      301 acggatttta ggtttctttt ttgattttct gtttttcgcg ggaatggcgg tttggaagtt
      361 acctgaaatt caaaaaaaaa aaaacggaaa ccggacggat tggattcccg cctgcgcggg
      421 aatgacggat tttaggtttt tttttgattt tctatttttc gcgggaatga cggtttgggt
      481 tctttctctt tggagttgcg atgccggaaa tgccgtctga aggcttcaga cggcattttt
      541 gtgccggttt aaaacaaggc ctgctgcgcg agcaggtttc tgacgggggc gaagtcgcgg
      601 cggtgttcgg gcagcacgcc gtattgtttg agggcttcca gatgctgctt cgtgccataa
      661 cctttgtgtt tgtcgaaacc gtattgggga tggcgttgcg ccagtgcgta catttctgca
      721 tcgcgtgcgg tctttgccaa aacggatgcg gcggagattt cgatgatttt gctgtcgcct
      781 ttgacgacgg cttcggcagg gatacctaaa tgttctggaa tgcggttgcc gtcgatgaat
      841 attttttcgg ggtgcacaac caaaccgtca acggcgcgtt tcatggcaag catggtggcg
      901 tgcaggatgt tgaggctggc gatttcttcg ggcgtggcgg cggcgacgtg ccacgcggcc
      961 gcctgatctt taatcatttc ggcaagcgtg tcgcgttttt tctcgctgag ttttttggag
     1021 tcggtcagtc cgggtaggtc aaacgtttcc ggaaggatga cggcggcggc aaacacgctg
     1081 ccgactaaag gtccgcgtcc ggcctcgtcc acgccggcgg tcagtatgtg catgatgttt
     1141 cctgtcggga tggtacgatg ccgtctgaaa agggtttcag acggcatcgt accgatgtgt
     1201 ttatttcgcg tctttaaacc cgcgcttcaa atgcaccatc agcagtgcga cggcggcagg
     1261 cgttacgccg gaaatgcggc ttgcctgccc gacggtttcg ggtttgtgct ggttgagctt
     1321 ttgctgcact tctgccgaca agcctttgac tttgccgtaa tcgatgccgt cgggcagttt
     1381 taaggtttcg atgtcgcggc ggctgtcgat ttcttcgttt tggcggtcga tatagccttg
     1441 gtatttgact tggatttcga cttgttcgat gacttcggcg gagaggtttt cagacggcat
     1501 cgcgccttcg agcgtcatca gcgcggcgta gtcgaggttc gggcggcgta acaaatcgtg
     1561 caggttggct tctcggctga gtttttgtcc gaatacgcgg atttgttcgt cttcggcgag
     1621 tttttgcggt gtgtaccacg ttgttttcaa acgttggatt tcgcgttcga cggcttcgcg
     1681 tttttcgttg aacatacgcc attgcgcttc acccaccaag ccgattttgt agccgtcttc
     1741 ggtcaggcgc atgtcggcgt tgtcttccct gagttgcagg cggtattcgg cgcggctggt
     1801 gaacattcgg tagggttcgt tcacgccttt ggtgatgagg tcgtccacca atacgccgag
     1861 gtaggcttgt tcgcggcgca gcaggagcgg gtcttgtccg cgcacatatt gcacggcgtt
     1921 cgcgcctgcc aataaacctt gcgcggcggc ttcttcgtag ccggtcgtac cgttgatttg
     1981 cccagcgaaa aacagtccct caatggtttt ggtttcgagg ctggctttga ggttgcgcgg
     2041 gtcgaagtag tcgtattcga tggcgtagcc ggggcgcagg atatgggcgt tttccaaacc
     2101 tttcatactg cggacgagcg cgatttggat gtcgaacggc aggctggtgg agataccgtt
     2161 aggatagtat tcgtacgtag tcaggccttc gggttctagg aaaatctggt ggctgtcttt
     2221 atcggcgaaa cggttgattt tgtcttcgat agacggacaa taacgcgggc ccacgccttc
     2281 gattttgccg gtaaacatcg ggctgcggtc gaagcctgag cggatgatgt cgtgggtttg
     2341 cgtgttggta tgcgtaatcc agcaggacac ttggcgcggg tgcatttcgg cgttgccgcg
     2401 cacggacatg acgggaacgg gtgtgtcgcc gggctgttcg gtcagttggg aaaagtcaat
     2461 cgtgcgtccg tcgatgcgcg gcggcgtgcc ggttttcaga cggccttgcg gcagcttcaa
     2521 ttcgcgcaaa cgtccgccca gcgatttggc ggcggggtcg ccggcgcgtc cgccctcgta
     2581 gttttccaaa ccgatgtgga ttttgccgga caaaaacgtg cctgcggtca acaccacggc
     2641 gcgtgcttta aactccacgc ccatcgcggt gattacgcct gaaatgcggt cgccatcgag
     2701 cgttacgtct tcgacggctt gttggaaaag gtcgaggttt tcttggtttt ccaacatttc
     2761 gcggatggcg gctttatata ggatacgatc cgcctgcgcg cgcgtggccc gtactgccgc
     2821 gcctttgctg gcgttcagac ggcggaactg gatgccggat ttgtcggttg ccaacgccat
     2881 cgcgccgccg agcgcgtcga gttcgcgcac caaatgccct ttgccgatgc cgccgatgga
     2941 agggttgcac gacatttgtc cgagcgtttc aatattgtgt gtgagcaaaa gcgtctgcgc
     3001 gcccatacgg gcggcggcga gcgcggcttc tgtgccggcg tgtccgccgc cgacgacgat
     3061 aacgtcgtag gttttggggt aaatcatgtg ggtcatgtgt gtattgcctg tcggtgtttc
     3121 agacggcatt tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa
     3181 gagaacgatt ctctaaggtg ctgaagtacc aagtgaatcg gtttcgtact gcttgtactg
     3241 tctgcggctt cgtcgccttg tcctgatttt tgttaaacca ctatattcaa tatgccgtct
     3301 gaaaaacgaa atggattcaa aagtaaaggg ttgggattgt acgcttgttc gccctgtttt
     3361 tacagtgtgc ggaaagggaa aagccgcttc gcggggaagc ggctccggta agggcgggat
     3421 ttaccaaacg tcggatttga tacggcgttt caggcccgga tgttcggaaa gtttgaactc
     3481 ggggtctttg cccattttca gcttggcggt gtaatcgcgc agcagcataa acgccaaggg
     3541 cgagagcagc aggatggcga caaggttgat ccacgccata atgcccatcg ccatatccgc
     3601 catatcccag accaaaggca cattggcaac cgcgccgaaa tagacccacg ccaaaaccag
     3661 catacggaaa acggcggtaa tcagccaatg gcttttgatg aattggacgt tggactcggc
     3721 ataggcatag ttgccgataa cggtggaaaa ggcaaacata aacaggatga cggcgaggaa
     3781 gcccgcgccc cattgcccca cttggctgac aatcgccgcc tgcgtcaacg ccgcaccgct
     3841 caaatcgccg taaggctgtt ggtaaatcaa gatgatgaag gcggtgcaag aacaaacgat
     3901 gatggtatcg acaaacacgc ccagcatttg aatcatacct tgcgaaacag ggtgtttcac
     3961 ttcggcggcg gcggcggcgt tcggcgcgga acccataccc gcctcgttgg aatacaggcc
     4021 gcgtttgatg cccatcatca tcgtttgcga aatcagaccg ccgagtaagc cgcctgctgc
     4081 cgcgtcgaat ttgaacgcgc ccgaaaaaat ctgaccgaac acgtccggaa tcatcggaat
     4141 attggtcaaa atgatgaaaa gcgcgataaa gaggtacaaa accgccatca gggggacgac
     4201 gatttccgcc gctttagata tgcgcctgat gccgccgaag ataatcggcg cggttaaaat
     4261 caccagggcg acgccgacat aatgaggctc ccaaccccac gccgctttga cggtatcggc
     4321 aatggtattg gtctgaaccg cttcaaacac aaagccgaaa cagaaaatca ggctcagggc
     4381 gaacaacacg cccagccatt tctgccccag cccttgagtg atgtagtagg cagggccgcc
     4441 ccggaaatgg tggttgtcgt agtcgcggac tttaaagagc tgcgccagcg aagattcgac
     4501 aaacgccgaa ctcataccga ttaaggcggt tacccacatc caaaacaccg cgcccggtcc
     4561 gccgactttg atggcgatgg ccacgcccgc gatattgccc acgcccacgc ggctggcaag
     4621 gccggttaca aatgcctgaa acggcgtgat gccgtgaggg tcgtccccct gtttgcggcc
     4681 gccgagcatt tctttgatgc tgcgcccgaa caggcggaat tggacaaagc ccgtggttac
     4741 ggtgaagaaa agccccgtac ccaaaagcat ataaaccaag tatgaccaca tcggatcgtt
     4801 gatggcgccg acccagccgt gcagccattc ggtaaagttc tcgttcatat cgcttcctta
     4861 aagttgaaac tcgcacatat tggcggtatg caagcagggt ttaaattttg taaacgccca
     4921 ttctagcaga ttgtcaacaa aatcagaaaa atttacatcg ccgcgcggct gcggcgttag
     4981 aatcgcattt tgtttggagc aaacacgatg aaacagcctg tttttgccgt tacttccggc
     5041 gagcctgccg gcatcggccc cgatatttgt ttggacttgg cgtttgcacg cctgccctgc
     5101 cgctgcgcgg tattgggcga caaaaaccta ttgcgcgcgc gcgccgaagc cttgggcaaa
     5161 agcgtcgtct tgcgcgactt cgatccggaa tcaggcggcg cggcatacgg cgagctggaa
     5221 gtgctgcaca tccctgccgc cgaagcggtt gaggcagggc ggctcaatcc cgccaacgcc
     5281 gcctatgtgc tgcaactttt ggacaccgcg ctcgcaggca tttcagacgg cattttcgac
     5341 ggcatcgtta ccgcgccgct gcacaaaggc atcatcaacg acgcgcgcgc aagcacaggt
     5401 tttttcagcg gacacaccga atatctggcg gaaaaaagcg gcacggggca ggtcgtgatg
     5461 atgcttgccg gcaaaggcct gcgcgtcgcc ctcgtaacga cccacctgcc gctgaaagac
     5521 gttgccgccg ccatcacgca accgctgatt gaaagcgtcg cacgcatttt gcatcacgac
     5581 ttaaaacaca aattctgcat caaaaatccc aaaatcctgg tcgccggact caacccccac
     5641 gccggtgagg gcggacacct cggacacgaa gaaaccgaca ccattatccc cgcgctcgaa
     5701 aacctgcgcc gcgaaaggat aaacctcgcc ggcccgtatc cggcggacac attgttccaa
     5761 ccgtttatgc tcgaaggtgc ggatgccgta ttggcgatgt accacgacca agggctgccc
     5821 gtgttgaaat accacagttt cgggcagggc gtgaacatca cactggggct gccctttatc
     5881 cgcacctccg tcgatcacgg caccgcgctc gatttggcgg caaccggcag ggcggattcc
     5941 ggcagcctga taactgccgt ggagaccgcc gtcgagatgg cgcgcggcag cctttaaaga
     6001 tgataaaaga cccgtcattt ccgcgcaggc gggaatccgg tctgttcggt ttcagttgtt
     6061 tttgggtttc gggtaatttc caaatcgtca ttcccgcgca ggcgggaatc cagaccttcg
     6121 ggcgacggca atattcaaag attatctgaa agtttgaggt tctagattcc cgttttcacg
     6181 ggaatgacga aaggttgcgg gaatccggct tgttcggttt cggttttttt tgaggtttcg
     6241 ggcaacttct aaaccgtcat tcccgcgcag gcgggaatcc aggcctttgg gcg
//
LOCUS       DAWFBO010000113         6270 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFBO010000113 DAWFBO010000000
VERSION     DAWFBO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.113"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(64..624)
                     /locus_tag="WGO87_001550"
     CDS             complement(64..624)
                     /locus_tag="WGO87_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0392895.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPA
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1041..2081)
                     /gene="mutY"
                     /locus_tag="WGO87_001551"
     CDS             complement(1041..2081)
                     /gene="mutY"
                     /locus_tag="WGO87_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0392896.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2244..2378
                     /locus_tag="WGO87_001552"
     CDS             2244..2378
                     /locus_tag="WGO87_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine acetyltransferase"
                     /protein_id="HEZ0392897.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     "
     gene            2365..3189
                     /locus_tag="WGO87_001553"
     CDS             2365..3189
                     /locus_tag="WGO87_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEZ0392898.1"
                     /translation="MWVFEKAKKTEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVII
                     SCVSKCCTCDNCKIQLYSHCCNGGWILGYMIDGTQAEYVRTPYADNSLVPLPDNVNEE
                     VALLLSDALPTAQLYSPAAIIVCDMDENRLKLAKELGATHTINPASGDVSKQVAAIVG
                     EDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKLEKLWIKKLAITTGL
                     VNANTTEMLMKAISSSSVDYTKMLTHRFKFSELEKAYDVFKHAAENQAMKVVLEAD"
     gene            complement(3213..3431)
                     /locus_tag="WGO87_001554"
     CDS             complement(3213..3431)
                     /locus_tag="WGO87_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392899.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3446..4084)
                     /locus_tag="WGO87_001555"
     CDS             complement(3446..4084)
                     /locus_tag="WGO87_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0392900.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     RNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4265..6100)
                     /gene="edd"
                     /locus_tag="WGO87_001556"
     CDS             complement(4265..6100)
                     /gene="edd"
                     /locus_tag="WGO87_001556"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0392901.1"
                     /translation="MNPTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NNTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDPIRFDSVTGELNVLINETE
                     WNAREVEQIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1387 a         1498 c         1771 g         1614 t
ORIGIN      
        1 gctaaatatc tagcaaaaaa gtgcggtcaa aattcaccgc acttttcgtt tcgaacaatc
       61 gaattattta atcacgccac atgccatacg tgggccgcca ccgccaagtg gagctggatg
      121 atcggagtga ttatcaccac ccgtgtggat cataatagag tgaccgcgaa catcatctaa
      181 atgtttaaga cgtggtgcta aaacaggatt tgttgctgtg ccatcatgca atacagttaa
      241 tgcaggtaaa tcacctaagt gtgcatcatc ttgccatggg taaccatgtt gttttgcacc
      301 tttaggatcc cagtgaccgc ctgcgcctaa accagctgtc aatttacctt ctttttcttt
      361 tggctcacag cttgggtttt catggatgtg gaaaccatgt aagccttcgc ttaatccttg
      421 taaatcaggg gtaaacacaa gaccatagtt agattcagta atagtcactg tacccacatc
      481 tttgttaccg tttgctggat caagttgttg cactttcact tcaatagaag cacctttagg
      541 tatcgtatta tgctcgtgtg cttgcgcaac accaactgaa catactgcac taaccgctag
      601 tgctaataag gttttcatat tcattgcaat ctcctttatt gcagtaaaaa ctcgcgacta
      661 ttttagtcaa ttttgtttta aaagccgata aatttttacg aaaattttac attaattgtt
      721 atttgcagaa aattagaacc cgttatcacg tcttggataa atgccaaaac cattgtgatt
      781 cagagaatta aaaagaaaaa tttcggaatc ctcatccttt tctgaaacct gattgcggta
      841 tccatggctt tgtctttgtc atttaagttt gtgttcatgt ttttgacctt ctttgctttt
      901 taaatccttt ttaaattgcc gacttcttcc gttccaaatg tcccatttga acattatccc
      961 accgcctaaa atacgaccca aattcatcgg ttgttcttgc tgcgatacct gcaaacaaca
     1021 gttcaatggg tttattttgt ttattctaac ctattaccgt ttaaataaat ttccaaaggc
     1081 ttgggcaggc cgtaatcttt caaatgcgcc ggctttatcc aaatgccgtc tgaagggctt
     1141 tcagacggca tttgtccttc aaacggcgta atcatcagca gccggtgcgt cagacggtgg
     1201 gtcagggcgg tttgttcgtc catatctgcc atggtcaggg agaatttggc ggcaaagtcg
     1261 gaaagcccgt tcaaactttc aaaacacggc acgcaataca gcccgcccca aatgcctttg
     1321 gcggggcgtt tttccagcaa aatcgcgccg tcccggttgc ggacaatcag ccagtaaagc
     1381 ggcagggtcg gtacttcggc ggcggttttt ttgcgcggca gctcggcggt gcggttttgc
     1441 ttttttgctt cgcagatgtc cgccatcggg cattggtggc acaagggttt cgtccgtttg
     1501 cacacggtcg cgcccaaatc catcaaacct tgtgtatagg caggcatatc ggcgttttca
     1561 gacggcagca ggctttcggc aagtgtccag agcgagtttt caaatttttt gtcctgcgga
     1621 ttgccgtcgc gggcgaacac gcggcagagt acgcgtttga cgttgccgtc caaaatggtt
     1681 tcgcggcggt tgaaggagaa ggcagaaatg gcggcggcgg tgcttctgcc tacgccgcag
     1741 agggtttcca agtctttgcg ctccgacgga aacgtgccgc cgaattgcct gacgacttgt
     1801 tgcgcggctt tgtgcaggtt gcgcgcgcgg ctgtaatagc ccaagcccgc ccacaacgac
     1861 aacacttcgt cttgcggcgc ggcggcaagc gtctgaacgg tcgggaattt ttctaagaag
     1921 cgcggatagt agtccaacac ggtggcgact tgcgtttgct ggagcatgat ttcggaaagc
     1981 cagacgcaat aagggttttt gacctgccaa gggaggtggt ggcgaccgtg ttgtttttgc
     2041 cagcggatga gccgttcgga gaaggggatg ggtgtgttca ttaatatcaa tcggtggttt
     2101 tatttatatt taaaacggta tgttattgta taacattgtg aaaataattc ttattgactt
     2161 attttttgta ggggtatata actcatataa agaaacttta ttgcggtatt gaaattattt
     2221 atcaacaagc aaggagtatc agcatgaaag caatggttta tcacggcgca aacgacatcc
     2281 gttttgaaga aaaaccccgt ccgcagatta tcgatccgac cgatgcggtg gtgaaaatcg
     2341 tcaaaaccac gatttgcggt accgatttgg gtatttgaaa aggcaaaaaa aaccgaagtc
     2401 gccgacggtc gtattctcgg tcatgaaggc atcggtattg tagaagaagt cggcgaggct
     2461 gtaaaaaaca tcaaagtcgg cgataaagtc attatttcat gcgtcagcaa atgctgtact
     2521 tgcgacaact gcaaaatcca actttattcg cattgctgca acggcggctg gattttgggc
     2581 tacatgattg acggcacgca ggcagaatac gtccgcacgc cttatgccga caacagcctc
     2641 gtgccgctgc ccgacaacgt caacgaagaa gtcgccctgt tgttgagcga cgccttgccg
     2701 accgcccaac tgtacagccc cgccgccatc attgtttgcg atatggacga aaaccgtttg
     2761 aaactggcga aagagttggg cgcaacccat accattaacc ctgcttccgg cgatgtaagc
     2821 aagcaagttg ccgctatcgt cggcgaagac ggcgtggact gcgcgatcga agccgtcggt
     2881 atccctgcaa cttggaatat gtgccaagac attgtgaaac ccggcggcca tatcgccgtt
     2941 gtcggcgtac acggtcaatc cgttgatttc aaactggaaa aactgtggat taaaaagctt
     3001 gccatcacta ccggcctggt aaacgccaat accaccgaaa tgctgatgaa ggcaatttcc
     3061 agcagctccg tcgattacac caaaatgttg acccaccgtt tcaaattcag cgaattggaa
     3121 aaagcctacg acgtgttcaa acacgccgcc gaaaatcaag cgatgaaagt ggttttggaa
     3181 gcggattaat cggccttgaa tcagttgttt ttttaagcgg atgccgtctg aatgcgtttc
     3241 agacggcatt ttttcagccg cgtagtatgg gctgtccggc tgctgtcgaa tgaactgaac
     3301 gttattttta ttatgcggaa ggcggatggg cattttaggg gcagtatgtc ggagtggtgc
     3361 tgccgtgtat gccgactgcg ccgccaagcg gcaaaatgcc gtctgagact ggaaagggct
     3421 tcagacggca tttttacgat gcgaatcagg ctttgggttt caatgccgcc gcttctttgg
     3481 cgagacgggt gatggtgtcc cagtctttgt ttttcacggc ttctttcggc gtcagccatg
     3541 agccgccgac gcacaggacg ttgggcagtg ccaagtaatc gggcgcggtg gcgaggctga
     3601 tgccgcctgt cgggcagaag cgaacatcgg cgtaagggcc gtacagggct ttgagcatgg
     3661 ctttgccgcc aacgacttcg gcagggaaga gtttgagggt gtcgatgccg tgttccaaag
     3721 ccagttggat ttcgcccggg gtggcaacac cgggaatcag gggaatgccg ctgttgcggc
     3781 cggctttggc gagggattcg tgcagacccg ggctgatggc gaaaaccgcg cctgcgtctt
     3841 cgacggcttt gagctgttcg ggattggtta ccgtacctgc gccgacgatg gcgttgggca
     3901 cctctttggc aatcaggcgg atggcatcga ggccgacagg ggtgcgcagg gtgatttcga
     3961 gggtagggat gccgccttcg acaagggcgt gggacaaatc gatggcggtg cttaagtcgt
     4021 caatcgccat taccggcaca actgcgccgg cggtcaaaat ttcgcggggg gtcagtttgg
     4081 acatttcggt tctccgggta ggatagggta ttttattaag atgggacagg ttgtaggtcg
     4141 gattcaagaa accgacatct tggacggctg tgtcggattc gagaatccaa cctacgtcta
     4201 tcttaaaacg cttgaataat ttttcagacg gccttcggtc tgaaaaggcc gtctgaaacg
     4261 cgcatcaggc aaattcgccg ccgaagctca tcgcaccggt ttccgcgctg ctggtcatgc
     4321 tgcggaagcc ggcgaagagt tcgcgaccgc agccttgttg gttcgcgccc aagtcgattt
     4381 gttcgacttc gcgggcattc cattcggttt cgttgatcag aacgttgagt tcgccggtaa
     4441 cggagtcgaa gcggatcggg tcgccggtac gaattttggc gatgttgccg cccatcaggg
     4501 cttcgggggt catgtggatg gaggccggaa ctttgccgga cgcgccggac atacggccgt
     4561 cggtcagcag cgccactttg aagccgcggt cttgcaggat gcccaaaggc ggggtcagtt
     4621 tgtgcaactc gggcataccg ttggcacgcg ggccttggta gcggacgacg cagacgaaat
     4681 cgcgttccaa ttcgccgcgc tcaaacgcag ccaacacttc gcgttggtcg ttgaacacga
     4741 tggcaggcgc ttcgataatg cggcagcctt cgcgcacggc ggacacttta atcacgccgc
     4801 gaccgatgtt gcctttcatc aggcgcagac cgccgtctgg ggagaacggg ttgtcggctt
     4861 tgcgtaggat gtcgtcgttg ccgctggttt cgggggcttc tcgccattcg agtttgccgt
     4921 cgatgaggaa aggctctttg gtgtagtggc gcataccgtg tccgacgacg gtatcgacat
     4981 cgtcgtgcaa caggcctgcg tccagcaatt cgcggatgac gaaaggcagt ccgcccgctg
     5041 cggtaaagtg gtttacgtcg gccttgccgt tcggataaac gcggatgagc agcgggatga
     5101 tggaggaaat ttcatcgaag tcgtcccagt tgagaatcac gccggccgca cgcgccatag
     5161 cgacgaggtg catggtgtgg ttggtcgagc cgccggtcgc catcagaccg atcagggcgt
     5221 tgataaagga tttttcggtc aacatttcgc ccaaaggttt gatggtgttg tttttaatgc
     5281 cgcgcgcgag gtgtccggcg gcgtagcggg tcagcgcttc acgcaggtcg gtgtaagggt
     5341 ggacgaaagc ggcggcaggg aggtgcacgc ccatcatttc catcatcatt tggttggagt
     5401 ttgccgtgcc gtagaaagtg caggtgcccg ggctgtggta agaacccatt tcgcttttca
     5461 aaagttcgtt gcgtccgacc ttgccttcgg cgaaaagctg gcgggtgcgg gctttttctt
     5521 tgttgccgat accgctggac atcgggcctg cggggacgaa gatacctgga atatgaccga
     5581 acgaaagcgc gcctatcatc aagcctggaa cgattttgtc gcatacgccc ataaacaggc
     5641 tgccgtcaaa catttgatgc gacagcccga cggcggtact catggcaatc acgtcgcggg
     5701 agaacagcga cagttccata ccggcgtagc cttgcgtgat gccgtcgcac atggcgggtg
     5761 tgccgccggc gacttgggcg gtcgcgccgt ttttctgcgc ttcgtctttg atttggtcag
     5821 ggaagtcttt aaacggctgg tgtgcggaaa ccatgtcgtt gtaggcggtg atgatgccta
     5881 agttggggac ggtttcctga agcatttcga ttttgatgct tttgggcatg gcggcatagc
     5941 cgtgcgccaa attgctgcag ccgagttggt tgcgctccaa gcgtcccatt tgtttggcgc
     6001 tgcggatttt cgccagatat ttttcacgcg tcggacggct gcgttcaata atgcgcccgg
     6061 tgatttcggc gagtttggag tggataggag tggggttcat gttcggtctc ctgtcggaag
     6121 tgggtggtat ggggattttg tttgcgcgtt tctgattttt tgtattgggt gtggggaatt
     6181 tggcgtttca gacgaccttt caaatcttac aaggggtcgt ctgaaaacct cactatagtg
     6241 gattaacttt aaaccagtac ggcgttgcct
//
LOCUS       DAWFBO010000114         6263 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFBO010000114 DAWFBO010000000
VERSION     DAWFBO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.114"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..131)
                     /locus_tag="WGO87_001557"
     CDS             complement(<1..131)
                     /locus_tag="WGO87_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0392902.1"
                     /translation="MLKTLTFAALHFTTAFSVTYVLTGSIGVSGAVALVDPLINTVV"
     gene            254..1207
                     /locus_tag="WGO87_001558"
     CDS             254..1207
                     /locus_tag="WGO87_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0392903.1"
                     /translation="MNHKKIVVLDADTLPGRAFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIQDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPAVREGYVAFENAVRTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLKARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1281..3338)
                     /gene="metG"
                     /locus_tag="WGO87_001559"
     CDS             complement(1281..3338)
                     /gene="metG"
                     /locus_tag="WGO87_001559"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0392904.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGKLKDVSDSALLAKLTAQSEAIAECYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTATN
                     AARFLNLDAITWTNTRVTLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESKYEKAAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATAEGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(3454..5292)
                     /gene="glmS"
                     /locus_tag="WGO87_001560"
     CDS             complement(3454..5292)
                     /gene="glmS"
                     /locus_tag="WGO87_001560"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0392905.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKSGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATKGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5574..6092
                     /locus_tag="WGO87_001561"
     CDS             5574..6092
                     /locus_tag="WGO87_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392906.1"
                     /translation="MKQMLLAVGVVEVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1336 a         1469 c         1911 g         1547 t
ORIGIN      
        1 aaaacgacgg tgttgattag ggggtcgacc agcgcgaccg cgccagatac gcctatactg
       61 cccgtcagca cataggttac actgaaggcg gtggtgaaat gcagtgcggc aaaagtcagg
      121 gttttaagca tcatcctctc ccgaattgaa aatttacgga gagatgataa agattatcat
      181 caggccgtgc cggtttaaat ttgctattta ctgttagtgt aaataaatca attttaaagt
      241 aaggatagaa atgatgaatc acaaaaagat agtcgttctg gatgcggata ctttgcccgg
      301 cagggctttt cacttcgatt ttccgcacga gcttgcggtt tacggtacga caggtgcgga
      361 tgaaacggcg gaacgggtgc gcgatgcaca tattgtcatt accaataagg taatgatttc
      421 cgccgacatt attgcggcta atccgcagtt ggagctgatt gccgtcagtg cgaccggtgt
      481 gaacaatgtc gatattgggg cggcggaggc ggcgggcgtt gcggtatgca atgtccgcgc
      541 ctacggaaac gaatcggttg cggaacatgc ctttatgctg atgattgccc tgatgcggaa
      601 tttgcctgcc tatcagcgtg atgttgcggc aggattatgg gaaaaatcgc cgtttttctg
      661 ccattacggc gcgccgattc aggatttgaa cggcaaaacg ctggcggttt tcggacgcgg
      721 caatatcgga cggacgctgg caggatacgc gcaggcgttc gggatggggg tggtgtttgc
      781 cgaacacaaa cacgcgcccg ctgtgcgcga aggctatgtt gcctttgaaa atgcggtacg
      841 gactgccgat gtgttgtcgc tgcactgtcc gctgaacgca caaactgaaa atatgatagg
      901 cgaaaatgag ttgcggcaga tgaagcccgg tgcggttttg attaattgcg ggcgcggcgg
      961 gctggtggat gaaaacgcgc tgcttgccgc actcaaatac gggcagatcg gcggggcggg
     1021 cgtggatgtt ttgacgaatg agccgcctaa aaacggcaat cctctgttga aggcacgact
     1081 tcccaatctg attgttacgc cgcataccgc gtgggcaagc cgcgaggctt tggacaggct
     1141 gtttgatata ttgttggcga acattcacgc ctttgtgaaa ggcgaggcgc aaaaccgggt
     1201 ggtttgagct tgtcgggatg acgaaaaaat gccgtctgaa cgcctcaagg gttcagacgg
     1261 cattgttttg acatttctgc ttaaccgact ttgtcgcccg gttgtgcgcc tgcatccaca
     1321 tccaagagct tcagtttgcc ctctgccgtg gcggcactca aaatcatacc ttcggatacg
     1381 ccgaattttg ccattttgcg cggggcgaag ttggcgacgg cgatgaccat tcttccgttt
     1441 aactcggcag ggttgggata agacgcggcg ataccggaga agatgatgcg tttttcaaaa
     1501 ccgaaatcga ggtcgaattt caaaagtttg gtgctgccct cgacggcttc gcagttcaag
     1561 actttggcga cgcgcatatc aattttcata aagtcgtcga agctggcctg ttcggcggct
     1621 ttttcgtatt tgctctcttc ggcggcaggt gcggctgcgg cggcgatgct ttgtttgttg
     1681 gcttcgatta aatcgtccac ttgtttttgc tccactcgtt gcattaaatg tttgtatttg
     1741 ttgatggcgt gtttgcccaa ggttaccctt gtgttcgtcc aagtaatcgc gtccagattg
     1801 aggaaacgcg cggcattggt ggcggtttgc ggcaatacgg gggcgaggta ggcggtcagc
     1861 atcgtaaagg cgttgatgag ttcgctgcat acttcgtgca ggcgttcgtc ttggccttct
     1921 tgtttggcga gttcccaagg tttgttggca tcaacgtatt cattaacaat gtctgccaat
     1981 gccatgatgt cgcgcagggc gcgggcgtat tcgcggtttt cgtagcattc ggcaatggct
     2041 tcgctttgcg cagtcagttt tgccagcaat gcgctgtcgg aaacgtcttt gagttttcct
     2101 tcaaagcgtt tggcgatgaa acctgacgcg cgggcggcga tgttgacgta tttgccgacg
     2161 aggtcgctgt tgacgcgcga gataaagtct tgcaggttca aatcgatgtc ttcgattttg
     2221 ctgttgagtt tggcggcgat gtagtagcgc atccactcgg ggtttaggcc ttgttccaga
     2281 taggatttgg cggtaataaa cgtgccgcgc gatttggaca ttttttgtcc gtccacggtc
     2341 aaaaagccgt gtgcgtacac gccggtcggg gcgcggtggc cggagaaatg cagcatggcg
     2401 ggccagaaca gggcgtggaa atagaggatg tctttgccga tgaagtggta catctcggtt
     2461 tggctgccgg ctttgaagta ttcgtcgaaa tcgatgccga tgcggtcgca caggttttta
     2521 aacgatgcca tgtagccgac gggcgcgtcc agccagacgt agaagtattt gcccggcgcg
     2581 tcggggattt caaaaccgaa atacggcgcg tcgcgggaaa tgtcccagtc ggacagggtg
     2641 gtttcttcgc cttcgcccag ccattctttc attttgttga gggcttctgg ttgcagatgg
     2701 ggtttgccgt cgtgcgggtt gttgccggaa gtccatgctt tgaggaagtc ggcgcattcg
     2761 cccagtttga agaagaagtg ttcggattcg cgcaattcgg gtttcgcgcc ggaaacggcg
     2821 gaatacgggt taatcagttc ggtcggggaa taggtcgtgc cgcagacttc gcagttgtcg
     2881 ccgtattggt cttgggcgtg gcatttcggg cattcgcctt taacgaagcg gtcgggcagg
     2941 aacatttgtt tttcggggtc gaaaagctgc tcgatgacgc ggctctcaat cttgccgttg
     3001 gctttcagcg cgcggtaaat atcttgggaa aactgtttgt tttcagggga atgggtgctg
     3061 taataattgt cgtagccgat gttgaaaccg gtaaagtcgg cgaggtgttc ttcgcgcact
     3121 ttggcaatca tgtcttcggg cgcgatgcct tgtttttgcg cggcaagcat cacgggcgtg
     3181 ccgtgggtgt cgtcggcgca gcagtagtag cattcgttgc cgcgcagttt ttggaagcgc
     3241 acccaaacgt cggtttggat gtgttcgacc atgtggccga ggtggatgct gccgttggca
     3301 tagggcaggg cggaggtaac taagattttg cgtgtcatat tgtgctttgc aaacaatggg
     3361 taaaggcgga ttataccgca aatcaaacgg ggaaatgccg tctgaagcct gaaaaatcgg
     3421 gcttcagacg gcatttttgc aaaacggcgg gagttattcg acggttacgg atttcgccag
     3481 gttgcgcggc ttgtccacat cggtaccgcg tgcgagggcg gtgtggtagg cgaggagctg
     3541 cacggggata gtatgcacga cgggggacag tttgccgacg tggcgcggtg cgcggataac
     3601 gtgcacacct ttggtggcat taaaattact gtcgaggtcg gcaaagacga aaagttcgcc
     3661 gccgcgcgcg ccgacttcct gcatattggc tttgactttg tccaacaggc tgtcgttggg
     3721 tgcgatgacg acgacgggca tgttttcgtc caccaaggca agcggcccgt gcttcagttc
     3781 gccggcagga taggcttcgg cgtggatgta ggtgatttcc ttcagtttta acgcgccttc
     3841 gagggcaatc gggtaatgga tgccgcgccc caaaaacagc gcgctggttt tcttggcaaa
     3901 ctgttgcgcc caggcggcaa tttgcggttc gaggtttaaa gcgtgctgca cgctgccggg
     3961 aagctggcgg agttcttcgg tgtaacgtgc ttcgtcttct tcggaaacca aaccgcgcac
     4021 tttcgccagc gttaccgcca aaccgaacag cgcaaccagc tgcgtggtaa atgctttggt
     4081 tgaggcgacg ccgatttccg caccggcacg ggtataaagc acgaggctgc tttcgcgcgg
     4141 cagggcggat tccatcacgt tgcagatgga gaggctgtgg cggtgtccca aggattttgc
     4201 gtatttcaac gcctccatcg tgtccagcgt ttcgccggat tgggaaatgg taatgaccag
     4261 ttggtccgga tcggcaatca cgctgcggta tcggtattcg ctggcgattt cgacatcggc
     4321 cgggattttg gcgatggatt ccaaccaata tttggcggtc agcgcggcgt aataggacgt
     4381 gccgcaggca aggattttga cgctgcggat gctttcaaac acgcttttgg cgtttttgcc
     4441 gaagttttcg gggatgaagc cgccgtcgag gaaaacctcc gccgtgtctg caatcgcgcg
     4501 cggctgctca tggatttcct tttgcataaa gtggctgtac ggtcccagtt ccaaagaggc
     4561 gagcgagagt tcggatacct tgactttgcg ttcggcaggc aggccgcttt tatcggtcag
     4621 ccgtttgatg ccgtctgaag ccagcagcgc gatgtcgccg tcttcgaggt acgccacgcg
     4681 gcgcgtaaag gcgatgacgg cggatacgtc cgaagcgata aaggtttcat cgtcgcccaa
     4741 agcgaccaaa agcgggcagc ccatacgcgc cacaactaat tcatcaggct tgtcttgggc
     4801 aataaccgcg atggcgtatg cgccgtggaa acgtttgacc gcttcttgta ccgcttcaaa
     4861 cagcctgccg ccgttttgcg cgtattcgtg attgatgctg tgtgcgatga cttcggtatc
     4921 cgtttgcgat tcaaaacggt atcccaaacc ttccaagcgt ttgcgttcgc tttcaaagtt
     4981 ttcgatgatg ccgttgtgga cgaccgcaat catcccgccg ctgatgtgcg ggtgggcgtt
     5041 cggctcggta acgccgccgt gtgtcgccca gcgcgtatgc ccgatgccga tgccgccgct
     5101 gatgcctttt tcgcgtgccg cgtcctccat aagctgcacg cgtccgacgc ggcgcacgcg
     5161 tttgattttg ccgtcggtgt tgacggcaat gcctgatgag tcataacccc ggtattcgag
     5221 gcgtttgaga ccgtcggtca gaaaatcgac gacgttgtga tgggcgcgga tggcgccgac
     5281 gataccgcac ataactgttc cttagtatcc ggttgaaaaa aaacaggcgc ggacggctcc
     5341 cgtgccgcac cttccgcttc ggattataaa ccgcctcccg cgccgaaaaa cagcaaaatg
     5401 ccgtctgaag gcttgggctt gctcaaaaaa gggagggatt tccccgcctg accgggatgg
     5461 gcgttcagac ggcattgcct gctgccggtt tatagttttt acaaatcaat attgacaagc
     5521 tgaaaaaaaa aaacaatata ctcgctcggt cttaatgtta acggagtatg gaaatgaaac
     5581 aaatgctttt ggccgtcggc gtggtggagg tgttggcggg ctgcggcaag gatgccggcg
     5641 gttacgaggg ttattggcgc gaaaaatcgg acaaaaaaga gggtatgatt gccgtcaaaa
     5701 aagaaaaagg caattacttc cttaataaaa tcaacgtgtt tacaggtaag gaagaatcta
     5761 tgcttttgtc tgaaaaagac ggcgcgcttt cgataaacac ggggataggg gaaatcccga
     5821 tcaaactttc cgatgacggg aaagagctgt atgtcgaacg caggcagtat gtcaaaaccg
     5881 atgcggcgat gaaggacaaa atcatcgccc atcagaaaaa gtgcggacaa acggcacagg
     5941 catatctcga cgcgcgaaat gcgttgccgt caaaccagac gtatcagcag catcaggcgg
     6001 cgatcgagca gttgaaacgg cggtttgaag ccgagtttga cgaattggaa aaagaaatca
     6061 aatgcaacgg caaaccgaca ttgttgtttt agcgtaggac aaccgggagg atgccgccgt
     6121 ccgaatcgga cgtgcggttt ctgtaccggt acgggcgggc aggaatgtcc gctttttttg
     6181 ttcggacgcg tttgaatacc cgtttgattc cgtccgtttg caaagggtat ttttgttcgg
     6241 gcggaaatta tagtggatta aca
//
LOCUS       DAWFBO010000115         6262 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFBO010000115 DAWFBO010000000
VERSION     DAWFBO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.115"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(391..825)
                     /locus_tag="WGO87_001562"
     CDS             complement(391..825)
                     /locus_tag="WGO87_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0392907.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGO87_001563"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGO87_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0392908.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WGO87_001564"
     CDS             complement(1556..2398)
                     /locus_tag="WGO87_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0392909.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WGO87_001565"
     CDS             2653..3291
                     /locus_tag="WGO87_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0392910.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WGO87_001566"
     CDS             complement(3743..4543)
                     /locus_tag="WGO87_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0392911.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(5522..6163)
                     /locus_tag="WGO87_001567"
     CDS             complement(5522..6163)
                     /locus_tag="WGO87_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0392912.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMMMSEKSGVPLYFDRAL"
BASE COUNT         1672 a         1568 c         1296 g         1726 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacaacccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 catcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttgccgct gccttctccg cgccccgttc cgtctccttt gataccggtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgcg gcaggcgcgc
     2221 ctgcaccttc gggtgcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttta cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctgctg cttatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt ggccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gtttgctgga taacaaagtc
     4741 caaaccaata gcagataaaa taaggcatcc accccctttc ttcattaagg ataagagata
     4801 tcgagaaaca aatcataact aaacagaaaa aattttggag ataaagccat ttcattcccc
     4861 tattcaagaa tctggccaag ataggtattt tgtatactct agaaaagaaa ggcatttcca
     4921 agggaaacat atcagataaa aacttttgtt tattttttac tatagataga accctatttc
     4981 tcaagataaa tccaaatgca atgccaatta tcattattaa taaacaaagg atagtataac
     5041 tatggataac cttcattaaa caaaaagaag aaatggcaga taaattacat acgatatagt
     5101 ggaatataaa atatttacgg tctaaacctt gttcagttgc aattttttta tacttacctt
     5161 gcgcaaaaaa atcaaagaaa tccattaaac tatctttcac attagaaatt taaaagctaa
     5221 ataatacaac aaacaatgtg aagtactatt catggtttat ttaaaaaata atactattct
     5281 gaacattatt tagatacaga aattaacaaa ttagaactaa acaagctttt aaatacttta
     5341 attttattgg aaagctataa aaggaactat aactttacac actagtcact tctttttaag
     5401 aggcaaaagg gattgggaag gtcgtcttgg agataagcac tggtatttcg gccaatggta
     5461 aatagagttt acctcaaata gggtagaacc tccttcatct gtcagttaat aacagccact
     5521 tttacaatgc cctgtcaaaa taaagcggca cgcccgattt ttcactcatc atcatcaaat
     5581 aacccatcac cttttgaggc cattcgatac cgcgcaccac ggtcagattc ctcaaaacgg
     5641 ggaaaaccaa aatatcctcc atgccgattc cgccgttgat gccgtctgaa gcaccgtcca
     5701 tcaaattctc caactcttgc aaatctgcgt ttatgcgttc gagatattgg gcggttttat
     5761 tcaaattggc ggaaaagctg ccgatgcttt tctctttttt gtctgtaaaa tattttaccg
     5821 cttccggcgt tgcaaattcg ggcagcccga ttttgatcac gcgcggctgc accagtttgt
     5881 cgttgtatcc gcccaccttg tccagccacg cccgtatctc ggggcggact tcgtctttca
     5941 gacggtcttc gcggtcgaaa tgccgcacaa tgtccaaact ctcgcccata aacgaaccgt
     6001 cttctttttg caggacgggc acttgtttcg caccgatcat accgatcggc gttgcctcgt
     6061 cgtcgtttgc cagcacggct tcttcaacgt ccgcgccaaa cagcccggca gccatccgcg
     6121 cacgcacaca aaacgggcaa tggtcgtaaa tatacagttt catcaaaata ttcctcgtca
     6181 acctgtcggt accggctacc ttaacacccc gcgccacccg aaacaagttt atcttcccgc
     6241 ctatgcaccg taaataaata ag
//
LOCUS       DAWFBO010000116         6139 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFBO010000116 DAWFBO010000000
VERSION     DAWFBO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.116"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            205..1638
                     /gene="lpdA"
                     /locus_tag="WGO87_001568"
     CDS             205..1638
                     /gene="lpdA"
                     /locus_tag="WGO87_001568"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0392913.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1733..2020
                     /locus_tag="WGO87_001569"
     CDS             1733..2020
                     /locus_tag="WGO87_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392914.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2119..3285
                     /gene="sucC"
                     /locus_tag="WGO87_001570"
     CDS             2119..3285
                     /gene="sucC"
                     /locus_tag="WGO87_001570"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0392915.1"
                     /translation="MNLHEYQAKELLAGYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            3296..4186
                     /gene="sucD"
                     /locus_tag="WGO87_001571"
     CDS             3296..4186
                     /gene="sucD"
                     /locus_tag="WGO87_001571"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0392916.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(4737..>6139)
                     /locus_tag="WGO87_001572"
     CDS             complement(4737..>6139)
                     /locus_tag="WGO87_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ0392917.1"
                     /translation="FYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSIWKVILYL
                     LISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSKEAAEMIF
                     GKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDGIDIRPSQ
                     IPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGLQNQNTKL
                     QDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPWNAPNLHD
                     EYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQREYSIYL
                     LGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQTTSVEIPK
                     FMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTETEYLIFS
                     ELSKALDVGTPFKNKQ"
BASE COUNT         1753 a         1368 c         1407 g         1611 t
ORIGIN      
        1 gatgtagcgt aatgtagtat cgtgctacaa taggctcaac aaacgattga gaccgtctga
       61 aacatttgat tcgaatgaat cggcagatat ggactttcag acggcttttt cttaaaacca
      121 tcaaaacgca gtcattcaaa ataaaaaaga aacgaaaagt atcgttttta ttttgagata
      181 ctgctaaaag caaaggatga cacgatgtct caatatgatg tagtagtgat tggtgcaggc
      241 ccgggcggat acgttgccgc catccgtgcc gcacaactgg gtttcaaaac tgcctgtgtc
      301 gatgcaggcg ttaacaaagc aggcaatgcc cctgcattgg gcggtacttg cttgaacgta
      361 ggttgtatcc cttctaaagc cctgttgcaa tccagcgaac atttccacgc agcgcaacac
      421 gagtttgccg aacacggtat cactgtcggc gacgtaaaat tcgacgcggc caaaatgatt
      481 gagcgcaaag atgccatcgt gaccaaactg accggcggcg tgaaattcct gttccaaaaa
      541 aataaagtaa ccagtctgtt cggtacggct tcctttgccg gtaaaaatgg cgatgcttac
      601 caaatcgaag tcgataacaa aggagagaaa accgttatcg aagccaaaca cgtcatcgta
      661 gcgaccggtt ccgtaccgcg tccgctgcca caagtcgcta tcgacaatgt gaacgtattg
      721 gacaacgaag gcgcattgaa cctgaccgaa gtacctgcca aactcggcgt gatcggttcc
      781 ggtgtgattg gtttggaaat gggttccgta tggaaccgcg tgggtgcgga agttaccatt
      841 cttgaagccg cgccgacctt cctggctgcc gccgaccaac aaatcgccaa agaagccttc
      901 aaatacttca ccaaagagca aggtctgagc atcgaattgg gcgtgaaaat cggcgacatc
      961 aagtctgaag gcaaaggtgt ttccgttgct tacgaaactg ctgccggcga agccaaaacc
     1021 gaagtattcg acaaactgat cgttgccatc ggccgtattc caaacaccaa aggcctgaac
     1081 gcggaagccg taggcttgga aaaagacgag cgcggcttta tcaaagtaga tggcgaatgc
     1141 cgtaccaacc tgcctaacgt atgggcaatc ggcgacgtgg ttcgcggccc gatgttggca
     1201 cacaaagcca gcgacgaagg cgttgccgtt gccgaacgca ttgccggtca aaaaccgcat
     1261 atcgacttca acaacgtacc gttcgtgatt tacaccgatc ctgaaatcgc ttgggtgggt
     1321 aaaaccgaag agcagctcaa agccgaaggc gtggagtaca aaaaaggtac ttcaggtttt
     1381 ggtgcgaatg gtcgcgcatt ggcaatgggc aaagccaaag gtacggttaa agtgttggca
     1441 gacgccaaaa ccgaccgcat cttgggcgta cacatgattg gtccggttgt cagcgaattg
     1501 gttaccgaag gcgtgactgc gctcgaattc ttcgccagca gcgaagacat cgcccgcatt
     1561 atccatgccc acccaacctt gtccgaagtg gttcacgaag ctgcattggc ggccgacaaa
     1621 cgcgctttgc acggttgata gacattaagg ccttcgacaa attgaatgtt ccgagagctc
     1681 cgttttctga tttataattc cgtcagacaa acaaacagca tttacattcg ttatgaacaa
     1741 agaaatagtc ggtattttct ttataccgat gggcatcatc agcatgtgta tggccgcatt
     1801 gtggcagatg tatgtgatga tgaccgaaac ttatacgctc aaccgtttca aagataaaga
     1861 attggtttgg cgcgtggcat tgttgtttat cagtttcagc cttgccgttt atctgctctg
     1921 tccgaattcg cgtaaaaaag gcatcgtctt ttttattctc gggggaggcg gtgcagccat
     1981 gtatctgctg gcgcggatgt ggttgccttt cagcaagtga aacgacgatt ttccgaccgc
     2041 cgaaaggtag tctgaaacgc acgggcttgc catttggagg caaactcggg gcattccact
     2101 aatctaaagg agaaatccat gaatttacac gagtatcagg ctaaagaact gctggctggc
     2161 tacggtttgc ccgtacaagg cggtattttg gcacacaacg gcgaagaagc cgctgcagct
     2221 tacgacaaat tgggcggcaa attcgctgtt gtcaaagcac aagtacacgc cggcggccgc
     2281 ggtaaagcgg gcggcgtaaa agtcgttaaa agccgcgaag aagctaaaga agtggctgaa
     2341 agcctgattg gcaccaactt ggtaacttac caaaccgatg ccaacggcca acctgtcaac
     2401 agcgttttgg tttgcgaaga tatgtatccg gttcaaaccg agctgtactt gggcgcagtg
     2461 gttgaccgtt ctacccgccg cgttacattc atggcttcta ccgaaggcgg cgtggaaatc
     2521 gaaaaagttg ctgccgaaac tccagaaaaa atcttcaaag taaccgttga tccgctggtc
     2581 ggcctgcaac cttgccaagc tcgcgaagtt gccttccaac tgggcttgaa agacaaacaa
     2641 atcaacgagt tcgtcaaact gatgaccggc gcgtacaaag cgtttgtcga aaacgacttc
     2701 gccctgtttg aagtcaaccc gctggcagtt cgcgaaaacg gcgcactggc ttgcgtggac
     2761 ggcaaaatcg gcatcgacag caacgcgctc taccgcctgc caaaaatcgc tgaattgcgc
     2821 gacaaatctc aagaaaacga acgtgaactg aaagcttctg aattcgacct gaactatgtt
     2881 gccctggaag gcaacatcgg ctgtatggtt aacggtgccg gtttggcgat ggccaccatg
     2941 gacatcatca aactcaaagg cggtcaacct gccaacttct tggacgttgg cggcggcgca
     3001 accaaagacc gcgtggttga agcgttcaaa ctgattttgg aagacaaatc cgttaaaggc
     3061 gtattgatca acatcttcgg cggtatcgta cgttgcgaca tgattgcgga agccatcgtg
     3121 gcagccgtta aagaaatcaa cgtcgacgtt cctgtcgttg ttcgtttgga aggtaacaac
     3181 gccgaactcg gcgcgaaaat cctgaacgaa tcaggcctga aactgacttc tgcagacggc
     3241 ctgaatgacg cagccgaaaa aattgttgca gccgtaaacg cctaaggaga aaagaatgag
     3301 cgtattgatt aataaagaca ccaaagtatt ggttcaaggt ttcaccggta aaaacggtac
     3361 tttccactcc gaacaagctc tggcttacgg cactaaagtt gtcggcggcg ttaccccggg
     3421 caaaggcggt caaacccacc tgaacctgcc tgtattcaac accatgaaag aagctgttaa
     3481 agaaaccggc gcggatgcat ccgtaattta cgttcccgct ccgtttgtgt tggattctat
     3541 cgttgaagcc gttgattcag gcgtaggctt ggtcgttgtg attaccgaag gcgtacctac
     3601 tttggacatg ctcaaagcca aacgttactt ggaaaccaac ggcaacggta cccgcttggt
     3661 cggccctaac tgcccgggcg tgattactcc gggcgagtgc aaaatcggca ttatgccggg
     3721 ccacatccac actccgggcc gcatcggtat tatttcccgt tccggtacat tgacttacga
     3781 agccgtggca caaaccacca aactgggctt gggtcaatca acctgtatcg gtatcggcgg
     3841 cgacccgatt cctggtatga accaaatcga cgcactgaaa ctcttccaag aagacccaga
     3901 taccgacgcc atcatcatga ttggtgaaat cggcggtact gcggaagaag aagcagccga
     3961 atacatccaa tccaacgtaa ccaaacctgt tgtaggttac attgctggtg ttaccgctcc
     4021 taaaggcaaa cgtatgggcc acgccggtgc gattatttcc ggcggtaaag gtactgccga
     4081 agaaaaattc gccgctttcg aaaaagccgg tatcgcctat actcgcagcc ctgccgagtt
     4141 gggcacgacg atgctggaag tgttgaaggc aaaaggtttg gcataatcag gtttgacaac
     4201 tgattaaaca tcaaatgccg tctgaaaccg gaaatcgggt ttcagacggc attttgtttg
     4261 tcatttcaaa aagaggcagc ctcaacatac ccacattatt ttttcccttt tggggcagtc
     4321 agagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggggatt
     4381 ttggggaatt ttgcaaaggt ctcgggctaa gtgtgcctgt ttgcgcctaa aaggcggccc
     4441 ggatgcctga ttatcgggta tcctgggagg attaaggggg tattggggta aaattagtgg
     4501 atatttgaaa cgaaaacagc cgaaaacctg tgtttgggtt tcggctgtcg ggagggaaag
     4561 gaattttgca aagctctcgt attggctttg aagttccgtg taattcacag gtagggcgtg
     4621 tggcacagcc acgcacgcgg tcggttgggt atgcaggcta cggctttctc tgttgaaact
     4681 gcaacacgtc agctaccaac cgtgtgcgta gcgcacacgg ttggttggga tacaagttac
     4741 tgtttgttct taaatggagt accaacatca agggcttttg ataattctga aaatattaaa
     4801 tattcagttt cagtttttat tttaggagaa atatttgcat aatttctatc tttaaagcac
     4861 caatggatat atggtttcgt ttcatcttct gggttatcta aataagcaat aacattcaag
     4921 tcaaataaaa attgcaaaaa ctcattagca gtactcataa atttaggtat ttccactgat
     4981 gttgtttgta agtgcttttt caaacgttca aatgctttta aaaaatcact atatttaaat
     5041 ctatctttcc cgtttaaaaa ttcaaaaaat ttcaggaaat tttgataatc actttgacta
     5101 taataaaaca aaagatgatc tttgatttca ccaagtaaat atatcgagta ttctctttga
     5161 aaagaagtat tatcaaaatc ttctgctacg acataatctt ccttactttt cttatttttt
     5221 tgtagcaaag taagcatctg aagaatatcg cgaggtcgat aatacgattt tcttaggaag
     5281 ctaataaatg aagttaaatt tttatactca tcatgtaaat taggagcatt ccatggaaaa
     5341 taataatccc atgagttgcc tttttctaaa ctatcttgtt tttcttgctg ggttctcaaa
     5401 agatgatcaa aaacgccaaa aatctttgaa cttctataag atttataatc cgtcctccag
     5461 tctaaaaata ctgaattatc ttgaagtttg gtattttgat tttgtaaacc taatgaatca
     5521 aagatatcag gtctaatcaa taacacaact ctcatccttc ccttactatc tttaatggaa
     5581 gggaagatat cattatttaa catccatatg gcgttagcaa gaccttttac acactcatga
     5641 tattcatcaa atggaatctg tgatggtcta atatctatcc catcaataaa caaaatatga
     5701 ttatctttta gctttaactg agataaagca tctttaaatt ttctttcaat aaaacctaaa
     5761 tttgcttgga atttactttc tgtaaaagtt atttgttggg attcctcttc acctagttta
     5821 acaaattttc caaaaatcat ttccgcagct tcttttgaat tttctattaa agttattgct
     5881 tgtacaattt ccggatcaaa agcgccataa taatattcat ttatagcctc atctaaggct
     5941 ttaaatttat taaatattga agataatatt ccgttttctt tacatttgat ttgatttgat
     6001 atcaacagat ataaaatgac tttccaaata cttgtaaaat ctgaaacagt taagtgtctt
     6061 gctttcttta gctgaataaa ttttgaataa tcggtttcac gaacaaactt agtagtggca
     6121 tgtatgtttt tatagaagt
//
LOCUS       DAWFBO010000117         6101 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFBO010000117 DAWFBO010000000
VERSION     DAWFBO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.117"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(268..642)
                     /locus_tag="WGO87_001573"
                     /pseudo
     CDS             complement(268..642)
                     /locus_tag="WGO87_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(948..2207)
                     /gene="rho"
                     /locus_tag="WGO87_001574"
     CDS             complement(948..2207)
                     /gene="rho"
                     /locus_tag="WGO87_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0392918.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2427..4811)
                     /gene="ppsA"
                     /locus_tag="WGO87_001575"
     CDS             complement(2427..4811)
                     /gene="ppsA"
                     /locus_tag="WGO87_001575"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0392919.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            5222..6043
                     /locus_tag="WGO87_001576"
     CDS             5222..6043
                     /locus_tag="WGO87_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0392920.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1323 a         1418 c         1733 g         1627 t
ORIGIN      
        1 gggcagtcct tccaacaacg gcgacaggtg tttgcactca tgggcattgg cggaggtata
       61 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa cagtacggaa
      121 cccattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
      181 ccgccgtact ggtttttgtt aatccactat aaaaatttat ggggctgtcc tagataacta
      241 ggataaactc gattttacta attgttttca aatggaaatt tgaactttta tctcactgtt
      301 gttaaaacgt cgttcgtacc cctttaaata cagctcaaaa tgcgctttgg gaatgccgtc
      361 aaacttgcgt aaatgacgtt ttgcccggtt tcaaaagttc ccaattccat tgatatggtt
      421 ttgtcgttca gcaaaataac tttcatctgc ttctactttg ccgtcaaaca tttccaaatg
      481 cggactgttt tgataaataa gtaatcgtaa acgatgaaaa taataggctg cggtattttt
      541 attaacgcct actaactctg ctgctgttct tgcagttaca cctgcgacaa acagttcaat
      601 gagtttattt tgtttatact ggcttagacg actttttctc ataggggcaa ctctaactta
      661 atttgaattt ccctagttat ctaggacagc cccaaattta tacaaaaatg agcgcggttc
      721 ggcgcaacct tgaatcaagt tcccgcatcg gttttcattg ccggtacgga tgcgttcaag
      781 ccggtttttc aaaggccgcg ctttcggcaa gcggacacgg acactgccga cggttgcgcc
      841 gttaacgggg ggagggagga gctgcgccga ccgtgtgaat gaaagtgccg tctgaaaccc
      901 gattttcagg cttcagacgg catttcgcat taatgcgggc ggcgcgttta tttgccgcgc
      961 atcagttcaa agaaatcgtc gttgttttta gaggctttga ttttcccgtt taagaactcg
     1021 gtagcttcga tttcatccat cgggtgcagg aacttgcgta agagccacat acgttgcaac
     1081 tggtcgttcg gcacaagcag ctcttcgcgg cgcgtgccgg atttgttgat gttgatggcg
     1141 gggaagaggc gtttttccgc catacggcga tcaaggtgca attccatatt gccggtgcct
     1201 ttgaattctt cgtaaatcac atcgtccata cggctgccgg tttcaaccaa tgcggtggcg
     1261 ataatggtca gcgaaccgcc ttcttccacg ttgcgcgccg caccgaagaa acgtttgggg
     1321 cggtgcagcg cgttggcatc gacaccgccg gttaggattt tgcccgaggt aggcacgacg
     1381 gtattgtagg cgcgggcaag gcgggtaatc gaatccagca ggatgaccac gtcttttttg
     1441 tgttctacca tacgcttggc tttttcaagc accatttcgg caacttggac gtggcgttgc
     1501 gccggttcgt caaaggtgga ggaaaccact tcgccgcgga cggaacggct catttcggtt
     1561 acttcttcgg gacgctcgtc aatcaagagg acgatgagtt cgacttcggg atagtttgag
     1621 gtaacggcgt gggcaatgtt ttgcagcatc acggttttac cgcttttggg cggggcaacc
     1681 aagagggcgc gctgaccttt gccgataggg gaaatcaggt cgatggcacg tccggtcagg
     1741 ttttcttcgg actttaagtc gcgttccagc ttcaactgtt cggtcggaaa cagcggggtc
     1801 aggttttcaa acaggatttt atggcggcat acttccgggt ggtcgccgtt gatggtatca
     1861 agcctgacca gggcaaaata gcgttcgttg tcttttggga cgcgcacgct tccttcgatg
     1921 gtgtcgcccg tatgcaggtt gaagcggcgg atttgggtgg gcgagacata gatgtcgtcg
     1981 gggccggcaa gataggacgt gtccgcgctg cggaggaagc cgaagccgtc gggcaggatt
     2041 tcaagcgtgc cggagcaggt gaaaccctcg ccttttttca tcatctggcg gacgatggca
     2101 aatacgaggt cttgtttgcg gaatcggttg gcgttttcga tgccgtgttc ttccgccaat
     2161 tctaagagtt tggaaatgtg cagggtttgt aattcggaga cgtgcataat aatgatgtat
     2221 tttgaagagg aaaaagacag gcagatgccg tctgaaagaa gaagctgacc gttgccggtt
     2281 gctcggggaa aggggaattg taggcagtcg gcgcgtgggt gtcaaatatt atcgcggacg
     2341 gggcatcggc aggaaatgcc gtctgagcgg agctgcttgg aaaaaaatac ccccgcgctt
     2401 ttcaggctcg ggggtatggg cattgattat ttgttcaatt cattcgccaa atacagccaa
     2461 gtttcgatga cggtatccgg gttcagggaa acgctttcaa tgccttcctc aaccagccat
     2521 ttggcgaagt ccggatggtc ggacgggcct tgaccgcaga tgccgacata tttgttctgc
     2581 ttgcggcagg cggagatggc aaggtgcagc atcactttga cggcagggtt gcgttcgtca
     2641 aacgattcgg ataccaagcc gctgtcgcgg tcgagaccga gggtcagttg ggtcatgtcg
     2701 ttcgagccga tggagaagcc gtcgaagtat tgcaggaatt gttccgccaa taccgcgttg
     2761 ctcggcagct cgcacatcat aatcaggcgc aggccgtttt tgccgcgttc caagccgttt
     2821 tctttcaggg ctttgacaac ggcttcggct tcgcccaagg tgcggacgaa cggaatcatg
     2881 atttcaacgt tggtcaaccc catttcatcg cggacgcgtt tcaaggcttt gcattccaag
     2941 gcgaaacagt ctttgaagtt gtcggcgaca taacgcgccg cgccccggaa gcccaacatc
     3001 gggttttctt catgcggttc gtatacgttg ccgccgacta ggttggcgta ttcgttggat
     3061 ttgaagtcgg acatacggac gatggtttta cgcggataaa ccgatgcggc caatgtcgcc
     3121 acgccttcgg cgattttatc gacgtagaag tcgacagggg acgcgtaacc ggcgatgcgg
     3181 cgggtaattt ccgcttttaa ttcgtcgtct tgtttgtcaa attccaacaa ggctttcggg
     3241 tggataccga tttggcggtt gatgataaat tccatacgcg ccaagccgat gccttcgctg
     3301 ggcaggttgg cgaagctgaa tgcgagttcg gggttgccga cgttcatcat gactttgacg
     3361 ggtgctttgg gcatattgtc caaggcgaca tcggtaatct gtacgtccaa caggccggca
     3421 tagatgaagc cggtatcgcc ttcggcacag gatacggtaa cttcttgacc gtttttcagc
     3481 aattcggttg cattgccgca gccgacaacg gcaggaatgc ccagttcgcg cgcgatgatg
     3541 gcggcgtggc aggtacggcc gccgcggttg gtcacgatgg cggaagcacg tttcatcacc
     3601 ggttcccaat ctgggtcggt catgtcggta acgagtacgt cgccggcttc gacggaatcc
     3661 atctcggaag catctttaat caggcgcacc ttgccctgac cgactttctg accgatggcg
     3721 cggccttcgc acaagacggt tttttcgccg ttgatagaaa agcggcgcag gttgcggctg
     3781 ccttcttctt gggatttcac ggtttcggga cgggcttgca ggatgtagag tttgccgtcc
     3841 aagccgtcgc gtccccattc gatatccatc gggcggccgt agtgtttttc gatggtcagt
     3901 gcgtaatgcg ccaactcagt aatttcttcg tcggtaatgg agaagcggtt gcggtcttcc
     3961 tcggggacat cgacgttggt tacggattta ccggcttctg ctttgtcggt aaaaatcatt
     4021 ttgatgtgtt ttgaacccat ggttttacgc aggatggcgg gcttgcccgc tttgagcgtg
     4081 ggtttgaaca cataaaattc gtccgggttg accgcacctt gtacgacgtt ttcgcccaga
     4141 ccgtaagagg aggtaacaaa gacgacttga tcgtagccgg attcggtgtc gagggtgaac
     4201 atcacacctg atgcgccgct gtcggaacgc accatgcgtt gaacgccggc ggaaagggcg
     4261 acgatgtcgt gttcgaagcc tttgtggacg cggtaggaaa tggcgcggtc gttgtacagg
     4321 gaggcgaata catggtgcat cgcttcttta acgttttcca atccgttgat gttcaagaag
     4381 gtttcctgtt ggccggcaaa tgatgcgtcg ggcaggtctt cggcggttgc ggaagagcgt
     4441 acggcaacgg aaatgtccgc accgccggca tcggcaacca ttttgttcca tgccgcttcg
     4501 atttcggcat cgagctgttc ggggaagggc gtgtccaaaa tccattggcg gatttctttg
     4561 ccgacgcgtg ccagttcggc aacgtcttcg acatctaatt ttgccagtgc ggcggaaatg
     4621 cgttcgctca gaccgttgtg tgcgaggaat gcgcggtagg catcggctgt ggtggcaaag
     4681 ccgccgggga cgcgaacgcc tttttcggtc agctgactga tcatttcgcc cagcgaggcg
     4741 tttttaccgc ccacgcgttc aacatctgtc atacgcaggt tttcaaacca gattacgtag
     4801 ttgtcggcca tttgtgtgtc caatccaaaa tatgttaaaa aagaaacaaa tccgcgtgct
     4861 tattttaagc gattcgttcc tctgctgtca tgtgttttat ccgttttaaa atcatgatgc
     4921 cgtctgaaaa attgcggttt cggcgtgtgt agcggtttga aacttgcagc cggtatactt
     4981 ctttttttgg gtattttctt tgtaaaacag gtggtttgaa taggttaatg ttttttctgt
     5041 ttgatttttt tgtttatttt ttaaaatttt ctgccaaaaa atactttata taaataattt
     5101 tatttcaaaa ttatattgtg tctgtttggg tgtaatccga ggtaggcgtg ctgcgggtgc
     5161 tttccttgtg tctgccgctg ctgttatgat gatattttta ttctgtattt aaggatggaa
     5221 gatgagcagt ccgcgccatg tgttttacat ttccgaccgt accggtctga ctgccgagaa
     5281 tatcggcgag gctttgctga accagtttgg caatctgtcg ttcaaacgcc atacgcatcc
     5341 gtttgtcgat acgccggaaa aggcgcgcgc ggtggtggag aaggtcaatc ggagccggca
     5401 ggaaaacggt cagcgtccga ttgcgtttgt cagtgtggtt gatgacgaaa tccgtcggat
     5461 tatcaaaggg gcggatgctt ttcagattaa tttctttgag acttttttgg gactgttgga
     5521 gaaagaactc aataccgaag ccacggcatc cgagcagggg catcacagta tcggtaatac
     5581 gaagcgttac gatgcgcgta tggaggcggt gaatttctca ttgaaccacg atgacggggt
     5641 cagcgataag aaccttcagg aagcggatgt aatcttgatg ggtgtatcgc gttcgggcaa
     5701 aacgccgacc tgcctttacc tcgccctgca atacggcatc cgtgcggcaa actatccgct
     5761 gattcccgac gatttggaat cggccgatct gccgcgtatg gtcaagcctt atagggataa
     5821 gctgttcggg ttgaccatcc agccggaacg tttgcaggcc atccgccaag agcgccgccc
     5881 gaattcaact tatgccaaaa tcgatacatg ccgcagcgag gtggcggacg cgcagagtat
     5941 gttcagacgg catggcattc cgtttgcgaa tacgacggat aaatcggttg aggaattggc
     6001 ggtacacatc cttcaggcgt gcaagctcaa acgcaggttt tgacgggctt tgattcggtt
     6061 tgaaggcgga actgccgtct gaaatcaggt ttcagacggc a
//
LOCUS       DAWFBO010000118         6087 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFBO010000118 DAWFBO010000000
VERSION     DAWFBO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.118"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            57..1610
                     /locus_tag="WGO87_001577"
     CDS             57..1610
                     /locus_tag="WGO87_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0392921.1"
                     /translation="MNRTYANFYGMLAAACRKNGNGTAVFEGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGKSRPVGETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1700..3727)
                     /locus_tag="WGO87_001578"
     CDS             complement(1700..3727)
                     /locus_tag="WGO87_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0392922.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLAVLVLTVPDQAQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEIGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            4016..6022
                     /locus_tag="WGO87_001579"
     CDS             4016..6022
                     /locus_tag="WGO87_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0392923.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         1554 a         1854 c         1423 g         1256 t
ORIGIN      
        1 cggcatattg ttttcaaatg agggcgttcg ccgtccgcaa ccataaagga agtttcatga
       61 accggactta tgccaatttc tacggaatgc tcgccgccgc ctgccgcaaa aacggcaacg
      121 gcacggcagt gttcgaaggc aaggaaaaaa ccgcctaccg cgcactcaag caggaagccg
      181 aagccgttgc ggcgtatctg caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg
      241 cggtttccaa ttccacagaa tttattaccg cctatttcgc cgtctccgcc atcggcgcgg
      301 tcgccgtacc gatgaacaca tttttgaaaa acagcgaata cgcgtatatc ctgaacgact
      361 gcaaggcgcg cttcctgttc gcctcggcgg gcctgtcaaa agaattggcg ggcttgaagg
      421 cgcaaacgcc cgtcgaaaaa atcatttgga cgggcaaaag ccggccggtc ggcgaaacgg
      481 cggaaggcga tgcctttttt gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc
      541 gacaaccccg gataaatgat ttggcacaca tcatctacac ctccggcacg acggggcatc
      601 ccaaaggcgc gctaatcagt tacgccaacc tgtttgccaa cctgaacggc atcgaacgca
      661 tctttaaaat ttccaaacgc gaccgcttta tcgttttcct gccgatgttc cacagcttca
      721 cgctgacggc tatggtgctg ctgccgattt atatggcgtg ttcgattatt ttggtcaaat
      781 ccgtttttcc gttttccaac gttttgaaac agacactgct caaacgcgcg accgtgtttt
      841 tgggcgtacc cgcgatttac accgcgatga gcaaggcgaa aatcccttgg tatttcagat
      901 ggttcaaccg catccgcctg tttatcagcg gcggtgcgcc tttggcggaa caaaccatcc
      961 tcgatttcaa agccaagttc ccccgcgcca aattgctgga aggctacgga ctgagcgaag
     1021 cctcacccgt cgtcgccgtc aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc
     1081 ccctgcccgg tttggaagtc aaagccgtcg atgaagaact ggtcgaagtg ccgcgcggcg
     1141 aagtgggcga actgattgtc aggggcggtt cggtgatgcg gggctacctc aatatgcctg
     1201 ccgccaccga tgaaaccatc gtcaacggct ggttgaaaac gggcgatttc gttaccatag
     1261 acgaagacgg ctttatcttt atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc
     1321 aaaatgtcta tccgcgcgag attgaagaag aaatctacaa actcgatgcc gtcgaagccg
     1381 ccgccgtcat cggcgtgaaa gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat
     1441 tgaaggaagg tatggatttg ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg
     1501 caaatttcaa aatccccaaa caaatccact ttaaagacgg gctgccgcgc aacgctacgg
     1561 gcaaggtatt gaaacgggtg ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc
     1621 gtccgaagcc ccgtccggca aaaaaaatgc ggtgaatctg cttcaccgca ttttttggca
     1681 aacgccggag ataaggcact tactctgcca attccacctg ctcgtgcgcc atcagttcct
     1741 gaccgacatc atccaacaac atcaaataac gttcgtagtt tttcaaaatg tcggcaatca
     1801 gctcgtcctt gttcatatac tgtacatcgt acccgacgcg cccgtcgaaa aaataagcgt
     1861 agggtttgta agttgtctga tgccggatat gcggcagctt gccgtcgtta atcaactggt
     1921 cggatacatc ctgcccgaca gacttaatcc cgtacataaa atcgcgcatc gtctctttcc
     1981 gaatgacgaa ctcgattgcg ggctcgtcct gatgaaacat cttatcgacc cggacgctca
     2041 agccgtattc ttccgaaagc tcccgttgca actcgtgcat agcgggcgat gcggtatgtt
     2101 tgaggaattt taaaatatcc tgctcctgcg tctggctcat tatctgcacc agccgttctt
     2161 tccacttgcc gcccgtccaa aatacactgg tagggttaac ccgggtctca aaatatttct
     2221 tatccgcact caatcctttc cacaggctga aacacattat cagcatcagc agggcaaacg
     2281 gcagggaaac aatcagggtc atagactgca ggttgccgag tccgcccgag cgcatcagca
     2341 aaacggcaac ggcagacatc agcacgcccc acataaccgc ctgccaccgt ggcgcgctca
     2401 agcccttgtc ccgagaggta atattgttca ggacataaat cccggaatcg gcagaagtta
     2461 caaaaaacag agaaatgacc agcaggctga cgatactcgt caattcgggc agagggaggt
     2521 aattaaagaa tttaaaaagc agcgtttccg gagaggaggt catcttttcg agcattcccc
     2581 ccgcaacccc gtcattcagc caaatcgccg tattgccgaa gacggtaaac cacaaaacgc
     2641 cgaacaggcc ggggatgagc aaaaccccga agacaaactc gcggatggtg cgcccctttg
     2701 aaatgcgcgc gataaacaaa cccacaaacg gcgcccaaga acaccaccac gcccaataaa
     2761 gcaccgtcca agattcaaac cacggcttgt gttcccgttc gtacgcataa gttttaaaac
     2821 tgaggcgcac cagatttccg aggtagttcc ctatgttgtc gccgaatgcc gacaacaggt
     2881 aaacagtggg acccgccgcc aaaacaaaaa acagcagcaa aaacgcaagg ctcaggttca
     2941 actcgctcaa caccttcacg cccttcccca cgccggatat tgccgaaacg acggcgaggg
     3001 acatgacggc ggcgataatc aaaacctgca cgctgaagct gttttcggca atccagccta
     3061 tttcctgcaa tccggcgccc agttgcgaag ccccgaaccc caatgtggtg atgatgccga
     3121 aaaaagtagc aagcaacgcc ataatatcaa tggcatcgcc gaaccttccg gaaatttttt
     3181 ctttcaacag ggggtaaaaa caagaacgca gggcaagcgg cagcttgtag cggaaaccga
     3241 aataagccaa agccaatgca atcgtaccgt acaccgacca agcgtgaacg ccccaatgga
     3301 acaccgtgtg cagcaatgcc tgctgctgcc tgtgttccgg cgtgccggcc gtaatgtccg
     3361 aaaaataatg catcaacggc tctgccacgc cgaaaaacat cagacccacg cccatcccgg
     3421 ccgcaaacag catcgccagc cacgacagga agccgaattc cggcacatct tcatcccgtc
     3481 cgagcctgat gtttcccaaa ctgctgaccg agagtatcag caggaaaccc agaaaaatgg
     3541 aaaacgttaa aacataaaac cagctgaact cggtaaaaat gacttctttt gcccgatcga
     3601 gccacatctg cgcctgatcc ggcacggtta aaaccaatac cgccaaaaca cacacaaaaa
     3661 acaaagtcgt caaaataacc atcggattaa atgacgttcg gcgttctata aattcagaca
     3721 gggacaaacc ttctcactcc tttgttaaaa acagacaaac ccggtcatcg ggcaaagcgg
     3781 tcaaaacctg ccggtaaaat accggcttcc ggatgacgaa atgcacaaac cggccgaaat
     3841 tgttataatc gaaaaaatta aaaatcaata cggattattc tatcagattg atttatcgat
     3901 atttattata tcattaatat agtttgattt caaaccggcg gaaaatgcgc cgcccaccgc
     3961 aggcgcgccc tccccgccat cggggcgttt aacgccggat tgccgatgcg gtacaatggc
     4021 tgatatgaag aaaattaccc ctcaaaacct acgccccctg ctttcggaaa gcttgggaca
     4081 taccgatttt gtcaacgtcc tcaacgcact gattaaattt ttgcgccgtg gcggcaaaaa
     4141 atgtgcgggg gaacgtttcg acctgattat cgacacattc aaacaagaca gggaattact
     4201 gtcccgcttc agccggtgtt tttacatctg gctcgcgcaa atacacattt atccggcact
     4261 catcaaactc ggcatcttct cgcgccacag ctttgcccgg gaaatgggca tacgcatcta
     4321 cgaacgcttc agcccgtcat ataaagattt tgccaacttg ggcgaagtct tcctttatct
     4381 tttccattcc gaaaacgacg acaaatggct gcaaacgctc aatatccgcc aatggctggt
     4441 tttatacgaa ctcatccgga gccacgccga gccgtccaaa ttgcagacgg cgggcatccg
     4501 ccttgccgat gcgcgtttgc gcgccatcga aatgctgtct gtctggacgg catccgaagc
     4561 catcgaaccc gacctcatcc gcatcgcccc gcgcctgctg gaagccgatt cttccttcgt
     4621 cgccctccaa cgcgaaaccg ccaaactggt cgaacactac cgcaacggca ccacgcctta
     4681 cgacaccgcc cacctcgaag tgatgttcga ccaatgtttc agccagattg actatttgcg
     4741 ccgcaaaggg acgggcgccg gctccggttc gtcggtcaaa gtcgcccacc tgctcgaacg
     4801 gctccggcag accgtagacc gtctgaagct gctcaccgac atccaaaccg gcgccggcaa
     4861 cagcaaccgc ctgaccatcg cgctgatgaa ctccctcatc tacgcggcgg tcgaacaata
     4921 cagcacccgc cacctgcgcc gcagcagcat ccgtatgctc gcccgcagca ttaccgaaaa
     4981 caaaagccac cacggcgaac actacatcac ccgcaaccgc aaagaatatt tcaaaatgtt
     5041 ctactcggcg gcaggcggcg gcatcatcat cgccctgatg gcgctgctca aaatccgcat
     5101 cggcacactc ggcctcagcc ccttcctgac ttccttgtcg gctgggttca actacggcat
     5161 cggctttatg atcatccata tgctgcactg caccgtcgcc accaaacagc ccgcgatgac
     5221 tgccgccagc tttgccgaac aggtcgatct caacgaaggc ggcaaagcgg tggacaacaa
     5281 actcgccaag ctcctcatcg acgtatgccg ttcccaaagt gtcgccgttt tcggcaacgt
     5341 ttccatcgcc atccttttgg cgtgcgccat atcgttcggc tatgcccatc tgtaccggca
     5401 gcccatactc gatgcccaca ccgccgccta ccagttcaaa tccatagaca tcatcgctta
     5461 cccgacgctg tggtatgccg ccattgcagg tctatggctg ttctgctccg gcatcatcgc
     5521 aggttttttc gacaaccgcg ccgactacct caacctgcgc caacgcctgc ccttcaaccc
     5581 cttgctgcgt aaaatcatgc gccccgggcc ccgccgcgtc ctcgccgcct acatccacaa
     5641 acactacggc tcgttggcag gcaacttcat cttcgggatg ctcttgggta tgaccggcta
     5701 tttcggacac ctcctcgggc tgccgctgga catccgccac gtcgcctttt cctccgccaa
     5761 cctcggctat gccgccgtca gcggcaacgt cggtttgggc acgttcgtac tcggcatttt
     5821 cagcgtcctc gccatcggcc tggtcaacct ctgcgtcagc ttcagcctcg ccctcttcgt
     5881 cgccctgcgc tcgcgcggca cgaaaatcgg cagcatccgc aatctgatta agagtttttg
     5941 gaatcagatt aaaagcaatc cctgcatact tttcctcccg cccgccaaag aacagggaca
     6001 tcctccttcg gacaagcctt gaccggcaat gccgtctgaa gcgggattcg ccccgaatac
     6061 cgccctgatg cgggaaatcc ccataaa
//
LOCUS       DAWFBO010000119         6023 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFBO010000119 DAWFBO010000000
VERSION     DAWFBO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.119"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WGO87_001580"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WGO87_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0392924.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WGO87_001581"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WGO87_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0392925.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WGO87_001582"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WGO87_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0392926.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIASITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1357 a         1708 c         1619 g         1339 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcaccct ccgccgcata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgct tgtaacgcag gccgaccaag
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaatg cggattggac ggcggattca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcgaacg accaaacgcc cgtgccgctt
      481 ttgaacagcc cacccaattc ggcagaaccc gtaccgacgg ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcagcacgga tatcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagcttc
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaggg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcgtaa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagccg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcgtgcc aacccaaatc gacggcgcgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg tgggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg tccgaaccgg tgatgcccgc ttcggcagcg
     1561 tgttttactg ccatttcgtg ttcgtgcgcg gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa caccgacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcaaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaattcgata
     1861 atcaaaatcg catttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccattcgaag
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cgcttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccagcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattattg cggtcttgca ggttgatgct caggcccgaa
     2701 ccattgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg caggcgcata
     3061 aacaggaaca cgcccacaac cgccaaaccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactattgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaaag cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtgt gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgca ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacaaata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcacccg gataagtggc gtgcaaagta atggtcgggg cggcgacaga cggatattgc
     4621 gaaacaggca ggcttttgat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcaaaaa tagggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgccgga ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ctggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcaacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacaaca aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gtcggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc aacagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt aatgccggca gatttgattg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtatcgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcgctt tccagacctg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgtcc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg atgatgccgc cgacttgggc gcggacatcg gcggtacgca
     5761 gcgattccaa acgccccggc aactcgacgg tcaacgcgac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggcgca ggggcttccc gaccagcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc att
//
LOCUS       DAWFBO010000120         5996 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFBO010000120 DAWFBO010000000
VERSION     DAWFBO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.120"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1..720)
                     /locus_tag="WGO87_001583"
                     /pseudo
     CDS             complement(1..720)
                     /locus_tag="WGO87_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694185.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1842..2087)
                     /locus_tag="WGO87_001584"
     CDS             complement(1842..2087)
                     /locus_tag="WGO87_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392927.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            2199..2627
                     /locus_tag="WGO87_001585"
     CDS             2199..2627
                     /locus_tag="WGO87_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0392928.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            2639..3115
                     /locus_tag="WGO87_001586"
     CDS             2639..3115
                     /locus_tag="WGO87_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0392929.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(3465..3953)
                     /locus_tag="WGO87_001587"
     CDS             complement(3465..3953)
                     /locus_tag="WGO87_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0392930.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(4021..5076)
                     /gene="aroG"
                     /locus_tag="WGO87_001588"
     CDS             complement(4021..5076)
                     /gene="aroG"
                     /locus_tag="WGO87_001588"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0392931.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWDATEE
                     LLALLAGANKKRMARAG"
     gene            complement(5238..5465)
                     /locus_tag="WGO87_001589"
     CDS             complement(5238..5465)
                     /locus_tag="WGO87_001589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392932.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            5401..5633
                     /locus_tag="WGO87_001590"
                     /pseudo
     CDS             5401..5633
                     /locus_tag="WGO87_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5692..>5996
                     /locus_tag="WGO87_001591"
     CDS             5692..>5996
                     /locus_tag="WGO87_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392933.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKS"
BASE COUNT         1495 a         1462 c         1387 g         1652 t
ORIGIN      
        1 tttattgaag atgttgttga gactagtctg tgatgtatta ctatgaggag tgagtacgga
       61 atcttctacc aatttattaa ttaaatcggg catcgtctgt ttgagcgtac taaattttgt
      121 tccaaactgt gctatatacg cctcctcagt ccgtttttta ttttctgccc aagcttccgg
      181 tcttgttttg tataaatcct gtaattgact tttaaccgcc tgctttaaat cagccccata
      241 cttctctgta atatattttt tgacaacggg catattatcg acggcgagga tgccgctatt
      301 gtaattttta ccttggatat tgatttcgtc atacccatat tcggatgaat tttgatcggt
      361 tttaccgctg gaaaaataat gggaagtgcc gggtttcggc ttgtctgaaa atgaaaaatc
      421 ggcatcgtcg ccgattttgg tagaacgagt atttgaacca agctgagctc tgaacatacc
      481 gaagcaatca aaagatatgc tgcacgggcc gcgccaaacg tgggaatttc tgtcttccgc
      541 cgcttctaaa ataaatttgt ttttaatctc ggcttctttg acgggatcgt acgcatccga
      601 acctcaaaca tatggcgtgc aaaacagcga taaaacagat aatgttaata gattaacccg
      661 accgcttgct tgcttgcttg cttgcttgct tgcttgcttg ctttcagtgt catgagaaat
      721 tttcctttgt caagtgttaa aattataatg attatatact acattatact acatcgcaat
      781 gaaaaatcag gaaaagcaaa aaaccctccg ccgtcattcc cgcgaaagcg ggaatctaga
      841 attttaatgc cgcaagaatt tatcaaggat ggtcggggtt taaaggtctg gattcccgcc
      901 tgcgcgggaa tgacggttta gaaatcaccc gaaacaagaa aaagaaccga aaccgaacaa
      961 gccggattcc cgcctgcgcg ggaatgacgg gatttcaggt tactgttttt gattttctgt
     1021 tttgtggaaa taacgggatt ttggatgatg tgcatttatc gagaaaacta aaaaccctcc
     1081 gccgtcattc ccgcgaaagc gggaatctag acctttcggt gcggaaactt atcggataaa
     1141 acggttttcc ctgcatcctg gattcccact ttcgtgcgaa tgacgggata taggtttcac
     1201 tacggacgtg ttcagattac cgcctgcgcg ggaaggacgg gatttcaggt tgctgttttt
     1261 gattttctgt ttttgtggga ataacgggac tttggatgat gtgcatttat cgggaaaaca
     1321 aaaaaccctc cgccgtcatt cccgcgaaag cgggaatcta gaattttaat gcggcaagaa
     1381 tttatcaaga aaaaccgaag cttaaagacc tagattcccg cctgcgcggg aatgacggtt
     1441 tagaaatcac ccgaaacgag aaaaagaacc gaaaccgaac aagccggatt cccgcacagg
     1501 cgggaaggac ggaatttcag attgttgttt ttgattttct gtttttgtgg gaataacggg
     1561 actttggatg atgtgcattt atcgagaaaa ctaaaaaccc tccgccgtca ttcccgcgaa
     1621 agcgggaatc tagaatttta atgcggcaaa aatttatcgg aaaaaaccga agtttaaaga
     1681 cctagattcc cgcctgcgcg ggaatgacgg ctcaaaagtt acgaaacgaa aaataaccga
     1741 aaccaaacaa gtcggattcc cgcgcgggcg ggaatgacga taagcgtaat ttcaatctgc
     1801 catattgtct tttcacttgc ctgaaaacag atttcagcca cctatccctg catccggcgg
     1861 ctgatgtagt cggcaatcaa atcccgcttt tcccaaaaaa acttctgcat tttttgttcg
     1921 gaatacagtt tgcggaccag agtgggcaaa ccgctgtgga cgatggcggc gatttctttg
     1981 ttgccttttt cttcctgcat tgccgtctga aaatccatac ccctgccttt cagatattct
     2041 gcaaggcggt tgcggatgtg ggaattgagt tcgatggatt tgagcatttt gcctccgttt
     2101 tgcggtaagg atttattcga tttgtttatc cgcctgattt tatcacgttt cccaagcggt
     2161 ttgcctgtac aataccgctt ttcggcaggg tgtgggcgat gatgttttct tggttcaagc
     2221 tgtttcactt gttttttgtc atttcgtggt ttgcagggct gttttacctg ccgaggattt
     2281 tcgtcaatat ggcgatgatt gatgtgccgc gcggcaatcc cgagtatgtg cgtctgtcgg
     2341 gcatggcggt gcggctgtac cgttttatgt cgccgttggg cttcggcgcg gtcgtgttcg
     2401 gcgcggcgat accgtttgcc gccggctggt ggggcagcgg ctgggtacac gtcaaactgt
     2461 gtttgggctt gatgctcttg gcttaccagt tgtattgcgg cgtgctgctg cgccgttttc
     2521 aggattacag caatgctttt tcacaccgct ggtaccgcgt gttcaacgaa atccccgtgc
     2581 tgctgatggt tgccgcgctg tatctggtcg tgttcaaacc gttttgacgg agcatcttat
     2641 gccgatagaa atcgaacgcc gttttttgat tggaaacgac aactggcggc aatacgccga
     2701 cgaacctctg ctgttgaggc agggttatct gtctgttgaa aaagaacgca ccgtccgtgt
     2761 ccgtattgcc ggacaacggg cgtggctgac gctgaaaggc tatatttcgg aactcagccg
     2821 cagtgagttt gaatacgaaa tcccgcttgc cgatgcggaa aagatgatgg aaacgatgtg
     2881 tccgtttaag atggaaaaat ggcgttatcc ggtcagatgg ggcggcagcc tgtttgaaat
     2941 cgatgttttt cttggcgata atgcgccttt ggtcgtcgcg gaaatcgagt tgcccgacga
     3001 gaatgccgat tttgaccgtc cggactggct gggacgggaa atcactgccg acggtatgtt
     3061 taccaatgcc tatttgagca ggcatccgtt ctcaagctgg aaaaatgccg tctgaagcgg
     3121 cttcagacgg cattctccga caaccatcgc aaatccaaag acagcctcta tatccttatt
     3181 tctgctttct ttgttccatt attcccaata aatccccata acctaaaatc tcgtcattcc
     3241 cgctcaggcg ggaatccggt tctttaaact tcggtttttt ccgataaatt gctttagctt
     3301 ttcgtttccg gattcccgct tttgcgggaa tgacgatgga agattgttgt tatttcggat
     3361 aaattaccct aactttaaaa tcccggattc ccatcttcgg gggaataaca atattttcta
     3421 tttcagccca tcatttgcgg gggattttat agagagttta tatttcaata tctcaaataa
     3481 tgcacaaaag catagcgcgt gcctttgctg ctgacacggc gttccgtccg ctctgcttct
     3541 ttccaatgcg tccggtctat tgcggggaaa aatgcatctc cttccacaga caaatccact
     3601 tcggttatcc gcaaatcggt cgccaatggc atcgcttgtc cgtatatctg cgcgccgccc
     3661 ataatgacgg cttcttccgc gcctgcgcac aatgccaatg ccgcctccaa acttgccgcc
     3721 gtttccgcgc ctgccgcgca ataatccgcc tgccggctga tgacgatgtt cctccgtcca
     3781 ggcaggggtt tgacgggcag ggattcccac gttttccgcc ccataatgac gggtttgccc
     3841 aaggtatagg ctttgaaaaa tgcgaaatct tcggggatgt gccaaggcat agcattgccc
     3901 gccccgatgc acaggttttc cgcacacgcc gcaattaggg ttattttgag catatttcct
     3961 ccacaaaaat gccgtctgaa cgcttcagac ggcattttat gacattctgc gtcaaaaatc
     4021 tcaaccggcg cgcgccatac gttttttgtt tgcacctgcc aacaatgcca acagttcttc
     4081 agtcgcgtcc caaccaatac acgcatcggt aatgcttttg ccgtacactt ccggcttgtc
     4141 ctgcctgcct tcgaccaaat ggctttccac catcacgccc ataatattgc cgccgtcctg
     4201 ttccaattgg gcggcaatgt cttgcgccac ttccatctgc cgggtgtaat ccttgcggct
     4261 gttggcgtgg ctgcaatcta tcatcagctt atcggttacc cctgccgcac gcagttgttc
     4321 cgccgcctcg ctgacgtgtc ccgcatcata attcggctct tttccgccgc gcaaaatgac
     4381 atgacagtcg ggattgccgc cggtatggac aatggcggaa tgtccggcct tggttacaga
     4441 caagaaatga tgcgaatggc tcgccgcacc gattgcgtcg atggcaatct tcaaattgcc
     4501 gtccgtaccg tttttaaagc cgacggggca ggacagcccg cttgccaatt cgcggtgaac
     4561 ttggctttcg gtcgtccgcg caccgattgc cccccaagaa atcaagtccg cataatattg
     4621 cggcgtaatc atatccaaaa actcggtaga ggcaggcata cccatattgt tcagcgacaa
     4681 caacaggctg cgcgcctgac gcaaaccgaa attgatgtca aacgtaccgt ccaaatgcgg
     4741 gtcgttaatc aaacctttcc aacccaccgt cgtcctcggc ttctcgaaat aaacgcgcat
     4801 cacaatcaaa agctcgtttt catactgctt gcggagtttc aacaaacgct ccgcatattc
     4861 caacgccgct ttcggatcgt gaatcgaaca cggcccgata ataaccaaca gccgcttgtc
     4921 cctgccgtga accaaatcgg aaatttcctg acgggtgcgg tgaaccaagc ccgaagcctc
     4981 tttggaaatc ggcagctcgt aaagatgggc aatcggcggc aacaactctt taacttcttt
     5041 aatcttaata tcgtctgtgg ggtaatggtg tgtcatcttg catctttcct gatggttttt
     5101 tatacgcggc ttaggttaca tcgtttacct tgcacaaaca agtgcaacat attactacaa
     5161 agctatttta tttcaaaata actgatatat cttttttcat agattttaaa tttcatattt
     5221 ttaatatttt aggaatttta ttccaaagaa aaagaaacag attgataaaa acaatacccg
     5281 ccattcattc ggacaaaacc gcacggaaca tccgttttcc catttccacg acaatccggt
     5341 acagaacacc ccgctgccga ttccgcctgc acatccggca aaatttcata cgcaaggaaa
     5401 atgttgaaaa caaaaagaac taattgcttg aaaaaagatt taattggaat gcgggtattt
     5461 cccacacttt ctccatttat tcatcctcat tataggaaga aaaacatgaa aaaaccctgc
     5521 ttgcactctc acttgcagcc gtgtccgccg ttacggtagc cgatattcct gagtctaacg
     5581 gcaagggcaa agccatcatc ggcgataagg aaaaacaccg ttaccaagca tgaaaacctt
     5641 gttttgaaag tctatggcga aatccgccaa gatttggggg acggatgatc cctgacccct
     5701 tgggcggatg catatgcaga tttgcgcggc aaaaccaaag tgatgacgac ccagatgtgt
     5761 gcttcccgcg atgtcagcaa aagcgccaaa ggttggagtg tcggtatcgg tctgaatgta
     5821 ggcaaacagt tgaccgacag cgtcggtctc gagtttgatc catactaccg tcacaaaaca
     5881 atctgcaaac cccgtgagat tgttttggac ggcgacaaaa ccaaaatggg ccgctccaaa
     5941 tccaacgagt acggcttccg cgtaaccgca acgttctata gtcaattaaa atcaaa
//
LOCUS       DAWFBO010000121         5989 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFBO010000121 DAWFBO010000000
VERSION     DAWFBO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.121"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            59..505
                     /gene="yciA"
                     /locus_tag="WGO87_001592"
     CDS             59..505
                     /gene="yciA"
                     /locus_tag="WGO87_001592"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0392934.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
     gene            complement(556..1377)
                     /locus_tag="WGO87_001593"
     CDS             complement(556..1377)
                     /locus_tag="WGO87_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0392935.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1496..1954
                     /locus_tag="WGO87_001594"
     CDS             1496..1954
                     /locus_tag="WGO87_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0392936.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2118..3233)
                     /gene="lst"
                     /locus_tag="WGO87_001595"
     CDS             complement(2118..3233)
                     /gene="lst"
                     /locus_tag="WGO87_001595"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0392937.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNHGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFKQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3334..3438
                     /locus_tag="WGO87_001596"
                     /pseudo
     CDS             3334..3438
                     /locus_tag="WGO87_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3666..5891
                     /locus_tag="WGO87_001597"
     CDS             3666..5891
                     /locus_tag="WGO87_001597"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0392938.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTDGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1610 a         1754 c         1330 g         1295 t
ORIGIN      
        1 aacgccgtac tggtttaaat ttaatccact atatatttca ataaaacaag gaaactttat
       61 gcagcacgaa gaaggcaacc gccaacgccc tcaaggcgaa ctgctcctgc gtaccgtcgc
      121 tatgccgcgc gataccaatc ccaaccaaga cattttcggc ggctggatta tgtcgcagat
      181 ggatttgggc ggcggcatat tggcggcgga aatcgcgcgg ggacgcatcg ttaccgtcgc
      241 cgttcaggaa atgaacttta tccgcccggt caaggtcggc aacgtcgtct gctgctacgg
      301 gcattgcgtc cgcgtgggca atacttccct ccagcttaaa atcgaagtct gggtgaaaac
      361 tttgatgaac gattgcgtta ccgaagaccg ctacctcgta accgaagccg tgttcactta
      421 tgtcgccatc gatgcggaag gcaatccgcg cccgattccg aaagaaggca accccaaatt
      481 ggcgggctta ttgcctactc cgtaaaaatg cccgtaaaaa tgccgtctga aactgtcttc
      541 agacggcatt ctgcatcatt tgaaccaaga cctgatgcgg gaaaacagcg acatactttg
      601 tttttcaaag tcttcccgca cttcgggcac ggcttttttt atcactttca ttcctgcaaa
      661 agtctgcgcc acgggcataa tttcaatcgt gttcacattc atatgcgccg gccggcggta
      721 cagccacaat gcggtttccg caatatcttc ggggcggata aattccacac cctcatacac
      781 gcctgctgcc ttctcgtcat cgcctttgaa gcgcacgttg gaaaactccg tattgccgca
      841 caaacccggc tcgatattgg taacgcggat gttcttatcc gccaactccg cgcgcaaatt
      901 caggctgaac tggcgcacaa acgccttggt cgccccgtaa acgttgctgc cggcataagc
      961 ataattgcct gcaatcgaac ccaaattcat cacataaccg ccgccgcgtt ccaccatttg
     1021 cggcaaaatt ttgcgcgtca ggaacgtcaa acccaaaaca ttggtttgaa tcatcgtttc
     1081 ccaatcttca aaatccgcct tgtccgccgt gtccagaccc aaagccagcc cggcattgtt
     1141 gatgaggcag tcgatgtcgg aaaattcatc ggggatgccg tttaaggcgt tttccaccga
     1201 ctcgcgtcgc gacacgtcca tttccaaagg gtaaaacaaa gcacccaatt catccgccaa
     1261 ggcctgaagc ctgtccgcac ggcgcgccgc accgataacg cggtatcccg ccccgacaaa
     1321 cgcacggcac atcgcttcgc cgaatcctgc cgaagcaccg gtaattaaaa cagccattgt
     1381 ttttcctttc tttctcgttt cccaaacctg tttcaggtat cataacgctc ttcagacggc
     1441 atcgcgccgg ctgaaaatga caacaaccat accaccacga caggacacat tcaatatgaa
     1501 aacccaaatc agccttgctg ctgccgccat cacactgctc ctttccgcct gcggaggcag
     1561 cggaatacct tcccaaccca aaggcgaaat ttccgaaaac cgcaccgccg cgttcaaatc
     1621 catgatgccc gaattcacac gcatgggcaa aatggtcaaa gacgaagaac cttacgatgt
     1681 cgaaaaattc aaacaggctg ccgcggcgtt tgccgacagc agcaagaaac cgttcacact
     1741 ttttgagtcc gatccgcaag gcaacggccg cgccctgccc gccgtttggt cggatggtgc
     1801 aaaatttgaa gccgaaaaaa caaaattcgc cgccgccgtc gaaaaactca acgccgcagc
     1861 acaaaccggc aaactggatg aaatcaaagc cgcctacggc gaaaccggcg caagctgcaa
     1921 atcctgccac gacagtttcc gcgcgcccga ataacatcca aaacaaaaca atgccgtctg
     1981 aagccttcag acggcatttt ctttgccatt tcagcacacg caaagcaatc agaaattatc
     2041 ttcaattcaa aaaatacgcc atatcctgat ttttgcctta cttactccgc cattttctat
     2101 tttgatttgc tatgcgatta atctttatcg tcaaatgtca aaatcgggat gccggattgg
     2161 gtaaacaggg catacaccgg cttgagccaa taatcttccg gaagggaagc cggtttcaat
     2221 gcgtaaacgt gcacattggg aaaatccttc atcgtcaacg ccgcgccgct gaaaaaggta
     2281 taaatttcat acctcgtatg cgggtttttc ttaatctcgc gcaaaatata gtcttcgatg
     2341 acatagggcg aatttaatgt ggttacgccg gaaagcccgt aggtttggcg ggggtgcggt
     2401 gcgacatatt gtatgttgaa gttttttgcc gccttttccg aaatttcctt catctccttg
     2461 tcgggcgaac ccaaaagtat ccgcaccgtg ccgcccgttt cgtcccccgc cttcagttcg
     2521 gacgcatcga acagcggcag gtaagtcatc ttgcggcggc cgtcgtccat aatgtttttc
     2581 aaacccttga atatcgtgta atgctcgtcg gaagcattac gggttttggc gatgctccaa
     2641 tctccgatca tcatccgggc aaaattccgc ttgatcgtcc cgtttacaga aaactcatcg
     2701 cccaaatagc tgctgctttg aattaaattg cctgtcccgt cgtcaaaggt tttgatttcc
     2761 gcatccgggt aagtgctcaa aaaggcggca atgctgactt tttccaaact tgccaaataa
     2821 atccgcttga ctttcggcag cagcatcgac tttaccttca gctccgccat cgtcggaatg
     2881 aaattaaacg atttgttcaa accgtagggc aggtggaaaa aatacgcccg ctccgcctta
     2941 tcctttatct gcttgaaata ataatcgtat ttttcattcc tgttttcaga catcagcacc
     3001 acataaaacc gctcccccgg atgctgcgcc ataatccttt ccgccacctt catctgcaat
     3061 atggtatagc agaaaatcag attgaccggt tccccctctt cattgaagag tttgtccttc
     3121 agcagggaaa ccgcattcct ttccccatga tttacccggt caaatgtata aaatatcccg
     3181 aaacaaaaaa caatcaaaca caacacggtc aaacaagcct ttttcaagcc catatcccta
     3241 aaactccatt ccgacaaatt gaacatacgc cccgagtgtc ccgggacgga aaaatgcgga
     3301 attttagcag atattccgac agttaccgaa tattatagtg gtttaaattt aaatcaggac
     3361 aaggcgacga agccgcagac agtacaagca gtacgacaag gcaaggcaac gctgtactgg
     3421 tttaaattta aaccactata aaatcaattt caccgaaaat atgccgccgc acatcaggca
     3481 cagcacctcc gaccgccacc aaacgcggga aaaccatttc ccccttcctt tcacgctgcc
     3541 gcgttcaaac acgccaagat cgaacaaagc gcgaacaaag ccgccaaaat atgccataat
     3601 agccgcgcac attccccgcg caatgtgttc caaaagaaaa aaccaaccta caagagaata
     3661 atcctatgac tcaaaaatcc accattgttt atacccatac cgacgaagcc cccgcgctgg
     3721 cgacccaatc gctgctgccg attgtgcagg cttttgcccg ccacgccgat attgatgtca
     3781 aaactgccga catttccctg tccggccgta ttttggcggc attccccgaa tatctgaccg
     3841 aagcgcaacg cgtacccgac gcgcttgccg aattgggcga actggtaaaa caacccgatg
     3901 caaacgtaat caaactgccg aacatcagcg catccgtacc gcaactgacc gccgcgatta
     3961 aagaattgca gtctaaaggc tttgccgttc ccgattatcc tgccgacccg caaaccgatg
     4021 aagaaaaagc cgtgcgcgaa cgctacgacc gcatcaaagg cagcgcggta aaccctgtcc
     4081 tgcgtgaagg caactccgac cgtcgcgccc ccaaagcggt gaaaaacttt gccaaaaaaa
     4141 atccgcacag catgggcgca tggacaaaag actccaaaac ccacgttgcc accatgcaaa
     4201 gcggcgactt tttccataac gaacaatccg ttaccgtacc tgaagcgact tccgtatcca
     4261 tcgtgttcac cgacaaacaa ggcaataaaa aagagctgcg cgcgcctgtt gccctgaaag
     4321 ccggcgaaat catcgacgca accgtgatga gcaaaaaagc cctgctcgcc ttccttgccg
     4381 aacaagtgaa agacgcgaag gcaaaaggcg tattgttctc actgcatatg aaagccacga
     4441 tgatgaaagt gtccgacccg attatcttcg gacacgccgt caaagtgttc ttcgcgcctg
     4501 tatttgaaaa attcggcggc aaactggctg ccgcaggtgt caacgtcaac aacggcttcg
     4561 gcaacctgct ggccaatctg gacaaactgg atgcggacac ccgcgccgcc gtcgaagctg
     4621 aaatcgccgc cgtttacgcc gccaaccctg atttggcgat ggtcgattcc gataaaggca
     4681 ttaccaacct gcacgttccc agcgacgtca tcgtcgatgc ctctatgcct gcgatgattc
     4741 gcaattccgg ccggatgtgg gacaaagacg gcaaagcgca agacaccaaa gccgtcattc
     4801 ccgacagcag ctatgccggt gtttaccaag caaccatcga tttctgccgc gaacacggcg
     4861 cattcgatcc gacaactatg ggtaccgtgc ccaacgtcgg tctgatggcg caggcggccg
     4921 aagaatacgg ttcgcacaat aaaacttttg aaatcgaagc cgacggtcaa gtccaagtca
     4981 ttgatgcggc aggaaaagtc ctaatgcagc acgacgttga agccggcgac atctggcgta
     5041 tgtgccaaac caaagacgct ccggttaaag actgggtaca acttgccgtc aaccgcgccc
     5101 gtctgagcaa cacgcctgcc gtgttctggc tcgacgaaaa ccgtccgcac gacaagagcc
     5161 tgctcgccaa ggttaaagct taccttgccg aactggatac cgacggcctc gacatccgcg
     5221 tcctcgcgcc tgaagaagcc gccaagttca gcttgggccg tctgaaaaac ggcgaagaca
     5281 ccatctccgt aaccggtaat gtcttgcgcg actacctgac cgacctgttc ccgattttgg
     5341 aactcggcac aagcgcgaaa atgctgtcta tcgttccatt gatgaatggc ggcggtatgt
     5401 ttgaaaccgg tgcgggcggt tctgcaccga aacatgttca acaattcctc gaagaaaacc
     5461 atttgcgctg ggactcgctg ggcgaattcc tcgcactcgc cgtatcgttt gaacatttgg
     5521 cgcaaaaaac cggcaatgcc aaagcccaag tcctcgccga cactttggat gcagccaccg
     5581 aaaaactgct gttgaacgac aaatcgccta aacgtaaagc cggcgaactc gacaaccgcg
     5641 gcagccattt ctacctcacc ctctactggg cgcaagaatt ggcggcgcaa gacaaagatg
     5701 ccgaactgaa agccgcattt acgccattgg cagccgcttt gaccgccgac gaagcgaaaa
     5761 tcgttgccga gctttccgcc gtacaaggca aagcggccga catcggcggc tactacgccg
     5821 ccaatcctga aaaagccgca caagtgatgc gtccgagcgt aacctttaat caggcactaa
     5881 acgccttata agcacaaagg taaaaatgcc gtctgaacat tcgtcgttca gacggcatta
     5941 gttatcccta tctgcctgat tataattaaa cgccgcaccc attggatat
//
LOCUS       DAWFBO010000122         5971 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFBO010000122 DAWFBO010000000
VERSION     DAWFBO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.122"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(40..963)
                     /gene="truB"
                     /locus_tag="WGO87_001598"
     CDS             complement(40..963)
                     /gene="truB"
                     /locus_tag="WGO87_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0392939.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYAIDI
                     AEFDSPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFDEDLTSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAVSAA"
     gene            complement(996..1367)
                     /gene="rbfA"
                     /locus_tag="WGO87_001599"
     CDS             complement(996..1367)
                     /gene="rbfA"
                     /locus_tag="WGO87_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0392940.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            1558..2802
                     /gene="clpX"
                     /locus_tag="WGO87_001600"
     CDS             1558..2802
                     /gene="clpX"
                     /locus_tag="WGO87_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0392941.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIAWQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGKEPELVFES"
     gene            complement(2867..4060)
                     /locus_tag="WGO87_001601"
     CDS             complement(2867..4060)
                     /locus_tag="WGO87_001601"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HEZ0392942.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVCSQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            4464..4964
                     /locus_tag="WGO87_001602"
     CDS             4464..4964
                     /locus_tag="WGO87_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392943.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHPLPL"
     gene            5049..5603
                     /locus_tag="WGO87_001603"
     CDS             5049..5603
                     /locus_tag="WGO87_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392944.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            5617..5817
                     /locus_tag="WGO87_001604"
     CDS             5617..5817
                     /locus_tag="WGO87_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392945.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
BASE COUNT         1414 a         1605 c         1624 g         1328 t
ORIGIN      
        1 acacattatt caaacacagc ccgtattttt aaccgccgtt caggcggcag ataccgccgt
       61 gttcatcagg cgcaaggctt tcagacggca tatttctttt tgatattccg ccaggccgac
      121 aaagcggccg ttttccgtgt aaacgcgtac cggcgtatcg gaagtcaggt cttcatcgaa
      181 acgcggccgt tgcccgcatt ggagcatatg gacggcataa tcgtttaaaa cggtttgggg
      241 aaaatgggca accaatacgt cgcagggcag cagcaggctg tcgcgttctg tttcgtccaa
      301 gtttgccaag gcttcgagcg tgtggctttg ggcgatggta aagccggcgg tttcggtgcg
      361 gcgcagggcg gtcaggtggg cgaacgtgcc gatgtgtttg gcgatgtctt cgctgagggt
      421 gcggatatag gtgcctttgc tgcaacgcac gtcgatgacg gctttgggtg agtcaaactc
      481 ggcaatatcg atggcgtaaa cggtaatatc gcgcggcttg cgttcgatga cgatgccttt
      541 gcgggcgtat tcgtacagcg gtttgccttc gtgcttgagg gcggaaaaca tcggcggcac
      601 ttggcggatg ttgcctgtca gggcttggca ggccgtctga aattcggcta aggaaatatc
      661 ggcgcgtgcg gcagcgatga tttcgccttc ggcatcaccc gtgctgctgg cttcgccgag
      721 tttcagcgtg gcggtgtagg ctttgtcggc atccagcagg tattgggcga acttggtcgc
      781 ttcgccgaag cagacgggca aaagcccggt tgccaaaggg tcgagcacgc cggtatgtcc
      841 ggctttttcg gcacggaaca aacgccgcgc tttttgcagc gcggtgttgc tggaaaggcc
      901 ttcgggtttg tccaagagca aaacaccgtt gacggcacgt ttttggggtt tgcctgtatt
      961 catggttcga tgttcggacg gcatgggcat atccgtcagt cttcaaccgg tttttccgcc
     1021 gctacttggt cgataagggc ggacaggttc aaaccgcgtt ccaaggattc gtcgtatttg
     1081 aaatgcagtt cggggatttt gaacagcttg atgcgtttgg ccaattcgct gcggaggtgt
     1141 ccgcgcgcgt gttccagcac ttcttccgta atttcgcgcg cgtcttggtt taaaatggtg
     1201 tagaacacgg tggcgtggct gtaatcgcgg gtaacttcga cttcgttgac ggtaatgaag
     1261 ccggcgcgcg ggtctttcag tccggtacgg acgagttcgg caagctcgcg cataatttgt
     1321 tctttgacac ggtcttggcg ggcatagccg cgttggggtt ttctcatgaa tatcctcaga
     1381 tttcagattt ggtttcagac ggcatttttg ccgccggggc ggttgcgaaa gggcgtatta
     1441 tagcctaatc ggcgggattt taaggtaata cattgataag aacggatata gttattatac
     1501 ttggcaaccc gaacggcacg tgccgttttc tgtaacgcat acattgtaaa gaaagccatg
     1561 tccaacgaaa accgtacctg ttccttttgc ggaaaatcca aatcacacgt caaacattta
     1621 attgagggcg aaaacgcctt tatctgtgac gaatgcgtct caaactgtat tgaaatattg
     1681 catgaagacg gaaatgacgg cacgccttcg gaaagtgccg gaggggagcc ggaagaatcc
     1741 ggcaagctgc ccacgcccgc cgaaatcgtc gccaacctca acgaccacgt tatcggtcag
     1801 gaacaagcca agaaagcctt ggcggtgtcg gtgtacaacc attacaaacg cctgcgccac
     1861 ccgaaagccg gagctaatgt cgaattgtcg aaatccaaca tcctgcttat cggcccgacc
     1921 ggatcgggta aaaccctgct ggcgcaatct ttggcgcgca aactggatgt gccgttcgtg
     1981 atggcagatg caaccacgct gaccgaagca ggttatgtcg gcgaagatgt cgaacagatt
     2041 attaccaaac tgttgggcaa atgtgatttc gatgtcgaaa aagcccaacg cggcatcgtc
     2101 tatatcgacg aaatcgataa aatttcacgc aaaagcgaca atccgtccat cacgcgtgac
     2161 gtgtccggcg aaggcgtgca acaagccttg ctgaaactga ttgaaggtac ggtggccagc
     2221 gttccgcccc aaggcggccg caagcatccg aatcaggaat ttatcaacgt cgataccacc
     2281 aacatcctgt ttatctgcgg cggcgcgttt gcaggcttgg aaaaagtcat ccgccagcgt
     2341 actgaaaaag gcggtatcgg tttcggtgcg tccgttcaca gcaaggacga aaatgccgac
     2401 attaccaagc tgttcggcat cgtcgaaccg gaagatttaa tcaaattcgg tctgattccg
     2461 gaattaatcg gacgtttgcc tgtgattgca acgttagaag aactggatga ggacgcgctg
     2521 attaatattt tgaccgagcc gaaaaacgct ttggtcaagc agtatcaagc cttgttcggt
     2581 atggaaaatg tcgagttgga atttgaagaa ggcgcattgc gttccattgc gtggcaggca
     2641 atggagcgca aaaccggcgc gcgcggcctg cgttccatcg tcgaacgctg cctgctcgat
     2701 acgatgtacc gcctgcctga tttgcaaggc ttgaaaaaag tggtggtcgg taaggcagtc
     2761 atcgaagagg gcaaggaacc ggaattggtg ttcgagtcct gatggcaaac aaaaccgctt
     2821 cggaacgttg aaccacgttt ccgaagcggt tttttgtttt tgatgcctag gggcggtttg
     2881 cggcggcaaa ttccgtcagc acgtgttcca aacgccgcaa accttccgcc gtatcctcat
     2941 cgtccaacag cagcgaaggc gcgaaacgca atacgtccgc acccgcaacc aaaatcatca
     3001 cgccgtgttt caaggcggcg gcggttattt cggatgcgcg tccgcgataa ggcgcgtcca
     3061 acacgcagcc gagcagcagc cccatgccgc gaacttgctt aaacacgccc gtttgttctc
     3121 ccaaagaccg caatgccgtc tgaagtttct gcccctgact gcagacgttg tgcaaggttt
     3181 ccggcgcgtt gatgatgtcg aaggcgcggc tgccgaccgc acacgccatc gggttgccgc
     3241 cgaaagtcga gccatgcgtg cccggttgga aggcggcggc aatcgtttct gtcgccagca
     3301 tcgcgccgat cggaaagccg cagcccaagg cttttgccga actcaaaata tcgggaacaa
     3361 cgccgtaatg ttcataggca aacagcctgc ccgtatgccc catcccggtt tgaacttcgt
     3421 ccaaaatcaa caacgcattg tgccggtcgc acagacggcg cgcggtttgc aaatattccg
     3481 cagtggcggg caggatgccg ctttcgccct gtatcggctc gatgatgacc gcgcaggtct
     3541 gttcgccgac ggcagcttcc agcgcggcaa tatcgttgaa cggaacgtgc gtaatgcctt
     3601 gcggcagggg tgcataatcc ttgctgtatt tcggctgacc gccgacggac acggtaaaca
     3661 gcgtgcgtcc gtgaaaactg ttgatacagg cgacgatttc gctttttcct ccgccgaaac
     3721 ggtcgcgggc gtatttcctc gccagcttca acgccgcctc attcgcttcc gagcccgagt
     3781 tgcagaaaaa aaccttgtcg gcaaaactgt ttgcaaccaa tttttgcgcc aattcctgcg
     3841 ctggacgcgt cgtatagata ttggaaatgt gccacagctt gtgcatctgc gcgtttaaag
     3901 catcgacaag ggcagggtgg cagtgtccca gcgcattgac ggcgataccg cctgaaaaat
     3961 caatatattc acgccctttc gtatcccaaa cgcggctgcc tgaagcgcgt tcgggaatca
     4021 tcggggcaaa ggcgaaattg ggggtcagat agttttgcat aattgctcct gattgcggtt
     4081 ttgctcggga ttatgcaccg tttttcaaag cagataaagg gaaattgccg acaatatcgg
     4141 gcagcggtaa aaataaaccg aaatattttc aatcggatag atgaaaaatc aaaaaaattt
     4201 cgataaaatg cttgaggcgc ggcagcgttt tgatttagaa tctccccaat atttttatct
     4261 tcagacggca tttcatcatg caaggcaaaa aatttacaac cgcttattgc tattggtacc
     4321 acacagacgt gcggtacttt tttgcgcttg cttgaaatcc atcccatacg caaaacaggc
     4381 cgctcgaaaa cggggcggct tttttgttac cttcacacag gtcgcccgat tcggcaacac
     4441 acgacagaat caggaaaatg accatgaatg caatgcccca aaccgccgca caaaacgaaa
     4501 ccctgcacac ttgtagcatt attgtcgatg atgcacagga tttttccccc atgcttctcg
     4561 atgcagggcg ggacgacaca cttatcagcg aatcgatgat tccgcaaatc cgcaccgttg
     4621 ccgccctgat agccgccgaa cggcacgact tcagccgcag cagccccgcc gagtttaccg
     4681 atgccgccga tttttttgcc gcacggattc tcgtattggg cgtgcgacgc ttccatctgg
     4741 acgtatcgct gcttcagatt ttgaaaaccg ccaacagccg cgcccgccgc tttgccgaaa
     4801 aacaccgcct gtttttcaca cccgcacaag ccgaattgag cctgaacaaa cacaaaaacc
     4861 gccgcctgct gaccgtagaa accgaacacg aagtcgaaaa caaaggcaac ccagtcgcca
     4921 acagcctcgc atttgtccgc aaattgcacc ccctgcccct gtaaacatca cgatttgccg
     4981 gcagaatgct tgccaaaatt cccgacggcg atacaataaa accgattttc cacccaaaca
     5041 ggacacacat gaaaaaaatc atcgcctccg cgcttatcgc aacattcgca ctcgccgcct
     5101 gccaagacga cacgcaggcg cggctcgaac agcagcagaa acagattgaa gccctgcaac
     5161 agcagctcgc acagcaggca gacgatacgg tttaccaact gactcccgaa gcagtcaaag
     5221 acaccattcc tgccgaagca caggcaaacg gcaacaacgg gcaacccgtt accggtaaag
     5281 acgggcagca gtatatttac gaccaatcga caggaagctg gctgctgcaa agcctggtcg
     5341 gcgcggcggc aggcgcgttt atcggcaacg cgctggcaaa caaattcaca cgggcaggca
     5401 accaagacag tcccgtcgcc cggcgcgcgc gtgcagccta ccatcagtcc gcacgcccca
     5461 atgcgcgcac cagcagggat ttgaacacgc gcagcctccg tgcaaaacaa caggcggcgc
     5521 aggcgcagcg ttaccgcccg acaacgcgcc cgcccgccaa ttaccgccgc cccgctatgc
     5581 gcggtttcgg caggaggcgg taaacccggc gcaccaatgc cgtctgaaga gctttcagac
     5641 ggcatttttg catttgttag ggacattgtt atgttgccgt ttgattttca gacggcattt
     5701 tgtttccaag cgtttgatgt cgggatggca attctgattt acgtgcgttg gcagggcggc
     5761 attgtcatgc cgccgttggg ttttcagacg gcatcatatc gaacagtcaa gccgtagggc
     5821 gagcattctt gcccaccgtt gcaagggcgg gatttggtgg gcaggaatgc ccaccctgca
     5881 tctgaaaccc ttccgtgtca ttcccgtgca ggaaggaatc tagatatttg aatttacgga
     5941 aattttcaat cgttgccaaa gcggaaaagg g
//
LOCUS       DAWFBO010000123         5903 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFBO010000123 DAWFBO010000000
VERSION     DAWFBO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.123"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            124..2142
                     /gene="uvrB"
                     /locus_tag="WGO87_001605"
     CDS             124..2142
                     /gene="uvrB"
                     /locus_tag="WGO87_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0392946.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACDSIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPSNTIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDGGKGRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKENLLF"
     gene            2153..2944
                     /locus_tag="WGO87_001606"
     CDS             2153..2944
                     /locus_tag="WGO87_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392947.1"
                     /translation="MIKMKDIYESICCKSIRKIMYISDSFVAISGTGFIIKFQEKFYF
                     CCLYHCMSNEGKFEKNKLFGLSILYKTYLEIDPKTISESNVANYVLFPNPEQITKEFC
                     SSKIDDDLMLDEEFIPIEIIGENELIPCGYLDFDIEYWSNYLFSEKKPIENEILLVCG
                     FPFSDNEIDYTDDGEIKKVSHQVHIYAGKCKKDDIGFFVEKEDKNVSFDCNGVSGGIV
                     MRMIDGHSEWVGIAVRANNDIIRFIPYYLVAKSIIQNILNRNQQA"
     gene            3063..3134
                     /locus_tag="WGO87_001607"
                     /pseudo
     CDS             3063..3134
                     /locus_tag="WGO87_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3609..4325)
                     /gene="trmB"
                     /locus_tag="WGO87_001608"
     CDS             complement(3609..4325)
                     /gene="trmB"
                     /locus_tag="WGO87_001608"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0392948.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(4447..5850)
                     /locus_tag="WGO87_001609"
     CDS             complement(4447..5850)
                     /locus_tag="WGO87_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0392949.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLAAAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDIYRYGLGMTSDKEKALHYYRQAAEAGSLAAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
BASE COUNT         1430 a         1458 c         1497 g         1518 t
ORIGIN      
        1 ttgggtagcg attgggtttc agacggcata cattcaggat ttcaccgcct tatgccgtct
       61 gaaagacgtt tccgacccat ccctactaca cgctttgtcc gcaaacgcat cacaggcaag
      121 attatggaag tcattcgata ccccgattcc cccttcaaac tccaccaacc cttcccgccc
      181 gccggcgacc agcccaccgc cattgccggc ctgctcgaag ggctttcaga cggccttgcc
      241 tatcaaaccc tgctcggcgt aaccggttcg ggcaaaacct acaccatggc gaacgtcatc
      301 gcccaaagcg gccgacccgc catcatcatg gcgcacaaca aaacccttgc cgcccagctc
      361 tatgccgaaa tgcgcgagtt tttccccgaa aacgcggtgg aatatttcgt ctcctactac
      421 gactattacc agcccgaagc ctatgtgccc agccgcgact tgttcatcga aaaagacagc
      481 gcgatcaatg aacacatcga acaaatgcgc ctttccgcca ccaaaaacct gatgacgcgc
      541 gacgacgtga ttatcgtcgc caccgtgtcc gccatttacg gtatcggcga cccgactgag
      601 tatcaacaaa tggtgttgtc cgtcaaagaa ggcgacacca tcgagcagcg cgacatcatc
      661 gccacgctcg tttccatgca gtacgaacgc ggcgatttgg acttcaaacg cggcagcttc
      721 cgcgtgcgcg gcgacgtgat tgacgtgtac cccgccgaaa gctccgaaaa cgccttgcgt
      781 atcagcctgt tcgacgacga aatcgaccgc ctcgatatgt tcgacccgct ttcaggcagc
      841 cttatccaac gcgtcggccg ctacaccgtc ttcccgtcca gccactacgt taccccgcgc
      901 gacaccgtat tgcgcgcctg tgactccatc aaagaagaat tgcgcgaacg cattgatttc
      961 tttaccaaag aaaaccgtcc cgtcgaacaa caacgcatcg aacagcgcac ccgtttcgac
     1021 ctcgaaatgc tctacgaaat gggcttctgt aaaggaatcg aaaactactc ccgccacttc
     1081 tccggcaaaa aagaaggcga accgccgccc acgctgatgg actacctgcc aagcaacacc
     1141 atcatgttca tcgacgaaag ccacgttacc gttacccaaa tcggcggcat gtacaaaggc
     1201 gacgcctcgc gcaaacaaaa cctcgtagat tacggcttcc gcctgccttc cgcccgcgac
     1261 aaccgcccgc tcaaattcca cgaatttgaa aaagtcatgc cgcaaaccgt cttcgtttcc
     1321 gccactcccg ccaaatacga agaagaacac gccggacaag tggtcgaaca agtcgtccgt
     1381 cccacagggc tggtcgaccc ccaaatcatc atccgccccg tcgccaccca agttgatgac
     1441 ttaatgagcg aaatcaacga ccgtattcaa aaaggcgaac gtgtgctcgt taccaccctc
     1501 accaaacgca tggcggagca actcaccgac tattacagcg aactcggcat caaagtacgc
     1561 tacctgcaca gcgacatcga caccgtagag cgcgttgaaa tcattagaga tttgcggctc
     1621 ggcctgtttg acgtactcgt cggcatcaac ctcttgcgcg aaggcctcga catccccgaa
     1681 gtctccctcg tcgccatcct cgacgccgac aaagaaggct tcctgcgctc ccaccgcagc
     1741 ctgattcaaa ccataggccg cgccgcacgc aacgtgaacg gcgtcgccat cctgtacgcc
     1801 gataaaatca ccgactccat gaaagccgcc atcgacgaaa ccgaacgccg ccgcgaaaaa
     1861 cagattaaat tcaacgaaga acacggcatc gtaccgcagc agattaaaaa acaggtaaaa
     1921 gacattatcg acggcgtgta ccacgaagaa gacggcggaa aaggccgtct gaaaggtaaa
     1981 aacaaggtta aagtgggcga gattcacaac gaagaagatg cgattaaaga aatcgccaaa
     2041 ctcgaaaaag ccatgcagca ggcggctagg gatttgcagt ttgaagaggc ggctgtgttg
     2101 cgggatagga ttcgggggat taaggagaat ttattatttt aggatgtaaa aaatgattaa
     2161 gatgaaagat atttatgagt caatttgttg taaatctatt agaaagataa tgtatatatc
     2221 agatagtttt gtagctattt caggaacggg gtttattatt aaatttcagg aaaaatttta
     2281 tttttgctgt ttatatcatt gcatgtctaa tgaaggaaaa tttgaaaaaa ataaattgtt
     2341 tgggttaagt attttatata aaacatatct tgaaatagat cccaaaacta tatctgaatc
     2401 taatgtagct aattatgtac tttttcctaa tcctgagcaa attacaaaag agttttgtag
     2461 ttcaaaaatt gatgatgatt taatgcttga tgaggaattt atccctattg agattattgg
     2521 agaaaatgaa cttataccat gtgggtattt ggattttgat atcgaatatt ggagtaatta
     2581 tttgttttca gaaaaaaaac caatagaaaa tgaaattctt ttagtatgcg ggttcccttt
     2641 ttcagataat gaaatagatt atactgatga tggtgagatt aaaaaagtat cgcatcaagt
     2701 gcatatatat gctggtaaat gtaaaaaaga cgatataggc ttttttgttg aaaaagaaga
     2761 taaaaatgtt tcatttgatt gtaatggtgt tagtggtgga attgtgatga gaatgataga
     2821 tggacacagt gagtgggttg gaattgctgt tagggcaaat aatgacatca ttcgttttat
     2881 tccttattat ctcgtagcga aatcaataat ccagaatatc ttaaatagaa atcaacaagc
     2941 gtaggtcgga tacttgtatc cgacaaaacc ttcaacattc ccgtcgttgc aatccattgc
     3001 agctatgtcc gaaatgtcgg attcgaaaat ccgacctaca aaatcattcc gagcataaca
     3061 ctatgaaata acgtcgtttt taccgcaatg acggcactta cttttttacg gttgcaacca
     3121 ataaacggca gcaagcgtag ggtgggcact tgttgcccac gcgtttatct ccgaaaggca
     3181 gcaactgtag gtcggatact tgtatccaac aaacctttaa cattctcgtt gttgcaatcc
     3241 attgcagcaa tgcccaaaat gtcggattcg agaatccgac ctacaaaatc attccgaaca
     3301 taatactatg aaataccgtc gttttaccgc aatggcggca cttacttttt tacggttgta
     3361 accagcaagc cgtagcctac atcacaccaa ccgcgtgcgt agctatcaca tactacctgc
     3421 gggcggcaca aactttaaaa ccgatgtgca ggttaaaacc tacaaggatg cgtgcggtac
     3481 acacgcgttt tgctgttcgc accattgctt tgaactttat atcgaaacat cactcgctgc
     3541 tttatcttga aaaaatgccg tttgtaagca aacatgcttc agacggcatt tttttatccg
     3601 gcggtttgtt atccgatccg tttgaatacc aagtcccaaa cgccgtgtcc gaggcgtttg
     3661 ccgcgcgctt cgaatttggt ttcggggcgg tagtccgggg tgggggcgta gtctgccgcc
     3721 gtattttgca ggctgtcgaa gctgctgagg acttcaagca tctgctgtgc atattcttcc
     3781 cagtctgtcg ccaggtggat atagccgccg gttttgagtt tgggcagcag tttggcgatg
     3841 aagggggctt gtatcagacg gcgtttgtgg tggcgttttt tgtgccacgg gtcggggaag
     3901 aatatgtgga tgccgtcgag cgagccgtct tgcagcatat tttcgacaac ttctacggca
     3961 tcgtgccgca tcacgcggat attttccaaa tggttttcgt ctataagttt gagcagatta
     4021 cccacgccgg gaccgtgtac gtcgatggcg agaaagtcgg tttcgggcag cctgcgggcg
     4081 atttctgcgg ttgctgtccc cataccgaag cctatttcga ggattttggg ccggttgctt
     4141 ccgaaattgg catcaagatc ggccggtgtt gcttggtaat cgatgccgaa gctgtcccat
     4201 aaggtatcga tggcgcgttg ctgcgcggcg gtcatatggc tttggcggag gacgaagctg
     4261 cggatgctgc gggggtgtcc ggcttgacgg tcgtttggtg tttggttttc ggcggtgtct
     4321 gtcataatgc tttcatgcgc ttgtgcaatc aggatgccgt ctgaatgtga aatgtctgtt
     4381 cggacggcgg gaagggaaag gggcgattat acctgtttcg tttcggcagg gtaggtgtaa
     4441 cgctgttcag acggcatttt gccactgttg caacagttct tgcaggacgc ttttttgccc
     4501 gtatccgttg tttatggctt cttgcaggta gcggcaggct ttttctttgt ccggcccgac
     4561 accgtgtccg ctgtaatgga tgcaggcgag gttgtagaac gccatactgt ctttttttgc
     4621 ggcggctttt tcaaaccatt tgcgtgcttc attgtagtcg gcggtcatac cctgtccgaa
     4681 gtaatacatg ctgcccagat tggtttgggc tttgctgtgt ccgagttctg cggcttctgt
     4741 gtagagtttg agcgcggctg cgtattcggg cgcgcattgt aatccgtaat gcagggcttg
     4801 ggctttttga taaagccgtt ctgccctttg ccgcctgatg gcggaatctt taatgccgcc
     4861 atattggtcg ggatggttta acgcgctgtc ggatatgagt ttctgatacg cggcaaggct
     4921 gccggcttcg gctgcctgcc ggtaataatg cagggctttt tctttatcgg aagtcatgcc
     4981 cagtccgtag cggtagatgt cgcccagtat ccgcaaacct tcgggatggc gttcggcggc
     5041 ggctgcttcg gcgtgatgca gggcaagttt gtagtttctt tccggatgct tgccttgcag
     5101 gtagatgtcg gcaagtgcgg catgggcatc ggggtcattt tcctgtgccg cctgttgaaa
     5161 ccagtgaacg gcttcatcag gagccgtagg taggatgagc tcggcaagcg gggtgtaggc
     5221 gggaatgtat ccttgttgtg cggcggcgcg caaatgattg acggcctgtg ccgtgtcgac
     5281 tttcgtccct tgtccgaaac ggtattgcag cccgagctgc cagtgtgccg ccgccaagcc
     5341 ttgtgccgcc gctttgcggt agagcaggtg cgccgtttca aaatccggcg gcgtgccgta
     5401 ccggcagtat tgggcaagca ggtattgccc gtgggggttg ttgttttttg cgcttgtttt
     5461 tgcccatttc agggcttctt caaaatgtcg ggacgcatac agaaggtaca tcaggcggac
     5521 ttgtgcttca ggcagtcctt cctctgccaa cggtctgtaa agcggtatca gggtttccgg
     5581 gggcgcgcct tccaattctt ggatatatgc cagattgtaa cgcgcatagg gatgcccctg
     5641 ttcagcggcg atgcggagca tttccatacc ttgttcgcgg tcttggtagg ggctgtccgc
     5701 gtccatcagg tgttttgcca actcgaatgc ggcggcggga tgacctgccg agaacgcttc
     5761 tgtcagcagt cggcggactt cggtaaagtt atattgtttt tgagacaggc attctaaggc
     5821 ctgccgatag ggtaagtcgg gattgttcat gtcagatcgt tttccgtttt gtcgaatcat
     5881 cgttattgtg ttatgaagtt agg
//
LOCUS       DAWFBO010000124         5873 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFBO010000124 DAWFBO010000000
VERSION     DAWFBO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.124"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..1383
                     /gene="autA"
                     /locus_tag="WGO87_001610"
     CDS             <1..1383
                     /gene="autA"
                     /locus_tag="WGO87_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoaggregation autotransporter AutA"
                     /protein_id="HEZ0392950.1"
                     /translation="KLHVKIENKSHVAGQVLELTKMTLKDSLWEPRRHSDIHMLETSD
                     NARIRLNTKDEKLTVHKAYQGGADFLFGYDVRESDKPALTFEEKVSGQSGVVLERRPE
                     NLKTLDGRKLIAAEKADSNSFAFKQNYRQGLYELLLKQCEGGFCLGVQRLAIPEAEAV
                     LYAQQAYAANTLFGLRAADRGDDVYAADPSRQKLWLRFIGGRSHQNIRGGAAADGRRK
                     GVQIGGEVFVRQNEGSRLAIGVMGGRAGQHASVNGKGGAAGSYLHGYGGGVYAAWHQL
                     RDKQTGAYLDGWLQYQRFKHRINDENRAERYKTKGWTASVEGGYNALVAEGVVGKGNN
                     VRFYLQPQAQFTYLGVNGGFTDSEGTAVGLLGSGQWQSRAGIRAKTRFALRNGVNLQP
                     FAAFNVLHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGWKGHMSARIGYGKRTDGDK
                     EAALSLKWLF"
     gene            1625..3091
                     /locus_tag="WGO87_001611"
     CDS             1625..3091
                     /locus_tag="WGO87_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0392951.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQTDGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFDNRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(3292..4260)
                     /locus_tag="WGO87_001612"
     CDS             complement(3292..4260)
                     /locus_tag="WGO87_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392952.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            4338..5630
                     /locus_tag="WGO87_001613"
     CDS             4338..5630
                     /locus_tag="WGO87_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0392953.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
BASE COUNT         1309 a         1532 c         1820 g         1212 t
ORIGIN      
        1 aaattacacg tcaaaatcga aaacaaatcc cacgtcgccg gacaggtgtt ggaactgacc
       61 aagatgacgc tgaaagattc cctttgggaa ccgcgccgcc attccgacat ccatatgctg
      121 gaaacttccg ataatgcccg catccgcctg aacacgaaag atgaaaaact gaccgtccat
      181 aaagcgtatc agggcggtgc ggatttcctg ttcggctacg acgtgcggga gtcggacaaa
      241 cccgccctga cctttgaaga aaaagtcagc ggacaatccg gcgtggtttt ggaacgccgg
      301 ccggaaaatc tgaaaacgct cgacgggcgc aaactgattg cggcggaaaa ggcagactct
      361 aattcgtttg cgtttaaaca aaattaccgg cagggactgt acgaattatt gctcaagcaa
      421 tgcgaaggcg gattttgctt gggcgtgcag cgtttggcta tccccgaggc ggaagcggtt
      481 ttatatgccc aacaggctta tgcggcaaat actttgtttg ggctgcgtgc cgccgacagg
      541 ggcgacgacg tgtatgccgc cgatccgtcc cgtcaaaaat tgtggctgcg cttcatcggc
      601 ggtcggtcgc atcaaaatat acggggcggc gcggctgcgg acgggcggcg caaaggcgtg
      661 caaatcggcg gcgaggtgtt tgtacggcaa aatgaaggca gccgactggc aatcggcgtg
      721 atgggcggca gggccggcca gcacgcatca gtcaacggca aaggcggtgc ggcaggcagt
      781 tatttgcatg gttatggcgg gggtgtttat gctgcgtggc atcagttgcg cgataaacaa
      841 acgggtgcgt atttggacgg ctggttgcaa taccaacgtt tcaaacatcg catcaatgat
      901 gaaaaccgtg cggaacgcta caaaaccaaa ggttggacgg cttctgtcga aggcggctac
      961 aacgcgcttg tggcggaagg cgttgtcgga aaaggcaata atgtgcggtt ttacctgcaa
     1021 ccgcaggctc agtttaccta cttgggcgta aacggcggct ttaccgacag cgaggggacg
     1081 gcggtcggac tgctcggcag cggtcagtgg caaagccgcg ccggcattcg ggcaaaaacc
     1141 cgttttgctt tgcgtaatgg tgtcaatctt cagccttttg ccgcttttaa tgttttgcac
     1201 aggtcaaaat ctttcggcgt ggaaatggac ggcgaaaaac agacgctggc aggcaggacg
     1261 gcgctcgaag ggcggttcgg cattgaagcc ggttggaaag gccatatgtc cgcacgcatc
     1321 ggatacggca aaaggacgga cggcgacaaa gaagccgcat tgtcgctcaa atggctgttt
     1381 tgatgcgtcg ggaaatgttt tgacgcacag gcggcacacc ggcacggccc cgcgcgccgc
     1441 cccgcaaacc aatccgaacc ctgccgcccc gaagggcggg gcatcatgat gaaaccggcg
     1501 gaaaaccgcc ggttttttgc cgccgtttga aacccgattc tggcttcaga cggcattgtc
     1561 gcggcatcgg gcggcagggt ttggaacagc ggcataaaaa actgatacaa tccgccgatt
     1621 gataatggtt attttttatt tttgtgggaa gacatttatg cctgcacgaa acagatggat
     1681 gctgctgctg cctttattgg caagcgcggc atatgccgaa gaaacaccgc gcgaaccgga
     1741 tttgagaagc cgtcccgagt tcaggcttca tgaagcggag gtcaaaccaa tcgacaggga
     1801 gaaggtaccg gggcaggtgc gggaaaaagg aaaagttttg cagattgacg gcgaaaccct
     1861 gctgaaaaat cccgaattgc tgtcccgcgc gatgtattcc gcagtggtct caaacaatat
     1921 tgccggtatc cgcgttattt tgccgattta cctacaacag gcgcagcagg ataagatgtt
     1981 ggcactttat gcacaaggga ttttggcgca gacagacggt agggtgaaag aagcgatttc
     2041 ccattaccgg gaattgattg ccgcccaacc cgacgcgccc gccgtccgta tgcgtttggc
     2101 ggcggcattg tttgacaaca ggcagaacga ggcggcggca gaccagttcg accgcctgaa
     2161 ggcggaaaac ctgccgccgc agctgatgga gcaggtcgag ctgtaccgca aggcattgcg
     2221 cgaacgcgat gcgtggaagg taaatggcgg cttcagcgtc acccgcgaac acaatatcaa
     2281 ccaagccccg aaacggcagc agtacggcaa atggactttc ccgaaacagg tggacggcac
     2341 ggcggtcaat taccggctcg gcgcggagaa aaaatggtcg ctgaaaaacg gctggtacac
     2401 gacggcgggc ggcgacgtgt ccggcagggt ttatccgggg aataagaaat tcaacgatat
     2461 gacggcaggc gtttccggcg gcatcggttt tgccgaccgg cgcaaagatg ccgggctggc
     2521 agtgttccac gaacgccgca cctacggcaa cgacgcttat tcttacacca acggcgcacg
     2581 cctttatttc aaccgttggc aaaccccgaa atggcaaacg ttgtcttcgg cggagtgggg
     2641 gcgtttgaag aatacgcgcc gggcgcgttc cgacaatacc catttgcaaa tttccaattc
     2701 gctggtgttt taccggaatg cgcgccaata ttggatgggc ggtttggatt tttaccgcga
     2761 gcgcaacccc gccgaccggg gcgacaattt caaccgttac ggcctgcgct ttgcctgggg
     2821 gcaggaatgg ggcggcagcg gcctgtcttc gctgttgcgc ctcggcgcgg cgaaacggca
     2881 ttatgaaaaa cccggctttt tcagcggttt taaaggggaa aggcgcaggg ataaagaatt
     2941 gaacacatcc ttgagccttt ggcaccgggc attgcatttc aaaggcatca cgccgcgcct
     3001 gacgttgtcg caccgcgaaa cgcggagtaa cgatgtgttc aacgaatacg agaaaaatcg
     3061 ggcgtttgtc gagtttaata aaacgttctg attgctgttc cttttcggag gaaaccctgc
     3121 cggcggcggt atcacggcgg gcatcggcgg ctttcgggcg gtgctttgcg tgccgccgcg
     3181 tgtgcggaaa cgcattccgg tttttccggc ataacggcga tgcgaggtaa aatgccgtct
     3241 gaaacccgat tcgggcttca gacggcattg tcgcggttgc ggcgggcggg ttcaccagat
     3301 tccgtcaaag gttttcgcgc cgcgccaaaa tttccacctg tcggcgggtt tgaaggtcag
     3361 cgtaccgccg tgttgtccgt ccgtggtgat gtccagccgt ttgattttgc cggtacggac
     3421 ggcttcgtag atcggtgcga accagtgttc ttcccactgc tgtaaaaccg atgcataccg
     3481 ctccctgtcc cctgtcaggg cggtcaggcg caaatcgtcc ataaacagga tatggtgcgt
     3541 gtcgggcagg tgtgccgccg tttcttcata ggcgcggaag ttgtcgggca atgcgcggcg
     3601 gtcggggtgg aaacggctcc aaaccgtatc ggcgaaaagc gtgccgcctt gactgcttcc
     3661 gtgcagactg tcccaaagcc ataagccgtt caactcgggc agcccgcgtt tcttgcggtt
     3721 atggttgacg gggtgcgccg ccagccacat ttggatttcg gtttggacgc gcagccattc
     3781 caacgcgtct tccccgtccg gttgatcgtc cgcacccaac agtccgccca agtccaaaac
     3841 gggcttcgcg ccccagcggt acgcgcaagg aagggaaacc agccataatt cgggcaggac
     3901 gggaacgaaa cgccatggaa tgtcgccgta aaacgccgac aggtcgcggc agagccgttc
     3961 cgcttcatcc gtaccgacgt tcagatattc cgccgttagc acatttgcct gatgcatccc
     4021 catcttttgc cagacgggcg tggcgagcgc gacggcttca gacggcatat tcaggctttg
     4081 tgccgcgcgt tccgccagtc tgccgcacca caaataacgc gcgtaaaatg ccgaagccgt
     4141 gcagctttgg cggtgcagcg agccgtattg caggattttg ttgaaggcgg gcaggcagag
     4201 aggtattcgg gtttcgtctt catccaaatt gagcgaggga atggcgaggg tgagtttcat
     4261 cgtttgacgt ttcagaaatg caggtcaggc gcaacattat agaggattcg gcgcaaacgc
     4321 cgtcaaaaag gaacaatatg gctgtcttcc cactttcggc aaaacatcgg aaatacgcgc
     4381 tgcgcgcgct tgccgtttcg attattttgg tgtcggcggc atacattgct tcgacagaga
     4441 ggacggagcg cgtcagaccg cagcgcgtgg aacaaaaact gccgccgctg tcttggggcg
     4501 gcagcggtgt tcagacggca tattgggtgc aggaggcggt gcagccaggc gactcgctgg
     4561 cggacgtgct ggcgcgttcg ggtatggcgc gggacgaaat tgcccgaata acggaaaaat
     4621 atggcggcga agccgatttg cggcatttgc gtgccgacca gtcggttcat gttttggtcg
     4681 gcggcgacgg cggcgcgcgc gaagtgcagt tttttaccga cgaagacggc gagcgcaatc
     4741 tggtcgcttt ggaaaaaaaa ggcggcatat ggcggcggtc ggcttctgag gcggatatga
     4801 aggttttgcc gacgctgcgt tcggtcgtgg tcaaaacgtc ggcgcgcggt tcgctggcgc
     4861 gggcggaagt gcccgtcgaa atccgcgaat ccttaagcgg gattttcgcc ggccgcttca
     4921 gccttgacgg tttgaaggaa ggcgatgccg tgcgcctgat ttacgacagc ctgtatttcc
     4981 acgggcagca ggtggcggcg ggcgatattt tggcggctga agtcgttaag ggcggcacaa
     5041 ggcatcaggc gttctattac cgttcggaca aggaaggcgg agggggcggc aattattacg
     5101 atgaagacgg cagggtgttg caggaaaaag gcggcttcaa catcgagccg ctggtctata
     5161 cgcgcatttc ttcgccgttc ggctaccgca tgcaccccat cctgcacact tggcggctgc
     5221 acacgggcat cgattatgcc gcaccgcagg gaacgccggt cagggcttcc gccgacggcg
     5281 tgattacctt taaaggccgg aagggcggat acggcaacgc ggtgatgata cgccacgcca
     5341 acggtgtgga aacgctgtac gcgcacttga gcgcgttttc gcaggcacaa ggcaatgtgc
     5401 gcggcggcga ggtcatcggt tttgtcggtt cgacagggcg ttcgaccggg ccgcacctgc
     5461 attacgaggc acgcatcaac gggcagcccg tcaatcctgt ttcagtcgca ttgccgacac
     5521 ccgaattgac gcaggcggac aaggcggcgt ttgccgcgca gaaacagaag gcggacgcgc
     5581 tgcttgcgcg cttgcgcggc ataccggtta ccgtgtcgca atcggattga agtttgaacc
     5641 ggcgacgaaa acaatgccgt ctgaaaatct gcaaacaggt tttcagacgg catttatagt
     5701 ggattaacaa aaaccagtac gtcgttgcct cgccttagct caaagagaac gattctctaa
     5761 gatgctgaag caccaagtga atcggtttcg tactatttgt actgtctgcg gcttcgtcgc
     5821 tttgtcctga tttttgttaa tccaccatac agtcgattaa aataaaacta agt
//
LOCUS       DAWFBO010000125         5836 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFBO010000125 DAWFBO010000000
VERSION     DAWFBO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.125"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            13..189
                     /locus_tag="WGO87_001614"
     CDS             13..189
                     /locus_tag="WGO87_001614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392954.1"
                     /translation="MAHQAPHCVLPERDYPPIRFYRYKQTDFNRKGMGIKSVSDTSRA
                     IPSENQSPLSDGIV"
     gene            266..1213
                     /locus_tag="WGO87_001615"
     CDS             266..1213
                     /locus_tag="WGO87_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0392955.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAIAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFEHPKVVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAG
                     SMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1366..2490)
                     /gene="rluD"
                     /locus_tag="WGO87_001616"
     CDS             complement(1366..2490)
                     /gene="rluD"
                     /locus_tag="WGO87_001616"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0392956.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIIYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2489..3292
                     /locus_tag="WGO87_001617"
     CDS             2489..3292
                     /locus_tag="WGO87_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0392957.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3350..5569)
                     /locus_tag="WGO87_001618"
     CDS             complement(3350..5569)
                     /locus_tag="WGO87_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0392958.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWILTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPSVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1421 a         1833 c         1406 g         1176 t
ORIGIN      
        1 tttcaacagg ggatggcaca tcaagcaccg cactgcgtgt tgcccgaacg agactaccct
       61 ccgattagat tctatcgcta taaacagacg gatttcaacc ggaaaggaat ggggataaag
      121 tccgtttccg acacctctcg ggcgataccg tctgaaaacc aatctccact ttcagacggc
      181 attgtttagg cacggtcatt ttgcaaatta aggttaaggc gtgtatgctt gccttccctt
      241 aaaaacaaac acgcaacgca ttattatgaa tatcctcagt aaaatcagca gctttatcgg
      301 aaaaacattt tccctctggg ccgcgctctt tgccgccatc gcttttttcg cgcccgacac
      361 cttcaaatgg gcggggcctt atattccttg gctgttgggc attattatgt tcggtatggg
      421 tttgacgctc aaaccttccg acttcgatat tttgttcgaa catcccaaag tcgtcatcat
      481 cggcgtaatc gcacaattcg ccattatgcc ggcaaccgcc tggctgctgt ccaaactgtt
      541 gaacctgcct gccgaaatcg cggtcggcgt gattttggtc ggctgctgcc cgggcggtac
      601 ggcttccaat gtgatgacct atctggcgcg cggcaatgtg gctttgtcgg ttgccgttac
      661 gtctgtttcc accctgattt ccccattgct gactcccgcc atcttcctga tgcttgccgg
      721 tgaaatgctg gaaatccaag cgggcagtat gttgatgtcc atcgtcaaaa tggttttgct
      781 ccccattgtt ttgggtttga ttgtccataa ggttttgggc agtaaaaccg aaaagctgac
      841 cgatgcgctg ccgctggttt ccgttgccgc catcgtgctg attatcggcg cggttgttgg
      901 ggcaagcaaa ggcaagatta tggaaagcgg cctgctgatt tttgcggttg tcgtactcca
      961 caacggcatc ggctacctgc tcggcttctt tgccgccaaa tggaccggcc tgccttatga
     1021 tgcacaaaaa acgctgacca tcgaagtcgg tatgcaaaac tcgggcctgg ccgccgcgct
     1081 tgccgccgca cactttgccg ccgcgccggt cgttgccgtt ccgggcgcat tgttcagcgt
     1141 gtggcacaat atctccggct cgctgctggc aacttattgg gcggccaaag ccggtaaaca
     1201 taaaaaaccc taaacgcctt tcatcataat gccgtctgaa cggggcaaac aaaactggcg
     1261 gtttctgccc ggccccttca gacggccttt gtttgaaatc ggcggggacg attgcccggc
     1321 tgctccgatt cggctgcctg cgttcgcccc gcctttcaag ccgcctcact cccttacata
     1381 aaccacttcg acatcgtagt cgtcttcgtt ccaatcgtca tcgtcgtccg cgccgaattt
     1441 gtcctgccat tcctcttcat tgctcaaaga cgaatccaaa ccggcttcaa gacggaggac
     1501 agacaacaaa tgatatatgt cgtccggaat cggtgcttca aacgaaacgg tttcgccggt
     1561 tttcggatga gtgaaactta agcggtaggc gtgcaatgcc tgacgcgcac ccaaactttt
     1621 aacggcttct ttcaccgtgt cgccgcacgg atggcgcggg ttgccgtaaa ccgggtcggc
     1681 ggcaagcgga tggttggcct cacgcatatg gacgcggatt tggtgcgtcc tgcctgtttc
     1741 gagcgagcat tcgatatagc tgtgcgccag atagcgttcc aacactttga cgtgggtaac
     1801 ggctggtttg ccgccgaatt tgacgactgc cattttcagg cggttgtgcg gatcgcgtcc
     1861 gatttgggtt tcgattttac cgtcaaaggg aacgatgccg ttggcgacgg cgcggtaaat
     1921 gcgtttgacc gtgcgctctt gaagctgttg cacgagggaa ttttgcgccg gcagggtttt
     1981 ggcaaccacc atcagcccgc tggtttcttt gtccaaacgg tgtacgatgc ctgcacgagg
     2041 tacttggctc aattcgggac agtgcgccaa caggccgttg agcagcgtcc ccgtccagtt
     2101 gcccgccgcc ggatgcacca ccagtccggc cggtttgttg acgacgatga cggtatcgtc
     2161 ttcgtaaata atatccaaag ccataggctc tggaacaaac gccagatttt cctcactcgg
     2221 acggacggtt acacaaattt gctcgccgcc tatcattttg tctttgggtt gcgaaggttt
     2281 atcgtttaca ataaccgcgc cttctttaat ccatgatgtc aggcggctgc gcgagtagtc
     2341 gggcagaagt ttcgccaaca ccgcatccaa ccgcccgccg gcaagctcga gcggaacggt
     2401 cagaccgata cggttttcag tttcggaagc tgacgcaaag tctgaatcgt cgctataatc
     2461 ggattcatta tcaaaggaag tattctgcat gaaaaaaatt cttttaacgg tttcattagg
     2521 tttggcactg agtgcctgtg ccactcaagg tacggtcgat aaagatgctc agattaccca
     2581 agattggagt gtggagaagc tctatgccga agcccaggac gaattgaaca gcagcaatta
     2641 tacgcgggct gtcaagttat acgaaatctt ggaatcgcgc ttccccacca gccgccatgc
     2701 ccggcaatcc caactggata ccgcatacgc ctattataaa gacgatgaaa aagacaaggc
     2761 tctggcggca atcgaacgct tccgccgcct ccatccgcag catccgaata tggattacgc
     2821 gctgtatctg cgcggcttgg tgctgttcaa cgaagaccag tccttcttga acaaactggc
     2881 ctcgcaagac tggtccgacc gcgacccgaa agccaaccgc gaagcgtatc aggcctttgc
     2941 ggaactcgtc caacgcttcc cgaacagcaa atacgccgcc gatgcaaccg cacgcatggt
     3001 caaactggtc gatgcactgg gcggcaatga aatgtcggtg gcgcgttact acatgaaacg
     3061 cggcgcatat atcgccgccg ccaaccgcgc ccaaaaaatt atcggcagct atcaaaatac
     3121 acgctatgtc gaagaatcac tcgccatatt ggaacttgcc taccaaaaac tcggcaaacc
     3181 gcagcttgcc gccgatacgc gccgcgtgtt ggaaaccaac ttcccgaaaa gcccgttttt
     3241 gacgcacgcc tggcagcccg acgatatgcc ctggtggcgt tactggcatt aagcccatct
     3301 tcgtaatgcc gtctgaagcc gtttcagacg gcattttatg tttcaaacct cattcaaacg
     3361 gttttttctg ccaatagaat ttataccctc tcaaacgttg agccttcacg ccgccgcgtc
     3421 ccaagccgaa ttgcagtgcg cccgacaaat cggtacgcag cagtttgatg ccgtgtgcgc
     3481 ggacacggtt ctgcaccgct tcggtcggat gtttgtaggc gttggcataa ccgcttgaag
     3541 caacggcata ttcgggcgag acggcattga ggaacacgcc cgacgaggac gtattgctgc
     3601 cgtgatgtcc caaaaccaaa acctggctgt acaggttgcc tccatacttg ccgaccaggc
     3661 tttcctcgcc cttcgtatcc aaatcgcccg ttaccagcag tgccgcaccg cccgccacaa
     3721 cacgcaaaac acaacttttc ccattatcat cgatgttttt gcgttcagac ggcatcaaaa
     3781 actcaaaatc cacgccgtcc cattgccaac gctgttccgc acaatgccgc gccccctcat
     3841 aaaattccgg ctgcccggca taaatcccgc cgttcggtat cttgccgact gcctgaaaac
     3901 cgccgtcgtg gtcgctgtcg tgatgcgaca gaaccagctt gtccaaacgg cggacacccg
     3961 ccgcattcaa actcggcaca atccccatct gcgccgccgc tgccgttccg gtgtcaaaca
     4021 aaagatgatg atttgccgtc tgaaccgaca ccgacaaacc ctgccccgca tcccaaaccg
     4081 taaccgcaac ctcattttcc ggcacgctgg gtgaacggta aaacacaaac cctgccaaca
     4141 gcaacaccgc ccacggacgc aaacccaagc cgcgcggcag cagcaacagc aaagcggcac
     4201 acaccgccaa caccaacagc ggcaaaggtg cggaggcaac ggcaaactcg ggcgacacat
     4261 cggcaagcca caccaaaaac cgcaaagtat attccgccaa aaatgccccc aactgttgca
     4321 aaggcgcaaa cggcacgacc gaacccagca acgccagcgg cgtcaatatc caagaaaacc
     4381 acggaattgc caccgcattg accaaagggc tgattaaagg cagcgaagca aacagataac
     4441 cgagcaaaac cagcgacaac accgaagccg cccactgccc gcgcaaagca gtttgccgtt
     4501 ttccctcgtg caaacgcccc gagcacgccc atatcagggc cgccaccaaa ccgaaagaca
     4561 gccaagtccc cacacccaag accgccgacg ggtcgaacag cagcaccgcc gccaacgcct
     4621 gccaccacgt cgcccacgcc gacaatcttc ccctgcgcca agcccacgca aacgccgcca
     4681 acatcaaaac gctgcgctgc gtcggcacgg aaaaaccggc aagcagcgcg taaaacagcg
     4741 cgcctgcaca ccccgccgcc aaaacccacg cgcgcggccg ggcaggaagg cgcggggaac
     4801 aggcaagcag ccgcttcgcc agccacgcaa acattaccgc caccatcgta acgtgcaaac
     4861 ccgaaatact gaccaaatgc gtcagcccca acggccggaa cgcctgccac aattcggggc
     4921 gcaatgccga ctgttcgccc acgctcaacg cgcgcatcag cccgatgccg tctgaaagcc
     4981 cgccgtccgc atccgcctgc tgccaattac ggctgatgcg gctgcgccaa accgcaatcc
     5041 cccaaccgct tccgccatgc agcaaaaccc tgtccgcacc gaccgtcccc gcgccgccta
     5101 ttccattgga taatgcccac gcctcacggt tcaaaccgcg caggttcaac tccccgacga
     5161 cagggtgcac acgtgccgtt atccgccatc tgctcccgac cgcccattcg cgccgtttgt
     5221 agtccgacag cagcaaatca aacgtccgac caccgctgtc cacagccttt gccgcaaact
     5281 gcacacgccg cccgtccgac ctcggcatat ccgccacttc caccgtcaaa ggcacgcctg
     5341 aaacggcttc tgcccaccat tgggaagaca atgccgcctc cgtcctgaat acgccgtaag
     5401 ccgcgcccgc caacacgcac agcatcagac cggcaaacgc aaaacgcctt gcaaacacag
     5461 caaaaaccgc aaaagccgcc agccaaaaca gccaatgcgg cacgaccggc agcgcgaacg
     5521 aagccgccgc accgacaacc caacacggca aaaaccaacg acggaacacg tctccccacc
     5581 ccagatattt caaaaataaa cggataataa agcaaatccg aaaaaccgtc ataacaagat
     5641 ttgaaaatga tatagtggtt taaatttaaa tcaggacaag gcgacgaagc cgcagacagt
     5701 acaagcagta cgacaaggcg aggcaacgct gtactggttt ttgttaaacc actataagaa
     5761 tgcaaacgaa aagcctcagt attatccgag acaacggcaa actttccatc gtcattccca
     5821 cgaacccaca tcccgt
//
LOCUS       DAWFBO010000126         5807 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFBO010000126 DAWFBO010000000
VERSION     DAWFBO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.126"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            131..565
                     /locus_tag="WGO87_001619"
     CDS             131..565
                     /locus_tag="WGO87_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0392959.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1441)
                     /locus_tag="WGO87_001620"
     CDS             complement(578..1441)
                     /locus_tag="WGO87_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0392960.1"
                     /translation="MELNEFFDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1449..2066)
                     /locus_tag="WGO87_001621"
     CDS             complement(1449..2066)
                     /locus_tag="WGO87_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0392961.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2229..2843)
                     /locus_tag="WGO87_001622"
     CDS             complement(2229..2843)
                     /locus_tag="WGO87_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ0392962.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQIDLQEG"
     gene            3049..4821
                     /locus_tag="WGO87_001623"
     CDS             3049..4821
                     /locus_tag="WGO87_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0392963.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAVENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            4818..5474
                     /locus_tag="WGO87_001624"
     CDS             4818..5474
                     /locus_tag="WGO87_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0392964.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1441 a         1634 c         1339 g         1393 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gccagctttc ccacgccgcc acggcttata tccccctgaa
      241 cgattttcaa ccgaactgcg acatacgccg gctcggactg acacagggtc agcacaatga
      301 actgcgtaaa atccgcgccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gccggtctgt cgtcgaaatc atttcttcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accgcttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca caacgcccgt tgtttcggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggattt
      841 ccccccgcat ccccgtcgta ccggcattct cgtcaaaata ttcgggcatc aggtgccgtc
      901 tgttggaagt cgcgtaaacc aaaacgttgg cgcaacgttg agacagaccg ccgtctaacg
      961 cggttttcaa tgccttatag gtttcatcgc cgctttcaaa cgacaaatcg tcgcaaaata
     1021 cgataaattt ttccggacgt tccttcaaaa gcgtcaacag gtaaggcagg ccgattaaat
     1081 cgcttttatc gacttcgatc aggcgcaatc ccttatccgc atattcgtgt agcagggctt
     1141 tgaccagcga ggatttgcct gttccgcgcg cgccgctcat caatacattg ttcgcgggtc
     1201 tgccgacaat gaactgttcg gtattacgca ccagcaattc ggtttgcctg ccgactcccg
     1261 ccagccttac caagggaaag gtgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc
     1321 ccgcactctg ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa
     1381 gcacggcatc caaacgccgc aaaacggcat aggctttatc gaaaaattcg ttcaattcca
     1441 tctctacctc actttgcata tctttgcgcc atcagccgtt cgacggtgtc gacgattgcc
     1501 tgcgtattcg gatcgatttc gatgttgatc ctgtcgccga cctttctgct gccgaacagc
     1561 gtccgttcca aagtttcggg aatcagatgg acattgaaac ggccgtcttc gactttgcct
     1621 atggtcaggc tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt
     1681 tcatgcggga gcgaaaacca aaccgtgcgg ttgaacccgt cccgttcgat ttcgacaata
     1741 ggcacggttg ccataatgtg tccgctcatg acgtgtccgc cgatttcgtc tccgaaacgc
     1801 gccgcccgtt cgatgttgac gcaatcgcct tccttcagca gccccaaatt ggtttttgcc
     1861 aaagtttccg ccattaaatc gaaactgacg cggtttcctt cgatttcggt aatcgtcagg
     1921 cagcagccgt tattggcgac cgatgcgccg cgttgcagat tgtccgccgc ctcttgcgga
     1981 agctcgacga cataagtgtg aaatgcctcc gacgggcggt ggattgccgt cagttttccc
     2041 aatccttgaa caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca
     2101 aattgtagca tctccccgcg aaaaatgccg tctgaaatgc cttcagacag cattatgcct
     2161 ccgattcggg caaaaaccgc ccggtatggc ttgacctttc ctttccacgc cggtcggcgg
     2221 tcttgccctt atccctcctg caaatcgatt tgcgtgttca agtcggcaaa atgcccgtca
     2281 aactcgaatc tgaccgaccg cgccctttgc tgctgcaacc agcttcttaa tgttttcata
     2341 cccgaaaaca gatagggaat cgcgctttgc agaatctgcg ggcggatata cataatgttg
     2401 tagtgcatcg ttatcggcgt ttccacataa tacgcattgc acaacggtgt gcgtttcgac
     2461 acggtttcaa acctcgccac caaatcgtcc ggcagatacg gcatatcgca cggcacaacc
     2521 aaaagccagt cagcagtcgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc
     2581 gggccgaaat gctgccactg ccgcgcatcg ggaaaaatat gcggacttct tcgagcatat
     2641 tcttccaaat tccggttggt gctgatggcg atatggctga cctgcggcct gaccctgtcg
     2701 atgacatggt ctatcagtgc cttacccccc aaaagagcaa gccccttgtc ctcgcctccc
     2761 atacggctcg cctgaccgtc ggccagtatc agggcaaaag ttttcattgc ggatgttctc
     2821 ttggaaaagt tcgaggtttt catgattgca gtctgccgtt cccaatgact caaaatgccg
     2881 tctgaagcag atggcaaata aattcatatt atctgaattt tatcataaca tgatttaaca
     2941 ctgaagcggt gcggattaag ttttccttaa ccaattcttt ctgagcagtt gtatctaatc
     3001 ccaaagaatg atattgtttg cattatttag aacaattttt cgccgagcat gatactgcca
     3061 gcccgttttt cagacggcat cagcctttcc ctacgcctga aactcctgac cggagtgtgg
     3121 gtcgggttgg cggcattgtc tgtcgttttg acactgctgc tctctttgcg tctggaaaac
     3181 gcggcctccg tcatcgaaga ggcgggcaac ttgagaatgc aggcataccg tctggcatac
     3241 atggcgggtg aaggatctcc ccgctcccaa atagacaatc aggttgacga atttgaaaaa
     3301 agcctgaatc gtatttcaca aagtgatgcc atccatccgc tgattccttc ggacacccct
     3361 cttgcttatg atttgataca atccatgctg attatagatt ggcaggcaca catcctcccc
     3421 ccgctccagt cctaccggcg accgactcag gtcgatctct accgctttgc cggaaacatc
     3481 gaactgtttt tgcaggcatt ggaaaatgcc aacgaaaaaa acacatggtg gctcaggcgt
     3541 tttcaatggg caattatgtt gatgacgctg gtgtcgtctg tactgatgct gttttggcac
     3601 cagatttggg ttatccggcc gctgcaggcg ttaagggaag gtgcggaacg catcggacgg
     3661 aggtgtttcg atattccggt tcccgaaggc ggtacgccgg aattcaaaca ggtcgggcgt
     3721 tgtttcaatc aaatgggcgg caggttgaaa attttatatg atgatttgga aggacaagtc
     3781 gccgagcaga cacgcagtct cgaaaaacaa aatcaaaacc tgaccctgct gtaccaaact
     3841 acacgggacc tgcaccaatc ctacataccg caacaggctg cagaacattt tctaaaccgt
     3901 atcctgcccg ccgtaggagc agattccggc agagtttgtt tggacggcgg atccgatgtt
     3961 tatgtttcca ttcatcatgc ggattgcggc acagcagctt cggatttggg gaagtaccat
     4021 gaggaaatct tccccattga gtaccagaac gaaacattgg gcaggctgtt gctcagcttt
     4081 ccaaacggca tttctcttga tgaagacgac cgcatcctgc ttcaaacact aggcaggcaa
     4141 ttgggcgtat cgcttgccgg cgcaaaacag gaggaagaaa aacgcctgct tgcagtattg
     4201 caggaacgca acctgattgc gcaaggatta catgacagca tcgcacaagc attaacgttc
     4261 ctaaacctac aggtacagat gctggaaacc gcctttgccg aaaacaaacg ggaggaagcc
     4321 gtagaaaaca tcggcttcat caaaacaggc gtgcaggaat gttatgaaga tgtccgcgaa
     4381 ctgctgctca acttccgtac caaaatcagc aataaagaat ttcccgaagc cgttgccgac
     4441 ctattcgccc gctttacgca acaaaccggg ataacggtcg aaaccgcctg ggaaaacggt
     4501 tcgttcctgc cgcctcagga agcgcagctc caaatgattt ttatcctgca ggaaagcctg
     4561 tccaacatcc gcaaacacgc ccgcgccacc catgtaaaat tcaccctttc cgaacacggc
     4621 ggacgcttta ccatgaccat ccaagacaac ggacaaggtt tcgacacgga gaaaatagga
     4681 gaacccacgg gcagccatgt cggactgcac atcatgcagg agcgtgccaa acgcatccat
     4741 gccgttttag aaatccgttc ccaagctcaa cagggaacca ccgtctcatt gacggttgca
     4801 tctgaagaaa gcttgaaatg accattaaaa ttatcctgat agacgaccat accctcttcc
     4861 gcagcggcat taaatccctt ttgtcgcgcc aacatgattt tgaggtcatc ggcgaagccg
     4921 cagacggcct ctcgggcatt aaaatgatca accggctcca acccgatgtc gtcctgcttg
     4981 accttgatat gcccggtatg aacggacgcg aagcactctc ccaaatcatc agcatcaatc
     5041 cgcagcaggc agtgattatg ctgaccgttt ccgaagacaa cgacgacttg accgaatgta
     5101 tgcgcatcgg cgcgcgcggc tacctgctga aaaacatcaa cgccgacttt ctgctcgaaa
     5161 gcatacgcaa agccgccgaa ggcgataatg tattctcgcc cgagatgacc gccaaactcg
     5221 tcaaaagcct gatttccccc caacctgccc aagggacgca ggcactctcc tcacttaccc
     5281 ctcgtgaact ggaaatcttg ggctatctcg ccgcaggaca cagcaacaaa atcatcgccc
     5341 gccacctcga tcttgccgaa tccaccgtca aagtccacgt tcaaaacctg ctccgcaaac
     5401 tcaacctcag cagccgggtg caggccgctg tttacgccat ccggcacaac gtcccccaac
     5461 ccgtgctgga ataaggcggg ttcagacggc atattagggg ttttaatccc cgtacggtca
     5521 ttcggataac agaccaagca tgtaagttta tgcccccata agtacgcttg gcatagcagt
     5581 aatattgttc ggttcagtgt tttccgtttg cccctatctg atactgcaat atcagctatg
     5641 ccgtctgaaa acgcatcatc atgatatttt cagacggcat aataaaaagc ggaaatacta
     5701 atgcagggta aaatgttcca ttccgaatcc cataaatata caatggctta tatcgtttaa
     5761 gcatgtgtaa cccaccctca tatcaatcaa tatatagtgg attaaca
//
LOCUS       DAWFBO010000127         5735 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFBO010000127 DAWFBO010000000
VERSION     DAWFBO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.127"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(83..1129)
                     /gene="recA"
                     /locus_tag="WGO87_001625"
     CDS             complement(83..1129)
                     /gene="recA"
                     /locus_tag="WGO87_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0392965.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1383..2147
                     /locus_tag="WGO87_001626"
     CDS             1383..2147
                     /locus_tag="WGO87_001626"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0392966.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2167..4182
                     /gene="rep"
                     /locus_tag="WGO87_001627"
     CDS             2167..4182
                     /gene="rep"
                     /locus_tag="WGO87_001627"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0392967.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4250..5308)
                     /gene="dinB"
                     /locus_tag="WGO87_001628"
     CDS             complement(4250..5308)
                     /gene="dinB"
                     /locus_tag="WGO87_001628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0392968.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLIARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT         1411 a         1507 c         1536 g         1281 t
ORIGIN      
        1 aaactcaaat caagccgttc ggaggctgct caaaaaaaac ggtacttcgc aacagaagta
       61 ccgtttatcg ggatttcagg ttttattctt cggggcgttc gccgtcggtt tcgtcctgcg
      121 tcccttcggt gatgtgcatt tctatgccgt tgagggcgcg gatttttgcg tcgatttcgt
      181 cggcaatttc gggattttcc ttcagccaga cgcggacgtt gtctttgccc tgaccgattt
      241 tcgcgccgtt gtagctgtac cacgcgccgg atttgttgat gatgtcgttt ttcacgccga
      301 tgtcgatcaa ttcgccttcc caactgatgc cttctccgta gaggatgtca aactctgcct
      361 gacggaacgg cggcgcgact ttgtttttga tgactttgac gcgggtttcg ttgcccaata
      421 cttcttcacc ttttttgatg gatccggtgc ggcggatgtc gaggcggacg gaagaataga
      481 atttcagcgc gttgccgccg gtcgtggtct cggggctgcc gaacattacg ccgatcttca
      541 tccggatttg gttgatgaac acaaccagcg tgttggtttt tttgatgtgt ccggtcagtt
      601 tgcgcaaagc ctggctcatc aggcgcgcct gcagtccgac atggctgtcc cccatatcgc
      661 cttcgatttc ggctttgggg acgagtgcgg ctacggaatc gacgactacc atatctatgc
      721 cgcccgaacg gacgagtgtg tcgcagattt ccaaagcctg ttcgccggta tcgggctggg
      781 acaggtaaag ctcttcgact tttacgccga gtttgcgggc gtaaacggga tcgaaggcgt
      841 gttcggcatc gacaaaggcg cacacgccgc cgtttttctg gcattgggcg acggcttcga
      901 ggcagagggt ggttttgccg gaggattctg ggccgaagat ttcgacgatg cgcccgcgcg
      961 gcagaccgcc gactccgagg gcgaggtcta atccgagcga tccggtggaa atgacttcga
     1021 ggttttcttc ctgctggctg ccgtccattt tcatgatggc gcctttgccg aaactttttt
     1081 cgatttgcgc cagtgcggcg gcaagtgctt tgcttttgtc gtctgacatt ggggttactc
     1141 cggaacaaat gcggtatgtg ggatgcggcg caacacgggc tgcggcgcgg gatgtgtatc
     1201 gttttcccga tgtgcgggct atcggtaatg ctgcttcacg aggttgccat tatcgcatat
     1261 ttccttgctt gccgatatgc gccaggacgc ggcggcttgt gccggaatgg aatctggatg
     1321 ccgtctaaaa ggcggccggc tttgttataa tggcggctgt tttttctgtg tgtgcctgtt
     1381 ttatgtgttc ctgccttgtt gtcaaaaata ccgttatcgg aagcggacgc accaaaatcg
     1441 ccgtgccgct tgtcgcccgc gatgccgccg aactttccgc cgtacttgag caaatcaaaa
     1501 atatgccctt cgatattgcg gagttccgcg ccgacttttt ggaatgcgcg ggcagtatcg
     1561 gcgaaatatt gcaccacacg cagaccgtcc gcgacgcgct gcccgacaag ccgctgctgt
     1621 ttacgttcag acggcatggc gaaggcggct cgttcccgtg ttcggacgat tattatttta
     1681 aactgctcga cgcgctgatc gaaagtcgcc tgcccgacat tatcgatatc gagctgtttt
     1741 ccggcgaaac cgccgtccgg cgcgctgtgg cgaatgctca aaaaaacggc atcgccgccc
     1801 tgctctgcaa tcatgagttt caccgcacgc cgccgcaaga agaaatcgta tgccgtctga
     1861 aacagatgga ggactgcggc gcggacatct gcaaaattgc ggtgatgccg caaagcgcgg
     1921 aagatgtgct gactttgctt tccgccacgc tcaaagcgaa agagcttgcc gccaaaccga
     1981 ttgttacgat gtcgatgggg cagacggggg cggtcagccg gcttgccgga caggtgttcg
     2041 gctcaagcat cacgttcggt tcgggaacgc aaaactccgc gccggggcaa atcggcgtat
     2101 ccgccctccg tgcgacactc gactgcctcg aaaacggcgc agactgattt cagacagcat
     2161 caaaacatga tgaaactcaa tccccaacag ctcgaagccg tccgctacct cggcggccca
     2221 ctgctcgtcc ttgccggtgc aggcagcggc aaaaccggcg tgattactca aaaaatcaag
     2281 tatttgattg tcaatgtcgg ctatctgccg cataccgttg ccgcgattac ctttaccaat
     2341 aaagccgccg cggaaatgca ggagcgcgtc gccaaaatgc tgcacaaatc gcaaacgcgc
     2401 gggctgacga tttgcacgtt ccactctttg ggcatgaaga ttctgcgcga agaggcgaac
     2461 catatcggtt acaaaaaaaa cttctccatt ctcgattcta ccgacagcgc gaaaatcatc
     2521 ggcgaactct taggcggtac gggcaaagaa gccgtattca aggcgcagca ccagatttcc
     2581 ttgtggaaaa acgatttaaa aacgcctgaa gatgtcgttc agacggcatc gaacatttgg
     2641 gaacaacaaa ccgcacgcgt gtatgcgagc tatcaggaaa ccttacaaag ctatcaggca
     2701 gtggacttcg acgacttgat ccgcctgcct gccgtgctgt tgcagcaaaa cagcgaagtg
     2761 cgcaacaaat ggcagcggcg gctgcgttat ctgctggttg acgaatgcca agatacgaat
     2821 acctgccaat ttacgttgat gaaacttctg accggcgcgg aaggtatgtt taccgccgtc
     2881 ggcgacgacg accagtccat ctacgcatgg cgcggcgcga acatggaaaa cctgcgcaaa
     2941 atgcaggaaa actatccgca gatgaaggtc atcaaactgg agcaaaacta ccgctccacc
     3001 gcgcggattc tcaaaatcgc caacaaagtc atcgagaaca accccaagct gttcaccaaa
     3061 aaactttggt cgaaattggg cgaaggcgag cccgtcaaag tcgttgcctg ccaaaacgag
     3121 cagcacgaag ccgactgggt cgtcagccaa atcgtcaaac aaaaactcat cggcggcgac
     3181 aaaacccaat atgccgattt cgccgtgttg taccgcggaa agcatcaggc gaggattttc
     3241 gaggaagcat tgcgcggcgc gcgcatcccc taccgtctct ccggcggaca aagctttttc
     3301 gacaaagccg aaatcaaaga cgtgttgtct tatgtgcggc tgcttgccaa ccccaatgac
     3361 gatcccgcct ttctgcgtgc cgttaccacg cccaaacgcg gcatcggcga tgtcacgctg
     3421 ggcaagctca acacttacgc gcacgaacac gaatgcagcc tgtatgaagc cgcacaaaac
     3481 gaagaagccc ttgccacgct gaacaatacc aaccgccaac acctgcaagc ctttatggat
     3541 atgttcggca gctaccgcgc caaagcggaa accggcgaag caggcgagtt catcaacagc
     3601 ctgctcgaag aaatcgacta tgaaaaccat ttgatgcaaa acgaagaagg caaggccggc
     3661 gaaatcaaat ggcgcaatgt cagcgatttg gtatcatggt ttgcgcgaaa aggcggggaa
     3721 gacggcaaaa acatcatcga actcgcccaa accgtcgcct tgatgacgct tttggaagga
     3781 aaagacgaag aagaaaccga tgccgtctcg ctatccacgc tacacgccgc caaaggtttg
     3841 gagtatccgt atgttttcct tgtcggttgc gaagaaggcg ttttgccgca caacgacagt
     3901 atcgaagagg gcaacgtcga agaagaacgc cgcctgatgt acgtcggcat cacccgcgcc
     3961 aaacgccaac tcacgctgac ccactgcgtc aaacgcaaaa aacaaggcat atggcagttc
     4021 cccgaaccca gccgattcat agacgaaatg ccgcaggaag atttgaaaat cctggggcgc
     4081 aaaggcggcg aaccgattgt cagcaaggaa gaaggcaggc gcaaccttgc cgatataatc
     4141 ggaaggctcg acaacctaaa aaaaagcggc gcggcggatt aaaccggagc cgcaatgccg
     4201 tctgaaggct tcagacggca tattttttgg acggcgcgcg taaagcggtt tacgcccaca
     4261 aatcctgctg ctggtttttc ggcacaagat gccccacgcc gataccgata aggcggaacg
     4321 cgtcttccgt ctgcggcggg acgcgcgcta tcaacatttg cgcagcctgc agcagagctg
     4381 cgcagtcggg caatacggag gaataagtca gtgtgcgcgt gatgatgcgg aaatcgtagg
     4441 tcttcagctt gagcgttacg ctttgggctt cgacgttttt gcgcgtgatt tgccgccaca
     4501 ggtcttcagc aagatggggc aggtgtccgg cagcctgctc gagcggcagg tcttcaggca
     4561 gggtgatttc tgtggagatt tggaggcgtt cgcgttcggc tttgacgggg cgttcatccg
     4621 taccgcgcac caaatcatag aggcggtatc cgtagcgtcc gaaatggttt aagagctcgc
     4681 cgcgctcgaa acggcgcaag tcgcccgccg tccgcatacc cagcgactgc atttttttca
     4741 gcgtaacctt gcccacgccg gggattttgc ccaaaggcag ggtttccaaa aatgccatga
     4801 ctttgtgcgg cggcaacaca aactgcccgt tcggcttgcg ccagtccgac gcgattttcg
     4861 ccagaaattt gttcggcgcg atgcctgcgg atgcagtcaa acctgtttcc gcaaaaatgg
     4921 cggcacggat ttctttggca acttcactgg cgtaagggat gtttttgaaa ttacgggtaa
     4981 cgtcaagata ggcttcgtcc agcgacaagg gttcgattaa atcggtataa cgcctgaata
     5041 cggcgtgaat ctgcgcggaa acctgacggt acaaatcgaa atgcggcggc acatacaccg
     5101 cttgcggaca cagccttttc gccgttgcca ccgacatcgc ggaatgcagc ccgaactgcc
     5161 gtgcctcata cgatgcggcg caaatcaccg aacgcgcgcc ctcccacgcg acgaccaccg
     5221 gccgcccttt caaatgcggc tgttcgcgca gctctaccga tgcgtagaac gcgtccatgt
     5281 cgatgtggat gattttgcgt gaagacatcg gctctgctga ggataaaagg gatattctac
     5341 tgccggcatc gggcaaattc caaatatact aacaggttaa atacaaaaaa gttggctgga
     5401 ttaattttgg aagcttataa aaataatggt ttttatcaag cagaaagaaa cagcaatgga
     5461 aattatgata tcgtaagaaa aaaattatct gaaaaaagat taccaaaata caagcaatat
     5521 attaatttac tcagataata atagtgactg atttaaaatt cagcagcgta gaaaacaaat
     5581 cagagcacaa atttcagcca gacaatggag aagataaaag atgaataaaa atatacttta
     5641 tatattttct ttattaatcg ctattgttat ttcttttata tttgaaaaga atgtaataag
     5701 caaaataagt tttaattata ataaaaaaga attct
//
LOCUS       DAWFBO010000128         5723 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFBO010000128 DAWFBO010000000
VERSION     DAWFBO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.128"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            35..1390
                     /locus_tag="WGO87_001629"
     CDS             35..1390
                     /locus_tag="WGO87_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0392969.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2413
                     /locus_tag="WGO87_001630"
     CDS             1394..2413
                     /locus_tag="WGO87_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0392970.1"
                     /translation="MSDTPATPATRDFGLIDGRAVTSYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYG
                     IESLS"
     gene            2647..4905
                     /locus_tag="WGO87_001631"
     CDS             2647..4905
                     /locus_tag="WGO87_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0392971.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALSGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPYDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGTLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHTAALEK"
     gene            4918..5583
                     /gene="pgmB"
                     /locus_tag="WGO87_001632"
     CDS             4918..5583
                     /gene="pgmB"
                     /locus_tag="WGO87_001632"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0392972.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGISIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVSEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVDA
                     DIRQCIGIEDAVAGVAAIKAAGALPIGVGKAEDLGSDIALVSGTAELTYAYLQNVWAQ
                     SGR"
BASE COUNT         1287 a         1641 c         1561 g         1234 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 aaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta ccaagaagcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccccgct acccgcgatt tcggcctgat
     1441 cgacgggcgt gccgtaacca gctatgtgct gtccaatcgg cgtggtacgc gtgtctgcgt
     1501 gctggacttg ggcgggattg tgcaggaatt ttccgttttg gcagacggcg tgcgcgaaaa
     1561 cctcgtggtg tcgttcgacg atgcggcttc ctatgcggac aatccgtttc agattaacaa
     1621 gcagataggg cgcgtggccg gacgcatccg cggtgcggcg ttcgacatca acggcaggac
     1681 ttaccgcgtg gaggccaacg aaggcaggaa cgcgctgcac ggcggttcgc acgggctggc
     1741 cgttacccgt ttcaacgcgg tggcggcaga cggccgttcg gtggtgctgc gcagccgcct
     1801 gcaacagtcg gccgacggtt atcccaacga tttggatttg gatatttcct accgcttgga
     1861 cgaggacgac cggcttaccg ttagctatcg cgccaccgcg ctcggcgaca cggtgttcga
     1921 cccgacgctg cacatttact ggcggctgga cgcgggcctg cacgatgcgg ttctgcatat
     1981 tccgcagggc ggacatattc cggccgatgc cgaaaaactg cccgtctcaa cggtttcaga
     2041 cgacctcgaa gtatttgatt tcagccggcc caagccgctg gatgccgccg ttgccgccct
     2101 gcgccgcgaa acgggtcggg ccggttttga cgacgcttac cgcgtgccgt ccgatatagg
     2161 ccgtcccgcc gctgtgttgc aagccggacg ccgccgtcgt atcagcatat acagcgaccg
     2221 caacggcttg gtcatcttta ccgccgcccc gcaggatttc gcgcggcacg atgcgggcgt
     2281 ttacgacgcg ctggcgaccg aggcgcagac gctgcccgac agcctgaatt ggcccgagtt
     2341 cggcaatatt cgtctgaaca agggtgatac tagggaggcg acgattgctt acggcatcga
     2401 atccctttct taggagcttc ctaacaccgg ttgcagacgg cctttttata gtggattaac
     2461 aaaaaccggt acggcgttgc ctcggcttag ctcaaagaga acgattctct aaggtgctga
     2521 agcaccaagt gaatcggttc cgtactattt gtactgtttg cggcttcgtc gccttgtcct
     2581 gatttttgtt aatccactat aagatttcac cattccctca aatcaatcca aacaggagct
     2641 tcataaatgt acacaagaat tatggaaatc agcccttgga cgctgcgttc ggcaaagctg
     2701 gaaaaagaac acaaacggct gcaagagagc ctgaccagtt tgggcaacgg ctatatgggt
     2761 atgcgcggca gctttgagga aacctattcc gccgacagcc acttgggtac ctatatcgcc
     2821 ggtgtgtggt ttcccgacaa aacccgcgtc ggctggtgga aaaacggcta ccccaaatat
     2881 ttcggtaaag ccatcaacgc gctcaatttc agcaaagtca aaatctttgt cgacggacaa
     2941 gaggtggact tggcgaaaaa cgatgttgcc ggcttctccg tcgaactcga tatgcagcac
     3001 ggcgtgttgc gccgctcgtt caccgtattc ggtgtgcgtt tcaatgtgtg caaattcctg
     3061 tccgtcgcgc aaaaagagct ggcggttatc cgctgggaag ccgtatccgt tgacggcaaa
     3121 acccaccaag tccgcatcga ttccatcatc gatgccgacg tgaaaaacga agactccaac
     3181 tacgaagaaa aattctggca ggtattggac aaaggcgttt cagacagtct ctcctacatt
     3241 gccgcccaaa ccgttgccaa ccctttcggc gtggaacaat ttatcgtcaa cgccgagcaa
     3301 actttcgccg gcagcttcaa agccctcagc ggcagccaaa ccgactggca agtctccaat
     3361 tctttcgagg tcgaagtcgg cggcacgccc aaaacctttg aaaaacgggt tattgttacc
     3421 accagccgcg attatcaggg cttggaagca gtgaaagccg caggccgcgc cttgtcggaa
     3481 aaagtcgcag gcgttgcgtt tgaaaccttg ctggacgcgc acaaagcagg ctggctgcac
     3541 cgttgggaaa tcgccgacgt ggtcatcgaa ggcaacgacg aagcgcagca aggcatccgt
     3601 ttcaatctgt tccaactgtt ctccacctac tacggcgaag acgcgcgtct gaacatcggc
     3661 ccgaaaggct ttaccggaga aaaatacggc ggcgcgacct attgggacac cgaagcctac
     3721 gccgtgccgc tctacctcgc actggcggaa cccgaagtta cccgcaacct gctgcaatac
     3781 cgccgcaacc aactgccaca ggcgcaacac aacgcgcgcg aacagggctt ggcgggcgca
     3841 ctctatccga tggtaacgtt tacgggcatc gagtgccaca acgaatggga aatcaccttc
     3901 gaggaaatcc accgcaacgg cgcgattcct tacgccatct acaactacac caactacacc
     3961 ggcgacgagg gctatcttgc caaagaaggc ttggaagtct tggtcgaagt gtcccgcttc
     4021 tgggcggacc gcgtccactt ctccaaacgc aacggcaaat acatgattca cggcgtaacc
     4081 ggcccgaacg aatacgaaaa caacatcaac aacaactggt acaccaacac cctcgccgca
     4141 tgggtattgg actacacccg cgaagccctg gcgaaatacc cgcgtccgga tttaaacgtg
     4201 cgtgccgccg agttggaaaa atgggcggac atcagcgcga atatgtaccg tccgtatgac
     4261 gaagaactcg gcgtattcgt acaacacgac ggattcctcg acaaagacat ccgccccgtg
     4321 tccgcgcttt cgcccgacga tttgccgctc aaccagaaat ggtcgtggga caaaatcctg
     4381 cgttcgccct ttatcaaaca ggcggacgta ttgcaaggca tctatttctt cggcgaccgt
     4441 ttcaatatcg acgaaaaacg ccgcaacttc gacttctacg aaccgatgac cgtgcatgaa
     4501 agctcgttgt cgccgtgtat ccactctatt ctcgccgccg aactgggcaa agaagaaaaa
     4561 gccgtggaaa tgtaccagcg caccgcccgc ctagacttgg acaactacaa caacgacacc
     4621 gaagacggcc tgcacatcac ctccatgacc ggctcgtggc ttgccatcgt ccaaggtttc
     4681 gcccaaatga aaacctgggg cggcacactc agcttcgccc cgtttctgcc gagcgcgtgg
     4741 acaggctacg ccttccacat caactaccga ggccgtctga ttaaagtcgc cgtcggcaaa
     4801 gaaaacgtcg tcttcaccct gcttaaaggc gaaccgctcg atttgcaggt gtacggcaaa
     4861 gacatcacgc tcgacggcag ccataccgcc gctttggaaa aataaggagg gcgcaaaatg
     4921 acgtttaccg ctgtgctctt cgatctcgac ggtgtcatca ccgataccgc cgaataccat
     4981 taccgcgcat ggaaaaagct cgccgaagaa ctgggcatca gcatcgaccg caagtttaac
     5041 gagcagctca aaggcgtgtc gcgcgacgat tcgctcaaac gcatcctcgc gcacggcggc
     5101 aaaaccgtca gcgaagccga gttcgccgaa ctgacccgcc gtaaaaacga caactacgtc
     5161 gagatgattc aggcagtcaa acccgaagac gtgtatcccg gcattttgcc cctgctggaa
     5221 acattgaaag caaacggcaa aaaaatcgcc ctcgcgtccg ccagtaaaaa cggcccgttc
     5281 ctgctcgaac gcatggggct gacccacttc ttcgacgcca ttgccgaccc tgccgccgtc
     5341 gcgcattcca aacctgcccc cgacatcttc ctcgccgccg ccgagggcgt ggacgcagac
     5401 atccgccaat gcatcggcat tgaagacgcc gtcgccggcg tcgccgccat caaagccgcc
     5461 ggcgccttgc ccatcggcgt gggcaaagcc gaagacttgg gcagcgacat cgcgctggtg
     5521 tccggcaccg ccgagctgac ctacgcctat ttgcaaaacg tgtgggcaca gtcgggcagg
     5581 taaaatgcgt cagataaagt gtcaaggaag taaaaggctg tctgaacagt gtttcagacg
     5641 gcctttctgc ttttatagtg gattaacaaa aaccagtacg gcgttgcctc gccttagctc
     5701 aaagagaacg attctctaaa gtg
//
LOCUS       DAWFBO010000129         5694 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFBO010000129 DAWFBO010000000
VERSION     DAWFBO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.129"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(197..409)
                     /locus_tag="WGO87_001633"
     CDS             complement(197..409)
                     /locus_tag="WGO87_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0392973.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(421..702)
                     /locus_tag="WGO87_001634"
     CDS             complement(421..702)
                     /locus_tag="WGO87_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392974.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(740..943)
                     /locus_tag="WGO87_001635"
     CDS             complement(740..943)
                     /locus_tag="WGO87_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392975.1"
                     /translation="MNIIEQEMKHSSLRKVDVEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1023..1595)
                     /locus_tag="WGO87_001636"
     CDS             complement(1023..1595)
                     /locus_tag="WGO87_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEZ0392976.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1574..1885
                     /locus_tag="WGO87_001637"
     CDS             1574..1885
                     /locus_tag="WGO87_001637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392977.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSVLSSVLPASLRRLRVGLMLTASSLRYSVARLMPNSCACCLRYAERAPRPIAS
                     A"
     gene            complement(1907..2782)
                     /locus_tag="WGO87_001638"
     CDS             complement(1907..2782)
                     /locus_tag="WGO87_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0392978.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2827..3117)
                     /locus_tag="WGO87_001639"
     CDS             complement(2827..3117)
                     /locus_tag="WGO87_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0392979.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3129..3308)
                     /locus_tag="WGO87_001640"
     CDS             complement(3129..3308)
                     /locus_tag="WGO87_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0392980.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3422..3799
                     /locus_tag="WGO87_001641"
     CDS             3422..3799
                     /locus_tag="WGO87_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0392981.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            3828..4073
                     /locus_tag="WGO87_001642"
     CDS             3828..4073
                     /locus_tag="WGO87_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392982.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4307..5275
                     /locus_tag="WGO87_001643"
     CDS             4307..5275
                     /locus_tag="WGO87_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392983.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5272..>5694
                     /locus_tag="WGO87_001644"
     CDS             5272..>5694
                     /locus_tag="WGO87_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392984.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNL"
BASE COUNT         1342 a         1290 c         1427 g         1635 t
ORIGIN      
        1 gcgaaaacag ctgaaaacct gtgtttgggt ttcggctgtc gggagggaaa ggaattttgc
       61 aaaggtctca gagtgagttt attttggggc ggcggcaggt cgggggcaag cggcgtgggg
      121 cttggttgtg gtttttaggt ttgggggtgg gtaaaaaatg ccgtacccgg cgggcaggct
      181 tcgcactctg aaggtttcag acggcgtttg ttttttctgt ccaatcgagg aactgccgcc
      241 atttttccag cggcatatcg gcccgccgcg tttcggtatc gggttcggct tcccagcggc
      301 ctacctgtat gtagtgcttc accccgacga tttgcgccaa ctcggcctgt gtcagtttgc
      361 agcggttgcg cagggtgcga aggttgtaag gcgtgtagcc gagttccatg tcgttgcggt
      421 ttattttgtt tcgcatattt ttttgactgc ccggcggcag gtttcggtaa ggacggcggc
      481 aaatcgggct tcgtctgccg gttttccgtc gaaaaaaata aaatcgtaaa gggtgatgcc
      541 gttttcggaa tggcggtaat gcccgcccgg gcattcgccg tctgtctgcc tgtggatttt
      601 ggcaatcagg cggggatagc ggcaatgggt aatgaaacgg cttgcgccgt cttgcccgat
      661 aatccattcg gggtagcggt tgaatagtgc ggctctgttc attttgttcg ggggataaag
      721 cccctcgcgg ggcttgtggt caggcaaatt tgaatatcag tgccaacacg gcggcgatgg
      781 cggtaaccag cccggtggcc gccatcatgg gataccatcg ggattcttgg gctattttta
      841 cggattcggc gttgattttg tgcgcgtctg caatgatttt ggcgatttcg acatctactt
      901 ttctcagact ggagtgtttc atttcttgtt cgattatgtt catcgtactt cctttcgttt
      961 ttggcggttg ccgccgcttg tcggatggca ggatgtctgc catgtgtata tattgatacc
     1021 ttttaggttt tattgcaagt gttttgggcg gcggcttcgt atgcttggcg gtggcggcgg
     1081 ctgtaccatt cggcgagttc gcgccgctct tggaggcggt agctgtcggc ggtcaggtag
     1141 tggtgcaggc ttgagaagcc ggcgcgttgg agggcggcgc ggctgatgtg gccgagggcg
     1201 aggtctgttg tgagggtgtc ttttccggcg gtcaggctga tgttggtgaa gaggtggcgg
     1261 aatccgtgca ttgtgtgttt ggatttgccg ggggtgctgc cgtcatagcc cagtcgtcgg
     1321 atggcgttgt gggcgaattt gatgctgatg tggtcgggat ggggggcggg tttgcgccgt
     1381 gggcggatgc cggggaaaag gtggatgttg tcgccggtct gtgtgtgcag ctctcggagt
     1441 atttctaccg cccagtccga cagtgggacg gtaaaggggt gtttggtctt catgtcggcg
     1501 gcggggatgt gccataaccg ggcggtgagg tcgatgtctt gccagcgggc ggaaagcagt
     1561 gcggacggac gggatgactt ccccggtttc cccatcaatc cgcgccgcct cgatttttcc
     1621 gttggcgcgc agcatttcca ccgcctgccg caccgacatc aaccgcttgc cgttgcgcct
     1681 catcgcctcc accaaaaccg ccgaaatcaa tttggcttct tccgttttaa gctccgtctt
     1741 gcccgcatcg ctgcgccgtt tgcgcgtcgg cttgatgctg accgcctcca gcttgcggta
     1801 tagcgtggca aggctgatgc ccaattcctg cgcctgctgc ttaagatatg cagagcgtgc
     1861 gccgcgtccc attgcttccg cctgattctc gactgcctta agacgctcaa tcattgccgg
     1921 attcatcgcc ttctcccgtt tcaccgccca accattccgg cacattgtct gtcggtgctt
     1981 cagtcggcag ggcatagctt tcgcgcagtt gctcgcagtc caaaataatt tgattgagcg
     2041 tgccgaccat ctttgcctga tggctgatct cgtgtgcctc actgtgcgca ttaagttggt
     2101 cgaacaaatc tttcaaacgg ctcacttgac tgcggatacc gacctcaagg cttgttaact
     2161 gcatcgccaa ctcactgccc acgtcttccg ccttcggctc tctgacaacg gtttgcttct
     2221 tagccagctt ctcggtcagc tcatcaattt tggctgtttt ggttttcatc acttcgtctt
     2281 tggcggcgag gttttcacgg ctttcgcgca gggcgacgcg cagctcgcgc accgtcattc
     2341 ggtccacatc gtcaaaggtc atgccgttga cttcttctcc ttcggcaagt cccaccaacg
     2401 taacgtcttc ttcgaccagc agctcaagca gcttcgactt gcccaaatcc atcagcttcg
     2461 gcgcggcttt ctgcatttgc ggggtcgcaa agcggcgagt ggctgacatc agacgtgatg
     2521 tttctgcgat acccagccca aattggcttt tcacaatttc cataaaccgt ccgtgttccg
     2581 tatgctcttt taaaataatc agcgcacgtc ccagttcgaa catgccttcc atcgtctgcc
     2641 gtactgcttg acgaccgcgt tcaacccaac gctcttcgct ataagtctcg ccgttacccc
     2701 actgctccat caccagcaca ctgcgcatcg ctgcctgatt gcttactttg tcggttgcga
     2761 taatttcaat ttctgtattc attttttatc tccaaagttt ccgacgtcgg aaactttcaa
     2821 aatccgttaa tccacatcga cccgcttgcc gatttcggca atcttgcttt gcagccgttc
     2881 atgctgctgc ctgaaccgct cggcgatttg cagggttttg atgccgtagg cgtagttgcc
     2941 gttttcaagt ttgatgacca atcccgaggc aaccaaatca tcaatatccc tgctgacttg
     3001 cgatggcgtc agccccagtc cgaccgataa atccttattg ctcagaccga taatcggatg
     3061 ctcgtcaagc gcgataaaga ccctcaatag ccgttgtacc cttttacttt ctgccatccg
     3121 catcctcctt atttcagtcc cagcttcttg gcaatttcat gccccttgcc gtaattgcct
     3181 ttgcgctgtc cgccgaccac cagatacaca tcgcgcggct taaagccgtt ctctcttgcc
     3241 cactgcgcca gcgtttggcc gttttgggca aaattttctt ttagtttttc aacagtatag
     3301 ccatagatag ccctttccca ttgtgataaa atagtgaact atttatgcac tagaaattaa
     3361 ttagtgcatt accaatgcgt gaattatgag attttaaaat ctcttttaca aggattattt
     3421 tgtgatttct aaatctcttt ttggtaatcg tttgaaagaa aaaagaaagt ttttagggct
     3481 tacccaagca caagctgctg aaaaagcagg tattgagcgc gaaacatggg gcaaatacga
     3541 acgtggcgta tttatgccta gtggagatgt attgctctct tttttgaata tgggaataga
     3601 tgtgagcagc ttatttgctg ctgaacaggg aattaggccg tctgaaaatt ctgaaattag
     3661 taaggaagag tcagagttat tagggtatta ccgccaagcc agcgataacg gcaaatttgt
     3721 cattctcagt gtggcaaaag gcgcagagaa aaaagcagcc gaaaatactc aagtttcagc
     3781 cacgcataaa acagcgtgat ttttaaatcc aaaatggaga cagtcttatg tttgactttg
     3841 aacaacaaat caaatggggt gagcgagcgg aagaaatcgt taaagaggca gccacccaga
     3901 acaatatcga aattcccgaa cctttggctt cggcattagc taaggcggta aaagtgcatt
     3961 atctaagcca agctggtgtg ttttctttgg tagaagccta tgcagacacg gtaaatccaa
     4021 ctgaaaaaga agtagattac caagcaatcg gcaaagagct atttgaaaaa taaaaaatgc
     4081 cgtctgaaat tttcagacgg catttaggac actccgacag tcgggctgct ccggtgttct
     4141 gaaacggata ttaagcaatt gaagcggccg tgcattttaa aacgcattaa aaactacccc
     4201 gacatacagg tcatcgggca ggtggttcag gtgttgaaag atttgaattg atgccgtctg
     4261 aatttatttt ttcgacacag atattaaaag agaaaggact gtacaaatgg caaaaaaaac
     4321 tactaaaact attcattatt tacgtgcgca gcaaaaccat agcttgttcg atttagaggt
     4381 agcattaaga agggttttgt cagctgctcc tactgttcaa gatacacaaa ttgaacaata
     4441 caatcagctt acccaaatca tgcatagaac tttggatcct aaagatgtaa aaggcaattc
     4501 aattggtgga ttactgatac acattggcag cggtacaaaa gatgaacata tccgcactat
     4561 gagcaataga cctgtacagc aagatgatca tggtggaaca caagctcctc caagtggata
     4621 ttcctttttg cgtaaagagg cttttttgta tattgttgga caccatgtaa ttttttgcgg
     4681 acatggtttt ttgtcagcat caacggttgc ttcttattta agtttactta gcaataaatt
     4741 gagagagaac aatccggata ttgttcaatt taatattgaa ttcaaagcag taggcaattg
     4801 cgacaagttg tcattgatac aacaacatgg ggtaaaaagt attcttttag atgcttcggc
     4861 atatcaatta tccagaaaca gactgtatca agatagccgt tcaaccattg ctaaagcatt
     4921 agggaaagtt ggtagtgtat ttacaagcga attgagtgat gaggaattgg aagcacaatc
     4981 cgaaattcat atcaatttag aagtcttgct aaacggtaat tcgagagcca gtattgaagc
     5041 gcaatcgctg atgcaggagc aggcagagga aattattgat gatgaaacgg ttaatcaagg
     5101 attttccatt accacgcaac aaggtgaagt gattcagccc tctgatgtaa aactgtctaa
     5161 atctgttaga ataggacgat acgatgaagc caactcgtta ctccctgact cggcatttac
     5221 agcaatcagt gaatatttcc tagaactcca aagcagaaac ttaactgaac aatgaaaaaa
     5281 tatctgccct tactacgttt tttgttgatt gcttgtatat cagcattttt agcatggaaa
     5341 gggcaacctt ttgttcatgg gaacgagaag gccgttgatt taattattaa cgtatttgca
     5401 atcttagccg gttttttaat tgccattatg acattgttta gtgatatgag gtttgatgaa
     5461 gatgcaaatt ggcgacaaat tcaaattcgt gaaggcgtac aagaacaacg gtatataaaa
     5521 cattccttac ttttttacac atatcttgcc gtattagttt gtgtttttat tgtcatcttg
     5581 ttggctcata aagaagaata caaaaatgga ccagccattt tttggcttga acggagctat
     5641 ttatttttag cctgtatttc cattttttat tctgtattct tacctgggaa ttta
//
LOCUS       DAWFBO010000130         5692 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFBO010000130 DAWFBO010000000
VERSION     DAWFBO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.130"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            97..813
                     /locus_tag="WGO87_001645"
     CDS             97..813
                     /locus_tag="WGO87_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0392985.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="WGO87_001646"
     CDS             962..1864
                     /locus_tag="WGO87_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0392986.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="WGO87_001647"
     CDS             1907..2680
                     /locus_tag="WGO87_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0392987.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="WGO87_001648"
     CDS             2792..4480
                     /locus_tag="WGO87_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0392988.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="WGO87_001649"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="WGO87_001649"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0392989.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWQLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1323 a         1541 c         1578 g         1250 t
ORIGIN      
        1 atttgtgcaa tgctttaatt cctctgacac gatttgaaac acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgtcagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc agatttgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgataa ggcgcgaaga agaggcttcc ggcaggggcg accgcgtcgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggaacgct tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca tatatcatgc ggcaataaag gaggtcagga
      961 gatggacagc ctgatgacat tgctttcggt attggtaccg atgttcgccg gattttttat
     1021 ccgtgtgccc aagccttacc tgcccgcttt ggacaaggtg ctgtcggttt tggtgtatgc
     1081 cgtgctgctg ctgatcggcg tatcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggcgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcattcgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaagcgcg ggcatgtatt gtctgatgct
     1381 gctggtgttc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcgggcgg
     1501 gctgctgttt gccgcatcga cagacggtgt gtcgtggacg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtcatgacc gaggcttacg gcgcggtatg
     1621 gggcagcatc atgctgctga acgatttggc acgagagctg tttgcactgg catttatccc
     1681 gctgctgatg aagcgttttc cggatgcggc ggtgggggtt ggcggtgcga ccagtatgga
     1741 ttttacattg cccgtgattc agggtgcggg cggtttggaa gtcgtgccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgcccc gtttctgatg gtggtgtttt ccgctttggg
     1861 ttgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgagttgga
     2041 ggaggccggc agcctgaaac gccatcacgg cggcgcatct tcgggcggaa acttgccgga
     2101 gggcctgccc gccgaccgcc aaacccggtg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa cacatccccg acggttcgtc gctgtttgtc agtatcggca cgacgatgga
     2221 agccgtggca tcggagctgg tcaggcggcg cggcagcctg cggattatta ccaacaatat
     2281 ccacgtcgcc tccatcgttt cggcgcggac ggattacacg gtcattatca cttccggagt
     2341 cgtccgccct ttggacggcg gtattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaatgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgcc tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat ttgcggacaa
     2821 ccattttatc cgcaccttca tcgaagaaga cctcaaaagc ggcaaacacg aaaccatcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggcgt atatttacga cggcttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggct
     3061 cggtttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttca cgcttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcgccgacgc ggctacacgc ccgaagggct
     3721 gcgcctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaattccgcc ccacgcctga tggcggtgtt
     3841 gaacccgctc aaagtgaccc tgaccaactt tgaaaccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aggaaatggg cgagcgcgaa gtacctattt cacaaaccat
     3961 ctatattgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tccagaaggc cgtaaagtta aaggcgtgat tcattgggtt tccgccgaac acgcagccga
     4201 aatcaaagtc cgcctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagatggc gaatacctgc cgtttaccga tttcctcaat ccggaatccg ttaaggaaat
     4321 cactgcttac gccgaacctg ccgcgaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgcaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ctgccgtctg
     4501 aatattgttc ggacggcatt tctcctttac ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctat
     4621 aaagacacca cctacgcgat gatgcgcgag atggcaaaac gcggctggca gctgtttcat
     4681 accttgagca gcggattgtc ggttaaaaac ggtctggtgg ttgctgatgc gtctgctttt
     4741 gagtttctcg gcgcacaaga cgaccacgac aaaaactggt ttgccgccgc cgacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccacaca gcttctgacg ctggcggagc agcagggcgc gaaggtcttt
     4921 aacagcggac aggcgatgcg tgatttcaat gaaaaactgg cgattttgaa tttcagccgc
     4981 tttaccgcgc ccacgctggt aacgacccgt tccgccgatg tccgcgcatt tttgaaagaa
     5041 cacggcgaca tcatcattaa accgctcgac ggcatgggcg gtatgggcat cttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcggct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg gcgcggcgtg gcgcaggaat tgggcggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgcttgc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatt
     5461 atgaaacaaa aaggtttcga cgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgtcgt ctgaaaggct tttgcttcac aaccgtttgg tttgatcggg
     5581 caggcagggt tttccctcag ccggcagaga tacgcttaat gccgtacgga caccccgccg
     5641 ccccgttttc agacggtata tatagtggat taacaaaaat caggacaagg cg
//
LOCUS       DAWFBO010000131         5652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFBO010000131 DAWFBO010000000
VERSION     DAWFBO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.131"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(129..890)
                     /locus_tag="WGO87_001650"
     CDS             complement(129..890)
                     /locus_tag="WGO87_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392990.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            990..1241
                     /locus_tag="WGO87_001651"
     CDS             990..1241
                     /locus_tag="WGO87_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392991.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1293..2099
                     /locus_tag="WGO87_001652"
     CDS             1293..2099
                     /locus_tag="WGO87_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0392992.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2406..3929
                     /locus_tag="WGO87_001653"
     CDS             2406..3929
                     /locus_tag="WGO87_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0392993.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            4027..5439
                     /gene="trkA"
                     /locus_tag="WGO87_001654"
     CDS             4027..5439
                     /gene="trkA"
                     /locus_tag="WGO87_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0392994.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1415 a         1704 c         1369 g         1164 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttaaatt taatccacta tattaatatt ttgcgcctgt
       61 tctatgatgc tttcaagtcg ggtgagaatg caaatgccgt ctgaaacggc tttcagacgg
      121 catggcaatc agcgtttgta ttttaactcg tacttgatgt cgttgaggat tttgcggaca
      181 tcgtgttcca acacgtcttc gactaccgca cccgcctgct cgtgcagcat ctgctggagc
      241 tgataggtaa aaaccgccat ctgcttttgc accgccgtac ggatgatgcc gttgacggta
      301 tcggtcagat gcgggcgcag gcgtttgatc agccgttcgg tcagctcctg ttcggacagg
      361 cagaacactt cgcgccggtt gacggctttc ggattcagga tattgatttg gacgggcatc
      421 aacgcctctt ccgcaccgcc ttcgccgttt tccgcaaccc cgtctgccgc cgtacccgat
      481 tctgccgcat cggcgttttc cccgccttca atctgtccgg tttcaaattc ggcactgtct
      541 tttttggcat cgaacaggat tctccgccgc gattcgatgt gtttttccga aaccgacatt
      601 tgcagggaag cctgcgcgtt gagccagttt tcctgaagga caatcatcgg gtcggtttcg
      661 acttcctcgc cgcaatcggc aacggcggca ttgtgttcct cctgccattt tttcagatac
      721 gccttcaaca cacgggctcg gctctcatcg tccagtttcg gcacaggcgc gtccgttccg
      781 gtttcagagg ggcgggacag cggcgcgtaa gtcggcactg ccttcatacg gcgcgtctga
      841 cgcaggtttt ccaaacgttt ttcccaattc ggctctttat tcgcatccat tttcggcttc
      901 cggtccttaa tctttgcaag cagacaaacc cgcgcccaaa gcgcggtttg atataatggc
      961 gcattttaac agattcgcga ggatacatca tgggcagcat cgaacagcgt ttggaatatc
     1021 tggaagaggc gaacgacgtg ctgcgtatgc agaaccacgt cctgtccacc gccttcaaag
     1081 ccttaatccg cgcccttccc gcagacaccg ccgaagtcgc ggtcgagtcg attcagcttg
     1141 catttgaaga cgcactggca gaattgagct atgaggacag cccgcatacg gatttgttcc
     1201 acgacgttac ttatgcgttt ttccgtgaaa aggaacgtta attttatgtt aaactgattt
     1261 tttaggcttt ttgattaccg aaaggaattt tgatgaatat gaaaaaatgg attgccgccg
     1321 cccttgcctg ttccgcgctc gcgctgtctg cctgcggcgg tcagggcaaa gatgccgccg
     1381 cacctgccgc aaaccccgac aaagtgtacc gcgtggcttc caacgccgag tttgccccct
     1441 ttgaatcttt agactcgaaa ggcaatgtcg aaggtttcga tgtggatttg atgaacgcga
     1501 tggcgaaggc gggcaatttt aaaatcgaat tcaaacacca gccgtgggac agccttttcc
     1561 ccgccttgaa caacggcgat gcggacgttg tgatgtcggg cgtaaccatt accgacgacc
     1621 gcaaacagtc tatggacttc agcgacccgt attttgaaat cacccaagtc gtcctcgttc
     1681 cgaaaggcaa aaaaatatct tcttccgacg atttgaaaaa catgaacaaa gtcggcgtgg
     1741 taaccggcta cacgggcgat ttctccgtat ccaaactctt gggcaacgac aacccaaaaa
     1801 tcgcgcgctt tgaaaacgtt cccctgatta tcaaagaact ggaaaacggc ggcttagatt
     1861 ccgtagtcag cgacagcgcg gtcatcgcca attatgtgaa aaacaatccg accaaaggga
     1921 tggacttcgt taccctgccc gacttcacca ccgaacacta cggcatcgcg gtacgcaaag
     1981 gcgacgaagc aaccgtcaaa atgctgaacg atgcgttgga aaaagtacgc gaaagcggcg
     2041 aatacgacaa gatttacgcc aaatattttg caaaagaaga cggacaggcc gcaaaataag
     2101 cccgcccgtc cgaacacaat gccgtctgaa gccctttcag acggcattgt tcatcaatcg
     2161 gcctacaatg aactgcctgc tgatttctcc ctaccgcaaa gcaacaggca aagattacaa
     2221 atatcaaaat ccgagtaaaa cagtatttta ttaaaacaaa ttgataatca agagattaga
     2281 attatgtatt gtctttaccg tacaaacgct ggcactattt caacctgata aaaaacagcc
     2341 ttcaaaaagg ttgtttaaaa cagcagcaga cacttaccgc cacaaccttg aaaaggaaca
     2401 caatcatgac cgtcatcaaa caggaagact ttatccaaag catttgcgat gccttccaat
     2461 tcatcagcta ctaccatccc aaagactaca tcgacgcgct ctataaagcg tggcagaagg
     2521 aagaaaaccc cgccgccaaa gacgcgatga cgcagatttt ggtcaacagc cgtatgtgtg
     2581 cggaaaacaa ccgccccatc tgccaagata ccggtatcgc aaccgtgttt ttgaaagtcg
     2641 gtatggatgt gcagtgggat gcggatatga gcgtggaaga aatggttaac gaaggcgtgc
     2701 gccgcgccta cacttgggaa ggcaatacgc tgcgcgcttc cgttctcgcc gaccccgccg
     2761 gcaaacgcca aaataccaaa gacaacacgc ccgccgtcat ccatatgagc atcgtgccgg
     2821 gcgacaaagt cgaagtaacc tgcgcggcaa aaggcggcgg ctctgaaaac aaatccaaac
     2881 tcgccatgct caatccttcc gacaacatcg tcgattgggt attgaaaacc atcccgacca
     2941 tgggcgcggg ctggtgtcct cccggcatct tgggcatcgg catcggcggc acgcccgaaa
     3001 aagccgtgtt gatggcaaaa gaatccttga tgagccacat cgacattcaa gagttgcagg
     3061 aaaaagcagc gtcaggtgca gaattgtcca ccaccgaagc cctgcgcctc gaactctttg
     3121 aaaaagtcaa cgcgctgggc atcggcgcgc aaggcttggg cggtctgacc accgtgttgg
     3181 acgtgaaaat cctcgattac ccgacccacg ccgcctccaa accgattgcc atgattccga
     3241 actgcgccgc cacccgccac gtcgaatttg aattggacgg ctcaggccct gtcgaactca
     3301 cgccgccgcg cgtcgaagac tggcccgatt tgacttacag ccccgacaac ggcaaacgcg
     3361 tcgatgtcga caagctgacc aaagaagaag tggcaagctg gaaaaccggc gacgtattgc
     3421 tgttgaacgg caaaatcctc accggccgcg atgccgcaca caaacgcctc gtcgatatgc
     3481 tcaacaaagg cgaagaattg cccgtcgatt tcaccaaccg cctgatttac tacgtcggcc
     3541 ccgtcgatcc ggtcggcgat gaagtcgtcg gtccggcagg tccgaccaca gccacccgca
     3601 tggacaaatt cacccgccaa atgctcgaac aaaccgacct cttgggcatg atcggcaaat
     3661 ccgagcgcgg cgtggccacc tgcgaagcca tcgccgacaa caaagccgtg tacctcatgg
     3721 cagtcggcgg cgcggcgtat ctcgtggcaa aagccatcaa atcttccaaa gtcttggcgt
     3781 tccccgaatt gggcatggaa gccatttacg aatttgaagt caaagacatg cccgtaaccg
     3841 tcgccgtaga cagcaaaggc gaatccatcc acgccaccgc cccgccccaa tggcaggcga
     3901 aaatcggcat catccccgtc aaatcttgag gcgtcatgcc gtctgaacac aaaatctgct
     3961 ttcagacggc atttccgccc ctggttgcgg tacaatccac catttcatca ctcggcgacc
     4021 cacaccgtga aaatcctcat tttaggcaac ggacaggtag gttctaccgt cgcacaaaac
     4081 cttgccgcca tacccaacaa cgacgtaacc gttatcgaca tcgacgaaaa agcattgcag
     4141 gaaacaggca gccgcctcga tgtccaaacc gttttcggca acggcgcatc ccccttcaca
     4201 ttagaacgcg ccggcgcgga agatgccgac ttgctgctcg cgctctcccg cagcgacgaa
     4261 accaacatcg tcgcctgcaa agttgccgcc gacctgttca acatccccgg ccgcatcgcg
     4321 cgcgtccgtt ccagcgaata cctcgaatac ctcagcccca agctcgaaaa caacgaaaac
     4381 ggcagccttt ccatattcgg cataaccgaa accatcagcc ccgaacagct cgttaccgaa
     4441 cagcttgccg gcctgataga ctgcccgggc gcattgcagg ttttacgttt tgcagacgac
     4501 cgcgtgcgga tggtcatcat acaggcgcgg cgcggcggac tgcttgtcgg acgcagcatt
     4561 gccgacatcg cccaagattt gcccgacggg gccgactgcc aaatctgcgc cgtttaccgc
     4621 aacaaccgcc tcatcgtccc cgcgccgcaa accgtcatca tcgaaggcga cgaaatccta
     4681 tttgccgccg ccgccgaaaa catcggcgcg gtcatacccg aattgcgccc caaagaaacc
     4741 agcacccgcc gcatcatgat tgccggcggc ggcaacatcg gctaccgtct cgccaagcag
     4801 ctcgaacacg catacaacgt caaaatcatc gaatgccggc cgcgccgtgc cgaatggata
     4861 gccgaaaacc tcgacaacac cctcgtcctg caaggttcgg caaccgacga aaccctgctc
     4921 gacaacgaat acatcgacga aatcgacgta ttctgcgccc tgaccaacga cgacgaaagc
     4981 aacattatgt ccgccctttt ggcgaaaaac ctcggcgcga agcgcgtcat cggcatcgtc
     5041 aaccgctcaa gctacgtcga tttgctcgaa ggcaacaaaa tcgacatcgt cgtctccccc
     5101 cacctcatca ccatcggctc gatactcgcc cacatccggc gcggcgacat cgttgccgtc
     5161 caccccatcc ggcgcggcac ggcggaagcc atcgaagtcg tcgcacacgg cgacaaaaaa
     5221 acttccgcca tcatcggcag gcgcatcagc ggcatcaaat ggcccgaagg ctgccacatt
     5281 gccgccgtcg tccgcgccgg aaccggcgaa accattatgg gacaccatac cgaaaccgtc
     5341 atccaagacg gcgaccacat catctttttc gtctcgcgcc ggcgcatcct gaacgaactg
     5401 gaaaaactca tccaagtcaa aatgggcttt ttcggataaa ccgccccatt ccggacacat
     5461 tgccgccaag cggtatggaa gcggaataat ggtaggtggg cttcagacgg catcgtcccc
     5521 caccgttatt cccgcgcagg cgggaatccg gtctgttcgg tttcagttgt ttttgggttt
     5581 cgggtaattt ccaaatcgtc attcccgcac aggcgggaat ccaaaccatt ggacagcggc
     5641 aatattcaaa gg
//
LOCUS       DAWFBO010000132         5630 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFBO010000132 DAWFBO010000000
VERSION     DAWFBO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.132"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(127..2259)
                     /locus_tag="WGO87_001655"
     CDS             complement(127..2259)
                     /locus_tag="WGO87_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0392995.1"
                     /translation="MSIASAPLPALSALIILAHYHGIAANPADIQHEFCASAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(3024..3707)
                     /locus_tag="WGO87_001656"
                     /pseudo
     CDS             complement(3024..3707)
                     /locus_tag="WGO87_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            4044..4478
                     /locus_tag="WGO87_001657"
     CDS             4044..4478
                     /locus_tag="WGO87_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392996.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4754..5473
                     /locus_tag="WGO87_001658"
     CDS             4754..5473
                     /locus_tag="WGO87_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0392997.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            5460..>5630
                     /locus_tag="WGO87_001659"
     CDS             5460..>5630
                     /locus_tag="WGO87_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0392998.1"
                     /translation="MSWNSFGNFMSARSSALAEFGNMVANLVSAKNEKDISKRNEYYK
                     QAGYSALLAFGNL"
BASE COUNT         1720 a         1214 c         1145 g         1551 t
ORIGIN      
        1 aagacagaaa aagtctttcc ggcagcctgc actttggttt catttcagtc agtaaaccca
       61 gtaaacgacg gtctgaaaac gcagaacgtt acgaaaaaag cagcctacac gcccatcccc
      121 cgccttctac ccgttctgta aatcatacag atagcggtaa tatccgttcg gcttcgccag
      181 caattcctgc tgtgttcccg cttccacaat cctgccttta tccatggcaa tgatccggtg
      241 tgccgtttta acagtggaca gacggtgggc gataatcagc accgtccggt tggcgcaaat
      301 ggcctgcatg ttctgcataa tcgctcgttc actttcataa tccagcgcgc tggtggcttc
      361 atcaaaaatc agaatgcgcg gattggtgat taacgcgcgg gcaatcgcaa tacgctgccg
      421 ctgtccgccc gacaagccgg ccccttgttc gcccaccacg gtgccgtagc cttccggcag
      481 ctccataata aactcgtgtg cgcccgccag tttggctgct tcgataatgc gttccagcgg
      541 catacccgta tccgtcagcg cgatattgtc gcgtatgctg cggttgagca gcacattctc
      601 ctgcaagacc acgccgacct gccgccgcag ccaagcagga gcggccaaag ccaaatcgtt
      661 gccgtccacc aacacccgtc cctgctccgg tacatacaga cgctgcacca atttggtgag
      721 tgtggatttg cccgaccccg aacgtcccac aatccccagc acttcccccg cccgaatccg
      781 caggttcaaa tcctgcaaaa tcagcctgcc gtccgcctta tagcggaaat cgacatgttc
      841 gaacgtaatc tccccccgga tatcgggcaa agccaaatgc gaagacgcat tctcggtcgg
      901 cgcattcaga atatccccca aacgcgccac cgaaatcccc acctgctgga aatcctgcca
      961 caactgcgcc aaacggataa caggcgccgc cacctgtccc gagagcatat taaacgcaat
     1021 cagctgcccc accgtcagct tgccctcaat taccagccgt gcgccaatcc acaacgtcgc
     1081 caccgtcacc agcttctgaa tcagctgcac cccctgctgg ccgaccaccg ccaacttcgt
     1141 tacccgaaat cccgaagcca cataagccgc caactgattg tcccaacgct gcgtcatctg
     1201 cggctccacc gccatcgcct ttaccgtacc caccgcagtg atgctttcta ctaaaaacga
     1261 ctggttgtct gcattgcgcg cgaacttatc gttcagacgc gtccgcagta tcggactgat
     1321 aaatgccgac caaaacgcat aggcaggcaa cgaagccaat accacccaag tcagagtgga
     1381 gctgtaatac cacatcaccg ccagaaagat aaacgaaaac gccaaatcca acaccgaagt
     1441 cagcgcctga ccggtcaaga aattgcgaat ctgctccaat tcccgcaccc gagccaccgt
     1501 atcacccact cgtctgtgct cgaaatagga taaaggcagg gaaagcagat gccggaacaa
     1561 acgcgcgccc aattccacat caatacgtga agtcgtatgt gcaaacagat acgtccgcaa
     1621 accgcccaac acaatctcaa acagcgacac caccaacaaa gccaccgaca ccacatccaa
     1681 agtagagaat ccccgatgta ccagcacctt gtccatcacc acttggaaaa acagaggcgt
     1741 aatcagcgca aacagctgca acaccaccga caccaccaat acttcaaaaa acaaccggcg
     1801 gtatttgatt accgccggaa taaaccaggt aaagtcaaac tttgccaaac tgcccaatac
     1861 cgaagcgcgg gaagcaacca atatcagttt gcccgaatat ctgttagaaa attcggcaaa
     1921 agacaatacc gcagacttat tcgtagttaa atcctgtatt agatattggg catgctcacc
     1981 cccaccgtct gttttagcca aaataaaatg gttgccgtca tcacaccata ccaatgcagg
     2041 taaggtcgcc atagccaaac gtttgacagg ctggcggact acctttgcct tcaatcccaa
     2101 agatttggcg gccaaaagcc attgtgtttc atttaaatcg ctttgtgcgg aagcacaaaa
     2161 ttcatgttgt atatcagcag gattggcggc aatgccgtgg taatgggcga ggatgatgag
     2221 ggcggaaagg gcggggagcg gtgcggatgc gatagacata aataaaatat agttagattg
     2281 gatgtggata acggctggct ggaaaaggaa tatattaagt agaaagaaat atataaataa
     2341 aacagcagaa cgcattgtaa ggatatatat gggaattgta aagagaaagt atggaaaagt
     2401 tctcgtttca ggaaggtaaa acggcttagg aatcgagtta gatgaggatg cctcgcacct
     2461 ctcgtgcctc ctgcataccg ttaaggcaca gggttaaggt gcaggctgct ccgaactctg
     2521 ttgcggtcgg gtaatgttat tttttgtgtt tcaggcagcc tgaaatatct gtatattttt
     2581 gttttaaata gattttaaag attgataact gttcttgacg atttttcaag aaaggagtaa
     2641 atttcaagaa aggagtaaag tgacttatta tcaatgacaa gcaacgcgcg aagtgacaag
     2701 gaaaactatc tacttaaatt ctaaggaggc ttcgaatatc ataaaccaat cagaaacata
     2761 gagataaaaa ttatgtacaa atataatcct cttatacaat ttattgcaca gttgattatg
     2821 tcttatggag caagcgtagg gtgggcactt gctgccccac gcgtttcata tttcaaggca
     2881 gcctgaaacc gtgtgggcat aaatgcctac cctacatccc aaaaaacaag cgcagcctgc
     2941 gtgtgtaggg tgcgaacttt cggcaggtag acacgcagtt ttatattttc aagctgaggg
     3001 atgcttaaga aaagtacaaa acattaaaaa ataaggggct gtactagatt agccctaaat
     3061 ccacaccaat cccgcaagat ttttagctgt cgggacggtg tgccgaagtt aaatcgaaat
     3121 tcgcattctt tcaagaacag cgggaaagat ttgcgatcaa ttccgttcta tttgcgcaag
     3181 acgcgttttg cctgattcca aaagttctca atgccgttta tgtggttctg acggtcagca
     3241 aattccttgg aatggttgat gcggtaatgg ataaaaccgc ttacgtccaa cttgtcgtaa
     3301 ctgctcaggc tgtcggtata aacaatgctg tccggcatga ttttcctttt gatgacaggg
     3361 agtaacgttt cagacctggc attatctaca acaacggtat agacccgtcc attgcgtttc
     3421 agaatgccga agacaaccac ttttcctgcc gcaccgcgac cgcgtctgcc tttacgccgt
     3481 ccgccgaaat agctttcgtc caactcgaca gagccctcaa aaacctcatc ggcagccaag
     3541 gccggataat ggctgatgac catacggatt gtgcggtaga acagaatagc ggagtcgggc
     3601 tggatgccca aaatatcggc agctgaacgg gcggtaactt caagtacaaa aaagcggagc
     3661 agttctttct gtactttttt ctttaatttg cagtgtgttg tcttcatatt tcgagggtaa
     3721 catatctgct aatctagtac agcctcaaac aaaaaagaga aattttaatt tcgctaaatc
     3781 gcataaagat taatcaagag tatcattaaa tgatatgagt gagcatctat aatgccaaga
     3841 agagttgtta agacataatg attattgaaa tagattgtaa aatagatact tagatagtct
     3901 gaaaaacgga tttgtgaaac tttttattac gcgccatcat ttgaaaatga aacttaaaaa
     3961 acacttatca taataaatat tttctttacg ttgtttgcta ataaactcag tgcaatatca
     4021 tcgcaatatt ttatggaaat tttatggata acaaaaaaga atttattaat aatttaacaa
     4081 ataggtatat gtggatctat ccattggtct taaatattct atttctacct ttttaccagt
     4141 cctaccaatc tttttttatt gcgcttggtt gtttgtttgc actggttaga aaaatgcaaa
     4201 gcttagattt taaattacaa aatcatattg tattgttaaa tataaaaagt gcttgggcag
     4261 ataaaaaagt atttttgatt aggatagtag tgtcatggtt ggcagtaatg gaaatatgga
     4321 tgtgttttat ttcggaatca tcaacgtggg tatgcggtgc tttttgttta aatagtgaaa
     4381 tattggaaaa aatttttcgt ggctttggtt attctggtag tttatatttt ttatttatat
     4441 tgatgattga tctcaataag ttaagagaga gtatttgaat ttcatttttt gtttgactta
     4501 aactcaagga gagtaacaat gattggtagt ggtgatacta aacaatgcaa aaaattttct
     4561 gcgtgtgatg gaaaatacca cgtctacgat cccctcgccc tagacttgga cggcgacggc
     4621 atagaaacag tcaccgccaa aggcttttca ggcagcctga agactgagag agtgaatacg
     4681 atgagtatac actctatgcc actaaattga tattcactaa atcataccag ctatatttta
     4741 tttaatgaga catatgaaaa ataaaaatta tttactagta tttatagttt tacatatagc
     4801 cttgatagta attaatatag tgtttggtta ttttgttttt ctatttgatt tttttgcgtt
     4861 tttgtttttt gcaaacgtct ttcttgctgt aaatttatta tttttagaaa aaaacataaa
     4921 aaacaaatta ttgtttttat tgccgatttc tattattata tggatggtaa ttcatattag
     4981 tatgataaat ataaaatttt ataaatttga gcatcaaata aaggaacaaa atatatcctc
     5041 gattactggg gtgataaaac cacatgatag ttataattat gtttatgact caaatggata
     5101 tgctaaatta aaagataatc atagatatgg tagggtaatt agagaaacac cttatattga
     5161 tgtagttgca tctgatgtta aaaataaatc cataagatta agcttggttt gtggtattca
     5221 ttcatatgct ccatgtgcca attttataaa atttgcaaaa aaacctgtta aaatttattt
     5281 ttataatcaa cctcaaggag attttataga taatgtaata tttgaaatta atgatggaaa
     5341 caaaagtttg tacttgttag ataagtataa aacatttttt cttattgaaa acagtgtttg
     5401 tatcgtatta attattttat atttaaaatt taatttgctt ttatatagga cttacttcaa
     5461 tgagttggaa tagttttggt aattttatga gcgcacgctc atccgcgtta gcagaatttg
     5521 gaaatatggt tgctaattta gtttctgcaa aaaatgagaa agatatctcg aaacgtaatg
     5581 aatattacaa acaagctggt tatagtgcat tattagcatt tggtaatttg
//
LOCUS       DAWFBO010000133         5580 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFBO010000133 DAWFBO010000000
VERSION     DAWFBO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.133"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            353..5518
                     /gene="iga"
                     /locus_tag="WGO87_001660"
     CDS             353..5518
                     /gene="iga"
                     /locus_tag="WGO87_001660"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0392999.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLANNERDANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTSHASTITLTGKSLITAPQNLSVYEIRNDYDDDDYYGYYSYRKP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGANLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEITVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALNSFGATQINGNV
                     NLSQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSHLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PVPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQTEEAKRQQAEAERKSAE
                     LAKQKAEAEREARELATRQKAEQERSTAELARRHEKEREAAELSAKQRAGEEERRQTA
                     QSQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAE
                     LLAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSAKQKAAAEAQ
                     ALAARQQKALARQQEEARKAAELAAKQKAETERKTAELAKQRAAAEAAKRQQEVRQAA
                     ELARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQ
                     DYMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDAFED
                     EDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRSQAAAQPQAQAAAQADAISTN
                     TNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRR
                     FSSKRTQTQIGIDRSLSENMQMGGVLTYSDSQHTFDQANGKNTFVQANLYGKYYLNDA
                     WYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLS
                     SADYKLGDDSVKVSSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVG
                     GNSFAYKADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1869 a         1348 c         1229 g         1134 t
ORIGIN      
        1 attaccacga aagcgggaat ctaggacgca gggttaagaa aacctacatc ccgtcattcc
       61 ctcaaaaaca gaaaaccaaa atcagaaacc taaaatcccg tcattcccgc acaagcggga
      121 atccaaaaac aaaaagccgc agaaatttaa cggaagaacc gaaccccgaa aaccggcttc
      181 ccgccttcgc ttgaataaca gaacaaagga agcggaaata aaaacatcgg gattcttttt
      241 ttaaattggc aataaaacac caaaattaat gatttacaca acaaaaatta gtcaacaccg
      301 ccaaaccggc gtaaaataca aaacattatc acccttaaaa cggtaaaacc ttatgaaaac
      361 caaacgtttt aaaattaacg ccatatcctt atccatcttt cttgcctatg cccttacgcc
      421 atactcagaa gcggcattgg tcagagacga tgtcgattat caaatattcc gtgactttgc
      481 agaaaacaaa ggcaaatttt ttgtcggcgc aaccgattta tcagtgaaaa acaaacaagg
      541 tcaaaacatc ggcaacgcat tatccaacgt accgatgatt gattttagcg ttgcagatgt
      601 caacagacgc acattaaccg tcatcgatcc ccagtatgcc gtcagcgtca aacatgtaaa
      661 aggagacgaa atctcttatt acgggcatca caatgggcac ttagatgtta gcaatgatga
      721 aaacgaatat cgttcagttg cacaaaatga ctacgaacca aataaaaatt ggcatcacgg
      781 taatcaaggt cgtcttgaag actacaacat ggcacgcctt aataaattcg taacagaagt
      841 cgcacctatt gcaccaacca gtgcaggcgg cggcgttgaa acctataaag ataaaaaccg
      901 tttttctgag tttgtgcgag ttggagcagg tacgcaattt gaatataaca gccgctacaa
      961 tatgacagag ctttcacgag cttatcgtta tgccattgca ggtacgcctt atcaagacgt
     1021 gaatgtcaca tcgaatctaa atcaagaagg cttgattggc ttcggtgata attcaaaaca
     1081 tcattcccca gaaaaactca aagaagtttt atctcaaaac gcattgacta actatgctgt
     1141 gttaggcgat agcggttcac cattatttgc ttatgataaa caagaaaaac gctgggtctt
     1201 tttaggtgct tacgattatt gggcaggcta tcaaaaaaac tcttggcaag aatggaatat
     1261 ctataaaaaa gaatttgcag atgaaatcaa acaacgcgat aacgccggca ccatcaaagg
     1321 ttatggcgaa catcattgga aaaccacggg cacaaacagc catatcggtt cgacagcggt
     1381 aaggcttgcc aacaatgaaa gggatgcaaa caacggacag aatgttacct ttgaaaacaa
     1441 cggcactttg gtattggatc aaaacatcaa ccaaggcgcg ggcggtctgt ttttcaaagg
     1501 cgattacaca gtcaaaggta taaataatga catcacttgg ctaggtgcgg ggattgatgt
     1561 taccgacggc aaaaaagtcg tttggcaagt caaaaatccg aatggcgaca gattggcaaa
     1621 aatcggcaaa ggcacattag aaataaacgg cacaggcgtt aaccaagggc aattaaaagt
     1681 cggcgacggt acggttattc tgaatcaaca agccgatgcc gacaaaaaag tccaggcttt
     1741 ctcccaagtc ggcattgtca gcggacgcgg tacattggta ttaaatagtt caaatcagat
     1801 taatcccgat aacctatatt tcggtttccg tggcggtcgt ttggatgcca atggcaacga
     1861 cttgactttt gaacacatcc gcaacgtgga tgaaggcgcg cgcattgtca accacaacac
     1921 aagccacgcc tccacaatca cattaaccgg caaatcatta attaccgctc ctcaaaatct
     1981 ttctgtttat gaaatccgaa atgattacga tgatgacgat tattatggct attacagcta
     2041 tagaaaaccc attccgcaag gcaaagatct ttattacaaa aactatcgtt attacgccct
     2101 aaaatccggc ggcagcgtga acgcaccgat gcccgagaac ggtgtaacag aaaataacga
     2161 ctgggtcttt atgggatata cgcaagaaga agccaagaaa aacgcgatga accacaaaaa
     2221 caatcagcgt attagcggtt ttagcggttt ctttggcgaa gaaaacggaa aaggacataa
     2281 cggcgcatta aaccttaatt tcaacggcaa aagcgcgcaa aaccgtttct tgttaacagg
     2341 cggtgccaat ttaaacggaa aaataagcgt aactcaaggc aatgtcttgt tatcaggtcg
     2401 tccaacacca cacgcaagag attttgtgaa caaatcttca gcccgaaaag acgcacattt
     2461 ttccaaaaac aatgaagtcg tatttgaaga cgactggata aaccgcacat tcaaagccgc
     2521 agaaattacg gttaatcaat ccgcatcatt ctcttccggc agaaatgtat ccgacatcac
     2581 cgccaacatt accgcaacag acaacgccaa agtaaatttg ggttacaaaa acggcgatga
     2641 ggtttgcgtg cgctcggact ataccggtta cgttacctgc aacacagaca acttatccga
     2701 taaggcttta aacagctttg gagcgacaca gattaacggc aatgtgaatt tgagtcagaa
     2761 tgcggcattg gtcttaggca aggctgcatt atgggggcaa attcaagggc aaggaaacag
     2821 ccgtgtcagc ctaaaccaac atagcaaatg gcatttgacc ggcgacagcc aagtacacaa
     2881 tctgtcattg gcggatagcc atattcattt gaacaacgct tccgacgcgc aaagtgcaaa
     2941 taaataccac acgctcaaaa tcaatcattt atccggtaac gggcattttc attatctgac
     3001 gcacctggcg aaaaatcttg gggataaagt gcttgtgaag gaatccgcat ccggtcatta
     3061 tcagctccat gttcaagata aaacaggcga acccaatcag gaagggctga atctctttga
     3121 tgcatcatcc gtacgagacc gttcgcacct ttctgtttcc ttggcgaata accatgtaga
     3181 tttaggcgca ttgcgttata caatcaaaac agaaaacggt attacccgat tgtacaatcc
     3241 ttatgccgag aaccgccgcc gagtcaaacc ggtcccgtct cctgccacaa acacggcttc
     3301 tcaggcacaa aaggcaacac aaacggacgg tgcacaaatt gccaagcctc aaaatatcgt
     3361 cgtcgcaccg cctagcccgc aggcaaatca aaccgaagaa gcaaaacgcc agcaagcaga
     3421 agcagagcgt aaatcggcag agttagcaaa acaaaaagca gaagccgaac gcgaagcaag
     3481 agagctggcg acccgccaaa aagcagaaca agaacgcagc accgccgaac ttgcacgccg
     3541 gcatgaaaaa gaacgcgaag cggcagagtt gtcggcaaaa caaagggccg gggaagagga
     3601 acgccgtcaa acagcccaat cccagccgca acgccgcaaa cgccgtgccg caccgcagga
     3661 ttatatggca gcttcccaag accgtcctaa acgccgcgga caccgttcgg tacagcaaaa
     3721 caatgtggaa attgcccaag cccaagcaga acttgcgcgc cgacagcagg aagaacgcaa
     3781 agcggcagag ttgttggcaa aacaaagggc agaagccgaa cgtgaggctc aagcattggc
     3841 tgcgcgccga aaagctgaag ccgaagaggc aaaacgccaa gctgccgaac ttgcacatcg
     3901 ccaggaagca gaacgcaaag cggcagagtt gtcggcaaaa caaaaagccg cagctgaagc
     3961 ccaagcattg gcagcacgcc aacaaaaagc ccttgcgcgt caacaggaag aagcacgaaa
     4021 agctgcagag ttagccgcca aacaaaaagc ggaaaccgaa cgcaaaaccg ccgaacttgc
     4081 caagcaaagg gcagcagccg aagcagcaaa acgccagcaa gaagttcgcc aggccgccga
     4141 acttgcgcgt cgtcaagaag cggaacgcca agcagcagag ttgtcggcaa aacaaaaagc
     4201 cgaaacagat cgagaagcgg cagaatccgc gaaacgaaaa gctgaggaag aagagcaccg
     4261 tcaagcagcc caatcccagc cgcaacgccg aaaacgccgt gccgcacctc aggattatat
     4321 ggcagcttcc caaaatcgtc cgaaacgtcg cggacgcaga tctactctgc cggcaccgcc
     4381 ctcgccatca tttgattcat cagcttacgc agcacccagg gccttgcata atccggactg
     4441 gtatgaaaat gactatgaag aaatcccctt ggatgcgttt gaagatgaag atgtatccga
     4501 atcggttgac acatcagaca aacagcctca agacaatacg gaacttcatg aaaaagttga
     4561 gacagtgagt ttgcaaccaa gagccgcgca gccgcgatcc caagccgccg ctcagccgca
     4621 agcccaagcc gccgcgcaag ccgatgcaat cagcaccaat accaactcgg ctttatctga
     4681 cgcaatggca agcacgcaat ctatcttgtt ggatacaggt gcttcattaa cacggcacat
     4741 tgcacaaaaa tcacgcgctg atgcagaaaa aaacagtgtt tggatgtcga acaccggtta
     4801 tggccgtgat tatgcttccg cacaatatcg ccggtttagt tcgaaacgca cgcaaacaca
     4861 aatcggcatt gaccgcagct tgtccgaaaa tatgcagatg ggcggagtat tgacttactc
     4921 tgacagtcag catacttttg atcaggcgaa cggcaaaaat acttttgtgc aagccaacct
     4981 ttatggtaag tattatttaa atgatgcttg gtatgtggcc ggcgatattg gtgcgggcag
     5041 cttgagaagc cggttacaaa cgcagcaaaa agcaaacttt aaccgaacaa gcatccaaac
     5101 cggccttact ttgggcaata cgctgaaaat caatcaattc gagattgtcc ctagtgcggg
     5161 tatccgttac agccgcctgt catctgcaga ttacaagttg ggtgacgaca gtgttaaagt
     5221 aagttctatg gcagtgaaaa cactaacggc cggactggat tttgcttatc ggtttaaagt
     5281 cggcaacctt accgtaaaac ccttgttatc tgcagcttac tttgccaatt atggcaaagg
     5341 cggcgtgaat gtgggcggta attccttcgc ctataaagca gataatcaac agcaatattc
     5401 agcaggtgcc gcgttactgt accgtaatgt tacattaaac gtaaatggca gtattacaaa
     5461 aggaaaacaa ttggaaaaac aaaaatccgg acaaattaaa atacagattc gtttctaaaa
     5521 tactaaattc atagcaaaat aaaatgccgt ccgaactcag gcttcagacg gcatttttat
//
LOCUS       DAWFBO010000134         5532 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFBO010000134 DAWFBO010000000
VERSION     DAWFBO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.134"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(241..2988)
                     /gene="tbpA"
                     /locus_tag="WGO87_001661"
     CDS             complement(241..2988)
                     /gene="tbpA"
                     /locus_tag="WGO87_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ0393000.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDTNQYANFIVEEECESKNYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKQAGSLSGNGKYAGDHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     DLRHQDYYYQNANRAYSSKIPTQNGNQKISPNGSQDKPYWVSIGGGNVVTGQICRSGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPVDWLDLTYRTSTGFRLPSFSEMYGWRSGDKIKAVKIDPEKSFNKEVGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINILG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
     gene            complement(3075..5216)
                     /locus_tag="WGO87_001662"
     CDS             complement(3075..5216)
                     /locus_tag="WGO87_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0393001.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRFKRRNWHPQANPKEDEKKLSENDWEATGLPSDPKNL
                     PERQKSVIDKVETGSDSNIYSSPYLTPSNHQNGNTGNSANLPKNEVKDYKDFKYVYSG
                     WFYKHAKQKIDFQNKIAQQGDDGYIFYHGKEPSRQLPASGTVTYKGVWHFATDTKKGQ
                     KFREIIQPSKNQGDRYSGFSGDDGEEYSNKNEATLQGGQEGYGFTSNLEVDFDNKKLT
                     GKLIRNDKVTNATTGDKHTTQYYSLEAQVTGNRFNGKATATDKPQNSETKEHPFVSDS
                     SSLSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTKDNTANGNTAAASGGAGAAASGGA
                     AAMPSENSKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGNTN
                     QGTNGGTAFTYATTYTPESDKKDTQAQTVTGGTQTASNTAGDANGKTKTYAVEVCCSN
                     LNYLKYGLLTRKTVGNTREGGNSSPTAAAQTAQGAQSMFLQGERTDEKEIPKEQNVVY
                     RGSWYGYIANDTSWSGNASDKEGGNKADFTVNFGEKKITGTLTAENRQAATFTIEGTI
                     QDNGFSGTAKTADSGFDLDQSNTTRTPKAYITDAKVQGGFYGPKAEELGGWFAYPGDK
                     QTENATVASGNGNSASSATVVFGAKRQQLVQ"
BASE COUNT         1153 a         1401 c         1385 g         1593 t
ORIGIN      
        1 ctttcgcggg aatgacggga tttgaggttt ctgtttttga ttttctgttt tcgcgggaat
       61 gacggtttag aagttacccg aaacctcaaa aaaacctaaa ccgaacggtc tagattcctg
      121 cctgcgcggg aatgacgaat ttcaagatta cggtattgtc ggggatgatg gaaaacggtg
      181 ggaattgtgt aaaaaatgcc gtctgaagcc tttcagacgg catttgcggc gtttggacgt
      241 ttaaaacttc atttccaagc taaatgtgta gtttcggccg ggggcggcat atcggttgta
      301 aacgccgaca tttttgtgtt gattgactgc gccgccggca gtttgccgca cattttccca
      361 agtaacatag cggtagttga ggaggttgta cacgcccgca cggagggtga agtgtttttt
      421 aatcgtgtaa taaccggaca catccacaat ataccaaggg cgggtacggc gcgcggtggc
      481 ttttgtattg cggctgttgc cgttgagcaa agcccggctg cccaacaact ctgtgatttc
      541 cttggctttg gaataagtca gcataccgtt cacaccccat ttgccttccg gttggtcata
      601 gcccaagccg acgacatagc gcgaaggttg gatggcatca aacagatgtg attgaatatc
      661 ggtgcggtct gcgcgttttt tgatgtcgcg gacacggaca cgattatagg caaatgtaga
      721 ataccaacct tcgggcaatt tatcccatac gccgttccaa tcgattttgc ccaaaatatt
      781 gatgccggta atccgcgcgc tttgggcatt gaggtaagcc gggtcgcctt tggctgtttc
      841 tttgccgttt tgaatttgcg cttcataacc ccggacaatc aaatcgcggt aggcattgtt
      901 gaaccaactt gcctccaagt tgccgaaatc gcctttaaac acgatgccga cttctttgtt
      961 gaacgatttt tccggatcga ttttgacggc ttttatttta tcgcccgacc gccagccgta
     1021 catttccgaa aacgagggca ggcggaagcc ggttgaagtg cggtaagtca aatccagcca
     1081 gtcgacaggt ttgaggacga tgccggcgtt ccaggacagg gtgcggtgcg tgccggtgga
     1141 aacgctgccg tcgtccgaat gcgtgctgcg gtagtcgtag cgcaagcccg cgccgacatc
     1201 cgcccacctg cccaaacgga cattgtcccg aactgccgcg taatagcttt tgccgttgat
     1261 gctgcgcggc gtgcagtccg tataagtatt gttgcccgag cggcagattt gccccgtaac
     1321 cacatttccc ccgcctatgc tgacccaata gggtttgtct tggctgccgt tggggctgat
     1381 tttttgattg ccgttttgag tgggtatttt cgacgaatag gcgcggttgg cattttgata
     1441 ataataatcc tgatggcgga ggtcggaacc aaaacggtca aaaccgaggt tgacgctcag
     1501 gttgtggcgg attttggcgg tatcgaagga ttttttgaat gccgcctgca agagcctgtg
     1561 gctttccccg taaatcacgc ggtcggattt gtaataggaa aacggcttgt cggcactcgg
     1621 gcggcaatat ttgtccgaac cgtcggcaga acagtgcgtc tgctgaaaat gattatccaa
     1681 accgataccc tgccggtcgt aagagaggcg ggcataatcc gcccaagtgt ctttatcggc
     1741 attggtatag acatattcca aaccgtagcg gcttttggtg tgcgtctcgt cgtaaaacac
     1801 gcccgtaccg tattccgcgc ccaccagcgc accgttttcg ccgttggtaa acagaccgcc
     1861 gtatttgtgg tcgcccgcgt atttgccgtt gccggacaaa gaacccgcct gttttttatt
     1921 ttcatcaaaa accgccttgg tcaggaatgc cggaaccgtc atatcgcgcg tgtcgaaagt
     1981 ttgttgcgtg cgttcgagta tgccgccgat gtagtgccgt ttgttttcaa aacgaaaacc
     2041 cgggcggaac agccacgacc ggctttcgta tgaaagcgga tcggcgagga agcggttggg
     2101 gcccgtgtag tctcgggtgg aaaccgtttg ccgttcgtct ttgccgacaa catctttttt
     2161 cggattcgct ttacacgttt cgtaattttt gctttcgcat tcttcttcaa cgatgaaatt
     2221 tgcgtactga ttggtatcct caaccggcac cagcctgtta aagctctgaa cgccgcgtcc
     2281 ggcggcttcg tgtgcgcgaa tttcccccgc gcgccgaccc gtgtggatca gcaaagcctc
     2341 cgcaccgccg atgcgccccg ccagcgcgat ggattgggta agcccccggt ttttgccgga
     2401 ataggcggtt ttactctgaa tcccccactg cctgccttcc ccgataacat cgtcggcggt
     2461 tttggtttga aatgcgaccg agcccgccaa tgcgccgctg ccttgttcga ccgagtttga
     2521 gcctttgctg atttcgacag ctttgacgtt ttcatactcg atttcattga ttgcgccgct
     2581 gctgcccgcc gtcctcgttc cgcccaatgc cgcctgcgcg gtgtaggact gtatttgcgc
     2641 caagccgtcc accgtcaagg aaacgcggtt tttgtccata ccgcgtatcg agtagcccga
     2701 gcttgcgccg cgcccttgtt cgacgacggc gatgccgggg tcgtaacgcg tcaggtcgcg
     2761 gatatcgagt acctgttcct tgctgagggt gtcggcggtt ttgaccaatt tgcccaaacc
     2821 ggttacttca ttatcgcggc gggttttctg ttttttggct tttacctgta tggtatccaa
     2881 ctgtttttcc tgtgcttgtc cggcttgcac attttctgca taaacgggca gcgcagtcat
     2941 taaagacagg cataaaatat ttaatcggaa caaatgttgc tgttgcatag tgtttcccta
     3001 atcttcgctt tcagacggca tcggaaggaa cgatgccgtc tgaagcctta ttctcgattg
     3061 ttcggcagcc gtgcttattg cacaagctgt tggcgtttcg caccgaatac gacagttgca
     3121 ctgcttgctg aatttccatt gccggatgca actgttgcat tttctgtttg tttatcgccc
     3181 ggataggcaa accatccgcc caactcttcg gctttgggcc cgtaaaaacc gccctgcacc
     3241 ttggcatctg tgatatatgc cttaggcgtg cgggtggtat tgctttgatc gagatcaaag
     3301 cctgagtcag cagtttttgc cgtaccggaa aaaccgttgt cctgaatcgt tccctcaatg
     3361 gtaaaggttg ccgcctgcct gttttcagcg gttaacgtgc cggtaatttt tttctcgcca
     3421 aaattcacgg taaagtccgc cttgttgcct ccctctttat ctgaagcatt gccgctccag
     3481 cttgtgtcgt tggcaatata cccgtaccaa gacccccgat aaacgacgtt ttgctctttt
     3541 ggaatctctt tttcatcggt acgctcgcct tggaggaaca tactttgtgc accctgtgcc
     3601 gtttgggcgg cggcggttgg gctgctgttg ccgccttccc tcgtattgcc gacagttttg
     3661 cgcgtcagca acccgtattt cagataattg aggttggaac agcagacttc gactgcatag
     3721 gtttttgttt tgccattggc atcacctgcc gtatttgaag cggtttgcgt gccgcccgtg
     3781 actgtttggg cttgggtgtc ttttttatca ctttccggcg tgtaggttgt tgcgtaggta
     3841 aaggctgttc cgccgtttgt accttgattg gtattcccac tttcggaagc ctcgggcagg
     3901 agcggaatca taatgccgtc gacaaccagt tgggcggcgt tgctgaagtt gtcgagattt
     3961 ttgattgctg tgccgccgtg cgtcagctcg accgcatcca aaaccgtggt cagcttactg
     4021 ttttcagacg gcatagccgc cgcaccgccc gatgctgccg cacctgcgcc gcctgaagcc
     4081 gccgcagtat tgccatttgc agtgttgtct ttggttttcg cgctgccgac aaccgcaact
     4141 tttttatcgt cgctcaaaaa gcggaaaccc aattcctcac ccttcgggcc gaaaaagccg
     4201 ccactcaaag aagacgagtc ggaaacaaag ggatgttcct tggtttcgct gttttggggt
     4261 ttgtcggttg ccgttgcctt gccgttgaag cggttgcctg ttacttgagc ctcaaggctg
     4321 taatattgcg tggtatgttt gtcgccagta gtagcgtttg taactttatc attgcgtatc
     4381 agtttgcccg tcaatttctt attgtcgaaa tccacttcta aattcgaggt aaaaccataa
     4441 ccctcttgac cgccttgtaa agtcgcttca tttttattgg aatattcttc gccgtcatca
     4501 cccgaaaatc cgctatacct gtcgccttga ttttttgaag gttggataat ttcacgaaat
     4561 ttttgaccct tttttgtatc ggtcgcaaaa tgccacacac ctttataggt aactgttcca
     4621 gaagcgggaa gttgtcggga aggttctttg ccgtgataaa agatatagcc gtcgtcgcct
     4681 tgttgtgcaa ttttattttg aaaatcaatt ttttgtttag catgtttata aaaccagccg
     4741 gaataaacat atttgaaatc tttataatct tttacttcat tttttggcag gtttgcactg
     4801 ttgccagtgt tgccgttttg atggtttgat ggtgtgagat agggggaaga ataaatattg
     4861 ctgtcgctgc ctgtttctac tttatcaata actgatttct gtcgctcggg taagttttta
     4921 gggtcacttg gtaatcccgt cgcctcccaa tcattttcag aaagtttttt ctcatcttct
     4981 ttaggatttg cctgcggatg ccaattccgc cgcttgaagc gcattgcaaa accgtatccg
     5041 ccttggtctt tttgggcttg cggtttttcg gaagaaacat cttgatactt tggcgcggga
     5101 cgcggggctt cggtatcgac agaatcaaga tcgaagctgc cgcctccgcc caaacaagca
     5161 ctcaacaaaa acacaggcag caccatagca gcctgattca ccaatggatt gttcataata
     5221 aatccaattc aattaaagaa tgataaggat tattatttta tttattttta acaaatttgc
     5281 aaatactttt ttatttttta gggaatacac caaaatcccg tcattcccgc gcagtcgtga
     5341 atccgaacgc gtccgcacgg aaacctatat cccgtcattc ccacgtatat cccgtcattc
     5401 ccacgtatat cccgtcattc ccacgtatat cccgtcattc ccacatatat cccgtcattc
     5461 ccacgaaagt gggaatccag gacgcaaaat ctcaagaaac cgttttaccc gatacgtttc
     5521 cgcaccgaca ga
//
LOCUS       DAWFBO010000135         5454 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFBO010000135 DAWFBO010000000
VERSION     DAWFBO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.135"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(337..2613)
                     /locus_tag="WGO87_001663"
     CDS             complement(337..2613)
                     /locus_tag="WGO87_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0393002.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHGTEQSVGLETISVVGKSRP
                     HATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINVGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHAHNGKPWIDLRNKR
                     YELRAEWKQPLPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENHYNYPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDNQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            3024..3614
                     /locus_tag="WGO87_001664"
     CDS             3024..3614
                     /locus_tag="WGO87_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0393003.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVVVRRNDDITLEEIEALKP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVIRAKTLMHG
                     KVSPVSHSGKGMFKDLPNPVTCTRYHSLVIARDTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3678..4736
                     /gene="trpD"
                     /locus_tag="WGO87_001665"
     CDS             3678..4736
                     /gene="trpD"
                     /locus_tag="WGO87_001665"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0393004.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKMPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4888..5103)
                     /locus_tag="WGO87_001666"
                     /pseudo
     CDS             complement(4888..5103)
                     /locus_tag="WGO87_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5205..5454
                     /locus_tag="WGO87_001667"
                     /pseudo
     CDS             5205..5454
                     /locus_tag="WGO87_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
BASE COUNT         1318 a         1235 c         1493 g         1408 t
ORIGIN      
        1 acctaaaact taaaaaaaca gaaacctttc cgcgtcattc ccacgaaagt gggaatctag
       61 aacccaaatg ctaaggcgat ttatcggaaa cgactgaaac cgaatgaatt ggattcccgc
      121 ctgcgtggga atgatgggat cttgggtttt tgcttttgat ttttctgctt ttgcgagaat
      181 gacggcgtga aagcaagaat gatgaaacaa aaaaaatggg aatgatggca tagtggtttg
      241 ttctttgtct ttgccatatt tcctaacaaa ttgattaaaa agaaaaaagg ttttcagaat
      301 gccgtctgaa aacctttttt gtttgcctgt ccgattttaa aacttcacgt tcacgccgcc
      361 ggtaaagctg cggcccattt gcggcgtatc agagagaaag ctgctgtggg cgtaaacgga
      421 ttggttgagc aggttgtcgg ctttgacgta ccaattccac tcgccatagc gcgtattgcg
      481 gcggtagttt gcgccgaggt tgagcatatg gtgtccgggc gtgcgcgttt cgtagcgggc
      541 gagtttgttt tgggcgaaca cgcggtagta gtccaaattg gcatcgatgc ggtcggtcag
      601 cgaggctttc aggtgaacgc cgaggcgcgc agccggaacg cgaggggcgt tttggttatc
      661 ctgtgcaatg aaaggacggt tgccgtaggc atcttccctg ccaggtaggg aaggcaggtt
      721 tttcagacgg cctcgtacat agtcgccgga aacgccgatg cggtagcgcg gtgtcggttt
      781 gaagtagatt tcgccttccg cgccgtagaa gtccgcaccg gattggttgt agcgcacgag
      841 cttcatttcg ctgtcgtctt cgatggattt ggggccgcgt ccgtcgttta aggtttgggc
      901 gtagatgtag ttgccgaaac ggttgcggta gagtgccaga ttgtattgcc agcggtcgcc
      961 ttcgtagccc agtgcgagtt cgatattgtt ggaacgttct ttgttgaggt gtttgttgcc
     1021 gacttcaaaa gtgttggtgg caacgtgttt gccgtgtgcg tacagctctt gcgttgacgg
     1081 caggcgttcc tgatgggagg cggtcaggct gagtttgtgg tgcggcgtga aataccagtt
     1141 gcccgaaagt gcgaacgagc gggcggtttg gcggtgtgcg ccgaggtcgg gcagggggta
     1201 gttgtagtgg ttttcccgat caatcaatgc tttgtcgtac cggatggagg ctttttgttt
     1261 ttccacgcgt acgccgcctt caagcgtgaa gttgtcccag tttgcctgtt ctacaccgaa
     1321 aaagctgtaa tgttgcactt tgttgtcaag cagcatcggt tgtttgaccg cttcggatat
     1381 ggcagataaa gcactggatt tttgttgtaa atattgcacg ccccagctgc ctttcagacg
     1441 gcctatgggt tggtggcgca actcgatacg ggcgttgtgt gttttgttgt tgaagaagtt
     1501 ctctactgca tcgcctgctt tttcgtcgtg gtggtagtca ttgcggttca gatgtacgcg
     1561 cagggcttca aaaccgggca gaggctgctt ccattcggcg cggagttcgt agcgtttgtt
     1621 gcgcaggtct atccacggtt tgccgttgtg ggcatgggcg tgtgcattat cgtcgtcatg
     1681 aaagccgcag ctcaagcccg ggttgtcgta atcgatgtct tcttcggtca gcaggtgcgg
     1741 ataaagctgc aaatagcgtt tgttaatcaa actcttttgc cagatgatgt cggcgtggca
     1801 atcatcgtat tcgtggctgt gggcaggcag gccgtattgg tctcggcggt cgctatacgc
     1861 cacgccgata aagccttttt cgccaaccca agacagcccg atactgcccg tttgcgaatc
     1921 ggcgtggctg tcgggcaggc gtttcagatt gcggtaacgc ggtacggcgt aatcgcccga
     1981 tttgcggtac agcccttccg tgtgcaacac gaagtttttg cccagaccga cattgatgcc
     2041 gccggacgtg agtttttcca gattgccgct gctcaaacgc agccccagtt cgcccgatac
     2101 gccgttttca ggcatttttt cggggatttt gccatcggca acatcgacca aaccggccac
     2161 attgcccgaa ctatataaaa gcgtaaccgg cccgcgcagg atttcgacct gttgcgacaa
     2221 ggcggtatct accataatgg cgtgatcggg cgaaaagtcc gccatatcgc ctgtttcgcc
     2281 gtgatggttc aaaaccttaa tccgcctgcc cgtttgacca cgaatgacag gagcggacgc
     2341 gccgccgccg tattgcgaag cgtggatgcc cggaacgccg tccaaagcgt cgcccaagtt
     2401 gacggctttt tggcgcaagg tatcgccgga gatgattttg tcggaggcgg tcgaagtgtg
     2461 caacagcccc gaagtggcgt gcggacggct tttgccgacg acgctgatcg tttccaagcc
     2521 caccgattgc tcagttccat gcgcttgggc gaggaggggt gtgttgatta aaagaattga
     2581 taaaacaatg ggtttgagtg tggtttgtgc cattttagct tctcttcgca tttcaaaatt
     2641 ttgttattat ataacattac attctttata tcataagatt ttgagaacac tcagagggca
     2701 taggcaaaaa tttttcaaat gaaacggttg tgatatttaa tggcgttaat ttgtattaac
     2761 cctaatttta gggaagttaa tgatgtgaac acgaacgccg ctatatttgg ctatcggata
     2821 accaaaattt atgagggcat aaaatcggca cgttcccgaa cggtcgaaaa ggtggcaaaa
     2881 tggcgtactt gtcagaatgc gaggctctgc gccagattcc taagggtgca tcaggcacgc
     2941 taagatataa ggggatatgc tcaggggtat cggaaatgca gccaataata aataatattt
     3001 taaatcaata ggttaatcac aaaatgcttt tgtttatcga caattacgac agttttactt
     3061 acaacatcgt ccagtatttt acagaattgg ggcaggaagt tgtcgtgcgc cgtaacgatg
     3121 atattacatt ggaagaaatc gaagccttaa agccgcaata tcttgtaatc ggccccggac
     3181 cgtgttcccc taaagaggcg ggtatttcag tagaagccat gcgccatttt gccggcaggc
     3241 tgccgattat gggcgtgtgc ctcggacatc agacgatagg cgaagcgttc ggcggagatg
     3301 tgatacgggc aaaaaccttg atgcacggca aggtgtcgcc tgtatcccat tcgggtaagg
     3361 gtatgtttaa agatttgccc aatcctgtta cctgtacgcg ttatcacagc ctcgttatcg
     3421 ctcgggatac gatgcccgaa tgtttggaag taacggcttg gactgaggac ggcgagatta
     3481 tgggcgtgcg ccataaggaa tatgccgtcg agggcgtgca gttccacccc gaagccctct
     3541 tgaccgaaca cggacatgat atgttgaaca attttttaat cgaatttcaa aacttcaaac
     3601 cgcaaaaaat ctgacgtgat gccgtctgaa tcccttcaga cggcattttc gtccgaatat
     3661 tgaacggagg acaaaaaatg attacaccgc aacaggctat cgaacgatta atcagcaata
     3721 atgagttgtt ttacgatgaa atgaccgact tgatgcgcca aatgatgagc ggaaaaatgc
     3781 cgcccgaaca aattgcggcg attttgacag gattgcgtat caaggttgaa accgtttccg
     3841 aaattaccgc agctgcagcc gtcatgcgcg agtttgcgac aaaagtgccg ctggagaatg
     3901 cggaggggct ggttgatatc gtcggtacgg gcggggatgg tgccaaaacc ttcaatattt
     3961 cgacgacttc gatgtttgtt gccgcagcgg caggcgcgaa ggttgccaaa cacggaggcc
     4021 ggtcggtctc ttcctccagc ggtgcggctg acgtggtgga gcagatgggc gcaaacctca
     4081 acctgactcc cgaacaggtt gcccaaagta tcaggcagac cggcatcggg tttatgttcg
     4141 cgcccaatca ccacagtgcc atgcgccatg tcgcccctgt acgccgttcg ctcggtttcc
     4201 gaagtatttt caacatattg ggtccgttaa cgaatcctgc gggcgcgccg aaccagcttt
     4261 tgggcgtgtt ccacaccgat ttgtgcggca tcctttccag agtattgcaa cagctcggct
     4321 ccaagcacgt tttggttgtt tgcggggagg gcggtttgga tgaaattaca ctgacgggca
     4381 aaacacgcgt tgccgagctc aaagacggaa aaatcagcga atacgacatc cgcccagaag
     4441 atttcggtat cgaaacccgc cgcaatttgg atgaaatcaa agttgccaat actcaggaat
     4501 ctttgttgaa aatgaatgag gtgctggaag gaagagaagg ggctgcgcgc gatatcgtat
     4561 tgcttaatac cgccgccgcc ctatatgccg gaaatgtcgc tgcttcgctt tcagacggca
     4621 tatctgccgc acgggaagcc atcgattcag gcagggccaa atcgaaaaaa gaggagtttg
     4681 tcggttttca accacaacaa agatgccatt ttcttggaaa gatggagctt gggtgatgcc
     4741 gccatgatta tggaactttt gtggcaaaac ataagcactt cacgaagaga acttaccaaa
     4801 ctgtttttat ataaaaactt ggggctgtac tagataacca gaccaaattc ccattaacta
     4861 attgtcttaa aatctgaatt tgagattcta tttaaaatgc cattggcatt tctttaaatg
     4921 cagccccaaa tgctctttgg gaatgccgtt aaacttacgt aaatggctaa attcactaat
     4981 atcaagcaca tcataactac gaaaggtatc cgtgtataca atgccatcag gcttaacttt
     5041 ctttcggata attggcaaca atgtcgctga ttgcgcatta ggaacaacga cggtataaac
     5101 cttatatatt gcgtccctaa gaagggacga ttaacaaaaa ttaacgtcct ttactttcta
     5161 caagtaacag ggcttttttt tgcccgtttt tgaggattcg caccatggaa gataagcaag
     5221 ggatgacaaa ggcggttgcc ggcgtgatga cggacgcgcc gacggacggc aggaagccgg
     5281 caaccgcttc aaaccttccc cccttatcta acgggggggt acagaaaccg aaacggcagg
     5341 cagggttcag gaagtcttcg aatgttacga aacgtacata acggacggta aaggaaacct
     5401 gttaggcgtt cctcttcggc gcggtgtatc agattcggct ttcattgatc aaat
//
LOCUS       DAWFBO010000136         5420 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFBO010000136 DAWFBO010000000
VERSION     DAWFBO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.136"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..297)
                     /locus_tag="WGO87_001668"
     CDS             complement(<1..297)
                     /locus_tag="WGO87_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0393005.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="WGO87_001669"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="WGO87_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0393006.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSAEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1662..4877)
                     /gene="carB"
                     /locus_tag="WGO87_001670"
     CDS             complement(1662..4877)
                     /gene="carB"
                     /locus_tag="WGO87_001670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0393007.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLVKEEKAVSDGILSDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRS"
     gene            complement(4906..>5420)
                     /locus_tag="WGO87_001671"
     CDS             complement(4906..>5420)
                     /locus_tag="WGO87_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393008.1"
                     /translation="KPDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFI
                     NFFKINVQAGAAKIREIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIY
                     CYDVRRNGKDKAGKPNRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDA
                     EIIREVTNRQ"
BASE COUNT         1221 a         1386 c         1409 g         1404 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacagcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcttt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgttccaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcacaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc
      841 gatggtgctg catctgctgg cgccgctggc agcaaaattc aacgaacgct atccgcatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctgc
     1081 agaagagctt gccggtcacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 caataaaacg caccccttta atgctgttta ttacagcgat aaagccgtca acctccgctt
     1381 gcgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg ccataaaatt gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gtcggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgacaccgt
     1621 aaaaaaaggt ttcagacggc attttcaacc cgattcaggc atcagctgcg gtttttcaaa
     1681 cgcccgtgca gttcttgaac gctgtacacg cccaagtggt cgcggctttt cgcgccttcg
     1741 ctcatcgctt cgccgcccgc agtagtggtg tattgcggca cacgttgctg caatgcgctt
     1801 tggcggatga tgtggctgtc ggacacggat tgcggatcgc tggaaacggt attcacgacc
     1861 agtgcgattt cgccgttttt cagcgcgtcg ccgatgtgcg ggcggccttc ggggacttta
     1921 ttgatggttt gtacaatcag cccgtgttcg gtcaggtatt gcgccgtgcc gcgcgtggcg
     1981 cagatgccgt agcctaaggc ttggaagttt ttggcggttt taatgacgcg ttctttgtct
     2041 tcttcgcgca cggagaggaa gattttgccg gtcgggttga ggcgttcgcc cgcgccgagt
     2101 tgggctttgt agtaggcttc gccaaagctt gcgcccacgc ccatgacttc gccggtggag
     2161 cgcatttccg gtcccaaaat cgtatccacg cccgggaatt tgatgaatgg gaacacggct
     2221 tctttaacgg catagaaatc ggggacaact tctttttcca cgccttgttc tttcagggaa
     2281 atgcctgcca tacagcgtgc gccgactttg gcgagcggca cgccggtggc tttggagacg
     2341 aagggcacgg tacggctggc gcgcgggttc acttccaaca cgaacaccac gccgtcttgt
     2401 acggcaaact gcacgttcat cagtccgacc acgcccagcg cgtacgccat ggctttggtt
     2461 tggcggcgga tttcgtcttg gatttcttcg ctgagcgagt agggcggcag cgagcagccg
     2521 gagtcgccgg agtggatgcc cgcctgttcg acgtgctgca tgatgccgcc gataaccaca
     2581 tctttgccgt ctgaaacgca gtccacatcg acttcaatcg cgttgttgag gaagaaatcg
     2641 agcaacacgg ggctgtcttc ggaaacttgt acggcttcgc gcatatattt ttgcaactgc
     2701 tcggcagagt gaacaatctg catcgcgcgt ccgcccaaaa cataagacgg gcgcacgacc
     2761 agcggatagc cgatttcttc ggctttgacg agtgcttctt cttcgttgtg ggcgatgcgg
     2821 ttgggcggtt ggcgcagacc taagtcgttc aacacttttt ggaagcgttc gcggtcttcg
     2881 gcggcgtcga tgctgtcggc ggacgtgccg atgatgttta cgccgttttc aaccaacgcg
     2941 ttggcgagtt tcagcggggt ttgaccgccg taatgcacaa tcacgcccca cgggttttcg
     3001 gtgcggacga tttccaacac gtcttccaat gtcagcggct cgaaatagag gcggtcgctg
     3061 gtgtcgaagt cggtggacac ggtttcgggg ttgcagttga ccatgatggt ttcaaagccc
     3121 gattcgcgca gggcgagtgc ggcgtgaacg cagcagtagt caaactcgat gccctgaccg
     3181 atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg acgggattcg
     3241 cattcttctt cgtaagtgga gtaaagatag gcggtttcgg tggcgaactc ggcggcgcag
     3301 gtatcgacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc gcgaacttct
     3361 ttttcgctta cgttcaacaa ttgtgccaaa cgtttgtcgg agaagccttt gcgtttcaga
     3421 cggcgtaggg cggcataatc caaatcactc aaaatgccgt ctgaaaccgc tttttcttct
     3481 ttcaccaagt cttcgatttg cgccaagaac caagggtcga tggcgcagat ttcgtggatt
     3541 tcttccaaag taaagcctgc gcggaacgca tcggctacaa agagcatgcg ctcaggaccc
     3601 gggttggcca gttcgcggcg gatttccgct ttgtcttcgc tgcgagggtt gaagccgcac
     3661 aagccggttt ccaaaccacg cagggctttt tggaagcttt cctgaatggt acggcccatt
     3721 gccattactt cgcccacaga tttcatttgc gtggtcaggc ggtcgtctgc ggcagggaat
     3781 ttttcaaacg cgaaacgcgg gattttggtt accacatagt cgatggaagg ctcgaacgac
     3841 gcgggcgtgc ggccgccggt gatgtcgttg cgcaactcgt ccagcgtaaa gccgaccgcc
     3901 agcttcgccg ccaccttcgc aatcgggaaa cccgtcgcct tggaagccag cgcggacgag
     3961 cggctcacgc gcgggttcat ctcaatcaca atcatctcgc cgttttcagg gttcaccgca
     4021 aactgaacgt tcgagccgcc ggtatccaca ccgatttcgc gcaataccgc caagcttgcg
     4081 ttgcgcatga tttggtattc tttgtccgtc agcgtttgcg ccggcgcaac agtgatggag
     4141 tcgcccgtat gcacgcccat cgggtcgaag ttttcaattg agcaaataat gatgcagttg
     4201 tcggccttat cgcgcaccac ttccatctcg tactctttcc agccgagcac ggactgctca
     4261 atcagcagct catgcgtggg cgacgcatcg aaaccgcgtt cgcaaatcgc caaaaactcg
     4321 tctttattgt aggcaatgcc gccgcccgaa ccgcccatgg tgaaagaagg acgaatcagg
     4381 gtagggaagc cgacctgttc ttgcgccgcc aaagcttcgt tcatcgtgtg gcagacaaaa
     4441 gatttcgggc aggagaggcc gattttctcc atcgcctcct taaagcggcc gcggtcttct
     4501 gctttgtcga tggcgtcttc ggtcgcgccg atcagctcga cattgtattt cgccagcacg
     4561 ccgttgcgcg ccaaatccag cgcacagttc agcgcggtct gaccacccat cgtgggcaga
     4621 atcgcatcag gccgctcttt ggcgataatc ttctccaccg tctgccacat aatcggctcg
     4681 atgtaggtaa catccgccat ttcagggtcg gtcataatcg tggcggggtt ggaattcacc
     4741 agaatgactt tatagccttc ttcacgcaaa gccttgcagg cctgtgcgcc cgaatagtca
     4801 aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat ggattttagg
     4861 tcggtacgtt tgggcatggt gggtctttct tttgaagaat ttggattatt gacgattggt
     4921 aacttctcta attatttctg catctgtttt ttcatcgctt ggattatctg aaaagaaaaa
     4981 actgtaatta ctatcggctt taaatttttc gtatagctct gggcgcaaaa tttttgcttt
     5041 ttcactattt tccacgctcc tgcgattagg cttgccagcc ttatctttcc catttctacg
     5101 gacatcatag caataaatag tgttaggaga attaggttct aaacgagtag ctacaatctt
     5161 tttataaatt atgtctaaat cttttttagt ttgtgttgga atgaaatctg caaaaagatt
     5221 actaaaactc tgaaaaattt ctctgatttt cgcagcacca gcctgaacat taattttaaa
     5281 aaaattaata aaatcagtag cacttttaaa atgaacttca taaaaatcag catacgcaaa
     5341 tattgaattt tcactattca aattaaaaaa ctctaatttt gcttgtgcat aatcatctgg
     5401 tctaggttca tcaggtttat
//
LOCUS       DAWFBO010000137         5343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFBO010000137 DAWFBO010000000
VERSION     DAWFBO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.137"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            42..515
                     /locus_tag="WGO87_001672"
     CDS             42..515
                     /locus_tag="WGO87_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0393009.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            641..1744
                     /gene="mnmA"
                     /locus_tag="WGO87_001673"
     CDS             641..1744
                     /gene="mnmA"
                     /locus_tag="WGO87_001673"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0393010.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGGGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYEGDVCLGGGIIQSTDKPVIITR"
     gene            1816..3486
                     /locus_tag="WGO87_001674"
     CDS             1816..3486
                     /locus_tag="WGO87_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEZ0393011.1"
                     /translation="MEKIWLDSYEQGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            3598..5232
                     /locus_tag="WGO87_001675"
     CDS             3598..5232
                     /locus_tag="WGO87_001675"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0393012.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1406 a         1452 c         1365 g         1120 t
ORIGIN      
        1 attaagcaca ttccccgtct gatattataa ggagcagaaa gatgaaaaaa ttattggcgg
       61 ccgtgatgat ggcaggtttg gcaggcgcgg tttccgccgc cggagtccac gttgaggacg
      121 gctgggcgcg caccaccgtc gaaggtatga aaataggcgg cgcgttcatg aaaatccaca
      181 acgacgaagc caaacaagac tttttgctcg gcggaagcag ccccgttgcc gaccgcgtcg
      241 aagtgcatac ccacatcaac gacaacggcg tgatgcggat gcgcgaagtc aaaggcggcg
      301 tgcctttgga ggcgaaatcc gttaccgaac tcaagcccgg cagctatcac gtgatgttta
      361 tgggtttgaa aaaacaatta aaagagggcg acaagattcc cgttaccctg aaatttaaaa
      421 acgccaaagc gcaaaccgtc caactggaag tcaaaaccgc gccgatgccg gcaatgaacc
      481 acggtcatca ccacggcgaa gcgcatcagc actaatctgc tgaaaatgtt tgaaatgccg
      541 tctgaaaaag cccgggcttt cagacggcat ttttatgact gcctttaaaa tgtgttaaaa
      601 tccgccttta aaaaccgccg tttccaacca tattctacgt atgaatacga cagcaaaccc
      661 ttccaatatc atcgtcgggc tctccggcgg tgtcgattct tccgtaaccg ccgccctgct
      721 caagcagcag ggttatcaag tgcgcggtgt gttcatgcag aactgggaag acgacgacaa
      781 cgacgaatat tgcagcatca aacaggattc gtttgatgcc atcgccgttg ccgatattgt
      841 cggcatcgac atcgacatcg ttaatttcgc cgcgcaatat aaagacaaag tctttgctta
      901 ttttcttcag gaatacagtg cggggcgcac gccgaatccc gatgtgttgt gcaacgccga
      961 aatcaaattc aaatgctttt tggattacgc cgtagggcag ggcgcggata ccatcgccac
     1021 cggacattat gcgcgcaaag aagtccgcaa cggcgtgcat tacctgctca aaggtttgga
     1081 tcgaaacaaa gaccaaagct atttcctcta ccgcctcaag cctttccaac tcgagcgcgc
     1141 gatttttccg ttgggcggtt tggaaaaacc tgaagtccgc cgccttgccg ccgaatttaa
     1201 tttgccgacc gccgccaaaa aagacagcac gggcatctgc ttcatcggcg agcgtccgtt
     1261 ccgcgagttc ctgcagaaat acctgccgac cgataacggc aaaatggtta cgcccgaagg
     1321 gaaaaccgtc ggcgaacacg tcgggctgat gttttacaca ttgggtcagc gcaaaggttt
     1381 gggcatcggc ggcgggggcg aaccgtggtt tgttgcggct aaagatttga cgaaaaacga
     1441 actgattgtc gtgcaaggac acgaccatcc gctgctctat acccgcagcc tcgtcatgaa
     1501 tgatttgagt ttcacgctgc ccgagcgtcc gaaggcagga cgctatacct gcaaaacgcg
     1561 ttaccgtatg gcggacgcgc cttgcgaatt gcgctatttg gatgatgaaa ccgccgagct
     1621 ggtgtttgac gaaccgcaat gggcggtaac gcccggtcaa tccgccgtgc tgtatgaggg
     1681 cgatgtctgc ttgggcggcg gcatcatcca atcaactgac aagcctgtca tcatcacgcg
     1741 ataaaggtaa tgccgtctga aacggttttc agacggcata gttctgcttc atttcattct
     1801 aaaagaccga aaattatgga aaaaatctgg ttagacagtt acgaacaggg cgtgaatgcc
     1861 gaaatcgaca tcacgcaata cggttccgtc agcgatgtat tttgccaaag tgtcaagaaa
     1921 tttgccggcc aacctgcttt tcaaaacatg ggcaagacgt tgacttatgc cgaaaccggc
     1981 aaactggcga ccgatttcgc ctcttatctg caaaacgtcc ttaaattgcc tcgaggagag
     2041 cgcgtggcaa taatgttgcc caatgtactg caatacccgg ttgcgctttt cggcattttg
     2101 caggcaggtt tggtggcggt gaacaccaat ccgctctata ccccgcgcga gttggagcat
     2161 cagttgaaag acagcggtgc gaccgccatc atcgttttgg aaaatttcgc caacacgctg
     2221 gagctggtgc tgccgcgcac gcagatcaaa cacgtcatcg tcgcctccgt cggcgaaatg
     2281 ttcgggctgc ttaaaggttc gctgatcaat ttcatcatcc ggaaaatcaa gaaaatggtg
     2341 cccgaatacc gtattcggga aaccgtttcc tttcagacgg cattgaaaga gggggcgaag
     2401 cacgttttcc aacctgtcgc attaaaccgc gaagataccg cgctgttgca atacacgggc
     2461 ggcacgacag gtgttgccaa aggggcggtg ctgagtcacg gcaacatctg cgccaatatg
     2521 cttcaggcaa aagaatggat taaaaaccag ttgcgcgagg gcaaagaaac cgttatcgcc
     2581 gccctgccgc tgtatcatat ttttgcctta accgtgaatc tgatgatttt tgccaatgcc
     2641 ggctcgaaaa tcatcctgat taccaacccg cgcgatatga aaggctttat cggagaactg
     2701 aaaaaacagc gggttaatgt atttatcggc gtgaacacgc tgtttaacgc gatggttaac
     2761 cgtcctgatt ttgccgaagt cgatttttca ggattgcggc tgactttggg cggcggtatg
     2821 gcgacccaaa aagccgttgc cgaaaaatgg aaaaaaatca ccggcacgcc catcgtcgaa
     2881 gcctacggtc tgaccgaagc cagccccggc gtgtgctgca accccttaaa catcgaatca
     2941 tacagcggca gcatcggttt gcccgtctcg tccaccgaag tcgaactgcg cgacgcaaac
     3001 ggcaaagaag tccccgtcgg gcagccgggc gaattgtggg taaaaggccc tcaagtgatg
     3061 caaggctact ggaaccgccc cgaagaaacc gccaaagcca tagacgcgcg cggcttcttg
     3121 gaaactggcg atattgccgt catggacgaa aaaggctggt tgaagctggt cgatcgtaag
     3181 aaagacctcg tcgttgtttc cggattcaat gtttatccga acgaaatcga ggaagtcatc
     3241 gcgcatcacg gcaaagtgat ggaagtcgcc tgtatcggcg tacccgacga aaaaaccggc
     3301 gaggcactca aagtattcgt cgtcaaaaaa gacccgtcat tgaccaaaga agaacttatc
     3361 gccttttgcc gcaccgaatt aaccgcatat aaagttccga aaaatatcga attccgcgac
     3421 gagttgccca agtccaacgt cggcaaaatc ctgcgccgcg agttgcgcca aagtaccggg
     3481 aaataaagaa aagatgccgt ctgaaaacag ccgtccaccg ttcagacggc atccgtccgt
     3541 ttgccagaac cgcgcgcttc acgttaaaat cacgcattcc aatatgggta ttccatcatg
     3601 accaaattca ttttcgtaac cggcggcgtt gtctcctcac tgggtaaagg tatcgccgcc
     3661 gcttctattg ccgccatcct cgaatcgcgc ggcttgaacg ttaccatgct caagctcgat
     3721 ccttatatca acgtcgatcc cggcacgatg agcccgttcc aacacggcga agtgttcgta
     3781 accgacgacg gcgcggaaac cgacctcgac ttgggacact acgaacggtt tatcgattcc
     3841 acgatgaccc gccgcaacag cttcagcacg ggacaggtgt acgaaaacgt tatcgccaaa
     3901 gaacgacggg gcgactattt gggcggcacg gttcaagtca ttccgcacat taccgacgaa
     3961 atcaaacgac gcatccatga aggcgcggca ggttacgatg tggcgattgt cgaaatcggc
     4021 ggcacggtcg gcgacatcga atcgctgccg tttttggaag ccatccgcca gatgcgaagc
     4081 cagttgggac gcaacaacac cctgttcgcc cacttgagct acgtccccta catcgccgct
     4141 gcaggcgaaa tcaaaaccaa gccgacccag cacaccgtta aagaaatgtt gagcatcggt
     4201 ttgcaacccg acatcctgat ttgccgtatg gacaggacaa tgcctgcgga cgaacgccgc
     4261 aaaatcgcct tgttctgcaa cgtggaagaa cgcgcgattg tcggcagcta cgatgtggac
     4321 agcatctacg aatgccccga aatgctgcac gaccaaggca tcgataacat cattaccgag
     4381 cagttgcagc ttaatgtgca gcaggcggat ttgaccgcgt ggaaaaaaat cgtccacgcc
     4441 atccaaaacc cgaaacacac cgtcaaaatc gccatggtcg gcaaatacgt cgatttgacc
     4501 gagtcctaca aatcgctgat cgaagccttg aaacacgcag gcattcacac cgaaaccgac
     4561 gtacaaatta cctttgttga cagcgaaagc atcgagaaaa acaacggcga cgtttctatg
     4621 ctcaaagata tggacgccat cctcgttccc ggcggttttg gttcgcgcgg cgtggaaggc
     4681 aaaatcgccg ctgtgcgcta tgcccgcgaa aacaacgtgc catacttggg catctgcctc
     4741 ggtatgcaga ttgcgctgat cgaatacgcc cgcgacgtgg caggtttgaa aggtgcgaat
     4801 tccaccgagt tcgacctcaa atgcgccgcc cccgtcgttg ccctgattga cgaatggcaa
     4861 accgccgacg gcagcgtcga aacccgcgac gaatccgccg atttgggcgg caccatgcgt
     4921 ttgggcgcgc aagaagtcga attgaaagca ggcagcctcg ccgccaaaat ctacggcagc
     4981 ggacacatcc gcgaacgcca ccgccaccgc tacgaagtca acaacaacta tgttcctcag
     5041 ttggaaaaag caggcttggt tatcggcggc gtatccgccg gacgcgaacg cttggtcgaa
     5101 accatcgaac tgccgaacca tccttggttc ttcgcctgcc agttccaccc cgagtttacc
     5161 tccaatccgc gcaaagggca tcctttgttc accgcgtttg tcaaagctgc gttgaacaat
     5221 aaaaaagcct gataaagcgt tacttgatga tcaaaatgcc gtctgaaagc ctgacaaaga
     5281 ccttcagacg gcattttcgc aagtcgggga ttataaaagc catcaattta atttttatgg
     5341 ttt
//
LOCUS       DAWFBO010000138         5335 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFBO010000138 DAWFBO010000000
VERSION     DAWFBO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.138"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            295..1728
                     /locus_tag="WGO87_001676"
     CDS             295..1728
                     /locus_tag="WGO87_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0393013.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGETLAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLIKENKEKLARIMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALVVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALLKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1837..2625
                     /locus_tag="WGO87_001677"
     CDS             1837..2625
                     /locus_tag="WGO87_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0393014.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLEVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            2993..4549
                     /locus_tag="WGO87_001678"
     CDS             2993..4549
                     /locus_tag="WGO87_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0393015.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPAVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4566..5312
                     /gene="surE"
                     /locus_tag="WGO87_001679"
     CDS             4566..5312
                     /gene="surE"
                     /locus_tag="WGO87_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0393016.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
BASE COUNT         1246 a         1341 c         1525 g         1223 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgtt ccttatatat
       61 tattaaaggg ctgcattact tttctttcta aattaccatt gtataaaaag tatttttctt
      121 aaacgacagg gattgcgccc gcgccatatt cagacaccgc ccggatgttg cgctgcccga
      181 tcggatgctt cagacggcat cggaagggtt tgcagttttg gaatatgaag atgataatgt
      241 ccgcgagatt gacggcattt gaaagcggtt attccgcata ccggaggata cgaaatgaac
      301 gaatattacc aattaatcaa gcatcccgat atttcccttt ccccgatttc agacggcatc
      361 ggggtcggca atccggcgac gggcgagact ttggcgtatg tccgcaatac gggttcggac
      421 aaactgaaaa acctgattca aaaagcagct gcggcacaaa aattatgggc ggcaaaaacc
      481 gctttggaac gcgccgacat attgtggcgt tggtattttt tgattaaaga aaacaaagaa
      541 aaactggcgc ggatcatgac gatggagcag ggtaaaagcc tgactgagtc gcgtggtgaa
      601 atcgattatg cggcttcgtt tgtgcgctgg tttgccgaag aagcgcggcg gattgacggc
      661 gatgtgctga cgagtgtgaa agcgtcgcaa aaactggttg tgttgaaaca gcctgtcggc
      721 gttaccgctg cgattacgcc gtggaacttc ccatctgcaa tgattgcgcg caaggctgcg
      781 cctgctttgg cggtgggctg cgcgatgatc gtcaaacccg catcgctcac gcctttaagc
      841 gcgtatgcgt tggtcgtgct ggcttatgaa gcgggcgtgc cgcaggattt gctgcccgtc
      901 gtcagcggca gtgcatcgga aatcggccgt gaatttgcca cgaacccgat tatccgcaaa
      961 atcagcttca ccggctctac cgaagtcggt gcgaaaattt ttgccgacag cgcggcggac
     1021 attaaaaaac tcagtttgga gctgggcggc aacgcgccgt ttatcgtgtt tgacgatgcc
     1081 gatttggaca aagccgtcga aggtgcgctc gccagcaagt tccgcaacag cggtcagacc
     1141 tgcgtctgca ccaaccgcgt ttacgcccaa tcgggcattt acgacgcatt ttgccgcaaa
     1201 ttgagtgaaa aagcagccgc gctcaaattg ggcaacggct tggaggatgg cgtaaaccaa
     1261 gggccgctga ttgaggaaaa agcggtggag aaagttgagc agcacatcgc cgacgcgctc
     1321 ttaaaaggcg cggtctgtct gaccggcggc aaacgcagcg cgttgggcgg aacgtttttt
     1381 gagccgaccg tcttaagcgg cgtaacggcg caaatggcgg tagctcgcga ggaaaccttc
     1441 gggcctttgt gtccggtatt ccgttttgaa accgaagccg aggtcatcga ggctgcgaac
     1501 aatacggaat acggtttggc agcttacctt ttcaccgccg acaccgcccg ccaatggcgc
     1561 gtcggcgaag ccttggaata cggcatggtt ggcatcaata cgggcttaat cagtaatgaa
     1621 gcggcaccgt ttggcggcgt gaaacgcagc ggtttgggac gtgaaggcag caaatacggt
     1681 gcggacgaat atctggaatt gaaatatctg tgtatggatg tcgggtgacg gatgccgtct
     1741 gaacggcagg gtttcagacg gcgccgcatt ttaagcagtc tctatctgtt gtacaatgcg
     1801 ccctgttttt acggttattt ttgatttgaa caagatatga tggaaaacgg aaaaacagtc
     1861 ccgagatggc gttttgcctt gaaaagtgcg ggctggcatc tcttaatcag cctgtcggtt
     1921 gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta tgccgagctg
     1981 acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg tggtccgctg
     2041 ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt cgatttttcc
     2101 atggtcggca tcatccagct ggcggctttg gtgtacggtc tgcacagcgt ttctttggcg
     2161 cgtcccgtgg tggaggcgtt tgaaaaagac aggattaatg ttgtgaccgc cgccgaagtg
     2221 gcggttgaag acctgcacaa agcgccggaa gggctgcaaa gcctgtcgtg gttcggcatc
     2281 cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac gctggatttg
     2341 tccctgaaag gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta ttccgacaag
     2401 gaagcagaag aaatccgcaa acatctgaaa ccgctggaag tcttggcgga tgcgagaaaa
     2461 acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga gctgtattac
     2521 ctgccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa agagggcaac
     2581 accaaaggtt acgcgccgat agacggcttc atcattaccc cttaagcgtt gggactccgt
     2641 ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga gaggggcgca
     2701 acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg tgttgaaaca
     2761 tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcgt tttgccattg
     2821 ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc acatgcaaga
     2881 ccccgagcag agcagcaagc ccgcccgccg ttttttgtac gtaccgtctg ttcaggcagc
     2941 ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa tcatggattt
     3001 tagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg tgttggaagt
     3061 cgtattgggg atagacaatc ttgtctttgt ggcgattttg gcaaacaagg tccagcccgc
     3121 acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc gcatcattat
     3181 gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa tcggcggcct
     3241 cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc tttacaaagc
     3301 caccaccgaa ctgcatgaac gcctcgaagg gcacaaccgt tttaccgttg ccgacagcca
     3361 aaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg atgccgtgtt
     3421 ttccatcgat tcggtcatta ctgcggtggc gatggtcgat catatcgtcg tggcgatggg
     3481 tgcggtcgtc gtcgcaatgg ctgtaatgat ttctgccagc aaactcttga ccgaatttgt
     3541 ggacaggcat cccgccgtcg tgatgctctg ccttggtttt ttgttgatga tcggtttcag
     3601 cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg ccgccatcgg
     3661 cttctcgatt ttaatcgaat tgtttaacca gatttcgcag cgcaacagcc gcaaaaacga
     3721 ctacatcggc agctcgtggc gcaagcgcac cgccgaaaac gtcttgggta tgatgggcat
     3781 acgcgaaagc gtgcttgccg atgcgggcgg cgaatccggg gacgatgcgc attttgaaga
     3841 aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga ttatgggggt
     3901 gatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg aagaacagtg
     3961 tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggtaagg cgggcgtgga
     4021 cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg aaacaggcgg
     4081 tctcgacatt cagacggcat tgcgccagcc gctcgttttg cccgacagca ccaccgcgct
     4141 gggcgcaatc gaactcttcc gccaaagcag cgcggattac gccctcgtcg tggacgagtt
     4201 cggcgcggta ttgggcatgg taaccatgaa agacctgctc gaaaccatcg caggcgagtt
     4261 ccccgaagaa tttgagcgcg aagaagaacc agccgttcag gggaatcccg atgaaagcct
     4321 gacggtggaa ggcgcgttgg aatatgtgga actcgcaccg caactcaacc tgccgcagca
     4381 ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat tgcaaaccat
     4441 ccccgatgtc ggtgattttg ccgatttcca cggctggcgg tttgaagtgg tcgaaaaaga
     4501 agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag cattcaggat
     4561 agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggc atcgccgttt
     4621 tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt tgcgcccgaa cgcgacagga
     4681 gcggggtcag caattcgctg acactggaac gccctttgca gttgaaacag gcgcaaaacg
     4741 gcttttacta tgtcaacggc acgccgaccg actgcatcca catcgggcag tctgtatttt
     4801 cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat atgggggacg
     4861 acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg ggcatgcccg
     4921 ctgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcaaccgca gaaaaggcac
     4981 tgtggacatt gttggcgcat tttttcaaaa accccccgca gtcccctatt ttatggaaca
     5041 tcaatatccc cgccgtcgcg ccggaagatg tgcggggcat taaaatcgcc cgtttgggca
     5101 ggagacacca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa cagatttatt
     5161 ggatagggcc ggtcggcgaa gtttccgatc gggaagaggg aacggatttc ggtgaatgcg
     5221 gcgcaggttt cattaccgta acgccgttgc aaatcgacct gaccgcctat ccggacatgg
     5281 cggaaacggc ggcgttctgg catacggact gaccgtttca tcaaatatag tggat
//
LOCUS       DAWFBO010000139         5310 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFBO010000139 DAWFBO010000000
VERSION     DAWFBO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.139"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..251)
                     /locus_tag="WGO87_001680"
     CDS             complement(<1..251)
                     /locus_tag="WGO87_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
                     /protein_id="HEZ0393017.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRRGTETET
                     AGRVQEVFECYEMYITDGKGNLLGVPLRRGVSDSAFIDQ"
     gene            complement(323..568)
                     /locus_tag="WGO87_001681"
     CDS             complement(323..568)
                     /locus_tag="WGO87_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393018.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(569..865)
                     /locus_tag="WGO87_001682"
     CDS             complement(569..865)
                     /locus_tag="WGO87_001682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393019.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHLARYAVTFENNVDSKLRRH"
     gene            complement(856..1209)
                     /locus_tag="WGO87_001683"
     CDS             complement(856..1209)
                     /locus_tag="WGO87_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393020.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1445..2872
                     /locus_tag="WGO87_001684"
     CDS             1445..2872
                     /locus_tag="WGO87_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0393021.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            2941..4344
                     /locus_tag="WGO87_001685"
     CDS             2941..4344
                     /locus_tag="WGO87_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0393022.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4508..>5310)
                     /locus_tag="WGO87_001686"
     CDS             complement(join(4508..5040,5040..>5310))
                     /locus_tag="WGO87_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0393023.1"
                     /translation="EQCKNISQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGLNAVK
                     SDRQKPAQYIGQHQDAYLHEIAKHFDCTAATVCYALKQMGITRKKRPTTYKEQDPAKV
                     THYLTQPAEFSDYQRVYLDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQ
                     VGNRLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKL
                     GHKVLPLAPYSPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1334 a         1285 c         1370 g         1321 t
ORIGIN      
        1 atttggtcga tgaaagccga atcggataca ccgcgcctaa gaggaacgcc taacaggttt
       61 cctttaccgt ccgttatgta catttcgtaa cattcgaaga cttcctgaac cctgcctgcc
      121 gtttcggttt ctgtacccct cctgttagat aaggggggaa gatttgaagc ggttgccggc
      181 ttcctgccgt ccgctaacgc gtccgtcatc acgccgacaa ccgcctttgt catcccttgc
      241 ttatcttcca tggtgcgaat cctcaaaaac aggcaaaaaa aaagccctgt tacttgtaga
      301 aagtaaagga cgttaatttt tgttaatcgt cccttcttag ggacgcaata tataagggac
      361 gcaatatata aggttttaaa acgctaagaa caaaatccga taacggctct gaagaagtaa
      421 gtctttcttt attgcacaag atagcaaact tccacggcat tctcgcccca cccgtgccgt
      481 ccaagccttt ttcaaagaca tagatggttt tcttggcaat cacttcgtca gtttggcggt
      541 cgataatccg tatggtcgca cctgccactc agtgcctgcg cagcttggaa tcgacattgt
      601 tttcgaacgt taccgcataa cgggcgaggt gtatatgatt aagtatttga tttatcggaa
      661 aatctttacc tgaataccga ataatatcgg aatggttggg ttgcaggaca tccacatagc
      721 ggtattcccc atcgctaaaa aatcctagga aacgagcaat aaaatttact ccttcgctgg
      781 tctgtaaagc cgcattgtcc cacatcgggt ctctggtttt tgcatctgcc gaaacggtac
      841 gctcaggtac taccttcaac agcataatcc cttccacatt gtccgccgtc tggtaaatct
      901 ttcccccgcc gttttgcatt gttcgttaaa cacggcttcg gcttctttgt attttctgtc
      961 ccactcttct tgtgcctgta tttcttcttt gatcgggccg aattgtttgg gaataatcca
     1021 aacaaacagc atcaggatag cggcggcggt caggctgcct gaaaggattt tgccggggtt
     1081 ccgtttgggc tttttatagg caaagcggac gagaaaccaa agcaacagca gcatggtgcc
     1141 ccaatagccg attgagaata ggatggccaa accttctagg aaatggcgta aatcgtttgt
     1201 ggtaaacatg ggttgttcct gtggttaaat gtgcaggctg ctttttgccg aatcttgccg
     1261 catctcaaaa gcagcctgcg cttcagcgtt gcgttacgca gtaaaataat gaatatttgt
     1321 aacgacttgg gtattttttg tcaatattcc cgcctttccc ttaacagctg ccgcgctttc
     1381 cgttaaaatt cctttacata tttatattgt ttcctgtttc tatattgcca aggttatacc
     1441 cgttatgttt ttctccgccc tgaaatcctt tctttctcga tacattactg tatggcgcaa
     1501 tgtttgggcg gtgcgcgacc agttggaacc gcccaaacgc acggcggaag aacaggcgtt
     1561 tttgcccgcg catttggaac tgaccgatac gccggtctct gccgctccga aatgggcggc
     1621 gcgttttatt atggcgtttg cgcttttggc tttgttgtgg tcctggttcg gcaaaatcga
     1681 tattgtggcg gcggcttcgg gcaaaacggt gtcgggcggg cgcagcaaaa ccatccagcc
     1741 gctggaaacg gcggtggtta aggcggtaca tgtgcgcgac gggcagcatg tgaaacaggg
     1801 agaaacgctg gcggaactgg aggctgtggg aacagacagc gatgtggtgc agtcggagca
     1861 ggctttgcag gctgcccaat tgtccaaact gcgttatgaa gcggtattgg cggcattgga
     1921 aagccgtacc gtgccgcata tcgatatggc gcaagcacgg tctttaggtc tctccgatgc
     1981 cgatgtgcaa tcggcgcagg tgttggcgca gcaccagtat caggcatggg cggcgcagga
     2041 tgcgcaattg cagtcggctt tgcgcggcca tcaggcggaa ttgcagtcgg ccaaggcgca
     2101 ggagcagaag ctggtttcgg tgggggcgat cgagcagcag aaaacagcag actaccgccg
     2161 tttgcgggcc gacaatttta tttcggaaca tgcgtttttg gagcagcaga gcaaatcggt
     2221 cagcaattgg aacgatttgg aaagtacgcg cggtcagatg aggcagattc aggcggccat
     2281 tgcacaggcg gagcagagtc gggtgctgaa tacgcagaac ctgaaacgcg atacgctgga
     2341 tgcgctgcgc caggcaaacg aacagattga ccaataccgc ggccaaacgg ataaggcaaa
     2401 gcagcggcag cagctgatga caatacagtc gcctgcggac ggcacggtgc aggaattggc
     2461 cacctatacg gtgggcggtg tggtgcaggc tgcccaaaaa atgatggtgg ttgcgcccga
     2521 tgacgacaaa atggacgtgg aagttttggt attgaacaaa gacatcggtt ttgtggaaca
     2581 gggacaggat gcggtggtga agattgagag ttttccctat acgcgctacg gttatctgac
     2641 gggcaaggtg aaaagtgtca gccatgatgc ggtaagccac gaacagttgg gcttggttta
     2701 tacggcggtg gtgtcgctgg acaaacatac cttgaatatt gacggcaaag cagtgaatct
     2761 gacggcgggc atgaatgtca cggcggagat taaaacgggt aaacggcggg tgctggatta
     2821 tctgttaagc ccgctgcaaa ccaaattgga cgaaagcttt agggagcgat aggcggatcc
     2881 gtactgggca tttgttatcc gccggttcgg acatgcagac tgcctgaaac cattgcccgg
     2941 atgacattgc tcaatctaat gataatgcaa gattacggta tttccgtttg cctgacactg
     3001 acgccctatt tgcaacatga actattttcg gctatgaaat cctatttttc caaatatatc
     3061 ctacccgttt cactttttac cttgccacta tccctttccc catccgtttc ggcttttacg
     3121 ctgcctgaag catggcgggc ggcgcagcaa cattcggctg attttcaagc gtcccattac
     3181 cagcgtgatg cagtgcgcgc acggcaacaa caagccaagg ccgcattcct tccccatgta
     3241 tccgccaatg ccagctacca gcgccagccg ccatcgattt cttccacccg cgaaacacag
     3301 ggatggagcg tgcaggtggg acaaacctta tttgacgctg ccaaatttgc acaataccgc
     3361 caaagcaggt tcgatacgca ggctgcagaa cagcgtttcg atgcggcacg cgaagaattg
     3421 ctgttgaaag ttgccgaaag ttatttcaac gttttactca gccgagacac cgttgccgcc
     3481 catgcggcgg aaaaagaggc ttatgcccag caggtaaggc aggcgcaggc tttattcaat
     3541 aaaggtgctg ccaccgcgct ggatattcac gaagccaaag ccggttacga caatgccctg
     3601 gcccaagaaa tcgccgtatt ggctgagaaa caaacctatg aaaaccagtt gaacgactac
     3661 accgacctgg atagcaaaca aatcgaggcc atagataccg ccaacctgtt ggcacgctat
     3721 ctgcccaagc tggaacgtta cagtctggat gaatggcagc gcattgcctt atccaacaat
     3781 catgaatacc ggatgcagca gcttgccctg caaagcagcg gacaggcgct tcgggcagca
     3841 cagaacagcc gctatcccac cgtttctgcc catgtcggct atcagaataa cctctacact
     3901 tcatctgcgc agaataatga ctaccactat cggggcaaag ggatgagcgt cggcgtacag
     3961 ttgaatttgc cgctttatac cggcggagaa ttgtcgggca aaatccatga agccgaagcg
     4021 caatacgggg ccgccgaagc acagctgacc gcaaccgagc ggcacatcaa actcgccgta
     4081 cgccaggctt ataccgaaag cggtgcggcg cgttaccaaa tcatggcgca agaacgggtt
     4141 ttggaaagca gccgtttgaa actgaaatcg accgaaaccg gccaacaata cggcatccgc
     4201 aaccggctgg aagtaatacg ggcgcggcag gaagtcgccc aagcagaaca gaaactggct
     4261 caagcacggt ataaattcat gctggcttat ttgcgcttgg tgaaagagag cgggttaggg
     4321 ttggaaacgg tatttgcgga atagagcagg ctgaaacggt tatgaaattc ccaaagcagc
     4381 ctgcaccccg tttcgaaagt gcaggctgct ttgggattga tccgatactt tcacattctc
     4441 attgccttta aataaagcac agttaaaaac acatttaaat taagtgcgaa ttaattcaaa
     4501 tgtatattca attaaaatca aaataggaca gtagtgcatc gtcaaatcgg gcgtaatcag
     4561 acaaaacggt tcgcagatac cgcttaatat tcgcccacac tttctcaatc gggttgagct
     4621 caggtgaata aggtgcaaga ggcaatacct tatgtcccaa tttttccgcc atttcccgta
     4681 agacacccat acggtgaaat cgcgcattat ctaaaataat caccgatttt tgagtcaatg
     4741 cgggcagtag gcattgctga aaccacgctt caaaaaagac tccggtcatc gtattttgat
     4801 aaaccatcgg agcaatcagc cggttgccga cttgtgcgga caccaaagat aagcgttggt
     4861 atctttttcc acttatctgc gctttcacta tttgcccttt caggctgcgg gcatagggac
     4921 ggaacaggta gcggtcaaat cctgtttcat ccaaataaac acgttggtag tcggaaaatt
     4981 cggccggctg tgtcaaataa tgcgttactt tggccgggtc ttgttctttg taagtggtgg
     5041 tctttttttg cgcgttatcc ccatctgttt gagtgcatag caaacggtgg ctgccgtaca
     5101 atcaaaatgt ttggcgattt catgcagata ggcatcctgg tgttgcccaa tatattgagc
     5161 cggtttttgc ctatccgatt tgacggcatt tagaccggta acttgatgtt ttaggctgcc
     5221 tgtttgtttt ttaaggcgaa tccacaggta aagcgtgttt cttgacaagt taaacgttgc
     5281 tgcggtttgg ctgatgtttt tgcattgttc
//
LOCUS       DAWFBO010000140         5266 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFBO010000140 DAWFBO010000000
VERSION     DAWFBO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.140"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            109..804
                     /gene="pgl"
                     /locus_tag="WGO87_001687"
     CDS             109..804
                     /gene="pgl"
                     /locus_tag="WGO87_001687"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0393024.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            785..1771
                     /locus_tag="WGO87_001688"
     CDS             785..1771
                     /locus_tag="WGO87_001688"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0393025.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1821..2669
                     /gene="hexR"
                     /locus_tag="WGO87_001689"
     CDS             1821..2669
                     /gene="hexR"
                     /locus_tag="WGO87_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0393026.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2790..4436
                     /gene="pgi"
                     /locus_tag="WGO87_001690"
     CDS             2790..4436
                     /gene="pgi"
                     /locus_tag="WGO87_001690"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0393027.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            4450..5001
                     /locus_tag="WGO87_001691"
     CDS             4450..5001
                     /locus_tag="WGO87_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0393028.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAKGETPNGIKVSELACKTVETEARLEELY
                     NRKKALIDEMVREADKKELPKRL"
     gene            5046..5186
                     /locus_tag="WGO87_001692"
     CDS             5046..5186
                     /locus_tag="WGO87_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393029.1"
                     /translation="MPGYRNWQNGLNAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
BASE COUNT         1335 a         1467 c         1397 g         1067 t
ORIGIN      
        1 cagtcccgat atttggtatc aaaagcataa acaactgttc ggccggcata ttgctcaacc
       61 catttccata acggaatatg ccgtctgaaa taaaaaaagg acacaaatat gtttgtttgg
      121 cacgaatacg aaaatgcggc agaagcggcg cagtctttgg ctgacgcagt ggcggatgct
      181 ttgcagggcg cactggacga gaagggcggt gcggtgttgg cagtttccgg cggacgttcg
      241 ccgattgcat ttttcaacgc cctgtcgcaa aaagatttgg attggaaaaa cgtcggcatc
      301 accttggcag atgaacgcat cgtgccgacc gtccacgccg acagcaatac cggtttggtg
      361 cgcgaatacc tgttgaagaa caaagcggaa gcggcaatgt ggattcctat ggtggaagac
      421 ggaaaaactg aaaccgaatt acatcccgat gctgttgtcg attatgcact gaaacattac
      481 aaacagcccg atgttttggt tttgggtatg ggaaacgacg ggcatacggc ttcgattttc
      541 ccgaaagctc cgcagtttca gacggcaatc gacggttcgg caggtgtcgc gctggtgcat
      601 accacgcccg ttaccgcgcc gcacgagcgc gtcagtatga ccttggatgc gattgcccat
      661 acggggcatg tgtttttggc gatacggggc gaagagaaaa aagccgtgtt cgaccaagcc
      721 gcacaaggcg aaaaccgcga atatccgatc aacctcgttt tgaaccatca aggagtgaac
      781 tgccatgtct tctacgccga ataaacaagc cggatatccc cgactggtcg ccgatatcgg
      841 cgggacgaat gcacgctttg cgctggaaac cgcgccgcgc gtcattgaaa aagccgccgt
      901 gcttccgtgt aaagactacg atacggttac cgatgcggtg cgtgcctatc tgaatcaaag
      961 cggtgcaaca gccgtacggc acgcggcatt tgccatcgcc aacccgattt tgggcgactg
     1021 ggtgcagatg accaaccacc attgggcgtt ttccatcgaa accacccgtc agactttggg
     1081 gctggacacc ctcatccttt tgaacgactt taccgcgcag gcattggcgg taacgcagac
     1141 ttcaagcaaa gacctgatgc aggtaggcgg gcaaaagcct gtcgaatttg cccccaaagc
     1201 cgttatcggc cccggtaccg gcctgggcgt gagcggattg gtgcacagcc acgcaggctg
     1261 ggtggctttg gcaggcgagg gcgggcatac cagtttcccg ccgtttgacg atatggaagt
     1321 gctgatttgg cagtacgcca aaaacaaata cggccatgtt tccgccgaac gctttttgag
     1381 cggcgcgggc ttgagcttgg tttacgaggc tttggctgca aaacagaaag ccaaacccgc
     1441 caaactgatg ccgtctgaaa tcacggaaaa ggctttgagc ggcgcgtcgc ctttgtgccg
     1501 tcagactttg gacatcttct gcgccatgct cggcacggtt gcttccaacc tcgccctgac
     1561 gctgggtgcg cgcggcggcg tgtacctgtg tggcggcatt attccccgcg tgttggaata
     1621 tttcaaaact tccccgttcc gcagccgttt cgagaacaag ggcaggtttg aagcatatct
     1681 tgccgcgatt cccgtgtatg tcgtcttgag cgagtttccc ggaatttccg gtgcggctgc
     1741 ggctctcgac aaccatttga gaaacgttta accacagcgg ctccttgcag cggggctgca
     1801 ttatcgaagg gcatatcatt atgttaagca aaatcagcga atcactggca aacctttccg
     1861 gtgcggaacg caaagtcgcc gaatgtgcat tggcggaacc caaatggttc gtccatgccg
     1921 ccgttgccga aattgccgaa cgtgcgtccg tcagccagcc gaccgtcatc cgattctgcc
     1981 gcagcttggg ttataaaggg ctgcccgagt tcaagctcgc cttgtccgcc agcatcggtc
     2041 atgagggtat gccctatgtc cacgaagaac tcaacgccga cgacgatatg gcaagcgtgg
     2101 tcgagaaagt attgggcaat gccgccgcct cgctgttggg cgaacgccgc ttcctgaaag
     2161 agtcggagct ggaaaacgcc attgccacgc tgatgcacgc ccgccgcgtc gagttttacg
     2221 gtgtcggcaa ttccggtatc gtcgcgcagg acgcgcagca caagtttttc cgtttcggaa
     2281 tgtccacggt cgcctatgtc gatacgcaca cgcagctgat ggcggcatcc gttttgagcg
     2341 atcaggatgt tttggttgcc atttccaaca cgggttcgtc tatcgaactt ttggatgcgg
     2401 tcagcatcgc caaagaaaac ggcgcgtccg tcatcgcact gacccgcaac gattcgcctc
     2461 tggcgcaact tgccgactgc gtgttgagcg ttgccacaca ggaaaatgcc gaactctaca
     2521 cgcccatggt ttcccgcctc ttgcagcttg ccgtcatcga catccttgcc atcggacttg
     2581 ccctgcgctt gggcgatgct gccagcctgc aattgcagaa aagcaaaaaa agcatacaca
     2641 acaagcacat cgattacgac aaagattgac cttcagacgg catcccacaa atgccgtctg
     2701 aaatgccgaa caacggtcgt cggcggcttg cggcagtttc cggcagcctt ttcatcccac
     2761 aacaaaaaac ctcattcagg agcatataga tgaaacacct tcacgactta cccgcatggt
     2821 cgaaattgtg gaaccacttt gacgacagca aaacattgca tatgcgcgaa atgttcgagc
     2881 aagacccgca gcgtgcggaa cgctactggc tgcaagtcgg cggactgacg ctggactact
     2941 ccaaaaaccg catcaacgac gaaaccatgt cgcttttgtt cgagcttgcc cgagaagcag
     3001 gcgtgccgga gcggatgcgg cagatgttcc acggcgaaaa aatcaatacc accgaaaacc
     3061 gcgccgtcct gcatgtcgcc cttcgcaacc gcaccaattc gccgattgtg gttgacggtg
     3121 aagatgtgat gcccaaagtc aaccgcgttt tgcaacgtat gggcgaattt gcacacgaag
     3181 tccgcagcgg aagctggctg ggctatacca accaagtcat taccgacgtt gtcaacatcg
     3241 gcatcggcgg atcggatttg ggtccgctga tgatgtgtac cgcgctcaaa cctttcggtc
     3301 atccgcgcct caatatgcac ttcgtctcca acgtggacgg ctcgcaactg cgcgacgtat
     3361 tgtccaaagt ccaccccgaa accacgctgt tcatcatcgc ctccaaaaca tttaccacgc
     3421 aggaaacgct gaccaacgcg ctgaccgcgc gcgaatggtt tttgaatcat gcgggcgacg
     3481 aagaagccgt tgccaaacac ttcgccgccg tttccaccaa tcaaaaagcc gtcgccgaat
     3541 tcggcatcga caccgccaat atgtttgaat tttgggattg ggtcggcggt cggtacagcc
     3601 tgtggtccgc catcggattg ccgattatgc tgtatctcgg cgaagaaaac ttcatcgaaa
     3661 tgctcaacgg cgcgcacctg atggaccaac acttcatcaa cacaccgctc gagcgcaacc
     3721 tgcccgtcat tctcgccctc atcggcatct ggtatatcaa ctactacggc ggcggcagcc
     3781 acgtcatcgc gccttacgac caacatttgc accgcctgcc caaattcatc cagcagctcg
     3841 atatggaaag taacggcaaa caggttacgt tggacggcaa agcagtcgga cacgaaacct
     3901 cgccgattat ctggggcgaa acgggcatta acggccagca cgccttcttc caactgctgc
     3961 accaaggcac gcacattacc cccatcgacc tgattgcctc gcttgaaaaa cgcagcaacc
     4021 tgcccggaca ccacgaaatc ctgcttgcca acgtcttcgc ccaagcagaa gcctttatgc
     4081 gcggcaaaac ccccgacgaa gtccgcgccg aactcaaagc gcagggtatg gatgaggcgc
     4141 gcatcgaaga gctggtcccg cacaaaacct tctccggtaa ccgcccgacc aacctcatcc
     4201 tgatggacaa ggtcaacccg cgtaatatgg gcagcctgat tgccatgtac gaacacaaaa
     4261 ccttcgtaca aggcatcatt tggggcatca acagcttcga ccagtggggc gtggaactcg
     4321 gcaaacaact ggctaaaacc attttgggcg aactgaccgg cgaaaccggg tcgcaaaagc
     4381 acgacagctc gaccgaacgc ctgatcaacc tctacctgca gaccaaccgc aaataaaacc
     4441 tgcggaaaaa tgccgtctga acgccgaccg tttcaaacgg catttttatc gaacaggaaa
     4501 accgtcggta aacttgcaga gcgtgtgcaa tcccgatatg atggtttgca taaatttaaa
     4561 catatatgtt ccgcagctat ggcactgatt aaagagccgt tggacaaagc gaaacaaagg
     4621 aacgaagaac ttgaagcggc agaagaagcg gcggcgcagg aggcattggg tcgggagcag
     4681 gaagccgccc gcgtatccga atgggaagaa cgctacaagt tgtcgcgcag cgagttcgag
     4741 cagttctgga aaggattgcc gcaaactgta cagaataaac tgcaagcttc gcagaaaaca
     4801 tggaaaagcg gtatggacaa aatctgtgcc aacaatgcga aagccaaagg tgaaacgcca
     4861 aacggcataa aagtcagcga actggcatgc aaaacggtgg aaaccgaagc acgcttggaa
     4921 gagctgtaca accgtaaaaa agcccttatc gacgaaatgg tcagggaagc ggacaagaaa
     4981 gaactgccaa agcggctctg aacagcgcgg ttcaggcact gcccgccgat attgccgaaa
     5041 ccgtcatgcc cgggtaccgc aactggcaaa acggtctgaa tgccaaatgc gctgacggcg
     5101 acgaatacgg cgtggcacaa tccgactgcc gtaccagaga aatcaatgcg aaaaccaaag
     5161 aaatccaagg ttatctgatt gactgaaact tggatgcggg aatgtcggcg gcttttgcgt
     5221 ttttgtcgtt tttatagtga attaaattta aaccgataca tcgttg
//
LOCUS       DAWFBO010000141         5223 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFBO010000141 DAWFBO010000000
VERSION     DAWFBO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.141"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(103..2937)
                     /gene="lbpA"
                     /locus_tag="WGO87_001693"
                     /gene_synonym="tbpA"
     CDS             complement(103..2937)
                     /gene="lbpA"
                     /locus_tag="WGO87_001693"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0393030.1"
                     /translation="MNKKHGFQLTLTALAVATAFPAYAAQAGGAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIQSQDKTGQFFSVEGERES
                     KPVAKLAGYGKYLNNQLNRWVKERIEQNQPLSAEEEAQVREAQVRHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISEST
                     GFDEKNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPDSAGRLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2934..5066)
                     /locus_tag="WGO87_001694"
     CDS             complement(2934..5066)
                     /locus_tag="WGO87_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0393031.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPATFKSK
                     DVPTSPPAGPSVETTPVNQPAVGAAMRLPRRNIAAFDKNGNEIPNSKQAEEYLPLKEE
                     DILFLDGTPKEQADKLKKEINGRYPNVKIYTSDLKDAVYKYEHVRAGYVYTRFGTDEI
                     EQNSGGKQVTHRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDVKRHRAGKAVG
                     IDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDQKTLNGKLIKNQYVQRKTD
                     EKKPLTIYDITAKLDGNRFTGSAKVNPDLAKNPAGNERLFFHADADQRLEGGFFGDNG
                     EELAGRFISNDNSVFGVFAGKQKTDASNASDTNPALPSGKHTKILDSLKISVDEATDD
                     HARKFAISTMPDFGHPDKLLVEGREIPLVSQEKIIELADGRKMTVRACCDFLTYVKLG
                     RIKTERPAVQPKAQDEEGDEEDTGVDSVEEGEDEIDDEDEVSEEDAEEIEEEADEVEE
                     EEAEADEAEETEEESPTEESGSGSNAILPAPEAPKGRDIDLFLKGIRTAEADIPKTGK
                     AHYTGTWEARIGVPDKKDDQPGTTFIQKDSYANQGAKAEFDVDFGAKSLSGKLTEKND
                     THPAVYIENGVIDGNGFHATARTRDNGIDLSGQGSTSPQSFKANNLLVTGGFYGPQAA
                     ELGGNIIDSDRKFGAVFGAKKDNKEVEK"
BASE COUNT         1126 a         1414 c         1224 g         1459 t
ORIGIN      
        1 ggaaatcttg tttatatcga atcaaaaaaa cctgcacctt aatcagttgg cggtttagtc
       61 cgacttttgg ggtgcagatc aagctttcag acggtatttc ctttaaaact tcatttcaag
      121 cgcgaggctg aagtttctgc ccggtgcggc ataccttcca tagttgctgt cgccgccgtg
      181 ccggtttgcc gtgctttccg cagtctggcg caaggattcc caagtaacgt agcggtagtt
      241 gccgatattg tagatagccg ccctcaaggt cagccgtttt ttcagattca gataggcgga
      301 aacgtctgcc gtcgaccaag aagacgacgc tctttttgtc gaatatcgtt tttgatcgcc
      361 tgccagataa gcaagctcgt cagggttttt ccctttggaa taggtcagca taatgtttgc
      421 gccccatttc ccctcgggct ggtcgtatcc gaaccccaaa acataacgcg acggctgtac
      481 cgcatccaaa gcatagctgc ggagggacaa gtccggccgg ttggataccg atttcggttt
      541 gatacggttg tacgccaaag tcgtgtacaa accttcgggc agtttgccgt acacgccgtt
      601 ccagtcgatt tttcccaata tattaacgcc ttgaagcgac atattttggg cattgtaata
      661 atcgcgtata tcaatctctg tcaatcgtcc tgctgaatcc ggcaatttgg ttttgtgatc
      721 ggcaacggca atcatatcgg tataacggtt gcggaagctg ctgatttcca aaaagccgaa
      781 atcgcccttc cactgcaaac cgatttcgcg gttggctgcc ttttccgatt tcagggcggg
      841 acgctgccag cctttcggat aatcgtgata aatgtctatc ccgaaaagtt cttggaatga
      901 gggcgttctg aagccgctgg aggcgcggta agacacggaa aaatgccggt tcggtttgaa
      961 cacgatgccg ctgttccacg aacggtcaac ataccgcccg ctgcggacga gttcttccga
     1021 cgtggtgaag tttttccggt cgtacctgcc gcccaagctg aaatcaaaat atttgccgat
     1081 tgaaaaacga tcgttcaaag aaatgtggat attgctgccg ttgatttttc ttggcacgca
     1141 tttgcgggaa cgcagggttt cgatgtagcc gcagaccgat ccttcgacga cttcgggctt
     1201 acccaaaaga tacttatctt gattcttttc atcgaatccc gtggattccg aaatccttgc
     1261 cgcattgtgg gaaagctgtt cggggcggga gatcgctttg gaagcatcgt aaccgaagcc
     1321 caaagtcaga tgatgtttcg tccatttgtt tttcagcgat ttctcaaacg aggcattcaa
     1381 aacattgtgc tgttcgcggt agtggaaacg gtcgctgctg tcgtaggaat acggcttgtc
     1441 cgccgacgcg cggcaggatt tgtccacagc aggatacacg gcacaattca gcttcagcgt
     1501 gttgttgtcg gttgccacgc cctgtttgtc aaacgacaac accgccttgt ccgcccaatt
     1561 gtcagaatac gcttcgtttt cataacggta cagcaaaccc atacggcggc ggcggtgatg
     1621 ttcatcaata aatttggtgc gggaatattt caaacctatg cccctgacca actttttatc
     1681 gcccttccac tcttctatat tcggcacaaa atacaagccg tcgcggaaat cgtcgccgtc
     1741 gtacaccccg ctcttgtctc taaacttttc cgcctcgtcc gtaccgtaat actgtttttc
     1801 cgtcatatcg cggatgtcgt aacgctgttt ggtatcctca aacacaccgc cgacataatg
     1861 cctgccgccg aagcggtagc ccagcttggc aagccacgaa ccgctgcggt aatccatcgg
     1921 atcgggcaat atcctgccgc cgcccgtgta agcttgggcg gacagatttt cgtggcgcac
     1981 ctgcgcctcc cgcacctgcg cctcttcttc agcacttaaa ggctgatttt gttcaatacg
     2041 ttcttttacc cagcggttga gctggttgtt caaatatttc ccgtagcccg ccaattttgc
     2101 cacgggcttg gattcacgct cgccctctac tgagaaaaat tgccctgttt tatcttggct
     2161 ttgaatatcg tatgtctggc ggaacgcgtc caaacggttt atgccgtatg ccaccccgtc
     2221 cgcaatatcg ccgtgcgggc gcgtttcccg cccctgacgt tcggttcgga ttagcagccc
     2281 ttcccaaccg tctttgctga accccgcgcc gagcgacttc ataaattggc ggtttttact
     2341 gccgtaggca gtttttgcct gtatccccca gcttttgccg tctgaaatca ggtctgccgc
     2401 ctctttggtg cggaaggcga ctgcgccgcc gagtgcgccg ctgccgtgat cggacgaacc
     2461 ggcacctttg tcgatttcca ccgtgctgat gttttcatat tcgatttcgt tgattgcacc
     2521 gctgccgccg cgtccgccgt atccgctcaa cgatccctgc acggtaaacg cctgtatttg
     2581 ggcaacgccg tcaaccgaaa ccgccacacg gtttttatcc acgccgcgta tcgagtagcc
     2641 gccgctcgcg ccgttgccct gttcgacaac cgccacgccc ggatcgtagc gcgtcaggtc
     2701 gcggataccg agtacctgtt ctttgttcaa cgtttccgac gttttgacga ttttgcccaa
     2761 accggtcgcc tctttcgatc gccgtcccac tttggcggca cggacggtaa cctctttcaa
     2821 ggattgggtc tgcgccgcat caggtgccgc cccccccgct tgggcagcat aagccggaaa
     2881 agcggttgca acagccaagg cagtcagggt aagctgaaaa ccgtgtttct tattcatttt
     2941 tccacctctt tattatcttt cttcgcaccg aataccgcgc cgaatttccg gtcgctgtcg
     3001 ataatattac cgcccaattc cgccgcctgc ggtccataaa agccgcctgt tacaagaaga
     3061 ttgttggctt tgaagctctg cgggctggtc gaaccctgcc cagaaaggtc aataccgtta
     3121 tcccgagtgc gtgctgtcgc gtggaaaccg ttgccatcaa tcacaccgtt ttcaatataa
     3181 acagcggggt gtgtatcatt tttttctgtc aacttacctg aaagcgactt cgcgccgaaa
     3241 tcaacgtcaa attctgcttt tgccccttga ttcgcatagc tatccttttg aatgaacgta
     3301 gtgccaggct gatcgtcttt cttatctggt acgccgatac gcgcttccca agtgccggta
     3361 tagtgtgctt ttccagtttt tggaatgtcg gcttccgccg tgcggatacc tttcaggaaa
     3421 aggtcgatgt ccctgccttt aggggcttcc ggagcgggca ggatggcgtt tgaaccgctg
     3481 ccgctttctt ctgtcggcga ttcttcttcg gtttcttcgg cttcatcagc ttcagcttct
     3541 tcctcttcaa cttcatcagc ttcttcttcg atttcttctg catcttcttc ggaaacttcg
     3601 tcttcatcgt cgatttcgtc ttcgccttct tcgacgctat caacgcctgt atcctcttcg
     3661 tccccctctt catcctgcgc cttcggttgg acggcggggc gttcggtttt tatccgtccg
     3721 agtttcacat aggtcagaaa atcgcaacaa gcacggacgg tcattttcct gccgtcggca
     3781 agctcgatga ttttctcttg gctaaccaaa ggaatttcac gcccttcgac aagaagtttg
     3841 tcgggatgac caaaatcggg catagtggaa atggcaaact tacgggcatg gtcatcagtc
     3901 gcctcgtcaa cggaaatttt cagagaatcc aagattttgg tgtgttttcc agacggcagg
     3961 gcaggatttg tatctgatgc gtttgatgcg tctgtttttt gtttgcctgc gaatacgccg
     4021 aatacgctgt tgtcgttgct gataaaccgt ccggcaagct cttctccgtt atcgccgaaa
     4081 aaaccgccct caagccgctg atcggcatcg gcatggaaaa acaaacgctc attaccggca
     4141 gggtttttcg ctaaatcagg attgaccttg gcactgccgg taaagcggtt gccgtccaat
     4201 tttgcagtaa tgtcgtaaat ggtcagcggt ttcttttcat cggttttcct ttgcacatac
     4261 tgatttttaa tcagcttgcc attcagagtt ttttggtcga aatcaaccgt atatttggca
     4321 ggatgctttt ccctttcgtc gacatcctta gccgcataag aagttgcacc aacatcgtta
     4381 ccataaaatg tgatataacc caaattgtca atgccaaccg ccttacctgc tcgatgacgt
     4441 ttgacatcgg tcatatattg ccagttacca gaatattcca ccgttcccgc actcggtaaa
     4501 gattgggaag gacgttctcc agaataatat acaaaaccat cataacctaa gcggtgggta
     4561 acctgcttac cacctgagtt ctgttcgatt tcatctgttc caaatctagt ataaacatat
     4621 ccggcccgga catgttcata tttatacaca gcatctttta aatcggacgt gtagattttt
     4681 acattaggat accgtccgtt gatttccttt ttaagtttgt cagcctgttc tttcggcgta
     4741 ccgtctaaaa acaggatatc ctcctctttg agcggcagat actcctctgc ctgcttacta
     4801 ttgggaattt cattaccatt tttatcaaaa gctgcgatat tccgccttgg cagccgcatt
     4861 gccgcaccga cggcgggttg attgaccggc gtggtttcta ccgaaggccc ggcagggggc
     4921 gaagtgggaa cgtccttaga cttgaaagtg gcggggtacg cggtcggcgt tgattcgaca
     4981 acaggctgta cgccgaaatt gccgccgata caagatgcca aaagtaaggg caacaagaca
     5041 atgccgccat aattcggttt acacatccct acttttcctc ctatttgatt aataataatt
     5101 atcattatat taatatgtac agataatatc aacccgtttt tatagtgaat taacaaaaat
     5161 caggacaagg cgacgagccg cagacagtac agatacattc cgtcattccc acgaacctac
     5221 atc
//
LOCUS       DAWFBO010000142         4871 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFBO010000142 DAWFBO010000000
VERSION     DAWFBO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.142"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            66..326
                     /locus_tag="WGO87_001695"
     CDS             66..326
                     /locus_tag="WGO87_001695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393032.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            433..957
                     /gene="nspA"
                     /locus_tag="WGO87_001696"
     CDS             433..957
                     /gene="nspA"
                     /locus_tag="WGO87_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0393033.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDASYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            1093..1482
                     /locus_tag="WGO87_001697"
     CDS             1093..1482
                     /locus_tag="WGO87_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0393034.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAIAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1560..2390
                     /locus_tag="WGO87_001698"
     CDS             1560..2390
                     /locus_tag="WGO87_001698"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0393035.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2405..3862
                     /locus_tag="WGO87_001699"
     CDS             2405..3862
                     /locus_tag="WGO87_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0393036.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3903..4292)
                     /locus_tag="WGO87_001700"
     CDS             complement(3903..4292)
                     /locus_tag="WGO87_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393037.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKHPEIYQRFQHFFPFVELPLL"
     gene            complement(4474..4797)
                     /locus_tag="WGO87_001701"
     CDS             complement(4474..4797)
                     /locus_tag="WGO87_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393038.1"
                     /translation="MNILASDRNYELNIEIFIDNIIHFQITDESYKVKFSEYLFENKE
                     KNDWDRNPAINYFFEIIDDSYMDWLKEESFDFFEKKYYKAYIFFFSDSVIEVISSTEP
                     VFYSK"
BASE COUNT         1206 a         1493 c         1031 g         1141 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaaaa ccgtatgcct gcacctgcaa
      181 caatccgaca gataccgctg ttttttccaa accgtttgca agtttcaccc atccgccgcg
      241 tgatgccgcc accaccattt aaaggcaacg cgcgggttaa cggctttgcc gtcggcaaag
      301 cagccggata ccgctacgta tcttgaagta ttaaaaatat tacgatgcaa aaagaaaatt
      361 taagtataat aaagcagaat tctttaacgg attcttaaca atttttctaa ctgaccataa
      421 aggaaccaaa atatgaaaaa agcacttgcc acactgattg ccctcgctct cccggccgcc
      481 gcactggcgg aaggcgcatc cggcttttac gtccaagccg atgccgcaca cgcaaaagcc
      541 tcaagctctt taggttctgc caaaggcttc agcccgcgca tctccgcagg ctaccgcatc
      601 aacgacctcc gcttcgccgt cgattacacg cgctacaaaa actataaagc cccatccacc
      661 gatttcaaac tttacagcat cggcgcgtcc gccatttacg acttcgacac ccaatcgccc
      721 gtcaaaccgt atctcggcgc gcgcttgagc ctcaaccgcg cctccgtcga cttgggcggc
      781 agcgacagct tcagccaaac ctccatcggc ctcggcgtat tgacgggcgt aagctatgcc
      841 gttaccccga atgtcgattt ggatgccagc taccgctaca actacatcgg caaagtcaac
      901 actgtcaaaa acgtccgttc cggcgaactg tccgtcggcg tgcgcgtcaa attctgatat
      961 gcgccttatt ctgcaaaccg ccgagccttc ggcggttttg ttttctgcca ccgcaactac
     1021 acaagccggc ggttttgtac gataatcccg aatgctgcgg cttctgccgc cctatttttt
     1081 gaggaatccg aaatgtccaa aaccatcatc cacaccgaca aagcccccgc cgccatcggc
     1141 gcgtacagcc aagccgtccg agcaggcgac accgtttaca tgagcggtca aatccccctc
     1201 gatcccgcca cgatgaccgt cgtcggcaac ggcgatttcc gcgccgaagc gcgccaagtg
     1261 ttccaaaacc tgcaagccat cgccgaagcg gcaggcggca cgctggccga catcgtcaaa
     1321 ctcaacgcct acctgaccga cttgggcaat ttcgccgtct tcaacgaagt gatggcggaa
     1381 tttattgccg agcccttccc cgcccgcgcc gccgtcggcg ttgcctcgct gcccaaaggc
     1441 gtgcaggtcg aagccgaagc cgtcctcgtt ttgaacgcat aattccacgt tcggactgca
     1501 tgatatttca aaaccatgcc gtccgaaccg caacactttc ccacttcaca acatctgcaa
     1561 tggcacatta cgcaatcggc gacatacaag gctgtttcga cgaactgacc gcgctgctcg
     1621 gcaaaatcgg tttcaaccac ggcacggaca ccctctggct gacgggcgac atcgtcaacc
     1681 gcggcccgaa atccctcgaa acgctgcaat tctgcatccg gcacgaaaac agtgtgcaaa
     1741 tcgtcctcgg caaccacgac ctgcacctgc tcgctgtcgg ctgcggcgaa ggtgcgccca
     1801 aacgcagcga cacaatcgaa cccatactca aacaccccga cggaaaaaaa atgctcgact
     1861 ggctgcgtgc gcaaccgctg ttgatacgcg agggcagccg cgtgatggta cacgccggca
     1921 tcctgccgca atggcgcata accaaagccg aatcgctcgc tagagaagcc gaagccgaac
     1981 tgcgcggcaa aaaatacgtc aaattcttct ccaaaatgta cggcaacaaa ccggccgcgt
     2041 gggacgaagg tttgaaaggc tatgcccgcc tgcgcttcat cgtcaacgcc ttcacgcgga
     2101 tgcgcgccct gacctttaaa aacgaactgg atttcgacta caaatccaca gtgaaaaaaa
     2161 tgccgcctta cctgcgcccg tggttcaaag cccccgaccg gcaaaacctc gaccgcacca
     2221 tcatcttcgg acactggtcc tcgctgggct acacgaatgc cgacaacgtc atctcgctgg
     2281 acaccggcgc gctgtggggc ggacagctga ccgccgtcaa cctcgaaacg gaagaaatta
     2341 cccaagtcca agccgccggc ggcatagact ggaaaagctt cgcaaaataa cggaccgccc
     2401 cactatgcac aaaatcctgc ccatcgccca cgtcctctcc cgactgggta tgctgttttc
     2461 ctttatcctg ctgatacccg ccgccctctc ctacgccttt tcggacggcg cgtacaccgc
     2521 cttcgccacc accgcgaccg ttaccctttc cggctcgtgc atcgtccggc tcgccaccct
     2581 ccggttcagg cgcgaactgc gcccgcgcga cggcttcacc ctcgtcctga tgttgtggct
     2641 ggcgtttgcc gctatggcgg cgatgccgat gtacctgtat ttcccgaata tgggctttac
     2701 cgacgcattt tttgaatcga tgtcgggact gaccaccacc ggcgcgaccg tcatccccca
     2761 cgtcgacggg ctcgccccct ccgtcaactt ttggcggcat atgctcaact ggctgggcgg
     2821 gatgggcatc atcgtccttg ccgtcgccat cctgcctatg ctcggcgtag gcggcacgca
     2881 gttattcaaa gccgaaatcc ccggcattga caaagaaagc aaaatgtcgc cgcgcatttc
     2941 ccaagtggcg aaaaaactct ggttcggcta caccctgatc accatcctcg cggcagcctg
     3001 cctgcatttt gccgggatgg gctggttcga tgccgtctgt cacgcgatgg cgaccctctc
     3061 gctgggcggg ttttccaccc acgatgccag catagcttat tacaactccc ccctcatcga
     3121 agcggtcatc atcgttttca ccattgtcgg cggcatcaat tttgccaacc atttcgccgc
     3181 cctcaacagc cgcagcctca aaacctattg gaaagacgaa gaatgccgga cgatgctgct
     3241 gctgctctcc ggcagcatcc ttgccgccgc cctgtacctg tggcacaccg gctattacgc
     3301 cggttttacc gaatccctgc gctacaccgc cttcaacttc gtctccatcg gactggcaaa
     3361 cgggctggcc aacaccgact tcgcacaatg gccgctcctg atttccctgt ggatgttttt
     3421 cctcgccaac atcctcgcca actccggctc gaccggcggc ggcatcaaaa ccatacgcgc
     3481 cctcgtcctg ttcaaattca gcctgcgcga aatgatggtg ctgctgcacc ccaaagccgt
     3541 ccgcaccgtc aaaatcagcg gcaaggccat acccgaccgc ctcgcgctga ccgttatgtc
     3601 cttcatcttc atctacttta tgaccgtcgt cctcttcagc ttcctgctga tggcgagcgg
     3661 tatggaattt accaccgcct tcaccgccgt catcgcctgc atcaccaacg ccggccccgg
     3721 cttgggcgaa gtcgggcccg ccggcaatta cgccggtttg gacgtgatgc agaaatggat
     3781 ctgcgttacc gccatgctct tgggcagact ggaaatcttc accgtcttca tcctcttcac
     3841 tcccgcctac tggaagaaat aaaaaatgcc gtctgaaaac ttttcagacg gcatcttgtc
     3901 ctttaaagaa gcggaagctc tacaaaagga aagaaatgct gaaacctctg ataaatttca
     3961 ggatgtttct ctaaggcttt taatgctttt tcttttgaaa taggcggatc atagacatct
     4021 atccccctta agaaggcaat gcccgttaat gcattttttt gatcatttga aatgtaacca
     4081 taattaacga ctccaattac atcataaatt tcccctatac tcttatcaaa atcaatcaaa
     4141 tcaaaaatat acaatggaat tgtatcaata ggagtatctc tgacaactat atccaaccat
     4201 aatttaaatt cagaaagatt aatagcatca tagaacatac agactattgc aaatcctaaa
     4261 tcttcacaat tttctttact aattttccac ataattattt acctttatcc attgcttccc
     4321 aaccgataca tccggtattt tagcttctcc ttttccgtca aacactcctt tggttacctg
     4381 ctttgtgtca aaaataccgt ctgaaagccg aatatcgttt cagacggtat tttgattgtt
     4441 gggtttttaa tcagagcttt gtttgataat ttgttatttt gaataaaata caggttctgt
     4501 cgagctgata acttctatta cagaatcgct aaaaaagaaa atataagcct tataatattt
     4561 cttttcaaaa aaatcaaaac tttcttcttt caaccagtcc atataactat catctattat
     4621 ctcgaaaaaa taatttatag caggatttct atcccaatca tttttttctt tattttcaaa
     4681 taaatattct gaaaatttta ctttataaga ttcatccgtt atttgaaaat gaattatatt
     4741 gtcaataaat atttcaatat ttaattcata atttcggtcg gatgccaaaa tgttcacatc
     4801 ttgttcggta acacaaattt tgtccacata aaatctgtga ttattgttag aaaattgtaa
     4861 tggtttaatc t
//
LOCUS       DAWFBO010000143         4863 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFBO010000143 DAWFBO010000000
VERSION     DAWFBO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.143"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(210..938)
                     /gene="rmpM"
                     /locus_tag="WGO87_001702"
     CDS             complement(210..938)
                     /gene="rmpM"
                     /locus_tag="WGO87_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0393039.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1302..2252
                     /locus_tag="WGO87_001703"
     CDS             1302..2252
                     /locus_tag="WGO87_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0393040.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2365..3099)
                     /gene="fnr"
                     /locus_tag="WGO87_001704"
     CDS             complement(2365..3099)
                     /gene="fnr"
                     /locus_tag="WGO87_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0393041.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3304..4725
                     /gene="hemN"
                     /locus_tag="WGO87_001705"
     CDS             3304..4725
                     /gene="hemN"
                     /locus_tag="WGO87_001705"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0393042.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1240 a         1331 c         1178 g         1114 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tccgaacctg caccccgatt ctgacgatag
       61 ccgtttcaga aaaccaccca aaaaaacaaa ccccgcaaaa gcgggggttt gtttcacatc
      121 atcgatgccg accgacgttt cacgaatatt gcaacagtac ctggtacaaa atccactaac
      181 ctcatgcatc ggcaagatat tgcctagcct tagtgttggt gatgattgtg tgccggcaca
      241 acctgacggg ttacgatgct gcggattttc acatccacac ggcggtcagg ttcgatacat
      301 gcaatcagag cctcacgttt tttggcttta gagactttcg cacccagttt ggcaacttcg
      361 gcttcacaaa cttgagtcat ttgcgcttga gattcgccca agccgacagc agaaattcta
      421 gaaacaggta cgccgttgct gaccaggttg tttgccacta cgtatgcgcg gcgttcggac
      481 agggcctgat tgtatttgtc agaacccata aagtcggtat ggccttcgac gcggacagat
      541 tggatattgg tttgacccag gcgttgcgcc aatactttca ggttgtcttg agcttcggcg
      601 cgcaatgaat ccttatcgaa accgaacagg gttttggcag acagggaaat ggtttcatca
      661 acatattgcg gagcctgctc cacaacgacg acaggagcgg gtgcgggttc gggttctggc
      721 tcgggttcgg gggcagcaac cgcatcgccg cattctacgc gaccttggct tgctttatca
      781 aagtaggcgt ttttccagca ttcgccatag ttgttgcgta cgatttcgtt cgactggccg
      841 cttacggtgt aaccctgaac ggacgcctcg cccgcaacag cagtgccgga agcgagcaat
      901 gcaacgaata atgcgcttaa tttcagctgt ttggtcattt tattccctca ttaaatttgt
      961 acagcagata cagaagcaac tgctgaattg ccaacataca ggccgctgta gaaacatggc
     1021 ggcaaatcgg atatttcagt ctccactata aagaagcagg gggaggactg tcaatactca
     1081 acgcctgaaa acaaccgtaa aacagcctgt ttcctgatag tctatcatgc cgaaaaatca
     1141 attccgtgtc attactttag ggggattttt tccatatcgc acgattgccg ttgcacaaaa
     1201 acaacaaaca caacggcaaa gccccatacc gtaccggcaa gaaaatatga taaattataa
     1261 acaatgttca gccacccgac acagacccac accgacccgc catgaaatta caacaattga
     1321 aatacgcctt agaagtttac cagcacaacc tgaacgtttc cgaagcggcc gaagccttgt
     1381 ttacttcgca acccggcatc tccaaacaaa tcaaattgct ggaagaagaa atcggcattc
     1441 agatttttat ccgcagcggc aagcgcgtgg tttcggtctc gcagccgggc aaggtggttt
     1501 tggatattgc ggaacgtatt ttacgcgatg tacagaacat taaaaatatc ggcagcgagt
     1561 ttaccggaca ggacagcggt tcgctgacgg ttgccacgac gcatactcag gcacgctatg
     1621 ccctaccctt gattgttgcc gattttgtga aacgctatcc gaaagtcaac ctgaccatca
     1681 aacaggggag ccctgccgcc atcgcacgta tggttacctc aggcgaggcg gacttggcga
     1741 ttgttacgga acggatagac gaccatcccg aactgggaaa acttccctgc tatgactgga
     1801 ctcatgcggt tatcgtaccg aacgaccacc ccttgctcga atgcagaaac cccctccgca
     1861 ttgaagattt ggcgaggttt ccgctgatta cttatgaatt tgcattcaat gcgggcagca
     1921 gcatcgcgcg ggcattttcc aaagcccgtt tggaacagcc ggatgtcgca ttggcggcgg
     1981 cggatacgga cgtattgaag acttatgtgc gcttgggttt gggcgtggga ctgatggcga
     2041 aaatggcgta caacccggat acggacggcg atttgcagct tgtggatgcg gcacacctgt
     2101 tcgagccgtc gccgacgtgg attgctttgc gcagcgatac ttatttgcgc ggatatgcct
     2161 acgactttat ccaagcgttt gcgccgcacc tgacacgcga gaaggtggat aggattctgt
     2221 acacgcccat cagcgaggat ttttcgattt aggcggctgc cggttttcag acggcacttt
     2281 gcggcagata caacaaacag gacagatgtt ttcgtctgcc ctgtgtttat tgagaatgcc
     2341 gtctgaaatg ttcgtacggg ttaatcaaat ggcgtgcgaa caaccggata ccatcttctt
     2401 caacacctgc agattgagga tcttgatgtg tttgtgttcg acggaaatca atccttcctg
     2461 atgaaattta gataatgtgc ggctgacggt ttcaagtttc agcccgagat aactgccgat
     2521 ttcttcgcgg gacattctta agatgaagtc gttggcagca aaacctcggg aataaaggcg
     2581 ttgggaaagg ttcaacagaa aggcggcaat ccgctcttcg gcgcgcatat tgcccaacag
     2641 cagcataaca ccttggtcgc gcacgatttc acgactcatc atgcggaaaa agtgggtacg
     2701 caggctgggg atattctgcc ccagttcttc gatgtgggta aacggcagtt cgcacacttc
     2761 gctgtcttcc aaggcgaccg cgtcgcaact gtgcacatgg gaacagatgc cgtccatgcc
     2821 gatgagttcg cccgacataa agaaacccgt tacctgatcg cggccgtcct gactggcgac
     2881 ggttgttttg aagaagcccg aacggatggc aaagagcgag gtaaaggctt cgccgacaca
     2941 gaacaggtat tcgccctttt tcaggcggcg gctttgacgg atgacggcat cgagttggct
     3001 gagctcgttg ggcagcagcc cgacaggcag gcagagttcc cgcaaagaac aggaagaaca
     3061 cagcgttttc atctgatgtg tagtattatg cgaagccata ccgtaccttt ttgtgcgctt
     3121 tgccccatca tgattatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgtc
     3181 cttatttaaa tttaatccac tatatgtgct tattgacaca tatcaagaca ggtttatcat
     3241 actgtggcat tctaccaaac tatccaaaat ttgactaaca tcataaaacc gcacacacac
     3301 accatgaaaa tcattcagat acagaacaat cacaatgtaa acgatgaccg ccccgagttt
     3361 gaccgcgcgc tgattgccag cctgcccgcc agcggcccgc gctacacttc ctaccctacc
     3421 gccgaccgtt tccatgacgg tttccgcgaa ggcgaatata tcaaagcttt acatttgcgc
     3481 ggtatgggcg cgttaaacaa accgctttcc ctttacattc acattccgtt ctgcaacacc
     3541 atctgctact actgcggctg caacaaaatc atcaccaaag acaaaagccg cgccgatgcc
     3601 tacatcgaat atcttgaaaa agaaatggaa ctgctcgctc cacatctgaa cggacggcac
     3661 cagcttgccc aactgcactt cggcggcggc acgccgacct ttttgagcga cgaacagatc
     3721 gaacgtgtct tccgcatgat acgcaaacat ttcgagttaa tccccaccgg cgaatactcc
     3781 atcgaaatcg acccgcgcaa agtcagccgc gacaccgtcc tcatgctcgg cagactcggc
     3841 ttcaaccgca tgagcatcgg cattcaggat ttcgacccca aagtgcaggc agcggtcaac
     3901 cgcatccaaa gttacgaaga aaccaaagaa gtcatcgatg cggcgcgcga agcggggttc
     3961 aaatccgtca gcgtcgattt gatttacggc ctgccgcacc agacttcgga aagcatcaaa
     4021 accaccatcg ataccgtttt gtcgctcgat cccgaccgcc tcgcccttta tcactacgcc
     4081 cacctgccgc acgtgttcaa accgcaacgc cgcatcgata ccgcctccgt ccccgacagc
     4141 gaagagaagc tcgatatgct gcaatactgc gtccaaaccc taaccgaacg cggctacgtc
     4201 ttcatcggca tggatcattt cgccaaacct gacgacgaac tctccatcgc cctcaaagaa
     4261 ggcttcctcc agcgcaactt ccaaggctat tcgacctacg cggattgcga tttggtcgcc
     4321 atcggcgtgt cgtccatcgg caaaatcggc agcacctatt cccaaaacga acgcgacatc
     4381 gatgcctact atgccgccat cgacgaaggc agactgccca tcatgcgcgg ttatcagctc
     4441 aatcaggacg acatcctgcg ccgcaatatc attcaggatt tgatgtgccg cttcgcgctc
     4501 gactaccgga tttacgaaag tatgttcggc atcccgttcg accgctactt caaagacgaa
     4561 ctggcggatt tggaaaaact cgccggtttg ggattggtgc gcctgaacag ccacgggctg
     4621 accgttaccc cgaaaggacg cttcctcatc cgcaacatcg ccatggtgtt cgactaccac
     4681 ctgcgccaca aggaaaccaa ggcgaaatat tcgcaaacgg tgtgattgcg tctaacgtac
     4741 aaatgccgtc tgaaaggctt tttcagacgg cattttgctg ccggcaggat aagtgttttc
     4801 aagaacaggc ggcggcatat cataacgttc cgcacctttg tgtccgaccg ttccgaaacc
     4861 aag
//
LOCUS       DAWFBO010000144         4828 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFBO010000144 DAWFBO010000000
VERSION     DAWFBO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.144"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(92..574)
                     /locus_tag="WGO87_001706"
     CDS             complement(92..574)
                     /locus_tag="WGO87_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0393043.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(727..888)
                     /locus_tag="WGO87_001707"
     CDS             complement(727..888)
                     /locus_tag="WGO87_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393044.1"
                     /translation="MSGQPEKHHTSPIEDKRKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(962..1423)
                     /gene="recX"
                     /locus_tag="WGO87_001708"
     CDS             complement(962..1423)
                     /gene="recX"
                     /locus_tag="WGO87_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0393045.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1491..2198)
                     /locus_tag="WGO87_001709"
     CDS             complement(1491..2198)
                     /locus_tag="WGO87_001709"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0393046.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            2605..3870
                     /locus_tag="WGO87_001710"
     CDS             2605..3870
                     /locus_tag="WGO87_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0393047.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3928..4734)
                     /locus_tag="WGO87_001711"
     CDS             complement(3928..4734)
                     /locus_tag="WGO87_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0393048.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1043 a         1102 c         1421 g         1262 t
ORIGIN      
        1 aatttaatcc actatatttg cttgatttca tccgtttgca atggaaacgg atggcgcgat
       61 gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggacatg tcttcagagg
      121 cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
      181 tcaggatttc cagcggaggg acagggacgg gtttgccgtc tttgaccgca accatcgtga
      241 agtagcagct gttggtatgg cggatttcgc cggtgcggat gttttgtgct tcgacacgga
      301 tgccgatttc catcgaggtg cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat
      361 cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgacagggta acgcaataat
      421 tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc aggagttcgc
      481 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
      541 gttcgtgcga aggcagttgg cgttgttgtg tcatagtgta cctgttttaa gaaagggtgt
      601 tttgaaatgg gtgcggggaa attttaggga atggggaagt ggaattcaag ctaaaaaaag
      661 ctatgatggg atagtctgcc gaaatggacc gccgatgccg tctgaagcct tcagacggca
      721 ttttgtttag gaaagccaaa aacggaagac caggaaaaac acgagtgcca atacggcgac
      781 ccaaacgacg agcatgaatc cggcaactgc ccgtcccata cggtaaaccg ggtttttgcg
      841 tttgtcttcg atgggggagg tgtggtgttt ttcgggttgt ccggacattt tgttctcaat
      901 cggaaaagtt ggaaacgggc gtaaggttat gttgccgtgc aaaaagattc aagaaggaag
      961 gtcagcagcc gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat
     1021 cggcatcgaa tccgcgatag gcgaggaaac gcgcctgttt ttgtttttct ttgaggtcgg
     1081 cggcggggtg tttgaatttt ttacgcaaca cggctatggc ggcctgtttt tcgcttgagc
     1141 ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt
     1201 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcatagcgca
     1261 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcctcttcgc
     1321 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgagttct tggcgcgaga
     1381 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcgt
     1441 ggggcaggtt tcagacggca tagcggcgca aaccggagcc gtccgaatgc ctactcttct
     1501 ttgtttttct gaggttgcag gttttcgtaa atttcgggca gcgagccgat aatccagtcg
     1561 gggcgggtcg catcgtcttg cgagagcagc gtcatatcgc cgtaaccgaa ggtaacgccg
     1621 acgctcaggc agccggcggc tttggcggcg atgatgtcgt tgcgcgagtc gccgaccata
     1681 accatgtttg caacatcgat acccaaaact tcggcggcgt gccgcagcgg cagggggctg
     1741 ggttttttct cgggcaggct gtcgccgccg agtatcaggc tgaagtagtc ggcaagtccg
     1801 agctgtttca acagttcgac ggcaaggatt tcgtttttgt tggtaatgac tgccagcggg
     1861 atgcccaaag atttgagcaa tgccagcccg gcttcggttt cgggataggg gcgggtgaag
     1921 acgctcaaat ggtcgcggta gtatttcata taggatacga aacctttttc ccacagttcg
     1981 gaatcggctt cgcggtcgcg gtcgttggtg aggacgcggt gaaccagttt gccgatgccg
     2041 tcgcccacat agctttcgac cactttggcg ggcagcggtt tcataccgag ttgttccaac
     2101 atcgcttctg cggcggcggc aaggtcgggg acggaatcgc acagtgtgcc gtccaaatcg
     2161 aaggcgacgg cttggacgtg ttcgatggct gcattcataa tctgatttcc tgtcgggttg
     2221 aaagggttta ttttaacatc gtttgcacag ggcttctaat gtatagattg ttaacaatcg
     2281 caacaggttt tgcaggcggt gttttcggat ttcggggcgg aatcgggcaa gggtttgaaa
     2341 tcccagcgat gttccaatta aatttttaaa aaataaaagg atgcctatat ctttatttaa
     2401 tttgacattt atttttcgga cgcgcgcgct gctttttcag gcgaagtccg ggctgattcc
     2461 gtgcggcttg aaaaataaaa tcatatatag attaatcaaa tcgggcttat gccgactaca
     2521 cgctattaca ttttgtcggg aaattggtta tagtaatgag cgaaatgcac gcccctgaaa
     2581 atctttttgt gaaaaggaag caaaatgtct gaagcccttg caaaagaaac cctcaatccg
     2641 tttgaaatcg cgcgcaaaca ggtcaaaacc gcctgcgacc ggctaaaaac cgatccggcc
     2701 gtttatgaaa tcctgaagag tccgacccgc gtgttggaag tcaattttcc cgtcaaactc
     2761 gacgacgaca cggtcaaaac cttcacgggc taccgttcgc aacacaacaa cgccgtcggc
     2821 ccctataaag gcggcgtgcg cttccatccc ggagtgaatc tggacgaagt caaagccctg
     2881 tcgatttgga tgaccatcaa atgctgcgta gcgggcattc cttacggcgg cggcaaaggc
     2941 ggcgttacct tggatccgcg cgattattcc gaggcggaac tggaacgcat cgcccgcgct
     3001 tattccgaag ccatttctcc gctgatcggc gagaaaatcg atattcccgc acctgatgtg
     3061 aacaccaacg gcaaaatcat gtcttggatg gttgatgcct atgaaaacgt ggtgaaacat
     3121 tctgcaccgg gcgtatttac cggtaaaccg gttgagttcg gcggctcttt ggcgcgtacc
     3181 gaggctaccg gttacggcgt gaacctcgcc gccgtccaag ccttggaaaa actgggcaaa
     3241 gacgtgaagg gtgccactta cgccatccaa ggcttcggta atgtcggtta ccacacaggt
     3301 tattacgcgc atcaatccgg cgcgaaagtt gttgcggttt ctactgttga cgttgccatt
     3361 tacaacgaaa acggcttgga tatggaagca atcatcaaag aattccaaac caaaggtttc
     3421 atcaccaatg aagccggcta cggtaaagaa atcagcaatg ccgaactttt ggctctggaa
     3481 gtggacgtac ttgccccttg cgcactggaa aaccaactga cttctgaaaa tgccggtaaa
     3541 gtccgcgcga caatcgtggt cgaaggcgcg aacggcccga ctacgcccga agccgacgtt
     3601 atcctgcgtc aaaacggcgt attggtcgtg cccgatattt tggcgaactg cggcggcgtg
     3661 gtggtatctt attttgaatg ggtacaaaat ctgcaaggct attattggga gtttgacgaa
     3721 gttcaggaaa aagaaaccgt cgtcctgcgc cgtgcgttcc gcgatatatg gaacttggcg
     3781 caagagtatg atgtcgattt gcgaaccgcg tcttacatga tgagtatccg ccgtgttgaa
     3841 aaagcgatga aactgcgcgg ttggtattga tgtttgaaca aaaaatgccg tctgaagcct
     3901 tcagacggca tttttgtgcg ggcgggatta atggtgcgaa tggccgattt ggaaggttaa
     3961 agtcgagtag ttcgcctgtt tttggcacac gctttgatcg gggaagtcgg ctttgtgttc
     4021 gacattggct ttccagaagc cttggcgcaa ggggatgatg tccacttcgc ctttgtcgtc
     4081 tgtgctgtcg gagaaagcct gtgcttcggt tttgtgcgtt ttgctgcggt cgctggtgtc
     4141 gaagccgtca aaggtggcgg taacggtggc attgggcagc ggttcgccac ggaacagaac
     4201 gcggactttg aagcgttcgc ctacgtgaat gttggcggga ttgtccagcg ggacgatttc
     4261 caagttttgt ccgaccggtt tggtgatgat ggcggtgtcc gcgctttcgt gtccgacgtt
     4321 gacgatgttt ttgccgaaca ttcgggtttg ttcgcaatag cttgcgtcag gcatttcttt
     4381 gatgcccgcc tgtttccagc ctgctttgtt ttttgaccag aaagtaggct gatattcggc
     4441 gatgacgagg taactgccgt ccttaacggg acggttgctt cggtactggt agttgtatgt
     4501 gccgcgttga atcatgtttt ccttgccttt ttcggtaacc agctgcatcg gtttgctgaa
     4561 gatgtgcagg cggtctttgg cgatgggttc gagttcggga aattcgccgt agcccaagtc
     4621 ggctttaagg tattcgccgc cgtgcgtgtg ggcggtttcg acccagacgc ggtgggcgtg
     4681 ggcggatgtg gcaaataggg cggaaacggc gagcagtgtc aatgttttct tcatggtgca
     4741 gcctttgtct cgtgtgggtc aaagattttg ttatgttata acaaaatagg tatcgtgtga
     4801 agacggattt gctgtctgaa aactgtct
//
LOCUS       DAWFBO010000145         4579 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFBO010000145 DAWFBO010000000
VERSION     DAWFBO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.145"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            24..260
                     /locus_tag="WGO87_001712"
     CDS             24..260
                     /locus_tag="WGO87_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ0393049.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIANRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            428..2203
                     /gene="ggt"
                     /locus_tag="WGO87_001713"
     CDS             428..2203
                     /gene="ggt"
                     /locus_tag="WGO87_001713"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0393050.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            2422..2617
                     /locus_tag="WGO87_001714"
                     /pseudo
     CDS             2422..2617
                     /locus_tag="WGO87_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2684..3934
                     /locus_tag="WGO87_001715"
     CDS             2684..3934
                     /locus_tag="WGO87_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0393051.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKLGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAVAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            4062..4510
                     /locus_tag="WGO87_001716"
                     /pseudo
     CDS             4062..4510
                     /locus_tag="WGO87_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1261 a         1138 c         1059 g         1121 t
ORIGIN      
        1 aaaggcacgc cccagtttcc ccaatgtgga ttttcagcgc gtgcagtaga ggtgctgacc
       61 caattggcag accctttgct tttgttaata tcctagaaaa cccagaaatt cgcagtactc
      121 taccactcat cgccaatcgg ccaacctttc cacagctttg ggttaatggt gagttaatcg
      181 gtggtagtga tattatccta caaatgtacc aatcaggtga gctaaaacca ctcattgaag
      241 agcatagccc aaaggtctga ttacttaata tcttaaaata aatatcaaaa aagactgctg
      301 atgacattag cggtcttttt ttgatggtca agattgatag tttggattga attatttagc
      361 catattaaat acaataatca gcatgccttg tatgaatcat caatcaaact caggcgaagg
      421 agtgcttgtg gctaaaacat atttattgac tgcattgata atgtctatga caatctctgg
      481 atgtcaagtc atccatgcca atcaaggtaa ggttaatact cattctgctg tcatcacagg
      541 tgcagacgct cacacgcctg aacatgcaac gggactgacc gaacaaaagc aggtgattgc
      601 aagtgatttt atggtagcgt cagccaatcc attagcaaca caagctggct atgatatctt
      661 aaagcaaggc ggtagcgctg cagatgcgat ggtggcggtg cagacgacac taagcttggt
      721 agagccacag tcgtcaggct tgggcggtgg tgcatttgtg ttgtattggg ataataccgc
      781 caaaacattg accacatttg atgggcgtga gacggcaccg atgcgtgcga cgccggaatt
      841 atttttggat aaagatggtc aaccattgaa atttatggaa gcggtggtcg gtggtcgctc
      901 ggtgggtacg cctgctatcc ctaaactgat ggagacaata catcagcgat acggtgtatt
      961 gccttgggga aaattatttg atacaccgat ccgcttggca aaacaaggct ttgaggtgtc
     1021 gccaaggctt gccatctcgg ttgagcaaaa tcagcagcat ttggcacgct atccaaaaac
     1081 agccgcttat tttttgccga atggtgtgcc gctacaagca ggcagcttgc tgaaaaattt
     1141 agaatttgct gacagtgttc aggcgttagc agctcaaggt gcaaaagctc tgcatactgg
     1201 taaatatgcc caaaatatcg tttcagttgt ccaaaatgct aaggataacc ccggtcaatt
     1261 atccttacaa gatttatctg attatcaagt ggtggaacgc ccgcctgttt gtgtgactta
     1321 tcgtatttat gaagtatgcg gtatgggtgc accaagctca ggtgggattg ctgtgggtca
     1381 gattttgggg attttaaatg aattttcacc caatcaggtg gggtatgatg ctgaaggttt
     1441 acgtctcttg ggcgacgctt ctaggcttgc gtttgcggat cgtgatgtat atttgggcga
     1501 ccctgatttt gtaccagtac ccattcgcca gttgatttct aaagactacc taaaacatcg
     1561 cagccagctg cttgagcagt cggataaggc attgcctagc gtatctgcag gcgattttat
     1621 tcatgagtgg gtgtcctcac aagcgattga gttaccttct accagtcata tatcaattgt
     1681 ggataaggcg ggcaatgtgc tatcaatgac gacttccatt gaaaatgcgt ttggctcaac
     1741 tttgatggca aatggctacc tgctgaataa tgagctgaca gactttagtt ttgagccaat
     1801 aaagcagggc aaacaggtcg ccaatcgtgt cgagcccggc aagagaccac gctcctcaat
     1861 ggcaccaacc atcgtattta aagcaggcaa gccttatatg gcaatcggct ccccaggtgg
     1921 tagccgtatc attggctatg tcgctaagac gattgtggca catagtgact ggaatatgga
     1981 tatccaagat gccatcagtg cacccaattt attaaatcgc tttggtagct atgaactaga
     2041 aacgggtaca accgccctcc agtggcaaca agcattaaat gatttgggct ataagactga
     2101 cgtgcgtgag ctaaattcag gtgttcaagc cattattatc gagccaagca ggctggttgg
     2161 tggtgccgat ccacgccgag aaggtcgagt gatgggtgat tagaggtgaa gcttgtattt
     2221 tgtgaatttg accgcattaa gttaatatta aaataggggg aaattgtgag taatcaaaca
     2281 aaaatacaag ccatcaagca agcttctgag cagatcttcg caatttgtga gacgccaaac
     2341 acagcattac aagccattca tctgatttta agacatggtg gggcaggaga gttgtcttgg
     2401 caagtggtgt atcagcgggt tatggctgat aaagatgtgg ttggtgctgg ttatttgatt
     2461 gattttgcac aaactgccga aaatttacca tttgatgtat tgcctttggg cagccttgta
     2521 ctaacataaa ggtaatgaga cgctaaaaac cagcatgtta aataaattac ctgataatgc
     2581 caaagaaaat ttacgcatta tggactatct gccttaggtt ttgaagtatt tttttataca
     2641 aaaaacactc atgataccct gattacacaa aggagccgag aacatgtttt caaaaagcgt
     2701 taccctcgca caatatgacc ccgatttggc agcagccatt gcccaagaag accaacgcca
     2761 gcaagaccac gtcgagctga ttgcctctga aaactacgtc agctgcgccg taatggaagc
     2821 gcaaggttcc cagctgacca acaaatacgc cgaaggctac cccggcaaac gctactacgg
     2881 cggctgcgaa tacgtcgata ttgtcgaaca attggcgatt gaccgcgtca aaaaactgtt
     2941 cggcgcgcaa tacgccaacg tccaaccgca ctccggctcg caagccaacc aagccgtgta
     3001 cgcttccgtt ttaaaactag gcgacaccat tttgggtatg tctctagcgc acggcggcca
     3061 cctgacccac ggcgcgagcg ttaatatttc cggcaaactc tacaacgccg ttacttatgg
     3121 cttggatgaa aacgaagtgc tcgattatgc cgaagtggaa cgcttggctc tcgaacacaa
     3181 acctaaaatg attgtggccg gtgcgtctgc ctacgcgttg caaatcgact gggcaaaatt
     3241 ccgcgaaatc gccgataaag tcggcgcata cctctttgtc gatatggcgc actatgccgg
     3301 tctggttgcc ggcggcgaat atcccaatcc cgtgccgttc tgcgacttcg ttaccaccac
     3361 cacccacaaa accctgcgcg gtcctcgcgg cggcgtaatt ttgtgccgcg acaataccca
     3421 cgaaaaagcg ttgaactctt ccatcttccc aagcctgcaa ggcggtccgc tgatgcacgt
     3481 catcgccgcc aaagccgtag cgtttaaaga agcgttgcaa cccgagttca aacaatacgc
     3541 aaaacaagtg aaaatcaatg ctgtcgccat ggcggaagaa ctggtcaaac gcggtttgcg
     3601 catcgtttcc ggccgcaccg aaagccacgt tttcctcgtc gacctgcaac cgatgaaaat
     3661 caccggcaaa gccgccgaag ccgctttggg caaagcgcac atcaccgtca acaaaaacgc
     3721 cattccgaac gatccggaaa aacccttcgt tacctccggc atccgcatcg gttccgccgc
     3781 catgaccaca cgcggtttta acgaagccga cgcgcgcgta ttggcgaact tggttgccga
     3841 cgtattgtct aaccccgaag acgaagccaa cctcgccaaa gtccgcgaac aagtaactgc
     3901 tttgtgcaat aaataccccg tttacggcgc gtaagccttt ttaaaaatat tccgccaagc
     3961 aatccaatgc cgcctgaaat ctcataatgt ttcaggcgga aacctttgca aaatccccca
     4021 aaatctccta aattcccacc aagacattta ggagatttct catgagcacc ttcttccggc
     4081 aaaccgcaca agccatgatt gccaaacaca tcgaccgctt cccactattg aagttggacc
     4141 aggtgattga ttggcagccg atcgaacaat acctgaaccg tcaaagaacc cgttacctca
     4201 gagaccaccg cggtcgtcct gcctatcccc tgctgtccat gttcaaagcc gttctgctcg
     4261 gacaatggca cagcctctcc gatcccgaac tcgaacacag cctcatcacc cgcatcgact
     4321 tcaacctgtt ttgacgtttt gacgaactga gcatccccga ttacagcacc ttatgccgct
     4381 accgcaaccg gcgacgcaag acgacaccct gtccgaattg ccggaactga tcaaccgcca
     4441 actgaccgaa aaaaacctaa aaatagagaa agcatccgcc gccgtcgttg acgccaccat
     4501 tattcagacc gagacctttg caaaattcct ttccctcccg acagccgaaa cccaaacaca
     4561 ggatttcggc tgttttcgt
//
LOCUS       DAWFBO010000146         4529 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFBO010000146 DAWFBO010000000
VERSION     DAWFBO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.146"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(86..1024)
                     /locus_tag="WGO87_001717"
     CDS             complement(86..1024)
                     /locus_tag="WGO87_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0393052.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1033..1908)
                     /locus_tag="WGO87_001718"
     CDS             complement(1033..1908)
                     /locus_tag="WGO87_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0393053.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2090..2166)
                     /locus_tag="WGO87_001719"
     tRNA            complement(2090..2166)
                     /locus_tag="WGO87_001719"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2130..2132),aa:Met,seq:cat)
     regulatory      2316..2415
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2506..4407
                     /gene="thiC"
                     /locus_tag="WGO87_001720"
     CDS             2506..4407
                     /gene="thiC"
                     /locus_tag="WGO87_001720"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0393054.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTADRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFTELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1104 a         1300 c         1196 g          929 t
ORIGIN      
        1 ttgcagccct gataaaaaat atggctgctt tggtaaaaaa tgccgtctga aaggttttca
       61 gacggcattt tgtttttaag aagcatcagc ggaagcggac gatttccctg tcttcgatat
      121 ggatgcgtac cgtatcgttt cccgatattt caccggcgtg cggcatatcg aggttcagcc
      181 acaggatgcc gtgttccgga tggaggacgg acaggctgaa cgattcgggc aaacaggtac
      241 gggataatac gcggcactcc atgccgtctt ggtcgaaacg caccgcatgt tgcggaatat
      301 ggcggtcatc gtcggtattg ggcaaaccca tcagtcgggc gacctgcacg ccggcaggcg
      361 tttgaaccaa ggtttcgggc gtaccgcatt gaaggatttt cccctcgtgc atgacggcga
      421 tttcgtccgc cgccgtgcag gcctcttcgg gcgaatgcgt taccaaaacg gcagggatgc
      481 cgcccttgcg gatgcgttcg gcagtcatgc ggcgcagcgt gccgcgcaaa tgcgtgtcca
      541 aactggaaaa cgattcgtcc aacagcagca gggaagggcg gacaaccaaa gcgcgcgcca
      601 gtgccaaccg ttgtttctcg cctccggaaa gtttttcagg cttgcggtgc acctcgtttt
      661 ccagtccgac ttcggcaagt gccgccatgg caaggcgttc ggcttcggct ttcggcattt
      721 tttgcatttt caaaccgaat gccgcatttt ccagcgcact catatgggga aacagcgcgt
      781 aatcttgaaa catcagcgaa atacggcgtt tttcgggcgg catacgggta atgttttccc
      841 cgttcagctg aatttcgccg ccgtccggcc ggacgatgcc cgcaatcata ttcagcaggg
      901 tggattttcc gcagcccgac cgccccagta cggcgagtat tttgccgcgc ccgacagtca
      961 ggcagatgtt gtcggcaacc gttttgccgc cgaagcgttt gcagagtccg ttcagttcaa
     1021 gcatggcgca tcctataaac gtatgccgtc cagccactcg gacagcggat gggcggcatc
     1081 aatcagaccg taacggcaaa acgcgtccag cgtaaccagt tgcgcgtcgt gcatcatgtt
     1141 tcccgacaac atggtggcaa ccaagctgcc gatgtccatt ttttcaaaac acgcaacctc
     1201 gccgtcctga ttttcaggca ggaaggtttc gggtaggacg gcatcgaata catacaggat
     1261 ttcattgtgc acaccccggc tgacggggcg caggctgtgc agccgagata ccggacggat
     1321 aaacggaaac agcgttttat ccaaaccggc ttcttcgctg ctttcgcggc acacggcttc
     1381 agacggcatt tcgccgccgg aaacaccgcc ggcggcaata ttgtcgagtt tgtcgggatc
     1441 gactgctttg tgcggactgc gcttgcctat ccagaaatgc catcggccgt ccgattcggt
     1501 cagtccgttg agatggacgg cgcggctgag cagtccgaac ggacggaaag cggcgcgttc
     1561 gagcgtgaac aaggggttgc cgccgccgtc ggtcaggtcg aaacactcgt tgcgccatcc
     1621 gtgaagcagc cccgccttgt tccatgtgcg ggcgaggtgc tgcaagcggc cgcccatatc
     1681 tgaccagccg tccgcattca ggaaaatgcc gtctgaagac tccgagcagc ctgcctccca
     1741 gtcttttttg acgcgctccg cccattccgg cgacagattg cccaaaggca gacggttcag
     1801 atacagcgtt ttccagcaac tttctgcacc gtaacttgct tttgcccact cgaacagggc
     1861 atcaaggtcg tgtttgctga cggattcggt aaaacggacg gtcggcatag tcggctttct
     1921 tgaagacaat gcggaagatt gtagccaaac tgcctgaatt tgcaaccgca aacagacggc
     1981 gggctgccgt tccgatattg cggcaggaag gaaaatccgg caaaaaacgg aggcggcagg
     2041 aaacaggcaa aacaaaaccc ctcggcattg aaccgagggg tttgatattt ggttgcggga
     2101 gcaggattcg aacctacgac cttcgggtta tgagcccgac gagctaccat gctgctccat
     2161 cccgcgtcag aagatgaaac tatacggcag attttttatg ttgtcaaaca ttatttccgc
     2221 gaaaaatcat aaaattttgc cgtccggtgg tttatgcctg atttgaaata ttattttctt
     2281 tacaaaagtt catgttcgtg atttaatttt ggttaacatt gaaacagggg tgctgcatga
     2341 tgttcaggcg gctgagaaat accctttaca cccgatctgg ataatacctg cgtggggagt
     2401 tttcacggat tctgcttttc agacggcatt ggttttcaaa tgccgtctga aaacgcaaaa
     2461 cgctcctgtt tctttattct aaacgagaaa acaggagcat tttttatgac tacgccaaaa
     2521 aaaaccgcca aaacttccgg caacgaagcg cgcgaacttg ccgacttgag cgaagacatc
     2581 ggtatccgct tcaaatatcc gaactcggaa cgcgtgtatc tgcaaggcag ccgcgacgac
     2641 atccgcgtgc ctttgcgtga aatccgtcag gacgacacct atacggcgca aggcgcggaa
     2701 gccaacccgc cgattcccgt ctatgacacc agcggcgtgt acggcgatcc agcggcacac
     2761 atcgacctga aacaaggtct gccgcacatc cgcaccgcat ggctgaacga acgcggcgat
     2821 accgaaatcc tgcccaagct ctccagcgaa tacggcatcg aacgcgcaca cgatccgaaa
     2881 accgcccatc tgcgcttcaa ccaaatcacc cgcccgcgcc gcgcgaaaag cggtagcaac
     2941 gtaacccagc ttcactacgc gcgccaaggc attatcacgc ccgaaatgga gtttgtcgcc
     3001 atacgcgaac gtttaaaatt agacgaattg tcccaaaaat cggaatacgc caaactcttg
     3061 aaacaacacg cagggcaaag tttcggcgcg aacatcccaa cccgtcccga ccaaatcacg
     3121 cccgaattcg tgcgccaaga aatcgccgcc ggacgcgcga ttatccccgc caacatcaac
     3181 caccccgaac tcgaaccgat gattatcggc cgcaacttcc gcgtcaaaat caacggcaac
     3241 ttgggcaatt ccgccgtcac ttccagcctg accgaagaag tcgaaaaaat ggtgtggtcg
     3301 ctgcgttggg gcgcggacac aattatggac ttatccacag gcgcgcacat ccatgaaacg
     3361 cgcgaatgga ttatccgcaa cgcgcccgtc cccatcggca ccgtgccgat ttaccaagcg
     3421 ttggaaaaaa ccggcggcat tgccgaagat ttgacttggg atttgttccg cgacacctta
     3481 atcgagcagg cggaacaagg cgtggactat ttcaccatac acgcaggcgt gttgctgcgt
     3541 tatgtgccga tgactgccga ccgcctcacc ggcatcgtat cgcgcggcgg ttcgattatg
     3601 gcgaaatggt gtttggcaca tcatcgggaa aacttccttt acacgcattt cgacgaaatc
     3661 tgcgaaatta tgaaggcgta tgacgtatcg ttcagcctcg gcgacggttt gcgtcccggc
     3721 tgcattgccg atgccaacga cgaatcccaa ttcaccgagc tgcacacctt gggcgaattg
     3781 acctccaaag cgtggaaaca cgacgtacag gtgatgatcg aaggtcccgg ccatgtaccg
     3841 ctgcaacgcg tcaaagaaaa tatgaccgaa gagttgcaac actgttttga agcaccgttt
     3901 tacaccctcg gcccgctcgt taccgacatc gcccccggct acgaccacat cacctcgggc
     3961 ataggcgctg ccaatatcgg ctggtacggc acggcgatgc tctgttacgt tacccccaaa
     4021 gaacatttgg gactgcccga caaagaagac gtgcgtaccg gcatcatcac ctacaaactc
     4081 gccgcccacg ccgccgacct cgccaaaggc tggccgggcg cacagttgcg cgacaacgcc
     4141 ctgagcaagg cgcgtttcga gttccgctgg cgcgaccagt tccgcctaag cctcgacccc
     4201 gaacgcgccg aaagcttcca cgacgaaacc ctgcccgccg aaggcgcgaa aatcgcccac
     4261 ttctgttcga tgtgcggccc caaattctgc tcgatgaaaa tcacgcagga agtgcgcgac
     4321 tacgccgaca agcaaaaagc ccaacggcag ggcatggagg aaaaagcggt cgagttcgtc
     4381 aaaaaaggag cgaagattta cagctaaaac gtcaagcaaa aaatgccgtc tgaaacccgg
     4441 aaaaaaggct tcagacggca ttctttcgct tgtgaaaata tagtggatta acaaaaatca
     4501 ggacaaggcg acgaagccgc agacagtac
//
LOCUS       DAWFBO010000147         4528 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFBO010000147 DAWFBO010000000
VERSION     DAWFBO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.147"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(57..1850)
                     /locus_tag="WGO87_001721"
     CDS             complement(57..1850)
                     /locus_tag="WGO87_001721"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0393055.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2095..3204
                     /gene="proB"
                     /locus_tag="WGO87_001722"
     CDS             2095..3204
                     /gene="proB"
                     /locus_tag="WGO87_001722"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0393056.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3217..4479
                     /locus_tag="WGO87_001723"
     CDS             3217..4479
                     /locus_tag="WGO87_001723"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0393057.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1039 a         1249 c         1247 g          993 t
ORIGIN      
        1 ccgcaatgcc gtctgaagcc tttttatgct tcagacggca tcgtctgttt tcacgctcaa
       61 atcgtaccgc tgccttgcgc tttcggtttg gcggcactaa attccagttt gctcaaagcg
      121 gaaaggtagg ctttggcagt ggcaaccaaa acgtcggtat ccgcgccctg accgttgacg
      181 acgcggttgc cgcgtgccag acggacgctg gtttcgcctt ggctttccgt accttgcgtg
      241 acggcattga cggaataaat ctgcaaagtc gcgccgcttt gcgccacgct ttcaatcgct
      301 ttgaaaattg catcaaccgg gccggaaccg gttgcggaag cgcgtttttc ttcgcctttg
      361 atgctgaaca cgatgtcggc gcgcggctct tcgccggttt cggtactgat tttttgggaa
      421 atgaatttgt agctttcggt attcatattg cccatttcgt ccgacaccag cgcgtgcagg
      481 tcttcatcga agatttcgcg ttttttgtcg gcgagttctt tgaagcgtgc aaatgccgcg
      541 ttcagtgcct cttcgctttc caactcgatg cccaaatccg ccagcttggt tttgaaggcg
      601 ttgcggccgg acaatttgcc caagctcaat cggttagccg accaaccgac cgattcggca
      661 gacataatct cgtaagtttc gcggtgtttc agcaccccgt cctgatggat gcccgattca
      721 tgcgaaaagg cattggcacc gacaatggct ttgttgggct gcacgggata gcccgtaatg
      781 gtggacacca gtttggacga aggcacgatt tgcgtggtat cgatgccggt ttccaagccg
      841 aacaagtcgt ggcgcacttt caacgccatc acgatttctt caaccgaagc attgcctgca
      901 cgttcgccca agccgttgac agtacattcc acctgacgcg cgccgccttt taatgcggca
      961 agcgaattgg caaccgccaa gcccaaatca ttgtggcagt gtgccgacca aacgactttg
     1021 ccgccgttgg gcgttttggc aatcagttcg cggaaaaatt cttcggtttt gtacgggatt
     1081 gaatagccga cggtatcggg aatattgatg gtggtcgcgc ccgcttcaat caccgcgccg
     1141 cagatttcgg caaggaaatc gatttccgaa cgcaacgcgt cttcgcagga aaattccaca
     1201 tcgtcggtgt attcacgagc gattttcacc gctttgaccg ccgcctcaat cacctgcttc
     1261 ggcttcatct tcaatttgta ctccatatgg atggggctgg tggcgatgaa ggtgtggatg
     1321 cgttttttcg gcgcgggcgc aacggcctca cccgcctgac ggatgtcccg ctcgatggcg
     1381 cgggacaatg aacagaccgt tgatttggta atggttttcg caatcgcatt gaccgcctcg
     1441 aaatcgcccg ggctggcagc ggcaaaaccc gcttcgatga tgtccacacc caatttttcc
     1501 agctggcggg cgacgcggat tttttcctct ttggtcatag cggcgccggg cgactgctcg
     1561 ccgtcgcgca gggtggtatc aaaaataatt acgcggttgg tctgtgtcat ttctgttctc
     1621 tccagagatt cgtttttttg taaaaaagca ttcaaatcca gcggcctgcc ttgcgcttcc
     1681 gccaacgcgg tcagcttttg cagttgcgcc agaggcagcg agccgcgcgt gcgccattta
     1741 tagatggcgg cggtcgtcgc ggcattttcg ggatcgtgct gtttcaacgc ttcggcaagc
     1801 gcgttcacgc cgccgaaata agcaaccaag cggtctatat ctaattgcat ggtagtcctt
     1861 gtctaaaata aatcagccta cagcagaaat atggacagga ttatacgcat tttggacaaa
     1921 acggcaatat aaatttgaaa aatctcaata agccgccata gattttatta attttataca
     1981 ttttgtccat tttattgcag acgcggcgtg attgttgaca attgcggcag aacagggcaa
     2041 attcggcggc acaaggtggc aacgagacag gacaaaccaa caaggacgga aacaatgaaa
     2101 tacaaaagaa tcgtatttaa agtcggcaca tcttcgatta cccattcgga cggcagtctc
     2161 tcgcgcggca aaatccaaac catcacccgc cagcttgccg cattgcatca tgcgggacac
     2221 gagctggtct tggtgtcttc cggcgcggtt gcggcagggt tcggtgcgct gggtttcaaa
     2281 aaacgtccgg tcaaaatcgc cgacaaacag gcttccgccg ccgtcgggca ggggctgctg
     2341 atggaagaat atacggcaaa cctgtcttca gacggcatcg tgtccgcaca aatcctgctc
     2401 agccgcgccg actttgccga caaacgccgc taccaaaatg ccggcggcgc actttccgtg
     2461 ctgctgcaac gccgcgccgt ccccatcatc aatgaaaacg atacggtttc ggttgaggaa
     2521 ttgaaaatcg gcgacaacga cacattgagt gcgcaagtgg cggcgatgat acaggcagac
     2581 ctcttggtgc tgctgaccga catagacggt ctttacaccg gcaacccgaa cagcaatccc
     2641 gatgccgtac ggctggacaa aatcgaacac atcaaccatg aaatcatcga aatggcgggc
     2701 ggatcgggtt cggcaaacgg cacgggcggt atgctgacca aaatcaaagc ggcaaccatc
     2761 gccgccgaat ccggcgtacc ggtgtatatc tgttcctcgc tcaaacccga tgcacttgcc
     2821 gaagctgccg aacatcaggc ggacggctcg tttttcgtcc cccgtgccaa aggtttgcgg
     2881 acgcagaagc aatggctggc gttctattcc gaaagccggg gcagcgttta tgtggacgaa
     2941 ggtgcggaac acgctttgtc cgaacagggg aaaagcctgc tgatgtcggg cattgccgga
     3001 atcgaagggc atttttcccg tatggacacc gtaaccgtgt acagcaaggc aaccaaacag
     3061 cccctgggca aagggcgcgt cctgttcggt tctgccgccg ccgaagacct gctcaaatcg
     3121 cgtaaggcga aaggcgtgtt catccatcgg gacgactgga tttccatcac gcccgaaata
     3181 cgcctgcttc tgaccgaatt ttagaaaagg aaacccatgt caaacacaca aaaacagctt
     3241 gcccttgcca aagcggcaaa aaaatccgtc aacacggcgg atacggaaga aaaaaaccgc
     3301 gcgctgcttg ctatggcgga cagtctggaa gcggcggcgg cagatatttt ggcggcaaac
     3361 cgtcaagact tggaagccgc ggcaggcaac attcccgaaa gcatgaccga ccgccttttg
     3421 ttggacggca aacgcatttg cgcgatggcg gacggcatta gggcggttac cgcactgccc
     3481 gaccccgtgg gcgaaatact ggaaacctcg actctgccca acggcttgga aatcgtcaaa
     3541 aagcgtgtgg cgatgggcgt tatcggcatt atttacgaaa gccgcccgaa cgttacgtcc
     3601 gatgcggcgg ctttggcact gaaaagcggc agcgcggtcg tactccgcag cggcaaagat
     3661 gcattccaat ccgcacgcgc catcgttgcc gccctgaaaa cggggttggc acaaacccgc
     3721 atcgaccccg acgcgctcca gttgattgaa gacaccgggc gcgagggcag ttacgaaatg
     3781 atgagggcga aagattatct agacctgctg attccgcgcg gcggggcggg gctgatacgg
     3841 gcggtggttg aaaatgccgt cgtgccggtc attgaaacgg gaacgggcat cgtccacatt
     3901 tatatcgaca aggacgcgga ttgggacaag gcgctccgta tcgtgtacaa cgccaaaacc
     3961 agccgtccgt ccgtgtgcaa ctcgatggaa gtgctgctgg tgcatgaaga cattgctgcc
     4021 gacttcctgc ccaagctcga acggctgttg gttcgcgacc gtatagaagc cggactgccg
     4081 cccgtccgct tccgtttgga tccgcaggcg gcgcggcata tcggcggcga agcggcgggt
     4141 gcagacgatt tcgataccga gtttttagac tacatcctcg ccgtgaaaac cgtcgcttcg
     4201 gtcgaagagg cggtcgggca catcgaaacg cacggcacgc accattcaga cggcatcgtt
     4261 accgaaaacc gccacgctgc ggactatttc acgacccata tcgattctgc cgccgtgtat
     4321 atcaacgcgt ccacgcgctt taccgacggc ggcgaattcg gcttgggttg cgaaatgggc
     4381 atctccacgc aaaaactcca cgcccggggc ccgatgggtt taaaagagct gacgagttac
     4441 aaatacatcg tacaaggcac gggacaggtt agggaataat ccgaattcaa acgtctgata
     4501 tagtggatta actttaaacc gatacggc
//
LOCUS       DAWFBO010000148         4523 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFBO010000148 DAWFBO010000000
VERSION     DAWFBO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.148"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            40..463
                     /locus_tag="WGO87_001724"
                     /pseudo
     CDS             40..463
                     /locus_tag="WGO87_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            569..1132
                     /gene="pgsA"
                     /locus_tag="WGO87_001725"
     CDS             569..1132
                     /gene="pgsA"
                     /locus_tag="WGO87_001725"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0393058.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1212..1287
                     /locus_tag="WGO87_001726"
     tRNA            1212..1287
                     /locus_tag="WGO87_001726"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1245..1247,aa:Gly,seq:gcc)
     gene            1313..1388
                     /locus_tag="WGO87_001727"
     tRNA            1313..1388
                     /locus_tag="WGO87_001727"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1346..1348,aa:Gly,seq:gcc)
     gene            1412..1487
                     /locus_tag="WGO87_001728"
     tRNA            1412..1487
                     /locus_tag="WGO87_001728"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1445..1447,aa:Gly,seq:gcc)
     gene            1509..1584
                     /locus_tag="WGO87_001729"
     tRNA            1509..1584
                     /locus_tag="WGO87_001729"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1542..1544,aa:Gly,seq:gcc)
     gene            1610..1683
                     /locus_tag="WGO87_001730"
     tRNA            1610..1683
                     /locus_tag="WGO87_001730"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1642..1644,aa:Cys,seq:gca)
     gene            1786..1875
                     /locus_tag="WGO87_001731"
     tRNA            1786..1875
                     /locus_tag="WGO87_001731"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1820..1822,aa:Leu,seq:taa)
     gene            1979..3031
                     /gene="sohB"
                     /locus_tag="WGO87_001732"
     CDS             1979..3031
                     /gene="sohB"
                     /locus_tag="WGO87_001732"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0393059.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDHLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3082..4007)
                     /locus_tag="WGO87_001733"
                     /pseudo
     CDS             complement(3082..4007)
                     /locus_tag="WGO87_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201178.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(4061..4492)
                     /locus_tag="WGO87_001734"
     CDS             complement(4061..4492)
                     /locus_tag="WGO87_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0393060.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1134 a         1069 c         1175 g         1145 t
ORIGIN      
        1 tatcgctggg tatggtttgc cgccataagc tgtctgctgt tgtccgccgt ttttattgca
       61 ccttacctga cggcatttca cgaacaagaa aaaacattcg aatatgcgga attgaccgtt
      121 accgccctca accgcagcgg acgggcaatc aaactggatg cagacgggca gcattaccgg
      181 ctttcctgct atgggttcga cagtttgtgc acaggcggca atatcggcag aaccatcagg
      241 gcggagcagg ttagagccgt tttaagcgaa accgtcggca aaggtttttt aaacggcgtt
      301 ctgttggaat accgcaacag tggcagtgtc tataagcaat aaagattttg cccgcacgga
      361 agaccgcctt gtcgaagtgt tggcacaacc tgccgttttc tgcctgaaac tgggtatttt
      421 gcttttattg cccgccattt tcctgcgttt gaaaagaatg tgaaaacaga ttggcggggc
      481 gggggaatgc ccatgcagca attttacgtt ttgggttatg aggataaccg ttataatcac
      541 aactttacag tctgcacaga gaaaaccgat gccttggaat ctccccatct tcctcacatg
      601 gttgagggtc ttgctcattc ctgtcatgac agcccttttt tacctgcctt ttccgtggtt
      661 cgcggaggaa acgattaatc tcaccgccgc cgtcattttt gccgttgccg ccttaaccga
      721 ctggtttgac ggatttttgg caagactgtg gaaacagact tccgatttcg gcgccttcct
      781 cgatcccgtc gccgacaagc tgatggttgc cgtatcgcta cttttactgg tcaaactcga
      841 ccggacctat gttttgtttg ccatgatcat tatcggcagg gaaattacca tttccgcatt
      901 gcgcgaatgg atggcgcaaa tgggcaaaag gaacagcgtt gccgtcgcca ccgtcggtaa
      961 gttcaaaacc gccgcgcaaa tgctggcaat tttcctgctg ctgctgaata ttcccgattt
     1021 ttacggattt aatcttgttg ttatcggcaa catattgatg tttatcgcat ctttgctgac
     1081 agtctggtcg atgctgtatt atctgaaaat ggcgtggaaa gaaatcgcct gaaaaaaaca
     1141 taaaaatagc ttgacggcaa aaacagaatc cataataatt gcgtctcttc gatgttggag
     1201 aatgaaatcg ggcgggaata gctcagttgg tagagcgcaa ccttgccaag gttgaggtcg
     1261 cgagttcgag actcgtttcc cgctccaaaa atttcatttt cccacaatca ttgcgggaat
     1321 agctcagttg gtagagcgca accttgccaa ggttgaggtc gcgagttcga gactcgtttc
     1381 ccgctccaag ttttatttca aacatatcaa cgcgggaata gctcagttgg tagagcgcaa
     1441 ccttgccaag gttgaggtcg cgagttcgag actcgttttc cgctccaaaa accgatatgt
     1501 ttgaacgcgc gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgcga
     1561 gttcgagact cgtttcccgc tccaagtttt ttagataagc cagttttagg gcgagatagc
     1621 aaagtggtta tgcagcggat tgcaaatccg tctacgccgg ttcgattccg actctcgcct
     1681 ccattatcgt actatggcgg ggtggcagag tgtttatgct atgaggagtg caatcttcat
     1741 ataggccggt taaaatccgc gcccccgcct ccacctttca caaatgcccg ggtggtgaaa
     1801 taggtagaca caacggactt aaaatccgtc gggactaaac atcccgtgcc ggttcgattc
     1861 cggctccggg caccaagctg gaaatgacaa taccgctcca agcggttatt tttttatctg
     1921 tcggacggtg atggacaata ttgacatgtt catgcctgaa caagaggaaa tccaatcaat
     1981 gtggaaagaa attttactga attacggtat tttcctgctc gaactgctta ccgtgttcgg
     2041 cgcaattgcg ctgattgtgt tggctatcgt acagagtaag aaacagtcgg aaagcggcag
     2101 tgtcgtactg acggattttt cggaaaatta taaaaaacag cggcaatcgt ttgaagcatt
     2161 ctttttaagc ggggaagagg caaaacatca ggaaaaagag gaaaagaaaa aggaaaaggc
     2221 ggaagccaaa gcagagaaaa agcgtttgaa ggagggtggg gagaaatctg ccgaaacgca
     2281 aaaatcccgc ctttttgtgt tggattttga cggcgatttg tatgcacacg ccgtagaatc
     2341 cttgcgtcat gagattacgg cggtgctttt gattgccaag cctgaagatg aggttctgct
     2401 tagattggaa agtccgggcg gcgtggttca cggttacggt ttggcggctt cgcagcttag
     2461 gcgtttgcgc gaacgcaata ttccgctgac cgtcgccgtc gataaggtgg cggcgagcgg
     2521 cggttatatg atggcgtgtg tggcggataa aattgtttcc gctccgtttg cgattgtcgg
     2581 ttcggtgggt gtggtggcgg aagtaccgaa tatccaccgc ctgttgaaaa aacatgatat
     2641 cgatgtggat gtgatgacgg cgggcgaatt taagcgcacg gttactttta tgggtgaaaa
     2701 tacggaaaag ggcaaacaga aattccgaca ggaactggag gaaacgcatc agttgttcaa
     2761 gcagtttgtc agcgagaacc gccctcaatt ggatattgag gaagtggcaa cgggcgagca
     2821 ttggttcggt cggcaggcgt tggcgttgaa cttgattgac gagatttcga ccagtgatca
     2881 tttgttgttg aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc aggagaagca
     2941 aagcctgatc cagcgcattg gtttgcaggc ggaagcttct gttgaaaagt tgtttgccaa
     3001 acttgtcaac cggcgggcgg atgtgatgta gtttgccgaa cggatggaaa tgccgtctga
     3061 agcgtgttca gacggcattt ttcaagttcc ggctttgatg ttccgttccg accactctat
     3121 ccgttgtccg atgtctttca aattggaata ttgttcgacc aatcgcggca cgtcgtccaa
     3181 actcatggca aacatccaca gataagtgcc gaccgaatag acatgccccg cgctgctgta
     3241 gcctttgagc gtcatcatca caaaagcaaa gccgaacaaa atacccatcg ccgtgccgac
     3301 gcagagatag ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg caagcagtcc
     3361 gtaatggcgg tacagctgcc gccggtcgcc ttttcggata aagtggttgt cgcgttccaa
     3421 gctgttcttc aggcggaaat acaggttttc gctgatggcg gcaaaacgtg gcaaaagcca
     3481 taaaaacaac gcaagtatgc ccaccgccga cacgccgacc caaaattcca gcaccagcag
     3541 catgatgcac gcgccgaata tggatacgac ggatgtcgcg gcaatcggca ggtgttcttc
     3601 aaaaaagctg acaaactcac gcgacagggc aacccgtgca gttatagtgg attaacaaaa
     3661 atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggcaaggc gagacaacgc
     3721 cgtactggtt taaatttaat ccactatatt gccgaatgcg ggacttaccg ctgccgctgt
     3781 tccaacacaa ccggcacggc gatttcggta taaatccgcg taaacgtgcg tgtatcggca
     3841 atccgccgcg ccgcaccgac cagccacatt aaaagcacaa ccaaagcgta cagcaacgcc
     3901 tgccacacct tccccgcaat tacggcatta attgcccagc cgccaaacac cggatacacc
     3961 agcatcaaaa ggttttccag tccgaccaga aaaaatgtgc caatcaatcg cttgcggtgg
     4021 ttttggctat gtgtttcaac attttccaca tctttgctcc ctatttttta ttttccgaga
     4081 ttgttttttc catcacttcc gccagtgcat ccaaatcggc aaacagccga gttgtgcgct
     4141 tgtcgccgaa tgtggcaaat actttgtcgc tgaattcctg cgcgctttct gttaaaggtg
     4201 cggcataggc tttgcctgtt tcggtcaacg acagcaaccg tttgcgccgg tcctgttcgc
     4261 cttcctgcca ttcaatcaac ccttgtccgg caagggtttt gcatacgcct gaaacggtct
     4321 gtttgggcag gctccacttt tcgccgatat gcttttgcgt gcggctgcct tcggttgcca
     4381 gggtataaag tacggcaaag aggttgtaat tcagatcctg ctgcccaatc catttgtcga
     4441 agacattgca aatcaggttg atacgggtgc caagttggtc gagttggttc ataattggtc
     4501 ttgatattga ctaaatttgt gcg
//
LOCUS       DAWFBO010000149         4476 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFBO010000149 DAWFBO010000000
VERSION     DAWFBO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.149"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(505..611)
                     /locus_tag="WGO87_001735"
                     /pseudo
     CDS             complement(505..611)
                     /locus_tag="WGO87_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1030..1800)
                     /gene="xth"
                     /locus_tag="WGO87_001736"
     CDS             complement(1030..1800)
                     /gene="xth"
                     /locus_tag="WGO87_001736"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0393061.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPALPDDPQRRVIAATVSGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1860..2141)
                     /locus_tag="WGO87_001737"
     CDS             complement(1860..2141)
                     /locus_tag="WGO87_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0393062.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRVA"
     gene            complement(2548..3429)
                     /gene="nadC"
                     /locus_tag="WGO87_001738"
     CDS             complement(2548..3429)
                     /gene="nadC"
                     /locus_tag="WGO87_001738"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0393063.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLACLAFQTMDPSVRFQAEIQDGQAVRAGQTLAVVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHLHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(3505..4443)
                     /locus_tag="WGO87_001739"
     CDS             complement(3505..4443)
                     /locus_tag="WGO87_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393064.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1037 a         1106 c         1192 g         1141 t
ORIGIN      
        1 cgtttcatga aactgatgca gatatagtgg attaacaaaa atcaggacaa ggcgacgaag
       61 ccgcagacag tacagatagt acggcaacgc tgtactggtt taaatttaat ccactatatt
      121 ttgtaatagc tgccattatg cctactagga tatatacctt ctatataatc cttatttaaa
      181 taaaaaccct tataaaaatc tgtgttatct gtcgggattg aaaaaaagat tttatctttc
      241 tcatcaggtt tatatttgct atcaatatca aaaagagaat aaggattaca atgagttcga
      301 catactaggt aaaaatttat atcttggatc atgaaatatt cgatatttat caggatcgaa
      361 agatgctaaa aattcatcaa tattggtact ggatattttg tcatcactaa taaacatcgc
      421 tagagatggg cgactttgat tttccatttg ttgattagta aaggatataa atactaactt
      481 gccattgtca aaccacatag atttatatag tggattaaat ttaaaccagt acgtcgttgc
      541 ctcgccttgc cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt
      601 aatccactat agttattgcc tttgggagaa tcaattctga cagcacataa catagattta
      661 tctttacagt cgttatattg agtttcatca aactcaactg catggcacaa taaaggcaat
      721 aggaaaaaca gggttattaa tttattacta tatataagac ggctttctat ttattcgaaa
      781 ataaatctag ttttcaatat ttgattttgt tttggattag tgtacataat aaattttact
      841 gctccaaaat ctgatattaa ccctatgtat ttcataaatt tttcaggaaa ataggctgtg
      901 tttgaacaat ccgccgattt cggctgtttg ccggggaaag gcaattattt ttcagtcaga
      961 tagaaagcta tcctattgaa taaattgaag ataaggatgc ttgtcaacgc catatcaaaa
     1021 cacggtcttt taccaatcga attctgccgt caccggcgcg tggtcgctcg gacgctccag
     1081 cgcgcgcgtc tccaaatcga cgcggacatc cttcaacgcc gccgccatcg caggcgacac
     1141 caaaatatgg tcgatacgca ggcccagttt gcgttggaac atcgcgccgc gatagtcgaa
     1201 ccaggtatag aacgcgcctt cgggatggac ttggcgcagg ctgtcggtca gtcccaaatc
     1261 cagcaggttt tgaaaccact gccgttcgac ggacgaacag tggatttttt cgtgccattt
     1321 ttcagggtcg taacagtccg catcggcagg cgcgatattg aaatcgccca gcaacaccag
     1381 tttgccgtgg cgggtcattt catcgcggac aaactccgtc agtgcggcaa accactgttc
     1441 cttatatttg aatttggggc tgtccaaagc ctcgccgttg acgcaataga cattgatgac
     1501 gcgcacgccg ctgacggttg ccgcaatcac gcgccgttgc ggatcgtccg gcagtgcggg
     1561 caaaccgaaa tgcacgtcct gcggcacgct gcggctgacg attgccacgc cgttgtaggt
     1621 tttctgcccg ctccaaacac agtgccagcc catcatttgc aaagcggcgg ccggaaattt
     1681 gtcctgatcg agtttgagtt cctgcaaaac caaaatatcg ggcggattgt cggcaagcag
     1741 gttttgcacc tgcggcagcc gcacattgag cgaattgaca ttccaagtgg tgattttcat
     1801 aacatttccg aaaaatgctg tctgaaacgt tcagacggca aaaggaaagg attctaacac
     1861 tatgccacgc gtttgttttc caaatcgcga accgtgtgca gaatcgccgc cgcttcttcg
     1921 gaaaccaggc ggtattcgat aatgcggtgg taacgcgtaa aatcgactag accttccacg
     1981 cgcaggcggt tcaaatggtt ggaaactgcg gtggccggca gggataagga ttttgccagt
     2041 tcggcgatat tgcgttcgct gtccaacaat tgaatcagta tcgccatacg ttcgggatgc
     2101 gccataagtt tgagtatggt gtggagcggt attgtgttca tcggtattcc taaaaccttt
     2161 gccgcccgcc aagcggattg acgggcggca gatatggatg attgtgatta tctttataaa
     2221 tcgtattata aatcaaatat ttatggatga ttgtacagga ttgtatgttc cttttcccac
     2281 tttttttgca aacaagcgta aaggcgggct ttcagacggc ataaaactgc cgccccccgt
     2341 cctttttcct gccctaccga tgccgccgcg ctgtcgggca aacaaaatac ctgcctgaaa
     2401 tgctgttttt tctaaagcat cacgaccttt acgaaaaatg ccgtctgaag aatctttcag
     2461 acggcatttc ctccctgagg caataaccgg attagggtta agcggttcct tgcctgacat
     2521 atcagacagc cgcccgcacc ctaaaactca cgccacgaaa tccaaaccta tgtccaacga
     2581 acggctgctg tgggtcagat agccgagggc gatgccgtcc actccggttt gcgccacgcg
     2641 cttcaggcgg tcgaagccga tgccgcccga tgcttcgcaa tagatggtgt ggaggtgggc
     2701 ggtttgcgtg tggcagcggt ttgccgcttc tttcagggtt tcgtcgtcca tgttatccag
     2761 caaaatccgt tccgcgcccg ctgcgatggc ttcgtccagt tgtgccaacg tatccacttc
     2821 gatttccacg caggtcaatg ctccgacagc ctgttttgcc tgctgcacgg cttgggcgat
     2881 gctgccgcaa taggcgaggt ggttgtcttt gatgagcacg gcgtcgtcca agcccatgcg
     2941 gtggttcacg ccgccgcctg ccctgacggc gtatttttgc aggacgcgca gcagggggat
     3001 ggttttgcgg ctgcacacga tgtctgtacc gtattcggcg acttcggcaa cggcacgcgc
     3061 ggtggcggtg gcgatgccgc ttaagtgcgt gaggtagttg agtgcggtgc gttcggcggc
     3121 gagcagcgcg caggcgttgc cttcgacgac ggccagcgtc tgacctgcgc ggacggcttg
     3181 tccgtcttgg atttcggctt ggaaacggac ggacggatcc atcgtctgaa aggcgagaca
     3241 cgccaagtcc ataccggcga taacaccgtc ttcgcggctg acgaggaaga gtttggcggt
     3301 tttgtcgggg gcgataacgg cggcggacgt aatgtcgccg cgcctgccca agtcttcgct
     3361 caaggcttgt tcgactatgg ggcgcaacag ggtgtcgggc agggaaaaga gggtgttttc
     3421 agacggcatg aggtttcctt tgtcgaaaaa gattgatgtt gcaccgatgg ggcaaaactt
     3481 catttgatgc cgtctgaaaa cgggctaacg gctgcccagc ggcagtccga tgtccgaaat
     3541 cagcctgtcg ggcaggacgt cgtcgcggaa gcggcaaagc tgcgcgggac ggcctttgct
     3601 gcccgatacg ccggtatccg acggctcgat gaggttttgc tgctgaatct ggcggcggaa
     3661 gttttgcttg tgcagcaatc ttccgctgat ggcttcgaca ctgttttgca gttgcagcag
     3721 cgtgaattcg ggcggcatca gctcaaaaat cacggggcgg tatttgattt tggcgcgcag
     3781 gcgggacagg gcggtcgcca atacgcggcg gtggtcgtgg cgcatgggct gccctgtgag
     3841 cgcgaaatcg aagtttgcct gcggctcggc ggcttccact atcaggccgc tttcatacag
     3901 catttcatag cgttgcaaaa cgtattcttc cgaccagttt tccggttcga ccccccaaca
     3961 caaatgaatg cgcttgagcc gcatttgacg cacttcctcc gtgtccgccg agtttgccca
     4021 aatgcgcagt cggctgacga cggcgtcgcg ctgtccgccg tcggtgcgca agtcttccca
     4081 cgggaaatag ccgtagcagt cctgccattt cgcatcggga tgcaggatgc tgtcggctgc
     4141 ctcgcgcacc agccccaaat agctgacgta caagacgggc atgccgtgtt cgttgcggcg
     4201 gtgggtatcg acaaaggtgt aaagctgttc cacatagccc ataggctgcg aagtctgctt
     4261 ggcaacccac agcttcacgc ccgcctgcaa ggaattgcgc agaggggaga gcgggccgtt
     4321 gggcaggagt gtgccttggg cgacggttaa tacccgcagg ccgccgtcgg taacggcaat
     4381 caatacggga accagctcga cgatgctttg cggcggggct tcggcttcgg ggtaggcatc
     4441 catgctcggc tttctgcggg tgggaaaggc tttatt
//
LOCUS       DAWFBO010000150         4474 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFBO010000150 DAWFBO010000000
VERSION     DAWFBO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.150"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WGO87_001740"
     CDS             complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WGO87_001740"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0393065.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WGO87_001741"
     CDS             complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WGO87_001741"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0393066.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1964..3205
                     /locus_tag="WGO87_001742"
     CDS             1964..3205
                     /locus_tag="WGO87_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0393067.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGDVSLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWAAGGLIADAKPSVLFDTQAPAGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSISGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3355..4410
                     /locus_tag="WGO87_001743"
     CDS             3355..4410
                     /locus_tag="WGO87_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0393068.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HSIPEYALIEPHLVDQHRTLKKLRSFLFSSRLTQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPGETADLRAKYGFADYETVFLFPSTGHTRKGLELLADFFEHTGL
                     PVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDMLASV"
BASE COUNT         1055 a         1450 c         1029 g          940 t
ORIGIN      
        1 gtcctgattt ttgttaatcc actataaaat ggaaaatacc cggctatcgt ctcattttcg
       61 ttttaatcag ccataaaaat gccgtctgaa accctttcag acggcatttc tgtcaaacgc
      121 cggacgcact caatccaaac tcaacagcag gttgcggaac gcgttcgggt ctttgataaa
      181 cgtcatctcg cccgcctgcg gaaaatgaaa gtccaacagg cgcgacaccc aaaaacggat
      241 gcagccggca cgttgggcgg tcgggaaata cgccttttct tcggcactca aggggcgcac
      301 gccctcataa ccaccgataa acgccttttt caacgcctca tccaacttat tgtccgctgt
      361 ccttgcccaa tcgttgaccg caatcgccaa gtcatacata aaattgcccc ggcaggcata
      421 atagaaatcg atgaaacccg atacctgacc gccgtcaagc aacacattgt ctttaaacaa
      481 atcggcgtgg atgatgcccg aaggcagatg attgccgaga ttgtccttca gcgcatcgat
      541 ttcggaacac agcagcgcgg catcgtcttg cgacaggacg ggcagcagcc gggtgcacgc
      601 ctccgtccac cacgcatcgt aacgcgggtt ttccatttcc aaagggaaat cggcggcggc
      661 aaggtgcatt ttggccagca tcgcaccggt atggaaacac tgctccgccg tcggcagcgc
      721 ggtatccgaa cctttcaggc aggcaaccaa acaagccggc ttgcccgcca aaacggaatc
      781 aagccggccg tctttgcgcg caaccggcgc ggcaaccgcc acgcccttca tactcaaatg
      841 ccggttaagc tccagaaaaa acggcagctc ttcctgtttc aacacttcaa acacggtcag
      901 cacataacgt cccgaagtcg tcgtcagaaa ataattgctg ttggtaatcc cctgcgcgat
      961 gccctgcagg gaaacaaatt cccccaaatc gtaaccgctc aggaagccgc gcatttcatc
     1021 atcggaaaca ctggtataga cagacatatc aactcacttg ctcaaaaacg ggcaaacccg
     1081 ccgtcagaac gccggacggc aggcaaacat ataattcaca tcggtcgaat cgcacagggc
     1141 ataagtttgc gacaacacat ggtaagtcat acccttcgta tccgccacat ccaagcctgc
     1201 ctgacggcac attcgcgcca gctcggcagg tgcgatgaat tttttccagt cgtgcgtacc
     1261 tttggggaca aacttcaaca gatattccgc cgccacaatc agatgcaggt acgatttcgg
     1321 gtttttattg atggtagaaa aaaacaccat gccgtccggt ttgaccagat tggcacaagc
     1381 acgcacgatg gcggcgggat cggggacgtg ttccatcatt tccatgcacg ttaccacatc
     1441 gaacgagtgc ggttccgcct cggcaaggtc ttccacgcgg atacattcgt attcgatatc
     1501 ggcgacattg ttcaaagccg cgtgcaggcg ggcggtttcc aacgactgct ccgccatgtc
     1561 gatgcccttt acaaacgccg cgccgcgccg cgccatactt tccgccaaga tgccgccgcc
     1621 gcagcccacg tccaaaaccc gtttgccgca caaatccgcg tgtccgtcga tataatccag
     1681 ccgcagcgga ttgatgtcgt gcaaggtttt gaactcgccc gacttgtccc accatttgtc
     1741 ggcaatccgg ctgaatttgg cgatttcccc ctcatcgaca ttatattttt tgtcggacat
     1801 tttccctccc atctgacgaa ccgcccactc caaaacccaa gattatacta tggaacacaa
     1861 tgccgtctga aagccttttc gggcggcgcg gcgcaaatac cttacaaatc cttacacttt
     1921 acggcataat ggcggctcgc tttttctggc agaaagacaa aatatgccca acaaaacccc
     1981 ttcactgttc ggcggcgcga tgattatcgc cggcacggtc atcggcgcag gcatgctcgc
     2041 caacccgacc gccacatccg gcgtatggtt taccggctcg ctggccgtgt tgctgtacac
     2101 ctggttttct atgctttcca gcggcctgat gattttggaa gtcaacaccc attatccgca
     2161 cggcgcaagt ttcgacacga tggtcaaaga cctgctcgga cgcggctgga acatcatcaa
     2221 cggcatcgcc gtcgccttcg ttttatacct gcttacttac gcttatatct tcgtcggcgg
     2281 cgacctgacc gccaaaggct taggcagcgc ggcaggcggc gacgtttcac tcaccgtcgg
     2341 acaactcgtc ttcttcggca tcctcgcctt ttgcgtatgg gcatccgcac gcttggtcga
     2401 ccgcttcacc ggcgtcctta tcggcggcat ggtattgacc tttatttggg cggccggcgg
     2461 gctgattgcc gatgccaagc cgtccgtcct cttcgatacc caagcccccg ccggcacaaa
     2521 ctactggatt tacgccgcca ccgccctgcc cgtctgcctc gcttccttcg gcttccacgg
     2581 caacgtctcc agcctgctca aatactttaa aggcgacgcg cccaaagtgg ctaaatccat
     2641 ctggacgggc acactgattg cgctggtaat ttacgtcctc tggcaaaccg ccatccaagg
     2701 caacctgccg cgcaacgagt tcgcccccgt catcgccgcc gaagggcaag tctccgtcct
     2761 catcgaaacc ctgtccaaat tcgcccaaac cggcaatatg gacaaaatat tgtccctgtt
     2821 ttcctatatg gcgatcgcca cctcgttttt aggcgtaacg ctcggactct tcgactacat
     2881 cgccgacatc ttcaaatgga acgacagcat ctccggccgc accaaaaccg ccgcgctgac
     2941 cttcctgccg cccctgattt cctgcctgct cttccccacc ggcttcgtta ccgccatcgg
     3001 ctacgtcggc ctggcggcaa ccgtctggac aggcatcatc cccgccatgc tgctctaccg
     3061 ttcgcgcaaa aaattcggcg caggcaaaac ctataaagtt tacggcggct tgtggctgat
     3121 ggtttgggtc ttccttttcg gcatcgtcaa catcgccgca caggtattga gccaaatgga
     3181 actcgtcccc gtatttaaag gataaaggca aaatgccgtc tgaagcccgc cggcggcttc
     3241 agacggcatt gccgcaacaa acggcaaccg tattccggca cacagcgcat taccctgccc
     3301 ctcacgcaca aatcccgccc cgacaaaccg ggacgcaacc ataaaggaac aatgatgaag
     3361 ctcaaaatag acattgcaac caacaacttc aaacacggcg gcggcacgga acgctacaca
     3421 ttggatttgg taaagggtct gaacagacaa aacatcacac cggccgttta tgcgacgaaa
     3481 tttgatcaca gcattcctga atacgcccta atcgaacccc atcttgtcga tcaacaccgg
     3541 acgctgaaaa aactacgctc attcctcttt tcaagccggc tcactcaaac cagaaaaaac
     3601 agtgccgcca aactgattgc ctgccaccac gccgattacg ccgacctcct catctgcggc
     3661 ggcacacact tgggctacct gcaccatatg gcgcaaaaac cgaacctgct cgaccgcctc
     3721 gccatacgcc gtaaccgcag caactacgcc accgccaaac tgattatggc gcattcccat
     3781 atgatgcggc gcgaactggt cggactgtac ggcgttcccc ctgaaagaat ccaagtcgcc
     3841 cccccccccg cagatacgga acgcttcttc ccgcaacccg gagaaactgc cgacctgcgc
     3901 gccaaatacg gctttgccga ctatgaaacc gttttcctgt tcccatcgac cggccacacg
     3961 cgcaaaggtc tggaactgct tgccgacttt ttcgaacata ccggcctgcc cgtcaagctc
     4021 gccgttgtcg gctccccgct cccccgccct atgaaaaacg tcgtcgggct gggcttctgc
     4081 accgatatgc ccgaactcta ccgcgccgcc gactttacca ttatggcctc cctgtacgaa
     4141 cccttcgggc tggtcggtgt cgaatccgtc ctatgcggca cacgcgtcgt cctctccgaa
     4201 aacatggcat gtacagaggt catgaacgaa gaagccggat tctttttctc acgccaaaac
     4261 ccggaaaccc tggcgcaagc cgttgcccaa gccgtcagcc ttaaaaaaca gggcggacac
     4321 cgcctgtccg acccgatgcg tgcactgaac tacaacccgt ccctgtcgca ccacatcgac
     4381 cgactgaccg acatgctggc atccgtataa caaaatgccg tctgaacgag ttttcagacg
     4441 gcattgctgc gttatagtgg attaacaaaa atca
//
LOCUS       DAWFBO010000151         4416 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFBO010000151 DAWFBO010000000
VERSION     DAWFBO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.151"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            186..2033
                     /locus_tag="WGO87_001744"
     CDS             186..2033
                     /locus_tag="WGO87_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0393069.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPEGKTLADYSSYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAAMGNTQEAEKLY
                     KQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAVKRALVLFQNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREIA
                     GKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADIPI
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGTVPAR"
     gene            2322..2534
                     /locus_tag="WGO87_001745"
     CDS             2322..2534
                     /locus_tag="WGO87_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0393070.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2666..3112
                     /locus_tag="WGO87_001746"
     CDS             2666..3112
                     /locus_tag="WGO87_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0393071.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            3133..3282
                     /locus_tag="WGO87_001747"
     CDS             3133..3282
                     /locus_tag="WGO87_001747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393072.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(3311..3703)
                     /locus_tag="WGO87_001748"
     CDS             complement(3311..3703)
                     /locus_tag="WGO87_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0393073.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFSGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3774..4379)
                     /locus_tag="WGO87_001749"
     CDS             complement(3774..4379)
                     /locus_tag="WGO87_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0393074.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         1085 a         1239 c         1220 g          872 t
ORIGIN      
        1 cgtctgaaac gggcaaaggc agcggttctc accaaaacgg caaatgattg aaaaatataa
       61 attatgtcag aaacattcca aagccggtgc agtttcggca tataattcgg gcaaacgcct
      121 gttcagacgg cattttgtct tttccaaccc tgaccgttca gggttccgat tcttaaggaa
      181 atccgatgta cctaccctct atgaagcatt ccctgccgct gctggcggcc ctggtgcttg
      241 ccgcgtgttc ttcgacacac gcgcccagca gcaagaccct ggcagacaat atagaaactg
      301 ccggtctttc ggcaagcgtt cccacccgcc ctgccgaacc cgaaggaaaa acgctggcag
      361 attacagcag ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg
      421 ccgcctattt ggaaaacgcc ggcgacagcg cgatggcgga aaatgtccgc aacgagtggc
      481 tgaagtcttt gggcgcacgc agacagtgga cgctgtttgc acaggaatat gccaaactcg
      541 aaccggcagg gcgcgcccaa gaagtcgaat gctacgccga ttcgagccgc aacgactata
      601 cgcgtgccgc cgaactggtc aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt
      661 tggaacaggc agccgcatcc ggcttgttgg acggcaacga cgcctggagg cgcgtgcgcg
      721 gactgctggc cggccgccaa accacagacg cacgcaacct tgccgccgca ttgggcagcc
      781 cgtttgacgg cggtacacaa ggttcgcgcg aatatgccct gttgaacgtc atcggcaaag
      841 aagcacgcaa atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc
      901 tcgaacaacg cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa agcctcaatg
      961 tgcctgccgc cttggactat tacggcaagg ttgccgaccg ccgccaactg accgacgacc
     1021 aaatcgagtg gtacgcccgc gccgccttgc gcgcccgacg ttgggacgag ctggcttccg
     1081 taatctcgca tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac
     1141 gcagtcgcgc cgcaatgggc aacacgcaag aggcggaaaa actttacaaa caagcggcag
     1201 cgacgggcag gaatttttat gcggtgctgg cgggggaaga gttggggcgc aaaatcgata
     1261 cgcgcaacaa tgtgcccgat gccggcaaaa acagcgtcct ccgtatggcg gaagacggtg
     1321 cagtcaaacg cgcactggta ttgttccaaa acagccgaac cgccggcgat gcgaaaatgc
     1381 gtcgtcaggc tcaggcggaa tggcgttttg ccacacgcgg ctttgacgaa gacaagctgc
     1441 tgaccgccgc gcaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg
     1501 aacgcaccga ccgcaaactc aactacacct tgcgctatat ttcgccgttt aaagacacgg
     1561 taatccgcca cgcgcaaaat gttaatgtcg atccggcttg ggtttatggg ctgattcgtc
     1621 aggaaagccg cttcgttata ggcgcgcaat cccgcgtagg cgcgcagggg ctgatgcagg
     1681 ttatgcctgc caccgcgcgc gaaatcgctg gcaaaatcgg tatggatgcc gcccagctct
     1741 acaccgccga cggcaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc
     1801 tgcaaaacaa cgaagtcctc gccaccgcag gctataacgc cggtccaggc agggcgcgcc
     1861 gatggcaggc ggatatcccc attgagggcg cggtatatgc cgaaaccatc ccgttttccg
     1921 aaacgcgaga ctatgtcaaa aaagtgatga ccaatgccgc ctactacgcc gccctcttcg
     1981 gcgcgccgca catcccgctc aaacagcgta tgggcaccgt tcccgcccgc tgacgtaccg
     2041 atgccgtctg aaacccgccc ggtcttttag acggcatttt tatcccgaac ggcattgacg
     2101 gcgaaccata aatataagac aatccgaaaa ttgtttttcc tgctttttca agcagcttga
     2161 cacggcacaa gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc
     2221 gaaaggcaca gcgatacgga aaacctacaa caccgtctgc cctacccttt ccaccgatat
     2281 gatgggcaga tgcaacaaca gaatttatta aaggaaataa aatgcctgca atccgcgtaa
     2341 aagaaaacga accctttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg
     2401 gcctgctgac cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac
     2461 gcaaaaaagc ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa cagctgccgc
     2521 ccaaaatgta ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca
     2581 ccgcaagacc tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtctc
     2641 catccgacat cccagcgagg acattatgag cctgaaaatc cgccttaccg aagacatgaa
     2701 aaccgcgatg cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc
     2761 cgtcaaacag tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat
     2821 cctgaccaaa atggtcaaac agcgcaaaga cagcgcgaaa atctacactg aagccggccg
     2881 tcaggatttg gcagacaaag aaaacgccga aatcgaggta ctgcaccgct acctgccgca
     2941 aatgctctcc gccggcgaaa tccgtactgc ggtcgatgcc gccgttgccg aaaccggcgc
     3001 ggcaggtatg gcggatatgg gcaaagtgat gggcgtattg aaaacccgcc tcgccggcaa
     3061 agccgatatg ggcgaagtca acaaaattct gaaagccgta ctgaccgcct gattgtatga
     3121 atataaggaa aaatgccgtc tgaagcccgt atcgcaggtt cagacggcat tttcaatgtc
     3181 ccaatatcga atcggcaggg gcaacacggt ttcgatacac cgaaacgggt tttgccgata
     3241 aacggattcc gtttgcgccc catcggacaa aatgccgtct gaaacacgat tccgttcaga
     3301 cggcatagat ttatttgacc aatttcaagc cttttttggc gggccggggc gcggtttcgg
     3361 cagaggtgtt ttcaggcggc gtgtcggggc gatacgcttc caattcaaac cccatacctt
     3421 cgccggtctc ccgtgcgaaa aggctgagga cgtgtccgac aggtatccat atatcgtgcg
     3481 cctgtccgct gaaacgggcg gaaaaactga tccaatcgtt gtcgatttga aggttttgcg
     3541 tggcggtcgc gccgatgttg agcataattt cgttgtcgcg gacatactgc atagggacgc
     3601 gcgtgtgttc gttgacccag acaaggatgt gcggtgtgag gctgttgtcg ctgcaccatt
     3661 cgcagagggc gcggaggatg tagggtttgg tggaagtggg cataatggtt ccgtgttgta
     3721 cgccaaaata ggaaaatgcc tgcaaaacgg cgggttttgc aagcatttcg gacttatttg
     3781 cgcatggctt tttcggcggg tgtcagtgct tcgataaagg cttcgcgctg gaagatgcgc
     3841 tcggcgtatt tgagcagcgg cgcggcactt ttgcccagtt tgacatcgta gtggtcgagc
     3901 cgccacagca gcggagcaag ggcgacatca atcatagaaa aatcttcgcc gaggatgtat
     3961 ttgcttttgc tgaacgaagg ggcgagcatg gtcagaccgt tgccgatggc ttcgcgcgct
     4021 tttgcctgct ccttattggt agcggcgggg ttttccaaca cttggacgtg gttgaacaat
     4081 tctttttcca tacggtacag caccagccgg cccctgccgc gcataacggg atcgccgggc
     4141 ataagctgcg gatgagggaa gcgttcgtcg atgtattcgt tgatgatgtt ggactcgtgc
     4201 agcaccaaat cgcgctcgac cagtacgggg acctggttgt acgggttcat gacggcgagg
     4261 tcttcgggtt tgttgtaaat atcgacgtct ttgatttcaa aatccatacc tttttcgtac
     4321 aaaacgaagc ggcagcggtg gctgaagggg caggtaatgc cggaatagag ggtcatcata
     4381 ataattgtcg ctcctgtgtg atgcctacaa aacggc
//
LOCUS       DAWFBO010000152         4393 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFBO010000152 DAWFBO010000000
VERSION     DAWFBO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.152"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(26..349)
                     /locus_tag="WGO87_001750"
     CDS             complement(26..349)
                     /locus_tag="WGO87_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0393075.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(337..576)
                     /locus_tag="WGO87_001751"
     CDS             complement(337..576)
                     /locus_tag="WGO87_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0393076.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(699..1496)
                     /gene="truA"
                     /locus_tag="WGO87_001752"
     CDS             complement(699..1496)
                     /gene="truA"
                     /locus_tag="WGO87_001752"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0393077.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALENALAQI
                     AGEAVSTIVAGRTDTGVHATAQVVHFDTTAARPQQAWIRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
     gene            complement(2292..2984)
                     /locus_tag="WGO87_001753"
     CDS             complement(2292..2984)
                     /locus_tag="WGO87_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0393078.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            complement(2981..3724)
                     /locus_tag="WGO87_001754"
     CDS             complement(2981..3724)
                     /locus_tag="WGO87_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0393079.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            complement(3753..4316)
                     /gene="gmhB"
                     /locus_tag="WGO87_001755"
     CDS             complement(3753..4316)
                     /gene="gmhB"
                     /locus_tag="WGO87_001755"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0393080.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
BASE COUNT          856 a         1050 c         1403 g         1084 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatctc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaatttccaa gatgtttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatgg gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagagact acgacacaag gacgtgtctt tttgatttcg cttcctacgg
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaatgctc ctgctcggtt tcgacaagca ttgcggcagc
      421 ttctgcccat cccctgcgaa cggtaggaca gcttaaaatg atattgccct tttcaactgt
      481 aacagccaag ctgtctactg cccctatttg acccaataat gatttgggca gaatcacgcc
      541 ttgcgagttt cccattttgc gtatgttgag aatcatatac gtaccatatt caccagttta
      601 tgtaataaca atgttagtac cttgatgagg tagtgtcaac attgaaaaag attgcctgac
      661 agtttgtccg atttcaaaat ctccgcgaca agaatgtttt aaagccattc ggggatttgg
      721 gggcggatga tgccgtatgc ctcgggatag tcgacgccgg tcaggtaaag cccgtcgggc
      781 ataaaggtcg gtggggcttt gaggcgactg cgttcttgaa tcagtgcggc gaagccttcg
      841 acgctgagtc tgccgctgcc gacatagacg agcgcgccca tgatgttgcg taccatgtgg
      901 tgcaaaaagg cgttgccgtg caaatcgagg cggacgagtc ctgcgctttg ggtaaggtcg
      961 gcgcggtaga tggttttgac gggggatttt gcttggcatt cggcggcgcg gaagctggag
     1021 aagtcttgtt cgccgatcaa ataggcggca gcccgacgca tcggttcgat gtcgagtttg
     1081 aggtgtgtcc agcctgccct gtttttgagc aggggggaac ggacgggggc ggattcgagc
     1141 aggtagcggt agtgccgtcc gtatgcgtca aatcgtgcat gaaattcggg ggcgacctgt
     1201 cgggcgtgca aaacggcaat gccttcgggc aggtgggcat ttacgccgcg tatccatgcc
     1261 tgttggggac gggcggcagt tgtgtcgaag tggacgactt gggcggtggc atgcacgccg
     1321 gtgtcggtcc tgccggcaac aatggtggaa accgcttccc ctgctatttg ggcaagtgcg
     1381 ttttccaatg ccgcctgaac ggtcggtacg ccgtcagcct gtttctgcca gccgtaaaag
     1441 cggctgccgt cataggatag ggttattgcc cagcgttgtt tttgtgcggt atccatcgga
     1501 tttgggattc ggataaatgt tcagactgca ttgtatcgca gattttgcag ggaaacggca
     1561 aacgcccagg gcgagcggcg ttgtttgggg agttgggggg tggatgcagt tgctacgaat
     1621 cgctatcctg tgaattcacc ctgtcaggag tgcccgaatc gtcattcccg cgcaggcggg
     1681 aatctaggtc tgtcggtgcg gaaacttatc gggtaaaacg gtttcttcag attttacgtt
     1741 ctggattccc actttcgtgg gaatgacggg atgtaggttc gtgggaatga cgcggtgcag
     1801 gttttcttaa ccccgcgttc tagattccca ctttcgtagg aatgacggcg gtagatttgg
     1861 cagatgcggc ggattcggca ggtctcaacc catcctacaa tccaccctga ccgcctttta
     1921 cgaatccgcc gccatcctgg gaaacgcaaa aaaatgccgt ccgaaaacct ttcagacggc
     1981 attttcgcgg gcaaatcagt aaaagacttc gcgtcagctt aagcgtttca taccgcacgt
     2041 cggaccgggc gggtttcggg tttcggaaga gtggtgtgaa atgaaacgcg gcagacggcg
     2101 tgtgtgccgc catcctgccc cgaaccggcg gcaaagcgtc atcgcggttt gaatcgggcg
     2161 ggcgcgtgcc tgttccggca cggatgccgt ctgaaagcgg cggcagcggt atgtgtcgcc
     2221 ggctgtgatg ctgctttccg gatgtccgtt tgggaggagg tctgaattgg cggatgcctg
     2281 cccgggccgc gtcagcctaa ggcgaaaagt tccctgccgt ggattttgag ccactgtttc
     2341 gcttcgggcg tgtagggtgc gaagcggcgg gtcagtgccc agaacgcgcg gctgtggtcg
     2401 gcgtgggcga ggtggcagag ttcgtgtatg cagacataat cggcaacgta ttccggtgcg
     2461 ccgaccagcc gccggttgag gcgtatgcct gtggttttgc ggcacacgcc ccagaaggtt
     2521 ttggcagagg tcagcgagga gggggcgggg aacagttgtg tggtgcgggc gtggcgttcg
     2581 aggcggggaa tcaggtaact gtgcgcctgc cgttccaaaa agtcccgcag cagcgcaagc
     2641 tgtttttcgg gtgcgccttc gggaacacgg atttcagacg gcatcagcag gatttgcgtg
     2701 tcttgatggg cggtgagggc aagctgtctg ccgtggaaga ggatggattc gggcagccgg
     2761 ttttcggcag tttgcggcgg cggtgttttc gccagtgttt gccgcaggac ggcttcgttt
     2821 tcatacagcc agcggtttag agcggagacg gagaagcagg gtgggacgct gatgcggacg
     2881 gtatgtgtgc cggcggggcg gataatcagg tttttcttgg cacggcgctt gatttcgacg
     2941 gtcagttcca tgccgtctga aagggtgtgg acaaaggcgg tcatgcggtt tcagacggca
     3001 ttttggcggc aaacgggcct gcgccggaaa tgagcggctg ctgcgtttcg atgaggtgtt
     3061 cgcattttcc catcaattcg gcttcgctgc cgcttgcgtg cgggatggtc ggacagatga
     3121 cgacggtgat ttcccccgga tatttcagaa aggagttttt cggccaaaat tcgccgctgt
     3181 tgagggcgac ggggacgatg tccatctcaa acattttcgc catgcgcgcg ccgccgagtt
     3241 tgtatttgcc gcgttttccg ggcgcaaggc gcgtgccttc ggggaaaatg gtaatccaat
     3301 agccttcgtt tttgcgcgcc aacccctgtt ttatgagctg ctcgttggct tcgcggcggt
     3361 tgttgcggtc tatgcctatg gttttgacca gtttcaagcc ccagccgaaa aaggggattt
     3421 tgaacaactc gcgtttggca acgtaaacct gcggcggaaa aatgtcctga agggcgagcg
     3481 tttcccagcc gctttggtgt ttggcgcaga tgacggcggg gcggtcgggg atgttttccg
     3541 cgccgatgat gcggtatttg agcccgacga tgtgtttgag cgagaggttg aggattttga
     3601 cccagacccg cgccatcttg tgcgccccgt ctcggaaagg cgaggcgagc agcataaagg
     3661 gaaagaggaa aatcaggctg gaacagagta tcagccagta aatcaggttg cggatgatga
     3721 gcatggtttt gccttgtcgg aatgcggtat gttcagtcgg cttgcggtgc ggtgttttcc
     3781 tgcatgatgt attgtgagaa atcgagcagg gtatcgaaaa cctgtgtgtg ttcgggtaat
     3841 tcgtgtccgt gttgggagag cgtttttttg ccttttccgg tcagaaccag cgcgggtttt
     3901 ccgccgacgg catcgattgc ctgcaaatcg cgcaggctgt cgccgaccag ccaggtttcc
     3961 gaggcttggg cgttgaagcg tccgatgatg tcttcaatca tacccggttt gggcttgcgg
     4021 cagttgcagt tgtcggcatc ggtgtgcggg cagaaccaga tgccgttgat ttcgccgcct
     4081 gcctgacgga cgaggcggtg cattttggcg tgcatttcgg taaggttttg aacggtaaaa
     4141 tatttgcgcc cgatgccgga ttggttggtg gcaacggcga cggtgtagcc tgcctgcgtc
     4201 agaaatgcca ccgcatccat gctgccttcg acaggtatcc actcgtcaac ggatttgacg
     4261 aagtcgtcgc ggtcctgatt gatgacgccg tcgcggtcga gaatgatgag tttcatcgcg
     4321 tttcctttgg gttggggcag gtcgggggtg gcattatact gaaatatcgg cagtgatgcg
     4381 cctgtgccgc gtg
//
LOCUS       DAWFBO010000153         4343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFBO010000153 DAWFBO010000000
VERSION     DAWFBO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.153"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            142..1110
                     /locus_tag="WGO87_001756"
     CDS             142..1110
                     /locus_tag="WGO87_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0393081.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAYAV"
     gene            1100..2074
                     /locus_tag="WGO87_001757"
     CDS             1100..2074
                     /locus_tag="WGO87_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0393082.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     QLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVYLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVKHSVRLP
                     MTVCIGGILLVGGQTVFEHLLGMQAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2118..2744
                     /locus_tag="WGO87_001758"
     CDS             2118..2744
                     /locus_tag="WGO87_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393083.1"
                     /translation="MTQEHFPSFFNQAPTITVQDALAEFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRHLLSFGVGEINGTLTLRRKDNGKTVAVGLNAALQPFAPE
                     MRDIMPKAVSGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            2827..3585
                     /locus_tag="WGO87_001759"
     CDS             2827..3585
                     /locus_tag="WGO87_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0393084.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPNDIFTAANIKTLFDMDVDVLDYEGKKLVIHHI"
     gene            3877..>4343
                     /locus_tag="WGO87_001760"
     CDS             3877..>4343
                     /locus_tag="WGO87_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NadA"
                     /protein_id="HEZ0393085.1"
                     /translation="MKHFPSKVLTTAILATFCSGALAATNDDDVKKAATVAIAAAYNN
                     GQEINGFKAGETIYDIDEDGTITKKDATAADVEADDFKGLGLKKVVTNLTKTVNENKQ
                     NVDAKVKAAESEIEKLTTKLADTDAALADTDAALDATTNALNKLGENITTFTE"
BASE COUNT          966 a         1244 c         1115 g         1018 t
ORIGIN      
        1 gcgggaatgg cggcaagcgt ttttattgga acttgcccta tcctctgccc gatacggagg
       61 cttcaaacaa ggctttccgc tccgacggtt cggactgcct tgtttgaatc ttctacgcct
      121 taacgctttt cccttctgtt tatgactgcc aaaccttttt ccctcaacct gaccaacctc
      181 ctgctgctgg cggtgttgtt tgccgtcagc ctgtcggtcg gcgttgccga tttccgctgg
      241 tcggatgtgt tttcgctgtc ggacagccag caggttatgt tcatcagccg cctgccgcgc
      301 acgtttgcga ttgtgctgac gggcgcgtcg atggcggtgg cggggatgat tatgcagatt
      361 ctgatgcgta accgttttgt cgaaccttct atggcgggcg cgggtcagag tgcggctttg
      421 ggtttgcttc tgatgtccct gctgctgcct gccgcgccgc tgccggtcaa aatgtcggtt
      481 gccgccgttg ccgcgttaat cgggatgctg gtgtttatgc tgctgatccg ccgtctgccg
      541 ccgaccgcgc aactgatggt gccgctggtc gggattattt tcggcggcgt ggttgaggcg
      601 gtggcgacct ttatcgcgta tgaaaacgaa atgctgcaaa tgctcggcgt gtggcagcag
      661 ggcgattttt caggcgtact gctggggcgg tacgagctgc tttggattac gggcggtttg
      721 gcggtgtttg cctatctgat tgccgaccgg ctgacgattt tggggctggg cgagacggtc
      781 agcgtgaatt tgggtttgaa ccggacggcg gtgttgtggt cggggctgat tattgtggct
      841 ttgattacgt cgctggttat cgttacggtc ggcaatatcc cgtttatcgg gctggtcgtg
      901 ccgaacatca tcagccgcct gatgggcgac aagctgcgac aaagcctgcc tgcggtggcc
      961 ttgctgggcg catctttggt gttgctgtgc gatattatcg gacgcgtgat tgtgtttccg
     1021 tttgaaattc cggtctctac ggtttttggt gtattgggta cggctttgtt tttgtggctt
     1081 ttgttgagga aacccgccta tgccgtctga aaaaaatatc ggttttatgg caggaagcag
     1141 ccgcccgttg tgggtcgcct ttgcgctgtt gctggtttcc tgcgtcctgt ttatgacgct
     1201 caacgtcaaa ggcgattggg attttgtttt gcaactgcgg ctgaccaaac ttgccgcgct
     1261 gctgatggtc gcctatgcgg tcggcgtgtc cacgcaactc ttccaaacgc tgaccaataa
     1321 tccgattctg accccttcaa ttttgggttt cgattcgctg tatgtgtttt tgcagacctt
     1381 gctggtgttt acgttcggcg gcgtgggcta tgcttccctg ccgttgacgg gcaaattcgg
     1441 ctttgaactg gtcgtcatga tgggcggctc gctgctgctg ttctacacgc tcatcaaaca
     1501 gggcggacgc gatttgtcgc gcatgatttt aatcggcgtg attttcggga ttttgttccg
     1561 cagcctgtcg tcgctgcttt cgcgcatgat cgatcccgaa gaatttaccg ccgcgcaggc
     1621 gaatatgttt gccggattca ataccgtcca cagcgagctt ttgggcatag gcgcgctgat
     1681 tctgctcgtc agcgcggcgg tcgtttggcg cgaacgctac cgcttggacg tttacctttt
     1741 ggggcgtgac caagccgtca atttgggcat cagctacacg cgcaacacct tatggatact
     1801 gctttggatt gccgcattgg tggcgacggc gaccgccgtg gtcggccccg taagcttttt
     1861 cgggcttctc gccgcctcgc ttgccaacca cttttccccg tcggtcaaac attccgtccg
     1921 cctgccgatg acggtttgta tcggcggcat cctcttggtc ggcggacaga ccgtgttcga
     1981 acacctgctc ggtatgcagg cagtgttgag cgtagtagta gaatttgccg gcggactcgt
     2041 tttcctctat ctcgttttaa aacacaaaaa atgacggatg ccgtctgaac ggccgccgcc
     2101 ccgaaaggac aaaccatatg acacaagaac atttcccatc attcttcaac caagccccga
     2161 ccattaccgt ccaagacgca ttggccgaat tcctcggcgc ggccgaaaac ggcatcctca
     2221 cttaccgcta cgccgatgcc gtgcgcctgt gcggacattc ctgcccgacc gtcgcgggcg
     2281 cgtacctgat ggttatcaaa ggtctgaaag cactttacgg cgaagagctg cccgaacgcg
     2341 gcggcatcga agcctttatg cagggcgcgc gcgacgaagg cacggtcggc gtaaccgcgt
     2401 ccgtcgtcca actcctcacc ggcgcagccc ccgaaaccgg ctttggcggc atcggaatgc
     2461 agggacgctt tgcccgccgc cacctcttat cctttggtgt aggcgaaatc aacggcacac
     2521 tgaccctgcg ccgcaaagac aacggcaaaa ccgtcgccgt cggcctcaac gccgccctgc
     2581 aacccttcgc acccgaaatg cgcgacatca tgcccaaagc cgtcagcggc agcgcaagcg
     2641 cagaagaact cgaacgcttc ggacaactct ggcaggcacg cgttaaagca tttttaaccg
     2701 aatcggcgga cgacccgcag ttcgtcatcg tccgcgaagt gtgagcgttc agacggcatt
     2761 ccgaatttca aatgccgtct gaaccccgcc aaacaacaac aaacctacgc ccgacaagca
     2821 tccgccatga ttaccatccg caacgtcagc taccgcatcg gcacacgccc catcctcgac
     2881 aacgtcagcc tcgacatccc cgaaggcggc attaccgccc tcgtcggccc caacggtgcg
     2941 ggcaaatcca ccctgttttc ctttatggcg cggctgcgac cgcttgaaag cggcagcatc
     3001 gcctaccgag gcaaaaatct tgccgatacc cccaccgccg aactcgccaa aaccctgtcc
     3061 atcctcaccc aagaaaacag catcatgagc cgcatcaccg tgcgcgacct gctgatgttc
     3121 ggccgttacc cctaccatca aggcagaccg actgccgaat gccgccgtat cgttaacggt
     3181 gcaatcgaag aattccacct gcaagacctc tccgaccgct acctgaccga gctttccggc
     3241 ggccaacgcc aacgcgccat gattgcgatg gtgttctgcc aaagcaccga ctacgtcctt
     3301 ttggacgaac cgctgaacaa cctcgatatg tatcacgccc gctcgctcat gcaaatcctg
     3361 cgccggctga ccgacgaaca caagcgcacc accgtcgtcg tattgcacga catcaaccag
     3421 gcagcagcct acgccgacca cgtcgtcgcc atgaaaaacg gccaagtcgc catgcagggc
     3481 aaacccaacg atattttcac cgccgcaaac atcaaaaccc tattcgatat ggacgtcgac
     3541 gtcctcgatt acgaaggcaa aaaattggtt atccaccata tctaaatccg acaaaaaggc
     3601 cgtctgaaca ttcagacggc aacccatatc ctgacaaaat taagacacga caccggcaga
     3661 attgacatca gcataatatg cacatattaa cagatattaa tgccgaacta cctaactgca
     3721 agaattaaat aaataaataa ataaataaat tgcgacaatg tattgtatat atgcctcctt
     3781 tcatatatac tttaatatgt aaacaaactt ggtggggata aaatacttac aaaagatttc
     3841 cgccccattt tttatccact cacaaaggta atgagcatga aacactttcc atccaaagta
     3901 ctgaccacag ccatccttgc cactttctgt agcggcgcac tggcagccac aaacgacgac
     3961 gatgttaaaa aagctgccac tgtggccatt gctgctgcct acaacaatgg ccaagaaatc
     4021 aacggtttca aagctggaga gaccatctac gacattgatg aagacggcac aattaccaaa
     4081 aaagacgcaa ctgcagccga tgttgaagcc gacgacttta aaggtctggg tctgaaaaaa
     4141 gtcgtgacta acctgaccaa aaccgtcaat gaaaacaaac aaaacgtcga tgccaaagta
     4201 aaagctgcag aatctgaaat agaaaagtta acaaccaagt tagcagacac tgatgccgct
     4261 ttagcagata ctgatgccgc tctggatgca accaccaacg ccttgaataa attgggagaa
     4321 aatataacga catttactga aga
//
LOCUS       DAWFBO010000154         4320 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFBO010000154 DAWFBO010000000
VERSION     DAWFBO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.154"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..248)
                     /locus_tag="WGO87_001761"
     CDS             complement(<1..248)
                     /locus_tag="WGO87_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393086.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITR"
     gene            complement(245..391)
                     /locus_tag="WGO87_001762"
     CDS             complement(245..391)
                     /locus_tag="WGO87_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393087.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(408..746)
                     /gene="erpA"
                     /locus_tag="WGO87_001763"
     CDS             complement(408..746)
                     /gene="erpA"
                     /locus_tag="WGO87_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0393088.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(878..1564)
                     /locus_tag="WGO87_001764"
     CDS             complement(878..1564)
                     /locus_tag="WGO87_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0393089.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            1752..2024
                     /locus_tag="WGO87_001765"
     CDS             1752..2024
                     /locus_tag="WGO87_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393090.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2232..4160
                     /gene="dnaK"
                     /locus_tag="WGO87_001766"
     CDS             2232..4160
                     /gene="dnaK"
                     /locus_tag="WGO87_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0393091.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1324 a         1141 c          865 g          990 t
ORIGIN      
        1 ctccgggtaa taatatccaa acccaaatag ctgaaataat cgtgcaactc ttcccgtttc
       61 gcccaagtgc atttttcctc aaccagcact tgaggtttgg aatcgagcga ttcgatcaat
      121 ccttccgcct tttcctcgcc gagattctcc ctgaggcact cccggataag cccgcgatcc
      181 acttggggcg ggtaggaaac atatacatcg tacaaatcat ttcggtaatc tttactcatc
      241 tcaatcataa gggaacaaat acgcctgata ccgttccgcc cgttcctttc gggaaacccg
      301 attcatcttc aaacaatcgg attatatgtg aattcatccc gataatatat tttccaaaag
      361 taaaaatgcc gtctgaacgt tcagacggca tcacacaaac caagcggtca tacggaaaac
      421 gacgatccgc aaccgcaggt tgtttccgca ttcggattgc ggatgacgaa ttgcgaaccc
      481 tgcaaacttt ccgtatagtc gatttccgca ccgaccagat attgatagct catcggatcg
      541 accaaaaaga ccaaaccgtt tttctcaatt tcaaaatcgt cgtcgttttt gatttcgtca
      601 aaagtaaatc cgtactggaa acccgaacag ccgccgccat tgacaaaaac ccgcaatttc
      661 aaatcgggat tgttttcttc ggcaatcaaa tcggcaactt tggcacagca gctgtcagta
      721 aaaataatag ggctttcatc cgacatggtg ttattcctta atataatatt ttctgcattc
      781 tctctcaaac cccctttaaa ggcaagcagg cttcgccttt gaaaaaggaa ttgttccaat
      841 agataagttt gaccgcctgc aatttcaagc cccaaactca aatcgtcctg ccgaaccact
      901 cgacacgccc gataatctcc acatcatcgg tcaaatcgtt caaattgatt tcaaaagcag
      961 gataagcctc gtttgcagaa atcacattga taatcccgcc cggtacaatc tgtaaacgtt
     1021 taaccagcag attttcatta atccgcaaca catacagacc gtccctcggc gtattttcac
     1081 catgattgac caaaatcgaa tcgccgtcat tcaaaacccc ctccatcgaa tcccccttga
     1141 cggaaattac agacaggttt ttcgtatcgc gggtaacata attctcaatc caatgccgtc
     1201 tgaacgccat tgtaaatacc ggttcctcat gatcgacaaa ctgcccgtat cccgcagccg
     1261 cccgaatatc atatctcggc acaaagacaa actcgtccgt atcgacttca tttcccaagg
     1321 tatcgtaagc aagggatttt tttggggctt catccggaaa cggattaccc tccccggtca
     1381 gcagccaatc gatactacac cccttcaact gcttgatttt tttcaatgtt tcagactttg
     1441 gcagaccgcc ttcattccat atcctgctga agcccgcaat cgtcatttca atatcggatg
     1501 cgatttttgc ctgcctcgct tcgcttttcc ataaaaaaac cagtctgtct ttaaaggttt
     1561 ccataaatat ccttccactc cccttcattc aatcatctaa agtaaacatg aaatatattt
     1621 taccttagct atccaaaaaa cggaacaatt atgataaact aaatttaaat tatgattttt
     1681 cttacttaat acataatttt tcagattttt ccataggttt aatcctgaat tgacagaaaa
     1741 aggatagaaa aatgaagaaa accggcaaat atcttatcta tactgcggca tttaccgcat
     1801 tctgctttgc cttccaagaa aaccgttctg aagccaaaca gcccgacatc actttatccg
     1861 catccctgtg cgaacaattc aacatactga acgccaaaga tatggatgca gaacaagtct
     1921 ccctttccaa agaatgcgac atcatcgagt cttcacacga ctgggaaaaa gagtacggca
     1981 acttgaacga acaggaaatg ctcgccggca tcgtctacga ataaacctgc ctgccatttg
     2041 aaatattatg cttgaatgca ttggagccaa atgtattaaa tcaaatataa aaccaatata
     2101 ttcataaagt tatatactta tagccatgct gtagcttgaa acagcccgcc gcccgcccta
     2161 tttacagccc atcgggacaa aatgttccaa catatttctc aaaataagca aaatcaaata
     2221 ggattatcca catggcaaaa gtaatcggta tcgacttagg tacaaccaac tcttgtttgg
     2281 ccatttccga aaacggtcaa accaaagtta tcgaaaacgc agaaggcgca cgcaccacgc
     2341 cgtccgttat cgcttatttg gacggcggcg aaatcctcgt cggcgcgcct gccaaacgcc
     2401 aagcggtaac caacgccaaa aacactattt acgccgccaa acgtttgatc ggccacaaat
     2461 ttgaagacaa agaagtccaa cgcgacatcg aatctatgcc tttcgaaatc atcaaagcca
     2521 acaacggcga cgcatgggta aaagcacaag gcaaagagct gtctcctcct caaatttccg
     2581 cagaagtcct gcgtaaaatg aaagaagccg ccgaagctta cttgggcgaa aaagtaaccg
     2641 aagccgtgat taccgttccg gcctacttca acgacagcca acgtcaagcc accaaagacg
     2701 caggccgtat cgccggtttg gacgtaaaac gcatcatcaa cgagccgacc gcagccgctt
     2761 tggcattcgg tatggacaaa ggcgacaaca aagaccgcaa agtagccgta tatgacttgg
     2821 gcggcggtac tttcgatatt tccatcatcg aaatcgccaa cctcgacggc gacaaacaat
     2881 ttgaagtatt ggctaccaac ggcgatactt tcttgggcgg tgaagacttc gaccaacgct
     2941 tgatcgacta catcattgac gagtttaaaa aagaacaagg cattgatttg aaacaagacg
     3001 tcatggctct gcaacgtctg aaagaagctg ccgaaaaagc caaaatcgaa ttgtccagcg
     3061 gccagcaaac cgaaatcaac ctgccgtaca tcaccatgga cgcaaccggc ccgaaacact
     3121 tggcgatgaa aattacccgc gccaaattcg aaagcctggt tgaagacctg attgcccgct
     3181 ctatcgagcc ttgccgcacc gcattgaaag atgccggtct gagcactggc gacatcgacg
     3241 acgtgatttt ggtcggtggt caaagccgta tgccgaaagt acaagaagcc gttaaagact
     3301 tcttcggcaa agagccgcgc aaagacgtga accctgacga agctgtcgcc gtaggcgcag
     3361 cgattcaagg cgaagtgtta agcggcggcc gcagcgacgt attgctgctg gacgtaaccc
     3421 ctctgtcctt gggtatcgaa accatgggcg gcgtgatgac caagctgatt caaaagaaca
     3481 ccaccatccc gaccaaagcg tcgcaagtgt tctctaccgc cgaagacaac caaagcgcag
     3541 taaccatcca cgtactgcaa ggcgaacgcg aacgcgcttc tgccaacaaa tctttgggtc
     3601 agttcaactt gggcgacatc gcacccgcac cgcgcggtat gccgcaaatc gaagtcacct
     3661 tcgatattga cgccaacggt atcctgcacg tttccgccaa agacaaaggc actggcaaag
     3721 cagccaacat caccatccaa ggttcttcag gtttgagcga agaagaaatc gaacgcatgg
     3781 tgaaagatgc cgaagccaat gccgaggaag ataaaaaact gactgaattg gtcgcttccc
     3841 gcaaccaagc cgaagccctg attcactctg tgaaaaaatc tttggccgac tacggcgaca
     3901 aactcgacgc agccgagaaa gaaaaaatcg aagccgcgct gaaagaagcc gaagaagcag
     3961 ttaaaggcga cgacaaagcc gctatcgatg ccaaaaccga ggcgctgggc gcagccagcc
     4021 aaaaactggg ggaaatggtt tacgctcaag cacaagctga agcccaagca ggcgaaagcg
     4081 aacaagccaa tgcttctgca aagaaagacg atgatgtcgt agatgccgac tttgaagaag
     4141 taaaagacga caaaaaataa ttaataccgt ctgaaaaaag cgcgaaccgt ttgattcgcg
     4201 cttttttcaa ttgagataaa agaccatagc ataacagagg ttccaagttc atctaccgta
     4261 atattcccaa acccaagttc caactgttgt atcggttctc tggaaatttt ttatatagtg
//
LOCUS       DAWFBO010000155         4276 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFBO010000155 DAWFBO010000000
VERSION     DAWFBO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.155"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            539..2266
                     /gene="ilvB"
                     /locus_tag="WGO87_001767"
     CDS             539..2266
                     /gene="ilvB"
                     /locus_tag="WGO87_001767"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0393092.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDSLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            2277..2768
                     /gene="ilvN"
                     /locus_tag="WGO87_001768"
     CDS             2277..2768
                     /gene="ilvN"
                     /locus_tag="WGO87_001768"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0393093.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2835..3128
                     /locus_tag="WGO87_001769"
     CDS             2835..3128
                     /locus_tag="WGO87_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0393094.1"
                     /translation="MSNIKIVALVTVKPEYTETLVAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3223..4236
                     /gene="ilvC"
                     /locus_tag="WGO87_001770"
     CDS             3223..4236
                     /gene="ilvC"
                     /locus_tag="WGO87_001770"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0393095.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRTDLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1117 a         1022 c         1072 g         1065 t
ORIGIN      
        1 aatcaatata ttaagtcgtt gttagccctc ttggttctgt caatgccgtc caatacagtc
       61 cggtgcaatg agccattttc aggaagtatg gttgggaata tttttgatat atcaaaaata
      121 aaatcaagtt caatcaatat cccgagtctt tattgggatg aaaccctgta aattttacaa
      181 ttcttgatat cgaagaaggg tttttcacat ctgcccctcc tacacaaact gtcaacaatt
      241 tccatctttc aaaaaaggta ctcctgattt tattctttta aaatcaatca ataatttctc
      301 tgctttcaaa aaatattaaa agatgaaaga tttttcttga ttgaactgtc ggtttgcgtt
      361 atcgtttcta ctctttttca caaactctgt gttcctttcc aattgattgg ataatccgtc
      421 tgttgccgtg ttcccccaat gcgcgtaccc taaatcgctg cttggtggaa ttgcattaca
      481 ggtgctgtgg caaagcggtt tgtccctatt ggtttgaaac cgtataaaag aggtcgttat
      541 gcagttatca ggcgcgcaaa tcatagtgca gagtctcaaa gccgaaggtg tcgagtacgt
      601 tttcggttat cccggcggtg cggttatcga aatctacgat gcccttttcc aactcaataa
      661 attcaagcac attctgaccc gtcacgagca ggcggcagta cacgcggcag atgcgtatgc
      721 gcgcgtcagc ggtaaggtgg gcgtggcatt ggttacatcc ggcccgggcg ttaccaatgc
      781 actgaccggt attgctactg cctatacgga ttcgattccg atggtggtca tcagcgggca
      841 ggtaggcaat tccctgattg gtacggatgc gttccaagaa gttgatacgg tgggtattac
      901 ccgtccgtgc gtcaaacaca atttcctggt tacggacatc aatgagttgg cggaaaccat
      961 taaaaaggcg ttccaaattg ccgcaagcgg ccgaccgggg cccgtggtgg ttgatgtccc
     1021 gaaagatgtt acgcaggcga tggcgaaatt cagctatcct caggaagaca tttttatccg
     1081 ttcgtatcaa ccggttgttc aagggcatat cggtcagatt aaaaaggccg tgcagatgtt
     1141 ggcatctgcc aaacgtccgg tcgtctattt tggcggcggc gtggtgttgg gtaatgcttc
     1201 tgaagagctg acccgatttg tccgaatgac gggtgctccg tgcacgggta cgctgatggg
     1261 cttgggcgct tatccttccg gcgaccgcca attcctcggt atgctcggta tgcacggtac
     1321 ttacgaggca aaccttgcca tgcagaatgc ggatgtcgtt cttgccgtag gtgcgcgttt
     1381 tgacgaccgt gtggtttccg taccgtccaa atttttcgag aaggcgaaga aggttatcca
     1441 tatcgatgtc gatccttcca gcatcgccaa acgcgtgaag gcggacattc cgattgtcgg
     1501 cgacgtgaaa aacgttttgt ccgagatggt tgcgctgtgg caaaaacaag agtccgtgcc
     1561 gtctgaagat gctttgggca aatggtggaa aaccatagag gaatggcgtt cccgcgattg
     1621 cttgtggttt gacaacggca gcgaaattat caagccacaa tatgtgattc agaagcttgc
     1681 cgagattacc ggcaattcgg caatcatcac atcggatgta gggcagcatc aaatgtttgc
     1741 ggctcaatat tatcccttcg aacgtccgcg ccaatggctc aactccggcg gtttgggtac
     1801 gatgggcgtg ggtctgcctt atgcgattgg tgcaaaactt gccgccccgg atcaagacgt
     1861 attctgtatc accggcgacg gctcgatcca gatgaacatc caagagctgt ccacctgttt
     1921 ccaatatcgg attccggtta acgtcattac actgaacaac ggttatctcg gtatggtacg
     1981 ccagtggcag gaaatatatt acggcggacg agagtcggaa acctatttcg attctttgcc
     2041 cgatttcgtc aaacttgccg aggcatacgg ccatatcggt atccgcgtgg acaagaagtc
     2101 tgatgtggaa ggtgccttgt tggaagcatt gaaccaaaaa gacagtctgg tgtttatcga
     2161 cttcctgacc gaccagaaac agaatgtgat gcctatggtt ggcaacggca aaggtttgga
     2221 cgaaatggta cttccgccgc atatgcgtgc ggacggaaag gcgtaaggag agacaaatgc
     2281 gacatatctt atctgttctg atcgaaaacg aatcaggtgc gatgagccgc gtggtcggtt
     2341 tgttctctgc acgcgattac aatatcgatt ctttggcggt tgcgccgacc gaagacaaaa
     2401 ccctgtcccg tatgaccatc gttacacacg gcgacgagca ggttatcgaa caaatcacca
     2461 agcaactcaa taaattgatt gaagtgatta aagtggtcga tttgaatgaa agccgttttg
     2521 tcgaacgcga actgatgttg gtaaaagtcc gtgccgccgg caaagaccgc gacgaatttt
     2581 tacgcttgac cgaaatctac cggggcagca tcatcgacgt aaccgaccgc agctatacga
     2641 ttgaaattac cggctcgacc gacaagctgg attccttttt ggaaacggtc ggacgcgccc
     2701 aaattttgga aaccgtacgt acaggcgcgg ccggcatcgg tcgcggtgag cgtattttga
     2761 aaatttaacg ccgtaacctt tcagacggca cggtatttga atgccgtctg aaaaacgaac
     2821 ggcaggagag atttatgtcg aacattaaaa ttgtcgcact ggttaccgtc aaaccggaat
     2881 acacagaaac actggtagca cagtttaaag aactggtcaa agccagccgt gcagaagagg
     2941 gcaacatcag ctacgatctc catcaggaaa tcggcaaacc gaaccgtttt gttttcgtgg
     3001 aaaattggaa atcccaagca gctattgacg aacataacgc cagcgcgcat ttccaagcct
     3061 tcgtccagtc cgtcgacggc aaaaccgaag cgttggaaat cgtattgatg aatgaagttg
     3121 ccgattaagc ggtacactct gtttaacccg tccgaagccg ctcaacattt taaggcttcg
     3181 acaactattt accttacaat catttacctt aaaggaaatc aaatgcaagt ctattacgat
     3241 aaagatgccg atctgtccct aatcaaaggc aaaaccgttg ccatcatcgg ttacggttcg
     3301 caaggtcatg cccatgccgc caacctgaaa gattcgggtg taaacgtggt gattggtctg
     3361 cgccaaggtt cttcttggaa aaaagccgaa gcagccggtc atgtcgtcaa aaccgttgct
     3421 gaagcgacca aagaagccga tgtcgttatg ctgctgctgc ctgacgaaac catgcctgcc
     3481 gtctatcacg ccgaagttac agccaatttg aaagaaggcg caacgctggc atttgcacac
     3541 ggcttcaacg tgcactacaa ccaaattgtt ccgcgtaccg acttggacgt gattatggtt
     3601 gctcccaaag gtccgggtca taccgtacgc agtgaataca aacgcggcgg cggcgtgcct
     3661 tctctgattg ccgtttacca agacaattcc ggcaaagcca aagacatcgc cctgtcttat
     3721 gcggctgcca acggcggcac caaaggcggt gtgattgaaa ccactttccg cgaagaaacc
     3781 gaaaccgatc tgttcggcga acaagccgta ttgtgcggcg gcgtggtcga gttgattaaa
     3841 gcaggttttg aaaccctgac cgaagccggt tacgcgcctg aaatggctta cttcgaatgt
     3901 ctgcacgaaa tgaaactgat tgttgacctg attttcgaag gcggtattgc caatatgaac
     3961 tactccattt ccaacaatgc ggagtacggc gaatacgtta ccggccctga agtggtcaat
     4021 gcttccagca aagaagccat gcgcaatgcc ctgaaacgca tccaaaccgg cgaatacgca
     4081 aaaatgttta tccaagaggg taatgtcaac tatgcgtcta tgactgcccg ccgccgtctg
     4141 aatgccgacc accaagttga aaaagtcggc gcacaactgc gtgccatgat gccttggatt
     4201 actgccaaca aattggttga ccaagacaaa aactgattgt tttcaaacgg gactgcctac
     4261 acatcgtgta ggcagt
//
LOCUS       DAWFBO010000156         4161 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFBO010000156 DAWFBO010000000
VERSION     DAWFBO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.156"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(142..939)
                     /locus_tag="WGO87_001771"
     CDS             complement(142..939)
                     /locus_tag="WGO87_001771"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0393096.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1094..3943
                     /gene="uvrA"
                     /locus_tag="WGO87_001772"
     CDS             1094..3943
                     /gene="uvrA"
                     /locus_tag="WGO87_001772"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0393097.1"
                     /translation="MYKHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMESGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFASVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIACGSPEQVAKVKGSYTGKYLKVVLR"
     gene            3977..>4161
                     /locus_tag="WGO87_001773"
     CDS             3977..>4161
                     /locus_tag="WGO87_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393098.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVY"
BASE COUNT         1077 a         1226 c         1007 g          851 t
ORIGIN      
        1 tgataaatca ccgtaacgtt aaatttccag attcccattt tcgtgaacat aacggtgtga
       61 acattagcaa aaattcaaaa aaccaaactt cctcattcgt gtgaatgatg gtactgatat
      121 tttctatttc agttcgcaaa attaatcttt caacacatct tgcacagccg cctgaatctt
      181 agccgccgct gcctcgattt gccgttgttc ggcagatggg tttgccgctg tttcaaccgg
      241 agcagtttgt aaagcaggct cagactcagg ctcggattcg atttgcggcg cagtcagcgg
      301 caaacgcgcc aatacagtgc taacgccagt tactttatcg ccaatcgcca cttgcgcctg
      361 cgcatcgaca ggcagataca tatccacgcg cgaaccgaag cggataaagc cataacgttc
      421 gccgcgggac agtttcgcac ctgcttgggt gtagcacaaa atacggcgcg ccaccaaacc
      481 ggccacttga acaaaagtaa tttcacgacc ggaagccgta gtcgccaaca ccgcattacg
      541 ttcgttttcc gtgctggctt tgtccaaatc cgcattcacg aatttgcctt tgttatagac
      601 cactttcgtt accgtacaat cggcaggcga tttttgcgaa tgcacgttga acacgttcat
      661 aaaaatactg attttcaaag catcgacatc acgatacgga tcgcgtgcgc gctcgaccac
      721 cacgatacgg ccgtcaaccg gactcaacac tgcttcagga ttttgcggaa tctcacgcgc
      781 agggtcgcgg aaaaactgca gggcaaatac ggtaaacacc caaaacggca aagaccacca
      841 gccgcaacac atcgacacca gcaggctcaa agccaaaccg ccgccaataa tcggccagcc
      901 ctcacgggcg ataatcgggt gggggtaaag acggttcatt tgtcatcctt ttgttcaagc
      961 gggttaaaat tgccgccatt ataacggaat ccgccgcgtt ccgccgacaa atgccgtctg
     1021 aacgcggctt cagacggcat ccgagaaagt gctacaatac cagttttcct ttatttttca
     1081 ggcggcatcc attatgtaca agcatcatcc ccaacattcg cacgacaacg acaccatccg
     1141 catccgcggc gcgcgcacgc ataatttgaa aaacgtcgat ttggacattc cgcgccacaa
     1201 gctcgtagtg gtaacaggct tatcgggcag cggcaaatcg tcgctggcat ttgatacttt
     1261 atatgccgaa ggtcaacgcc gttatgtcga gagcctttcc gcctatgcgc ggcagttttt
     1321 gcagatgatg gacaaacccg atgtcgattt gattgaaggt ctgtcgcccg cgatttccat
     1381 cgagcagaaa tccaccagcc acaatccgcg ttccaccgtc ggcacggtta cggaaatcca
     1441 cgactatctg cgccttttgt acgcacgcgt cggcacaccc tactgccccg aacacaagct
     1501 gccgctatcc agccaaaccg tatctcaaat ggttgatgcc gtcttaaaac tgccggaaga
     1561 cacgcgcgtg atgattctgg cgccggcagt tcgtgagcgt aagggcgagt ttgtcgactt
     1621 ctttgccgac ttgcaggcgc aaggctttgc ccgtgtgcgc gtggacggcg aagtttatca
     1681 gcttgacgaa gtgccgaagc tggagaaaaa catcaagcac aatatcgacg tggtcatcga
     1741 ccgcgtgaaa gtgaaggcgg acatcaagca gcggctggcg gaaagttttg aaaccgcact
     1801 gcgccacggc aacgagcgcg cgctggcgat ggaaatggaa agcggcgaag aacattggtt
     1861 ttccgcgcgt ttcgcctgtc ccgtatgttc atacagcctg cccgaattag agccgcgcct
     1921 gttttcgttc aacaacccga tgggctcctg cccaacttgc gacggcttgg gcaacaccaa
     1981 cttcttcgat cccgaaaaag tggtcgcgca tcccgaattg tcgctggcaa cgggcgcgat
     2041 tgacggctgg gataagcgca accagttcta tttccaaatg attcagtcgc tggcgcggca
     2101 ttacggtttc gatgtgcagg ctgcttggga aacgctgcct gaaaaagtca aaaaagtcgt
     2161 gctgcacggc tcgggcaagg aagtcattga tttcacctac ctatccgaac gcggcaccac
     2221 cttcaaccgc agccacgcct tcgaaggcat cattcccaat ctcgaacgcc gctaccgcga
     2281 aaccgacagc gaaaccgtgc gcgaaaaact gcgtgaatac cagaaccacc gcgcctgccc
     2341 gagctgcggt ggtgcacgtt tgcgcaaaga agcccgctac gtttacgtca gcggcgaacc
     2401 gctgcacgaa gtttccgcct ggccgctcac caaaacccac caattctttg aaacactgga
     2461 tttagacggc aacaaaaaac aaatcgccga aaaaatcctc aaagaaatca ccgaacgcct
     2521 cggcttcctg attaacgtcg ggctggatta cctgaattta agccgctccg ccgaaaccct
     2581 ctccggcggc gaagcccagc gcatccgcct cgccagccaa atcggcagcg gcctgaccgg
     2641 cgtgatgtat gtactggacg aaccctccat cggcctgcac cagcgtgaca atgaccgcct
     2701 gctggccacc ctcaaacgac tgcgcgattt gggcaacagc gtgattgtgg tcgaacacga
     2761 cgaagacgcc atccgcgaag ccgatttcgt ggtcgatatg ggccccggcg caggcgaaca
     2821 cggcggcaac gtactgattg ccgacacgcc cgaaaacgtc gcccaatgcg aaaactccgt
     2881 taccggacaa tacctcagcg gcaaaaaatc cattgccgtg ccgtctgaac gcacgcccgt
     2941 caatcccgac cggatgctcg tcctcaaagg cgcacgcggc aacaatctca aaaacgttac
     3001 ccttgagctg ccgctcggtt tgattacctg catcaccggc gtatccggca gcggcaaatc
     3061 caccctgatt aacgacaccc tcgccaaaat taccgcccgc gaactcaacc gcgcccaaga
     3121 agaacccgcc ccatacgacg acatccgcgg cctcgaacac ctcgacaaag tcatcaacgt
     3181 cgaccaatcc cccatcggcc gcaccccacg ctccaatccc gccacctaca ccggcctgtt
     3241 cacccccatc cgcgaactct tcgccagcgt acccctctcg cgtgaacgag gctacaacgt
     3301 cggcagattc tccttcaacg tcaaaggcgg ccgctgcgaa gcctgccaag gcgacggcgt
     3361 gattaaagtc gaaatgcact tcctgcccga cgtgtacgtc ccctgcgaag tctgccacgg
     3421 caaacgctac aaccgcgaaa cgctcgaaat ccaatacaaa ggcaaaaaca tcagccaagt
     3481 cctcgacatg accgtcgaag aagcccgcga atttttcgac gccgtcccca ccgtatcgcg
     3541 caaactgcaa accctgatgg acgtaggtct cggctacatc cgcctcggcc aatccgccac
     3601 caccctctcc ggcggcgaag cccagcgcgt caaactcgcc ttggaactct ccaaacgcga
     3661 caccggcaga acactctaca tcctcgacga acccaccacc ggcctacact tcgccgacat
     3721 cgccctgctg ctggaagtca taggtcgtct gaaaggcaaa ggcaactcga ttgtgattat
     3781 cgagcataat ctagacgtga ttaaaaccgc cgattggatt gtggacttag ggccggaagg
     3841 cggcgatggc gggggcagga ttattgcttg tggtagccct gagcaggtgg cgaaagttaa
     3901 ggggagttat actgggaagt atttgaaggt ggttttgagg taagtttaat tcaaaattaa
     3961 ataggagatt attaagatga aaccgttaaa aacattagaa tttggatttg tggatgctgc
     4021 aaactacaga agaagagaaa ataaagattt atttaaccga atatttgtaa aaggagaata
     4081 tttggatgaa ttatgtgaac caaatatttc gtttttaatc ggagaaaagg gaactggaaa
     4141 gacagcatat gctgtttatt t
//
LOCUS       DAWFBO010000157         4124 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFBO010000157 DAWFBO010000000
VERSION     DAWFBO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.157"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..142
                     /locus_tag="WGO87_001774"
     CDS             <1..142
                     /locus_tag="WGO87_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ0393099.1"
                     /translation="VGIGGGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRHI
                     QT"
     gene            272..1708
                     /locus_tag="WGO87_001775"
     CDS             272..1708
                     /locus_tag="WGO87_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0393100.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQQADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1719..2702
                     /locus_tag="WGO87_001776"
     CDS             1719..2702
                     /locus_tag="WGO87_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0393101.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2699..3892
                     /locus_tag="WGO87_001777"
     CDS             2699..3892
                     /locus_tag="WGO87_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0393102.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGVCSMKNWLNHGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
BASE COUNT          879 a         1345 c         1056 g          844 t
ORIGIN      
        1 cgtcggtatc ggcggcggcg cgctgctggg gcattgggtt acgcaatatg tcggcatttc
       61 ccgtatcggc atcgccggta tgcttgcggc ggcggccggg ctgtggctgt gcctgaactt
      121 gaaccgccat atccaaacct gaccgcgcga tgccgtctga agcccctccc gcccttcaga
      181 cggcattttg attgaagaaa tatccgcccc tgcttaaaat aacgggcttt gccgtgtttt
      241 aacgcctatt ttcttattcc aaggatggtt tatgccgctg ctgtctgtcg agttcgcact
      301 gttctttctc gccttcctgc cgatttactg gggcttggcg aaatacccgt ccgtccaaaa
      361 cctgctgctt ttggctgccg gtatgggctg gctctaccat atcggccccg tatttgcggc
      421 aatcgtcgtc ctttattcct cctgcgtgta ccttttgggc gaactgctcc gttccgatcg
      481 cgaaaatacg cgccgctttt ggctggggtg cggcattgcc gtctcgctga ccgtcttggg
      541 ctttttcaaa tatttcgatt ttttccgccc gctgattgca caatatgccg gacaaggcgg
      601 cgcaatcgac atcctgatgc cgctggggct ttcgtattac accttccagt caatcgccta
      661 tttggtttac tgcttccgtg cgccgcacgc cgcgcgtttc gagtggcacg aactgctgct
      721 gcacctgagt tttttcccca ccgttacctc cggcccgatt atccgcgccg ccgcattcaa
      781 aagcgcggac ggcgagcagg cgggcgcatt ggcgcaaatc cgtacccacc aaccacgctc
      841 acccgtccgc cccgcactcg ccgtttccct gattctgctg ggtattgcca aaaaatggtg
      901 gctggcgggg atgctcgcag aaaactgggt atcgcccgta tttgaaaatc ccgcccaatt
      961 cgacggctgg ggcgtattgg cgggcgtgta cggctatacc ttccaactct ttttagactt
     1021 ttccggatat tccgacttgg ttatcggaat ggcaatgctg ctgggcttcc ggctgcccaa
     1081 aaacttctcc gcaccgcttc gtgccgcgaa tatccgcgca ttttgggaca aatggcacat
     1141 cagcctttcc acctggatac gcgactacat ctacatcccc ttgggcggca gcaaaaaagg
     1201 ctttttgcgg acacagttca acctgatggc ggcaatggtg ctctcaggca tctggcacgg
     1261 ctacggctgg aacttcctca tttggggtgc actacacggc acggcgctgg tgctgctcaa
     1321 cacgggcgac cgctatttcg gacgcaacgc actatgccgt ctgaaatacc ttgcgccgct
     1381 ctcgtggttt gttaccttcc atttcgtctg cctgaccttc gtcgtcttca ataccgcaaa
     1441 ccccgacgat gccggcgcgg ttttcaatgc cctctttgcc aatgccaacg gctggaatgc
     1501 gccgcaacag gcggatatgc tgctgcttgc ctcgtttgca tccgtgatgc tgctctaccc
     1561 ttacctgcaa cgcgctttcg acggcgcggt caaaggtttg gaaaaaatcc cgatgtggct
     1621 gtggtttatc cccgtttccg ccgtcctgct gctgattatc gtattggccc cctcggggat
     1681 acccggcttt atttatgcca atttttaagg gtttggacat gaaaaacttt ctttcccttt
     1741 tcgcctccat actgatgtct gccctgattg ccgtgtggtt cagccaaaac cccatcaacg
     1801 cctactggca gcagacctac caccgcaaca gtccgctcga accgcttgcc gcctacggat
     1861 ggtggcggag cggtgtggcg ttgcaagaaa acgcctacgc cctttcagac ggcatcaaag
     1921 ccttcctgtc cggcgaaacg ccgccgacgg ctcaagacgg cggttcggca gatatgccgt
     1981 ctgaagccgc cgcatcagaa gccgtccctc aaaccggtga aacagaatgg aaacaagaca
     2041 ccgaagccgc cgccgtccgc agcggcgaca aagtcttttt tgccggcgac tcgctgatgc
     2101 agggcgttgc acccttcgtg caaaaaagcc tgaaacagca atacggcatc gaatccgtca
     2161 acctcagcaa acaaagcacg gggctgtcct acccctcatt cttcgactgg ccgaaaacga
     2221 ttgaagaaac cctgaaaaaa catcccgaaa tcagcgtgct cgccgtcttc ctcggcccga
     2281 acgacccgtg ggatttcccc gtcggcaaac gatacctcaa attcgcttcc gacgaatggg
     2341 cgcaagaata cctgaaacgc gtcgaccgca tccttgaagc cgcacacacg caccgcgtcc
     2401 aagtcgtctg gctcggcatc ccctatatga aaaaagccaa gctcgacggg cagatgcgct
     2461 acctcgacaa actgctttcg gaacacctgg aaggcaaaat catcctgatt cccaccgcgc
     2521 acaccctgag cggcggggaa gaccgctaca ccgactccgt caacgtcaac ggcaaacccg
     2581 tccgctaccg cagcaaggac ggcatacact ttaccgccga aggacaaaaa ctgctggcgg
     2641 caaaaataat ggaaaaaatc gtttttgaac caagtacgca accatcaagt acacagccat
     2701 gaaccccaaa cacctcatcg cattttccgc cctgttcgcc gccacgcagg cagaagccct
     2761 gcccgtcgcc tccgtcagcc tcgacaccgt taccgtttcc ccgtccgccc cctacaccga
     2821 tacaaacggg ctcttgaccg actacggcaa cgccgccgcc tcgccttgga tgaaaaaact
     2881 ccgatccgtc gcacaaggca gcggcgagac cttccgcatc ctgcaaatcg gcgactcgca
     2941 taccgccggc gacttcttta ccgacagcct gcgcaaacgc ctgcaaaaaa cttggggcga
     3001 cggcggcata ggctgggttt accccgccaa cgtcaaaggg cagcgcatgg cggccgtccg
     3061 gcacaacggt aactggcaaa gcctcaccag caggaacaat agcggagact tcccgctcgg
     3121 cggcatcctc gcccacaccg gcagcggcgg cagcatgacc ctgaccgcat cggacggcaa
     3181 gaccggcaaa cagcgcatct ccctgtttgc caaacccctg ctcgccgaac aaaccctgac
     3241 cgtcaacggc aacaccgtct ccgccaacgg cggcggctgg caggtactgg atacgggcgc
     3301 ggcactccca ctgaccatac agaccgaaat gccgtgggac atcggcttca tcaacatcga
     3361 aaatcccgcc ggcggcatta ccgtttccgc catgggcatc aacggcgcac aattgaccca
     3421 ttggtcgaaa tggcgtgccg accgtatgaa cgaccttgcc caaaccggcg ccgatttggt
     3481 tatcctttcc tacggcacca acgaagcctt cggcgacaac atcgacattg ccgacactga
     3541 acaaaaatgg ctggataccg tccgccaaat ccgcgacagc ctgcctgccg ccggcatcct
     3601 catcatcggc gcacccgaat ccctgaaaaa cacgctcggc gtatgcggca cacgccccgt
     3661 ccgcctgacc gaagtccaac agatgcagcg gcgcatcgcc cgtcaggggc agacgatgtt
     3721 ctggtcttgg caaaacgcga tgggcggcgt ttgcagcatg aaaaactggc tcaaccacgg
     3781 atgggccgcc aaagacggcg tacacttctc cgccaaaggc taccggcgcg cggcggaaat
     3841 gctcgccgac agcctcgaag aactcgtccg cgccgccgca atcaggcaat aatcggacag
     3901 ggggcggacg gtatttccgc aacaggggga tgccgtctga aacgcatacc ttcatattgc
     3961 ttcagacggc atagccaccc cgcgcacggt ttgccagatg caaccggcat tcgcctcagg
     4021 catcggaagg acgcaggcga acctccggca tacggcgcaa aggcggcgtt tgatatgccg
     4081 tctgaaggca aagatgataa actgccgcct tccgttttca gacg
//
LOCUS       DAWFBO010000158         4017 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFBO010000158 DAWFBO010000000
VERSION     DAWFBO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.158"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(1060..1449)
                     /locus_tag="WGO87_001778"
     CDS             complement(1060..1449)
                     /locus_tag="WGO87_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0393103.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILLVLQELEKQAAR"
     gene            complement(2022..2147)
                     /locus_tag="WGO87_001779"
                     /pseudo
     CDS             complement(2022..2147)
                     /locus_tag="WGO87_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(2193..3326)
                     /gene="carA"
                     /locus_tag="WGO87_001780"
     CDS             complement(2193..3326)
                     /gene="carA"
                     /locus_tag="WGO87_001780"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0393104.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYASGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKSFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT          906 a         1036 c         1050 g         1025 t
ORIGIN      
        1 aataacggcg tattgccttt gattataatt aaaaggaaat gcttcaatta ttctattttt
       61 gtttctcata tcattaagca aatactttaa attattgaaa ttaataggta ttgacatatt
      121 taaactcctg cgaatatgtt gcaataaacg tagcaatcgt aggttgggtt gaaaacccaa
      181 cacaaaccgc ttaattgtct aaaatgttgg gtttgctaac ctaacttgta tatcgaaacc
      241 cccgtattgt agcaagccgt agcccgtatg tagggtatgt gcggtacgta tgcacgcgtt
      301 ctccattttc cctgcaaccc caacccaccg tgtgcgtgcc ttgcggcaca caccctaccg
      361 atgggttcaa aggtcgtctg aaaaaacgct cgcggcattt gttccatctt ccttgcgata
      421 aaggctacct gaaaaattcc tgctgttgat atctcgggca ttctttctca aaccgcctgc
      481 aactgccaaa tctgctgcaa cctgccccct cccccgtggg ggagggttgg ggagagggca
      541 ttccccgaat ttcggcaacc tttcccaaca actcaaccac cgaaatacaa gccttgtggc
      601 ttgttgccct ctctctagct ctctcccacg ggagagagga cggagtggct gtagggctaa
      661 gggttctgca aggaaacagg ttgtgcaggt tgctttttgc ttttcagacg acctgaaatc
      721 aacggtaaat ccaagtcgct tttcaaaccg cctgcaaccg caaaatctgc tgaaacctac
      781 tccctccccc gtgggggagg gctggggaga gggcattctc cgaatttcgg caacctttcc
      841 taacactcta accgccaaga cacaagcctt gtggcttgtt gccctctctc taactctctc
      901 ccacgggaga gaggactggg cggttgttgg ggctaaggtt tctgtaaact aatcaggtta
      961 tgcaggctgt tttttgtttt tcagacggcc tttataaatg ctcaattcag tcggctttat
     1021 cctattattt tcaataatcc aatcaaaatc agccatctgc tatcgtgcag cttgcttttc
     1081 caattcttgc aacacgagca ggatttccgc cagtacatcg tttgtctgct gcaaaatttc
     1141 gtgattccaa aaacgcagca cggtaaagcc caggctgttg agatatgccg tccgcgcgtg
     1201 gtcgtatacg gcttgttccg cgtgctgccc tccgtctgct tcgacaatca acttgggcgt
     1261 tacgcacata aaatcaacaa tatagtttcc catcggctgc tggcggcgga atttatagcc
     1321 gttcagacga cctgcccgca ggtgctgcca caattttgct tccgccccgc tcatttcttg
     1381 gcgcatggct ttggcgcgtt ggcgcaagat ggggttgtcg gtggtcaaga gtttctcgtg
     1441 cgggttcatt ttgttgtcgt gctgtttgct gccctctctc taactctctc ccacggggag
     1501 agaggacggg gcggttgttg gggttaaagg ggctgtaagg aaaacaagtc gttcaggcca
     1561 ctttttactt ttcagacgac ctgcaatcaa cggttgtaaa tccaagttgc ttttcaaacc
     1621 gcctgtaacc gcaaaacttg ctgcaaccta ccccctcccc cgtgggggag ggctggggag
     1681 agggcattct ccgaatcgag gcaatctttc cctaattccc taactgcctc aatacaagcc
     1741 tttcggcttg ttgccctctc tctaacgctt tcccacggga gagaggacgg tgcggctgta
     1801 gtgattaagg gttttgcaag gaaaacaagg aaaacaagtt gtacaggctg ttttttattt
     1861 ttcaagcgac ctgaaatcaa cgcttgtaaa tccaagtcgc ttttcaaact gcctgcaacc
     1921 gcaaaatctg ctgcaaccta ccccctcccc cgtgggggag ggctggggag agggcatttt
     1981 ccaagttgcg gcaatctttc ccaaatccct tagtcttcta aaatacaagc cttacggttg
     2041 ttgccaaatg aaacttcttc catacccaaa acttgcgtgt aaaacgcgat ggttcggtca
     2101 atgtcggcaa cggtcagtac cagatggtcg agtgcgctaa tcttcatttc ccgctttctt
     2161 tttcaggcgg cggcagtgcc gtctgaaagc cattatgccc gttttgccgc tttcatattg
     2221 ccaatgaatt tgtcaaacaa atagccgaca tcttgcggac cggggctggc ttcggggtgg
     2281 ccttggaagc agaatacggg tttgtcggtc agctcgatgc cttgcaaagt gttgtcaaac
     2341 aaggatttgt gggtaatcct tgcgttggcg ggcagggtgt cggcatcgac ggcgaaaccg
     2401 tggttttggc tggtgatgac gactttgccg ctgtccaaat cttgcacggg gtggttcgcg
     2461 ccgtggtggc tgaagcgcat ttttaaggtt ttcgcgccga tagcgaggct gatgagctgg
     2521 tgtcccaagc agatgccgaa aatcggtttg ccgctttcca tcagtttttg cacggcttcg
     2581 atggcgtagg tgcaaggctc ggggtcgccg gggccgttgg acaggaacac gccgtcggga
     2641 ttgagtgcca acacgtcttg tgcgctcgtt tgggcgggga cgacggtcag gcggcagccg
     2701 cgcgaggcga gcatacgcag gatgttggtt ttcacgccga aatcgtaggc gacgacgtgg
     2761 taaggctgtt cgtcaggggt aacgaaactc ttgcccaatt cccattcgcc ttccgtccat
     2821 tcgtaagttt ccgtgcagga aacttctttt gccaagtctt tgccgaccat actgccgaac
     2881 gcggcgatga gttcttgcgc tttttcgatt gtggcatccg caccggtcag aatcgcgccg
     2941 ccttgcgcgc ctttttcgcg caacagcgtg gtcaggcggc gggtgtcgat gtcggcgatg
     3001 gcgacggttt ggttgcgtac caaatagtcg tgcaggcttt cggaggcgcg gaagttgctg
     3061 tgcaagagcg gcaggtcgcg gataatcagg ccggaggcat aaacgctgcg gctttcttca
     3121 tcttcggcgt tggtgccggt gttgccgatg tgggggtagg tgagggtaac gatttgtttg
     3181 cagtaggacg ggtcggtcag gatttcctga tagccggtca tcgaagtatt gaacacgact
     3241 tcgccggaag tcgaaccttc gtaaccgatt gatgtgccgt gaaatacgct gccgtcagcg
     3301 aggacgagga gggcgggggt gctcatgatg ggaatcctgt ttttaataaa attcttgaca
     3361 atgccgtctg aagggacttc ggcagggggc atccggtcgg aaaaatccgg tcaaaaaaaa
     3421 acacgccgcg caagatagac gcgtaacgtg cttttcgggc aagtgcctat accctgaaag
     3481 cagcatattt taagacgaaa catacccgat ttcaagcgca aacgcggcag gcgggctgat
     3541 atgttcgcgc gaaaacggaa atccgtggcg taaaaccgcc cgctctgcgc ccactatgcc
     3601 cgttacgcat ttccccacgt ccgtccggcg aaactatggg aatacccgaa tcgtcaggaa
     3661 tacctgaatc gtcaggaata cctgaatcgt caggaatacc cgaaccgtca ttcccgcgca
     3721 ggcgggaatc cagacctgtc ggcacagaaa cttatcgggt acaacggttt cttgagattt
     3781 tacgtcctag attcccgcct gcgcgggaat gacgcgtttc atttgccgcc ccctcccgaa
     3841 aaacgcaaaa aatgccgtcc gaaaaccttt cggacggcat tttcgcgtga accgtcattc
     3901 ccacgaaaac agaaaaccaa aatcagaaac ctaaaattcg tcattcccac gaaagtggga
     3961 atccaggact caggacctga gaaacctttt tacccgataa gttcccgtac cgaccgg
//
LOCUS       DAWFBO010000159         3988 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFBO010000159 DAWFBO010000000
VERSION     DAWFBO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.159"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WGO87_001781"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WGO87_001781"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0393105.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="WGO87_001782"
     CDS             1160..2290
                     /locus_tag="WGO87_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0393106.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTVEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2361..3212
                     /locus_tag="WGO87_001783"
     CDS             2361..3212
                     /locus_tag="WGO87_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0393107.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="WGO87_001784"
     CDS             complement(3295..3957)
                     /locus_tag="WGO87_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0393108.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEEFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          868 a         1175 c          983 g          962 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgatg tgcgtggctt tcacgccgtt gacggcggct tcgaccgcgg aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttca gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccgcgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatgatcgtc
      601 gcgtccgctt acgccgaccg cgtgcccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacgtgc ccgcccaaca ccatttcgac aatatccatc gtctctttgt cggtcacgcg
      721 cattccttgg acaaactcgc cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgataa cgggatggat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtact tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgctttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacgactccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatctggca gaccttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgacc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaat catacgccgc tgtccaacag cgtgtattac gcctatttcg aaccttactc
     1501 cgaagaacag catttaaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aaggccgcga catcaatctg ctgaccatcg gcaatcaggt
     1621 cgaaagcgat ctgaaaatct ggattaccgc ccgccagcat ccgggcgaaa ccatggcgga
     1681 atggtttgtc gaggggctgc tcggcaggct gctcgaccct caggatgcaa ccgcgcgcac
     1741 cctgctcgat cgcgccacgt tctatatcgt ccccaacatg aaccccgacg gttcggcact
     1801 gggcaacctt cgtaccaacg ccgccggtgc gaacctcaac cgcgaatggg aaaatccgac
     1861 tgtggaaaaa agcccggaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggcgtgga
     1921 tttgttcttg gacatccacg gcgacgaagg gctgcccttt atctttgttg caggaactga
     1981 aggcgtgcca aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2041 tttgaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgcccggtca
     2101 ggcaaacatg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gttggaaatg ccgttcaaag acaatgccaa cctgcccgac gacgatttcg gatggaacgg
     2221 tcaacggtcg ttgcgcttgg gcgaagccgc gttgtccgcc atcctcaaca tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcccgtt atgattaccc atccccaatt cgaccccgtc cttatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgctcg
     2521 acgacttcct gacatggggc attttgggcg taattttggg cgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggacacggt cgcaccgctc gttccgctgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagat gccgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt
     3001 tctctaaaaa acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa ttcgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gcatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggtttgttg atgactgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagcgc cgttttcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa attcttcatc atcggcggct tcaaaccaat cggtcggcac
     3601 aatatccaag ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccct
//
LOCUS       DAWFBO010000160         3984 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFBO010000160 DAWFBO010000000
VERSION     DAWFBO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.160"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            272..1789
                     /locus_tag="WGO87_001785"
     CDS             272..1789
                     /locus_tag="WGO87_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0393109.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALSQVWRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRKLHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPVPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQDGGS
                     QICSVSRAERATGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLSPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1848..3629)
                     /locus_tag="WGO87_001786"
     CDS             complement(1848..3629)
                     /locus_tag="WGO87_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0393110.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGIVATEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADILRAAFPCGSITGAPKKMSMQIIETLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENRQCALLDRHLCRLKAAAQALNLPLPDGCENQIKQYIARLPDGVFRVKALLASDG
                     ISLSRAVLNHLADKQRVIISPTILPAQNYLRHFKTTHRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLHTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          844 a          888 c         1294 g          958 t
ORIGIN      
        1 ctcgccttag ctcaaagaga acaattctct aaggtgttga agcaccaagt gaatcggttt
       61 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccgctat
      121 aaatgtgaaa tccgcccttt gaaaatcggg cggcggtttt ttgcctgccc gctttcagac
      181 ggcaaagccg gttttcacgg ttttctgcct gtttttcgga tggtttgacg tgtttcggcg
      241 gcgtgtttgc cggaaaggta aatgacagga tatgttgtat ttcagatacg gttttttggt
      301 tgtttggtgt gcggcaggtg tttctgccgc ctatggggcg gatgcgcctg caattttgga
      361 tgacaaggct ttgtcgcagg tgtggcggtc ggtgtcggat aaatgggcgg aatcggattg
      421 gaaagttgaa aatgatgccc cgcgcgtggt tgacggggat tttttgttgg cgcacccgaa
      481 aatgttggaa catagtttgc gcgacgcgct caacggcaat caggcggatt taatcgcttc
      541 gttggcggat ttgtatgcca agctgccgga ttatgatgca gttttgtacg gcagggcgcg
      601 ggctttgctg gcgaaattgg cgggaaggcc ggcggaggcg gtggcgcggt atcggaagct
      661 gcacggggaa aatgcggcag acgagcggat tttgctggat ttggcggcgg cggagtttga
      721 cgatttccgg ctgaagtcgg cagaaagaca ttttgcggag gcggcaaaat tggatttgcc
      781 ggtgccggtt ttggaaaatg tggggcgttt tcggaaaaaa acggaggggc tgacgggctg
      841 gcgtttttcg ggcggcatca gtccggcggt caatagaaat gccaataatg ccgcgccgca
      901 atattgccgg caagacggag gcagtcagat atgcagtgtc agccgggcgg agcgggcgac
      961 aggcttgaat tatgaaatcg aggcggaaaa gctgacggcg ttggcggaca atcattattt
     1021 gttgttccgt tccaatatcg gcggcacaag ctattatttc agtaaaaaat cggcttatga
     1081 cgacgggttc ggcagggcgt atttgggatg gcagtataaa aatgcgcggc agacggcggg
     1141 gattttgccg ttttatcagg tgcagttgtc gggcagcgac ggctttgatg cgaaaacaaa
     1201 acgggtaaac aaccgccgcc tgtcgccgta tatgctggcg cacggagtcg gcgtgcagct
     1261 gtcccatact taccgcccaa acccgggatg gcaattttcg gtcgcgctgg aacattaccg
     1321 ccaacgctac cgcgaacagg atagggcgga atacaataac ggcaggcagg acgggtttta
     1381 tgtttcgtcg gcaaaacgtt tgggcgaatc ggcaactgtg ttcggcggct ggcagtttgt
     1441 gcggtttgtg ccgaaacgtg aaacggtggg cggcgcggtc aataatgccg cctaccggcg
     1501 caacggtgtt tatgccggct gggcgcagga gtggcggcag ttgggcggtt tgaacagtcg
     1561 ggtttccgcg tcttatgccc gccgcaacta taagggcatt gcggctttct cgacagaggc
     1621 gcaacgcaac cgcgaatgga atgtctcgct ggctttgagc cacgacaagt tgtcgtacaa
     1681 aggtatcgtg ccggcgttga attatcgttt tggtaggacg gaaagtaatg tgccgtatgc
     1741 gaaacgccgc aacagcgagg tgtttgtgtc ggcggattgg cggttttgaa tggtgggata
     1801 atgccgtccg aactttggaa acaggttcgg acggcatttt tgcgcgttca ggcaagggcg
     1861 gcggcaaata cgccgcgcaa ggcgttggag agacggattt cttcggcttc ttgcagtgtt
     1921 ttttgtgtga tgtgtgtttc gatcacttga tttgtgtgca aatatttttg cggttcgtcc
     1981 aatacggctt ggcgcattat gccgtttaaa atgtctaaat ctaaagaggg tgtgagccat
     2041 tgtccgcgat gtttgacgaa gacgttgctt ctgccgcctt cgagcaggat gccgtctgaa
     2101 ttgaaaaaca ggctgtcgaa cgcgccttgt gtttcggcgg tttgccacgc ttggtcgaag
     2161 agggcgcggt gagtggtttt gaagtggcgc aggtagtttt gtgcgggcag gatggtcggc
     2221 gaaatgatga cgcgctgttt gtcagccaaa tggtttaaaa cggcgcggga caggctgatg
     2281 ccgtctgaag cgagcagggc tttgacgcgg aacacgccgt cgggcaagcg ggcgatgtat
     2341 tgtttgattt gattttcgca accgtcgggc aggggcaggt tgagggcttg ggcggcggct
     2401 ttcagacggc ataggtggcg gtcgagcagg gcgcattgcc tgttttccac gcgcagggtt
     2461 tcaaaaatgc cgaaatcggg gcgcaattcg ttgaggaaac gggctttcca gccgcattcg
     2521 cgatattcgg cggcggggtc gctgtcgatg acgatgccgg aaccgacacc gtacacgcct
     2581 tgataaatgc cgtctgaaag cggcgtgagc gataaggtgc ggataacgac gttgaacgcg
     2641 ccttcaaacc ccaagccgcc ggaacacggg ttcaaatagc cgatgctgcc cgtataaagt
     2701 ccgcgtgctt cggtttcgag tgtttcgata atctgcatac tcattttttt gggcgcgccg
     2761 gtgatactgc cgcagggaaa ggcggcgcgg aggatgtcgg cgaacgaggt gtccggcagg
     2821 gcttgcgccc ggatggtgct ggtcatctgc cagacgctgc cgaaacgcga tactttaaac
     2881 ggttcgggta cgcatactgt gccggtttgg gcgattttgc cgagatcgtt acgcagcaaa
     2941 tcgacaatca tcacgttttc ggcgcggttt ttcgggtctg cttgcaactc ggcggcgcgg
     3001 cgttcgtctt gtccgtcgcc caaaatcggc gcggtgcctt tcatcggttc ggtggcgacg
     3061 atgccgtctg aagcgatgtt gaggaagagt tcgggcgaga aacacagtgt ccacgcggat
     3121 tgcccctccg catcgggcag gtgggacaaa acggcatagg ggacgggctg gcgcaggcgg
     3181 cggtagaggc tgacgggatt gccgtaggct tgcaggtgca ggcgggtagt gtagttgatt
     3241 tgataggtgt cgccgcgccg gatggcttcg tggatttggc ggatatggtc gaggtaatcg
     3301 gcttcggata cggaggattg cggcgtggaa atgccggcgg ggaggccgtc tgaatgtcgg
     3361 gcaagccagc ttgcggcatc ggtgtcggcg cagtcggcaa accagtgcag ggcaagattg
     3421 ccgccgcgtt cggactcaac ccccgtcagc ggcagaccga atccgtagtc tgcaaacaac
     3481 accgaatgca gccctttttg ccagcccgat tgcagcgcgc cgtccaaagc atcgagttct
     3541 tcgggacgga aaaaacggct ttctacatga ttttgatagc gttttgcgcg accgcttacg
     3601 gcatcgtcaa acagggcgaa atagggcatg gcaattcggg gcaaatgttt tgattatacg
     3661 ctctttttac acatattttc agacgattcg gcaaatatcg gcaaaatgta attttatgta
     3721 gagaaagtgg ggggcgaggg tgtaaaattg tgaaaacaaa ttccgatttc caacctgaaa
     3781 tacaataagg agacctttat gacagaccac cagctgcaac cgtttgaaaa cgtagaatta
     3841 ggcgaaaagc aagaccagct ccaagtatag tggattaaca aaaaccagta cagcgttgcc
     3901 tcgctttagc tcaaagagaa cgattctctc aggtgctgaa gcaccaagtg aatcggttcc
     3961 gtactatttg tactgtctgc ggct
//
LOCUS       DAWFBO010000161         3930 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFBO010000161 DAWFBO010000000
VERSION     DAWFBO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.161"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..331)
                     /locus_tag="WGO87_001787"
     CDS             complement(<1..331)
                     /locus_tag="WGO87_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0393111.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVKEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQ"
     gene            complement(312..593)
                     /locus_tag="WGO87_001788"
     CDS             complement(312..593)
                     /locus_tag="WGO87_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ0393112.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(602..1396)
                     /locus_tag="WGO87_001789"
     CDS             complement(602..1396)
                     /locus_tag="WGO87_001789"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0393113.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1780..3114
                     /gene="gdhA"
                     /locus_tag="WGO87_001790"
     CDS             1780..3114
                     /gene="gdhA"
                     /locus_tag="WGO87_001790"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0393114.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3256..3630
                     /locus_tag="WGO87_001791"
     CDS             3256..3630
                     /locus_tag="WGO87_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393115.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3674..>3930
                     /locus_tag="WGO87_001792"
     CDS             3674..>3930
                     /locus_tag="WGO87_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0393116.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1069 a         1015 c          926 g          920 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttccttcacg ctgttttgag
      121 gaggcggcgt ttccgcatcg ggacgggcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgccgtccg tcagcgcgtg aatatcgcgg cacaggctca
      241 acagttccaa aaaatccgcc gacggcgaag tcagataacc gtccctgttc aggcggctga
      301 tcaggctgtc ttcacggtaa aggctgaaca attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcctgtctg tcgtccacac cgaacaggcg cagctccgca
      421 ccggaaccca gcgcgacacc tttccagaag aacacatttt cattgcgttt ttcatcttta
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaactgt caaaaaacgc ctgcgcccga aatgcctgcc cataccgcct
      601 cctaaaccga cactgccgcc ttgatgtgcg gatgggggtc gtaaccttcc aactcgaaat
      661 cttcaaactt gaaggaaaac aaatctttga cttcaggatt gattttcatc actggcaagg
      721 cgcgcggttc gcgttccaac tgcaatgcgg cctgctcgaa atggttgcgg tacaaatgcg
      781 cgtcgccaaa cgtatggaca aactcgcccg cctccaatcc gcacacttgc gccatcatca
      841 tggtcaacaa tgcgtagctg gcaatattaa acggcacacc aaggaaaata tctgcactac
      901 gctggtaaag ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc
      961 agggcggcaa ggccatttcg tcgaccaaag ccggattcca cgccgataca atcaggcggc
     1021 gcgagtcggg gtttttctta atttgttcca ccacattggc gatttggtcg atatgcctgc
     1081 cgtcgggcgc gggccagtta cgccactggt agccgtaaac cgggcccaag tcgccgtttt
     1141 cgtccgccca ctcgtcccaa atggaaacat tgttgtcctt taggtatttg atattggtat
     1201 cgcctttgag aaaccaaagc agctcgtgga taatcgaacg cagatgcagc tttttggtcg
     1261 tcagcagcgg aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg
     1321 tgcccgtacc ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt
     1381 ccaaataggc tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc
     1441 ggataatgcc caaaacacgg atacagacag gcaggattgt tggcaatttc agtccttttc
     1501 cacagtaaaa cccggtggga aaacaaaatt accttgattg gaatcaaaaa atctagttta
     1561 attacttaga ataaaatttc aataatattt ttatttacga aattaattta tgattattga
     1621 ttaattatcg gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt
     1681 ttgccaaatc gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt
     1741 tttcccatta gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctg
     1801 tttgccaacc tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa
     1861 gtcttcatga gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg
     1921 ctggaacgca tcgtcgaacc cgaacgcgtc gtcatgttcc gcgtaacctg gcaggacgat
     1981 aaagggcaag tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct
     2041 tacaaaggcg gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct
     2101 tttgaacaag tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt
     2161 tccgacttcg accccaaagg caaatccgat gccgaagtaa tgcgcttctg ccaagccttt
     2221 atgaccgaac tctaccgcca catcggcgcg gacaccgacg ttccggccgg cgacatcggc
     2281 gtaggcggac gcgaaatcgg ctatctgttc ggacaataca aaaaaatccg caacgagttt
     2341 tcttccgtcc tgaccggcaa aggtttggaa tggggcggca gcctcatccg tcccgaagcg
     2401 accggctacg gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt
     2461 gaaggcaaac gcgtcctgat ttccggttcc ggcaacgtgg cgcaatacgc cgccgaaaaa
     2521 gccatccaac tgggcgcgaa agtgctgacc gtttccgact ccaacggctt cgtcctcttc
     2581 cccgacagcg gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc
     2641 cgcgaacgcg ttgccaccta cgccaaagag caaggtctgc aatactttga aaaccaaaaa
     2701 ccgtggggcg tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa
     2761 gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg
     2821 ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga
     2881 aaagcctcca atgccggcgg cgtagcaact tcaggtttgg aaatgagcca aaacgccatc
     2941 cgcctgtctt ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc
     3001 cacgaatcct gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg
     3061 aacattgccg gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac
     3121 gccgccgctc cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttca
     3181 ttatccgccc gttcgaatcg ggtgatacta tttgtatcca gaattgttgg atagtttggg
     3241 agtggagagg aagaaatgat tatggaccaa tactatatgg agttaaagaa taagttatct
     3301 aacagaccaa tcctactgga taatactaat gattttcttt ttgttttagt aaatacagtg
     3361 aaagctatga tagaaaatac tgataaaagt cagctttcag agttagataa aattttagac
     3421 ggtgtcacaa gtcaagaatt gaaacttgcc tatgattttt gtcaagggaa attcggtcaa
     3481 gcaggttttt cttatcgtag gcacccgaat tatttttatt tgtccagttt aattgcgact
     3541 tttccagaat ttgagttgtc taaagcagac cgagattacc tcaaaggaat cataaatttt
     3601 gataactacc ttttatatga gttagattag tctttaatca cctcaaaacc ctatttattc
     3661 aaaaggagaa aaaatgattg agctgaaatt agtagatgag agcagttttc aggcagtgct
     3721 ggatttgaaa atatctgaag ctgatgaacg agcacgtttt gtggctccaa atgtacgctc
     3781 tttagctgac gcatggctct accgggaaaa tgaagatgtg tttccgaggg caatctattg
     3841 ggataagcaa gtggttggct ttctcctgct ggaaatagac aaggatgaag cagaatactt
     3901 tatctggcgg ataatgattg gtcagcagtg
//
LOCUS       DAWFBO010000162         3893 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFBO010000162 DAWFBO010000000
VERSION     DAWFBO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.162"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            150..653
                     /locus_tag="WGO87_001793"
     CDS             150..653
                     /locus_tag="WGO87_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0393117.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            762..1094
                     /locus_tag="WGO87_001794"
     CDS             762..1094
                     /locus_tag="WGO87_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393118.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            1091..1501
                     /locus_tag="WGO87_001795"
     CDS             1091..1501
                     /locus_tag="WGO87_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0393119.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            1506..2255
                     /locus_tag="WGO87_001796"
     CDS             1506..2255
                     /locus_tag="WGO87_001796"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0393120.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDIYRR
                     HKGRYGYRRIAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(2574..2936)
                     /gene="ssrA"
                     /locus_tag="WGO87_001797"
     tmRNA           complement(2574..2936)
                     /gene="ssrA"
                     /locus_tag="WGO87_001797"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2999..3604)
                     /locus_tag="WGO87_001798"
     CDS             complement(2999..3604)
                     /locus_tag="WGO87_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0393121.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT          986 a          906 c         1020 g          981 t
ORIGIN      
        1 attaaaagcc gaaaagaaga gtttaaaaaa ttaatggaaa agaagaaacc caaaatctag
       61 aacgagtttt aacccccttt aaaatcccat tcagacgacc ttttctaaaa tccctgtatt
      121 gatttttaat tcaatacagg gatttttcca tgtcagacga attcaaccga tttatcgaac
      181 gcgtcctctc ccacgagggc ggttacgtca atcatcccaa agaccccggc ggcgaaacca
      241 attggggcat cactaagcgc accgcacagg caaacggcta caacggctcc atgcgcgcca
      301 tgacgcgtga acaggcaatc ggcatttacc gtaaagcgtt ttgggagcgt taccgcgccg
      361 accaaatgcc cgaagcggtc gcttttcaat tttttgatgc ctgcgtcaac cacggttacg
      421 gcaatgccgc ccgtatgctc caacgcgccg caggcgtgcc ggacgatgga atcatcggcg
      481 aaatcagcct caaagccatc aattcccttc ccgaaaacga ccttttattg cggttcaacg
      541 ccgagcgtct ggtcttttat accaagctgg gtacgttcac ctctttcggc aagggctggg
      601 tacgccgtgt ggcgcaaaac ctgattcacg ccgccgcaga caatgccgat tgaggagcaa
      661 accatgtcaa aaaagtccat tctcgccctg atggccgcag ccatgcagcc cgatttcagc
      721 cacagcgacc taggcattcg ctacgccatg ccgactcagg gatgttggac gcaagcccac
      781 cgcaagagcg gggtagccgc cgcgaaacgc gcagccaaaa aaaagcgtca caaataaccg
      841 cctttttccg atggctgggc ggcttggtct ctaatccggc cacagggaaa atcagccata
      901 ccaaactttg ggcaaacgtc gccgcagccg ccatgacttg gaagttcgtg caggcggcgg
      961 acgcgcccga atggctttgg tgggcatacg gcgcgatggt cggcgggtat gcattaatca
     1021 aacgcggcat cgccgtcgta ccgcagttgg cggaagtcaa aaaatccgca aatcaggaag
     1081 gggggcggca atgattgaat ttgtccgagc caaaaaacgg ctgctttggg catttgtgct
     1141 tttgcttgtg tggacgtgcg gttaccgata cgccgccgac aaggccgaag cggaacaaac
     1201 cgccctgatt gccacctatc ggcattcttc tatggttgcg gcggaacaat atgccttgca
     1261 gcttaaaaaa gcgcaggacg aaaggcagcg gtggtacgac ttttcccaaa aacaaggaag
     1321 aaagcccgtg aaaaaacagt atccgccgca aacgaaaaaa gccggctatc tgaaaaccaa
     1381 ggaagaactg cttgcggaat tggcttgcct taaagcggaa atggttgccc taaaaaagcc
     1441 cgatgcctta atccatggga aagaagtgcg gcagaaagaa cgcaactcgt cgcagggtta
     1501 aggcaatgcc atccgttgaa ctgctgttgg agattgtcgg actgccacgc ggcaccttct
     1561 attaccaatt ggccgtccaa tcggcagaag acaaatatgc cgatttgaaa cggcatatcc
     1621 atgatattta tcgacgacat aagggaagat acggctaccg gaggattgcg gcagccatcc
     1681 gtcacgcagg aacaccggtc aatcacaaga aagtcagccg tctgatggcg aagacggggc
     1741 tgaaggcagt gatacggcgg cgcaaatacc gctcgttcaa aggagaagtc ggcaaaattg
     1801 cgccgaatat cctgcgacgc tgtttccatg cagaaaagcc gaatgagaaa tgggtaacgg
     1861 acgttaccga gttcaatgta ggcggagaaa agatatacct ttctccgatt atggatttgt
     1921 ttaacgggga aatcgtcagt taccgtattc aaatccgccc gactttcgat ttggccggcg
     1981 agatactgaa aggtgcgccg gagaaaccgg gatcgtctga aaagccgata ctgcattcgg
     2041 atcaaggttg gcaatatcag atgttttttt atagtggatt aaatttaaat caggacaagg
     2101 cgatgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgccg tactggttta
     2161 aatttaatcc actatacaac aacgatagaa tcaagttgaa attaaaaggg ctgagccttg
     2221 ttcagtacag aattcagtcc ctgaaagccg cttgattaaa ctgtccgact ttttggggtc
     2281 agttcggctt cggcattttt ttatccgttt gggggtaact tgtttggaaa gctgcaagct
     2341 tataaataaa ggattacatt taagttttgg gtaacctttt taaaaaaatg cgtgatgact
     2401 tttgcatttt taaggcgttt tttggggtaa ttcgtgaaaa gttaccccaa aagttacccc
     2461 ataaatggcg aaaactcaag catacgccag catattcaag gcaacaaaaa agccttgaaa
     2521 ctgttgaagt tcaaggcttt tttgtgttgc aggatgctgc tgaaaatagg gtatggtgga
     2581 ggcgggggga atcgaacccc cgtccgaaag tcctctacaa agcgttctac atacttagtt
     2641 gtgtctattt gaaaatctta tttccatcat gccgaccaac aggccttttg gaaaccagtt
     2701 accttaaatc tcatttcctg ccaagtaacc cggcaggaaa ccagtcaatg taagatgacg
     2761 ttgcggtggc tttcgccaca cagcccattg accgactgct gcaacggcta gccttaagcg
     2821 gctaaagcgt aagtttcgtc gtttgcgact atttgaattc agtgttttac gggaatctga
     2881 gaccccggta tgcccgcatc tgcttcgcaa cccccgtcga aaccaaggtc gcccccagaa
     2941 atggtttgca aattatacgg atattgtgcg gtgctgccaa gtctgtcgga gaaatttgtc
     3001 agtcttgctg ccttaatttg cgtttgagca ggatgcggac gcagccgtcg ttgccttccc
     3061 ggggttcggc gtaggcgagt acatcggggt gttgcatcag ccagtttcgg gtcatatttt
     3121 tcagaacggg tttgtagcct ttggaaccta atccgctgcc gtggatgatt tcgccgcata
     3181 cgccgcgttt ttgggtgaat gcgatgaatt cgttgaggac tttttgggct tcttcctgtg
     3241 tgtagccgtg caggtcgaca tcggtaacga cgggatagta tccgtttttc aggcgttgga
     3301 tgtcgttttt tccctgtccg tttttgctga agctggcggg cgggtcgttg tatgtgctgc
     3361 cgatgtagaa gtagttttct tcgtcggcgc ggttgtcttt gggacggact ttgatggggg
     3421 ttttgtcggg cggcgcataa tattgctgcc ggttttttaa tggggagact tgtccgactg
     3481 cttgtgaaaa atcgaaatcc tgttcttgtt tttgcttgtt tttttcggct gcctgttttt
     3541 ctgccgcttc tttttgggct tgtttgccca gttgtttgag gatgttttgg aagtcggtat
     3601 tcatattttt tcctgttatt tgtccgatgg ctgtttcggg cggggtttta atttgccgga
     3661 atgtttgcca atcgggggag gatgattttg ttgcctgcgt atgttttttg aaagtgtgat
     3721 tgtatatcaa aaagaaatgc ggcaaccgtc ggcagtgttg attgccggaa atgcggaccg
     3781 gtcgaaccga tatgcccgaa cgcctgataa agttttaaaa acctgccttg cgaagcaggc
     3841 tgacgtgttt tgccaatctt gaattgccgg aaacgcgaaa cacggaaatc tga
//
LOCUS       DAWFBO010000163         3886 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFBO010000163 DAWFBO010000000
VERSION     DAWFBO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.163"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..2118
                     /locus_tag="WGO87_001799"
     CDS             <1..2118
                     /locus_tag="WGO87_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0393122.1"
                     /translation="GKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFGG
                     ESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTAGVQVGSAQLIKGASTAVNGMDPE
                     GAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKEFGVRANGKLRH
                     GDTPRHGYSEDNKEFALNADYRGETLRVAFDSIYAKRKTNGGRARIQDIQNAGGRLFG
                     APDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVCL
                     NATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLTARGEFETGPVTHN
                     WSMAFDRVIRQRKTINGSGNGNSKIEVKANENIANQLASFTADYPNSWENSANLDANI
                     KVNSLALSDTLGFVDNKYRLTLGERFQTVEYTDKKSQSGDAKRFSPMLMAAWVPQPDL
                     VVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKRPG
                     YWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANLLN
                     NTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNGN
                     VSYFGKSYQDTQKQYAFPSYTLIDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQRG
                     QYDRSFAVVGLPRTYWLKAELDF"
     gene            2242..3792
                     /locus_tag="WGO87_001800"
     CDS             2242..3792
                     /locus_tag="WGO87_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0393123.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         1081 a          968 c          987 g          850 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcaccgatt accgtcgtca attacgacga acaagccctc
       61 aataacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gcgaaagcaa cacgctgacc ggcctgtatt tccgcggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggcttggcc ggtatgtacg gcacgcaagg caccgccggc
      241 gtgcaagtcg gctccgcgca actgattaaa ggcgcgtcca ccgccgtaaa cggtatggac
      301 cccgaaggcg cggtctccgg ttccgtcaat atcgaaacca aaaaagccgc cgatgaaggc
      361 aaccgcaaaa tcggtttggg ccggtttagc aacaaccgcg cccaaggtac gttcgacttg
      421 ggtcaacgct tcggcgaaaa caaagaattc ggcgtgcgcg ccaacggcaa actgcgccac
      481 ggcgacactc cgcgccacgg ttacagcgag gacaacaaag aatttgcctt gaatgccgac
      541 tatcgcggcg aaacactgcg cgtggcgttc gactccatct acgcgaaacg caaaaccaac
      601 ggcggccgtg cgcgtattca ggatattcaa aacgccggcg gacgcttgtt tggcgcgccc
      661 gatggtaaaa ccaacctgct gccttcttgg aactggcaaa ataccgccgg ccaaaccaat
      721 atgcttacgt ttgaatggga tgcgtttgat aatgcacaga ttacaggcgg tatcggctac
      781 aacaaggcac gctattacgg cacgctgatt tccccgaccg tatgtttgaa tgctaccagc
      841 acttgtaccg atatgcaaaa cacaaacgcc agcggcaaag taacggcacg caactatgac
      901 tacaataccg gtactgcgcg cctgaccgac caatatttcc gcacattgag tatgaacctg
      961 accgcacgcg gcgaatttga aaccggcccg gtaacacata actggagtat ggctttcgac
     1021 cgcgttatcc gccagcggaa aaccataaac ggcagcggga acggcaacag caagatagaa
     1081 gtgaaggcaa acgaaaatat tgcgaatcaa ttggcatcgt ttacagccga ctatcccaat
     1141 tcttgggaga actctgccaa cttggatgcc aatatcaaag tcaacagcct ggctttgtcc
     1201 gacaccttgg gcttcgtgga taacaaatac cgcctgactt tgggcgaacg tttccaaaca
     1261 gtcgaatata ccgacaaaaa atcacagagc ggcgatgcca aacgtttcag cccgatgctg
     1321 atggcggcgt gggtgccgca acctgatttg gttgtgtacg gcaactatat ggaagacttg
     1381 gagcctgccg acatcaaaac cgatgattcc ggcgaaacga caatggcgaa accgcgtgtc
     1441 agccgccagt ttgaagtggg cgtgcgtaaa aactggggca atttcgtaac cacgttaaac
     1501 gcgttccaaa tcaaacgccc gggctactgg cgcggtcata caactaccaa gacaacaaaa
     1561 ggggtaacaa caacgctgac ctatggcaac aattccgatt tcgcccgcta taaggcgcaa
     1621 ggcggcgcgg ccggcgacga gcaaggtatg gaacgcaatc gcggtattga gttcaacacc
     1681 tacgcgaact tgctcaacaa tactttgcgt ccgactttgg gtctgatgta tctgcaatcg
     1741 accgtgaaag agtatccaaa ttcacgcgat atgctggtta acggcgtaca agtcgccaat
     1801 ccgcgcgtaa ttgccaaagc aggtttggaa tgggatacac cgtttgccaa aggattgact
     1861 ttaaacggta atgtttcgta tttcggcaag tcttaccaag acacgcaaaa acaatacgcc
     1921 ttcccgtcct acaccttgat tgacgtaggc gcgcgctaca aaaccaagct cggcaaaaat
     1981 actttgaccg tcagcagctc ggtggaaaat ctgttcaaca aaaactactg gcaggtacag
     2041 cgcggccaat acgaccgcag cttcgccgtt gtcggtctgc cgcgtactta ctggctgaag
     2101 gcggaattgg atttctgatt tctccgcatc tggaaaatgc cgtccgaaca ggtttcagac
     2161 ggcatttctt ttaaagagat atggcgggat acgcttaaat aatgctattc tagcgtcttt
     2221 gaaacctgct tgtttgaagt gatgaaaaaa cctttgattt cggttgcggc agcattgctc
     2281 ggcgttgctt tgggcacgcc ttattatttg ggtgtcaaag ccgaagaaag cttgacgcag
     2341 cagcaaaaaa tattgcagga agcgggcttc ttgaccgtcg aatcgcacca atatgagcgc
     2401 ggctggttta cctctacgga aacgacggtc atccgcttga aaccggagtt gctgaataat
     2461 gcccggaaat acctgccgga caacctgaaa acagtgttgg aacagccggt tacgctggta
     2521 aaccatatca cgcacggccc tttcgccggc ggattcggca cgcaggcgta cattgaaacc
     2581 gagttcaaat acgcgcctga aacggaaaaa gttctggaac gcttttttgg aaaacaagtc
     2641 ccggtttccc ttgccaatac cgtttatttt aacggcagcg gtaaaatgga agtcagtgtt
     2701 cccgccttcg attatgaaga gctgtcgggc atcaggctgc actgggaagg attgacggga
     2761 aaaacggttt atcaaaaagg tttcaaaagc taccgcaaca gctatgatgc gcccttgttc
     2821 aaaatcaagc tggcagacaa aggcgatgcc gcgtttgaaa aagcgcattt cgattcggaa
     2881 acttcagacg gtatcaatcc gcttgctttg ggcagcagca atctgacctt ggaaaaattc
     2941 tccctagaat ggaaagaggg tgtcgattac aacgtcaagt taaacgaact ggtcaatctt
     3001 gttaccgatt tgcagattgg cgcgtttatc aatcccaacg gcagcatcgc accttccaaa
     3061 atcgaagtcg gcaaactggc tttttcaacc aagaccgggg aatcaggcgc gtttatcgac
     3121 agtgaagggc agttccgttt tggcacactg gtttacggcg atgaaaaata cggcccgctg
     3181 gatattcata tcgctgccga acacctcgat gcttctgcct taaccgtatt gaaacgcaag
     3241 tttgcacaaa tttctgccaa aaaaatgact gaggaacaaa tccgcaatga tttgattgcc
     3301 gccgtcaaag gcgaggcttc tggactgttc accaacaatc ccgtattgga cattaaaact
     3361 tttcgtttca cgctgccgtc aggaaaaatc gatgtgggcg gaaaaatcat gtttaaagat
     3421 ttgaagaagg aagatttgaa ccaattgggt ttgatgctga agaaaaccga ggcagacatc
     3481 agaatgagta ttcctcaaaa aatgttggaa gacttggcgg tcagtcaggc aggcaatatt
     3541 ttcagcgtca atgccgaaga tgaggcggaa ggcagggcaa gtcttgacga catcaacgag
     3601 accttgcgcc tgatggtgga cagtacggtt cagagtatgg caagggaaaa atatctgact
     3661 ttgaacggcg accagattga tactgccatt tctctgaaaa acaatcagtt gaaattgaac
     3721 ggtaaaacgt tgcaaaacga accggagccg gattttgatg aaggcggtat ggtttcagag
     3781 ccgcagcagt aatgggtcaa atgccgtctg aaatgttcag gcggcatttt gttgtattgg
     3841 aaactgccgt tttcaaccat tcctttagat aagccgtgtc ggattc
//
LOCUS       DAWFBO010000164         3859 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFBO010000164 DAWFBO010000000
VERSION     DAWFBO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.164"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WGO87_001801"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WGO87_001801"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0393124.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WGO87_001802"
     CDS             1439..1924
                     /locus_tag="WGO87_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0393125.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGO87_001803"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGO87_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0393126.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          783 a          941 c         1078 g         1057 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcgaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccaac ggtcgtgcga
     2161 aatcaccatc acgctaccgg caaattccag caatgcgtct tccagcgcgc gcagggtttc
     2221 tacgtcaagg tcattggacg gttcgtccaa cagcaataca ttgccgccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWFBO010000165         3797 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFBO010000165 DAWFBO010000000
VERSION     DAWFBO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.165"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(556..993)
                     /gene="rnhA"
                     /locus_tag="WGO87_001804"
     CDS             complement(556..993)
                     /gene="rnhA"
                     /locus_tag="WGO87_001804"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0393127.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1106..1975
                     /gene="tehB"
                     /locus_tag="WGO87_001805"
     CDS             1106..1975
                     /gene="tehB"
                     /locus_tag="WGO87_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ0393128.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEILLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSMYCTAADYFH
                     KKYGMSATHSAVSAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2025..2831)
                     /gene="thiD"
                     /locus_tag="WGO87_001806"
     CDS             complement(2025..2831)
                     /gene="thiD"
                     /locus_tag="WGO87_001806"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0393129.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
BASE COUNT          816 a          926 c         1098 g          957 t
ORIGIN      
        1 gggtgtaacc ccttccaaat caggacgaca catagggagg tgctttatgt gtcgtcctgt
       61 gtgttgaaat atatcggacg cgtttgaaca gttgtgcggc aaactgtttt agcggcaggc
      121 aagagatgcc gtctgatgcc gtctgaagcc cattgtccgt cattcccaca caggcagttg
      181 ctacggttgc tgaagcccct tagccgtcgc gcaggcggtt gctacggtta ctaaaatccc
      241 gtcattcccg cgcaggcggg aatccagtcc gttcggtttc agttgtttct gataaatctt
      301 gcagcgttgg gtttttagat tcccgctttc gcgggaatga cggggcggtc ggatgccgcc
      361 tgaaactccg tcattcttgc aacttttcgt cattcccgtg aaaacgggaa tctagaactt
      421 caaactttca gataatcttt gaatattgcc gttgcccgac ggtttggatt cccgcctgcg
      481 cgggaatgac ggtggaggac gatgccgtct aaagcccatt agcggtttca gacggcattt
      541 ttgccggagc ggcagtcagg aaaactgcgc tgcgccacgg tttgccaaat cgtcggcgcg
      601 ttcgttttcc gcgtgtcccg cgtgtccttt aacccaagtc cagctgactt ggtgctgccc
      661 aaccagaacg tcgagttctt tccacaagtc gtcgtttttg acgggctgtt tggcggcggt
      721 tttccagccg ttgcgcttcc aaccgtgtat ccagttttcc atgccgtttt tgacgtattg
      781 cgagtcggtg cagatgatga cggtgcagcg gcgtttgagc gatttcagtc cttcgatgac
      841 ggcggtcagc tccatgcggt tgttggtggt ttgcgcctcg ccgccgaaaa gttctttttc
      901 gtggctgccg tagcgcatta atacgcccca gccgcccgcg ccgggattgc ctttgcacgc
      961 gccgtcggtg taaaggtaaa cgggtgtgtc catattgtgc ctttgtgtgg tgtccggcgt
     1021 gattataagc ggtttgcgcg ccgtgccgtc tgaaagattg cggcggattg aaataggcat
     1081 ataatgtgca actttatttt tgaatatgaa agagcggata gtgggtcaaa gcggcgagtt
     1141 gttttgtttc ggacaaatgc ctgtatggaa agtggaaaac ctgccggaaa ttttgttgtc
     1201 gggctattcg tctgaggaag gggaatgggt ctgcctgaat gtgttgcagg gcgatgtcga
     1261 agtccgtgca ccggacgggt cggcagaggt ttggtcggca gacggcggcg attgtgtgtt
     1321 tgcgccgcag caggtgtttt cggtcaaacc gaaaacggac gatgccgaaa tccgcttgtc
     1381 gatgtattgc acggcggcag actattttca caaaaaatac gggatgagtg ccacgcattc
     1441 tgcagtgtcg gcggctcagg ataccgtacc tgcgggcagg gcgctcgata tgggctgcgg
     1501 acagggacgc aacgcgctgt ttctcggttt gaaggggttt gaagttactg ccgccgattg
     1561 caatcccgcc gccttggcaa acgtggcgga gctggcagag gcggagggtt tgaacgtccg
     1621 cacgctggaa tatgatttga acgccgccgc cttgcagggt gagtttgatt atattgtggc
     1681 aacagtggtg ctgatgttcc tgatgccgca gcgcgtgcct gacgtgattg ccgatatgca
     1741 ggcgcatacg gcggcgggcg ggtacaattt gattgtatcg gcaatggata cggcggattt
     1801 cccctgcccg atgccgttcc cttttaaatt taaagaggga gagctgaagg attattatcg
     1861 ggattgggag ctggtcgaat ataaagaaga attgggcgcg atgcacgcca aagacgaaaa
     1921 cggcaatccg atacggttta aatttgtaac gatgctggcg aaaaagcctg aataatcctc
     1981 aaaaatgccg tctgaacatt ttgtttcaga cggcattttt acggttagtc ccgataggcc
     2041 caatgattga ccgggccgtg tcccgcgccg atttccaaag ggcttgagat tgccgccgtg
     2101 atgtaggact tggcagtccg tacggctttg caaacgtcta agccttttgc caactcggcg
     2161 gtaatgcagg cagaaaacgt gcagcccgtg ccgtgcgtgt gggcggtcgg aaagcgcggg
     2221 ctgtccaatt ccagcgtttc gttttgcgta aacagccaat ccgtgcagcg tccgctgctg
     2281 ctgccattca aatgtccgcc tttgatgacg acatttttga caccgtaatc aagcaggatt
     2341 tttgccgcac gttccgcatc tttacggttt tcgatatgca cgccggtcag ggcttccgcc
     2401 tcgggcaggt tgggggtcaa tacatccgta tcgggaagca gcaggcgcgt cagtgccgca
     2461 acggcggaat cctgcaacag cggcgcaccg cctttggcaa tcatcacagg gtcgagtacg
     2521 cgcctgccga agtcgcagtg tttcagcttg tcggcaacgc attcgatgat ttccgccgtg
     2581 ccgagcattc ctattttgta ggcgcggatg tcaaagtctt ccctgattgc ttggatttgt
     2641 gcggtgatgg tttcggtcgg gacgagatga accgccgaca cgcccaaggt attttgcgcg
     2701 gtaacggcgg tgatgacgca cgttccgaac acgccgcgca tctgaaacgt tttcaaatcc
     2761 gcctgaatgc ccgcaccgcc gcccgaatcc gaaccggcga tggtaagcgt ttgcacaaaa
     2821 gagcttttca tttgttcctc cttaatattg tgtccgataa aaagggctaa tgcctgtaaa
     2881 aaagcacggc gatgccgtct gaaaaccgca gcgcggggtt tgtggtgcat tatatgccgt
     2941 attgcaggac aaggcacagg gcagggcggc ggggtgcatc gtagcaactg tatttttgcc
     3001 cgacggggtg aaaaatacag ttgctacacc cactgataat gaaaaaagag agcaatgccg
     3061 tctgaaccgc gtgttgcggc gagcataaac cggcacgctt tattcgtcat ttccacaaaa
     3121 acagaaacct aaaattccgt cattcctacg aaagcgggaa tccagtgtgt tcggtttcag
     3181 ttgtttttgg gtttcgggtg atttccaaat catcattccc gcgcaggcgg gaatctagcc
     3241 cttggggcag cggcaatatt tataaagccg tctgaaaatt cagaagttct agattcccgt
     3301 tttcacggga atgacgaagg gttgtgggaa tgacgaagtt tcagacggca tcgccccgcc
     3361 ccgtcattcc cgcataagcg ggaatccaaa ccattgggca gagcagcctt aaaggttata
     3421 aaagactcgt cattcccacg aaagtgggaa tccagacctg tcggcacgga aacttatcgg
     3481 ataaaacggt ttcttcaacc ctacgctcta gattcccgct tttgcgggaa tgacgaagtt
     3541 tcagacggca tcaaatgctt tgtcaggctt cagacagcat cgtccaccct ctgccgtcct
     3601 tcccgcgcag gcgggaatcc aatgtgttcg gtttcggttg tttttgggtt tcgggtaatt
     3661 tccaaatcgt cattcccacg aaagtgggaa tccagaccat tgggcggcgg aaatattcaa
     3721 aagtcgtctg aaaattcaga ggttctagat tcccgttttc acgggaatga cgaagggttg
     3781 tgggaatgac gaagttt
//
LOCUS       DAWFBO010000166         3751 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFBO010000166 DAWFBO010000000
VERSION     DAWFBO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.166"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(278..2710)
                     /gene="hupB"
                     /locus_tag="WGO87_001807"
     CDS             complement(278..2710)
                     /gene="hupB"
                     /locus_tag="WGO87_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ0393130.1"
                     /translation="MPIPFKPVLAVAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDIEIKNDGYVYNPTDTNGPSKWLDYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDKRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGKGDNANSDYSYFAKLYDPKILTSNDGKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPELKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSP
                     VWQNQNRSAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVNNHTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(2741..>3751)
                     /locus_tag="WGO87_001808"
     CDS             complement(2741..>3751)
                     /locus_tag="WGO87_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein"
                     /protein_id="HEZ0393131.1"
                     /translation="AEPHVPVSIPTATPLPKGEVTLSSDNGNIENINTANTETHPPRR
                     TRRSLYASPQNTSSGISIQQREVEKIYFGVKSPEKSFIFQTPGGAQYALSSYADPIVP
                     SYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIGPNGEID
                     LFAGGFPVGKTPPPAFSYGNSTPETALSKGKITYQVWGIRVRNRQFVTSSYTPPKSGS
                     YYGTLANTPVLSFITANFNSNKLAGEIRGNSDYGPSVKIENATINSLSFSGTATSGGK
                     TGNLEGKFFGKFNGYRDTETSIGGKITFDGDRSLDTVFGGVSYKKELENNTDTSTTHL
                     TK"
BASE COUNT          688 a          867 c         1182 g         1014 t
ORIGIN      
        1 gggaatgacg tggtgcaggt ttctgtgcgg atagcttcgt cattcccgcg caggcgggaa
       61 tccaggttcg ttgagtttca gtcatttccg ataaattctt gcagctttgc gtttctagat
      121 tcccacttcc gtgggaatga cggagagtcg cgggaatgac ggagagttgc gggattgggc
      181 ttggtacaag cctgaaaggg ggcggcggtg cgactgtaaa aaatgccgtc tgaaacccaa
      241 atcatcgggt ttcagacggc attggcacat caggagctta gaacttcgct tcgatggtga
      301 aattgtagct cctgcccggc gaggtgaaac gttggatgcc ggcgtgggtg tggttgttga
      361 cgcggttgac cgtgccgaac tcgcggatgc tgcgtaaaga ttcccaagtg tagtactgct
      421 tgttggtaat gttgtacgcg gcggcgcgga gcgtaacctg tttgccgatg ttgaggtagg
      481 cggaaaggtc gaacagcgta taggccttgc tgtgtttggc ataaggccat gggttgttca
      541 agtcgtcgtt gctgtggacg gtgtcggacg gctttttggc ggcggtgcgc gtggcgtagg
      601 cgttgatgcc ccagcgtttg gaaggcgcgt catagcccag gctgtaaacc gccgtccacg
      661 gcgaaagcgc gttgatgggc gtttccttgc cgttgttttg cgttgccttg cccttgatgt
      721 agctgacgtt gaggccggtg tgcagccctt tgggcaggcc gatgctgtcg aggttccaag
      781 tgccgttaaa ctctatgcct ttcacccagg cggcggagcg gttttggttt tgccaaacgg
      841 gcgagctgac caatgccgtg ccgtctgaaa gcggggcgta tctggggttg tttttatcgt
      901 ctgacgaaac gcccatgtag gtcagttcga taaagtcgcg gtatttggtt ttgaagcctg
      961 agagcttgaa gttgcccgcc ttgccgctgc ccgccagacc caattcccag tttttggctt
     1021 tttccgcttt cagttcgggg ttggctttca ggtagaaatc ggggtgtggg aacagcagcc
     1081 aggtttcgtc cgaagtcggc gcgcggaagc cggtgctgta ttttgccaac aagtgcagat
     1141 gcttggtaaa ccgccagtcg aaccccgtgc cgtagctgaa gcccgcgtgc gcgcgttccg
     1201 aacccaaatg gggaatctgt ccccggatgg cggtggtgta tttcggatcg tctttcgcgc
     1261 tgctgctgtt tttgtcgtag cgtatgcccg cattcaggcg gaagcggtcg ttgccgccca
     1321 agtgaaacgc attgttccaa taggcaaatt tatatttcga ccggttttcg atcagcatcg
     1381 tgattttgcc atcgttgcta gtgaggattt tggggtcgta cagttttgcg aaatagctgt
     1441 aatccgagtt ggcattgtcc cctttgccgc cgcccaagcc gtattgcgcc gcccaaacgg
     1501 ctttggaaaa gtcgagttgt ttttcaaaat cggcagtcca ttgcgcggtg ttttggcgga
     1561 tatgccggaa cgaatggtaa acctcgccgt tgatgcctct tttatcgtaa tttttcggga
     1621 tgtcccaagt ccaagtgttc atatcgatgc gctgcttgtc gtagcgcagc ttaaggctgt
     1681 cccacgggcc gtgttccaat tcgtttttgt attcgacgcc ggagcggcgg cggtagctca
     1741 cgtcttggcg gtggcggtag tcgcccgttg cggcagacgt ggtcgtaccc gtccacaaat
     1801 tagacagctc gttggtaaaa cggtcggtgc gcgagtcttc aaaaatccag ccgatacggt
     1861 tttgatcgtt gaagttgtag cccagcttga acaaggtgct tttgtttacc cactcttgcg
     1921 ggtcgggttg ggagcgtgca acccctgtgg ctacgtaatc cagccatttg ctgggtccgt
     1981 ttgtatcggt cgggttatag acatatccgt cgtttttaat ttcgatatcg ccctcggtcg
     2041 agcggttttt ggtttctttg ccgaagcggc gggtataaac caataaggca tccaaaccaa
     2101 agagcctgcc ggcggcggtg atgctgctga atttttggct gtttttgccg acgctgccgc
     2161 cctttatccc caaatggtag ggcttgtctt cggaaacata atcgcttgcg gatttggttt
     2221 ggtaattgac tgcgccgccc aatgcgccgc tgccggattt gagcgagtcc gcgcctttgg
     2281 tgatggttac ttcggaaaag ttttccggct cggaagtgtt gcggttggcg ttgaagttgc
     2341 cgtacgcgcc gaacaattct tggaaggctt cggaagagcg gctttccgcc tgcgccagcc
     2401 cgtcaacgtt gatggcgacg cggtctttgt ccacgccgcg tatggtaaag ccgttagaac
     2461 ccgccctgcc gccttcgacg acggaaatgc cggggtcgta gcgcaccagg tcgtgttcgt
     2521 cgttgaccaa gagcttgtct aaggttttgc ggcggatttt ttcttcgccg agtttttggg
     2581 ttttgtgcgt gccggtaacg gtgatttcgt tcagcgtttg ggcggactgc ggcgcggggt
     2641 ctgccgcaaa ggcgggaaac gcttgggcga tggcggcgac agccaatacg ggtttgaagg
     2701 ggatgggcat aagaaaacct ttttaaaagc ggattgatta ttattttgtg agatgggtgg
     2761 tacttgtatc agtgttgttt tcaagttctt ttttataact cacaccgccg aatacggtat
     2821 cgagggagcg gtcgccgtca aaggtgattt tgccgcctat gctggtttca gtgtcccggt
     2881 agccgttgaa ttttccaaaa aacttgcctt ccaaattgcc ggttttcccg ccggacgtgg
     2941 cagtgccgga aaaggataaa ctgttaatcg ttgcgttttc aattttcaca ctcggtccgt
     3001 aatcgctgtt gccgcggatt tcgcctgcca gtttgttgct gttgaaattg gcagtgataa
     3061 aggaaaggac gggggtgttg gcaagtgtcc cataataaga accgcttttc ggcggcgtat
     3121 aagaggaagt aacaaattgc ctgtttctga cgcggatgcc ccaaacctga taagtgattt
     3181 tgcccttcga caatgcggtt tcgggcgtgc tgttgccgta ggagaacgca ggaggtggcg
     3241 ttttgccgac ggggaagccg ccggcgaaga ggtcgatttc gccgttggga cctatccacg
     3301 ccccgaattt catataatct tgtttggtaa tttccgcata cgaagtagcc ccggagtcgc
     3361 tgcagcagat aaagatgcgg ctgccgtcgg caagccgctg ccctgcgtgg cggtcgggaa
     3421 ttttgaaatc gggggaggag tagctgggga cgatggggtc ggcgtaggat gaaagggcgt
     3481 attgcgcgcc gccgggtgtt tggaagatga atgatttttc tggtgattta acaccgaaat
     3541 agattttttc tacttcccgc tgctgaatgg aaataccgga ggatgtgttt tgcggggagg
     3601 catagaggga gcgtctggtg cggcgcggcg gatgtgtttc agtgttggcg gtgttgatgt
     3661 tttcgatatt gccgttatcg cttgataacg ttacctcgcc tttgggcagc ggcgtggcgg
     3721 tggggatgga cacggggacg tgcggttcgg c
//
LOCUS       DAWFBO010000167         3751 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFBO010000167 DAWFBO010000000
VERSION     DAWFBO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.167"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(214..1278)
                     /locus_tag="WGO87_001809"
     CDS             complement(214..1278)
                     /locus_tag="WGO87_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0393132.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRENRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIIH
                     NGIDTDRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTENIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="WGO87_001810"
     CDS             complement(1279..2037)
                     /locus_tag="WGO87_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0393133.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="WGO87_001811"
     tRNA            complement(2112..2187)
                     /locus_tag="WGO87_001811"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3377)
                     /locus_tag="WGO87_001812"
                     /pseudo
     CDS             complement(2241..3377)
                     /locus_tag="WGO87_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            complement(3322..>3751)
                     /locus_tag="WGO87_001813"
     CDS             complement(3322..>3751)
                     /locus_tag="WGO87_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0393134.1"
                     /translation="LAADEVFEDPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNG
                     RVYTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSSFIHYRINHSKEFAD
                     RQNHINGIENFWNQAKRVLRKYSGLARTFPRIGSATGCAD"
BASE COUNT          854 a          922 c          981 g          994 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc agtttatatt gcatccaata atttgtcggc
      241 ggtatggttg atggtaaaac gggcggcgac gcattgatga gctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcgtcc gccaaagatt caggcgtaag
      361 cctatccagc agaatccccg attgatgatg ttcgacaatt tccttttgcg cccccaaagt
      421 attggaaatc accgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgttttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatcaggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcaat atcacacagg cttcaattag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaccg
      781 atcggtatcg ataccgttat gaataatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtggtaa aaatcggttt tgttggcgac gacattatgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggttttccc ggataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat tgtgcagccc cgaagcccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccacc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccagatttg tacagataat atagtttgac
     1321 atatttaatc atcgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 aagaaaccct ttgttcagaa tataaatttt gaaaaaaccc caaatcggac ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg agatggaagt
     1501 atatttgttg aacttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttgg ggtagggggt ttgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgtct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ctcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc acccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcgtcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt cgtttttggc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggca
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gcaggttggg gtcgttctgc ccgtcccaaa gccggggcgg aagttttcct
     2401 tcgggattgc tttgccggtt ggcaatgccc cacgcgaacg cggcttcgat tgcgtccgcc
     2461 gcgcccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcgcggct ttccatattg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttacgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattttc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgggaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagcccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacatgcc ggtcagcaac
     3121 aattcgccta catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atgcgtccgt cagtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatc ctcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccactat attttcgcaa gacgcgtttt gcctgattcc aaaagttctc
     3421 aatgccgtta atgtggtttt gacggtctgc aaattccttg gaatggttga tgcggtaatg
     3481 gataaaactg ctcacgtcca acttgtcgca gctgctcaga ctatcggtat aaacaatact
     3541 gtccggcatg attttctttt tgatgacagg gagtaacgtt tcagacttgg cattgtttac
     3601 gacaacggta tagacccgtc cgttgcgttt cagaatgccg aaaacaacca cttttcctgc
     3661 cgcaccgcga ccacgtctgc ctttacgccg tccgccgaaa tcgctttcgt ccggctcgac
     3721 aggatcctca aaaacctcat cggcagccaa g
//
LOCUS       DAWFBO010000168         3730 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFBO010000168 DAWFBO010000000
VERSION     DAWFBO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.168"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(228..551)
                     /locus_tag="WGO87_001814"
     CDS             complement(228..551)
                     /locus_tag="WGO87_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0393135.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(632..1288)
                     /locus_tag="WGO87_001815"
     CDS             complement(632..1288)
                     /locus_tag="WGO87_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0393136.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     ISDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNIVPDVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRQGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1391..1894)
                     /locus_tag="WGO87_001816"
     CDS             complement(1391..1894)
                     /locus_tag="WGO87_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0393137.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEYLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            2078..2851
                     /locus_tag="WGO87_001817"
     CDS             2078..2851
                     /locus_tag="WGO87_001817"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0393138.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2858..3208
                     /gene="secG"
                     /locus_tag="WGO87_001818"
     CDS             2858..3208
                     /gene="secG"
                     /locus_tag="WGO87_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0393139.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKYGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3222..3307
                     /locus_tag="WGO87_001819"
     tRNA            3222..3307
                     /locus_tag="WGO87_001819"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3256..3258,aa:Leu,seq:gag)
BASE COUNT         1018 a         1176 c          786 g          750 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaactttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg atactgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcaggc tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgccttca attccgccga cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaaa ccaagcaaaa tgccgtctga agccccaaac
      601 ccactttcag acggcatgac ctgtcaacat cttaaaaatc gaaaccgcca aacggatcgg
      661 catccttatc atccaaatgc gccaccaagg tatcgaacag caccttctct tcaaacacat
      721 cgccttgacg cgtaatcaaa agcgcgcgtt gaacaggctt gcggcctacg ataaccacca
      781 tgcgtccgcc atctttcaac tgttctttca acacatcagg cacaatattg accgcaccgc
      841 cgacataaac cgcatcaaac ggcgcacctg cggaaagttc ggtcagcccg ttgttttgca
      901 cataatcgat attgtccaaa cccaagccgt ccaacaccgc tttggcgcgg ttttgctgtt
      961 ccgcatcgat atcgtccgaa attaccctgc ccgccaattt tgccaacagc gcggtcgcat
     1021 agcccgaacc cgtgccgatt tccaaaaccg tatcgttttt cgtcagcttc aagccctgcg
     1081 ccagccgcgc cacgactttc ggctccagca tcttatgacc gttggcaagc ggcagcgcca
     1141 tatccgcata cgccaaaccc tgcaaatcct catcgacaaa aagctcgcgc ggaatctccg
     1201 ccaaagcgtc caaaacgtca aaatccaata catcccaagg acggatttgc tgttcgacca
     1261 tattgaaccg cgctttttca aaatccattt agtgctccgt caaataattg tttcaacaat
     1321 agcaggcatt ataaaaccac actcacgccg cgccaacttc ggcacgcgcc gacagcccgt
     1381 tttgtcagtc tcaagccgcc tgacgcgaag cctcaaaccg cttctccaaa atcttcgcca
     1441 gttcgcccaa atacaaagca tctgtctcat caaactgcgc caaatattcg ctgtccgcgt
     1501 ccaacacgcc gatacagcgg ccgtctgaaa acagcggcac gacaatctcc gaacgcgaca
     1561 aagacgaaca ggcaatatgg tcgggatgcg cgtccacatc cccgacgacc accgtcccgc
     1621 ccttcgccca agcctgaccg cacaccccgc gtccgaacgg aatccgcgta cacgccaaag
     1681 gtccctgaaa cggtgccaaa accaattcgt ccgaacgcgt atcgaccaaa taaaaaccca
     1741 cccaaaacca gccgaacgcc tccttcaaaa ccgccgccgt gttcgccaaa ttcgccaccc
     1801 aatccgtctc gtccgccacc acagactcaa tctgcggcaa cacctcccga taaagcgcgg
     1861 ccttgtccga agccgaaaaa tgaagcgcgt gcatcacatc tcctatagtt gcatacatat
     1921 caggcggcca ttataaaaca acctgcccga aacaacattc caaaccgccc ccgcagccgc
     1981 ccgccccaag ttgcgaaccc gccgcatatc cactaaactt cacattgcaa cgcgccacac
     2041 gcggcagaca gaaaaaacac gacacggagc aaaaaagatg tatcaccaaa tcggaatgtg
     2101 ggatcaaaaa tgggtcatcg gcaactggaa aatgaacggc cggctccaga acaacaattc
     2161 gctcatgcac cgcttccgca tcctccccac cgccgaacgc gtcctcatcg gactcgccgc
     2221 cccgaccgtt tacctgctgc aactgcacaa cgccatgcaa atcgtcttaa acaaccgcat
     2281 cctcacctgc gcccaagacg tgagccgctt ccccgacaac ggcgcgtaca ccggcgaagt
     2341 gtccgccgaa atgctcgccg acaccggcac agacatcgtc ctcatcggac actccgaacg
     2401 cagcctttat ttcggcgaaa aaaacgaaat ccaacgccgc aaaatggaaa acgtcctcaa
     2461 cgtcggactc atcccgttat tgtgcgtcgg cgaaagcctc gaagagcgcg aagccggcaa
     2521 agaacacgaa gtcatcgccc atcagctttc catcctgcaa ggactggaca ccaaaaacat
     2581 cgccgtcgcc tacgaacccg tctgggcgat cggcaccggc aaagtcgcca ccgtcgaaca
     2641 gattgccgat atgcacgcat tcatctacaa agaaatcttg tctttgtgcg gaagcgatgt
     2701 taaaatccgt gtcctttacg gcggaagtgt gaaagcggac aacgcggccg acatcttcgc
     2761 agtaccttat gtggacggcg cactcgtcgg cggcgcgtca ttgtcgtacg actcctttac
     2821 cgccatcatc agtgccgcac aaaatgcgta gaaaaatatg gaagccttca aaaccctaat
     2881 ttggattgtt aatataattt ccgctttggc cgtcatcgtg ttagtattgc tccaacacgg
     2941 caaaggcgcg gatgccggcg cgaccttcgg atcgggaagc ggcagcgcgc aaggcgtatt
     3001 cggctctgcc ggcaacgcta acttcctcag ccgttcgacc gccgttgcag caacattttt
     3061 ctttgcaacc tgcatggcta tggtgtatat tcacacccac acgacaaaat acggtttgga
     3121 cttcagcaac gtacaacaaa ctcagcaagc acccaaaccc gtaagcaata ccgaaccttc
     3181 tgcccctgtt cctcagcagc agaaataaca gtttttcaaa tgccgacatg gtgaaattgg
     3241 tagacacgct atcttgaggg ggtagtggcc gtaggctgtg cgagttcaaa tctcgctgtc
     3301 ggcaccaaca cacaaaacgc ttagaaattc taagcgtttt tttcgtttaa tcctgtctaa
     3361 tgccgtcaac caatcccaat caatacaagg atttcaggct gattgttatc ctgccgtccc
     3421 ccttcctgac agtgcaatcc cgtccaatcc gccctaattg aagtaaccta aaatttacgg
     3481 tatcttttgc ggtatctgaa aaatacctcg aaaaaatacc gcaaaaataa agctgaacga
     3541 ccgccaaatc aggaatgcca agcggaaaag agcttgcggg gaatactgcc aagacgtagg
     3601 gaacaagggg gaaaccgtcc aagatgcagg gcggtttttt ttgggttttt ggaaaaaacc
     3661 tatactagga agcgataccc ttagttgtta ccttgttacc ggggaaaagt tagataaata
     3721 agcatatgaa
//
LOCUS       DAWFBO010000169         3720 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFBO010000169 DAWFBO010000000
VERSION     DAWFBO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.169"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(88..906)
                     /locus_tag="WGO87_001820"
     CDS             complement(88..906)
                     /locus_tag="WGO87_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0393140.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTGLSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(973..2223)
                     /locus_tag="WGO87_001821"
     CDS             complement(973..2223)
                     /locus_tag="WGO87_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0393141.1"
                     /translation="MIKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVAADDPFIRNIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WGO87_001822"
     CDS             complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WGO87_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0393142.1"
                     /translation="MSDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGSESLPL"
     gene            2871..3683
                     /locus_tag="WGO87_001823"
     CDS             2871..3683
                     /locus_tag="WGO87_001823"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0393143.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          822 a          906 c         1116 g          876 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcgggaaa tatccatatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggag aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaatccg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggtgga agtaggttta tgcctgcctt catccgacta
      961 ccgccttttc aatcaacccc gcttcaatac ggatgtattg atgtagcgtt ggacacccga
     1021 ggcaatggat tgggcgcact gccggcggaa ggattcgctg cccagcaact tctcttcagc
     1081 agggttggac aggaaggcgg tttcgaccag gatagacggc atatcgggtg cgcgcaaaac
     1141 ggcgaaattg gcttcgtcca ccctgccttt gtgcaagtgg ttgagcctgc ccaattcttc
     1201 aagcaccagt ttgccgagtt tgcggctgtc gcgcagcgtg gcggtttggg tcatatcgag
     1261 cagggcggta tcgacgttgc ggttgccgct ggtcggtacg ccgccgaccg cgtcggcatt
     1321 gttttgcgtc tgttccaaga atttggcggc ggagctggtc gcgcctttgg tgttcagcat
     1381 atagaccccc gttccgcgcg cggaggggct ggtgaaagcg tcggcgtgga tggagacgaa
     1441 tacgtccgcc cgccgtgcgc gccctttggc gacacgcacg cccaaaggga tgaacacgtc
     1501 ttcgttgcgc gtcataaaga cgttgtaacc caaggcttcc aattgttttt tggtttcgcg
     1561 ggcgatggag aggacgacgt gtttttcctg caaaccgccc gggctgacgg cgccggggtc
     1621 ttcgccgccg tgtccggggt cgatcataat gacgggtctg cgcccgtttt tgccgcgttc
     1681 ggtttgtgca ggagtgtttt gagcgaggtc ggcttcaggg gagccgcgac gggttttgtt
     1741 caggctgccg ttaagcagag ccatcatcgg atcgtcggga tccatccctt gcggatagag
     1801 atcgacgacg aggcggtttt taaagccgcc gacggggggg agggaaaaaa cttgcgcgta
     1861 ggaaggctgt ttcaaatcga tgacgaggcg gacggtgtcg ggcgtgttct gaccggcgcg
     1921 tatattgcgg ataaaggggt cgtccgccgc cactttttgg gacagtccgt gcaatacggt
     1981 attgatgttt gcgttttgta tgtcgacgac cagcctgccc ggattgtcga gcgcgaagtg
     2041 ctggtatttg agcgcggcgg tgctttccag cgttaggcgg gtgtaggtgt gcgacggcca
     2101 tatccgtgcg gcggtgaatt gcggggcgcg taccgttttg gcaacggcgg atgcgatggg
     2161 gcttagggcg aacagtgtgc cggcggtgcg gcggatgatt tgtcttcgtg tcagtttgat
     2221 catagcggca ggctttcgct tcctcgttcg gtatgggcgg tcagcaggca ttttctgccg
     2281 ccgccgccgt gtgtcaatgt tgcggtgatg tcggcgggcg gcgtaaattc cccgccctgt
     2341 tgcggccatt cgatcaggca gacgctgttt gcggcaaaca gttcgtcaag ccccgcgtct
     2401 tcccattctt cggggaacga gaagcggtag aggtcgaaat ggtgcaaggt gaagcgttcc
     2461 agcggataag attcgacgat ggcgtaggtc gggcttttga ctgcgccctg atgacccaat
     2521 ccgcgcagga ttccgcgtgt cagcgtggtt tttcccgcac ccaaatcccc ttcgaggtaa
     2581 atgaccagcg gtgcgtttaa acgggaagac cacgccgcac ccaaatcgag tgtggcggct
     2641 tcgtcggcaa ggaatcggga aacgggagga aaatcagaca taggggcttt acaactttta
     2701 tgtggcggat cggaggggca ggcggtcgga aaaccgtgcg cccggcggta gcattgccgt
     2761 atattataac agccaaatcc gcatcctgct ttcagacggc aacggctgtc aagaaaaagc
     2821 ggcacgtgta caatacgcag attgtatgtt taggacggat tggaaaaagg atggaaaata
     2881 tcggcaggca gcgacccatc ggcgtttttg actcgggaat cggcggtttg accaatgtgc
     2941 gcgcgctgat ggagcggctg ccgatggaga acatcattta tttcggcgat acggcgcgcg
     3001 tgccttacgg gacgaaatct aaggcgacca tcgaaaattt ctcgatgcag attgttgatt
     3061 ttttattgga acacgatgtc aaggcgatgg ttatcgcgtg caacacgatt gcggcggtgg
     3121 cggggcagaa aatccgtcaa aagacgggca atatgccggt tttggacgtg atttccgccg
     3181 gcgcgaaagc cgcgctggca acgacgcgca acaacaaaat cggcattatc gccaccaata
     3241 cgacagtcaa cagcaacgcc tatgcgcgcg ccatccatag gaacaacccc gacacgcttg
     3301 tccgcacgca ggccgcgccg ctgctcgtcc ctttggtgga agagggctgg ttggaacacg
     3361 aagttacccg cctgaccgta tgcgaatacc tcaaaccatt gcttgcagac ggcatcgata
     3421 cgctggtgtt gggctgcacg cactttccct tgctcaagcc cttaatcggc agggaggcgg
     3481 gcaatgtcgc gttggttgat tctgcaatta caacggccga agaaaccgca cgcgtccttg
     3541 ctcaggaagg attgctcaat accgacaaca acaatcccga ctaccgtttt tacgtcagcg
     3601 atattccttt gaaattcaga accatcggcg agcgttttct gggcaggacg atggagcaga
     3661 ttgaaatggt gtctttgggt taaaacgatg acggaaagct gcccgagatt acagaaacct
//
LOCUS       DAWFBO010000170         3704 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFBO010000170 DAWFBO010000000
VERSION     DAWFBO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.170"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            262..585
                     /locus_tag="WGO87_001824"
     CDS             262..585
                     /locus_tag="WGO87_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0393144.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGTSAPDFNAGGTG
                     IGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPESAYRR
                     LYKPK"
     gene            599..3460
                     /locus_tag="WGO87_001825"
     CDS             599..3460
                     /locus_tag="WGO87_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ0393145.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTVIEGGSLVLYGNNESDMRVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAIIGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSITEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1003 a         1011 c          986 g          704 t
ORIGIN      
        1 agggaaattc aaattaagtt agaattatcc ctataagaaa aagccgtcta agccggtata
       61 aacaaaataa actcattgag ctatttgtcg aaagttcaaa tttccatttt aaaacaatta
      121 gtaaaatcga gtttatccta gttgtccaag acaaccccta taataatata attcaaaata
      181 taaaaatggg ttacatctaa acattacgga atttttattc cctcgcctga attctattgt
      241 cagattcaag gagacctcat catgcgaacg accccaacct tccctacaaa aactttcaaa
      301 ccggctgcca tggcgttagc tgttgcaaca acactttctg cctgcttagg cggcggcact
      361 tctgcgcccg acttcaatgc aggcggcacc ggtatcggca gcaacagcag ggcaacaaca
      421 gcgaaatcag cagcagtatc ttacgccggt atcaagaacg aaatgtgcaa agacagaagc
      481 atgctctgtg ccggtcggga tgacgttgcg gttacagaca gggatgccaa aatcaatgcc
      541 cccccccccg aatctgcata ccggagactt tacaaaccca aatgacgcat acaagaattt
      601 gatcaacctc aaacctgcaa ttgaagcagg ctatacagga cgcggggtag aggtaggtat
      661 cgtcgataca ggcgaatccg tcggcagcat atcctttccc gaactgtatg gcagaaaaga
      721 acacggctat aacgaaaatt acaaaaacta tacggcgtat atgcggaagg aagcgcctga
      781 agacggaggc ggtaaagaca ttgaagcttc tttcgacgat gaggccgtta tagagactga
      841 agcaaagccg acggatatcc gccacgtaaa agaaatcgga cacatcgatg tggtctccca
      901 tattattggc gggcgttccg tggacggcag acctgcaggc ggtattgcgc ccgatgcgac
      961 gctacacata atgaatacgc atgatggaac caagaacgaa ataatgtctg cagccatccg
     1021 caatgcatgg gtcaagctgg gcgaacgtgg cgtgcgcatc gtcaataaca gttttggaac
     1081 aacatcgagg gcaggcactg ccgaccattt ccaaatagcc aattcggagg agcagtaccg
     1141 ccaagcgttg ctcgcctatt ccggcggtga taaaacagac gagggtatcc gcctgatgca
     1201 acagagcgat tacggcaact tgtcctacca catccgtaat aaaaacatgc ttttcatttt
     1261 ttcggcaagc aatgacgcac aagctcagcc caacacactg accctattgc cattttatga
     1321 aaaagacgct caaaaaggca ttatcacagt cgcaggcgta gaccgcagtg gagaaaagtt
     1381 caatggctcc aaccattgcg gaattactgc catgtggtgc ctatcggcac cctatgaagc
     1441 aagcgtccgt ttcacccgta caaacccgat tcaaattgcc ggaacatcct tttccgcacc
     1501 catcgtaacc ggcacggcgg ctctgctgct gcagaaatac ccgtggatga gcaacgacaa
     1561 cctgcgtacc acgttgctga cgacggctca ggacatcggt gcagtcggcg tggacagcaa
     1621 gttcggctgg ggactgctgg atgcgggtaa ggccatgaac ggacccgcgt cctttccgtt
     1681 cggcgacttt accgccgata cgaaaggtac atccgatatt gcctactcct tccgtaacga
     1741 catttcaggc acgggcggcc tgatcaaaaa aggcggcagc caactgcaac tgcacggcaa
     1801 caacacctat acgggcaaaa ccgttatcga aggcggttcg ctggtgttgt acggcaacaa
     1861 cgaatcggat atgcgcgtcg aaaccaaagg tgcgctgatt tataacgggg cggcatccgg
     1921 cggcagcctg aacagcgacg gcattgtcta tctggcagat accgaccgat ccggcgcaaa
     1981 cgaaaccgtg cacatcaaag gcgatctgca gctgggcggc gaaggtacgc tgtacacacg
     2041 tttgggcaaa ctgctgaaag tggacggtac ggcgattatc ggcggcaagc tgtacatgtc
     2101 ggcacgcggc aaaggggcag gctatctcaa ccgtaccgga caacgtgttc ccttcctgag
     2161 tgccgccaaa atcgggcggg attattcttt cttcacaaac atcgaaaccg acggcggtct
     2221 gctggcttcc ctcgacagcg tcgaaaaaac agcgggcagt gaaggcgaca cgctgtccta
     2281 ttatgtccgt cgcggcaatg cggcacggac tgcttcggca gcggcacatt ccgcgcccgc
     2341 cggtctgaaa cacgccgtag aacagggcgg cagcaatctg gaaaacctga tggtcgaact
     2401 ggatgcctcc gaatcatccg caacacccga gacggttgaa actgcggccg ccgaccgcac
     2461 agatatgccg ggcatccgcc cctacggcgc aactttccgc gcagcggcag ccgtacagca
     2521 tgcgaatgcc gccgacggtg tacgcatctt caacagtctc gccgctaccg tctatgccga
     2581 cagtaccgcc gcccatgccg atatgcaggg acgccggctg aaagccgtat cggacgggtt
     2641 ggaccacaac gctacgggtc tgcgcgtcat cgcgcaaacc caacaggacg gtggaacgtg
     2701 ggaacagggc ggtgttgaag gcaaaatgcg cggcagtacc caaaccgtcg gcattgccgc
     2761 gaaaaccggc gaaaatacga cagcagccgc cacactgggc atgggacaca gcacatggag
     2821 cgaaaacagt gcaaatgcaa aaaccgacag cattagtctg tttgcaggca tacggcacga
     2881 tgcgggcgat atcggctatc tcaaaggcct gttctcctac ggacgctaca aaaacagcat
     2941 cagccgcagc accggtgcgg acgaacatgc ggaaggcagc gtcaacggca cgctgatgca
     3001 gctgggcgca ctgggcggtg tcaacgttcc gtttgccgca acgggagatt tgacggtcga
     3061 aggcggtctg cgctacgacc tgctcaaaca ggatgcattc gccgaaaaag gcagtgcttt
     3121 gggctggagc ggcaacagca tcactgaagg cacactggtc ggactcgcgg gtctgaagct
     3181 gtcgcaaccc ttgagcgata aagccgtcct gtttgcaacg gcgggcgtgg aacgcgacct
     3241 gaacggacgc gactacacgg taacgggcgg ctttaccggc gcgactgcag caaccggcaa
     3301 gacgggggca cgcaatatgc cgcacacccg cctggttgcc ggtctgggcg cggatgtcga
     3361 attcggcaac ggctggaacg gcttggcacg ttacagctac gccggttcca aacagtacgg
     3421 caaccacagc ggacgagtcg gcgtaggcta ccggttctga cggacaggaa gcagacagcc
     3481 gcaaagatca cggtctttgc ggctgtttct tatgaaaaga aaaccctatt ccaattgcct
     3541 gcttctattg tttcaagact tcttccaaag attcggcatc aatcagatgt atagcggatt
     3601 aacaaaaatc aggacaaggc ggcgaagccg cagacagtac agatagtacg aaaccgattc
     3661 actcggtgct tcagcacctt agagaatcgt tctctttgag ctaa
//
LOCUS       DAWFBO010000171         3703 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFBO010000171 DAWFBO010000000
VERSION     DAWFBO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.171"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..414)
                     /locus_tag="WGO87_001826"
     CDS             complement(<1..414)
                     /locus_tag="WGO87_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ0393146.1"
                     /translation="MTHISSPRNEFIRGIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAESLPILLIATVTFMINSRHILMGAALALHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
     gene            559..1011
                     /gene="dut"
                     /locus_tag="WGO87_001827"
     CDS             559..1011
                     /gene="dut"
                     /locus_tag="WGO87_001827"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0393147.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGSH"
     gene            1089..2276
                     /gene="dapC"
                     /locus_tag="WGO87_001828"
     CDS             1089..2276
                     /gene="dapC"
                     /locus_tag="WGO87_001828"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0393148.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDFQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLKRGREKLVMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            2538..3317
                     /gene="yaaA"
                     /locus_tag="WGO87_001829"
     CDS             2538..3317
                     /gene="yaaA"
                     /locus_tag="WGO87_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0393149.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3400..3490
                     /locus_tag="WGO87_001830"
     tRNA            3400..3490
                     /locus_tag="WGO87_001830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3434..3436,aa:Ser,seq:tga)
     gene            3568..3657
                     /locus_tag="WGO87_001831"
     tRNA            3568..3657
                     /locus_tag="WGO87_001831"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3602..3604,aa:Ser,seq:cga)
BASE COUNT          971 a          864 c          911 g          957 t
ORIGIN      
        1 aggcatattg aatgcgggca aaccggctgc tttccgtttt tggatttcgg agaatgccat
       61 cgcccagctt tcatcacaca taaaaaacag tgcgggcacg gcttttttca gcggtatttc
      121 tttcaggtgc agggcaagtg ccgcccccat caggatatgc cgcgaattaa tcataaaggt
      181 tacggtggcg ataagcagta tcggcagaga ttccgcccac aggttgaccg tggcaaactc
      241 ggagccgccg gcgaagttca tactggtcat caacaacatt tccagccagc tcatgccttt
      301 ttgtccgccc tgcataccga gtattaatgc ccaaggcaga agtccgataa gtatgggcga
      361 actttctttg atgccgcgta taaattcgtt acggggcgag cttatgtgtg tcatattata
      421 atattgatat gtaagaaggt cgggcattat acacagacgg agtatttcaa aacaaaaccg
      481 atttgccgtg tttcagcgta aacacggctt gtgtataatc tcccatcttt aaaaccggcc
      541 gtatgcagga gcaagatgat gaatattgaa gtagaaatga aagtgttgga cgagaggatg
      601 gcggatttta tccctgccta tgcgacggag ggttctgctg gtttagattt gcgtgcctgt
      661 ttggatgagg aagttgtctt gcagccgggt gaaacgtttc ttgtaccgac aggtttggca
      721 atttatttgg cgaatcccgc atatgccgcc gttttgctgc cccgttccgg attggggcat
      781 aaacacggca ttgttttggg caatttggtc ggcttgattg actccgatta tcaaggggaa
      841 ttgaaggttt cgttatggaa cagaagcagc gagccgttta ctgtcaaacc gtttgagcgt
      901 atcgcgcaga tggttatcgt gccaatcgtg caggcgcgtt tcaaacgtgt cgaggaattt
      961 gtcggaagca gccggggaga aggcggcttc ggcagtacgg gttctcacta aaaatataga
     1021 atgccgtctg aaagacacgt caggttcaga cggcatatct tccgatatac cgacaaccgg
     1081 agaaaatcat gaatacctta ctcaaccaac tcaaacccta tccctttgcc cgactgcgtg
     1141 aagcgatgca gggcatttcc gcccccgaag gtctggaagc cgtcccactg catatcggcg
     1201 aaccgaaaca tccgacaccg aaagtcatta cggatgcgct gaccgcctca ttgcacgagt
     1261 tggaaaaata tccgctgacg gccggtttgc ctgaactgcg tcaggcgtgt gcgaactggt
     1321 taaaacgccg ttatgatggg ttgacgctga attccgacac cgaaattctg ccggttttgg
     1381 gaagtaggga ggcgttgttt tcttttgtcc aaaccgtgtt gaaccctgtt tcagacggca
     1441 tcaaacccgc aattgtcagc ccgaatccct tttatcagat ttacgaaggt gcgacacttt
     1501 tgggcggcgg tgaaatccat tttgccaatt gccccgcgcc gtctttcaac cccgattggc
     1561 gtagtatttc cgaagaggtt tggaaacgca ccaaactggt gttcgtctgc tcgcccaaca
     1621 accccagcgg cagcgtgctg gatttggacg gctggaaaga agtttttgat tttcaagaca
     1681 aatatggttt cattattgcc tcggatgaat gctattccga aatctatttc gacggtaaca
     1741 aacctttagg atgtctgcaa gctgcagcgc aattgaaacg gggcagggaa aaactggtta
     1801 tgtttaccag tttgtccaag cggtccaatg ttccaggcct gcgttccggt tttgtcgccg
     1861 gcgatgccga actgcttaaa aattttctgc tttacagaac ctatcacggc agtgcaatga
     1921 gcattcccgt gcagcgcgca agcattgccg cttgggatga cgaacagcac gttatcgaca
     1981 accgccgcct gtatcaggaa aaatttgagc gcgttattcc cattttgcaa caggtatttg
     2041 acgttaaatt accggatgcc tcgttttaca tctggttgaa agtccccgat ggcgacgatt
     2101 tggcatttgc acgcaattta tggcaaaaag ccgctatcca agtattgccc ggacgctttt
     2161 tggcacggga taccgaacag ggcaatcccg gggaaggtta tgtccgtatc gctttggttg
     2221 ccgatgtcgc aacttgtgtc aaagctgcgg aagacatagt ttcactgtat cggtaaggaa
     2281 tcggaaatgc cgtctgaact tttgttcaga cggcattttt caatattcta cggttgaatt
     2341 tgctgtaacg gtatttatag tggattaaca aaaatcagga caaggcgacg aagccgaaga
     2401 cagtacagat agtacggcaa ggcgaggcaa cgctgtactg gtttaaattt aatccactat
     2461 acttcacttt taatcggctt gcccgcaaac acgtttaaac ttaaaatccc cgtgtttgac
     2521 acaataccga gcagattatg ttttttgtcc tttcccctgc gaagaacctt aatgaaaaag
     2581 accctgcccc tgtcagcgag tttacccaac ccgacctgct ggcagagtcc gacattctaa
     2641 tgcagcagtt gcgcgagctt gcgccgcaac agattgccga actgatgcac gtttccgaca
     2701 aaattgccct cttaaacgcg cagcgcaatg cagaatggaa cacgccgttt acgccggaaa
     2761 acgccaaaca ggcggtcttt atgttcaacg gcgatgttta cgaaggtatg gatgcaaaca
     2821 cattggatat tggacagata cgctatctgc aaaaccatgt ccgcctgctg tccggtctgt
     2881 acggtcttct tcgcccgtta gacctgatac agccctaccg tttggaaatg gggacggcat
     2941 ttgccaattt gcgcggcaag aatttgtatg agttttgggg cgacatcatt accaaccttt
     3001 taaatgacac gcttgcccaa gcaggcagca atacgcttgt caaccttgcc tcacaggaat
     3061 atttcaagtc cgtcaacacg aaaaaacttc gggcgcggct gattaccccg atatttaaag
     3121 acgaaaaaaa cggtaaatat aaaatcatca gtttctatgc caagcgtgcg cgtggattaa
     3181 tggtgcgcta tgcggcagaa catcatatta ccgaccctga aatgctgaaa aattttaatt
     3241 acgaaggcta cgcattcaat gacgcggctt caaatgaaag cgaatgggtt tttatgcgtt
     3301 cggaacaaat aaagtgaaaa caataaatta agtattttcc gaaaaaagtg cttggcaaaa
     3361 tgtataaatt tcattattat tcctaatctt caagaagacg gaagcgtggc agagcggttt
     3421 aatgcaacgg tcttgaaaac cgtcgagggt tgatagccct ccgtgagttc gaatctcacc
     3481 gcttccgcca atccttgagg tcaaaaacca aataaaaatg aagtagcata aagcatcggc
     3541 atttttattt ggttttactg cattatcgga aacgtggcag agaggctgaa tgcagcggac
     3601 tcgaaatccg ctgagggtgc aaatcctccg tgggttcgaa tcccaccgtt tccgccacaa
     3661 aacaaaaccg ccctgattcg gggcggttct tttttgttca agt
//
LOCUS       DAWFBO010000172         3691 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFBO010000172 DAWFBO010000000
VERSION     DAWFBO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.172"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            448..3417
                     /locus_tag="WGO87_001832"
     CDS             448..3417
                     /locus_tag="WGO87_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0393150.1"
                     /translation="MNEGNQPEVQWNGSYSIKDKDRKREYTHHNHSRGGSSVSFNNSD
                     ELVSQQSGTAVFGTATYLPPYGKVSGFDADGLNKRGNAAGWIRTTRIALAGYSYEGIV
                     CRSGTGCPKLVYKTRFSFDNPDLVKNAGRLDRHTDPSRENSPIYKLKDYPWLGVSFNL
                     GAEGTTKDGKTINKLVSSFDEKNSSNNNLVYTTEGRDISLGDWQREKTAMAYFLNAKL
                     HLLDKKGIKDITNKTVQLGVLRPSIDVRLQRNTGLAGLLNFWASWDIKDNGQIPVKLG
                     LPEVKAGRCINANNPNKSTKAPSPALTAPALWFGPVQNGKMEMYSASVSTYPDSSSSR
                     IFLQNLKRKNDPNKPGRYSLATLNKSDIESREPTFTGRQTVIRLDKGVHQIKLKGNEV
                     EGFKGNNGNDTFGIVSEGSFMPDDSEWKKVLLPWTVRGVNDDQFKTFNKEEKNGKPKY
                     SQKYRSRDNGKHERNLGDIVNSPIVAVGEYLATSANDGMVHIFKKGNGDARNYSLKLS
                     YIPGTMPRKDIQNTESTLAKELRAFAEKGYVGDRYGVDGGFVLRQVNNLNGQDRVFMF
                     GAMGFGGRGAYALDLTKADSNNPTAVSLFDVKNDKNKGNNSAELGYTVGTPQIGKTHD
                     GKYAAFLASGYATKTIDDQQNKTALYVYDLESSGTLIKKIDVPGGKGGLSSPTLVDKD
                     LDGTVDIAYAGDRGGNMYRFDLSGQDPNQWSVRTIFSGNKPITSAPAISQLKDKRVVI
                     FGTGSDLSEEDVDNNDIQSIYGIFDNDTDTGTAQDGQGNGLLEQVLKKDGNTLFLSDY
                     KRSNGSGDKGWVVKLEAGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAETAILGINTAD
                     GGKLTKKSARPIVPEANQAVAQYSGHKKGTNGKSIPIGCMWKNNETVCPNGYVYDKPV
                     NVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDLDSLDI
                     TGPTCGMKRISWREIFY"
BASE COUNT         1027 a          928 c          972 g          764 t
ORIGIN      
        1 ctgtcgggca tctgcagcgg tttgctaaaa aacgctttac cgtgatcagt gtgcaaagtt
       61 aaaatgggga ggtaagcttt tcaatcagca atccggcggg cgcgggatcg ggcggtttgc
      121 cgaatcccgc cgtttgcggc gcgcctgccg ccgacggtat cccgcgaagc aagatttaag
      181 ggataaaata tgttccaaca cgcagggcgg cacataaggc gccgccctga ttcggaaggg
      241 gctgcatccc tcccggacaa agcctgatcc tgccgccccg aaggacggat gcccgaaggg
      301 cggggggttt gaccgaaaag gaaatacgat gaataaaact ttaaaaaggc gggttttccg
      361 ccataccgcg ctttatgccg ccatcttgat gttttcccat accggcgggg gggggcgcag
      421 gcgcaaaccc gtcaatacgc tattatcatg aacgagggaa accagcccga ggtacagtgg
      481 aatgggtcat attcaataaa ggacaaagac aggaagcgcg aatatactca tcataatcac
      541 tcacgaggag gaagctctgt ctcattcaac aatagcgatg agcttgtttc tcaacaaagc
      601 ggtactgccg tttttggcac agccacctac ctgccgccct acggcaaggt ttccggtttt
      661 gatgccgacg ggctgaacaa gcgcggcaat gccgccggtt ggattcgtac cacccgcatc
      721 gcgctggcag gctacagcta cgaaggtatc gtatgcagaa gcggcacagg ctgtcccaaa
      781 cttgtctata aaacccgatt ttccttcgat aatcccgact tggtaaaaaa tgcgggcagg
      841 ctggataggc acacagaccc aagccgcgaa aattcgccca tttacaaatt gaaggattat
      901 ccatggttgg gcgtatcttt caatttgggt gccgagggta ccaccaaaga tggtaagaca
      961 atcaacaaat tggtatcttc ttttgatgaa aagaatagta gtaataacaa cctcgtctat
     1021 accacggaag gccgcgatat ttccttgggc gactggcagc gcgaaaagac cgccatggcc
     1081 tattttctga acgccaaact gcacctgctg gacaaaaaag ggattaaaga tatcaccaac
     1141 aaaacagtgc agttgggtgt cttgaggccg agcatcgatg tgcggctgca aagaaatacg
     1201 gggcttgccg gcttgctaaa tttttgggct agttgggaca ttaaagataa cgggcagatt
     1261 ccggtcaagc tcggcctgcc ggaagtcaaa gcaggccgct gcatcaacgc aaataacccc
     1321 aataagagta ccaaagcccc ttcccccgca ctgactgccc ccgcgctgtg gttcggacct
     1381 gtgcaaaatg gtaagatgga gatgtattcc gcttcggttt ctacctaccc cgacagttcg
     1441 agcagccgca tcttccttca aaatctgaaa agaaaaaacg accccaacaa acccggccgc
     1501 tattccctcg caaccttgaa taagtcggat attgaaagtc gagagccgac tttcacaggg
     1561 cggcaaaccg tcatccgatt ggataaaggc gtacatcaga tcaaacttaa aggcaatgag
     1621 gtcgaaggtt ttaagggaaa caacggcaac gacactttcg gcattgttag tgaagggagc
     1681 ttcatgcctg atgacagcga gtggaaaaaa gtgctgctgc cttggacggt tcggggtgtc
     1741 aatgacgatc aatttaaaac attcaacaaa gaagaaaaaa acggcaagcc aaaatacagt
     1801 caaaaatacc gcagccgcga caacggcaag cacgagcgca atttgggcga catcgtcaac
     1861 agccccatcg tggcggtcgg cgagtatttg gctacttccg ccaacgacgg gatggtgcat
     1921 atcttcaaaa aaggcaacgg ggacgcgcgc aactatagtc tgaagctcag ttatatcccg
     1981 ggcacgatgc cgcgcaagga tattcaaaac accgaatcca cccttgccaa agagctgcgc
     2041 gcctttgccg aaaaaggcta tgtgggcgac cgctacggcg tggacggcgg ctttgtcttg
     2101 cgccaagtta acaacttaaa tgggcaagac cgcgtgttta tgttcggcgc gatgggcttt
     2161 ggcggcagag gcgcatacgc cttggatttg accaaagccg acagcaataa cccgaccgcc
     2221 gtttccctgt ttgatgtcaa aaatgacaaa aataaaggca ataatagtgc agaattaggc
     2281 tacaccgtcg gcacaccgca aatcggcaaa acccacgacg gcaaatacgc cgctttcctc
     2341 gcctccggtt atgcgactaa aaccattgac gaccaacaaa ataaaaccgc gctgtatgtg
     2401 tatgatttgg aaagcagcgg tacgctgatt aaaaaaatcg atgtacccgg cggcaagggc
     2461 gggctttcgt cccccacgct ggtggataaa gatttggacg gcacggtcga tatcgcctat
     2521 gccggcgatc gcggcgggaa tatgtaccgc tttgatttga gcggtcaaga tcctaatcaa
     2581 tggtctgtac gcaccatttt cagcggcaac aaaccgatta cttccgcacc cgccatttcc
     2641 caactgaaag acaaacgcgt ggttatcttc ggtacgggca gtgatttgag tgaggaagat
     2701 gttgataata atgatataca atctatttac ggtatttttg acaatgacac agacacgggt
     2761 acggcgcaag acgggcaggg caacgggctg ctcgagcaag tgctgaaaaa agacggcaat
     2821 acgctgttct taagcgacta caaaaggtct aacggctcgg gcgataaggg ctgggtagtg
     2881 aaattggaag ccggacagcg cgttaccgtc aaaccgaccg tggtattgcg taccgccttt
     2941 gtaaccatcc ataaatatac gggtacggac aaatgcggcg cggaaaccgc cattttgggt
     3001 atcaataccg ccgacggcgg caagctgacc aagaaaagcg cgcgcccgat tgtgccggaa
     3061 gccaatcagg ctgtcgcgca atattccggt cataagaaag gcaccaacgg caaatccatc
     3121 cctataggtt gtatgtggaa aaacaatgaa accgtctgcc cgaacggata tgtttacgac
     3181 aaaccggtta atgtgcgtta tctggatgaa aagaaaacag acggattttc aacaacggca
     3241 gacggcgatg cgggcggcag cggtatagac cccgccggca agcgttccgg caaaaacaac
     3301 cgctgcttct cccaaaaagg ggtgcgcacc ctgctgatga acgatttgga cagcttggac
     3361 attaccggcc cgacgtgcgg tatgaaacga atcagctggc gtgaaatctt ctactgattt
     3421 gcacgcgaaa atgccgtccg aaaggttttc ggacggcatt ttttgcgttt ttcgggaggg
     3481 gcgggttcgt aaaaggcggg ctatagggta ggcttcatct cgccaatctc actgaatcca
     3541 tcaatttcca caattcaatt aaataccgtc aaaccgatgc cgtcattccc gcgcaggcgg
     3601 gaatctagac cttagaacaa cagcaatatt caaagattat ctgaaagtcc gggattctgg
     3661 attcccactt ccgtgggaat gacgggatta g
//
LOCUS       DAWFBO010000173         3392 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFBO010000173 DAWFBO010000000
VERSION     DAWFBO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.173"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            43..708
                     /locus_tag="WGO87_001833"
     CDS             43..708
                     /locus_tag="WGO87_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0393151.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTVNNKCDSGKKGQGMLAFGDKNGNK
                     TYDGDAADVFLRSVVLNDTDDSRINYAFNHIAFGSSQPTADRVVWTFNQNGTFGYTKD
                     QHLTSKSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRNNRRTVC
                     QYK"
     gene            738..1352
                     /gene="pilV"
                     /locus_tag="WGO87_001834"
     CDS             738..1352
                     /gene="pilV"
                     /locus_tag="WGO87_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0393152.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSVVDGDFQVDA
                     IKTKTQLAEAQLKRFSYELKNALPDAAAIYYAICKDSSGVAPTLSAGSTFSSNCDASA
                     NGDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            1349..2290
                     /locus_tag="WGO87_001835"
     CDS             1349..2290
                     /locus_tag="WGO87_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0393153.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLSAQQDLRNAATLIVRDARMAGGFGCFNMSEHLATDVVSDVAQKNRL
                     FSLKRNSTNKLIPITESSNINYQNFFQVGSALIFQYGIDDVNASAATTVVSSCAAILK
                     PGKQILTLENVKKELNIPDTDNRQNGNIARQRHVVNAYAVGRIADEEGLFRFQLDDKG
                     KWGNPQLLVKKVRHMKVRYIYVSGCPEDDDAGKEETFKYTDKFDSSTNAVTPAGVEVL
                     LSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2269..2865
                     /locus_tag="WGO87_001836"
     CDS             2269..2865
                     /locus_tag="WGO87_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0393154.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCAKGLCTAVNVRT
                     NNTGNEEAFDNIVVQDNPTVEAVKRSCPANSTDLCIDKKGMKYKKGTRSVSEMPRYII
                     EYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2870..3343
                     /locus_tag="WGO87_001837"
     CDS             2870..3343
                     /locus_tag="WGO87_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0393155.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNTVKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAKKYSVSVKFVDKEKSRA
                     YRLVGVPKAGTGYTLSAWMNSVGDGYKCRDAASARAYSETLSADAGCEAFSNRKK"
BASE COUNT          957 a          684 c          862 g          889 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacggtca acaataaatg tgactccggc
      301 aagaaggggc agggaatgtt ggcttttggc gataagaatg gcaataagac atatgacggc
      361 gatgcggcgg atgttttcct ccgcagcgtg gtgttgaatg ataccgacga cagccggatt
      421 aattatgcct tcaaccatat cgctttcggt tcgtctcagc cgaccgccga ccgtgtagtt
      481 tggacattca atcaaaacgg gacgttcggt tatacgaaag accagcatct tacaagcaaa
      541 tccagctttt tttattccga cggttatatc caaatcgtgc tgacagatgc gagagcagtt
      601 tcagatgccg ataagaaatt ccgttcggcg gtggttttga ttgacagcag cggcagggtt
      661 gaggtttgtc ctagaaacaa taggcgtacc gtatgccaat ataaatgatt gttttagtcg
      721 gtttgggaaa taaaaaaatg aagaataatg attgcttccg cctgaaaaac ccccagtccg
      781 gtatggcgct gatagaagtc ttggtcgcta tgctcgttct gaccatcggt attttggcac
      841 tattgtctgt tcagttgcgg acagtcgctt ccgtcaggga ggcagagaca caaaccatcg
      901 tcagccaaat cacgcaaaac ctgatggaag gaatgttgat gaatccgacc attgattcgg
      961 acagcaacaa gaaaaactat aatctttaca tgggaaacca tcatacacta tcagttgtgg
     1021 atggcgattt tcaggttgat gccataaaaa ctaagacgca gttggcagag gcacaattga
     1081 agagatttag ttatgagctg aaaaatgcct tgccggatgc ggcagccatc tattacgcca
     1141 tctgcaagga ttcgtcgggt gttgcgccga cattgtccgc cggcagtact ttttcttcaa
     1201 attgcgatgc tagtgcaaat ggggatactt tgattaaagt attgtgggta aatgattcgg
     1261 caggggattc ggatatcgcc cgtacgaatc ttgagacgaa cggcaacaat atcgtatata
     1321 cctatcaggc aagggtcgga ggtcgggaat gagacgtaaa atgctaaacg taccaaaagg
     1381 cagttatgat ggtatgaagg gttttaccat tattgaattt ttggttgcgg gcatgctcag
     1441 tatgattgtc ctgatggcgg tcggatcgag ttacttcaca tcccggaaat taaatgatgc
     1501 ggcaaacgag cgtctttccg cgcaacagga tttgcggaat gcggcaacat tgattgtccg
     1561 cgatgcgaga atggcgggcg gcttcggttg tttcaatatg tccgagcatc ttgcaactga
     1621 tgttgtttcc gatgtggcgc aaaaaaaccg tcttttttcc ttaaaaagga acagtacaaa
     1681 taaacttatc cccataacgg aatcttcaaa tatcaattat cagaattttt tccaggttgg
     1741 tagcgcattg atttttcaat acggaatcga tgatgttaat gcaagcgccg cgactaccgt
     1801 cgtcagcagc tgtgccgcaa tattgaaacc gggcaagcaa atccttactt tagaaaatgt
     1861 aaaaaaagaa ttgaatattc cggatacgga taacaggcaa aatggcaata ttgcgcgtca
     1921 aaggcatgtg gtcaatgcct atgcggtcgg caggattgcc gatgaggaag gtttgttccg
     1981 cttccaattg gatgataagg gcaagtgggg taatcctcag ttgctcgtga aaaaggttag
     2041 acatatgaaa gtgcggtata tctatgtttc cggctgtcct gaagatgacg atgccggcaa
     2101 agaggaaaca ttcaaatata cggataaatt cgacagctcc acaaatgctg ttacgcccgc
     2161 cggggtggag gttttattga gtagcggtac tgataccaag attgccgctt cttcagacaa
     2221 tcatatttat gcttaccgta tcgatgcgac aatacgcggg ggaaatgtat gcgcaaacag
     2281 aacactttga cgggaatccc gacttctgac ggacagaggg ggttcgcgct gtttatcgta
     2341 ctgatggtga tgatcgtcgt ggcttttttg gttgtaactg ccgcgcagtc ttacaatacc
     2401 gagcagcgga tcagtgccaa cgaatcagac aggaaattgg ctttgtcttt ggccgaggcg
     2461 gctttgcggg aaggcgaact tcaggttttg gatttggaat atgatacgga cagtaaggtt
     2521 acatttagcg aaaactgtgc aaaaggtctg tgtaccgcag tgaatgtgcg gacaaataat
     2581 actggtaatg aagaggcttt tgacaatatc gtggtgcaag acaatcccac cgttgaggcg
     2641 gtgaagcgtt cttgccctgc aaattctacc gacctgtgca ttgacaagaa agggatgaaa
     2701 tataagaaag gcacgagaag cgtcagcgaa atgccacgtt atattatcga atatttgggc
     2761 gtgaagaacg gagaaaatgt ttatcgggtt actgccaagg cttggggtaa gaatgccaat
     2821 accgtggtcg tccttcaatc ttatgtaagc aataatgatg agtaataaaa tggaacaaaa
     2881 agggtttaca ttgattgaga tgatgatagt cgtcgcgata ctcggcatca tcagcgtcat
     2941 tgccatacct tcttatcaaa gttatattga aaaaggctat cagtcccagc tttatacgga
     3001 gatggtcggt atcaacaata ctgtcaaaca gtttattttg aaaaatcccc tggacgataa
     3061 tcagaccatc aagagcaaac tggaaatatt tgtctcaggc tataagatga atccgaaaat
     3121 tgccaaaaaa tatagtgttt cggtaaagtt tgtcgataag gaaaaatcaa gggcatacag
     3181 gttggtcggc gttccgaagg cggggacggg ttatactttg tcggcatgga tgaacagcgt
     3241 gggcgacgga tacaaatgcc gtgatgccgc ttctgcccga gcctattcgg agactttgtc
     3301 cgcggatgcc ggctgtgaag ctttctctaa tcgtaaaaaa tagggctgtt ttgccaatgc
     3361 cgtctgaaaa tcaatgttca gacggcattt tt
//
LOCUS       DAWFBO010000174         3373 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFBO010000174 DAWFBO010000000
VERSION     DAWFBO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.174"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(200..580)
                     /locus_tag="WGO87_001838"
     CDS             complement(200..580)
                     /locus_tag="WGO87_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0393156.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPSVQNQARPADF
                     REFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1056..3137
                     /locus_tag="WGO87_001839"
     CDS             1056..3137
                     /locus_tag="WGO87_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0393157.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3127..3321
                     /locus_tag="WGO87_001840"
     CDS             3127..3321
                     /locus_tag="WGO87_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0393158.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          740 a          997 c          861 g          775 t
ORIGIN      
        1 atcattggat aacagccctg aagaaatcgg agcaggttta aagttggcat tgagccgctg
       61 ccgataggat aaacccggaa cgaaacgaaa aataatccgc cacgcccgaa aaccgccgaa
      121 accagccgac agccaaaaca aatccgacac aaaaaaccgc ccgaaaaatc cggacggcgg
      181 ttcaaacagg ctgccccgtt tagcgggcgc ggcaggaagt ttcgaccgaa ttgccgtagg
      241 catcggtaaa gccgaaaaag gcttcgccgc ctttctggtg ccactcggtt gcgtttccga
      301 acaaaccgtg ttcggcggta tagcgttcgc cggatgcggc aacgtcggaa gagaggacgg
      361 cacgcctgcc gtccagccgc aacgcgactt tgccgctgtc caaatggcgg acgcgcacag
      421 acaaaccgtt ctcgcatgaa aactcccgaa aatctgccgg gcgggcttgg ttttgaacgg
      481 acggcatatg cccgcgtccg ccgtcatcat acgcctccgg cacggcacag gccgccaaag
      541 acaaaaccgg tacggtcagc gcgaaaaacc tgatattcat aaaagctccc caataaaaat
      601 aagatatgaa acaaccgccc tgattccaag ctgcggcaac gccatactat aaacggacgc
      661 gcaaacacac aagcccgata accggaattt acccgcgatg aatcaataat ccggattgcg
      721 cgccccttct ttacccgtct tccggcgccg ccttttgcct gacgatgccg tccaaacccg
      781 cctgtccgcc cgaaggaatg taaatttttt ccaaattcca agtaaaaacc gctatcagtg
      841 tgctaatttg cgttaaaatc ctattcggcg tttaacgttt tgtgcgcccg catccctgca
      901 ctgtttgatg cgggcataag gcacaaatcc cgacaagcgc actgtttcat acttcgtcaa
      961 tcattcagac tccggtttgt gcccgtgccg gcaggcggtc atccgtttcc cgccgttcag
     1021 gcatattccg acagtgtgag ataaggattt attcgatgaa atcactcaaa accttcctca
     1081 tttggggcat agtggtactg gtcggcttag catcctttac cactctggct ctcagccgag
     1141 gcgaacaggt cagcgcggta tggatggtta ccgccgccat atccgtttac tgcatcgcct
     1201 accgttttta cagcctctac atcgccaacc gcgtaatgcg gctcgatcct aaccgcctga
     1261 ctccggcaga acgccacaac gacggcttgg actacgttcc gacgcacaaa ggcgtattgt
     1321 tcggacacca ctttgccgcg attgcgggcg cgggcccttt ggtcggcccg gttttggcgg
     1381 cgcaaatggg ttacctgccc ggtactttgt ggattatctt cggcgtggtg tttgccggcg
     1441 cggtacagga tatgatggtc ttgttcgtct ctatgcgccg cgacggtaag tctttgggcg
     1501 atattgtgaa acaggaactc ggcactgtcc ccggcgtgat tgcctccatc ggtattttga
     1561 tgattatggt catcattatg gcggtgttgg cgttgattgt cgtaaaagca ctggttcaca
     1621 gcccttgggg tacgtttacc atcgccgcca ctatgccgat tgccctgttt atgggtattt
     1681 acacgcgcta tatccgtccg ggcaaaatcg gcgagatttc catcgtcggc tttattttgc
     1741 tgatgctggc ggtaatttac ggcgaagatg tggcgaaaag ttccatcgga cattggttcg
     1801 accttgacgg catccagctc acttgggcga ttatgattta cggctttgtc gcctccgtat
     1861 tgcccgtatg gttgctgctc actccgcgcg actatctctc caccttcctg aaaatcggta
     1921 cgattgcggc cttggctttg ggcatcgtca tcgtcaatcc cactttgcaa atgcctgccg
     1981 taacccactt tatcgacggt tcgggtccgg tattctcagg cgcattgttc ccgttcctct
     2041 tcatcaccat cgcctgcggt gcggtttcgg gcttccacgc gctgatttcc tccggcacca
     2101 cgccgaaaat gctggaaaac gaaacccacg tccgtatgat tggttacggc ggtatgttga
     2161 tggaaagttt cgtagccatt atggcgcttg ccgccgccgc atcgcttgat cccggcgtgt
     2221 acttcgccat gaacagccct gccgccctga tcggtacgga tgccaacacc gccgccgaag
     2281 tgattaccac caagctgcaa ttccctgtcg atgcggcaac cctgttgcat accgctaagg
     2341 aagtgggcga aaacaccatc ctttcccgtg caggcggtgc gccaaccctc gcagtcggta
     2401 tggcgcacat tatgagccgc ctgattccgg gcgaggcgat gatggcgttc tggtatcact
     2461 tcgccctatt gtttgaagcc ttgttcatcc tgaccgccgt cgatgccggt acgcgcgtcg
     2521 cacgttttat gattcaagac ttgggcagca tcttctacaa acctttcggc aacaccgact
     2581 ccatccccgc caacctgatt gcgaccttct tcgccgtggc attgtggggc tacttcctct
     2641 acaccggcgt aaccgacccg ctgggcggca tcaactcgct ctggcctttg ttcggcatcg
     2701 ccaaccaaat gctggcaggc gtggccttga ttatgtgcgc cgtggtgctg attaagatga
     2761 aacgcgaccg ttatgtttgg gtggtactcg ttcccgccgt cggcgtactg ttcgtaacct
     2821 gctacgccgg cctgcaaaaa ctgttccaca gcgacccgcg catcagcttc cttgcccacg
     2881 ccggcaaata cagcgacgca ttggctaaaa acgaagtcct tgcgcctgcc aaagacatcg
     2941 gcgaaatggc gcaaatcatc ttcaacgaca agattaatgc cggtctgacc atcctcttct
     3001 tgtcggttgt cgtgattgtc gccgcgtacg gtttgcgtac cgccctcaaa gcacgcaaag
     3061 tcggctggcc gaccgccaaa gaaatcccgg cggtgtaccg cgacggcaag cagccggagg
     3121 cacaaagtga agcataagct cgcgtcttgg tggaaaacca tcaagctgac ggcaaacttg
     3181 atggcaggcg tgcctgatta tgaaaactac gttgcacagc agcgcaaaca taatcccaac
     3241 gcccccgtga tgaccaagct gcaatttcaa gactactgcc gcaagcgccg ttgcggcgca
     3301 aacggcggac gctgctgtta agcctgcttg aaacaaattc cgtctgaacg ccgcttcaga
     3361 cggaattatt ata
//
LOCUS       DAWFBO010000175         3271 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFBO010000175 DAWFBO010000000
VERSION     DAWFBO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.175"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(63..2234)
                     /locus_tag="WGO87_001841"
     CDS             complement(63..2234)
                     /locus_tag="WGO87_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0393159.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNAKAVLDTITVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKEYEAGKGFRNFNGGKT
                     VPYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTVGDASSRTNITR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVVGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFEISGKKKDPKDPKKE
                     IDKTDEEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVK
                     THDGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSHSKPRFYDTHPKKLLSANPEFGAQVGRTWTASLAYR
                     FQNPNLEIGWRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDVFANWKPLGKDT
                     LNVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            2976..3122
                     /locus_tag="WGO87_001842"
     CDS             2976..3122
                     /locus_tag="WGO87_001842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393160.1"
                     /translation="MMRNGGNLSGITENERKSGQGGEAADSTDRTEPIHLVLEHLRES
                     FSLS"
BASE COUNT          726 a          758 c          923 g          864 t
ORIGIN      
        1 aaattatcag aacagatgcc gtctgaaagg ctttcagacg gcattttttc gggatgtgcg
       61 ttttagaact tgtagttcac gcccaagcgt acatcgcggc ccacgcccgg cagggtattg
      121 gtccagcgtt ggctgtgcgg atagtagaac gtgttgaaca cgttgttaac cgaaagatta
      181 acattgagcg tgtctttgcc cagcggtttc cagttggcga agacatcgtt cacaccgaaa
      241 cctttgcgta caacgttttc caatttgccg ttgcggtctt tttgacctgc caccaatatc
      301 gaacccacgg ctttttgaac ataacggccg cgccagccga tttccagatt cgggttttgg
      361 aagcggtagg caagggaagc cgtccaagtg cggccgactt gtgcgccaaa ttcaggattc
      421 gcgctcaaca gttttttagg atgggtatcg taaaagcgcg gtttgctgtg gcttacgccg
      481 actttggcag tcaggccgcc ggtgcggtag gacgcgccca attcgtaacc gtggtttttg
      541 atgtaaccgg cattgacggc ttcacggacg gcggcagaga cgtggcggtt ttgcggattg
      601 gcaagcgcgt ctttgatggt ctgccagaag tagctgccgt tggcggcaaa cgtgccgtcg
      661 ttgtagttga agccgatttc ggtattgcgc gcgcgttcgg ctttggtgcc gtcggcaatc
      721 gagatgatgc cgcgtttgcc gtgggtttgc agcgcgtcat acaggcgcgg gctgcggctg
      781 gcgtagttgt ggcttgcgct gaagctccag tgttcgtgcg gctgccaaat cacgccgaaa
      841 ctcgggttaa ggttgctgct tgaaacggtt ttgccgtcgt gggttttcac cttgaagcgg
      901 tcgtaacgca gcccgccggt cagggtaaag ccgtcaatct cgtgaatggc ttcgatatac
      961 gcgccggtat cggttttggt cgggttggac agtttgtagg aatggacaag atccatatct
     1021 ttcttgtctt tcgctttttc ttcatcggtc ttatctattt cttttttggg atctttcgga
     1081 tctttcttct taccggagat ctcaaattcg ccgttcaaaa acgcttgcgg tttgatttcc
     1141 tgatggcggt agttgatacc gtatttcaac agggtttgtt cggcaaggcg gctgtcaaag
     1201 ttgaagttca taccccgagt ggtgattcgg gtgtggttag ggccgaccac attgcctgcg
     1261 tagccgttat ctttgtcatc ggcggaatag cgtttctttt ccaacacata ggcgttggca
     1321 tccagttttt cgacaaagcc caaatctttg ccggtgtacg ccaagttggt gttggattgt
     1381 gtggtttcgc ggtaggatgg ggcttggcgt gtaatatttg tccgtgaact tgcgtcgcca
     1441 acggtaaatt cttcacggac ggtacggatg cctcggtgct ggtctttcat gtggctcaat
     1501 acgatgcggt ggtcgccgtc gccgaagctt gttccgattt tggccaggta gctgcgtttg
     1561 tccagtgcgc tgtacggtac ggttttgccg ccgttgaaat tacggaagcc tttgccggct
     1621 tcatattctt tttcatcgtt gcggttgtaa gagaacaaac cgtcgaagtt gccctctttt
     1681 ccgaatacgc tggcgccgta gcttacgcct tcgttgctgg caaagccgct gttgaggcgc
     1741 acgccccagt ttttatccaa gcctttgagc aggtcttggg catcgacggt tttggcgata
     1801 atcgcgccgt tggttgcgcc gataccggca gaggcagaac ccgcgccttt ttgtacggaa
     1861 acgactttaa ccaaagcggg atcgacaata aatctgcctt ggtggtaaag gatttggctg
     1921 tcggaatagg cgttgtccac cttgatgtcg acagagttct gacccatgcc gcgcagcgtc
     1981 aggaattggg acgtgccgtt gccgccgccg aaatcgatgg acggctcttc ttttaagagt
     2041 tcgcgcatat cggttgcggt gctttcgtct ttttgttgaa gcgtaacgat gttggtacgg
     2101 attttgctgc cttggcggtc gccttttacg gtaatggtat ccagtacggc cttggcatta
     2161 ttttctgccg cgtgggcaaa acctgccgcc agggtaagcg agagcaggct gaggcggaaa
     2221 aacggggcgt tcatttgttc gtccttttga gtgtatgaag ggaagtaaat ccaaaccgtt
     2281 aaggtttggc aggataagaa aaataataat tatttttgtt tatattaacg agagggggga
     2341 tttgtgtaaa gctgattatc gtttttattt gcgaagtgtt gttttttgtt gacaggtttt
     2401 gtcggaaatg tatagtggac taacaaaaat caggataagg cgacgaagcc gcagacagta
     2461 cagatagtac ggcaaggcga ggcaacgctg tactggtttt tgttagtcca ctataaaaaa
     2521 cggcggaaat atagtggatt aacaaatgcg ggaatgacga aaagcaacct ttccgcgtcc
     2581 ttcccgcgaa agcgggaatc tagaacccct aacgcggcag gaatctatcg gaaaaaaccg
     2641 aaaccgaacg aacctagatt cccgcctgcg cgggaatgac gaggcctgcg cgggaatgac
     2701 gaaaagcaac ctttccgcgt ccttcccgcg aaagcgggaa tctagaaccc ctaacgggca
     2761 ggaatctatc ggaaaaaacc gaaaccgaac agactggatt cccgcctgcg cgggaatgac
     2821 gaagcctgcg cgggaatgac gaaaagcaac ctttccgcgt ccttcccgcg aaagcgggaa
     2881 tctaggaact caaagctgca agaatttatc agaaacaact ggaactcaaa aaaactagat
     2941 tcccgcctgc gcgggaatga cgaagggtta aagaaatgat gagaaacggc ggtaatttat
     3001 cgggaataac cgaaaacgaa cggaaatcag gacaaggcgg cgaagccgca gacagtacgg
     3061 ataggacgga accgattcac ttggtgcttg agcaccttag agaatcgttc tctttgagct
     3121 aaggcgaggc aacgacgtac tggtttttgt tcatccacta taacagcaac cctgtcgccg
     3181 tcattcccgc gaaagcggga atccagtccg ttcagtttcg gtcatttccg ataaattcct
     3241 gctgcttttc atttctagat tcccactttt g
//
LOCUS       DAWFBO010000176         3179 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFBO010000176 DAWFBO010000000
VERSION     DAWFBO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.176"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(237..1094)
                     /locus_tag="WGO87_001843"
     CDS             complement(237..1094)
                     /locus_tag="WGO87_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0393161.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADSAPLTDYLARRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGDTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(1156..1971)
                     /locus_tag="WGO87_001844"
     CDS             complement(1156..1971)
                     /locus_tag="WGO87_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0393162.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            2035..2205
                     /locus_tag="WGO87_001845"
     CDS             2035..2205
                     /locus_tag="WGO87_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393163.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            2215..3000
                     /locus_tag="WGO87_001846"
     CDS             2215..3000
                     /locus_tag="WGO87_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0393164.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLSKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDAWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT          708 a          783 c          905 g          783 t
ORIGIN      
        1 gcacttgaaa tccgaatcca agttattctt cattgtattc tacctggggt cgtagttatt
       61 aaattaccat acttatttgt ttggatcgtc ataatattag ttggcatgtt gttgttatat
      121 ttatcatatc caatatcctt attaactcgg atgactcttt cataaattgt atgggaaatg
      181 agatttccat cactaagcct ctcaatagat atacgcgtta atgttgggaa agggtttcag
      241 acggccttca taatgcgttc gatttcgtgc caaaagccgt ccggccgaca ttccaaaacg
      301 gcaatcaagc cttggtcgat acggcggatt tcggttgcgt tcaatgtttc ggtttcggcg
      361 acacgcgcag gcgcgaggta ggccatacgg tcgagcagat ggtagcggac ttcttggcgt
      421 ttaaacccgt attccgccca atcttgaata tagatgccgc gccaaccgta tggattaagc
      481 ggtggctcaa aagcatggaa accgtgtgga aaaaatccga tgccgcgcac gatggaaacg
      541 ggttttacgt caagcggcag accgtgttgc cgcaaggttc ggatgccgtc tgaagtgtgc
      601 ggcagaccga gttgggcggt cagtttggcg gcttttccca acagcgtgtc tttggggtcg
      661 agtccgcgca tgtcttcgag cctgcccgtg tcgccgccgt aatatttgca ggtcagctcg
      721 atatggtagg gcttgccgtt aagccttacc acaaaatccg ccgcgccttg cgtattgccg
      781 tccgaaccgg aaaccgtgag gttgtgcgcg agcagttcgg cgtgtggcgc gttggcaaac
      841 caaaatgcca gcagttcttc ggcgtaaatg cccaagcggt gtccgaacgg ggcgcgtcgg
      901 gcgaggtaat ccgtcagcgg agcggaatcg gcatccaatg ccaaaagata gcggaaaccg
      961 cgttccccca atagttcgcg cacgcccaat tcgcaaccgc tttgccacaa aggcggcgca
     1021 gtcagcagcg aggcgaggtc gcggacgggt tggctggtga gcttccacca taatgcgtct
     1081 agggcgtaat tcatgcttat ccgaatatca gtcatgccgt ctgaaaagct ggaaatgttt
     1141 tcagacggcc ttaaatcagt cttttttatg gatgcgatgg ctgacggtat tgaaaaaacc
     1201 gcgcaggatg tcgatgtctt cggtttgcgt gttggcgcgt ccgaacaggc tctgcatacg
     1261 gcgcatcaga cgctcgccgt tgcggcggtt gaaaaagccg atgtcgttca tcacgctttc
     1321 catgtgggcg accatgcctt tgatttgctc gtgggtcgca gcgtggtctt cttgttgaag
     1381 atgggtcatg ggcgaaccgg tttggctgaa gatttcgtag cacacgacct gcacggcttg
     1441 ggcgaggttg agcgagaaat agtcgggatt gccgttgatg gtcatcagtc ggttgcaggc
     1501 ttggacttct tcgatgctca agccgaaagt ctcgttgccg aaaaccagtg ccactttctc
     1561 gcctcggttt gcggtctgca gtaattcgga taccaaatcg cgcggggttt gcagcggcgc
     1621 agtaatttcg cggcggcggc tggtcagggc gcaggcgatg gtggtgtcgg caagggcttc
     1681 gtccaaagaa gcggcaatgg tggcattttc caaaacgtct gccgcgccgg aagcgaggat
     1741 gaagctttct tccggtaatt taaacgattg aggatgctcc gggtcaaaca cgggcgggtt
     1801 ttccgtcatc ggcgttgcca tcagatttgg ggcgacgatg gtcagtttgt gcagacccat
     1861 tgttttcatc gcgcgcgcgg cagagccgat gttggcggga tggctggtgc gcgtgaggat
     1921 gatgcggatg ttgtccagat aagcgggcag ggcgggtttg agagtagtca tgttcggttt
     1981 tcggtcggaa tggaataggg tataatgcgc cgtctttttc gttcggacgg taaaatgccg
     2041 tctgaaatgt tctttaacaa cgtcggaaat ttaacgttgc gcctgaagca gacacgcgtc
     2101 gggcggtatt ttacccgatt tggcaggatt taccccaacc aaataaactc cgattttgcc
     2161 gctccgaagg actgcgcgtc cgaaccggcg gtatgccacc cttaaggaaa tctgatgaat
     2221 ccgtttttga atacagcctt taaagccgcc cgccgtgccg gtcagatgat gatacgcgcc
     2281 gcaggtaacc tcgatgccgt caaaaccgac agcaaagcct tcaacgattt tgtttccgat
     2341 gttgaccgca attccgaaat catcctggtt gaggctttga aagaagccta tccgcaccac
     2401 aaaatcactt gtgaagaaag cggctcccac ggcaaagccg cagccgagta cgaatggatt
     2461 atcgatccgc tcgacggcac gaccaatttc cttcacggtc atccccaata cgccatctct
     2521 atggcgctcc tgcacaaagg cgtgttgcaa gaagctttgg tttacgcccc cgaacgcaac
     2581 gacgtatata tggcttcgcg cggcaaaggc gcgttgctca acgaccgccg catccgcgtt
     2641 tccaaccgca tcgaattgaa ccgctgcctg atcggtaccg gcttccctgt tgtcgatcaa
     2701 agcatgatgg acaagtatct ggcgattttg aaagatttct tgtcaaaaac cgccggcggc
     2761 cgtcgtgaag gcgcggcttc tttggatttg tgtgctgtag caacaggtcg ttttgacggc
     2821 tttttcgagt ttaacctcaa accgtgggac attgccgcag gtgcattgat tgttcaggaa
     2881 gcgggcggta tcgtaaccga tatgacgggt gaggacgctt ggttggaaag cggcgatatt
     2941 gtcgccgcca atcccaaagt attggcgcag atgctgaaaa tcatttccgc acacgtttaa
     3001 atttcagccc tttcccgaaa atatgggaaa acgggcgggt atgccgtctg aagtatcgcg
     3061 ccgatatttc agacggcatt tttattggca acgcggcagg acggcggaaa cggtcaaatg
     3121 ccccaaactg ccgaggatgg aaacccatat ctgattaaac ggagaaccct gtgcgattt
//
LOCUS       DAWFBO010000177         3170 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFBO010000177 DAWFBO010000000
VERSION     DAWFBO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.177"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(57..1157)
                     /locus_tag="WGO87_001847"
     CDS             complement(57..1157)
                     /locus_tag="WGO87_001847"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0393165.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPGR
                     LRRKLLDLQDTGADELHRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHSYSVQLADLPLLAERTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGEKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAKCFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1482..3056
                     /locus_tag="WGO87_001848"
     CDS             1482..3056
                     /locus_tag="WGO87_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0393166.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          655 a          795 c          924 g          796 t
ORIGIN      
        1 aaagaggaaa aaatgccgtc tgagcggcgg aacgggcttt cagacggcat ttgcgctcaa
       61 taataatatc ccgcgcccag aatacacggt ttggatgcgc cggttgcttt gtgcggacta
      121 ccggggatgc gattaatcca acacgccgcc aaccacgcaa atgcggcggc ttctacccat
      181 tgcggatcga ggttcagttc ggcggtgctg tgcagggaaa cgcgtgtgcc gaagcatttt
      241 gccaaatccg ccattaaaac aggattgcgg atgccgccgc cgcaaatgta catttgacgg
      301 gcatctgccg ctgcgtgtga gacggcgtcg aaaacggttt gcgcggtaaa acgggaaagc
      361 gtccgtaata cgtcgtatcg gttttcgccg ccgtcaaggt aggtttcgag ccaatttagg
      421 gcaaacagtt cgcgccccgt gcttttgggg tggggttgtg cgaaatatgg gtgggcgagc
      481 agcctgtcga gcagttgcgg caatatgttg ccttgtgccg ctttttcacc gtttttgtcg
      541 taaggaagct gccagtgtgc ctgcgtccac gcgtccatca gcatattgcc cggccctgtg
      601 tcgaagccga aggcgggtgc gtcggggggg agtacgctga tgttggcaat cccgccgatg
      661 ttcagtacca cgcgtgtttc tttgtcatcg cggaacaggg ctttgtgaaa ggcggggacg
      721 agcggcgcac cttgtccgcc ggccgcaagg tcgcggctgc ggaagtcgcc gacggtaaaa
      781 atctgagtcc gttccgccag cagcggcaaa tcggcaagct gtacgctgta actgtgttcc
      841 ggcgcgtgtc tgacggtttg cccgtggcag ccgagggcgg taatgtcgga cggcgcgagg
      901 ttttgactgc acagcagttc ggcggcggtt tgcgcgtaca ggcggctgag ttcttgcgac
      961 aacatcctgc tgcggtgcag ttcgtccgcg cctgtgtcct gcaaatccag caatttgcgg
     1021 cgtaacctgc cggggtaggg ggtaaaggcg tgcccttccg cgcccagcca tttgccgccg
     1081 tccatccgta ccagcacggc atccgcccca tccatactgg ttcccgacat gatgccgatg
     1141 taaagctgtg tttccatcat cactcccaaa ctggtgcaaa acgccatttt aacgtgtatt
     1201 gacgctcgta tactgatttg ccgccgcagt gtaaataaag tgtaaataaa tgtttcaaga
     1261 cggatggaaa aatattataa tgcgcccgca acatccagta gtagaagtgt catacaaacc
     1321 gtttccggca gcagttttgc attcggtcag gtttgggggt attcggatgc ggttcagaag
     1381 gaaggatgcg tctgccatat cccgaaacgg cagttcgacc ggaggcagca gtacagtgtc
     1441 ggcaacactc atgatttcca ccacattaaa ggaagattgc catggctcaa atccaaatga
     1501 gcgcaaatgt taaaaccatc aacgccgtct ttgccgccat gctggtaggt acagtcggct
     1561 attttattta ttggggcttg ggttataccc attacaatta cgccgcctta ttcattattg
     1621 ccacgatgtt cggcgtgttt atggcgttca acatcggcgg caacgatgtt gccaattctt
     1681 tcggcaccag cgtcggtgcg ggtacgctga ccatcccgca ggctttgctg attgcggcgg
     1741 tatttgaggt cagcggcgcg gtcatcgcgg gcggtgaggt aaccaatacc atacgcaaag
     1801 gcatcgtcga tttgaagggt gttgatttcg aacccataca gtttgtgttt attatgatgt
     1861 ccgcgctttt ggcggcggcg ttgtggctgt tgtttgcctc gaaaaaaggg cttccggtat
     1921 ctaccaccca ttccattatc ggcggcattg tcggcagcgc ggtatgtatg gcggtaatga
     1981 acgatgccgc atcgggcgat ttgatacgtt ggggcaagct gggcggtatt ggtgtttctt
     2041 gggtattgtc gcccgtgttg ggcggcgcgg tgtcctattt cctgttttcg cgcgtcaaga
     2101 aaaacgtctt agattacaac gcttgggcgg aaggcacgct caagggcatc aagcaggaaa
     2161 aaaaggccta taaagaacgg caccgcctgt ttttcgaggg tttgtccgaa gccgaaaaag
     2221 tcgagtacgc caccaaaatg gcgcacgacg cgcaaattta cgacgaaccc gaattcgatc
     2281 cgcaagagct gcaatcggag tattaccgcg gtctttatgc gttcgacaac cgtaaaaaca
     2341 atgtcgattc ctacaaagcg ctgcattctt ggattccctt tatcgcttcg ttcggcgcga
     2401 tgatgatttc cgctatgctg attttcaagg gcttgaaaaa cctgcatttg gggatgagca
     2461 acgtcaacag cttcctgacc atctttatga taggcgcggc ggtgtggatg gggacgtttg
     2521 tgtttgccaa aagcctcaag cgtaaagact tgggcaaatc gacctttcag atgttttcat
     2581 ggatgcaggt ctttaccgcc tgcggcttcg cattcagcca cggtgcgaac gatatcgcca
     2641 acgccatcgg tccgtttgcc gcgattatgg atgttttgcg taccaacagc gttgccgcgc
     2701 aaaatgtcgt ccccccgatt gcgatgctga ctttcggtat cgcgctgatt gtcggtttgt
     2761 ggtttgtcgg taaagaggtg attaaaaccg tcggtacgag tttggcggaa atgcatcctg
     2821 cttcgggttt taccgccgaa ctgtccgccg catccgtcgt gatgggcgcg tcgctgatgg
     2881 ggctgcccgt gtccagtacg catatcttgg tcggcgcggt actcggcatc ggtctggtca
     2941 accgcaatgc caactggaaa ctgatgaagc ccatcggttt ggcgtgggtc attaccctgc
     3001 ctgccgccgc cgtattgtcg gttgtctgct acttggtttt acaggcagta ttctgattgt
     3061 aaaatactga tgccgtctga acccgtgttc agacggcatt ttgttgatgg aatgtgcggg
     3121 cttgtgcctt atgcacaatc tgttctgtcg ggatatgccg tttggtatag
//
LOCUS       DAWFBO010000178         3148 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFBO010000178 DAWFBO010000000
VERSION     DAWFBO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.178"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            98..796
                     /locus_tag="WGO87_001849"
     CDS             98..796
                     /locus_tag="WGO87_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0393167.1"
                     /translation="MVRTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNRIHPFYSDSKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPTPLKAYNLTVADWHTYFVKSSQAETEGVWVHNDCPPYKRPNNATTKAQRESVQGKP
                     CVEYGKLAEKMIADHKKPLVVEYYETGTIDKSKMRAIESVQPQCPTCSAKQGGRLSQY
                     SKEQKRKLSNGNKK"
     gene            780..1133
                     /locus_tag="WGO87_001850"
     CDS             780..1133
                     /locus_tag="WGO87_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393168.1"
                     /translation="MGIKNEQIIICKKYNTEIYPISDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1123..1446
                     /locus_tag="WGO87_001851"
     CDS             1123..1446
                     /locus_tag="WGO87_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0393169.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1505..1732
                     /locus_tag="WGO87_001852"
     CDS             1505..1732
                     /locus_tag="WGO87_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393170.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1860..2402
                     /locus_tag="WGO87_001853"
     CDS             1860..2402
                     /locus_tag="WGO87_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0393171.1"
                     /translation="MINHIEKYTGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKHDLNYKSRSEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVGNLSDMNRKPFTW"
     gene            2483..3019
                     /locus_tag="WGO87_001854"
     CDS             2483..3019
                     /locus_tag="WGO87_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0393172.1"
                     /translation="MDYNQTVLSHLQNFWKHHDIKGFTWALGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDRFQLGKTVN
                     IGRPWVEQSSFQHFLISLPYPYGQELEYMDNVRFFWLLPITQPERLFLNTHSVEDLET
                     RFDELGIDYLDINRASVI"
BASE COUNT         1016 a          578 c          636 g          918 t
ORIGIN      
        1 gtgtttaatt ttcaggcgac cttcccctta tttgaaacag gatattggga actaagttct
       61 tcaaaaatcc tacacctgct ccttccacgg cagcaccttg gtcagaacgg cagacggcta
      121 caaagccatt gcccatattc aagccggcga ccgcgtcttc gccaaagatg aaaccagtgg
      181 aaaaactgga tacaaacctg ttaccgcccg atacggcaat ccgtaccagg aaaccgttta
      241 cattgaaatt tcagacggca tcggcaacaa ccaaaccctg atttccaacc gcatccatcc
      301 gttttattcg gacagcaaat ggattaaagc ggaagatttg aaagcgggaa gcaggctgtt
      361 atccgaaagc ggcaaaaccc aaaccgtccg caacatcgtt gtcaaaccaa caccgctcaa
      421 agcctacaat ctgaccgttg ccgactggca tacctacttc gtcaaaagca gtcaggcgga
      481 aacggaaggg gtttgggttc ataatgattg tccgccctat aagagaccaa ataacgcaac
      541 gacaaaagca caacgagaaa gtgtacaagg aaaaccctgc gttgaatacg gtaaattagc
      601 cgaaaaaatg attgccgacc ataaaaaacc attagttgtc gagtattatg aaacagggac
      661 aattgacaaa tctaagatga gagcaattga atcagtacaa ccgcaatgtc cgacttgttc
      721 tgcaaaacaa ggggggagat taagccaata ttcaaaagaa cagaaaagga aactgagtaa
      781 tgggaataaa aaatgaacaa ataataattt gtaaaaaata taatacagaa atatatccta
      841 tttccgatgt ttcaaaaatc ggtgttgctg aaaatgtcaa acaggcaaac ctgtatccga
      901 ttaacgggtt aaggcaccgt cccaaaggtg atactaacgg ttggtatatc tgggccggtg
      961 aaaacttctc ctatgataaa aattttttcc tgcccttgca tactttccat ttgcaaatat
     1021 ggcgacccga aatcatcccc tttttgacac ttccgcccgg ttaccgtttt ttaattgggg
     1081 aaaacggtta tgaagacgta tggtttgatg agttgctatt aaatgataat tgacgaaaaa
     1141 tctacttcag atgtactgaa acaatatcaa gaactagggt ttgatttgac aaaacctatg
     1201 ataatagagt tttttatagg aggttctcaa aagaatctgc aatcaataga aagccggctc
     1261 atttcttatc ggcaagattt tcaaatcagt attgaacaag atgaattcgg agaaatgtgg
     1321 acttgctatt gttccgtcaa tatcgtccct tcccttgaaa acattttagc tattgaaact
     1381 acgttatttg gtattgcaca aaaaaatgat tgtaattatg aaggttttgg ttcgtatggg
     1441 aactaacaaa aagtgtaggt tgggttgaaa aaaccatatt cccattaaag attagggtgt
     1501 gtctatggac ataaaattca atactttggg ggttatcctg aatggggtca atcctgaaga
     1561 aaaattcata aaaattattg atgatcagga aaataccggg ggatttctga tactgttatc
     1621 ttcaaatgac aaattctcat catttgacag ttacgatgac tgggtcgaaa acttagaaat
     1681 attaaaagaa tacctgcaag aatctcattg gataataaag tgggtcggat agtcggcaaa
     1741 cgtagtccgt ctgaaacgct tttacttttc agacggactt tctatcaatg ggaacaaggc
     1801 gttttgtctc aatctggtct atgattattt cgatacagtg atgggaagct aagaaaaata
     1861 tgattaatca tattgaaaaa tatacaggca ctatttctca tggctcgaaa agtgattcgg
     1921 gaagtcagta caaattaaat atcgcagcta ttccctcttc ccctaacagg gatttaaaaa
     1981 cttatattac tttagggtta agcaaacatg atctgaatta taaaagcaga tctgaaatac
     2041 tttttgtatg ttctttaaaa tatgatgaaa atcagatttt tccattttta agatggttgg
     2101 cagaaaccat cattgagaat aaaaaaatcc ttcttcgggg gcaggttgtt tatttgccca
     2161 gaagcattgt tgattcaaca aaaatggatg cactgtatgt ttccgcccca ttttatttcg
     2221 atgatgattt tcaggtctgt tatggcgaac actacaatat cgttttccct ttgcttgtcc
     2281 ccttgtataa acaggaagca gaattggtgg aaaagaaagg ttggaatgct tttgagcagt
     2341 tcttgttgga taatgaagtt ggcaaccttt cggatatgaa tcggaaaccg tttacttggt
     2401 aaataccttc ttcaaacggt caatgccgtc tgaaaaacct tgtttcaggt ttcaggcgac
     2461 ctttgaaaac aggctaaaaa gcatggacta taaccaaact gttttatctc atttgcaaaa
     2521 tttttggaaa catcatgata ttaaaggatt tacttgggct ttaggaagga ttgttgaaga
     2581 acttcccgat ttccaagttt tccaagtgat tcctaatcat gaagatgagc cttgggttta
     2641 tgtcagctct ggaataggtc aatttttagg gcaggagttt tttatcatca gcccatttga
     2701 aacacctgaa catattgaga ccttggcaat gctggcatct gcaagtatgc attatcccga
     2761 tcgatttcaa ttagggaaaa cggtcaatat cggcagaccg tgggttgagc aatcatcctt
     2821 ccagcatttt ctgatttctc tgccttatcc ttatgggcaa gaattggaat atatggataa
     2881 tgtccgattt ttctggttat tgccaattac ccagccagaa agacttttct tgaatactca
     2941 ttcagtagaa gacttggaaa cgagatttga tgagctaggt attgattatt tggatataaa
     3001 tcgtgcaagc gttatttgat atttatgttg tttgaaatct ttcagcaagc gtaggttgga
     3061 ttggaaatcc aacaatcaaa atgccgtctg aaaccgtgtt taattttcag acggcatttc
     3121 cttcatttga aataggatat tgagaact
//
LOCUS       DAWFBO010000179         3091 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFBO010000179 DAWFBO010000000
VERSION     DAWFBO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.179"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            7..2898
                     /locus_tag="WGO87_001855"
     rRNA            7..2898
                     /locus_tag="WGO87_001855"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2995..3087
                     /gene="rrf"
                     /locus_tag="WGO87_001856"
     rRNA            2995..3087
                     /gene="rrf"
                     /locus_tag="WGO87_001856"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          848 a          660 c          924 g          659 t
ORIGIN      
        1 tgatagagtc aagtgaataa gtgcatcagg tggatgcctt ggcgatgata ggcgacgaag
       61 gacgtgtaag cctgcgaaaa gcgcggggga gctggcaata aagctatgat cccgcgatgt
      121 ccgaatgggg aaacccactg cattctgtgc agtatcctaa gttgaataca tagacttaga
      181 gaagcgaacc cggagaactg aaccatctaa gtacccggag gaaaagaaat caaccgagat
      241 tccgcaagta gtggcgagcg aacgcggagg agcctgtacg tgataactgt cgagatagaa
      301 gaacaagctg ggaagcttga ccatagtggg tgatagtccc gtattcgaaa tctcaatagt
      361 ggtactaagc gtacgaaaag tagggcggga cacgtgaaat cctgtctgaa tatgggggga
      421 ccatcctcca aggctaaata ctcatcatcg accgatagtg aaccagtacc gtgagggaaa
      481 ggcgaaaaga accccgggag gggagtgaaa tagaacctga aacctgatgc atacaaacag
      541 tggaagcacc ctagaggtgt gactgcgtac cttttgtata atgggtcaac gacttacatt
      601 cagtagcgag cttaaccgaa taggggaggc gtagggaaac cgagtcttaa tagggcgatg
      661 agttgctggg tgtagacccg aaaccgagtg atctatccat ggccaggttg aaggtgccgt
      721 aacaggtact ggaggaccga acccacgcat gttgcaaaat gcggggatga gctgtggata
      781 ggggtgaaag gctaaacaaa ctcggagata gctggttctc cccgaaaact atttaggtag
      841 tgcctcgagc aagacactga tgggggtaaa gcactgttat ggctaggggg ttattgcaac
      901 ttaccaaccc atggcaaact cagaatacca tcaagtggtt cctcgggaga cagacagcgg
      961 gtgctaacgt ccgttgtcaa gagggaaaca acccagaccg ccagctaagg tccccaatga
     1021 tagattaagt ggtaaacgaa gtgggaaggc ccagacagcc aggatgttgg cttagaagca
     1081 gccatcattt aaagaaagcg taatagctca ctggtcgagt cgtcctgcgc ggaagatgta
     1141 acggggctca aatctataac cgaagctgcg gatgccggtt taccggcatg gtaggggagc
     1201 gttctgtagg ccgatgaagg tgtattgtaa agtgtgctgg aggtatcaga agtgcgaatg
     1261 ttgacatgag tagcgataaa gcgggtgaaa agcccgctcg ccgaaagccc aaggtttcct
     1321 gcgcaacgtt catcggcgta gggtgagtcg gcccctaagg cgaggcagaa atgcgtagtc
     1381 gatgggaaac aggttaatat tcctgtactt gattcaaatg cgatgtgggg acggagaagg
     1441 ttaggttggc aagctgttgg aatagcttgt ttaagccggt aggtggaaga cttaggcaaa
     1501 tccgggtctt cttaacaccg agaagtgacg acgagtgtct acggacacga agtaaccgat
     1561 accacgcttc caggaaaagc cactaagctt cagtttgaat cgaaccgtac cgcaaaccga
     1621 cacaggtggg caggatgaga attctaaggc gcttgagaga actcaggaga aggaactcgg
     1681 caaattgata ccgtaacttc gggagaaggt atgccctcta aggttaagga cttgctccgt
     1741 aagcctagga gggtcgcaga gaataggtgg ctgcgactgt ttattaaaaa cacagcactc
     1801 tgctaacacg aaagtggacg tatagggtgt gacgcctgcc cggtgctgga aggttaattg
     1861 aagatgtgag agcatcggat cgaagcccca gtaaacggcg gccgtaacta taacggtcct
     1921 aaggtagcga aattccttgt cgggtaagtt ccgacccgca cgaatggcgt aacgatggcc
     1981 acactgtctc ctcctgagac tcagcgaagt tgaagtggtt gtgaagatgc aatctacccg
     2041 ctgctagacg gaaagacccc gtgaaccttt actgtagctt tgcattggac tttgaagtca
     2101 cttgtgtagg ataggtggga ggcttagaag cagagacgcc agtctctgcg gagccgtcct
     2161 tgaaatacca ccctggtgtc tttgaggttc taacccagac ccgtcatccg ggtcggggac
     2221 cgtgcatggt aggcagtttg actggggcgg tctcctccca aagcgtaacg gaggagttcg
     2281 aaggttacct aggtccggtc ggaaatcgga ctgatagtgc aatggcaaaa ggtagcttaa
     2341 ctgcgagacc gacaagtcga gcaggtgcga aagcaggaca tagtgatccg gtggttctgt
     2401 atggaagggc catcgctcaa cggataaaag gtactccggg gataacaggc tgattccgcc
     2461 caagagttca tatcgacggc ggagtttggc acctcgatgt cggctcatca catcctgggg
     2521 ctgtagtcgg tcccaagggt atggctgttc gccatttaaa gtggtacgtg agctgggttt
     2581 aaaacgtcgt gagacagttt ggtccctatc tgcagtgggc gttggaagtt tgacgggggc
     2641 tgctcctagt acgagaggac cggagtggac gaacctctgg tgtaccggtt gtaacgccag
     2701 ttgcatagcc gggtagctaa gttcggaaga gataagcgct gaaagcatct aagcgcgaaa
     2761 ctcgcctgaa gatgagactt cccttgcggt ttaaccgcac taaagagtcg ttcgagacca
     2821 ggacgttgat aggtggggtg tggaagcgcg gtaacgcgtg aagctaaccc atactaattg
     2881 ctcgtgaggc ttgactctat catttgaaga acttcaagag ataaaagctt actgactgat
     2941 tcagtcatta ccgaatatat tgattaaggc tttaccgatt tgtaacagtt taagtttggc
     3001 ggccatagcg agttggtccc acgccttccc atcccgaaca ggaccgtgaa acgactcagc
     3061 gccgatgata gtgtggttct tccatgcgaa a
//
LOCUS       DAWFBO010000180         3087 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFBO010000180 DAWFBO010000000
VERSION     DAWFBO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.180"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            110..2878
                     /locus_tag="WGO87_001857"
     CDS             110..2878
                     /locus_tag="WGO87_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0393173.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGGLKFNSNSGKWER
                     DFQKSYWKTKWYQKYDDPQELQKYIEGHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYREHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
     gene            2908..>3087
                     /locus_tag="WGO87_001858"
     CDS             2908..>3087
                     /locus_tag="WGO87_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393174.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVVYES"
BASE COUNT          863 a          769 c          755 g          700 t
ORIGIN      
        1 attgaaatct tcagacggta tatcgaattt acacttttta atgtttatgc cgcctaaaaa
       61 aatgctaata tatttcttaa ttgtctgact gtttattgtt gaggaaaata tgagatcttc
      121 tttccggttg aagccgattt gtttttatct tatgggtgtt atgctatatc atcatagtta
      181 tgccgaagat gcagggcgcg cgggcagcga ggcgcagata caggttttgg aagatgtgca
      241 cgtcaaggcg aagcgcgtac cgaaagacaa aaaagtgttt accgatgcgc gtgccgtatc
      301 gacccgtcag gatatattca aatccagcga aaacctcgac aacatcgtac gcagcatccc
      361 cggtgcgttt acacagcaag ataaaagctc gggcattgtg tctttgaata ttcgcggcga
      421 cagcgggttc gggcgggtca atacgatggt ggacggcatc acgcagacct tttattcgac
      481 ttctaccgat gcgggcaggg caggcggttc atctcaattc ggtgcatctg tcgacagcaa
      541 ttttattgcc ggactggatg tcgtcaaagg cagcttcagc ggctcggcag gcatcaacag
      601 ccttgccggt tcggcgaatc tgcggacttt aggcgtggat gacgtcgttc agggcaataa
      661 tacctacggc ctgctgctaa aaggtctgac cggcaccaat tcaaccaaag gtaatgcgat
      721 ggcggcgata ggtgcgcgca aatggctgga aagcggagca tctgtcggtg tgctttacgg
      781 gcacagcagg cgcagcgtgg cgcaaaatta ccgcgtgggc ggcggcgggc agcacatcgg
      841 aaattttggc gcggaatatc tggaacggcg caagcagcga tattttgtac aagaaggcgg
      901 gttgaaattc aattccaaca gcggaaaatg ggagcgggat ttccaaaagt cgtactggaa
      961 aaccaagtgg tatcaaaaat acgatgaccc ccaagaactg caaaaataca tcgaaggtca
     1021 tgacaaaagc tggcgggaaa acctggcgcc gcaatacgac atcaccccca tcgatccgtc
     1081 cagcctgaag cagcagtcgg caggtaatct gtttaaattg gaatacgacg gcgtattcaa
     1141 taaatacacg gcgcaatttc gcgatttaaa caccaaaatc ggcagccgca aaatcatcaa
     1201 ccgcaattat caattcaatt acggtttgtc tttgaacccg tataccaacc tcaatctgac
     1261 cgcagcctac aattcgggca ggcagaaata tccgaaaggg tcgaagttta caggctgggg
     1321 gcttttaaag gattttgaaa cctacaacaa cgcaaaaatc ctcgacctca acaacaccgc
     1381 caccttccga ctgccccgtg aaaccgagtt gcaaaccact ttgggcttca attatttcca
     1441 caacgaatac ggcaaaaacc gctttcctga agaattgggg ctgtttttcg acggtccgga
     1501 tcaggacaac gggctttatt cctatttggg gcggtttaag ggcgataaag ggctgctgcc
     1561 ccaaaaatca accatcgtcc aaccggccgg cagccaatat ttcaacacgt tctacttcga
     1621 tgccgcgctc aaaaaagaca tttaccgctt aaactacagc accaataccg tcggctaccg
     1681 tttcggcggc gaatatacgg gctattacgg ctcggatgac gaatttaagc gggcattcgg
     1741 agaaaactcg ccgacataca gggaacattg caacccgagt tgcgggcttt atgaacccgt
     1801 attgaaaaaa tacggcaaaa agcgcgccaa caaccattcg gtcagcatta gtgcggactt
     1861 cggcgattat ttcatgccgt tcgccagcta ttcgcgcaca caccgtatgc ccaacatcca
     1921 agaaatgtat ttttcccaaa tcggcgactc cggcgttcac accgccttaa aaccagagcg
     1981 cgcaaacact tggcaatttg gcttcaatac ctataaaaaa ggactgttaa aacaagatga
     2041 tacattagga ttaaaactgg tcggctaccg cagccgcatc gacaactaca tccacaacgt
     2101 ttacgggaaa tggtgggatt tgaacgggga tattccgagc tgggtcagca gcaccgggct
     2161 tgcctacacc atccaacacc gcaatttcaa agacaaagtg cacaaacacg gttttgagtt
     2221 ggagctgaat tacgattatg ggcgtttttt caccaacctt tcttacgcct atcaaaaaag
     2281 cacgcaaccg accaacttca gcgatgcgag cgaatcgccc aacaatgcgt ccaaagaaga
     2341 ccaactcaaa caaggttatg ggttgagcag ggtttccgcc ctgccgcgag attacggacg
     2401 tttggaagtc ggtacgcgct ggttgggcaa caaactgact ttgggcggcg cgatgcgcta
     2461 tttcggcaag agcatccgcg cgacggcaga agaacgctat atcgacggca ccaacggggg
     2521 aaataccagc aatgtccggc aactgggcaa gcgttccatc aaacaaaccg aaactcttgc
     2581 ccgccagcct ttgatttttg atttttacgc cgcttacgag ccgaagaaaa accttatttt
     2641 ccgcgccgaa gtcaaaaatc tgttcgacag gcgttatatc gatccgctcg atgcgggcaa
     2701 tgatgcggca acgcagcgtt attacagctc gttcgacccg aaagacaagg acgaagacgt
     2761 aacgtgtaat gctgataaaa cgttgtgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgatgacg atgagctaca agttttaaag
     2881 gcagcccgca ttttgtagaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaatc cgttttatcg catcggcaac
     3001 tcaaagaaaa atccatttca ttcccacgca gggaagccgg tttttgattt cggttatttt
     3061 tggttgtttc gggtagttta tgagtcg
//
LOCUS       DAWFBO010000181         3080 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFBO010000181 DAWFBO010000000
VERSION     DAWFBO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.181"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(92..943)
                     /locus_tag="WGO87_001859"
     CDS             complement(92..943)
                     /locus_tag="WGO87_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ0393175.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1286..2800
                     /gene="katA"
                     /locus_tag="WGO87_001860"
     CDS             1286..2800
                     /gene="katA"
                     /locus_tag="WGO87_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0393176.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
BASE COUNT          743 a          870 c          794 g          673 t
ORIGIN      
        1 gatgcaggtt tccgcgcgga cggattcggc attcccgctt tcgcagaaat gccggctttt
       61 ggtttgttgt ctttataaga ttattcggca attattctgc ggttttgcgt ttgtgtgctg
      121 ccggaatctc aaaggattct ctgtatttgg caatggtgcg gcgagaaacc tccatgccgc
      181 ggaaagccag caggttggcg agcgcctcgt cagaatacgg cgtgggtgtg gacgaggcaa
      241 aaccagcggc gggaaaggga ttgagcgaag caatgaggtc gagtgcggct tcgagtgtgc
      301 cgctgtcggt ttggggcagg cgttttttta ttcgcgcgag ggtttggctg cggttgccgt
      361 caatgctgtc gagggcgttt cggacgatat gcagggcgga gggtttggca gcacattcgc
      421 ccgatctttc tatctgcagt atcagcgatt cgttcaaatc ggcggcggcc acgcctgccg
      481 ggtcgaattt tttcaatgcg gtcagggctt gtttcagcat tgcttcatcc aacatccact
      541 ccaagggcgt atggtcgagg atgtcttcga tgctgtcggt cagataacct tgctcgtcaa
      601 ggaaatcgat gaggatgtgg acacaggcgg attcttgatc ggaaagcggg tgttcgcata
      661 cttgcgcgtg caggtattgc ttgaaatcct gctcgccggc gatgttggac agcatatctt
      721 cgccttcgtc tccgccgatt tgacgggcag gcgcagtgta atggctgaac tcggcatcgg
      781 aaaattcatc cgtatctttg cgttcgagca gggggttgtc cgacagccag ttttcgacct
      841 cgcgttcaag ttcgataccc gacatctgca atacgcgcaa agattgttgc agccgctggt
      901 tgagctgctg ggtctgtttg agctttattc cgagtaacgt catgataatg cgaaagaatt
      961 attattttgg cctgtcggtg gcagatgatg ccgggaaaca ttattataaa tggttttatg
     1021 tagggatttt gattcgtgcc gataacaaaa aacaaacgaa aatcaaaaac cgattgctta
     1081 taacaatatt aaatcgattt catagtttta atagcgaaaa tcttagcgta tagtcgcatc
     1141 catagttttt acaaaaggaa aacaaaatgt cgattcaaga aatttattgc aatcaagaaa
     1201 ccggttacga atacgctttc cgccaaatcg tactgtaagc cgtttgtcgt atcttttgat
     1261 taaccaagga aaaggaacac gagctatgac tacctccaaa tgccccgtaa cccatctgac
     1321 catgaacaac ggcgcgcctg ttgccgacaa tcaaaacagc ctgaccgccg gtccgcgcgg
     1381 ccctctgctg gcgcaggatt tgtggctgaa tgaaaaactc gccgacttcg tgcgcgaagt
     1441 catccccgaa cgccgtatgc acgccaaagg ttcgggcgcg ttcggtacgt ttaccgtaac
     1501 gcacgacatc accaaataca cccgcgccaa aatcttcagc gaagtcggca aaaaaaccga
     1561 gatgttcgcc cgtttcacca ccgtggcagg cgaacgcggc gcagccgatg cagaacgcga
     1621 catccgcggt tttgccttga aattttatac cgaagaaggc aactgggatg tggtcggcaa
     1681 caacacgccc gtgttcttcc tgcgcgaccc gcgtaagttc cccgacctga acaaagccgt
     1741 caaacgcgac ccgcgcacca atatgcgctc tgccacaaac aactgggact tctggacgct
     1801 gctgcccgaa gcactgcacc aagttaccat cgtgatgagc gaccgcggca tccccgccgg
     1861 ctaccgccat atgcacggct tcggttcgca tacctacagc ttctggaacg aagcaggcga
     1921 gcgtttttgg gtgaaattcc atttccgcac ccaacaaggc attaaaaacc tgaccaacga
     1981 agaagccgcc aaaatcatcg ccgacgaccg cgaaagccat cagcgcgact tatacgaagc
     2041 catcgaacgc ggcgagtttc cgaaatggac gatgtacatc caagtcatgc ctgaagcaga
     2101 cgcggaaaaa gtaccttatc atccgtttga cttgaccaaa gtttggccga aaaaagacta
     2161 tccgctgatt gaagtgggcg aattcgagtt gaaccgcaat cccgaaaact tcttcgccga
     2221 tgtggaacaa tccgccttcg caccgagcaa cctcgttccc ggtgtcggcg ccagcccaga
     2281 taaaatgttg caagcccgtt tgttcaatta cgccgacgcg caacgctacc gcttgggcgt
     2341 gaacttccgc caaatccctg tcaaccgtcc gcgttgccct gttcacagca accagcgcga
     2401 cggacaagga cgcgccgacg gcaactacgg cagcctgccg cactacgaac ccaacagctt
     2461 cggccaatgg cagcagcagc ccgacttcgc cgaaccgcct ttgaaaatca acggcgacgc
     2521 ggcacactgg gactaccgcc aagacgatga cgactatttc agccaaccgc gcgccctgtt
     2581 caacctgatg aacgacgcgc agaaacaggc attgttcggc aacaccgccg ccgcaatggg
     2641 cgacgcgccc gacttcatca aataccgcca tatccgcaac tgctaccgtt gcgatccggc
     2701 atacggcgaa ggcgtggcaa aagccctcgg actgactgtc gaagacgcac aagccgcccg
     2761 cgcgaccgac cccgcactcg gccagcccgg tttgctgtaa gggggcatta tgtggatgga
     2821 aattgaagaa atcctgtccc gcgccgtccg ctaccggaaa taaccgggca taaaaatgcc
     2881 gtctgaaacg tcgtttgacc gcttcagacg gcatttcccc atcccgcccc cgccgtttca
     2941 gtgggcgttt tttattaaac gcaaaatatc ccgtcattcc cgccccacag cccacttttc
     3001 cgccgtcatt cccgcaccat ccccaccggt tcaaaccggc acgaaaactt ttccgcgtca
     3061 ttcccgcgaa agcgggaatc
//
LOCUS       DAWFBO010000182         2959 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFBO010000182 DAWFBO010000000
VERSION     DAWFBO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.182"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(137..1879)
                     /gene="hrpB"
                     /locus_tag="WGO87_001861"
     CDS             complement(137..1879)
                     /gene="hrpB"
                     /locus_tag="WGO87_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0393177.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISTFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITADLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            complement(1940..2179)
                     /locus_tag="WGO87_001862"
     CDS             complement(1940..2179)
                     /locus_tag="WGO87_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0393178.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTDL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            complement(2463..2765)
                     /locus_tag="WGO87_001863"
     CDS             complement(2463..2765)
                     /locus_tag="WGO87_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393179.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
BASE COUNT          825 a          660 c          629 g          845 t
ORIGIN      
        1 gataatgcga tgtaaacctt tattcatgtc tattccattt tgaagatgaa cgtactgcgc
       61 gccaagtacg taggtaaagt ttgacggtct gaggataagg aaagaccgtc taaatatcag
      121 taaaaaaatt cagaggttag aaactgtaat tcaagttgaa gccgtaaacg gtgttggtcg
      181 tctgaaagcc tttgggttta tgaagcggct tgccggcaaa cagatcataa gcaaacatac
      241 cgcctacttt atgccctcct ctgaagccga ccactgcacc catcagctgc ttgcccgata
      301 catattgtgc actttcgcca gatacgcggc catagtccgc accgagatag aactgatggt
      361 tcggatgaaa ataccaagtt aaagtattct gccagtagaa acctcgctct ccgaaaagac
      421 tctgctcccc atcaaatccg cgaacggtgt agcggctgcc gattgacaat ttatcttggg
      481 caaccaacgg cgttttgttc cattgagctt gaatggcggt tgcgtagaaa aactgctgtt
      541 tgcctaaaat aaatggggcg gctgcgtcca aatcggcagt aatgattttc atacgagatg
      601 tacctggaag aatatcgccg ccgttttctt caggtgcggg catactttgg cgcataccgg
      661 tcccgcgttt gtaagacaac ttgccgtcaa gctgccaacg gttgaggtaa gcacggtggc
      721 gcaattcggc ttcccagcct gcagagcggc ggcgttgcac ttcgatttcg gcatcgtcga
      781 tgtatttata ggtttggcgt gtccataatt tcattccgac tgaagtttta tgaaacctgt
      841 tacgccaaag catgcgctcg gcggccaggc tgctctgata ttgtttgccg ttgtaatcgt
      901 aattgacgga atagccttcg gttgcttcgt ggtaacgatg tccattgtga ttaaaagaaa
      961 acagccattt ttttacgggc accgaataat gcacgctgta acttctggat ccgctttcag
     1021 tttccgtacc ggtggcatca gtcaagtccg ttttgtgcgc caaaccgcgt ccatatgaaa
     1081 cataaaacaa atcgcttaag cccaaagggt tatcgaacga taaagcgaca tttccttgat
     1141 atttgccggt cgttttgccg cccgcatcat ctataccgat actgaaccgt atgggtttat
     1201 tctgctgcca tttgatctgt aaatcgcttt tgccttcttc ttcggacggt ataatctgaa
     1261 tatctgtttt aacactcgac aaacgacgca ggttttccaa gccctgctct acatcgcgaa
     1321 gattgagaat tttgttccta tataagggaa atttgttatt gaatgtacta atactgcctt
     1381 cggcagactt cccatcccgt ttttcttcat agcggatatc ccctatttcg cctgctgata
     1441 cccgtaattt cagaattccc gaatccatat tctgtggttg gataatagct tgggaagtga
     1501 ggtagccacg cacaatcagt atctgttgcg cggctttttg tagcctgctc aaattattgg
     1561 aacctaaaca catcccagtt ttaaaagctg tttctttcat gagcacagaa ggaagaaaag
     1621 aaaatttgcg cgccgtctta tcatctaaac taatgtaatt tacccgagta cacggtgttt
     1681 catcttcact caggacataa ttgttcttct ccaatggttg ctcgaaacgg acatttgcat
     1741 cagttaacaa ttcagcatct atgtgctgct gacgctgcat ggaacggata agttctgcat
     1801 cgttttcatc ggcagctaag gttttaaggg gtatgacagc caggataacc aacagacatg
     1861 gagcaggaaa aaatttcatg acatcaatat tattttagca atatttacta ttttgtcata
     1921 aatttaaaag tatttacagt tatagaatga gacctttgca aaattcccca aaattcccac
     1981 caagacattt aggggatttt ggggaatttt gcaaaggtct cggacagtat tttgaacgca
     2041 gtgcgcgtaa atccgtatgg aaaccatgaa atcccgccac agccgccaga catgccaagc
     2101 cgcattctga tatttctgtt tgcaggataa caggcagctt tttctttaag cccaaagaca
     2161 ggttttgcag atggggcata gatttccttt ttgaaaaata gggattagga agttggatgt
     2221 attttagaaa ggccgcctga aaaggtttca gacgaccttt tgcgactagc tgctatttta
     2281 tttaaagctt ttctctaaca aacgagctaa tatttttcct gtaataaaac agataaaaaa
     2341 cagcatccaa tacgtcagat tggaaaaatc ggtcgtatag agaatcaaca tataaagaag
     2401 cagcatgatg ccgagtgcga tgaattgata atgtttggca aacatcatga cctcctcaac
     2461 tattaaggca aaccgcctga atattctcgt tcaatcgttt cggcaatttc cctataacgt
     2521 cgataccatg accagtcgaa attttcaatg gcatggctcg caaacgtacc aaattcaggc
     2581 atccctatgc ggctacctgc taaagctccg attgtagctc cccaaccagg cggattactg
     2641 aacgtattgt ttggcaatcc taaagattta tcaggatttc ccgtatcgtt aagccctgca
     2701 gattcgcaaa tttcgcaata atatgagctt tgttgcgcat tacctgagcc tccgacccaa
     2761 gtcatttcat gaacacatat agtggattaa atttaaatca ggacaaggcg acgaagccgc
     2821 agacagtaca aatggtacgg caaggcgagg caacgctgta ctggtttttg ttaatccact
     2881 ataaaactct cattttgaaa ctccttgtat cgttaatcaa acaatcaaaa gggcagatgc
     2941 cctatccttg cttttacag
//
LOCUS       DAWFBO010000183         2935 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFBO010000183 DAWFBO010000000
VERSION     DAWFBO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.183"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            162..1274
                     /gene="nadA"
                     /locus_tag="WGO87_001864"
     CDS             162..1274
                     /gene="nadA"
                     /locus_tag="WGO87_001864"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0393180.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNSGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1326..2834
                     /gene="nadB"
                     /locus_tag="WGO87_001865"
     CDS             1326..2834
                     /gene="nadB"
                     /locus_tag="WGO87_001865"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0393181.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLSQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRCPNIRVYERQMALDIQTES
                     GAAYGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDLRAELAPR
                     DIVARDIAAEIAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSQRSSEHPSAEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLHR
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT          685 a          903 c          807 g          540 t
ORIGIN      
        1 caaagcggtt tgtcggaacg aagcgggtcg gttttacggt atcgcaaacc tgttttcaga
       61 cgaccttgct tattatactc aagttgagca taatataaaa cagtttgaaa agcagacggc
      121 aaaccttcat gccgtctgaa aaccagctac aaggaaacat catgcaaacc gccgcccgcc
      181 gctcgttcga ctacgatatg cccctcatcc aaacgccgac ttccgcctgc caaatccgtc
      241 aggcgtgggc gaaggttgcc gatacgcccg accacgagac ggcaggccgt ctgaaagacg
      301 aaatcaaggc tttgctgaag gagaaaaacg cggtcttggt ggcgcattat tacgtcgatc
      361 cgctgattca ggatttggct ttggaaacgg gcggatgcgt gggcgattcg ctggaaatgg
      421 cgcgcttcgg tgcggaacac gaagccggta cgctggtggt ggctggtgtg cgcttcatgg
      481 gcgagagcgc gaaaatcctc tgccctgaaa aaacggtgct gatgcctgat ttggaggcgg
      541 aatgttcttt ggatttgggt tgtcccgaag aagcgttttc agcgttttgc gaccaacacc
      601 ccgaccgcac ggtggtggtg tacgccaaca cttccgccgc cgtgaaagcg cgtgccgact
      661 gggtggtaac gtcttcggtg gcgttggaaa tcgtgtcgta tttgaaatcg cgcggcgaga
      721 aactgatttg gggacccgac cgccacctcg gcgactacat ccgccgcgaa acgggcgcgg
      781 atatgctgtt gtggcagggt tcgtgcatcg tccataacga attcaaaggg caagagctgg
      841 cggcgttgaa ggcggaacat cccgacgcgg tggtgctggt tcatcccgaa tcgccgcaaa
      901 gcgtcatcga actgggcgac gtggtcggct cgaccagcaa actgctcaaa gccgccgtat
      961 cgcgtcctga aaaaaaattc atcgtggcga ccgatttagg catcctgcac gaaatgcaaa
     1021 agcaggcgcc cgacaaagaa tttatcgccg caccgacggc gggcaacagc ggaagctgca
     1081 aaagctgcgc gttctgcccg tggatggcga tgaattcgct gggcggcatc aaatacgccc
     1141 tgacaagcgg gcacaacgaa atcctgttgg acagaaagct gggcgaagcc gccaaactgc
     1201 ctttacagcg tatgctcgac ttcgcggcag gactcaaacg cggggatgtg ttcaacggca
     1261 tgggccccgc ctgatttgcc gtcaatattg ccgtttcaga cgacctctca aacaaggaca
     1321 acaccatgca aaccgattgc gacgtattga ttgccggaaa cgggctggcg gcactgacgc
     1381 tcgccctgtc gctgcctgaa tcgttccgca tcgtcatttt gtgcaaaaac cggctggacg
     1441 acaccgccag ccgtcatgcg caaggcggga ttgcggcggc gtggtcggga gaggacgaca
     1501 tcgaaaaaca cgttgccgat accttggaag ctggcgcggg tttgtgcgac gaagccgccg
     1561 tccgcaccat cctgtcgcag ggcaaaccgg caatcgaatg gctgctgtcg cagggcgtgg
     1621 cgttcgacca aaataataac ggcctgcacc tgacacgcga aggcgggcat acctgccgcc
     1681 gaatcgccca cgtcgccgat tacacgggcg aagccgtcat gcagagcctg attgcccaaa
     1741 tacgccgctg cccgaacatc cgcgtttacg agcggcagat ggcgttggac atccaaaccg
     1801 aatcaggcgc agcgtacgga ctgaccgtcc tcgactgccg aacgcaagaa acctaccgca
     1861 tccgcgcccg ccataccgta ctcgcaggcg gcggcttggg acagatttac gccgccacca
     1921 ccacgccgcc cgaatgcacg ggcgacgcca tcgccatggc aatacgcgca ggctgcgcag
     1981 tcgaaaacct cgaatttatc caattccacc ccacaggctt ggcaaggccg tctgaaaacg
     2041 gacgcacctt cctgatttcc gaagccgtgc gcggcgaagg cggcattctg accaaccaat
     2101 cgggcgaacg gtttatgccg cattacgacc tccgtgccga actcgcaccg cgcgacatcg
     2161 ttgcccgcga catcgccgcc gaaattgcca aacaaacgca agacttcgtc tcgctcgata
     2221 tcagccatca acccgcagcg ttcgtccgtc agcatttccc gtccatccat cggcactgcc
     2281 tgtcccaatg cggcttggac atcacgcgcc aagccatccc cgtccgcccc gtgcaacact
     2341 acacctgcgg cggcatccaa accgaccctt gcggcagaac ctccctgccg cagctctacg
     2401 ccttaggcga aaccgcctgc acagggctgc acggagccaa ccgcctcgcc agcaattccc
     2461 tgctcgaatg cgtcgttacc gccagacttg ccgcccaaac catcgcggac ggacaagcat
     2521 tccaaaccgt accgtcccaa aggtcgtctg aacacccctc cgccgaagca ggcatctttt
     2581 cagacggcat ccaaagcgca ttcagccgcc ccgtcctgca agccttcaac caacgccatc
     2641 tgggcatcct ccgcaacgat accggcctgc accgcgccat cgcccaactg cggctttgga
     2701 agcaaaacca aaccgaaccg cacaccgcgt ccgaatacga aaaccgcaac ctgctcgaat
     2761 gcagcctcgc cgtcgcccaa gccgcataca ggcggagaca gaacatcggc gcgcatttta
     2821 atagtgattg ttaaggttgg ctggttagaa aaaaatcgtt caaataaatt tgaatagcat
     2881 ggttgagaaa ttggcattca gtttttaggg ttgcgtaagt tgggttatca atctg
//
LOCUS       DAWFBO010000184         2902 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFBO010000184 DAWFBO010000000
VERSION     DAWFBO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.184"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            31..1248
                     /locus_tag="WGO87_001866"
     CDS             31..1248
                     /locus_tag="WGO87_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0393182.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTAIAIIGLANIAGCVF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            complement(1451..1591)
                     /locus_tag="WGO87_001867"
     CDS             complement(1451..1591)
                     /locus_tag="WGO87_001867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393183.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1604..2732
                     /locus_tag="WGO87_001868"
                     /pseudo
     CDS             1604..2732
                     /locus_tag="WGO87_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          677 a          862 c          690 g          673 t
ORIGIN      
        1 cgcaaactta accgactcaa aatacacatt atgacgcaca ccgcatcgaa aacacccaaa
       61 ttttggctga ccatcaccgc cgccgcattc atcctgctga ttaccatcgg catgaggatg
      121 acgctcggac tgttcgtcca acccgtcgtc aacaccaccg aattgagcat tgcccaattc
      181 agcctcatca tcgccgtttt ccaactgatg tggggcatat cgcaaccatt gtccggcgcg
      241 cttgccgacc gtttcggcgc gttcagggta ttaagcggcg gcgccgtcct gctcgtctgc
      301 gcctgcctga ttgcccccaa catcccgact tattgggggc tgatgattgc cgtcgggctg
      361 ctgctcgcat tcggcaccgg ttccggcggt ttctccatca ttatggggca ggtcgccgcc
      421 caagtccccg cacacaaacg cggcttggca tccggactgg tcaacgccgg cggttcggca
      481 ggacaattcc tgttcgcacc gctggttcaa ggactcgtcg tcctgcccga agtcggctgg
      541 acgggcacat tttacgtttg gggcgcaatc gccctgctga ttctgcctgt ctcatggtgg
      601 cttgccggcg gcaacaacgc cgcccacacc caacacgccc aagccacaca cggacaaagc
      661 ctcggagaag cagtcaaaac cgccttcaaa accccaagct acatcctgct gcacctaagc
      721 ttttttgcct gcggcttcca catcgccttt ctcgtaaccc acctacccac ggaagtcgcc
      781 ctgtgcggac tgcccgccac cgtcgcatcc acagccatcg ccatcatcgg actggcaaac
      841 atcgccggtt gtgtgttctc aggctggtgc acaagccgcg ttcggggcaa atacgtcctg
      901 ttcggactgt acgcctcgcg cgccgcgatg atactcatct acattttttc gcccaagacc
      961 gacctcaact tctacatttt cgccgccgca ctcggattta cctggctcgc caccgtcacg
     1021 ccgaccgcct ccattaccgg caaactcttc ggcacacgct acctcgccac cctgttcgga
     1081 ctgacgatgc tcagccacca aatcggcgga ttcctcggct cgtacatcgg cggcatcgtg
     1141 attacacaat tcggcgacta cggctggatg tggtacgccg acgcactgct ggcgggaacc
     1201 gccgccctcc tcaacctccc catccgcgaa ccccgaaccg ccgcatgaaa gaatgccgtc
     1261 tgaaaaccct cagacggcat tttccaaaca ccccgcccgt ttccggactg cacgacacgc
     1321 aaatcagcct tatcaaaccg caaacaaccg ccgcgattaa accgccattc aaataagtcc
     1381 gttttccctt cgggtgtaaa atcatcacaa aacgcgatac tgtatatcgc cgggcgttat
     1441 gcagcggatt tcaggataaa atgccgtctg aagccgcccc tgcccgggcg ggcgaagcgt
     1501 cggatgcggt gcgcttggca aaggtttgcc cgccgcgccc cgtatccggc atttcatcaa
     1561 tgcagaccgc atttccaatc ctataattca aggtgtccgc cttatgaaaa tcgtttttat
     1621 cacaacagtc gcatccagca tttacggttt ccgcgccccc gtcattaaaa aattaatcgg
     1681 caaaaaccat caggtgtatg cctttgtatc ggagttttcc gataatgaat tggatattat
     1741 cagggaaatg ggggttacac ccgttaccta ccgttcaaac cgcagcgggc tgaacccgtt
     1801 ttccgatata aaatccacct tcctcatctt taaagaactc aaaaaaatat cgccggattt
     1861 ggttttccct tatttcgcaa aacccgtgat tttcggcact tttgccgcaa aactggcagg
     1921 cgtgcccaga atcgtcggga tgctggaagg tttgggattc gcatttaccc cgcagccgga
     1981 aggcataccg ttaaaaacaa aaatcataaa ggggattttg attgccttgt accacattgc
     2041 cctgccgatg ttggaaagcc tgattgtatt aaaccccgac gacaaagacg aactgacgga
     2101 caaatacggc atcaaaataa aaaacatcca tattttgggc ggaatcggtc tggatttgcg
     2161 gcaatatcct tattccgagg cggatattcc cgatgaaaaa gaacccgtaa aatttctctt
     2221 tatcggcaga tttctgaaag aaaaagggat tgatgatttt attcgggcgg cggaacaggt
     2281 taagggcaaa taccccgata cggtttttac cgctttgggc gcaatcgaca aatcacgcgg
     2341 gggggggggg cgatttggaa cggcttgccg cccgcgatat tatccgtttc cccggttttg
     2401 tgaacaatgt ttccgaagtg ataaaggcgc atcatatatt cgtattgccg tcttataggg
     2461 aaggcgttcc ccgaagcact caggaggcaa tggccgtcgg cagggcggtg attacgacgg
     2521 atgtccccgg atgcagggaa acggtcgccg acaaggtcaa cggcttcctg atcgaacctt
     2581 ggaatccccg catcttggcc gaaaaaatga tttattttat cgaaaacagg gaagccgtcc
     2641 gcctgatggg gaatgcaagt tatgcgattg ccaaagataa attcgatgcc gaaaaagtcg
     2701 atttgaaatt gctcgatatt ttgaaggcgt aaacaaggct gcccgctttt gagtttcggt
     2761 catttctgat aaacccccgt cattcccgcc acacccccgt cattccctcg aaagcgggaa
     2821 ttcaggttcg tttagtttcg gtcatttccg ataaattctt gcaactttgc gtttctagat
     2881 tcccactttc gtgggaatga cg
//
LOCUS       DAWFBO010000185         2867 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFBO010000185 DAWFBO010000000
VERSION     DAWFBO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.185"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(81..557)
                     /gene="greA"
                     /locus_tag="WGO87_001869"
     CDS             complement(81..557)
                     /gene="greA"
                     /locus_tag="WGO87_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0393184.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            952..2253
                     /gene="aroA"
                     /locus_tag="WGO87_001870"
     CDS             952..2253
                     /gene="aroA"
                     /locus_tag="WGO87_001870"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0393185.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     ENYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPKTLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2296..2829)
                     /locus_tag="WGO87_001871"
     CDS             complement(2296..2829)
                     /locus_tag="WGO87_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0393186.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          604 a          791 c          771 g          701 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat aatttcctgc gtgtgtcggg
       61 tgtatcgaaa tcaagccgaa tcaaatatat cggacttcga taatgtcgta ttcgcgcacg
      121 ccgcccgggg cttggacttc cgccgtatcg ccctcttcct tgccaattaa ggcgcgggca
      181 atcggtgagc cgacatagat tttgccctgt ttgatatccg cttcgtcttc gccgacaatt
      241 tggtaggtaa cgtgttcttc cgtttccaaa tcttccagcg taaccgtcgt accgaacacg
      301 attttgcctt cggcgtggat ttcggtcgga ttgatgatgt gggcaacgga aagtttgtgt
      361 tccaactcgg aaatgcggcc ctcgataaaa ccttggcgtt ctttggcggc ttcatattcg
      421 gcgttttcgg acaaatcgcc gtgcgatctg gcttcagcaa tcgcttcgat cacttcgggg
      481 cgcgccacgc ttttaagctg ctgcaattcc tgtttcagca attccgcacc gcgtacggtc
      541 agggggattt tttgcatcgg tcttttttct ccatattccg gcacaccggt ttgcggcagc
      601 aaacataccg cataccgtct tgttttgtgc gtccggatat taaaataaaa acacaagccg
      661 cccggaaaat cggcggcttg tctgtcgttg aacagcggct attctaccaa attctatgaa
      721 attggcaatc gtaccgtacc gtccgcaaac gtcatctgtc cgcaacaaaa gccgaaaata
      781 tgccggcaaa aaaattttag aaacaaaaaa tttaaaaata atcaattttc ggcataaaaa
      841 accacattta cggactttaa aaccgaaaat gccaaacctg agatttttca tacagcattt
      901 gcaccagtat aatgcaggct gtttttatct ttataatatt gacgttttgc catgaccgaa
      961 tccctccgcc tccctgccgc ctcgctcaaa ccttccactg tcgccctgcc cggctccaaa
     1021 agcatcagca accgcaccct gctgcttgcc gccttgtccg acaatgcttg cgaaatccat
     1081 tccctgctca aatccgacga taccgaccgt atgctcgaag cactcgataa actcggcgtt
     1141 gaaatcgaat atcttgccga agaccgtctg aaagtgcacg gcacaggcgg acgcttcccc
     1201 aaccgcactg ccgatttgtt tttgggcaac gcgggcacgg cgttccgccc gctgaccgcc
     1261 gcgcttgccg ttttgggcgg cgattatcgc ctgcacggcg ttgcgcgtat gcacgaacgc
     1321 cccatcggcg atttggccga tgcactgcgg attgccggcg ccgatgtcga atatctcggc
     1381 aaggaacact atccgccgct tcatatcggc gaacgccaag acaacggcga gcgcgtgatt
     1441 ccgattaaag gcaatgtgtc cagccagttt ctgaccgccc ttttaatggc gttgccgctg
     1501 accgggcagg cgtttgaaat ccgtatggtc ggcgagttaa tttccaagcc ctatatcgac
     1561 attactttaa aactgatggc gcaattcggc gtacaggttg ccaatgaaaa ctaccgcgtc
     1621 ttcaaaattc ccgccgatgc gcactaccac gcgcccgaac acttgcacgt cgaaggcgat
     1681 gcctccagcg cgtcctactt cctcgcagcc ggtttgattg ctgccacgcc cgtgcgcgtg
     1741 accggcatcg gcgcaaacag catacagggc gatgtcgcct ttgcccgcga gctggaaaaa
     1801 atcggggcgg acgtggtttg gggcgaaaac ttcgtcgaag tttcacgccc gaaggaacgt
     1861 gccgtccaat cctttgattt ggatgcgaac catatccccg atgccgccat gaccctcgcc
     1921 atcgtcgcgc ttgctacagg gcaaacctgc acgctgcgca acatcggttc gtggcgcgtc
     1981 aaagaaaccg accgcatcgc cgcgatggca aacgagttgc gcaaactcgg ggcaaaagtc
     2041 gtcgaagaag ccgaagcaat tcacatcacc ccgcccaaaa cgctgacacc cgacgccgtc
     2101 atcgacacgt acgacgacca ccgcatggcg atgtgtttct cgctggtttc gctgttgggc
     2161 gtacccgtcg tcatcaacga tccgaaatgc acccataaaa ccttcccgac ttatttcgac
     2221 gtgttctctt cactgaccga aacaacggaa taaggcggca ttttgccgcg attccggcgc
     2281 ggcgcggcgg gcggctcatt ctgtaaagaa agtatgtgcg ccgaggtagt ttttggcgta
     2341 aaacggtgtg gagagttttt cggttttgat ggttttgccg ctgctggggg catggatgaa
     2401 ttcgccgttg ccgatataga gtccgacgtg tgagtagcgg tgtgtgccgc cggtgttgaa
     2461 gaatacgagg tcgccggcct taaggcggct gtcggggatt ttgcggcttg ccgccgccat
     2521 gtcgcgggcg gtgcgcggca gcttgacgtt gagggcgttt ttgtaaacga attgaatcat
     2581 gccgctgcaa tcgaagccgg ttgcggtgct gctgccgccc catttgtagg gcgtgccgat
     2641 gagtccgagg ctgtggagca tgagttcctg cgagccttgt gtgcggtcga tgtggctgat
     2701 gcggacggct tggatttgcc ggactgtctg tttgggtttc ggttggcggt gtttgccgga
     2761 ggtcgtgccg catgaggcga ggagcagtgc gctgagacag aggaaaaggg ttttgtcggg
     2821 gggaaacatg gtttttcctt tgcgggttcg gatatccgtc tgaaggt
//
LOCUS       DAWFBO010000186         2792 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFBO010000186 DAWFBO010000000
VERSION     DAWFBO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.186"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            150..473
                     /locus_tag="WGO87_001872"
     CDS             150..473
                     /locus_tag="WGO87_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0393187.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            587..2764
                     /locus_tag="WGO87_001873"
     CDS             587..2764
                     /locus_tag="WGO87_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0393188.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          672 a          994 c          707 g          419 t
ORIGIN      
        1 cccctctccc tgtgggagag ggttagggag agggcggtaa gccgcaggct tacatcaaag
       61 ccgataacgc tttcgttaca actccgccca ctgaacgaag ccaaaacgac aaaccgcatc
      121 gtaaaccacc caacccatag gagaacccca tgcaaaacga aaccatcaac ctgaaacagc
      181 accttgccgc catcaaagaa tactggcagc ccgaaatcat caaccgccac gggttccaat
      241 tccgtttggt caaacttttg ggcgattacg gctggcatac gcacggatac agcgacaaag
      301 tgctgtttgc cgtggagggc gacatggcgg tggacttcgc cgacggcggc agcatgacga
      361 tacgcgaggg cgagatggcg gtcgtgccga agtcggtgtc gcaccgcccg cgttcggaaa
      421 acggctgctc gttggtgctg attgagttgt ccgacccgtc cgaggccgtc tgaaaacgaa
      481 gtttccgaag gaagctgagt ttctgcgaag ctaaaagcag cctgcacctt caatcaatat
      541 gccgaaaata caaccccacc gcacaccaac acacaaagga aatcccatga cacgcttcaa
      601 atattccctg ctgtttgccg ccctgttgcc cgtgtacgcg caggccgatg tttctgtttc
      661 agacgacccc aaaccgcagg aaagcactga attgccgacc atcaccgtta ccgccgaccg
      721 caccgcgagt tccaacgacg gctacactgt ttccggcacg cacaccccgc tcgggctgcc
      781 catgaccctg cgcgaaatcc cgcagagcgt cagcgtcatc acatcgcaac aaatgcgcga
      841 ccaaaacatc aaaacgctcg accgcgccct gttgcaggcg accggcacca gccgccagat
      901 ttacggctcc gaccgcgcgg gctacaacta cctgttcgcg cgcggcagcc gcatcgccaa
      961 ctaccaaatc aacggcatcc ccgttgccga cgcgctggcc gatacgggca atgccaacac
     1021 cgccgcctat gagcgcgtag aagtcgtgcg cggcgtggcg gggctgctgg acggcacggg
     1081 cgagccttcc gccaccgtca atctggtgcg caaacgaccg acccgcaagc cattgtttga
     1141 agtccgcgcc gaagcgggca accgcaaaca tttcgggctg ggcgcggacg tatcgggcag
     1201 cctgaacacc gaaggcacgc tgcgcggccg cctggtttcc accttcggac gcggcgactc
     1261 gtggcggcag cgcgaacgca gccgcgatgc cgaactctac ggcattttgg aatacgacat
     1321 cgcaccgcaa acccgcgtcc acgcaggcat ggactaccag caggcgaaag aaaccgccga
     1381 cgcgccgctc agctacgccg tgtacgacag ccaaggttat gccaccgcct tcggcccgaa
     1441 agacaacccc gccacaaatt gggcgaacag ccgccaccgt gcgctcaacc tgttcgccgg
     1501 catcgaacac cgcttcaacc aagactggaa actcaaagcc gaatacgact acacccgcag
     1561 ccgcttccgc cagccctacg gcgtagcagg cgtgctttcc atcgaccaca acaccgccgc
     1621 caccgacctg attcccggtt attggcacgc cgacccgcgc acccacagcg ccagcgtgtc
     1681 attgatcggc aaataccgcc tgttcggccg cgaacacgat ttaatcgcgg gtatcaacgg
     1741 ttacaaatac gccagcaaca aatacggcga acgcagcatc atccccaacg ccattcccaa
     1801 cgcctacgaa ttttcccgca cgggtgccta cccgcagcct gcatcgtttg cccaaaccat
     1861 cccgcaatac ggcaccaggc ggcaaatcgg cggctatctc gccacccgtt tccgcgccgc
     1921 cgacaacctt tcgctgatac tcggcggcag atacagccgt taccgcaccg gcagctacga
     1981 cagccgcaca caaggcatga cctatgtgtc cgccaaccgt ttcaccccct acacaggcat
     2041 cgtgttcgac ctgaccggca acctgtctct ttacggctcg tacagcagcc tgttcgtccc
     2101 gcaatcgcaa aaagacgaac acggcagcta cctgaaaccc gtaaccggca acaatctgga
     2161 agccggcatc aaaggcgaat ggcttgaagg ccgtctgaac gcatccgccg ccgtgtaccg
     2221 cgcccgtaaa aacaacctcg ccaccgcagc aggacgcgac ccgagcggca acacctacta
     2281 ccgcgccgcc aaccaagcca aaacccacgg ctgggaaatc gaagtcggcg gccgcatcac
     2341 gcccgaatgg cagatacagg caggttacag ccaaagcaaa acccgcgacc aagacggcag
     2401 ccgcctgaac cccgacagcg tacccgaacg cagcttcaaa ctcttcactg cctaccactt
     2461 tgcccccgaa gcccccagcg gctggaccat cggcgcaggc gtgcgctggc agagcgaaac
     2521 ccacaccgac cctgccacgc tccgcatccc caaccccgcc gccaaagccc gcgccgccga
     2581 caacagccgc caaaaagcct acgccgtcgc cgacatcatg gcgcgttacc gcttcaatcc
     2641 gcgcgccgaa ctgtcgctga acgtggacaa tctgttcaac aaacactacc gcacccagcc
     2701 cgaccgccac agctacggcg cactgcggac agtgaacgcg gcgtttacct atcggtttaa
     2761 ataaggtcgt ctgaaaacgg agtttctgcg aa
//
LOCUS       DAWFBO010000187         2583 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFBO010000187 DAWFBO010000000
VERSION     DAWFBO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.187"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            32..511
                     /locus_tag="WGO87_001874"
     CDS             32..511
                     /locus_tag="WGO87_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0393189.1"
                     /translation="MPSETAVADIARTYSAHYLNPNNRNMTLFPDALPCLDKLKAQGY
                     WLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGFDPKEA
                     LVVGDTAHDLHMAANAGAAAVGVATGAHSREQLRTAPHLAVLDGLSELPGFLAQHYA"
     gene            complement(598..1566)
                     /gene="ispH"
                     /locus_tag="WGO87_001875"
                     /gene_synonym="lytB"
     CDS             complement(598..1566)
                     /gene="ispH"
                     /locus_tag="WGO87_001875"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0393190.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAEERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1595..2092)
                     /locus_tag="WGO87_001876"
     CDS             complement(1595..2092)
                     /locus_tag="WGO87_001876"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0393191.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKH"
     gene            complement(2298..2402)
                     /locus_tag="WGO87_001877"
                     /pseudo
     CDS             complement(2298..2402)
                     /locus_tag="WGO87_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          589 a          790 c          621 g          583 t
ORIGIN      
        1 cctgcccgaa atcatccgcg acctgctcga aatgccgtct gaaaccgccg ttgccgacat
       61 cgcgcgcact tattccgcgc attacctcaa tcccaacaac cgcaatatga cgctctttcc
      121 cgatgccctg ccctgtctgg acaagctcaa agcacaagga tactggcttg ccgtcgccac
      181 gggcaaaggg cgggcgggct tggacaacgc catcagccaa accgccaccg gcggctattg
      241 gctcgccacc gcctgcgcgg gggaatatcc gtccaaaccc tcgcccgaaa tggtattcgg
      301 actctgcggc gaactggggt tcgacccgaa agaggcattg gtcgtcggcg atacggcgca
      361 cgacctgcat atggcggcaa acgcaggcgc ggcggcagtc ggcgtggcca ccggcgcaca
      421 ttcgcgcgaa cagctccgca ccgcaccgca tctcgccgta ttggacggtt tgtccgaact
      481 gcccggtttt cttgcacagc attacgcctg attggtttcc gcatcagaca ctcaaaacaa
      541 atgccgtctg aagcctgttc agacggcatt tgtgttgccc aaacattcaa cgcttgctca
      601 acgtttgcac aaatcgggtt tggtttcgcc ctcgcggcgc agctctttgg gcaggacgaa
      661 cacaatgctt tcttccgcac cctcgccttc gcgtacggtt tcgtgccccc atccgcgtat
      721 ggttgccagt acttcccgca ccaacacttc gggcgcggac gcgcctgccg ttacgccgac
      781 tttgtttttg ccctcaaacc atgcacgttg caggtagcct gcattatcca ccatatacgc
      841 atcgattccg cgcgatgccg ccacttcgcg caagcggttg ctgttggacg aattgggcga
      901 accgaccaca atcacgatgt cgcactgttc cgccaactct ttgacggcgg tttgccggtt
      961 ggtcgtcgca tagcagatat cttccttgtg cggattgcgg atattgggga aacgcgcgtt
     1021 cagcgcggcg atgatgtctt tggtttcatc gaccgagagc gtggtttggc tgacataggc
     1081 gagtttgtcg gggtttctga cttcgagttt tgccacatct ccgaccgttt cgaccaaaag
     1141 cattttgccc ggcgcaagct gccccatcgt gccttcgacc tcgacgtgcc ccttatgccc
     1201 gatcatgatg atttcacagt cttgggcatc cagtcgggcg acttccttat gcactttcgt
     1261 caccagcggg caagtcgcat caaacacgcg gaaaccacgt tcctccgctt cttgctgcac
     1321 cgccttcgat acgccgtgtg ccgaataaac cagtgtcgcg cccggcggca cttccgccaa
     1381 gtcttcaata aacaccgcac ctttttcacg caggttgtcc acgacgaatt tgttgtgaac
     1441 gacttcgtgg cgcacataaa tcggcgcgcc gaactcttcc aaagcacgtt cgacaatact
     1501 gattgcccga tccacaccag cgcagaagcc gcgcggattg gcaaggatga tggttttctc
     1561 gttcataagc ccggtatttc gttttcagac ggcatcaatg tttttcttct tgggttttac
     1621 ggtggacgat gttgtccaac actgccaaca ccgcaccgac gcagataaag ctgtcggcaa
     1681 tattaaaggc gggataaaac caattttgcc aataaaacaa taagaaatcg acgacatgac
     1741 cgtgtatcag gcggtcgatg acattgccta acgcaccgcc gataatcatc gccgcacccg
     1801 ttttgccgag ggttgcaaac tcatcgcgca agatggcgcg taccaaatac gcgctcaccg
     1861 ccaccgccag caccaaaaaa aagtattttt gccagccgcc ctgatcggca aggaagctga
     1921 acgccgcgcc cgggttgtac accaaggtca gatcgaaaaa cgaaggaatg acattgacgc
     1981 gttcccgata ctgaaacgac gacagcaccg cccacttcga ccactggtcc agcacgatgg
     2041 cggcaagtgc caatacccaa tagcgcgttt tacttgaaac agatgaagac atatttttca
     2101 acagccggta aaagagtacc attttacccg aaaaccccct ttcctgtacc cgaaacggca
     2161 aatgccgtaa tcttaaaacc cgtcattccc gacaacaccg taatctcgaa acccgtcatt
     2221 cccgcgtagg cgggaatcca gacctgtccg cacagaaact tatcggataa aaacagttgc
     2281 ccaaaccccg cgttctatag tggattaaat tcaaaccagt acggcattgc ctcgccttgc
     2341 cgtactattt gtactgtctg cggcttcgtt gccttgtcct gatttaaatt taatccacta
     2401 tagattccca cttccgtggg aatgacggtt cagttgcatt ccgacaacac cgtaatcttg
     2461 aaatccgtca ttcccgcgca ggcgggaatc tatcggaaat gactgaaacc tcgagattct
     2521 agattcccac tttcgtggga atgacggttc agttgcgttc caacaacacc gcaatctcga
     2581 aat
//
LOCUS       DAWFBO010000188         2581 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFBO010000188 DAWFBO010000000
VERSION     DAWFBO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.188"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(197..268)
                     /locus_tag="WGO87_001878"
     tRNA            complement(197..268)
                     /locus_tag="WGO87_001878"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Arg,seq:cct)
     gene            complement(288..734)
                     /locus_tag="WGO87_001879"
     CDS             complement(288..734)
                     /locus_tag="WGO87_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0393192.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(739..1713)
                     /gene="ispB"
                     /locus_tag="WGO87_001880"
     CDS             complement(739..1713)
                     /gene="ispB"
                     /locus_tag="WGO87_001880"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0393193.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVAVISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1937..2245
                     /gene="rplU"
                     /locus_tag="WGO87_001881"
     CDS             1937..2245
                     /gene="rplU"
                     /locus_tag="WGO87_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0393194.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2270..2542
                     /gene="rpmA"
                     /locus_tag="WGO87_001882"
     CDS             2270..2542
                     /gene="rpmA"
                     /locus_tag="WGO87_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0393195.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          756 a          696 c          574 g          555 t
ORIGIN      
        1 cataaaacaa cagatttcca agccggaaca tcttttacgg agcctgaaaa tcatttcatg
       61 attctaccgt cctaaaggtc ggtttttcaa gcaggaagaa aaagttttca gatggcaaaa
      121 aagccctcca acactgaaag gccttatatc ggaaacttcc cgcaacacgg gaaacagaca
      181 aatgcaaatc gtcaaacctc gccaacagga atcgaacctg tattttacgc ttaggaggca
      241 tacgttctat ccgttgaact atggcgagcc gaaatgaaaa ggagatttta accctttccg
      301 acgacaaaag acaagatgcc ggccgcaatc agcggcccga cagggatacc gcccataaat
      361 gccaccccga taaccgtccc gattaacagc cctgtaatta aaacaggctg ctgtcccatc
      421 aaaggcacgc cgcgtcccgc cagccaagcc acgaaaatac cgataaaaac ggcggatatc
      481 attttaaaat tcaaaaattc ggcaacggga ggaacctgcg cctttcctga aaccaacgga
      541 ctcaaaaccc ctatggtcaa aagaatgata ccgagattca acccgtgctt ctcgaccaac
      601 gggacaaact gtaccaatgc cgtctgctgc atcagcagca atatggttgc cgagatggta
      661 atcgaattgt tgttgctgac cacccccaac agaatcagcg taaccagaaa caggggaaca
      721 aaactgaaat tcatcgcctc aagacaccct gaccaaagat tccttcgcca gctgaatcat
      781 ggcatccttc acttcgctgt cgggcagggc atccaacgcg gcaacggcac aatcgactgc
      841 tttgcgcgcc tcgcctatcg aatatgccaa agcatccgaa cggacgacat aatcgtggat
      901 tttctcaaaa tagctgcgat ctgcattttc caaagcagta cgcacatcgt tcgcaacctg
      961 ttcggaaccc tgacgcatca gataaatcaa aggcaaagtc ggttttcctt ccgccaaatc
     1021 gtcgccgacg tttttgccgg tttcttcggt ttcgccagaa tagtccagca catcgtcaat
     1081 aatttggaat gccgtaccga catacatacc gtagtctttc aacgcccgtt cgtgttcggg
     1141 ggaagccttg cccaaaattg cgccgacttg agcggcagct tcaaacaatt ttgccgtttt
     1201 atattggatg acttggatat attgttcttc ggtaatgtcc gtattgccga tgttcatcag
     1261 ctgcatgact tcgccctcgg caataatgtt ggttgcatcc gccatcactt ccaaaacgcg
     1321 catactgccc gaggcaacca tcagttgaaa ggcgcgcgtg tataaaaagt cgccaaccaa
     1381 cactgccgcc gcattgccga acagattgtt tgccgttgcc cgcccacggc gcaaatcgct
     1441 ttcatcgacg acatcgtcgt gcaggagggt ggaagtgtgg ataaactcga ccatcgccgc
     1501 cagcgaatac agtttctcgt catcataacc gaccgcctta cccgccaaaa tcgtcataat
     1561 cggacgcagg cgtttgccgc ccgcgctgat gatatatgta ccgatttgcg aaatcactgc
     1621 gacatcggat tgcaccgcac ggttgatgac ttcattgact ttggcaaggt cttcaggcag
     1681 atgtcgctgg aaatagggca gattctcgag catggcagac tctcaggttt acgtttccgt
     1741 caaacggcag ataccgcttg aaagctgatt caaatagcgg gcaattatat cagcaaagct
     1801 atgaggacac atcttttcag acggcatatg gttatccgaa ccgcgcaaac tttgacaaaa
     1861 caggcaaata acaatacaat gccgagttcc gcacaatggt gtgcggatat taaccataac
     1921 ccttatggag ttgagtatgt acgcggtcgt aaaaaccggc ggcaaacagt ataaagtttc
     1981 cgtcggcgaa aaattgaaag tagaacagat accagccgaa ctcgacagcc aaatcgaact
     2041 gaccgaagtt ttgatgattg ctgacggcga atctgtaaaa gttggcgcac cctttatcga
     2101 aggtgcaaaa gtaacggcta aagtagtggc acacggtcgt ggcgaaaaag tccgcatctt
     2161 caaaatgcgc cgccgcaaac actaccaaaa acgccaaggc caccgccaaa atttcaccca
     2221 aatcgaaatc gtggcaatcg cctaacctta aaacttagga gcattacaaa tggcaagtaa
     2281 aaaagcaggc ggcagcaccc gcaacggtcg cgattcagaa gccaaacgct tgggcgttaa
     2341 agcctacggc aacgagctga ttcccgcagg ttccatcatc gtacgccaac gcggtaccaa
     2401 attccacgca ggcgacaacg taggtatggg caaagaccac actttgttcg ccaaagttga
     2461 cggttatgtc gaattcaaaa ccaaaggcgc gctgaaccgt aaaactgtca gcatccgtcc
     2521 ttacactggt tctgaagaat aatccaaccg atacctgaag ccgcatcttt tcacgatgcg
     2581 g
//
LOCUS       DAWFBO010000189         2580 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFBO010000189 DAWFBO010000000
VERSION     DAWFBO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.189"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            75..704
                     /locus_tag="WGO87_001883"
     CDS             75..704
                     /locus_tag="WGO87_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0393196.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            850..2511
                     /locus_tag="WGO87_001884"
     CDS             850..2511
                     /locus_tag="WGO87_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0393197.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          507 a          653 c          671 g          749 t
ORIGIN      
        1 atggggttga agttgacatc tacaaagttt aatgtttgaa tacattaaaa aaatatattt
       61 gaatgagatg ttttatgatt caaaatgttg ttacttcaat aatcctgtat tctgggacag
      121 ccgtagactt acttattatc ctaatgttat tttttgccaa aagaaaaagc agaaaagaca
      181 tcattaacat ctatttagga caatttctag gctctgttag cctgatattg ctaagtttgc
      241 tttttgcatt tgtcttagat tatattccta gtaaagagat tttaggtttg ctcggcttga
      301 ttccaatttt cctaggcatc aaagttttgc tgttaggaga ttctgatgga gaggctattg
      361 ccaaagaggg tttgcgcaaa gataataaaa acctgatttt tctagtcgct atgattactt
      421 ttgcaagttg tggtgctgac aatattggtg tctttgttcc atattttact accttaaatt
      481 tagcgaattt gatagtggct ttacttacct ttctagtcat gatttatctc ttggtttttt
      541 ctgcccaaaa attagcacaa gtcccttctg ttggagaaac tttggaaaaa tatagcagat
      601 ggtttgttgc tgttgtttat ttgggattgg gggtatatat cctgattgaa aacaacagtt
      661 ttgatatgct atggactgtg tcgggccagg aaaaaatatt atgaaaaaga tagtatctgc
      721 aaagactgcc atgtgcagtc ttttttgttg ccggtctttt tgtgtctgat gccgtctgaa
      781 gcagtctctg cacgaccttt gtgcgaatat ttgctacact tggcaatccg tccatttctt
      841 ctcacgctta tgctgaccta cactccgccc gatgcccgcc cgcccgccaa aacccacgaa
      901 aagccgtggc tgctgctgtt gatggcgttt gcctggttgt ggcccggcgt gttttcccac
      961 gatttgtgga atcctgccga acctgccgtc tataccgccg tcgaagcact ggcaggcagc
     1021 cccacccctt tggttgccca tctgttcggt caaaccgatt tcggcatacc gcccgtgtat
     1081 ctttgggttg ccgccgcgtt caaacatttg ctgtcgccgt gggcagccga cccgtatgat
     1141 gccgcacgct ttgccggcgt gtttttcgcc gttgtcggac tgacttcctg cggctttgcc
     1201 ggtttcaact ttttgggcag acaccacggg cgcagcgtcg tcctgattct catcggctgt
     1261 atcgggctga ttccagttgc ccatttcctc aaccccactg ccgccgcctt tgccgccgcc
     1321 ggactggtgc tgcacggtta ttctttggct cgccggcgcg tgattgccgc ctcttttctg
     1381 ctcggtacgg gctggacgct gatgtcgttg gcagcagctt atccggcagc atttgccctg
     1441 atgctgccct tgcccgtact gatgtttttc cgtccgtggc aaagcaggcg tttgatgttg
     1501 acggcagtcg cctcacttgc ctttgccctg ccgcttatga ccgtttaccc gctgctcttg
     1561 gcaaaaacgc agcccgcgct gttcgcgcaa tggctcgacg atcacgtttt cggtacgttc
     1621 ggcggcgtgc ggcacgttca gacggcattc agtttgtttt actatctgaa aaacctgctt
     1681 tggtttgcat tgcctgcgct gccgctggcg gtttggacgg tttgccgcac gcgcctgttt
     1741 tcgaccgact gggggatttt gggcattgtg tggatgcttg ccgttttggt gctgcttgcc
     1801 gtcaatccgc agcgttttca ggataacctc gtctggctgc ttccgccgct tgccctgttc
     1861 ggcgcggcgc aactggacag cctgaggcgc ggcgcggcgg cgtttgtcaa ctggttcggc
     1921 attatggcgt tcggactgtt tgccgtgttc ctgtggacgg gctttttcgc catgaattac
     1981 ggctggcccg ccaagcttgc cgaacgcgcc gcctacttca gcccgtatta cgttcccgac
     2041 atcgatccca ttccgatggc ggttgccgta ctgttcacac ccttgtggct gtgggcgatt
     2101 acccgcaaaa acatacgcgg caggcaggcg gttaccaact gggcggcagg cgttaccctg
     2161 acctgggctt tgctgatgac gctgttcctg ccgtggctgg acgcggcgaa aagccacgcg
     2221 ccggtcgtcc ggagtatgga ggcatcgctt tccccggaat taaaacggga gctttcagac
     2281 ggcatcgagt gtatcgacat aggcggcggc gacctgcaca cgcggattgt ttggacgcag
     2341 tacggcacat tgccgcaccg cgtcggcgat gtacaatgcc gctaccgtat cgtccgcttg
     2401 ccccaaaatg cggatgcgtc gcaaggctgg cagacggttt ggcagggtgc gcgcccgcgc
     2461 aacaaagaca gtaagttcgc actgatacgg aaaatcgggg aaaatatata aaaaacaaca
     2521 gattgagccg aatttctgga ttaagtgccg gaaatcgcgt ataattgcgc gattaaacct
//
LOCUS       DAWFBO010000190         2572 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFBO010000190 DAWFBO010000000
VERSION     DAWFBO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.190"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..1134)
                     /locus_tag="WGO87_001885"
     CDS             complement(<1..1134)
                     /locus_tag="WGO87_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEZ0393198.1"
                     /translation="MRHSHYFQWLSLPLLSVAVTQQLYAQPNESLPTVELEPVVITID
                     KSGMALANRITQMPHTTKVIYEEQIQEQATGSRQLADVMAQLIPSLGVSSGTTSNFGQ
                     TMHGRQVQFLLNGVPLTGSRDISRQLNSINPNQVARIEVLSGATSIYGSGATGGLINI
                     VTKSDLEEEQFETRIGVHGSKLSSEGIGYQVGQSVAGVSENGNVLARLDVDYRTTGGA
                     FDANGKRIAPEPAQTDKQDSKSLSVNTNVDWQLDDKQNINLALTHYNDKQDTDYAPDY
                     GNRLAVLFEEKPSLNAIKGLSLSEQPKTTKSTFNINYHHDDLWGNTINTNAYYRREKG
                     RFYPFVAPFSTAKALPILQSMNLPPATLDAYRAYGVLQSESKAE"
     gene            complement(1181..2437)
                     /locus_tag="WGO87_001886"
     CDS             complement(1181..2437)
                     /locus_tag="WGO87_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0393199.1"
                     /translation="MIISQQKNRHLLTMVALLYVAHALPLYFFNVALPAILRHQGVDI
                     RVIGMLSLLYLPWAFKFLWASYVDNYYCAKIGKRKTWLFFTQIIVVVGLFGLGFLQFS
                     ESEIVLFLSISFLVSLASATQDIAIDGYTVEQFDKSSFGLASAMQSFGVAFGSMVGGA
                     GTLWLYQRFDWQMATISLATTVVLISCSLWFLKDQDNPKHATNLSRASLIRLIKRPQT
                     KWLLALIVVFRFVEAPMMAMLNPMLVDFGFSLSQIGLLFSVFGAIVGIGSALLAGVLI
                     KYIDIVRCLILFGWLRTAVYALLCLGLLYPLLGDFLPAVGLLKTGFMSLMAISVLLIL
                     SVRYLTMTALYALFMNAANPDQAGTDFTLFVCVELLVYFIGGAMSGFLVHQFGYDKLF
                     LWLAIGSGVSMLILPTLIKKFSVTDC"
BASE COUNT          819 a          588 c          482 g          683 t
ORIGIN      
        1 ctctgcctta gattcagatt gtaacacccc ataggcgcga taagcatcca aagtggctgg
       61 tggcaaattc atgctttgta aaataggcag ggctttggcg gtcgaaaacg gggcaacaaa
      121 gggataaaat ctgcctttct ctctgcgata ataagcattg gtattgatgg tgttacccca
      181 caaatcatca tgatgatagt tgatattaaa ggtgcttttg gtggtttttg gctgttctga
      241 taatgataag cctttgatgg catttaatga aggcttttct tcaaacaaca ccgcaaggcg
      301 attaccataa tcaggtgcat aatcggtgtc ttgtttgtcg ttataatgcg tcaatgccag
      361 attgatattt tgcttgtcgt caagttgcca atcaacattt gtattgacac ttaggctttt
      421 gctgtcttgc ttatcagttt gggcaggctc aggagcgatg cgtttaccgt tagcatcaaa
      481 tgcccctcct gtggtgcgat agtcgacatc aagtcgtgca aggacattac cattttcgct
      541 gacacctgct acactctgac ctacctgata accgatacct tcactggata atttactacc
      601 atgtacaccg atgcgggttt caaattgctc ctcttccaaa tcagacttag taacgatatt
      661 aatcaaaccg cctgttgctc cagacccata aatactggtt gctcctgata aaacttcaat
      721 tctagcaact tgattgggat tgatactatt aagctgtcta gagatgtctc gcgaacctgt
      781 caaaggcacg ccatttaaca aaaattgcac ttgacgaccg tgcatggttt gcccaaagtt
      841 actggtagtg ccactactta cccccaaact tggaatgagc tgtgccatca catcggcaag
      901 ctgtcgagag cctgttgctt gctcttgaat ttgctcttca taaataactt tggtggtatg
      961 gggcatttgc gtgatacgat tggcaagtgc cataccgctc ttatcaatgg taataaccac
     1021 aggctctaat tcaaccgttg gtaatgattc attgggttga gcgtacaact gctgagttac
     1081 tgccacactt agtaaaggca aagataacca ttgaaaatag tgggaatgac gcataaatta
     1141 ctccttatgg aaaagtaaaa ttttaacagt ctgttacggt ttaacagtct gttacggaaa
     1201 atttttttat caatgtcggc aaaataagca ttgacacccc agaaccaatc gcaagccaca
     1261 aaaatagctt gtcatagcca aactgatgta ctaaaaatcc actcatcgcc ccgccgataa
     1321 aatacacaag caactccacg cacacaaaca aagtaaagtc cgtgccagct tggtcgggat
     1381 tggcagcatt cataaacaga gcatataagg cagtcattgt taaatagcga acagataaaa
     1441 ttagcaaaac actaattgcc atcaagctca taaaccctgt ttttaataaa cctaccgcag
     1501 gtaaaaaatc acccaataaa ggatatagca atcctagaca caacaaagca taaactgccg
     1561 ttcttagcca tccgaataat ataagacaac gtacaatgtc gatatatttg attaataccc
     1621 ctgccaaaag tgctgaacca atcccaacaa tcgctccaaa taccgaaaac aaaagcccaa
     1681 tctgagacag tgaaaaacca aaatccacaa gcattggatt tagcattgcc atcatcggag
     1741 cttccacaaa gcgaaacacc acaatcaaag ctagtaacca tttagtttgc gggcgtttga
     1801 taagcctaat aagacttgct ctggaaagat tggtagcatg tttgggattg tcttgatctt
     1861 ttaagaacca tagtgaacac gaaataagca caacagttgt tgccaaactg attgtcgcca
     1921 tttgccaatc aaatctctga tatagccaca gtgtgccagc accgcctacc atactaccaa
     1981 acgccactcc aaaactttgc atggcacttg ccagcccaaa ggatgactta tcaaattgtt
     2041 ccactgtata gccatcaata gcgatatctt gggtggcact tgccaaagac accaaaaaac
     2101 ttatgctcag aaataataca atctcagact cagaaaactg taaaaagcca agcccaaaca
     2161 gcccgactac cacgataatt tgagtaaaaa ataaccaagt tttgcgtttg ccgattttgg
     2221 cacaataata attgtccaca taacttgccc acagaaattt aaacgcccac ggcaaatata
     2281 aaagtgaaag catgccaatc accctaatat caacgccttg atgtcttaaa atggcaggca
     2341 atgccacatt aaaaaaatac aaaggcaagg catgggcaac atataacagt gccaccatcg
     2401 tcaaaagatg gcgatttttt tgttgtgaga taatcatcag accgccccta ttttagtgtt
     2461 tatccatgct ttggcttcag tcagtgacaa caccaccgca taaggcactc gccatgcttt
     2521 atgaaaagca ggactgtcta aggcttgtct ttgagtctca tcatcaacaa tt
//
LOCUS       DAWFBO010000191         2535 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFBO010000191 DAWFBO010000000
VERSION     DAWFBO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.191"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(227..1861)
                     /gene="groL"
                     /locus_tag="WGO87_001887"
     CDS             complement(227..1861)
                     /gene="groL"
                     /locus_tag="WGO87_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0393200.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM"
     gene            complement(1954..2244)
                     /gene="groES"
                     /locus_tag="WGO87_001888"
     CDS             complement(1954..2244)
                     /gene="groES"
                     /locus_tag="WGO87_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0393201.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGSRRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            2455..>2535
                     /locus_tag="WGO87_001889"
     CDS             2455..>2535
                     /locus_tag="WGO87_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0393202.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAPFFCT"
BASE COUNT          527 a          661 c          658 g          689 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaataaca atgcaaagaa
      121 cggtgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaccgcacg
      181 gcgttgaccg tgcggcttgt tgtctgaagg cttcagacgg cattgcttac atcatgccgc
      241 ccataccacc catgccgccc atatcaggca cagccggttt gtcttcgggg atttcagcga
      301 tcatgcaatc agtggtcagc atcaagccgg cgatagatgc ggcgtgttgc agcgcggaac
      361 gggttacttt ggcggggtcg agtacgccca tttcgatcat gtcgccgtat tcgccgctgc
      421 cagcgttgta accatagttg cctttgcctt ccaacacttt gttcacaacc acgctgggtt
      481 cgccgcctgc gttggcaacg atttggcgca gcggagactc aacggcgcgc aagacgattt
      541 gtacgcctgc gtcttggtcg gcattgccgg tgtgcaggtt ttccaaagca gcacgggcac
      601 gcaacagggc tacgccgccg cctgcaacca cgccttcttc aacggctgcg cgggtagcgt
      661 gcagcgcgtc ttccacgcgg tcttttttct ctttcatttc gacttcggtc gcggcaccga
      721 ctttgatgac tgccacgccg cctgccaatt tagccacgcg ctcttgcagt ttttctttgt
      781 cgtaatcgct ggttgcggtt tcgatttgtt ggcggatttc ggcaacacgc gcttcgattt
      841 gggctgcgtc gccaaagccg tcgatgatgg tggtgttttc tttaccgatt tcgatgcgtt
      901 tggcttgacc caagtcgtcc aaagtcgctt tttccaaaga cagaccgact tcttcggaaa
      961 tcaccacgcc gccggtcagg atggcgatgt cttgcaacat cgctttgcgg cggtcgccga
     1021 agcccggggc tttgacggca acggttttca ggatgcctcg gatgttgttc acgaccaaag
     1081 tcgccaaggc ttcgccttct acgtcttcag cgataatcaa cagcggacgg ctggctttcg
     1141 ccacttgttc caaaacaggc agcaagtcgc ggatgttgct gatttttttg tcgaacagca
     1201 atacaaacgg attgtccaaa gcagcgattt gtttttccgc gtcgttgatg aagtaaggag
     1261 acaggtagcc gcggtcgaac tgcatacctt caaccacatc cagctcgttt tccaaagatt
     1321 tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct tcggcgataa
     1381 tcgcgccgac ttgttcgtca gagttggcgg aaatcgagcc gacttgggcg atttctttag
     1441 aagtgtcgca aggtttggcg atgtttttca gctcgtcaac caaagcggca acggctttgt
     1501 cgataccgcg tttcaggtcg gtcgggttca taccggcggt aacgtatttc ataccttcgg
     1561 caacgatgga ttgcgccaat acggtggcgg tagtcgtacc gtcgcccgcc acgtcgttgg
     1621 ttttggacgc gacttctttc accatttgcg cgcccatatt ttcaaacttg tctttcagtt
     1681 cgatttcttt ggcgacggtt acgccgtctt tagtgatgtg cgggccgccg aatgcacggt
     1741 caacgactac gttgcgacct ttggggccca aggttacgcg gacggcgttt gccagaatgt
     1801 tcacgccgtt taccattttt tgacggactt cattgccgaa ttgtacgtct tttgctgcca
     1861 tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc gtctgaaggc
     1921 ggtttgccgt ttcagacggc atcgtgtccg tatttatttt tcaacgatgc cgaaaatatc
     1981 ctcttcgcgc atcaccaaca gctcttcgcc gtcggctttt acggtttgac cgctgtattt
     2041 gccgaagatg attttgtcgc cgactttaac atccagcgga cggcggctgc cgtctttgcc
     2101 gattttgccc gcacccacgg cgatgacttc gcccatatcg ggtttttcgg cggccgcacc
     2161 cggcaaaacg atgcccgatg cggttttttc ttcagcttcc aagcgtttga cgacgacgcg
     2221 gtcgtgtaaa ggacggatgg tcatatttat gctccgataa atggtttgaa aacaatcatc
     2281 tgcccgaacg gttcggacag attgaagtgg aaaccggaca gccgtcaggc ggctgcccgt
     2341 ataagtcggc aaaattaggg tgtgtgcggg caaattcaag tgaggcggaa aaaatttatt
     2401 tccgccgttt tttatagtgg actaaattta aggggctgta ctagattagc agatatgtta
     2461 ccctcgaaat atgaagataa cgcactgcaa attaaagaaa aaagtacaga aagaactgct
     2521 ccgttttttt gtact
//
LOCUS       DAWFBO010000192         2523 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFBO010000192 DAWFBO010000000
VERSION     DAWFBO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.192"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(31..837)
                     /locus_tag="WGO87_001890"
     CDS             complement(31..837)
                     /locus_tag="WGO87_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0393203.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1057..2427
                     /gene="ffh"
                     /locus_tag="WGO87_001891"
     CDS             1057..2427
                     /gene="ffh"
                     /locus_tag="WGO87_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0393204.1"
                     /translation="MLDNLTSRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEITTAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          701 a          625 c          652 g          545 t
ORIGIN      
        1 ggggcttcag acggcatctg ttggctcttc ttatcttttc agaatgattt ccaatacgaa
       61 cttgctgccc atataggcaa tcataaggct gacaaatccg atgatggtcc acacggcggc
      121 ttttttgccg cgccatgcgg tcatgctgtg cttgagcagc agtccgccgt aaatcagcca
      181 tgacaatatg ccgaatacgg ttttatgggt aaaggtcatg ggtttgccga atacggcttc
      241 ggcgaagaat gtgccgctga cgacggaata ggtcagcagg atgaaacctg cccacatggc
      301 ctggaacatg agtttttcca aactgagcaa cgacggcagg aatcctgcga gcttggagaa
      361 gctcctgcgg tgcaggctcc gattcagcag cagggtcaaa acggacaata atgttgcgat
      421 gccgaacagc ccgtatgcga gcagcgaagt tccgatatgc agcataaagg gaaggtcggt
      481 aatttcatat cccgagaatt ttccaggaaa aaccaaacct gacagcagca tcagtgcggc
      541 gcaaggatac agcagcaact gcactccgcg cagcggataa aaaaagctgc cggcaaaata
      601 aataaacagc atcatccaaa caatcaggct gccggaatac ccgaagccca taatgatgat
      661 tttgtcttga atgaccggca taagcagtgc cgcgccgtgg acggtcaatg ccgcgcccaa
      721 aaccggcaat tccgtcttcc acgggtaatc ccggccgcgc ccctgctgtt ggcagtgcca
      781 tgcaaatgca cccaatcctg cgtaaaccgc cgtcaaaaag atgaaaactg tcggcatggt
      841 ggactttctc tatctatact gttgcgccgt atgcggtcgc ttatgaaata ttggaacttt
      901 taacgttgga attgtaaaat ccccgtttcg ggcaagcctt gacggatttg ccgatatgct
      961 gtccggcaca caagccgcat caaattattt tgattttatt ttaacaaaga atgcccctga
     1021 tggggcaagc tattcttatt cagaccaagg accagtatgt tagacaattt aaccagccgc
     1081 ttcagcaatg tcttcaaaaa catccggggg caggccaaac tgaccgaaga caatattaaa
     1141 gaagccttgc gcgaagtccg cctcgccctg cttgaggcgg atgttgccct gcctgtcgtc
     1201 aaagagttcg tcaacaacgt caaagaaaag gccctcggtc aggaagtggc gggcagcctg
     1261 acgcctgacc aagcctttat cggcgtggtc aaccaagcct tagtcgaact gatgggcaag
     1321 gaaaacaaaa cgctggattt gtcggtttca ccgcccgccg tcgtgttgat ggcgggtttg
     1381 cagggtgccg gtaagacaac aaccgtcggc aaactcgccc gcctgttgaa aaacgatcag
     1441 aagaaaaagg ttttggtggt atccgccgac gtttaccgtc ctgccgcgat tgaacaactg
     1501 cgtctgttgg ccgaacaggt cggcgtggac ttttttccat ccgataccaa ccaaaaaccg
     1561 gttgagatta caactgccgc cgtcgattac gccaaaaaac atttttacga tgtcttgatg
     1621 gtcgataccg ccggtcgttt ggcaatcgat gaagagatga tgaacgaaat caaagcgctt
     1681 catgcggcgg tcaatccggt tgaaaccttg ttcgttattg atgccatgtt gggtcaggat
     1741 gcggtgaaca ctgctcaggc atttaatgaa gctctgccgc tgaccggtgt cgtattgacc
     1801 aagatggacg gcgactcgcg cggcggtgcg gcattgtccg tacgccatgt aaccggcaaa
     1861 ccgattaaat ttatcggcgt cggcgaaaaa atcaacggcc tcgaaccttt ccaccccgac
     1921 cgtcttgcca gccgcatcct cggcatgggc gacgtattga ccctgattga agacgttcaa
     1981 aaaggtattg atgaagaagc cgccgccaaa atggcgaaaa agctacaaaa aggcaaaggc
     2041 ttcgacctca atgactttaa agaacaaatc cagcagatgc gcaatatggg cggcttggaa
     2101 aacctgatgt cgaaaatgcc gggcgaactg ggtcaaatat cgaaacaaat ccccgaagga
     2161 acggctgaaa aagcgatggg caaagtggaa gccatcatca actcgatgac tccaaaagaa
     2221 cgcgccaacc ctgccctgct caaagccagc cgcaaacgcc gtattgcaat gggtgcgggc
     2281 acgaccgtac aggaagtgaa caaattgctc aaacagtttg aacaaatgca acaaatgatg
     2341 aagatgttca gcggcaacgg cttgggcaaa ctgatgcgta tggcgaaagg aatgaaggga
     2401 atgaaaggaa tgttcccggg tttgtaagcc gatttgaccg aaaacgccgt ctaaaatttc
     2461 agacggcgtt tttgttttat attctgattt atagtggatt aacaaaaatc aggacaaggc
     2521 ggc
//
LOCUS       DAWFBO010000193         2453 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFBO010000193 DAWFBO010000000
VERSION     DAWFBO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.193"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            148..597
                     /locus_tag="WGO87_001892"
     CDS             148..597
                     /locus_tag="WGO87_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0393205.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
     gene            complement(687..842)
                     /locus_tag="WGO87_001893"
     CDS             complement(687..842)
                     /locus_tag="WGO87_001893"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0393206.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(1139..1351)
                     /locus_tag="WGO87_001894"
     CDS             complement(1139..1351)
                     /locus_tag="WGO87_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393207.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            1583..2056
                     /locus_tag="WGO87_001895"
                     /pseudo
     CDS             1583..2056
                     /locus_tag="WGO87_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2171..2324
                     /locus_tag="WGO87_001896"
                     /pseudo
     CDS             2171..2324
                     /locus_tag="WGO87_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271703.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
BASE COUNT          686 a          478 c          594 g          695 t
ORIGIN      
        1 gtcggcgcgc ccgcaccaaa aggcccagcc aaaccggcta taccggccgc taaaaaatag
       61 agtgggaaat atgcatactg ttgaatggga tagtaagtct gttcaaagaa atatgttgaa
      121 taatctgttc ttattggaag taaagtaatg actgataatc gggggtttac gctggttgaa
      181 ttaatatcag tggtcttgat attgtctgta cttgctttaa ttgtttatcc gagctatcgc
      241 aattatgttg agaaagcaaa gataaatgca gtgcgggcag ccttgttaga aaatgcacat
      301 tttatggaaa agttttatct gcagaatggg agatttaaac aaacatctac caaatggcca
      361 agtttgccga ttaaagaggc agaaggcttt tgtatccgtt tgaatggaat cgcgcgcggg
      421 gccttagaca gtaaattcat gttgaaggcg gtagccatag ataaagataa aaatcctttt
      481 attattaaga tgaatgaaaa tctagtaacc tttatttgca agaagtccgc cagttcgtgt
      541 agtgacgggc tggattattt taaaggaaat gataaggact gcaagttact taagtaggct
      601 tgtttacaaa tcgatcgata gtgtttcagt atttgtgcgg ttttccgcct tgatggaatg
      661 aaaatcgtgt aatattaagc ctatagttat gactcgtaat ctgtatctgg attgtttctg
      721 gataaaagaa taaacccaat cgttgggtcg tttgggttta tttcattgat tgacagaagt
      781 tttttaataa atggaaagac aacgcctttt ttaggggatg gttttgtttt tctctctgat
      841 attgcctata ggtttctgcc ccttccattc taaatatatt atccgattcg gataagtcga
      901 atagggcact tgatgctaag ccgattacaa gtcgtttcga taaatcatag gacataataa
      961 ggatatcttt agccgtggat ttatggggta tagtagcaaa aattgtatag gggtgtacat
     1021 tactcatctg aaaaggtgaa cagattttat aatgctttca gacggcattt tgtttccaat
     1081 tcgttatagg cttgtatttt gcctatgtaa tatagtaatt taaggtatgt ttgcaccttt
     1141 agacataagg gcaatcgcag ctttcgtctt taatatacaa atcactatcc agcttcagcc
     1201 atacccaata gttttccaaa ccgcatacct ccgcccgata acgcccggtg gaagtttttt
     1261 ggacagcacc aactttgccc tgctgaaaat agagcagtcc gcgttcggcg atatggggcg
     1321 ggaacaggtg gagcgggtgg atatggggca tggtttgctt ccaagaagtt gttgaaatat
     1381 gagaagttta ctggttcggg cgatgtttgg ggagaggatg gtgggaagat ttgttttaca
     1441 aagtggcaaa aggtcgtctg aaaatagggt tcagacgacc ttttggttta gttgggaagg
     1501 gctgtggttt aaaaagactg agacctttgc aaaactcccc aaaatcccct aaattcccac
     1561 caagacattt aggagatttc tcatgagcac cttctttcag caaaccgccc aagccatgat
     1621 tgccaaacac atcgaccgtt tcccactatt gaagttggac caggtgattg attggcaacc
     1681 gatcgaacaa tacctgaacc gtcaaaaaac ccgttacctc cgagaccacc gcggtcgtcc
     1741 cgcctacccg ctgttgtcca tgttcaaagc cgttctgctc ggacaatggc acagcctctc
     1801 cgatcccgaa ctcgagcaca gcctcatcac ccgcatcgac ttcaacctgt tttgccgctt
     1861 tgacgaactg agcatccccg attacagcac cttatgccgt taccgcaact ggctggcgca
     1921 agacgacacc ctgtccgaat tgctcaaact gattaaccgc caactgaccg aaaaaaacct
     1981 aaaagtagag aaagcatccg ctgccgtcgt tgacgccacc attattcagg ccgccggcag
     2041 caaacagcgc caggcccgat aacgcccggc ggaagttttt cggacatcgc gaaccttgcc
     2101 ctgctgaaaa tacaaaaggc cgcgttcggc gatatggggt gggaacaggt gaggagggtg
     2161 gatacggggc atggttggct tccaagaagt tgttgaaata tggggagttt actggttcgg
     2221 gcgacgtttg gggagagggg tgggaattct tgttttgcga agtggaaaaa ggtcgtctga
     2281 aaatgggttt cagacgacct tttttagggt ggaaagggga gtagtttaga aagtccacac
     2341 atattctgcg tatggattcc aagttgtata gggtggtatc ttccaaaaat acatatgagt
     2401 aggatagtgt tatgagttga caacttgttt attgaaaata tctttcttga act
//
LOCUS       DAWFBO010000194         2451 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWFBO010000194 DAWFBO010000000
VERSION     DAWFBO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.194"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            25..555
                     /locus_tag="WGO87_001897"
     CDS             25..555
                     /locus_tag="WGO87_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0393208.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKW"
     gene            697..2358
                     /locus_tag="WGO87_001898"
     CDS             697..2358
                     /locus_tag="WGO87_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0393209.1"
                     /translation="MTESITRDNMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     VTPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVMHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIAYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMINVNYKEYASPETRYCPAGVYEIIE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT          621 a          601 c          678 g          551 t
ORIGIN      
        1 cctgttgctt atggaaaaat caaagtgcag agttggaaag agaggcgcga tttcaatatt
       61 gtaaagcagg atttggattt ttcctgcggg gcggcttcgg tggcgacgct tttgaacaat
      121 ttttacgggc aaaagctgac ggaagaggac gtgttaaaaa aactggataa ggagcagatg
      181 cccgcgtcgt ttgaggatat gcggcgcatt atgcctgatt tgggttttga agcgaaaggc
      241 tatgccctgt ctttcgagca gcttgcacag ttgcaaatcc ccgtcatcgt gtatctgaaa
      301 taccgcaagg atgatcattt ctcggtattg cgcggtatag acggcaatac ggttttgctt
      361 gccgacccgt cgctgggtca tgtttcgatg agcagggcgc agtttttgga tgcttggcaa
      421 acccgtgagg gaaatttggc aggtaagatt ttggcggttg tgccgaaagg gagggatgcc
      481 ggcggggata aggcgttttt cacacgcagt ccgaaacgtc agacggagtt tgcggtcgga
      541 caggtaaaat ggtagcgtgc ttattgaaat ttcgacaaag gtcgcctgaa aaccgaaaat
      601 atggttttca gacgaccttt gttgtatttg gtaactatat gttcccgttg tataattacg
      661 gatttgcaat tcaataataa atacacagga accgccatga cagaatccat cacccgcgac
      721 aatatgcaat acgatgtcgt gattgtcggc gcaggcccgt cgggtttgtc cgccgccatc
      781 aaactcaagc agcttgccga acaaaatggg cgcgaaatca gcgtttgcgt ggtggagaaa
      841 ggttcggagg cgggtgcaca ttcgctggcg ggcgcggtta tcgatccgat tgcgctgaat
      901 gagttgattc ccgactggaa agaaaaaggc gcgccgctga cacgcacagt aacgcaggac
      961 aaggtgttgt tcctgaccga gaaaaaagcg ttcaacctgc ccgttacccc caatttcgac
     1021 aatcatggca actacatcgt cagcttgggc gaagtcgtac gctggttggc ggagcaggcg
     1081 gaaaatttgg gcgtggaaat ttatccgggc tttgccgctg ccgaagtgct gtatcacaaa
     1141 gacggttcgg tcaaaggcat tgcaaccggc aatatgggcg tgggcaaaga cggcgaaccg
     1201 accgattcat tccagcccgg catggagctt tgggcacagc aaaccctgtt tgccgaaggt
     1261 tgccgaggtt cgctttccaa gcaaatcatc gaacgcttca aactcgacca aaacagccag
     1321 ccgcaaactt acggcttggg cattaaagaa gtttgggaag tgccgtccga acagcatcag
     1381 cccggtttgg tgatgcacag cgcaggctgg ccgctggaca gcaaaaccta cggcggcgcg
     1441 tttgtttacc atttcgacga caacaaagtt gccgtcggct tcgtggtcgg tttggactat
     1501 caaaatcctt atctgtcgcc gtttgaagag ttccaacgtt tcaaaaccca tcccgaaatc
     1561 cgcaaaacct ttgaaggcgg ccgccgcatt gcttacggcg cgcgttcgct gattgaaggc
     1621 ggtttgcaaa gtttgccgaa gctctcgttc aaaggcggca ttttggtcgg cgatgcggcg
     1681 ggtttcctca atatgccgcg catcaaaggc attcataccg cgatgaaatc cgccatgctc
     1741 gccgccgaag ccgtgttccc tttgttggaa aaccttgaag aagtggaggg tttcgacagc
     1801 ggcaaagagg cggcggatta tcagcaacgt tttgaacaaa gctggctgta tcaagagctt
     1861 tacgccgcgc gcaacgtccg tccgtcattc aaatggggcg tttacctcgg ctcgctttat
     1921 accggcatcg accagatgat tttcagaggc aaagccccgt ggaccttgaa acatcacggt
     1981 aaagacaacg agcagctcaa aaaagccgcc gcgtgcaagc cgattgctta tccgaaaccc
     2041 gatggcgtgt tgaccttcga ccgcttgagc agcgtcttcc ttgccaatct cgcgcacgaa
     2101 gaaaaccagc ccgaccattt ggtgctgaac aatccgcaaa cgatgataaa cgtgaactac
     2161 aaagaatacg cctcgcccga aacgcgctat tgtccggcgg gtgtgtacga aattattgaa
     2221 gaaaacggca atccgcgcct gcaaatcaac gcggcaaact gcgtgcactg caaaacctgc
     2281 gacatcaaag acccgacgca aaacatcact tggatttgcc ccgaaggcgc gagcgggccg
     2341 aattacggcg ggatgtaaac cttattccgc cgttacgccc ggtcaggaat aaggaatggg
     2401 gcagttgttc aaacctgccc cgtttgaacc cataatatag tggattaaac t
//
LOCUS       DAWFBO010000195         2450 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFBO010000195 DAWFBO010000000
VERSION     DAWFBO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.195"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(136..1269)
                     /locus_tag="WGO87_001899"
     CDS             complement(136..1269)
                     /locus_tag="WGO87_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393210.1"
                     /translation="MFKKFKLVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(1443..1892)
                     /locus_tag="WGO87_001900"
     CDS             complement(1443..1892)
                     /locus_tag="WGO87_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393211.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENEIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCNISHFLHFADRA
                     EADIPNQSRSYAEYWWEMEMFKMSVLEDWERSGRPLNWEKWQRQYRS"
     gene            complement(2058..2381)
                     /locus_tag="WGO87_001901"
     CDS             complement(2058..2381)
                     /locus_tag="WGO87_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393212.1"
                     /translation="MNILASDRNYELNIEIFIDDIIHFQITDESYKVKFSEYLFENKT
                     ENNSDGNPTANYFFEIIDDSYMDWLKEESFGFFEKKYYKAYIFFFSDSVIEVISSTEP
                     VFYSK"
BASE COUNT          667 a          602 c          427 g          754 t
ORIGIN      
        1 ccgtttaatg tgtatttccg ttttttggat tgtggttttc aatttgtagc gaatcggatt
       61 cggcatatac ggcattgcaa aaagcgtttg actctccaat gccgtctgaa aaccggtttc
      121 agacggcatt tgcgttcagt gagaaaggtc gcgcctgccg cccgaacgtc tcgccgcagc
      181 ctctgcataa cggctcacct ctttttccaa attttccaag ttcaaaggaa aatcaggcag
      241 tttgtctccc tgtttctctt cgcggataat ccgcccgcca tccaaatacc acgtctgttg
      301 cgcatgatag gtctgtatat ccgccgttac gccatccgct ttcaatgcta ccgtcgaaga
      361 ttgtgcaata aaaagatttc cgtttttcaa ataatattcg aaactctggc gtttttttcc
      421 attgtcgaaa ctccaataga ctttttgcgg cagaccgtcc gcatcatagc cgaccacaag
      481 actgttcgcc ttcatccccc ggggcatcaa ttcccgcata ttctgataaa acacagaatc
      541 gcgcgagtcc gatgcaatcc ggttgctctc ttcgcggaag tcccaaacct tctgctcatc
      601 attcgcgaca tcccggtatt tcgccaaata tacctgggcc atctgataac acccgaggca
      661 atgctcataa acatcttccc cgattttccc gcgccccgac gcatcaaata ccgaaccgtc
      721 cggttgccaa acaacccgat attctcctgt cgtttcataa ttttctccgt gagccgttcc
      781 gccgtacaca tttacagaaa acggacgatc gttccgatac agatattcgg cattaacaaa
      841 cgcttccggc gagcgttgcg aaagcgaaac cgcaaccaaa ccgccctcgc cgatatggta
      901 atccagccaa acctctttcc catgttcctg ctccgttacg tgaaaccatt tcgccttttc
      961 tttcaaacga ctgagccgga tagcgagcgc gagataatcc ttctccgact gcaacggacc
     1021 gtcatccaca gctccggcaa gactttcctc cgtcctcatc gattccttca cgatgacaac
     1081 cgccctgtcg gcatttcgga acaggcgggc aagtttcgcc acaaaagcat tcggattttc
     1141 aggtatttca gttgccgtat cgctcaaaaa ccaacgcgga ttaatctcat aggcaatacc
     1201 cgtccccagc caaaaggcaa atacaagggc aaaaaatgac aacaatacca gtttgaattt
     1261 tttaaacata tttatttttc gtttaattcg tttaacagga tatatcgatt atatcagacg
     1321 agctttgatt gtttgatttt taatctaatc tacgcttgct gccgtacttt aaaaaaacct
     1381 atgcaggctg cggcttgtta ctcttgtttg ctttgattca aattttcaac taattcaaat
     1441 gcttaagatc gatattgcct ttgccatttt tcccaattta acggtcttcc cgagcgttcc
     1501 caatcttcta aaaccgacat tttgaacatt tccatttccc accaatattc ggcatatgaa
     1561 cgtgattgat tgggaatgtc ggcttctgcc cgatcggcaa aatgcaaaaa atgagaaata
     1621 ttgcaaacta tattattttc aggaacttct tcggcaaata gatgcttgac tgactgtaac
     1681 gcttcggtct gatatttttc tgtccatttc tcccattttt caggctttcc cgatatttcc
     1741 caatcttcca atgcggaagc attcaagatt tccaattccc accaacagtc gtaataacgg
     1801 gaggattggt taggaatctc attttctgct tcggaaacaa attgggtaaa actaaaaata
     1861 tccttaacga ttttttcaaa tctattttcc ataggttgcc gcccctccca attgcgtgcc
     1921 aacaatttcg gcaaaatcaa gccggcaagc cccggcatcc tgtgctaatc ggagaaaatg
     1981 ccgtctgaaa gccgaatacc gcttcagacg gcattttgat tgttgggttt ttaatcagag
     2041 ctttgtttga taatttgtta ttttgaataa aatacaggtt ctgtcgagct gataacttct
     2101 attacagaat cgctaaaaaa gaaaatgtaa gccttataat atttcttttc aaaaaaacca
     2161 aaactttctt ctttcaacca gtccatataa ctgtcatcta ttatctcgaa aaaataattt
     2221 gcagtaggat ttccatccga attattttct gttttatttt cgaataaata ttctgaaaat
     2281 tttactttat aagattcatc tgttatttga aaatgaatta tatcgtcaat aaatatttca
     2341 atatttaatt cataatttcg gtcggatgcc aaaatgttca catcttgttc ggtaacacaa
     2401 attttgtcca cataaaatct gtgattattg ttagaaaatt gtaatggttt
//
LOCUS       DAWFBO010000196         2374 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFBO010000196 DAWFBO010000000
VERSION     DAWFBO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.196"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..1071
                     /locus_tag="WGO87_001902"
     CDS             <1..1071
                     /locus_tag="WGO87_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0393213.1"
                     /translation="TFFDKYGVRVSLLEDEDFIRAASMLAEEVFGFGIYKESKGSGGR
                     FYERCWLMGSEDVLYGRVHFGGQNNTILFELTGTGCGVAKEGWESRLFAFLTNAIRPK
                     ITRVDIAKDFFNGEYSPNQAREDRNKGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKT
                     YGIGSRESSKYVRVYEKGKQLGDKTSTWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAY
                     PICERFVQKATRIHAVKEDKVISADRYLEWVKKQFGRAANGLKFIFPELDKAKLFELI
                     EPSHHKLPKSLAPEAYDCAFLKAQAIHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHV
                     NNEESYNNFIYDKFARLPISWA"
     gene            1116..1406
                     /locus_tag="WGO87_001903"
     CDS             1116..1406
                     /locus_tag="WGO87_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393214.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGRSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1411..1608
                     /locus_tag="WGO87_001904"
     CDS             1411..1608
                     /locus_tag="WGO87_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393215.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            1616..1900
                     /locus_tag="WGO87_001905"
     CDS             1616..1900
                     /locus_tag="WGO87_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393216.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            1907..2185
                     /locus_tag="WGO87_001906"
     CDS             1907..2185
                     /locus_tag="WGO87_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393217.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT          709 a          542 c          542 g          581 t
ORIGIN      
        1 acctttttcg ataaatacgg cgttcgtgta agtcttttgg aagacgaaga tttcattcgt
       61 gccgcgtcca tgctcgccga agaagttttc ggtttcggta tctacaaaga atccaaaggt
      121 tcgggcggtc gtttctatga gcgttgttgg ttgatgggtt cggaagacgt cctatacggt
      181 cgcgtccatt ttggcggaca aaacaatacc atccttttcg aactgaccgg caccggttgc
      241 ggcgtcgcaa aagaaggctg ggaatcacga cttttcgcat tcctgactaa tgcaatccgc
      301 ccaaaaatca cacgcgttga catcgcaaaa gactttttca acggcgaata cagcccgaac
      361 caagcccgtg aagaccgaaa taaaggtatg tttacctgtc atcacgtcaa accaaaaggc
      421 gaatgtttgg ggtcagattg ggaagaagac gatgaagcca aaatgaccaa aggcaagacc
      481 tatggtatcg gctcccgtga atcgtccaaa tatgtccgcg tctatgaaaa aggcaagcag
      541 ttgggcgata aaacaagcac atggacgcgc tttgaaattg aattcaaagc aaaagacatc
      601 gttatccctt ttgaagtttt gcagaatccg ggcgaatatt tcggcggcgc atatccgatt
      661 tgcgaacgat tcgtccaaaa ggcaacgcgc atacacgcgg ttaaggaaga taaggtcatt
      721 tcagccgacc gctaccttga atgggtaaaa aaacagtttg gacgtgcggc aaacggtctg
      781 aaattcattt ttcccgaatt ggacaaagcc aaactgtttg aactgattga gccgagtcat
      841 cacaagctgc ccaagtcttt ggctcccgaa gcctacgact gcgccttttt gaaagctcaa
      901 gccattcatg aacagcccgc attcaaaccg tacaaagacc cttactatat gtacgaatat
      961 tacgagaatc ttgaaaaaca gcttgaacag caaaaacacg tcaacaatga agaaagctat
     1021 aacaacttca tttacgacaa attcgcaaga ctaccgattt catgggctta aagtgtctgt
     1081 ccgaaagacg tttaatcaca caaggaaacc aaaaaatgaa catccaactt caaggccaca
     1141 tcgtcggcgt taaaaaattc aacggacaaa tcgaaggcag gagcttcgac tattgccgcc
     1201 tgattgtcgc cacaccctta gacagctccc aaggcaacgc attgggcagc tctactactg
     1261 aatacgattt cggcggctct gccaatttcg agcagttccg aaacgcccaa tttccgatcg
     1321 aagcaaacct gaacgtagaa atcgtcacta cgggcaaaac ccaaaaactg aaagtcatcg
     1381 gttttcaact cgtgaagaaa ggctgattga atgcagaaag tctatgttgt ccagtccgta
     1441 tcaacagggg actttctgta tctctctcct gaaacgggcg acatcggaca taccaaatta
     1501 atcaccaatg ccgattattt ctacgacttc gaagaagcga ttaacgcagg tttggaagaa
     1561 atcggcaacc aatacgaatt tgtcgtattc ggatttttga aagactgatt ttcggatgtt
     1621 cggcggtcgt ttgaaaaacg ctccatccat taccgccaaa cactttttga aggaaaatat
     1681 catgaaattt attaacacct gccgtaaata cggcgcaaaa ctggctgttg taacagctgc
     1741 tcccctggct ttggccgcac atgcaaatgc aacgttgccc gatacggcaa aaaacgcttt
     1801 ggaagccgca aaagcggacg gtatggaagc cggttggatt gtcgttggcg ttttcgccgc
     1861 gctttttgta ttttccatcg ttaagagagt gatgaagtaa gacggcatgt actaccaagt
     1921 cggaaataaa tgtcttgaga agcaccaggc tgaaaacctt tatttcagct tggtagtacc
     1981 aagaatcaaa gaaaacggac agattgtcag gccggaatat aacggcagcc tgtggaagat
     2041 gtcggacggt cagccgctaa ggcttttatt ggcggaatgc agtctgaaag acaacctgca
     2101 aagcggcctt gaaacaggct ggatagtatt cggcatcctc gcgtccgttt actttgtttc
     2161 cctgctgaaa aaggttttga aatgatggat ttttattttt atctcggcgt ttccgtaccc
     2221 gtattaatcg gggcggttct gtttaagaat tgagcgcatg aagttatggt gtcaaaatca
     2281 ggctttcaaa acaatcattg aaaggcagaa ccatgaacaa gccgtttatc actcaggcgc
     2341 agttggcact ttataaatat cagccgtcta gcaa
//
LOCUS       DAWFBO010000197         2351 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFBO010000197 DAWFBO010000000
VERSION     DAWFBO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.197"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            271..2151
                     /locus_tag="WGO87_001907"
     CDS             271..2151
                     /locus_tag="WGO87_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ0393218.1"
                     /translation="MNIFYEESGQFKVAAVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKHGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGIKRVGIHIAAPS
                     LAIKQGGKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPAVSIYFDVDSK
                     FNVGEPTCKIEAVNIAENLRIQTIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          635 a          705 c          556 g          455 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagccgcgcc aaccctcccc
       61 aaaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgtt tcaacacaca ggacggcaca taaagcaccg ccctatgtgt
      181 tgccctgatt tggaaggggt tgcgcccctc ccaaataaat tctgattcta ccgccctaaa
      241 ggttggggtt tcaaccgaaa aggaaacacg atgaatatct tttacgaaga gtccggccaa
      301 ttcaaagtcg ccgccgtcgt ccaaaaaaac gatgccacct accaagtcga tacccaacac
      361 ggcaaacgca ccaaagtgaa ggcaaacaat gtctttgccg agtttgacgg cgatatggcg
      421 gcgtttttgg aaaacgcgca ggcgcaggcg gcggacatcg acaccgattt attgtgggaa
      481 gtatgcggcg aagaggaatt taccgccgaa gccatcgccg aagaatatta cggtcatgcg
      541 ccgaccaaaa ccgagctggc ggcaactttg attgcacttt acgccgcgcc ggtgtatttc
      601 tacaaaaaag ccaaaggcgt gttcaaagcc gcgcccgaag aaaccttaaa acaagcgctt
      661 gccgccatcg aacgcaaaaa acagcaggac gcacaaatcg acgcttgggc agaagccttg
      721 aaacacggcg agatgccgtc tgaaatcgcg gcggatttga aaaccatcct acacgcgccc
      781 gacaagcagt cgctgaccta caaagccttt accaaagccg ccgacgcgct gaaaacctcc
      841 gcctacgaac tggcgaaaaa aacgggcggc attacgtcca ttccgcaata cctgcaagac
      901 gggtttgaaa tcaaatactt tcctaaaggc acaggcttcc ccgacctccc ccttcccgaa
      961 atgcccgacc tgcccaaggc tgatgttacc gccttttcca ttgacgacga atcgaccacc
     1021 gaagtggacg acgctttgag cctgaccgac ttgggcaacg gcataaagcg cgtcggtatc
     1081 catatcgccg cgccgtcgct tgccattaaa caaggcggca aaatggaaaa aaacatcatg
     1141 gaacgcctga gtacggtcta tttccccggc ggaaaaatta ccatgctacc cgaaaactgg
     1201 attgccgcgt tcagccttga tgcaggtgca taccgccctg ccgtcagcat ttatttcgat
     1261 gtggacagca aattcaacgt cggcgagccg acctgtaaaa tcgaagcggt caacatcgcc
     1321 gaaaacctgc gcattcaaac catcgagccg catttcaacg ccgaagcggg cttggacgaa
     1381 gcgggcgaaa tgatgttcgc ccaccatcaa gacctgattt ggttttatca attcgccatc
     1441 accctgcaaa aagcgcgcgg caaatacgaa cccgaccgcg cgccgcaata cgattacagc
     1501 atcgaattgg atgaggaagg caaagtctcc gtcgtccgcc gcgaacgcgg ttcgcccatc
     1561 gatacgctgg tcagcgagat gatgattctt gccaacagca cttgggcaca aatgctccat
     1621 gacaacgacc tgcccggcct cttccgcgtc caacccaccg gaaaagtacg catgagcacc
     1681 aaatccgagc cgcacatcgg catgggcgtg cagcattacg gctggttcac ttcgccgctg
     1741 cgccgcgccg ccgactacat caaccaaaag cagctgatca gcctgataga cgacactgcc
     1801 gagccgctgt atcaaaacag cgatgccgag cttttcgccg cgctgcgcga ctttgatgcc
     1861 gcctataccg cctacgccga cttccaacgc caaatggaag cctactggag cctggtttac
     1921 ctgcaacagc aaggcatcag cgagctgacg gcgacgattc tgaaagaaga cctcgtccgc
     1981 atcgaaggcc tgccgctggt cacgcgcgcg accggtattc cgtttgacgc actgcccaaa
     2041 tcgcaggcat tgttcaaaat caccgaatta gatgccgaga agcagtttgt ctcgctcaac
     2101 tacatcaagg cagtcgcacc cgcgggtaca acggcaggca atgccgtctg aagcccgata
     2161 cggcaaactc cggcaaaaac agaaacctga atctcatcat tcccacagtc gggaatccgg
     2221 ttttttgggt ttccgttgtt tttccgtttc aatgaacttc caagccgccg ttcccgataa
     2281 atacccgcaa tctaaaatcc cgtcattccc acgcagatgg gaatctaggt ttatccgcac
     2341 agaaactgat a
//
LOCUS       DAWFBO010000198         2338 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWFBO010000198 DAWFBO010000000
VERSION     DAWFBO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.198"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            43..2256
                     /locus_tag="WGO87_001908"
     CDS             43..2256
                     /locus_tag="WGO87_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0393219.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          598 a          755 c          566 g          419 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac gaaagaccca tcatgaccgc catcagcccg
       61 attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg aatggttcga cagctactgc
      121 gccgcgctgc cggacaacga taaaaaactt gtcttagccg cccgttcgct ggcggaagca
      181 cattaccccg ccgatgccgt cacgccgtat ggcgagccgt tgtccgacca cttcctcggc
      241 gcggcacaaa tggtcgacga actcgacctg ctgcccgatg ccgtcgccgc caccctgctt
      301 gccgacatcg gacgctacgt ccccgattgg aatctattgg tttccgaacg ctgcaacagc
      361 accgtcgccg agctggtcaa aggtgtggac gaagtgcaga aactcaccca cttcgcccgg
      421 gtggacagcc tcgccacgcc agaagaacgc gcccagcagg cagaaactat gcggaaaatg
      481 ctgctggcga tggttaccga catccgcgtc gtgttaatca aactggcgat gcgtacgcgc
      541 accctgcaat tcttaagcaa cgttcccgac aatcctgaaa agcgcgccgt tgctaaagaa
      601 accctcgaca tttttgcccc actcgccaac cgcttgggcg tgtggcaact caaatggcag
      661 ctcgaagatt tgggcttccg tcatcaagag ccggaaaaat accgcgaaat cgccctgctt
      721 ttggacgaaa aacgcaccga gcgcctcgaa tacatcgaaa acttcctcaa tatcctgcgt
      781 acggaactca aaaaatacaa tatccacttt gaagtcgccg gccgcccgaa acacatctac
      841 tccatttaca aaaaaatggt gaagaaaaaa ctcagcttcg acggcctctt tgacatccgc
      901 gccgtgcgga ttctggttga taccgtcccc gagtgttaca ccacgctggg cattgtccac
      961 agcctttggc agcctatccc cggcgagttc gacgactaca tcgccaaccc gaaaggcaac
     1021 ggctataaaa gtttgcacac cgtcatcgtc ggtcccgaag acaaaggtgt ggaagtgcaa
     1081 atccgcacct tcgatatgca ccaattcaac gaattcggtg tcgccgccca ctggcgttac
     1141 aaagaaggcg gcaaaggcga ttccgcctac gagcaaaaaa tcgcctggtt gcgccaactt
     1201 ttggactggc gcgaaaatat ggcggaaagc ggcaaggaag acctcgccgc cgccttcaaa
     1261 accgagcttt tcaacgacac gatttatgtc ctgaccccgc acggcaaagt cctctccctg
     1321 cccacgggcg cgacccccat cgacttcgcc tacgccctgc acagcagcat cggcgaccgt
     1381 tgccgcggtg cgaaagtcga agggcagatt gtgccgctgt ccaccccgct cgaaaacgga
     1441 cagcgcgtcg aaatcattac cgccaaagaa gggcatcctt ccgtcaactg gctttacgaa
     1501 ggctgggtca aatccaacaa ggcaatcggc aaaatccgcg cctacatccg ccagcaaaac
     1561 gccgacaccg tgcgcgaaga aggccgcgtc caactcgaca aacagcttgc caaactcacg
     1621 cccaaaccca acctgcaaga gcttgccgaa aatctcggct acaaaaagcc agaagacctc
     1681 tacaccgccg tcggacaagg cgaaatttcc aaccgcgcca tccaaaaagc ctgcggcacg
     1741 ctgaacgaac cgccgcccgt acccgtcagc gaaaccacca tcgtcaaaca gtccaaaatc
     1801 aaaaaaggcg gcaaaaacgg cgtgctcatc gacggcgaag acggtctgat gaccacgctt
     1861 gccaaatgct gcaaacccgc gccgcccgac gacattgtcg gcttcgttac ccgcgatcgc
     1921 ggcatttcgg tacaccgcaa aacctgcccc tctttccgac acctcgccga acacgcgccc
     1981 gaaaaagtac tggacgcaag ttgggcggcg ttgcaggaag gacaagtgtt cgccgtcgat
     2041 atcgaaatcc gcgcccaaga ccgctccggg cttttgcgcg acgtatccga cgcgctcgcc
     2101 cgccacaaac tcaacgttac cgccgtgcaa acccagtccc gcgacttgga agccagtatg
     2161 cggttcacgc tcgaagtcaa acaggtcaac gacctcccgc gcgtcctcgc cagcctcggc
     2221 gacgtcaaag gcgtattgag cgttacccgg ctttaaatac aaaaatgccg tctgaaagcc
     2281 gtataacgct tcagacggca ttttgattga cggggtttgc tattttttgt tgcatagt
//
LOCUS       DAWFBO010000199         2324 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWFBO010000199 DAWFBO010000000
VERSION     DAWFBO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.199"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(46..633)
                     /locus_tag="WGO87_001909"
     CDS             complement(46..633)
                     /locus_tag="WGO87_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0393220.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSDGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            796..2202
                     /gene="dnaB"
                     /locus_tag="WGO87_001910"
     CDS             796..2202
                     /gene="dnaB"
                     /locus_tag="WGO87_001910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0393221.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          570 a          601 c          620 g          533 t
ORIGIN      
        1 ataccgcaaa aatctacccg aaatgatata gcggattaac aaaaatcagg acaaggcggc
       61 aaaacgtttg gcaacttcgt cccagttgac gatttcccag aaacctttca ggtagttggg
      121 acggctgttg cggtagtcga tgtaataggc gtgttcccac acgtcgcagg tcagcagcgg
      181 cgtgttttca gtggtcagcg gcgtagcggc gttggaagtg gaaaccaaat ccaatccgcc
      241 ggcaggggtt tttaccagcc acgcccaacc ggagccgaaa gtaccggccg cgcaggcatt
      301 gaacgcttct tggaatttct cgaagctgcc ccatttcgcg tcgatggcgg cggccagttc
      361 gccggaaggt ttgccttgac ctttgggcgt gaaacccagc cagtagaagg tgtggttcca
      421 agtttgcgcc gcgttgttga acacgccgtc cgaagatttt ttcacaatct cttccaaagg
      481 caggttttca aattcggtgc ctttgatttg attgttcagg ttggtgatgt aggtttgatg
      541 gtgtttgccg tagtggaatt ccaaagtctc tttgctcaga tgcggggaca atgcgtccag
      601 ttcataaggc agttgcggca gcttatgttc cattttgtgc tcctaatatt gttttaaatg
      661 ttgtattttg gcagtgttgc tgcaaatagc tcagcagcgc acatatttta cctgttttga
      721 gggatggaaa ccaattaaac ctgctttacg ctataataga agattgcaat ttcggcacga
      781 cagataggat gtaccatgaa cgattacgca gctatgccgt ctgaagaccg tgaggtcggc
      841 gcattgtcgc tgcctccaca ttcgatggag gcggaacaat ccgttttggg cgggttgatg
      901 ctggaaaatc cggcctggga caggattgcc gatgtggttt cgggagagga tttctaccgc
      961 cacgaacacc gcctgatttt ccgatccatt gccaaattaa tcaatgagag ccgtcccgcc
     1021 gatgtgatta cggttcagga agatttgcag cggaacgaag aattggaagc ggcaggcgga
     1081 ttcgaatatc tgattacgct ggcgcaaaac accccgtctg ccgccaacat ccgccgctac
     1141 gccgaaatcg tgcgcgagcg ttccatcatg cgccaactcg ccgaagtggg gacggaaatc
     1201 gcccgcagcg catacaatcc gcaaggcagg gacgcggggc agcttttgga cgaggcggaa
     1261 aacaaagtat tccaaatcgc cgaaagcacc gccaaatcca agcagggctt tttggagatg
     1321 cccgatttgc tgaaagaagt cgtacagcgc atcgatatgc tctactcgcg cgacaatccc
     1381 gatgaagtta ccggcgtgcc gacagggttc atcgacctcg acaaaaaaac ctcgggtctg
     1441 caacccggcg acctgattat cgttgccggt cgtccgtcta tgggtaagac cgccttttcc
     1501 atcaatatcg ccgaacacgt tgccgtagaa ggcaagctgc cggttgccgt cttctcaatg
     1561 gagatgggcg gtgcgcaact ggtcatgcgt atgctcggct cggtcggacg cttggatcaa
     1621 agcgtcctga aaaccggcag gctcgaagac gaacactggg gtcgtctgaa cgaagcagtc
     1681 gtcaaactct ccgacgcgcc cgtgtacatc gacgagaccc cgggtttgac tgcgctcgaa
     1741 ctgcgcgccc gcgcccgccg tctcgcccgt caatttaaca ataagctggg attgatcgtc
     1801 atcgactacc tgcaactgat ggcgggatcc ggccgttccg acaaccgagc ttcagagctg
     1861 ggcgaaattt cacgttcgct caaagcgttg gcgaaagaat tgcaagtccc catcatcgcc
     1921 ctgtcgcaac tgagccgcac ggtcgaatcg cgtaccgaca aacgcccgat gatgtccgac
     1981 cttcgcgaat ccggtgctat cgagcaggat gccgacctga ttatgtttat gtaccgcgac
     2041 gaatactaca accaagactc gcccatgaaa ggccttgccg aatgtatcat cggcaaacac
     2101 cgcaacggtc ccgtcggtaa aatcttcctc acatggacgg ggcaattcac caaattcgac
     2161 aatgctgcct atattcccga ggaggcaaag atagaggatt aaatggctat ataaaaattt
     2221 attaggcgaa atcaggcaaa atcgtttaaa atcatgctta gagattgccc taaaaaataa
     2281 aacgcggtct tgaggcattt ttgcattcag cccgcatata attg
//
LOCUS       DAWFBO010000200         2317 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWFBO010000200 DAWFBO010000000
VERSION     DAWFBO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.200"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WGO87_001911"
     CDS             complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WGO87_001911"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0393222.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTINDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLQITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMVVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1627..2193
                     /locus_tag="WGO87_001912"
     CDS             1627..2193
                     /locus_tag="WGO87_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0393223.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          525 a          565 c          669 g          558 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc
       61 ggcgttcttt gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt
      121 tggcaacgaa gttgtccaag tcgggcagca gggaaacggc gcgaccgctg ttgatgatga
      181 cgaccaccac catcggtttg tcgcccagcc agtagcctgc caatgcgcgg acattgttga
      241 gtgtgcctgt tttcaggcgc agaagcccac cgctttgttt gaagcggttg cgtaaagttc
      301 cgtctgtgcc ggcgatgggc agcgtgtcga tgaaatcttg tgcaaacggg ctgaaataag
      361 ccgtttccaa catttgcgcc atcattctcg ccgttaccct ttcttttctg gacagacccg
      421 aaccgttttc caaaaccaaa tccgcaacat cgatgcccga cacggcaagt tcgcgccgga
      481 cggcagatgc cgcctgttcg gaaacggcgg gcagtttgcc gttgccgccg agtttgagga
      541 agacggaacg cgcaatcaga ttgtccgaac gcttgttcat gtccgtcaaa atttccttca
      601 tcggtttggc gtgtgcaacg gcaagcgtct gcgcgccttc cggcgtgtcg gctatgccga
      661 taccgtctga aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca
      721 gttcgtcaag cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat
      781 tgccgcgcaa tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgatcgaag
      841 ggcaggcagc ttgggaggcg gtaatttgca agttgttttg ggcgaaaata tgcggcaaag
      901 gcggatcggt gaggatgtcg gtactgtcgg cggcattgcg ttcggcgcgc accataacca
      961 taccggcaga cagcatagtt ggattggggg gcgtcataaa cggcgaaccg ctgtcggctt
     1021 cgaaatcgtc ggggctgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg
     1081 tgatattgcg tatgccttgt tcgcgcaact ggcgttggac ggcaagcagg ttttcctgat
     1141 tgaaaacggg gtcgccgctg cccgcccaat acaggtttcc gtcaagcgtg ccgtcgttta
     1201 tcgtaccgtt gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg
     1261 cggcaaacgc ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt
     1321 ggtcaatgat gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg
     1381 gaatgcggcc ggtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg
     1441 ccgctgtttt ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca
     1501 aagccggata agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat
     1561 atataataat cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga
     1621 atgattatgg ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg
     1681 cgcaaagtgg cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtc
     1741 gaaatcggac atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg
     1801 ccgctgcccg aaagctacac ggctttccgc gaaacgatta aggcttcgga cggcattttg
     1861 ttcgttacgt ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc
     1921 ggctcgaaac cgaatgccga cgtggcgtgg aaaaacaaac cggccggtat catcagccat
     1981 tccgtcggca agatgggcgg ttacagttcg caaaaaaacc tgcgccttgc gctgtcttat
     2041 ttcgatatgc ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacgctgttt
     2101 gatgaaaacg gcaaattgat cgactcggca agggattttg ttcagtcata tattaatcag
     2161 tttgttggtt tgattgaaag aaatgccaaa taaacaaacc aagtcgaaag gccgcaaaac
     2221 cggttcgggt tttgcggctt ttttatatgg gctgggcagc gtcaaggttt gccgtttgcc
     2281 ggataatgcg cggcaaatga ccggcgtgtt tatagcg
//
LOCUS       DAWFBO010000201         2297 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWFBO010000201 DAWFBO010000000
VERSION     DAWFBO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.201"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(36..992)
                     /locus_tag="WGO87_001913"
     CDS             complement(36..992)
                     /locus_tag="WGO87_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0393224.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            1228..2193
                     /locus_tag="WGO87_001914"
     CDS             1228..2193
                     /locus_tag="WGO87_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0393225.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT          486 a          617 c          734 g          460 t
ORIGIN      
        1 tgtttcaaat caaaatatct atgccgccgg ccccatcagc acttcttcac atccgaaggc
       61 aaaaatccgt aatgccgtct gaacgcttcg ttgaaccgtc ccgcgtggcg gtagccgcaa
      121 aagtgcatgg cggcttggac ggtgctgccg gattcgatga gggcgagcgc gtgttccagc
      181 cgcaggcggc gcaggcatcc ggcgacggtt tcgccggttt gcgctttgaa atagcgtttc
      241 aggtagcatt cgttcagtcc gacgcggcgg gcgatttcgg cgatggtcag cggacgggcg
      301 aattcgtgtt gcaggatgtc ggcggcttcg tctatgcgcc gacggcggta accgttgtcg
      361 tggcggcgga aggtgaagcg caataatcgg gcggagagtt ccagcgcggc ggcttcgtcg
      421 gcaagcaggc cgaagccgtc cgattcgaac gggcgttgca gcagtgggca ggccgccgcc
      481 gtcagtgccg ccgcgttttg cgccagccgt tgcagggcga atcggcctat cgtttgcggc
      541 gaaaacaggc gttcgtccag caagccttcg tcgtgccagc ggcgcagttt ttccagcgaa
      601 aaatccaaat gcagcgcgca catgccgctg ttgtcgggca gcagggtttc ggatacgtcc
      661 gccaactcgc cgcgtaccag ccagatttcg ccggcagacg ggcggtattc cctgccgccc
      721 atttgtaacc ggttctgccc cgacaccatg acgaacaagg cgcagttgtg gctgaaattg
      781 tggatttcgg tgggaaacgc gcccgttccg ccgccgcgca tccgcgacaa ggtgatgccc
      841 gagtcgaagc ggttgatgca catttccaga tgcaaaccgg gctgttttgc ctgcgcaatg
      901 agcgcgctgt cggaacagcc gtccaacgcc cagccggatt tatcggagcg gacataggtt
      961 tggtactggc ggtagatggc ggcggtgttc atgattggat aggaacgagt tgtctaacaa
     1021 atgaattaaa taggaattat taccaataat caagcgcagg gattggttga aacggaaaag
     1081 gtcgtctgaa agggtgtttc agacgacctt ttccgtatcg ggaatttgtt ttgccgtatc
     1141 gggaattttg cgttttgcgg cgtggtttct gcaggttgtt tgcttaataa taaacattct
     1201 tattcgtatg caaaggaacc gcacaccgtg aaaccgcgtt tttattgggc agcctgcgcc
     1261 gtcctgctga ccgcctgttc gcccgaacct gccgccgaaa aaactgtatc cgccgcatcc
     1321 gcatctgccg ccacactgac cgtgccgacc gcgcggggcg atgccgttgt gccgaagaat
     1381 cccgaacgcg tcgccgtgta cgactgggcg gcgttggata cgctgaccga attgggcgtg
     1441 aatgtgggcg caaccaccgc gccggtgcgc gtggattatt tgcagcctgc atttgacaag
     1501 gcggcaacgg tggggacgct gttcgagccc gattacgaag ccctgcaccg ctacaatcct
     1561 cagcttgtca ttaccggcgg gccgggcgcg gaagcgtatg aacagttggc gaaaaacgcg
     1621 accaccatag atctgacggt ggacaacggc aatatccgca ccagcggcga aaagcagatg
     1681 gagaccttgg cgcggatttt cggcaaggaa gcgcgcgcgg cggaattgaa ggcgcagatt
     1741 gacgcgctgt tcgcccaaac gcgcgaagcc gccaaaggca aaggacgcgg gctggtgctg
     1801 tcggttacgg gcaacaaggt gtccgccttc ggcacgcagt cgcggttggc aagttggata
     1861 cacggcgaca tcggcctacc gcctgtagac gaatctttac gcaacgaggg gcacgggcag
     1921 cctgtttcct tcgaatacat caaagagaaa aaccccgatt ggattttcat catcgaccgt
     1981 accgccgcca tcgggcagga agggccggcg gctgtcgaag tattggataa cgcgctggta
     2041 cgcggcacga acgcttggaa gcgcaagcaa atcatcgtca tgcctgccgc gaactacatt
     2101 gtcgcgggcg gcgcgcggca gttgattcag gcggcggagc agttgaaggc ggcgtttgaa
     2161 aaggcagaac ccgttgcggc ggggaaagag tagggagtcg tctgaaaacg gagcttccga
     2221 aggaagcggg gggtttctgc gaagctaaag tgcggtttca acgaattgaa aagcagcctg
     2281 tatgttgaaa ataccgc
//
LOCUS       DAWFBO010000202         2282 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWFBO010000202 DAWFBO010000000
VERSION     DAWFBO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.202"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            234..2012
                     /locus_tag="WGO87_001915"
     CDS             234..2012
                     /locus_tag="WGO87_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0393226.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVKTAVLATLLF
                     ATVQANATDEDEEEELESVQRSVVGSIQASMEGSGELETISLSMTNDSKEFVDPYIVV
                     TLKAGDNLKIKQNTNENTNASSFTYSLKKDLTGLINVETEKLSFGANGKKVNIISDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDMLLNTGATTNVTNDNVTDDEKKRAASVKDV
                     LNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGA
                     KTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSV
                     SLGAGADAPTLSVDDKGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
     gene            2145..>2282
                     /locus_tag="WGO87_001916"
     CDS             2145..>2282
                     /locus_tag="WGO87_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0393227.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YL"
BASE COUNT          698 a          566 c          562 g          456 t
ORIGIN      
        1 ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg gcacggaaac ttaccgaata
       61 aaacagtttc cttagattcc acgtcccaga ttcccgcctt cgcgggaatg acgagatttt
      121 aagttggggg aatttatcag aaaaccccca acccccaaaa accgggcgga tgccgcacca
      181 tccgccccca aaccccgatt taaccattca aacaaaccaa aagaaaaaac aaaatgaaca
      241 aaatataccg catcatttgg aatagtgccc tcaatgcctg ggtcgccgta tccgagctca
      301 cacgcaacca caccaaacgc gcctccgcaa ccgtgaagac cgccgtattg gcgacactgt
      361 tgtttgcaac ggttcaggcg aatgctaccg atgaagatga agaagaagag ttagaatccg
      421 tacaacgctc tgtcgtaggg agcattcaag ccagtatgga aggcagcggc gaattggaaa
      481 cgatatcatt atcaatgact aacgacagca aggaatttgt agacccatac atagtagtta
      541 ccctcaaagc cggcgacaac ctgaaaatca aacaaaacac caatgaaaac accaatgcca
      601 gtagcttcac ctactcgctg aaaaaagacc tcacaggcct gatcaatgtt gaaactgaaa
      661 aattatcgtt tggcgcaaac ggcaagaaag tcaacatcat aagcgacacc aaaggcttga
      721 atttcgcgaa agaaacggct gggacgaacg gcgacaccac ggttcatctg aacggtatcg
      781 gttcgacttt gaccgatatg ctgctgaata ccggagcgac cacaaacgta accaacgaca
      841 acgttaccga tgacgagaaa aaacgtgcgg caagcgttaa agacgtatta aacgcaggct
      901 ggaacattaa aggcgttaaa cccggtacaa cagcttccga taacgttgat ttcgtccgca
      961 cttacgacac agtcgagttc ttgagcgcag atacgaaaac aacgactgtt aatgtggaaa
     1021 gcaaagacaa cggcaagaaa accgaagtta aaatcggtgc gaagacttct gttattaaag
     1081 aaaaagacgg taagttggtt actggtaaag gcaaaggcga gaatggttct tctacagacg
     1141 aaggcgaagg cttagtgact gcaaaagaag tgattgatgc agtaaacaag gctggttgga
     1201 gaatgaaaac aacaaccgct aatggtcaaa caggtcaagc tgacaagttt gaaaccgtta
     1261 catcaggcac aaatgtaacc tttgctagtg gtaaaggtac aactgcgact gtaagtaaag
     1321 atgatcaagg caacatcact gttatgtatg atgtaaatgt cggcgatgcc ctaaacgtca
     1381 atcagctgca aaacagcggt tggaatttgg attccaaagc ggttgcaggt tcttcgggca
     1441 aagtcatcag cggcaatgtt tcgccgagca agggaaagat ggatgaaacc gtcaacatta
     1501 atgccggcaa caacatcgag attacccgca acggtaaaaa tatcgacatc gccacttcga
     1561 tgaccccgca gttttccagc gtttcgctcg gcgcgggggc ggatgcgccc actttgagcg
     1621 tggatgacaa gggcgcgttg aatgtcggca gcaaggatgc caacaaaccc gtccgcatta
     1681 ccaatgtcgc cccgggcgtt aaagaggggg atgttacaaa cgtcgcacaa cttaaaggcg
     1741 tggcgcaaaa cttgaacaac cgcatcgaca atgtggacgg caacgcgcgt gcgggcatcg
     1801 cccaagcgat tgcaaccgca ggtctggttc aggcgtatct gcccggcaag agtatgatgg
     1861 cgatcggcgg cggcacttat cgcggcgaag ccggttacgc catcggctac tccagtattt
     1921 ccgacggcgg aaattggatt atcaaaggca cggcttccgg caattcgcgc ggccatttcg
     1981 gtgcttccgc atctgtcggt tatcagtggt aagggctttt tcgcctgtct gctgttggga
     2041 caggcggaag gtttgaaggg aagggtggcg atttgccgcc tgagaccttt gcaaaattcc
     2101 ccaaaatccc ctaaattccc accaagacat ttaggggatg ttccatgagc accttctttc
     2161 agcaaaccgc acaagccatg attgccaaac acatcgaccg tttcccgcta ttgaagttgg
     2221 atcaagtgat tgattggcag ccgatcgagc agtacctgaa ccgtcaaaaa aaccgttacc
     2281 tt
//
LOCUS       DAWFBO010000203         2216 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWFBO010000203 DAWFBO010000000
VERSION     DAWFBO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.203"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="WGO87_001917"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="WGO87_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0393228.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          564 a          631 c          579 g          442 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgcagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc aacgacctcg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgacacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaaca
      481 aaatcgacaa gccgtccgct cgtccgagct gggtcatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttatgct tccggtctgt
      601 ccggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg ctgttcgata
      661 ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt atcttgaacg
      781 gacgaatcaa acccggccaa accgttgccg tcatgaacca cgatcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gtttggaacg cgtgccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggtatcggc gtaaccatca
      961 ccgacaaaga caatcccaaa ggcctaccga tgttgagcgt ggacgaaccg acgctgacga
     1021 tggactttat ggtcaacacc agcccgctgg cgggtacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 ataccgccga tgccgacgtg ttccgcgtat ccgggcgcgg cgagctgcac ctgaccattt
     1201 tgctggaaaa tatgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cgtatgaaaa cctgaccgtg gatgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggtttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccatgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggtcgta
     1621 tgttcgtatc acccaacgac aaaatctacg aaggtatgat tatcggcatc cacagccgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgcgccagcg
     1741 gcaccgacga agccgtgcgc ctgaccacgc cgatcaagct gacgctggaa ggtgcggttg
     1801 agtttatcga cgatgacgaa ctcgttgaaa tcacgccgca atccatccgt ctgcgcaagc
     1861 gttacttgag cgaattggaa cgccgccgcc actttaaaaa gctggattga tgtttactgg
     1921 attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat tttttattcg
     1981 gacgggcttt gcggcttctt gaagctgttt cagacggcgt ttttcctacc caatcaagaa
     2041 actgccgcca tttttccagc ggtatatcgc cccgtcgcgt ttcggtatcg ggttcggctt
     2101 cccggcagca tgtcgaacaa tgccgtttga aggaatacgc cttttgagtc cttacaagct
     2161 taggaagagg atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcaga
//
LOCUS       DAWFBO010000204         2163 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWFBO010000204 DAWFBO010000000
VERSION     DAWFBO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.204"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            373..885
                     /locus_tag="WGO87_001918"
     CDS             373..885
                     /locus_tag="WGO87_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0393229.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            965..2044
                     /gene="aroB"
                     /locus_tag="WGO87_001919"
     CDS             965..2044
                     /gene="aroB"
                     /locus_tag="WGO87_001919"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0393230.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          529 a          676 c          526 g          432 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gccaaggcga ggcaacgctg tactggtttt
       61 tgttaatcca ctatagtatt gataaacata ttatcttcaa tatattcaat tggataattg
      121 tttacctaag caaagataat tgccttttcc tgacaaataa gtgaaatcaa cggattgtca
      181 aaacacagcc tgaaataaaa aacctccctg atttctttta tttgtcctta aaatcagaaa
      241 ggttcgggat ggtcgggtta tttttccaaa cgtaccgccg ccctgccgat ttcgtataaa
      301 attccgccgt aacccgacaa gcccgaaccc tgtcgccccg aaaggcgggg tgtcaaacat
      361 taaggaattg tgatgaaaaa ctttaacggc aaactcatcc tcatcggact gatgggcgcg
      421 ggcaaaacca cgctgggccg gcaaatggcg cagcggctgg attaccgttt ttacgacagc
      481 gatcacgaaa tcgccgtagc cgccggcgtt cccatcccca ccatatttga aatggaaggc
      541 gaacagggat tccgttcgcg cgaaaccgcc atactcaaaa agctggttat cctgccccat
      601 atcgtcctgt ccaccggcgg cggcgcggtg ttaaaagaag aaaaccgcgc ccttatccgc
      661 aaaagcggca cggtcgtcta tctgcacgcc ccgcccgaaa ccctgctcga acgcacgcgc
      721 tgcgacaaca gccgtccttt gctgcaagtt gccgatcctt tggcgaaatt acgtgaactc
      781 tacgccgcac gcgaccccgt ttaccgccaa accgccgact ttaccgtaga atcggcaaac
      841 tgccgggaaa ccgtgcaaac cctgctcaaa cgcttatccc gataaaccgg catatgcgcc
      901 gcgcccagaa aaccaagccg cgccccgccc ggcgggccgg cggttcaaac tttaaggaac
      961 aacaatgaaa acactgaccg tacacacccc gtcccacagc taccccatct ttatcggcaa
     1021 cggactgctg ccgcaggcag gaagcctgct caaaccgcat ttgggcaaac gcgccgccat
     1081 catcgccaac gaaaccgtcg ccccgctcta cctcggcacg cttcagacgg cattggatgc
     1141 ggcaggcgta tcccatttca gcatcatcct gcccgacggc gaggcgcaca aaaactggca
     1201 gacgctcaac ctcatctttg acgggctgat gcaaaaccgc gccgaacgca aaaccacatt
     1261 aatcgcactg ggcggcggcg tgatcggcga catggtcggc tttgccgctg ccacctacca
     1321 gcgcggcgca ccgttcgttc aaataccgac cacactgttg agtcaagtcg actcatcggt
     1381 gggcggcaaa accgccatca accacccgct cggcaaaaat atgatcggcg cattctacca
     1441 gccgcaggcg gtgcttgccg acttggacac gctgcacacc ctgcccgtcc gcgaattgtc
     1501 cgccggtatg gcggaagtca tcaaatacgg cgcgctcggc gacatcggtt ttttcgaatg
     1561 gctcgaacaa aacatatccg gactgatgac gctcgatcgg gaaaaactcg cccaagccgt
     1621 gtaccgctgc tgccaaatga aggcagacat cgtcgcccaa gacgaaaccg aacagggcat
     1681 acgcgcatgg ctcaacctcg gacacacctt cggacacgcc attgaaaccg agatgggtta
     1741 cggcacttgg ctgcatggag aagccatcgc cgccggctgc gtgttggcgg cgcgtttgtc
     1801 cgaacaactg ggcaaaacct ccgccgcaga taccgcgcgg ctcgccgccc tgctcgaagc
     1861 cgctggactg ccgtccgcac cgcccgtgtt tgcctttgaa aaatggctcg aacatatgag
     1921 ccacgataaa aaagtcagcg gcggcatcat gcgctttatc ggtctgaacc ggctgggcga
     1981 agccaacatc accgaaatta ccgacacgga catcctccgc cgcaccctgc aaccgtatct
     2041 ctgatttcct ctgccgatgt gctgccgcgc gggtttgacg cacgatgatg attttccatc
     2101 atctttctcc gtaaaagcgg gaatccggtc cgttcggttt cggtcgtttc cgataaattc
     2161 ctg
//
LOCUS       DAWFBO010000205         2125 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWFBO010000205 DAWFBO010000000
VERSION     DAWFBO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.205"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(126..554)
                     /locus_tag="WGO87_001920"
     CDS             complement(126..554)
                     /locus_tag="WGO87_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0393231.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(535..969)
                     /locus_tag="WGO87_001921"
     CDS             complement(535..969)
                     /locus_tag="WGO87_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0393232.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLCRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKASGDDGVRKS"
     gene            complement(1142..1558)
                     /locus_tag="WGO87_001922"
     CDS             complement(1142..1558)
                     /locus_tag="WGO87_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393233.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
     gene            complement(1555..1746)
                     /locus_tag="WGO87_001923"
     CDS             complement(1555..1746)
                     /locus_tag="WGO87_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393234.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            complement(1790..2032)
                     /locus_tag="WGO87_001924"
     CDS             complement(1790..2032)
                     /locus_tag="WGO87_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393235.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
BASE COUNT          561 a          574 c          397 g          593 t
ORIGIN      
        1 acatttgaaa accccattaa accgtcttta acccatcgtc ccgaacggta gatgtttcat
       61 cccctgcgaa ccaaaaaaaa gaccgtctga atacagacgg ccaaagcctg gtcacacatc
      121 actgcctaaa ataaagctgc ctgctgtgcc gcaggtcggc tcatttcatt gataatcgta
      181 tatcccgttc gtgaagagat gccgtattta gggcatagct tcgtcatcgc cataagcccg
      241 ctctccttat caatatcgcg caatttgaca aactcctgat aaaacctatg gtttctcaac
      301 tgtattaacg ccttgccgca ccgtgggaca tacaattcct cgccaccata tacctgcaac
      361 agctcatgtg ttttcacttc gccgatggct tcgaccaaaa ttgccaaacg ctcggtgtcc
      421 accttgccct taccaaattt aaaccgcgcc ccgccaatcg ccttgaccag ctgttccgtc
      481 gctgccagtc cgatgacatc cacaatgtcc aacacggtat ccggcaataa atgttcaact
      541 ttttcgaacc ccatcatccc cactcgcttt ttccgttttc ctgttttccg caatctgcaa
      601 cgcagcaacc agtttatgta gctgcgtatc gtctaaatat tcgaccttat ccttaccaaa
      661 catccgccgc gccattgcgt gtgcatagtt ccaatgtttg ccgccgacgg tcagcagggc
      721 ttcgactttg tccaacattg ccgctgatga tgtccgacac agatgcggtt tgccgtgtgg
      781 gttacctttt gctttaggct taaatccgtg cgaccgcata tcagcgacaa cagactcaag
      841 ttcagaaaca tccatatccg cacacgaccg cttatccgtc acacgctcca acaccgcgcg
      901 ataggtaccg tcatccaagc ccagctcctt ttgagcaatc ttaattttcg caatcaacgc
      961 ccggcgcatt atctctccaa tacaacatat agtataaatt agcgcatttt atacaataaa
     1021 atacaatata tagtattaag ccgatgtttt tttgcgaaac ggacagacat aaaaaatgcc
     1081 gtctgaaaac gagctttgcg agtttcgcca aaacgtttca gacgaccttt tttactacaa
     1141 tctaatcact gtctttttcg cgccgttgct tttccaaggt ttgcgcttta cctctgttga
     1201 ttcctgcaaa atatcccaaa gctaccgccc cgccgatttt gacaatttca atagcgacgc
     1261 tgtccttatc gcgccataac gcagtaatca gcacggcggc aatcagaaaa gaaatagcaa
     1321 tcacggcatt cagacggcgt ttgtgtacct ctttgaaaat ctcaccatgt tgctgttcac
     1381 cctgtttttg cgcttcgatg gtagccagtg caatgcgttc gtgagattgg atttcgtcct
     1441 tacggctttc caactccttc tgtttgactg caatatcctg tttttgaagt tctaaaaact
     1501 cctgaagcat tttctgctgc tcatcggccg gcagattctc ggatttttcc ccgctcataa
     1561 atcaaccgcc actaaggttt tgtaacggac tttgaatttg ccgtaacccc gagcccctat
     1621 tttcggcggc acaaattcca cggacgcaat attgcccgtc ttacggtctt tacggacttc
     1681 ttccaaataa tcggcagcat tgagcagccg ttcgcggact gccgtatatt ctttgacggt
     1741 attcattttt caacctcgcc caaatagtgc aaatattagc atatgggatt tatttttcaa
     1801 gaaaatcaaa tccatataaa acaaccgtat aatattttaa tactatatat ggtaaatttc
     1861 ttctaaaaat acacactaaa ataccatatc ttgataaccg caacgccgca caaatggcag
     1921 ggcattgatg cccgacaaca aaaaatgccg tctgaaacag cattcgagct gttttcagac
     1981 ggcatttttc tttgattttg ttggggaaag ggttgacttt taacgtgtcc acgtttataa
     2041 ttcaattcat cggcgtagtg agggccgata cgggaaaagc cccaccgaag cggggctcaa
     2101 ctgggagaaa gaagatgaat aaacg
//
LOCUS       DAWFBO010000206         2122 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWFBO010000206 DAWFBO010000000
VERSION     DAWFBO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.206"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            123..1508
                     /locus_tag="WGO87_001925"
     CDS             123..1508
                     /locus_tag="WGO87_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0393236.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     DFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1528..2046
                     /locus_tag="WGO87_001926"
     CDS             1528..2046
                     /locus_tag="WGO87_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0393237.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          448 a          646 c          570 g          458 t
ORIGIN      
        1 ttcaaataaa caggaatata acttcagaca aacaacttac cgcccgattt gtgctatcgt
       61 tttcgcacaa cttaaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acgtactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttctg gtcggcctgg
      361 gcgcgatgct cggacgtttg gtcgaaacat ccggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga acctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcgacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgccgccgc gctgatggct cctgccgttg ccgccgccga ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgc ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttcgcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccctat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgaaacag
     1741 gcgcaaaacg gtgcgaacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gccttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggagtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggattaca tt
//
LOCUS       DAWFBO010000207         1999 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWFBO010000207 DAWFBO010000000
VERSION     DAWFBO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.207"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            299..1756
                     /locus_tag="WGO87_001927"
     CDS             299..1756
                     /locus_tag="WGO87_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0393238.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          453 a          594 c          457 g          495 t
ORIGIN      
        1 tgcgcccttc gacgtggcgg atatataagg aagtgatttt ccatctaagt aaaaaccgcc
       61 ctatcggata agcccttaac agaaaaggct ttacccgcgc cgtatcggaa cacatcctct
      121 aaaatacaat ccgttgaatt gaaaaaaata taaaaacatc cgcccgcgaa aaacggcagc
      181 gcgtcgtttg acaaagattg aaaatatcgg ttaaaaaccg attttcatac aaaaaacacc
      241 gctgccgtcc gcatccgttt cagacggtat tgagagaaaa tcttttagga gaacctttat
      301 gtcccggcat cccgccccca ccggagaaaa aacattcttc ggccacccct tccagctttc
      361 caccctcttc catatcgaat tgtgggaacg tttttcattt tacggaatgc agggcatcct
      421 gctgatttac ctctactaca ccgccgacaa aggcggcttg ggcatagaca aaaccctcgc
      481 cggcggcatt gtcggcgcat acagcggcag cgtgtacctg tccaccattt tgggggcgtg
      541 gtttgccgac cgagtatggg gtgcggaaaa aaccctcttc ctctcgggca tcgtcgtgat
      601 gctcggacac atcgtccttg ccgccgcccc gggcctgtac ggccttttaa tcgggctgat
      661 attcatcgca ttgggcagcg gcggcgtgaa atctacggcc agttctatgg tgggcgcatt
      721 atacgaacaa gacgaaatgc gcccgctgcg cgatgcggga ttttccattt tctacatcgc
      781 catcaatatc ggcggcttcc taggcccgct gctgaccggc ctgcttcaag aaaacatcgg
      841 tttccattat ggtttcggcg cggcagcggt cggtatggca ttcggcttgt ggcgttattc
      901 cttgggacgt aaaaacctgc cccaccccac cgtcccccat ccgctttcaa aaggacaggg
      961 caaaactgcg gccgccgtcg gcattgctct catcgccgca cttgcaaccg ccatcaaaac
     1021 ggggcttgtc aacctcgaca atttctccgg catcctctta tctaccgtca tccttgccgt
     1081 cattgcctat ttcgcccgcc tgctgaccaa cccccgcgtc agttccgaca acaagcggca
     1141 catcatcgcc tacatcccgc ttttcctgac catctgtatg ttttgggccg tctggtttca
     1201 gatttacacc gtagcaaccg tctatttcga cgaaaccgtc aaccgcacca tcggttcgtt
     1261 taccgtgccc gtcgcctgga aagattccat gcaaagcctg tgggtcatcc tgttttccgg
     1321 actgatggcg gcaatgtgga caaaaatggg gcgcaaacag cccaaaaccc cgctgaaatt
     1381 cgctatggcg gtatttgtta ccggcgcgtc gtttttggga ttcgtcccct ttatttccgc
     1441 cggcacgccg atgcctattg cggttttcgc actgattgtc cttgccatca cgataggcga
     1501 actgatgatt tccccgattg cgctgtccat ctccaccaaa atcgcaccgc ctttattcaa
     1561 aacccaaatg gtcgccctta atttccttgc cttttcatta ggcttcactt tgggcggcgt
     1621 attgtttgaa aaaggctatc aggcgggcga cgaaatcggc ttctatcggc tgctgttcta
     1681 catcggcgca gccacaggct tcctgctgct cctgctcgtc cccaaattga acaaaatgct
     1741 cgaaggcaca gactaagtcc cgccccgatg ccgtctgaac ccttcagacg gcatttttcc
     1801 gcataatgaa accaaaccgt ttccacccga caggacaggc tccccccaac cggaaggcag
     1861 cctgccgatt gtcatttaaa tatctgactg attccgtctg tttgcaaggg aaatagccgt
     1921 ttatttccgc ttgggcggaa accggttcta ttgagtaaaa ggcattttat ccgactaaat
     1981 tagtgctgca tcaacggaa
//
LOCUS       DAWFBO010000208         1940 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWFBO010000208 DAWFBO010000000
VERSION     DAWFBO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.208"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(6..1262)
                     /locus_tag="WGO87_001928"
     CDS             complement(6..1262)
                     /locus_tag="WGO87_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0393239.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLQNLKIDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1610..>1940)
                     /locus_tag="WGO87_001929"
     CDS             complement(1610..>1940)
                     /locus_tag="WGO87_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393240.1"
                     /translation="VKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAYCLSM
                     LPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFTLAKE
                     IGKQKNI"
BASE COUNT          456 a          417 c          478 g          589 t
ORIGIN      
        1 ccggtttatt tgcgttgcaa tacggcggcg aagaagccgt cggtttggtg ctttgccgaa
       61 tcgaggcgca ggtattttcc ggtatccaaa tcaatcttca ggttttgcag cagttcggcg
      121 cagttgacgg gttcaaattc gggatgttcg gacaggaaac gttcgacttg cagctcgttt
      181 tcttcgggca ggatgctgca agtggcgtaa accaagcggc cttgcggttt taccagtttg
      241 gaggcagcat cgaggatgct gtgttgctgt tccaaaaggt tggccacggt ctcggcagat
      301 tggcggtatt tgaggtcggg attgcggcgt aaagtaccca aaccggagca gggcgcgtcc
      361 accaacacac ggtcggcttt gcctgccagt cgggcgatac ggctgtcgtg ttcgctgctg
      421 atgcgttcgg ggtggatatt ggtcagtccg gcgcgggtca tacgcggttt gaggttggca
      481 aggcgttttt cggcgatgtc gaaggcgtag attctgcctt tattggccat ttgcgcaccg
      541 acagccaagg ttttgccgcc ggcaccggca cagaaatcga caatgatttc gccgcgtttt
      601 gcgcccacca ataaagcaag cagctggctg ccttcgtctt gaacttccag cgtaccgtcc
      661 aaaaacagct cgtgtttgtt aagcgcgatt ttgtttttca ggcggatgcc ccaaggcgaa
      721 taaggcgttg cctctgcatc ggcactttcg gcttgcaaca atggcagcac tttatcgcgt
      781 ttgcctttca aggtattgac gcgtatatcg agcggcgcag cttggttgat gctgcggccg
      841 aaggcgagga tttcttcttc gcgccaatgc tgctgcagct gttctaccag ccattgcggc
      901 aattctgcgg cggtattcag gccgtctgaa aactcggttt tacgggcttt caaattgccg
      961 agaaactcta tttcttcttc atcaagcagg tctttgattt ggctgatgtt ggtgcttctg
     1021 ccgagaacca gtgcggcgag tgcggctttg cgcggctgcg cgtgcggacg gcgcagggcg
     1081 gtactgattt tttgatagtg gcgcagcgcg gcaaaggcgg tttcggcgat ttcgtggcgg
     1141 tcttggcggc cgagcttttt gtgttcgcgg aaataggcgg agaggacggc atcggaaggc
     1201 tgtttgaaag tcagcatttc agccaaaact ttggcggtat ggtcgagttg tgcggcgttc
     1261 ataatgtaat gggttctctt actgtgggtt attcataatc aatctaacgt ccttaaaata
     1321 acaatagcat ctttatcatt acttagtatt ctatccgcaa taagaaagat gtgttaaaag
     1381 tgcgcaatcg ggatttgtct aaatttctcc gttaactaca aaggcagcct gaaaaaagtt
     1441 ctattttcag gtcgcttgaa cagcatccga tataatcaca tcagcgattt cattttgtaa
     1501 tcactcccta aaacatgagg aaaaaaacaa aatttatttt cttcgttcag tatatctatc
     1561 ctaaatatgc catcactcca ttgagttttt aatttgtatt tcgctcttgt taaatgtttt
     1621 tctgtttgcc gatttctttt gctaatgtaa aaaatactct accactctca ttggttaaag
     1681 ttttcaagta ttcatcaaca tcagtaacat taataacaac tgtttcattt gtatgtttat
     1741 taaattctaa atcaataata atattcccat taaccaatat ttcatatata tcttcttctt
     1801 cagggagcat agacaaacaa taagcacttg aagctgaaaa ctcttttttc agatactgaa
     1861 gaagaaaagt atttttaatt aaaatattat taaatttatc taatgattgc ttgacttctt
     1921 tagttgtcca atttttaaca
//
LOCUS       DAWFBO010000209         1935 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWFBO010000209 DAWFBO010000000
VERSION     DAWFBO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.209"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            238..1778
                     /locus_tag="WGO87_001930"
     rRNA            238..1778
                     /locus_tag="WGO87_001930"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          518 a          415 c          589 g          413 t
ORIGIN      
        1 tgctgcagaa gcggcggacg aactgaaaag tatagcacag aatgttgggg atatcgagag
       61 atatcttgac aggcggaaga gatactttat aattcgcaac gctctttaac aaaacagatt
      121 accgataagt gtgagtgcct tgagcctcac actgtttgaa agacagacaa gataatgttt
      181 tgaacattgt cctgttggtt tctttgaagc agaccagaag ttaaaaagtt agagattgaa
      241 cataagagtt tgatcctggc tcagattgaa cgctggcggc atgctttaca catgcaagtc
      301 ggacggcagc acagagaagc ttgcttcttg ggtggcgagt ggcgaacggg tgagtaacat
      361 atcggaacgt accgagtagt gggggataac tgatcgaaag atcagctaat accgcatacg
      421 tcttgagaga gaaagcaggg gacctttggg ccttgcgcta ttcgagcggc cgatatctga
      481 ttagctggtt ggtggggtaa aggcctacca aggcgacgat cagtagcggg tctgagagga
      541 tgatccgcca cactgggact gagacacggc ccagactcct acgggaggca gcagtgggga
      601 attttggaca atgggcgcaa gcctgatcca gccatgccgc gtgtctgaag aaggccttcg
      661 ggttgtaaag gacttttgtc agggaagaaa aggctgttgc taataccagc ggccgatgac
      721 ggtacctgaa gaataagcac cggctaacta cgtgccagca gccgcggtaa tacgtagggt
      781 gcgagcgtta atcggaatta ctgggcgtaa agcgggcgca gacggttact taagcaagat
      841 gtgaaatccc cgggctcaac ccgggaactg cgttctgaac tgggtgacta gagtgtgtca
      901 gagggaggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag gaataccgat
      961 ggcgaaggca gcctcctggg acaacactga cgttcatgcc cgaaagcgtg ggtagcaaac
     1021 aggattagat accctggtag tccacgccct aaacgatgtc aattagctgt tgggcaactt
     1081 gattgcttag tagcgtagct aacgcgtgaa attgaccgcc tggggagtac ggtcgcaaga
     1141 ttaaaactca aaggaattga cggggacccg cacaagcggt ggatgatgtg gattaattcg
     1201 atgcaacgcg aagaacctta cctggtcttg acatgtacgg aatcctccag agacggagga
     1261 gtgccttcgg gagccgtaac acaggtgctg catggctgtc gtcagctcgt gtcgtgagat
     1321 gttgggttaa gtcccgcaac gagcgcaacc cttgtcatta gttgccatca ttcagttggg
     1381 cactctaatg agactgccgg tgacaagccg gaggaaggtg gggatgacgt caagtcctca
     1441 tggcccttat gaccagggct tcacacgtca tacaatggtc ggtacagagg gtagccaagc
     1501 cgcgaggcgg agccaatctc acaaaaccga tcgtagtccg gattgcactc tgcaactcga
     1561 gtgcatgaag tcggaatcgc tagtaatcgc aggtcagcat actgcggtga atacgttccc
     1621 gggtcttgta cacaccgccc gtcacaccat gggagtgggg gataccagaa gtaggtaggg
     1681 taaccgcaag gagtccgctt accacggtat gcttcatgac tggggtgaag tcgtaacaag
     1741 gtagccgtag gggaacctgc ggctggatca cctcctttct agagaaagaa gaggctttag
     1801 gcattcacac ttatcggtaa actgaaaaga tgcggaagaa gcttgagtga aggcaagatt
     1861 cgcttaagaa gagaatccgg gtttgtagct cagctggtta gagcacacgc ttgataagcg
     1921 tggggtcgga ggttc
//
LOCUS       DAWFBO010000210         1892 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWFBO010000210 DAWFBO010000000
VERSION     DAWFBO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.210"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            237..1196
                     /locus_tag="WGO87_001931"
     CDS             237..1196
                     /locus_tag="WGO87_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393241.1"
                     /translation="MYQNYDIVILSLGNGLLTRFFRNSSKLNLTLPIVITLFPGVIFG
                     DSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPILRKKHHCSDGNKV
                     YFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDITVHEDKYSYAELA
                     KNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGVISDLGVDKKYAN
                     THFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSSKEFNQLLEVTSTQPPSFDN
                     YFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1244..>1892
                     /locus_tag="WGO87_001932"
     CDS             1244..>1892
                     /locus_tag="WGO87_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEZ0393242.1"
                     /translation="MQKIRKAVFPVAGMGTRFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDL"
BASE COUNT          636 a          310 c          334 g          612 t
ORIGIN      
        1 acttaatatc taatatttaa aatacaatga ataaaaaacg agtcctatta attgctagtt
       61 atgattcttt tttgaatact ggatatgctg ttgcaaaaga aattaactat tcaacaatcg
      121 atatttatat ccaccaaaca aaaaaaaata ttctttcgga atcacaatta agtttaaatg
      181 ggctttcttt aaaagaaatc aatttttttc atattgatga ttatttcgac aaattaatgt
      241 accagaatta tgatattgtt attctatcat taggtaatgg tttactgacg cgtttttttc
      301 ggaatagtag caaacttaat ttaacattac ctatagtaat tactctattt ccaggggtaa
      361 tttttggtga ttcagaaagt attataacta ggttaggtgc taatatttta ttgttcaata
      421 attattatga ttaccaaata ggcaataagt tcaaagaacg ttacaactta tcgtgtacaa
      481 atattttata tggttatcct atcctacgaa aaaaacacca ttgctctgat ggcaataaag
      541 tttatttcat tgatcaagta aaaattccag atggaaaaaa agaaagatta ttaattctaa
      601 aaaaattaat agcactggct aatcgctatc cacataaaga tttagtacta ttattacgtg
      661 tccatcaaaa tgatatcaca gtacatgaag ataaatactc ttatgctgaa ttagcaaaaa
      721 atatgaaatt gcctgataat tttaaaatag agagaaaacg gacacaagat gccttacaag
      781 aaatgggata ttgtttatca ttctcttcaa caatgttatt tgaagctgaa agctgcggaa
      841 ttcctattgg tgtcattagt gatttagggg ttgacaagaa atatgccaat acacatttca
      901 aagatagtgg tgttttggtt aatttagata cggtagactt ggactctcca aataccatta
      961 atccaacttg ggctgaacta tatactaatc cagatagcct ctcttcaaaa gagtttaacc
     1021 aattattaga ggttacttct actcaaccac ctagttttga taattatttt attgcagtta
     1081 atgacgttca gcctgtatct atggttctgt tacgtaaatt taaaaagctt atatatcatc
     1141 ctaagcaatt ctttgttgat tcaaaactat ataaattaat taaatttact aaataatttt
     1201 tactatgggt tgccttattt atttcaaagg agtgacataa aaaatgcaaa aaattcgtaa
     1261 agctgtattc cctgtagctg gcatgggaac ccgattctta cctgccacga aagccagcgc
     1321 taaagaaatg ctgccaatcg tggacaaacc gttaattcaa tatgctgcgg aagaagccgt
     1381 tgccgcgggt tgtactgaat tgatttttat caccggccgt aataagcgta atattgaaga
     1441 ccacttcgat aaagcctatg agttggaaac tgaactggaa atgcgtgaaa agcataaatt
     1501 gcttggctat gtgcaaaaaa ttctgcctga tcatgtcagc tgtattttca ttcgtcaggc
     1561 agaagctttg ggcttgggtc atgcggtatt atgcgcccgt cctgctgtcg gtaatgagcc
     1621 ttttgccgtg attttggccg atgacttgat tgatgcacca caaggcgcac tcaagcagat
     1681 ggtagatatt tacaatcaaa gcggcaacag cattttaggg gtagaaacag tcgatccttc
     1741 gcaaaccggt tcatacggca ttgtcgaagt ggaacagctg aaaagctatc agcgtgtaac
     1801 caatattgtg gaaaaaccaa agcctgaaga tgcgccgtca aatttagcgg tagtcggccg
     1861 ttatattttg acaccgcgta tttttgattt gc
//
LOCUS       DAWFBO010000211         1835 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWFBO010000211 DAWFBO010000000
VERSION     DAWFBO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.211"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            31..243
                     /locus_tag="WGO87_001933"
     CDS             31..243
                     /locus_tag="WGO87_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HEZ0393243.1"
                     /translation="MELETVVPPEEGEGEREGADGEMPEYLELHGGREDDISAPSQPE
                     PPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            278..1789
                     /gene="ubiB"
                     /locus_tag="WGO87_001934"
     CDS             278..1789
                     /gene="ubiB"
                     /locus_tag="WGO87_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0393244.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT          403 a          499 c          494 g          439 t
ORIGIN      
        1 cttgtcctga tttttgttaa tccactacat atggagttag aaacggtcgt gccgccggag
       61 gaaggtgagg gcgaaagaga gggcgcggat ggggaaatgc ccgaatatct tgaacttcat
      121 ggcgggcggg aagatgatat ttccgcacct tcgcaacccg aaccgccgac ccgcaatatc
      181 aaaccgctgc ttttcgggct gctgattgcc tttttgggct atctgctcgg taagattttt
      241 tgattgtccg ataaatgctg tattcgggat tttatatatg aaatggttga aacgcctgac
      301 ggtcattgtc gggacttttt accgctatcg gctggcaggt ctgtgtgctt cgctgatggg
      361 tagcggttgg atatgcgctc tgctgaaaat gatgccgcag tcgtccaaat tgaaaaacga
      421 accgcctgcc gtccgtctgc gccttgcctt ggaaagcctg gggccgattt tcatcaagtt
      481 cgggcaggtt ttgtccacac gccccgattt gattccgcac gattacgccg tcgaactggc
      541 aaagctgcaa gacaaagtgc cgccttttga cgcgcggctt tcgcgtgaac aaatcgaaaa
      601 atcgttgggt cagtccatcg aaaagctgta tgcggaattt gaaaccgagc ccatcgccag
      661 cgcgtccatc gcccaagtac acaaagcccg cctgcattcg ggcgaacgag tggcggttaa
      721 agttttgcgc cccaaccttt tgccccttat cgaacaggat ttgtcgctga tgcgtttcgg
      781 cgcggcctgg gtcgaacgct tgttttcaga cggcaggcgt ctgaagccgc tcgaagtggt
      841 ggcggagttc gacaaatacc tgcacgacga gttggatttg atgcgcgaag ccgccaatgc
      901 cggacagctc gggcgcaatt tccaaaacag caatatgctg attgtgccca aggtgtttta
      961 cgactactgc accagcgacg tgctgacaat cgaatggatg gacggcacgc cggttgccga
     1021 catcgccaaa ctcaaagcag acggcatcga tttgcacaaa ctcgccgatt acggcgtgga
     1081 aatctttttc acgcaggtct tccgcgacgg ctttttccac gcggatatgc accccggcaa
     1141 tattttggtt gccgccgaca accgctacat cgccctcgat ttcggcatcg tcggcacgct
     1201 gaccgattac gacaaacgtt atctcgccat caatttcctc gcctttttca accgcgatta
     1261 ccggcgcgtc gccaccgccc acatcgaatc gggctgggtg cctgccgaca cgcgtgcgga
     1321 agaattggaa gccgccgtcc gcgccgtgtg cgaaccggtg ttcaacaaac cgatttcgca
     1381 gatttccttc ggcttggtgc tgatgcgcct gtttgaagtc agccgccgct tcaatgtcga
     1441 aatccaaccg cagcttgtgc tgctgcaaaa aacgctgctc aacatcgaag gcttgggccg
     1501 ccagctcgat cccgatttgg acttgtggaa aaccgccaaa ccgtttttgg tgaaatggat
     1561 gaacgggcag gtcggcccta aagccctttg gcgcaacctc aaaaacgaag cccccgactg
     1621 ggcgcaaatc atcccttcat tgccgcgcaa aatcagtgcg ttgattgatg aaaaccgcca
     1681 gcaggaaatg cgtgatgcct atatccattt ggtcaaagtg cagcagcggc aaagcctgtg
     1741 gctggctgtg attgcggttg ttttgctgct gattttgctt ttgaaatagg ctttgtccga
     1801 atcatcgccc gactccgccc gtttataagg aaatc
//
LOCUS       DAWFBO010000212         1825 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWFBO010000212 DAWFBO010000000
VERSION     DAWFBO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.212"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            324..1769
                     /gene="zwf"
                     /locus_tag="WGO87_001935"
     CDS             324..1769
                     /gene="zwf"
                     /locus_tag="WGO87_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0393245.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT          499 a          422 c          483 g          421 t
ORIGIN      
        1 aaggtatacc gtctgaaagt gggaagcggg tgttattata gtacaaaatt tgtaattttg
       61 ttacaaaaaa atcagacatc gaaaagaaat ttgatttaaa tcgcagaatc tttcgattgt
      121 tgtaacggaa taggggaaac ctgcccgatt tttggactag gtcaaataaa ttgtaaaaat
      181 tgggaataga caaaaagtgt agcggtgtgt agtattacta ctcattatgc cgcctgcctt
      241 tgtttccgat attgcgatgc agcgtgatgg gggaaacagg tttcagacgg catcgaaaaa
      301 ttctttcctg acgagataat acgatgagta cacaaacaaa ttttgatttg gtgttgttcg
      361 gtgcgaccgg cgatttggcg atgcgcaagc tgttgccctg tctgtaccag gcgcatgttg
      421 ccggtctgct tcatcccgaa gggcgtattt tgggggtcag ccgcagcgag ttggataccg
      481 aaggttttct ggcgaaggta gaaaccagtt ccaaaatcca tgtgaaagaa aatttttccg
      541 atgaggcttg ggcatcgttt gtcgaacgtt tcgcatatct caaagtcgat gttacgcagc
      601 cggacgattt tgccgctttg ggcgatttgg tcaaagcgcg caaagaaacc gacaatgtcg
      661 tcatctatct ttccaccgcg ccgaaattct tcgcgcaaac atgtgaaaac cttgccaaaa
      721 tcggtttgaa cgccgacaac gtgcgtgtgg ttttggaaaa accgttgggt acggatttgg
      781 cttcttccca gcaaatcaat accgatgttg cgcgttattt caaagaaggg caaatttacc
      841 gcatcgacca ctaccttggc aaagaaagcc tgcaaaacct gcttgcgctg cgttttgcca
      901 atgtaatgtt cgagccgctg tggaacaata aatacatcga aagcgtgcag ttgaccatcg
      961 ccgaacagct cggtgtggaa gagcgcggcg agttttacga cattactggc gccttgcgcg
     1021 atatggtgca aaaccacttg atgcaaatgc tgtgcatgac tgcgatggaa gcccccgcca
     1081 gcttggatgc cgacgcggtg cgcgatgaaa aagtcaaagt catcaagtca ttgaagccgc
     1141 tgaccgtcga atctgtcaat gaaaatgtcg tgcgcggaca atataccgcc gccaaaggca
     1201 tgaacggcta tcttgaagaa atcaacgttc cgcaagacag ctttaccgaa acctacgtcg
     1261 ccattaaagc cgaaatcgaa aacgaacgct ggaagggcgt tcccttctac ctgcgtaccg
     1321 gcaagcgcat ggcgggcaaa gtggcggaaa tcgttttgaa cttcaaagat ttgaacagcc
     1381 atatttttga aggcagccgc accgcgccca accggctcgt tatcgagttg caaccatatg
     1441 aatccgtgcg cctctatacg cagatgaaaa ccccgggggc aggaaataag gtcgaaaccg
     1501 tgccgctggc aaccgatttg ggcaaagcat tggaaagccg ccgcgcggaa gcttacgagc
     1561 gcctgctgct ggatgtgatt aacggcaaac tcgctttgtt caaccgccgc gacgaacttg
     1621 aagccgcgtg ggaatatgtg atgccgattt tggaaaactg gacaaataac accacgccgc
     1681 cgcacggcta cggcgcacac tcgtgggggc ctgaagccgc gcgcgaacta ttggcgcgcg
     1741 acggacacaa gtggcacgaa gagcagtaat acaataatgc gttcagacgg catggggttt
     1801 gaaatgccgt ctgaacgcaa gtaaa
//
LOCUS       DAWFBO010000213         1783 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWFBO010000213 DAWFBO010000000
VERSION     DAWFBO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.213"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            216..1688
                     /gene="pyk"
                     /locus_tag="WGO87_001936"
     CDS             216..1688
                     /gene="pyk"
                     /locus_tag="WGO87_001936"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0393246.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          459 a          518 c          483 g          323 t
ORIGIN      
        1 agctccaaaa ataaatttag cctaagtcat aaaaccgtgt aattttgcac caaaaaagac
       61 ggtttgcagg taaaatatca aactattaat ttaaatcata tttttacaga atattccgcc
      121 gcattcatca aatggacatc aaaccggttc caaatttctg taattttgca acaaaatacc
      181 gcaaaacacc cgattgagac cgaaaggact ttcatatgaa ccagacaagc cgcgatctga
      241 cccgcatcag ccacaacacc aaaatcgtcg ccaccttggg gccgggcagc aacaacgtcg
      301 aactgttgga agacatgatc cgcgtcggcg gtctgaacgt cgtccgcttc aacttcagcc
      361 acggcacgcc cgaattccat caggaaaacg cccgcattgt gcgcgaggcg gcaaaacgcg
      421 ccggacagga aatcgccatc attgccgacc tgcaagggcc gaaaatccgc gtgggcaaaa
      481 tcgccggcgg cggcatcgaa ttgaacaaag gcgaaacgct ggtactggat gccgcgctcg
      541 aaggcgaagg cacgcgcgag gcggtcggtt tggactaccg cgacctgccc gacgacgtta
      601 ccgcaggcga cgtattgtgg ctggacgacg gcctgctgac cctgaccgtg gaatccgtcg
      661 aaggcagcag gattatcaca agggtggaaa acagccacgt cctgaaaagc aacaagggca
      721 tcaacaaacg cggcggcggt ctatctgcag gcgcgttgac cgaaaaagac ttccgcgacc
      781 tgaaaaccgc gattgccatc ggttgcgact acctcgccat cagctttgtg aaatccgccg
      841 aagatttgca tatcgcgcgc gccaaagtcg aagaagaaat gaagggcagc actgccgtgc
      901 gccccggttt ggtttccaaa atcgaacgcg tagaagcgat tgaaaacctt gatgaaatca
      961 tcctcgccgg cgacggcatt atggttgcgc gcggcgactt ggcggtcgaa gtcggacacg
     1021 ccgccgtccc cgccctgcaa aaacggatga tccgccgcgc ccgcgagttg cgccgcttca
     1081 gcattacggc gacgcaaatg atggaatcga tgattaccaa ccccgtgccg acccgcgcgg
     1141 aagtcagcga tgtggcaaac gcggtattgg acggcaccga tgcggtgatg tgttccgccg
     1201 aaaccgccgt cggcgcgtat ccgtttgaaa ccgtcagcca aatggcgatt atctgcgcgg
     1261 ctgcggaaaa agagcaggat tcgctcaacg gcgttgccga acaggtcgag tatcccgaag
     1321 cggtcagcac caacctggcg gttgccggcg gtgcggtcag cgtggcgcgc gcggttcacg
     1381 ccaaagccat cgtcgccctg accgaaagcg gttcgaccgc ctttgaaatc agccgccaca
     1441 acatcaccct gccgattttc gcgctgaccc cgagcgtttc cgcccaacgc cgtatggcga
     1501 tgtaccgggg cgtgcgcccg ctgattttgg caaccagcac cgaccacgac acggcgttaa
     1561 acgaagtcga aaccatgctg gtggaacaca acatcctgca ttccggcgac caatacatca
     1621 ttaccagcgg ctcgcaaatg cgcgaatccg gttcgaccaa tacgctggaa gtgctgcgcg
     1681 tcaaataatc cgccctgagt ggaaaatgcc gtctgaagtc gatgcccgag gcttcagacg
     1741 gcattgtttt aggcgcgatt tatagtggat taacaaaaat cag
//
LOCUS       DAWFBO010000214         1752 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWFBO010000214 DAWFBO010000000
VERSION     DAWFBO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.214"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(110..766)
                     /locus_tag="WGO87_001937"
     CDS             complement(110..766)
                     /locus_tag="WGO87_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0393247.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALVAAAILLLS
                     "
     gene            831..1721
                     /locus_tag="WGO87_001938"
     CDS             831..1721
                     /locus_tag="WGO87_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0393248.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQETLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWMAGCGLGTDKAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          396 a          419 c          579 g          358 t
ORIGIN      
        1 tgggacggaa aaacggcctg aagccgtttc gggcattcag accgtttgcg cggagagggg
       61 atgccgtccg aagggcgaaa agggcttcag acggcattga tgtcgggttt caggacagga
      121 gcaggatggc ggctgcgaca agcgcggcaa ccgataatgc ggcggcaagc gcggttttgc
      181 cggcaaagcg gattgaggtt ttgccttcga tgtatttgaa gccggttatc atcgggagga
      241 cgaggttttt ctttttgaac acgcggtatg cggcgacggc ggcgatatga atggcggaaa
      301 aaacggcgag cagcttgaaa aagttgaggt ggattttccg cataaggctg cccgtatgct
      361 cggaaaccaa atggttgagg tagccgttgg tgctgaaggt gttttcatcg gcggcaaaaa
      421 gccctgtgcc gacttgaaat gagacggcgg ccaaaagcgc gacgaccatc agtgcgccca
      481 aggggttgtg tccgggttgg acgtgttcgg gaataccgtt tttcatatac tctctgatac
      541 ccgaccatcc gcggacgaaa cgggagaaac gggcggtatc gctgccccaa atgccccagc
      601 agaggcggaa tacgagcagg aaaaggataa acagcccgac gcgcgtgtgc cattgcagca
      661 tatcgccgcc ggttttcgcg ctataccaca taaagggtag ggatgcggca agcagccagt
      721 ggaaaaggcg ggtggggaag tcccagactt tggttttgtt tttcataatc ggtttccggc
      781 ggtagaatcg gtttgttttc gagccttaat tttaaccgat tggaggggaa atgtttcccg
      841 ttttccatct ttcaggcgaa agccgccgcc ggatgcttca ggcggcatcg cgttttccct
      901 atgttttcaa agcccgtgcg gaagattcgc acaaagggac tttcggcacg ctcgccgtag
      961 tcggcggatc ggcagggatg agcggcgcgc ccgtattggc ggcatcggcg gcgatgtatc
     1021 tcggctgcgg caaggtgtgg gcgggtttca atcaggaaac gctgccgttt gccgtcattg
     1081 ccggttttcc cgaaattatg ttggacacgg cagacggttt gaccaagcgt caaggcataa
     1141 acgcttggat ggcaggttgc ggcctgggta cggataaagc ggcggtcgga acgcttgccg
     1201 gaattttggc ggaacacacg gacaagcccg tccttttgga tgcggacgcg ctgaacatat
     1261 tatcaaccga tgccgaaacc cgaaatctgg cgcgcgggtg taaaaacctg attttaacgc
     1321 cacaccccgc cgaagccgcg cgcctgcttg gaacgacggt tgcacaggtt caggcggatc
     1381 ggacggcggc agtgaggaag ataagggcaa ttttcggcgc aaccgtggtt ttaaaggggc
     1441 acaaaacatt ggttgcctca cccgatacgg aaatctatgt caacgaaagc ggcaacgcgg
     1501 gattggcaac ggcgggcagt ggcgacgtat tgggcggcat catcggcagt ctgctcgcac
     1561 agggcgtgcc ggtttttgaa gccgcctgcg cgggcgcgtg gctgcacggc gcggcggcgg
     1621 atgtcataaa agaatcggca ggcattgcgg cagggctgtt ggctggcgaa atcgccccgg
     1681 cggcaaggtg gctgcgcaac cggataactg aaagtatgta agaagatata gtggattaac
     1741 aaaaaccagt ac
//
LOCUS       DAWFBO010000215         1734 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWFBO010000215 DAWFBO010000000
VERSION     DAWFBO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.215"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            356..1660
                     /locus_tag="WGO87_001939"
     CDS             356..1660
                     /locus_tag="WGO87_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0393249.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          357 a          432 c          510 g          435 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cagcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgtgta tttttaagcg ggatgcagat agacgctacc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taaa
//
LOCUS       DAWFBO010000216         1708 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWFBO010000216 DAWFBO010000000
VERSION     DAWFBO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.216"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(37..699)
                     /locus_tag="WGO87_001940"
     CDS             complement(37..699)
                     /locus_tag="WGO87_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0393250.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGAGITPEHPDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLAPKLGFIIPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(712..1539)
                     /locus_tag="WGO87_001941"
     CDS             complement(712..1539)
                     /locus_tag="WGO87_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0393251.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNAPP
                     LDFPHAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          334 a          453 c          516 g          405 t
ORIGIN      
        1 atttaatcca ctatatgttt gtggggcgga tatttttcag ggctgtattt tgtccagaca
       61 ttcgagcaga tcgagcggcg tgcggatgtg gaaatccgcc tgccatgagt cggtatcgtc
      121 ttcatctgag atgtagcccc attcggcgag gacggttttc atgccggcgt tgcggcctgc
      181 ttggatgtcg cgttcggcat caccgacgta gagtgtgtgt tgcgggtcgg cgtggatttg
      241 tccgcacgca tacagcatgg gtttgacgct gggcttgggt tcgccgcagg tgtcgccgct
      301 gacgacgacg gcgggtggga tgatgaagcc gagtttgggg gcgagtttgt cggtgaagcg
      361 cataggtttg ttggtgatga tgccccattt gatgccgcgt ctgtcgagtt cggcgatgag
      421 ttcgtttacg ccgccgaaga gggtggtgtc ttgggcgtag cggctgtcgt attcgtcaag
      481 gtattcggtg cgccatcggg catagtcggg gtgttcgggg gtgatgcctg cgccgagttt
      541 gagcagtcct gccgcgccgt ggctggcttg gttgcggatt tcgtccatgc ttttgggcgg
      601 cagtccgtgg cgggcgagca gggtattgag tgcgccgccg aggtctaggg cggtgtcggc
      661 aagcgtgccg tcgaggtcga agagtacggc gtgtatcatg tgtgttcctt ttcagacggt
      721 atttttgttc tgtttcaaac cctgttcgaa atcttccaac atatccaatt cgaagcggct
      781 gaagcccgct ttttcccgcg cttcgatgtt cacatagccg cggaagataa acatatcgta
      841 acgggcaatc agactgcgga acagggcgac aggttccaaa ccgcgttcgc ggcagaggtg
      901 ttgataccaa tggttgccga tggcgacatg acccacttcg tcgcggtaaa tgatgtccaa
      961 tacgccgcag gtttccgaat cgccgcgctg ctccaccttc gcgcgtatcc ccggcgtaac
     1021 gtccagcccg cgcgcttcca acacgcgcgg taccaacgcc atacgcaaca gcgggtcgta
     1081 ggcggttttg tacgccatat cccacaaatg gttgtgcgcc tcgaaactgc cgtaatcgaa
     1141 gccgaaagcg cgcaatctgt cgcgcatcag gcggaaatgg tacacctctt ccttcgccac
     1201 tttcacccag tcgcggacaa actgaaacgg cagcgtgcgg aaacggtatg ccgcgtccaa
     1261 agccagattg acggcgttga actcgatatg cgcaatcgcg tgcagcatcg ccgcatagcc
     1321 ttctgccgtg ttcattttgc gcggggtcag ttgcgacggc gcgaccaaaa ccggcttgtc
     1381 cggccgtccc gcgtggggga aatccagcgg cggcgcgttt gtttcagccc cgtccatgtt
     1441 ctgaacggcg gcaaacgcct catccgtcag ccgtcctttt tcatctgggt cgcccgaaag
     1501 cagggcgcgt tccagcaaag cataaatatc gggtttcatc tcaagtccgc cgtgttcgga
     1561 aaacaaatat tatagcgttt aaaaaaaaca agatgaggca tataatctcc gcgattcggc
     1621 attccgcgcc caaaccgtca aatatagtgg attaacaaaa accagtacgg cgttgcctcg
     1681 ccttagctca aagagaacga ttctctaa
//
LOCUS       DAWFBO010000217         1699 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWFBO010000217 DAWFBO010000000
VERSION     DAWFBO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.217"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            41..982
                     /gene="mafA"
                     /locus_tag="WGO87_001942"
     CDS             41..982
                     /gene="mafA"
                     /locus_tag="WGO87_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0393252.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            986..>1699
                     /gene="mafB"
                     /locus_tag="WGO87_001943"
     CDS             986..>1699
                     /gene="mafB"
                     /locus_tag="WGO87_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0393253.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADGYDGPKGGNYPKPTGA
                     RDEYTYNVNGVARSIKLNPTDTRDVRQRISDNYSNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNP"
BASE COUNT          448 a          631 c          372 g          248 t
ORIGIN      
        1 atttacactc aaactcgttc aaaacaaaac aaaccctgcc atgaaaaccc tgctcctcct
       61 catccccctc gtcctaaccg cctgcggcac actgaccggc atacccgccc acggcggcgg
      121 caaacgcttt gccgtcgaac aagaactcgt cgccgcatcg tcccgcgccg ccgtcaaaga
      181 aatggatttg tccgccctga aaggacgcaa agccgccctt tacgtctccg ttatgggcga
      241 ccaaggttcg ggcaacataa gcggcggacg ctactctatc gacgcactga tacgcggtgg
      301 ctaccacaac aaccccgaaa gtgccacccg atacagctac cccgcctacg acactaccgc
      361 caccaccaaa tccgacgcgc tctccagcgt aaccacttcc acatcgcttt tgaacgcccc
      421 cgccgccgcc ctgactagaa acagcggacg caaaggcgaa cgctccgccg gactgtccgt
      481 caacggcacg ggcgactacc gcaacgaaac cctgctcgcc aacccccgcg acgtttcctt
      541 cctgaccaac ctcatccaaa ccgtcttcta cctgcgcggc atcgaagtcg taccgcccga
      601 atacgccgac accgacgtat tcgtaaccgt cgacgtattc ggcaccgtcc gcagccgcac
      661 cgaactgcac ctctacaacg ccgaaaccct taaagcccaa accaagctcg aatatttcgc
      721 cgttgaccgc gacagccgga aactgctgat tgcccctaaa accgccgcct acgaatccca
      781 ataccaagaa caatacgccc tctggatggg accttacagc gtcggcaaaa ccgtcaaagc
      841 ctcagaccgc ctgatggtcg atttctccga catcaccccc tacggtgaca caaccgccca
      901 aaaccgtccc gacttcaaac aaaacaacgg taaaaacccc gatgtcggca acgaagtcat
      961 ccgccgccgc aaaggaggat aaaccgtgaa accgctgcgc agactgacaa acctccttgc
     1021 cgcctgcgcc gtagcggccg ccgccttcgg acagcccgcc ctcgcggcgg acttggcgca
     1081 agatccgttc attaccgata acgcccaacg gcagcactac gaacccggag gcaaatacca
     1141 cctcttcggc gacccgcgcg gcagcgtttc cgaccgcacc ggtcaaatca acgtcatcca
     1201 agactatacc caccagatgg gcaacctgct catccaacag gcaaacatca acggcacaat
     1261 cggctaccac acccgctttt ccggacacgg acacgaagaa cacgccccct tcgacaacca
     1321 cgccgccgac agcgcaagca aagaacaagg caacgttgac gaaggcttta ccgtgtaccg
     1381 gctcaactgg gaaggacacg aacatcatcc cgccgacggc tatgacggcc cgaagggcgg
     1441 caattacccc aaacctacgg gagcacgcga cgaatacacc tataacgtta acggcgtagc
     1501 ccgcagcatc aaactcaatc cgaccgacac ccgcgacgtc agacaacgca tatccgacaa
     1561 ttacagcaac ctcggcagca atttctccga ccgcgccgat gaagccaaca gaaaaatgtt
     1621 cgagcacaat gccaagctcg accgctgggg caacagcatg gagtttatca acggcgtcgc
     1681 cgccggcgcg ctcaacccc
//
LOCUS       DAWFBO010000218         1652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWFBO010000218 DAWFBO010000000
VERSION     DAWFBO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.218"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            79..1248
                     /gene="metZ"
                     /locus_tag="WGO87_001944"
     CDS             79..1248
                     /gene="metZ"
                     /locus_tag="WGO87_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0393254.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1343..1549
                     /locus_tag="WGO87_001945"
     CDS             1343..1549
                     /locus_tag="WGO87_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0393255.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          404 a          430 c          435 g          383 t
ORIGIN      
        1 ggtttaaatt taatccacta taaattaaac aacgggcgca cgctccgttg ccgaacggat
       61 aaaaccgagg aatcaccgat gagcaaaaaa ctccatccgc aaacgctcgc catacgcggc
      121 ggcaaagaac aaaccgaata ccgcgaacac aatcaggcat tgttcctgac cagcagcttc
      181 atgtgggaca acgcccaaca cgctgccgat ttgttttcaa aaaaaatcaa aggcttcact
      241 tatacccgta ccgccaaccc gactacagcc gcctttgaaa aacgcatcgc cgctttggaa
      301 ggtgcggaac gcgcggtcgc cacttcgacc ggcatgtccg cgattcaggc ggcgtttttc
      361 acctttttgc aggcgggcga ccatgtgatt tccagccgca gcctgttcgg cacgaccgtc
      421 ggctttatca ataacatcgt taccaaattc ggcatcgaag tgagtcacgt gtcgccgact
      481 gatataaacg aatggaaagc cgccgtcaaa gccaacacca aactgctgtt tttggaaacg
      541 ccgtccaatc ccttgggaga agtggccgac ttggaagcct tggcggaatt ggcgcacggc
      601 atcggcgcgc tcttggtggt ggacaacagc ctgctgtcgc ctgtcggctc tcagcctttg
      661 aaacacggcg cggatatttc cgtttcctcc gcgaccaaag ccatagacgg acacgggcgc
      721 gtgatgggcg gcgtgttggc gggttcggaa gaactgatgg cgcaggtcgc cgtttactgc
      781 aactcttgcg ggctggcaat gtcgccgttt aacgcatggc agttgttgag cggcgtggaa
      841 accctgtctc tgcgtatgga aaaacagttc gacaacgcct tgaaaatcgc gcaatggctg
      901 caagcgcagc cgcaggttca agccgtgtat tacaccggat tacccgacca tcctcaggcg
      961 gagcttatcc gcaaacagca aaacggcggc ggcatcgtca tcggctttga agtcgccgac
     1021 caagcagccg cgtggaaagt cgtggacggc gtcgaacttt tctcccgtac cgccaacctc
     1081 ggcgacgtgc gctcgaccat tacccatccg tggacaacca cgcacggcag gatgcagccc
     1141 gaagaaaaac tcgccgccaa catccgcccc ggattggtgc gcctgtcggt cggtttggaa
     1201 tatgttggcg atttgattga cgatttgaaa caggcactcg cgcgctgatt gcgtgttcag
     1261 acggcatcgg cggaaaaaca ccgcaaatgc cgtctgaaac ccttatctta tatgacatac
     1321 acgtcaacaa taggaaaaaa atatggatga agcggttttt gccgattggg cgttgaaaat
     1381 ctgcctgacc ggcttgatta tttttttggg ttttatcgtt tggaatttgg gcaaggagtc
     1441 taaagcgggc aaattcggca tcgccatttt gtttttggtt ttgggcttgg gcgtgtttgg
     1501 ttttgtgttc aaagagttat tgattaagtt tttggtattg ccgaaatagg tttgcggtct
     1561 gtcaaaatgc cgtctgaaat cataaaaagt aatctgcacc ctaaaagttt gatttaaccg
     1621 ccaactgatt aaggtgcgga tttttatgac aa
//
LOCUS       DAWFBO010000219         1649 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWFBO010000219 DAWFBO010000000
VERSION     DAWFBO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.219"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            295..1455
                     /locus_tag="WGO87_001946"
     CDS             295..1455
                     /locus_tag="WGO87_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0393256.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     QNIQQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGATRW
                     GNRESFVGLAGEFGTLRVGRVANQFDDASKAIDPWDSNNVVASQLGIFKRHDDMPVSV
                     RYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQQTPQIQPTLVPAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKG
                     ENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          408 a          392 c          424 g          425 t
ORIGIN      
        1 gtgccgtgtg tgttttttat ggcgttttaa aaagccggga ctgcatccgg gcagcagcgc
       61 atcggcccgc acgaggtctg cgcttgaatt gtgttgtaga aacacaacgt tttttgaaaa
      121 ataagctatt gttttatatc aaaatataat catttttaaa ataaaggttg cggcatttat
      181 cagatatttg ttctgaaaaa tggttttttg cggggggggg ggtataattg atgacatatc
      241 gggtgtttgc ccgatgtttt taggttttta tcaaatttac aaaaggaagc cgatatgcga
      301 aaaaaactta ccgcgctcgt attgtccgca ctgccgcttg cggccgttgc cgatgttagc
      361 ctgtacggcg aaatcaaagc cggcgtggaa ggcaggaaca tccagctgca gttgaccgaa
      421 ccgctccaaa atattcaaca acctcaggtt actaagcgca aaagccgcat caggacgaaa
      481 atcagcgatt tcggctcgtt tatcggcttt aaggggagcg aggatttggg cgaagggctg
      541 aaggctgttt ggcagcttga gcaagacgta tccgttgccg gcggcggcgc gacccgttgg
      601 ggcaacaggg aatcctttgt cggcttggca ggtgaattcg gcacgctgcg cgtcggccgc
      661 gttgcgaatc agtttgacga tgccagcaaa gccattgatc cttgggacag caataatgtt
      721 gtggcttcgc aattgggtat tttcaaacgc cacgacgata tgccggtttc cgtacgctac
      781 gattcccccg aattttccgg tttcagcggc agcgtccaat tcgttccggc tcaaaacagc
      841 aagtccgcct atacgccggc tcattttgtt cagcagactc ctcaaattca gcctactctc
      901 gttccggctg ttgtcggcaa gccggggtcg gatgtgtatt atgccggtct gaattacaaa
      961 aatggcggtt ttgccgggaa ctatgccttt aaatacgcga aacacgccaa tgtgggccgt
     1021 gatgcttttg agttgttctt gctcggcagc gggagtgatg aagccaaagg taccgatccc
     1081 ttgaaaaacc atcaggtaca ccgcctgacg ggcggctatg aggaaggcgg cttgaatctc
     1141 gccttggcgg ctcagttgga tttgtctgaa aatgccgaca aaaccaaaaa cagtacgacc
     1201 gaaattgccg cgactgcttc ctaccgcttc ggtaatgcag ttccacgcat cagctatgcc
     1261 catggtttcg actttatcga acgcggtaaa aaaggcgaaa ataccagcta cgatcaaatc
     1321 atcgccggcg ttgattatga tttttccaaa cgcacttccg ccatcgtgtc tggcgcttgg
     1381 ctgaaacgca ataccggcat cggcaactac actcaaatta atgccgcctc cgtcggtttg
     1441 cgccacaaat tctaaatatc ggggcggtga agcggatagc tttgtttttg acggctcgcc
     1501 ttcattcttt gattgcaatc tgactgccaa tctgcttcag ccccaaacaa aaatccggat
     1561 acggaagaaa aacggcaata aagacagcaa ataccgtctg aaagattttc agacggtatt
     1621 tcgcattttc ggcttggttt gcacatata
//
LOCUS       DAWFBO010000220         1644 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWFBO010000220 DAWFBO010000000
VERSION     DAWFBO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.220"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(282..1274)
                     /gene="porB"
                     /locus_tag="WGO87_001947"
     CDS             complement(282..1274)
                     /gene="porB"
                     /locus_tag="WGO87_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ0393257.1"
                     /translation="MKKSLIALTLAALPIAAMADVTLYGTIKTGVETSRSVYYEDGKA
                     AEVTTGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHEQIDNVKIEKYQIHRLVSG
                     YDNDALHASVAVQQQDAKLVEDNYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFD
                     DADRYNTYDQVVVGAEYDFSKRTSALVSAGWLQEGEGESKFVSTAGGVGLRHKF"
BASE COUNT          447 a          365 c          406 g          426 t
ORIGIN      
        1 agtttcaaaa tttattctaa atagctgaaa ctcaacgcac tagattcccg cctgcgcggg
       61 aatgacggca tattttgaca ttgaataaaa aagactaaaa acaggaaaaa gccaaacaga
      121 aaacagcgaa acagaaaaaa gccaaacaga aaaaagccaa acagaaaaaa gccaaacaga
      181 aaacagccaa acagaaaaca gccaaacaga aaacaggaaa aagccaaaca gaaaaaagcc
      241 tgtctggcga caggcttttt gttgatacca atctttgcag attagaattt gtggcgcaga
      301 ccgacaccgc cggcagtcga tacgaatttg ctttcgcctt cgccttcttg caaccaaccg
      361 gcagaaacca aggcagaagt gcgtttggag aagtcgtatt ccgcaccgac aaccacttgg
      421 tcgtaagtat tgtagcggtc tgcatcatca aacgagcctt tgaagccgtg ggcgtaagaa
      481 acgcggggcg ttacgttgcc gaagcggtat gccaaggtag cggcaacttc ggtttgagag
      541 ttgtgcgaat aattgtcttc aaccaatttg gcatcttgtt gttgtacggc tacggaagcg
      601 tgcagggcat cattgtcgta accgctgacc aaacggtgaa tctggtattt ctcaatcttc
      661 acgttatcta tctgctcatg tcttttatag gcaccgccat attgcacgaa gaagccgccg
      721 tttttgtagt tgaagccggc gtggtaagat tcgctgttat gtctgcctgc attgtcgtta
      781 agcgcgtatt gtacgctgcc gctgaggccg gcaaattcgg gagaatcgta gcgtacggaa
      841 atgaggcgtg cctcgggttc ggcaattttg tttacaccca aatagtcgct tttgctatcc
      901 caaggattga tgtcgccggt atctttcagg acgctgttca gacggccgac gcgcaattta
      961 ccgaagccgc ctttcaaacc gatgaaggat tggcggttgc cccaaccgga gtcagtaccg
     1021 gcgatagatg ccttttgctc aacctgccaa atggctttca gaccgttacc gaggtcttct
     1081 tggcctttga agccgatttt cgaacccaaa tcaacgatgc cggtaccggt tgtaacttca
     1141 gcagctttgc cgtcttcgta atatacagag cgggaagttt ctacgccggt tttgatggtg
     1201 ccgtacaggg taacgtcagc cattgctgca ataggaaggg ctgccaaagt cagggcaatc
     1261 agggattttt tcattgctgt attccttttt tggttaagaa atttaagcag gccgggcttt
     1321 ccaagccgct tagctttgca tttaccgccg acgttttcgg cgattccatg tctgaaacta
     1381 tagatgttcc tgtctgaaaa aacaaacttc tgcctgtttt tgtgtggaat tcgatgtgta
     1441 ttttgaaggg cggatagaga tattgtgggt attttttgtt ttataacata tggttattta
     1501 aattttttaa gatttgcatt tttacaacac ttactcggga gggtattgga gggcattgca
     1561 aaccgagggt tataaagacg gcaaaaaacg cgcacgtggt ttctttcgga acgggcggat
     1621 gcaaaacccg cccctgcggc agga
//
LOCUS       DAWFBO010000221         1591 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWFBO010000221 DAWFBO010000000
VERSION     DAWFBO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.221"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            59..1432
                     /locus_tag="WGO87_001948"
     CDS             59..1432
                     /locus_tag="WGO87_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0393258.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          414 a          522 c          384 g          271 t
ORIGIN      
        1 cccatcctat cggtttcccg cttcagacgg cataaggtct gaaagaaaga ctacaattat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttacta gccgccgcgc aaaccggcac gggcaaaacc gccgccttta tgctgccctc
      241 gctcgaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtg ttgaccccca cgcgcgaact cgccgaccaa atcgaccaaa acgtgcagtc
      361 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      421 acagaccgcc gatttgcgtg ccggctgcga aatcgtcgtc gccacagtcg gacggctgct
      481 cgaccacgtc aaacaaaaaa acatcagttt gaacaaagta gaaattgtcg ttttggatga
      541 agccgaccgt atgctggata tgggttttat cgacgacatc cgcaaaatca tgcagatgct
      601 gccccgccaa cgccaaaccc tgctcttttc cgccaccttc tccgccccga tacgcaaact
      661 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      721 cgccaacgtc gaacaacaca tcatcgccgt cgataccttc caaaagcgca acctgctcga
      781 acggctgatt gtcgatctgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      841 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcagcctgcg
      961 cgtcctcgtc gccaccgaca tcgccgcgcg cgggctggac attgccgaac tgcccttcgt
     1021 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 tgaatccatt aaagagctga ccggcaacaa gctgctcatt gagcgcatcg agggcttcga
     1201 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa cccgaaatgc gcgaaccgag
     1261 acaacgcaac cgctacgaat ccgccaaagc gcaacgcgaa aaaaacaccc ggccggaaac
     1321 cacggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1381 ccgggaacac cggacctgcg ccctgctcca accgcgttac ggcgtaaaat agccctgaaa
     1441 atcaaatgcc gtctgaacat ttccgtttca gacggcattt ttcaaaccgg actgacgcat
     1501 tgggcgcaac cgcccgcacc ggataaattt ctgccgcaaa cagtttcaga cggcatttgc
     1561 tgcctgtaca atatagtgga ttaacaaaaa t
//
LOCUS       DAWFBO010000222         1542 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWFBO010000222 DAWFBO010000000
VERSION     DAWFBO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.222"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            96..404
                     /locus_tag="WGO87_001949"
     CDS             96..404
                     /locus_tag="WGO87_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393259.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFTF
                     DVVQAQALAQAIMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            486..1517
                     /gene="ruvB"
                     /locus_tag="WGO87_001950"
     CDS             486..1517
                     /gene="ruvB"
                     /locus_tag="WGO87_001950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0393260.1"
                     /translation="MLQTDNLTAAQPQRIITAQSISSQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAILHKFSGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
BASE COUNT          385 a          518 c          354 g          285 t
ORIGIN      
        1 agttttgagg aggataccca atccaagctt tgcaacagcc gccgccatat ccgctataat
       61 tcacgcttca gccatcctgc ccccgacata aaatcatgac cctgaaaacc gatttattgc
      121 ctaaaatcaa caacgaagat tatcaacgcc tcatcctcaa acacagtgcg gaatttagcg
      181 gtggcgaaat ccgcctgttg aacgaaatcc ttgaaaaatt caccttcgac gtcgttcaag
      241 cgcaggcatt ggcgcaggcg ataatgcagc aagtccgctt cgaccccaac gcctatcaca
      301 tcgacagcga tgacgaagac accacaggca tctgcccaca ctgcatcaat ccgcccatgc
      361 cgcccctgcg cgattacctc gtttggcgcg aaacgcgcgg ataaaacgct tttgaccgtt
      421 atcttttcaa tgccgtctga aacgccgccg accgttcaga cggcataccc gacaaaggga
      481 acactatgct gcaaaccgac aacctaaccg ctgcccagcc ccagcgcatc atcactgccc
      541 aaagcatctc ctcacaagaa gagttgctcg aacgtgccct gcgtcctaaa acgctggacg
      601 actatatcgg gcaagacaaa gccaaagaac agcttgccat tttcatccaa gccgccaaaa
      661 aacgcggcga agcactcgac cacgttttgc tcttcggccc gcccggactg ggcaaaacca
      721 cactggcgca catcatcgcc aaagagctgg gcgtcaacct gcgccaaacc agcggccccg
      781 tcctcgaacg cgccggcgac cttgccgccc ttctgaccaa ccttgatccg cacgatgtat
      841 tgttcatcga cgaaatccac cgcctcagcc ctgttgtcga agaaatcctc tatcccgcgc
      901 tcgaagacta ccggctcgac attatgatag gcgaaggacc cgccgcccgt tccgtcaaaa
      961 tcgacctgcc gcccttcacc ctcgtcggcg cgaccacccg cgccggtatg ctgaccaatc
     1021 cgttgcgcga ccgcttcggc atcgtctccc gccttgagtt ttacgaaaac cgagacctta
     1081 ccaccatcgt cagccgttcg gcacaactgt tgcagctcga tatgtccgaa gaaggcgcgg
     1141 aagaaatcgc caaacgcagc cgcggtacgc cgcgcatcgc caaccgcctg ttgcgacgcg
     1201 tgcgcgattt cgccgacgtg aaaaacaacg gcgtaatcga tgccgccgtc gccgatgccg
     1261 ctttaagcat gctggatgtg gacgcgcaag gtttggatgt tatggacaga aaattcctcg
     1321 aagccatcct gcataaattc agcggcggcc ccgtcggttt ggacaacgtt gccgccgcca
     1381 tcggcgaatc tacagacacc atcgaagacg ttatcgaacc ctatctcatc caacaaggct
     1441 tcctgcaacg caccccgcgc ggcaggatgg cgaccgaacg cgcctacctg catttcgggc
     1501 tgcccgtcga aaaataacgc aatgccgtct gaaacagagc ta
//
LOCUS       DAWFBO010000223         1445 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWFBO010000223 DAWFBO010000000
VERSION     DAWFBO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.223"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            27..1364
                     /locus_tag="WGO87_001951"
     CDS             27..1364
                     /locus_tag="WGO87_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0393261.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGQNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGIWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          259 a          347 c          391 g          448 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacaggc ggtcaaaacg
      241 ccgtcgattc cttcagggct ctgcgtccga gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgtttccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattaa
      361 attatgtcgt ccacagtttc acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtctg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggc caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcagtgt
      901 ttatgaagat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcgttt tcgatgttgg aaacggtcat tgcctcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa cacacttggc ttatcggcac ggctattttc attgtcggca
     1081 tcccgtccgc gctgtctttc ggcatctggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactatgtt atttccgccg tcattatgcc tgtcggcgcg ttgggcgttg
     1201 ccgtatttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac ctcgccccga
     1321 ttgccattat tattgttttc gtcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaaa
//
LOCUS       DAWFBO010000224         1431 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWFBO010000224 DAWFBO010000000
VERSION     DAWFBO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.224"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            1..106
                     /locus_tag="WGO87_001952"
                     /pseudo
     CDS             1..106
                     /locus_tag="WGO87_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
     gene            302..649
                     /locus_tag="WGO87_001953"
     CDS             302..649
                     /locus_tag="WGO87_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0393262.1"
                     /translation="MTGFNDAAGVASASDIKGKYVKSVEVKNGVVTATMASSNVNNEI
                     KGKKLSLWAKRQDGSVKWFCGQPVTRKANNADDVAADGNDKIDTKHLPSTCRDESTAG
                     CIETPPTAFYKNT"
     gene            1290..>1431
                     /gene="pilE"
                     /locus_tag="WGO87_001954"
     CDS             1290..>1431
                     /gene="pilE"
                     /locus_tag="WGO87_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HEZ0393263.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLA"
BASE COUNT          452 a          419 c          269 g          291 t
ORIGIN      
        1 gcgcaccgac gccaaagccg acaccgtcgc cgccgccgcc aagaccgccg acaacatcaa
       61 caccaagcac ctgccgtcaa cctgccgcga cgcttcatct gccagccgta caaaaacacc
      121 cggtttcgct ttcaaatact tccaaaaaat cagccgttac cgagtattgc ccgaatcacg
      181 gcaaatgact aaaaaacttc gtcattcccg cgcaggcggg aatctaggtt tgtcggcgca
      241 gaaacttatc gggtaaaaag gtttctccag tcctgaatcc tagattccca ctttcgtggg
      301 aatgacggga tttaatgatg ccgccggcgt ggcatccgcc tccgacatca aaggcaaata
      361 tgttaaaagc gttgaagtca aaaacggcgt cgttaccgcc acaatggctt caagcaacgt
      421 aaacaacgaa atcaaaggca aaaaactctc cctgtgggcc aagcgtcaag acggttcggt
      481 aaaatggttc tgcggacagc cggttacgcg caaagccaac aacgccgacg acgtcgccgc
      541 cgacggcaac gacaaaatcg acaccaagca cctgccgtca acctgccgcg atgaatcaac
      601 tgccggttgc atagaaacac cacctacggc tttctataaa aatacctaaa ccgtcattcc
      661 cacgaaccta catcccgtca ttcccgcgaa agtgggaatc cagaacgtaa aatctaaaga
      721 aaccgttttc ccgataagtt tccgtgccga cgaggctgga ttcccgcctg cgcgggaatg
      781 acgaagccat ccgcacggaa acctgccgcg atgattcaac tgccgtttgc acaggaacac
      841 cacgcgccga tttcaatcac tttccaagaa aacggaaatt tttaaaaatt aaatttaaaa
      901 tttcccaccc aacccaccct atgctaccgc gcaaattcaa aaatctcaaa ttccgaccca
      961 atcaacacac ccgatgcccc atgccaatag aaatacccca cgccaataaa aaagtgccga
     1021 aaatcggcac taaaactgac aattttcaac actgatgaca aaaaacggta accccctgct
     1081 tcctcaaacc acgcccaaac gaaagaaaat gtaaaataaa aacaaccata taaagataaa
     1141 cgcataaatt ttcacctcaa aacacaaact tgataccaat cttgctttat aatacacagg
     1201 tatcgcaaca aaataccgat ggtcaaatac attgcataat gccgatggcg taagcttgaa
     1261 gcatttcctt tccaattagg agtaatttta tgaacaccct tcaaaaaggt tttaccctta
     1321 tcgagctgat gattgtgatt gccatcgtcg gcattttggc ggcagtcgcc cttcctgctt
     1381 atcaagacta cacagcccgc gcacaagttt ccgaagccat tcttttggcc g
//
LOCUS       DAWFBO010000225         1416 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWFBO010000225 DAWFBO010000000
VERSION     DAWFBO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.225"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="WGO87_001955"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="WGO87_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0393264.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT          379 a          409 c          308 g          320 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggcccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgattgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtttgg gtatgtggga caaaattccc gaaaacgaaa tctacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 cgaggcgcgc ggcgaacctg ccgactgttt gggctatctt atttccaacc acaatatccg
      541 acgcgctgcc tacgaagtcg tatcccaact cgacaacatt accattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgcttc tcgcaaaccc gtcgacaact
      721 cggtatctcc tccgatatgc acgattacag ccgaaccatg ttcgtctgcc gtatgaaaca
      781 taccctttcc aaccaacata ccgcttacga atgcttccat tacggacgca ccatcgcact
      841 gctgccgctg gaagagcacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc gtaaaagaac agctcaaggg
      961 caggcttggc gacatggaac tggtcagcag tatccaccat tacccccttg tcggtatgat
     1021 tgccaaacgt ttctacggta aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc atgcccagcc gctttatcac ggaaccaata tgatgctgaa
     1261 actgtttacc aatgagaccg cgccggcaaa actgctgcgc ggtttggtat tgcgtgccgg
     1321 caacaacttc ccgccgctga aaaaactgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttg
//
LOCUS       DAWFBO010000226         1375 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWFBO010000226 DAWFBO010000000
VERSION     DAWFBO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.226"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..761
                     /gene="mafB"
                     /locus_tag="WGO87_001956"
     CDS             <1..761
                     /gene="mafB"
                     /locus_tag="WGO87_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0393265.1"
                     /translation="GIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEK
                     NTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDFSAAYNTRI
                     TRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHL
                     FPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKYIAKGRPARWVSYET
                     RDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            776..1006
                     /locus_tag="WGO87_001957"
     CDS             776..1006
                     /locus_tag="WGO87_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0393266.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1155..>1375
                     /locus_tag="WGO87_001958"
     CDS             1155..>1375
                     /locus_tag="WGO87_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-containing protein"
                     /protein_id="HEZ0393267.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTAQYGNPYR
                     ETVYIEISDGIGNNQTLISNKIHPFYSQG"
BASE COUNT          480 a          295 c          284 g          316 t
ORIGIN      
        1 tgggcatagg cgacatactg tacggaacgc gctatgccat agacaaagcc gcaatgcgca
       61 acatcgcccc cttgcccgcc gagggcaaat tcgccgtcat cggcggcttg ggcagcgtgg
      121 cgggctttga aaaaaatacg cgcgaagccg ttgaccggtg gatacaggaa aaccccaatg
      181 ccgccgaaac cgtcgaagcc ctggtcaacg tcctgccgtt tgccaaagtc aaaaacctga
      241 caaaggcggc aaaaccgggg aaagctgcgg ttagcgggga tttttctgct gcatacaata
      301 caagaataac tagaaaagtt actacagaaa cagaggggtt aaatagaatc agacagaacc
      361 agaaaaatag taatatacat gagaaaaatt atggaagaga taatcctaat catattaatg
      421 ttttatctgg aaattctata caacatatac tgtatggaga tgaagcagga ggtgggcatc
      481 tttttcctgg caaacctggt aagacaacat tcccccaaca ttggtcagcc agtaaaataa
      541 ctcatgaaat tagtgatatc gttacatccc caaaaacgca atggtatgca cagactggaa
      601 caggcggcaa atatattgct aaaggaagac cagccaggtg ggtatcatat gaaacgagag
      661 atggaattcg tatcagaaca gtttatgaac ctgcaacagg aaaagtggta actgcattcc
      721 ccgatagaac ctctaatccc aaatataacc ctgtaaaata atgagtattt gaaagatgaa
      781 aacattagac gaacgaataa aaaatttagg aaaatctctt gaagatagaa ttgacgctaa
      841 tctaattgat gccgcactag agtacattac tttttcagaa cgtttgctag cttttgaaac
      901 gctatgtgat tatatagaag atttcaatat ccagcttacg gaaaaagaat cccaagaaat
      961 ctcttttatt aataaggaat ttggaattga aagtacatca gattaataaa tattaggtcg
     1021 gtttaaaaac ccaacaatca aaaagtcgtc tgaaactgtg tttaatttac agacgacctt
     1081 tccttcattt gaagcaggat attgagaact gagttcttca aaaatcctac acctgctcct
     1141 tccacggcag caccttggtc aaaacggcag acggctacaa agccattgcc catattcaag
     1201 ccggcgaccg cgtcttcgcc aaagatgaaa ccagtggaaa aactggatac aaacctgtta
     1261 ccgcccaata cggcaatccg tatcgggaaa ccgtttacat tgaaatttca gacggcatcg
     1321 gcaacaacca aaccctgatt tccaataaaa tccacccgtt ttacagtcaa ggaaa
//
LOCUS       DAWFBO010000227         1309 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWFBO010000227 DAWFBO010000000
VERSION     DAWFBO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.227"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(99..1022)
                     /locus_tag="WGO87_001959"
     CDS             complement(99..1022)
                     /locus_tag="WGO87_001959"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0393268.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          350 a          312 c          330 g          317 t
ORIGIN      
        1 gggaatgacg aattttaggt ttctgttttg gttttttgtc cttgtaggaa tgatgaaaat
       61 ttaagtttta ggaatttacc ggaaaaaaat agaaagcgtt atccacaagt tctgatgttc
      121 agctcgtgaa atgcgtcggg caaatcatcg ctgtcggcaa attccacccg gtcgtaagcc
      181 gtttcgtctg ccaaaaccgc gcgcaagagt gcgttgttga tggcgtgtcc cgatttgtag
      241 ccttcaaatg cgccgacaat cggatgtccg acgatataca aatcaccgat ggcatcaagg
      301 attttgtggc gcacaaactc atcgggatag cgcaagcctt caggattcag gacatccgtg
      361 tcgtcaatca cgatggcgtt gttcaaattg ccgcccaaac ccagattgtg ggcgcgcatc
      421 atttccactt cgtgcataaa gccgaaagtg cgcgcgcgcg cgatttcgtc gatgtaggat
      481 ttgccggcga aatcgatttc aaaagtgggc gagctgcggt tgaaaaccgg atggtcgaat
      541 tcgatggtca gcgttacctt aaagccgtca tacggcgtaa agcgcaccca tttgcccgct
      601 tctttgattt cgacaggctt gaggattttc aaaaaacgct tttgcgcctt ttgatcgacc
      661 acgcccgcat cttgcaaaag gtaaataaac ggcaggctgg agccgtccat aatcgggatt
      721 tcgggcgcgt tcagctcaat cagcgcgttg tcgataccgt aggcggacag cgcggacata
      781 atgtgttcga tcgtgccgac gcgcacgcct ttgtcggtaa cgatggtgga ggaaaggcgg
      841 gtatcgttga tcaaataagg ggtcagtttg atttgttcgc ccatctcgcc gtccaaatcg
      901 gtacggcgga aggaaatccc gctgttttca ggcgcggggt gcagggtcag tgcgacccgt
      961 tcgccggaat gcagcccgac tccggtaacg ctgatggatt tcgccaaagt tctttgcagc
     1021 ataaaccgct tccttatcaa tggggtaagt tttggaataa tacgataaaa ccggaaaaac
     1081 aggctatgtt tttccatagt atttgccaat gtatccgttt tcaatacgta agccgcataa
     1141 aaatgtatag tggattaaca aaaatcaaga caaggcgacg aacccgcccc tcccgaaaaa
     1201 cgcaaaaaat gccgtccgaa aacctttcgg acggcatttt cgcgtgaacc gtcattccca
     1261 caaggacaaa aaaccaaaac agaaaaccaa aaacagcaac ctaaaattc
//
LOCUS       DAWFBO010000228         1253 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWFBO010000228 DAWFBO010000000
VERSION     DAWFBO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.228"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            236..1189
                     /locus_tag="WGO87_001960"
     CDS             236..1189
                     /locus_tag="WGO87_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0393269.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          200 a          271 c          432 g          350 t
ORIGIN      
        1 aaaccggatg attcgggcta aataattcct gattctttgg atatagccaa cggtatgcct
       61 gccgatgccg tcagaaaggg cttgatgggc gtttgcggtt cgggttaata tttgtgctgt
      121 atctgttaat tccggttagt aaaatggggc ggggacgatg agtggccgtc ggtcggtttt
      181 gcacgggcgg taataatgtc cggcgcaagg tgcgccgttt cgggaggggt gtgtgatggg
      241 ttttctgatt ggtttgagcc gcaacctgac gcggtttacg gctttggtca ttttgcttgc
      301 gtcggcggtt gcgtttatcg gaccggcttc gtttgcgtgg gttaagggcg atgttcaggt
      361 cttggtgctg ggcatcatta tgctgggtat ggggatgact ttgggcaggg aggactaccg
      421 gattttggcg cagcgtcctt tggatgtgtt tatcggtgcg gcggcgcaat ataccgtgat
      481 gccgttgctc gccatcggta ttgccaagct gttcgacctt tcgccggggc tgactttggg
      541 gctggtgttg gtggggacgt gtccgggcgg cgtgtcgtcc aacattatga gtttccttgc
      601 caaaggcgat gtggcgtttt cagtcggtat gacgacggtg tctacggtgc tggctccggc
      661 cgttacgcct ttgtggatga cctgcctggt cggtcagacg gtggatatgg acggttgggg
      721 gatgttccgg tttatgctgc tggtcacgct tttgccggtg gtgctgggtt cggcggcaaa
      781 tatcctgctg cacggaaaac attggtttga aaatgtccgt gccgttatgc cgggcgtggc
      841 ggtattggca tttgcctgca ttgtcggcgg tgtggcttcg gttcacggac accgttttgc
      901 cgaatcggcg ttggtaatgg tgttggcgat tgcggtacac aatatcgccg gctatgtgtc
      961 aggctattac gccgccgcgc tgtttggtat ggatacggca aaaaaacgca ccttggcaat
     1021 cgaagtcggc gtgcagaatg ccggtttggc aacaggattg agcgcgaaat ttttcccagg
     1081 caaccccgaa tcggcggttg cggcggcggt cgcctgcgtg tggcattcgg tttcggggac
     1141 ggtattgggc aacttgttcg ctctgtggga caagcgtcgg gacgcttagt cgggcgtgtc
     1201 cgaaccgtcc cacgatatat agtggattaa ctttaaaccg gtacggcgtt gcc
//
LOCUS       DAWFBO010000229         1229 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWFBO010000229 DAWFBO010000000
VERSION     DAWFBO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.229"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            37..369
                     /locus_tag="WGO87_001961"
     CDS             37..369
                     /locus_tag="WGO87_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0393270.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            701..1210
                     /locus_tag="WGO87_001962"
     CDS             701..1210
                     /locus_tag="WGO87_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0393271.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          327 a          229 c          295 g          378 t
ORIGIN      
        1 tgttttgacg gaaaaccatc atataaagga acacttatgc agattttatc ttttcagccg
       61 gacattgcgg aacgtatgct ggaaggtacg gaaggcgagt cggtcaacga aaacgcacaa
      121 ttcgtccgta cggacaacgg ctattggatt gcgtggcatg aaggcgtagc ggcactgctt
      181 gcgcccgata cgccgccggg cattccctgt ttttgggtgg aaggcgcgga aagccttgaa
      241 gagttgtgcg tcatggtgga acgcggcgag tttgacgaag tggaagagtt tgacggcgat
      301 gacgacgaat ggctcgaaac ggcacagggt tgcgggcacc acggcgacgc ttgcgcctgc
      361 ggacattaaa ggcattgcag gcttgccgca aggggggcaa ggctttgccg ttttttaaat
      421 aatcggtgga ttgcatctgt ttgcaatggg ctgaacgctt ccctttgtat tttattgaat
      481 aaaaaaactt atcttggtat tataattaag gcagcatcaa ttattttggg atggcaataa
      541 acgcaaagca ttgatttgcg ccgattgcag acttattata gcaggttgcg gcgcggactt
      601 aatgggaatt gtttatagag catgatggtc tgaaacatca ttaattaaaa ataccattaa
      661 tccgatttat atttatttca atttcaatgg aaaaacatca atgacaatga ttttaagcat
      721 tttaagcctg ttttttatca tcagactgtt atttttagcc gtctctatta aacatgaaaa
      781 agccttgatt gccaaagggg cgaaacaata cggaaaaacc aattccacgc tgcttgcggc
      841 agttcatacg ctttattatt tggcgtgttt tgtttgggta tggctttctg acactgcttt
      901 taatggcata tccttgattg gtacgctgac ggtgatggct tcgtttgtga tattgtcatt
      961 gattattaag cagttggggg agatttggac ggttaaaatc tatattttac caaatcatca
     1021 aattaatcgt tcgtggttgt ttaaaacatt ccgccacccc aattattttt taaacatcat
     1081 acccgaactg attggcatcg ccttattatg tcaagcgtgg tatgttttat tgattggtct
     1141 gcccatttat ttgctggtct tatttaagcg tatccgacaa gaagaacagg cgatggcaac
     1201 acttttttaa cccgtttcat caattatag
//
LOCUS       DAWFBO010000230         1208 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWFBO010000230 DAWFBO010000000
VERSION     DAWFBO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.230"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>1208
                     /locus_tag="WGO87_001963"
     CDS             <1..>1208
                     /locus_tag="WGO87_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0393272.1"
                     /translation="FAKHGYAALAELDSNGDNIINAADAAFQTLRVWQDLNQDGISQA
                     NELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKMGDLLLAADNLH
                     SRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQLALL
                     DNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKA
                     AIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAKNIYQNLLFQTR
                     LQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEG
                     RRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHNKNVSLY
                     GNDGNDTLIGG"
BASE COUNT          373 a          275 c          272 g          288 t
ORIGIN      
        1 tttgccaaac acggctacgc ggctttggcc gaattggatt caaacggcga caacatcatc
       61 aacgcggcag acgccgcatt ccaaaccctg cgtgtatggc aggatctcaa tcaggacggc
      121 atttcccaag ctaatgaatt gcgtaccctt gaagaattgg gtatccaatc tttggatctc
      181 gcctataaag atgtaaataa aaatctcggt aacggtaaca ctttggctca gcaaggcagc
      241 tataccaaaa cagacggtac aaccgcaaaa atgggggatt tacttttagc agccgacaat
      301 ctgcacagcc gcttcaaaga caaagtggaa ctcactgccg aacaggcaaa agccgccaat
      361 cttgcgggca ttggtcgtct gcgcgatttg cgcgaagctg ccgcattgtc cggcgatttg
      421 gccaatatgc tgaaagctta ttctgccgcc gaaactaaag aagcacagtt ggcattgtta
      481 gataatttga ttcacaaatg ggcggaaacc gattcgaact ggggcaaaaa atcgccaatg
      541 cgactttcaa ccgattggac gcaaacggct aatgaaggta ttgcactgac accatcccaa
      601 gtagcacaac taaaaaagaa cgctttagtt tccctttctg ataaagctaa agcagctatt
      661 gacgccgccc gcgaccgcat tgccgtgctt gatgcctaca cggggcagga ttccagcaca
      721 ctctattaca tgagcgaaga agacgcgctt aatatcgtca aagtaaccaa cgatacatac
      781 gaccatctcg ccaaaaacat ctaccaaaac ctgttattcc aaacccgttt gcagccatat
      841 ttgaatcaaa tcagtttcaa aatggaaaat gatacgttca ctttggattt tagtggtctt
      901 gttcaagcat ttaaccatgt caaagaaact aatccgcaaa aagcttttgt ggatttggcc
      961 gagatgcttg catatggcga acttcgttct tggtatgaag gccgaagact aatggccgat
     1021 tatgtggagg aggcaaaaaa agcaggtaaa tttgaagatt accagaaagt gttgggtcag
     1081 gagaccgttg cattattagc taaaacatcg ggtacgcaag cagatgatat cctgcaaaat
     1141 gtaggctttg gtcataataa aaatgtttct ttatatggta atgacggtaa cgacactcta
     1201 atcggcgg
//
LOCUS       DAWFBO010000231         1207 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWFBO010000231 DAWFBO010000000
VERSION     DAWFBO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.231"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..533
                     /gene="mafB"
                     /locus_tag="WGO87_001964"
     CDS             <1..533
                     /gene="mafB"
                     /locus_tag="WGO87_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0393273.1"
                     /translation="DQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDF
                     SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKST
                     LNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKN
                     GVHIVPANPKTIKKVQ"
     gene            536..853
                     /locus_tag="WGO87_001965"
     CDS             536..853
                     /locus_tag="WGO87_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393274.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEERRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            954..1207
                     /locus_tag="WGO87_001966"
                     /pseudo
     CDS             954..1207
                     /locus_tag="WGO87_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
BASE COUNT          406 a          236 c          254 g          311 t
ORIGIN      
        1 ttgaccagtg gatgcaggaa aaccccaatg ccgccgaaac cgtcgaagcc ctggtcaacg
       61 tcctgccgtt tgccaaagtc aaaaacctga caaaggcggc aaaaccgggg aaggctgcgg
      121 ttagtgggga tttctcagac tcctacaagc ataacactgc ttcaagatta tctcagtctg
      181 tagatggaga aatgtttcaa acccgcaatg ttgattttaa agcaaaatct attgggacta
      241 aaattcatga tggagctcaa gggaaacata tttcaggaca tagaaactac attgaaggta
      301 agagtacttt aaatcaaaac attaatcctc aagaattgtt gaacggaata cattcaggtg
      361 cttatccagt tatttctaaa ggagcaagag gaaatcctgt tgttgatttt gggtatccta
      421 taggcagcga tgggaaatca ggattaagta ccaattttgg tacgattcat tcaggtaaaa
      481 atggagttca cattgttccg gctaacccta aaaccattaa aaaggtgcaa tagttatgaa
      541 tatattacca agctggctgc gagtcggtat gaatatagca atgctgggca tgatacactc
      601 agatatcagg ttaattaccg tagattacga ggaaagaaga aggtttttaa aaatcaaaaa
      661 ttatttatca agagaagcca tcacagaaga ccatgaagat atggaatatt tgattacaga
      721 gttatggtct atgtgtggag aatattttga tgaagctgac tttgaatgta tttattctaa
      781 tcattcttct atggagttaa accaaataaa tggtgcagta ttcaggagaa aggaattaat
      841 ttcgcaagcg taggttaaaa aaccaacaat cacaatgtct tctgaaaccg tgtttaattt
      901 tcagacggca tttccttcat ttgaaatagg atattgagaa ctgagttctt caaaaatcct
      961 acacctgctc cttccacggc agcaccttgg tcaaaacggc agacggctac aaagccattg
     1021 cccgtatccg aaccggcgac cgcgtcttcg ccaaggacga ggcaagcgga aaaacgggat
     1081 acaaacccgt taccgcccga tacggcaatc cgtatcaaga aaccgtttac attgaaattt
     1141 cagacggcat cggtaaaata cagaccctcg tttccaataa aatccacccg ttttacagtc
     1201 aaggaaa
//
LOCUS       DAWFBO010000232         1176 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWFBO010000232 DAWFBO010000000
VERSION     DAWFBO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.232"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          369 a          329 c          243 g          235 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
       61 ctatatccgt ttgcgcccgc caagccgtgt tgcacagatg cgacacggct tttttctttc
      121 tgttgtcaga atgtaaattt tcgtcaaaaa acatcaaaaa tcaccccccc cccgaatttg
      181 cgtaaaataa agtgtaatcc ctttgatcat aagggaatgt tgacaattag atcgattctc
      241 ctaggctgac tttcgcaata aatggggttg tcaataattg tgtatggtcg tttgccgtcc
      301 ggcggccgtc attcccgcgc aggcgggaat ccagaaacgc aacgcagcag gactttatcg
      361 gaaaaaaccg aaatcccacc gccgtcattc ccgcgcaggc gggaatccag caacacaacg
      421 cagcaggact ttatcggaaa aaaccgaaac cccaccgacc gtcattcccg caaaagcggg
      481 aatccagcaa ccgaaaaacc acaggaatct atcagcaaaa acagaaaccc caccgccgtc
      541 attcccgcga aagcgggaat ccagacccgt cggcacggaa acttaccgga taaaacagtt
      601 tccttagatt ccacgtccta gattcccgcc ttcgcgggaa tgacgagatt ttagattatg
      661 ggaatttatc aggaatgatt caatccatag aaaaaccaca ggaatctatc agcaaaaaca
      721 gaaaccccca ccgcgtcatt cccgcaaaag cgggaatcca gaaacacaac gcggcaggac
      781 tttatcggaa aaaacagaaa cccccaccac cgtcattccc gcgcaggcgg gaatccagac
      841 ccgtcggcac ggaaacttac cggataaaac agtttcctta gattccacgt cccagattcc
      901 cgccttcgcg ggaatgacga gattttagat tatgggaatt tatcaggaat gattgaatcc
      961 atagaaaaat cacaggaatc tatcagaaaa aacagaaacc cccaccgcgt cattcccgca
     1021 aaagcgggaa tccaaaaacg caacgcaaca ggaatctatc ggaaaaaaca gaaaccccac
     1081 cgccgtcatt cccgcaaaag cgggaatcca gacccgtcgg cacggaatct taccggataa
     1141 aacagtttcc ttagattcca cgtcctagat tcccgc
//
LOCUS       DAWFBO010000233         1148 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWFBO010000233 DAWFBO010000000
VERSION     DAWFBO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.233"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            220..891
                     /locus_tag="WGO87_001967"
     CDS             220..891
                     /locus_tag="WGO87_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0393275.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
     gene            953..>1148
                     /locus_tag="WGO87_001968"
     CDS             953..>1148
                     /locus_tag="WGO87_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0393276.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVL"
BASE COUNT          322 a          282 c          250 g          294 t
ORIGIN      
        1 acggatgaaa tcaaccgata ttcaaacaca gtcattttta gcgcattttc agcgtatcgt
       61 taatgcggaa aatttcgtga acaggttttt tgcacaggtc tccgcctcgt ttcgcgggat
      121 gggaaaccgt attagaaaac ggacgcacgc aacataaacc ccgaaagtga tgataagatg
      181 atgatttaac gtactgcttt aattatttaa ggaattatcg tgtttcccga caaatacaag
      241 ttgagtttag aagaaaatat ttttctggca aagaaagtat tggttgccca aattcacaac
      301 ctcagccgtt ttgagaattg tcagacgacc ttgttgcaga ccgaacaaat tatccatggc
      361 aaaaatgtag cctccgcgtc actggaagac atccaaacca tcttgaacct gaaacgtgcc
      421 tatcaatatg tgatttcgca tatttcaaac ggcgaaccgg tcgatatttc actccttaaa
      481 aaaatcaaca acattgttgc caaggacgat tctttggcac ccggtgattt ccgtaccggt
      541 tcggtcggcg taacgctatt ggacggttcc cgtcatgccc caaatccggt gaaggaaatt
      601 gaagtggctc gcgtgttaca aaatatcgga ctgcaaagcg gttcgacgac ggaggcagcc
      661 gtccgtttta tgctttattg tatgcggcag caggtttttt gggacggcaa caaacgaacg
      721 gcaaccttat ttgccaacgg tctgatgatg gcggggggct gcggcatctt ggaaatctcc
      781 gaaatgcaga tgccgcaatt caatgaaaaa ctgtccgcat tctatcgctc cggcgacgat
      841 accgatattt ccaagtttgt gtatcaaaat tgtatatcgg gcatagactg agacctttgc
      901 aaaattcccc aaaatcccct aaattcccac caagacattt aggggatttc tcatgagcac
      961 cttcttccgg caaaccgccc aagccatgat tgccaaacac atcgaccgct tcccactatt
     1021 gaagttggat caggtaattg attggcagcc gatcgagcag tacctgaacc gtcaaaaaaa
     1081 ccgttacctc cgagaccacc gcggccgtcc tgcctatccc ctgctgtcca tgttcaaagc
     1141 cgtcctgc
//
LOCUS       DAWFBO010000234         1122 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWFBO010000234 DAWFBO010000000
VERSION     DAWFBO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.234"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            80..1045
                     /locus_tag="WGO87_001969"
     CDS             80..1045
                     /locus_tag="WGO87_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0393277.1"
                     /translation="MLRLTALAVCTVLALGACSPQNSDSAPQAKEQAVSAAQSESASL
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT          287 a          317 c          295 g          223 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccta tgttacgttt gactgcttta gccgtatgca ccgtcctcgc
      121 cttgggcgcg tgttcgccgc aaaattccga ctctgcccca caagccaaag aacaggcagt
      181 ttccgccgca caatccgaaa gcgcgtccct gactgtcaaa acggcgcgcg gcgatgttca
      241 aataccgcaa aaccccgaac gtatcgccgt ttacgatttg ggtatgctcg acaccttgag
      301 caaactgggc gtgaaaaccg gtttgtccgt cgataaaaac cgcctgccgt atttagagga
      361 atatttcaaa acgacaaaac ctgccggcac gctgttcgag ccggattacg aaacgctcaa
      421 tgcctacaag ccgcagctca tcatcatcgg cagccgcgcc gccaaggcgt ttgacaaatt
      481 aaacgaaatc gcgccgacca tcgaaatgac cgccgatacc gccaacctca aagaaagtgc
      541 caaagagcgt atcgacgcgc tggcgcaaat cttcggcaaa caggcggaag ccgacaagct
      601 gaaggcggaa atcgatgctt cgtttgaagc ggcgaaaact gccgcacaag gcaaaggcaa
      661 aggtttggtg attttggtca acggcggcaa gatgtccgcc ttcggcccgt cttcacgact
      721 gggcggctgg ctgcacaaag acatcggttt gcccgctgtt gacgaagcca tcaaagaagg
      781 cagccacggt cagcctatca gctttgaata cctgaaagag aaaaatcccg actggctgtt
      841 tgtcctcgac cgaagcgcgg ccatcggcga agagggtcag gcggcgaaag acgtgttgga
      901 taatccgctg gttgccgaaa caaccgcttg gaaaaaagga caggtggttt accttgttcc
      961 tgaaacttat ctggcggccg gcggcgcgca agagctgctg aatgcaagca aacaggttgc
     1021 ggatgcgttc aacgccgcga aataatgaaa cggcggcatt cgatgccgtc tgaaacacgg
     1081 atgcaaaccg cttcccgtgt ttcagacggc attgcccgat ac
//
LOCUS       DAWFBO010000235         1112 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWFBO010000235 DAWFBO010000000
VERSION     DAWFBO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.235"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            153..1052
                     /locus_tag="WGO87_001970"
     CDS             153..1052
                     /locus_tag="WGO87_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0393278.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIETVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          189 a          253 c          354 g          316 t
ORIGIN      
        1 gggttttatc tggcaggacg gttttttgcc ccaacggaaa atagcctgcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt caggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg tcagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gacggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtctt aatattgatt ccatcaaggc agtattggca
     1081 gttttccgtg caaaacctgc gttaccggac tg
//
LOCUS       DAWFBO010000236         1035 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWFBO010000236 DAWFBO010000000
VERSION     DAWFBO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.236"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(299..685)
                     /locus_tag="WGO87_001971"
     CDS             complement(299..685)
                     /locus_tag="WGO87_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0393279.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(682..930)
                     /locus_tag="WGO87_001972"
     CDS             complement(682..930)
                     /locus_tag="WGO87_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0393280.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
BASE COUNT          284 a          243 c          182 g          326 t
ORIGIN      
        1 cagccatata tattaagtac ctgctcttta tgcgcttttt taggaaagga agatcaaacg
       61 gttcaatagc gaagtcttgc ccaaatttga tggacaggat atagaataca tgcgtgcata
      121 ccacaccctg ccaaagttgg gagatgcact taaggttgca cttgtggtcg ccaactatgc
      181 ctatacacgc ggatttcccg cgtttatagg ccgcgacgga agtctggctt ttaagcgaag
      241 acaataaccg aaaagaccgc ctcggcggtc tttcttgtgt acacagtttt tatatttgtc
      301 agcttgatgc attgacaact ctaattccat attgcggaat atattcatcg acagtcatca
      361 gttcaaagcc ttccgcttgg gtttgtgcaa tcaacatcct atcgaaaggg tctttgtgta
      421 tttccggaag gcttccagcc tgttttgcat gaaacagacc tataggcagc atttcaaaat
      481 cctcttcttg aagcacatca aaaaactctt ccggtaattt caacaacccc ttgttctgct
      541 tgatggaaat ttcccaaata cttgctgcac tgacaaagat cgcatttctc ggattttcta
      601 tcagtttgcg tgcagatatc cccagtttct tgtcatccaa caaccaccac agcaaagcat
      661 gggtatcaag cagaatcttt ctcacagagc cgactcctca aaaaataaag ctgccgtttc
      721 attgtcatcc tcaagaatac gtgaaatatc cgtattttcc atatgactga attttttcaa
      781 ccttcctgca tttcgtgccg gtttttcaat accggttagt tggacgcaag gcttacctgc
      841 cttcgcaata ataacaattt cccctgcttc tgctctttga atcaattgac tcaaattggt
      901 ttttgcctga tgaatatttg cttgaaacat aacacttctc atgattagct aacttaacta
      961 atatacatca ttaccaagat tttgggaatc tcattacata tatttgatta tatccgccgt
     1021 tttattcaca ccttg
//
LOCUS       DAWFBO010000237         1027 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWFBO010000237 DAWFBO010000000
VERSION     DAWFBO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.237"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..778)
                     /locus_tag="WGO87_001973"
     CDS             complement(<1..778)
                     /locus_tag="WGO87_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0393281.1"
                     /translation="MIYIDPPYNTGEDSFKYNDKFSHSTWLTFMKNRLEIAKDLLKDD
                     GLIFVQCDDKEQAYLKILLDEIFTRENFINCIAVKMSEPSGNKMAHTSHRLPKIKEYI
                     LIYKNINIKLNPIREQKSEWDDEYNIFLENFTQEDKRFIDLIISSQTENKELNADTLK
                     EIDVLLKKISPISVNQKLAQLNIKDNEVIKWKLDNAYRIIRTAASSSVKKLADEKKEI
                     CQQQFFSVISKRDKLLYIVKSDYSKDAKAPRVQVLFAEDYL"
BASE COUNT          296 a          164 c          150 g          417 t
ORIGIN      
        1 tcaaataatc ttctgcaaac aaaacctgca ctcgtggtgc ttttgcatct tttgagtaat
       61 ctgacttaac gatatacaat aatttatctc gtttagaaat aactgaaaaa aattgttgtt
      121 ggcaaatttc ctttttttca tctgctaatt tttttacgct actactagct gctgttctta
      181 taattctata tgcattatct aatttccatt ttataacttc attatcttta atatttaatt
      241 gggctaattt ttgattaact gaaataggag atattttctt taaaagaaca tcaatttctt
      301 ttaaagtatc agcattcagt tctttatttt ctgtttggct acttattatt aaatcaataa
      361 atcttttatc ttcttgtgta aaattctcta aaaaaatatt gtactcatcg tcccattctg
      421 atttttgctc tctaataggg tttaatttta tatttatatt tttatagata agaatatatt
      481 cctttatttt tggtagtcta tgcgatgtat gcgccatttt gttacctgat ggctctgaca
      541 tttttacagc aatacagttt ataaaatttt ctcttgtgaa aatttcatct aataaaattt
      601 ttaaataagc ctgttcctta tcatcgcatt gaacaaagat tagcccatca tcttttaata
      661 aatctttcgc aatttctaaa cgatttttca taaaagtaag ccaagtggaa tgactgaatt
      721 tgtcgttata tttaaaactg tcttcccctg tattgtatgg cgggtcaata taaatcagct
      781 tcactttacc ttttaactgt ttggcaagcg aatgcagggc aatcagatta ttgcctttga
      841 taataagatt ttcggcgggt gtgccgtctg aatgtcgttt gatttctcca acggcttgtt
      901 tgccgtctgc ggtatagcga gagaattttg agaatgcttt tgcgtcaaaa agccgatcga
      961 tttcatcaaa agcaagcgtt tgattaaaaa agatttcttg tctctttcgg gttaatttgg
     1021 tgtataa
//
LOCUS       DAWFBO010000238          982 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWFBO010000238 DAWFBO010000000
VERSION     DAWFBO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.238"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..516
                     /locus_tag="WGO87_001974"
     CDS             <1..516
                     /locus_tag="WGO87_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0393282.1"
                     /translation="RGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLD
                     EGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFA
                     NYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRT
                     VGAGVVSSVIA"
     gene            534..845
                     /gene="rpsJ"
                     /locus_tag="WGO87_001975"
     CDS             534..845
                     /gene="rpsJ"
                     /locus_tag="WGO87_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0393283.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
BASE COUNT          266 a          242 c          243 g          231 t
ORIGIN      
        1 cgcggtacag tagtaaccgg ccgtgtagag cgcggtatca tccacgttgg tgacgagatt
       61 gaaatcgtcg gtctgaaaga aacccaaaaa accacttgta ccggtgttga aatgttccgc
      121 aaactgctgg acgaaggtca ggcgggcgac aacgtaggcg tattgctgcg cggtaccaaa
      181 cgtgaagacg tggaacgcgg tcaggtattg gccaaaccgg gtactatcac tcctcacacc
      241 aagttcaaag cagaagtgta cgtattgagc aaagaagagg gtggccgcca taccccgttc
      301 ttcgccaact accgtccgca attctacttc cgtaccaccg acgtaaccgg cgcggttact
      361 ttggaagaag gtgtggaaat ggtaatgccg ggtgagaacg taaccattac cgtagaactg
      421 attgcgccta tcgctatgga agaaggcctg cgctttgcga ttcgcgaagg cggtcgtacc
      481 gtgggtgccg gcgtggtttc ttctgttatc gcttaattga aggatattga taaatggcaa
      541 accaaaaaat ccgtatccgc ctgaaagctt atgattacgc cctgattgac cgttctgcac
      601 aagaaatcgt tgaaactgca aaacgtaccg gtgccgttgt aaaaggtccg attccgttgc
      661 cgaccaaaat cgaacgtttc aacattctgc gttctccgca cgtgaacaaa acttcccgtg
      721 aacaattgga aatccgcacc cacttgcgcc tgatggacat cgtggattgg accgataaaa
      781 ctaccgatgc gctgatgaag ctggatttgc cggccggtgt tgatgtagaa atcaaagtcc
      841 aataattcgg actattgaaa atccccaagc aatcaatgct tggggatttt ttatgttatg
      901 ccgagacctt tgcaaaattc cccaaaatcc cctaaattcc caccaagaca tttaggggat
      961 ttctcatgag caccttcttc ca
//
LOCUS       DAWFBO010000239          978 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWFBO010000239 DAWFBO010000000
VERSION     DAWFBO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.239"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            9..>978
                     /locus_tag="WGO87_001976"
     CDS             9..>978
                     /locus_tag="WGO87_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ0393284.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSVGVVGTPDKLAGESETVLKKF
                     VYECPMLNEILDKAVWENDFPFRSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFRTYSGLN"
BASE COUNT          230 a          290 c          253 g          205 t
ORIGIN      
        1 aagtctgcgt gttggaaaaa cagcactttc cgcgcttcgt catcggcgaa agcctgctgc
       61 cgcactgtat ggaaatgctg gaagaagccg gatttgccga tgccgtgcgc gccgagcccg
      121 gctttcagtt gaaaaacggc gcggcgtttt cgtggggcag ccgttatacc gaattcgatt
      181 tcaccgataa attttcagac ggccccggca cgacttatca agtgcgccgc gccgtgttcg
      241 ataaaatcct gattgaagaa gccgccaaac aaggcgttga agtacgtttc gggcacggcg
      301 taaccgcgtt cgacaacagc ggcgattttg tccgcctgaa catcgaaacc gacaccggca
      361 agcgctatga actgaccgcg aaattcgtct tggacgcaag cggttacgga cgcgtgctgc
      421 cgcgcctgct aaacttggaa acgccctcgc acctgccgcc gcgccaagcg catttcacgc
      481 acatcgacga caacatcacc aacccgaaat tcgaccgcaa caaaatcctg attaccacct
      541 atccgcaaca ccgcgacgtg tggatttggc tgattccctt cggcgacaac cgctgttccg
      601 tcggcgtggt cggcacgccc gacaaacttg ccggcgaatc ggaaacagtg ttgaaaaaat
      661 ttgtttacga atgcccgatg ctgaacgaaa ttttggacaa agccgtttgg gaaaacgatt
      721 ttccgttccg ctccatccaa ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt
      781 tcgcgctatt gggcaatgcc gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca
      841 tcgcgctgca ctccgccaaa cttgccgccg atctgttggc aaaacaactc aaaggcgaag
      901 ccgccgattg gcaaaccgaa tttgccgaac ccttgatgat cggcgtggac gcgttccgca
      961 cctatagtgg attaaatt
//
LOCUS       DAWFBO010000240          961 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWFBO010000240 DAWFBO010000000
VERSION     DAWFBO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.240"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            180..509
                     /locus_tag="WGO87_001977"
     CDS             180..509
                     /locus_tag="WGO87_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0393285.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLEDGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            538..795
                     /locus_tag="WGO87_001978"
     CDS             538..795
                     /locus_tag="WGO87_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0393286.1"
                     /translation="MASSNVNKEIKDKKLSLWAKRQDGSVKWFCGQPVTRTTAGAKDD
                     TVAADTDTAKKIDTKHLPSTCRDAASAVCIETPPTAFYKNT"
BASE COUNT          285 a          263 c          228 g          185 t
ORIGIN      
        1 aaatttaatc cactatattt ccatgaaaaa gcatggaacc tttacgaaaa aaataaaaca
       61 gtcaaacaaa cacagccgtt tcagctgcac cggagcagct gacgtttgag cggcatttga
      121 cttcgccgga cggtatgtta cccttgccgt tttaaccaac aagcaacgaa aggacaaata
      181 tgggcagcct gattattgaa gatttgcagg aaggcttcgg aaaagaagca gttaaaggca
      241 aagagattac cgtgcattac acaggttggc tggaagacgg caccaaattc gactccagcc
      301 tcgaccgccg ccagccgctg accatcacgc tcggtgtcgg acaagtcatc aaaggctggg
      361 acgaaggctt cggcggaatg aaggaaggcg gcaaacgcaa gctgaccatc ccttcggaaa
      421 tgggctacgg cgcacacggc gcgggcggcg tgattccccc gcacgccact ttgatatttg
      481 aagtcgagct gctgaaagtg tacgaataaa gctgcccggg caataccgtc tgacataatg
      541 gcttcaagca acgtaaacaa agaaatcaaa gacaaaaaac tctccctgtg ggccaagcgt
      601 caagacggtt cggtaaaatg gttctgcgga cagccggtta cgcgcaccac cgccggcgcc
      661 aaagacgaca ccgtcgccgc cgacaccgac accgccaaga agatcgacac caagcacctg
      721 ccgtcaacct gccgcgacgc agcatctgcc gtttgcatag aaacaccacc tacggctttc
      781 tataaaaata cctaaaccgt cattcccacg aacctacatc ccgtcattcc cgcaaaagcg
      841 ggaatctagt tcgttgagtt tcagttattt agaataaatt ttgaaacttt aatcccgtca
      901 ttcccacgaa agtgggaatg acgggattta atgatgccgc cagcgtggca tcctccaccg
      961 a
//
LOCUS       DAWFBO010000241          950 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWFBO010000241 DAWFBO010000000
VERSION     DAWFBO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.241"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..406)
                     /locus_tag="WGO87_001979"
     CDS             complement(<1..406)
                     /locus_tag="WGO87_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0393287.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRMVINHHLALAADEVFEGSVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLS"
     gene            complement(526..732)
                     /locus_tag="WGO87_001980"
     CDS             complement(526..732)
                     /locus_tag="WGO87_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393288.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          249 a          251 c          180 g          270 t
ORIGIN      
        1 tgctcagact atcggtataa acaatactgt ccggcatgat tttctttttg atgacaggga
       61 gtaacgtttc agacttggca ttatccacca caacggtata gacccgtccg ttgcgtttca
      121 gaatgccgaa gacaaccact tttcctgccg caccgcgacc acgtctgcct ttacgccgtc
      181 cgccgaaatc gctttcgtcc ggctcgacag agccctcaaa aacctcatcg gcagccaagg
      241 ccaaatgatg gttgataacc atgcggattt tacggtagaa cagtgctgcc gaattgggat
      301 ggatacccaa aatatcggcg gcagaacggg cggtaacttc gagtacaaaa aaacggagca
      361 gttctttctg tacttttttc tttaatttgc agtgcgttat cttcatattt cgagggtaac
      421 atatctgcta atctagtaca gcccctagtt taaagctttg tttcttaagt ccgcagagta
      481 tgccatggtt agaccttcaa agttgagtat tgtactattt tgtttttaat taactataaa
      541 acgcttgtgt gaataggaag actgcagaat attgctgcct tttcctgttt gctttaaata
      601 aacagaaaat gccgtctgaa accgattttc agacggcatt tgtcccttaa aacacgtttt
      661 ttcaagcgcg accataccaa acacccacag gttgccttcc acccttcgta caacctttgc
      721 tccatcaaac atccacttcc taaatactga tgtctgctta ggttgaatcg gcattcgtac
      781 gattacggca caccgcctaa tctgtacctg cgtctgttgc gcccaatacc aaagactacc
      841 tgaaaatctc ccaaactttt caggtagcct tatcacgttt tcgactgtta ccacacgccc
      901 aaagaccgcc tgaaaccctt tttcagacgt tccgctccga taccccccaa
//
LOCUS       DAWFBO010000242          934 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWFBO010000242 DAWFBO010000000
VERSION     DAWFBO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.242"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(112..744)
                     /gene="mtrR"
                     /locus_tag="WGO87_001981"
     CDS             complement(112..744)
                     /gene="mtrR"
                     /locus_tag="WGO87_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0393289.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          210 a          205 c          243 g          276 t
ORIGIN      
        1 ctatgttatt gcaaaggtct caatatgtat ttttagaaga aggctttgtg tcattttgat
       61 gccgtctgaa agggtttgtt cgtttcagac ggcattgtta ccaaggcttg attatttccg
      121 gcgcaggtcg ggatggtttt ccaagttgtc catcattatc ccgatgatgc gcggggcggt
      181 tttgcccaaa tcgaaacgtt cgcaggaaga gaaccaccgc caaatcagcc cgtccaaggt
      241 tgatttgatg aagatgaccg ctgtttcctt gtccaaatcg tcagccaaat cctgattttc
      301 caccgcttcg gtcaaaacgg cggtaatttt ctcgcgccag attgcctgat gcttgcgggc
      361 aatggcgata acggcggcgt tttgttccgt gtgttcgcat tttaaaaaca ggatgttgtg
      421 gaatttgtag tagatgtcgt tgctttgcag ccgctcgaaa aagtgcagca gcgtgtggcg
      481 gaataccgcc caagaccctc cttcggcatc ttcggcatct tgcgcgatgc agttttcgat
      541 gtcgtcgcag atacgttgga acagcgcgtc gaacaagtct tccttatttt tgaaatgcca
      601 atagagcgcg ccgcgcgtta cgccggcggc ttgggcgatt tcgttgagcg aggtgcgcgc
      661 aatccctttg cggtaaaagg tttccaaggc ggcaagcatc aggtgttctt tggttttcaa
      721 ggcttcggtt ttggtttttc tcataatggc gttttcgttt cgggtcggtt tgatgagggc
      781 ggattataaa aaagactttg taaccatgca atcgtgtatg tataatgaaa cccatgaaat
      841 tgagactaca tctcaacttt gaaaacaggc ataacctgct ttgcaacccg ttcgaacatt
      901 cgggtatcaa agcaggcggt ttttatatcc tgaa
//
LOCUS       DAWFBO010000243          919 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWFBO010000243 DAWFBO010000000
VERSION     DAWFBO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.243"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..216
                     /locus_tag="WGO87_001982"
     CDS             <1..216
                     /locus_tag="WGO87_001982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393290.1"
                     /translation="ADAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQG
                     NKPLGAEELADLKPLYKDLKNNIGNIV"
     gene            547..>919
                     /locus_tag="WGO87_001983"
     CDS             547..>919
                     /locus_tag="WGO87_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393291.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGL"
BASE COUNT          288 a          147 c          215 g          269 t
ORIGIN      
        1 gctgatgccg gagacgtata ttgcgtctat aacatcagac tgaagcagta cactgcctgc
       61 caggttaccc gagttgaaga acacggtggc aaaaaaacac atgcgaccct gctggctttg
      121 gactggcagg gcaacaaacc gcttggggcg gaggagctgg cggatttgaa accactttac
      181 aaagacttaa agaataatat tggaaatatt gtatgaacaa aaaattaaac tatattttta
      241 tgttggactg tttagggttg gtgatattgt ttacttgtat aatagctact tttgaaagag
      301 attatggatt taaaattttt actaattcta agaggcctga attttattat tggattggaa
      361 tgttttatta tggaattatt tcttgctggt ttgattatca attaatttca acaaaggcga
      421 attcgtataa aagaaaagtt aaacaatata aaattttttc agtaatattt tcagttttga
      481 tatttatttc tactatagta aaactttaaa ttttggagca aaaatttatg agcgattcaa
      541 ttgaatatgt attgggaacg cggtctgcac atgtataagg caagtgctgt cgtgccaacg
      601 ggatatgtac gggttgggaa taccgcgccg ctggtcggcg aagacacgca acggtatgcc
      661 tctttttggg gcgacggcta cgacgtgtac cgtcagttga gatggcagca gatacccgaa
      721 aaacagagaa aggcattcaa aaaagccgcc aaaagcaaaa agaccgtgat gtttgccgga
      781 cgggaatacg gcatatccaa acagaatttg agcgatgttt gggatgattt tgaagacgcg
      841 atggaactga aggcgtttcc ctgcctgtct tcgctgtttc tgaccaaatg gcataaaaat
      901 ctatatgata gtggattaa
//
LOCUS       DAWFBO010000244          914 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWFBO010000244 DAWFBO010000000
VERSION     DAWFBO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.244"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            313..873
                     /locus_tag="WGO87_001984"
     CDS             313..873
                     /locus_tag="WGO87_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ0393292.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          257 a          259 c          183 g          215 t
ORIGIN      
        1 ctataccacg atgaatcctt cgcaatatct gtttatcgac ctcaattttg acaaaatacc
       61 ggatacgcgc ctttgttgct tttccatctt ccaaccaact gtaaatctca aacagccggt
      121 acacgccatg cttcagtttc ttttcctgtc ggcggattgt ttcgacaaag aattgaaaat
      181 ccatttcatg caccttaaaa tttaatctgc attcaaacct tttcactttg gaagcaccat
      241 ttatcggatg tcccttcgca ataaacaaat tttcccgata tcgccgccca tttcaaccca
      301 aacccaaaag ctatgaaaaa cctcatcgcc ttcaacaaac cctatggcgt tatctgccaa
      361 ttttcaccgc acgaaaaaca caaaagcctc aaagacttta tcaatcttcc cggcttctac
      421 cccgccggac ggctcgacac cgacagcgag gggctgctgc tgctgaccga cgacggcagg
      481 cttcaggcac aaattaccga ccccaaattc aaacacccca aaacctactg ggcgcaactg
      541 gagggcgtac ccgacgaaag ccgattggaa agcctaagaa aagggataga cttaggcggt
      601 ttcgttaccc gtccggcaag catccgcatc ttgaaacacg gagaagcaga ttcgttatgg
      661 gagcgcatcc cgccgatacg cgtccgcaaa accgttcccg atttttggat tgaaattacc
      721 atttctgagg gcaaaaaccg ccaagtcagg cgaatgaccg ccaaggcggg ctatccctgc
      781 ctgcgtctga tcagagtggc aagcggcagg ctgaaactgt ttgatttgga tttaaaaccc
      841 ggggaatggg catacgcccc gtttaaacca taatcacgtt tatctcatca tttccacaaa
      901 agtgggaatc cgga
//
LOCUS       DAWFBO010000245          911 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWFBO010000245 DAWFBO010000000
VERSION     DAWFBO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.245"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(66..845)
                     /locus_tag="WGO87_001985"
     CDS             complement(66..845)
                     /locus_tag="WGO87_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0393293.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          221 a          290 c          180 g          220 t
ORIGIN      
        1 gtactggttt aaatttagtt cactataccg atacatccga atatgccaga ccgtctaaac
       61 ggtattcata cctcccaatc ctgcacgcgc ttcaaatcgt tttgcgccaa agtatccgcg
      121 tggcggttac ggctctgata ttccctgtct ttcatgatgc tgcttgccac ataattcaaa
      181 tgtgcctttg ccgcctccga agcctcgccc ggccgacggt tcgatatcgc ctcatacagc
      241 acgcggtgct gcgccatcag tttcggacgc ggatcttctt cctgattcag ataaataagg
      301 ctgctgcgcg tctgccggta cagcattttc aacaaaccgc ccgacaaatg gctgaacaac
      361 aaattgtgcg ccgcatccgc aatcgtctga tggaagctga catcggcttc gctctgatgt
      421 tccagattgc cgctttcgca cgcctcctca aacttttcaa gccaaaaccc aatccgcttc
      481 aaatcggcat ccgtccggcg ttctgccgcc aatgccgcca tacagccctc gatgtggcag
      541 ctgaaatcaa aaacatcctg ttcccaattg gaatgcttgc ccaaaagctc ctgccaactt
      601 tgcaaaaaat cctgctgcgg cttgaccgaa acataataac cgtctccctg cctcgcttcc
      661 aaaacctgac gggcaaccaa aacattcaat gccgaccgga ccgacgggcg cgaaacgccg
      721 aactcttccg ccaaaacgcg ttcgggcgga atcttgcccc cttccgcgta aaccccttcc
      781 gcaatgcgct cctccaatac cgacaatacc tgatcgctga ttttctgagg ccttaccagt
      841 ttcattactc ctcctttata aagattccct gcagaaccct tccgaaatat agtggattaa
      901 caaaaatcag g
//
LOCUS       DAWFBO010000246          873 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWFBO010000246 DAWFBO010000000
VERSION     DAWFBO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.246"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            134..>873
                     /locus_tag="WGO87_001986"
     CDS             134..>873
                     /locus_tag="WGO87_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEZ0393294.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYM"
BASE COUNT          233 a          202 c          233 g          205 t
ORIGIN      
        1 cgtctgaagc cttcgcgcct cccagatgcc gtctgaagta cgctccgcgc cttcagatgg
       61 catttgattt agttgaacgg gtaggatgaa ttggttaaac ttgcgggcaa tagtgccttc
      121 agacaaacaa aatatgccga acaaaaagaa aaaagccgcc tttgcgcgtg ctgcaacgga
      181 gaacggtgtg ccggacgtgt ccaacatcaa aaattcatat gatgatttga tgtatgaatc
      241 caaagcattt tcccaaaccg ccatcaacgc cttggaggct cgcgcccgca tgatgggttt
      301 gcagcccgcg cccgccgcca atgccaaggt cttggagctg ggctgttcga tgggcggcaa
      361 catcatcacg caggctcttt attacccgga tgcggaattt gtcggtatcg acttgtccgg
      421 caggcaggtt gcgcagggca acgccatcat tgaaaaaatg ggcttgaaaa atgtgcgtct
      481 ggaagaaaaa gatattttga ccgtcgatga gtcattcggg aagtttgact atatcatcgt
      541 ccacggcatt tggtcgtggg tgcctgacgc agttaaagac aaaatttttt cgatttgccg
      601 gaacaacttg accgaacacg gcattgccta tatttcatac aatgtttacc ccggctggaa
      661 acggcaggag cagttgcgcg aaattatgta ctttgccggc agggatgtgc ttgaagaacc
      721 cttggaagca cggacgcgga aaggcttgga cgcgctcaag gcgctggcgg aaattttaga
      781 aaacgacaag ggcttggacg gcggcggcaa acttccggcg attcaaaaaa tattgaatca
      841 taatttttat tacatcgcac acgaatatat gga
//
LOCUS       DAWFBO010000247          870 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWFBO010000247 DAWFBO010000000
VERSION     DAWFBO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.247"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            348..647
                     /locus_tag="WGO87_001987"
     CDS             348..647
                     /locus_tag="WGO87_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0393295.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFLFAVLTVLKLKGYQAGLYTLLIALAVAAFGFGMPAGMAVSSLPPQPH"
     gene            587..823
                     /locus_tag="WGO87_001988"
     CDS             587..823
                     /locus_tag="WGO87_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0393296.1"
                     /translation="MRLRDAGGYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRPMPDIHVYGTPRSLTATVKHIVD"
BASE COUNT          246 a          235 c          188 g          201 t
ORIGIN      
        1 atccactata catcaaacat caaattggac tgaccaatca gggcggattc taatgacacg
       61 ccgttcccgc cgtcaacggc atttacctcg caccgccccc gaaacacagg aaaaaactac
      121 accaaactac aatttttgtt tatgcaaata tttgttttga caggatttaa acgaaagctc
      181 cgattcaaat ctgccgaacc gcccaaaaat atatagtgga ttaacaaaaa tcaggacaag
      241 gcgacgaagc cgcagacagt acagatagta cggtaaggcg aggcaacgcc gtactggttt
      301 aaatttaatc cactctattg acctgaatat taaagtttcg caaagtaatg caacgttgct
      361 ttaattggtt tgaccactat tgccgacgat tagaaaaata ttttcggaga tgttcaatta
      421 tggaaacttg ggttcaaaac tacacggcaa tcggcggcag cctgtatctg actgccgccg
      481 ccgcactctt acccatcgtc tttttatttg ccgtgctgac cgtcctgaag ctgaaaggct
      541 atcaggcggg gctttatacg ctgctgattg cgcttgccgt tgccgcattc ggcttcggga
      601 tgccggcggg tatggcggtt tcttccctgc cgccgcagcc gcactgaccc aacgccccta
      661 cgccacgctg gatttgaccg cgcattacaa aatcggcaaa tccacccgca tcggtttgga
      721 ctttgaaaac gtgttcaaca aacgctaccg ccctatgccc gacattcacg tttacggcac
      781 gccgcgcagc ctgaccgcaa ccgtcaaaca tatagtggat taacaaaaat caggacaagg
      841 cgacgaagcc gcagacagta caaatagtac
//
LOCUS       DAWFBO010000248          828 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWFBO010000248 DAWFBO010000000
VERSION     DAWFBO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.248"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>828
                     /locus_tag="WGO87_001989"
     CDS             <1..>828
                     /locus_tag="WGO87_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393297.1"
                     /translation="RLEKFDRVALQHFSQYVDLINENNGRLPNTSEIERSYYKAVTEN
                     GVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQAVNNPNGSEWDNRKQLISA
                     LDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIGGWGNGIINDLYKSVAKRE
                     WTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKETKKRNGKYHVYDPLALDLD
                     GDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVRDLNGNGIIDNGAELFGD"
BASE COUNT          262 a          143 c          194 g          229 t
ORIGIN      
        1 ccgtttagag aaatttgatc gggttgcatt gcaacatttc agccaatatg tagatctaat
       61 taatgaaaat aatggtagat tacctaacac tagtgaaatt gagagaagtt actataaagc
      121 cgttaccgaa aatggtgttt cttctagtgc agctattgat ttagttatta atcgctcact
      181 tccggatatg gcagatggtt attgggcatt aggtttgggg atagaagccg aacgtatcca
      241 caatgcgcaa gcagtaaata atccgaacgg tagcgaatgg gataatagaa agcagttaat
      301 atctgcttta gataaaggat ttgatggatc ttttaaagag aagcatttta cttttttaca
      361 atctgtgata atggatgtaa caaagttagg tgttgaatat acaatagatg gttggcaaaa
      421 aattggaggt tggggtaatg ggataatcaa tgatttatat aaaagtgttg caaaaagaga
      481 gtggactgga atatttgaga tcgttaataa taacatcaag caatttagag atctgttccc
      541 aaatccggaa ggctggatcg atgatggtca ccaatgtttc gctccttggg ttaaagaaac
      601 taaaaaacgc aatggcaaat atcatgtcta cgaccccctc gccctagatt tggacggaga
      661 cggtatagaa accgttgcca ccaaaggctt tgcaggcagc ttatttgatc acaccaacaa
      721 cggtatccgc accgccaccg gttgggtttc tgccgatgac ggtttactcg tccgcgattt
      781 gaacggcaac ggcatcatcg acaacggtgc ggaactcttc ggcgacaa
//
LOCUS       DAWFBO010000249          805 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWFBO010000249 DAWFBO010000000
VERSION     DAWFBO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.249"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..743)
                     /locus_tag="WGO87_001990"
     CDS             complement(join(<1..524,524..743))
                     /locus_tag="WGO87_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0393298.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAK"
BASE COUNT          193 a          175 c          160 g          277 t
ORIGIN      
        1 cttagcatga ataatagagc aagccaagta aagcatcgat ttaaaattac gagctaattt
       61 ctcatagcga gtagcaatac tacgaaactg ctttaatcgt gcaaatgcat tctcgactaa
      121 gtggcgtaat ttataaaggt agctatcaaa atcagggtta ggtttcttgg catttttacg
      181 ctttggtata atagctttca taccgtgttc tatcgcttta tctctgattt cttgcgagtc
      241 ataccctttg tcagcgataa aatactgggc ttcttgtatg acttctatca agtcgtttgc
      301 aacttgaccg tcgtgcacgt tacccccagt gactttaaaa tcgagcggat ttccatgcga
      361 gtccacacat aggtgtattt ttgtcgtgtt tccgccacgg ctttgtccaa ttgctctatc
      421 gaaaccacgc cgagctccac ttgcatgttg atgacaccgt acataacttc cgtcgatgaa
      481 tacccattct ttgtcaattt cttttcgtag atcaaaaaaa aattctgcca caagcctttt
      541 ttagaccatc ggttaaagcg gttataagcg gttttccatg accctagctc aataggtatg
      601 tctcgccatg gtgcacctgt tcttagcttc catagtatgg cttccatcac ggtacggtcg
      661 ttcttccatt gatgacagcc gtgcgctttc atggttgttt gtaattgttc ccatatgttg
      721 tcagttattg cggttctcgc cattgtttgt gtttgcctat atttcgttgg aatatagggt
      781 taaaataggg ctcttttggg cttat
//
LOCUS       DAWFBO010000250          802 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWFBO010000250 DAWFBO010000000
VERSION     DAWFBO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.250"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            121..>802
                     /locus_tag="WGO87_001991"
     CDS             121..>802
                     /locus_tag="WGO87_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0393299.1"
                     /translation="MQPPKNLLFSSLLFSSLLFSSAAQAASEDSSRSPYYVQADLAYA
                     AERITHDYPKATGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAADYASYRKWN
                     NNKYSVNTKEVLRNNNGNRKELKTENQENGTFHAASSLGLSAIYDFKLNDKFDKFKPY
                     IGARVAYGHVKHQVHSVKKETTTTFLAPTQGATVPGKIVEGPFSKPAYHESHSISSLG
                     LGVIAGVGF"
BASE COUNT          236 a          216 c          161 g          189 t
ORIGIN      
        1 ttgttcatcc gctatattgt gttgaaacat cgccacaaac ctgatatagt ccgctcctac
       61 aacatcattg aaaatctttc ttttttaatc agttaaaacc gaatacggag ccgaaaatga
      121 atccagcccc caaaaaacct tctcttctct tctcttctct tctcttctct tctcttctct
      181 tccgcagcgc aggcggcaag tgaagacagc agccgcagcc cgtattatgt acaggcggat
      241 ttagcttatg ccgccgaacg cattacccac gattatccga aagcaaccgg tgcaaacaac
      301 acaagcacag taagcgatta tttcagaaac atccgtgcgc attccatcca cccccgggtg
      361 tcggtcggct atgatttcgg cggctggagg atagcggcag attatgccag ttacagaaaa
      421 tggaacaaca ataaatattc cgtcaacaca aaagaggtgc taagaaacaa taatggcaac
      481 aggaaagaac tgaagacgga aaatcaggaa aacggtacat tccacgccgc ttcttctctc
      541 ggcttatccg ccatttacga tttcaaactc aacgataaat tcgataaatt caaaccctat
      601 atcggtgcgc gcgtcgccta cggacacgtt aaacatcagg ttcattcagt gaaaaaagaa
      661 accacgacta ctttccttgc accaacgcaa ggcgctacag tgccaggcaa gatcgtagaa
      721 ggtccgttca gcaaacctgc ctatcacgaa agccacagca tcagcagctt gggtcttggt
      781 gtcatcgccg gtgtcggttt cg
//
LOCUS       DAWFBO010000251          792 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWFBO010000251 DAWFBO010000000
VERSION     DAWFBO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.251"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            1..>792
                     /locus_tag="WGO87_001992"
     CDS             1..>792
                     /locus_tag="WGO87_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0393300.1"
                     /translation="MNRQRTRYLREHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSL
                     ITRIDFNLFCRFDELSIPDYSTLCRYRNWLAQDDTLSELLELINRQLTEKGLKVEKAS
                     AAVVDATIIQTAGSKQRQAIEVDNKGQVSGQTTPSKDSDARWIKKNGLYKLGYKQHTR
                     TDAEGYIEKLHITPANAHECKHLPPLLEGIAEGTTVYADKGYDSAENRQHLKEHQLLD
                     GIMRKACRNRPLTETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARA"
BASE COUNT          224 a          237 c          183 g          148 t
ORIGIN      
        1 ctgaatcgtc aaagaacccg ttaccttaga gagcaccgcg gccgtcccgc ctatcccctg
       61 ttgtccatgt tcaaagccgt cctgctcgga caatggcaca gtctctccga tcccgaactc
      121 gaacacagcc tcattacccg catcgatttc aacctgtttt gccgttttga cgaactgagc
      181 atccccgatt acagcacctt atgccgttac cgcaactggc tggcgcaaga cgacaccctg
      241 tccgaattgc tggaactgat taaccgccaa ctgaccgaaa aaggcttaaa agtagagaaa
      301 gcatccgccg ctgtcgttga cgccaccatt attcagaccg ccggcagcaa acagcgtcag
      361 gctatagaag tcgataacaa aggacaagtc agcggccaaa ccacaccgag taaggacagc
      421 gatgcccgtt ggatcaagaa aaacggcctc tacaaactcg gttacaaaca acatacccgt
      481 accgatgcag aaggctatat cgagaaactg cacattaccc ccgccaatgc ccatgagtgc
      541 aaacacctgc cgcctttgct ggaagggata gccgaaggta cgaccgtcta tgccgataaa
      601 ggctatgaca gtgcggaaaa ccggcaacat ctgaaagaac atcagttgtt ggacggcatt
      661 atgcgcaaag cctgccgcaa ccgtccgctg acggaaacgc aaaccaaacg caaccggtat
      721 ttgtcgaaga cccgttatgt ggtcgaacaa agcttcggta cgctgcaccg taaattccgc
      781 tatgcgcggg ca
//
LOCUS       DAWFBO010000252          755 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWFBO010000252 DAWFBO010000000
VERSION     DAWFBO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.252"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..468
                     /locus_tag="WGO87_001993"
     CDS             <1..468
                     /locus_tag="WGO87_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0393301.1"
                     /translation="DHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELRRLSHKRIA
                     GLAGIAPHPREGGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFYQRLLSEGK
                     PYKVAVTACMRKLLTISNARMRDYFAENDTAENDTAENDTAENDTAENGIRTA"
BASE COUNT          182 a          186 c          219 g          168 t
ORIGIN      
        1 gaccacaccc acacgcattt tgacggcaaa gcccaagtgg cggagcaaat caaaggcatc
       61 ggttcgataa cgacggctac gctgatggcg atgctgcccg aattgaggcg gctgtcgcac
      121 aaacggatag cgggtttggc cggcattgcc ccgcacccga gggagggcgg ggaaaccaaa
      181 tttaaaagcc gctgctttgg cggaaggtct gcggtgcgta aggcactgta tatggctacc
      241 gtggcagcga cacgttttga accgcttatt cgggatttct accaacgcct gctgtccgag
      301 ggtaagccgt ataaggttgc cgttacggca tgtatgcgca aactgctgac gatatcgaat
      361 gcccggatgc gtgattattt tgccgaaaac gataccgccg aaaacgatac cgccgaaaac
      421 gataccgccg aaaacgatac cgccgaaaac ggtatccgga cggcttgatt tgagttttgg
      481 tatttttgcc cgacggggtg aaaaatacag ttgctacggc tcgatgaatc gtcagaaata
      541 cccgaaccgt cattcccgcg caggtgggaa tccagaccgg tcggtgcgga aacttatcgg
      601 gaaaaaaggt ttctccgtcc ctgaatcctg gattcccact ttcgtgggaa tgacagaatg
      661 taggttcgtg ggaatgacgt ggtgcaggtt tccgtgcgga tgaattcatc attcccgcgc
      721 aggcgggaat ctagtctgtt cggtttcagt tattt
//
LOCUS       DAWFBO010000253          749 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWFBO010000253 DAWFBO010000000
VERSION     DAWFBO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.253"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>749
                     /locus_tag="WGO87_001994"
     CDS             <1..>749
                     /locus_tag="WGO87_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0393302.1"
                     /translation="DRFPLLKLDQVIDWQPIEQYLNRQKNRYLRDHRGRPAYPLLSMF
                     KAILPGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNWLAQDDTLSE
                     LLKLINRQLTEKGLKVEKASAAVVDATIIQTAGSKQHQAIEVDNKGQVSGQTTPSKDS
                     DARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLSPLLEGLPEGTTVYA
                     DKGYDSAENRQHLEEHQLLDGIMRKACRNRPL"
BASE COUNT          214 a          235 c          167 g          133 t
ORIGIN      
        1 gaccgtttcc cgctattgaa gttggaccag gtgattgatt ggcagccgat cgaacaatac
       61 ctgaaccgtc aaaaaaaccg ttaccttaga gaccaccgcg gccgtcccgc ctatcccctg
      121 ctgtccatgt tcaaagccat cctgcccgga caatggcaca gcctctccga tcccgaactc
      181 gaacacagcc tcatcacccg catcgacttc aacctgtttt gccgtttcga cgaactgagc
      241 atccctgatt acagcacctt atgccgctac cgcaactggc tggcgcaaga cgacaccctg
      301 tccgaattgc tcaaactgat caaccgacaa ctgaccgaaa aaggcctaaa agtagagaaa
      361 gcatccgccg ccgtcgttga cgccaccatt attcagaccg ccggcagcaa acagcaccag
      421 gctatagaag tcgataacaa aggacaagtc agcggccaaa ccacaccgag taaggacagc
      481 gatgcccgtt ggatcaagaa aaacggcctc tacaaactcg gttacaaaca acatacccgt
      541 accgatgcag aaggctatat cgagaaactg cacattactc ccgccaatgc ccatgagtgc
      601 aaacacctgt cgccgttgtt ggaagggtta cccgaaggta cgaccgtcta tgccgacaaa
      661 ggctacgaca gtgcggaaaa ccggcaacat ctggaagaac atcagttgtt ggacggcatt
      721 atgcgcaaag cctgccgcaa ccgcccgct
//
LOCUS       DAWFBO010000254          733 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWFBO010000254 DAWFBO010000000
VERSION     DAWFBO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.254"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(53..619)
                     /locus_tag="WGO87_001995"
     CDS             complement(53..619)
                     /locus_tag="WGO87_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0393303.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMLREGLSGGADDVREAKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMATTVMVTVGLTAGRALGVLF
                     GKRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          218 a          252 c          147 g          116 t
ORIGIN      
        1 aaggcgaggc aacgacgtac tggtttttgt taatccacta tattttccga catcattgaa
       61 tcaaacccaa atgcgacaag agcgtccatg taccgatggc aatcaacacc aaacctccgg
      121 caaactcggc gcgtttgccg aacaatacgc ccaaagccct tcccgccgtc agcccgaccg
      181 taaccatcac cgtcgtcgcc atgccgatga cggcggcggc aaaggcgatg tttacctcca
      241 taaacgccaa gcccaccccg actatcatgg aatcaatact ggttccaaaa gcagtcagaa
      301 ccgtcaacca ccagctttcc cgtttggctt cgcgcacatc gtccgccccg ccggacaagc
      361 cctcacgcaa catcttcaga cccagcccgc ccagcaggac gaaagccgcc caatggtccc
      421 attcgctgat aaacggcttg gcataaaaac cgcccaccca gcctgccagc ggcgtgagcg
      481 cttcaaccgt gccgaacacc aaagccgttg ccgcaatttt gcgcggaggc attctgaccg
      541 ccgcaccctt tgccaatgcg acggcaaacg catccatcga catccccagc gcaatcaaga
      601 gcaaagcata aaaacccata ccgcacccgt cctcaaaaag ggcggattat agcaaaagca
      661 aaaaaatgca aaaacgccgc acgaaaaccc acatcccgtc attcccgcaa aaacaaaaaa
      721 tcaaaaactg aaa
//
LOCUS       DAWFBO010000255          728 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWFBO010000255 DAWFBO010000000
VERSION     DAWFBO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.255"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            20..>728
                     /gene="rpe"
                     /locus_tag="WGO87_001996"
     CDS             20..>728
                     /gene="rpe"
                     /locus_tag="WGO87_001996"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0393304.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEI"
BASE COUNT          178 a          171 c          210 g          169 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcggcccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gtgtgccgat cgatgtgcat ctgatggtcg aacccgtgga
      241 tgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat attgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtaa accccgggtt cggcggtcag agcttcattc cgtatacttt
      481 ggaaaaaatc agacaggttc gtgccatgct tgaccgctat gaagagaaaa gcggcagacg
      541 tattgccatt gaagtggacg gcggcatcaa aaccgacaat atcgccgccg ccgcccaagc
      601 cggcgcggat acctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatag
//
LOCUS       DAWFBO010000256          724 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWFBO010000256 DAWFBO010000000
VERSION     DAWFBO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.256"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(127..>724)
                     /locus_tag="WGO87_001997"
     CDS             complement(127..>724)
                     /locus_tag="WGO87_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0393305.1"
                     /translation="LSIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          165 a          207 c          193 g          159 t
ORIGIN      
        1 aaacacagtc aaacaaattt atatgcccca tcccttccga ataatttgaa aacacagccg
       61 ccaaaaacaa aaatgccgtc tgaaaacctt tcagacggca tttccaactt gatttcaggc
      121 agaaagtcag aacgcgatat agctgttcgg attaaccggt ttgccgtttt gccgcacctc
      181 gaaatgaagc tgcgttctag aagcgtcggt attgcccatc aaagcgacct gctgcccgcg
      241 tttgacctgc tggccttcgc cgaccagcaa tttttggttg tgcccgtatg cggtcaggaa
      301 ggaagaatta tgctggatga tgaccaaatt gccgtatccc ctcaaaccgg aacctgcata
      361 aaccactttg ccgtcagccg ccgccaaaac gggctgtccc gcatttcctg caatatcgac
      421 acccttattg ttgccgccga aatcggcaac cactttacct tgcgtcggac gctgccaaac
      481 aatgccgccg accgaacgcg tgccggaagg cgaagcggca ggagattgcg gggcgggcgc
      541 gggaaccgct ttattttccg cagcgggcgc ggcaggttgc ggcgcggact gcacaggcgg
      601 ttgcgcggcg ggtttcacag gggtttgcac ggcagccggt acggcgggcc tgctttctac
      661 ggctgaggtt ttcggtgcgg catatcctgc cggtttgact ttaacaatct gaccgatgct
      721 caac
//
LOCUS       DAWFBO010000257          709 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWFBO010000257 DAWFBO010000000
VERSION     DAWFBO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.257"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(97..492)
                     /locus_tag="WGO87_001998"
     CDS             complement(97..492)
                     /locus_tag="WGO87_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393306.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSYLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFAKVSG"
     gene            complement(476..>709)
                     /locus_tag="WGO87_001999"
     CDS             complement(476..>709)
                     /locus_tag="WGO87_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0393307.1"
                     /translation="VIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSDNS
                     EMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          195 a          155 c          130 g          229 t
ORIGIN      
        1 taacacctcc taattttacc caaatacccc cttaatcctc cccggatacc cgataatcag
       61 gcatccgggt taccttttag gcggcaacag gcgcacttag cctgagacct ttgcaaattt
      121 gtcggtttcg gtgtcgtatt ggtagcctcg tgcctgtatg tcttctttga aagtttcgta
      181 tacgtcatgg gctaaaaggg ctgttccgac atagggaacc gcccttgtgc tgaatttcgc
      241 gcctaagcgg gcaagtttgc cgccccccgc caatacgccg gcgcgggaaa tgctggcggt
      301 tattttggcg ttgattcggg cttttgcgcc cgtagggatg tgtgttaaat ctaccgtttt
      361 tattaaataa gatgaataag ttttactatt tttaggtaca aacttatgaa ttttcgcacc
      421 ttgtccggta tcaactgaaa cagtttcaga tatttttact gcatttgcat tcgcttcaaa
      481 cgaatacatc atcaaaattg caattatcga caatttcgca aaattcaaat ttgtatattt
      541 tatgaccatc tttcagggat tctttaatta ccatttctga attatcagaa aatgagatta
      601 gccaaatatc atgtttaatt cttctattcc agaaaaaaga gaaacaatca ataacatttt
      661 cagacttatt aatcttcgca aattcaacaa attcagattg cgctataac
//
LOCUS       DAWFBO010000258          706 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWFBO010000258 DAWFBO010000000
VERSION     DAWFBO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.258"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            28..705
                     /locus_tag="WGO87_002000"
     CDS             28..705
                     /locus_tag="WGO87_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HEZ0393308.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
BASE COUNT          218 a          137 c          156 g          195 t
ORIGIN      
        1 catcaaaaaa cctgataagc tcctaatatg aaactaaaaa ataagtatca aaagttcagc
       61 aaaatttcag agcaaaagtt tagagaaata atccgctgtt tcgctcttga tttgaccgct
      121 tccgatactg ctaaaatgac cggcattagt gtacgcggta tcaatcctat tttcctgaaa
      181 atcagacaca ggattgctgc tctgtgcgaa caaagctcac cactgtccgg tgtggtcgaa
      241 ttagatgaat cttactttgg tgcgcgacgt atcaggggta agcgcggtcg cggagcgtca
      301 ggtaaaacca tcgtatttgg catactgaaa cgcaatggcg tggtctatac ggaaatcgtt
      361 cccgacgctt cgaaagcctc actaatcaag gtcatacgtg gtcacatatc tgctgacagt
      421 gaaatcaata ctgacggctg gaaagcatat gacggtcttg tcgatatggg ctatgagaag
      481 cattaccgtg tccatcatgg ttccaatgag tttgctcgag gaaagcaaca tatcaatggt
      541 attgaatctt tttggagtta tactaagggg cgtttggcta aatttcatgg tatttccaaa
      601 gagatgtttt atctgcatct caaagaaacg gagtttaggt ttaaccaccg gcatgaagat
      661 ttaggtaaaa tattaatgaa gatgcttcga aataacccaa tttaat
//
LOCUS       DAWFBO010000259          662 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWFBO010000259 DAWFBO010000000
VERSION     DAWFBO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.259"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..249
                     /locus_tag="WGO87_002001"
     CDS             <1..249
                     /locus_tag="WGO87_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393309.1"
                     /translation="YPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKI
                     NERYERFRVMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
     gene            280..657
                     /locus_tag="WGO87_002002"
     CDS             280..657
                     /locus_tag="WGO87_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0393310.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGISTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
BASE COUNT          220 a           83 c          138 g          221 t
ORIGIN      
        1 tatccttatc cgatggatat accaagggac atcgtgattg gaatcggtac cattattgat
       61 tttttaatgg ttcaaaattg gaaacttttt gaaattaaag cttctccttg gttgcctaaa
      121 agtgtcaaaa ttaatgagcg ttatgaaagg ttcagagtaa tgctccgtta tatttttact
      181 gatctagatg tagaggactg gaaatttttt tactttccaa tacaacatag taaaggtaga
      241 ttgagatgaa aagtttaatt atattggaga tacattatta tgtgtgagtt caaggatatt
      301 ataagaaacg ttccttattt tgaggggtat gacgaaaatt catttattgg caaatggtat
      361 gatgacgggg tgtgggatga tgaagaatat tggaaattgg agaatgattt aatcgaggtt
      421 agaaaaaaat atccttatcc gatggatata ccaagggata ttgtgattgg aatcagtacc
      481 attattgatt ttttaatggt tccaaattgg gagcttttcg aaattaaagc ttccccttgg
      541 ctacctgata gcgtgggaat tcatgaacgt tatgaaagat tcacaacaat gctccgttat
      601 atttttaccg agaaagacat agtcaacgtg cgatttgatt attacaacaa aaaatagcaa
      661 ac
//
LOCUS       DAWFBO010000260          656 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWFBO010000260 DAWFBO010000000
VERSION     DAWFBO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.260"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(53..538)
                     /gene="fxsA"
                     /locus_tag="WGO87_002003"
     CDS             complement(53..538)
                     /gene="fxsA"
                     /locus_tag="WGO87_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0393311.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          196 a          212 c          116 g          132 t
ORIGIN      
        1 tttcaaaccg gaaaaatgcc gtctgaatct tcagacggca ttccattcat atttattcgt
       61 cttttttgtg ttcgatggcg tttcgggaac gattgccgcc gtaaggctct tcaaccgtat
      121 attccccctc gataatatcg tcatcgcggg aaaagccctc ttttctgccc gattggttca
      181 tgttgaaaaa attttccgca cctcctgcct gcaacactgc acctccctta aacggcagca
      241 gcagcaatac cgccaacacc gaggatacga atcccggact catcagacac accgccgcca
      301 ccgtataacg gataggccac aacatctgat aaacggatac cctcccgccg cttctcattg
      361 ccgcgcccgc caataaaaga ccggacagcc ccgtatgcct gagcatcagc acaccggcgg
      421 caaaacctgc cgccatcaaa aacaacgtcc agccgccgcc caaccaatcg gcaacccaca
      481 caatcgacat aatctccaaa aacagcagca ccaaaaaacc gataccgaaa aatctcattg
      541 accgtcatcc ttatatttaa gtaaacagca aaccgcccga acaagactcc aagcgacctg
      601 cctgtaaatg attacaaaac catgtgcttc aagccgaaac aatgtgaaat cacgca
//
LOCUS       DAWFBO010000261          636 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWFBO010000261 DAWFBO010000000
VERSION     DAWFBO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.261"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..584)
                     /locus_tag="WGO87_002004"
     CDS             complement(<1..584)
                     /locus_tag="WGO87_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0393312.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYHIYDPLALDLDGDGIETVATKGFSGALFDHRNQG
                     IRTATGWVSADDGLLVRDLNGNGIIDNGAELFGD"
BASE COUNT          160 a          160 c          136 g          180 t
ORIGIN      
        1 ttgtcgccga agagttccgc accgttgtcg atgatgccgt tgccgttcaa atcgcgcaca
       61 agcagaccgt catcggcaga aacccaaccg gtggcggtgc ggatgccttg gttgcggtgg
      121 tcgaacaatg cacctgaaaa gcctttggtg gcgactgttt ctatgccgtc gccgtctagg
      181 tctagggcga gggggtcgta gatgtggtat tggcctgaac ggtttctgtc caattctttc
      241 cacagattcc agccgtcggg cagccagtcg tatacccaac gagaccagtc ttcaaatagc
      301 tcatacccaa aacctttcca gttgtcaaaa ttataaaatt cctctgtaga tatcgtacca
      361 gtatctcctg tggcaatagc cgttgctgca attatatctc cagcaattgc taatggaatc
      421 ccccaactct gcatatttcc attcttctct attaatgtac ttcctaaccc tcccaatgaa
      481 tataaatccg ctaaaatatt ttgaaaacgg ttgatattaa ctttaccatc tttatgctcc
      541 tgaatagctg aagatatatt tccagagaga cggcttgcaa tcacagaggc atttgttccg
      601 tttacaatat gcgatgacgt actacctggt gcaata
//
LOCUS       DAWFBO010000262          627 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWFBO010000262 DAWFBO010000000
VERSION     DAWFBO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.262"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>627
                     /locus_tag="WGO87_002005"
     CDS             <1..>627
                     /locus_tag="WGO87_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0393313.1"
                     /translation="RSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQID
                     NAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA
                     DGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDD
                     CPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPI"
BASE COUNT          167 a          185 c          150 g          125 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggtggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggtgccgc acaaatggac ggtgcaatct tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gccaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 gaaaatcttc gaattggctg ctgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacct ttcttgttgc ctatcga
//
LOCUS       DAWFBO010000263          616 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWFBO010000263 DAWFBO010000000
VERSION     DAWFBO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.263"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>616
                     /locus_tag="WGO87_002006"
     CDS             <1..>616
                     /locus_tag="WGO87_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HEZ0393314.1"
                     /translation="IYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVG
                     IENRPAIVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAEDTARA
                     AVRALKAHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLI
                     RQYFPKQTDFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNL"
BASE COUNT          198 a          168 c          136 g          114 t
ORIGIN      
        1 catttaccgc taccttcgcc aagacaaaag caacggcagc acgttgtggc aacatctcag
       61 aatatgcagc aaaccctacc gcaaacgcta cggcagcaca tggaccagag gcaaagtccc
      121 caaccgtgtc ggcatagaaa accgacccgc tatcgtcgac cagaaatccc gtatcggcga
      181 ttgggaagcc gacaccattg tcggcaaagg acagaaaagc gcattattga ccttggtcga
      241 acgcgttacc cgctacacca tcatctgcaa attggatagc ctcaaagccg aagacactgc
      301 ccgggcagct gttagggcat taaaggcaca taaagacagg gtgcacacca ttaccatgga
      361 taacggcaaa gagttctacc aacacaccaa aataaccaaa gcattgaaag cggagactta
      421 tttttgtcgc ccttaccatt cttgggagaa agggctgaat gagaacacca acggactcat
      481 ccggcaatac ttccccaaac aaaccgattt ccgtaacatc agtgatcggg agatacgcag
      541 ggttcaagat gagttgaacc accgaccaag aaaaacactt ggctacgaaa cgccaagtgt
      601 tttattcttg aatctg
//
LOCUS       DAWFBO010000264          607 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWFBO010000264 DAWFBO010000000
VERSION     DAWFBO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.264"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            61..444
                     /locus_tag="WGO87_002007"
     CDS             61..444
                     /locus_tag="WGO87_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0393315.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
BASE COUNT          148 a          145 c          155 g          159 t
ORIGIN      
        1 ggcaacgccg tacaggtttt tgttaatcca ctatattgag aacattttga aaggataccg
       61 atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag gcggcatcag gcgcgtcatt
      121 aacgcattcg gctattcgat agacggcatc gccgccgcct accgttacga agcggcattc
      181 cgtcaggttt tgtggctgaa cgcgctgctg gtgtgtgcgg catttttttg ggtttccgaa
      241 accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt cggtcattgt cgaactgttc
      301 aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg aaaaacacga gctggccaaa
      361 cgcgccaaag acgcaggttc tgccgcacaa ttgttcgcga tgctgatgtt ggcggcggtt
      421 tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga tttgtaatcc tttaggatta
      481 gtatgtggta tttattattc acttaatcta catcaaattt ccaggcagta tttgtatgta
      541 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg
      601 aaccgat
//
LOCUS       DAWFBO010000265          600 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWFBO010000265 DAWFBO010000000
VERSION     DAWFBO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.265"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..151
                     /locus_tag="WGO87_002008"
     CDS             <1..151
                     /locus_tag="WGO87_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393316.1"
                     /translation="SIRQGKPEEEFSRKMAGCLTDLENCLEKYLEQFGPVSESIEACT
                     VKLQE"
     gene            173..466
                     /locus_tag="WGO87_002009"
     CDS             173..466
                     /locus_tag="WGO87_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393317.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          159 a          151 c          160 g          130 t
ORIGIN      
        1 gtccattcgg cagggaaagc ctgaagaaga attttcccgc aagatggccg gctgcctaac
       61 cgacttggaa aactgtttgg aaaaatacct cgaacagttc gggccggtat cggaaagtat
      121 cgaagcctgt accgtcaaac tgcaggagta accgtcgttc ttcaacaggc tgatgaaaac
      181 caacgacaag ctcaataggc agattgacgt gttgcagaaa cagtcggcag ccatccacaa
      241 cgaagcctat atcgaaatga acacgctgct ttaccgccac cgcgaagtgg tttccgtcca
      301 caaccgcaag gcggattatg cggaaaaggg caaagagcgg attgcgctgt tcccgcgcgg
      361 cttgaacggc atcaccaagc tgcctgccgc cgtcctgttg cccgagcgtc ccgattgttt
      421 tgatatgaag gcagtttggc aaatgtttaa tagaataccg aggtaggcgt atcggctgcc
      481 ggataaaaat gccgtctgaa acggaagcgt tcagacggca ttgttgcagt ggaacaaatc
      541 aaaatgcacc ccgtcattcc tgtctgtacg gtgatagcgg cagtgttttc ctgcttcgtt
//
LOCUS       DAWFBO010000266          578 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWFBO010000266 DAWFBO010000000
VERSION     DAWFBO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.266"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            169..>578
                     /locus_tag="WGO87_002010"
     CDS             169..>578
                     /locus_tag="WGO87_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393318.1"
                     /translation="MKTDIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLSNDELKRHFFVKVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRN"
BASE COUNT          192 a          116 c          119 g          151 t
ORIGIN      
        1 cgcaaaattc cgacacactc ggacacccgc cccgcttatc gcaactttgc gaacgccccc
       61 ggaaacagca aagacatcaa aggattgatt ttattagaat ttatttgcaa agccatttgc
      121 cgttacacaa gaatggcaca ttaaaataac tgatgaggat ttataacgat gaagacagac
      181 attcaaaccg aattaaccca agccctgcta tcacacgaaa aagtatgggc gaacgaagaa
      241 aaaaccattt tagccaaaaa cattctgttg gatttggtgg aaaaaaccga cccgaccatt
      301 atcggtttgt tattgagtaa tgatgagtta aaacgccatt tctttgtgaa agtgaatggt
      361 gtgctggtgt ttaaattgca ggatttccgt tttttcttgg acaaacacag cgtcaataat
      421 tcctacacaa aatacgccaa ccgcattggt ttgacggacg gcaaccgctt tttgaaagac
      481 agttcggata ttgtgttgga ttttccgttt aaagattgcg tattgaatgg cggacaaagc
      541 accgaagaag gcgaagagat ttattttaaa cgcaataa
//
LOCUS       DAWFBO010000267          555 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWFBO010000267 DAWFBO010000000
VERSION     DAWFBO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.267"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          184 a           98 c           77 g          196 t
ORIGIN      
        1 tagaaattag ttaaagaata tattatggta gatactaact ctttagttaa aaataattta
       61 ctaactttta ctacttaggt ataaattcca atacttaact aaactgaggg taaatatatt
      121 tatccaagat agaagagaaa cttttttctc tgtttccgca agaatagtta cgggttgtga
      181 aatatgctct atacccaagc agtagatatt ttcattgtta cttaccgtat tgcttcatag
      241 aattggcact ctacgctttc ctttaaaaat cgtcaataac gattatcatt ttctttaaat
      301 ttattcaaaa caatattctt atatacatct caagcccgct gaaataaagc aaaatggtgg
      361 ttcatcgact gtaatgccaa tttcaaagca gcctgcactt tgacaacaca caagatatac
      421 gggaaggaca gggcatcagc atatatttca gcccgaagcc gtttcattcg tttaaaaaac
      481 tgcttttttc acaatttacc tttacaaagc ctatacgtat ccttacaatt tgttttgctg
      541 tttttggtat atttt
//
LOCUS       DAWFBO010000268          549 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWFBO010000268 DAWFBO010000000
VERSION     DAWFBO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.268"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(16..507)
                     /locus_tag="WGO87_002011"
     CDS             complement(16..507)
                     /locus_tag="WGO87_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0393319.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT          161 a          138 c          115 g          135 t
ORIGIN      
        1 gaatccgaca ttacttcaat cgtatccaat agaaaagtcc gcattgccgc caccccaatt
       61 atgcggataa ataccctgtt tgacataacg gtgaaacgta gaaaaccccc aatcggaaat
      121 ttgtcctaca tagccatgtt tgaccggatt gaaatgcaga taatcaaaat gccaggcaaa
      181 atcggcctca tcgcggatag tatattccca aaagcgtttt tgccaaagcc tgagattgcc
      241 gccgattaaa tattggctgt gccgcttgat ttgccgccag cgttccgaat aagcagaatc
      301 attgtccggc agccgccata tggtatgaag atggtcgggc atcaacaccc atgccaaaat
      361 ttcaaacgga taccgttcgc gcaccgccat taccgcctgc cgcaaagcca aacgcaccgc
      421 atcatcggtc aaaatcttct gccgtttatt ggttacaacc gtaaaaaagt aagtgccgcc
      481 attgcggtaa aaacgacggt atttcatagt attatgctcg gaatgatttt gtaggtcgga
      541 ttcttgaat
//
LOCUS       DAWFBO010000269          507 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWFBO010000269 DAWFBO010000000
VERSION     DAWFBO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.269"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(323..398)
                     /locus_tag="WGO87_002012"
     tRNA            complement(323..398)
                     /locus_tag="WGO87_002012"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Glu,seq:ttc)
BASE COUNT          139 a          122 c          133 g          113 t
ORIGIN      
        1 aatactttga tgctttggtg ggctgaagcc caccctacaa tatagagaaa atgccgccaa
       61 acggtatgga agcggaaaca atggtggggc tggcttcaga aacaatggta gggtgggctt
      121 cagcccaccg ctttagacgg catccgcgca ccgtcattcc cgcgcaggcg ggaatccagc
      181 cttgtcggca cggaaactta tcggataaaa cggtttcttc aaccctacgc tctagattcc
      241 cgttttcacg ggaatgacga agagtgtgag aatgacggtt cataaataaa aaaacctgct
      301 taaaacaagc aggtttttga attggcacgc ccacggggat tcgaaccccg gttgccgccg
      361 tgaaagggca gtgtcctagg cctctagacg atgggcgcgt gggaatgggc attatacata
      421 cttctagaaa tttagcaata cgatgccgtc tgaaacttcg tcattcccgc gaaagcggga
      481 atctagaatc tccaagtttt cagacaa
//
LOCUS       DAWFBO010000270          494 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWFBO010000270 DAWFBO010000000
VERSION     DAWFBO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.270"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..494
                     /locus_tag="WGO87_002013"
     CDS             <1..494
                     /locus_tag="WGO87_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0393320.1"
                     /translation="KGNNITLFIADDVEIMGLVCSLHSDCSLQIQAKTTMGNGEITIA
                     EKGKISIGKDCMLAHGYEIRNTDMHPIYSLENGERINHGKDVIIGNHVWLGRNVTILK
                     GVCIPNNVVVGSHTVLYKSFKEPNCVIAGSPAKIVKENIVWGRKMYHSTMYDDPTLNE
                     FYK"
BASE COUNT          182 a           66 c           93 g          153 t
ORIGIN      
        1 taaaaggaaa taatataacc ttatttatag ctgatgatgt agaaatcatg ggactagtat
       61 gttcccttca ttcggactgc tctttacaaa ttcaagccaa gaccactatg ggcaatggag
      121 aaattaccat tgctgaaaaa ggtaagatta gtattggcaa agattgtatg ctggcacatg
      181 gatatgaaat tagaaatact gatatgcatc caatttattc tttagaaaat ggcgaacgaa
      241 taaaccatgg taaagacgtt attatcggta atcacgtgtg gcttggccgc aatgttacta
      301 tattaaaagg tgtatgtatt ccaaataatg tagttgtagg ttctcatact gtgctatata
      361 aaagttttaa agaacctaat tgtgtaatag caggttcacc tgcaaaaata gtaaaagaaa
      421 atattgtatg gggaagaaaa atgtatcatt caactatgta tgatgaccct actttaaatg
      481 aattttataa ataa
//
LOCUS       DAWFBO010000271          488 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWFBO010000271 DAWFBO010000000
VERSION     DAWFBO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.271"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(16..391)
                     /locus_tag="WGO87_002014"
                     /pseudo
     CDS             complement(16..391)
                     /locus_tag="WGO87_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          141 a          129 c          103 g          115 t
ORIGIN      
        1 gaatccgaca ttactttaat cgtattcaat agaaaagtcc gcattgccgc caccccaatt
       61 atgcggataa ataccctgtt tgacataacg gtgaaacgta gaaaaccgcc aatcggaaat
      121 ttgtcctaca tagccatgtt tgaccggatt gaaatgcaga taatcaaaat ggcaggcaaa
      181 atcggcctca tcgcggatag tatgttccca aaagcgtttt tgccaaagcc tgagattgcc
      241 gccgattaaa tatttgctgt gccgcttgat ctgccgccag cgttccgaat aagcagaatc
      301 attgtccggc agccgccata tggtatgcag atggtcgggc attcaacacc catgccaaaa
      361 tttcaaacgg ataccgttcg cgcaccgcca ttaccgcctg ccgtaaagcc aaacgcaccg
      421 catcatcggt caaaatcttc tgccgtttat tggctacaac cgtaaaaaag taagtgccgc
      481 cattgcgg
//
LOCUS       DAWFBO010000272          460 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWFBO010000272 DAWFBO010000000
VERSION     DAWFBO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.272"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            1..434
                     /locus_tag="WGO87_002015"
                     /pseudo
     CDS             1..434
                     /locus_tag="WGO87_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
BASE COUNT          172 a           87 c          104 g           97 t
ORIGIN      
        1 aaaaaaggcg acaccctgct ttccgaaagc ggtgcaaaac agacggttca aaacattacc
       61 ctcaaacagc agccgctcaa agcctacaat ctgaccgttg ccgactggca tacctacttc
      121 gtcaagggta atcaggcgga acggaagggg tttgggttca taatgcgtgt ccgccaagaa
      181 aaacaccatc tacccccgta tatgggaatg attcagaagc atatgcagca gcaaagaaat
      241 tgggatatag aaaaataaaa gaaagaacga gaaatgatgc tgctattttt aaaaaaggaa
      301 agagttatat atcacgcgat gtagattcac ataatggagg ggcttggaaa gaagcatctt
      361 cacccaagaa gctgaacaga aaagaaacaa gaaacggtac atttgataaa aacctaaatc
      421 gtattggaga ttaattatga tgtgcgagtt caaggatttt
//
LOCUS       DAWFBO010000273          457 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWFBO010000273 DAWFBO010000000
VERSION     DAWFBO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.273"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(379..454)
                     /locus_tag="WGO87_002016"
     tRNA            complement(379..454)
                     /locus_tag="WGO87_002016"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(419..421),aa:Ala,seq:tgc)
BASE COUNT          111 a           95 c           85 g          166 t
ORIGIN      
        1 gcctaacatt ccgttgacta gaacatcaga cttgaatttc ctactttgat aaagcttact
       61 gctttgttgt gtcttaatcc tgccttttgt gtttcaggat taagtcgata caatcatcac
      121 ccaaatacgg tgtttgtttt cttttctctt gtgagcgatt ttttatcctt tgcaaagaat
      181 aaaaaatcaa aacaaacgct ttgtctttgt ttgttgattt cggctttcca atttgttaaa
      241 gatcgatgcg ttcgatattg ctatctactg tgcaaatcaa aacgagctga ttattatatc
      301 agcattttgt tcttggtcaa gtgtgacgtc tccctgaatg gattctgttc cattcttccg
      361 ttttgatttg tacagtattg gtggaggcaa acgggatcga accgatgacc ccctgcttgc
      421 aaagcaggtg ctctaccaac tgagctatgc ccccatt
//
LOCUS       DAWFBO010000274          448 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWFBO010000274 DAWFBO010000000
VERSION     DAWFBO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.274"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(38..250)
                     /locus_tag="WGO87_002017"
     CDS             complement(38..250)
                     /locus_tag="WGO87_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0393321.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
BASE COUNT           95 a          125 c          118 g          110 t
ORIGIN      
        1 gcacttgaaa tccgaatcca agggcatttt tgccgcttta tttcaacgcg gcatcatagc
       61 cgccgtcttc aacggcttca atcaacgccc ctgcatcggt ttgcgcgggg tcgtatccga
      121 cggtcgcgct tttgttttca aggctgactt cgacgcttgc cacgcctttt acgccttcca
      181 atatccgggt aacgcttttg acgcagccgc cgcagctcat gccgccgatg tcgaggataa
      241 gggtttccat cattttcctt tcgttggaac tgcaatctga cgggcgttat tgtaagtcgg
      301 ggcgtgaact tgggcaaacg cggaaacggt gcggcggttt ggaaaaagac ggacgcttgc
      361 gcataatggc ggcaattccc atcaggacga caacaatgaa cgcttcacaa aaaccctggt
      421 tgagcatcat cgccttggca atcggcgc
//
LOCUS       DAWFBO010000275          430 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWFBO010000275 DAWFBO010000000
VERSION     DAWFBO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.275"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>430
                     /locus_tag="WGO87_002018"
     CDS             <1..>430
                     /locus_tag="WGO87_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0393322.1"
                     /translation="ADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDNHT
                     HTHFDGKDQVAEQIKGIGSITTATLMAMLPELGRLLHKRIAGLAGIAPHPREGGETKF
                     KSRCFGGRSAVRKALYMATVAATRFEPLIRDFHQRPLSEG"
BASE COUNT          112 a          112 c          126 g           80 t
ORIGIN      
        1 cggcggatat gcggactgcc gagaaaaacc gtctgcatca ggttcacgaa acgcaagtcg
       61 gaagcgtcaa acaactgatt gcccattttg accggctgat tgacgaattg gacaaacaaa
      121 tcgacaacca cacccacacg cattttgacg gcaaagacca agtggcggag caaatcaaag
      181 gcatcggttc gataacgacg gctacgctga tggcgatgtt gcccgaattg gggcggctgt
      241 tgcacaaacg gatagcgggt ttggccggca ttgccccgca cccgagggag ggcggggaaa
      301 ccaaattcaa aagccgctgc tttggcggaa ggtctgcggt gcgtaaagca ctgtatatgg
      361 ctaccgtggc agcgacacgt tttgaaccgc ttattcggga tttccaccaa cgcccgctgt
      421 ccgagggtaa
//
LOCUS       DAWFBO010000276          430 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWFBO010000276 DAWFBO010000000
VERSION     DAWFBO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.276"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           84 a           96 c          126 g          124 t
ORIGIN      
        1 gggatttagg gctaatctag tacagcccct tagatttaat tcactatacc tgcgtaatgg
       61 tatcgggtac tcatcatcaa cctgccttgc attcggttgt gttgtccggc aactgtccgg
      121 ttgtgtcgat gggttgccct gccgctccct gtgctgcgca acggttgtgt gccttgggtg
      181 ggcgcgggtg gcggccgggc ggtttgttgc tatgatacgg gagttccgat atacaagggt
      241 gggcttcagc ccaccaatca acgaccgaaa aagaaaaaac gttgggctga agcccactct
      301 acgcaattac gcaactgtta tcgtggggat gacgggattt taggtttttg tttttctgtt
      361 tttgcgggaa ggatgggttt taggttgcgg tcatttatcg gaaaagcaga aatcgctccg
      421 ccgtcattcc
//
LOCUS       DAWFBO010000277          428 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWFBO010000277 DAWFBO010000000
VERSION     DAWFBO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.277"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          125 a          105 c          116 g           82 t
ORIGIN      
        1 gcgggaatga cggcgcataa gttcccgtgc ggacagacct agattcccac ctgcgtggga
       61 atgacgatgc agaagttgcc cgaaactcaa aaaaaacgaa accgaacgaa ccggattccc
      121 gctttcgcgg gaatgacggc gcataagttc ccgtgcggac agacctagat tcccacctgt
      181 gtgggaatga cgattcagaa gttgcccgaa accaaaaaac tgaagccgaa cggtctggat
      241 tcccgctttc gcgggaatga cggcatatca gtttctgtgc ggacaaacct ggattcccat
      301 ctgcgtagga atgacgatgc agaagttgcc cgaaactcaa aaaaaacgaa accgaacgaa
      361 ccggattccc gcttttacgg gaatgacggg attttgggtt gtggtaattt atcgggaaaa
      421 cggaaacc
//
LOCUS       DAWFBO010000278          428 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWFBO010000278 DAWFBO010000000
VERSION     DAWFBO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.278"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>428
                     /locus_tag="WGO87_002019"
     CDS             <1..>428
                     /locus_tag="WGO87_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0393323.1"
                     /translation="YRNRLAQDDTLSELLKLINRQLTEKGLKIEKASAAVIDATIIQT
                     AGSKQRQAIEVDEEGQISGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLH
                     ITPANAHECKHLPPLLEGIAEGTTVYADKGYDSAENRQHL"
BASE COUNT          141 a          120 c          101 g           66 t
ORIGIN      
        1 taccgcaacc ggctggcgca agacgacacc ctgtccgaat tgctcaaact gattaaccga
       61 caactgaccg aaaaaggttt aaaaatagag aaagcatccg ccgccgtcat tgacgccacc
      121 attattcaga ccgctggcag caaacagcgt caggccatag aagttgacga agaaggacaa
      181 atcagcggcc aaaccacacc gagtaaggac agcgatgccc gttggataaa gaaaaacggc
      241 ctctacaaac tcggttacaa acaacatacc cgtaccgatg cggaaggcta tatcgagaaa
      301 ctgcacatca cccccgccaa tgcccatgag tgcaaacacc tgccgccgtt gttagaaggg
      361 atagctgaag gtacgaccgt ctatgccgac aaaggctatg acagtgcgga aaaccggcaa
      421 catctgga
//
LOCUS       DAWFBO010000279          422 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWFBO010000279 DAWFBO010000000
VERSION     DAWFBO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.279"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(344..419)
                     /locus_tag="WGO87_002020"
     tRNA            complement(344..419)
                     /locus_tag="WGO87_002020"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(384..386),aa:Ala,seq:tgc)
BASE COUNT          123 a           83 c           75 g          141 t
ORIGIN      
        1 gtttacctac ccgttgacta gggaagtaaa cttgaaattc ctactttgat aaagcttact
       61 gctttgttgt gtcttaatcc tgccttttgt gtttcaggat taagtcgata caatcatcac
      121 ccaaatactg tgtttgtttt cttttctctc gcgagagatt tttacccttt gcaaaaggta
      181 aaaatcaaaa caaactcatt gtctttgttt gttgatttcg gctttccaat ttgttaaaga
      241 tcgatgcgtc gttattttac tccgcaaatc aaaataagtt gctataaaat ataaaagtac
      301 aaataaaata aaagcaacag gattactttg atttgcaaag ttttggtgga ggcaaacggg
      361 atcgaaccga tgaccccctg cttgcaaagc aggtgctcta ccaactgagc tatgcccccg
      421 tt
//
LOCUS       DAWFBO010000280          417 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWFBO010000280 DAWFBO010000000
VERSION     DAWFBO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.280"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>417
                     /locus_tag="WGO87_002021"
     CDS             <1..>417
                     /locus_tag="WGO87_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0393324.1"
                     /translation="GQDAYSINLIEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGL
                     DSVDDIVIRKDACHLNQDLGKSLLDLGFKKIPSVYPEYEAYEDKRSIPENPYIHELQY
                     IKKGENPAIITHQNYHRYGENDYSTDIGSCINGFTV"
BASE COUNT          140 a           73 c           85 g          119 t
ORIGIN      
        1 ccgggcagga tgcttactcc attaatttga tagagattag cgtctattac aaaaaaaccg
       61 accaaggttg ggttcttgag ccatacaacc agcaaaacaa agcacacttt attcaatttc
      121 tacgcgatgg tttggatagc gtggacgata ttgttatccg aaaagatgcg tgtcatttga
      181 atcaagactt aggaaaatcg ttgcttgatt tgggatttaa aaaaatcccg tctgtctatc
      241 ctgaatatga agcttatgaa gataaaaggt cgattcctga aaacccatat attcatgaac
      301 tacagtatat taagaaagga gaaaatccgg cgattattac tcatcagaac tatcataggt
      361 atggagagaa cgattacagt actgatatag gttcctgtat taacggtttc acggtac
//
LOCUS       DAWFBO010000281          415 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWFBO010000281 DAWFBO010000000
VERSION     DAWFBO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.281"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            66..266
                     /locus_tag="WGO87_002022"
                     /pseudo
     CDS             66..266
                     /locus_tag="WGO87_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            313..>415
                     /locus_tag="WGO87_002023"
     CDS             313..>415
                     /locus_tag="WGO87_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0393325.1"
                     /translation="MTGFNDAAGVASSPSDIKGKYVQSVTVANGVITA"
BASE COUNT          108 a          133 c           96 g           78 t
ORIGIN      
        1 agcgcgacgg caccgccggc accaacaacg acgaagtcaa agccgccacc ggcaccaccg
       61 ccaacatcga caccaagcac ctgccgtcaa cctgccgcga tgattcatct accggttgca
      121 tagaaacacc acgcgccgac ttcaaacact tccaaaaaat cagccgttac cgagtattgc
      181 ccgaatcacg gcacatggcc gaaaaacttc gtcattcccg cgcaggcggg aatccaggtc
      241 tgtcggtacg gaaacttatc gggtaaaacg gtttcttgag attttgcgtt ctggattccc
      301 actttcgtgg gaatgacggg atttaatgat gccgccggcg tggcatcctc cccctccgac
      361 atcaaaggca aatatgttca aagcgttaca gtcgcaaacg gcgtcattac cgccc
//
LOCUS       DAWFBO010000282          405 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWFBO010000282 DAWFBO010000000
VERSION     DAWFBO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.282"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(178..>405)
                     /locus_tag="WGO87_002024"
     CDS             complement(178..>405)
                     /locus_tag="WGO87_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393326.1"
                     /translation="NVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSM
                     SVFGSNTAATGGNVDANTQSVQQPLLVTPSA"
BASE COUNT          131 a           80 c           78 g          116 t
ORIGIN      
        1 atagtacggt aaggcgaggc aacgctgtac tggtttttgt gaatccacta tattgctaga
       61 agtgcaccga ttaaggttaa ttaaaatacg acccttatcc caatattaag aaaaacagca
      121 atcaaaataa acttgattgc tgtttttcag tttaagccat gaatgtgatt aggctcctta
      181 tgcagatggc gttaccaata acggctgctg tacggattgt gtattggcat ccacatttcc
      241 tcctgtcgca gcagtattag aaccgaacac agacatagac tgtaccaaat tattgccagc
      301 attaatatac ttggcaaaat ctggattact gatagctgca tcatcaaaga caaatgtatc
      361 tacacgatgg ttttcaccat agaagaatcc ggaaatacgt acgtt
//
LOCUS       DAWFBO010000283          402 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWFBO010000283 DAWFBO010000000
VERSION     DAWFBO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.283"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            108..323
                     /gene="rpmE"
                     /locus_tag="WGO87_002025"
     CDS             108..323
                     /gene="rpmE"
                     /locus_tag="WGO87_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0393327.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          124 a          105 c           74 g           99 t
ORIGIN      
        1 attttatcga atatcctctt gacgaaacaa cagaaaatag cgagaatgcc gagcttgtct
       61 gaagcatagg tacatcccct atgcccgctt ttttgaaagg aaatcaaatg aaacaaggta
      121 ttcacccgaa ctaccacgaa gtcaacgtta cctgctcttg cggcaacaaa ttcgcaacca
      181 aatccgcgat ggaaaaagaa aacttcaata tcgaggtttg ctccctgtgc cacccgttct
      241 ataccggcac ccaaaaaatc gtcgatacca ccggccgcgt ggacaaattc aacaacaaat
      301 tcggcaacct gttcaaacgc tgattgccgt tttgcaagaa ttaagccctg ccatcatttc
      361 gatggcaggg ctttttaata tttggcacac tgacttctta tc
//
LOCUS       DAWFBO010000284          392 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWFBO010000284 DAWFBO010000000
VERSION     DAWFBO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.284"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            10..>392
                     /locus_tag="WGO87_002026"
     CDS             10..>392
                     /locus_tag="WGO87_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEZ0393328.1"
                     /translation="MLCPEKMSGMAGQYPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLA"
BASE COUNT           81 a           88 c          125 g           98 t
ORIGIN      
        1 cgggggtttt tgctttgccc ggaaaaaatg tcggggatgg cgggacagta tccgtacggc
       61 gtccggtcgg gtttgcggag gaacggcttg aaactttggg atattcattt tagaatgacc
      121 cgttttatcg tcgcaagatg cggtttattg tttgcaaccc ttaaaggaaa aaccatgaag
      181 aaaatgttcg tgctgttctg tatgctgttc tcctgcgcct tctcccttgc ggcggtaaac
      241 atcaatgcgg cttcgctgca ggagttggag gcgctgccag gcataggccc ggcgaaggcg
      301 aaggccattg cggaataccg tgcgcaaaac ggtgcgttca agtctgtgga cgatttgacc
      361 aaggtgaagg gcatcggccc tgcggtgctg gc
//
LOCUS       DAWFBO010000285          390 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWFBO010000285 DAWFBO010000000
VERSION     DAWFBO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.285"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          138 a           81 c           88 g           83 t
ORIGIN      
        1 aaaaaaaacg aaatcgaacg gactggattc ccgcctgcgc gggaatgacg agtcagaagt
       61 tactcgaaac ttaaaaaacc gaaatcgaac gaattggatg gattcccgcc tgcgcgggaa
      121 tgacgattga tacgtttcga taatttattg aaaatggctg aaatccaaag ttacagaaat
      181 ttataaacaa aaaacagcaa cccgctgccg tcattcccgc gaaagcggga atccagtccc
      241 ttgatcttaa gataatcttt aattcgtcat tcctgaaaaa gcgggaatcc ggaagcgaaa
      301 agtggtggga atgtattaga aatgactgaa actcaaaaaa ccggattccc gcctacgcgg
      361 gaatgacgag tcagaagtta ctcgaaactt
//
LOCUS       DAWFBO010000286          376 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWFBO010000286 DAWFBO010000000
VERSION     DAWFBO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.286"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..185)
                     /locus_tag="WGO87_002027"
     CDS             complement(<1..185)
                     /locus_tag="WGO87_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393329.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAAR"
     gene            complement(182..>376)
                     /locus_tag="WGO87_002028"
     CDS             complement(182..>376)
                     /locus_tag="WGO87_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0393330.1"
                     /translation="RGEDGFKPDNAGHHSRLAYAQAGGIVEPFFISNDTDLALFKTTK
                     WGICRAIADAIAMKLGAARV"
BASE COUNT           87 a           99 c           89 g          101 t
ORIGIN      
        1 cgcgccgccc gttttgcggc tgccacgcct gagtttctta aatttgggcg tggataggtg
       61 tgtatcggac gaacatttgg catctgtata agtgcgcggt ttacctgctc attactagga
      121 ttttctccaa tcaattttgt aagaaaatag gaagcagctt ctttcaattt accaataata
      181 ttcatactct tgccgccccc aatttcatcg caatcgcgtc cgcaatcgcg cggcagatgc
      241 cccatttggt cgtcttaaac aaggccaaat cagtgtcgtt gctgatgaaa aaaggctcaa
      301 caatgccgcc agcctgcgca taagccaggc gcgaatgatg gcccgcattg tcgggtttaa
      361 agccgtcttc gccgcg
//
LOCUS       DAWFBO010000287          364 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWFBO010000287 DAWFBO010000000
VERSION     DAWFBO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.287"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..283)
                     /locus_tag="WGO87_002029"
     CDS             complement(<1..283)
                     /locus_tag="WGO87_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0393331.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPIIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPS"
BASE COUNT           97 a           91 c          108 g           68 t
ORIGIN      
        1 aagaaggaac gtatgtaccc gaaacggcag gtgcagacgg cggaacataa gcaggaggcg
       61 tataaaccgg cgcgctttgc accggcggca cataaggcgc atcgccggca ggagccgggc
      121 tgtacggcgt tgctccatag gggttgttgt aaactgccga agacggcgcg tcctgcatac
      181 ctgaattgcc tgcaatgatg ggggcaggct gctgggtagc acaaccgccc aacagtgcgg
      241 caaaggaggc gcaagccgcc aaaagtgtcg tttgtttcaa cataagataa ccttcatgtt
      301 ccgatatata gcctgaatgc ggtatatcat aataaaaatg cgcgttcttc tcaagcgcaa
      361 agcc
//
LOCUS       DAWFBO010000288          353 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWFBO010000288 DAWFBO010000000
VERSION     DAWFBO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.288"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           92 a           92 c           70 g           99 t
ORIGIN      
        1 tcgttcccac ggtcaggatt tcagattgcg gacatctgtc agaaaagaca aaaaaccttc
       61 cgccgtcatt ccctacaggc gagaatccga tccgttgaaa ttcggttgtt ttaaataaat
      121 tcttgcagct ttgattttct gtttttccga taacgccgta actttgaaac gcgaaagcgg
      181 taatccgatc cgttgggatt ttgcaacttc aaatcaatcc gcaaactgaa atcccgtcat
      241 tcccgcgcag tcgtgaatcc gaacgcgtcc gcacgaaaac ctgcatcccg tcattcccac
      301 ggaagtggga atccagtttt ttgagtttca gtcattcccg ataaattgcc tta
//
LOCUS       DAWFBO010000289          351 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWFBO010000289 DAWFBO010000000
VERSION     DAWFBO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.289"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            26..>351
                     /locus_tag="WGO87_002030"
     CDS             26..>351
                     /locus_tag="WGO87_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0393332.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTVKNKRWCQ
                     VLSKAVA"
BASE COUNT           91 a           81 c          105 g           74 t
ORIGIN      
        1 aatcaatatt ttttggagat gatgaatggg caaaaccgta accttaaccg ctggacacag
       61 caacaccgac ccgggtgcgg tcaacggcag cgaccgtgag gcggacttgg cgcaggatat
      121 gcgcaacatc gtggctgcta ttttgcgcga tgactacggt ttgactgtta aaaccgacgg
      181 cacaggcaaa ggcaatatgc cgctgcgtga agctgtaaaa ctgattcgcg gctcggatgt
      241 ggcgattgag ttccatacca actcggcggc cggtaaagca gctacgggca ttgaggcgtt
      301 gagtaccgtt aaaaacaaac gctggtgtca ggtgttgagc aaagctgttg c
//
LOCUS       DAWFBO010000290          347 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.290, whole genome shotgun sequence.
ACCESSION   DAWFBO010000290 DAWFBO010000000
VERSION     DAWFBO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.290"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT          122 a           85 c           63 g           77 t
ORIGIN      
        1 actcattagt tacctaaaac ttaaaaaaca gaaaccttta cgccgtcatt cccacgaaag
       61 tgggaatcta gaacccaaat gctaaggcga tttatcggaa acggctgaaa ccgaacgaat
      121 tggattcccg cctgcgcagg aatgacaact cattagttac ctaaaactta aaaaacagaa
      181 acctttacac cgtcattccc acgaaagtgg gaatctagaa cccaaatgct aaggcgattt
      241 atcggaaacg gctgaaaccg aatgaattgg attcccgcct gtgcgggaat gacgacttat
      301 tagttaccta acacttaaaa aacagaaacc tttccgcgtc attccca
//
LOCUS       DAWFBO010000291          346 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.291, whole genome shotgun sequence.
ACCESSION   DAWFBO010000291 DAWFBO010000000
VERSION     DAWFBO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.291"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..152)
                     /locus_tag="WGO87_002031"
     CDS             complement(<1..152)
                     /locus_tag="WGO87_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393333.1"
                     /translation="MKKSLIALALSVLKPQMPEFEIKPASIGYLKQHPSMRLGKSGVA
                     AAKRAAR"
     gene            complement(149..>346)
                     /locus_tag="WGO87_002032"
     CDS             complement(149..>346)
                     /locus_tag="WGO87_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0393334.1"
                     /translation="RGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
BASE COUNT           85 a          104 c           81 g           76 t
ORIGIN      
        1 cgcgccgcgc gtttcgcggc tgccacgccc gacttgccta ggcgcataga cggatgttgt
       61 ttcaaatagc caatactggc aggcttaatc tcaaattcag gcatctgcgg tttcaagaca
      121 gacagagcca aagcaatcaa agactttttc ataccttcgc cgctcccaat tccatcgcaa
      181 tcgcgtccgc aatcgcgcgg cagatgcccc atttagtagt cttaaacaag gccaaatcag
      241 tgtcgttgct gatgaaaaaa ggctcaaaca caatgccacc tgcctgcgca taagccaggc
      301 gcgaatgctg gcccgcattg tctggtttaa agccgtcttc gccgcg
//
LOCUS       DAWFBO010000292          331 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.292, whole genome shotgun sequence.
ACCESSION   DAWFBO010000292 DAWFBO010000000
VERSION     DAWFBO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.292"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            41..331
                     /locus_tag="WGO87_002033"
                     /pseudo
     CDS             41..331
                     /locus_tag="WGO87_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          103 a           93 c           71 g           64 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat actttatgcc gctaccgcaa
       61 ctggcggctc aagacaatac cctgtccgaa ttgctcaaac tgattaaccg acaactgacc
      121 gaaaaaggcc taaaagtaga gaaagcatcc gccgccgttg ttgacgccac cattattcag
      181 accgccggca gcaaacagcg tcaggctata gaagtcgatg aagaaggaca agtcagcggc
      241 caaaccacac cgagtaaaga cagcgatgcc cgttggataa agaaaaacgg cctctacaaa
      301 ctcggttaca aacaacatac ccgtaccgat g
//
LOCUS       DAWFBO010000293          328 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.293, whole genome shotgun sequence.
ACCESSION   DAWFBO010000293 DAWFBO010000000
VERSION     DAWFBO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.293"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           91 a           79 c           87 g           71 t
ORIGIN      
        1 cccctctccc tgtgggagag ggttagggag agggcagtaa gccgtaggct tacatcaaag
       61 tcgataacgc tttcacaaag cctgtgtgca ctgaaaactg aaaggtcatc tgaaaacgga
      121 gctttcgaag gaagcagagt ttctgcgaag ctaaaaccga agctgcaaaa aatcgaaaag
      181 cagcctgcac gttgaaaatg ccgcccaagc aaacctgcgg tttgccgccc tctccctagc
      241 cctctcccac agggagaggg gattgggttg caggctgcct ttaaggttta ggcaaatttt
      301 taacttcgtt gaagctgcga tttcagaa
//
LOCUS       DAWFBO010000294          325 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.294, whole genome shotgun sequence.
ACCESSION   DAWFBO010000294 DAWFBO010000000
VERSION     DAWFBO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.294"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>325
                     /locus_tag="WGO87_002034"
     CDS             <1..>325
                     /locus_tag="WGO87_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0393335.1"
                     /translation="KSQIKFQSSNNLKSSGFKLDSSSKSFAKFIEAANFEHIPTGAKA
                     RINAKITASVSRAGVLAGVGLGAKFSTKAVPYVGTALLAHDVYETFKEDIQAQGYQYD
                     PETDKF"
BASE COUNT          111 a           77 c           66 g           71 t
ORIGIN      
        1 aaatcacaaa ttaaatttca atcaagtaat aatttaaaat caagtggttt taaattagat
       61 tcaagttcta agagttttgc caaattcata gaagcggcaa attttgagca catccctacg
      121 ggcgcaaaag cccgaatcaa cgcaaaaata accgccagcg tatcccgcgc cggcgtattg
      181 gcgggggtcg gcttaggcgc gaaattcagc acaaaggcgg ttccctatgt cggaacagcc
      241 cttttagccc atgacgtata cgaaactttc aaagaagaca tacaggcaca aggctaccaa
      301 tacgaccccg aaaccgacaa atttg
//
LOCUS       DAWFBO010000295          318 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.295, whole genome shotgun sequence.
ACCESSION   DAWFBO010000295 DAWFBO010000000
VERSION     DAWFBO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.295"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           90 a           88 c           66 g           74 t
ORIGIN      
        1 acggaaacct gcaccgcgtc attcccacga acccacatcc cgtcattacc acgaaagcgg
       61 gaatctagga cgcagggtta agaaaacctg catcccgtca ttcctcaaaa acagaaaacc
      121 aaaatcagaa acctaaaatc ccgtcattcc cgcgcaggcg ggaatctagg tctgtcagtg
      181 caggaactta tcggataaaa cggtttcttg agattttaca tcctggattc ccactttcgt
      241 gggaatctag aatctcggac tttcagataa tctttgaata ttgccgctgc cttaaggtct
      301 agattcccgc ctgcgcgg
//
LOCUS       DAWFBO010000296          318 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.296, whole genome shotgun sequence.
ACCESSION   DAWFBO010000296 DAWFBO010000000
VERSION     DAWFBO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.296"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           88 a           84 c           67 g           79 t
ORIGIN      
        1 ctttcgtggg aatgacggga tttgagattg cggcatttac cggaaaaaac agaaaccgct
       61 ctgccgtcat tcccgcgcag gcgggaatct agaccttaga acaacagtaa tattcaaaga
      121 ttatctgaaa gtccgggatt ctggattccc actttcgtgg gaatgacggc atcagtctgc
      181 cgtttacagc acggtttctt tagattttac gttctagatt cctgcctgcg cgggaatgac
      241 gaatccatcc atacggaaac ctacaccacg tcattcctac gaacctacat cccgtcattc
      301 ccacaaaaac agaaacct
//
LOCUS       DAWFBO010000297          314 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.297, whole genome shotgun sequence.
ACCESSION   DAWFBO010000297 DAWFBO010000000
VERSION     DAWFBO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.297"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            175..>314
                     /locus_tag="WGO87_002035"
     CDS             175..>314
                     /locus_tag="WGO87_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0393336.1"
                     /translation="MHYKLIELFVAGVTARKAAELVGVNKNTAAYYFHRLRLLIYQNS
                     PH"
BASE COUNT           86 a           66 c           71 g           91 t
ORIGIN      
        1 ggcgaaaaca gccgaaaacc tgtgtttggg tttcggctgt cgggagggaa aggaattttg
       61 caaaggtctc aaatagcacg gaaccgattc acttggtgct tgagcaccaa gtgaatcggt
      121 tccgtgctat ttgtactgcc tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac
      181 tataaactca ttgaactgtt tgtcgcaggc gtaactgcaa gaaaggcagc agagttagta
      241 ggcgttaata aaaataccgc agcctattat tttcatcgtt tacgattact tatttatcaa
      301 aacagcccac attt
//
LOCUS       DAWFBO010000298          308 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.298, whole genome shotgun sequence.
ACCESSION   DAWFBO010000298 DAWFBO010000000
VERSION     DAWFBO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.298"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            18..212
                     /gene="thiS"
                     /locus_tag="WGO87_002036"
     CDS             18..212
                     /gene="thiS"
                     /locus_tag="WGO87_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0393337.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT           85 a           90 c           77 g           56 t
ORIGIN      
        1 agaaacatcg agaaaccatg aacatcatct taaacggcgg acccgccgaa cttcacggca
       61 caagcgttgc cgacctcatc gcccaaaccg cgccgcaaaa gccctttgcc gtggcggtca
      121 acaccgtttt cgtccccaaa ggcgcgtatg cggaaacggt tttaaacgaa aacgacaaaa
      181 tcgatatcgt gcggccggtg gtcggcggct aggcggtttt gccttttcag acgacccctg
      241 tccccaaaac aacgttatgg tggattaact ttaaatcagg acaaggcgac gaagccgcag
      301 acagtacg
//
LOCUS       DAWFBO010000299          296 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.299, whole genome shotgun sequence.
ACCESSION   DAWFBO010000299 DAWFBO010000000
VERSION     DAWFBO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.299"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           76 a           68 c           92 g           60 t
ORIGIN      
        1 cggaaaaaac agaaaccgct ccgccgtcat tcccgcgcag gcgggaatct aggacgtaaa
       61 atctcaagaa accgttgtac ccgataagtt tccgtgccga caagtccgga ttcccgcctg
      121 cgcgggaatg acggttcagt tgcgtaggac tggattgtga aaaggggcgg attcggtgaa
      181 aacggcggaa atgtgggatt gatggaatcg gtgggctgaa gccctcccta cagagctttc
      241 agacggtatt gtttgcgttt tcgggatggg ggcaaatgaa acgccgacaa accgat
//
LOCUS       DAWFBO010000300          293 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.300, whole genome shotgun sequence.
ACCESSION   DAWFBO010000300 DAWFBO010000000
VERSION     DAWFBO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.300"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            <1..>293
                     /locus_tag="WGO87_002037"
     CDS             <1..>293
                     /locus_tag="WGO87_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0393338.1"
                     /translation="HLLPLLEGIAEGTTVYADKGYDSKENRQHLKEHQLLDGIMRKAC
                     RNRPLSETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLLKVSAQSHL"
BASE COUNT           79 a           79 c           78 g           57 t
ORIGIN      
        1 cacctgttgc ctttgctgga aggtatagcc gaaggcacga ccgtctatgc cgacaaaggc
       61 tacgacagta aggaaaaccg gcagcatctg aaagaacatc agttactgga cggcattatg
      121 cgcaaagcct gccgcaaccg tccgctgtcg gaaacgcaaa ccaaacgcaa ccggtatttg
      181 tcgaagaccc gttatgtggt tgaacagagc ttcggtacgc tgcaccgtaa attccgctat
      241 gcgcgggcag cctatttcgg gctgctcaaa gtgagtgcgc aaagccatct gaa
//
LOCUS       DAWFBO010000301          276 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.301, whole genome shotgun sequence.
ACCESSION   DAWFBO010000301 DAWFBO010000000
VERSION     DAWFBO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.301"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           92 a           65 c           65 g           54 t
ORIGIN      
        1 tggatggatt cgtcgttccc gcgaaagcgg gaatccggaa ccagaaacca caaaaattta
       61 tcgggaaaac cgaaactcaa aaacaacgga aaccgaacgg acaggattcc cgcctgcgcg
      121 ggaatgacgg ctcatacatc acccaaaaaa ttgaaaccaa acagatggga ttccctcttg
      181 cgtgaggctg acagatgccg tctgaaagac tttcagacgg catagctttt tctctttgaa
      241 tttatagtgg attaacaaaa atcaggacaa ggcggc
//
LOCUS       DAWFBO010000302          269 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.302, whole genome shotgun sequence.
ACCESSION   DAWFBO010000302 DAWFBO010000000
VERSION     DAWFBO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.302"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           59 a           50 c           81 g           79 t
ORIGIN      
        1 atttatcgga aatgaccgaa actgaacgga ctggattccc gcttttgcgg gaatgacggt
       61 tcagttgcta cggttattgt caggtttcgg ttatgttgga atttcgggaa acttatgaat
      121 cgtcattccc gcgcaggcgg gaatctagac cggtcggtgc ggaaacttat cgggaaaaaa
      181 ggtttcttga gattttacgt cctggattcc cactttcgtg ggaatgacgt ggtgcaggtt
      241 cgtaggaatg acgtggtgca ggtttccgt
//
LOCUS       DAWFBO010000303          267 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.303, whole genome shotgun sequence.
ACCESSION   DAWFBO010000303 DAWFBO010000000
VERSION     DAWFBO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.303"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            41..211
                     /locus_tag="WGO87_002038"
     CDS             41..211
                     /locus_tag="WGO87_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0393339.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT           71 a           71 c           71 g           54 t
ORIGIN      
        1 ggtttttgtt catccgctat acaaaaacaa ggaaacacaa atggcgcaat atatgtgcgg
       61 cccctgcggc tggatttacg atgaagaaca cggcgaccct gaacacggca tcccccccgg
      121 gacaaagttt gaagacattc ccgacgactg gaaatgcccc gaatgcggcg tgggcaaaga
      181 agatttctac ctgttggatt tcgtgatata gcggcagccg aaccccttcg gaaaattcct
      241 cggcgttccg cctgcggtag cagggaa
//
LOCUS       DAWFBO010000304          258 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.304, whole genome shotgun sequence.
ACCESSION   DAWFBO010000304 DAWFBO010000000
VERSION     DAWFBO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.304"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           61 a           51 c           76 g           70 t
ORIGIN      
        1 ggatgaattc gtcattcccg cgcaggcggg aatctagaca ttcaatgcta aggcaattta
       61 tcgggaatga ctgaaactca aaaaaactgg attcccactt ttgtgggaat ggcgggatgt
      121 aggttcgtgg gaatgacggg atgcaggttt ccgtatggat ggattcgtca ttcccgcgca
      181 ggcgggaatc caggtctgtc ggtgcggaaa cttatcgggt aaaacggttt cttgagattt
      241 tgcgtcttgt attcccac
//
LOCUS       DAWFBO010000305          253 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.305, whole genome shotgun sequence.
ACCESSION   DAWFBO010000305 DAWFBO010000000
VERSION     DAWFBO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.305"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(20..>253)
                     /locus_tag="WGO87_002039"
     CDS             complement(20..>253)
                     /locus_tag="WGO87_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0393340.1"
                     /translation="LIAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNIWKIYFSDES
                     VIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
BASE COUNT           97 a           42 c           26 g           88 t
ORIGIN      
        1 tacaggttca gaataagaat tactcgaaca tagaattaaa atcagaacta tcggtgtact
       61 cttgataaac aaattcatga acagtttttc cattctcttc tgattttctt ataaaaataa
      121 cagattcatc agaaaaataa atcttccaaa tattatgtgc gactctctta tttaaaaaat
      181 aagagaagct ctctaaaata tcgtactctt ttacgttatt aacaaattct tcaaattctt
      241 tctgagcaat taa
//
LOCUS       DAWFBO010000306          251 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.306, whole genome shotgun sequence.
ACCESSION   DAWFBO010000306 DAWFBO010000000
VERSION     DAWFBO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.306"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           70 a           59 c           59 g           63 t
ORIGIN      
        1 cgcttgttcg gtaaatgaga gggcggatta cgcgcctgtc agataaaccc cgtgtttaaa
       61 cgggcggcaa tgaggtacgc gtagagcctt gaagcgcaat cgatatatta tttccaccaa
      121 aacggacgac cccgcctgcc ttgcaaaccc ttaaaatgaa gccacccgga ttaatccgag
      181 tggccgtgga aaatcactta ccgcttgatt tatttaaaat ttatggtata atttacctta
      241 gctggcagat g
//
LOCUS       DAWFBO010000307          250 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.307, whole genome shotgun sequence.
ACCESSION   DAWFBO010000307 DAWFBO010000000
VERSION     DAWFBO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.307"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           56 a           81 c           55 g           58 t
ORIGIN      
        1 aatcttgaaa tccgtcattc ccgcgcaggc gggaatccag acctgtccgc acagaaactt
       61 atcggataaa aacagttgct caaacccctc gttctagatt cccacttccg tgggaatgac
      121 ggttcagttg ctacggcccc gccctacaat acgccttgcg aatctgtccc acgcccgccc
      181 ttgtatgcct caaatgggtt aaactacgca cttcccgact tcccgtttcg ggcaggggta
      241 caggtccgat
//
LOCUS       DAWFBO010000308          250 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.308, whole genome shotgun sequence.
ACCESSION   DAWFBO010000308 DAWFBO010000000
VERSION     DAWFBO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.308"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           75 a           54 c           63 g           58 t
ORIGIN      
        1 gaatgacggg atgtgggttc gtgggaatga cgaagtagaa gttacccgaa atttaaaaca
       61 agcgaaaccg aacgaactgg attcccgctt tcgtgggaat gacggcggag cggtttctgc
      121 ttttcccaat aaatgcccca acctaaaatc cgtcattcct gcgcaggcgg gaatctagac
      181 attcaatgct aaggcaattt atcgggaatg actgaaactc aaaaagctag attcccactt
      241 tcgtgggaat
//
LOCUS       DAWFBO010000309          242 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.309, whole genome shotgun sequence.
ACCESSION   DAWFBO010000309 DAWFBO010000000
VERSION     DAWFBO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.309"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            complement(<1..232)
                     /locus_tag="WGO87_002040"
     CDS             complement(<1..232)
                     /locus_tag="WGO87_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0393341.1"
                     /translation="MYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKEQTSFNNP
                     EPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDN"
BASE COUNT           71 a           46 c           47 g           78 t
ORIGIN      
        1 cattgtcttg cgtataccgt gcaagatagc cataagggat aaccattttg gtgccctgaa
       61 aatcaaatgt aaccgtatgt tcaaatcctg tcattggctc gggattgttg aaactggttt
      121 gttctttagc ctgttgcgca cttactgctt tttgatctgt cttttcattc acaggttcac
      181 atgaggtcat agtaaaaaca ctcccaataa acaaaataaa aagttgataa atggtagtag
      241 ca
//
LOCUS       DAWFBO010000310          239 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.310, whole genome shotgun sequence.
ACCESSION   DAWFBO010000310 DAWFBO010000000
VERSION     DAWFBO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.310"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
     gene            36..>239
                     /locus_tag="WGO87_002041"
     CDS             36..>239
                     /locus_tag="WGO87_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0393342.1"
                     /translation="MLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMTRL
                     PTFLCNDVVKNPQVEKYLADYQAH"
BASE COUNT           67 a           51 c           59 g           62 t
ORIGIN      
        1 acggcacagg cggcttgttg caaggcaaaa aacatatgct ttcactgact tggaatgcgc
       61 cgattgaggc gtttacccgc gaaggcgatt tctttgaagg caaaggcgtt gatgttttgt
      121 atatgcactt ccacaaagcc aacgagtttt tgggtatgac ccgcctgccg acattcttat
      181 gtaacgatgt ggttaaaaat ccacaagtgg aaaaatactt ggcagattat caggcacac
//
LOCUS       DAWFBO010000311          228 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain 2590STDY5514733 isolate 2978
            SAMEA2063860-rid20300973.denovo.311, whole genome shotgun sequence.
ACCESSION   DAWFBO010000311 DAWFBO010000000
VERSION     DAWFBO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2063860
            Sequence Read Archive: ERR332305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995148.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:57:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,041
            CDSs (total)                      :: 1,985
            Genes (coding)                    :: 1,903
            CDSs (with protein)               :: 1,903
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2063860-rid20300973.denovo.311"
                     /strain="2590STDY5514733"
                     /serovar="W"
                     /isolate="2978"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="Ghana"
                     /collection_date="2010"
BASE COUNT           76 a           56 c           62 g           34 t
ORIGIN      
        1 aaaagcaaac accgtccgtc gtgttgccgt ttgcggatga gtacgggtca accccaatgc
       61 cgccgaaacc gtcgaagccg ccttcaacat tgccgccgcc aaagccgcaa agttggcaaa
      121 aacggtaaaa ccggggagat aaaagccgat ggcaggaaag taaatgtgag gatagacagt
      181 acggaggcag acctgcttta tccggcaggg caataagaaa acaaaaat
//
